LOCUS       DATURV010000001        94385 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DATURV010000001 DATURV010000000
VERSION     DATURV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 94385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94385
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.001"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            155..421
                     /locus_tag="VCF75_000001"
     CDS             155..421
                     /locus_tag="VCF75_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEP2545605.1"
                     /translation="MLRDLLKGTLRFGQLKSSIGSVSQKVLTAQLRAMEADGLVHREV
                     YAEVPPWVEYSLTETGLSLAPVIEAMSDWGQTYQEKHADLFEEG"
     gene            complement(700..1413)
                     /gene="treR"
                     /locus_tag="VCF75_000002"
     CDS             complement(700..1413)
                     /gene="treR"
                     /locus_tag="VCF75_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEP2545606.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIRHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            1624..3648
                     /gene="treP"
                     /locus_tag="VCF75_000003"
     CDS             1624..3648
                     /gene="treP"
                     /locus_tag="VCF75_000003"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEP2545607.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPATEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFSAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANAIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLPETPVSE
                     APAATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGNLPHQIKANDKLVVAMKK"
     gene            3716..5344
                     /gene="treC"
                     /locus_tag="VCF75_000004"
     CDS             3716..5344
                     /gene="treC"
                     /locus_tag="VCF75_000004"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEP2545608.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMGQGNGWNALFYNNHDQPRALNRFIDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAK
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILAEN"
     gene            5552..7447
                     /gene="pepO"
                     /locus_tag="VCF75_000005"
     CDS             5552..7447
                     /gene="pepO"
                     /locus_tag="VCF75_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEP2545609.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGDHVPDDAILANFVKFHRLVADYAKRDEVGVSPILPLIAEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPNTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYS
                     KKVIDESLSLVENAQNLAKITIAHTWSKWSKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKINGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(7613..8653)
                     /locus_tag="VCF75_000006"
     CDS             complement(7613..8653)
                     /locus_tag="VCF75_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEP2545610.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(8787..9554)
                     /gene="rpsB"
                     /locus_tag="VCF75_000007"
     CDS             complement(8787..9554)
                     /gene="rpsB"
                     /locus_tag="VCF75_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEP2545611.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADTQADSIEEIVEVVEGDNA"
     gene            9858..12932
                     /locus_tag="VCF75_000008"
     CDS             9858..12932
                     /locus_tag="VCF75_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2545612.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALIILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGCLESICL
                     EPFNRQESLAYINSQLGSQPVTEEEMEHLYQASQGTPFFLSEYTQALLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDVLLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMQE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTSGLQANYAIQIAAALDYLGEIAQIRYQFEKAVAY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIRQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(12963..13949)
                     /locus_tag="VCF75_000009"
     CDS             complement(12963..13949)
                     /locus_tag="VCF75_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEP2545613.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSSSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYRMGYQKVCQTIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPK
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHN"
     gene            complement(14135..15676)
                     /gene="hutH"
                     /locus_tag="VCF75_000010"
     CDS             complement(14135..15676)
                     /gene="hutH"
                     /locus_tag="VCF75_000010"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEP2545614.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGIACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLIEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KSENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASAVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(15687..17030)
                     /locus_tag="VCF75_000011"
     CDS             complement(15687..17030)
                     /locus_tag="VCF75_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEP2545615.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNIGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVIFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(17250..17846)
                     /locus_tag="VCF75_000012"
     CDS             complement(17250..17846)
                     /locus_tag="VCF75_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEP2545616.1"
                     /translation="MTTVMVKTPADYTQTDWSGGETNQLFLYPEKGDYKERTFSYRLS
                     TATVALRQTTFTRLDGYHRILMSLDKPITLSDLSHAKEISLAPFEPYAFEGDTRIVSR
                     GTCQDINLMYDDHYQGQLLPVWSDTLFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCPNQTSPAQTPVAVWAGLWYKT"
     gene            complement(17868..19541)
                     /locus_tag="VCF75_000013"
     CDS             complement(17868..19541)
                     /locus_tag="VCF75_000013"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP2545617.1"
                     /translation="MVLSDIEIANSVTMEPISKVANQLGIDEEALCLYGKYKAKIDAR
                     QLVALKDKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYDADDSIETKLT
                     KIVTKVYGGKGITLTPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFI
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(19559..20185)
                     /locus_tag="VCF75_000014"
     CDS             complement(19559..20185)
                     /locus_tag="VCF75_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEP2545618.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQAIITEIHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     SAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(20196..21095)
                     /gene="ftcD"
                     /locus_tag="VCF75_000015"
     CDS             complement(20196..21095)
                     /gene="ftcD"
                     /locus_tag="VCF75_000015"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEP2545619.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATTKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTA
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEIIGLAPAKALIDV
                     AEYYLQVEDFDYHKQILENHLLG"
     gene            complement(21184..23214)
                     /locus_tag="VCF75_000016"
     CDS             complement(21184..23214)
                     /locus_tag="VCF75_000016"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEP2545620.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRWRPKDRIYGKPINEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFKSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVTQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            23506..24771
                     /locus_tag="VCF75_000017"
     CDS             23506..24771
                     /locus_tag="VCF75_000017"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEP2545621.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(24829..26361)
                     /gene="ahpF"
                     /locus_tag="VCF75_000018"
     CDS             complement(24829..26361)
                     /gene="ahpF"
                     /locus_tag="VCF75_000018"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEP2545622.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     ADTANMTIIKNVATKDIVGDDHVTGLNYTERDSGEDKHLDLEGVFVQIGLIPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(26382..26942)
                     /gene="ahpC"
                     /locus_tag="VCF75_000019"
     CDS             complement(26382..26942)
                     /gene="ahpC"
                     /locus_tag="VCF75_000019"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEP2545623.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            27350..27420
                     /locus_tag="VCF75_000020"
     tRNA            27350..27420
                     /locus_tag="VCF75_000020"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27382..27384,aa:Cys,seq:gca)
     gene            27939..28142
                     /locus_tag="VCF75_000021"
     CDS             27939..28142
                     /locus_tag="VCF75_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEP2545624.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            28338..28799
                     /locus_tag="VCF75_000022"
     CDS             28338..28799
                     /locus_tag="VCF75_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEP2545625.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            28799..31243
                     /locus_tag="VCF75_000023"
     CDS             28799..31243
                     /locus_tag="VCF75_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP2545626.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSYGKLTFHIA"
     gene            31421..31711
                     /gene="groES"
                     /locus_tag="VCF75_000024"
     CDS             31421..31711
                     /gene="groES"
                     /locus_tag="VCF75_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEP2545627.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            31747..33378
                     /gene="groL"
                     /locus_tag="VCF75_000025"
     CDS             31747..33378
                     /gene="groL"
                     /locus_tag="VCF75_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEP2545628.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            33629..33895
                     /locus_tag="VCF75_000026"
     CDS             33629..33895
                     /locus_tag="VCF75_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2545629.1"
                     /translation="MNHFGEIFKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMSIDEFMYAVHDFHRDDLNELLSKSEGFRNNSR"
     gene            complement(34623..36122)
                     /locus_tag="VCF75_000027"
     CDS             complement(34623..36122)
                     /locus_tag="VCF75_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP2545630.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDLSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQYKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVEEKPKNKKGLNRS"
     gene            36369..38009
                     /locus_tag="VCF75_000028"
     CDS             36369..38009
                     /locus_tag="VCF75_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545631.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLISGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALVPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(38098..39099)
                     /locus_tag="VCF75_000029"
     CDS             complement(38098..39099)
                     /locus_tag="VCF75_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP2545632.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTQEDICVQAKSATFDAVLNAQKARK"
     gene            39675..40055
                     /locus_tag="VCF75_000030"
     CDS             39675..40055
                     /locus_tag="VCF75_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP2545633.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            40094..42430
                     /locus_tag="VCF75_000031"
     CDS             40094..42430
                     /locus_tag="VCF75_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP2545634.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKARLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            42479..42631
                     /gene="rpmG"
                     /locus_tag="VCF75_000032"
     CDS             42479..42631
                     /gene="rpmG"
                     /locus_tag="VCF75_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP2545635.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            42645..42821
                     /gene="secE"
                     /locus_tag="VCF75_000033"
     CDS             42645..42821
                     /gene="secE"
                     /locus_tag="VCF75_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEP2545636.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(43057..43830)
                     /locus_tag="VCF75_000034"
     CDS             complement(43057..43830)
                     /locus_tag="VCF75_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEP2545637.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            43956..44702
                     /locus_tag="VCF75_000035"
     CDS             43956..44702
                     /locus_tag="VCF75_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP2545638.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLEVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKVITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            44718..45698
                     /locus_tag="VCF75_000036"
     CDS             44718..45698
                     /locus_tag="VCF75_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEP2545639.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRKQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            45986..46306
                     /locus_tag="VCF75_000037"
     CDS             45986..46306
                     /locus_tag="VCF75_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP2545640.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            46334..46642
                     /locus_tag="VCF75_000038"
     CDS             46334..46642
                     /locus_tag="VCF75_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2545641.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIQMADYGMLDGNKVLDLALSLVKE
                     "
     gene            46652..47956
                     /locus_tag="VCF75_000039"
     CDS             46652..47956
                     /locus_tag="VCF75_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP2545642.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSDVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGLIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQATEES"
     gene            48167..50584
                     /locus_tag="VCF75_000040"
     CDS             48167..50584
                     /locus_tag="VCF75_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEP2545643.1"
                     /translation="MTETKSPYFGHLTDRMAHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKEMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGQLTPEELWHALESDFAGERGEDIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            50597..51265
                     /locus_tag="VCF75_000041"
     CDS             50597..51265
                     /locus_tag="VCF75_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP2545644.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYKGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     DSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            51322..52410
                     /locus_tag="VCF75_000042"
     CDS             51322..52410
                     /locus_tag="VCF75_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEP2545645.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGDSPILLCDDVVYGIVG
                     ERFESYLIDNSMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(52492..53001)
                     /locus_tag="VCF75_000043"
     CDS             complement(52492..53001)
                     /locus_tag="VCF75_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEP2545646.1"
                     /translation="MSTDHHLREVSYQLLAERGGSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGYSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            53364..54179
                     /gene="sdaB"
                     /locus_tag="VCF75_000044"
     CDS             53364..54179
                     /gene="sdaB"
                     /locus_tag="VCF75_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEP2545647.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(54420..55262)
                     /locus_tag="VCF75_000045"
     CDS             complement(54420..55262)
                     /locus_tag="VCF75_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP2545648.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            55761..55931
                     /locus_tag="VCF75_000046"
     CDS             55761..55931
                     /locus_tag="VCF75_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545649.1"
                     /translation="MDNPKKDKGLSLGVVDPITILITESVINKMRTVPYHLKKLSSTT
                     YCLLADLAYQSA"
     gene            56203..57399
                     /gene="speB"
                     /locus_tag="VCF75_000047"
     CDS             56203..57399
                     /gene="speB"
                     /locus_tag="VCF75_000047"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEP2545650.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDVNGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRGDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            57401..57706
                     /locus_tag="VCF75_000048"
     CDS             57401..57706
                     /locus_tag="VCF75_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEP2545651.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMVEAYQKQINSLD"
     gene            58186..59115
                     /gene="prsA"
                     /locus_tag="VCF75_000049"
     CDS             58186..59115
                     /gene="prsA"
                     /locus_tag="VCF75_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP2545652.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(59386..59622)
                     /locus_tag="VCF75_000050"
     CDS             complement(59386..59622)
                     /locus_tag="VCF75_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545653.1"
                     /translation="MIHRFFLTPLSTLWRRLRPKRYSKKVLADEPTGYQPPAAHGPCD
                     ETHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            60046..63072
                     /locus_tag="VCF75_000051"
     CDS             60046..63072
                     /locus_tag="VCF75_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP2545654.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSTGASSTETSAS
                     TNTSTASAGTSTSETAASGTGSEAAVVSSEGSQSSESGQASTQPQAQTLEQSAATSPS
                     SNSSISSSEDKAPKAASTKSSSATVASSSNGSNQGAGAEDAPQMMDVERYTVDREKTE
                     LNIKDGKTPKTRNSVDKDTKLIRNRDGKQRDIVDIKREVKDNGDGTLDVTLKVTPKEI
                     DKGADVMALLDVSKKMTDADFKNAKDKIKKLVTTLTSKSASNSDNDEHKHNSRNSVRL
                     MTFYREISNPIDISGKTDAELDKLLDDLRVKAKANYDWGVDLQGAIHKAREIFNKEKE
                     SKKRQHIVLFSQGESTFSYDIQKSEKEKSSNLSRINEKITSSNPLLPWPPIFDHTHQN
                     GDMINDVRALIAFASKLGIKNLSWIETSLSALSVGSNLLGSVFGGGGLGEYLTLKEYD
                     SQKLNEDQFDYTKRVGEGYHYHSFSNRESEDKMPLETEIKKALEAALPKFEENNWFTK
                     VLKYFGLKDKAEQAKLDVIMKVIKSVFYKRQYHYYNHNLSAIAEAKMAQKEGITFYSV
                     DVTALNTARRVKRQTAVLKNTKEEERNKKFDEYLKKMSEGGNAFFNDVDKADKFKDTL
                     TKLTIKDEFDGKVSVDKTSYQSSSAINYSEASNSFWRTTKESLTWTISKEQLKKAFED
                     GKPLTFTYKLKVDNNKFKTALEENNKKKRTKRSTPTENENSVTEKIISNTTTYEINEQ
                     KVEGNKLGDVSLTYSKFKVPVPQIDGHVIEPQAPTLPKLPPVIEHGPNFEYEEETGYQ
                     LPLKHGSNAPDTQVTIEEDTVPQRPDILVGGQSGPVDITEDTQPGMSGSNDATVVEED
                     TAPKRPDVLVGGQSEPIDITEDTQPSVSGSNDATVVEEDTVPQRPDILVGGQSDPIDI
                     TEDTQPGMSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPKEQPSLSLPQAPVYKAAHH
                     LPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
     gene            63252..65213
                     /locus_tag="VCF75_000052"
     CDS             63252..65213
                     /locus_tag="VCF75_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP2545655.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAISNRLEYQEQYKRVKEE
                     LDALKEKSPKLGERSLYFRLVNRFLGWFGVVWDPLAIKETDHLLDLIGQVNYLVSNTE
                     FQAPEYQKAKNKLDLAVAGYDLYLAREKKRGDAPDYQVTQLTLGQLETIKTKIQEALK
                     TFAQEVKTIQQKTPDLFPHAYVLNAKTSAIDGKAQPFLVNGYYDVFYKQFLFTNKNDG
                     YNYNKEADNLTSYQNLIKELLDLKAEIDRLTKDDPKNDRLETLMWQSKFTLSDFRRTS
                     RLSAYYTQGDYVKTVVLSEEAPNLRTAKEKVKTLKGILHDYYVETNKPEKAKEYRVED
                     ETLPSQPEAPVTPEAPAPSPVPGQKPAEAERSSQATEPANPSKENSSTDASQGSHDSE
                     NPATDSPSQPQAPDQGNHQSQVPNDKPQTDKTDTPNVPAPPQNTPNAPAIPQDTPKVP
                     EAGGQSGPAGNAEEKAPDSGPKASDQSSSKESPSVGEDTTPNQPDVLVGGQSEPIDIT
                     EDTITDAPPTVSGHNASTQPQSVVEDTAPQRPDVLVGGQSEPIDITQDTQPGMSGSND
                     ATVINEDTKPKRVFHFDNKEPQASEKAAEQKLAPHDSHTTPQASDDTAAPHLPASGDK
                     PEKAFALAALTMVAAAGLLSKKRRNTDKN"
     gene            65355..65651
                     /locus_tag="VCF75_000053"
     CDS             65355..65651
                     /locus_tag="VCF75_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEP2545656.1"
                     /translation="MTKQDQLIVEKMEQTYETFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            65800..66084
                     /locus_tag="VCF75_000054"
     CDS             65800..66084
                     /locus_tag="VCF75_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEP2545657.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            66508..67776
                     /locus_tag="VCF75_000055"
     CDS             66508..67776
                     /locus_tag="VCF75_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP2545658.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATAATEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            67763..68437
                     /locus_tag="VCF75_000056"
     CDS             67763..68437
                     /locus_tag="VCF75_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2545659.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            68450..69667
                     /locus_tag="VCF75_000057"
     CDS             68450..69667
                     /locus_tag="VCF75_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2545660.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            69761..70414
                     /locus_tag="VCF75_000058"
     CDS             69761..70414
                     /locus_tag="VCF75_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2545661.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            70411..71799
                     /locus_tag="VCF75_000059"
     CDS             70411..71799
                     /locus_tag="VCF75_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP2545662.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            71901..73529
                     /locus_tag="VCF75_000060"
     CDS             71901..73529
                     /locus_tag="VCF75_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP2545663.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKSSHTHPTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATEKTPEPNRDAPKPIQPPLAAAAPVFAPWRESDKDLSKLKPSSRSSAAYV
                     RHWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVR
                     AIVAIAMAESSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIAN
                     KNQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMTKLDQWIDDHGSTP
                     EIPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVD
                     RYMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILIS
                     ESNVMGLGTISYRTFTAEQASLLTYVVGDKLPRP"
     gene            73607..73858
                     /locus_tag="VCF75_000061"
     CDS             73607..73858
                     /locus_tag="VCF75_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545664.1"
                     /translation="MIKKVTTPSQKIKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
     gene            74534..76126
                     /locus_tag="VCF75_000062"
     CDS             74534..76126
                     /locus_tag="VCF75_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2545665.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKDCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPTSGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQRDEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIE
                     KQYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSP
                     SKSHSDKEVQKPDNSVKQATSS"
     gene            76313..77590
                     /gene="mrp"
                     /locus_tag="VCF75_000063"
     CDS             76313..77590
                     /gene="mrp"
                     /locus_tag="VCF75_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-related protein Mrp"
                     /protein_id="HEP2545666.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLANTTDVKAETV
                     GRFSDEQVRKAREKAIEDVFDGYTGARSVYQSGNLPNRLTPTKLSKLMQQMYKETLQK
                     KEELDTLSKALTHTIEKKIESENAYKKELGQLKAAAEAEAQKALDALNNKNKQISDLT
                     NENAQLKEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAKNAEIEALKQQDASKTEE
                     IAKLQSEAATLENLLGSAKRELTELQAKLDTATAEKAKLESQVTTLENLLGSAKRELT
                     DLQAKLDAANAEKEKLQSQAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKAL
                     KEQLAKQAEELAKLKADKASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLP
                     STGEETTNPFFTAAALTVIASAGVLALKRKEEN"
     gene            77815..78792
                     /locus_tag="VCF75_000064"
     CDS             77815..78792
                     /locus_tag="VCF75_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP2545667.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTTVKADSD
                     NINRSVSVKDNEKELHNKIADLEEERGEHLDKIDELKEELKAKEKSSENVERHYLRKL
                     DQEYKEQQERQKNLEELERQSQREVEKRYQEQLQKQQQLETEKQISEASRKSLSRDLE
                     ASRAAKKDLEAEHQKLKEEKQISDASRQGLSRDLEASREAKKKVEADLAEANSKLQAL
                     EKLNKELEEGKKLSEKEKAELQAKLEAEAKALKEQLAKQAEELAKLKGNQTPNAKVAP
                     QANRSRSAMTQQKRTLPSTGETANPFFTAAAATVMVSAGMLALKRKEEN"
     gene            79002..80108
                     /gene="enn"
                     /locus_tag="VCF75_000065"
     CDS             79002..80108
                     /gene="enn"
                     /locus_tag="VCF75_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-related protein Enn"
                     /protein_id="HEP2545668.1"
                     /translation="MARQQTKKNYSLRKLKTGTASVAVALTVLGAGFANQTEVRAEGV
                     KATTNLPEEAKYAALKDENTGLRGDQTKLVKKLEEEQEKSKNLEKQKQELENQALNFQ
                     DVIETQEKEKEDLKTTLAKATKENEISEASRKGLSRDLEASRAAKKELEAKHQKLEAE
                     NKKLTEANQVSEASRKGLSNDLEASRAAKKELEAKYQKLETDHQALEAKHQKLETDYQ
                     VSETSRKGLSRDLEASREANKKVTSELTQAKAQLAALEESKKLSEKEKAELQAKLDAQ
                     GKALKEQLAKQTEELAKLRAEKAAGSKTPATKPANKERSGRAAQTATRPSQNKGMRSQ
                     LPSTGEAANPFFTAAAATVMVSAGMLALKRKEEN"
     gene            80443..83991
                     /gene="scpA/B"
                     /locus_tag="VCF75_000066"
     CDS             80443..83991
                     /gene="scpA/B"
                     /locus_tag="VCF75_000066"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEP2545669.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VETPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPVTSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTADKQDKEMPVLSTNRFEPNKAYDYAYANRGMKEDDFKDVKGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PKKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDTFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSGQAPDKKPEAKPEQDGSGQTPDKKPEAKPE
                     QDGSGQTPDKKPETKPEKDSSGQTPGKTPQKGQPSRTLEKRSSKRALATKASTRDQLP
                     TTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED"
     gene            84088..85323
                     /locus_tag="VCF75_000067"
                     /pseudo
     CDS             84088..85323
                     /locus_tag="VCF75_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            85575..86495
                     /gene="lmb"
                     /locus_tag="VCF75_000068"
     CDS             85575..86495
                     /gene="lmb"
                     /locus_tag="VCF75_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEP2545670.1"
                     /translation="MKKGFFLMAMAVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            86508..88979
                     /locus_tag="VCF75_000069"
     CDS             86508..88979
                     /locus_tag="VCF75_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEP2545671.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGPATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMSKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQP
                     EGVQFYNKNGELVTYDIKTLQQINP"
     gene            89148..89348
                     /locus_tag="VCF75_000070"
     CDS             89148..89348
                     /locus_tag="VCF75_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP2545672.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(89428..90054)
                     /locus_tag="VCF75_000071"
     CDS             complement(89428..90054)
                     /locus_tag="VCF75_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2545673.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHDFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(90038..90841)
                     /locus_tag="VCF75_000072"
     CDS             complement(90038..90841)
                     /locus_tag="VCF75_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2545674.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(90853..91674)
                     /locus_tag="VCF75_000073"
     CDS             complement(90853..91674)
                     /locus_tag="VCF75_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2545675.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(91671..92648)
                     /locus_tag="VCF75_000074"
     CDS             complement(91671..92648)
                     /locus_tag="VCF75_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2545676.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVIRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNFIADILNSIINPQLRRKV"
     gene            complement(92761..94251)
                     /locus_tag="VCF75_000075"
     CDS             complement(92761..94251)
                     /locus_tag="VCF75_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP2545677.1"
                     /translation="MGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKT
                     YHLSEDGLTWSFDLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGK
                     NQVNIHLTEAHSTFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVR
                     NPYWHGKKPYFKKWTWVLLDENTALAALESGDVDMIYATPELADKKVKGTRLLDIPSN
                     DVRGLSLPYVKKGVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKP
                     AYSIIDKTPFWNPKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTN
                     DQLRANLAVEVAEQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHH
                     PSLAGKGWTNITFYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPN
                     VWLVSLNHTYIGDKRINVGKQGIHSHGHDWSLLTNIAEWTWDESTK"
BASE COUNT        30235 a        18634 c        19616 g        25900 t
ORIGIN      
        1 catttatcag ccctatcttg tttctgttct ctctaatagt ctataatggg ttataagtta
       61 caaaaagata ctaagaggag ctgctgccta tgctgaaaaa aagaccggct tgtcccgtag
      121 aaacgacctt atcggtaata aatagaaatt gttaatatta agagacttgc taaaggggac
      181 cttgcgtttt gggcagttga agtcctcaat aggttcggtc agtcaaaagg tccttactgc
      241 ccaattaagg gctatggaag cagacggatt ggttcatcgg gaagtgtatg cagaagtgcc
      301 accttgggtg gagtattcct taacagaaac tgggttgagc ttagctcctg ttattgaagc
      361 gatgtcggat tggggccaaa cttaccaaga aaaacatgcc gatttatttg aagaaggcta
      421 gaggaaagag ttgcccgagc atactgagac cgagagaagc tgagagtttt taaaaaatga
      481 tattatcaaa ttctttacag tttgataaaa ttactttttt tctacaaaaa gacttgcgct
      541 catctcacga atatgataat ataatatcaa gtatgtttga gaaaacagaa gctcaggtca
      601 agtaagtgca cacttacaat aacacagttg ataatgggga aactcagagt caagaaaaag
      661 aaaagcaagc cttgcatttt gcaaggcttt tttctgtctt tattttggtc tgcgccgagc
      721 aaaatctaaa aatttaaatt tttccagttt atggcgactc tcggtgaatt ggaattggtt
      781 attattggaa aggtagactt tggatttgac ggaaacaaca tgttggtctg atccaaggtc
      841 aagtaaaatt ttatcacgat cggttatttg gtcaatggta acctctttga gggcaaagtc
      901 aatggcgaga tggagttcct tttccaaata ttgataaata gaatgctctg cgatttccct
      961 tgtcattatt ggcaccaaag ttttactaag gtaatcaatg tctaagacgg aagccacacc
     1021 ctcaacaacg cgttggcgcg taacacgcca gaccaagctg tttttgctaa aaccggtcaa
     1081 ttttgaaagt gtctcatcca caatcagttt atcaatggca atgacgttgg tcttagaatc
     1141 tagatttgag taagagacga gttcctgata actagttagt tctgagatag ggaacatgat
     1201 ctgatgatgt ctaatcactt gggtgccacg tccttgtttt ttgaggataa gacctgcctt
     1261 tgtgagaagt gagagagcct tgcggacggt atctcggcta gcttggtatt gttgactcaa
     1321 ttctatttca gtaggtaaaa aatccccttc tttgtaaaag tctttgttta tttttgtttc
     1381 gaggtcttta taaatacgtt catactttgt catagggtaa attacctttc cacttgtcta
     1441 tttttttaca gacaacacct tttattttac caaaataatg gcttttttgg cacaaacaac
     1501 ttgcagacaa atttgcaatc gattacaata aaggtgtcaa tcaggttcta tctaagaatt
     1561 tgaatatttc attttgacgt tgcttgttaa aagcaactag aacaaaggac taggggatgt
     1621 cacatgggaa aatttgaaca ggatgctaaa agtcttctaa ctgctattgg cggtaaagaa
     1681 aacatcaagg ctgtcacaca ctgtgcaacg cgtatgcgtt ttgttttgaa tgataataat
     1741 aaggcaaatg tcaaagagat tgaaaaaatc tctgtagtta aagggacatt taccaatgct
     1801 gggcagtttc aggtaatcat tggtaatgat gttccagttt tttataatga ctttacagct
     1861 gtttctggta ttgaaggggt gtctaaagaa gctgccaaat cagcagctaa aagtaatcaa
     1921 aatgccttac aacgggtgat gaccatgttg gctgagattt tcacacctat tattccggcg
     1981 attattgttg gggggcttat tttaggtttc cgtaatattt tggagagtgt gccttttgaa
     2041 tttcttgggc agcaggtcga aaaagggaaa ttagtttttg atgcagctgg ggatcctgtt
     2101 tggaatacga ttgtgagggt atctcctttc tggtcagggg ttaaccattt cttgtggtta
     2161 ccaggggaag ctattttcca cttcttacca gttgggatta cttggtctgt gacgcgtaag
     2221 atgggaacca ctcaaatttt agggattgtc cttggtatct gtttggtgtc gccacaatta
     2281 ttgaatgcct atgcggtggc aggaacgcct gctactgaga ttgccaaaaa ctgggtttgg
     2341 gattttggtt tctttactat taatcgtatt gggtatcagg cacaggttat tccagccctt
     2401 ttagctggtc tgtcccttgc ttatcttgaa attttctggc gtaaacggat tccagaagtg
     2461 gtttcaatga tttttgtgcc attcctttct ttgattccag ctttgatttt agcgcatacg
     2521 gtattggggc caatcggttg gactattggt aaagggattt cctttgttgt gttagctgga
     2581 ttgactggtc ctgttaaatg gctattcagt gctatctttg gtgccttgta tgctccgcta
     2641 gttattactg gtttacatca catgacaaat gccattgata cccaattaat tgctgatact
     2701 gcaactcgta caactggttt gtggccaatg attgctcttt caaatatcgc tcaagggtca
     2761 gccgtttttg cttactattt aatgaatcgt catgaagaac gtgaggctga aatatcgctt
     2821 cctgcagcaa tttctgctta ccttggggta actgagcctg ctttatttgg ggttaatgtt
     2881 aaatacgttt atccctttgt agccggaatg attggctcag gtattgcggg tctcttatca
     2941 acaaccttta atgttcaggc aaatgctatt ggtgttggtg gcttaccagg tttcatggct
     3001 atcaatgtga agtacatgat tccattcttc atctgtatgg cagtagccat tgtggtaccg
     3061 atgtttttaa ccttcttttt ccgtaaatca catatcatga ctaagacaga agatgaggct
     3121 aaactacctg agacacccgt ttcggaggct cctgcagcaa ctgctccaca taagactatg
     3181 caagggacag ttatcacttt aacaagccct ttaacgggtg aagttaaagc gttgtctgaa
     3241 gctgttgatc ctgtctttgc acagggagtt atgggccaag gtgctcttct tcaaccgaca
     3301 gaaggggtgt tagtagcgcc ttgtgatgct gaagtatcgg tcttgttccc aactaaacac
     3361 gctatttgtt tggtgacgac tgaaggtttg gaattattga tgcatattgg catggatacg
     3421 gttaacttag atggtcaagg atttgaagct ttggtgaagc aaggtgatca ggttaaggct
     3481 ggacaaacat tgattcaatt tgatatagca gctatttctg aagctggata cgccactgaa
     3541 acgcctcttg tggtgactaa tcaagatgct tttacggtaa ctgttgaagg taacttaccg
     3601 catcagatta aggctaatga taagttagta gtagcgatga aaaagtaggt ttactaggga
     3661 gaaaacgttc aggaactgag atcagttcct gaaactattt tagggaggaa aactcatgac
     3721 aattgataaa aagaaagtcg tctatcaaat ttacccaaaa tcctataagg acactactgg
     3781 aaatggtgtg ggagacttgc gagggatcat tgataaattg ccttacttac aagaactagg
     3841 aatagatatg atttggttga accctttcta ccctagtcca caacgagaca atggctatga
     3901 tgtttcagat tatacggcgg tcaaccctga ttttgggacg atggctgatt ttgaagattt
     3961 ggtaaaagct gctaaggagc atcagattga gttaatgttg gatatggttt tgaatcactg
     4021 ttccacagac cacgagtggt tccaaaaagc tttagcagga gacccttatt atcaggattt
     4081 ctttatcttg agagatcagc cgactgattg ggtttccaaa tttggtggga atgcttgggc
     4141 gccttttgga gatacaggca agtactactt acacttgttt gatgtgacac aggctgactt
     4201 gaattggcgg aacccacatg ttcgtgagga attggctaaa gtggttaatt tttggcgaga
     4261 taaaggagtg aagggattcc ggtttgatgt gattaacctg attgggaaag atgaagagct
     4321 ggtggattgt ccggtcaatg atggtaagcc agcttatacg gatcgtccta ttactcacac
     4381 ttatcttcat gatctcaatc aagccagttt tggtcaggat gattcgttta tgacagtagg
     4441 ggaaatgtct gccacgacta ttgacaactg tcttttatac acggctcccg aacgtgagga
     4501 gctatccatg gcttttaatt tccaccatct aaaagttgat tatgagaatg gtcagaaatg
     4561 gactattatg gcttttgatt ttgcagcgct gcgagactta ttccatgctt ggggtgaagg
     4621 catgggtcag ggcaatgggt ggaatgcctt gttctacaat aaccatgatc aaccacgtgc
     4681 cctgaatcgt tttattgatg taacacattt ccgaaacgaa ggtgcgacga tgttagcggc
     4741 ttccatccat ttgtcacgag gaacgcctta catttatatg ggtgaggaga ttggcatgct
     4801 tgatccagat tttgatagta tggatgatta tgtggatgtg gaaagtctca atgcttactc
     4861 aagcttatta gtctcaggta aaagtgcgaa agaagccttt gccattatca aggctaagtc
     4921 aagggacaat gccagaacac caatgcaatg ggatgctagt gaacatgctg gctttacgac
     4981 tggtaagcct tggttagagg ttggcaaatc ttatcgagac atcaatgtcg aaacagaaaa
     5041 agaaggacgt atttttcctt tctaccaacg cttgattgct ttgcgtaagg aactccctat
     5101 tattgctgaa ggggactatc gggctgcttt taaagatagt caggctgtct acgcctttga
     5161 acgccattta ggtgaccagt gtttgcttgt tctcaatcat ttctatgctg atgaggtcga
     5221 actggaatta cctccacgtt atcaacatgg acaggtctta atcagcaact atgagaaagt
     5281 ttctatttgt gaaaaagtga tactgaaacc ttatcagaca cttgctatct tagctgaaaa
     5341 ctaactaaaa gcctgaggtt aggcctcagg cttttggtga ttgtcacgaa aaaagaagtt
     5401 ttctcatata tttattttta aactcttatt ttttctaaat aaaaacaatt gagataagtt
     5461 attttagcta aattttggta caatggaaaa gattataagt agagtttaag ataaagattc
     5521 tacaaattta aaatgatata aggagatacg aatgacaact tatcaagatg atttttacca
     5581 ggctgtgaat ggaaagtggg ctgaaacagc agttattcca gacgacaagc ctcgaacagg
     5641 tggattttca gacttagcag atgaaattga ggcattaatg ctagacacta cagatgcttg
     5701 gttagcaggg gatcatgtcc ctgatgatgc aattttagca aactttgtga aattccatcg
     5761 tttggtggct gactacgcta aacgtgatga agttggagta agtccaattc ttcctttaat
     5821 tgcagaatat cagtccttaa aatctttttc agaatttgtt gctaatatag cgaaatatga
     5881 attggcaggt ttaccaaatg aatttccctt tagtgtggca ccagacttta tgaatgctca
     5941 gctgaatgtg ctttgggcag aagcaccaag cattcttttg ccaaatacaa catactacga
     6001 agaaggcaac gaaaaagctg aggaattgcg tggtatttgg cgtcagtcgc aagaaaagct
     6061 tttacctcaa tttggttttt caacagaaga aatcaaggat cttttggata aggtaattga
     6121 gctagataag caattggcca agtatgtctt gtctcgtgag gaaggctcag aatatgccaa
     6181 attataccat ccttatgtgt gggctgattt taaaaaacta gctccagagc ttcctttgga
     6241 tagcattttt gaaaagattt taggccaagt acctgacaag gttattgttc ctgaggaacg
     6301 tttttggaca gaatttgcag ccacttatta ctctgaagcc aattgggatt tactcaaggc
     6361 taatttgatt gtggacgcgg ctaatgctta caatgcttac ttgacggatg atattcgtgt
     6421 ggaatcaggc gcttattcgc gcgctctttc aggaacacct caagctatgg acaaacaaaa
     6481 agcagccttt tatttggcgc aaggaccgtt tagccaagcg cttggcttgt ggtatgctgg
     6541 tcaaaaattc tccccagaag ctaaggctga tgtggaaagt aaggtagcac gcatgattga
     6601 ggtttataag tctcgtctag aaacggctga ttggttagca ccagccacac gtgaaaaagc
     6661 cattactaaa ttaaatgtta tcacgccaca tattggttac ccagaaaaat taccagaaac
     6721 ctattctaaa aaagtgattg atgagagctt gtcattggtt gaaaatgcac agaacttggc
     6781 taaaatcacg attgctcaca cttggagtaa atggagcaag ccagttgacc gtagtgagtg
     6841 gcatatgcca gcccacttag tcaatgctta ttatgatcct cagcaaaatc agattgtctt
     6901 tcctgcggca atcttacaag agccatttta ttctttagat cagagttctt cagccaatta
     6961 tggaggaatt ggtgcagtca ttgcgcatga gatttcacat gcctttgaca ccaatggtgc
     7021 ttcctttgat gaacatggta gcctaaatga ttggtggaca caagaagact atgctgcctt
     7081 taaagagcgt acagataaga ttgtggcgca gtttgacggc ttagagtcac atggtgctaa
     7141 gatcaatggt aaattgacag tttcagaaaa cgtcgctgat ctaggtgggg tagcttgtgc
     7201 tttagaagcg gcacaatcag aagaagactt ctcagcccgt gacttcttta ttaactttgc
     7261 gacgatttgg cgtatgaagg cccgtgagga atacatgcaa atgctagcta gcattgatgt
     7321 tcatgccccg ggtgaactgc gaacaaatgt caccttaacc aactttgatg ctttccatga
     7381 gacctttgat attaaagaag gcgatgctat gtggagagct ccgaaagacc gtgtgattat
     7441 ttggtaatta ggtagttgat aaaccttcaa atgcttgtga ttataaaaat gactgattct
     7501 gtgaaacaca ggatcagttt tttgcttcct tattggtgtc cgttttgaca caacaaaaag
     7561 acaaccctca ggaaagaagg tcgccttttt tagacttatc agaaataagt gattagttgt
     7621 taagggcagc agccatagta gctgcaactt ctgattcaaa gtcgttagct tttttctcga
     7681 taccttcacc aacttcaaaa cgtgcaaaag caatcgcttt agcgtttact gagtcaaggt
     7741 aagcttcaac agttttgctg tcgtccatga tgtaaacttg tgcaagaaga gtgtaagctt
     7801 ggtcaacttt agtgttgtca agcatgaagc ggtccatttt acctgggatg attttgtccc
     7861 agatcttttc tggtttgcct tcagcagcaa gttcagcttt gatgtctgct tcagcagcag
     7921 taatcacgtc gtctgataat tgagcttttg aaccgtattt caagaatgga agtgctggtt
     7981 tgtcaaccat tgcacgcgat tcgttgtcaa gttcgatagc gtggttcaat tgagcaagtt
     8041 catctttgat gaattgtgca tcaagctcag tgtatgaaag aacagttggt ttcattgcag
     8101 cgatatgcat tgatacttgt ttagcaagag catcgtcgcc gccttcgaca actgagataa
     8161 ccccgatacg gccgccatta tgttggtaag caccgaagtg ttgctcatct gttttttcaa
     8221 tcaaagcaaa acgacggaat gagatttttt ctccgatggt agcagttgca ttaacgtaag
     8281 cttcagcaag agtttcacct gaaggcataa caagtgcaag tgcttcatcg ttgttagctg
     8341 gtttgccttc agcgataact ttagcagttg cgtttaccaa ttcaacgaat tgagcatttt
     8401 tagcaacaaa gtctgtttca gcgttaactt caacaacagc agcaacgtta ccatgaacgt
     8461 aaacgcctgt taaaccttca gcggcaacac ggtcagcttt tttagctgct ttagccattc
     8521 ctttttcacg aagaagttca acggctttgt ccatgtcacc atctgtttca acaagtgctt
     8581 ttttagcgtc cataacgcca gcaccagatt tttcacgtaa ttcttttaca agcttagctg
     8641 taatttctgc catgtgtagt atcctccaaa tgatatttgt tttggtttca aaaaacgaga
     8701 cagagcggtt tgctttctgc cccgtttggt tttcgattgt taaagacaaa caaagtttgc
     8761 cataagagtt aggaactctg tgctaattaa gcgttgtcgc cttcgacaac ttcaacgatt
     8821 tcttcgattg aatctgcttg agtgtcagct tcaaaagcaa cgtctgcatc ttcaccttga
     8881 cggccttcga taatagcatc agctaattta gcagtgatta atttaacggc gcggatagcg
     8941 tcatcgttag ctggaatgat gatatcgata tcatctggat cagcgtttgt atcaaccatc
     9001 gctacgactg ggataccaag ttttttagct tctttaacag caatttgttc tttatgtggg
     9061 tcaacaacat acatcacgtc tgggatacga ggcatatctt cgataccgcc caagaatttt
     9121 tcaagacgag cgcgttgttt gttaagaagt gcaacttctt tcttaggaag aacgtcaaag
     9181 gttccttctt cttccatacg tttgatttct ttcaaacgag cgatacgttt ttggatagtt
     9241 ccccagttgg taagcgttcc acccaaccaa cggtggttga tgaagtattg gcctgcacgc
     9301 gttgcttcgt cagcaactgc ttcagcagct tgttttttag taccaacgaa caagatgaca
     9361 gcatcgtttg ctgcagcatc acgaacaaat tcgtaagctt ggtcagctaa ttttacagtt
     9421 tgttgaagat cgataacgtg gataccgtta cgctctgtga agatgtattt agccatctta
     9481 gggttccagc gacgagtttg gtgaccaaag tgaacaccag cctcaagaag ttgtttcatt
     9541 gaaattactg ccatgagtag tttctccttt ataaaatgtt ttttcctctt ccagacttcg
     9601 acttgcaggc taacctagcg gcaacaagcc cacaattggt ctagaatgag tatttgccgt
     9661 ctacacgacc ctactatatt atcaaaaaaa gaggttaaaa gcaaggagaa atgctatgaa
     9721 ttcccttatt ttcgagatga ctagcttctt tgtttattgg taagtgttaa gacaagcttt
     9781 tgctcatttt gggtgacact tgccaaatcg ttttatttaa gatatagtga acttgactaa
     9841 tttttggagg cgttttcatg acgaagggta ttcgatttca attgcttggc agtccccaca
     9901 tttttcttga tgacaaagaa caattttttg cctttgccaa agcaaatgct ctcctttatt
     9961 acttagtggt taatggatct gttagccgcg aggtggcggc gagtctactt tgggaaaata
    10021 agaacacgca aacagcgaaa aagaatttac gaaacgctat ctaccaggtc aataaagtct
    10081 tgcaagctga tgtgattatt tgtcccaatc gcaacctgct ggttttgaat aaaacacttg
    10141 atatcaaaac agatattaac ctttttctgg ctgatccgct agctcactta gaactctacc
    10201 aaggggagtt tttgcagggc ttttacctta aaagtggtga ggaatttgat ctttgggttt
    10261 caaaaatgcg tatgcagtat gaacaggtat acctaaaagc gtgttaccaa aaaattgaag
    10321 aaaagctctc tttagatgcc attgaagatg tcgaggaaca tttaaaacag cttattgaac
    10381 gtgatgaatt tgaagagaaa aattatcagc tcttgatgag gctttatcag caggggaatt
    10441 gtcctggtaa ggtcatcgaa acttattatc agttggctaa tgtgttggat aaggagctag
    10501 ggattcagcc aagtttgcaa agccaacaga tttatcagga agtggtcgct aaagatcgta
    10561 atgaacgcaa aatcaagcat tttttgcgca acagtaatca ttttttgggg cggattgatg
    10621 agattaagca gctggaaaac tattttgcca actgtttggc ctgccaagag gtaggagctc
    10681 tgctcttaat tggtgataca gggattggga aacgaaccct agccagacag gtcttagcca
    10741 atcaaaccca aacgtttcaa attgtcacag ctaaatgttt tcgtgaagag gctatggatt
    10801 ccttattgcc ttggcgtaat atcttagatg gcctgggaga tttggtgatt caaaaccgct
    10861 tattgaccac caaagcttgg aaggctgctc ttaaacgctg ttttcctgtg gcaaccattt
    10921 ttcaagaaga taacaaccaa ccctttatca aggaccacac cagcttgttg gtttccttta
    10981 ttgttgatat tttacaacat ttggcagaaa tcaaggcgct gataatcctt attgaggatt
    11041 gtcattggat ggatgaggac agtttgacct tgttgcaacg ggtcatgaat caattggtcc
    11101 actatccgat tgcttttgtc ttgaccaagc atctaggaac gactcctgag ctcggtcttt
    11161 gtttgaatgc tttgatgagt caggggtgtt tagaaagcat ttgtttggag ccttttaatc
    11221 ggcaagaaag tttggcttat attaacagtc aactgggtag tcagccagtg acggaagaag
    11281 aaatggagca cctctatcaa gccagccaag gcaccccttt ctttttgtca gaatacactc
    11341 aggccctact gcgtcacgaa aaatttgtgc ctttaacgcc tgccattaag gctaagttgg
    11401 gtcttaaact agctaatcta agtagtcgtg atgacgtatt gttaaactat ttgtcttgtt
    11461 gtcggaggcc tatccctcta aataccctcg ctcaattgat gttactgcct ttagaagaag
    11521 tgatcgagat ggtggataat ctggggcact actatatttt ggtggaagaa agcgtaggag
    11581 aggaggtgtt gatttcattt cgtcagcgaa ttattcaact ctatagttat gaccgtttat
    11641 ctttgtcaaa aaggcgttta ttgcacggac aaattgccaa acggttagaa gatttactgc
    11701 ctattttgac accaagccct cacttattag atgacattgc ttaccattat caggaatcac
    11761 ggcaggtgat taaggctttg gaatacaacc ttaactatct agatgctacc ttgccatttc
    11821 agcatgagct gtttcccatt tattcaaaga gtattggctc cttggaaaag tcagatcgtg
    11881 accatcaacg cttaatgcaa gagcagtttg ataagattcg gcaaagcatt gcggatttgg
    11941 aactgaccta tgataataac cgtgatttcc agcagttgct tattcgtttt tcttaccttg
    12001 aaggccgtta tgatattcga acagggaggt atcaagaagg gattaaacac attcaaaagg
    12061 tcatcgcttt ggcaaccgag ctaaaacagc cctccttctt gctagaaggc tatcgccagt
    12121 taattcatta ctgtatccaa gtggaaaaca agccagaaat gcgttattac actagtcttt
    12181 cattggaggc agcggttgct gctaatcatt ttgaggccat tgcgattagt ttgcggttaa
    12241 acggtcttta tcatttgatt attggggagt taaatgaggc agagcgtctg cttcaacagt
    12301 cgattgactt ctttaaggtg acgtcaggct tgcaagctaa ctatgcgatt cagattgctg
    12361 ctgccttaga ttatttgggg gagatcgccc agattcgcta tcagtttgaa aaggccgttg
    12421 cttaccaaaa acaagccatt gccttgaccg aaaacaagcc agctgagtta tcagtgagta
    12481 ttttttatat tggattaggg atttcctact tttatttagc tgattttgaa caagctgagc
    12541 aaatcttgag tttggctaag gaagctttgg tcaatcatag ttatccttgg aaagaaacgc
    12601 aacttgaaat ttacctggcc atgattcaat ggaaaaaggg taattatcag ccagccttga
    12661 ctctacttga ttacagagaa accttgatgt ctaggtatcg caatccgcga gataagggct
    12721 tggttttcta tttgatggct gtggtcaagt accaattgat acgtcaagga gctaccctaa
    12781 gtcagcagga aaaggaaatg gcagaccacc tgttgtcaga atcctttgag tattattatg
    12841 agattgctag tactaacctc aacccttacc gagattgtca tttggtgagt gagttgaacg
    12901 atttgcgtca acaattatct gctaaaagct aaggaaaacc ctctgattca catcagaggg
    12961 ttttagttat gttgtgccat aatctgtacg aggtaaaaga taaaggtggc tgctaaattg
    13021 gcggtgtggt tatcaatatc gtgtggtggt gaaacctcaa ccacatcaaa gccaactaat
    13081 tttccgctag ctgcaatgtg ttgcaaaacc aagacggcaa gttttgggtc aacaccgagg
    13141 gattggatag cactaacccc aggtgcagca ccaactgaga agcagtccat gtcaatggtc
    13201 agatagactc tttcttgccc ttctaagaaa cgatcaatgg tttggcaaac cttttgatag
    13261 cccatgcgat aaatatcttg cccagtcaaa aattgaatgc ccttggattt tgccacaaag
    13321 tcaaatagga aaaggttgtt attatgttct tgaatgccta aaacgaagta tttaaagagg
    13381 cgtttgtcag caaccgcatc gtcaaacatt tgtcgaaagc ctgtccctga attagggcct
    13441 gtttggtcgt agggtcttaa atcaaagtgg gcgtccatat tgatgacagc taggtcatca
    13501 cttgaggata ggctttggcg taggcctaag tagtggccgt aggcggtttc atggccacct
    13561 cctaagacga tgggtttgag attgaggtca cacatccgtt tgatggcttt gcttaaactg
    13621 ttttgcagtt gttccaaaga gcgattgggg ccgtcaatgt tacccacatc gtaaaccata
    13681 acttggtttc ctaaatgcca agggaatttg gcgagctggg tacgtatagc agctggactt
    13741 tcgaccgctc cgacacgacc attattgata taaactccct tatcactttt aaagccaatc
    13801 aaggcaaaat gtgtcccttc aaatggggtt agtgagctgt cgttaaggtc aaggaaagtc
    13861 atgaccatgc cccatttggc agtatataag tcatcgtcga tcccaccatg ataatagcta
    13921 gtggtggatg ggtagtaatc ttctagcatg cgcgtcatct cctttaggat tatgatagca
    13981 aaataaaact tacagtcaac actttcttgg cctttgtcac tgctttttag gaatagcaga
    14041 gcaataaaat cgtctgcttt tggtcccggt tagttagtct aaagaggcca tgacctctgc
    14101 cacagcccct ctagttaaag aagtacctta ttcactaaaa ttggatactc aactcaactg
    14161 cctgctcaac cgcttcgagg aagcttgggt cttcaatgac tgctgatgcc ttgtttagtt
    14221 catcgtagat ttcgatgtgt ttatcgtgtt cgataaagtt gacttccttg cggaagagat
    14281 cgtaggcaac cttagtccct ttacctagtt catgattttc agatttgaga tcaagagctt
    14341 ggcaggctgc catgatttca gtagccacga tgcggcgaga atttttaagg atttcaaagg
    14401 ccttgcgagc agcggtggtg cccatgctga caaagtcttc ttggttctca caagatggga
    14461 tagaatccac gctggctgga tgtgccaaaa ccttgttttc agaagcaaga gaggcacagg
    14521 cgtactgggt aatcataaag cctgaattga gacctgggta tttgacaagg aaggatggca
    14581 atttgctgag ctggctattg accaaacgtt ccacgcggcg ttcagaaaca tttccaattt
    14641 cagaaatggc gatgcctaag aagtcaaatg gctgtgccat tggttctccg tggaagttac
    14701 ctcctgaaat aacgtgacca tctttacaga taattggatt atctgtgaca gaattgattt
    14761 caatgtcaac tttggatttg acataggcga tactgtcttt gctagcccca tgaatttgag
    14821 gcatgcagcg caaggtgtaa gggtcttgca cacgagattg agtagctacg gttgtgtttt
    14881 ggctgccttc aatgaggttt cggatattgc gggctgttgc caactggccg ctttgtgggc
    14941 gaatggtgtg aaggttttct tcaaatggac tagtaatccc gttatgcact tcaagggata
    15001 gggcaccagc taggtctgac agtttcaata attgaatggc gtcataggtt gcaagggcac
    15061 cgattgcagt gagaacagtt gttccgttga taagggcgag tccttcttta gcggctaaag
    15121 aaatcttgtc aataccagct ttatcaagag cttcttgacc agaaagaagc tctcctttgt
    15181 agtaagcttt ccctagacca agcataggaa gcaccatgtg agcaagcggc gccaaatctc
    15241 ctgaagcccc tagagaccct ttttcaggaa tgtaaggatg aaccccttta ttgagcagtt
    15301 ccaacagctt ttcaatggtg gatagacgaa taccagaata ccctttgacg agagagttga
    15361 tacggatcag catgatagcg cgcacggcat cttctggcaa tgggtcacca aatccactag
    15421 catgggtacg aatcaaattt tcttggagtt gaactgtatc ttctggtgag atgctcacgt
    15481 tacaaaggga tccaaaaccg gtggtcaccc cgtaaaccac ccgtttttca ctgacaatgt
    15541 catcaacaat tttacgagag gcattcacag cttcaatagc actatcatca atgcgacagg
    15601 ctatgccttg gcgagcaatc gcaatcacgt cttcgatagt aaggctttcg ccatctaaat
    15661 taatcactct tgtcatagtt tttctcctag atttgttcct tagggggcat ttggtccatt
    15721 tggtggttac gtccgtgcat aaggtagtac acactacttg caatgatgac accaactgca
    15781 ccataaacaa agttcattgg gttgtcaccc caaatcataa ctaaactaac gataactgca
    15841 agaattggga taataggacc aaatggtact cggaagatca cattagcatc gggatcgtcc
    15901 ttccgtaatt tcataacggc tagtgctgtt ggaatgtatt ggaagaagcg gaaaataaca
    15961 ctgagttttg ctaagttttc aaatgagcca gtgagtagga gtacaatagc aattgctcct
    16021 gaaaccaaga tagcaacgag tggtgcgcca ttttgatttt gcttggcaat agcagctggt
    16081 aagagccctt catcagcaat ggcagcaccg taacgtggca ccatgatgga ttctcctatg
    16141 ttaagacctg taatggaaat taaagcaccg attgagacca tccaagcgcc agcagggcct
    16201 atcattttaa caaaggcatc ttgaacaggt gcattggtca tcatgatttg agagccaagc
    16261 atggcaatgg ttccaccgat aatcagcata tataagacag agacgatact gattgatcct
    16321 aaaagagcac gcggtacatt tttttcaggg tcacgcattt ccccagcgac aatagagagg
    16381 gtttcaaaac cgataaagcc atagaagata taaacagcag tattggagat agcccctaag
    16441 aggttagtcc ctggttccaa ctgaacaaat ggggtaaagt taggaagccc gtttttgatg
    16501 aagaaaaggg tacaggcaca aaaggcaacg attggaatta acttagcaat ggtggctgtg
    16561 atggtgacaa ttttagaggt tttcaaacca gcgatattca tcaaagatag taagatgatt
    16621 aacccaatgc taagtggaat gtgccaacct tcaaaggcag ggaaagtaat gataaacatt
    16681 ctggcaaacc cagctgccat ggctgcccaa gcgaagatgg taacggtcca accaaggaag
    16741 ccaacgttaa agccaataaa atcaccaaaa gctcgtttgg aatattggaa ggctccgccg
    16801 tttttcccaa aataaccaga gacttctgca aaacaaactg ccaacatgat ggtcaaaata
    16861 gctgttccaa acataacagc gatggatgca gggccaaggc ccttgtaaat ggccctaggt
    16921 aagagaaaaa taccagagcc gataacggca ttgatcccgt aaagagtggc accgctcaag
    16981 ctaaactttg cctgttctct ttcttgttcg ttcatgtgat ttgacgccat aataatgctc
    17041 tcctttttgt ttgcaaattg cgttcaaata taatggataa ggtcgctagg aaatgaggag
    17101 atagtcggtt taggagggtc cttcttgcac ctaacgatag tgatgagcta ttggttagcg
    17161 atgagaaata ggtagccttt tgtgatgatg tgtagtgatt tcattggaga agaataggca
    17221 tgagtagtgt gatgcctatc tttatcccac taagtcttat accataagcc tgcccaaaca
    17281 gctactggag tttgggcggg gcttgtttga ttggggcaaa cttttaggtc tgaaaggctg
    17341 gcctcctgct cgattaccaa caagtgattg gcttcaagct cttgattcgc ctgattggtc
    17401 acagaataag tcagtttgct tagggcataa agcagttggc agtccgcttt gggtgcctca
    17461 aatagtgtgt cagaccagac gggtaataat tgcccttggt aatgatcatc atacattaaa
    17521 ttgatgtctt ggcaggttcc acgactgacg attctggtat cgccttcaaa cgcatagggt
    17581 tcaaaaggag ctagggaaat ctctttagca tgactcaaat cacttaatgt gatgggcttg
    17641 tctaggctca ttaggatcct gtgatagcca tcaagtcggg taaaggttgt ctgtcgtagg
    17701 gctacagttg cagttgagag acgataggag aacgttcttt ctttgtagtc tcccttttca
    17761 ggataaagga acaattggtt ggtttcaccg ccagaccagt cagtttgagt gtaatctgct
    17821 ggcgttttga ccattacggt tgtcatctgt tctctccttg tttccaatta gaatagtcct
    17881 gtgatgttgc cctcctcgtc aatatcaatt gtttcgctgg caggtacttt tggaaggcca
    17941 ggcatggtca tgatagcacc tgtcagggca acgataaatc ctgctcctgc ggacacttta
    18001 aggttgctaa tagtcactat aaagtcagtt ggtgcgccaa gttttttggc atcgtctgag
    18061 aaggaatact gggttttggc catgcaaatt gggtagttgc caaagccgag gcgttctaag
    18121 tcagccagtt cacgtttggc tgcgggggtc aaggtgatgc ctttaccacc gtaaaccttg
    18181 gtaacgattt tagtcagttt ggtttcaatg ctatcgtctg catcgtaaac aaagcggaat
    18241 tgattgtctt gctcggctag tgtcacgact ttttcagcta attcacggcc accagctccg
    18301 ccatttgccc aaacatcaga aatcacaacg tcaacgccac gtttgtcgca ggcatcatag
    18361 acagcttgta attcagcatc ggtatcaagc gggaatttat tgatcgccac gacaactgga
    18421 aggccataga cgtcttggat attagccaaa tgtttgtcaa ggttaggtaa accatccaca
    18481 acggcctgaa cattttcagt agccaggtct gcttttggca cacccccatg cattttaagg
    18541 gcacgaatag tggctactag gacaacggct gctggacgaa ggccggacat gcggcatttg
    18601 atgtcaatga atttttcagc tccaaggtca gcaccaaatc cagcttcggt aacggcataa
    18661 tcaccatatt tcaaggctag tttggtggct aggacactat tacagccatg ggcgatatta
    18721 gcaaatgggc caccgtgaat gagggctggt gtgtgttcca gggtttgcac gagattagga
    18781 tgaatcgcat ccttgagcag tgcggctagg gcaccaccag ctttcaagtc tttagctgtt
    18841 acaggttcgc cttgatagtt gtaaccgatg atgatttttt caaggcgggc tttgaggtca
    18901 gaaatatttt cggataggca caggattgcc atgatttcag aggcgacggt aatgtcgtaa
    18961 ccatcctcac gagggacccc gttgaccttg ccttgcaagc cgtcaacaat atgacgaagc
    19021 tggcggtcat tcatatccac cacacgtttc caagtaatac gtctggaatc aatgcctagg
    19081 ctattgccat ggtgaatgtg gttatcaatc aaggcagcta ataagttgtt ggcgacaccg
    19141 atggcatgaa agtcccctgt aaagtgaagg ttgatgtctt ccattgggac aacttgcgcg
    19201 tggccaccgc cagcagctcc tcccttaacc ccaaaaacag gaccaagaga aggttcacga
    19261 agggcaataa ctgctttttt tccaatggca gaaagagcat ctaccaatcc aacggaagtg
    19321 gtggttttgc cttcaccagc tggtgttggt gagatagcag tgacaagaat gagtttgcca
    19381 tcaggtttgt cttttaaggc gaccagttga cgggcatcaa tttttgcctt gtacttgcca
    19441 tagagacaga gcgcttcctc gtcaatccct aattgattgg caaccttact gataggctcc
    19501 atagtgacag agttggctat ttcaatatct gataaaacca tagttactcc tttacttgtt
    19561 aaacaatatc gagaattttc gtgtagatgt catctgccaa gtgacagccc ttgtctaata
    19621 aggcttgtcc tttttggcga tagtcggtaa caaagtcttc atctttgatg ccagacagat
    19681 taatcaaaac attcaaccaa gcgctttgta aacctgcttt taagtttaaa gcagcaactc
    19741 ctaagtcgct agcagcattt gtattagact taccgacagc tgttgcagtg atctcaagtg
    19801 cttcgaccat gagggtcatc atctcaaaag gtgattgtgc agctgttttc aaggcctttt
    19861 gcatggcagt tcgtctagca gccttgtctt cgtcagtctc ttttggcata tcaaaaacgg
    19921 cagaaaccag gttgaaggct tcggtatctt tgtcaatggc tgcaagcaag ctagcttgta
    19981 gagcggtgct tttagcatgg atctctgtaa taatagcttg gtagtcagcg tattttttct
    20041 taccaagagt caattcacag accatcttgg ttaaggaaat gccattagcg ccagagagag
    20101 cagcagcaga accaccacct ggagcagggg catctgagcc aaggacttta gcgaaatctg
    20161 ttaagcttaa atcaactaaa cccatagaga acctcctaac ctagcaaatg gttttcaaga
    20221 atctgtttat ggtagtcaaa gtcctcaact tgtaagtagt attcagccac atcgattaag
    20281 gccttggctg gcgctaagcc gatgatttca gaaccaataa cattcacacc gtagcgacgc
    20341 gcttcaaatt tgatggtttc aaaagtacgg taaagagaac atttttcaaa gttaaccatg
    20401 ttcatggaaa cttgggcaat atgacggtct tctaacatga caccgatagc cttacaatat
    20461 ttgtagccac cacctgaacc gcggataatt ttagcaattt tatgagcaat gtcgatattg
    20521 tcagtatcca aattaacatt gaaggcaacc aatggcatgc gagcaccaac ggcagttacc
    20581 ccagcagttg ggtgaatctt acgatctccg tagtcaggtg cccaatcctc ttccaacagt
    20641 ttttctggca tgccttcgaa ttgtccttta cggactttgg ccaggttttg acgttctggg
    20701 cgtgtggcag agtcttcgta aaggaagatt gggattccaa gttcacggtt aatccgttct
    20761 gcgacttgtt tggaaatctc aacacattct gctgtggtga tgtctttgat tgggacgaac
    20821 gggcagacat cggtggcacc catacgaggg tgttcgccat gatgtttggt catgtcaatg
    20881 ttttcagaag cgtatttcac cagttggaaa gctgcttctt gaatagactg gtcatctcca
    20941 actaaggtaa acacgctccg gttgtggctc gcatcagaag agtagtcgag aagggtcact
    21001 ccaggaatac ttttggtagt cgctaccaag ccgtcgataa cagcctgatt ttggccttct
    21061 gagaagttgg gaatacattc aacaattttc gccatgttta atctcctttt atgtcattga
    21121 gttactcatt aagagaatag gttacctaat ttagataacg cttacatttt tagctagcag
    21181 ttattagtga aataattgat ctaccgctga agtcacaagg tcatcatcag ccaagtatgg
    21241 gatagtgatg tgatcagtac ctgcatggag acggttgtat tcaatagcgg tttcaatagc
    21301 gtgctcattg cgagcccaat tgcgacgagc aacaccaccc atggtgtccc aagcaatggc
    21361 agatttaata atctcatcaa tgcgttcgct gccatctaaa acaagaccga aaccaccatt
    21421 gatggcttta ccaataccag ttccgccacc gttatgaaga gccactaaac tcataccacg
    21481 cgcagcgtta ccagcgtaac attgaaccgc catatcacag gtcacattgg aaccgtcttt
    21541 aatgtttgag gtttcacgaa atggtgagtc ggtaccagac acgtcatggt ggtcacgacc
    21601 aatcatgaca ggaccaatct tgcctttgcg gaccaattca ttaaatttaa gggcgatagt
    21661 cacacgaccg atacagtctt ggtaaaggat ccgggcttgg gtaccaacta cgagttgatt
    21721 tttctcagca tcacgaatcc agttgtagtt atcacggtct tgatagcggc ggtcaggatc
    21781 gatagcttcc atggcagctt tgtcagtggc caccaagtct tcgtgtttac cactcaagca
    21841 gacccaacga aaaggtccat agccatagtc aaaaagcata ggtcccatga tgtcttcaac
    21901 ataagaaggc cagataaagc cgtctttgtc gttacgacca ttcttagaaa tttctgtaat
    21961 accagagtcg taaacagact tcataaaggc attaccgtag tcaaagaaat aggtgccatt
    22021 ttcagttaac gtcttaatag cttcaaaatg ccgtgccaag gtatcatcaa ccatttggtg
    22081 gaaggtatct ttatcttctg ccaagagccg ggttctttcg tcaaagctaa tacctactgg
    22141 gcagtagcca ccgtcataaa cgttgtgaca agaggtttgg tcagacaaaa gatcaacatg
    22201 aatctgcttg tcattgacgt actctaaaag atcaacgata ttaccatggt aagcaatcga
    22261 agtggactct ttagcatcta tggctttttg ggctaattgc agagcttctt cagggctctc
    22321 agcaatttgg gtaacccagc cttgcgaatg acgggtttca atccgagatt ggtcaacttc
    22381 agcaataatg gcaactgctt tagcaatttc agcagcctta ccttgtgccc cactcatgcc
    22441 tcctaaacca gatgagataa agagtttacc ggtcaaatca ccatcgtcag ccaccccaag
    22501 tttgagacga ccagcattta agagggtatt aaatgttcca tgaacgattc cttgtgggcc
    22561 aatatacatc caaccacctg cggtcatttg accatagttg gtgacaccca tctcttcagc
    22621 gatttcccag tctttcatgt tgtcgtattc acccaccaaa aggccattag taataatcac
    22681 acgaggcgct tcaggtttgg atttgaaaag accaactgga tgaccagact caaccaccaa
    22741 ggtttgttca tcagtcatga cttctaggta ttttttaatc aaacaatatt gcatccagtt
    22801 ggcacaaaca gaaccagttt ctccataagt gactagttcg taagggtaca aggcaatctc
    22861 aaaactcaag ttattgtcaa tcatgacctg catggccttg gcagctgtac aatttccctt
    22921 gtactcgtta atgggtttgc cgtaaatgcg gtcttttgga cgccatctgt aaccatagat
    22981 gcgtcccctt gttttgagtt cttccaaaaa ttctggaatg acttcttcgt ggaacttcgt
    23041 aggtacatag cgtagggcat ttttcaaggc gatttcggtc tgagcctggg tcaatcgaaa
    23101 acctctgtct ggtgctcttc ggataccttc tttgaagaca gtcttttccg gtaaaacatc
    23161 gtctaactta acagtcatag cggcagctat gtcagtttca ctgtaaaatg acatagaaaa
    23221 ctcctcctca actccttttt attgtcgaga ttagtctaac ttagtctagt ctataaaaag
    23281 tcaaccacgc tttgaaatgc gaaaaaagtc taaaaaaaaa tcaaaacctc attttttgac
    23341 taaaatttga cgcttaatca gtacacttga ggcatgacag ataaagtcag tcagaggtta
    23401 attgttgtta ccaaagttgc aaagatcgca actattgagt gggtgagggg ggactagtaa
    23461 tggctattaa tcagttgaca aacgatgtta aaaaaggagg tttttatggt tgctgatgtc
    23521 ttattaacac attttaatca actattttgt ctaaatgatc caggtcatcc tttaactggt
    23581 caagaaatga aaaaagccac tatcgttgaa gatggctata tagccatcaa agacggcctc
    23641 attgtggctc ttggttctgg tgaaccagat gctgagcttg tcggtccaca gactattatg
    23701 cgttcttata aaggtaaaat tgcaacgcct ggtattattg actgtcatac ccatttggtc
    23761 tatggtggca gtcgtgaaca tgaatttgcc aagaaattag caggggtgtc ttacttggat
    23821 attttagctc aaggtggtgg tattttaagc acggtgaggg ccactcggtc agccagcttt
    23881 gacaaccttt atcaaaaatc caagagattg ctagactaca tgttgcttca tggagtgact
    23941 actgtcgaag cgaaaagtgg ctacggcctt gattgggaaa cagaaaaacg ccaactggat
    24001 gttgttgctg ctttagaaaa agaccatcct attgatttgg tgtctacgtt tatggcagcc
    24061 catgcgattc ctgaagaata caaagggaat cctaaggctt atctcgatgt gattatcaag
    24121 gatatgcttc ctgttgttaa agaggaaaat ctggcagaat tttgtgatat tttctgtgaa
    24181 aaaaatgttt ttacagctga tgagtcccgc tatctgttaa gcaaagccaa agaaatgggc
    24241 ttcaagcttc gtattcatgc agatgaaatt gcgtctatcg gcggagttga tgtcgcagca
    24301 gagctcagcg cagttagtgc agagcatttg atgatgataa cagatgatgg tatcgctaag
    24361 ttgattggtg ctggtgttat tggtaatttg cttccagcca ccacctttag tttaatggaa
    24421 gacacttatg cccctgctcg caaaatgatc gatgccggca tggctatcac tctctcaaca
    24481 gatagtaatc caggcagttg cccgactgct aacatgcagt ttgtgatgca attaggttgt
    24541 tttatgttac gtttaacacc gattgaagtc ttaaatgctg ttaccattaa tgcggcctac
    24601 tctgtcaatc gtcaggagag ggttggtagt ttgacagttg gtaaagaagc agatattgcc
    24661 atctttgatg ccccaaatat tgattaccca ttttatttct ttgccaccaa cttgattcac
    24721 caagtctata aaaaaggaca actcacggtt gatcgaggtc gtattcttta aaaaatttca
    24781 aaaagaagct ttgagcgtta gctcaaaact tcttttaaat atgctgtttt attgacgaat
    24841 caagtagtca aaggcaccga tggcagcagt agcaccagac cccatggaaa taatgatttg
    24901 tttataagct gaatcagtac agtccccagc agcaaagatg ccgggaatat tggttgaacc
    24961 atgtttatcc acaatgattt ctccgcggtc ggttaggttg acaccactat ctttgagcca
    25021 agctgtgttt ggaataagac caatttgaac aaagacccct tcaaggtcaa ggtgtttgtc
    25081 ttcaccgcta tcacgttcag tgtagttgag accagtaaca tgatcgtcac caacaatgtc
    25141 tttagtagca acatttttga taatagtcat gttagcagta tcagctgcgc ggtcttgaag
    25201 cactttgtca gctttgagct caggcaagaa ttctaacacg taaacgtgtt tagcaagacc
    25261 agccaagtca agggccgctt ctaaaccgga gttaccacca ccgataacag caacatcttt
    25321 gccttcaaag agtgggccat cacaatgagg acagtaagtc acccctttat tacggaattc
    25381 atcttcacca ggaacattaa tgttacgcca tttggcccca agagctaaga tagcagtttt
    25441 ggcttgtaga accgcaccat tagctagagt aacctcgatg ttttcttttt tctcaataga
    25501 agtagctagt tgtgctttga taatatcaac atcataagac ttagtatggg cctccacttc
    25561 ggccattaat tttggtcctt cagtgtaaag ggtaccaatc atgttttcga tgccgaccgt
    25621 ttccatgacc tgaccaccaa aggtttcagc aagtaaacct gtttttagtc ctttacgagc
    25681 tgcatagata gcagcgctat tgcccgcagg gccaccacca ataacaagga catcatagag
    25741 gcctttatca gcaaaggctt cctcagaaag tgggccagcg atttgttcta agagttgctc
    25801 aatggtagcg cgaccagaag tgaattcttc cccgtctaaa aagacagtag ggacagacat
    25861 aatgcctttt gctttcactt catcttggaa catgccgcct tctaccatag tgtgtgaaat
    25921 cttgtcgttc aagacggaca taatgtttaa agcttgaaca acatctgggc agttatggca
    25981 ggttaagctg acataagttt caaaatgcaa cggacggtca atggccttga tgcgatcaat
    26041 aacatcttga tccacttttg gagcacgtcc agatacttgc aagagagcaa gaataaagga
    26101 cgtcaattcg tggcctaaag gaagaccagc aaagacaacc ccactaccgt gacctttttt
    26161 cgcaacctta aagcttggtt gtcgatctaa agtgatgttt tcaatagaaa tacgctcaga
    26221 catagcagct atttcttcca caaagtcttt gactttttga gactgctcat tgtcaccaag
    26281 ggaaacttgc aaaacaagat cagcttctaa cagggtgagg tattgggcaa gttgttcttt
    26341 aatatcagga cttaatgcca tagctttcct cctaaaattt attaaatttt accaactaaa
    26401 tcaagacttg gtgtcaaagt ttcagcgcct tctttccatt tagctggaca aacttcacct
    26461 ggatgtttac ggacgtattg ggcagcgtga attttatcaa tcaaggtgct agcatcacgt
    26521 ccaataccat cagcattaat ttccatcatt tggataatac catctgggtc aacgataaat
    26581 gttccacgtt gagcaagtcc gtcttcgcca agcacttcaa aggcttgtga aataaggtgt
    26641 gaagggtcac caatcatagg gtatgtgata gtgccaacca catctgaatc atcatgccaa
    26701 gctttatgaa caaaatgagt atcagtagag acagaataaa cttctacacc aagagatttc
    26761 agtgtttcgt attgctcttg aaggtcaccg agttcagttg ggcaaacaaa tgaaaagtct
    26821 gctgggtaga aacaaaaaac tgcccatttt cctttaacgt cttcatttgt aacagtgatg
    26881 aattttccat cgtgataagc ttgagctgaa aattcagcaa tttcttttcc aattagagac
    26941 atatagttgt cctccttttt tatagtttta tacttgtatt ataaaagaac aacaagatta
    27001 tgtctactca gaaacccttg attttactgg gaaatcagcg attgttttct gacaattcag
    27061 ttgtgaaata aatcacatta attaaaatga ttctaaataa gctgaagacg ccgttaaatc
    27121 aaaggttaat ttaattataa tctacagaaa atgtaattat tattaaagcg tttacaaaaa
    27181 aaactttttt gattccgtta aggcaactta atattatggt tacctaatcg ctaatattgt
    27241 aacctatttt ggcatgagat cagggaatgg ctgttatatt aattggatat gtcttgtgca
    27301 aatagtgctt gacggatttg tggaattagg gtaaaataat ctttgttgtg gcggtatagc
    27361 caagtggtaa ggcacggctc tgcaaaagct tgatcgtcgg ttcaaatccg tctaccgcct
    27421 tttatataca aaacaagcct tgattaattt cgtgaaaacg ttgttaaatc aaggttttta
    27481 ttttttgcga caggataaaa aaagagaaaa aaagaaataa agtcggtcaa aagtcggtca
    27541 aatttcccga agatgtcttt tatagcctga ttattaggta tgaaaaatac tatggccaaa
    27601 attggtaaac gattgcaaat aaaggtcaga gttcttaccc ccttaaacga tatgcgttaa
    27661 aagttagttg aactgccgtg cacctaacga tagacacggt gatgtgagag cgagtagagt
    27721 tgaatctcta cttgtttatc ttaccatagt cagctttata aagtaaccac ccattacaat
    27781 gcaactatat tcttaatgtt tttaagcatg ttattgacaa gtgttataaa atgtgttact
    27841 cttagtgagt aaattagttt ttgagttagt atttgtgttt caacatcttt atttctctct
    27901 atttttacaa atgattttta gaaaggaagt ggatgtatat ggcacaaggt acagtaaaat
    27961 ggtttaacgc tgaaaaaggt tttggtttta tttcaactga aaacggtcaa gatgtctttg
    28021 cacatttttc agccatccaa actaatggtt ttaaaacgtt agaagaagga caaaaagtgg
    28081 catttgacgt cgaagaaggt caacgtggac ctcaagcagt caatatcact aaattggcct
    28141 aatctggtta aagtaactaa agagaagcag atgttggaaa caacaactgc ttttttgatt
    28201 tctaccagtt acctttaatg attaagagtt gcgtttaagg tcttgtattt gttataataa
    28261 tagtcagttt tagtcaaaat gtattgacgt ggtgctacta agtttttaga gtagcccgca
    28321 agcaaaggag tgacagcatg ccaaccaaaa atacttcaga cagcattgaa gagtatatta
    28381 aagaattatt agctaagtca ggaatagcag aaattaagcg gtccatgcta gcagattcgt
    28441 ttcaagttgt cccaagtcag atcaactatg tcattaaaac ccgttttacg gagagtcgag
    28501 gctatgaagt agagagtaaa cgaggtggtg gaggctatat tcgtattgcc aaggttcatt
    28561 tttcagacaa acaccatctg attggcaatt taatggcaac tattgaagac tgtatcagtg
    28621 agcaagtttt cacggactcg atacaattgc tttttgacga acacttattg acagagcgtg
    28681 agggaaatat cattttggct gtggcgtctg atgatgtttt aggaactgat ggttcaacta
    28741 ttcgtgcacg aatgctctat cgcttattgc aacgaattga cagaaaggga agcaattaat
    28801 gattatgtat tcaacgaaga tgcaagacat ttttagacag gcgcagttcc aagctgctcg
    28861 ctttgatagc cattgcctgg aaacttggca tgttttgtta gctatggtag ctgtagataa
    28921 ttctttagca aatatgattt taagtgaata tgatgcccaa gtcgccatag aagaatatga
    28981 agctgcagct attttagcca tgggcaaaac ccctaaggaa cagttgtctc gtgtagactt
    29041 cagacctcaa tctaaaacct tgactaactt gttagctttt gcgcaggcta ttagccaaat
    29101 cactagggat caagaagtcg gctctgagca tgtcttattt gctattttat tgaatccaga
    29161 tatcatggcg agtcgtttgt tagaaatagc aggctatcag ataaaagata acggcaatgg
    29221 gcagccgcga ttagctgact tgcgaaaagc aatagaacgt catgcaggtt acagtaagga
    29281 aatgattaag gctattcacg aactacgtaa gcctaaaaaa acgaaaacac aagggacctt
    29341 ttcagatatg atgaagccac caagtacagc tggtgaattg agtgatttca caagagattt
    29401 gactgaaatg gcaagacaag gtttgttaga atcggtgatt ggacgtgacc aagaagtatc
    29461 tcgtatgatt caggtactaa gtcgtaaaac gaaaaacaat cctgtcttgg taggtgatgc
    29521 aggtgttggt aaaactgcgc ttgcttatgg ccttgctcaa cggattgcaa atggcgctat
    29581 tccttatgaa cttaaggaga tgcgtgtcct agaattagac atgatgagtg tggtagcagg
    29641 aacccgtttt cgtggggatt ttgaagagcg catgaatcaa atcattgatg atattgaagc
    29701 tgatggtcag attattcttt ttgttgatga actacatact attatgggtt ctggcagtgg
    29761 tattgacagt acacttgatg cggctaacat tttaaaacca gcattatcgc gcggtactct
    29821 tcatatggtt ggagcaacaa ctcaagaaga atatcaaaaa catattgaaa aagatgcagc
    29881 tctttcgcgt cgttttgcta aaatattaat tgaagaacct aatacagaag atgcttatca
    29941 gattttgatg ggcctaaaat tatcttatga gacctaccat aatgtctcga tatcaaatga
    30001 ggcagttaaa acagctgtaa aaatggcaca ccgttattta accagtaaaa atctccctga
    30061 ttcagctatc gatttattag atgaagctag tgctgctgtg caaaacatgg tgaaaaaatc
    30121 agctcctgag actttaacac caatagacca agctcttatc aatggtgata tgaaaaaagt
    30181 atctcgcctc ttagctaaag aagcaaaagg tcagatgaga aaaccaacac cagtgacaga
    30241 agatgatatt ttggcaacct tgagtaagtt atcgggaatt ccacttgaaa aactgacgca
    30301 agctgatagt aaaaaatacc tcaatttaga aaaagaactg cataagcgtg tgattggtca
    30361 ggatgctgct gttacggcta tttcaagagc cattcgtcgc aatcagtcag gtattcgaac
    30421 aggaaaacgt cctattggat catttatgtt tcttggccca acaggagtag gtaagacaga
    30481 actagcaaag gcccttgcag aagttctctt tgatgatgaa gcagcgctta ttcgttttga
    30541 tatgtctgag tacatggaaa aattcgcagc gtctaggctt aatggagcac ctcctggtta
    30601 tgtcggctat gatgaaggag gtgaactgac acagaaagtt agaaataaac cttattcagt
    30661 cttgcttttt gatgaagtgg aaaaagcaca tcctgatatt tttaacgttc tccttcaagt
    30721 attagatgat ggtatattga ctgatagtcg tgggcgtaag gtcgattttt caaatactat
    30781 tattatcatg accagcaatc ttggcgcaac agccctgcgt gatgataaaa cggtcggttt
    30841 tggggtcaaa ggcattcacc aagaccatca agctatggag aaacgtattt tagaagaatt
    30901 aagaaaaact taccgcccag aatttattaa tcgtattgat gaaaaagtgg tctttcatag
    30961 tctgacccaa gataacatgc gcgatgtggt taaaatcatg gtacagcctc tgattactac
    31021 attggcagaa aaaggtatta cccttaaaat tcagcctttg gccttgaaac atttgtccga
    31081 ggtcggctat gatgagcata tgggggcaag accattacgt cgaacgctgc aaactgagat
    31141 agaagataag ctatcagagc ttatcctttc tcgagaattg acaagtgggc atacgctaaa
    31201 aattggatta tcatatggca aattaacgtt tcacatagct taatttccat ataaaaggtg
    31261 gatcatcctt gatgtcacca aaaaaagagg ttattcttac aaaaataact tcttttttct
    31321 tgactatttt tgaccaagtg ataaaataga atataaagtt agcactcgat gaacatgagt
    31381 gctaacttta ctaaagattc ttttattgga ggaaaatttc atgttaaaac cattaggtga
    31441 ccgtgtggtc gtaagatttg atgatgaaaa agagcagaca gtcggtggtt ttgtccttgc
    31501 aggcactcat aaagagtcaa cacgaaaagc gacagtcctc gctgtcagtg aaacgggtgt
    31561 tcgcactatt acaggtgata gcgtgctacc ttcagtttca gtgggtcaag aggttttagt
    31621 tgaaaatggg catgatttag aagtaaccgt agatgatgag aaagtttcta tcatccgtga
    31681 atctgacatc attgctattg ttacaaaata aaacgacaaa tctctaaata gaaagaagga
    31741 ttgaatatgg caaaagatat taaattttca gcagatgcgc gtgctgccat ggtgcgcgga
    31801 gttgatatgt tagcagatac cgtcaaagta acgcttggtc ctaaagggcg taatgttgtt
    31861 cttgaaaaag cttttggttc tcccttaatt actaatgacg gggtaaccat tgctaaagag
    31921 atcgaattag aagatcattt tgaaaacatg ggagcaaaat tggtgtctga agtggcttct
    31981 aaaaccaatg atattgctgg tgatgggacg actactgcaa cagttttgac acaagccatt
    32041 gttcatgaag gactaaaaaa tgtgacagca ggtgctaatc caattggtat ccgtcgaggc
    32101 attgaaacag caacagcaac agctgttgaa gccttgaaag ccattgctca acctgtatct
    32161 ggcaaggaag ctattgctca ggtcgctgca gtatcatcac gctctgaaaa agttggagag
    32221 tatatctcag aagctatgga gcgtgtgggc aacgatggtg tgattaccat cgaagaatct
    32281 cgaggtatgg aaacagaact tgaagtggtc gaaggcatgc aatttgaccg tggttacctg
    32341 tctcaataca tggtcacaga caatgaaaaa atggttgcag accttgaaaa cccatttatc
    32401 ttaatcacgg ataaaaaagt gtcaaacatc caagacattt tgccactact tgaggaagtt
    32461 cttaaaacca accgtccatt actcattatt gcagatgatg tggatggtga agcccttcca
    32521 acccttgtct tgaacaagat tcgtggtact ttcaatgtgg ttgctgtcaa agcgccagga
    32581 tttggtgatc gtcgtaaagc tatgcttgaa gacattgcta tcttgacagg tggtacagtg
    32641 attacagagg atctaggact tgaattaaaa gatgctacaa tgacagccct tggacaggct
    32701 gctaagatta cagttgataa agatagcaca gtaattgttg aaggttcagg aagttcagaa
    32761 gctattgcta accgtattgc actgattaaa tcgcaattag aaacaacaac ttctgacttt
    32821 gaccgtgaaa aactacaaga acgtttggcg aaattagctg gtggtgtagc tgttatcaaa
    32881 gtaggagctc caacagagac agctttaaaa gaaatgaaac ttcgcattga ggatgctcta
    32941 aatgctacac gtgcagccgt tgaagaaggt atcgttgctg gtggtggaac agcacttatt
    33001 acggttattg aaaaagtagc agctcttgaa cttgagggcg atgatgctac tggacgtaac
    33061 attgtgcttc gtgctctaga agagcctgta cgtcaaattg ctttaaatgc tgggtacgaa
    33121 ggctccgtag ttattgacaa gttgaaaaac agccctgcag gaacaggatt taatgctgca
    33181 acaggtgagt gggttgatat gattaaaaca ggaatcattg accctgtcaa agtaacacga
    33241 tcagcgcttc aaaatgcagc ttctgtagct agtcttattt tgacaacaga agcagttgtt
    33301 gctaataagc ctgaaccagc tgcgccagcg ccagcaatgc cagcaggtat ggatccagga
    33361 atgatgggcg gcttctaagg tctctatagc attaaggatt atgaaaaaga agttgagact
    33421 aaaggtctgc ttcttactat gaaaaaagaa caaagattgg atttcgactc atgatatccc
    33481 atctttgttt ttttagttgt gatataaaat gccaatcttt gtggttacag acttttaatg
    33541 tattggaaaa tatattgttt ttctttgtca tgtgctatca tggagccaat gaaaagttat
    33601 tcccatatat gcgtggtgga ggtctgtgat gaatcatttt ggagagattt ttaaaacctt
    33661 tagagaatca aagggattac gattaaaaga tgtcgcaaag gctggtatat caacctctca
    33721 gctgtcgcgt ttcgaaaaag gggagacgga tttaaccata tcgacattta tgctgatttt
    33781 ggatgaaagt aacatgtcca ttgatgagtt tatgtatgct gtccatgatt ttcatcgtga
    33841 cgacttaaat gaactcttat caaaaagtga gggatttcgt aacaattcaa gatagtgatg
    33901 gcttgaagca gttacttcat gctcaactag catcaactga taagaaggaa atgttcacgg
    33961 atatcttttg gcatatctag catgtctaag tctgctatgg catgccattt tgggagtccc
    34021 tcacgcgact ctgtcagtag ctctccttca aaggattcac atagaaagtc gtaatagaca
    34081 tagcactctt tttttgtcag gcttgataaa gctagaaata ccctttaatt gaaggtttaa
    34141 agctgtcaga cctatttctt cttttagttc tcataaagcc gcttcaaaga aagattcaca
    34201 gggctcgact ttaccttctg gctggaccca tcctggtaag ttatcgtgtt gtctatggag
    34261 tcataaaatc atatcgttat gtttgacaca catattgccc cagtctctaa ccgttgctat
    34321 caccagattc accacctttt agtatagtat aagaaaaaag gggtgaaaga ggtttcttgt
    34381 tgtttttttg gcttaagatt tctttaagtc ttatctagta tactaatgtc attccttaat
    34441 aggataagta tttggttgta gaagtatcgt cttaaactaa cgctatctca accgattccc
    34501 aacttattca cccctcctat accgataagg tctcacaaag tgtgagacct tattttttga
    34561 cttcagctct agttatggag aaacttgttg atacaagctt ttttgggtta tgggttatgg
    34621 tattatgatc tattcaagcc tttcttgttt ttgggttttt cttcaaccaa cttggcttca
    34681 atagctttta agcgtttgaa ggttttttca gcgcgatcta gtgaaatctt atcagctttt
    34741 tcttgagcag ctttggggtc actgacgact aagtcactga tttctttggt gcttttgtat
    34801 tgttcggcta tccaggtttt ttccaagcct ttcatctcat caatcacttt agttccaaat
    34861 ggttttggat gagcagccac gagatcatta atgtgtgaca ccgtccaata ccatgactta
    34921 tcgttatatt gggtgctttt ttcttgatag gcttcatagg ttctactgat attgccaagg
    34981 tatggcaagt aaggggcatt acgaggactg ccaatagata gccacatgac accaccaccc
    35041 aattcagcag ggatgtcttt tttcagttgg aaaatatgag cttccatgac attaggattt
    35101 gagatgggat aagcataacc tttaacagct tttttggatt ttggtttacc cttaccgtct
    35161 aatgccatct gatcaagtgg tttaagatcc agcccctcaa aacgattacg ttgcagtttc
    35221 atcgcatctt ctagactgaa ggtcttatca gtgctttgga gcaactcata attgctgtct
    35281 ttataagtta cttttgaatc agggtcaagt gatttaatcc ctgagaaaga gcgagagcgg
    35341 ttggcatcgt ttagtggtgg attgtaagat ttggcaatat gaaatttacc atcaacttcg
    35401 gtataagatt tggctttttt agcgactttt tccacatctt ctgaggcaat cgtattttcc
    35461 ttatcgttga aatcgacatg tccgagatag aaggtatttg gaaaaacagc gtatttatca
    35521 tctgggaatt tgatggcaac atattggtga ccagagagaa tttccatgta ccagatgcca
    35581 tctttgtcag ccaaggtaac aatatttcct tcagcagctc ctttttcagt gactatttta
    35641 gcaatcaatg cgacaccttc acgggctgtt ttgacgcttg gtaaaatgac actggccata
    35701 gatgattccg ctaagccatt tttgacgtaa ggatcaattt tttggatggc atcattagct
    35761 gaagctgata ctgttgcaga catagagacg ccaaactcat taaaaccagc ttcgtcataa
    35821 acgcctttgt taggtgttac atcaggaatc gcactatagc gataagaatg ctcaggcaac
    35881 gggtactcaa aaccattaga gaggtctttc catttttcgc cagcaggatt gtcctttgct
    35941 agacgtacaa tgaaattttt attatggtgt ggctcaagat cttctgttcg accataaaga
    36001 agagaaccgt ctttggttaa atctttgcca ataataaaac cagtacaagc ataactaaca
    36061 ctttgaagcg aaaaggctgt cagaatactt aaaactccaa gtgatatttt tttgtttatc
    36121 atgcgaatcc tcctagtaaa cgataacgct ttcacagaca ttataaaatg ctctttttaa
    36181 aaattcaagt cttttatgaa gtgttttgaa attgttagct atctaaatat tatgagaatt
    36241 ctctcttgaa ttttggctta acggccccta aaaacgtata ggcagtgaac tatctaggtt
    36301 tcttcgaaaa tccattagcc atttatgata taatagtaaa gaaaaacata gaaagttggg
    36361 gtctgttgat gaccaaacca tttcatcata agaaactaaa acaaataact ataatagctg
    36421 caactagcct ttttttattc ctgatcagtg gtgcctttta ctactctaaa aatcattgta
    36481 ttaatgccta tcttaaagct cgatcagccc aatcaggacc agtttttgag aatatcaaag
    36541 catatctagt ctgggatgat actaatgagc agatcacaaa tgacgaggcg atgtatacta
    36601 agtttagaag gtatagtcaa aaagaattga ggcaaaaaaa gcaggattta aaagccgcta
    36661 gtcaagatag tgccgttcaa gtaaagtctg ttggtcgtcg tttttggatt tttcctgatt
    36721 atcggattgc cataaaaccc atggatttaa cgataaaaac gaatgtgcct caagcagacg
    36781 ttcttttaaa tcaaaaaaaa gttgctgttt ctgattcaga acagttctca gtcaagcttg
    36841 atcggctacc aacggcagaa tataccgcaa gtatcagagg caaacacaac gggcgaaaca
    36901 ttaaagtcaa caaatcatat gatggtgata atcccgtgct agatttgagt gtgtctttta
    36961 gaactttttt ggtaacaagc aatgctaagc aaggagatct ttacttcgat gataaccata
    37021 ttggcacatt aaaagatggt caattacaag tagaagatta ccctgttaca gaaaacgcac
    37081 aagcttatat gaaaacaaca ttcccagatg gtgagttaag atcacaaaaa tatgctctag
    37141 ctgatgttga agaaggagca accctggaga ttttagttac agatctttta gaagaggaca
    37201 aggcagggga gctattagta tcagcttttg atcagctgat gcactacctc agtacaggtc
    37261 aagactcatc taacttacgt agcgtctttg aggcagggtc tagcaacgca ttttatagag
    37321 gattgaaaga gtccataaaa gcgaaatttc agacagatac aagaaaagcc agccgtctca
    37381 atattccatc tatccttttg acaacaatga ctcaagtggg taaaacaact tacgtgcttg
    37441 atttcacagc tacctacgaa tttttgtatg acaactcaac agatcctgag cagcatacct
    37501 ctggacatat taatcaagac ttgactggaa aagtgacggt aaaaaaagtt ggacagcatt
    37561 accttatcag ccagtccggc tccaaaaata ttactgttgt taaagaggac aatcaactca
    37621 aagcgccatc tgtctttcct gagtctattt tgggaacgtg gacaggccaa gccaatggtt
    37681 tgagcatcca tatgtctcta gcatcagatg gaacaattac gactaaagtt gaagatcaaa
    37741 aaggcaaccg ttctaaagaa actcggacag ctaaaattag taaagttgaa gacaaaggca
    37801 atggttttta tctttatacg ccagatcctg gaagtgacat aagcgcctta gttccagaag
    37861 gaggattggg gggtgcaaat gtcaaatatg cttatggttt caaaatatct ggtaaaacag
    37921 cctctccagt ggtgtggcag gcagcattaa cacatgaatt tgattatacc aagccacttt
    37981 cgggagtaac tttgcaaaag caaccataaa ggatgcaaag ccacaaagaa cagtagaaac
    38041 aatgttatca ctcaaataca gtgctatgaa aacagctaaa ctcaaaaggc taggagctta
    38101 tttcctagcc ttttgagcgt ttaagactgc gtcaaatgta gctgatttag cttgcacgca
    38161 aatgtcttct tgggttagag ggtgtttcaa acgtaattga taagcatgga gcatcagtcg
    38221 agcacaatct ttttttccat tagaatacaa aggatcacca aataaaacat gaccttgatg
    38281 ggctaaatgg actcgaattt gatgagtccg tccagtttgt aattgacaat tgagtaagct
    38341 tgccgttttg tggaagtttt tgactagagt tacctcagta atagctttct tgccatttat
    38401 cggatcgaca acacgttttc tgcggtcatg tcgatgacga ccaattggat gatggtaagt
    38461 taccctagga ctgtctaagg atccgtgaac tagtgccaaa tattctcgat ggatgtctcg
    38521 tttttctaaa agacgattaa ggattggtaa gataaaaggt gttttagcaa ataaaatggc
    38581 cccacttgtt tccttatcca gacgatgaac cacatagcag gtctgtcccg tgtaagcaga
    38641 cacgtggttg agcagcgcca actctgttgg atcattgccg tgtgttttca tcccttcagg
    38701 tttgttaaca ataatgatgt gttcatcttc ataaaggcag gttacttttt cggcctgccc
    38761 catcacgata atcttttcag gataatcttc gtgatcgaaa aaaagtttga cctgatcacc
    38821 gtatttaaca caactttgcc agttaacagg atgaccatta ataagaacat gttttttagt
    38881 tctcagaaaa tgcctaattt tacggggaat taacaattgt tcctctaata atgctttgac
    38941 cgtcaattct tcaaagggat ttttcagtgt gacagtaaac ccctttgatg gtttatttgt
    39001 tgtcttcatt gagccagtta tggtattttt caacaaggtt gatagccatt tgggtggcaa
    39061 gagcagcaga gaaagcttca gtaccttcac gctcgtcatt agctacctcc aagcgagtct
    39121 catcgtaaat ggcttttaga ctttctgcgg ataaggtttc tttaaaagct tggaatttat
    39181 tcattggtta aacccgtcct tctatgttta atgtgagctc ctgattaatg caaggaattc
    39241 aatgctatta tagcatgttt ctaatcaaaa cgtgccactc aaaaaggtaa aaacgttatt
    39301 ttgctaatcc atataataag aaagtgtctt aaataagacc tcttgataga gtttgtaaca
    39361 gaaatgatat gaaactaggt gctaacgcag aaatagaaaa caactaacgt gttgcgaagt
    39421 tggaacagtc ataaaaaata gcaagataaa acttatcaac acacagttac ctaagacgat
    39481 gagtcaaaca ggtatgttct ttcaaaacaa ccccatgagg ttgaacgacg tatcggaaaa
    39541 atacggatta gtttggcctt atgataggtc cttctctatc gtaaaagaag tcaaccaaag
    39601 gtttttaata taaaatatta agaaaaattg ttataattat gataaaaaca taatcgtaaa
    39661 atagaggaaa aacaatgaaa acaattagac gttatgatgt taacgaagat agagggcata
    39721 caggccttgt agaagcagga gatttctatt atttaaacta ttgtgtcggc aatgtcggac
    39781 aagacattga aagtcagatt aatggagcct ttgacgagat ggagcgccga ttagcattgg
    39841 tggggctcac attagatgca gttgttcaaa tggattgtct atttcgagac gtttggaaca
    39901 tacctgtgat ggaaaaaatg attaaagagc gctttaatgg cagatatcct gctcgaaaat
    39961 cgattcaaac ggaatttgca catcacggtg gaccacaagg actactcttt caagtggatg
    40021 gtgtggctta ttcaaaacat atttcgatga cctaatcaag gttactttaa gatcatagtg
    40081 gtataataac gttatgagat ttctagaact tttacaaaag aaattttttc ctaaagcata
    40141 tcaggaaaaa caattcttaa tgcatcaaaa agcgcgttta acgccacaac acaatcaaaa
    40201 gcagtattcg ccaaatgcca atcatttgga ctcatcagct accaaaaact cagaacaaga
    40261 ccctgcaata gctctgcaac gcagtagagc ctatgaagga agccctaaaa gtcggcccgc
    40321 ttggttgcaa aagttggaag ctgttttgcc gtctcctcaa catccaattc ggcgtttttg
    40381 gcgccgctat cacatcggaa aactgctaat gattctgatt ggaactcttg tcttactctt
    40441 aggatcatac ttgttttact tatcaaaaac agctaaagta tctgatttac aagatgcctt
    40501 gaaggctaca actgttattt atgatcacaa aggagagtat gcaggcagtt tatctggtca
    40561 aaaagggagt tatgttgagc tcaacgctat ttcagatgat cttgagaatg ctgttattgc
    40621 cactgaggat aggacttttt acagtaatag cggtattaat cttaaacgct tcttattggc
    40681 ggtagttacg gcgggccgct ttggaggtgg ctcaacgatt acacagcaac tggctaaaaa
    40741 tgcttatctc tcacaagatc agacaattaa acgaaaggcc cgagagtttt ttttggcgtt
    40801 agagttgacc aaaaaataca gtaaaaaaga tattcttact atgtacctta acaactccta
    40861 ctttggaaat ggagtttggg gagttgaaga tgccagtcaa aaatattttg gaaccacagc
    40921 tgctaactta acactggatg aagctgccac attagcaggt atgctcaaag gacctgaaat
    40981 atataaccct taccattctc taaaaaatgc tactcaccgt agagatactg ttttaggagc
    41041 gatggttgat gccaaaaaga ttacccaaac aaaagctcag caagctagag cagtagggct
    41101 aaaaaatcgc ttagctgata cttatgttgg taagacagat gactacaaat acccatccta
    41161 ctttgatgct gttattagtg aagcaatagc aacttatggt ctttcagaaa aagacattgt
    41221 taataatgga tacaaagttt acaccgagct agatcaaaat taccaaactg gcatgcagac
    41281 gacttttaac aacgatgaac tgtttcctgt ttcagcttat gacggtagct ctgctcaagc
    41341 agctagtgtt gctttagatc ctaaaacagg aggtgttaga ggtctgattg gtcgtgtgaa
    41401 tagtagtgaa aatccgactt tcagaagttt taactatgcg actcaagcaa aacgtagtcc
    41461 cgcatcaaca atcaaaccac tcgtggttta cgcgccagcc gttgcttcag gatggtcaat
    41521 tgaaaaagaa ctaccaaata ccgttcaaga ttttgatggc tatcagccac ataattatgg
    41581 aaattatgaa tcagaagatg ttcctatgta tcaagcatta gcaaactctt ataatattcc
    41641 agcagtttct acattgaacg atatcggaat cgataaagcc tttacctatg gtaaaacatt
    41701 tgggttagat atgagctctg ccaaaaaaga gttgggggta gctttaggtg gcagcgtgac
    41761 aaccaatcca ttggagatgg ctcaggcata tgctgccttt gccaataatg gagtaatcca
    41821 tcctgcgcac ttgattaacc ggattgaaaa tgccaggggt gaagtgctca aaacctttac
    41881 tgataaggct aaacgtgttg tcagccagtc tgttgcagat aagatgacag ccatgatgct
    41941 aggtaccttt tcaaatggaa cagcagtcaa tgctaacgta tatggctata cactagctgg
    42001 taaaacaggg acgacagaaa ccaacttcaa tcccgactta gcaggcgatc agtgggttat
    42061 tggttatacg ccagatgttg ttattagtca atgggtagga tttaatcaga ccgatgaaaa
    42121 tcattatcta acggattcaa gtgcaggcac ggcttcagct atttttagca ctcaggcatc
    42181 ttacattttg ccttatacca agggcagcca atttcatgta gataatgcct acgctcaaaa
    42241 tggtatttca gctgtttatg gagtcaatga aacaggtaat caatcaggag ttgacactca
    42301 atctattatt gatggtttaa gaaaatcagc acaagaagct tcgcaatcac tatcaaaagc
    42361 agtcgatcag tcagggttac gtgataaagc ccaatctatt tggaaagaga ttgttgacta
    42421 ttttagatag cttgcagtat tttagagatc gtgttaaaat aatagttacg gaggcgttat
    42481 ggcacagaaa aaagcgagtc tagcgtgtgt cgaatgtggc agtcgtaact actctatcgg
    42541 agtgagtagc acgcctaaac caacacgtct agaagtaaat aaattttgca aatactgcaa
    42601 aacatatacc ttacacaaag aaacacgtta aaaaggagag cctgatggga ttcattagcg
    42661 ggacttttaa agtcttaaaa gacacaacat ggccaaatcg taaacaacgt tggaaagatt
    42721 ttatctcagt tcttgagtac acagctttct ttacggtgat tatctacatc tttgaccagc
    42781 tcttagcaaa gagtgtatta gcactgatta atttatttta ggtcaagatt ggtagaaatg
    42841 ataagcttgc ttggaaaagc ctaggctggg agtattccca gccttttttg tagtatcaat
    42901 aatctaatta gtaagctttt taaagcccat actggtttga gctattcaaa agcataaagg
    42961 taaaggcaat gtagcaacga tctccaaacc aagttcacta aattagcttg taacagagtc
    43021 aacgcataaa cagcctggcc tcacttgctt agcacattaa aaatagcaat gaatattatg
    43081 gttgaggaaa acagcctgat agtctgctaa atcttcggga tgataagcct tgacctcagc
    43141 catctcatac tccctaccta gaagtttgta cttgttttct ccaaattgat ggaaaggtaa
    43201 cagttgcact tggtcaatct caagctgatt aaagagctct gagaaggctt tagcatcgtc
    43261 tagtgaatca ttaaattggg gaataacagg gatgcgcaag acgatttctt ttccagcttg
    43321 aaaggcataa tgaatatttt tgataatcaa gtcattacga accccagtca ctttttggtg
    43381 tctgagctga ttataatgtt tcaaatcggt atagataaag tctacgtagt caataagggt
    43441 gacaaattgc tcgtgtttgg caaaggcggt ggtttcaatt gcggtgtgaa gacctgctgc
    43501 tttggctgct tttaacaggg ctaaggcaaa gtcaaattgg gcaaagattt ccccccctga
    43561 cagggtcatg cccccacctg actcttcgta gaaatcaagg tctttaagga cttcttcgat
    43621 cacctcgtcc acggtttttt cctctccaac tattttagta ttaagcccat cacttgttaa
    43681 catctgctca ggtgcttttt gttgggattc gggattggca caccaaggac atctcaaggg
    43741 acaacctttt aaaaaaacgg ttgtccgaat accagggccg tcatgaatac taaaatgttg
    43801 gatgttgaaa acaataccgc gatcagtcat aagtgtgaat tcctttcgtt gtaaaaccat
    43861 cataccagat aaacgtaaat aaatcaatta cgaatgaaag taaatatccc ttttctttta
    43921 agaaaataac tggtacaatg aaagggaggt gaatgatgaa tcgattagaa cgtattattc
    43981 aactggtttc ccagaagaaa aaaatagatg ttaacagttt atcagagcaa ttagaagttt
    44041 cgaaagtaac cattcgaaaa gaccttgata agcttgaaag taagggcttg ttgcgacggg
    44101 aacatggcta tgcggtgcta aacagtggcg atgacctcaa tgtacgcttg tcctacaatt
    44161 acaacataaa gcgacggatt gcagaaaaag cagctgaatt ggtgcaagac aacgatacca
    44221 ttatgattga atctggctca acctgtgccc ttttggcaga agtgctttgc cagaccaaac
    44281 gaaatattaa agtcattacc aattcgtgct tcattgccaa ctacattcgt caatatagta
    44341 gctgtcaaat cattttactg ggcggttatt accagcccaa ttcagaggtg actgttggtc
    44401 ctttattgaa agaaatgata tccctttttc atgtcaatcg tgtctttgtt ggaacagacg
    44461 gtttcaacaa agatcttggc tttatgggca aggacatgat gcgctcagaa ggggtgcgtt
    44521 acatggcaga tgctgctgag gaagtggtga ttctgaccga ttccagcaag ttttcaaaaa
    44581 ccagtctggt acaccagttg tcattagctg atgtcaatcg cgtcattaca gaccaagcac
    44641 tagacaagca aacacaagag ttattaagtg ctagtggtct tgttcttgat tttgtttctt
    44701 agaaaatagg tggctagatg aaagaagaac gcagacggct ccttgccaag gtggcttact
    44761 tgcattatgt tcaaggcaag agccaaacct tgatttcaaa ggaaatgaat atctaccgaa
    44821 caacggtttg tcggatgtta gcaaaagcca aggaagaagg cattgttcgg attgaaattg
    44881 ccgactatga tgctgattta tttgctttag aagaatacgt gcgaaagcag tatggcttgg
    44941 aaaaactaga ccttgtgccc aaccaggttg aagatacccc aatggatacc ttgacaaatg
    45001 ttgctaaaac agcagctgag gtttttagac atgttgttaa agatggcgat aagatcggtc
    45061 tgtcctgggg agcgactctt tcttgcttga tggatgagtt aaatccaaaa gcgatgaaag
    45121 atgtgtttat ttatcctttg gcaggaggac ctagccatat taatgccaaa taccatgtca
    45181 ataccctggt ttaccgcttg gctcgcatct ttcatggtaa tagcgctttt atgaacgcta
    45241 tggtcattca agaagacaaa cacttggcta agggaatctt acaatctaaa tactttaacg
    45301 acattttaac aagctgggac cagctagatc ttgccttagt aggaattggt ggggagccta
    45361 atagccttga gcagagtcaa tggcgtgatt tattaaccag ctcagaccat gaccaactca
    45421 aatatgaaaa agccgttggt gaagtttgtt gccgtttctt tgatcaggca ggtcagccag
    45481 tttacactgg cctacaggat cgaactattg gtatttcgct agaacagttg aggcgtgtcc
    45541 ccaaaacaat ggctgttgca acaggtaaac acaaggctaa ggcaatattg gcagccttaa
    45601 aagcaggttt tatcaattat ctagtgacgg ataaagaaac catgctagct gttttagcgt
    45661 tagatgaaga tattgatctt aataacgttc ttctttaata gaaattcaaa gatactatga
    45721 tatttataga ggcccaagac caaacggtca gggctttttt tgtttgatgt gctgataaaa
    45781 cagagattgc tcaaatgtgc agttaatgta ttggcacaaa agtgcattaa ccaaaatata
    45841 gcacaaaata tattgaccct aaaaacaaaa atgatacaat aaacttacca acgaaatgta
    45901 aacgattaca aacataataa catcaccttc gaaaaaaaga ctcattgccg ttacatcaca
    45961 tgaaaaagaa aaaaaggaga caccaatgca agttattgta ccagaccaaa tcattatggg
    46021 cttaatttta aatgcaggcg atgccaaaca acatatctac caagctctga aatgtgctaa
    46081 agaggacgat tatgccacta gtgaaaaaga aatggcctta gcagatgatg ccttattaga
    46141 agcccacaat ttacagaccc agtttcttgc ccaagaagct agtggtaata agtcagagat
    46201 tacagctctt tttgttcact cacaggatca cttgatgacg accattacag aaattaatct
    46261 catcaaagag attattgacc ttcgtaaaga attggcaact aaataagttt tcatgactaa
    46321 aaaggagatt gacatgatta aaattggatt gttttgtgca gcaggatttt caaccggtat
    46381 gttggtgaac aacatgaaag tagctgctga aaaaaaaggc attgattgtc agattgaagc
    46441 ctatgctcaa gggaaattag cggactatgc accattactt gatgtggcac ttttaggccc
    46501 acaggttgcc tatactctgg ataaatcaga agctatttgt aaagacaatg atatccctat
    46561 tgcagttatc caaatggctg attatggaat gttagacggc aacaaggtgc ttgatttagc
    46621 ccttagcctt gttaaagaat aggagtcaat catggcaaaa atgaatatgc aaaacatcat
    46681 caagccaatc atgacatttg tgaatatgcg cggcatcatt gccttaaaag acggtatgtt
    46741 agccatttta cctttgaccg ttgtggggag tcttttcctg attgctgggc agattccttt
    46801 ccaaggggtt aatgatgcca ttgctagtgt gtttggtgct gactggacag aaccctttat
    46861 gcaggtttac catggaacat ttgctattat ggggttgatt tcctgttttg caattggcta
    46921 ttcttatgct aaaaactcag atgttgaacc tctcccttca ggggttctat ccttgtcagc
    46981 cttctttatt ttattgagat catcttatgt tccagcagaa ggtgaagcta ttggagatgc
    47041 tatcagtaag gtttggtttg gtggtcaagg gcttattggt gctattgtga ttggtttaac
    47101 ggtcggagct gtttacacaa catttatccg tcgtcatatc gtcattaaaa tgccagatca
    47161 agtgccacaa gccatcgcca aacagtttga agccatgatt ccagcctttg tgatctttac
    47221 cttgtcaatg cttgtgtata ttattgccaa gtcagtaaca ggtggtggca cctttattga
    47281 aatgatttat gatgtcattc aggtaccgct gcaagggtta acgggttctc tttatggcgc
    47341 actaggtatt gccttcttta tttcctttct ttggtggttt ggcgtgcatg ggcaatctgt
    47401 ggtcaatgga attgtcactg ctcttctctt atcaaatcta gatgccaata aggccttgat
    47461 ggcagcaggt gagttatccc tagacaaggg tgcccatatt gtaacccaac aatttttaga
    47521 ctctttcttg attctatcag gttctggcat tacctttggt ttagtggtag ctatgatctt
    47581 tgcggctaaa tctaaacaat ataaggcctt aggtaaggtt gcagccttcc cagccctctt
    47641 taatgttaat gaaccagttg tctttggttt tccaattgta atgaatccag tcatgttctt
    47701 gccatttatc ttggttcctg tcttggcagc tctcacagtt tatggcgcca ttgctattgg
    47761 tttcatgcag ccctttgcag gagtgaccct tccgtggtca acaccagcca ttatctcagg
    47821 gttcatggtt ggtggctggc aaggagctat tgtgcaaatt cttatactca tcatgtcaac
    47881 gttggtgtac ttcccattct ttaaaatcca agataatatg gcttatcaaa atgaacaagc
    47941 tactgaagag tcatgattta gcgtccacac aaattcttga gaccatctcc agttagaggt
    48001 ggttttttag aaaaagaaga gcgatttatc aggcctatat tgcacaatag tgcaaaataa
    48061 agtgactatc tcttcttatg gttaaggata cgcaaaagtc gcaatatatg ataaaatagt
    48121 tacgaaagaa acatttataa tttcgtaaga aagaaggaga ccccgcatga ctgaaacaaa
    48181 aagcccttat tttggacatt taacagacag aatggctcac tatcgtgaag ctgtgttaga
    48241 caagaaacct tatattgatg ctgagcgtgc tattttagcc actgaagcct atcaaaaaca
    48301 ccaaaataag ccagcaaacc tcaaacgcgc ttacatgctt caaactattt tggaaaacat
    48361 gaccatctat attgaggatg aaagcctcat tgctggtaac caagcctctt caaacaagga
    48421 tgcaccgatt ttcccagaat acactctgga atttgtcctc aacgaacttg atctttttga
    48481 aaaacgagat ggggatgttt tctacattac cgaagaaacc aagcaacagc tccgagacat
    48541 tgccccattc tgggaaaaca ataacttgcg tgctcgctgc ggtgtcttgc taccagaaga
    48601 agtgcaagtg tacatggaaa caggtttctt tggcatggaa ggcaagatga actctgggga
    48661 tgcccattta gcggttaatt accaaaaact cttggaacac ggcttgaagg gatttgaaga
    48721 acgggcacga gctgccaaag ctgcgcttga tctgaccatt cctgaaaaca ttgacaaata
    48781 ccatttttac gactctgttt ttattgtcat tgatgcagtc aaaacttatg ccaaacgcta
    48841 tgccaagttg gctagagaat tagcaaaaac ggctaaacca gaacgtcaag cagaattgtt
    48901 agacatcgcg cgcatttgtg acaaagtgcc ttacgaacca gcgaaaacct ttgcagaagc
    48961 tgtgcaatct gtctggttca ttcagtgtat tttgcaaatt gaatcaaacg ggcattccct
    49021 atcttatggc cgctttgacc agtacatgta tccttatgtc aaagctgatt tggaggctgg
    49081 ccgtgaaaca gaagacacaa tcgttgagcg tttgaccaac ctctggatta agaccttgac
    49141 cattaataaa gtacggagcc aagcccacac cttctcatca gcaggtagtc cactttacca
    49201 aaacgtgacg atcggcggtc aaacacgcga taaaaaagac gcggtcaacc cattgtctta
    49261 ccttgtccta cggagtgtgg ctcaaaccaa attgccacaa cctaacttga ctgttcgtta
    49321 ccataaaggc ctagacaata cctttatgaa cgaatgtatt gaggtcatga aacttggttt
    49381 tggtatgcca gccatgaaca acgatgaaat catcattcca tctttcatca aaaaaggtgt
    49441 ctcagaagaa gatgcttatg attactcagc cattggctgt gtggaaacag cagttcctgg
    49501 aaaatggggc taccgttgca cggggatgag ttacatcaac ttccctaaaa ttttgctgat
    49561 taccatgaat gacgggattg acccagcatc aggcaagcgc tttgcaaaag gacacggtca
    49621 cttcaaagaa atgacctctt atgaagaatt aaaagcagca tgggatgcca ccctgcgcga
    49681 aattacccgc atgagcgtca ttgttgaaaa tgctattgac ctcggtcttg aacgtgaagt
    49741 accagatatt ctttgctcag ccttgacaga tgattgtatc ggtcgtggca agaccttaaa
    49801 agaaggcgga gctgtttacg actatatctc aggccttcaa gtcggtattg ccaacctatc
    49861 agactcccta gcagcgctga aaaaattagt gtttgaagaa ggtcaattaa ctccagaaga
    49921 actctggcac gcccttgaaa gtgactttgc tggagaacgc ggagaagaca tccgccaaat
    49981 gctcatcaat gacgcaccaa aatatggtaa tgatgatgac tacgcagata gtttagtcgt
    50041 cgaagcttac gacacctaca ttgatgaaat tgccaaatac ccaaataccc ggtatggccg
    50101 tggcccaatc ggaggtatcc gctattctgg gacatcatca atctcagcca atgtcggaca
    50161 aggaaaaggg accttggcga caccagacgg tcgccatgca gggacacccc tagcagaagg
    50221 ctgttcacca gaacacagca tggacaaaaa aggccctact tcagtattga aatctgttgc
    50281 taaattacca acagatgaga ttgtcggagg agtactactc aaccaaaaag ttaacccaca
    50341 aaccctggcc aaagaagaag acaaactgaa attaatggcc ttacttcgta ccttctttaa
    50401 tcgcctacac ggttatcata tccagtacaa tgtcgtgtca cgtgaaacct tgattgatgc
    50461 ccaaaaacat cctgaaaagc accgcgacct cattgtccgt gttgccggct actcagcttt
    50521 cttcaatgtg ctctcaaaag caacccaaga tgacattatt gaacgtacag aacacacact
    50581 ttaggagaaa ataagtatgg aatacatgtt agacacttta gatttagaag cgattaaaaa
    50641 atggcatcac attttgccgc tcgcaggtgt gacctcaaac ccttctattg ccaaaaaaga
    50701 aggtgacatt gatttctttg aacggattcg agaagtccgt gctattatcg gtgacaaggc
    50761 ttctattcat gttcaagtga tcgcccaaga ttacaaaggc attttaaaag atgctgctga
    50821 aatccgcagg caatgtggag atagtgtgta tgtcaaagta cctgtaacca cagagggcct
    50881 tgcagccatt aaaaccttga aagccgaagg ctatcatatc acagcaaccg ctatttatac
    50941 gaccttccaa ggactattag ccattgaagc tggcgctgat tatttggctc cttactataa
    51001 ccgtatggaa aatcttaata ttgatccaga agctgtcata ggacaattag cagaagctat
    51061 taaccgcgag aatgctgata gtaagatttt ggcagcaagc tttaaaaatg tggctcaggt
    51121 caataaatcc tttgctctgg gagcacaagc catcactgct ggcccagatg tgtttgaagc
    51181 aggatttgcc atgccatcta ttcaaaaagc ggttgacgat tttggcaagg attgggaagc
    51241 cattcatcac cgcaagagca tttaagaaat agcatcagta gtaatcacat cattaggaat
    51301 cacagataaa ggagttcaac gatgaaagta tttgcaagtc catctcgtta cattcaaggt
    51361 aaaaatgcct tgtttaccaa tgctaaaacc ctaaaacaac taggggatag tcccatttta
    51421 ctgtgtgatg acgtggtgta tggcatcgtc ggagaaagat ttgaaagcta tttgatagat
    51481 aatagcatga ctcctgttca cgtagccttt aatggcgaag cctcagataa tgaaatcagt
    51541 cgcgtggttg ccattgccaa ggaaaatggc aatgatgtta ttattggtct tggtggtgga
    51601 aaaaccattg acagtgctaa ggctatcgct gatttacttg ctgttccggt gattattgcc
    51661 ccaaccattg cttcaacaga tgccccaacc tcagccttat cggttattta caccgatgaa
    51721 ggagcctttg aaaaatacat tttctattca aaaaatccag atcttgtttt ggttgacaca
    51781 caagtgatct gtcaggcacc aaaacgctta ttggcgtcag ggattgcaga tgggttagca
    51841 acatgggtag aggcgcgtgc tgttatgcaa aaaaatggag acaccatggc aggtggcaat
    51901 caaaccttgg caggagttgc cattgccaaa gcctgtgagc agaccttgtt tgcagatggc
    51961 ctaaaagcga tggctagctg tgacagacaa gtcgtgaccc cggccttgga aaatgtcatt
    52021 gaagccaata cacttcttag tgggcttggt tttgaaagtg cgggtctggc tgctgcacat
    52081 gccattcaca atggctttac agcgctgaca ggtgctattc atcaccttac ccatggtgaa
    52141 aaagtcgcct atggcacgtt gacccaactc ttcttggaaa atcgttcgcg tgaagaaatt
    52201 gaccgctaca tcgactttta ccaagctatt ggcatgccaa caaccctcaa agaaatgcat
    52261 ttagacacgg ctactcaaga ggatttctta aaaattggcc gtcaagcaac catggcaggc
    52321 gaaaccatcc atcaaatgcc atttgttatc agtccagaag atgtagcagc ggctttggtt
    52381 gctgttgatg cctatgttac gagtcgatag gctaaccaaa aagagatttc ttatttgcca
    52441 gataacctca aaaaaacaag tctagggagt cacttcctag acttgttttg cttattgttg
    52501 gttggtaata gcacttttag cggggtcatt gtgggcaata cggctggcag ctgctgccgc
    52561 aatggcgcta acaatatcat ctaaaaaggt atggcaggag tagccatctt tgtggttgag
    52621 tttgtcaaca attcctggtt ttgtcttgtc cagataccca taattggtaa aaccgataga
    52681 cccatagagg ttaacaatgg ataaggccag gatttcatca atcccataca gcccttggtc
    52741 ggttttcaag atactaagga gtggctcaga cagctggttg gcttcagcca atttatccaa
    52801 ttctacccca gtgataatag cattttgcac ttctcgtttt gcaagcaccg cctcaacgct
    52861 ctctaaacat tctgccatgg tcaaattggg aatataatca ttctgcaaaa aaagcaccag
    52921 ctctgcaata gcttctaaag aaccccctcg ctcagccagt aattgataag acacttctcg
    52981 taggtgatgg tcagttgaca tgtgagacct cctttttttc tttattgtaa cataaaagat
    53041 ttcgagaaac ctctacaaca gctaaagagg ggatagttct tttttaagag atgttgacta
    53101 ctgagatccc ctacttggta aaagtcaaag aaacgatagt aaagtaacca tcttacctag
    53161 gcctaaatac aatcaccacc cctaggtgac acacagcact atttcaaaaa tgatattagt
    53221 atggcaaaac aaaaagacaa cgccttcttt tttctcctta ctatctcctt taattttcat
    53281 attttttaaa aaaactattg ataaactagt taagtaagcg tatactatgg ttagtgagcg
    53341 aaattagaaa agaggacaag catatgaatc tacttggatc aagacgggtt ttttctaaaa
    53401 aatgtcggct agtaaaattt tcaatggtag ctcttgtatc agccacaatg gctgtaacaa
    53461 cagtcacact tgaaaatact gcactggcac gacaaacaca ggtctcaaat gatgttgttc
    53521 taaatgatgg cgcaagcaag tacctaaacg aagcattagc ttggacattc aatgacagtc
    53581 ccaactatta caaaacctta ggtactagtc agatcactcc agcactcttt cctaaagcag
    53641 gagatattct ctatagcaaa ttagatgagt taggaaggac gcgtactgct agaggtacat
    53701 tgacttatgc caatgttgaa ggtagctacg gtgttagaca atctttcggt aaaaatcaaa
    53761 accccgcagg ctggactgga aaccctaatc atgtcaaata taaaattgaa tggttaaatg
    53821 gtctatctta tgtcggagat ttctggaata gaagtcatct cattgcagat agtctcggtg
    53881 gagatgcact cagagtcaat gccgttacag gaacacgtac ccaaaatgta ggaggtcgtg
    53941 accaaaaagg cggcatgcgc tataccgaac aaagagctca agaatggtta gaagcaaatc
    54001 gtgatggcta tctttattat gaagccgctc caatctacaa cgcagacgag ttgattccaa
    54061 gagctgtcgt ggtatcaatg caatcttctg ataataccat caacgagaaa gtattagttt
    54121 acaacacagc taatggctac accattaact accataacgg tacacctact cagaaataat
    54181 accaaaaggc tagacctctg ctcactaggc ctagcttttt acatcaaaaa aagcaatgac
    54241 tatagaaagt aaaaatacta gaaaaagcaa tgattgccgt cattgctttt tatgatttgt
    54301 caaaaagtaa aaagcaaatt gaaaaaatcg ccctggagct gttgagataa actacaccat
    54361 ttattaagat accaaatcaa aaagctaaca ccataagagc gttagctttt taaagagcct
    54421 caggacagtt tatgtttaat ggcttctagg taggtttgaa acatatgatg gatcgttttg
    54481 caattaagta attggaagat ctccaagatc tgcacacact tttgctcagc cccctcttta
    54541 ccatttatga gaccaataat gccatcaaaa aaagacacac aacaccgttc atataagcaa
    54601 tcatcattta gctgataatc ttcaattttt gccaaaatct cagaagcttt atcatactct
    54661 tgtcgctgaa taaacaaaat caacatattg acaaacatcc gaatcgattc attcccataa
    54721 taccttaggg tattgtattt atcgaggttc aaaataactt ttgaaaacac catctcaata
    54781 aagcaagact caaaaataaa catacaatta ttaaaaagca cagtctcata gtgactccaa
    54841 gtttcaatat taataagata gtttgacaaa taagtccgct ctgtcagaca gtcttcctca
    54901 gtaagagttg ctaataacac cttgaccaag gcaaaaaggt tcttttcctt tgttgactta
    54961 ctatctttat aagaatcaag gatatgattt aaaccttcta tgtttcggca ttcaaaataa
    55021 tgttttgccg tatccatatc aataaattct ttgtattgtt taaagttatt actaataaag
    55081 agaaactcat caacattgac attcaagttg tctaagatat aaagtaactt tttcgaagaa
    55141 atgtcaagat tatttttaat aaaacgataa taggtttgcc tagtgagata atcaccacaa
    55201 acttgtttaa tcgaaatgtt ttttgaatgc ctaatgaatt caacggtttc accaatttcc
    55261 atatgtcaag ccttcctagt tgatgtcaaa aatacgttac gcatgtacct aatacgtaac
    55321 aagttgaatg tttcggatga tagtcgctta tgataggtgc ataaggtcaa taaccagatg
    55381 cgatagtttt atcaactgtc atatgttaaa cctttctaat ctaatagatg ttaaaaatac
    55441 gttacgtgtg tacctaatac gtaacaaaat aatgggttag caaaataagc agctatgata
    55501 tagccataag gttaaaagga ggcgcctact atgtggttat tgttactatt tttgtagttc
    55561 cttttgcaaa atggtagtag ataaaaatga gaagtcaata aaagcattga caaacaaaag
    55621 aatcattttg tttatattat cagtattttt aagtttcttg tcaactgaaa tgagcatcta
    55681 ctagccacaa tagtaactaa aaggctaatt gtcagtaaaa atgagataaa tcagccagga
    55741 aaaagcctac gagtcaacta atggacaacc caaaaaaaga caaaggactt tcacttggtg
    55801 ttgtggaccc tatcacgatc cttatcacag aatcagttat taacaaaatg agaacggtcc
    55861 catatcatct aaaaaaactg tcaagcacaa cctattgttt gctagctgat cttgcctatc
    55921 agtctgccta gcagatacgg cagaagataa gaaatcaacc aaaagatgat aagtgcaacg
    55981 actcatagcg tcttttgacc aaaaccaatt aggctcacat gagatgagaa taattgggtt
    56041 gggttgtcag tgtcaactaa ccgtgttatt gtctattacc attcatggta tcagcgacat
    56101 cgtatgataa tcatacgatt cagctaagta aggaggtgtg tccaatgtac cgttaaaagc
    56161 aaatgcagta gattaactta ttttgaaaga ggtataaaaa aaatgaataa aaagaaatta
    56221 ggtatcagat tattaagtct tttagcatta ggtggatttg ttcttgctaa cccagtattt
    56281 gccgatcaaa actttgctcg taacgaaaaa gaagcaaaag atagcgctat cacatttatc
    56341 caaaaatcag cagctatcaa agcaggtgca cgaagcgcag aagatattaa gcttgacaaa
    56401 gttaacttag gtggagaact ttctggctct aatatgtatg tttacaatat ttctactgga
    56461 ggatttgtta tcgtttcagg agataaacgt tctccagaaa ttctaggata ctctaccagc
    56521 ggatcatttg acgttaacgg taaagaaaac attgcttcct tcatggaaag ttatgtcgaa
    56581 caaatcaaag aaaacaaaaa attagacact acttatgctg gtaccgctga gattaaacaa
    56641 ccagttgtta aatctctcct tgattcaaaa ggcattcatt acaaccaagg taacccttac
    56701 aacctattga cacctgttat tgaaaaagta aaaccaggtg aacaatcttt tgtaggtcaa
    56761 catgcagcta caggatgtgt tgctactgca actgctcaaa ttatgaaata tcataattac
    56821 cctaataaag ggttgaaaga ctacacttac acactaagct caaataaccc atatttcaac
    56881 catcctaaga acttgtttgc agctatctct actagacaat acaactggaa caacatctta
    56941 cctacttata gcggaagaga atctaacgtt caaaaaatgg cgatttcaga attgatggct
    57001 gatgttggta tttcagtaga catggattat ggtccatcta gtggttctgc aggtagctct
    57061 cgtgttcaaa gagccttgaa agaaaacttt ggctacaacc aatctgttca ccaaatcaac
    57121 cgtggcgact ttagcaaaca agattgggaa gcacaaattg acaaagaatt atctcaaaac
    57181 caaccagtat actaccaagg tgtcggtaaa gtaggcggac atgcctttgt tatcgatggt
    57241 gctgacggac gtaacttcta ccatgttaac tggggttggg gtggagtctc tgacggcttc
    57301 ttccgtcttg acgcactaaa cccttcagct cttggtactg gtggcggcgc aggcggcttc
    57361 aacggttacc aaagtgctgt tgtaggcatc aaaccttagt atggaaatgc atttcgttag
    57421 aacagaacct gaggcacgcc gcatagctga aaccttttgt gccgaaaaca cacaaacaaa
    57481 aacccctatg cgcgtgcagc agttaagcta tccatcagac acagatcact caggtggtga
    57541 gctatacatc tatgctttgt ctcctgctgg atttatcatc gtatcaggag ataccagagc
    57601 gcacaccatt ttaggctatt cttttgataa taacctggac ctcaaccatg ataatgtcag
    57661 aagtatggta gaagcttacc aaaaacaaat caactctttg gattgatcgg tcaaagtagc
    57721 ttttgccccc tcaccactcg tttaagacga ccgtgtttct aaaataccac taccttttaa
    57781 gtttcacgcc ggcctaagca agtgtttgtc tcatggagca gactgtttcc atcgaaaagt
    57841 cacccattga taaaggaggt catcaacgcc tactaatgat gacttcccta agatgccaag
    57901 gtaaaggact gagttctgta gagatgggac tcagtccttt tacgattttt gggagttatt
    57961 ctagccatac cgtggcaaga aaacaacact caaaaatagc caacgtcagt ttctgttcat
    58021 acatgactac agccaaacac cacaacctaa cctatcctaa acacagtggc tttctaacga
    58081 acagtcacaa cgagcagcaa caccaaaaag taagattaag ctacgtttaa gactaatgat
    58141 ataagataac agtaattatt aatgagaaaa gaggctcaaa aggacatgaa acaaatgaat
    58201 aaactcatta caggagtggt aacgctggcg acagtcgtga ccttatcagc ttgtcaatca
    58261 tcacacaaca acaccaaact cgtctcaatg aaaggagaca ccatcactgt tagtgacttc
    58321 tacaatgaga ccaaaaacac agaactcgca caaaaagcca tgttaagctt ggtgattagc
    58381 cgcgtttttg agacacaata tgccaacaaa gtctctgaca aagaggttga aaaagcctat
    58441 aaacaaaccg cagaccaata cggtacatcc tttaagacag tcctagcaca atcaggctta
    58501 acaccagaaa cctataaaaa acaaattcgc ctcacaaaat tagtcgaata tgccgtcaaa
    58561 gaacaagcca aaaacgaaac catctcaaaa aaagactacc gtcaggccta tgacgcttat
    58621 accccaacca tgaccgcaga aatcatgcag tttgaaaaag aagaggatgc caaagcagca
    58681 cttgaagctg tcaaagctga aggggcagac tttgcagcta ttgccaaaga aaaaaccatt
    58741 gcagccgata aaaaaacaac ctacacgttt gactcaggcg aaacaaccct accagcagaa
    58801 gtagttagag ctgcatcagg cctcaaagaa gggaacagat cagaaatcat cacagcgctt
    58861 gatccagcca cctcaaaacg cacctaccat atcatcaaag tcaccaaaaa agcaactaaa
    58921 aaagcagact ggaaagcgta ccaaaaacgc ttgaaagaca tcatcgtgac tggcaaatta
    58981 aaagaccctg acttccaaaa caaagtcatc gctaaagctc ttgataaagc aaatgtcaaa
    59041 atcaaagaca aagcatttgc caatatctta gcccagtttg caaaacctaa ccaaaaacaa
    59101 cctgcccaaa aatagagggt tgccagttta tttccctaag gttgcttaaa aagacagccc
    59161 tagccatcac tccccttttc aaaacaccga taatggaaac gtgtcctata ctgatggtct
    59221 agggcaatgt cataaagcta aagactctat cccaataagg atagagtctt taatgtttta
    59281 aaaccagtca gcaaaaaaat tcacgctaag tctagaatga ggcttagcgt gaatggtatc
    59341 agtgatagtc cgttaaacta gctgattggg agtcagaaac tctccttacc caatctgact
    59401 atacccccag taccagaggt gtctcatcgc agaagtagca atcagataat cgtctgataa
    59461 gctcttgtcc tcaagcaact gatgagtctc gtcgcacggg ccatgagctg ccggtggctg
    59521 atagccagta ggctcatcag ccaaaacttt tttggagtaa cgctttggtc tcagacggcg
    59581 ccagagggtt gagagagggg tgagaaaaaa acgatggatc atcttggcct ccttgtctaa
    59641 tgatagtcac cttaacagac aaaagtgtca ataatgtcat tttaagaaga aaaagattaa
    59701 aaatcaaggt cacaaacttt cctttgttaa aaaaacgaga ccctaacaaa gaccctaaat
    59761 acccctcgca cctgagcttc tccttgcgca gctatcttgc gcactcagtt gtctccaggc
    59821 tagtgatagc gataccatta ttgtcctata ggctatcgcg acaacccctt ttttcgcttc
    59881 aacgaggccg acacagctgt catgacagaa aaaatacctc tgcctatcta agggaccatt
    59941 aatttttctc tcaccaaaaa actgatttta gaaacgaaaa agcatggtgt ataataaagt
    60001 tcggaacaat tatgacataa taatgaaagt aaggttaact aaacattgac aaattgtaag
    60061 tataaactta gaaagttatc tgtagggctc gtctccgtcg gaacgatgct gatagctccg
    60121 acagttttag gacaggaggt tagtactggt gctagcagta ctgagacgag tgctagtact
    60181 aatactagta ccgctagcgc tggtaccagt acgagtgaga cagctgccag cggaactggg
    60241 agtgaagcag ctgtagtatc tagcgaagga agtcagagtt cagaatctgg acaagcctca
    60301 acacaacctc aagcacagac tttagaacaa tcagcagcaa cgtcgccatc atcgaactct
    60361 tctattagta gtagtgaaga taaagctcct aaggcagcaa gcactaaatc atcttcagca
    60421 actgtggcta gctctagtaa tggtagcaat caaggtgctg gtgctgaaga tgcaccacag
    60481 atgatggacg tggaacggta tacagttgat agggaaaaaa cagagctaaa tattaaagac
    60541 ggtaagactc caaaaactag gaatagtgtc gataaagata caaagcttat tagaaaccgc
    60601 gatggcaaac agcgtgatat tgttgatatc aagcgtgaag taaaagataa tggcgacgga
    60661 accttagatg taaccttaaa agtaacacct aaagaaattg ataaaggtgc cgatgttatg
    60721 gcccttttag atgtctctaa aaagatgacg gatgctgatt ttaaaaacgc taaggataag
    60781 atcaagaaat tagtcacaac cttaacgagt aaatcagcga gtaactcaga taatgatgag
    60841 cataaacata attctcgaaa ttcggttcgt ctgatgacct tttaccgtga gattagcaac
    60901 ccaattgata tatcgggaaa aactgatgct gaacttgata aattattaga cgatcttcga
    60961 gtaaaagcta aagctaatta tgactggggg gttgatttac aaggcgctat ccacaaagct
    61021 cgggaaattt ttaataagga aaaagagtca aaaaaacgcc agcatatcgt cttgttctct
    61081 caaggcgagt cgacttttag ttatgatatt caaaagagtg agaaagagaa aagtagcaat
    61141 ttatctcgta taaacgaaaa aattacctcc tctaatccgc tgttgccctg gccaccaatt
    61201 tttgatcata cgcatcaaaa tggagatatg attaatgatg ttagagcttt aattgcattt
    61261 gctagtaagc taggtataaa gaatttatca tggattgaaa catcacttag tgcgttaagc
    61321 gtaggaagta acttgcttgg ttctgttttt ggtggaggtg gactaggcga gtacctaact
    61381 ttaaaagaat atgattcaca aaaattaaat gaagaccaat ttgattatac taaacgcgta
    61441 ggcgaaggat atcattatca tagtttttca aatagggaat ctgaggataa aatgcctctt
    61501 gaaacagaaa ttaaaaaagc tttagaagct gctttaccaa aattcgaaga aaataactgg
    61561 tttaccaaag tactaaaata ctttggattg aaagataaag ctgaacaagc caaacttgat
    61621 gtaatcatga aagtgattaa aagtgtcttc tacaagcgcc agtaccacta ttataatcac
    61681 aatctctcag cgatagccga ggctaagatg gcgcagaaag agggcattac cttctattcc
    61741 gttgatgtta ctgctttaaa caccgctaga agagtgaagc gacaaacagc agtgctaaag
    61801 aacactaagg aagaagaacg taataagaag tttgatgagt atctgaaaaa gatgtctgaa
    61861 ggcggtaacg cattttttaa cgatgtggat aaggcagata agttcaaaga cactttaaca
    61921 aaacttacga ttaaagacga gtttgacggc aaggttagtg tcgataaaac ttcatatcaa
    61981 tccagtagtg caatcaacta ttctgaagct agtaatagtt tttggcgtac taccaaagaa
    62041 agtctcactt ggaccatttc caaagagcag ttgaaaaaag cctttgaaga tggaaaaccg
    62101 ttaaccttca cctataagct taaagttgat aacaacaagt ttaaaacagc tctcgaggaa
    62161 aataataaga agaagagaac aaaacgttct acacctacag aaaatgaaaa ctctgtcaca
    62221 gaaaaaatca tttcaaatac tactacctac gaaattaatg aacaaaaagt tgaagggaac
    62281 aaacttggtg atgttagtct gacatacagt aaattcaagg ttcctgtacc acagattgat
    62341 gggcatgtta ttgagccaca agcaccgaca ttacctaagt tacctcctgt tattgagcat
    62401 ggccctaact ttgagtatga ggaagaaaca ggttatcagt taccacttaa acatggcagc
    62461 aatgcaccag acacacaagt gacaattgaa gaagacacag ttcctcaacg tccagatatc
    62521 cttgtaggcg gtcagagtgg accggttgac atcaccgaag atacccaacc aggtatgtca
    62581 ggctcaaatg acgcgacagt tgtcgaggaa gacacagcac ctaaacgtcc agatgtcctt
    62641 gttggtggtc aaagtgagcc aatcgatatc actgaagata cccaaccaag tgtgtcaggc
    62701 tcaaatgacg cgacagttgt cgaggaagac acagtacctc aacgtccaga tatccttgtt
    62761 ggcggtcaaa gtgatccaat cgatatcacc gaagataccc aaccaggtat gtcaggctca
    62821 aatgacgcta ctgttatcga agaagatacg aaaccaaaac gcttcttcca ctttgataac
    62881 gagccacaag caccagaaaa acctaaagag caaccatctc tcagcttacc acaagctcca
    62941 gtctataagg cagctcatca cttgcctgca tctggagaca aacgtgaagc atcctttaca
    63001 attgttgctc taacaattat tggagctgca ggtttgctca gcaaaaaacg tcgcgacacc
    63061 gaagaaaact aaccctcgtt agtctagcga ccctaagcct ttggctttaa aaatttctca
    63121 ttatccccct caaacaccgg tacttcactg agatggcgct ttaaaaaggc gctagtctca
    63181 aatcaggtat cccctacggt tgagggggat taatgggtat cacaacaagt aaaaaggtat
    63241 aatgatgaaa tatgactaaa aaatttatta aatcagcagt gattctaggc ttagcaagca
    63301 taaccatcag catgacgcaa cccgttgaag ccatttcaaa tcgcttagaa tatcaagaac
    63361 aatataaacg tgtcaaagaa gaactagacg cattaaaaga aaagagccca aaattaggag
    63421 aacgttccct ttattttaga ttggtgaata gatttctagg ttggtttggc gtagtctggg
    63481 acccactcgc aatcaaagaa accgatcacc tgttggacct catcggacaa gtcaattact
    63541 tggtgagtaa caccgagttt caagcccctg agtatcaaaa ggctaaaaac aaactagact
    63601 tagctgtagc aggctatgac ctctacctag ctagagagaa aaaacgtggt gatgctcctg
    63661 attaccaagt gacccagcta acgctaggac agctcgaaac tatcaaaacg aagatacaag
    63721 aggccctgaa aacatttgca caagaggtca aaacaatcca acaaaaaacc ccagacctat
    63781 ttccccatgc ttatgtttta aacgctaaga ccagtgctat cgatggtaaa gcgcaaccct
    63841 ttttagtgaa tggttactac gatgtctttt ataagcagtt tttattcact aataaaaatg
    63901 acgggtataa ctataataaa gaagcagaca atctgacctc gtatcaaaat ttgattaaag
    63961 agctattaga cttaaaagct gagatcgatc ggttaacaaa agacgatcct aaaaacgatc
    64021 gtttagagac gttaatgtgg caatcaaagt tcaccttatc tgactttcgg agaacttcac
    64081 gccttagcgc ctactataca caaggcgatt atgtcaaaac ggtggtccta tctgaagaag
    64141 ctcccaactt gagaacagca aaagaaaagg tcaagacctt aaaagggatc ttgcatgatt
    64201 actatgttga aactaataag ccagaaaaag ctaaagagta tcgtgtcgag gacgagacct
    64261 tgccgtctca acctgaggct cctgttacac ctgaagctcc tgctcctagt cctgttcctg
    64321 gtcaaaaacc agcagaagct gagagatcgt cacaagctac ggagccagct aacccgtcaa
    64381 aagagaattc ctcaacagac gcatctcagg gatctcacga tagtgaaaac ccagcgacag
    64441 acagtccttc tcaacctcag gcaccagacc aaggcaatca ccaaagccaa gtgcctaacg
    64501 acaagcctca aacggacaag acggacactc ctaacgtccc agcccctcca cagaacactc
    64561 ctaacgcccc agccatccca caggacaccc caaaagtgcc tgaggctggt ggtcagagtg
    64621 gccccgctgg gaacgctgag gagaaagctc ctgactctgg acctaaagcg tctgatcaaa
    64681 gctcgtctaa agagtcccca tccgttggcg aagacacgac ccctaatcag ccagatgtcc
    64741 ttgtaggtgg tcaaagtgag ccaattgata tcaccgaaga cacaatcact gacgctcctc
    64801 cgacagtatc tgggcacaac gcatcaacac agccccaatc tgtggtcgaa gacacggcac
    64861 ctcagcgccc agatgttctt gtaggtggtc aaagcgagcc aatcgatatc acccaagata
    64921 cccaaccggg catgtcaggc tctaatgacg ctaccgttat caatgaagat acaaaaccaa
    64981 aacgcgtctt ccactttgac aacaaagagc cacaagcatc agaaaaagcg gcagagcaaa
    65041 aacttgctcc tcatgatagc cacacaactc ctcaagcttc agatgataca gcagcccctc
    65101 acttgcctgc atctggagat aagcctgaaa aagcgtttgc acttgctgcc ttgacaatgg
    65161 tcgcagctgc agggctactc agcaaaaaac gtcgcaacac cgacaaaaac tagtcgctgt
    65221 tagacacaac gcatcactaa ggccagtgca tttgcactgg ccttagttag gtttttatca
    65281 aatagaggtt ttttcatgac aattgctagc gtttcggtgt aaaataaagg taataaccag
    65341 caaaggagca acccatgaca aagcaagatc agttaattgt tgaaaaaatg gaacaaacct
    65401 atgagacctt tagcccaaag ctagcaaatc tcatagaagc cctagatgcc tttaaagagc
    65461 attatgagga atatgccaca cttagaaatt tttacagtag tgacgaatgg tttcggcttg
    65521 caaaccaacc ttgggatgat atcccgtgcg gtgtcctatc tgaagacctc ctctttgata
    65581 tgatcggtga ccataaccaa ctactagctg atatcttaga cttggcccct atcatgtaca
    65641 agcatatgta accatcgcta acgtcacaga tactacgatg acaggacaaa aaaagcttga
    65701 aaatctcaaa aaaaaacaga taaaaaacag ataatcgtta gaaaaacagt agatgcgtct
    65761 gttaaactaa aggtatcaaa acaaaaggag catttcatca tgaaattcaa aaaagtatta
    65821 gtcattcctg cattagcatt agcagcaaca tgctttttaa cagcttgtgg cacaaaaaaa
    65881 gattctaaaa aagaagaagt taaagaaatc aaaatgtctg acatcaaaga cgacgcagtt
    65941 agcaaaaaaa caaaagtggt tgatggcgaa gaagttacag aatacaccac aaaagatgga
    66001 aacgtgattc agattcctgc aggaaacgaa gaaggcatgg aatcaaaaga tgctggtgga
    66061 tcaggcgctc ctgctaaaaa ctaagggtta caacaaagag cataaggtga ctcgcaaacg
    66121 ttacgcatga catgaggcag tctgcctaag atggtaactc acttatcata atgcctaaat
    66181 agccaaacac actaactcgc aaatgtacaa aatcatatac ccgtgcttat gtgagcgaac
    66241 ggttatatga tttttgtttt ccaaaaacaa gaaaagaacg ctaaaatagc cagtttgtcg
    66301 tgtttcaagc tacgaaaaag gaagtgctaa aatgaaaaaa agatccatta tacctgtcat
    66361 cttactgttg gtcatcggat taatcgttgg tgttaagcgt catcatgact agtaggtctt
    66421 gctagtgacc gttcaaaagg gataacgttg gtatggtata accataaaaa tcgtataaaa
    66481 agaaaaaata gctattgagg agaaagtatg tttcagttaa gaaaaaaaat gacgcgcaaa
    66541 caattagcct tgttgagtgc tggagtgttg acctgtgtgg ttggtggtag ctacttgata
    66601 atgaaccatc aacaacaaga aattgtctct agtgtcaaca aagtaaaagc cttaaccata
    66661 aaagaagcca tggaacaagg aaaagatatc agcttgacct tagctggcga agtaacagct
    66721 aacaacagca gcaaagtcaa aatcgactca agtaaaggag aagtcaaaga tgtctttgtc
    66781 aaaaaaggcg atgttgtcaa agtaggacaa cccttgttta gctatgaaac gtcacaacgg
    66841 ttaacggctc aaagttcaga atttgatgtt caaaccaaag ccaatcaact ccaagttgct
    66901 aaaaccaatg cagcattgaa gtgggaaacc tacaatcgca aggtcaatga aatcaacacc
    66961 ctaaaatctc gctacaacac tgcaccagat gagagcttac tagagcaaat tcgcagcgca
    67021 gaagacagtg tatcccaagc actaagcgat gccaaaacag cagatagcga tgtcaaaacc
    67081 gctcaaatcg aactcgataa agctaatgct actgccgcaa cggaaaaagg taaactagag
    67141 tatgacaccg ttaagtcaga caccgcagga accattgtta gtctaaatac tgatttgcca
    67201 aatcaatcaa aatccaaaaa agaaaatgaa acttttatgg aaattatcga caaatcaaaa
    67261 atgttagtca aaggtaacat cagtgaattt gaccgtgaca agttaaaaat cggtcaaaaa
    67321 gtcgaagtga ttgaccgcaa agacaactct aaaaaatgga ctggaaaagt aacccaagtt
    67381 ggcaacctca aagcagagga aaaaggccaa ggtcaaggcc aaggtggcaa tgaccaacaa
    67441 gataatccaa accaagcaaa attcccttat gttatcgaac ttgaccaatc agacaagcag
    67501 ccactcattg gttcacacac ctatgttaat gtgctcaaca atgttccaga agctggcaag
    67561 atcgtattga aagaaacctt tacaatggca gaaaatggaa aaacctatgt gtggaaagtt
    67621 gataaaaaca aggtcaaaaa acaagaaatc aagactaagc ccttctcaaa aggttatgtt
    67681 gaggtgacaa gtggcttgac tatgcaagat aagattgctc agccgcttcc tggcatgaaa
    67741 gacggtatgg aggtaggaag tattgttaaa ccttaaagat attcgaaaaa gctatcatct
    67801 tgggactgaa gaatttgcga ttttaaaagg aatcgattta gaagttaacg agggtgactt
    67861 tttagccatc atgggaccat caggttcggg aaagtcaaca ttgatgaata tcattgggtg
    67921 tttagataag cctggctctg gctcatatgc cattgaaggc agagacgtgt catccttatc
    67981 tgataatgaa cttgctgatt tgcgtaatca aaaaatcggc tttgtttttc aaaactttaa
    68041 cctgatgccc aagctaacag cttgtcaaaa tgtcgaattg cccttgactt atatgaatgt
    68101 tcctaaaaaa gagcgtcgca aacgagccct agagatgtta aagctagtag gattagaaga
    68161 acgtagtgaa tttaaaccga tggagctatc tggtgggcaa aaacagcgtg tagcgattgc
    68221 aagagcttta gtcactaacc cgagttttat ccttggtgat gagccaacag gtgcactaga
    68281 cacaaaaacc agcgtccaaa tcatggacct atttaaacaa ttcaatgata acggcaaaac
    68341 gattattatc atcacacacg agcctgaagt agctgcttta tgcaaaaaga cggtgatcct
    68401 aagagatggt aatatagaac attccgatat agagtaaagg gggaaaataa tggaagatat
    68461 tattgcagct ttaagctcta tattatcaca caagatgcgg tcaattttga caatgctagg
    68521 gattattatc ggtattggtg ccattatcgc aatcttttca atcatcgaag gtaataccga
    68581 aaataccaaa agacagttga ttggtggcag caataacact attaacattg tgtttaacaa
    68641 aaaaagtagt attgacccta aatttccaga caagtcaaat gctaaaaaac cagactatct
    68701 tccttttatg gctgaagaag agcttagcaa aatacagcaa gtcaaaggcg tcaaaaatgc
    68761 cctgatctca tatggtattg atgacaaagt ctaccatcta ggccaaaaat catctgcaaa
    68821 gatttctgcc atcacaaaaa acgtagctga agtgagacgc atgacattta ttaaaggcag
    68881 tgacttttca gacaaagact ttattgacca aaaacaggtg atttaccttg aaaaaagctt
    68941 gtatgagtca ctatttccaa aagacgatgg ccttggaaaa tttgtcgaag tgatgggaaa
    69001 tcccttcagg gtcattgggg tgtttgaatc aaaagaacaa agtggcctca caagtgggac
    69061 tgagaaaatt gcttatatcc cgctacatca gtggtataat attaatggtg tagtggatgc
    69121 gacacctgag atcacgatcc agacctatcg cgcagatgac ctcaaacctg tagcaaagcg
    69181 tgtcagtgat atgctcaatc aaacgatccc taaatcagat tatatgtttg gtgtcatgaa
    69241 ccttaaagaa tttgaacgcc aactagacaa cctaaacaaa tctaattttg tgttattagc
    69301 tggtattgct agtatctctc ttattgtagg tggtattggt gtgatgaaca ttatgctagt
    69361 atcagttacc gaaagaacga gagaaattgg gatcaaaaaa gcgcttggcg ctagacgtaa
    69421 gcttatttta aaacaatttt taattgaagc ggtcattcta accttgctag gtggtgtgat
    69481 aggggttatt tcagggatgg tatcaggatt gatcatcaca agatcactcg aatatcccta
    69541 tatcctatcc ttgttctcag ttgtcttgag tttagcgttt tgttgtatta tagggattgt
    69601 ctttggacta ttaccagcca ttaaagcgtc taagctagac ccaattgaag cattgcgatt
    69661 tgaataatca accaaacaag cataaaagtc aaaaaggatg accctttgat gtcatcctta
    69721 gctgcttgat acactaaacg ccaatctaga aagggaagtt atgtttaaaa tattagtagt
    69781 tgaagatgat gataccatca gtcaagtgat ttgtgagttt ttaaaagcga ataactatga
    69841 tccagattgt gtatttgatg gccaagctgc cttagacaag tggcagacaa cgtcttatga
    69901 tctgattatt ttagacatca tgctcccatc cttaagtggc ttagaagtgc taaaaacaat
    69961 tcgaaagaca tcagatgtcc ctattattat gctaacggca ttagatgacg agtacacaca
    70021 gcttgtaagc ttcaaccatt tgattagtga ttatgtgact aaaccatttt caccactcat
    70081 tttaatcaaa cgtatcgaaa atgtattacg cgtctctact ccagatgaaa agcgccaaat
    70141 cggagactta cttgttgatg agaccgaaca tagcgtctat tggcaaggaa cattggtcaa
    70201 actaaccaaa aaagagtatg acattattga ttatttagca aaacgtcacc aaaagattgt
    70261 caccagagac caattgatgg atgacatatg gggctattct gaactggaca cgcgtgtcct
    70321 tgacaaccac atcaagaacc ttcgtaaaaa aatgacgggt attcccctca agacaattac
    70381 aggcatgggc tatttattgg gagagcgtga gtgagactaa tcaaaaaaac ctttttggtc
    70441 atcaatggct taattattgt agtggttacc tctattttgt tagtgcttta ttttgcaatg
    70501 ccaatttatt acaccaaagt caaagacaaa gaagttaagc gtgaatttga ccaaacaagc
    70561 aagcaaatca aaggcaaaac ggtaaccgag attagagata tcctaacaaa aaaaatcaac
    70621 aaagataata tttggtacag cttagtagac agtgataacc aactgctcta tccttccttg
    70681 cagctgttag acggcgtcag tgaaagcaaa gacagtcaaa atgttaatat cgtcaccacc
    70741 tttgacaact cttattctaa tgttaaggtg atgagtcaaa aagtaaccct acgcgatgga
    70801 aaaaagatga cattgcttgg gcaatcttca cttcaacccg taacagatgc tagcaaagtt
    70861 ctccttgacc tctacccgtc actattgatt ttttcagtga ccgtagggag tattgtcgcc
    70921 tacttatata gtaggacttc tagccggcgt atccttagca tgtcacaaac cgccaaaaaa
    70981 atggtcaacc tagaaccaaa cttaacgtgt accattcacg gcaaagatga aatagcaatg
    71041 ttggctagtg atattaaccg tttgtatgcg agtctttcaa cgagcatcaa atccttgcaa
    71101 aaagagtatg aaaaagcttc tgattcagaa agagagaaat ctgagttttt acgcatgaca
    71161 tcgcacgagc taaagacacc aatcaccagt gttatcggaa tgattgatgg aatgctctat
    71221 aatgttggtg attttgcaga tcgtgacaaa tatttgcgaa aatgccgaga cgtccttgaa
    71281 gggcaagctc aactggttca atccatttta tctttatcta agattgaaac cctagcttct
    71341 caaaatcaag aactgttttc actaaaaagc agcctagaag aagaaatgga agtctttctc
    71401 gtcttatcag aactaaaaca cctcaaagtg actatcaatc tcgaagaaca attcgtcaaa
    71461 gccaataaag tatacctact aaaggcgatt aaaaatatta ttgacaatgc ctttcactat
    71521 accaaatcag gcggccaagt gatgattcaa ctaaaagaca accaactagt gattaaaaat
    71581 gaagcagaga cattattgac acaacagcag atgaaacagt tattccaacc gttttatcga
    71641 ccagattata gtcgtaacag gaaagacggt ggtacaggat tggggctgtt tatcacccac
    71701 cagattcttg atcagcatca tctggcttat cgctttgttg ttcttgatca aagatggatg
    71761 gtatttacga ttgattttcc atcccatcat gacgattgat ccacactagt aggacgacaa
    71821 aagccatcaa tcactaacca agccttgacc tttacccatc attgtttagg caagtataaa
    71881 aaagaggaga gaaatcatat atgaagaaaa ggaaattgtt agcagtaaca ctattaagta
    71941 ccatactctt aaacagtgca gtgccattag ttgttgctga cacctccttg cgtaataaca
    72001 catcatccac tgatcagcct actacagcag atactgatac ggatgacgag agtgaaacag
    72061 caaaaaaaga caaaaaaagc aaggaaacag cgtcgcagca cgacacccaa aaagaccata
    72121 agtcatcaca cactcaccca acccctcctt caaatgatac taagcagacc gatcaggcat
    72181 catctgaagc tactgacaaa ccaaataaag acaaaaacga caccaagcaa ccagacagca
    72241 gtgatcaatc caccccatct cccaaagacc agtcgtctca aaaagagtca caaaacaaag
    72301 atggccgacc taccccatca cctgatcagc aaaaagatca gacacctgat aaaacaccag
    72361 aaaaatcagc tgataaaacc cctgaaaaag gaccagaaaa agcaactgaa aaaaccccag
    72421 agccaaatcg tgacgctcca aaacccatcc aacctccttt agcagcagca gctcctgtct
    72481 ttgcaccgtg gagagaaagt gacaaagacc tgagcaagct aaaaccaagc agtcgctcat
    72541 cagcggctta cgtgagacac tggacaggtg actctgccta cactcacaac ctgttgtcac
    72601 gccgttatgg gattactgct gaacagctag atggtttttt gaacagtcta ggtattcact
    72661 atgataaaga acgcttaaac gggaagcgtt tattagaatg ggaaaaacta acaggactag
    72721 acgttcgagc tatcgtagct attgcaatgg cagaaagctc actaggtact cagggagtcg
    72781 ctaaagaaaa aggagccaat atgtttggtt atggcgcctt tgacttcaac ccaaacaatg
    72841 ccaaaaaata cagcgatgag gttgctattc gtcacatggt agaagacacc atcattgcca
    72901 acaaaaacca aacctttgaa agacaagatc tcaaagcaaa aaaatggtca ctaggccagt
    72961 tggatacctt gattgatggt ggggtttact ttacagatac aagtggcagt gggcaaagac
    73021 gagcagatat catgaccaaa ctagaccaat ggatagatga tcatggaagc acacctgaga
    73081 ttccagaaca tctcaagata acttccggga cacaatttag cgaagtgccc gtaggttata
    73141 aaagaagtca gccacaaaac gttttgacct acaagtcaga gacctacagc tttggccaat
    73201 gcacttggta cgcctataat cgtgtcaaag agttaggtta tcaagtcgat aggtacatgg
    73261 gtaacggtgg cgactggcag cgcaagccag gttttgtgac cacccataaa cctaaagtag
    73321 gctatgtcgt ctcatttgca ccaggccaag caggagcaga tgcaacctat ggtcacgttg
    73381 ctgttgtaga gcaaatcaaa gaagatggtt ctatcttaat ttcagagtca aatgttatgg
    73441 gactaggcac catttcctat cggacgttca cagctgagca ggctagtttg ttgacctatg
    73501 ttgtagggga caaactccca cgaccataaa cccacaacca agaagctacc tgcctgttga
    73561 ccaatctcaa aaagagatag cgtcaactta gaatgaggtt catcagatga taaagaaagt
    73621 gaccaccccg agtcaaaaaa taaaaaaacg tgttcgtaat ggctacctct taaaactagg
    73681 tacagcctgt ttactcttaa gtatactcag ttatggcatt ggtcttttgg gccagcctag
    73741 catggaaaat acgtttatgg gaatagcgag tgttgccatg ttaggaagtg tctgtttctt
    73801 tattatcttt gctctaaatc gtatctttga cgcactagag gacaatttgc gagattagag
    73861 taataggtca aataatcttc caataattta gagcataagc taagttattg gaagtttttt
    73921 tgtcttttga gagccatgat gccatcaaaa agccaaacac tccttatgat gtgagagtca
    73981 catgactaaa attaaaagca aaatagaccc caaagccctg ttttctcaaa acagccaacg
    74041 aaaaaagaca agttaaccag ttcataaaaa acatcgtaga aacattttca agagctaatg
    74101 ttggtgtaaa ttgactgaag tatgatagaa tttttaatga atgtgacaat aatgtcacag
    74161 ataaaaaccg aaaacagcaa aaataattaa attgaatata aaaaagttaa ttaagtgagt
    74221 gataataaaa aaatgaagtt caaaaaatat aaataaaata aaacaccaga actgctaaaa
    74281 gcagtgaatg acataaatgt cgctaaaaaa atagatagaa gagtctaaaa tttcagtttt
    74341 aggtcacgtt ttatgaatag aaatcaggat gatgacccta agttgtgatt tgcgcgtcat
    74401 tgactgcttt cctcgtttta atgaaagaat ttgaaagata ttttatggta tacttttcct
    74461 taatatggtt catacggact tgataaatct atttacaaaa ataataaaaa attaagataa
    74521 ggacatgaag ttaatgcatg taagtaaatt atttactagc caacaatgga gagaattgaa
    74581 actgatctca tatttaacag aaaactccaa tgctatcgga gttaaagata aggagcttag
    74641 caaggctctc aatatttcca tgttaacatt acagtcatgc ctcactaata tgcagtttat
    74701 gaaagaagta ggtggaatta cctacaaaga cggctatatt aacatttggt atcaccagtg
    74761 ttgtgggcta caagaggtct atcaaaaagc tcttcgagaa tcgccttctt tgaaattact
    74821 agagcttttg tttttcagag actttagttc cttagaagag ttagctgaag agctgtttgt
    74881 cagcttatct accctcaaac gcctcatcaa aaaaaccaac acttacttgt ctcatacctt
    74941 tgctattagt atcgtgacaa gtccagtgca ggtatcagga gatgagaggc aaatccgcct
    75001 gttttacctc aagtattttt cagaagccta taaaatctca gaatggccat ttggggacat
    75061 tttaaacctg aaagactgtg aacgcctatt gagcttattg atcaaagaag tagatgtcaa
    75121 agttcatttt acactatttc agcacttaaa aatcttaagt ggtgtcaact tgatccgtta
    75181 ctacaagggg tattcttgca gttataacaa taaaaaaacc agtcaccgtt tttctcaact
    75241 catccaacat tcctcagaaa tccaggatct ttctcgcttg ttctacctca aatttgggtt
    75301 acatctcgac gagtatacca tagctgaaat gttttctaac catcttaacg acaagctaga
    75361 aattggctgt gcttttgaga ttatcaatca agacccaaca tcaggaggca gacaagtaac
    75421 caactggatc catctattag atgagatgga aatcaagctc aatctcagca tcaccaataa
    75481 atacgaagtt gctgtaaccc tccataacgc ttcggtgtta aacgaagaag acattacagc
    75541 caattatttg ctgtttgatt ataaaaagag ttacctgaac ttttaccaaa aagaacaccc
    75601 tcgcatttat gaggcctttg tgacaagtgt cgagaagctg atgcaggcag ataatgctca
    75661 agttagcaaa gaattgatca atcagctcac ttattgtttc tttattacct gggaaaatag
    75721 tttcttaaaa gtaaatcaaa gagatgaaaa agtaagactc ctagtgatag agagaagtta
    75781 caatagtgtc ggtaattttt tgaaaaagta catcggtgag tttttcagca ttactaattt
    75841 tgatgagtta gattgtttga caattgatct agtagagatt gaaaaacagt acgatgttat
    75901 cgtgacagat gttatggtgg gtaaaagcga agagttagag attttctttt tctacaagat
    75961 gatcccagaa gctattattg atagattaaa cgaatttttg aacgttagtt tcacagataa
    76021 caacgttatg gtcaaaaacc tcgaagcccc ttccccttca aaatctcata gtgacaaaga
    76081 ggtacaaaag ccagacaatt cagttaaaca agcaacatca tcataggatt tcagacgtca
    76141 tggtaagaaa aaaagaatca tttacagccc atagaggtga ggtcaaaagc tgaaaacagc
    76201 tcaaaaaaac tgacctttac cttttggctt tttttattta gaataatttt attggagaga
    76261 tgcttaataa tttaagcaca attcttagaa attaagaaat aaggagtaaa caatgtctaa
    76321 aagaaatcca aacaagcact actcactgag aaagttaaaa acaggtactg catcagtagc
    76381 agtagctttg acagttttgg ggactggatt agcaaacaca actgatgtaa aggctgagac
    76441 cgtaggtcgc tttagtgatg aacaagttag aaaggctcgt gaaaaagcaa tcgaagacgt
    76501 gtttgatggc tatactggag ctcgttctgt ttatcaatct gggaatctgc ctaataggtt
    76561 aactcctaca aaacttagca aattaatgca acagatgtat aaggagactt tacaaaagaa
    76621 agaagaactg gataccctat ctaaagctct tacgcacact attgagaaaa agattgagtc
    76681 agaaaatgct tacaagaaag agcttggaca gctcaaagca gcggcagaag cagaagctca
    76741 aaaagcatta gatgcactaa ataacaaaaa caagcaaatc tcagacttaa ccaacgaaaa
    76801 cgcacagtta aaagaagcca ttgaaggtta tgtgcaaact atccaaaacg ctagccgtga
    76861 aatcgcagca aaacaacaag aacttgcggc tgcaaaaagc cagttagagg caaaaaatgc
    76921 tgagattgag gcattgaaac aacaagatgc ctctaagact gaggaaattg ctaaattgca
    76981 atcagaagca gcaactttag aaaaccttct aggttcagct aagcgtgagt tgactgaatt
    77041 gcaagctaag ctagatacag caactgctga aaaagcaaaa ctagaatcac aagtaacaac
    77101 cttagaaaac cttctaggtt cagctaagcg tgaattgact gatctgcaag ctaagctaga
    77161 tgcagctaac gctgaaaaag aaaagctcca atcacaagca gcagccctag aaaaacaact
    77221 agaagcaact aaaaaagagt tagctgattt acaggctaaa ttagcagcaa ccaaccaaga
    77281 aaaagaaaag ttagaagctg aagcaaaagc tcttaaggag caattagcaa aacaagctga
    77341 agagcttgct aagctaaaag cagataaagc ttcaggagct caaaaaccag atactaaacc
    77401 tggcaataaa gaggttccaa caagaccatc acaaacaaga acaaacacta ataaagctcc
    77461 tatggctcaa acaaagagac aattaccgtc aacaggcgaa gaaacaacca acccattctt
    77521 cactgcagca gcattgacag tgatcgcatc tgcaggcgta cttgccctaa aacgcaaaga
    77581 agaaaactaa gcccaaccca cactatcttt tctagcccaa gaaaaaaaca aaaaaagagg
    77641 aagaccattc ctcttttttt gaacggttaa acagtaaaaa ggtcaaaaag gtactaaagt
    77701 ctcaaaaacc tggtctttac cttttaccac ttattcttta gaatagaatt attagagaga
    77761 agtcttagaa aaatgaggct aattccctaa agatgaaaaa ataaggagca aataatggct
    77821 agaaaagata cgaataaaca gtattcgctt agaaaattaa aaacaggtac agcatcagta
    77881 gcggtcgctg tggctgtttt aggagcaggc tttgcaaacc aaacaacagt taaggcggac
    77941 agtgacaata ttaatagatc tgtctctgtc aaagataatg aaaaagaatt acataacaaa
    78001 attgcagacc ttgaagagga aagaggagaa catctagaca aaatagatga actaaaagaa
    78061 gaactaaaag caaaggagaa aagttcagaa aacgtagaac gtcactacct cagaaaacta
    78121 gatcaagaat ataaagaaca acaagaacgt caaaaaaatc tagaggaact cgaacgccaa
    78181 agtcaacgag aagtagaaaa acgttatcaa gaacaactcc aaaaacaaca acaattagaa
    78241 acagaaaagc aaatctcaga agctagtcgt aagagcctaa gccgtgacct tgaagcgtct
    78301 cgtgcagcta aaaaagacct tgaagctgag caccaaaaac tcaaagagga aaaacaaatc
    78361 tcagatgcaa gccgtcaagg cctaagccgt gaccttgaag cgtctcgcga agctaagaaa
    78421 aaagtagaag cagacttagc cgaagcaaat agcaaacttc aagcccttga aaaactaaac
    78481 aaagagcttg aagaaggtaa gaaattatca gaaaaagaaa aagctgagtt acaagcaaaa
    78541 ctagaagctg aagcaaaagc tcttaaagag caattggcta aacaagctga agaacttgcc
    78601 aaactaaaag gcaaccaaac accaaacgct aaagtagccc cacaagctaa ccgttcaaga
    78661 tcagcaatga cgcaacaaaa gagaacgtta ccgtcaacag gcgaaacagc taacccattc
    78721 tttacagcag cagctgcaac agtaatggta tctgcaggta tgcttgctct aaaacgcaaa
    78781 gaagaaaact aagctattag actgatgcta aagctaagag agaatcaaat gattctctct
    78841 ttttgagtgg ctaagtaact aacaatctca gttagaccaa aaaatgggaa tggttcaaaa
    78901 agctggcctt tactcctttt gattaaccat atataataaa aacattagga aaataatagt
    78961 aatattaagt ttgtttcctc aataaaatca aggagtagat aatggctaga caacaaacca
    79021 agaaaaatta ttcactacgg aaactaaaaa ccggtacggc ttcagtagcg gttgctttga
    79081 ccgttttggg cgcaggtttt gcaaaccaaa cagaagtaag agctgaaggg gtaaaagcga
    79141 ctacgaactt gccagaggag gctaaatatg ccgcattgaa agatgagaat actggtttac
    79201 gtggtgatca gacaaaatta gtaaaaaaac ttgaagaaga acaagagaag agcaaaaatc
    79261 tagaaaagca aaaacaagag ttagaaaacc aagcccttaa ctttcaagat gtaattgaaa
    79321 ctcaggaaaa agaaaaagaa gatctcaaaa caactttagc taaggctact aaagaaaacg
    79381 agatctcaga agctagccgt aaagggttaa gccgagactt agaagcttct cgagcagcta
    79441 aaaaagagct agaagctaag catcaaaaat tagaagcaga aaacaaaaaa ctaacagaag
    79501 ccaatcaggt ttcagaagct agtcgtaaag gtctaagtaa cgacttagaa gcttctcgag
    79561 cagctaaaaa agaactagaa gctaagtacc aaaaattaga gactgatcac caagccctag
    79621 aagctaagca ccaaaaatta gagactgatt accaagtttc agagactagc cgtaagggtc
    79681 taagccgtga ccttgaagca tcacgtgaag ctaataagaa ggttacatct gagttaacac
    79741 aagcaaaagc tcaactcgca gcgcttgaag aaagtaagaa attatcagaa aaagaaaaag
    79801 ctgagttaca agcaaaacta gatgcacaag gaaaagccct caaagaacaa ttagcaaaac
    79861 aaactgaaga gcttgcaaaa ctaagagctg aaaaagcggc aggttcaaaa acacctgcta
    79921 ccaaaccagc taataaagaa agatcaggta gagctgctca aacagctaca agacctagcc
    79981 aaaataaagg aatgagatca caattaccgt caacaggcga agcagctaac ccattcttta
    80041 cagcagcagc tgcaacagtg atggtatctg caggtatgct tgctctaaaa cgcaaagaag
    80101 aaaactaagc ctttagaact tggtttttgt aacggtgcaa tagacaaaag taagcaaggc
    80161 caaaaactga gaaagtccta aaaagctggc ctttacccct caaaattaat gttttataat
    80221 aaagatgtta gtaatataat tgataaatga gatacattta atcattatgg caaaagcaag
    80281 aaaaatagct gtatcatatg caaataaccc ctgtttgctc tttaaaaaag acgttatcct
    80341 tatttctcca cgcacagatg gacagctagg agagaatcgt ttgattctct cttttcttaa
    80401 tggtcataaa gacaaagtct ctcatcatga aaggacgaca cattgcgtaa aaaacaaaaa
    80461 ttaccatttg ataaacttgc cattgcgctc atgtctacga gcatcttgct caatgcacaa
    80521 tcagacatta aagcaaatac tgtgacagaa gacactcctg ctaccgaaca agccgtagaa
    80581 accccacaac caacagcggt ttctgaggaa gcaccatcat catcaaagga aactaacccc
    80641 ccacaaattc ctgatgacgc agaagaaaca gtagcagata aggctaatga tccagcccct
    80701 caagctcctg ctaaaactgc tgatacacca gtaacctcaa aagcgactat tagggatttg
    80761 aacgaccctt ctcaggtcaa aaccctgcag gaaaaagcag gcaagggagc tgggactgtt
    80821 gttgcagtga ttgatgctgg ttttgataaa aatcatgaag cgtggcgttt aacagacaaa
    80881 accaaagcac gttaccaatc aaaagaagat cttgaaaaag ctaaaaaaga gcacggtatt
    80941 acctatggcg agtgggtcaa tgataaggtt gcttattacc acgactatag caaagatggt
    81001 aaaaccgctg tcgatcaaga gcacggcaca cacgtgtcag ggatcttgtc agggaatgct
    81061 ccatctgaaa cgaaagaacc ttaccgccta gaaggtgcga tgcctgaggc tcaattgctt
    81121 ttgatgcgtg tcgaaattgt aaatggacta gcagactatg ctcgtaacta cgctcaagct
    81181 atcagagatg ctgtcaactt gggagctaag gtgattaata tgagctttgg taatgctgca
    81241 ctagcttacg ccaaccttcc agacgaaacc aaaaaagcct ttgactatgc caaatcaaaa
    81301 ggtgttagca ttgttacctc agctggtaat gatagtagtt ttgggggcaa gacccgtcta
    81361 cctctagcag atcatcctga ttatggggtg gttgggacac ctgcagcggc agactcaaca
    81421 ttgacagttg cttcttacag cccagataaa cagctcactg aaactgctac ggtcaaaaca
    81481 gccgataagc aagataaaga aatgcctgtt ctttcaacaa accgttttga gccaaacaag
    81541 gcttacgact atgcttatgc taatcgtggg atgaaagaag atgattttaa ggatgtcaaa
    81601 ggcaaaattg cccttattga acgtggcgat attgatttca aagataagat tgcaaacgct
    81661 aaaaaagctg gtgctgtagg ggtcttgatc tatgacaatc aggacaaggg cttcccgatt
    81721 gaattgccaa atgttgacca gatgcctgcg gcctttatca gtcgaaaaga cggtctctta
    81781 ttaaaagaca atcctaaaaa aaccatcacc ttcaatgcga cacctaaggt attgccaaca
    81841 gcaagtggca ccaaactaag ccgcttctca agctggggtc tgacagctga cggcaatatt
    81901 aagccagata ttgcagcacc cggccaagat attttgtcat cagtggctaa caacaagtat
    81961 gccaaacttt ctggaactag tatgtctgcg ccattggtag cgggtatcat gggactattg
    82021 caaaagcaat atgagacaca gtatcctgat atgacaccat cagagcgtct tgatttagct
    82081 aaaaaagtat tgatgagctc agcaactgcc ttatatgatg aagatgaaaa agcttatttt
    82141 tctcctcgcc aacaaggagc aggagcagtc gatgctaaaa aagcttcagc agcaacgatg
    82201 tatgtgacag ataaggacaa tacctcaagc aaggttcacc tgaacaatgt ttctgataca
    82261 tttgaagtaa cagtaacagt tcacaacaaa tctgataaac ctcaagagtt gtattaccaa
    82321 gcaactgttc aaacagataa agtagatgga aaacactttg ccttggctcc taaagcattg
    82381 tatgagacat catggcaaaa aatcacaatt ccagccaata gcagcaaaca agtcaccgtt
    82441 ccaatcgatg ctagtcgatt tagcaaggac ttgcttgccc aaatgaaaaa tggctatttc
    82501 ttagaaggtt ttgttcgttt caaacaagat cctacaaaag aagagcttat gagcattcca
    82561 tatattggtt tccgaggtga ttttggcaat ctgtcagcct tagaaaaacc aatctatgat
    82621 agcaaagatg gtagcagcta ctatcatgaa gcaaatagtg atgccaaaga ccaattagat
    82681 ggtgatggat tacagtttta cgctctgaaa aacaacttta cagcacttac cacagagtct
    82741 aacccatgga cgattattaa agctgtcaaa gaaggggttg aaaacataga ggatatcgaa
    82801 tcttcagaga tcacagaaac catttttgca ggtacttttg caaaacaaga cgatgatagc
    82861 cactactata tccaccgtca cgctaatggc aaaccatatg ctgcgatctc tccaaatggg
    82921 gacggtaaca gagattatgt ccaattccaa ggtactttct tgcgtaatgc taaaaacctt
    82981 gtggctgaag tcttggacaa agaaggaaat gttgtttgga caagtgaggt aaccgagcag
    83041 gttgttaaaa actacaacaa tgacttggca agcacacttg gttcaacccg ttttgaaaaa
    83101 acgcgttggg acggtaaaga taaagacggc aaagttgttg ctaacggaac ctacacctat
    83161 cgtgtccgct acactccgat tagctcaggt gcaaaagaac aacacactga ttttgatgtg
    83221 attgtagaca atacgacacc tgaagtcgca acatcggcaa cattctcaac agaagatcgt
    83281 cgtttgacac ttgcatctaa accaaaaacc agccaaccgg tttaccgtga gcgtattgct
    83341 tacacttata tggatgagga tctgccaaca acagagtata tttctccaaa tgaagatggt
    83401 acctttactc ttcctgaaga ggctgaaaca atggaaggcg ctactgttcc attgaaaatg
    83461 tcagacttta cttatgttgt tgaagatatg gctggtaaca tcacttatac accagtgact
    83521 aagctattgg agggccactc taataagcca gaacaagacg gttcagatca agcaccagac
    83581 aaaaaaccag aaactaaacc agaacaagac ggttcaggtc aagcaccaga caaaaaacca
    83641 gaagctaaac cagaacaaga cggttcaggt caaacaccag ataaaaaacc agaagctaaa
    83701 ccagaacaag acggttcagg tcaaacacca gataaaaaac cagaaactaa accagaaaaa
    83761 gatagttcag gtcaaacacc aggtaaaact cctcaaaaag gtcaaccttc tcgtactcta
    83821 gagaaacgat cttctaagcg tgctttagct acaaaagcat caacaagaga tcagttacca
    83881 acgactaatg acaaggatac aaatcgttta catctcctta agttagttat gaccactttc
    83941 ttcttgggat tagtagctca tatctttaaa acaaaacgca ctgaagatta gtacaactga
    84001 tcagatggct actattgatg atgagttaga tcagtcttct tttcaaaaga aggcatagca
    84061 gtataaaaat agatatgtga ggacaatatg cttagagcag aaaataacaa acacagccgc
    84121 tattctattc gcaagctgag cgttggggta acgagtatag caattgcgag tctcttttta
    84181 ggaaaggttg cctatgccgt agatagcatg cagcaaaact cttcgactca aagaaacaca
    84241 gccaatacat ccaaaaaatg gtatcatatt gatgataggg gctttattcc tttaggtata
    84301 agcttagaag ctgccaaaaa agattttaaa gaagaagtag aaaaatcacg tttgtctgaa
    84361 gaacaaaaac aaacgtataa tcaaaaaatt gatgctgaaa cagacaaaga cgaactattg
    84421 tcaacgtatc atagagacta tctgacagcc gttaagaatc ttccaacgtc tactgagcca
    84481 gttgaggcac ccgtgcagga gacacaggca taagtttcag attcgatggt gacaggtgat
    84541 tcaacatcag ttacgactga ttctcctgag gaaacactat ctacagaaac cccatctcca
    84601 tcacctgaga gtgagaaatc ttcagtagca gcttcttctg aggaaacctc atctccagaa
    84661 actccatcaa ctccagaggc cccagtagcc ccagctttat ctgaggctcc atctccatca
    84721 cctgagagtg aggaaccttc agtagcagct tcttctgagg aaaccccatc tccatcaact
    84781 ccagcagccc cagaaactcc agcggctcca gaaactccag cggctccaga aactcctgag
    84841 gaaccttcag tagcagctcc ttctgaggaa accccatctc catcaactcc agcagcccca
    84901 gaaatcccag ctgaatcaga ggctccagct caaccagctg agagtgaaga atcttcagta
    84961 gcagctacga caagtccgtc tccatcaact ccagctgaat cagagactca gacgccatca
    85021 gctgttacta aagactctga taagccatct tcagcagctg aaaaaccagc agcctcttca
    85081 cttgtttcag aacaaaccgt tcaacaacca acttcaaaga gatcttctga taaaaaagaa
    85141 gagcaagaac agtcttactc tccaaatcgc tcattgtcaa gacaggttat ggcccatgag
    85201 tcaggtaagt acttgccttc aacaggtgaa aaagcacagc cactctttat agctactatg
    85261 actttgatgt ctctatttgg cagtctttta gtcacaaaac gccaaaaaga aactaaaaaa
    85321 tagtattact taaactgata agagcttatt taatagagag aaccaatttg gttctctcta
    85381 tttttttaga tgtgcagcct catcaaaaag aagttaggac aaatagctgt caacaattgc
    85441 cttttgctta tcccttcagt tgctgtgatt ttatcataca gtgataaaag atggtagttg
    85501 ccattgacaa agcattgata aaggagtaaa attaactggt taataactgg ttaaattata
    85561 attgaggagg tactatgaaa aaaggttttt ttctcatggc tatggctgtg agtttagtaa
    85621 tgatagcagg gtgtgataag tcagcaaacc ccaaacagcc tacgcaaggc atgtcagttg
    85681 taaccagctt ttacccaatg tatgcgatga caaaagaagt atctggagac ctcaatgatg
    85741 tgaggatgat ccaatcaggt gcaggcattc attcctttga accgtctgta aatgatgtgg
    85801 cagctattta tgacgcggat ttgtttgttt accattcaca taccttagaa gcttgggcaa
    85861 gggatctaga ccctaattta aaaaaatcaa aggttgatgt gtttgaagcg tcaaaacctc
    85921 tgacactaga tagagtcaaa gggctagaag atatggaagt cacacaaggc attgaccctg
    85981 cgacacttta tgacccacat acctggacag atcccgtttt agctggtgag gaagctgtca
    86041 atatcgctaa agagctagga cgtttggatc ctaaacacaa agacagttac actaaaaagg
    86101 ctaaggcttt caaaaaagaa gcagagcaac taactgaaga atacactcaa aaatttaaaa
    86161 aggtgcgctc aaaaacattt gtgacgcaac acacggcatt ttcttatctg gctaaacgat
    86221 tcggcttgaa acaacttggt atctcgggta tttctccaga gcaagagccc tctcctcgcc
    86281 aattgaaaga aattcaagac tttgtcaaag aatacaacgt caagactatt tttgcagaag
    86341 acaatgtcaa tcccaaaatt gctcatgcta ttgcgaaatc aacaggagct aaagtaaaga
    86401 cattaagtcc acttgaagct gctccaagcg gaaacaagac atatctagaa aatcttagag
    86461 caaatttgga agtgctctat caacagttga agtaaaggag attattagtg aagaaaacat
    86521 atggttatat cggctcagtt gctgccattt tactagctac tcatattgga agttaccaac
    86581 ttggtaagca tcatatgggt ccagcaacaa aggacaatca gattgcctat attgatgata
    86641 gcaaaggtaa ggcaaaagcc cctaaaacaa acaaaacgat ggatcaaatc agtgctgaag
    86701 aaggcatctc tgctgaacag atcgtagtca aaattactga ccaaggttat gtgacctcac
    86761 acggtgacca ttatcatttt tacaatggga aagttcctta tgatgcgatt attagtgaag
    86821 agttgttgat gacggatcct aattaccgtt ttaaacaatc agacgttatc aatgaaatct
    86881 tagacggtta cgttattaaa gtcaatggca actattatgt ttacctcaag ccaggtagca
    86941 agcgcaaaaa cattcgaacc aaacaacaaa ttgctgagca agtagccaaa ggaactaaag
    87001 aagctaaaga aaaaggttta gctcaagtgg cccatctcag taaagaagaa gttgcggcag
    87061 tcaatgaagc aaaaagacaa ggacgctata ctacagacga tggctatatt tttagtccga
    87121 cagatatcat tgatgattta ggagatgctt atttagtacc tcatggtaat cactatcatt
    87181 atattcctaa aaaagatttg tctccaagtg agctagctgc tgcacaagcc tactggagtc
    87241 aaaaacaagg acgaggtgct agaccgtctg attaccgccc gacaccagcc ccaggtcgta
    87301 ggaaagcccc aattcctgat gtgacgccta accctggaca aggtcatcag ccagataacg
    87361 gtggctatca tccagcgcct cctaggccaa atgatgcgtc acaaaacaaa caccaaagag
    87421 atgagtttaa aggaaaaacc tttaaggaac ttttagatca actacaccgt cttgatttga
    87481 aataccgtca tgtggaagaa gatgggttga tttttgaacc gactcaagtg atcaaatcaa
    87541 acgcttttgg gtatgtggtg cctcatggag atcattatca tattatccca agaagtcagt
    87601 tatcacctct tgaaatggaa ttagcagatc gatacttagc cggccaaact gaggacaatg
    87661 actcaggttc agatcactca aaaccatcag ataaagaagt gacacatacc tttcttggtc
    87721 atcgcatcaa agcttacgga aaaggcttag atggtaaacc atatgatacg agtgatgctt
    87781 atgtttttag taaagaatcc attcattcag tagataaatc aggagttaca gctaaacacg
    87841 gagatcattt ccactatata ggatttggag aacttgaaca atatgagttg gatgaggtcg
    87901 ctaactgggt gaaagcaaaa ggccaagctg atgagcttgc tgctgctttg gatcaggaac
    87961 aaggcaaaga aaaaccactc tttgacacta aaaaagtgag tcgcaaagta acaaaagatg
    88021 gtaaagtggg ctatatgatg tcaaaagatg gcaaggacta tttctatgct cgtgatcaac
    88081 ttgatttgac tcagattgcc tttgccgaac aagaactaat gcttaaagat aagaagcatt
    88141 accgttatga cattgttgac acaggtattg agccacgact tgctgtagat gtgtcaagtc
    88201 tgccgatgca tgctggtaat gctacttacg atactggaag ttcgtttgtt atccctcata
    88261 ttgatcatat ccatgtcgtt ccgtattcat ggttgacgcg tgatcagatt gcaacaatca
    88321 agtatgtgat gcaacacccc gaagttcgtc cggatgtatg gtctaagcca gggcatgaag
    88381 agtcaggttc ggtcattcca aatgttacgc ctcttgataa acgtgctggt atgccaaatt
    88441 ggcaaattat ccattctgct gaagaagttc aaaaagccct agcagaaggt cgttttgcaa
    88501 caccagacgg ctatattttc gatccacgag atgttttggc caaagaaact tttgtatgga
    88561 aagatggctc ctttagcatc ccaagagcag atggcagttc attgagaacc attaataaat
    88621 ctgatctatc ccaagctgag tggcaacaag ctcaagagtt attggcaaag aaaaacgctg
    88681 gtgatgctac tgatacggat aaacccaaag aaaagcaaca ggcagataag agcaatgaaa
    88741 accaacagcc aagtgaagcc agtaaagaag aagaaaaaga atcagatgac tttatagaca
    88801 gtttaccaga ctatggtcta gatagagcaa ccctagaaga tcatatcaat caattagcac
    88861 aaaaagctaa tatcgatcct aagtatctca ttttccaacc agaaggtgtc caattttata
    88921 ataaaaatgg tgaattggta acttatgata tcaagacact tcaacaaata aacccttaac
    88981 caaaagaaga tctcattatt aaagcactgc tttatcaaag caagttacgg tgattttgaa
    89041 gtcattttat gtaacgagta gtgataaaag ttggataata gcggttttct tttgcaaaga
    89101 aatggtatcc atgttagaat aataaaaaaa agaggaggat tctaatcatg tcagaagaaa
    89161 aattaaaatc aaaaattgag caagcatctg gtggcctaaa agaaggtgct ggtaagttga
    89221 caggtgataa agagctagaa gcaaaaggat ttgttgaaaa aacaattgct aaaggcaaag
    89281 aacttgcaga cgatgcaaaa gaagcggttg aaggtgctgt agatgccgtt aaggaaaaat
    89341 taaaataatt aaaaaaccag ctgtaagctg gttttttaat ggtgtgattt tgttagaaaa
    89401 agactcaata ggtaataacc atagatgtta ggtatgcttg aacagttgag tacgattctc
    89461 ttcaatcata tagcacgcgt cacaaagttt ttctaacata tcaaaatcat gactaataat
    89521 gatgagacct aaatttctat cctttacaat ctccaacagg cttttccata cactagcttg
    89581 tgtaatgcta tccaacatag tagtcatttc atctgcaata aggtatttcg tctctggatg
    89641 taatgaacgc acaatcgaaa agcgttgcaa ttcccctccc gagagttcac tagggcgacg
    89701 ctttagccat ttttcttgaa tgccaaaagc atctcgcaaa tcttgacttg gatagtaggc
    89761 ttcttccaaa ctttttttca taggccataa aggattcatg gtttgttcag gatgttgttg
    89821 gataagttgc acaggtctaa atgctttgtt aggcaaatga ctgccatcaa ctaatacttc
    89881 accagattta ggctgcaaaa aaccggcaag cactctggat aaactcgttt ttccacaccc
    89941 actttgtcca aatatcccta agatttgacc aggtgctacc tctaaatcaa tctccttaaa
    90001 aagccattga tcttttttat gataaaagcc aagcttttta gcctctaagg tcatgagtaa
    90061 ctcctttcaa aaagtcttgc tgagggagtg tgcgccataa acgtctcgca aattctgttt
    90121 gtaattgctc cccacctccg ctaaaaaagc tagctggggc tgtttcaata gcttttccct
    90181 ctttaaaaat agttatacga tccgcaattt gactagctgc tacaatatca tgagtgataa
    90241 agatgacgct tattccttta tctgcaaaag agcgtagttg atctaaaacc atttgcagag
    90301 catctggatg taatccaggg gtgggctcat ccgcaataat caaagaaacc gtatcactaa
    90361 tacacgttgt aaacaaaaca cgtcggagca ttccgccaga aagttggaaa ggatacaagt
    90421 caccatcact ttcttttaaa ccaaactgtt gaaataatcc ttcttgagta gccttagcat
    90481 tttctgagat acccaagcgc acctgatgct tgactttcat agatggatct aaataattaa
    90541 cggattgtgg aatcaacgtc atttcttttc ctcgcaactg tttaatgcgt ttagaagtta
    90601 gtgattgacc acgataaatc atatctcctg ttacagctgc atttttagga agaatatcca
    90661 taatagcgtg tgctaataaa ctcttccctg aaccactagc acctataata gctaacaact
    90721 cacctttttt aacttctaaa ttcagcgctt gtatcggtgt ggattgaaat ggttttaaaa
    90781 atcttccgta ttgagtgaag gtgatggaga ggtctttaat gcttaataat gtttctgtca
    90841 tatctctacc tcctaaaaat gatccgtttg agggtaaaag agtttcttta aagattctcc
    90901 gatagtatca aatgcattga caaccaaaat aagataaagg cctggaaaaa tcaccaacca
    90961 ccaattgcca agagagatat gcttagctgc ctctgataaa atgataccaa ccgaaggttg
    91021 ttcggcagaa aggccaaatc ctaagaaagt catggatgct tcatgcaaga tgacgtgcgg
    91081 aaataagagg ataaacccaa tgaaaatttg agaagcaatc aaaggcaaga tatgatgcct
    91141 cacaatataa taaggcgttt ttcccatgct tttagagagc tggacaaagg ctttattctt
    91201 tagatcatag acttcattgc ggataagcct tgctagagag ggccaatggg taacagctgt
    91261 tgcaatgata accccttgag cccctttccc aacaacaaaa gaaatgagaa tcataaaaat
    91321 caaatgaggc ataccaataa acaaatcaac caaccaggct attattttat caataaggct
    91381 atttcctaga cctgcaagca ctccaaaaac ggtcgcaaga aagactccca taagggcacc
    91441 taataagccg acttgtaaag agaaataaag ccctttaatc gttctgacaa acatatccct
    91501 acctaaacca tctgtcccaa aaagatggtt taaggaagga gcaaggttgc gtaaagctgc
    91561 attggtttcc aaaggcgtcc tatagaaata aaggtttaag gctagaatac taagaatgag
    91621 agaaatggcg atacccagtt gccataaaac catcgttcga cgtttcaata tcatactttt
    91681 ctccttaact gtggattgat gatgctattt aaaatgtccg caataaaatt gcccgcaaaa
    91741 acaaataatg tccctatcat cacaatagct agaagtagcg gtgtatcact tttaaggcct
    91801 gcttcagtga gggtagaccc taagccagga tatgagaaaa cttgctcagc aagaacagat
    91861 cctccaaaca actctccaaa ataggaaaaa tgcagtgtaa tagctggtac gatagcattt
    91921 ctaagacaat gatgtttaaa aatttgccac tgtgtttccc cacgcgctct ggcaaataag
    91981 acatattcac tagaaagcac cgacatcatt ttagttctcg tatgaagagt gacattggcg
    92041 atgcctagaa tacttagcgt gaaaacaggt aacataaggt gcttaatccg atcagctaac
    92101 gtaatatctt gactcaaagt gcctatcgga gaagaaatac caatcgggaa ccaccccagc
    92161 tggacagaaa agattaataa aaaaatgagt ccaatccaaa acgttggtac tgatatctga
    92221 aggtatgaaa accacctgat aactcggtca agtaatttcc cttgatggaa agcggataac
    92281 gttcctaaga taaatccaat aagacccgat aagatccaag agagtcccat aagtatgaaa
    92341 gaagcacctg cccgtgatct aataatatca ctaacaggtt gccgataaac gagcgaggtc
    92401 cctaaatgtc cctgtatcac atttttcaac caaataaaat actggactag agctggctta
    92461 tccaagccat agtggtgagc aatcgctttg tactgagcag gggttagtga tgtgtcataa
    92521 ttgacacttg ccatgactgg atctactgga gattgtttta acaaaacgaa ggtcaaaaca
    92581 gataccccaa aaataagcgt gacacatctg atgattttcc agatgataat aatggtagta
    92641 cgtttcacaa tgaaatctcc ttaatcgata agaaaaggag ccgaggaaac gatcagcaca
    92701 gttttgtgta tcttgttttc tcagactcca aacttaaagt cgctgtatta actaaacgcg
    92761 ttacttagtt gattcatccc aagtccactc ggcaatgtta gtcaataatg accaatcatg
    92821 accatgactg tggatgcctt gtttacctac attgatacgt ttatcaccaa tataagtatg
    92881 gttaaggctc accaaccata catttggcaa atctccaaga gtagaagcac ctgttttgcc
    92941 atcccactgc gctaacttcc aatattcgtt agctttgtca aggtcagaag atgtcattgc
    93001 tttgtcaagg tacttagtca cggtaggatt gttataaaac gtaatattgg tccaaccttt
    93061 acctgctagg cttggatgat gtgattcata aaattgctgc gcgtgatgac gtcctccggc
    93121 ataaagtaag gctgagtcat gtgacttcgt tgccatttca tcccagttac tagctttgag
    93181 tttaatagta atccctaggg ctttggcttg ctctgctact tcaacggcta agttcgctcg
    93241 caattgatca ttagtagggt agtacagatc aaacgctgca tcaaggtcac cttttttacg
    93301 gctaccgtct gcttgttctt tccatcccgc ttttgtcaac aattgcttag ctttagctac
    93361 tttattatct ttaatggctg tttttggatt ccaaaatggt gttttatcaa taattgaata
    93421 agctggttta ccataaccat ttaaaaccgt atcgagaact ttttgcctat ttaaaccaat
    93481 agtcaaggct ttgcggattg ctggatcact agtgacatca tttcctacag gataaccatc
    93541 aggagaatca gtgatgacgc cttttttcac ataaggtaat gataagccgc gcacatcatt
    93601 tgatggaata tcaaggaggc gggtgccttt gactttttta tcagcaagtt ctggtgtcgc
    93661 gtagatcata tcaacatcac cagattctaa agctgctagt gctgtgtttt catcaagtaa
    93721 gacccaagtc cactttttaa agtatggttt tttcccatgc cagtaagggt tacgaacaaa
    93781 aatagcttgt tctccagcct tatattcttt taccatgtaa ggtcctgaac cgataggatt
    93841 gctcttatac ttatcattgt aatgtttttt agggacgatt gggatttcag tcaactgtgc
    93901 tgtaaatgtc gaatgcgcct cagtcaaatg gatattgacc tgatttttcc caactacttc
    93961 aacgttcttg atgaaggtta gatcccaagc ctttccatct gctttcaaca tatcataagt
    94021 aaacttaaca tcatcagcag taacaggctc accatttgag aatttaaaat catcatgcaa
    94081 gtcaaacgac caagtcagcc catcttcaga gagatggtat gttttagcaa gctctccttt
    94141 tatatctagt tcaggagaac gtttcaatag agtgctatga gtgatattcc cttcattgtg
    94201 gactccataa cggtcctttg gatcgaattc atgagggagc tttgccccca tagaaacgac
    94261 aagttcgtct tttggacgtt gtttgcgctg acgttctttt gtttgaggct tttgttgttg
    94321 acatgcaact aaaataagcc cagtcaaaaa taacgtgata atagagaagt attttaggta
    94381 ttttg
//
LOCUS       DATURV010000002        81143 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DATURV010000002 DATURV010000000
VERSION     DATURV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 81143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81143
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.002"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            62..907
                     /locus_tag="VCF75_000076"
     CDS             62..907
                     /locus_tag="VCF75_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP2545678.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKTKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            1157..2221
                     /locus_tag="VCF75_000077"
     CDS             1157..2221
                     /locus_tag="VCF75_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEP2545679.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSANALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQKFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            2222..2914
                     /locus_tag="VCF75_000078"
     CDS             2222..2914
                     /locus_tag="VCF75_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2545680.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(2968..4338)
                     /gene="brnQ"
                     /locus_tag="VCF75_000079"
     CDS             complement(2968..4338)
                     /gene="brnQ"
                     /locus_tag="VCF75_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEP2545681.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEVTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
     gene            4572..5786
                     /gene="sstT"
                     /locus_tag="VCF75_000080"
     CDS             4572..5786
                     /gene="sstT"
                     /locus_tag="VCF75_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEP2545682.1"
                     /translation="MKKIYNLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGRQTNMTLIIFLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVVEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(5841..6515)
                     /locus_tag="VCF75_000081"
     CDS             complement(5841..6515)
                     /locus_tag="VCF75_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEP2545683.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(6525..7916)
                     /locus_tag="VCF75_000082"
     CDS             complement(6525..7916)
                     /locus_tag="VCF75_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEP2545684.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSIEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(7986..8699)
                     /gene="rsmG"
                     /locus_tag="VCF75_000083"
     CDS             complement(7986..8699)
                     /gene="rsmG"
                     /locus_tag="VCF75_000083"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEP2545685.1"
                     /translation="MTPQDFYRTLEKDGFLLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEKNEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            8849..9406
                     /locus_tag="VCF75_000084"
     CDS             8849..9406
                     /locus_tag="VCF75_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEP2545686.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            9453..10349
                     /gene="htpX"
                     /locus_tag="VCF75_000085"
     CDS             9453..10349
                     /gene="htpX"
                     /locus_tag="VCF75_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEP2545687.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            10583..11116
                     /locus_tag="VCF75_000086"
     CDS             10583..11116
                     /locus_tag="VCF75_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEP2545688.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPICLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            11383..12069
                     /gene="covR"
                     /locus_tag="VCF75_000087"
                     /gene_synonym="csrR"
     CDS             11383..12069
                     /gene="covR"
                     /locus_tag="VCF75_000087"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEP2545689.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            12075..13577
                     /gene="covS"
                     /locus_tag="VCF75_000088"
                     /gene_synonym="csrS"
     CDS             12075..13577
                     /gene="covS"
                     /locus_tag="VCF75_000088"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEP2545690.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            13792..14286
                     /gene="nrdR"
                     /locus_tag="VCF75_000089"
     CDS             13792..14286
                     /gene="nrdR"
                     /locus_tag="VCF75_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEP2545691.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVIQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            14270..15445
                     /locus_tag="VCF75_000090"
     CDS             14270..15445
                     /locus_tag="VCF75_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEP2545692.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAASIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGNLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            15446..16348
                     /gene="dnaI"
                     /locus_tag="VCF75_000091"
     CDS             15446..16348
                     /gene="dnaI"
                     /locus_tag="VCF75_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEP2545693.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            16411..17721
                     /gene="der"
                     /locus_tag="VCF75_000092"
     CDS             16411..17721
                     /gene="der"
                     /locus_tag="VCF75_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEP2545694.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVISAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            17928..21026
                     /locus_tag="VCF75_000093"
     CDS             17928..21026
                     /locus_tag="VCF75_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2545695.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASDTDKGDNENIFPNHARHL
                     RLPSGFFEEGVHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMFEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMQDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGESGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
     gene            21270..21872
                     /locus_tag="VCF75_000094"
     CDS             21270..21872
                     /locus_tag="VCF75_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545696.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            21912..23240
                     /locus_tag="VCF75_000095"
     CDS             21912..23240
                     /locus_tag="VCF75_000095"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEP2545697.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEKGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            23286..23768
                     /locus_tag="VCF75_000096"
     CDS             23286..23768
                     /locus_tag="VCF75_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2545698.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            23880..25454
                     /gene="mltG"
                     /locus_tag="VCF75_000097"
     CDS             23880..25454
                     /gene="mltG"
                     /locus_tag="VCF75_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEP2545699.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKDAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAFTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            25527..26009
                     /gene="greA"
                     /locus_tag="VCF75_000098"
     CDS             25527..26009
                     /gene="greA"
                     /locus_tag="VCF75_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEP2545700.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(26633..27556)
                     /gene="yidC"
                     /locus_tag="VCF75_000099"
     CDS             complement(26633..27556)
                     /gene="yidC"
                     /locus_tag="VCF75_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP2545701.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMTFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMSIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(27638..27916)
                     /locus_tag="VCF75_000100"
     CDS             complement(27638..27916)
                     /locus_tag="VCF75_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEP2545702.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGGIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGN"
     gene            28177..28914
                     /locus_tag="VCF75_000101"
     CDS             28177..28914
                     /locus_tag="VCF75_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEP2545703.1"
                     /translation="MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            28953..29453
                     /locus_tag="VCF75_000102"
     CDS             28953..29453
                     /locus_tag="VCF75_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP2545704.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            29468..30157
                     /locus_tag="VCF75_000103"
     CDS             29468..30157
                     /locus_tag="VCF75_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEP2545705.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            30335..30577
                     /locus_tag="VCF75_000104"
     CDS             30335..30577
                     /locus_tag="VCF75_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEP2545706.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            30755..31549
                     /gene="racE"
                     /locus_tag="VCF75_000105"
     CDS             30755..31549
                     /gene="racE"
                     /locus_tag="VCF75_000105"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEP2545707.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSVQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLAPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVEHRFFTTANSEIFQEIASIWLKQKINVEHVTL"
     gene            31546..32532
                     /locus_tag="VCF75_000106"
     CDS             31546..32532
                     /locus_tag="VCF75_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEP2545708.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQREIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDTILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            32511..33032
                     /locus_tag="VCF75_000107"
     CDS             32511..33032
                     /locus_tag="VCF75_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP2545709.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYSSLSKEFKR"
     gene            33029..33490
                     /locus_tag="VCF75_000108"
     CDS             33029..33490
                     /locus_tag="VCF75_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP2545710.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            33487..34233
                     /gene="xerD"
                     /locus_tag="VCF75_000109"
     CDS             33487..34233
                     /gene="xerD"
                     /locus_tag="VCF75_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEP2545711.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLVGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPMTLEKYYKS"
     gene            34233..34937
                     /locus_tag="VCF75_000110"
     CDS             34233..34937
                     /locus_tag="VCF75_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEP2545712.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKNNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNKVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            34934..35485
                     /locus_tag="VCF75_000111"
     CDS             34934..35485
                     /locus_tag="VCF75_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEP2545713.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            35606..36328
                     /locus_tag="VCF75_000112"
     CDS             35606..36328
                     /locus_tag="VCF75_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP2545714.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            36325..36588
                     /gene="yidD"
                     /locus_tag="VCF75_000113"
     CDS             36325..36588
                     /gene="yidD"
                     /locus_tag="VCF75_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEP2545715.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            36766..37314
                     /locus_tag="VCF75_000114"
     CDS             36766..37314
                     /locus_tag="VCF75_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEP2545716.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHIYEHDKLK"
     regulatory      37423..37547
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            37626..38189
                     /locus_tag="VCF75_000115"
     CDS             37626..38189
                     /locus_tag="VCF75_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2545717.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            38191..38844
                     /locus_tag="VCF75_000116"
     CDS             38191..38844
                     /locus_tag="VCF75_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP2545718.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILLIGLSRIYLGVHYPSDILAGFVLGFGILHFIYPFYKQKRFEWRFLLKQD"
     gene            39137..40057
                     /locus_tag="VCF75_000117"
     CDS             39137..40057
                     /locus_tag="VCF75_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEP2545719.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            40096..40650
                     /locus_tag="VCF75_000118"
     CDS             40096..40650
                     /locus_tag="VCF75_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP2545720.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            40783..42117
                     /locus_tag="VCF75_000119"
     CDS             40783..42117
                     /locus_tag="VCF75_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEP2545721.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQDQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            42195..42986
                     /gene="pflA"
                     /locus_tag="VCF75_000120"
     CDS             42195..42986
                     /gene="pflA"
                     /locus_tag="VCF75_000120"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEP2545722.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            43117..44052
                     /locus_tag="VCF75_000121"
     CDS             43117..44052
                     /locus_tag="VCF75_000121"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEP2545723.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            44128..44781
                     /locus_tag="VCF75_000122"
     CDS             44128..44781
                     /locus_tag="VCF75_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEP2545724.1"
                     /translation="MITIFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRSQLTLANYFYRLKRGEKPSAEQIDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(44826..45863)
                     /locus_tag="VCF75_000123"
     CDS             complement(44826..45863)
                     /locus_tag="VCF75_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP2545725.1"
                     /translation="MLFMVNKKRRPLLKSQRHLLRLYTSLILLLVSLMGLALSLGESH
                     LSFLDLVHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(45824..46876)
                     /locus_tag="VCF75_000124"
     CDS             complement(45824..46876)
                     /locus_tag="VCF75_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP2545726.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKSYHQLRLVLAGVMVSILLSALGQGITNYYHLTNAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYFLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(46866..47798)
                     /locus_tag="VCF75_000125"
     CDS             complement(46866..47798)
                     /locus_tag="VCF75_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP2545727.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(47824..48606)
                     /locus_tag="VCF75_000126"
     CDS             complement(47824..48606)
                     /locus_tag="VCF75_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2545728.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAIQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(48852..50297)
                     /locus_tag="VCF75_000127"
     CDS             complement(48852..50297)
                     /locus_tag="VCF75_000127"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEP2545729.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGLSFERLSYDSRQ
                     VDGKTLFFAKGATFKAEYLKEAIAKGLPLYVSEVDYKLDIPVVLVTDIKKAMSLIAMA
                     FYDNPQDQLKLLGFTGTKGKTTAAYFAYHILKESYKPALLSTMNTTLDGQTFFKSQLT
                     TPESLDLFAMMAECVKNGMTHLIMEVSSQAYLVGRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIAKHYL"
     gene            50385..52019
                     /locus_tag="VCF75_000128"
     CDS             50385..52019
                     /locus_tag="VCF75_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEP2545730.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGVVFSAIMYLGSPLFTSLSGGDAKLIPIMHSLSLAVLVFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFLIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLTAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWSFVNT
                     MSLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLLALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYAFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            52187..52816
                     /gene="upp"
                     /locus_tag="VCF75_000129"
     CDS             52187..52816
                     /gene="upp"
                     /locus_tag="VCF75_000129"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEP2545731.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEVHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            53040..53630
                     /locus_tag="VCF75_000130"
     CDS             53040..53630
                     /locus_tag="VCF75_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEP2545732.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            54125..54400
                     /locus_tag="VCF75_000131"
     CDS             54125..54400
                     /locus_tag="VCF75_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEP2545733.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKG"
     gene            54649..55284
                     /locus_tag="VCF75_000132"
     CDS             54649..55284
                     /locus_tag="VCF75_000132"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEP2545734.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLTKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            55302..56177
                     /locus_tag="VCF75_000133"
     CDS             55302..56177
                     /locus_tag="VCF75_000133"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEP2545735.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHTNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            56196..56436
                     /locus_tag="VCF75_000134"
                     /pseudo
     CDS             56196..56436
                     /locus_tag="VCF75_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tpl protein"
     gene            56840..57163
                     /gene="yabA"
                     /locus_tag="VCF75_000135"
     CDS             56840..57163
                     /gene="yabA"
                     /locus_tag="VCF75_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEP2545736.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            57168..58031
                     /gene="rsmI"
                     /locus_tag="VCF75_000136"
     CDS             57168..58031
                     /gene="rsmI"
                     /locus_tag="VCF75_000136"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEP2545737.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGAISEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            58058..58450
                     /locus_tag="VCF75_000137"
     CDS             58058..58450
                     /locus_tag="VCF75_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545738.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(58497..59126)
                     /locus_tag="VCF75_000138"
     CDS             complement(58497..59126)
                     /locus_tag="VCF75_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEP2545739.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTTENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDHIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQSLSQLTGTAIVHGTKII"
     gene            59426..59782
                     /locus_tag="VCF75_000139"
     CDS             59426..59782
                     /locus_tag="VCF75_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEP2545740.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGK
                     VLQVGYRKPYQELDLG"
     gene            complement(59856..60683)
                     /gene="xth"
                     /locus_tag="VCF75_000140"
     CDS             complement(59856..60683)
                     /gene="xth"
                     /locus_tag="VCF75_000140"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEP2545741.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDTQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYCLASNRIVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            60917..62098
                     /gene="lctO"
                     /locus_tag="VCF75_000141"
     CDS             60917..62098
                     /gene="lctO"
                     /locus_tag="VCF75_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEP2545742.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDYLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVNQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            62364..67307
                     /gene="scpC"
                     /locus_tag="VCF75_000142"
     CDS             62364..67307
                     /gene="scpC"
                     /locus_tag="VCF75_000142"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEP2545743.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLVGSVFLMMTTTVAADELTTTSEPT
                     ITNHAQQQAQPLTNTELSSAESQSPDTSQVTPETNREKEQSQDLVSKPTTTELADTDA
                     APMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFGDFD
                     EDWENFEFDADAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDETKY
                     ESHGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDVMGSAESLFIKAIED
                     AVALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDP
                     LATNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVD
                     FKDIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHG
                     ALGVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVS
                     KAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIA
                     GASLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNI
                     DGAVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKDKTLRYDTELLTDHVDPQ
                     KGRFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRF
                     RDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGK
                     HFTGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNN
                     QDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTL
                     LGTAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDP
                     ETNRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVADNKTFVERQA
                     DGSFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKE
                     TLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKG
                     LKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQ
                     YVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVRE
                     EVFYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVED
                     RAGNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYY
                     NNSGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQ
                     ITAHFDHLLPEGSRVSLKTAQGQLIPLGQSLYVPKAYGKTVQEGTYEVVVSLPKGYRI
                     EGNTKVNALPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSAT
                     AKALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            68091..68798
                     /locus_tag="VCF75_000143"
     CDS             68091..68798
                     /locus_tag="VCF75_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEP2545744.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSFIL
                     TGIAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALIPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            69038..71038
                     /gene="metG"
                     /locus_tag="VCF75_000144"
     CDS             69038..71038
                     /gene="metG"
                     /locus_tag="VCF75_000144"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEP2545745.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
                     EVEWVSEESYFLRLSKYADCLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADLSQLIDAQLADYHKYMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALAAFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVA
                     NLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            71532..72545
                     /gene="nrdF"
                     /locus_tag="VCF75_000145"
     CDS             71532..72545
                     /gene="nrdF"
                     /locus_tag="VCF75_000145"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP2545746.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            72549..73037
                     /gene="nrdI"
                     /locus_tag="VCF75_000146"
     CDS             72549..73037
                     /gene="nrdI"
                     /locus_tag="VCF75_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP2545747.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQTNNLITERTHPCHKPMRHT
                     SH"
     gene            73004..75184
                     /gene="nrdE"
                     /locus_tag="VCF75_000147"
     CDS             73004..75184
                     /gene="nrdE"
                     /locus_tag="VCF75_000147"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP2545748.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLVNPAIKK
                     TYISARKFFQFIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVEAGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDKATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTASGASDETTTECQSCMI"
     gene            complement(75390..76142)
                     /locus_tag="VCF75_000148"
     CDS             complement(75390..76142)
                     /locus_tag="VCF75_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEP2545749.1"
                     /translation="MLKKRYQLAIVLLLSCFSLIWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLIYPRAEQEAMAAYTCQQAGPINTSLDKAKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHTDITSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTVLKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDHKKLHIEACFKGSL"
     gene            76600..77046
                     /locus_tag="VCF75_000149"
     CDS             76600..77046
                     /locus_tag="VCF75_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545750.1"
                     /translation="MKTKSKRFLKLATLCLALLGTTLLMTRPVKAEVVMTQSLAEGSN
                     SSEKNSEQQDQDKVQQRGDSKEDWDRGYDDGYKKGEESDTREELDRSTFDSFPPGISD
                     QGEYLDGYEGGYEAGWRKGHPVEAFLEMAWHFLTYAFTSLFGNHGS"
     gene            77202..77558
                     /locus_tag="VCF75_000150"
     CDS             77202..77558
                     /locus_tag="VCF75_000150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545751.1"
                     /translation="MTYPVKATETPLSGYESTEQALPKSNQVTETLHSEGDLQKIAEK
                     SDDGYKLGYQKGYEAGRKEDASENPDENKLESKSLDYISGYYAGYYRGWDETNKPFQT
                     LLEDIYRWLWEIFFKP"
     gene            78081..78539
                     /locus_tag="VCF75_000151"
     CDS             78081..78539
                     /locus_tag="VCF75_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545752.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLTTQPVKAEEGQNKQQEVSVS
                     SQSGQSEGEDLSEDSDDKYPYAEGHEAGQDFGYKEGLKAGWQEADPKEPKDIPEPEFN
                     PYESEGEDRNDFKEGFRDGYPAGYVEGWRKSHPLWAIVQDVLPWLYNFLR"
     gene            complement(78621..80119)
                     /locus_tag="VCF75_000152"
                     /pseudo
     CDS             complement(78621..80119)
                     /locus_tag="VCF75_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612207.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(80316..81132)
                     /locus_tag="VCF75_000153"
                     /pseudo
     CDS             complement(80316..81132)
                     /locus_tag="VCF75_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT        25764 a        14858 c        16641 g        23880 t
ORIGIN      
        1 aaaactatat ctaattaaga tacaataatg atcgtaaaca aaatgaatcg aggataacgc
       61 tatgtctttt aaaaaatgga ttggaatagt cggaatcgct ttagcatcaa ctgtattggt
      121 tgcttgttct gaaaaacaag atgataaaaa cactctaact attggggtga tgacaaaaac
      181 agaatctgat caagcgagat gggataaagt tgaagaatta ttgaaaaaag acaatatcac
      241 tttgaagtac aaagaattta cagattactc gcaacctaat aaggcagttg ctaatggtga
      301 agtcgatatt aatgccttcc aacactataa tttcttaaat aactggaata aggaaaataa
      361 ggaacatttg gtggccattg ctgataccta tatcagccca attaaccttt tctctggaac
      421 cagtcaagat gggaagacta aatacaaatc agtagctgat ctgccaaatg gtgctcaaat
      481 tgcagtacca aatgatgcga ccaatgaaag ccgtgccctc tatgtgctgc aatcagctgg
      541 cctcattaaa ttgaatgtgt ctggtgatca attagcaacc attgccaata tctcagagaa
      601 caaaaagaaa ttagatatca aggaattaga tgctagccaa acggcacgtg ctttagtatc
      661 tgctgatgca gctgttgtca ataatagtta cgctgttcct gcaaaaattg actacaaaac
      721 ctctcttttc aaagaaaaag cagatgacaa ttcaaaacag tggattaaca ttattgcagg
      781 tcaaaaagat tgggaaaaat cagagaaggc tgatgctatt aaaaaactca tcaaggccta
      841 ccaaactgat gaggtgaaga aagtcgtgga aaaaacttca aatggtatag atgtatccgt
      901 atggtaaatt ttagccattg ttataacaag agtgtggtcg tgtttaaaac acatgctttc
      961 ttgcatgctt gaggaagagt ttacgactaa tttaccttta gaaagctatt taagaccttt
     1021 tcccttttag gatttagaaa ccttgggaag actattgcat gttatcttaa tagtcatgat
     1081 tatgcaggcg atgaaaacat tgctatcgta gattactgtc gccatatcgt actaatagag
     1141 gagttattaa aatcttatga atgaagcaat aattcaatta gatcatattg atattacctt
     1201 ccgtcaaaaa aaacgggtta tcgaagctgt taaagatgtc acggttcata tcaatcaagg
     1261 agatatttat ggcattgttg ggtattcagg tgccgggaaa tcaactcttg tgcgtgtgat
     1321 taacctgcta caggcaccaa caaacgggaa aatcactgtt gatggggatg tgacctttga
     1381 ccaggggaaa attcaattgt cagccaatgc ccttcgtcaa aaacgacgtg atattggcat
     1441 gatcttccaa catttcaatt tgatggccca aaaaacagcc aaagaaaatg tagcatttgc
     1501 gctccgccat tcatcgttaa gtaagactga gaaagaacat aaagttatcg agctgctaga
     1561 acttgtaggt ttatcagagc gagcagataa ctaccctgcc cagttatcgg gtgggcaaaa
     1621 acaaagggtg gctattgcgc gtgctctagc taacgatcca aaaattttga tttcagatga
     1681 ggcaacatca gcactagatc caaaaaccac taaacaaatt ttggcgctct tgcaagaatt
     1741 aaaccgcaaa cttggcctta ccattgttat gattactcat gaaatgcaaa ttgttaagga
     1801 tatttgtaat cgtgttgctg ttatgcaaaa tggtgttttg attgaagaag gttctgtcct
     1861 tgatatcttt tcaaatccaa aagaagcctt gacacagaaa tttatcacaa cagctacagg
     1921 gattgatgag gctttagaaa aaattaacca acaagacatt gtcaaacatt tgcctgcaaa
     1981 tgcccttcta gctcagttga aatatgcagg aacttctacg gatgagcctc tacttaatag
     2041 catttaccgt cagtttgaag tgacagctaa tattttgtat ggcaatattg agattttaga
     2101 tcatattcca gttggtgaca tgattgttgt cttggaaggg caagctgaaa atattcttgc
     2161 ggctgagaaa gccttgcatg aagctggtgt tgatgttagt attttaaaga gaggagccta
     2221 gatgtcacag ttaattcaaa cttatttgcc aaatgtttat gaactcggat ggtctggaga
     2281 tgctggttgg ggcttagcga tttggaatac tttatacatg accattgtgc cttttattgt
     2341 tgggggtgct attggtctct tattaggctt gttattagtt ttaacaggac cggacggtgt
     2401 tattgaaaac aaaacgattt gttgggtcat tgataaagtt acctctattt ttagagccat
     2461 tccttttgtt attttgattg ctattttagc tagctttacc tatttgcttt taagaacaac
     2521 tttaggggca acagcggcct tggtaccttt aacctttgcg acttttcctt tttatgcccg
     2581 tcaggtccaa gttgtttttt cagaattaga caagggtgtt attgaagcag cgcaagcttc
     2641 aggtgctact ttctgggata ttgtcaaagt gtatcttagt gaaggtttac cagatcttat
     2701 tcgtgtttct acagtcacct taatttcttt agttggggaa actgccatgg caggagctat
     2761 cggtgctgga ggattaggaa atgtagcgat ttcttatggt tataatcgtt ttaacaatga
     2821 tgtgacttgg gtagcgacta ttattattct tttaattatt tttgctatcc aatttattgg
     2881 tgatagcttg actagaaggt ttagtcataa ataaacgaaa caacacccta agtttttact
     2941 tagggtgttg ttgtttagat gatgtcatta gcttttcttc gttttactta gtataagccc
     3001 tattaaaaaa ccaatagcag caaagataat ccatcccatt gagaattgcc ctagtggaac
     3061 agttttttgg aaaaaagtta caacggcttc tggtaaatga aataatccag tcatttgtga
     3121 tagtgttagg agtgcatcaa atatggcagg aataaaggtt agaccaattg tagttcgata
     3181 gacaatagga tcattattga agcatttttg tgccaaaaca aggaaaatta aatcaactgt
     3241 taatgggtat aaaaggaata aaacaggagc tgaccagttg ataataactg ataaaccacc
     3301 aaaataaaag aaagctgata gtaaagtaaa gatagttgcc caagcaatat gagataaagc
     3361 aggcactaat ttatggaagt attcagcact tgacgtgatt aagcctgttg aagtggttag
     3421 acaggccagg aaaatcacaa cagctagaaa tgcttgtccg atgccaccta ggtaaaaacg
     3481 agaagcatga cttaagattt gaccgccatt aactggatta ccatgaaggg taaagctgcc
     3541 atcactaaaa ggaaataatg attgtgatgt tgctccaata cgaccgacaa agatatagac
     3601 aagtgctaat agcaaaatgg caatagcccc agaaataagt gttattttgg tcacttcttt
     3661 gtccgtctta gcgccaaatt gtttggtagc ctcaatgact aaaatagcaa aaacaagaga
     3721 agctagtgca tccatagtgc cataaccttg aattaatcct gctataaaag gaaaatcttt
     3781 aaaggcatta ttaacaccga ccccagcgtt aaaagcatca ccgtaatttc cagcaggatg
     3841 gacaaaggac gcgataacca aaatagaaat aacaactaac aacgttggcg ttaaaaattt
     3901 tccgatattt tcagctagct tactaggttt gatagcaagg aagtaggaca gaccaaaaaa
     3961 tagtatagca taaatagctt tattggttgt attatctcct aagataggag cgataccgac
     4021 tgaaaaagaa gtggctcctg ttcttggaat agcaaaaaat gggccaatag ataaatacaa
     4081 aatggaagaa tagaagattg cataccattt agagatgggg cgtgccaaac tttcaacatc
     4141 gcctgatcca gatttagcaa cagcaatgac accgagtaaa ggcaaggaga caccagttaa
     4201 acaaaaacca atgatagacc atagaatctg atgacctgag tagatgccta aaaaagctgg
     4261 gtaaatgagg tttgctgctc caaaaaatag cgcaaatagc atgaatccaa tgacgatata
     4321 tacatttttt tgtttcattt ataaatagtc tttctattga tactaatccg aagattctaa
     4381 caattttaat tatattatgt gtcaaaataa caacaaaggt tattatatct aagattactt
     4441 cgatagtcaa tagaaattga ctgaaaattc ttagcacaaa aaatggttaa gatatgtagc
     4501 tcattcgatt tgacgaatag atagaattat attattataa aaaacatgta actttagtaa
     4561 ggatgtaacg tatgaaaaaa atatataatt tatgggttag ggttagtcta atcaaaaaaa
     4621 taggtatcgg tgttgtaata ggagtcatgt tgggcatttt ggctcctgat cttacaggtt
     4681 ttagtatttt aggaaagtta tttgttggtg gtttaaaggc tatcgcacct ttgcttgtat
     4741 ttgctttggt ttcccaagcc atctcccatc agaaaaaagg gagacagact aatatgacat
     4801 taatcatttt tctgtattta tttggtacct ttgcgtcagc tttagtagca gtactgacag
     4861 cttatctatt ccctttgacg ttagtgttaa atacacctgt aaatacagaa ttatcaccac
     4921 ctcaaggtgt tgttgaagtt tttcagtcac ttctattaaa attagtggat aatccgatca
     4981 atgctttagc tactgccaat tatattggtg tgctatcatg ggcaattatt tttggactag
     5041 cattaaaggc tgcaagtcaa gaaacaaagc atcttataaa aacagcggca gaagttactt
     5101 cacaaatagt tgtttggatc atcaatctgg cacctattgg gattatgagt ctggtgttca
     5161 ctacgatttc tgaaaacggt gttggaatct tatctgatta tgcttttttg atactagttt
     5221 tagtaggaac aatggttttt gtcgccttag tggttaatcc acttattgcg gttttgatta
     5281 cacgacaaaa tccttatccc cttgtcttga gatgtttacg tgagtcaggt cttacagcat
     5341 ttttcacacg aagttctgca gccaatattc cagtcaatat gcaattatgc caaaaaattg
     5401 gattgagtaa agatacttat tcagtgtcaa ttccacttgg agcaactatt aatatgggtg
     5461 gggcagcaat taccatcaat gtcctaacgc ttgctgctgt acacactttt ggtattccta
     5521 ttgattttct aacagccctc ttacttagcg ttgttgctgc tgtctctgct tgtggtgctt
     5581 ctggtgttgc tgggggatcg ttgttactta ttcctgtagc atgtagtttg tttggcattt
     5641 caaatgatct tgcgatgcag gtggttggtg ttggttttat tgttggtgtt atccaggatt
     5701 cttgtgagac agccttaaat tcgtcaaccg atgttctttt tacagctatt gctgaaaatg
     5761 ctttttggaa acgtaaaaaa gcatagctag atttaacata gtaaaaaaca tccttggaaa
     5821 acgccaagga tgttttttta ttatttaaga tagccaagat aatcaaattt ttcaaaggtg
     5881 tggtcattag cgatggccac aatgatagta ttcggttcta aaggttcaaa aggtttgaca
     5941 ttagtatcta aggttttaac ttctttttga cgcatgccaa tcacatttaa ctcatattta
     6001 cgacgaacat ctaactctga aagagattgt ccttcccagc ttttaggaat gacaaattca
     6061 atcatcgaaa taccatgttc caaatagatg atgctttcaa tatgtcgtct gagaaggttt
     6121 gaagctacac gttttccaga atcacgttca ggggtgatga ctttagttgc tccgatgcca
     6181 tatagcactt cttcaaaaat tttattttta gccttggcaa taatggttgg aacaccgagt
     6241 tttttacagt gcatgactgc aagaacagaa gattccaagt tattccctga agcgataacg
     6301 acagtatcac aatgttcaat ccctacagct aatagaaatt ctttgtcagt aatatctcca
     6361 acagcggctt tagtgaccaa gtcggccacc tctttaacat gactttctct gatatcaata
     6421 gcaataacgt cttgatcaaa attacttaat tctctggcaa cagtacggcc aaaaatacca
     6481 aggccaagaa cgccgacagt tttacgtttt aacatgtttt ctccttaacc cactagaata
     6541 tcagtagtgg catattgaat ggttttttct tttcgttgaa tcagactgat gaggacagta
     6601 attgggccaa cgcggccgac gaacatcaag acaatgacaa taagacgtcc agcagtagac
     6661 aattgtggtg tcaaatccat agatacccca actgtcgcga tggcagaaat agattcaaat
     6721 aagagtggta taggagcgat atgaggctcc acgctaagta ataaaataaa acctatcata
     6781 agaactgcaa aaaagaagat aagaactgtc atggtctgct taattgtctt atttgcgata
     6841 attcggttac gaaaggtaac ttctgattgc cctgaaagtt ccgctttaaa gagtaagaaa
     6901 gtaatggcag cagtcgtcac tttaatacca ccagctgttc caccaggagc accaccaata
     6961 accatttgaa tcatataaag aatgttagtc ggtgctaggg tatcgttata agagattgtt
     7021 gcaaagccag ctgttctcat agtgactgtt tgaaaaaatg aaaccataag ttgctgatgt
     7081 aaactaaaat tggcaatggt tttgctgtta tctttttcta aaaaccaggt taagaaggtt
     7141 ccaagaaaaa gaataacagc tgtggtttgt agtactagac gagactgatt ggataattta
     7201 cgaaaagttg ccccataaca atgaggtctt tcaaaaaagt attttttaaa agctactcct
     7261 aaatcaaccc aaaccgcaaa accaagtcca ccagagataa tcaagaacgt aatgataaca
     7321 tttaatgtag gatttaacat aaagtctttt aaactagaag atcctaaatt gtcaaaacca
     7381 gcgttacaaa atgccgacac agctaaaaaa atactattga agataccatt tttccacccg
     7441 aagcggggga taaagtcaat catgataact atcgcagcaa aggcttctat actaaaggtt
     7501 actttataag caaaaaaaag gtagtgcttt aaatctttgc tatcacctcg atttaacgct
     7561 gattggagca aggtttgatc gctcaatcgc atttttcgct tgagggcaaa agtactcaca
     7621 gctattaacg tcaccaagcc aagaccacca atctgcatta gtgccatagc aatggtttgt
     7681 ccgataccgt tatacacttc agcgacggga acaacagaaa gtccagtcac acaaaccatg
     7741 gaaaccacat taaagaaatg atcaagatag acggtattag gaccattttg ataatgggta
     7801 aagggcatgg ataacaagag agtcccaatc agaatcacaa tggcaaaact aaatgttaag
     7861 cgctgtgtga ctgaaagaga tttgataaaa gagcgtttca tgtttcctcc agacataata
     7921 tctcctacta taataacaaa aataagttaa aaaagctaag tttcatctta gcctttggta
     7981 ctattttata gaggtttttt gttaggcaag ccagcttttc ttggatactt gttaggtgtt
     8041 tcctttttct tctcaacaat ggtgatgaag cgagaatctc catttggcaa ttggtagcta
     8101 tggtttttaa tgactttacc aaaaagaaga caaagagcat tcttagcttc ttctaactct
     8161 tggtcagcag cttgtgcctt taaggcgatt aattttcctc caatttttaa aaaggggatg
     8221 gttaattcag atagaacctg catccgagca accgctctgg cagtaacgac atcaaattga
     8281 cctcgaaagg ccttgtcctg tccgaaatct tctgctctac catgaaaaaa atggacattt
     8341 tccaagccca attcctgagc aaggagtgtc agaaaagaaa tccgcttatt aagagaatca
     8401 ataatggtaa cctcaaggtt tgggaaaaga attttcatgg gtaggctagg aaaaccagct
     8461 ccagctccaa tgtccaataa tttgattggt tcattagcta aaaagccctg taaaatagga
     8521 gcgatggaat cataaaaatg cttgaggtaa acttcatttt tttctgtaat agctgttaaa
     8581 ttaattttag tgttccattc cactagtaat ttgaagtagg tgtcaaattg ctctttttgc
     8641 ttcgatgata ataaaaaacc atctttctct agtgttcggt aaaagtcttg gggtgtcatt
     8701 agtgtcctct gtttttgata gttgttacca ttttatcata aaatagccta taacaaaatc
     8761 caataaatca tcagaaataa cacctcatta cttgctatta gcctagaaat tgctataatt
     8821 gagacacaaa gattaaagga gacccactat gccaacagtt ttgattatct tagtcgtttt
     8881 aggagtgctt gcgctatggt taatgattag ttacaatagc ctggttaaat ctcggatgca
     8941 cacaaaagaa gcttggagtc agattgatgt gcaattaaag cgtcgtaatg acttgattcc
     9001 aaatcttatt gaaaccgtaa agggatatgc tagctacgag caaaagacat ttgaaaaaat
     9061 cactgatttg cgtgcgcgtg ttgcgaatgc ctcaactcct caagaaacca tggcggcttc
     9121 taacgaattg agtaaacaag tgaccagttt gtttgccgtt gctgaaaatt acccagactt
     9181 aaaagctaac gaaaacttct tgaaattaca agaagagttg accaatacgg aaaataaaat
     9241 ctcatattct cgtcaactct ataattcaac aacgtctaat tacaaccttc aattagaatc
     9301 tttcccaagc aatatcgctg gtaaattatt tggttttaaa ccaagtgaat tcttacaaac
     9361 accagaagct gaaaaagaag ttccaaaagt tgaatttaac ttttaaatat gaaattatca
     9421 tctagtaggc aatcataaga gaaaggagaa ttatgcttta tcaacagatt tcacaaaata
     9481 agcaacgaac agttgtttta ttagttgttt tcttcgctct cttagcactt attggagcgt
     9541 cagcaggcta tctgctgtta gataattatg ccatgggctt ggttcttgct cttgtcattg
     9601 gggtgattta tgctaccagt atgatttttc aatccaccag tcttgtaatg agtatgaaca
     9661 atgctagaga agttacagaa aaagaggctc caggcttctt ccatattgtc gaggatatgg
     9721 ctatggtggc ccagattccg atgccgagag tttttattat tgaagatcct tctttaaatg
     9781 cttttgcgac aggatctagc cctcaaaatg ctgctgttgc agcaacgaca ggtttacttg
     9841 aagtcatgaa tcgtgaagaa cttgaaggtg ttatcggcca tgaaattagc cacatccgaa
     9901 attatgatat ccgtatttcg actattgctg tagctttggc gagtgctgtt accgttattt
     9961 ctagtatcgg tgggcgtatg ctatggtatg gtggcggttc tcgcaggcaa agagatgacg
    10021 gagatgatga tgttttaaga atcattacgt tacttttgtc gttattatct ttgcttttag
    10081 cgcccctagt agcaagtttg attcagttgg ctatttctcg tcaaagagaa tatctagctg
    10141 atgctagttc tgttgaacta acaagaaatc cccaaggtat gattaaggct cttgaaaaat
    10201 tgcagttatc tcagccaatg aagcatcctg ttgatgatgc tagtgcagcc ttgtatatta
    10261 atgagcctcg caaaaaaagg agcttcagtt cattattcag cacccatcct cctattgagg
    10321 agaggattga aaggttaaaa aacatgtgag cgtttggggt ttttgctcaa gcctcacttt
    10381 tttatgtctt tgggcagata atctaaaatg gcaggaatta tcaaaaatat ctgaacaaaa
    10441 agaaaaaaat gagtgaaaca atggttaaaa atagaatgat ttaaatctta aaattaaaaa
    10501 acgtcagaaa ataaatgcaa aactattgac aaatctttta gatagtagta aaaataaatg
    10561 agagttttat taggagaaga tgatgttagc tatttccgaa atcagaaaac acccagacgg
    10621 tctctcgttt gatcgtttat gtgatgtcaa aagtatgtta cttgagcgtg accaacagat
    10681 tattgacata aaggcagtta aagctgtcgg taatgttcgc tatgataagg ggctttatct
    10741 gttagattat caattgagtt acgaagtgat attaccgtct agccgttcta tggttccgat
    10801 ctgtttaagt gaagtccaac acattcaaga attatttata gaggcgacag atttagctga
    10861 taagaaagag ttggttgaag acaatctggt attagtttta gacaaagacg ctattaacct
    10921 cgaagaaagt attgtggata atatcctact tgctattccg ctacaggtct tgacagagga
    10981 agaaaagaaa tcaaaagagt tgccagcagg tcaaaattgg gcagttttga cagaagaaga
    11041 ctaccagtgt ctaaaagaag aaaagcaaaa agaaaataat ccttttgcta gcttgcaagg
    11101 gttgtttgat gaataaaact gaatattaaa gatgtctgta atttttattt gaaaaaacag
    11161 ctattctttg atatactctt tagagaatat ggttacttat ctaaagtaga aggtattttg
    11221 accatagagg gcaaagaagg taatgactat tagatgcttc atattttatt cgctagaaga
    11281 ctatttgacc attagaactg aaatagtcta ggatatgaga tgaatttcat agttttcttt
    11341 ctgggagaaa aagatagatt aagaggataa gggttggtat aaatgacaaa gaaaatttta
    11401 attattgaag atgaaaagaa tctggctaga ttcgtttctc ttgagctgca acatgagggt
    11461 tacgaagtca ttgttgaggt caatggtcgt gaagggttag aaactgcttt ggaaaaagag
    11521 tttgatttaa tcctgcttga cttaatgtta ccagagatgg atggttttga agtgacccgt
    11581 cgtttgcaaa ccgaaaaaac aacgtatatc atgatgatga ctgcgcgtga ttctattatg
    11641 gatgtggttg caggtttaga ccgcggtgca gacgactata ttgttaaacc gtttgccatt
    11701 gaagaactac ttgcccgtat tcgtgctatt ttccgccgtc aagatattga atctgagaag
    11761 aaagtgccta gtcaaggcat ttatcgagat ctagttttaa atccacaaaa ccgttcagtt
    11821 aatcgtggcg acgatgagat ttctctcact aaacgtgaat atgatttgct taatattttg
    11881 atgactaata tgaatcgtgt catgacacgt gaagaattat tgtcaaatgt ttggaaatat
    11941 gatgaagccg ttgagactaa tgttgtagat gtctatattc gttatctccg cggcaaaatt
    12001 gacattccag gcaaggaatc ttatatccaa acagtgcgtg gcatgggata cgttattcgt
    12061 gagaaataag tcatatggaa aatcagaaac aaaaacagaa gaaatataaa aactcgttac
    12121 caaaacgact atctaatatc ttttttgttc tttttttctg cattttctct gcctttacac
    12181 tgattgccta tagttcaaca aactatttct tattgaagaa agaaaagcag tcagtctttc
    12241 aagctgtaaa tattgttaga gttcgtcttt ctgaggtgga ctctaatttt acattagaga
    12301 acttagcaga agttttgtac aaaaacgata aaacacatct gagaattgat gacagaaagg
    12361 gcagtcgagt cattaggagt gagcgcgata tcacaaatac tctagatgcg aatcaagata
    12421 tttatgtcta taacattgat aaacagatga tttttaccac agataacgaa gaatcatctc
    12481 ctggcttgca tggtcctatt ggtcgggtgt atcacgacca tatcgaagat cagtatcgtg
    12541 ggttttccat gacacaaaag gtatattcta atcggactgg aaaatttgtg ggctatgttc
    12601 aagtctttca tgatttaggc aattattatg tcattagagc aagactgctg ttttggctac
    12661 tagttgttga gttatttggc acaagcttag cctatttaat catcttaatt actacgcggc
    12721 gctttttgaa gcctttacac aatttacatg aagtgatgcg taatatctct gaaaatccta
    12781 ataatttaaa cctgcgctca gatatttcgt caggagatga aatcgaagaa ttgtctgtta
    12841 tctttgacaa tatgttagac aaattggaga cacatactaa gttgcaatca cgttttatta
    12901 gtgatgtcag ccatgaatta cgaacaccgg ttgcgattat taaagggcat attggtctct
    12961 tacaacgttg gggtaaagat gacagtgata tacttgaaga aagcttgaca gcaacagcgc
    13021 atgaggctga ccgtatggca atcatgatta atgatatgtt agacatgatt cgcgtacaag
    13081 gctcatttga gggacatcaa aatgatatga cagttttgga agattctatt gaaactgttg
    13141 ttggtaattt tagagtttta agagaagatt ttatctttac atggcagtca gaaaatccaa
    13201 aaacgatagc ccgtatttat aaaaatcatt ttgagcaggc tttgatgatt ctgattgaca
    13261 atgctgtgaa gtattcccgt aaagaaaaga aaatcgcgat taacctttca gtgactggca
    13321 aacaagaagc tattgttaga gttcaagata aaggcgaagg aatttctaaa gaagatattg
    13381 aacatatctt tgaacgcttt tatagaacgg ataaatcacg taatcgaaca agtacccagg
    13441 ctggattagg gattggcttg tctattctca agcaaattgt agatgggtat catttacaga
    13501 tgaaggttga aagtgaatta aatgaaggtt cagtgtttat cttacatatt cctttggccc
    13561 agtctaaaga gagttagagt agcttaaggc attgttaatg tgacaggtat agtatgtaat
    13621 cattagataa tagtagcagt cttttgccac aactggttag aagctgatgc tattttttta
    13681 gcaatgatgg atgacatgaa agaaatactg tcaatcgcga tgccttttgt tatccttgag
    13741 aatatggtaa aatagtgtat tagattaatt tgatagaatt ggggtgtttg cgtgcgttgt
    13801 ccaaaatgta attaccataa atcgagtgtt gttgacagta gacaagctga agacggcaac
    13861 accattcgtc gtcgaagaga atgtgaacag tgtcatactc gctttactac ctttgagcgt
    13921 gttgaagaac tccctttatt agtcatcaaa aaagatggca cacgagagca attttctaga
    13981 gataaaatat taaatggggt tatccaaagt gcgcaaaaac gacctgtctc gagcacagat
    14041 attgaaaatg tcatttctcg tattgaacaa gaagttcgaa caacctacga aaatgaagtg
    14101 tcaagtactg ctattggtaa tttagtgatg gatgagttgg ccgaacttga tgaaatcaca
    14161 tatgtacgct ttgctagtgt ctacaagagc ttcaaggacg ttgatgaaat cgaagaactt
    14221 ttgcaacaaa ttacgaatcg cgtacgcgga aagaaaaaac gtttaaataa tgatgaaacc
    14281 aattgatact tttacatatc taaaacgcaa caaagtaact cttgattcgg tgactttaat
    14341 acagttgtac tttccaatta ttggaagtga cgcagcctct atctatcaat actttattca
    14401 cttttttgac gatggtttac aacgtcataa attcagcgat attttaaatc acctacagtt
    14461 tggtatgaaa cgttttgaag atgctcttgc catattgaca gcaatggaat tagtgagtgt
    14521 gtatcagcta tcagatacat atctcattac gttgcaccag ccgcttagcc gtgacttgtt
    14581 ttttcaacat ccagcttatt ctcgattatt agaacaaaag attggagagg tagctgtctc
    14641 tgagttgcag gtaactgtcc caagtcaagc aagaaatatt tctaagcgat tttcggatat
    14701 ttttggggtg caaggaaatt tgacgaacgt acctcaaaag ccacaaaaga attttgacct
    14761 ttcaagtttc cagcaattaa tggttcgtga tgggttacaa tttgaagata atcaaaaaga
    14821 tatcatcagc ctttacagta ttgctgaaca gtatgatatg acgtggtttg atacttatca
    14881 aatagctaag gcgacggctg ttaatgggaa aattcgacct gaacgcttat tggctaaaaa
    14941 aaatcaaagc atgacaaaac caagcaaaga aaatttttca caggctgagc aaattatttt
    15001 gcgagaagca aagcaagaca gtgctttagt ctttttggaa aaaattaaaa aggctagacg
    15061 tgcaacgatt actaaggatg aacgcatttt attgcaaaca ttggccaaaa tgaattttct
    15121 tgatgatgtt atcaatgtca tggtgcttta tacctttaat aagacaaaat ctgctaattt
    15181 acagaagagt tatgtcttaa aaatggccaa tgatttcgcc taccaaaagg tgtcaacggc
    15241 tgaagaagct atagtggttt tgcgagcctt cacggatcgt caaagtcgtc ggcaatcaaa
    15301 agtaaaaaca agtcaaagta atgtgcctaa gtggagcaat cctgattacc aagagacaac
    15361 aagtcaagaa gagcaggcga aactagatca gttcaaacag gctgctttga agaggttaga
    15421 aaacctagga aaagggggtg actagatgga aaagattgga gaaaccatgg cgaaattggg
    15481 tcaaaacact cgtgttaata gcgaccagtt gattcaaacc attttagcgg atcctgaagt
    15541 agcaagcttc atcagtcaac atcacttatc tcaagagcaa attaacctta gcttatctaa
    15601 gttcaatcaa tttttggtgg aacgccagaa gtatcaactc aaagatccct cttatattgc
    15661 taagggctat cagcctattc tagctatgaa tgagggctac gctgatgtgt cttatttgga
    15721 aaccaaagaa ttggtagaag ctcaaaaaca ggcagctatc tctgaacgga ttcaactggt
    15781 tagtttgcca aaatcctatc gccatattca tttatcagat attgatgtta ataatgcgag
    15841 ccgtatggaa gccttctctg ctattttaga ttttgtggaa caatacccat cagcagagca
    15901 aaaaggctta tatttgtacg gagatatggg aattggcaag tcttacttgt tggctgctat
    15961 ggcccatgaa ttgtctgaga aaaaaggtgt gtcaacgacc cttctacatt ttccgagctt
    16021 tgccattgat gtaaaaaatg ccatttcaaa cggctctgtt aaagaagaaa tcgatgcggt
    16081 caaaaatgta ccagtgctca ttttagatga tattggtgca gagcaagcca cttcttgggt
    16141 ccgtgatgag gtgttgcagg tgattttaca ataccgtatg ttagaggaat tgccaacctt
    16201 ctttacctct aattacagct ttgcggattt ggagcgaaaa tgggcaacca tcaaaggaag
    16261 tgacgaaact tggcaggcca aacgtgtcat ggaacgcgtg cgttatttgg ctagagagtt
    16321 tcatttagaa ggggctaacc gccgttaagt ttgagatagc aatcaagaag aatagctaca
    16381 cgtatgagtg agtgaaagaa aaggagaatc atggttttac ctaccgttgc cattgtgggc
    16441 cgtccaaatg tgggcaagtc caccttattt aatcgaatcg caggagagcg catttcaatt
    16501 gtagaagacg ttgaaggggt tactcgtgac cgtatttatg ctactgggga gtggcttaac
    16561 cgccaatttt cattgattga tacaggtggt attgatgacg ttgatgcccc gtttatggag
    16621 caaatcaagc accaggctca aattgccatg gaagaagctg atgttatcgt ttttgttgtt
    16681 tctggcaaag aaggggttac ggatgcagat gaatatgtgt ctaaaatcct ataccgaacc
    16741 aatactccag tgattttagc tgtcaacaaa gtggataatc ctgaaatgcg taatgacatt
    16801 tatgattttt attcacttgg tttaggagat ccctatccag tttcttctgt tcatggtatc
    16861 ggaacagggg atgtcttgga tgcgatcgtt gaaaacttgc cagtagaaga ggctgaagaa
    16921 aatgatgata ttattcgctt cagtttaatc ggtcgtccta atgttgggaa atctagcttg
    16981 attaacgcta ttttaggtga ggaccgtgtt atcgctagcc ctgtcgcagg gacaacacgt
    17041 gacgctattg acacgcattt tacggatgct gacggtcaag aatttaccat gattgacacg
    17101 gctggaatgc gcaaatctgg caagatttac gaaaatacag agaaatattc tgtcatgcgt
    17161 gccatgcgtg ccattgaccg ctccgatgtg gtcttgatgg ttatcagtgc tgaagaaggt
    17221 atccgtgaat acgacaagcg tatcgctggc tttgcacacg aagctggtaa aggaatgatt
    17281 atcgtggtta acaaatggga taccattgac aaggataatc ataccgttgc taagtgggaa
    17341 gccgatattc gtgaccaatt ccaatttttg acatacgctc cgattatttt tgtgtcagct
    17401 ttgacgaagc agcgcctcaa caaattgcct gacttgatta aacgtatcag tgagagtcag
    17461 aataaacgta tcccatctgc tgtcctcaat gatgttatca tggatgctat tgccattaat
    17521 ccaacaccaa cggataaggg taaacgtttg aaaattttct atgccactca agtgtcagta
    17581 aagccaccaa catttgtcgt ctttgttaac gaggaagaat tgatgcactt ctcttatcta
    17641 cgcttcttgg aaaaccagat tcgcgcagcc tttacctttg aagggacacc gattcatctg
    17701 attgctcgca aacggaaata agaaatgaac atcaactaaa atgctgacct aagaaataat
    17761 ctcatcattg ataaggactg tgttgtgtat gttacaggag acagtccttt ttataatacc
    17821 tttaagggtg tagacggaag attagtagca aacaaagaaa aaggattttg acaagaaata
    17881 tcagtcggtt tttgctataa tgaaaggata agcaaaaagg tggaaaaatg atggctagat
    17941 taattccagg acgagtaagg aatgaaggta ttaagcttta tgagcaaggt ttagtctcat
    18001 tccaagatga taataaaggc attcttcaaa tagaagtgga gacttatcag gttcaatatg
    18061 gagcagatga tgaggatatc acttgtcaat gcgacacatt tcatatgaaa cattattgca
    18121 agcatattgc agcagtcgag tattttttaa aaaatgatca aaaaggtaag ctttttttaa
    18181 aacagttaac aaatcaaaca aaaatcaaag aaacaacaaa gaaaatgaca tcttttggta
    18241 gcctcttttt agatagctta gccatgaatg aagatgattc tgttaaatac aggctgtctg
    18301 ctcttggtag tcgaagccct ttttcgtcag attactggtg gagtctgaaa atcaatcgtt
    18361 taccagatga tcgttcttat gtgattcgag atattaaagg ctttttgcag cttattaaaa
    18421 aagaaggctt ttatcagatt ggaaaaaatt attttgaaca actttcatgg ctgcagtttg
    18481 acccatccag tcaagacctt attgagtttt tatggcgctt agcatctgat actgacaaag
    18541 gggacaacga gaacattttt ccaaatcatg caaggcattt gcgcttacct agtggttttt
    18601 ttgaagaagg cgttcactat ttgacgtcac tttatgattt tacttttgag gggccttctc
    18661 aaacttatca tcatcttttt gtccgttctt tggaagcaga ggcaggcctt tatgagttta
    18721 aagtggaagt gcatcgcaaa tctattgaat tgcaaattgc tgaaaaaaat gtgcaatacc
    18781 tctttgataa tgactatctt ctttatcaag atacctttta tcacttgacg ttaaagcaac
    18841 gaaaaatggt tcaggctatt cgtagtctgc ccattgaggc cgacttggct aaacatattc
    18901 attttgattt ggatgatcat gcgaaattag ctgcaagctt atcagatttt aaacagatcg
    18961 gtctagtaga ggctcctaaa agctttgcta ttagagattt tgaggtgact tttcaatttg
    19021 atttgttaaa tcgagatgaa atcagttgtc agcttatgtt tgattatggc aattaccaag
    19081 ttagtgacaa agcaagttta gaagcacttc cttttgctag tcatttaaaa aaagaagaaa
    19141 aaatcaatcg ttctttactt gcttttggct tctcgccaca attttactca aaaaagcgac
    19201 taacaagtgc aaaagagctc tatacttttt ttgaagaaac agttccatgt tttgagcgct
    19261 taggtaatgt tgcgttgagt acagctatcc aagcattaca agtgaaagaa atgcccaaga
    19321 tcgctattag acgcaatcag ggattactag atatttcgtt tgatttttca actattattg
    19381 aaaatgatat tgaccaagct gttacagctc tttttcaaaa taatccttat tttgtgagtc
    19441 aaacaggtca attagtggtt tttgatgatg aaactcaaaa agttagcaaa agtttacaag
    19501 aattaagagc aagacagcta aaaaatggtc acttgcaact ggatggcatt agagctctac
    19561 aagtatccaa actttttgaa ggcatgacat ctgttcattt ttcaaaggaa ttagaagagt
    19621 tagcctatca tttgcagcat cctgaaactt tttcaataaa accgttacca gttaaggctc
    19681 aaatgcgaga ttatcaacgc aacggtgttc agtggttatc aatgctcaat cattatggct
    19741 ttggtggcat tttagcagat gatatgggac ttggaaaaac tctccagaca cttgcttttt
    19801 tagctagtca cctgaaatca gatagtaaag tgctcatttt atctccatct agtcttatct
    19861 ataattggtt tgatgaatgt caaaaattca cacctcagct agatgttgtg gtttcttacg
    19921 gacttaaaca aatcagagat caaatcatcg aagaaggtca tcaaataacc ataaccagct
    19981 attcctcctt tagacaggat tttgagacct atcaagcgtt tcactacgat tatcttatct
    20041 tagatgaagc tcaagtgata aaaaatgcac aaactaagat ttcacactgt ttacgtgcgt
    20101 ttaatacagc caattgtttt gcgctttcgg gcactcctat tgaaaataaa atgtttgaaa
    20161 tttggtccat tttccaaatt gttttgccag ggttgctacc gactaaaaaa gagtttttaa
    20221 aattaacagc tgagcaagta tcacgctata tcaaaccctt tgtgatgcgt cgtaaaaaag
    20281 aagatgtcct accagaatta cctgatttaa ttgaaactaa ttattcaaac gagatgacag
    20341 atgaacaaaa agctatttac ttagcgcagt taaggcagat gcaagatcaa attcgaaatt
    20401 ctagtgatgt ggatattagc cgtcaaaaaa ttgagatttt atctggtatc actcgattga
    20461 ggcaaatctg tgacactcca agtctcttta tggattacca aggcgaaagt ggtaagctag
    20521 atagtttgcg aatcttattg acacaaatta aagaaaatgg tcatcgggct ttgatttttt
    20581 ctcaatttag agggatgctg gacttggcca agcaagagat gactgctcta ggattgacct
    20641 cttatcaaat gacgggatct acccctgcca atgaacgtca agaaatgact agagccttta
    20701 ataacggttc caaggatgct tttttgattt ccctcaaggc aggtggagtt ggaatcaacc
    20761 ttacgggagc agacacagtg attttaatcg atttatggtg gaatcctgct gttgaaatgc
    20821 aggcaattag tcgcgcctac cgtattggtc aaaaagaaaa cgttgaagta tatcgcttaa
    20881 tcactcgagg aacgattgaa gaaaagattt tagagcttca agaaagtaag cgcaatttgg
    20941 tgactacagt tttggatggc aatgaaagcc gagcaagtat gtccattgaa gaaataaaag
    21001 aaattttggg gctaaacact cattaatctt ccttgatttt agtgacatta aaaacccttt
    21061 aaagaagatg acaccaaaag caccttatat aatctcgtta aaacgcttct ttttgaaata
    21121 tctttattga tatagcgatt tgtcatttgc tagtaatgat taaaacattt agaaaaagac
    21181 aagtcataga atcatcaagg aatgctttga aaaataacag tatttaagtt ataatagagg
    21241 atgctacaag aagggagaat caaaaaagga tggtaaaaaa tcagtttcct ttagttgcag
    21301 atggcattgc tattagtgat ccagcaaagc aaatggctct gtatgaaaat gaagatctca
    21361 ttaccaacat ccgtggctat taccaggaca aggagtatga tgatattgct agaaatgagg
    21421 agtttactgc taaagcaact tctcggcagg ccccttctag taagcgcttt tgtagcaatg
    21481 atgaaaaaca ccattatgtc aaagaggcgc gtcaaaaagc aaagcaagat ctcaaggaaa
    21541 aaagacaggc ttatcttgcc aaagaaatgg cctatgttcc taaacaggtt tctaaaaagc
    21601 aacagcctgc ggattcttct ccttctcaaa agcaagcaac aacagaaatg agtcgtttta
    21661 caaaaaagct tcatcaagac aactatattt tagctgagtt gccaaaagaa tataaagagc
    21721 caaaaaatct tcctcaacaa ggaactacaa aaaaaaataa ttacgatttt ttaaaatcca
    21781 gtcaaattta caataataaa gaaatgagac aacaacgaga aaaaacaatt gcgcaagagc
    21841 ttaatcttag taggtttgag gatcttcctt aataaatcta ctcagcgtat gcagatgaca
    21901 aaggagttaa gatgtcaaaa acgtatcatt ttatcggaat taaagggtca ggaatgagtg
    21961 ccttggccct tatgttgcat caaatggggc ataaggtgca aggtagtgat gttgaaaaat
    22021 actatttcac tcaaagagga ttggaacaag cgggcatcac tatcttacct tttagcgagg
    22081 acaatattac gccagatatg gaacttatcg ttgggaatgc ctttcgtgaa aacaacaaag
    22141 aagtggccta tgctttgaga caccagattc ctttcaaacg ttatcatgac tttttaggtg
    22201 attttatgaa atcatttatc agttttgcag tagcaggtgc tcatggtaaa acttctacaa
    22261 cgggtttatt gtcacatgtc ttgaaaaata tcacagacac atcttattta attggtgatg
    22321 gtactgggcg tgggtctgct aatgcgcagt attttgtttt tgaatcagac gaatacgaac
    22381 gccattttat gccttatcac ccagaatact ctatcattac taatattgat tttgaccatc
    22441 ctgattattt cacaggaatt gctgatgtgc gaaacgcttt caacgattat gctaagcagg
    22501 tcaagaaagc tttgtttgtt tatggtgaag atgacgaact aaaaaagatc gaagcacctg
    22561 cacctattta ctattatggt tttgaaaagg gcaatgattt tatcgcttac gacattacgc
    22621 gtactacgaa cggatcggac tttaaggtaa aacaccaagg agaggtcatt ggtcagtttc
    22681 acgtacctgc ttatgggaaa cataacattt taaacgctac agcagtaatt gcaaacttgt
    22741 ttgtagccgg cattgatatg gctctagtgg cagaccatct taaaactttt tcaggtgtga
    22801 aacgtcgttt tacagagaaa attatcaatg acactattat tattgatgac tttgctcatc
    22861 atccaaccga aattgtggca acaattgatg cggctagaca aaaataccca agtaaagaaa
    22921 ttgtagcaat attccagcca catactttta ctcgcactat tgccttatta gaggattttg
    22981 cttgtgcttt aaatgaagct gacagtgttt atttagccca gatttatggt tctgcacgtg
    23041 aagtagataa aggtgaagtt aaggttgaag atttggctgc taagattatc aaaccatcac
    23101 aagtagtaac ggtggaaaat gtctctcctc ttcttgatca cgataatgct gtttatgttt
    23161 ttatgggagc tggagatatt cagctatatg aacattcttt tgaggaatta cttgcaaatt
    23221 taactaaaaa taaccaataa catgtcctgc cttaggcagg ttttctcatt ctaaaggagt
    23281 ttgatatgtt gattagacag gttcaaggat ccgatttgga agttattgct accattgaaa
    23341 gtgacaattt ttcaccacaa gaagcaacga caagagcagt tcttgaagag cgcatacggc
    23401 tgataccaga tactttttta gtggcactta tcgatcagga aattgttggc tatatcgaag
    23461 gtccagttgt cactactcct attttggagg attctttgtt ccacggggtg actaaaaatc
    23521 ctaaaactgg aggctatatt gctattacta gtttgtccat agctaaacat tttcagcaac
    23581 aaggggttgg gacagctttg ttggcggcac taaaagattt agtagtagcg cagcaaagaa
    23641 caggtcttat tttgacttgt catgattact tgatttccta ttatgaaatg aatgggttta
    23701 ttaaccaagg aatttcagag tctcaacatg gtggaaccct ttggtaccaa atgatttgga
    23761 aactttagaa tcacagctga ttcaactgtt ggtttgttaa aaataagtaa caaatgcatc
    23821 atttaattgc aattaggata cttttaagtt ataatggcgg ttgattatat ttaggaggat
    23881 tggctttgac cgattttaag gataaagacc aacaagatca gcagcgaagc tttaaagagc
    23941 agattcttgc tgagttggaa aaagcaaatc aaatcagaaa agaaaaagag gaagaacttt
    24001 ttcaaaaaga gttggaagct aaagaagcag ctaggagaac ggcccagcta tatgctgaat
    24061 ataaaagaca agatgctttt caaaaagagt ctatagcaca taacaataag acagctaaac
    24121 actttcaagc tataaaagat gcggtaatga cttcagaagc gcttaaaccg actttacttt
    24181 ctgaaaaaga aaactcatct ttaaaaacga caaataagag agtcgtgcag gcaaatgagc
    24241 ttcaagagac tgcctctaaa gaatctcaag taccgttaac tattgagaaa ggtcattcag
    24301 tgagacgaaa attaagtaaa cgccaacaga ctgagcgagc tgctaaaaag atttcaaccg
    24361 ttttgattag ctctattatt ataacccttt tggcttttac tctagcagga gcaggctatg
    24421 tttatagtgc tttaaatcct gttgataaaa atagtgatgc ctttgttcaa gttgagattc
    24481 catctgggtc aggcaataaa ttgattggtc aaattcttca aaaaaaaggt ttaatcaaga
    24541 atagcactgt ttttagtttt tatacaaaat ttaaaaactt tacaaatttt cagagcgggt
    24601 attacaatct gcaaaaaagt atgagtctag aagaaattgc tagtgcttta caagaaggtg
    24661 gtacagcaga acctaccaag ccatctcttg ggaagatctt gattccagaa ggatacacga
    24721 ttaaacaaat agctaaagct gttgagcata atagcaaggg aaagaccaaa aaagctaaaa
    24781 caccttttaa cgagaaggat tttttggatt tagtcacgga tgaggctttt attcaagata
    24841 tggtaaaaag atatccaaaa ttattagcaa ctatcccaac taaagaaaaa gctatttacc
    24901 gtttggaagg ttaccttttc ccagcaacct ataactatta caaagaaact accatgagag
    24961 aacttgtaga ggacatgctg gcagctatgg atgctacttt ggtaccctat tatgataaaa
    25021 ttgctgctag tggtaagaca gtcaacgagg tattgacctt ggcctctttg gttgaaaaag
    25081 aaggttcaac agacgatgac agacgtcaaa ttgcaagtgt cttttataac cgccttaata
    25141 gcggaatggc actacaatct aatatagcta ttttgtatgc gatggggaaa cttggtgaga
    25201 aaacaacctt ggctgaggat gctactattg acaccaccat taattctcct tataatattt
    25261 ataccaatac aggtctgatg cctggtccag ttgctagctc gggggtttct gcaattgaag
    25321 caaccctaaa tccagcctca acggattatt tatactttgt ggccaatgtc catactggtg
    25381 aagtttacta tgcaaaaaca tttgaagagc actctgcaaa tgttgaaaaa tatgtgaata
    25441 gtcaaattca gtaaacacaa acatggtgct taagcaccat gtttgtgttt ctgattcgtt
    25501 caaaaaagaa aagagagaaa acaattatgg cagaaaaaac gtatccgatg accctaacgg
    25561 aaaaagaaca acttgaaaaa gaacttgaag aattaaaact tgtgcgtcgt ccagaaatag
    25621 tggagcgtat taaaattgca cgttcgtatg gagatctttc agagaactct gaatacgatg
    25681 ccgcaaaaga tgagcaagct tttgttgaag gtcaaatttc aacgcttgaa actaagattc
    25741 gctatgctga aatcattgac agtgatgctg ttgcaaaaga tgaagtcgct atcggaaaaa
    25801 cagttatcgt tcaagaagtt ggaacaacag ataaagatac ctatcatatt gtaggtgctg
    25861 ctggagcaga tatcttctcc ggaaaaattt caaatgaaag cccgattgct caagctttga
    25921 ttggtaaaaa aacaggtgac aaggttagaa ttgaatcacc agcagctact tatgatgttg
    25981 aaatcatcag cgttgaaaag acaaattaat ccgatgaagc cattatttgc ctaaccacta
    26041 agtgttttgg ttgctaatgg cttttctagt atcttcaaat gaggttgact gttaggaagg
    26101 tcaagtatta atcgcattga ttatgagcca agaggactat agatcaaaga tcgttttcga
    26161 tggttattta ataccaaaag aaaagaccag caccttaatg tactgacccc aaaaagttgg
    26221 acactatatt ttaagtatag gaagtgtata atctaaggaa actgacacta atcattgacc
    26281 gttttgcaag actttattaa tgatttcttg ctttatttta aatgaataag tctgatacgt
    26341 ttccttcctt aacactatcg actccataat gacccatcag tcaaacggta tatctcaaac
    26401 gactacggtt tactccaaat tcattggcta gttgaggcca agaaacacct gattttttta
    26461 atgctaaatt tctagcttat cttcatagct taatttcata caaaaacacc ccaatcgtta
    26521 gatttttttg atatgtcccc tttcactaga tgagtccagt aaaagggggc atatcgtttt
    26581 ttgtctaact tttggagtgc agttcataat tgtgctaggt tttttgtctt tcttaacgct
    26641 ttcgctgttt tccagcatta cgattggatt ttattttttt aggcaagtta gcctgttcca
    26701 tgttttgtga aggcgtaacg tccttacgag aagaagttga ttggtatgct ttaggcgggt
    26761 ttttagcgta ttcttcttta atttgcttat gcaatcgagg ttttaagagg taggttgtga
    26821 ttaactgttg aataatacta aagaaaccac caactagcca gtaaagacca acgccagcag
    26881 ggagtgagaa ggacatgaag atcatcatga taggcatggt gtacatcatc gtcttcattt
    26941 gctcacgttg ttcttctgaa acggccatca ttgataacca tgattggaag aagtaaagag
    27001 cagcgataat tgctgttaac actaaactac ggctaccaag atcaatgctc ataaaggtac
    27061 ttgttgatac tcctttagtg tattgagcgg caaaatacat ggcagaaaag aatggcatct
    27121 ggatgagaag aggtagacat ccaatacctc caagagggtt aatcccatga gcacgctgag
    27181 cagccatcaa ttctgtttgg gcagccattt tttcttcctg actacttgcc tgtttgatac
    27241 gtttgttgat tggttcaaaa acaggtttca agaaggtcat tttctcagat tgataacttg
    27301 ctttccaaga ttgatataga cctagtggta agataagtgt tctaacaata atggttacga
    27361 tgatgatagc cagtccataa cctagtccag cattgtttgc aaagtaatcg ataaagtatg
    27421 acatgggttt tccaagaaat tcccaaatca tacctttggg atttccgtga gcgtctcttc
    27481 ccacacagcc tgtcaaggtg aatagaattg acagtgctag tcctgaaaat aggatgcgat
    27541 ttaatgttag tttcaatgtg tttatccttt aattttaata tacttatcta ttttacagtt
    27601 tttttcagaa attcacaata gttctgaagg ccgattttta attgcctatt tgaaagtcct
    27661 gaaagtcttc aaaattagcc atggtgacat ccacataagt gacttttgcc catttggatg
    27721 gtcctttacg aacttcttga ataaatgtgg ctattttatc tgaatctttt gattgggcca
    27781 gaatttcaac ggtcccatcg ttgttattcc agacacgacc ataaatacct cctatatcta
    27841 gagctaaagt atgagtagcg tatcgaaaac caacaccttg tacacgtcca gaaacaatta
    27901 aacgtacttt ttgcataaga agggcctcct tgcttttatt ataacacata cttagcagga
    27961 aatctcttta gccaattgtc tacttcctgt cacttttact aaagacaaga atacaaaaat
    28021 gagatagtta gtagtactaa cagtaaaaga ggtttgtcta ttttaaaaat agtatcaaaa
    28081 aaaggtcttt taggtttaaa acattgaact gcccccaaat tgaccatcta agaagttgat
    28141 gctttcaaat gctgtcgcgt atggtataat aggcgcatgg ttattacatc aaaagcaaat
    28201 caactaatta agcaaacaaa aaaattattg caaaaaaagc atcgaaaaca gtcctatttg
    28261 attgagggtt ggcacctttt tgaagaagcc caaaaatcag gacaagtttt tcgccacatc
    28321 tttgttttag aggaaatggt ggagcgtcta gcaggtgagc aagaactcgt tattgttagc
    28381 ccacaagtgc tcaaggagtt gaccgactct cctagtccac aaggcattgt tgctgaggtg
    28441 gagataccta agttagcctt tccatcagat tacaaaggga aatatttggt tttagaggac
    28501 gtccaagacc caggaaatct tgggaccatc attcgtacag cagacgctgc acggtttgat
    28561 ggggtctttc tttctgaaaa atcagcagat atttacaacc aaaaaaccct acgttccatg
    28621 caaggtagcc attttcattt accaatttgg cgaacagatg tctatcagct ttgtcgagag
    28681 ctccaagagt ataaaactcc catcctagca acaactctgt caaagaaatc tgttgactat
    28741 aaaagcctca cacatcatga aaggttggct ttggtgttgg gaaatgaagg ccagggcatt
    28801 agtgctgaaa tggcagccct agcagaccaa ctggttcata ttacaatgcc tggtcaggcg
    28861 gaaagtttga atgttgccgt agcagcagga atcctcattt tcagcttgat ttaagctaat
    28921 atgatataat aaccaagtga ggtaattatg ttatgcatta tacagaagat aaagaatata
    28981 tggaacacgt cggtcatttg atcgctcatc ctcgctttca aaaattgagt catattgttc
    29041 agcaccaaca ttcaacgcgt cttgaacatt ctatcaatgt ttcttattcc agttataaat
    29101 tagccaagcg atttggttgg gatgctaaaa gtacagccag agggggctta cttcatgatt
    29161 tcttttatta tgattggcgc gtgaccaagt ttaataaggg tcatgcctgg gttcatcctc
    29221 gtattgctgt acgaaatgcg aaaaagttaa cagaactcaa taaaaaagaa gaggatatca
    29281 ttctaaaaca catgtgggga gcgaccattg cttttccgcg ttacaaggaa agttatattg
    29341 tgacaatggt agataaatac tgggctgtca aggaagcagt gacgcctttg agacaaaaat
    29401 ggtctaatcg acggtttctt cgccgcaaga ccctccaaag tcataatcgt taagtaaagg
    29461 agttattatg aacgatcacg ttatttatac acaatcagat gtgggattaa atcagttttt
    29521 tgctaaaatt tatagtctag tgggaatggg agtgggtttg tctgcttttg tttcttattt
    29581 gatgctttat ccatttagag aaaatttaat ctctattctt gttaatcagc cgatgattta
    29641 ctatggtgct gcgattattg aacttatttt agtttttgta gccagtggtg cagcacgaaa
    29701 aaacacacca gctgcattgc ccatttttct tatctattct gcgctaaatg gctttaccct
    29761 aagctttatt attgtggcat acgctcaaac gacagttttt caagcatttc tttcttcagc
    29821 agctgtgttt tttgccatgt caatcattgg tgtcaagact aaacgggata tgtcaggtct
    29881 tagaaaggct atgtttgctg ctttgattgg tgttgttgtg gctagtctga ttaatctttt
    29941 tattggtagt gggatgatga gttatgtcat tagtgtgatt tccgtgctaa ttttctcagg
    30001 tctcatcgca tcagataatc aaatgattaa gcgcgtttat caagcaacaa atggccaggt
    30061 tggtgatggc tgggctgttg ctatggcctt aagcttgtat ttagatttta ttaacttatt
    30121 tatcagcctt cttcgtattt ttggaagaaa tgattaacat taaaaagtga gtataactac
    30181 tcacttttta atgttaacag gataagtaaa ctgtaagcaa gaaattcgag atagatataa
    30241 gatgaggctg ctaaaagacc tagtgttaat ttgtaaactc tttctttttt tggtatacta
    30301 ttaaggatta aatacgagga gaatatagat agatatgtca acagctattt ggattttatt
    30361 acttattgtt gcccttgggg taggtgtttt tggtgggatt tttattgccc gtaaacaaat
    30421 tgaaaaagaa attggtgaac acccacgttt aacaccagaa gctatccgtg aaatgatgag
    30481 ccagatgggg caaaaaccaa gtgaagcaaa aatccaacaa acttatcgta atatcatcaa
    30541 acaatcaaaa gcagccgtat ccaaaggtaa aaaataaaag ctaattgtta taggggaaac
    30601 ctttgtgata gttagaagtg aacagtcttt gatctcaatg ttcagtagca cttgtcttat
    30661 cagttagatt tgtcaactgt tcagggttta aaaagaaact cagttggcta tttccaactg
    30721 agtttttcat atcgtcagta aggaagtaaa gataatggat acaagaccaa ttgggttttt
    30781 agattctggt gtgggaggtc tgaccgtcgt ttgtgaattg attcgtcagc tccctcatga
    30841 gaagattgta tatattggtg attcagcaag agccccctat ggtcctagac ccaaaaaaca
    30901 aattaaggag tatacttggg agctagtcaa ttttttgctg actcaaaatg ttaaaatgat
    30961 tgtttttgca tgcaataccg caacagcggt ggcttgggaa gaagtaaaag cagctttaga
    31021 tattcctgtt ttaggggttg tcttaccggg ggcaagcgca gctattaaat caacgacaaa
    31081 aggccaggtt ggggtcatcg gaaccccaat gacagtggct tcagacattt atcgcaaaaa
    31141 aatccagcta ttagcaccat ctgttcaagt aaggagtctt gcttgcccga agtttgtacc
    31201 gattgtggaa tcaaatgaga tgtgttcgag tatagctaaa aaaatagttt atgacagtct
    31261 agcaccatta gtcggtaaaa tagataccct tgtactagga tgtactcact atcccttgtt
    31321 acgaccaatt atccaaaatg ttatggggcc atctgttaag ctgattgaca gtggagcaga
    31381 atgcgtccga gatatctctg tcttattaaa ctattttgac attaacggta actatcatca
    31441 aaaagctgtc gaacaccgct tttttacaac agcaaattct gagatttttc aagaaattgc
    31501 ctcgatttgg ctgaaacaaa aaataaatgt ggagcatgtc accttatgag tgaaaaaatt
    31561 tacgaataca aagatgaaaa taactggttt atcggaaaga tgactggtca taatttaata
    31621 tcgggctggg gagtcaaaca cacgacaatc aaaaaaatag atgacctttt agacggtatt
    31681 gccgcaacgt tagattggga aaatcctaaa ggttacgatg tttcggtggt gcgttatcag
    31741 tctcctttat ctctcatcac ctttattatc gacatgataa atcaagagac ccagcgtgag
    31801 attaaggtaa ctccccatgc aggcacgatt ttattaatgg aaaatgcgaa actgcttgca
    31861 gtttatctcc cagaaggtgg agtttcaacg gctacatttt ttgcaactag tgaacaggga
    31921 tttggggata ctattttaat cgctacacga aacgagggaa aaaccaaaga gttccgcaat
    31981 ctgtttgggc agctaggtta tcgtgtggaa aatctcaatg attatccaga attgccagaa
    32041 gtggcagaaa ctggtactac ttttgaagaa aatgctcgct tgaaagcaga aacgatttca
    32101 cgtctcacag gaaaaatggt attagcagac gattcaggac taaaagttga tgccttgggt
    32161 ggtttacctg gggtttggtc agctcgtttc tcaggaccag atgccacaga tgccaagaat
    32221 aatgccaaac tcttacacga attagctatg gtgtttgacc aaaagaagcg ctcagctcaa
    32281 tttcatacga ctttggtagt ggctgctccc aataaagata gtttggttgt ggaagctgag
    32341 tggccaggct atattgctac gcagcctaag ggagaaaatg gttttgggta tgaccctgtc
    32401 tttatcgtcg gagagactgg gcgtcatgct gcagaattag aggctgacca aaagaatcag
    32461 ttgtcgcata gaggtcaagc agttcgaaaa ttaatggagg tctttccagc atggcaagca
    32521 aaacaatcat agtaatgagt gactctcatg gtgaccgaga tatcgttcaa gccattaaag
    32581 acaagtatct tggtcaagtt gatgctattt tccataatgg ggattctgaa ttaaacagct
    32641 cagatcctat ttgggctggt atttatgtcg ttggcggtaa ttgtgactat gatacaggtt
    32701 accctgatcg tttagtaact caattaggca cagttacgat tgcccagaca catggtcact
    32761 tgtaccatat taatttcact tgggataaac tggactactt tgctcaagaa gtagcagcag
    32821 atatttgtct atatggacac ctacaccgtc cagctgcttg gcaagttggg caaaccctct
    32881 ttatgaatcc cggatctgta acacaacctc gcggagaaat taacgagaaa ttatatgctc
    32941 gtgttgaact aactgatact caaattaaag ttgattactt tacaagagat cataaacttt
    33001 actcatcact gtctaaggaa tttaaacgat gatcgcaaaa gaatttgaaa cgtttttaat
    33061 gagtcattta gataattatc ttatcccgga acaagacttg gctattttta tcgacacaca
    33121 taatgcagac catgtgatgt tactattggt gagcaatggt ttttctagag ttcctgttat
    33181 cacacgggag aaaaaatatg tgggaaccat tagcatctca gatattatga tgtaccaatc
    33241 taaaaggcag ctgacagatt gggaaatgag tcaaacagat attggcgaga tggttaatac
    33301 caaaattgaa accattagca ttacttctag cctaacagaa attatgcaca agttaattga
    33361 tttcccattc ttaccggttg tagatagagc gaaccgcttt gttgggatta ttacgcgaaa
    33421 atctatatta aaggctgtca acagtctttt acatgatttt acagatgatt acacgattat
    33481 aaaaaaatga agagttatat tgaacctttt attgctagca aggctttatc tcaaaatagt
    33541 caaaaagctt atcgatacga tttgcaacag ttttgtcaac tggttggtga acgtgtcaat
    33601 caagacaaac tattacttta tcaaaatagc atagctaacc taagtttatc tgcaaaaaaa
    33661 cgcaaattat caacagctaa tcaattctta tattacttgt atcaaataaa gtatttaaat
    33721 agttattttc gattaacaga taccatgaaa gtgatgcgga ctgagaagca gcaagctgct
    33781 attattaata ctgatatttt ctatcaaaaa accccttttg tgtgggggca attaatcagc
    33841 ttattaatat tggagctggg tttaactccg agtgaagtag ctggtattga agtagctaac
    33901 ttggatttaa attttcaaat gttgacgcta aagactaaaa aaggtgtgcg tgtattacct
    33961 ttgtcgcaaa tactaatccc ttttttagaa caacaactcg ttggcaaaga agtctatctt
    34021 tttgaacatc gaggcatccc tttttcaaga caatggtttt ttaatcacct aaaaaccttt
    34081 gttcgaagta ttggttatga aggattgact gctcaaaaat tacgagagca atttattttg
    34141 aaagaaaaac tagctggtaa atcgataata gaacttagcg atattcttgg gctgaaaagc
    34201 cctatgacct tagaaaaata ctacaaatca taatggatat aaaacttaaa gattttgaag
    34261 gtcctcttga tttactcttg cacttggtat cacagtacaa agttgacatc tacgaggttc
    34321 ctattgttga ggttattgag cagtacctta attatattga aaccttacag gtaatgaaac
    34381 ttgaagtggc tggtgactac atgctgatgg caagtcagct catgttaatc aaaagtagaa
    34441 ggctattacc aaaggtggta gaacacatcg aagaagaaga tttagaacaa gatctgttag
    34501 aaaagatcga agaatatagt cgctttaaag cagttagtca agcgttagcg aagcaacatg
    34561 accaaagggc aaaatggtat tcaaaaccta aacaagaatt aatttttgaa gatgctatct
    34621 tacaggaaga taaaacggtg atggatctat ttctagcttt ttcaaacata atggcagcta
    34681 aaagagcagt tttaaaaaac aaccatacag tcatcgagcg tgatgattat aagattgaag
    34741 acatgatggc ttctattaaa cagcgactgg aaaaagaaaa cgttattagc ttatcagcga
    34801 tttttgagga atgccaaaca ttaaataaag ttatcagtat atttttagct agtctagaat
    34861 tgattaagct tcacgtggta tttgtagagc agctctctaa ttttggggct atcatactac
    34921 gaaaggagaa gaaatgacgt atttgtcaca gatagaagcg ctgctatttg ttgcaggtga
    34981 agaaggatta agtttgcgac atctagcgtc tatgttatca ttaacgccaa cagctcttca
    35041 acagcaatta gaaaaactct ctcaaaagta tgaaaaagat caacattcta gtctttgctt
    35101 gatagaaaca gctaatacct acaggctagt aactaaagaa ggttttgcag aactcttgcg
    35161 tgcttatgcc aaaacaccaa tgaaccaaag tttatctcgt gcgagtttag aggtcttgtc
    35221 tattgttgca tacaaacaac ctattacacg aatagaaatt gatgacattc gaggggtaaa
    35281 ctccagtgga gctttgagta aattattggc atttgatctc attagagaag ctggcaaaaa
    35341 agatgtggtt gggcgtcctc atttgtatgc gacaacagat tattttctgg actatatggg
    35401 aattaatcat ttagacgaat tgattgaagt atctgccgtt gaaccagcag atgaagagat
    35461 tgctcttttt aggacacaag actgagtttt gcttgtcatc aaatagcaaa tggcgtttac
    35521 aaaaggattt gttttaaggc gttttagttg aaaaaagcct taaactaggg acaacaaaaa
    35581 gttgctcaaa agaaagaaga atataatgcg aattaacaaa tacattgccc atgcgggtat
    35641 tgccagtcgt cgtaaggctg aagaattgat taagcaaggc ttagtaacct taaatggtca
    35701 ggttattacg gatttagcaa ccactgtaaa atcgggagac gtggttgaaa tagaaggaag
    35761 tcctatttac aacgaggaaa aggtttacta tttgcttaat aaacctcgtg gcgctatctc
    35821 aagtgtgtca gatgacaaag gccgtaaaac ggtactggat ttactacctc aggttaaaga
    35881 acgcatatac cctgttggac gattggattg ggacacatca ggggtcctta ttttaacgaa
    35941 tgatggtgat tttacagata ctatgattca tcctagaaat gaaatagaca aggtttatct
    36001 ggcgcgtgtc aaaggaattg caacgaaaga aaatcttcgt cccttaactc gaggaattgt
    36061 tattgatggt aaaaagacca aaccagctcg ctacaatatt gttcgtgtag aagctgataa
    36121 gaatcgatcg attgtagaat tgactatcca tgaagggcga aaccatcagg ttaaaaaaat
    36181 gtttgagtct gtggggcttt tggttgataa attatctcgt acacgttttg ggaccgtcga
    36241 cttaaaggga ctaagaccag gagaagcaag acgcttaaat aaaaaagaaa taagtcaact
    36301 gcataactta gctaatacga aaaaatgatg aaaaaactat taatagtatc tgttaaagct
    36361 tatcaaaaat acatttctcc tttatctcct cctagttgtc gctacaagcc aacctgttca
    36421 gcttatatgt taactgctat tgaaaaacat ggtactaagg gaatactaat ggggatagca
    36481 agaatattaa gatgtcaccc ttttgttgct ggtggtgtcg atcctgttcc agaagatttt
    36541 agcctaatga gaaataaaaa cacgtctaaa aacgctgaaa aggcatgact ttatcaaaaa
    36601 atggcttgac attgatagct gaagtgttat aatggaaaac aagcattcgt tggtttaaat
    36661 caaacctgtt atgatttaag ttagcgagtc accatcaacc acattgtttg ctgagcatga
    36721 ctccgagcag tgtggttttt taaattaaaa agagtaaatg agattatgac gacaaaagaa
    36781 cttatcaaca aaaacgacaa agtaaaaaag gctcgcaatc atattgtcct gtttcaaccc
    36841 caaattccac aaaatactgg taatattgct agaacatgtg ctgcaactaa tgcaccttta
    36901 cacattatta aacccatggg atttcctatt gatgaccgca aaatgaagcg tgccggcttg
    36961 gattactggg acaagttaga actgcatttt tatgatcatt tagaacagtt tatcaatcaa
    37021 tgtcacggac agcttcacct gattagcaag tttgcagtta ataattattc tcaagctact
    37081 tatgctgatg gcgattccca ttatttcttg tttggacgag aagatacagg cctgccagaa
    37141 gattttatgc gagaacatgc tgaaaaagca cttcgtattc cgatgaatga tgagcatgta
    37201 cggagtttaa atgtctcaaa tacggtttgt atggtgattt atgaagccct tagacaacag
    37261 ggctttcaag ggttagagtt aaagcatatt tacgaacacg ataaactaaa ataatggact
    37321 ttttaaaagc gtcagttttt ggcaacatgc gttgtcagta gctgacgctt ttaaaaattt
    37381 agtcaaagct attgctatct aggtttaaat ctgctatgct agaagtgtct tcagggcagg
    37441 gtgtgattcc cgaccggcgg tgactaggta actagaagtc cgcgagcgca agctgatgtg
    37501 gtgtaactcc acaaccgaca gtatagtctg gatgagagaa gaccgggctt tttgtgtagg
    37561 cttattttag ctacacaata acgacggaac ttctttcaat ttgaaaaaat tggaggaatt
    37621 ttttaatgtc aaaaacacat aaaatgatta tgattggtat actttctgcg atatcatttc
    37681 tattgatgct agttagcttt gccattattc caggcgcagc ttttttaaaa attgaattta
    37741 gtattattcc tgtcttattt ggtttaatga ttatggactt aaagagtgct tacttgattt
    37801 tgttattgag gtccttattg aaactctttt tgaacaatcg tggggttaat gattttattg
    37861 gtctcccaat gaatattatc gctatcgctt tatttgtgac ggcttttgcc ttagtttgga
    37921 accgtcaaaa aacgcttagt caatatgtat ttgctagctt actaggcaca gggttattaa
    37981 cgtttggcat ggttgttctc aattatactt ttgccattcc tttatatgct atatttgcaa
    38041 atattgatat cagagcttat attggtgtta ctaagtatat gatgactatg gttattccgt
    38101 ttaatcttgt tgaggggttg atatttgcaa ttacctttta ttttgtgtat attgcaagta
    38161 aaccaatttt agaaagatac ttacactaat atgatgacaa acaaacaaac tcacttttta
    38221 atagcttctt ttgcattgct aatctttgtt ataataggct atacggtcaa attttttcct
    38281 gaacgtttgg cacttctaga taatactatt caagcagaga ttagaggcaa ccttcctata
    38341 gtgctgacgc aattctttag gggtgttact gtatttggaa atgtcatgac acaagttttg
    38401 ttagttattg tgtcggtcct agttttattc tttatgaagt ggaaaattga agctttgttt
    38461 atcttatcaa atggtgccat tgcagcattt ttgattacga ctctcaagct cttttaccaa
    38521 agaccaagac ctgctattga gcatttggtt tatgctggtg gctattcgtt tcccagtggc
    38581 catgcgatgg gtagcatgct gatttttggt agcctattga ttatttgtta tcagaggcta
    38641 cattcaaaac tattacaatt tgtgactagc atgatcttta taatactaat tttattaatt
    38701 gggttatctc gtatttatct aggagtacac tacccaagtg atattttagc aggctttgta
    38761 ttaggatttg gtattttgca ctttatttat cctttttata agcaaaaacg atttgaatgg
    38821 cgatttcttt tgaaacaaga ctaatttttc ttgagaagac atagggctta tttaaatcat
    38881 ttagaataac attggcaagc atcacccctt gattgccagt gttattttag ttttagttag
    38941 atgatgtgag ctgataatta gtaaatacgt ttagataaaa aacgattaaa gtgctatgta
    39001 acgtcagaaa agacatgatt tttgttgatc tgtaatcatt aagtaagctg atgtagaagt
    39061 agtgaattct aatgactgag ctatcttttg catagatgac tatcttgcta gtaatgattt
    39121 gtgctacaat agaaacatga aaaaaagata tcagacatta aacgagcatt accgtcaact
    39181 atttggcgca aaaatgttta aagttccgat tgatgccggc tttgattgtc ccaatcgtga
    39241 tggaacggta gctcatggag gatgtacttt ttgcacagta tcaggttctg gagatgctat
    39301 tgtagcacca gatgctccaa ttaaagagca attttataaa gaaattgatt ttatgcaccg
    39361 taaatggcct gatgtcaatc gctatttggt ttattttcaa aactttacca atactcatga
    39421 tacagttgat gtgattcgcg accgttacga acaagctatt aacgaacctg gagttgttgg
    39481 tattaatatt ggcacacggc ctgactgtct accagatgat accattgctt acttagcaga
    39541 attatcagaa cgcatgcatg tgacagtgga gctaggctta cagacaactt atgaagaaac
    39601 ttctaggcta attaacagag cacattctta tgatttgtat aaagaaacgg tgaggcgact
    39661 aaggcattac cccaacatta atattgtctc gcacttgatt aatggattgc caaaagaaac
    39721 tcatgacatg atgcttgaaa atgtgagacg ctgtgtaaca gataatgaca ttcaaggaat
    39781 taaattgcat ttgctgcatt tgatgaccaa tactagaatg caaagagatt atcatgaagg
    39841 acgcttaaag ttgttgagtc aaaaggacta tgtttcgatt atttgtgacc agctagagat
    39901 tatccctaag catattgtga ttcatcgtat tacaggagat gccccacgag atatgttgat
    39961 tgggcccatg tggagtctaa ataaatggga agtattgaat gccattgata aagaaatgga
    40021 aagacgagga agctttcagg gctgtaaagt tgactaattt gtctcatttt gacggatttg
    40081 gataggagaa aagtgatgct aaaacgcccc attcatctct cgcatgattt tttagcggag
    40141 gttgtggata agtctagtgt ggttgttgat gccacgatgg gtaatggaaa tgatactgcc
    40201 tttttggcac aattagctaa aaaggtttat gcttttgatg tgcaagaaca agctattaga
    40261 aaaacaagtg agcggttagc gcagctagga ttatcgaacg ctgagctgat tttagcgggt
    40321 catgaagcgg ttgatcagta tgtgacagag ccggtgcgcg cagcgatttt taaccttggt
    40381 tatttgccca gcgcagataa atctattatt accttaccaa ataccacact ccaagcttta
    40441 tcaaaactac taacgttatt aatggtaggt gggcgtattg ctattatggt ctattatggg
    40501 catgatggag gcagtttgga aaaagatgct ttattagatt ttgtcaagca attagaccaa
    40561 agaaaggttt ctgcaatgct ttatcagcct cttaatcagg tgaatacccc acctttttta
    40621 atcatgttgg agaaattagc agacgtttag ctcttatccc tgtgtcaagt ctactagaag
    40681 ttctgcgatt atttcggtgg aaaaagaaga aatcccatag gctttggcta taaatgtgat
    40741 ataatgaagt cactatatta tttttttagg agttacatta acatggaaga ccctgtgagt
    40801 cagcccttag tgattcaatt tttattgtta gttgttttaa ccttgttaaa tgcttttttt
    40861 tcagccagtg aaatggcctt agtttctctc aatcgttctc gggtggaaca aaaagcagca
    40921 gacggtgata aaaaatacgc tcgtttgttg cgggttttag aggaacctaa tcatttttta
    40981 tcaacgattc aagttgggat tacctttatt agtttactat caggagcaag tttatcagct
    41041 tctttgggta aggtgatctc aggttggcta ggtaattcag cgaccgcaag gacagctggt
    41101 actatcatct ccttggtttt cttgacttat gtctctattg ttttaggaga attgtatcca
    41161 aaacggattg ccatgaacct caaagacaag ttggcgattg tttcagcccc tattatcatt
    41221 gggttaggga gactggttag tccctttgta tggctcttat cagcttctac taatttgctg
    41281 agccgactta cccctatgac ctttgatgat gcagatgagc aaatgacacg tgatgaaatc
    41341 gagtatatgt tatcaaaaag tgaggcgacc cttgatgctg aagaaattga gatgttgcaa
    41401 ggagttttct cacttgatga aatgatggcg cgtgaagtca tggtcccaag gaccgatgct
    41461 ttcatgattg acattaacga tgatccgctt gaaaatattc aggaaatctt aaaacaaagt
    41521 ttttcacgca ttcctgttta tgatgtggat aaagataaaa ttatcggtct catccacact
    41581 aagcgtctct tggagtcagg tttccgccag ggatttgatc agattaacat gcgaaaaatg
    41641 ttacaagaac ctctttttgt tcccgaaacc atttttgtag atgatctctt acgccagctg
    41701 cgcaataccc aaaatcagat ggctattttg ctagatgaat atggtggtgt ggcaggactt
    41761 gtgactttgg aagacttgct tgaagaaatc gtcggtgaaa tcgatgatga aaccgataaa
    41821 gcagaacaat ttgttcatga gattggagac aatacctata ttgttgttgg taccatgact
    41881 ttaaatgagt ttaatgacta ttttgatacc gaactagaat cagatgatgt agataccatt
    41941 gctggttttt atttgacagg tatcggaacc attccaagcc aggatcaaaa agaagcctac
    42001 gaaatagata acaaagacaa acatttagtt ctaatcaacg ataaagtcaa agatggccgt
    42061 attacgaaat taaaattaat cctgtctaat atagaacaga ttattgagga agactaggct
    42121 ccatgcctag tctttttgat gaaaagtgtt ataatgaaaa tgtaaaaacg tttacaagta
    42181 atgaaggaga tgcaatgact gagaaagatt atggacaagt aacagggatg gtgcattcga
    42241 cagaaagttt tggttctgta gatggtccag gcattcgttt tatcattttc ttgcaaggct
    42301 gtaaattacg ttgccaatat tgccataatc cggatacttg ggagatggaa accaacaatt
    42361 caaaaatcag aacagtcaat gatgtcttga aagaagccct tcagtacaaa catttctggg
    42421 gaaaaaaagg aggaattacc gtatctggtg gtgaagctat gcttcaaatt gattttatca
    42481 ctgccttatt catcgaagct aaaaaattag gtatccatac cacactagac acttgtggct
    42541 ttacctatag accgacacct gaataccatc aggttcttga taacttattg gcagtgaccg
    42601 atttgatttt attggacttg aaagaaattg atgaaaaaca acataaaatt gtcacgcgtc
    42661 agcctaataa aaacatattg caatttgcta ggtatttatc cgacaagcag attcctgtct
    42721 ggattcgaca tgtcttggtt ccaggcttga ctgatattga tgatcatttg acacgattgg
    42781 gtgagtttgt caaaaccttg aaaaatgtcg ataaatttga agttttgcct taccatacca
    42841 tgggagaatt caagtggcgt gagctaggta ttccttatca gttagaaggt gttaaaccac
    42901 caactaagga acgcgttcaa aatgctaaaa atctgatgca gaccgaatct tacacagaat
    42961 atatgaatcg gattcatcag tcataagagc tgttataggg ttgaggtttc ctcaactttt
    43021 taaagtatta taagcagatc ttttgtaatc ttcctttgat tttagggggt aattttgcta
    43081 aaataaaatg aataaacaaa atgaggtaga atgattatgt caaaaatttt agtttttggt
    43141 catcaaaatc ctgatacgga tgctattgca tcatcttatg cttttgatta cttatcccaa
    43201 aaagcgtttg gtttggatac tgaagtcgta gctttaggaa cacctaacga agaaacagct
    43261 tttgcccttg attattttgg tgttgaggca ccacgtgtgg tagagtctgc taaagcacaa
    43321 gggtctgaac aagtgatttt gacagaccac aatgaattcc aacaatccat tgcagatatc
    43381 cgtgaggttg aggtatatgg tgtggttgat caccaccgtg tggctaattt tgaaactgca
    43441 aatcctttat acatgcgtgt ggaaccagta ggatcagcct cgtctattgt ttaccgtatg
    43501 tttaaagaaa atggcattga ggtgccaaaa gcgattgcag ggatgctctt atctggtttg
    43561 atttcagata ccttgttgtt gaaatctcca acaactcatg tgtctgatca ccttgtcgct
    43621 gaagaattgg cagagctagc agaagtgaac ttggaagact atggaatggc tcttttaaaa
    43681 gcaggtacaa accttgcaag caaatcagaa gtagaattga ttggtattga tgccaaaaca
    43741 tttgaattaa atggcaatgc tgtacgcgtg gcacaagtga acactgttga cattgcagaa
    43801 gtcttagaac gtcaagaagc tattgaagca gccattaaag atgccatggc tgcagaaggt
    43861 tattcagact ttgttttgat gatcacggat attgttaact caaactctga aattctagct
    43921 attggagcaa acatggacaa ggttgaagca gcctttaact tcacccttga caacaatcat
    43981 gccttcttag caggtgccgt ttctcgtaaa aaacaagtgg ttccacaatt gacagaaagt
    44041 tttggagctt aatgaaaaga cgctttaaaa ggacttggtt tttttgacca agtccttttt
    44101 tcactcttct atgctataat agaaggtatg ataacaatat ttcattcaga taaactcacc
    44161 agacaacctt tctttcaaga ccttatcaat tatctcgacc agcatgatca cgttatatta
    44221 cgagagatca aaaaagcctt tcctaatgtg acaggtattg acaaggccat cgaaagctat
    44281 gttcaagctg gctatattcg ccgtgaaaat aagcgttatg gcatcaacct tcctttggtg
    44341 agttctgatc agcagctggc cttagacact atgctttttg tggacacctg ttcagctatg
    44401 tatgaaaata ttttagcggt tgtttttgag actcagctaa caaaccaaac caatcacgtg
    44461 atgatcaagg aaaagaccaa catcacgaga agtcagttga ctctagctaa ttatttttac
    44521 cgtctcaaaa gaggtgagaa gccatcagct gagcagatag acttgtatga cctcttgggg
    44581 gatgtgaatc aggaatatgc ccttaaatat atgacaactt ttttgcttaa attcacgcgc
    44641 aaagaccttg tgatgcaaaa acgtcctgat atatttgtgg aagctctggt tacacttggt
    44701 tacctaaaac aagtggagcc tacaacatac cagctgctaa tgaccttgga taaagaaagt
    44761 ttaaccttta tagcaccata gaaaagagct aggaacaagg actgttctta gctttttgag
    44821 tgcttttatt ttgtttttgc cattaagtaa agaaaataag gggctccgat aagcgacact
    44881 aaaatccctg tctggatatt actgccaacc agatagactc gtcctactgt atctgcaaca
    44941 agaagtagga tcattccgat taaaatacta atgggtattg ttattttatg attactccct
    45001 accaagtatg tactaaagtg tccagctatt aagccgataa aactaatatt accaaccaaa
    45061 agaacactca aagcacctaa gccagctgct aagacaagta tcaaacgacg cttacggttt
    45121 aaagggagtc ctagtccaat agcagtatta tcagctaatc ccataatatt aaggaaatgg
    45181 gcttgactat aagtcaatag ccaaaaacac aataataaag gagcgatgac actcagagta
    45241 ggccagtcgt ctcctattaa ttggccgcta agccaattga tgaccaaatc gactttgtag
    45301 cgattaatat gaccaacaag agcaaccatt agacttgaaa gcatggtagt aacagctaca
    45361 ccagtaagaa ttaaccgtgt aggatcaatt tgtccttgtt ttgtcaaaga tagccaataa
    45421 acgctaaagg ttgttacaac agcaccaagc atggcaaata aaggcaagta atggctgata
    45481 tgagcttgct caaagaaaga aaaggagacc acaatagcca aagagatacc tgctcctata
    45541 gtaataccta atacaccaga gtcagctaag ggatttcgtg ttaaacgttg taataatagc
    45601 cctgataaag caagagaacc gcctcctaga caagcagcta gaattcttgg cagacgaatg
    45661 ttaataacga tgaaagatat ggcatggcta gattttccca aaaacacatg gacaaggtcc
    45721 aaaaaagata gatgagattc tccaagggac aaagctaatc ccataagact tacaagtagc
    45781 aggatcaggc tagtatataa ccttaaaagg tgtctttgag atttcaatag cggcctcctc
    45841 ttcttattaa ccataaaaag catggaaaac caattaagct aaccaatgcc ccaagagggg
    45901 tttcataagg cggattgaga ttacgacaag caatatcaac ccataccatg aaactagcgc
    45961 cagaaactgc acaaagcggc aaaaagtatc ggtaatgatg aggtgtaaag tgtttcataa
    46021 gatgaggaat gactaagccg ataaaagaaa tgcttccagc aatggctaca gctgctgatg
    46081 ataaaataag aaccaaaatc ataaagactg cactgatcaa gttggtcttt tgcccaagag
    46141 cttttgcctg agactcactt agactaagga cggtcaggtg ataagataat agctgggcta
    46201 aacaaagact aagaataata agaggagcaa tatagccgat catttgccaa ttgaccccga
    46261 caagacctcc tgcttgccag ccgataacag catttgtcag atgatagtaa ttggtaatac
    46321 cttgacctaa tgctgatagc agtatggaaa ccataactcc tgctaagaca aggcggagct
    46381 gatggtagct tttgccagat tgataagaaa ggccaaagac taaggttgct gccaggctag
    46441 accctaacaa cgaaagcaga ataatgaggg aatagtgcaa gtgaggtaca aacgcataag
    46501 ccagtaccaa ggctagtcct gctccagcat taatgcctaa aaggccaggc tcagcgatag
    46561 gattacgtgt gattgcttgc atgatagttc cagaaacagc taacgcagag ccagttaagg
    46621 tagcaccaaa aagtctcggt aatcgtatag cgagtaaaac attggcttta tggccgtttt
    46681 gctttccaaa taaaatagag ttaagatcct gatgtgataa agcaacggct ccaaagcgca
    46741 gatgacagta aatagcaaca cataagctaa aactaagtag cacaaagaca agccaaaaga
    46801 tatgtgactt agtgattatc tgcgtttttt ttaagcaaaa acgctcgaga cacaaaaagg
    46861 gatgcttagt tttcacttga taagattgcc tctcgtaaag tctctaattg atattctaaa
    46921 gaaagaggat cagtaaagta aaagacattg gcattgactt tgatcacatg gtgttttttc
    46981 acagctggaa tggattgcca tagcttgctt tcatatagag cagaaccagt cttatcatct
    47041 tctgcagcaa cgacgacata atcaccaata tagtctggca agacttcctg cgacaatgac
    47101 aggtaacctt gtttaaaaac ttcagtcttc actttttcag gagcatcata atggaaagct
    47161 tgatggataa cctctccgcc acgtccccag tctttaccaa aaaggtaaac gtctttttca
    47221 taaagcccca taatagtaaa ggttgctttg tcacctgtaa cggccttgac ttctttttca
    47281 taagctgctg ttttagtttt ccaatctttt aaccatttct tggctttgcc ttctttgtta
    47341 aagatgcggc cgaagtcaga taacacttgt aaataatcac gtttgcggta ttcaatcgaa
    47401 ataacaggcg caatttctgc caattgttta atattttctt ctgtagagcc aacaacaatg
    47461 aggtcaggct taagcgttgt aatggcttct aaatcagttg cagcaacttg ctttgctttt
    47521 tttacagtct ttgctaagat ggggtttttc ttgtcataag aagtaacccc gactagattc
    47581 atatctaatt ttttgaggta accagtgtaa gttgaagcca gactgactac tcgcttaggc
    47641 tgtttaggaa tatctccgta ataagtcaca ccagcaattt gaggcatagg cgataaactt
    47701 tttgaggcgg tgttagaatg attagttgct tgattaccac aggcgattaa ggtaatagtt
    47761 gtcaaacaga gtgttaatag tagagttaat tttttcatgt atgctaattc tcctagaata
    47821 aagttattga agctgataag tcagcactat aggacagttg tggataggat catcaaccag
    47881 taccggtttg atttggaaga tgtcttgaat gataggtgaa gtcataacat ctgctatagt
    47941 accagtataa tggatcttgc catgtttcat agcaatcaga tgatccgaat agcgggctga
    48001 aagattgagg tcgtgaagaa ccataacaat agtcttgccg gcatccttat ttagggattt
    48061 aacgagctct aggatttcta gttgatgatt taaatctaaa taagtagttg gctcatctaa
    48121 aaaaatagta tcagtgcctt gggctaatgc cattgcaagc caaacccttt gacgttgccc
    48181 cccagaaaga gcatcaacag gttgatcagc ataggccatg acattggtgg cttgtatagc
    48241 ccaatgaatg gcttttttgt cagctggact aagatttcca aagtaagatt gatggggaaa
    48301 acgtccgtaa gaaaccaatt cgtaaacagt tatgccgtta gttgcttctt gaacttgagg
    48361 taaaagagct agttttttag caacctcttt agtttctaat gtggcaatgt tttgcccatt
    48421 taaatacaca actccctgtt ttggtggtaa taatcttgtc agtgctttta ataaactaga
    48481 cttcccacag ccattggcgc caataatggt cgtaatttta ccttcaggaa tataaaacga
    48541 taatttatcg atgatggtac gctgttcata ggcaattgtg aggtcttcag cactaattgt
    48601 tgtcatatgg ttgcctcctt tctaaaaaga gtcaaagggt tgatgaaagt gcacattaaa
    48661 gtgttcatct gcccatttta aaatagttga gaagtagctt attttctcaa aatcttcact
    48721 attatagcat agaaaaaagg cttaacaaag ccttttttga tgtgattttt aggatgtatt
    48781 aaaaaagaaa tattagttta gaatgattaa aaacaatagc gataatcatt ctaaactaac
    48841 gtcgacatac tctagaggta gtgtttggcg attgctaaat caccagcata agctacttgt
    48901 tgtcctttaa caatttgata agcatctgcg cctttcccag cgataatcac agcatctttt
    48961 gctccttggc aaagagacat ggctttttga atagcttgct cacggtcact aataatctct
    49021 accgggcgtg cgatatggct ggcaatttct tttgaaatat cttctggatc ctcgaaatta
    49081 gggtcatcag cagtgagaat gacggtgagg tttggatgct gatgaatcac cctaccaaaa
    49141 tcagctcgtc tgctctcgcc tttatttcct ggtgcaccta aaatcaacat taatttgcct
    49201 gtctgatgtt cttccacaac gctgagtaat ttttctaagc tgtcaccatt atgggcataa
    49261 tcaacaaaaa ctttagcatg atttgtcata gtgaggactt ccatacggcc tgggacgcga
    49321 gtcttagcga tacctttttg aatatcagct aggctagcac ccaaacgaag gcaggcaagt
    49381 ccagctgcca tagcattttc ttggttaaaa tggccaatta attgaatgtc ataatggcca
    49441 gctaattgtc ctttagcttc aaaggagaaa gcttggctag tggtgatttg attgtcagac
    49501 aagggaccat aaaacacatg ctcttgatcc gctacctgat ccgctagaaa tgaaaaatga
    49561 tccatcccgc tattgatcac aacagctctg ctattctcca ttaaaagacg cttatgatag
    49621 aaataatctt caaacgttgg atgctcgatt gggccaatat gatcaggact aatatttagg
    49681 aaaaccccaa catcgaacgt aagcccatag acgcggccaa caagataagc ctgactggat
    49741 acttccataa tcaagtgggt catgccattt ttaacacact cggccatcat agcaaaaagg
    49801 tctagacttt caggagttgt caattgtgat ttgaagaaag tttgaccgtc caaggtagtg
    49861 ttcatcgtag acaacaaggc aggtttataa gattctttca aaatatggta ggcaaaataa
    49921 gcagctgttg ttttgccttt tgttccagta aaccctaaga gtttgagctg atcctgagga
    49981 ttatcataga aagccatagc gattaaactc atggcctttt taatatcagt gaccaaaaca
    50041 acgggaatat caagcttata atctacttct gagacataaa gaggaagtcc cttagcgatt
    50101 gcttctttaa ggtattcagc cttgaatgta gctcccttag caaaaaaaag ggtcttgcca
    50161 tctacttgac ggctatcgta actcaagcgc tcaaaactta agccttgata atggtagtgg
    50221 tatccgtcgg catcaagaac ctcacgaaaa ttatggtctt tttttaaaat gtctaataat
    50281 tgttcaatgg ttatcatacc cttattttat ccttaaagtt cttgttttac aagtcacaag
    50341 caaaagttta taataaagct aatagaaact aggaaagaat agatatgtcg acagaaaaaa
    50401 aacaactgac acaagaagaa ttgatggttc agggagcagc ctggtccaca gctggtaatt
    50461 tcattagccg acttttagga gtgttataca ttattccctg gtacatttgg atggggcaat
    50521 acgcgattca agccaatgct cttttcaata tggggtataa tgtttatgct tattttttat
    50581 tgatttcaac cacaggactt aatgttgcga ttgctaagca agtcgccaaa tacaattcta
    50641 tggggcagac ggagcatagc taccaattga ttcggagcac gttaaaactc atgcttggtt
    50701 tgggtgttgt gttttcggca attatgtatc taggttctcc cttatttacc agtttatctg
    50761 gtggggatgc taagcttatt ccaattatgc atagtctttc cttggctgtc cttgtctttc
    50821 cagtcatgag tgtcattcga ggcatttttc aggggcacaa taacattaag ccctatgcca
    50881 ttagtcagat tgctgagcaa ttgattcgag tcatttggat gcttctgacc accttcctta
    50941 tcatgaaatt aggttctggt gattacgctt cagcagtgac tcagtcaact tttgcagcct
    51001 ttatcgggat ggtggctagt atgggtgttc tgggttacta tctttggaaa cagggacttc
    51061 taacagctat ttttagtaag ccagatcata ccgtttctat tgatatcaag ggcttgttgc
    51121 ttgaaacctt aaaagaatcc atccccttta tcgtgacagg aagcgctatt caagcctttc
    51181 agttgattga ccagtggtcc tttgtgaata ccatgagtct gtttacagat tatagccgct
    51241 ctcaattatt ggttttattt ggttatttta acgctaatcc tgctaaaatt accatggttt
    51301 taatcgctgt agcagcttct atcggagggg ttggtattgc tttgttgaca gaaaattatg
    51361 tcaaaaaaga catgaaagca gctgctcgct taatcattaa caacattgaa atgttagtga
    51421 tgtttttgtt acccgctctt actggggcaa ttattttagc aagacctcta tattctgttt
    51481 tttacggagc tagcgaggat cgtgccattc acctctttgt ggcggtcctc ttccaaacct
    51541 tgctactggc gctttacacc ctcttttcac cgatgcttca agctcttttt gaaaatcgaa
    51601 aagcgattta ctactttgcc tatggtatct tgattaagtt agttttacag ataccgctta
    51661 tttatttgct acatgcttat ggtcctttac tagcgacgac gattgcttta gtggtgccga
    51721 tttatttgat gtatcgacgc ctatatcagg ttactcattt taaccgcaaa ctgttgcaaa
    51781 aacgtttatt attaaccttg attgaaacct tactaatggg actggtcgtg tttgtggcca
    51841 actggctatt gggctatgcc tttaaaccga caggccgctt gaccagcctt ctttacctcc
    51901 tcattattgg tggcttggga atgacggttt acacggcact caccttgctg acgcatcaac
    51961 tggataaatt aattggtagc aaagctagtc gccttcgtca gaaattaggc tggcattaat
    52021 ataatgacac caagaacgat ttcgtgagtg cttatcgaaa tcgttttttt cttaatgatc
    52081 tcgcttagta aaggtagctg ataaaaaatc aggatttccc aagcaaacta ctgatatttt
    52141 tggtataatg ataaaagtgt aaaaaaatag aaggaagtat atcaacatgg gaaaatgtca
    52201 agttatttcg catccgctta tccaacacaa actttcaatc ttacgtcgtc aaaccacatc
    52261 aactaaagat tttcgtgagt tggttaatga aattgcgatg ctgatgggat atgaagtgtc
    52321 acgcgacctt cccttagaag atgtcgacat ccaaacgcca gtctcaaaaa cggtccaaaa
    52381 acagttagct ggtaaaaaat tagctattgt tccgattctt cgtgctggta ttgggatggt
    52441 tgatggcttg ttaagtttgg taccagctgc aaaagttggc catatcggca tgtaccgtaa
    52501 tgaagaaact cttgaacctg ttgaatacct cgttaaacta ccagaagata ttaaccaacg
    52561 acaaattttc cttgttgatc caatgttggc aacaggtggt tcggctatct tagcagtgga
    52621 ttcgttgaaa aaacgtggtg cagcaaatat taagtttgtc tgtttagttg cagcaccaga
    52681 aggggtgaaa aaacttcaag aagttcatcc agatattgat atcttcacag cagcacttga
    52741 tgatcattta aatgaacatg gctatatcgt gccaggcttg ggcgatgctg gtgatcgttt
    52801 atttgggaca aaataaaaga gacaaccatc aaatagtagc ctttttaggc caatcgttct
    52861 tgattttgtt taaaaaatca gagagatttc tcttctttaa aggaaaagca agtttatgat
    52921 ttgaccttta ttgaccaaaa tattataata aagacaatca gtaaaaaaga gatgacctag
    52981 tgggttgccg tctctcaaaa tgacgctact aaaaaccctt gtagattgga gaatgtatta
    53041 tgattcctgt tgttattgaa caaactagcc gtggagaacg ttcttatgac atttattcac
    53101 gtttattaaa agaccgtatt atcatgttaa cagggcctgt cgaagacaac atggccaact
    53161 ctgtcatagc gcagctctta ttccttgacg cgcaagacaa cacgaaagat atctacctat
    53221 atgtcaacac accaggtggc tctgtctcag caggcttggc tattgtcgat accatgaact
    53281 ttatcaaggc cgatgtgcaa actattgtca tgggtatggc agcttctatg gggacagtca
    53341 ttgcttcatc aggaacaaaa ggcaaacgct ttatgttacc aaatgcagag tatatgatcc
    53401 accagccaat gggtggcaca ggcggtggca cgcaacaaac ggatatggct atcgcagcag
    53461 aacatctctt aaaaacacgt caccgtttag aaaaaatctt agcgcaaaat gctggaaaaa
    53521 cgattaaaca aatccataaa gatgctgagc gtgattattg gatgagtgct gaagaaacct
    53581 tggcttacgg tttcattgat gaaatcatgg aaaacaacga actaaaataa agaggatctc
    53641 tgcaaaagta ttctagaaac ttctaggatg ctttttctct tagaaaggaa aataatgata
    53701 cagcctactg cattaaaaca gcttcaagag ttaaaacgtc agttcagatc cctttgcaac
    53761 caattatgag atagtttgag gatgatcctt agcagctgtg tgcaagaagt tatgacgatt
    53821 tcataaagga tgacctagcc agtctttctt cgtctctttt acttaaagtg tggtataatc
    53881 agattatgct agccaaaact aaaaaatgca ttgccattaa aggtgttgta gatcggctta
    53941 tcctagatct cataacaatg ctgtagtacc gcccttacct tgctactaaa ggcgctgttg
    54001 aagcacaaat tgacttgcaa aggatcttga ctgataggta tatgtccggt ggaatcgcta
    54061 aagcagcaaa aagaccaaga cccttctttg gtctctacca aaatataata gaaaaggatt
    54121 tagaatgttt caaaaacaag aacgtattgg actcgtcgtt tacctgtatt ataaccgaga
    54181 tgccagaaag ttatcaaaat ttggtgattt atattaccat tccaagcgct ctcgttacct
    54241 cattatttat attaataaaa atgatttaga cacaaaatta gaagaaatga gacgtttgaa
    54301 atgtgttaaa gacattagac catcagcttt tgatgatatt gaccgccaat ttgtaggtaa
    54361 ccttcatcgc gacgaaacaa acaaccatca aaagggttaa caccccccta gttactgatt
    54421 agcgtttcag atttagttag cttattaaag taagaaggtt ttgacagatg ctcacactac
    54481 ttcaagacaa tgagacaact agagcattag tgaataactg agttgactgt cacttcttta
    54541 ataagaacgg ataggattgg tcctaaaata gagcactgca aagatatgcc agtgcttttt
    54601 agctcatttt ttgataaaat agaactacta aaaagtgaag gaaataagat gatcacagga
    54661 aaactcataa ccgttgaagg tccagatggc gcagggaaaa cgactgtttt agagcaactc
    54721 attccactgt taaaacaaaa agtggctcag gagatcttga caacaagaga gcctggaggt
    54781 gttgccattt cagaatacat tagagaactt attttagaca tcaaccatac tactatggac
    54841 cctaaaacag agctcttact ttatattgcg gctcgaaggc agcatttggt ggaaaaggta
    54901 ctaccagctc ttgaagcagg ccagttggtt tttattgatc gtttcatcga tagttcagta
    54961 gcttatcaag gtgcgggacg cggtttgacc aaagcagaca ttcagtggct caacgagttc
    55021 gcaacagatg gtttagagcc tgacttgacc ctttattttg atgttccttc tgaaattggt
    55081 ttagcacgga ttaacgcgaa ccaacaaaga gaagtcaatc gtttagattt ggaaacaata
    55141 gagatacatc aacgagtacg aaaaggatac ctagccttag ctaaggagca tccaaaacgt
    55201 attgtcacaa ttgatgcgac taaaccattg aaagaagttg tctctgtcgc tttggaacat
    55261 gtcttagcct tgcttttagc ctaggataag ggagggtagg aatggattta gcgcaaaaag
    55321 ctcctaacgt ttatcaagct tttcagacga ttttaaagaa agaccgtctg aatcatgctt
    55381 atcttttttc gggtgatttt gctaatgaag aaatggctct ttttttagct aaggtcatct
    55441 tttgtgaaca gaaaaaggat cagacgccct gcgggcattg tcgctcttgt caattgattg
    55501 aacaaggaga ttttgccgat gtgacggtat tggaaccaac agggcaagtg attaaaacgg
    55561 atgtggtcaa agaaatgatg gctaactttt ctcagacagg atatgaaaac aaacgacaag
    55621 tttttattat caaagattgt gacaaaatgc ataccaatgc cgctaatagc ttgctaaagt
    55681 atattgagga gcctcaggga gaagcttaca tgtttttgct gaccaatgat gataacaaag
    55741 tgcttccgac cattaaaagt cggacacagg tttttcagtt tcctaaaaac gaagcctatc
    55801 tttaccaatt ggcacaagaa aagggattat taaaccatca ggctaagcta gtagccaaac
    55861 ttgccacaaa caccagtcat ctagaacgtc tgttgcaaac gagcaagctt ttagaactga
    55921 taactcaagc agagcgtttt gtatctattt ggctgaaaga tcagttgcag gcatatttag
    55981 cgttgaaccg tctggtacag ttagcaactg aaaaagaaga acaagattta gttttgaccc
    56041 ttttgacctt gctcttggca agagagcgtg cgcaaacgcc tttgacacaa ttggaggctg
    56101 tttatcaggc taggctcatg tggcagagca atgttaattt tcaaaacaca ttagaatata
    56161 tggtgatgtc agaataagtg caattggaat tcgttatggg gaaacgaagc ttgtcaaaag
    56221 acttgccctt agcgaccaaa gtagcggcgc ataggggcaa aacaagcttt tgaagaaaat
    56281 actgcctttg caacagattt gatgaaagaa gtatcacgat taataacaca aaatcaactt
    56341 gaaatgacgt ttattaatag tgtctttccc ttagatcagc atgttgtcct aattcccttt
    56401 tcagcagaag agcacatgga tttttacaac tgttaaaaga tttagcggcc gattttaggt
    56461 caagaagtga gctgccccag attaacagcc aagaagaaac caagatttat gggcgcttag
    56521 gaccgtgtgg cagaccactg tgctgctttc gtttttggga aaatttctga cagtatctat
    56581 taaaatacta aaaaatcaaa ggttatcatt gactttgggt aaagcaacaa gcccttatgg
    56641 ccgtttgctt tgctgtctct atgatgaagg tggcttttac caataaagca agaaaaaatt
    56701 ccctgaatat ggtaccatag tagagacaag tgatggccct gcaacagtag cagccttgat
    56761 atatttacag atcccattaa ggtgcggtta aaagaccagt tgaccttagt cacttatgct
    56821 ttagaggagg ttaaagtagg tgaataaaaa agaattattc gatgcttttg atggattttc
    56881 ccaaaattta atggttactt tagctgaaat tgaagccatg aaaaaacaag tccaatcact
    56941 agtagaagaa aacacgattc ttcgcctaga aaatacaaaa ctacgggaac gtttgagtca
    57001 cctagaacat gaaacggtag ctaaaaatcc ttccaaacaa aggaaggatc acttagaggg
    57061 aatttatgat gaagggtttc atatctgcaa ttttttctat ggacaacgtc gtgaaaatga
    57121 tgaagaatgt atgttttgca gagaattatt agatagaaag taagggcatg caggttcaaa
    57181 aaagttttaa agacaaaaaa acaagcggaa ccttgtattt ggttccaacc ccgattggca
    57241 atctgcagga catgaccttt agagcagtgg caacgttaaa agaggttgat tttatttgtg
    57301 cagaagacac aagaaacaca ggtcttttgc taaaacactt tgacattgct accaagcaaa
    57361 tcagctttca tgagcacaac gcctatgaga agattcctga tttaattgat ttattaatat
    57421 caggacggtc attagctcag gtgtctgatg caggaatgcc atcgatttct gatcctgggc
    57481 atgatttggt caaggctgct attgatagtg atatcgcagt cgttgcttta ccaggtgcct
    57541 cagctgggat tacagctttg attgctagtg gactggctcc tcaaccccat gttttttatg
    57601 ggtttttacc cagaaaagca gggcaacaaa aggctttctt tgaggacaaa caccattacc
    57661 cagagacaca aatgttttat gaatcaccct atcgcatcaa agataccctg accaatatgt
    57721 tggcttgtta tggtgatcgt caagtggtgc ttgtcagaga attaaccaag ctttttgagg
    57781 agtaccaacg gggggcgatt tcagaaatct tgtcctacct tgaggaaaca cccctaaaag
    57841 gggagtgtct gctgattgtg gctggagccc aagtagacag tgaggttgag ctgacagctg
    57901 atgttgactt agtgtctcta gtgcaaaaag aaatccaagc aggagccaag ccaaatcagg
    57961 ctattaaaac aattgctaaa gcttaccagg taaatcgcca agagctttac caacaatttc
    58021 atgacttata agtcatcgta gattaggaga aaaacaaatg acagaagatc aagcaactat
    58081 ccttatttta ggagtatacg gaatcatatt ggctgtattc ttattatcag ctttattatg
    58141 ccttatcgct aactggcgac tctttaaaag agcaggttac aaaggatggt atagtctcat
    58201 tcctttctat gcaggttttg tcaaacacaa aatcactttt ggagaagata ataaatggtt
    58261 ttactttatt ggttgggtca ttgctattta ttattactat gtgcactttt gctatgtccg
    58321 tgcttttggt ggatcaaagg gtctagcagt atttggcttg tttttcccag gcattgcttc
    58381 tttgatagtg gcttttggaa aaacttatac cacacaagaa tgccaccctg tgccacatct
    58441 tttaaactaa gagagaaaag actatcaatt ggatttattg atggtctttt aggtgtttaa
    58501 atgattttag tcccgtgaac aatggccgtt ccagtgagtt gactcaagct ttggcaattc
    58561 tcagcagtga cgcctcctcc aatcatgatt tcaatgcgtt tattggcata ggtcacaaga
    58621 cttttaagtt gttccacatt gtctgtaata ggagtagctt caggggagcc atgagttagc
    58681 acacgcacaa agccataatc aatcagttga tcaagcgctt ggtgctggtg atcagtcgga
    58741 atatggtcaa aagccatgtg gaaaaccagt ggcaagcctt gtgtagcagg cagtaattgc
    58801 tcgatagcgt cggtatccag ttgattttct gtggtcagca agcctaagac tagcgcatct
    58861 gagccagctt caacagcttt taagatatct tcttccatag cttttagttc gaggtcgtta
    58921 tagacaaaat cgccaccgcg aggtcgaatc atggtcgcta cgctaatctt tttatcgtgt
    58981 aagagctgac aggcttcttt gatgacgcca taagatggcg tggtccctcc gacagctagg
    59041 ttatcacaga gttccacacg actgacttga ccagcatcaa gggttggcag tagtgtgagg
    59101 ttctcagcac aaaattcttt aatcattttt agttctcctt tatcactaat attatagcac
    59161 gaatgaagct agtcatcagg aaacattttt gtcaaatcat ggacattatg ctatactcac
    59221 tagtattaaa agtaggccaa aacagcagca aatactttga aaaaagcttg ctatgttcta
    59281 cggcaatatc agtctttacc aacgaactct caagaagaaa agaaagacaa agcttagcat
    59341 tttaaaaaag cctccccttt ttttgtgaaa tgatgaccaa aaacaggcgc ctcacgccta
    59401 aaacagttac cctaaaggag aaaaaatgat tacgatttac caatacccaa aatgtagcac
    59461 ctgccaaaga gccatggctg aattaaagca actggttagt gactttgaag ccattgacat
    59521 caaagctaat ccacccaaag ctcaggacct caaacattgg atggaaacat ctggttatac
    59581 catcaaaaat ttctttaata ccagtggtaa cagctaccgc gaattgggac ttaaagataa
    59641 aatagaccag cttagccttg ataaagctgc tgaactctta gccacagatg gtatgctgat
    59701 taaacggcct attctgatta aggacggtaa agtcctacaa gttggctatc gcaagccata
    59761 tcaagagttg gatttaggat aatgcgacaa agtctcaaaa aaggacaagg caacattata
    59821 acgccttgtc cttttttggg atctgttttt agacattaga gatcaatatc tagtaaaatt
    59881 ggtgtatggt cttggcgttc cccagaagaa atcatttcag aacgcttaac cttatccacg
    59941 attcggtttg aagctaggca atagtcaatt ctccaaccgg tattattgat tttgctggtt
    60001 ttgctacgtt gtgcccacca ggtataaaca tgaggaatat cgccgtgcaa gtgacgaaaa
    60061 gtatctgtaa agcctcttgc taataggtta gtaaagcctt gacgttcttc gtcggtaaaa
    60121 ccaggggaac gtcgattgct gcttggattt gccaaatcaa tctctttgtg ggcaacgttg
    60181 taatctcccg tagccaaaac aggcttttga gtatcaagtt cagtcaagta atcagcgtat
    60241 ttgtggtccc agatttgacg gtcatctaac cgtcttagac catcaccagc attaggcgtg
    60301 taaacctgtg tgacgaaaaa gtcttcaaat tctagggtaa tgatacggcc ctcagcatcc
    60361 attgttgttg gggcaccaat ttctggaaaa gtaatgacag ggtttaacgt gtttttgtac
    60421 aggaacatgg tgccagcata gcctttccta gcaggctcaa ctgaagaacg ccacacatgc
    60481 aaatagtttg gaaagtaact aagtagggtc tcaatatgtt ttttggtagg ccccttagca
    60541 gataatttgg tttcttgaat ggcaataata tccgcatctt gagcgactaa agtgtctaaa
    60601 acagcgcgtg ataaaagcgc acgaggggac tctcctgtga gagcagcatt taaggaatca
    60661 atgttccatg aaatcagttt catttggttt ctctttctaa cagatagtct tgttgttatt
    60721 gtaacaaaaa acgaaacgat aagcgacttt gggcacctag cccccataaa acagtctagc
    60781 caaatgaagc atagcagcgt aagaacaaga caaggaggtg atcagtgagc atatcgtttg
    60841 acagttaaga gtaggattac tataatagaa acacaaatgt aagcgctaac aaaataaaaa
    60901 atataggaga atgtcaatgg cacaaaaaac agtcatcacc gaagaaacaa ccgattttgt
    60961 tatggatttc aaaacaagta gcgctgaggg aaatgttgat tttattaacg tttttgacct
    61021 tgagaaaatg gcacaacagg tcatccctaa aggagccttt ggttatattg ctagtggtgc
    61081 tggtgatacc tttaccctcc atgaaaatat tcgctcattt aatcataaat taattgtccc
    61141 tcactccctc aaaggggttg aaaatccgtc aacagagatt acgtttgatg gtgattactt
    61201 aacctctcct cttattttag cacctgtagc ggctcataaa ctggccaatg aacaaggaga
    61261 agtagcgtct gctaaaggat tgaaggaatt tggaagtatc tacaccacta gttcctactc
    61321 aacaactgac ctgccagaaa tcagtgctgc cttaggtggg acaccccatt ggtttcaatt
    61381 ttattacagt aaagacgatg gcattaatcg taatattatg gatcgtgtca aagcacaagg
    61441 ttgcaaagct atcgttttga cagcagatgc aacagttgga ggaaaccgcg aagttgatag
    61501 gcgaaacggt tttgtttttc cggtcggtat gccaattgtt caagaatatt tacctgatgg
    61561 tgcaggtaaa acaatggatt atgtctataa atcagctaaa caagcgctta cctcaaaaga
    61621 tattgaatat atcgcaactt actcaggcct gcctgtttat gtcaaaggac cacagtgtgc
    61681 tgaggatacc ttgcgggcat tggacgcagg agcatcaggt atctgggtaa ccaatcatgg
    61741 tggccgtcaa ttagatggtg gtccagctgc ttttgattcc ttacaagaag tcgctgaagc
    61801 agttaatcaa aaagttccta ttgtctttga ctcaggtata agacgaggtc aacatatttt
    61861 caaagctctt gcttcaggtg ctgaccttgt tgccttgggg cgtccagcga tttacggctt
    61921 ggctatggga ggaagcattg gtactcgtca ggtctttgaa aagttaaatg acgagttaaa
    61981 aatggtcatg caactggctg ggacacaaac tatccaagat gttaaagcct ttaaccttcg
    62041 ccacaatccg tatgattcat cgattccatt tgatcaaaat gcgttacgct tagattagaa
    62101 cagacaaaaa cctccgctat catgtcacaa atagtagagg ttttacactg caaaaagagg
    62161 tatgaaaatc caataacata gttacaaggc tttcgcttac caacccctac taatagatta
    62221 aaatgaaaga cttcttacat taatagaaat ttctgttgct tttctcagaa aaatgtggtt
    62281 aaataatcaa atattattaa cgaaaaatga gttaatgatt aacattaaac tggttttagt
    62341 taaaacattt aggagggtat caggtggaga aaaagcaacg tttttccctt agaaaataca
    62401 aatcaggaac gttttcggtc ttagtaggaa gcgttttctt gatgatgaca acaacagtag
    62461 cagcagatga gctaaccaca acgagtgaac caacaatcac gaatcacgct caacaacaag
    62521 cgcaacctct caccaataca gagttgagct cagctgaatc acaatcccca gacacatcac
    62581 aagtaactcc agagacaaat cgtgaaaaag agcaatcaca agatctagtc tctaagccaa
    62641 ccacaactga gctagctgac acagatgcag caccaatggc taatacaggt cctgatgcga
    62701 ctcaaaaaag cgcttcttta ccgccagtca atacagatgt tcacgattgg gtaaaaacca
    62761 aaggagcttg ggacaaggga tacaaaggac aaggcaaggt tgtcgcagtt attgacacag
    62821 ggatcgatcc ggcccatcaa agcatgcgca tcagtgatgt atcaactgct aaagtaaaat
    62881 caaaagaaga catgctagca cgccaaaaag ccgccggtat taattatggg agttggataa
    62941 atgataaagt tgtttttgca cataattatg tggaaaatag cgataatatc aaagaaaatc
    63001 aattcgggga ttttgatgag gactgggaaa actttgagtt tgatgcagat gcagagccaa
    63061 aagccatcaa aaaacacaag atctatcgtc cccaatcaac ccaggcaccg aaagaaactg
    63121 ttatcaaaac agaagaaaca gatggttcac atgatattga ctggacacaa acagacgatg
    63181 agaccaaata cgagtcacac ggtatgcatg tgacaggtat tgtagccggt aatagcaaag
    63241 aagccgctgc tactggagaa cgctttttag gaattgcacc agaggcccaa gtcatgttca
    63301 tgcgtgtttt tgccaacgac gtcatgggat cagctgaatc actctttatc aaagctatcg
    63361 aagatgccgt ggccttagga gcagatgtga tcaacctgag tcttggaacc gctaatgggg
    63421 cacagcttag tggcagcaag cctctaatgg aagcaattga aaaagctaaa aaagccggtg
    63481 tatcagttgt tgtagcagca ggaaatgagc gcgtctatgg atctgaccat gatgatccat
    63541 tggcaacaaa tccagactat ggtttggtcg gttctccctc aacaggtcga acaccaacat
    63601 cagtggcagc tataaacagt aagtgggtga ttcaacgtct aatgacggtc aaagaattgg
    63661 aaaaccgtgc cgatttaaac catggtaaag ccatctattc agagtctgtc gactttaaag
    63721 acataaaaga tagcctaggt tatgataaat cgcatcaatt tgcttatgtc aaagagtcaa
    63781 ctgatgcggg ttataacgca caagacgtta aaggtaaaat tgctttaatt gaacgtgatc
    63841 ccaataaaac ctatgacgaa atgattgctt tggctaagaa acatggagcc ctgggagtac
    63901 ttatttttaa taacaagcct ggtcaatcaa accgctcaat gcgtctaaca gctaatggga
    63961 tggggatacc atctgctttc atatcgcacg aatttggtaa ggccatgtcc caattaaatg
    64021 gcaatggtac aggaagttta gagtttgaca gtgtggtctc aaaagcaccg agtcaaaaag
    64081 gcaatgaaat gaatcatttt tcaaattggg gcctaacttc tgatggctat ttaaaacctg
    64141 acattactgc accaggtggc gatatctact ctacctataa cgataaccac tatggtagcc
    64201 aaacaggaac aagtatggcc tctcctcaga ttgctggcgc cagccttttg gtcaaacaat
    64261 acctagaaaa gactcagcca aacttgccaa aagaaaaaat tgctgatatc gttaagaacc
    64321 tattgatgag caatgctcaa attcatgtta atccagagac aaaaacgacc acctcaccgc
    64381 gtcagcaagg ggcaggatta cttaatattg acggagctgt cactagcgga ctttatgtga
    64441 caggaaaaga caactatggc agtatatcat taggcaacat cacagatacg atgacgtttg
    64501 atgtgactgt tcacaaccta agcaataaag acaaaacatt acgttatgac acagaattgc
    64561 taacagatca tgtagaccca caaaagggcc gcttcacttt gacttctcgc tccttaaaaa
    64621 cgtaccaagg aggagaagtt acagtcccag ctaatggaaa agtgactgta agggttacca
    64681 tggatgtctc acagttcaca aaagagctaa caaaacagat gccaaatggt tactatctag
    64741 aaggttttgt ccgctttaga gatagtcaag atgaccaact aaatagagta aacattcctt
    64801 ttgttggttt taaaggacaa tttgaaaact tagcagttgc agaagagtcc atttacaggt
    64861 taaaatctca aggcaaaacc ggtttttact ttgatgaatc aggtccaaaa gacgatatct
    64921 atgtcggtaa acactttaca ggactcgtca ctcttggttc agagaccaat gtgtcaacca
    64981 aaacgatttc tgacaatggt ctacacacac ttggtacctt taaaaatgca gatggcaaat
    65041 ttatcttaga aaaaaatgcc caaggaaacc ctgtcttagc catttctcca aatggtgata
    65101 acaaccaaga ttttgcagcc ttcaaaggtg ttttcttgag aaaatatcaa ggcttaaaag
    65161 caagtgtcta ccatgctagt gacaaggaac acaaaaatcc actatgggtc agcccagaaa
    65221 gctttaaagg agataaaaac tttaatagtg acattagatt tgcaaaatcg acgaccctgt
    65281 taggcacagc gttttctgga aaatcgttaa caggagctga attaccagat gggtattatc
    65341 attatgtggt gtcttattac ccagatgtgg tcggtgccaa acgtcaagaa atgacatttg
    65401 acatgatttt agaccgacaa aaaccggtac tatcacaagc aacatttgat cctgaaacaa
    65461 accgattcaa accagaaccc ctaaaagacc gtggattagc tggtgttcgt aaagacagtg
    65521 tcttttatct agaaagaaaa gacaacaagc cttatacagt tacgataaac gatagctaca
    65581 aatatgtctc agtagcagac aataaaacat ttgtggagcg acaagctgat ggcagcttta
    65641 tcttgccgct tgataaagca aaattagggg atttctatta catggtcgag gattttgcag
    65701 ggaacgtggc catcgctaag ttaggagatc acctaccaca aacattaggt aaaacaccaa
    65761 ttaaacttaa gcttacagac ggtaattatc agaccaaaga aacgcttaaa gataatcttg
    65821 aaatgacaca gtctgacaca ggcctagtca caaatcaagc ccagctagca gtggtgcacc
    65881 gcaatcagcc gcaaagccag ctaacaaaga tgaatcagga tttctttatc tcaccaaacg
    65941 aagatgggaa taaagacttc gtggccttta aaggcttgaa aaataacgtg tataatgact
    66001 taacggttaa cgtatacgct aaagatgacc accaaaaaca aacccctatc tggtctagtc
    66061 aagcaggcgc tagtgcatcc gctattgaaa gtacagcttg gtatggcata acagcccgag
    66121 gaagcaaggt gatgccaggt gattatcagt atgttgtgac ctatcgtgac gaacatggta
    66181 aagaacatca aaagcagtac accatatctg tgaatgacaa aaaaccaatg atcactcagg
    66241 gacgttttga taccattaat ggcgttgacc actttactcc tgacaagaca aaagcccttg
    66301 gctcatcagg cattgtccgc gaagaagtct tttacttggc caagaaaaat ggccgtaaat
    66361 ttgatgtgac agaaggtaaa gatggtatca cagttagtga caataaggtg tatatcccta
    66421 aaaatccaga tggttcttac accatttcaa aaagagatgg tgtcacactg tcagattatt
    66481 actaccttgt cgaagataga gctggtaatg tgtcttttgc taccttgcgt gacctaaaag
    66541 cggtcggaaa agacaaagca gtagtcaact ttggattaga cttaccggtc cctgaagaca
    66601 aacaaatagt gaactttacc taccttgtgc gggatgcaga tggtaaaccg attgaaaacc
    66661 tagagtatta taataactca ggtaacagtc ttatcttgcc atatggcaaa tacacggtcg
    66721 aattgttgac ctatgacacc aatgcagcca aactagagtc agataaaatc gtttccttta
    66781 ccttatcagc tgataataac ttccaacaag ttacctttaa gatgacgatg ttagcaactt
    66841 ctcaaataac tgcccacttt gatcatcttt tgccagaagg cagtcgcgtt agccttaaaa
    66901 cagctcaagg tcagctgatc ccgcttggac agtccttgta tgtgcctaaa gcttatggca
    66961 aaaccgttca agaaggtact tacgaagttg ttgtcagcct gcctaaaggc taccgtatcg
    67021 aaggcaacac aaaggtgaat gccctaccaa atgaagtgca cgaactatca ttacgccttg
    67081 tcaaagtagg agatgcctca gattcaactg gtgatcataa ggttatgtca aaaaataatt
    67141 cacaggcttt gacagcctct gccacaccaa ccaagacaac gacctcagca acagcaaaag
    67201 ccctaccatc agcgggtgaa aaaatgggtc tcaagttgcg catagtaggt cttgtgttac
    67261 tcggacttac ttgcgtcttt agccgaaaaa aatcaaccaa agattgataa caaagcgcaa
    67321 agagacaaca aatcatccct tttaacagtc tgacctttct ttgagattga gctggcagat
    67381 gatcatttct taacaaaagt taaagagttg agcaaagtaa ggaacagaca taaagaccat
    67441 aaaagcaagc cagtttggct tgcttttatg gtgttattgt cgtgtgctac gatgactgaa
    67501 aaagagcaac gaacaatgac ctcgaatcac caaaaggact tgatttgata gcttccctat
    67561 gctatactaa gcctattctt tcaagaagga ctccttttgt ggaaaagact gaaaaaaagg
    67621 acactaatct atccaaaaac tccagatgag tagctgcatg agtagctgca accggatgcc
    67681 tctgctgtga tcaaggtggt aaagcagact ggcttgtcac tacctgagca ggtgtcaaaa
    67741 aaagaagctt tggctttgtc attcaacata ctgagcttta gtctttcaga acggagacac
    67801 ccccagtttt gtactcaaaa agcataaaca gctctgctga tgcgacatcc gatttctacg
    67861 tccgcagtga gccaaaccag ctgctgtttg ctatggtaaa agtggcgcca atcgggattt
    67921 ttgagatcct aggctcaaaa ataaggcata gaattccaaa ggaagtcgct gccgtgagca
    67981 caacctaaga aaaactattc caaaagactt tttctgcaat ttgaaatact gaatttcagt
    68041 attttttctt ttgtaactaa gagcaatgaa tgaggtaaaa aaaggcaaca atgtcaaaaa
    68101 agaaacgaaa acactatcag gtttatgagg gattacgctg tgctatgacg ctttgtttta
    68161 tcagtggcta tgtcaatgcc tttacctata tgacacaagg caaacgtttt gcaggtgttc
    68221 agacaggcaa ccttttgtca tttgccattc gcttgtcaga acagcaactg aaggaggcac
    68281 tgcaattttt acttcccatg atagttttta tgcttgggca gtcctttact tatttcatgc
    68341 accgctgggc cactaaaaaa gggctccact ggtacttatt atcaagcttt atcttaacag
    68401 ggattgcttt tggaacggcc ctattcacgc cttttcttcc ttccaatgtt acggtagcag
    68461 ctttagcctt ttttgcctct attcaagttg atacgtttaa aaccttacga ggagctagct
    68521 atgctaatgt gatgatgaca ggaaatatca aaaatgccgc ctatttacta accaaaggct
    68581 tgtacgaaaa aaatcacgaa ctaacacata tcggacgcaa caccttaata gtcattttgg
    68641 cttttgctgt tggggtggtt tgttcgacgt tgctttgcat agcttatggc gagtatgctt
    68701 tgatacctat tttaatgcct ttactctatg tcaattacct gttggctcag gaattttatc
    68761 acattcaaac caaaatcaaa ccgattaaaa gaacttaacg gacttgtgga gaaagaaagt
    68821 tcatttaggg taagttttta ctgaccctcc gttgatttgc tggttttgct gccccttaaa
    68881 atatgatata atagaccatt gatagctgtt acttaacaga aggctgataa aaaaggttcc
    68941 attagccttg agggttgaat ttaaacaggc aaaaacgaaa tcctacacta tgcatctcaa
    69001 tcatactttc atttgagtat ggttctgaaa ggaaactatg atgaaaaaac cattttacat
    69061 tacgacaccc atttattacc catctgggaa attacacatt ggttctgcct atacaactat
    69121 tgcttgtgat gtcttggcac gctacaagcg cctgatgggc catgaggtct tttacctcac
    69181 aggactagat gaacacggac aaaagattca aaccaaggca aaagaagctg gtattacacc
    69241 tcaaacctac gttgataaca tggcaaagga tgtcaaagcc ctttggcaat tgctagatat
    69301 ttcctatgac acatttatcc ggacaactga cgattaccac gaagaagtcg ttgctgctgt
    69361 ttttgaaaaa ttgctggcgc aagacgacat ttaccttggc gaatactctg gttggtattc
    69421 tgtctctgat gaggaatttt tcactgaaag ccaattaaaa gaagtcttta gagatgaaga
    69481 cggtcaggtt attggaggga ttgccccatc aggtcatgag gtcgagtggg tctctgaaga
    69541 atcctatttc ctacgcctta gcaaatacgc tgattgtctt gtggccttct ttaaagagcg
    69601 acctgacttt atccaaccag atggtcgtat gaatgaaatg gtgaaaaact ttatcgagcc
    69661 aggtctagaa gatttggctg ttagccgtac tacctttaca tggggtgtac ctgttccatc
    69721 agacccaaaa cacgtggttt atgtgtggat tgatgccctc ttaaattacg cgacagctct
    69781 tggttatggt caagctaatc acgctaactt tgataaattc tggaatggaa cggttttcca
    69841 catggttgga aaagatatct tacgttttca tagcatctac tggccgatct tactcatgat
    69901 gcttgattta ccaatgccag accgcttaat tgctcatggt tggtttgtta tgaaagacgg
    69961 taaaatgtct aaatcaaaag gcaatgtggt ctatccagaa atgttagtgg agcgctttgg
    70021 acttgatcct cttcgttatt acctcatgcg ttctttgcca gtgggttcag acgggacctt
    70081 tactcctgaa gattatgtgg gtcgtatcaa ctatgagtta gccaacgacc ttggtaattt
    70141 attgaatcgt accgttgcga tgatcaacaa gtattttgat ggcacggtgc cagcttacgt
    70201 tgacaacgga actgcctttg atgctgactt aagtcaactg attgatgctc agctagcaga
    70261 ctatcataag tacatggaag cggtggatta cccacgcgcc ttggaggctg tttggaccat
    70321 catcgctcgt accaacaaat acatcgatga aaccgctcct tgggtgctcg ctaaagaaga
    70381 tggtgacaaa gctcaactag ccagtgtcat ggcacacttg gcagctagct tgcgcgtggt
    70441 cgctcatgtg atccaaccct ttatgatgga aacatcagct gctatcatgg cccagcttgg
    70501 cttagcgcct gtttctgact tgtcaacact tgctctagca gcttttcctg ccaatacaaa
    70561 ggtcgttgcc aaaggcacac cgattttccc acgtcttgac atggaagctg agattgacta
    70621 catcaaagct caaatgggtg acagctcagc tatttcccaa gaaaaagaat gggtccctga
    70681 agaggttgcg ctcaaatctg aaaaagacgt catcacattt gaaacctttg atgctgtgga
    70741 aatccgggtg gcagaagtca aggaagtgtc aaaagtggaa ggttcagaaa aattgctgcg
    70801 ctttagagta gatgcaggag acgggcaaga ccgtcaaatc ttgtcaggta ttgccaaatt
    70861 ttaccccaac gaacaagaat tagtgggcaa aaaactacaa attgttgcta accttaaacc
    70921 tcgtaaaatg atgaaaaaat acatcagcca aggcatgatt ttatcagcag agcatggaga
    70981 tcagctaacg gtcttaaccg ttgattcctc tgttccaaat ggaagtatca ttggttgatg
    71041 ctgacctatt attcaaaaaa cctaagcaag aatatgtgct taggtttttt tgattgcctt
    71101 taagaaagac gaataacgag atgggtgata tggcatcaaa agcataacta tcctaggacc
    71161 tgtcctagta agattgctgt gcttttgaca agtgaacaga gaggtcttaa actatatctt
    71221 gtcataaaaa agattgcaaa acactatatc ttgtgttatt atggttttta taaaagttaa
    71281 gcatgcttaa ctttagccaa acaaaagagg acactagagc gtgtcccaca agacagttga
    71341 gcccttcgct tttttagtag agtaggagtg ctcaagtcaa aaagagacat cccatcaaac
    71401 cattgctgtt tagcaggtct ttttgtgttg ctcaaaaaga caattcctgt ctgtttggcg
    71461 tctagtgatg acttaggctt agcgctacta gacttttgtt tgaaaaatac ctcaagcctt
    71521 gaaggagaca aatgacccaa cattattatg aacgttcgca atccccaatt gaatacgccc
    71581 tatctgaaac acaaaaacag ttgcgctcca tcaattggaa ttaccttaat gatgacaaag
    71641 atttggaagt gtggaaccgt gtcactcaaa atttttggtt gcctgaaaag gttcctgttt
    71701 ctaatgattt aaattcttgg cgctccttgg gtgaggattg gcaacagctc atcacaagga
    71761 cctacactgg gttgacttta ctagatacag tacaagcaac ggttggtgat gtggcacaaa
    71821 ttcagcattc acaaaccgac cacgaacaag tcatctatac gaactttgcc tttatggtgg
    71881 ggatccatgc ccgctcttac gggacgattt tttcgaccct ttgcagtagc gagcaaatcg
    71941 aagaagcaca tgaatgggtg gtctcaacac agagcttgca agaccgtgcc cgtgtcttga
    72001 ttccttacta tacgggtgat gaccctttga aatcaaaagt agcagcagct atgatgccag
    72061 gatttttgct gtatggcggc ttttaccttc ctttttacct atcagcacgt ggtaaaatgc
    72121 caaacacatc tgatatcatc cgcctgattt tacgcgacaa agttattcac aactattaca
    72181 gcggttacaa ataccaacaa aaagtagcac gtcttagccc agaaaaacag gctgaaatga
    72241 aggcctttgt ctttgacttg ctctatgaac tcatcgacct tgaaaaagct tacttgagag
    72301 aactgtatgc aggctttgat ttggcagaag atgctatccg ctttagcctt tataacgctg
    72361 gaaaattcct tcaaaattta ggctatgatt cgcccttcac agaagaagag acccgtatct
    72421 cacctgaagt atttgctcag ctatcagcgc gtgcagacga aaaccatgac tttttctcag
    72481 gcaatggctc atcttatgtg atggggatca ccgaagaaac cacagatgac gattgggagt
    72541 tttaagaaat ggcagagctg attattgtgt atttttcatc taagtccaat aacacccatc
    72601 gcttcgtcca aaaattaggc ttgcctgctc agcgcatacc agtagataat aggccacttg
    72661 aagtttcaac ccattacctt ttgattgtgc ctacctatgc agctggtggc tcagatgcca
    72721 aaggagctgt ccctaaacaa gtgattcgtt ttttgaataa tcctaacaat cgtaaacatt
    72781 gtaagggagt gatctcttca ggaaatacca attttggcga tacctttgcc ttagcaggcc
    72841 ctatcatttc tcaaaaattg caggtcccct tattacacca atttgaacta ttaggcactg
    72901 ctacagatgt caaaaaggta caggcgattt ttgcccgctt aaagcatcac acacacgaca
    72961 agcaaaaaca aaccaacaac ctgattacag aaaggacaca cccatgtcac aaaccaatgc
    73021 gtcatacctc tcattaaatg cactgactcg ttttaaaaaa ccagacggca gctaccattt
    73081 tgatagcgat aaagaagctg ttaggcgcta cctggaagaa catgtctctc ccaatcaaat
    73141 ggcttttaac tcactagaag ataagctagc ttacctcatc aatgaagggt attatgagca
    73201 ggccattttt gatgcctacc ctaacgacct tatcaaggag gcctttcatt atgcctatca
    73261 acaaggctat cgctttttaa atctcatggg cgctatgaaa ttttaccagt cctatgcctt
    73321 aaaaaccttg gatggtaagc agtatctaga aacatttgaa gaccgtgctg tgatgaatgc
    73381 actcttttta gctgatggag atcaaacctt tgtctttgac gtgattgatg ccatcttgca
    73441 ccgacgcttt caaccagcta ccccaacgtt tttaaatgcc ggaaaaaaac gccgtggcga
    73501 atacatttcg tgctatcttc ttcgtgtcga agacaatatg gaatctatct ctcgcgccat
    73561 tagcaccagc ttgcaacttt caaaaagagg aggcggagtc gccttgtgtc tcaccaacct
    73621 cagagaaatc ggagcaccga taaaagggat tgaaaatcag gcaacaggca ttgttcctgt
    73681 catgaaacta ctagaggact ctttttccta tgccaatcaa ctaggtcagc gccaaggagc
    73741 aggtgcggtt taccttcatg cccatcaccc cgaggtcctg acctttttag acaccaaacg
    73801 tgaaaatgcc gatgagaaaa ttcgcattaa gtccctagca ttgggacttg tgatcccaga
    73861 tattaccttc cagttagcca aagaaaacaa agacatggca ctcttttcac cctatgatat
    73921 caagcgtgcc tatggcaagg acatgtcaga catctccatc acagaggaat acgataaact
    73981 gctagtcaat ccagccatca aaaagaccta catcagtgct agaaagttct ttcagttcat
    74041 tgcagagcta cattttgagt caggctaccc ttacctcttg tttgatgaca cggtcaataa
    74101 gcgcaaccct catgccaaaa aaggcagaat cgtcatgtct aacctgtgtt ctgaaatcgc
    74161 tcaagtcagc accccaagca ctttcaaaga agatttgagt tttgaaacca ttggagaaga
    74221 tatctgctgt aatttagggt ctatcaatat tgcccaggct atggcagatg cgccacactt
    74281 tgaacagctc atcacaacga gcatcagagc acttgatcgt gtctctagag tgtctgacct
    74341 gaattgtgcg ccttctgttg aggcaggcaa tgcagccaac catgcagtag gattaggcgc
    74401 catgaaccta catgggtttt tggcaaccaa ccacatctac tatgatacaa aagaagccgt
    74461 tgactttaca gatctgtttt tccatgccat ggcctattat gcttttaaag cctcttgcca
    74521 attagccaaa gaaaaaggcg cttttgcagg attttcgtta tcaacctatg cagatggcac
    74581 ttattttgcc aagtatttgc aagaagatgc caagccacaa acagccaagg ttgccacatt
    74641 gttgcaagat tacggcttca cattgccaac ggtggcggat tggcaagccc tagtagcaga
    74701 tatcaagcag tttggcctag ccaatgcaca cttgctagcg gttgctccga caggctccat
    74761 cagctactta tcatcctgca ctccgagtct acaaccagtc gttgctcctg ttgaggtccg
    74821 aaaagaaggt agcctgggca ggatttacgt ccctgcttat cagattgacc aggctaacta
    74881 tgcttactat gaacgtggtg cctatgaggt cggcccaaaa gccatcatcg acgttgtcgc
    74941 tgctgcccaa aagcacgttg accaagccat ctcattgacc ctgtttatga ccgataaagc
    75001 gaccacacgt gaccttaacc gctcctatat ccaagctttt aaacaaaact gtgcatcgat
    75061 ctattatgtg cgagtgaggc aagacgtcct agcaggcagc gaacagtatg acgaggatag
    75121 tcttgtgaca gcttctggag ctagtgatga gaccaccaca gagtgtcaaa gctgtatgat
    75181 ctaacagcta ctagaaagct ctcgtgtccc ctatcacatg cataggatca gtgcgcacga
    75241 cctttcaaga caagcaagca tcagctcttg cttgtctttt tttggcctca aagcccgtta
    75301 gtctgctgct atgcgaggct tttttttgag catcagaacc tcaaaaaaaa ggacatggag
    75361 tccttttttg gtgatcggtg ttgaggccgt cacaaactgc ccttgaagca cgcttctatg
    75421 tggagctttt tgtggtcctg tgacacgtaa gctccaacga cctccagctg acagcctctt
    75481 ggaaacaaga gctcaacctc tgaaggcaca gccgaataag gctcgacaaa ggctgccttg
    75541 gccccttttt tgacacagat gcgcacctcc accggtcgat gggtcatggc gccgtttttc
    75601 aagactgtcg tgctcataaa actgtgcttg gtgtagcgtg taccaagctt gatcttacaa
    75661 aggatatgag ggtcaaactg atggttacga tagtaagacg tgatatcagt gtgtgaaaca
    75721 ccgatatcac gcaaaaacgt ctcgtataca tagcggtata ctacaatgtt ccacgggatg
    75781 actagccggt gagtcgcagc atcgagctgg gccacctgat cccttagctc aggcgtgagt
    75841 tggctgagct cacctttggc cttgtctagg ctggtgttga tagggcctgc ctgctgacag
    75901 gtataagcag ccatcgcctc ttgctcagcc cttgggtaga tgagaccctt gccccagcgc
    75961 tttttaatca gtgtctcagc gcccttttga tccgaaaagg taaaataagc aggcgtcccc
    76021 tcacaaaccg cccgctcata gtgctcacag acaaaaagct cgaccaagcc ctcagtttgc
    76081 cagatcagac taaaacagct aagaagaagg acaatagcca gttgatagcg cttttttagc
    76141 atagttcccc ctgacaccag cccaaaaggc tgacccttcc cctaggcctt ttggctagct
    76201 catgatacca ccagtttctc agacaggcta atctttttgt catctctttc tccctaaaca
    76261 gactctctta tcatgatatt atgctattat gagcgatttg tcaatccata gcaatcgttt
    76321 tttagttaga taagtaagct tttgacctag aggcagacaa gagtggcagt acctcccgtt
    76381 cactgtggtg gctaataagg actaacacaa aatctcaaac gctttcaaaa acagctcctg
    76441 agcttaaaaa aggctgatga acaatttttt tgagtttaag agagattgcc atgattttct
    76501 aatcatttga tgtttaaaat gataaaaaca tgaacttgaa atgaaagaat acgagtagta
    76561 aactataact atatcaaaat gatggaatgg agtgatttta tgaaaacaaa atcaaaacgc
    76621 tttttaaaac tagcaaccct ttgcttggcc ctactaggaa caactttgct aatgacacgt
    76681 cctgtaaagg cggaggtggt gatgacccag agcctagctg agggtagtaa tagttcagaa
    76741 aaaaatagtg agcagcagga ccaggataaa gtacagcaaa gaggtgatag taaagaagat
    76801 tgggatagag ggtatgatga tggttacaag aagggtgaag aatcagatac tcgagaggaa
    76861 ttagatagaa gtacttttga cagttttccc ccaggtatat cggatcaagg agagtatttg
    76921 gatggctatg aagggggata tgaagcagga tggcgtaaag gtcatccagt agaagcattc
    76981 ctggagatgg catggcactt cttaacatat gcatttacta gtttatttgg taaccacggt
    77041 tcttagtaat tgatctattc gttttttaat ttagttatta atgataaaaa aactagaggt
    77101 gtatattaaa cgtatcaaat aataacgaaa tggagtgatt ttatgaaaac aaaataaaat
    77161 tagcaacctt ttgcttggtc cttttgggat caaccttact agtgacatac ccagtaaaag
    77221 cgacagaaac gccattaagt ggatatgagt ctactgaaca agctctacct aaatccaatc
    77281 aagtaacgga gaccttacat agtgaagggg atttacaaaa aattgcagaa aaaagcgacg
    77341 atgggtataa gcttgggtat caaaaggggt atgaagctgg tcgaaaagaa gatgcttccg
    77401 aaaatccaga cgaaaataaa ttagaatcta agtctcttga ttatattagt ggttattacg
    77461 caggatatta tcgagggtgg gatgaaacta acaaaccttt ccaaacatta ttagaagata
    77521 tttatcgttg gttatgggaa attttcttta aaccgtaagt aaatgttaat tattcattaa
    77581 aatatagtca taaaatgtga taacatctat ttattatagt tattttactt gaggaagata
    77641 gtaccttgta tgcatgcaga agcatattat aaagatctgc tataattggg atagtccctt
    77701 tagtagtcag gccacctctg ataggggtgg cttttttggg ttttagtgac ccgtgtgttg
    77761 agaaaggagc atttatgcca gaaaagaaag tgttgcccgt ccgttgccta atatgaaccg
    77821 acagttattc cctattaaaa tagacgcaaa tatccacgct ttcactgtag ttgctagtaa
    77881 ggtctaacac aaaacatcaa aggctttcaa aaacaactcc taagcttaaa aaaggctgtt
    77941 taacagattt gttgagctta agggagattg ccatgatttt ctaatcattt gatgtttaaa
    78001 atgataaaaa catgaacttg aaatgaaaga ctgctagtag taaactaagc ttatcaaaaa
    78061 aatgaaaatg gagtggtttt atgaaaacaa aatcaaaacg ctttttaaac ctagcaaccc
    78121 tttgcttggc cctactagga acaactttgc taacgacaca gcccgtaaag gcggaggaag
    78181 ggcaaaataa gcaacaggaa gtgtctgtca gcagccagtc tggacaatct gaaggagaag
    78241 acttatcgga ggattcagac gataagtatc catatgctga aggtcatgaa gctggccaag
    78301 attttggata taaagaagga ttaaaggcag gttggcagga agctgatcct aaagaaccaa
    78361 aggatattcc tgaacccgaa tttaatccat atgaatctga aggagaagac cgtaatgatt
    78421 tcaaagaagg atttcgcgac ggatatcctg caggatacgt tgaaggatgg agaaaaagtc
    78481 atccattatg ggcaatcgtt caagatgtct tgccttggct ttataatttt ttaagataaa
    78541 atagtgtata atactagccc tgacagttct ggcctacgcc tactatgacc tacctaaacg
    78601 acatccaaag agtgagggaa ctacgaggct gggcaaaaac tagcttcgac ataaaaccac
    78661 actgcagttc tttccatctt gaaaatactc aagatgaaag aattacgtgt ggtttttgat
    78721 ttttaaaagt tttagtaact aaattctttg ccaattttgt gagattcatg ctcatgaaga
    78781 gacatccgac ctccgtttgg acgaattaat tgcctcaaac gtgaactctg cgtacgctaa
    78841 aaatactctt caatctacca aaaacgggtt cgacatcgat tttccgttta gcgtagatat
    78901 gagagccctc ttcgctatgc aattgttcct ttatgagttc cttaaaataa ttccaagtcg
    78961 gattataatg gatttgtttc tgacgatcac tttctgtttt agccagttat tctagctctg
    79021 gcgtgtcctg tattttatct gcctcgtaaa ttttgaaatc tcgtttaaac ccgtacttgt
    79081 cgactcgacg agagtaagat ttaaaagaat agacaatccc atcaggtttt ataatctggt
    79141 ctgtctctcc aagatagtcc cagttagctg ggttctccgc actgttctta tatttctgag
    79201 tcaattcctt ttggtacatg ccatagggaa taagagctgt tttctccaac tcatcaataa
    79261 taaaacgata attttcttcg ctaccataac ctgcatcggc cataatattt tggaataagt
    79321 ccaatgtttg attggattgg aggaagggtc ttagggttct gatatcggtt gggtttgcaa
    79381 agatatcata agcaaggata tattgaccat tagtcgctgc ttgaacatta taacctggtt
    79441 tcagctgacc attcttcata tgactcgctt ttatatttta aggcttgtga agaaatagtc
    79501 tctcccagac tatttctttc ctttattcgc ataaaggtag cgtcaggatc agttctagaa
    79561 aaagagttgc gttcttgaag aatttctctc gcctcctcat atcgctgttt acgaggaata
    79621 taatctttcc tcaactgatt gcggaatttc ttgatgcgct gccgtttttg tgtattaacc
    79681 gaactacctt ttatcacttt tggttcttga gtgatcgctt tttctacttc atcaagggct
    79741 tctttccctt tttcaagcag cacgttaagt ccttcgctag tttcatattc ttcttttgaa
    79801 agggctaaag ttaccccttc ttgaatgagc ttatcataga gttaagaaat gtttccattg
    79861 tctctcaaga gcaaggtaaa ataaataaaa gtagtcttaa tcaggttatt ggcaggctta
    79921 ctcgaacggt cttattgatc gttttatagc taacataagt gtcttgactt agccatttca
    79981 tcggaataac ttcttcgttc atctgcacca ttttacgacc agaaaagacc tgacgactat
    80041 aggcaaagag tgtcattttt aaaagcatag caagatgaaa agcaggtcgg ccagtatgag
    80101 acttttcttc aagtaaaatc aacgctgaag actttttgga ttcccataat tgttaactac
    80161 atcattttag agcaatcata tagagactaa gacataattt taccaaagtt ttgaaggcta
    80221 ttgaagagta actttcttct ttaatatatt taataaattt tcgcaagttg tcaagtcaag
    80281 tgcaacgagt tcatagataa ctagagttcc agaggttaag ctgttcgggc tagctcaaac
    80341 aggaatgttg aggattgata ttgaagaagt cgtcttgggc gttcattgat ggctgtaaga
    80401 taattgtcaa attcttcaga ggttagtgaa ttaagagaga ttcctttagg gacatattct
    80461 ctgaggaggc cattgaagtt ctcgtttgtt cctctctcat gtgaagaata gggatgtgca
    80521 aagtaaacgt caacagcttc taaggttgag agtaaactaa actcagaacc attatccgct
    80581 gtggtggatg caataggata ctgactgatg agatgcgtga cagcacggtt aatggtttgg
    80641 gactgcttgt cttctagctt aactcctagt gcatagcgtg tctggcgctc taccaaagtc
    80701 aacataacag cttcaccttt ggttttcttg ccgagaacca aatcaatctc ccaatgccca
    80761 aattcagaac gatcattaat atattctgga cgttcttcga tagatttacc taaagtcttc
    80821 ttattcgttt tcatgacttt cttagaacgt tttctgatac taaccatctt aggcaaatcg
    80881 attggtttca tagacacgtt gtctactatc agcgaaatag gcgttgaaga aggtttgttt
    80941 tccattctta tcctttacct gatctactga accacgtttt atttctcggc agattgtaga
    81001 acggtgacta cccagaagtc gagcaatctc aacgggtttc tttcccattt taagataagc
    81061 agcaatttca ccgcgttctg tggctgaaag atgagaatag gaggattttc tggtaaaatt
    81121 aatattagac atgttcatct gct
//
LOCUS       DATURV010000003        68813 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DATURV010000003 DATURV010000000
VERSION     DATURV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68813
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.003"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            7..933
                     /locus_tag="VCF75_000154"
     CDS             7..933
                     /locus_tag="VCF75_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP2545753.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLINRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIRENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            987..1072
                     /locus_tag="VCF75_000155"
     tRNA            987..1072
                     /locus_tag="VCF75_000155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1021..1023,aa:Leu,seq:aag)
     gene            1280..2161
                     /locus_tag="VCF75_000156"
     CDS             1280..2161
                     /locus_tag="VCF75_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEP2545754.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            2543..2731
                     /locus_tag="VCF75_000157"
     CDS             2543..2731
                     /locus_tag="VCF75_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEP2545755.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            2883..3248
                     /locus_tag="VCF75_000158"
     CDS             2883..3248
                     /locus_tag="VCF75_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP2545756.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            3248..4912
                     /locus_tag="VCF75_000159"
     CDS             3248..4912
                     /locus_tag="VCF75_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEP2545757.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKTKA
                     EAKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            5149..6039
                     /locus_tag="VCF75_000160"
     CDS             5149..6039
                     /locus_tag="VCF75_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEP2545758.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            6269..7123
                     /locus_tag="VCF75_000161"
     CDS             6269..7123
                     /locus_tag="VCF75_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEP2545759.1"
                     /translation="MKSKKVVSVISLTLSLFLVTGCAKVDNNKSVNRKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
     gene            7316..8512
                     /locus_tag="VCF75_000162"
     CDS             7316..8512
                     /locus_tag="VCF75_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEP2545760.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAEFDQALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8775..9326
                     /locus_tag="VCF75_000163"
     CDS             8775..9326
                     /locus_tag="VCF75_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP2545761.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            9393..9764
                     /locus_tag="VCF75_000164"
     CDS             9393..9764
                     /locus_tag="VCF75_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP2545762.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            9802..11148
                     /gene="glnA"
                     /locus_tag="VCF75_000165"
     CDS             9802..11148
                     /gene="glnA"
                     /locus_tag="VCF75_000165"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEP2545763.1"
                     /translation="MAITVADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPIEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFISQWEIDHYIHNY"
     gene            complement(11376..13058)
                     /locus_tag="VCF75_000166"
     CDS             complement(11376..13058)
                     /locus_tag="VCF75_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP2545764.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(13060..13290)
                     /locus_tag="VCF75_000167"
     CDS             complement(13060..13290)
                     /locus_tag="VCF75_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEP2545765.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            13573..14271
                     /gene="tsaB"
                     /locus_tag="VCF75_000168"
     CDS             13573..14271
                     /gene="tsaB"
                     /locus_tag="VCF75_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEP2545766.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHAPLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            14243..14698
                     /gene="rimI"
                     /locus_tag="VCF75_000169"
     CDS             14243..14698
                     /gene="rimI"
                     /locus_tag="VCF75_000169"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEP2545767.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIW
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLGSIESD
                     IFLEVRESNHRAQGLYQKFGFKFIGKRPDYYRNPIETALLMKREGKNDR"
     gene            14688..15716
                     /gene="tsaD"
                     /locus_tag="VCF75_000170"
     CDS             14688..15716
                     /gene="tsaD"
                     /locus_tag="VCF75_000170"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEP2545768.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGDELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(15887..16156)
                     /gene="rpsN"
                     /locus_tag="VCF75_000171"
     CDS             complement(15887..16156)
                     /gene="rpsN"
                     /locus_tag="VCF75_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEP2545769.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(16377..17135)
                     /locus_tag="VCF75_000172"
     CDS             complement(16377..17135)
                     /locus_tag="VCF75_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2545770.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
     gene            17376..18155
                     /gene="udp"
                     /locus_tag="VCF75_000173"
     CDS             17376..18155
                     /gene="udp"
                     /locus_tag="VCF75_000173"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEP2545771.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            18176..19378
                     /locus_tag="VCF75_000174"
     CDS             18176..19378
                     /locus_tag="VCF75_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEP2545772.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            19408..20079
                     /gene="deoC"
                     /locus_tag="VCF75_000175"
     CDS             19408..20079
                     /gene="deoC"
                     /locus_tag="VCF75_000175"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEP2545773.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECEDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAKCQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAETFIALGASRLGTSRIIKIVKNEAT
                     KPDSY"
     gene            complement(20174..21343)
                     /locus_tag="VCF75_000176"
     CDS             complement(20174..21343)
                     /locus_tag="VCF75_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP2545774.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVRLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGKVLTSTEVVSVKKQDDLFYLKSADQTFSCHKLVV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            21548..22078
                     /locus_tag="VCF75_000177"
     CDS             21548..22078
                     /locus_tag="VCF75_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEP2545775.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            22127..22714
                     /locus_tag="VCF75_000178"
     CDS             22127..22714
                     /locus_tag="VCF75_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEP2545776.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHIIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            22866..23603
                     /locus_tag="VCF75_000179"
     CDS             22866..23603
                     /locus_tag="VCF75_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP2545777.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTAGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKLQWKMGISIFLAIVSYVGLILL
                     SNYLQKFWLVYVAMGLFLPGFSWLVIYFRKQFIYLCPHCHQKFEPSFNEFALAGHTPR
                     TRKLTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(23660..23959)
                     /locus_tag="VCF75_000180"
     CDS             complement(23660..23959)
                     /locus_tag="VCF75_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEP2545778.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(24152..24526)
                     /locus_tag="VCF75_000181"
     CDS             complement(24152..24526)
                     /locus_tag="VCF75_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2545779.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELAMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            24696..24917
                     /locus_tag="VCF75_000182"
     CDS             24696..24917
                     /locus_tag="VCF75_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2545780.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(24997..27279)
                     /locus_tag="VCF75_000183"
     CDS             complement(24997..27279)
                     /locus_tag="VCF75_000183"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEP2545781.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKANIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDRYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIVNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFETSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRAFKAALFKGKANQALVDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGNTLRVILYTTDFEHTVRDNS
                     NYTLTIDLSQSQLIVPIASN"
     gene            complement(27301..27960)
                     /locus_tag="VCF75_000184"
     CDS             complement(27301..27960)
                     /locus_tag="VCF75_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP2545782.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            28126..29322
                     /locus_tag="VCF75_000185"
     CDS             28126..29322
                     /locus_tag="VCF75_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2545783.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMIIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTFLYLLKTK
                     TRQVVS"
     gene            29794..30642
                     /locus_tag="VCF75_000186"
     CDS             29794..30642
                     /locus_tag="VCF75_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP2545784.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(30978..31874)
                     /locus_tag="VCF75_000187"
     CDS             complement(30978..31874)
                     /locus_tag="VCF75_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEP2545785.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYFGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            32172..32924
                     /locus_tag="VCF75_000188"
     CDS             32172..32924
                     /locus_tag="VCF75_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEP2545786.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            32909..33859
                     /locus_tag="VCF75_000189"
     CDS             32909..33859
                     /locus_tag="VCF75_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEP2545787.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKVYYPSIADTTVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKVLNHLTQKENKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(34039..36366)
                     /gene="pflB"
                     /locus_tag="VCF75_000190"
     CDS             complement(34039..36366)
                     /gene="pflB"
                     /locus_tag="VCF75_000190"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEP2545788.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            36575..37669
                     /gene="dinB"
                     /locus_tag="VCF75_000191"
     CDS             36575..37669
                     /gene="dinB"
                     /locus_tag="VCF75_000191"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEP2545789.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDLVEPMSIDEAYLDVTDNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKVRGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            complement(37762..38244)
                     /locus_tag="VCF75_000192"
     CDS             complement(37762..38244)
                     /locus_tag="VCF75_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545790.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTLSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(38335..40788)
                     /locus_tag="VCF75_000193"
     CDS             complement(38335..40788)
                     /locus_tag="VCF75_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEP2545791.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKIADALAENLGIESDSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTYFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQAVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTATLEDDSQKSSSQSTNSNPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDIALFFQKKS"
     gene            complement(40846..41439)
                     /gene="lepB"
                     /locus_tag="VCF75_000194"
     CDS             complement(40846..41439)
                     /gene="lepB"
                     /locus_tag="VCF75_000194"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP2545792.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(41450..42352)
                     /locus_tag="VCF75_000195"
     CDS             complement(41450..42352)
                     /locus_tag="VCF75_000195"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEP2545793.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNTYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHPFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNAISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            42509..42817
                     /locus_tag="VCF75_000196"
     CDS             42509..42817
                     /locus_tag="VCF75_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545794.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            42820..43365
                     /locus_tag="VCF75_000197"
     CDS             42820..43365
                     /locus_tag="VCF75_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEP2545795.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            43514..45853
                     /locus_tag="VCF75_000198"
     CDS             43514..45853
                     /locus_tag="VCF75_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEP2545796.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNISFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISNDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPILADKGSKIAVFNNIFADIGDEQSIEQNLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRHKHVKHFAYAPQNAGGSGATIVTLG"
     gene            45857..46357
                     /locus_tag="VCF75_000199"
     CDS             45857..46357
                     /locus_tag="VCF75_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP2545797.1"
                     /translation="MESYEHFYAKLSQPFRKTPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            46438..46752
                     /gene="trxA"
                     /locus_tag="VCF75_000200"
     CDS             46438..46752
                     /gene="trxA"
                     /locus_tag="VCF75_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEP2545798.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQTPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(46804..47391)
                     /locus_tag="VCF75_000201"
     CDS             complement(46804..47391)
                     /locus_tag="VCF75_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2545799.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSHQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFKRDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQPYKEILAVSSGKLLDEIEKREE
                     RAKLPYQNPLIVTVFFLITVAIFFASRFMFTWLFH"
     gene            complement(47568..48692)
                     /gene="mutY"
                     /locus_tag="VCF75_000202"
     CDS             complement(47568..48692)
                     /gene="mutY"
                     /locus_tag="VCF75_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEP2545800.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKHFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQSGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            48890..49183
                     /locus_tag="VCF75_000203"
     CDS             48890..49183
                     /locus_tag="VCF75_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545801.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            49356..49646
                     /locus_tag="VCF75_000204"
     CDS             49356..49646
                     /locus_tag="VCF75_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEP2545802.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGANVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            49668..50159
                     /locus_tag="VCF75_000205"
     CDS             49668..50159
                     /locus_tag="VCF75_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP2545803.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            50324..50563
                     /locus_tag="VCF75_000206"
     CDS             50324..50563
                     /locus_tag="VCF75_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEP2545804.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(50696..51352)
                     /locus_tag="VCF75_000207"
     CDS             complement(50696..51352)
                     /locus_tag="VCF75_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEP2545805.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQRAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(51484..52428)
                     /locus_tag="VCF75_000208"
     CDS             complement(51484..52428)
                     /locus_tag="VCF75_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEP2545806.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            52631..55459
                     /gene="uvrA"
                     /locus_tag="VCF75_000209"
     CDS             52631..55459
                     /gene="uvrA"
                     /locus_tag="VCF75_000209"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEP2545807.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLKEGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            55574..56647
                     /locus_tag="VCF75_000210"
     CDS             55574..56647
                     /locus_tag="VCF75_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP2545808.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLNVVAELLGADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRLIKDVSEIETIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIAASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKASAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFLGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLAPKELIVL"
     gene            56682..57143
                     /locus_tag="VCF75_000211"
     CDS             56682..57143
                     /locus_tag="VCF75_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEP2545809.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            57239..57796
                     /gene="efp"
                     /locus_tag="VCF75_000212"
     CDS             57239..57796
                     /gene="efp"
                     /locus_tag="VCF75_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEP2545810.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            57842..58231
                     /locus_tag="VCF75_000213"
     CDS             57842..58231
                     /locus_tag="VCF75_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP2545811.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            58224..58676
                     /gene="nusB"
                     /locus_tag="VCF75_000214"
     CDS             58224..58676
                     /gene="nusB"
                     /locus_tag="VCF75_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEP2545812.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(58817..59782)
                     /locus_tag="VCF75_000215"
     CDS             complement(58817..59782)
                     /locus_tag="VCF75_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2545813.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEILKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(59787..61226)
                     /locus_tag="VCF75_000216"
     CDS             complement(59787..61226)
                     /locus_tag="VCF75_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEP2545814.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGRYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDLDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGRA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTMRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            61468..63351
                     /locus_tag="VCF75_000217"
     CDS             61468..63351
                     /locus_tag="VCF75_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP2545815.1"
                     /translation="MDNRQIAAEVIEALGGRENVRSVAHCATRLRVMVYDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSILGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGVAFAIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSTDYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            63582..66554
                     /locus_tag="VCF75_000218"
     CDS             63582..66554
                     /locus_tag="VCF75_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEP2545816.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKEKVPEKVKEILAKAQQADKQAQELTKMKIPEK
                     IPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLFW
                     KELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIVD
                     EYVYKYNLDGLDVDVEHDSIPKVNGKASDENLKRSIDVFEEIGKLIGPKGVDKSRLFI
                     MDSTYMADKNPLIERGAPYIDLLLVQVYGARGEQGEFQNDTKLVTETPEERWQGYSKY
                     IRPEQYMIGFSFYEERAGSGNLWYDINSRKDDDKANGINTDITGTRAERYARWQPKTG
                     GVKGGIFSYAIDRDGVAHQPKQIAEKDKQNVKNNQPQIPEITDNIFHSDYSVSKALKT
                     VMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEGLNK
                     FKKLAQLDLIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSLK
                     VSGLTGLKELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDTMLS
                     TISNHVGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVAEGNIDLQSQLLFGTVTNQGT
                     LINSEADYKAYQNHKIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLATD
                     KEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADSQAALKAFDG
                     VIEYNPISTQNRASIIFEMKDPSLAKYWRLFNDSSKGEDDYIKEAKLEVFTGQLNAEA
                     DVKTSLEKSDDWQTVSTYSGQEQVFSHALDNISAKYWRVTVDTKGGNYSWPSLPELQI
                     LGYPLPNADTIMKTVTTAKELSQQKDKFSQKILDELKIKEMALETSLNSKIFDVTAID
                     ANAGVLKDCIEKRQLLKK"
     gene            66818..67702
                     /locus_tag="VCF75_000219"
     CDS             66818..67702
                     /locus_tag="VCF75_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP2545817.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIAQT
                     IAYFKTFEADLAGMAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     LHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMAQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            67797..68750
                     /gene="manA"
                     /locus_tag="VCF75_000220"
     CDS             67797..68750
                     /gene="manA"
                     /locus_tag="VCF75_000220"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEP2545818.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
BASE COUNT        21137 a        12011 c        14463 g        21202 t
ORIGIN      
        1 cttggtatga aaactattcg aatagcaaag tatttaggaa ttctattcct tttgataact
       61 cttattagcg taggtgctag tttttatttc tttcatgttg cacaaataag agaagagaaa
      121 tcgtttatta ataataagaa acggagtaca aataatccat tatacccagc tgaacagtct
      181 tttgacgctt taccttacga aaaacgtcaa ctaataaatc gtgggttaaa acaagtgggg
      241 tggtacttac cagctgctca aaaaacaaaa aagacagcta ttgttgttca tggttttacg
      301 aatgacaaag aagatatgaa gccatatgcc atgctttttc atgatttggg ctataatgtc
      361 ttaatgccag acaatgaggc ccatggggaa agtgaaggga acttgattgg ttatggctgg
      421 aatgaccgcc ttaatgtcat ggcttggaca gaccaactga ttagggaaaa ccctgaaagc
      481 caaatcacac tctttggctt atctatgggt gctgcaacag taatgatggc aagtggtgag
      541 cgattgcctg cgcaagtcac ctccctcatc gaagattgcg gttataccag tgtttgggac
      601 gaattgaagt ttcaggccaa ggctatgtac aacttgcctg cctttccttt actctatgaa
      661 gtctctgcct tatctaagat tcgagcaggt tttagttacg gagaagcgag ctcagtgaaa
      721 cagctggcta aaaataaacg tccaacttta tttatccatg gtgataagga tgattttgtt
      781 cctacaaaaa tggtttatga caattataag gccacgaaag gtcctaagga aatcttgatt
      841 gttaaagggg caaaacacgc caaatccttt gaaacaaacc cagaacaata ccagaaaaaa
      901 attgccgctt ttttgaaaaa agttgagaaa taagtattga cagttaaact cacagttgct
      961 ataatgattt atgttactga tgagtagcgg ggatggcgga attggcagac gcgcaggact
     1021 aaggatcctg tgaccgcttt aggtcgtgtg ggttcaagtc ccactctccg cataatgtaa
     1081 gaggtgaatc attgattcgt ctctttttgt ttggttaaat gaaggataag aaggcataga
     1141 tttaccggag aaaacttttg ataatctgct tttggaaaac gctttaagct tgtgaaagac
     1201 tagaaattta tggaaaattg tgctagaatg aacaatgtaa acggggagtt atcccgaaaa
     1261 ataagaggag gccttacaaa tggcaatcgt ttcagcagaa aaatttgtcc aagcagctcg
     1321 tgaaaacgga tatgctgttg gtggatttaa cacaaacaac ttagagtgga ctcaagcaat
     1381 cttgcgtgca gcagaagcta aacaagctcc agttcttatc caaacatcaa tgggtgcagc
     1441 aaaatacatg ggtggttata aagtatgtca atctcttatt actaaccttg ttgagtcaat
     1501 gggtatcact gttcctgttg ctatccacct tgatcatggt cactacgaag atgctcttga
     1561 gtgtatcgaa gtaggttaca cttcaatcat gtttgacggt tcacaccttc cagttgaaga
     1621 aaaccttgct aaaactgcag aagttgttaa aattgcacat gctaaaggcg tttcagttga
     1681 agctgaagtt ggtacaatcg gtggtgaaga agacggtatc atcggtaaag gtgagctcgc
     1741 tccaatcgaa gatgctaaag caatggttga aactggaatt gacttcttgg cagctggtat
     1801 cggtaacatc cacggtccat acccagaaaa ctgggaaggt cttgctcttg accacttaga
     1861 aaaattaaca gcagctgtac caggtttccc aatcgtattg cacggtggtt ctggtattcc
     1921 tgacgatcaa atcaaagaag caatccgtct tggtgttgct aaagttaacg ttaacactga
     1981 aagtcaaatc gctttctcta acgcaactcg tgaatttgct cgtaactacg aagctaacga
     2041 agcagaatat gatggtaaaa aattatttga cccacgtaaa ttcttggctc caggtatgaa
     2101 agctgttcaa ggtgctgttg aagaacgtat cgatgttttc ggatcagcta acaaagcata
     2161 atttagagta ttaaataccc taaagccctc ttgagggctt ttttagatac aattgacatc
     2221 ttaaaatgga taccagtttc tgtgaaacaa tctttgattg tgaacaaaca aagcataatt
     2281 tagagtatta aaatttggac cctgctatct atggtaaatc tttttgttat tgttaaagag
     2341 gttaagcgga atgttaatat gttttagcta aaaaatgtag tgaaaaatga gtacgtagac
     2401 tattgtaata gtaattccgt aaaattgttg aaaaagaaaa tgggaaatac cttgtcaaat
     2461 taagcaccct atgatagaat agataggtat gtggtgctag cacatccgta aacatattct
     2521 acgtaggagg aaacagatca aaatggctaa agtatgttat tttacaggac gtaaaaccgt
     2581 ttctggaaac aaccgttcac acgcgatgaa ccaaacgaaa cgtacagtta aaccaaacct
     2641 tcaaaaagtt actatccttg ttgatggtaa acctaaaaaa gtttgggctt ctgctcgtgc
     2701 gcttaaatct ggtaaagtag aacgcatcta atagaaataa gccttagggc tttttttatt
     2761 agcttatttc tatccataag ctagtcaggg taataaacgc tattgttaac tattctatct
     2821 cttttacatt acttctaaaa tatgataaaa taggatgata aaaacttttt gaggtagtaa
     2881 ctatgactgt aaaaattaat acaaaagatg gtctaatcga gctatccgac gatgtgattg
     2941 caactgtcgt gggaggatca gcaacggaaa tttttggtgt tgttggaatg gccagcaaaa
     3001 gtgcaataaa agacaatttt caatcactac ttcgcaaaga aaactacgct aagggtgttg
     3061 tagtaaaatc aacagatctg ggtatttctg ttgatgtcta cactgtgatg agttatggtg
     3121 tgaaaatcag tgaagtctct aaaaatatcc aagaacgcgt gaaatttaac cttgaaagtc
     3181 agcttggtct cactgctgat atggtcaatg tttacgtgca aaatattaag gttgtaggag
     3241 aaaattagtg tcaaatatta caacaagctt attccaagaa atggtccaag cggcagcgac
     3301 tcgccttggc aaacaagcag aatatgttaa ttccttaaat gtttttcctg ttccagatgg
     3361 tgatacagga acaaacatga gcatgactat ggacaatggt gccaaggaag ttgctgataa
     3421 acctgcgtca actgtgggag aagttggaca aatgttatca aaaggtcttt tgatgggagc
     3481 gcgtggaaat tctggagtta ttacttcaca actttttcgt ggttttggtc aaagtattaa
     3541 gggaaaagat gagctgacag gtaaagattt ggcacaagcc tttcaagtgg gtgtagaagt
     3601 tgcctacaaa gcagtgatga aacctgttga agggacaatc ttgacagtat ctcgtggagc
     3661 agcaaccgct gccttaaaaa aagcagatct tacagacgac gccgtggaag tcatgcaagc
     3721 tgctttagac ggtgccaaag gtgcccttgc taagacacct gatttattac cagtccttaa
     3781 agaagttggt gtggttgatt caggcggtca aggtcttgtc tttatctatg aaggtttcct
     3841 atctgctttg aatggcgact atgtgacttc agcagacttt aaagcgactc ctgctaacat
     3901 gtctgaaatg attaatgctg agcatcacaa atccgttgtt ggtcatgtgg ctactgaaga
     3961 cattacctac ggttattgta ctgaaatcat ggttgccctt aaacaaggcc caacctatgt
     4021 gaaagaattc aactatgatg agtttcaagg ctaccttagt ggccttggtg actcgctgtt
     4081 agtggtcaat gatgatgaga ttgttaaagt tcacgtccat actgaagacc ccggacttgt
     4141 gatgcaagaa ggtcttaaat atggttcgct catcaagatt aaagtggaca atatgcgtaa
     4201 ccagcatgaa gcacaggttc aaaagactga tgtcgaaaaa accaaggctg aagcaaaaga
     4261 ctttggcttg attgcagtag tagctggaga gggtttatct gaaattttca aggcgcaagg
     4321 tgttgattac gtgatttctg gtggtcaaac catgaatcca tctacagaag atattgttaa
     4381 agctattgaa gcagttaatg ccaaacaagt cattatcttg ccaaacaata aaaatatctt
     4441 tatggctgcc caatctgctg cagaagtggt ggatatcccg gctgctgttg ttgcaacacg
     4501 tactgttccg caaggattta ccagcttgtt ggcctttgat ccttcaaaat ctttagagga
     4561 caatgtagca gatatgtcaa ctagtctttc tgacgtggtc agtggaagcg ttactttggc
     4621 agtccgtgac accaccattg atggtttaga aatccatgaa aatgatttct tgggaatggt
     4681 tgatggtaaa atcatcgttt caaacccaga catggaagca actctaaaag ctgcctttga
     4741 aaagatgatt gacgaagata gcgagattgt gactatcttt gttggtgaag aaggtgatca
     4801 agacttagca gaagaacttg ctgggtattt aggagagact tacgaagatg tggaagttga
     4861 gatccatcaa ggagatcagc ctgtttaccc atatctcatg agtgtagaat aatagaatac
     4921 aataaaaaaa gaggagttag gcaaaggtct gactcttttt gtctatattg atgtcgtaat
     4981 gagtggtttt agtgatggta agcccctaaa aaatgagggt aatccatcac aaagctaatg
     5041 gttacgattt aatttgacaa aacctagaaa aacattataa ttgtattata acaataagtc
     5101 aagaactgaa aacggttcag ttctaagata tgacaaggag gagattttat gttaggacca
     5161 tttatcttta ttgcttttgg ggttattgta atcctagcta ttgttgccag taccctttat
     5221 gttgttcgtc aacaatctgt tgccatcgtg gaacgttttg gtaggtacca aaaaacagcc
     5281 acgagtggta ttcatgtccg ccttccgttt gggattgaca aaattgctgc gcgtgtgcag
     5341 ctgcgtcttt tacagtcaga aatcatcgtt gaaaccaaaa ccaaggacaa tgtgtttgta
     5401 actttgaatg ttgccactca gtatcgtgtt aacgaacaaa atgtaacaga tgcttactac
     5461 aaattaatga aacctgaatc tcaaatcaag tcttatattg aagatgcctt gcggtcatcc
     5521 gtgccgaagt tgaccttaga cgaattgttt gagaaaaaag atgaaattgc cttggaagta
     5581 caacatcaag tggcagaaga aatgtcaacc tatggatata ttattgtcaa aaccttgatt
     5641 accaaggtgg agcctgatgc tgaagtcaaa cagtcaatga atgaaatcaa tgcggctcaa
     5701 cgcaaacgtg tagctgctca agagttggca aacgcagata aaattaaaat tgtgacagca
     5761 gcagaagctg aggctgaaaa agatcgattg cacggggtag ggattgccca acagcgtaag
     5821 gccattgtgg atggtttggc ggagtctatt caagagctta aagaagcgaa tatttcctta
     5881 aacgaagaac aaatcatgtc cattttgttg accaaccaat acctggatac cctcaatacc
     5941 tttgcggcaa aaggaaacca aacccttttt ctaccaaata caccaagtgg agtagaggat
     6001 atccgtacac aagttttatc tgcccttaaa acaaaataac gtgtaaaagc ttggctaaaa
     6061 agttgctcaa tactggattt agcagaaggc tatcataact tgtgaagtgg caaacgacga
     6121 aatgaataca ttacttttat tggagaaatg aaagtaaaaa aagccttttt catgattaac
     6181 aacatagtat tgattaaatg ttaagttgta ttgactttta aattaaataa gtttaaaatc
     6241 aatttaataa gcaaaaagga gattttcaat gaaatccaaa aaagttgtta gtgttatatc
     6301 acttacctta tccctttttt tggtgacagg ttgtgctaaa gttgataaca acaaatcagt
     6361 taatcgtaag cctgctacta aacaaaccta taatagctat agtgatgacc aattaagatc
     6421 gcgtgaaaat accatgtctg ttttatggta ccagcgagct gcggaaactc aagcgcttta
     6481 tttacagggg tatcagttag caacggatcg cttaaaagaa caactcaata aaccaacgga
     6541 taaaccttat tcaattgtat tagatattga cgaaacagtt cttgataata gcccttatca
     6601 agctaaaaat gttttggaag gaacaggatt tacacctgaa agctgggatt attgggtaca
     6661 aaagaaagaa gcaaaaccgg ttgctggtgc taaagacttt ttgcaatttg cagaccaaaa
     6721 tggtgttcaa atttactaca tttcagacag atcaactact caagtagatg ctacaatgga
     6781 aaatctccaa aaagaaggta ttccagtaca aggtcgtgat catcttctat tcttagaaaa
     6841 aggcgtaaaa tcaaaggaga gtcgtcgtca aaaggtcaaa gaaacaacta atgtaacgat
     6901 gctatttggt gataatcttc tagattttgc tgatttttca aaaaaatctc aagaagatag
     6961 aacagcttta ttatcagatt tgcaagaaga gtttggaaga cgctttatca ttttccctaa
     7021 tcctatgtat ggttcatggg agggtgccat ttataaaggt gaaaagctgg atgtgcttaa
     7081 gcaactagag gaacgccgta aaagtttaaa aagctttaaa taaagtaatt gaaaaagagg
     7141 tgcaaaagaa aatgtaacct ctttttaagt actattattt tctgaaaata gggttaatga
     7201 ctgaaaaaaa tgttgatttt gtgaaatgat tattttatta ttttcatatt ttatctttat
     7261 gctaggaatt gtggtataat ggaggagtat aaaaaatatt aaggagtcta ttcacatggc
     7321 taaattgact gttaaagacg ttgatttgaa aggtaaaaaa gtcctcgttc gtgttgactt
     7381 taatgtgcct ttgaaagacg gcgttatcac taacgacaac cgtatcactg cggctcttcc
     7441 aacaatcaag tatatcatcg aacaaggtgg tcgtgctatc ctcttctctc accttggacg
     7501 tgttaaagaa gaagctgaca aagaaggaaa atcacttgca ccggtagctg ctgatttagc
     7561 tgctaaactt ggtcaagatg ttgtattccc aggtgttact cgtggttcaa aattagaaga
     7621 agcaatcaat gctttggaag atggacaagt tcttttggtt gaaaacactc gttttgaaga
     7681 tgttgacggt aagaaagaat ctaagaatga cgaagaactt ggtaaatact gggcttcact
     7741 tggagatgga atcttcgtta acgatgcatt tggtacagca caccgtgctc acgcatcaaa
     7801 cgtaggtatt tcagcaaacg ttgaaaaagc tgtagctggt ttccttcttg aaaacgaaat
     7861 tgcttacatc caagaagcag ttgaaactcc agaacgtcca ttcgtagcta ttcttggtgg
     7921 ctcaaaagtt tctgataaga ttggtgttat cgaaaatctt cttgaaaaag ctgataaagt
     7981 tcttatcggt ggtggtatga cttacacatt ctacaaagct caaggtatcg aaatcggtaa
     8041 ctcacttgta gaagaagaca aattggatgt tgctaaagac ctccttgaaa aatcaaatgg
     8101 taaattgatc ttgccagttg actcgaaaga agcaaacgca tttgctggtt acactgaagt
     8161 tcgcgacact gaaggtgaag cagtttcaga aggcttcctt ggtcttgaca tcggtcctaa
     8221 atcaatcgct gagtttgacc aagcacttac tggtgctaaa acagttgttt ggaacggacc
     8281 tatgggtgtc tttgaaaacc ctgacttcca agctggtaca atcggtgtca tggacgctat
     8341 cgttaaacaa ccaggcgtta aatcaatcat cggtggtggt gattcagcag cagctgctat
     8401 caaccttggc cgtgctgaca aattctcatg gatcagtacc ggcggaggcg ctagcatgga
     8461 acttctcgag ggtaaagtat taccaggttt ggcagcattg actgaaaaat aatagtcatt
     8521 ataatatata gtaaagggca tgctgatgca tgtctttttt tagttgatca tgtacagtta
     8581 ttgaactttc cctaacttca cttatcattt actagttttt agatatagtg gtatttggct
     8641 gacactgcaa ttttcaaaaa tttatgacga tttcatatca attggtttat aaaagatatc
     8701 ctaatcataa ataaaacgtt taccaaaagg gttttatgga ttgatatgtt aaaatagata
     8761 gtgaagggat aggtatgaaa acgttaagaa aattattatc taattacaag tttgatatta
     8821 aaaagtttaa attgggaatg agaacattga aaacaggctt atcggtcttt ttagttttat
     8881 tggtttttca tttatttggt tggaaagggc tccaaattgg tgctctaact gctgtattta
     8941 gtttaagaga agactttgat aaaagcgtgc actttggctt ttctagaatt attggtaata
     9001 gtataggtgg cctactctcc ttagtatttt ttgcattcaa tgagatattt catcaagctt
     9061 tttgggttac tttattaatt gttcctattt gtaccatgtt atgtatcatg gttaatgttg
     9121 catgtaataa taaatcaggt attattgggg cagtggctgc tcttttgatt attacactgt
     9181 ctattccaac aggacaaacg tttatttatg tgacttcgcg ggtatttgaa acattttgtg
     9241 gcgtctttgt agctatttta gttaatactg atgttgaact tattaaaaat aaatggttta
     9301 acaaaaaaca cagtgatgtt agataatata acatatatac ttgacaggtt atttttttca
     9361 gcctataata aggaagatga aaggagtttg tcatgaaaga aaaagaactt agacgctcaa
     9421 tggctgtttt tcctattgga acggtgatga cattaacaga tctctctgcg agacaaattc
     9481 gctattatga agatcaaggt ttaattaagc ctgaacgaac gcaagggaat cggcgtatgt
     9541 tttccttaaa tgacatggac cgacttttgg aaattaaaga ttttttgtct gagggattaa
     9601 atattgcagc tattaaacgc gaatatgtcg agcgtcaagg taagttaatg caaaaacaaa
     9661 aagctctaac agatgcagat gtcagacgga tcttacatga tgaaatgctg acacaaagcg
     9721 gtttttctac tccctcacag catattggta attttcgtat ttaagatatg aattgtataa
     9781 aatttaaagg agacctattt aatggcaatc acagtagctg acattcgtca tgaagtcaaa
     9841 gaaaaaaatg taacgtttct tcgcttgatg ttcactgata tcatgggcgt tatgaaaaat
     9901 gtggagattc ctgcaacgaa agaacagtta gacaaagtat tgtctaacaa ggttatgttt
     9961 gatggttcat ctatcgaagg ttttgtacgg atcaatgagt cagatatgta cctttacccc
    10021 gatttagaca cttggattgt ttttccctgg ggagatgaaa atggagcagt tgcaggttta
    10081 atttgtgata tttatacagc agaaggaaag ccttttgcag gagatcctag aggaaattta
    10141 aaaagagccc tgaaacacat gaacgagatc ggctacaaat catttaatct tgggccagaa
    10201 ccagaatttt tcctttttaa gatggatgat aaaggtaatc cgacacttga agttaacgat
    10261 aatggtggtt attttgattt agcgccaatt gacttagcag acaacacgcg ccgtgaaatt
    10321 gtgaatattt taacgaaaat gggttttgaa gtggaagcta gtcatcatga agtggctgtt
    10381 ggtcaacatg agattgattt taaatatgca gatgttttga aagcttgtga taatattcaa
    10441 atttttaagc tagttgtaaa aacgattgcc cgtgaacatg gactttatgc tacttttatg
    10501 gctaaaccaa aatttggaat agctggatca gggatgcact gtaacatgtc tttgtttgat
    10561 aaccaaggta ataatgcttt ttatgatgaa actgataagc gaggaatgca gctatcagaa
    10621 gatgcttatt atttcttggg aggactaatg aagcatgctt ataactacac tgctatcact
    10681 aaccctacag tgaattctta caaacgatta gttccgggtt atgaggcacc tgtttatgtc
    10741 gcttgggctg gaagtaatcg ttcaccactt atccgtgttc cagcatcacg tggtatggga
    10801 acgcgtttgg agttacgttc ggttgatccg acagctaatc cttatttagc cttggctgtt
    10861 ctcttggaag ctggattaga tggtatcatt aacaaaattg aagctccaga acccattgaa
    10921 gctaacattt ataccatgac aatggaagaa cgaaatgaag caggcattat tgatttgcca
    10981 tcaacgcttc ataatgcctt aaaagctctt caaaaagatg atgtggtaca aaaggcacta
    11041 ggttaccata tctacactaa tttcttagaa gcaaaacgaa ttgaatggtc ttcctatgca
    11101 acttttattt ctcaatggga aattgaccat tatattcata attattagac ttatcaggac
    11161 ctgctcaagt tgagtgggtc ttttggatga gctattgtat ttgagctagc aagctattat
    11221 tagctgatcg gacaccaatt gattttccat tatagaaaac taaaaaccag tccatgttct
    11281 atgaggactg gtttttagtt tgataattat ctgaactaga actgatatgt taaagatata
    11341 gacagatagt attaagaaat gagtcttatt tgattttagt gcttatcagg tgttaagacc
    11401 atcggaatta taattggttc acgctcagtc ttttcataca agaatggacg aagagcattg
    11461 acgatagcac cgttaactga ttggatgcta gcatccttat tttttaaagc aatacggata
    11521 gcgttaaaga gcacacgttg gctttcgcgg atcaagtcac ctgactcacg catataaatg
    11581 aaaccacggc tcagaatatc tggtccagct aaaatcattt gtgtgttaaa atcaacagtt
    11641 gcaacagcta agacaacacc atcttctgac aaatcacggc ggtcccgtaa aacggctgct
    11701 ccaatatccc cgatgccatt accatccacg tagatgtctt gggcattaaa gtgaccagca
    11761 atacgcgcac tgtcactggt taaagctaga acgtctccat tttccataat aaagatattt
    11821 tcttttggaa taccaatatc catcgccagt cctgcatgaa ctttttgcat gcgatactca
    11881 ccatgtactg gcatgaaata ttttggttta atcaaactaa gcatgagctt ttgttcttgc
    11941 tgtcctccat gaccagaagt atgaatatta ttgacttttc catgaataac atcaacacca
    12001 gcttcttgga tggtattgat taatttattg acacttgtgg tattaccagg gattggactt
    12061 gatgagaaaa tcacagtatc accaggctgt aaagttactt gacgatgagt gccattagcg
    12121 atacgagcaa gtgctgccat agactcacct tgactacctg tacacataat taaaacttca
    12181 ctagcatgaa gatttttaag ttcgcttggc tcaatgaagg taccttttgg aactttaata
    12241 tagccaagtt caataccgtt aacaattgct ttttccatag aacgaccaaa aacggcaatt
    12301 ttacgtcccg ttttaacagc agcttctgca gcctgttgca aacggtagat gtttgaagcg
    12361 aaggaagcaa aaataatacg cccatgaata ccttcgataa tttttaaaat ggattggcca
    12421 acaacttttt cagagttagt aaaggttggg atttcagcat tagttgagtc tgataataag
    12481 caaaggacac cttcttcacc aagcgctgcc atacgttgta aatctgcagg atctcctact
    12541 ggcgtgaagt caaatttaaa atcaccagta cagataatct taccttgagg ggtgtgaata
    12601 acaataccga ccggttctgg aatggaatga gtagtcttga agaaagtgac gctcatgttt
    12661 ttaaaagtta actcggtgtt gtgattgatt tcgtaaacag ttgcttcacg ccaaagacca
    12721 tgttcttcca atttaccacg gataagagca agtgctaaag gtcctgcgta gatagggata
    12781 ttagcctgtt tgagaagaaa tggaatacca ccaatgtggt cttcgtgacc atgtgtaata
    12841 acaagagctt tgacccggtc taagttatcg acaatgtaag aatagtctgg aataacgtaa
    12901 tcaattccca aaagatcatc ttctgggaat ttgataccgg catcaacgat aatgatctcg
    12961 tcttgatact caataccata agtattcttc ccaatctctc ccagaccacc aatggcaaac
    13021 accccaactt cattaggttt taaactgata tttgtcatct tagaactccg ttaattcaaa
    13081 aacaccggtc tctttttcgt aatcgaggtg tttgtcagaa agtagctcga taaattccac
    13141 gttataatag gtgttatctt caacgagacg acgagccttg atacggccat cgagttcatc
    13201 agtagcatca atattaagat agagtgcttt agtactttca cgacgtgggc tttgatcctt
    13261 tgtttcttga tagaaaactt tataaatcat ttgttttttt tcctttcgtt atgaatcaat
    13321 tatccaaaca ataataaata ggtcaaaagg taggtgttcc attttgacgg ttttatatgt
    13381 tgtcattatt gtgtagtaga ttaattcctg attctgtgca attattgcaa ttataccata
    13441 aacttagctt ttagtaaaaa atttaggtac atttatggta tagtctaaat caaattaaga
    13501 gtcagccttt ttgtttatga ttcttaattt gtgttataat tgatactaga acttgtagta
    13561 gaaaggcata aaatgaagac acttgcattt gatacctcaa ataaaacctt gtcccttgct
    13621 atacttgatg atgagacact tctagcagat atgaccctta acattcagaa aaatcatagt
    13681 gttagcctta tgcctgctat tgattttttg atgacttgta ctgatcttaa acctcaagat
    13741 ttagaaagaa tagtggttgc aaaaggccct ggatcttaca caggtttacg agtggcagtt
    13801 gctactgcaa aaacgttagc gtacagttta aatattgcat tggtcgggat ttcgagtcta
    13861 tatgctttgg ctgcgtctac ttgtaaacag tatccaaata ctttggtggt gccattgatt
    13921 gatgctagaa ggcaaaatgc gtatgtaggt tattatcggc aaggaaaatc agtgatgcca
    13981 caagcccatg ctccactaga agttattata gaacaattag tagaagaagg gcagctgatt
    14041 tttgttgggg agactgctcc ttttgctgag caaattcaaa agaaactacc tcaggcgata
    14101 ctacttccaa ccctgccttc tgcttacgaa tgtggtcttt tggggcaaag tttggcacca
    14161 gaaaatgtag acgcctttgt ccctcaatat ctcaagagag tggaagctga agaaaactgg
    14221 ctcaaagata atgagataaa agatgatagt cactacgtta agcgaatcta atatgaaaac
    14281 agttgaagaa caagcaaaaa atatttatca gcttttggaa atggtttatg gcacgtctcc
    14341 ttggacttta gaacaagtat taatcgatat atggcgggat caaacagatt atttcctact
    14401 atatgatcac gataaattgc taggtttttt agctattcaa gatttagctg gtgaagttga
    14461 aatgactcaa attgctatac tacctagtca tcaaggactt gggctagcgt ctcaattgat
    14521 gacgcattta ggtagcattg aaagtgacat ctttttagag gttagagaat ctaatcatag
    14581 agcacaaggt ttatatcaga agtttggttt taagtttatt ggcaaaagac cagactacta
    14641 tagaaatcct atagaaacag ctttattgat gaaacgggaa ggaaaaaatg acagataggt
    14701 atattttagc agttgagagt tcttgtgatg agaccagtgt agcgatttta aaaaacgaaa
    14761 gtaccctctt aagtaatgtc attgcaagtc aggttgagag ccacaaacgc tttggtggtg
    14821 tggtgccaga agtagctagt agacaccatg tcgaagtgat tacgacttgc tttgaggatg
    14881 ctttgcaaga agctggcatc agtgcttcag atttatcagc agttgcggta acttatggtc
    14941 ctggacttgt tggagccttg ttagttggat tagcagctgc gaaggctttt gcttgggcaa
    15001 atcatttacc tttgattccg gttaatcata tggcaggtca cttaatggca gcgcgtgaac
    15061 aaaagccttt agtttatcct ttaatagcac tgttggtttc aggaggacac acagaattgg
    15121 tttatgtccc agagccaggg gattatcata ttattggcga gacgcgtgac gacgctgttg
    15181 gtgaggctta tgataaagtt gggcgtgtga tgggcttaac ctatccggca ggacgtgaaa
    15241 ttgaccagtt ggctcataaa ggtcaagata cttatcattt ccctcgtgct atgataacag
    15301 aagaccactt agaattttct ttttcaggac tcaagtccgc ttttatcaat cttcatcata
    15361 atgctaagca aaaaggcgat gaactgattc tagaagattt gtgtgcatct tttcaagcag
    15421 ctgttctaga tattttattg gccaaaacaa aaaaagctct gagcagatac cctgcaaaga
    15481 tgcttgtagt ggctggtggt gtagcggcaa atcaaggatt acgagataga ttagcacaag
    15541 aaattacaca tatagaagtt gtgattccta aactaagact ctgtggagat aacgcgggta
    15601 tgattgcttt agcagcagct attgagtatg acaaacagca ttttgctaat atgagcctaa
    15661 acgcgaagcc tagtttagct tttgaccagt ttccagacag ttttgtaata aactagtcac
    15721 acttgctagt tagtaagatt tataatggat aagaatctcc ctttgaaaaa acaacccggt
    15781 atcttggcat accgggttgt catgatgata agtaattggc ttctatggtt actaataatt
    15841 cagataatta cctgcaaaat aattgtctgc ctaaggtgaa gtaagcttac cagctagctt
    15901 tagtgacgcc aggaagctgt cctttgtgag ctaagtcacg gaaattgata cggctaacac
    15961 caaacttacg catgtaagca tgaggacggc cgtcaatttt gtcgcggttc ttcaagcggt
    16021 ttggatttga atcacgaggc aatttgcgca gtgattcata atcgcccttc gcttttaagt
    16081 cacgacgaag gtcagcgtac tgttcaatca gttggagctg cttttggtat ttagcaattt
    16141 ttgacttttt tgccattgct ttctccttct taatatttaa taacctcaga gtcgttatca
    16201 gcgctctgaa tccacttaac tggtatttaa ctggttaagt ggatatataa tcattataca
    16261 ataaataaaa gcaaaagcaa ataaaaaaaa ttaactagtt atctttttta gccataatat
    16321 caaaaaactc cacatctaag tgtgggagtc aggtattaat aactgtttaa aggtgtttaa
    16381 tgagtgtcgc tttggcaact ttgagattga acttttaagt caaataaatc agcaggaacg
    16441 tagtgtttgg ttgagaccaa gatgtcttta ccattgtaga gagtttcttg gattaaaata
    16501 aaagcactat tttctgatag agtatactta gttgctgtgt aattgccagt cttggtatag
    16561 ccgatttggt tgcaggaatg agaagcttta ccagattcat agattttctc ttctaagaaa
    16621 gtaagaaggg gctcttcttg attgagattt atagcatatt tagaaataac ctcaataggg
    16681 ataaaagata aactataagc aatagcttta ttttggctat gataccagcg atcaacaata
    16741 atcacaacag gagtttcttg ttttagggat gctgtaatgg caacagtggg cacttcaatc
    16801 cgatattcca attcaacctt tgtgatgtca gatgataggc ttgcatatat ggggtgttga
    16861 agatattcga aaccttggtg atatttagtt tcaggagtct tgagtataaa atttcccttt
    16921 cctctgatat tttttatgag atgatcctct tggaggaggg ctagggattt acgtagtgtc
    16981 atccgactga cattgagctg tctagcaagt tcaggttctg atggcagttg catgccagga
    17041 ctatatgtac catcttgaat aagttgaaaa atagtattat atacttgcac atgtttaagt
    17101 tttttgagtt ttttggttaa gtcgttagta gacatatcac ttacctcatt taataatctt
    17161 tagtggagtt taacttgtct aaaaactatt ttagcactaa cagtatttct tgattagaat
    17221 tgtacccatc tatagtgtta gtatctcaca ctcaggtact ttttgtcaaa aaagagatga
    17281 aaaacacctt aaaaaagcgt ttacaaaata gagtgtgtgt gatactatac aagtgtatca
    17341 aaaattagac aagtaaaata caggaggttt ttaacatgca aaattattca ggtgaagtcg
    17401 gattgcaata tcacctacag attcgtccag gtgatgtagg tcgttatgtt attatgccag
    17461 gtgatccaaa acgttgtgca aaaattgcag aacattttga taatgcagtt cttgtagcag
    17521 acagccgtga atatgttacg tatacaggta cattaaacgg tgaaaaagtt agtgttactt
    17581 caactggtat tggtggtcca tcagcgtcta ttgcgatgga agaattgaaa ctctgtggcg
    17641 ctgatacctt tatccgtgtt ggaacatgtg gtggtattga ccttgatgtt aaaggcggag
    17701 acatcgttat tgcaactgga gctatccgta tggaaggtac cagtaaagaa tatgctccga
    17761 ttgaatttcc tgctgtagct gacttggaag tgacaaatgc tctagttaat gcagctaaaa
    17821 agcttggtta tacaagtcac gcaggagtag ttcaatgtaa agatgcattt tacgggcaac
    17881 atgagccaga acgtatgcca gttagttatg aattattaaa taaatgggaa gcatggaaac
    17941 gtctgggaac aaaagcgtct gaaatggaat ctgcagctct ttttgtagca gcaagtcatc
    18001 taggagttcg ttgtggatca gatttccttg ttgttggtaa tcaagaacgc aacgctttgg
    18061 gtatggataa cccaatggct catgatacag aagcagctat tcaagttgct gttgaagcac
    18121 tccgcacact tattgaaaat gataaatcac aataagcgag gttttggaag tcatcatgca
    18181 atttatttat agtattattg gtattttatt ggtattagga attgtgtatg caatttcttt
    18241 caatcgtaag agtgtttctc taagtttaat tggaaaagct cttatcgttc aattcattat
    18301 tgcgctaatc ttagtacgta tcccactagg ccaacaaatt gttagtgttg tttcaactgg
    18361 agttactagc gtaatcaact gtggtcaagc tggtttaaat tttgtgtttg ggtcattagc
    18421 agatagtggc gcaaaaactg gttttatttt cgctattcaa acgcttggta atattgtttt
    18481 cttatctgcc ctagttagtc tactttatta tgtgggaatc cttggatttg tagtaaaatg
    18541 gataggtaag ggcgttggta aaattatgaa atcctcagag gttgagagtt ttgttgccgt
    18601 agctaatatg tttcttggtc aaacagacag tccaattttg gttagcaaat acctaggtcg
    18661 tatgactgat agtgagataa tggttgtgtt ggtatcaggt atgggaagta tgtcagtttc
    18721 tattcttggt ggctacattg cattaggcat tccaatggaa tatctcttga ttgcttcaac
    18781 aatggttcct attggcagta ttctcattgc taaaatctta ttgcctcaaa cagaacctgt
    18841 tcaaaaaatt gatgacatta agatggataa taaaggtaat aatgctaatg tgattgatgc
    18901 aatcgctgag ggtgcaagca caggtgcaca aatggctttc tcaattggtg ctagtttgat
    18961 tgcctttgtt ggtttagttt ctttgattaa tatgatgtta agtggattgg gaatccgctt
    19021 agaacaaatc ttttcatatg tttttgctcc atttggtttt cttatgggat ttgaccacaa
    19081 aaacattctt ctagaaggaa accttcttgg aagtaagttg attttaaatg agtttgtttc
    19141 gttccaacaa ttgggtcacc taatcaaatc tttagattat cgtacagcat tggtagcaac
    19201 tatttcactc tgtggttttg ctaatttatc aagtttaggt atttgtgttt caggtattgc
    19261 tgttctttgc ccggagaaac gtagcaccct agctcgactt gttttccgtg caatgattgg
    19321 tggtattgct gtaagtatgc ttagcgcctt tatcgtcggt attgtaactc tattctaaac
    19381 gttgacaaaa gaaagaagga tagtaacgtg gaagtaaaag atattttaaa aacggtagac
    19441 catactttgc tagcaacaac agcaacgtgg ccagaaatcc aaacaatttt agatgatgcc
    19501 atggcttatg aaacagcttc agcatgtatt ccagcctctt acgtcaaaaa agcagcagaa
    19561 tacgtttcag gtaaattagc tatttgtact gttattgggt tcccaaatgg ctatagtaca
    19621 actgcagcga aggtttttga atgtgaagat gctattaaaa atggtgctga tgaaattgac
    19681 atggtcatta atttgacaga cgttaaaaat ggggattttg atactgttga agaagaaatt
    19741 cgtcaaatca aagctaaatg tcaagaccat atcttaaaag ttatcgttga gacatgtcaa
    19801 ttaactaaag aagaacttat cgaactttgt ggagttgtca cacgttcagg tgcagacttt
    19861 attaaaacct ctactggttt ttcgacagca ggtgctacat ttgaagatgt tgaagtgatg
    19921 gcaaaatatg tcggcgaagg tgttaaaatt aaggcagcag gtggaatctc atcattggaa
    19981 gatgctgaaa catttattgc tttaggagct tcacgcttgg gtactagccg tatcatcaag
    20041 attgttaaga acgaagctac aaaacccgat agctattaac ttaatcatac cgatttagct
    20101 tgagactaaa acaaaaaact catgtaatag gagttaaaaa gcctactatc ttagtgataa
    20161 taggcttttt tagctatgaa gacttccctg cgatccatcc tgaacaaaga gctgaggtaa
    20221 tattaaaccc tccggtgtga gcattaatat ctaagacctc tccagcgaaa tacaggccag
    20281 gaactttctt actttccaag gttttaggat tgatttcttt taaatccacg cctcctttgg
    20341 tcacaaaaga tttagccaaa gacatttttc ctgtaatggg tatctggagg tgtttgagtt
    20401 tatcaagcaa ttccttttct tgttttggag agagttgttt gaccttttcg gggtaatctt
    20461 ctgataaaaa atcagctact cgttcaggca gtaggccttt aagagcattt ttgatgtttt
    20521 tatcacgttg atcactcaga taagcggtta agtcatctgt tgataagtgt ggcaaaaagt
    20581 ccagctctgc aatttcccca cctttcacaa aagaggacag acgaagagct gcaggtcctg
    20641 aaaggccaaa gtgggtgaag agtaagtcgt gtgtgatgac gtgtttgtca taactaagag
    20701 taacgtcgtc tagggaaata ccttgcaaga ctttgtgagg aaaatcagtt aacagagggc
    20761 tctcagcagc ttctaaatca gtcactgtca atttaaaatg ccgtgcaata tcatgtccaa
    20821 aacctgttga acccgttgaa gggtaggact tgcctcctgt ggtcacaact aatttatgac
    20881 agctaaaagt ctggtctgca gatttgaggt agaagaggtc atcttgtttt ttaacggaaa
    20941 ccacttcagt gctggttagt accttgcctc caagagcctt gatttttttc tccagagcat
    21001 caatgattgt cctagattta tctgttgtgg ggaacatccg tccatggtct tcttctttga
    21061 gtctgacgcc gttttcctcg aaaaaggcaa tgatgtcatg gttgtcaaac tgggaaaaaa
    21121 cactatataa aaagcggcca tttccaggaa tgcctgccat aaggtcatca aggttaccac
    21181 tattagtgac attacagcgt ccaccaccgg ttcccgctaa ttttttgcct aagcggcgat
    21241 ttttttcaat taagagagtt ttatagccat agtagctgct ggagatggcg gccatcatac
    21301 cagcagggcc acctccaata ataatcgtat cgtattgagt catagcctta ttttagcata
    21361 aaagcggggg atttaatagt caggtaaatg aactaacctg gcataccgtt ttctattcat
    21421 ctaacaatgt tttcaagaaa taattgatgc ctttagcacg actgaaatga ggtcaaattg
    21481 acagaatact cctattatgg tactattttt aataagaata ttcagtgata gtgataaaga
    21541 ggaaaatatg ggaattgaaa agacagttag tgaactagct gatattttgg gagtgagtcg
    21601 tcaggccgtc aataatcgtg ttaagtcttt acccgaagaa gatcttgaca aaaatgaaaa
    21661 aggcgttact gtggttaagc gcagtggtct tgttaagctg gaagagatct ataaaaaaac
    21721 aatttttgac gatgagccta tcagtgaaga aacgaaacaa cgtgagcttt tagagattct
    21781 tgtggatgag aagaacactg aaatcacgcg cctttatgag caactcaaag ccaaagatgc
    21841 tcaacttgcc tcaaaagatg agcaaatgcg tgttaaagac gtgcagatcg ctgaaaaaga
    21901 taaacaattg gatcagcagc aacaattaac tgctaaagcc atggctgata aagaaaccct
    21961 aaaactagaa ttggaagaag ctaaagcaga agccaaccaa gctcgcttac aagtcgaaga
    22021 agttcaggct gaagttggcc ctaaaaaagg ctttttaacc cgcttgtttg cgaaataatc
    22081 acaaaaaatg acgaaggccc tatcagggtc ttttttggag gaaaacatga aacacttaga
    22141 tacttatatt gcctttgatt tagaatttaa cactgtcaat gacgttagtc atatcattca
    22201 agtatcagca gtcaaatatg accaccataa agaagttgac agctttgaca cctatgttta
    22261 cacagatgtt cccttacaga gctttatcaa tggcctaaca ggaatcacat ctgacaagat
    22321 tgctgcggca ccaaaagtag aggaggttat ggctgcgttt aaaaattttg tgggagagtt
    22381 accactgatt ggttataatg ctcaaaaatc tgacctgcct attctagctg aaaatggctt
    22441 ggatttaagg gaccaatacc agattgacct ctttgatgaa gcttacgata gacgtagcgc
    22501 agacttaaat gggattgcta accttcgtct gcaaacagtg gcaacctttt taggcattaa
    22561 gggtcgaggt cataatagct tagaagatgc cagaatgaca gcagcaatct ataagtcttt
    22621 tttagaaaca gatacaaaca aagcatatct cagccagcaa gaagaggtaa ccacagataa
    22681 ccctttcgca gctttgggag actttttcga ctaaaggctc tgctattttt gaaaaataaa
    22741 aaaatcccta gggttagccc ttgcttataa cgctaggtca tgtgataagc tagggacata
    22801 tcaagaggga gtcgagtgca cacgaatcgt ttttgatcac taaatgatag aaagaaggtg
    22861 tggaaatgtc tcaattctat tcgactggag aactggccaa cttagctggc gtttctattc
    22921 gaactgtcca atactatgac caacgtggga ttttgattcc gacagcattg acagcaggtg
    22981 gacggcgact atatacggat tctgatttag aacaattacg catgatttgc tttctgcgcg
    23041 atttgggatt ttccattgag cagattcgca aagtcttagc agaggaaaat gcagcccagg
    23101 tgctggaatt gcttctagtt gatcatattg cgactgccaa ggaagacttg gccgcaaaag
    23161 agcaacaagt tgacatagct gtcaagatac ttgacagact tcgaaaacaa gacccccagt
    23221 cactagattt tctgatggac atttcgctta gcatgaaaaa tcaaaaagct tggaaaaaat
    23281 tacaatggaa gatgggtatc agtatatttt tagccattgt ttcatatgta ggacttattt
    23341 tactatctaa ttatctgcaa aaattttggt tagtttatgt tgctatggga ctatttctac
    23401 caggtttcag ttggttggta atttatttta ggaagcaatt tatttatctc tgtccccatt
    23461 gtcatcagaa atttgaacct agctttaatg aatttgccct tgcaggccat acaccacgaa
    23521 ccagaaagtt aacctgctcc cattgccatc aaaaatcgta ttgcttggaa ctggcaaaag
    23581 aggacactgt ctcaagagga taagcactaa ctcataaaaa aactagcctg agatccttct
    23641 caagctagtt tttgtttttt tagaatggta atttaccagc aaaagcgcct aatgttttct
    23701 tagttgcttc atcaatttgc gtaagggcat cattaatggc ttgtgtggtc atgtcttgaa
    23761 gcgtttctac atcttcagga tctacaacag cttctttgaa gtcaatccca accaattttt
    23821 tatcacccgt gaaagtggca gtgacaaggt cttgagctga ttttccagta aattgcatag
    23881 cagccaaatc cgcctgcttt tgctccattt gtttctgaag cttttgtgcc tgcctcatca
    23941 tattctgcat attcatcata agactatact ctccttaatt taatactaac tattataaca
    24001 tataaaaaga ggcaataaaa acaatgtcag ttagtagact agcctgtatc agtaagaaag
    24061 tcgctatgga taagatggat tagcagcaag ggtctggcta atgacagaat aagtctagaa
    24121 atgaactatt tttgtcatag actagtccga cttagtggtt atctaattca cgatcttctt
    24181 tagcttgttg ttctaaaaga atttgtgcga agtcaaccca cttagtgtag ttattttcgg
    24241 ataattgttc tgaaatcatc gctaactctc gttgtggcaa ctgataagag actcccttac
    24301 tttctaaggt taaatcttct tccattaggg cggttaaact aacatcaaag atagcagcga
    24361 tatcacttaa tagaccagac tttggagtat attttccttt ttcccactca ctgattgaag
    24421 aagagctttt tcgtcctagg agattagcaa gttctaattg ttccatgttc ttttttaatc
    24481 taaggtactt taaattactt gcaaaaaaag ttttcttggc ggacatattt ttctcctttt
    24541 ctttatataa aatatgatac cataaaagag gttaaagcaa aaggtatcgt aaaagatggt
    24601 ctaaaaactc tctaaaaact tgacttcata aaaaccgaag tttataataa acttaacatg
    24661 attaactaat ctcattttag tgtggaggga aaattgtgac caaatcaact ttaaaagagt
    24721 taagaacaac aaagaaaatg acacagcagg aattgtctca cttaacaggt atttctgtca
    24781 gaacgattgc taggtatgaa aaagatacta aaaagctaag gcgagcaaaa tatgagaagc
    24841 taaaaggtat tgcacaagca ttagcggttt ccgttgatga tattttttta ggaatagatt
    24901 cggaatttat gaactgagac gatcgttttc tgtttgctat tagtaagacc aattaacaaa
    24961 aaagtgctta gttatattgc taagcacttt tctttgttaa tttgatgcta ttggcacaat
    25021 caattgagac tgactgagat caatagttaa agtatagttg ctgttgtcac ggacagtatg
    25081 ttcaaaatcg gttgtgtata agatgactcg aagagtattt cctttttcta gatgatagat
    25141 agttggttgc aaaggaagtc taacagtcat ccatttatta ttaggaatag tttcaattga
    25201 cgacaaatta ttgcgatttt gtaaattcat gaaacctttc gaaataacac ggtaagaatc
    25261 ttctctaaat ggtagctctt taaggggttc tctagagaaa ttttgcccat tgtcaatact
    25321 tgattgggtg agagcaatgg gaagatcacc caagcgtttt ttcttaccat agtcaaggat
    25381 ttgtgcagat agaagtcctt tattttcact tgatttcact tttaactgta ggacaatttc
    25441 accgttaata ggtaagtcct cttccagtaa aatatcaacc agggcttgat tggccttgcc
    25501 tttaaagaga gctgctttga acgcacggaa atctttaccg taagctttaa attcatcttc
    25561 accatagtga ttatcaatta aaagcagttc ctgaccgagg tcaagctctt tgatggttga
    25621 ggtttcaaag actttacgct cctgccagtt ttgtgggcag gtgttatcct gccagatcat
    25681 ttcaggaagg ctaaaatcat tagctaggcc taagagtttt tgacataaaa gggcattcat
    25741 gctttcgcga aaatcaatgg attgccaatt atgcatgtaa acatgctcac cttgatgtaa
    25801 aaagaggtgc ttgttgattg tggaaggaag cgcattgaca atctcataga cctgtcttgg
    25861 tttgacattc cagtcttgta agccatgagt ataaacgaca tcacacttta tttgatgagc
    25921 attttttaag taattgcggt cgtgccaaaa ttgattataa tcaccagatt gtcggtctaa
    25981 cgcttgagac tgttggttta gcaattcttg ataacgatca ttatggcgaa gataatcgcc
    26041 agccaataaa ttacgcgagt aagtgagctc agttaggaca tctagatcct ctccaggata
    26101 gccaccagga ctacaaacta agccattttc gcggtagtag ttgtaccaag aagaaatagc
    26161 tgattctgca ataatcatgg ctaatccgtc tacgcctgta gtggctaaac cagtagacat
    26221 agttcccaag taggactttc cagtcgtaca aactagacca tttgcccaat cagctctcac
    26281 ttggtgggtt tttgagtggc tggtgtaggc tgtggctctt ccatttagcc aatcaataac
    26341 ggctttaaag ctttcaattt gagcatagtc tccactcgtc ataaagccag tggaaccagc
    26401 agttcccaca cctgaaacat agatattagc aaacccacgt gcgaggaaat aatcgttgag
    26461 gctataggag ttaatgtagc taaagctttc tagattttga cctataggaa gtttgcaagg
    26521 ctgtgtctca agaggaataa aatccctgtc ttcgacagtg atttgtctcc tttttttaac
    26581 gactaactct ttttccatac gataaagttt tttgtcgttg gctacttcat tgataccttg
    26641 atggtatgga ctaggtgtca tcaaagttgg taatggtttt tgagattgag gacgaatgat
    26701 attagcctta ataagatcaa gttggccatc ttgatcggta tcaagaggtg cttcaacgta
    26761 aacaacttct cgaatcagct gtgacgtgtc aaaggtagct aatgatttac cgttgaagaa
    26821 atggtaatca ttgtccatag taagcattcc ttggctgacc aaatcatcaa tcagggtcat
    26881 gccagactta caacgggtcg ctaataagtg gtggatatgc ttgataattt ctgtttcgtc
    26941 aaagtggata ggaaaggcca gtttatcaag gaaagcatcg gcctctgtaa aatcaactcc
    27001 aggaataaag ccaaggactt gcagggccag taaatcaaaa acctctttgg ataactcttg
    27061 ctctgattga aaaaagctca atgcgtccat gtcatttttg gcgattagat gacttaaagg
    27121 ataatcacta tcagggtagt ggaagaagag tttacgtaaa aagctttcta ggttagcttt
    27181 ggcagttttt tgcagatcca gatcaaagcc caattctttt aattcttcag ccgctctttc
    27241 tagactagtt ggaatatagg aaaattgatt atagcgcata ggagtttcct ttctagtggc
    27301 ttagaaaacg atattatctt ttaatttatc aagattggta atgataatat gttgtttatc
    27361 aatgcgaatg atgttttttt ctttcaactg ttttagaata cggctgacgc tgctagcggt
    27421 agaaatgcca cagaatttac cgagttcctc attagtaatg acaaaattaa ttaaaatttg
    27481 gccatttggt agttcttcgc caaatagggt cattaagtca tatatttgag tagaaatggc
    27541 accgatacgg ccgttagtta acatacattg catttttttc atggattttt gaagacgatt
    27601 atggtaaaag tccttcacat atccttgtag ttcaatatct tcgtttatat cttttaaaaa
    27661 tgctgagcgg cgaaccttgt aaaaactagc tttctctgac tcgatgcgaa cattataagg
    27721 ctctcccata tcttttgaat aaccagtgtt gagaacagaa gtaatttcaa gtcctgtaac
    27781 atatctcaaa ttaaattcca ttccatattt tgaaagaaca ctctgtttca caatgccgtc
    27841 ttttaaaata taagtgaagt cctcttcgag actttcaaaa gtcaagtact tgtgataact
    27901 tttttcaatg ataggaaagt tatgattatc aatataccgt tgcaaaatgt gatgattcac
    27961 gcttttctcc aaatattctt ttaccaatgt ataatttgta tcttcaagag aaattaacat
    28021 ttgctaatgc cctttgatag taacacttat attataccat tgaaatatat tatattctgc
    28081 tttttttgta aaatgtttat gaaaatagag aggagttcat tttcaatgga agaaaaattg
    28141 tttaacaaac attttgtggc catcacagta ataaacttta tagtctatat ggtctactat
    28201 ctttttacgg ttattattgc ctttgtggcg actagagagc taggagctca gactagccag
    28261 gcaggattgg caacagggat ttatattcta gggactttat tagcccgttt gatttttgga
    28321 aagcagttgg aggtgtttgg tcgtcgtttg gttttaagag gaggtgctat tttttatctc
    28381 ttaacgacct tagcctactt ttatatgcca acgattagca tgatgtactt ggttcgtttc
    28441 ttgaacggat ttggttatgg tgtagtatca actgcgacaa ataccattgt aacagcctat
    28501 attccagctc gtaaaagagg agaaggaatt aatttttatg gcttgtcaac cagtctagca
    28561 gcagctattg gcccttttgt aggaaccttt atgttggata atcttcatat tgattttcgc
    28621 atgattattg tcttatgtag cgtcttgatt gggtgtgtgg ttgttggtgc ttttgctttt
    28681 ccagtcaaaa acatgtcttt aaatgcggag caattggcta aaaccaaatc gtggactgtg
    28741 gatagtttca ttgagaaaaa agccttgttt atcactgcaa tcgccttttt aatggggatt
    28801 gcctatgctt cagttttagg atttcaaaaa ttatacacgt ctgaaattca tttgacaaca
    28861 gtaggcgctt atttctttgt ggtctatgcc ttaatcatta ccatcactcg tccagcaatg
    28921 ggacgtttaa tggatgctaa aggcgataaa tgggtcttgt atccaagtta tcttttttta
    28981 gcaatgggac tcttcttgct aggtagtgta tcgtctggag gaagctattt gctttcagga
    29041 gccttgattg gctttggtta tggcactttt atgtcttgtg gtcaagccgc ctccatacaa
    29101 ggagtggatg agcatcgctt taatacggct atgtctactt atatgattgg tcttgattta
    29161 ggtttaggtg ctgggcctta cctcttgggc ttgattaaag atttggcact tggtagtggt
    29221 gtggcttcct ttaggcactt attctggtta gctgctgtga ttccactgat ttgtaccttc
    29281 ctttacttgt tgaaaacaaa gacaagacag gttgtgtctt aaggacaaga aaaggagttt
    29341 agtcttatac ttaaatacaa acgtatctta acagctgtag atagttccta tgagccggga
    29401 ttagctgtgg gtaaagccat tactgtagcg ctttgaaata atcctagttt gctattcgta
    29461 catgtcaata aaaatgactc gcaaaagcca ttcgtgaaag agaaaatgtg gccttatttt
    29521 tagtaggtgc aattggatta acatcagaat ataggatttg tcatgttaag attgatctgt
    29581 tgatagtgag agctaataaa aaatgttata atactatgaa ataaagtgat aaagataagg
    29641 tcttttatgg tccttttctt taccgctttt attattgtgt gaaaataaca cttaatgatc
    29701 attttattta ttattttatt acctttacat tcatcaataa gcctgttaaa atggtatttg
    29761 ttatatttta ttatttggag gaaaccagaa aacatggaaa tgacatggac tgtgaaatac
    29821 atcacagaat ttatcgcaac ggcttttttg attattttgg ggaatggagc tgttgctaac
    29881 gtcgacttaa aaggaacaaa aggacataac tcaggttggt tagtgattgc ttttggctat
    29941 ggcttaggag ttatgatgcc agctttgatg tttggtaatg tatctggaaa ccatattaat
    30001 cctgctttca cagttggact agcagtatca ggcttgttcc catgggctca cgtcttacaa
    30061 tacgttgtgg cacaattact tggagctatc tttggtcagt tggtagtggt aatggtttat
    30121 aaaccttact ttatgaaaac tgaaaatcca aatcatgttt taggctcatt ctcaacgatt
    30181 tcatctcttg ataatggtca aaaagacagc cataaagtct cttatatcaa tggtttctta
    30241 aacgaatttg taggttcatt tgttcttttc tttggtgctt tggctttgac taaaaactac
    30301 tttggggtag agttagttgg taaattgatt gaagcaggtt acgaccaaac aactgcagca
    30361 acacaaattt caccatacgt gacaggctca ttggcggttg ctcacattgg tattggtttc
    30421 ttagttatgg tcttggttac ttcattaggt ggaccaactg ggccagcact aaacccagcg
    30481 cgtgattttg ggcctcgttt gttacaccac ttcttaccaa aatcagttct tggtcaagcc
    30541 aaaggtgatt caaaatggtg gtatgcatgg gttcctgtag ttgcacctat cttagcggcg
    30601 atagtagccg tagcagcgtt taaatacctt tatatcagat aaaaaaagaa ctaaagcttc
    30661 ttttagactc aagacaagct cgcttaagga acttgtcttt tttatatgag ttaagcgaaa
    30721 tctaatgcaa acttgactgt cttgtatagc gtattttgta aattttacta gacttcttgt
    30781 aaaaaagcct ctcctcagat aagatgacta atgcctctaa acggtgttac aaataaacga
    30841 ggcatcccta acttccttta tctggtgttg aaactatttt gcacggacta aatcactgat
    30901 agagtaaaaa acgatttttc atttttgttg taaaatatta aaaacgtctc tggttagtga
    30961 ttcccagaaa cgttatctta tctatttgtt tgttgcttat gagaggtaaa ggaggaacga
    31021 atcagcattc caccagtgaa gaaaacgata gcgatgacca gaagtttcaa gctattaata
    31081 tctaaattcg ttaagaaaaa cacagctagt aaaacgccaa taataccacc agaaacaatt
    31141 ccagcaaaac catcccaact gacacggttt gctttgataa actggctact agaagctgtc
    31201 ataatcatag ctgcatctag catcataaca ggcatagcaa ctgttgggct aagacccatt
    31261 aatgaaaaga aaatcaattc tggagcataa ttgcctaacc ccatagtcat caagactcca
    31321 acgataaaat taaaaccaat tccaacaaaa agccagatgc catggaggcc atgaatggtg
    31381 tctgaaaggt gttctcctgg atggtagatt attctaatga tcatgattat agctgctatg
    31441 attaaaagac taccaaggat tcgttggaca gttggagcat gccagttttt tgtaatatga
    31501 gtcccaaaat aagctccaat aaaggctgct gcagccatag caagtaaagt tagaacctca
    31561 acttttacga caaaaataaa gcaaagagat tgaatgagca ccggaagcac atgagctacg
    31621 gtcatagtgc caggcaattg tctatcgtca gtgactaatt tagttaactt aaagcacgtt
    31681 gtggtagtag caaaagaccc tatcccaagg gtgtcaaggg catctgttag caggccaatc
    31741 caaaaccccg tccaaaagcg tttaaaagga ttgatcttat tttttttaag atgttttact
    31801 aacataaaga aaataaaaag cattgccgaa actagcaaag cttgtatcag tctaaggata
    31861 agcatttcgt tcataaagac tagtttacaa aaaaaaacct tagccgtcaa cgtgatttac
    31921 ttcattaaaa catgccacta tgcttagaag agctttagtt cgatgtattt tgttaaaatt
    31981 aagctaaaat attaatattg catgaaaggg gacgccttcc tttttccctt actgagctta
    32041 gctgttattg tagtgaatgt ttaaaaggct agtttgtcgc tctttatcaa aaatctagta
    32101 ggatgaatac gtataatttc ttgtcaaata gaggaatttt gtctataata gcagaggatt
    32161 aggaggaaat tatgacttta atggaaaata ttacctttaa aactccagta ttacgcgtta
    32221 atgatcgcga ccttaatatt gcattttatc aaaataattt agggcttcgt ttagtatctg
    32281 aagaaaatgc gattgctatc ttttcatctt ggggagaagg gcaggaatgt tttgtaattg
    32341 aagagtcacc ttcagttaga acgcgtgcgg tagaaggccc taaaaaggtt aatacgattg
    32401 tcatcaaaac aaatcaacct aaagaaatcg aacaattatt agctcatgga gcacattatg
    32461 acgctctttt taaaggtcaa aatggctatg cctttgagac tatttcccca gaaggagacc
    32521 gattcttgtt gcatgctgaa caagatatta aacatctcca aggtactgat ttaccatcgc
    32581 ttgaaaaaga tgcgactttt aaaggtttaa ctcaatttaa gtttgatatt attgttttaa
    32641 atgttattag tgaagaacgc tcaaaagctt tttatcggga tctgtttagt gatcagttac
    32701 ctattactat ggattttatt caagaagaag gtcccgattt ggcaattgat ccccatattg
    32761 cttgggattt agaaatttta gaatttcaag tctcaaagga ctatgatatg aaggtcctaa
    32821 aagcaacttt agaagaagac gggcacaagg tctacattga taaaaaacac aaagtattgg
    32881 tcctttctga tccaagtcag atcgaagtat ggtttacgaa atgactttgg cagtcattaa
    32941 atgtattgaa aaccatcttc acaaaaaagt atacaaaggg gccagtctgg ctctctttca
    33001 atctggtcga tggcaagaat accatatcgg aacaattgat ggaagacgac cagttgatgc
    33061 taacttagtt tatgacttgg ctagtgtttc aaaagttgtt ggtgtggcca caatatgcaa
    33121 tattttattg aataatggta cgttagcatt agatgatccc ttaaaagtat attatcctag
    33181 tattgctgat acgactgtca ctattcgaca gttattgact cataccagtg gtcttgatcc
    33241 ttatattcct aacagagatg ttttaaatgc acaacaatta agaaaagtac ttaatcatct
    33301 tactcaaaaa gaaaataaaa acttttatta cacagatgtt aatttcctct tgttaggctt
    33361 tatgttagaa gagctcttta gtgaatcttt agaccagata tttgataaga ctatctttac
    33421 tccctttggg atgtatcata cgtcttttgg ccctcgtcca gaagctgtac caacattaaa
    33481 gggtgtgagt gatggagaag ttcatgatcc caaagcaaaa atcttaaaaa aacattctgg
    33541 atctgcgggg ctattttcaa ctcttgcaga tttagaaagt tttagcaatc attatcttaa
    33601 tgatccattt tcagattgtc tgtggcgcaa ctacagccaa caaacaattg agcggtcact
    33661 agggtggaat ttagatggcg attggattag tcatacaggt tacacagggc cgtttctaat
    33721 gcttaataaa aaagagcaaa cggcagctat ttttttgacc aaccgtactt acgacgagga
    33781 tgataaaagt aaatggttaa aagaacgtca gttgctttac aatgcactaa agcatgatct
    33841 gacaacaccc gtatcgtaac gtctaaaggg gcagttttag tgatcttatt actgttttta
    33901 aagtttcttc taaaaaatag ccatttcatt tgtctgatgc gattagagtg attataagtg
    33961 aaaaaagaac agccatctca ttttgaatca aatgagatgg ctgttctttt ttataagtta
    34021 ttgatcattt agatactact atttacttga aatagcttca gcggcatcat ccattgaaag
    34081 cacttcgtgg aagacacgtt gagtcaattc agttttttgt tctggtgtaa ggtatttggt
    34141 gttgacacag taaccagaga tacgcacgat aacgtcttcg ccgttcatga ttttgtcata
    34201 aacatcgtta aggtccataa cgttcaagtt aacgtgctga ccaccatttt caaagtaacc
    34261 gtcaagcact gttaccaagt tgtcaacttg ttcgtcaaat gttttaccaa gagcacgagg
    34321 tgatacttga gtagtcaatg agataccatc gttagcatgt gagaattcca gtttagcaag
    34381 tgagttaagg ttttgtaacc agccaccttt tgctttgtta gatgggttag cacctggtga
    34441 gaagaattcc acttggctag tgttaactgt accatcttcg ttcaagaaga cgccgcggtg
    34501 aactggagag ttaccagttt gtttagagta agcaacgttt gaagtgatgg taagaagtga
    34561 tactgaagcc tcagcattct tgtaaagttt gtggcttgca agacgtgtgt ggtaagcttc
    34621 catcaaccat ttcgcgatgt catcaacacg gtcgtcatct tcaccgtaac gagggaagtc
    34681 acctgttact tcgtagtcat aaatgtagcc attttcatcg cggatagttt taacagttgc
    34741 gtatttaata gctgacaagg tatcaactgt gttagcgaaa ccacagatac cgaatcccat
    34801 gttagcacgt tggtgtgttg gcaagaaggc catttgaact gcttcgtagt tgtatttatc
    34861 agtcatgtag tggatgatgt taagcgcatc tacataagta tctgtcaacc agtcaagaga
    34921 tttctcaaag ttagccataa cttcgtcgta ctcaaggact tctgatgtga ttggttcaac
    34981 aacgttaaac actttgtagt ctctgtgaac atcatcgtaa ccaccgttaa gaccagtaag
    35041 aagggctttg agaacgttta cacgagcacc gaagtattgg atgttatgac gttgttcttc
    35101 attttctggg tcaagtggtg atacacaaca tgaaatacat gacatttcac cataaccttc
    35161 tttagccatt gttgtcacac cttcatattg gatagaagag tgtttgtggc tcattttcat
    35221 acagtagcga cggaatgttt ctggcagttg gtcagtccaa agaactgtta agtttggttc
    35281 tggagagtta ccgatattat caagagtgtt taagaaacgg tagtccatct tagtcacacg
    35341 gtgacgaccg tcgttaccca taccagccat agaagttgtg atgaatgttg ggtcacctga
    35401 gtaaagggca tcgtaagctt tcgtacgacc aaattttacg gtacggagtt taaggacaaa
    35461 atcatccaca aattcttgga tttctgattc tgtaaaggta ccgcgagcaa ggtcacgttc
    35521 tgcaaagata tcaagaacga ttggcacacg tccaagagaa gtagcagcac cgttgatcac
    35581 acgacataca gccatgaaag cgatgtttac ccattggata gcttctttaa cgttttgtgc
    35641 tgggcgacga acatcaactc cgtaaaggtc accaagttta accacttcac caagagcttg
    35701 gtattgaagg ttaacttctt cgcgaagacg aatggattct tcatcgattt cagtgatagc
    35761 attccagtca ttgacttttt cttgcatcaa gtagtcagca ccgtaaagag caagacgagc
    35821 gtaaacaccg atgatacgtc cacgtgagta agcatctgga agaccagtta cagtgtgggc
    35881 atgacgagcg cgacgaatat ttgaagtata agcgcggaag ataccgtcgt taacagttgt
    35941 cacatactta gtaaagattt catgtactgc tgggtctggt tcatagccgt tttctttaag
    36001 ggtcgtttcc gccatacgga taccaccttt tggcatgaag ttcaatttga acaattcatc
    36061 gttttgaata ccataaatta attcattttc tttatcgatg aaaccagcag gaatgtcagc
    36121 aattgaagtt ggacgagtat cgtatgggaa acgtgtagct tcatagtgtg cttttgtttc
    36181 ttcgataact tttttgatgt gaagtgaacg ttcagtagca ccagccaaga aagattcatc
    36241 gccatcgtat ggggtgtagt tagcttgtac aaaacgtgag acacttgctt tttctttcca
    36301 gtcagttcct ttaaagcctt cccaagcttt ttcgaaaaca tctgtattgg ttttaacagt
    36361 tgccataaat ttctccttaa attctagtaa caaagcgatc tgttacattt acaattctaa
    36421 gtttaacaca taggaaacgt tttcccaagc aaaattcttg acaaaattaa gattctttta
    36481 taatacagat tgttgaaaca gtcacaaact gtattagaat gtattttttt ggctttaggt
    36541 agcctaaaag gtataataga gaaaggaggg acttatgctt atttttccac tgattaatga
    36601 cacgtcacga aaaatcatcc atattgatat ggatgccttt tttgctgcag ttgaggaaag
    36661 ggataaccct gctttaaaag gaaagcctgt tgtgattggg aaagatccaa gagaaacagg
    36721 tggtcgcgga gttgtttcca cttgtaatta cgaagcgaga aaatatggca ttcattcggc
    36781 catgagctct aaggaagctt atgagcgttg tcccaaagcc atttttattt caggaaatta
    36841 tgaaaagtat cgaacagttg gggaccagat ccgccgtatt tttaagcgtt atactgattt
    36901 ggttgaacct atgtctattg acgaggctta ccttgatgtg actgataata agttggggat
    36961 taagtcagcc gtcaaaatag ccaagctgat tcagcatgat atctggaaag aagtaggatt
    37021 gacctgttca gcaggtgtgt cttataacaa atttttggct aaattggcta gtgattttga
    37081 aaaaccccat ggcctcaccc tagtcttgaa agaagatgcc ctgtgctttt tagccaaact
    37141 ccccattgaa aagtttcatg gtgttggtaa aaaatcagtt gaaaaactgc atgacatggg
    37201 gatttataca ggacaggatt tgttggcagt tcctgaaatg accttgattg atcattttgg
    37261 tcggtttggt tttgaccttt accgtaaagt gagaggcatc agcaattctc ctgtcaagtc
    37321 tgatcggata cgcaagtcaa ttggcagtga gagaacctac gctaaactgc tttatcaaga
    37381 aacagacatc aaggcagaga tcagtaaaaa tgctaagcgc gtggccgctc tcttacaaga
    37441 ccataaaaag ttaggcaaga ccattgtgct caaagtgcgt tatgctgatt ttaccacctt
    37501 gacaaaacgt gtcaccttgc cagaattaac cagaaatgcc gcacaaattg agcaagtagc
    37561 tggggatatt tttgacagct taagcgaaaa tcctgctggt atccgcctgt taggggtaac
    37621 catgaccaat ttggaggata aggtagctga tatttccttg gacctatagg agaaagaggg
    37681 ctctatccgt ccgcctcatt ttggagaaaa aagaagctga ttagggattt ccctaatcag
    37741 cttctttggt ccattatttt attacagcat agcttggtct gtatcatcac ctttttcgtt
    37801 aagggtttta gccttgtaaa aagcaaagcc ataaagagca gcttgtataa ggataaggat
    37861 agttccaacc attgacaagg ttgttgtgaa ctggttaacc aagccgataa cagaaaggat
    37921 aagtcctaag atataaggaa tataactaac cgttaatcct tgttttagtt tggataagtt
    37981 ttgagcacaa aagacaatga tagcaataat agcaaggaca tttaagacag ataagaaaat
    38041 cataaatggt gtcatgctgc tttgtagctg tgccaattgt tcagttgtta attgggcccg
    38101 aaatgcttca ttcttgagtt ggctttgtaa gtagaaaatc cctgcaaatc ccattaatga
    38161 cagtacaact ccaagcccgt ttagaataat aatggcaata gtagacgttt tgagtgcctg
    38221 acgcactttt tcataagaaa tcatataatc ctcctagagt ccttaatccg aaattgtaca
    38281 atgtgcttaa tacaaaaata ttataccata gattaatcaa aaatgtccgt ttttctagga
    38341 ttttttctgg aaaaagaggg caatatctga ctctgtaagc cctatcatcg aatcgatagt
    38401 cgaataattc tcaggtgtta acctaaaatc atcattatct gactgagagt tctcagtggg
    38461 gttagagttt gttgattgag aggaagattt ttgagagtcg tcctcaaggg ttgccgtatt
    38521 agaaaaaagg gaggtctgct cttttgattt tagctcttgg ttaggttgag aggaggctaa
    38581 gtcggattgt tcttggaagc gttcgatcaa gtaggtttgg cgtttatcgc cttcgtgttt
    38641 aatagcatac tcaaaggcag tatattctcc caacagaatt aacttacttt tagaccgagt
    38701 aatggccgtg taaatcacat ttcgttgcaa gagtcggccg ctttggcgtg tgataggtaa
    38761 aatcaccgct tgaaactcac tcccttgcga tttatgaatg ctcatggcat aggccagggt
    38821 taattttagc cattcatttc tagggtacgt gacttcgcta ccgtcaaaat ctaagattaa
    38881 ttcgtcttgc ttagattcgg tgtatttagc aggaatcaaa tctgtaatat aaccaatatc
    38941 tccgttaaag acattcaact gagcatcgtt gactaagtgc aggactttat cacctttacg
    39001 aaaatacgta tcattgaaca gaaattctgt ttgtccttgc aagggattga gaagttcttg
    39061 catgagttga ttaagatgat tgatgccagc ttgtcctttg tacataggcg ctaaaatttg
    39121 aatttcctca gcagggatgc cacttttaat agcagatagg acaatttttt gaatcatgtc
    39181 agggataaag gctgcttggg cttcaaaata agaacggtca gcttttttat cacgaaagtc
    39241 agcagctaag attcctcgac gcatctgatc tgccaaattg acaatagtag attcttggga
    39301 ttgacgaaat atcttttgca aggcgatttg gggcaggcta ttgactttta aaagatctga
    39361 caggacttga ccgggtccaa ccgaaggaag ctggtcacta tctccgacaa taatcacttg
    39421 agttgttgag ttaatagctc ctaacaactg gttggcaagc caggtatcta ccattgaaaa
    39481 ttcatcgaca atcagcaagt cgcagtctag ataatcctcc atggcttggt aatcgttgtc
    39541 gccattgagg cctaagtgcc tgtggatggt tgcgctagga agtccagtca actcattcat
    39601 gcgacgtgca gctcgacctg ttggagctgc taacaagata ggaaggtctt ttttatccaa
    39661 atcaatctga tgcaggttcg cgtaagcctg caaaatgcct cgaataacag ttgtttttcc
    39721 tgttccggga ccacctgtta ggagaaagac cttgctggtt aaggccttag taatggcttc
    39781 ttgctgcact tggtcatagg tgatggcaaa gtctttttga acggcttgga tggttgtttg
    39841 aatggtatca tgactaaatg actgacttaa aggggtatct aagagacgag agatgttgtt
    39901 agcaatccct tcttcagcaa aataaaggct agcatcaaac agtttggtgt cactgttttt
    39961 gattttgccc tctatgatca actcgcttag ttgttcagcc acagcagcag gatcacattc
    40021 tacttgacgt gcatcttcaa ggagtgtgat ggcaaagtct aataattgtc gtgcttgaac
    40081 ataagtatct cctcgattga tggattcttc caagagacaa tgaaggaggg ctgcacgaaa
    40141 acgtttcgga gagtcgcttt caatccctaa gttttcagcg agagcatctg ctattttaaa
    40201 cccaaaacct tgtagatctt cgactaactg atagggattt tctttaacaa ggtcaagagc
    40261 ctcttcttta tacttttcaa acgcttgaaa ggcaaaacgg ttgctaaggc caagttcgac
    40321 gagaccagca atcagttgct ctgtgccgta attcaatttt agtttagcga caaaggcttg
    40381 acgattagcc ttagataggc cagagatagt ttctaattta cttgggtctt ctaaaatatg
    40441 gtcaatagta ttatgaccat atagtgcaat gattttctcc gctgtttttt taccaatccc
    40501 cttaaaatgg tcactagaga aataattaac caaaccagat gaactaggtt tgattttttg
    40561 gtaacggctt agcttgagtt gttgaccgta tttagggtgc tgggtcaatt ctccccaaaa
    40621 ggtgtagtca tctccttcaa taatgtcagc catcgttcct gtgataatga tttcaaagtc
    40681 gtcaatgtca ctgtctgtat cctcaatggc aaggaggaga attttaaaga aattagcctg
    40741 gttttcaaaa atgatacgat caacagtacc tgtaaaaacg tattccatag taactctttc
    40801 tatttataca agataactga aaacctgccc aaaggcaggt tttttctaat taaagacggt
    40861 cattttattg agtggccaaa aacgagcttt cacttcaccg ataaggtttt cttttttgaa
    40921 actaccaact tcgcgactat cccgggaaac aatacgatca tcaccaagaa gaaggtattc
    40981 tccttttgga acactcatct caaagcgtgt ttgtccctca gaattagttg taaaagcatc
    41041 agatgtttct gctaactgtt ggaatagggt attataggcg taagtttttt ggagtttatc
    41101 gtttttaaat tgttttagat actcagccaa atacggctca actgtttttt taccattaat
    41161 ataaagtgtg tcatcattat aagaaatggt atcgcctggc aatccaataa ctctttttac
    41221 gatttctttc ttttgtccgt tttcttcttc ctgagcaact acaatatcaa agcgatcaat
    41281 cctagcttga ttaaaaacga taaggcgttc gccatgagct agagttgggt ccatagaatg
    41341 gccgtctact ttaacagcct gccacaaaaa aagacgagat agaccaaaga gaattaaaaa
    41401 gagagtgaat gggccccatt ctttaataaa ctgtttcata tgattccttt tatttagtga
    41461 gaagcgcttg cgctttgtga gtgttggcga agtgaagttt agcactatat tctaagctag
    41521 acataccata agcggctaag agttggctag caactttatc tgaagctgaa ccagcaccgc
    41581 ttgggagttg gtaacctaaa tcttgaccta attgatcgag attgtctaaa aatagatttc
    41641 tagcaatgat agagctaaca gccacagcca aatagtgact ttctgccttt tcttgaaagg
    41701 ttagaggatt cgggaaatgg tttttttcct tttttagatg cttttcataa tttgattggc
    41761 tagtgaaagc atcaataaca atttgtttag gttgaatgcc tttttgtaac aacaaaaaaa
    41821 tagcttggtt atgaagagca actttgatag aaatggcatt atatggttta cttttaccaa
    41881 ccaattcgtt atatttttta ggtgatagga gaagtgattg atgaggtatt tgtttctcta
    41941 acagaggcgc aatctgttga atggttttat ccgataattt tttagaatca tcaaccccaa
    42001 gctttttcag gaagggatga tctttaggat cgacaaaact tgccacaaca gcaatcccac
    42061 caaaataaga gccattaccc acctcatcac tgccgattat aggtatatct tggctattgt
    42121 tgcttgcttc aaaaactgtc ttagctactg gaagattaag ctcttgagct aaagcatttg
    42181 caccgtttcc ctggagaact aattttccgg atttgtacag caaaacagta accccatttt
    42241 ttttagcagc aaaggctaca taggtatttt ggctactgat ggtgtagggg gctagctgtt
    42301 tttttaaatg tttactcagt atagcatcta ttttcaaaac taaggtattc ataataatta
    42361 ttttaacata aaatagttga ctatggttaa ttcataagtt tatttaacaa agaaaacaga
    42421 gactcgaatg tttagacggg ttagggcaat ggtgttgact attttacgaa agtttcattt
    42481 tacggtataa tgctaatgag gtgaccctat gaaaagtatt aatcgctata aattcacctt
    42541 tggtgaaaaa acattaacat tgacaactga caaagacaat ctctttatgg aagaagttga
    42601 gcgggtagct aaagaaaagt atcaggctct taaaaatcat ttaccagaag ctgatgacga
    42661 gacaatcgct attttaatgg ctatcaatac cttgtcaaca caattaagca gagagattgc
    42721 cattgaaaaa atggaagctg aaattttaga tttacgtcaa aaaaccttgg ttggtcttca
    42781 agaaaaagct aatcaaatag atgtgaatga ggtttaatca tgttatcttt attgatagtg
    42841 cttgttttaa cttggaattt ttatattggt tatagtagag gtattattct tcaatcgttt
    42901 tatgtcttag gagccctact ttctcttttg gtagctaatc gattttatat tggattagcc
    42961 cataagctaa cgctttggat tccttattct aacccggtag aaggaacatc agtctttttc
    43021 tttaaatcag ttgatatctt tgttttagat aaggtttatt atgcaggcct tgcctttttt
    43081 atcattttct tattggggta tgcactgagt cgctttcttg ggatttttgt tcatttcctt
    43141 ttgctagatt attttgataa ccagtggaca aaatgtttga gtggcggact agcgcttcta
    43201 gtatcgcttc tttttttgaa catgttactg tctatttttg caacggtccc tatgcctttt
    43261 ttacagcact atttgcactc tagttttctt gctcgtttgg tgattgaaca ccttccacca
    43321 ttgacaataa ttattcaaaa attgtggatt caagccatta tttgatatcg ttttgtgact
    43381 aatataagaa cctggagtga gtttcacctt taggttcttt atttacgaga aactgtttag
    43441 aaataaagag aacgcatggg aacttagtga ctttcttgcc acattttcca tcattttagg
    43501 tataatgaag agtatgaata acaagatttt agagcagtta gaatttaaca aagttaagga
    43561 attgctatta ccttatctca agacagaaca atcccaagaa gaattatcag agctggagcc
    43621 gatgacggag gctcctaaaa tagaaaaaag ttttaatgaa atttctgaca tggaacagat
    43681 ttttgttgaa catcactcat ttggcatagt cagcctaagt tcaatctctg agagtttaaa
    43741 acgcttagag ctttcagctg atcttaatat tcaagaactt ttggctatca aaaaagtttt
    43801 acagagttct tcggatatga ttcactttta ttctgatttg gataatattt ctttccaatc
    43861 tttggatcgt ttgtttgaaa atttggaaca attccctaat ctgcaagggt cttttcaagc
    43921 tatcaatgat ggtggttttt tagaacattt tgcgagtcca gagttagagc gtatccgtcg
    43981 tcaattaaca aatagtgaac gacgggttcg tcagatttta caggatatgc ttaaggaaaa
    44041 agcagagctt ttatcagaaa atctaatcgc tagtcgaagt ggacgaagtg tcctaccagt
    44101 aaaaaatact tatcggaatc gtatttctgg tgtggttcat gacatctctt cttcaggaag
    44161 tactgtttat attgagcctc gtgctgtagt tacactaaac gaagagataa cgcagcttag
    44221 agctgacgaa cgccacgaag aaggtcgtat tttacacgca ttttcagact tgttaagacc
    44281 tcatgtcgcc actattagaa ataatgcatg gattcttggg catcttgatt ttgtaagggc
    44341 taaatatctt tttatgtctg ataataaggc gacgatacct aagatttcta atgacagcac
    44401 gttagcatta atcaatgttc gtcatcctct gttaagtaat cctgtggcta atgacttaca
    44461 ttttgatcac gatttaactg caattgtcat cactggtccc aatactggtg gtaagacgat
    44521 tatgctaaaa acactcggtt tagcacaatt aatgggacag tctggtttgc caatattagc
    44581 ggataaaggt agtaaaattg cagtatttaa caatatcttt gcagatattg gcgatgagca
    44641 atctattgaa caaaatctat caactttttc tagtcatatg acgcacatag tcagtatttt
    44701 aaacgaggct gaccacaata gtttagttct ctttgatgaa ctgggagcag gaacggatcc
    44761 tcaagaaggt gctagtttgg ctatggctat tttagaacac cttaggttaa gtcatatcaa
    44821 aacgatggcg accacgcact atccagaatt aaaagcttat gggattgaaa caaattttgt
    44881 agagaatgcg agcatggaat ttgatgcaga aacgcttagc cctacgtatc gctttatgca
    44941 aggagttcct ggacgctcaa atgcatttga aattgcttct cgccttggtt tagctccatt
    45001 tattgttaaa caagctaagc agatgacaga ttctgattca gatgttaacc gtattattga
    45061 acagttagag gcacagacac ttgagacacg tagaagactg gatcatatta aagaagttga
    45121 acaagaaaac ctcaaattca atcgtgcggt taagaaactc tataatgaat tttcacatga
    45181 acgcgataaa gagttagaaa aaatctatca agaagctcaa gaaattgtag atatggcttt
    45241 gaatgagagt gatactatct taaaaaaact caatgataag agtcaattaa aacctcacga
    45301 aattatagat gctaaggcac aaataaaaaa attagcgcct caagttgatt tatcaaaaaa
    45361 taaagtctta aataaggcta aaaaaatcaa agcagctcgt gctcctagaa ttggtgatga
    45421 tattatagtg actagttatg gacagcgagg taccttaact agtcaattaa aagatggccg
    45481 ttgggaagca caagtgggaa ttatcaaaat gacattaaca caagatgaat ttagcctcgt
    45541 tagagttcaa gaagaacaga aagtcaaaaa taaacagatt aacgtggtta aaaaggctga
    45601 tagttctgga ccaagagctc gacttgatct tagaggtaaa agatacgaag aagcaatgca
    45661 agagttagat cattttattg atcaagcatt gcttaacaat atgggacaag ttgatatcat
    45721 tcatggtatt ggtacaggcg ttattcgtga gggagtgaca aaatatcttc gtcgtcataa
    45781 gcatgttaag cattttgctt atgccccaca aaatgcaggg ggatctggtg ccacaattgt
    45841 aacgttaggg taaatgatgg aatcatatga acacttttat gccaaactat ctcaaccttt
    45901 tagaaaaacc ccacagttaa taattctttt aaatgtttta ctaaaaataa tcacaggagt
    45961 gatgtacata ctttatccat cttttctgat atttacgctt tggcaaggga tgacatttca
    46021 attatggtta aggctattaa ttattcctgc tgttggcttt atagctttgt cttacattag
    46081 gaaacgcttt gattttccac gaccgtatga aaaatggaat attaaacctt taattgataa
    46141 ggatacaaag ggaaggtcaa tgcctagcag acatgttttt tcagctacga tgattagtat
    46201 gtgtttgtta cgatattatg tttactttgg aatagtttgt ttaatattat cggttttatt
    46261 ggcaatttgc cgtgtgatag caggtattca ctatcctaag gatgttattg taggttacct
    46321 tattggcttg atgttagggc tttgtttatt gatttagcga gttcgtagca aatagtttca
    46381 aaaacaacta aattcttgta caataggctt atcattaaga tgaaatggag aaaaaagatg
    46441 gcattagaag taacagatgc tacatttgta gaagaaacta aagaaggttt agtactcatt
    46501 gatttttggg caacttggtg tggtccatgt cgtatgcaaa caccaatctt agaacagtta
    46561 tctcaagaaa tcgatgaaga tgaactcaaa attttgaaaa tggatgttga tgaaaatccg
    46621 gaaactgctc gtcagtttgg tattatgtca attccaacat tgatgtttaa aaaagacgga
    46681 gaagtggtta aacaagttgc aggtgttcat actaaagacc aactgaaagc tattattgct
    46741 gaattgagtt aactaaaata aagcccagcg tcacgctgag ctttatttta tgacctacta
    46801 acttcaatgg aataaccatg taaacataaa acgacttgca aaaaagatgg caacagtaat
    46861 gaggaaaaat acagtgacaa taagggggtt ctgataagga agttttgctc tttcttctcg
    46921 tttttcgatt tcgtctaata attttccact tgaaacagct aggatttcct tataaggttg
    46981 cacatcatat tttttcttaa aacgttccac acgattagca taagtcatag taattatcga
    47041 taataaaaca aaaatgccaa gtcctagcca ctctaagaaa tagactaatg gataacttga
    47101 aatcattaat aatatcatca atgttaccat tactttggta tctcgtttaa aattcttttt
    47161 atcaacctga gtgatggtat atttcatttt ctcaatgtct cctttaatga gttggtctaa
    47221 gctcacttga aaaatttgac ttaggagtaa gaggctatgg atgtctgggt aagttttgtc
    47281 attttcccag ttagatatgc tctgatggct tacatagact ttctcggcta actcttcttg
    47341 cgagagcgct tgttgtttac ggtagcgaat aatttgctga ccaatttcca taaatatctc
    47401 ctttcataat attagtataa gaatgataag ctaacgggtc tatcaaatgt atttgacatt
    47461 agggatagat cactatttat aaggtattaa aagacaatct gaggacaact ttacagcggc
    47521 gtaaaggaat tgtaaacaaa aagtgaaccg tcatagttca cttgtgatta agcatttttc
    47581 tgttttaagt aggtctctag cattttcttt tgtggtgttg caaagggata cagagaaaag
    47641 tcctcaattg ctacccactt taggtgagga gcattaggta aatctgttgc tttgaccacc
    47701 ccctctatga gttctattgt ccacttctgg tgactaaaag tgtgcttaat atttggaaag
    47761 tgattatcag tccattgggg ctttaattga tattctcttt cgaaagtttc attttgggtt
    47821 tgccagatta tggggttgga ttgattgtca tcaaataaat ctaattgttg agaaagaggt
    47881 gacgtttcga taataggaaa tgaccaaaaa cctcctagaa gtctcccttt agtatttttc
    47941 tccagtagat attggccact ttggttacga atgacaaaag cctgaattcg cattggttta
    48001 ggctttttct ttggattttt tatgggatat ttaccataag taccattcaa ataagcagcg
    48061 ttaaagaaac gaatagggct ttcatcaggc ctaggtgtct tagcggattc gatgtctgta
    48121 cctagatcca taagtgcctg gttaaaatca ccgggtctgt cagggtcaat tagtatttcc
    48181 ataatagctt gaaaaatttt acgattttta gggtcaccga tatcataatt aacttcaaac
    48241 aaacgagcca tgacacgcat cacattgcca tcaacagcag gctctggtaa gttgaatgag
    48301 atgctggcga tagcacctgc agtataagga ccaatccctt ttaatgaagc aatatcatca
    48361 taagtgtgag gaaatatgcc accaaaatca accatcactt gctgtgcagc cttttgcata
    48421 ttacgcaccc gagaataata gcctagtccc tcccatgcct ttagtaattg ttcttcagga
    48481 gcgtctgcca aatcttttat ttgaggaaac cactctagaa aatgtttata ataagggatt
    48541 accgtattaa cttgtgtttg ttgcaacatg atttcagaaa cccaaatata gtatggatta
    48601 gtagttcttc gccaagggag atcacgtttt tcttggtcat accaccctaa aagtgtgcga
    48661 cgaaaagaag cgattgtctc attatcccac atattaatac cataatcttt taattcaatc
    48721 atgttgttat tgtaacacag gagccttaaa aagagtaaaa aaagatttta ggtatttgcg
    48781 aaaaagaaaa ggaaatatta taataatatt aggtttacct catctaaatg ttgatttaga
    48841 ataactgaaa ttaacgatag aagcagacag gttttaattt ggaggggaca tgttccagaa
    48901 attaaagaat cttattgatc ggtattcgtg gttcgttttt ataatgatga ttcttgtgtt
    48961 atttcttaac tatatttatc catttgatcc ttctttgagc ggactacagt tggaatggct
    49021 gatcatgacg caagtttttc ctggattagg tatcgtatgt gccatggttg ctattatttt
    49081 taaaaagttt aacctttttg ttttggccct aattttatat tttgcacttt ggttaaaact
    49141 tttccttgct ttttcttttt tcacctattt tttaggagtc tgactaagtg ccaattttta
    49201 aaactcgtga aagaaaatgt ttgacaaaac ttagtcaagt agaaaaagtt ccttgctttt
    49261 aattgactat tagtgtacaa taagattctg tgagtatacc tcacttactc actccaaaag
    49321 tagtgagtca ttagaccaaa aggaggaaaa tatcaatggc taaatacgaa attctttata
    49381 tcattcgtcc aaacattgaa gaagaagcta aaaacgcttt ggtagcacgc tttgactcta
    49441 tcttgactga caacggtgca aatgttgttg aatcaaaaga ttgggaaaaa cgtcgtcttg
    49501 catacgaaat caacgatttc cgtgaaggac tttaccacat cgttaacctt gaagcgactg
    49561 acgcagcagc tcttaacgag tttgaccgtc tttcaaaaat caatggtgac attcttcgtc
    49621 acatgatcgt taaacttgac gcttaattcc aactaagagg gtagtttatg attaataatg
    49681 tagtactagt tggtcgtatg accaaggatg cagaacttcg ttacacacca agtcaagtag
    49741 ctgtggctac cttcacactt gctgttaacc gtacctttaa aagccaaaat ggtgaacgcg
    49801 aggcagattt cattaactgt gtgatctggc gtcaaccggc tgaaaattta gcgaactggg
    49861 ctaaaaaagg tgccttgatc ggagttacgg gtcgtattca gacacgtaac tacgaaaacc
    49921 aacaaggaca acgtgtctat gtaacagaag ttgttgcaga taatttccaa atgttggaaa
    49981 gtcgtgctac acgtgaaggt ggctcaactg gctcatttaa tggtggtttt aacaataaca
    50041 cttcatcatc aaacagttat tcagcgcctg cacaacaaac gcctaacttt ggaagagatg
    50101 atagcccatt tgggaactca aacccgatgg atatctcaga tgacgatctt ccattctagt
    50161 cagaatggat tgaacgaata cgaaattatt tgagttcggg ctaaaaattt agaacttaga
    50221 atttagatca actccctagg aaatcaagat ttcttgccag ttttctaaaa ttcagtcgtt
    50281 ttttaagagc ccttaacatc ataatttata aaggagaaaa tacatggctc aacaacgtcg
    50341 tggcggattc aaacgccgta aaaaagttga cttcatcgca gctaacaaaa ttgaatatgt
    50401 tgattacaaa gatactgagc ttcttagccg tttcgtttca gaacgtggaa aaattcttcc
    50461 tcgtcgtgta acaggaactt cagctaaaaa ccaacgtaaa gtaacaacag caatcaaacg
    50521 tgctcgcgtt atggctctta tgccttacgt aaacgaagac taataaacaa acagtccatt
    50581 tcggactgtt ttttagttga ctaggactag tctaggcgta taggtgagtt ttttctaaaa
    50641 gagagaatga tagtaaacga aaatccttgc gtagacagca aggatgaaat gattgttatt
    50701 tgttaacagg tgacatagtg ttttgaatgt tgtatttgcg ttgtaggtaa tagcgtaaag
    50761 caagggaaac accaccaata atgagcaagg caagaggagg caactgtggg ttaagagagg
    50821 ttggtaaaaa ggcagttgca gagtacaagg caatccaaat caacatggct aatgataagg
    50881 ctgcgatgat tttgaaccaa cttggacgaa ggctcttatc ttttccgaga tgacggtaaa
    50941 taaagtaata agtagcgtac atggatgctc ctccaccaaa accaagtgcc agtaaggaaa
    51001 tcaatcctgt aactgtagcg tttgtgttga agaaggtcat gatgccattt aaaagggcaa
    51061 caattccgat gaatagcaaa gaggtatcta gccacatcaa ccaaggattg gtgtttttgt
    51121 cagtttctgc tgctctttga ctagggtctt ggctaaaaga agctgcccaa gcagtcggag
    51181 tgcctaatag atttcgagcg gtgacacctt ttttctggtt ttctagaata gctggaatgg
    51241 cttcttcgag aatcagctta atgtcttcat cagacttgcc gtcttggatt aatttgttcg
    51301 tagcggtgtg gataaattct tgattttttt tggtaagttc ttgtaattcc atgggaatcc
    51361 cttctttata tgggttagtt aaagtaagtc aatggtatta gctagttgta atgaaacttt
    51421 ggagctagcc gtttgacaca gagttttgtc aggtcttaac cgcattctgc agcttgctcc
    51481 actctagaac catttttttc taataaagta aatcacaaca aaggaactaa gtagcatagc
    51541 aatcaaggta atataccaaa aagcatgttc taggccattt agtggtaacc agttgttttg
    51601 gaaattcata ccataggcgg agaaaatcac agttggtata tctaaagcca tggtcatcaa
    51661 ggccagtgtt ttcataatgg tgttctggtt attgttgata atggaagcag tggtttcggt
    51721 catggcattc aagacatttt cataaatgcc agccatctca atcgcctgtt gggtttcaat
    51781 caaggtatct tctagcaaat cctcgtcctc aatgtatttc ttgagagagc ttgttgagct
    51841 ggataatttc ttgacgatgc gttcgttgaa tttcaaggaa gcctttaagt agacaatgga
    51901 tttttcaagt tccatcatgt cgataagttc ttcatttcga gtagctgctt caagctgagc
    51961 ttccaaacgt tcgctttggc gatcaatggt cctcaaggca gtcagaaaga gctcggcgtt
    52021 gcgatataga atctgaaaaa caaaacgtgt cttcatgaag gtatagaaat ttttgacccg
    52081 gcggttgtga aaatgatcga aaagggtcat gtcatgcaga caagttgtga taacagcatt
    52141 ttccgttaca ataatgccta aaggcatagt aatatagtaa cttttattgt tacgttcttc
    52201 gtatatcgga acgtctacga taattagggt ataatcatct tctacggcaa tacgtgaggt
    52261 ttcttccacg tccaaaggcg cgcgcaagtc agaaatatca atgttaaatt gatcggcgat
    52321 cttcatagac tcttcttggg aagggttaac cagtttaatc caagctcctg gctcgaatgt
    52381 ttctatttct ttgaattcaa ttgccgatga aagaaacatt tgtttcatga caagccctcc
    52441 tttttgatga ttgactggta gagattgaca tcaaaccttg ataataaagc cttctcaagg
    52501 attttctgaa aaattctcaa tacttatcta ttataccaaa attcgcaaag tttggggcaa
    52561 taggttatca gaaatttttg ttataatata agaaagctcg atgaggaggg tgcctttagg
    52621 cacattcaag atgcaaaata aaataataat tcatggggca agagcccaca atttaaaaaa
    52681 tattgacgtg gaaattccac gagacaaatt agttgtcgtg acagggttat cagggtcagg
    52741 aaaatctagt ctagcctttg atactattta tgcggaaggg caacgtcgtt acgtagaaag
    52801 tttatcagct tatgctagac agttcttggg taatatggaa aaaccagatg tggattccat
    52861 tgacggtctt agccctgcta tttccattga tcaaaaaact accagtaaga acccccgctc
    52921 aacggttggt acggttacgg aaatcaatga ttatttacgt ttactatatg cgcgtgtggg
    52981 cacaccttac tgtattaatg ggcatggggc cattacagca tcttctgcag aacaaattgt
    53041 agagcaggtc ttagccctcc ctgaacggac acgcatgcag attttagcgc ctattgttcg
    53101 ccggaaaaaa ggtcaacaca aaaccatttt tgagaaaatt caaaaagatg gttatgtccg
    53161 tgtgcgggtt gatggggata tttttgacgt gacagaagtg ccggagctgt caaaaagtaa
    53221 gatgcacaac atcgaggtgg ttattgatcg tctggttaat aaggatggta ttcgtagccg
    53281 tttatttgat tcggttgaag cagctttaag gctgggagat ggctacctta tgattgacac
    53341 tatggatggc aatgaactgc tcttttctga gcattattcc tgtcctgtct gtgggtttac
    53401 ggttcctgaa ttagaaccaa gactcttctc ttttaatgcg ccgttcggct cttgtcccac
    53461 ctgtgatggg ctaggcatta agttggaagt ggatttggac ttggttgttc ctgacccaag
    53521 taagtctttg aaagagggag ctttggcgcc ttggaatcct atttcctcca attattaccc
    53581 aaccatgctt gagcaagcta tggcaagttt tggggtggat atggatacac cttttgaggc
    53641 cttgactgag gaagaaaggg atttagtgct ttatggttca ggtgatagag aatttcattt
    53701 tcactatgtg aatgattttg gtggtgagcg taacattgac attccttttg aaggtgttgt
    53761 gactaatgtt aatcgccgtt atcacgaaac taatagtgat tatacgcgca atgtgatgcg
    53821 tggctacatg aatgaattga cttgtgcgac ttgtcatggt taccgtttga atgaccaagc
    53881 cttgtgtgtg catgtgggtg gcgaagaagg acctcatatt ggtcaaattt cagaattatc
    53941 gattgccgac cacttgcagt tgttggaaga actggaactc acagaaaatg aaagtaccat
    54001 tgctaaaccg attgtcaaag aaattcatga ccgcctgacc ttcttaaata atgtgggact
    54061 gaattatctg accttatcac gtgctgctgg gactttatca ggtggggaaa gtcaacgaat
    54121 caggttagcg acacaaattg gttctaactt atcaggggtg ctctatatct tggatgagcc
    54181 ttctattgga ttgcaccaaa gagacaatga ccgtttgatc gaaagcctga aaaaaatgcg
    54241 tgatttgggt aataccttaa ttgtggtaga acacgatgaa gacaccatga tgcaggcaga
    54301 ctggttgatt gatgtggggc ctggggcagg tgagttcggt ggagaaatta ttgcctctgg
    54361 aacgccgaaa caagtggcta agaataaaaa atccatcacg ggtcaatatt tgtctggtaa
    54421 aaaattcatt ccagtgccat tagagcgtcg ttctggtaat ggccgattca ttgaaataaa
    54481 aggagcagcg cagaataatc ttcaaagcct cgacgtgaga ttcccactgg gtaaatttat
    54541 tgcggtcaca ggggtatcag gctcagggaa atcaaccttg gttaatagta ttttgaaaaa
    54601 ggctgtcgcc caaaaattaa accgtaatgc tgataagcct ggtaaatatc actctatttc
    54661 tggtattgaa catattgaac gcttgattga cattgaccaa agtccgattg gaaggacgcc
    54721 acggtcaaat ccagccacct atacgggtgt ctttgatgat attcgagact tgtttgccca
    54781 aaccaacgaa gctaagattc gtggttacaa aaaaggccgt ttctctttta atgtcaaagg
    54841 tgggcgttgt gaggcttgtt caggtgatgg gattatcaaa atcgaaatgc actttttgcc
    54901 agatgtttat gtgccttgtg aggtttgcca cggcagacgg tataattctg agacgcttga
    54961 ggtgcattat aaagaaaaaa atattgctga agtgcttgac atgactgtgg atgatgcctt
    55021 ggtatttttc tctgctattc ctaaaatcgc tcgaaaaatt caaacgatta aggatgttgg
    55081 acttggttat gtgaccttag gtcaaccagc tactactcta tctggaggag aagcgcagcg
    55141 gatgaaatta gctagtgaat tgcacaaacg ctcgacaggt aagagcttgt atatcctaga
    55201 tgagccaacg actggtttac acacagatga tattgcaagg ctattaaaag ttttggaacg
    55261 ctttgtagac gatggtaata cggttcttgt gattgagcat aatttggacg ttatcaaatc
    55321 tgctgaccat attattgact taggaccaga aggtggtgtc ggaggtggtc agattgttgc
    55381 cacggggaca ccagaagagg tagctcaagt taaagaaagc tatactggtc attatttgaa
    55441 agtaaaacta caacaatagg attaggagtt cgtttgagct ccttttaatc ttttaaatgc
    55501 tctcaaatca tggtaaactt aaagtagttt tttcatgcta caatattctc aaaagttacc
    55561 aaaggagttc gcgatgtcag gatttttaga gcaacgatta ggtcactgcc taaggcagat
    55621 ggcagagaag gggctagagg ctcttctggt cacccattta accaatagtt attacttgac
    55681 aggtttttct ggaactgcag caactgtttt gataacggcc aaacgtcgtg ttttgatcac
    55741 agattcacgt tataccttgc ttgctaaagc tagtgttgag ggatttgata ttatcgaaag
    55801 ccgcacgccc ctgaatgttg tggcagaatt gttaggggct gatcaaatag attgccttgg
    55861 ttttgaggac caggtatcgt tttcttttta tcaggccatg caagcagaac tgtcaggaat
    55921 aaccttgctt gctcagtcag gctttgtgga gcatttacgt cttattaagg acgtctctga
    55981 aattgagacc attgctaaag cgtgctcgat ctcagacaaa gcatttgaag atgctcttga
    56041 ttttattaaa ccagggacaa ccactgaacg tgacctggct aattttttag attttcgcat
    56101 gcgtcagtat ggtgccagcg gcacatcatt tgatatcatt gcagcttcag gctatcgctc
    56161 tgccatgcct catggacgcg ccagtgacaa ggttatccag aataaagaga gcttgaccat
    56221 ggactttggg tgttactaca atcactatgt tagtgatatg acgaggacca ttcacattgg
    56281 ccaagttact gatgaagaac gtgagattta tgctcttgtt cttgctgcta ataaggctct
    56341 aattgctaaa gctagcgctg gcatgactta tagtgacttt gacggtattc cgcgccaact
    56401 tatcactgag gcgggttatg gcagtcactt cacacatggc attggtcatg gtatcgggct
    56461 tgacatccat gagaatccat ttttggggaa atctgagcaa cttctccaag ctggaatggt
    56521 ggtcacagac gagccaggta tctatttgga taacaaatat ggtgtccgta ttgaagatga
    56581 cttggttatc acaaaaactg gttgtcaagt cttgaccttg gcacccaaag aattaattgt
    56641 attgtaaaag aatggttcag gaaaggatta ggggagaagc aatgacaaat cgtttatcat
    56701 ggcaagacta ttttatggcc aatgcagagc tgatttccaa gcgctctacc tgtgatcgcg
    56761 cctttgtggg agctgttttg gtaaaggata accgcattat tgccacaggt tacaatggcg
    56821 gagtttctgc tacggataac tgcaacgaag cgggtcatta tatggaagac ggtcattgta
    56881 ttcgcactgt tcatgctgaa atgaatgctt tgattcaatg tgcgaaagaa ggaatttcaa
    56941 cggacggaac tgaaatttac gtgactcatt ttccttgtat taactgcact aaggcgcttt
    57001 tgcaggcggg gattacaaag attacctata aagcacatta tcgtccgcac ccctttgcca
    57061 ttgaactgat ggagaaaaaa ggggttgcct atgtgcaaca tgatgtgcct caaattgttt
    57121 taggaggcga taaggaacaa tagaaagtgt acctaaaagg acggtcataa aagatttgag
    57181 ccaaggccac ttttgtggta aaatgaagtg ataaatattt tagtaagagg taataataat
    57241 gattgaagca agtaagctta aagcaggtat gacatttgaa gcagaaggaa aattaatccg
    57301 tgtccttgaa gctagccacc acaaaccagg taaaggaaac actatcatgc gtatgaaact
    57361 acgtgatgtg cgtacaggtt ctacttttga cacaacttac cgcccagatg aaaaatttga
    57421 gcaagccatc attgaaactg tcccagcaca atacctatac aaaatggatg acactgctta
    57481 cttcatgaac actgatactt atgatcagta cgaaattcca gttgctaacg ttgagcaaga
    57541 attgctttac attcttgaaa actcagacgt gaaaatccaa ttttatggaa gtgaagtgat
    57601 tggggtaacg gttccaacaa ctgttgaatt gaccgttgcg gaaacacaac catctattaa
    57661 aggagcgaca gtgacgggtt cagggaaacc tgcaactctt gagacaggac ttgttgttaa
    57721 cgttccagac tttatcgaag ctggccaaaa actaatcatt aacactgcag aaggtactta
    57781 cgtttctcgt gcttaatcct ttgtcaaaac agattagtct aaaaaagaga aaggaaatgc
    57841 tatgacaact gaatatatcg gtgaaatcgt catttcacca cgagtgcttg aggttattac
    57901 ggggattgct acgacacaag ttgagggcgt ccactcactc cataacaaga aaatggcaga
    57961 tagctttaac aaagcaagtc ttggtaaagg ggtttatctc cagacagaag aagatggaag
    58021 tgttacagca gatatttatg tttacttgca atatggagtt aaggtcccga ctgtatctat
    58081 gaatattcaa aaaacagtca aatctgctgt gtacgatatg gcagaagtgc ctatttctgc
    58141 agttaatatc catgttgaag gtattgttgc tgaaaagaca ccaaaaccag atttgaagtc
    58201 actttttgat gaggatttct tggatgacta acagctttca aaattcaaga agagatttgc
    58261 gtgaacgtgc ctttcaggct ttatttaaca tagagatggg agctgagtta ttggcagctt
    58321 ctcagtttgc ctatggttat gataaagtaa caggggagga cgctcaagta ttggagctgc
    58381 cgattttttt attaagtctt gtgacgggtg tcaataatca taaagaagag ttggataact
    58441 tgatatcaac tcatttgaaa aaaggttggt cgttagaacg cttgacacta acagacaaaa
    58501 cgttattacg actaggtctt tttgaaatca agtattttga cgaaacacca gaccgtgttg
    58561 cactaaacga aatcattgaa gtagctaaaa aatattctga tgaaacctct gctaaattta
    58621 ttaatggctt acttagccaa tatgtctcgg aagctccttc agcaaataaa tcataataag
    58681 tcggagcaat cttaggattg tatccgattt tttcaattta tgtacgaata ttaaaagaca
    58741 aacaagcata ctctcgcatc tagaaagagt atgcttgttt gtcttttaga gtaattagtg
    58801 attggttatg ttttgtttaa atacttgctc ctggaagtag cgtaatcggt aaaatatagt
    58861 ctttactggt tttttctttt ttaatttttt tgatgagtat ttcgacactc aatttagcaa
    58921 tctcatcaat aggttgacga atggtcgcta attgaggaac atattgctgg ataaatgtcg
    58981 ttccatcata acctatgact ttcatatcct ctggaatagt aatgtgaagt tgtttggcta
    59041 ctttcataat aaggatagca gttaaatcat cagaaacgaa aaggccatca ggttttcggg
    59101 ttgccaaaat ggatttaatt tccatttcgc gtctaacagg agataggtta tttggtaatt
    59161 tgaggatttc tgcacttcgt ttgagctggt agttaaaccc tagctggcgc aaccctgtgg
    59221 gcgaatcaga attgtcatta cctgtaatca tgacaatgtt ttgacaaccg tgtttttgca
    59281 aagtttgtgc ggctaattta ccaccttcaa aattatctga agaaataacc gggatatttg
    59341 gcgctagatt acggtcaaaa gctacaatag gagcttcgac acgctcataa tcctcaattc
    59401 ccaaattatg actggaagaa ataatgccgt ccacttgatt ggccgcaagc atttctaagt
    59461 attcgcgttc tttgacggga ttatgttcac tattgcaaat aatagttttg tagccctgtt
    59521 taaacaattc aatttccaaa tgttcaatta attcagcgta aaagatattg ctaatgttgg
    59581 ggaagataag cccaatgagt tgggttgatt taccttgcaa acttctagcg agattgttag
    59641 gcttgtagcc caattcacgc atggccttat tcaccttatt cactgttttt tgggaaaggt
    59701 agcctttttt gttgattact cgcgaaacag ttgttggact gacacccgcc aaggctgcca
    59761 cgtctgttaa ttttgcgacc acttgcctaa tacctcattt cataataatg tccaaaagct
    59821 tgtccttcta ctacttggat acctgtttgt ccttgggttg ggaaaacacg actcgttagg
    59881 actttttgcc cttggttaat aaagatttca agaatggaat tgtccacata gacattaagt
    59941 gtaacatggc cttgtgggat ttggcaagag cgcatcgtac catagtcttg ggcatattga
    60001 acccctgccc gacttcggtc aatgcttaac tgtccttttg ttgtatccac cttcaatctt
    60061 aggccattcc ctttttggtc ggcaaatagg agcaggtcaa gctttttttg gcctggaatc
    60121 gtcaattcca actcataaca gttagaactg tctattttat tatgaaataa ttctgctggc
    60181 cctcgaagat tttgtagggc tgaaacaggt gtttgataga gaattccgtc tttaatcctt
    60241 aattctttca ctaaactaag agctccttga tagtcataac gatctgtcgg ataatcaata
    60301 tctggtaacc caatccaaga cacggctaga accctgccat ctggactact aaaggcttga
    60361 gtggcataag cttcaaaacc aaagtcaaga ttttgaaggg cccttccacc cagcaattgg
    60421 ccagtttctg gattgaagct ttcaaaaatt ttataggtat tggggtagat attttgataa
    60481 tctaaatcag ctttagccag tccttgtggg ctgaaaatca aaacaggctt gtcatcgaca
    60541 aacacaaggt tggggcattc aatcatgtat tccgtaccag aatcgtcaag gtccaaatcg
    60601 gctataaatc gccagttgtc cacatgattg tccacagctt tgtaaagttt gatttttcct
    60661 ttgccatcta aaccttgggc gccaataata gcatagaatt gaccttgata agaaaaaagc
    60721 tgaggatccc gaaaatgttc tgtaacatca tcgggctgtt caatcaaaac ctgtggtatt
    60781 ttggaaatat ttccttgttt atccatccat gcgccaacct gtaatggtgt tctgacccaa
    60841 ttggcatcgc gaacattacc agtgtaaaag aggaagagct tgtcatcaat agcatatgca
    60901 gaacctgagt aagccccatg actatcgtga gcgtggtctg gcagaaggcg gctccttgtt
    60961 tccgtaaaat ggacgaggtc tgtggatgtc atgtgtaccc attgtttcag accatgcgca
    61021 gcgccataag gccagttttg ataaaaaaga tggtagcgac cgttgaaata agaaaaccca
    61081 ttggggtcat tgagtaatcc tgtcttaggt tcgacatgaa attggctgtg ccatggggat
    61141 tgggccattt tttcagtaat agcttggtag tcttttgagc tccactcttt gtaagggcgg
    61201 tagcgaatag cttgtggtag atccatcaaa atctccttgt tttatagtcg tcttcaagaa
    61261 tatactaaac gttttctgtt taaaaatcaa gaatttaaag catataaaat aaaaatatgt
    61321 taaacgcttg acataaaaaa tattagtgat aaaatgatag tcaagaagtg aagcggattc
    61381 actagcgttg gtcatgcaca ttcttagcca caaagctggc agaatggagg agggactaac
    61441 accaaatgaa aaaggagact tttaggcatg gataatcgtc agattgcagc tgaagtgatt
    61501 gaggctttag gtggtcgaga aaatgtgaga agtgttgccc actgtgcaac tcgccttcgc
    61561 gtgatggttt atgatgaagg aaagattgat aaggaaaaag cagaagctat tgacaaggtt
    61621 aaaggagctt tctttaattc tggtcaatat caaatgattt ttggaactgg taccgttaat
    61681 aacatttatg acgaagtcgt tgctcttggt ttaccaacat catcaaccag tgagcaaaag
    61741 gcagaagcag gcaaacaagg caatatcttc caacgggcga ttcgtacgtt tggagatgtc
    61801 tttgttccca ttattccagc tattgtagca acgggtctct ttatgggggt tcgtggtttg
    61861 gtgacccagc cagctattat ggatttattt ggggtgcatg agtacgggga aaattttctc
    61921 atgtacactc gtattctaac ggatacagcc tttgtctact tgccagcttt ggtggcttgg
    61981 tcagccttta gggtctttgg gggtaatcct attatcggta ttgttttagg attgatgctg
    62041 gtttccaatg agttgcctaa tgcttgggta gttgcctctg gtggagatgt taagccgcta
    62101 accttctttg gatttgttcc tgttgttggt tatcaaggta ccgttttacc cgccttcttt
    62161 gttggtctgg taggagctaa gttggagaaa tggttgcaca aaaaggttcc agaagcttta
    62221 gacttattgg tcacaccgtt tttaacgttt gctattatga gtatcttggg actatttgtg
    62281 attggaccag tcttccattc tcttgaaaac cttgttctgg ctgggacaca ggccgtcttg
    62341 catttgccgt ttggcattgc tggtcttatt gttggtggta tccaacagtt gatcgtcgtt
    62401 actggtattc accatatctt taacttccta gaagcgcagc taattgccaa taccggaaaa
    62461 gatcctttca atgcttacct aacagcagca acagctgctc aagctggagc taccttagct
    62521 gttgctgtta aaaccaaatc aacaaaacta aaaggtctgg cctttccatc aaccttgtca
    62581 gctcttttag ggattactga accagctatt tttggggtca acctccgtta tccaaaagtc
    62641 tttgtttcag gtcttattgg tggtgcctta ggtggttggg tcgctgggct ctttggcatt
    62701 gcaggaactg gttttgggat tacagtctta ccaggaacgc tcctttactt gaatggccaa
    62761 ttattacaat accttgtgac tatgcttgtc ggtcttgggg tagcctttgc aattgcttat
    62821 acttggggtt atcaagatag agaaaccctt ccgttaccag ctgtcgaagt tgatcaaacg
    62881 gctgatcagc cagccttagc agaggaaact ttatatagtc ctttgaatgg aacagttgta
    62941 gatttagcag ctgtttcaga ccctgttttt tcatcaggtg ctatgggtca aggcttagca
    63001 attaagccag aagataatac cctatactca ccagttgatg gtaaagttga aattgtcttt
    63061 gaaacaggtc atgcctatgc tataacgtca agtcaaggag cagaagtgtt acttcatatt
    63121 ggtattgata ccgtgtcgat ggcaggagat ggctttgaat ctcttgtagc agtaggtcag
    63181 gcggttaaaa aaggtgatct tcttggacac tttgatccta gcaagattgc agaagctgga
    63241 ctagatgaca ccaccatgat gattgtgacc aatagcactg actatcaaag cgtggacatt
    63301 cttgctcaag gacatgtttt gattggtgat caagtggcac ttatcaaata aggacaaaag
    63361 agctaactaa gcagctcttt tgtcctgcca tgaatggcag gttggcaaaa aaatgagaaa
    63421 agcctaaaaa ccttaaattt gtgttatttt acctatactt tgtaccttgt tttttttaat
    63481 aaagtgttgt tatacttaag gcgaactata ggaatgcgct tacatagatg gtatatcaac
    63541 tgggaagcca tgacttagta ccaaaaataa ggagtgtcca aatggataaa catttgttgg
    63601 taaaaagaac actagggtgt gtttgtgctg caacgttgat gggagctgcc ttagcgaccc
    63661 accatgattc actcaatact gtaaaagcgg aggagaagac tgttcaggtt cagaaagaat
    63721 tatcttctat cgatagcttg cattatctgt cagagaatag caaaaaagaa tttaaagagg
    63781 agctttcaaa agaaaaagta ccagaaaagg tcaaagagat attagcaaaa gctcagcagg
    63841 cagataaaca agctcaagaa cttaccaaaa tgaaaattcc tgagaaaata ccgatgaaac
    63901 cgttacatgg tcctctctac ggtggttact ttagaacttg gcatgacaaa acatcagatc
    63961 caacagaaaa agacaaagtt aactcgatgg gagagcttcc taaagaagta gatctagcct
    64021 ttattttcca cgattggaca aaagattata gccttttttg gaaagaattg gccaccaaac
    64081 atgtgccaaa gttaaacaag caagggacac gtgtcattcg taccattcca tggcgtttcc
    64141 tagctggggg tgataacagt ggtattgcag aagataccag taaataccca aatacaccag
    64201 agggaaataa agctttagcc aaagctattg ttgatgaata tgtttataaa tacaaccttg
    64261 atggcttaga tgtggatgtt gaacatgata gtattccaaa agtgaatggg aaagctagtg
    64321 acgaaaacct caagcgatct attgatgttt ttgaagaaat tgggaaatta attggaccaa
    64381 aaggtgttga taaatcgcgt ctgtttatta tggatagcac ttacatggct gataaaaacc
    64441 cattgattga gcgaggagct ccttatattg atttgttatt ggtacaagtc tatggcgcaa
    64501 gaggagaaca aggtgagttt caaaacgata ccaagttagt taccgagact cctgaagaac
    64561 gatggcaagg ttatagcaag tatattcgtc ctgaacaata catgattggt ttttctttct
    64621 atgaggaaag agcaggcagc ggcaatcttt ggtatgatat taattctcga aaggacgatg
    64681 acaaagcaaa tggaattaac actgacataa ctggaacgcg tgccgaacgg tatgcaaggt
    64741 ggcaacctaa gacaggtggg gttaagggag gtatcttctc ctacgctatt gaccgtgatg
    64801 gtgtagctca tcaacccaaa caaatagctg aaaaagataa acagaatgta aaaaataatc
    64861 agcctcagat tccagagatt actgataaca ttttccactc agattatagt gtctctaaag
    64921 cattaaagac agttatgcta aaagataagt cgtatgatct gattgatgag aaagatttcc
    64981 cagataaggc tttgcgagaa gctgtgatgg cgcaggttgg aaccagaaaa ggtgatttgg
    65041 aacgtttcaa tggcacattg cgattggata atccagcgat tcaaagttta gaaggtttaa
    65101 ataaatttaa aaaattagct caattagact tgattggctt atctcgcatt acaaagcttg
    65161 accaatctgt tttacccgct aatatgaagc caggcaaaga taccttggaa acagttcttg
    65221 aaacctataa aaaagataac aaagaagaac ctgctactat cccaccagta tctttgaagg
    65281 tttctggttt aactggtctg aaagaattag atttgtcagg ttttgaccgt gaaaccttgg
    65341 ctggtcttga tgccgctact ctaacgtctt tagaaaaagt tgatatttct ggcaacaaac
    65401 ttgatttggc tccaggaaca gaaaatcgac aaatttttga tactatgcta tcaactatca
    65461 gcaatcatgt tggaagcaat gaacaaacag tgaaatttga caagcaaaaa ccaactgggc
    65521 attacccaga tacctatggg aaaactagtc tgcgcttacc agtggcagag ggaaacattg
    65581 atttgcaaag ccagcttttg tttgggactg tgacgaatca aggaacccta atcaatagcg
    65641 aagcagacta taaggcttac caaaatcata aaattgctgg acgtagcttt gttgattcaa
    65701 actatcatta caataacttt aaagtttctt atgagaacta taccgttaaa gtaactgatt
    65761 ccacattggg aaccactact gacaaaacgc tagcaactga taaagaagag acctataagg
    65821 ttgacttctt tagcccagca gataagacaa aagctgttca tactgctaaa gtgattgttg
    65881 gtgacgaaaa aaccatgatg gttaatttgg cagaaggcgc aacagttatt ggaggaagtg
    65941 ctgatagtca agcagcttta aaagcctttg atggggtaat agagtacaat ccgatatcta
    66001 ctcagaatag agcttcgatt atctttgaaa tgaaagaccc tagtttagca aaatattggc
    66061 gccttttcaa tgatagcagt aaaggcgaag acgactatat caaagaagca aaacttgaag
    66121 tctttacagg ccagctgaat gctgaagcag atgtgaaaac gagcttggaa aaatcagatg
    66181 attggcaaac tgtttcaacc tattcaggac aagaacaggt atttagccat gcactggata
    66241 atattagtgc taagtactgg agagtaaccg tagatactaa gggaggaaat tatagttggc
    66301 catcactccc tgaactccaa attttaggtt atccgttacc taacgccgac actatcatga
    66361 aaacagtaac tactgctaaa gagttgtctc aacaaaaaga taagttttct caaaagatac
    66421 ttgatgagtt aaaaataaaa gagatggctt tagaaacttc tttgaacagt aagatttttg
    66481 atgtaactgc tattgatgct aatgctggag ttttgaaaga ttgtattgag aaaaggcagc
    66541 tgctaaaaaa ataaacaaag taactctctt agatagcaac attcagatta aattaacaaa
    66601 atgtgactat gataaaggtt tgctggaatt gattaaccaa aagactaaaa atctgagatg
    66661 aatagtccca gatttttagt cttttatagg ttttgatgac ataaagctaa ataatcgtta
    66721 gactaccaga aagtggcgct tgtccgtgag acatggctgt cttgataaga gcctgtgtca
    66781 tcataaagat aaaaaagaac aagaaaaagg agaaaacatg ggaaaattat atggaagtat
    66841 tgaagctggt gggacaaagt ttgtctgtgc cgtgggtgat gaggagttta cagtggtgga
    66901 taaaacacaa tttccgacca ccacaccaga agaaaccatc gcgcaaacca tcgcatattt
    66961 taaaaccttt gaggccgatt tggctggaat ggcgattggt tcttttggac caattgatat
    67021 tgatccgtcg tcagagactt acggttatat cacaaccact cctaagtcag gctgggctaa
    67081 tgtggatttg ttaggacaac tttcggctgc ttttaagatt ccttttgatg tgacaacaga
    67141 tgttaatagc tctgcttatg gtgaggtgct tgctcgtcct ggtgtggaaa gtttggtcta
    67201 ttacaccata ggtactggta ttggggcggg agctattcaa catggccact ttattggggg
    67261 attaggtcat acagaagcgg gtcataccta tgtcatgctc catcctgatg atatggccaa
    67321 gggattttta ggtgtttgcc cttttcataa agggtgtctg gaagggatgg cagcaggtcc
    67381 aagtattgag gccagaacgg gagtgagagg tgaacggctt gatcaggaag ctgatgtttg
    67441 ggacattcaa gctttctaca ttgcccaagc agcgctgcaa gcaactatgc tttaccgtcc
    67501 tcaagtgatt gtttttggcg gaggtgtcat ggcgcaagaa catatggtgt tgcgcgtgca
    67561 tgataaattc actacccttt taagcggtta tttgccagtc cctgatttga cagactacat
    67621 tgttactcca gctgttgctg ataatggctc cgctactctg ggaaattttg ctttggccaa
    67681 gttggctgca caaggaaaat agggtctgtt tttgcctgct tgtctagtaa aagaaagcgc
    67741 agtcagctag aacatgctat aataaacaga gattaacatc agaagcgagg aatattatgt
    67801 cagaaccatt atttctaaaa tcaaccatgc acgacagaat ttggggtggc accaagttga
    67861 gagatgtctt tgcttacaac atcccaagtg acaccacagg tgagtattgg gctatttctg
    67921 ctcatcctaa tggtgtttcg acagttacta atggacgtta tcaaggacaa cctttaaata
    67981 cattatatgc gcaagagcca gccttgtttg ggaatccaaa ggaagaagtt tttccactgt
    68041 taactaagat tttagatgct aatgactggc taagtgtcca agttcatcct gatgatgctt
    68101 atggacgtga acatgaagga gaattaggca agacagaatg ctggtatatt atttctgccg
    68161 aagagggttc tgaaattgta tacggccatc aggccaagtc caaagaagac cttcgtgcca
    68221 tgattgaagc aggtgcttgg gatgatttgc tgactcgtgt cccagtcaaa gcaggcgatt
    68281 ttttctatgt tccaagtggc accatgcatg cgatcggcaa gggaattctt attttagaaa
    68341 cccagcagtc atctgatacc acttatcggg tttatgattt tgaccgtaag gacgttaatg
    68401 gtaatcttcg tgaccttcat attgaaaagt ctattgatgt cttaacgatt ggtaagccag
    68461 aaaatagcgt gccagccact atggttttgg ataatatggt agcaacaact ttagtctcaa
    68521 cgccattttt cactgtttac aaatgggtga ctagtcaaat ggttgacatg aaacaagctg
    68581 cgccttatct tttagtgagt gttttaaaag gtcaaggaaa actctatgtg gaccagaagg
    68641 cttatgagct agaaaaaggc atgcacttta tcttgcctaa cgatgttaaa tcgtggtcat
    68701 ttgatggtca attagaaatg attgtgagtc accccaatca actcgtttaa taccatggta
    68761 tcaatatgag aaaaaaggat aaaattatga ctctctgttt ctctgataac ttt
//
LOCUS       DATURV010000004        63300 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DATURV010000004 DATURV010000000
VERSION     DATURV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63300
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.004"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(28..786)
                     /locus_tag="VCF75_000221"
     CDS             complement(28..786)
                     /locus_tag="VCF75_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2545819.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(783..1652)
                     /locus_tag="VCF75_000222"
     CDS             complement(783..1652)
                     /locus_tag="VCF75_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2545820.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERFFKGVRLTR"
     gene            complement(1997..2995)
                     /locus_tag="VCF75_000223"
     CDS             complement(1997..2995)
                     /locus_tag="VCF75_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP2545821.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANRQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAIKREDFPSASFIEN"
     gene            3349..5001
                     /gene="fbp54"
                     /locus_tag="VCF75_000224"
     CDS             3349..5001
                     /gene="fbp54"
                     /locus_tag="VCF75_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEP2545822.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSEMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            complement(5060..6307)
                     /locus_tag="VCF75_000225"
     CDS             complement(5060..6307)
                     /locus_tag="VCF75_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEP2545823.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDLEGLTEVAREKLLQAVAISSEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLMYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(6297..7478)
                     /locus_tag="VCF75_000226"
     CDS             complement(6297..7478)
                     /locus_tag="VCF75_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP2545824.1"
                     /translation="MPLIPDKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINRLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(7536..8012)
                     /locus_tag="VCF75_000227"
     CDS             complement(7536..8012)
                     /locus_tag="VCF75_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEP2545825.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            complement(8233..9273)
                     /gene="rfbB"
                     /locus_tag="VCF75_000228"
     CDS             complement(8233..9273)
                     /gene="rfbB"
                     /locus_tag="VCF75_000228"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEP2545826.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(9517..10110)
                     /locus_tag="VCF75_000229"
     CDS             complement(9517..10110)
                     /locus_tag="VCF75_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEP2545827.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYALVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(10110..10979)
                     /gene="rfbA"
                     /locus_tag="VCF75_000230"
     CDS             complement(10110..10979)
                     /gene="rfbA"
                     /locus_tag="VCF75_000230"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEP2545828.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHKLAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(11037..12143)
                     /locus_tag="VCF75_000231"
     CDS             complement(11037..12143)
                     /locus_tag="VCF75_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEP2545829.1"
                     /translation="MKIAIVGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGNDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KASGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLRPLGYQVDVRPQKGQ
                     LLDYDVHHIISDTYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKTCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLWSAGKA"
     gene            complement(12183..12971)
                     /locus_tag="VCF75_000232"
     CDS             complement(12183..12971)
                     /locus_tag="VCF75_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEP2545830.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMEGDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISIYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(12961..13647)
                     /locus_tag="VCF75_000233"
     CDS             complement(12961..13647)
                     /locus_tag="VCF75_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEP2545831.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(13719..14375)
                     /gene="nth"
                     /locus_tag="VCF75_000234"
     CDS             complement(13719..14375)
                     /gene="nth"
                     /locus_tag="VCF75_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEP2545832.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            complement(14372..15055)
                     /locus_tag="VCF75_000235"
     CDS             complement(14372..15055)
                     /locus_tag="VCF75_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEP2545833.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFHLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(15136..15654)
                     /locus_tag="VCF75_000236"
     CDS             complement(15136..15654)
                     /locus_tag="VCF75_000236"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEP2545834.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(15805..18015)
                     /gene="recJ"
                     /locus_tag="VCF75_000237"
     CDS             complement(15805..18015)
                     /gene="recJ"
                     /locus_tag="VCF75_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEP2545835.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKAWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(18012..18776)
                     /locus_tag="VCF75_000238"
     CDS             complement(18012..18776)
                     /locus_tag="VCF75_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP2545836.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKIVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAKEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFNQADPSGHYLESVGKFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(18776..19705)
                     /locus_tag="VCF75_000239"
     CDS             complement(18776..19705)
                     /locus_tag="VCF75_000239"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEP2545837.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(19720..20355)
                     /locus_tag="VCF75_000240"
     CDS             complement(19720..20355)
                     /locus_tag="VCF75_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEP2545838.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(20348..21586)
                     /gene="hflX"
                     /locus_tag="VCF75_000241"
     CDS             complement(20348..21586)
                     /gene="hflX"
                     /locus_tag="VCF75_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEP2545839.1"
                     /translation="MVETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(21659..22558)
                     /gene="miaA"
                     /locus_tag="VCF75_000242"
     CDS             complement(21659..22558)
                     /gene="miaA"
                     /locus_tag="VCF75_000242"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEP2545840.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLIDIREVTESYSAYEFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGTVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDHMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            22692..22868
                     /locus_tag="VCF75_000243"
     CDS             22692..22868
                     /locus_tag="VCF75_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEP2545841.1"
                     /translation="MVKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(22986..23894)
                     /locus_tag="VCF75_000244"
     CDS             complement(22986..23894)
                     /locus_tag="VCF75_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEP2545842.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFNYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FWGNLVLIAYYQYRFVFSFSWDNVSPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(24131..24550)
                     /locus_tag="VCF75_000245"
     CDS             complement(24131..24550)
                     /locus_tag="VCF75_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEP2545843.1"
                     /translation="MPFIAKQTLKSQLIPQDNLLADSRFNEIMDYLTGDFPFVFRPMF
                     NPHRYTISQDNQALEKVKQASYKRMGIAMTHLDGLIGESGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKIVEDSAVQQVLNAAH"
     gene            complement(24861..25847)
                     /locus_tag="VCF75_000246"
     CDS             complement(24861..25847)
                     /locus_tag="VCF75_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEP2545844.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGV"
     gene            26043..26345
                     /locus_tag="VCF75_000247"
     CDS             26043..26345
                     /locus_tag="VCF75_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545845.1"
                     /translation="MTYELCLEYGTYPLSRVDAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKCKYKDYPIKIETFLL"
     gene            complement(26708..26779)
                     /locus_tag="VCF75_000248"
     tRNA            complement(26708..26779)
                     /locus_tag="VCF75_000248"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26744..26746),aa:Arg,seq:ccg)
     gene            complement(26892..28097)
                     /gene="rpsA"
                     /locus_tag="VCF75_000249"
     CDS             complement(26892..28097)
                     /gene="rpsA"
                     /locus_tag="VCF75_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEP2545846.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            complement(28224..28295)
                     /locus_tag="VCF75_000250"
     tRNA            complement(28224..28295)
                     /locus_tag="VCF75_000250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28261..28263),aa:Gln,seq:ttg)
     gene            complement(28346..28426)
                     /locus_tag="VCF75_000251"
     tRNA            complement(28346..28426)
                     /locus_tag="VCF75_000251"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28390..28392),aa:Tyr,seq:gta)
     gene            complement(28487..28717)
                     /locus_tag="VCF75_000252"
     CDS             complement(28487..28717)
                     /locus_tag="VCF75_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEP2545847.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            complement(28781..29848)
                     /locus_tag="VCF75_000253"
     CDS             complement(28781..29848)
                     /locus_tag="VCF75_000253"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEP2545848.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            complement(29926..32385)
                     /gene="parC"
                     /locus_tag="VCF75_000254"
     CDS             complement(29926..32385)
                     /gene="parC"
                     /locus_tag="VCF75_000254"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEP2545849.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKEADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAGKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            complement(32476..34425)
                     /gene="parE"
                     /locus_tag="VCF75_000255"
     CDS             complement(32476..34425)
                     /gene="parE"
                     /locus_tag="VCF75_000255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEP2545850.1"
                     /translation="MTKNEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVDSIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGANFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVETGHVYIALPPLYKMSKGKGKTEKIAYAWTDGEL
                     EDLRREFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTINDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            34560..35201
                     /gene="plsY"
                     /locus_tag="VCF75_000256"
     CDS             34560..35201
                     /gene="plsY"
                     /locus_tag="VCF75_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEP2545851.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            complement(35259..36527)
                     /locus_tag="VCF75_000257"
     CDS             complement(35259..36527)
                     /locus_tag="VCF75_000257"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEP2545852.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVEVVAFAQRLGAKVTAEVSPQHFSTTEDLLLTAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(36659..37312)
                     /locus_tag="VCF75_000258"
     CDS             complement(36659..37312)
                     /locus_tag="VCF75_000258"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEP2545853.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPSRENVFK
                     ALQVTPLEKTKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWELFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            complement(37445..38095)
                     /locus_tag="VCF75_000259"
     CDS             complement(37445..38095)
                     /locus_tag="VCF75_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP2545854.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SRQPLIHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            complement(38124..38987)
                     /locus_tag="VCF75_000260"
     CDS             complement(38124..38987)
                     /locus_tag="VCF75_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP2545855.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWETIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            complement(39105..40559)
                     /locus_tag="VCF75_000261"
     CDS             complement(39105..40559)
                     /locus_tag="VCF75_000261"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEP2545856.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKPFAGVPIFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVK
                     RLEDLGFIILGRSNTPEFGFKNISDSSLHEPVNLPKDNTRNAGGSSGGAAALVSSGIS
                     ALAPASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRPLTIAFYQRLNDGSPVSLDTAKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            complement(40756..41385)
                     /locus_tag="VCF75_000262"
     CDS             complement(40756..41385)
                     /locus_tag="VCF75_000262"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEP2545857.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLNAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            complement(41443..42135)
                     /gene="pyrF"
                     /locus_tag="VCF75_000263"
     CDS             complement(41443..42135)
                     /gene="pyrF"
                     /locus_tag="VCF75_000263"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEP2545858.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRYIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            complement(42412..42948)
                     /locus_tag="VCF75_000264"
     CDS             complement(42412..42948)
                     /locus_tag="VCF75_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545859.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     SAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     EAFIKYYQSPKVPTGEFE"
     gene            43170..44075
                     /locus_tag="VCF75_000265"
     CDS             43170..44075
                     /locus_tag="VCF75_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP2545860.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHNKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            complement(44155..44943)
                     /locus_tag="VCF75_000266"
     CDS             complement(44155..44943)
                     /locus_tag="VCF75_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEP2545861.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRTIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKKELSLWRQSIV"
     gene            complement(44936..45649)
                     /gene="deoD"
                     /locus_tag="VCF75_000267"
     CDS             complement(44936..45649)
                     /gene="deoD"
                     /locus_tag="VCF75_000267"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP2545862.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVGLETLIAND"
     gene            complement(46312..47121)
                     /locus_tag="VCF75_000268"
     CDS             complement(46312..47121)
                     /locus_tag="VCF75_000268"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP2545863.1"
                     /translation="MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQHIKEDFKGLVKAILSEL"
     gene            complement(47105..47545)
                     /gene="arsC"
                     /locus_tag="VCF75_000269"
     CDS             complement(47105..47545)
                     /gene="arsC"
                     /locus_tag="VCF75_000269"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEP2545864.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLTDPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYMKEDGESVNPIEVKGEKDVIDD"
     gene            complement(47564..48775)
                     /locus_tag="VCF75_000270"
     CDS             complement(47564..48775)
                     /locus_tag="VCF75_000270"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEP2545865.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     AAGLSVPNMAKIGLGNISRPIPLKTVPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLGHLK"
     gene            complement(48852..49535)
                     /gene="rpiA"
                     /locus_tag="VCF75_000271"
     CDS             complement(48852..49535)
                     /gene="rpiA"
                     /locus_tag="VCF75_000271"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEP2545866.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEGLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEAPTAG"
     gene            49913..52012
                     /locus_tag="VCF75_000272"
     CDS             49913..52012
                     /locus_tag="VCF75_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP2545867.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKESQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGSNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILKEGQLVIKQKDQ"
     gene            complement(52070..52813)
                     /locus_tag="VCF75_000273"
     CDS             complement(52070..52813)
                     /locus_tag="VCF75_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545868.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(52961..53560)
                     /locus_tag="VCF75_000274"
     CDS             complement(52961..53560)
                     /locus_tag="VCF75_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEP2545869.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTTRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            complement(53570..54799)
                     /gene="clpX"
                     /locus_tag="VCF75_000275"
     CDS             complement(53570..54799)
                     /gene="clpX"
                     /locus_tag="VCF75_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEP2545870.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIKILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            complement(55119..55616)
                     /locus_tag="VCF75_000276"
     CDS             complement(55119..55616)
                     /locus_tag="VCF75_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEP2545871.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRMTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNLYDFTVT
                     ALKHK"
     gene            complement(55696..56535)
                     /locus_tag="VCF75_000277"
     CDS             complement(55696..56535)
                     /locus_tag="VCF75_000277"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEP2545872.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            56744..57919
                     /locus_tag="VCF75_000278"
     CDS             56744..57919
                     /locus_tag="VCF75_000278"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEP2545873.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRAKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTADPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMNNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHQRIYHTNDKN"
     gene            57906..59183
                     /locus_tag="VCF75_000279"
     CDS             57906..59183
                     /locus_tag="VCF75_000279"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEP2545874.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(59413..60402)
                     /locus_tag="VCF75_000280"
     CDS             complement(59413..60402)
                     /locus_tag="VCF75_000280"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEP2545875.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(60389..61402)
                     /locus_tag="VCF75_000281"
     CDS             complement(60389..61402)
                     /locus_tag="VCF75_000281"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEP2545876.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILIPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFSHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHPAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQDARDTLISKWQEADIALLYQERWGEN
                     DQP"
     gene            complement(61395..62339)
                     /gene="mvaD"
                     /locus_tag="VCF75_000282"
     CDS             complement(61395..62339)
                     /gene="mvaD"
                     /locus_tag="VCF75_000282"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEP2545877.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISTIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            complement(62321..63199)
                     /gene="mvk"
                     /locus_tag="VCF75_000283"
     CDS             complement(62321..63199)
                     /gene="mvk"
                     /locus_tag="VCF75_000283"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEP2545878.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLIIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
BASE COUNT        19090 a        13346 c        11317 g        19547 t
ORIGIN      
        1 ttattagtca atgatttaga tcgttgacta atcaaatagt tggtaatagt cagtaatcgt
       61 cagttgttct ttttccattt gatttaaatc tttaataatg tttccatctt tcattacaat
      121 cagacgattc ccataagtta atgcatcttc catatggtgg gtaatcatca aagctgttaa
      181 gccatctttt gtcacaaact catctgtcaa ttgcattaaa gattgactcg tcttagggtc
      241 aagggcagcc gtatgttcat ctaataataa gagagctggc tttttcaacg ttgccattaa
      301 taaacttaaa gcctgtcttt gtccaccaga caaaagacct gctggtgttt ctaaatgctt
      361 ttctaggcca tttcctgttc gtttaaccaa atcctcaaaa ctagctaagt gctctgttat
      421 ttttcgagaa gccaaggagc gtttttctcc tctttgacgt gcgattaaga gattttcagc
      481 cactgtcatc cgaggggctg ttcccatttt gggatcttga aagactcgcg ataggtaaag
      541 agctcttttt tctgcaggcc aatgcgtcac atcctgacca agaatcctaa tttgtccccg
      601 tgtcaaactt agcgttcctg caattacatt aaaaagggtt gattttccag ctccatttcc
      661 tcctaaaata gttaaaaaat catgttcata gatagttagt gtcacattgt ccaatattgt
      721 tttagcatct tcaaaaccat tatctacgtc aactgttgca ttgatcaatt caataatttt
      781 tgtcatcgtg ttaacctcac tcccttaaag aagcgctctt ttaccactgg tatcatgagg
      841 cacagtgcta aaacaagagc actaattaat tttaaataac ttgtattaaa acctagcgta
      901 ataactactg aaattaaaaa ttgataaaga atcgagccta ttacaatagc aatcaaacgt
      961 tctaaaagag tcagtccagt tgagtatagt acttcaccaa caattatgct cgccaaacca
     1021 ataacaataa ctccaatccc ttttgagaca tctgcatagc catcttgttg actcactaga
     1081 gcacctgata aagctattaa actattagaa accacaagtc ccataacttc catccaatcc
     1141 gtgttaatac caaaactctt agccatatct ttattgtctc ccgtagcgat ataagcttga
     1201 cctagattag tgtaaaggaa atagatcaaa acgctaataa caatcacaac agttatcaga
     1261 cctgttaaaa ggctatttaa atcaattgaa aaaggtaaac aatcctgaat acgtttatga
     1321 tcatgtaaac ccaagtttgc tcttcccatt accattagca taatggagtt acatgacgtc
     1381 atcaccaaaa ttccagctaa aagtgttggc atttttcctt tcgtatataa cagccctgtt
     1441 agaaaacctg ctaaagcacc tgataacata ccaagtaagg ttgatagaaa tggattccac
     1501 cctaagctaa tagcggtgac cgcaacagct cctcctaaag gaaaagaacc ttctgtagtc
     1561 atgtcaggga agtttaaaat cctaaaggtc aggtagattc ctaatcctaa aactccccat
     1621 ataaggcctt gtgaaactga tgaaataatc atatttttcc tcatatttat attagcgggt
     1681 aactctcgcc agtataaacc ctttctttac attaattttt agatttgttt tgtacttagt
     1741 tcagcaccgt ggcttagtct ttttttcaat cactttgacg aaactaagac agaaaacaaa
     1801 aaagtcgaga atcatgttta aacttactca aaaggcaaga ttttttacgt gtctaaaata
     1861 gagcatttta aaactaaaaa tgataaaaat aacccttaca tcctcaagac gattgtctga
     1921 cttggcatcc aaattcatct cagtgagggt gttttttatg gttttttctt gaccttttat
     1981 atcacatgtc attgtattaa ttctcaataa aagaagctga aggaaaatct tcccttttaa
     2041 tagcaatacc taattctgct gctacattct tattaattag cggttgacca atatccacaa
     2101 ctttgacagg aacatctaca acacgctttc ctttgattaa ttttaacacc tgattagctg
     2161 tttgaactcc caaatcgtac tgattttgag aaatagctgc caaaccacct ttttcaacca
     2221 ttcgatcaac gctagtgtag acaggaatct ttgcagcctt actggttgtc atgactgatg
     2281 aaaatgcact tgctatagta ttgtcttggg ggataaatac cgcgtctact ttacctaaca
     2341 taactgacat cgtggcagga acttcgttag tagaaggaac cgcgtatgaa ataacttgat
     2401 agcccttttt gcgagctaac cgcctaaact ctttaacttg agataaggaa ttatcctcat
     2461 tacttgcata taaaattcca actgttttga catgtggtgt cagctttttc attaaatcaa
     2521 ttgtctgttt aacaggcact ttattagata gaccagttac attagcttca ggttgatcca
     2581 attgcatcac taatctagat cctactggat cagatactgc tgacatgaca actgggatat
     2641 ccttgctggc agctgctaag ccttgcgccg ccggggttgc gataccaatc acgatatcac
     2701 ttcctgattg cactaattgc ctactcattg tttggatttt actctgatcc ccctcagcat
     2761 tcataacctt aatcactaca ttttgttttt gaggcatctg ctttttgagt tgatcttcaa
     2821 tacctcgctt aatatcatca agagcctcat gagtaacgaa ttgtaagata ccaattgtaa
     2881 tgttttgtcg gttagccaaa tcacggtttt cctttgatac tccttttgat aatagagagc
     2941 ctatcactat tacagttaaa acaaggaggg tagctattaa actcttgttt ttcatctgaa
     3001 ttcctcacat ttcatgtcaa tctctttaaa acgttgatga tgagaatact tctcaaaaga
     3061 aaaagcactt agtaatgatt actaggcgct cttgtgaaat ccaatgtcag cgcatagata
     3121 tctttttaga agtactctaa aagtcgtatc tatgccaatt cataaacaca acttatctat
     3181 gccagctttg ccaatttact aagtgttgtg tttttaccat cattgacttc ctttctcgtt
     3241 tttttaacat tataacctaa atcaaaaaaa ttgcaagctc tttcgtccat atttttagtc
     3301 aagtatttgt ggtataatga agagattaga attcagaaag gagaaaccat gtcttttgac
     3361 ggctttttct tacaccattt aaccaacgaa ttaaaagaga acttgcttta cggtcgtatc
     3421 caaaaggtca atcaaccctt tgaacgggaa cttgtcttaa ccattcgtaa ccatcgcaag
     3481 aattataaat tgctattgtc tgcccatcct gtttttggaa gggtacaaat cacccaagca
     3541 gactttcaaa atcctcaggt acctaatacc tttaccatga ttatgcgtaa atacctccag
     3601 ggtgcagtca ttgagcagtt agaacagatt gataatgacc gcattatcga aatcaaggta
     3661 tctaataaaa atgaaatcgg agatgctatt caggcaaccc taatcattga aatcatgggg
     3721 aagcacagca atatcatctt ggtggaccgt gcagaaaata aaatcatcga atccattaaa
     3781 catgttggct tttcacaaaa ctcttaccgc accattttac ctggctctac ctacattgag
     3841 ccccctaaaa cagctgctgt taatcctttt acaattacag atgttcctct atttgaaatc
     3901 ctccaaacac aagaactaac agttaaaagc ttgcaacagc attttcaagg tttggggcga
     3961 gacaccgcaa aagagttggc cgagcttctg accacagaca aattaaaacg gttccgtgag
     4021 ttttttgcta gacctactca ggcaaatctg accacagctt cttttgcgcc tgttcttttc
     4081 tcagatagcc atgcgacgtt tgagacttta tcagagatgc ttgatcactt ttatcaagat
     4141 aaggctgagc gtgaccgtat caatcagcaa gccagtgatt tgattcaccg tgtgcaaact
     4201 gaactggaca aaaatcgcaa caagctaagt aagcaagaag ctgagctgct agccactgaa
     4261 aatgctgaat tattccgtca aaaaggagaa ttattgacca cctacctcag tctggtccca
     4321 aacaaccaag actctgtgat tttagacaat tattatacag gggaaaaaat tgagattgcc
     4381 ttagacaagg ctctgacacc aaatcaaaat gctcaacgtt attttaaaaa ataccaaaag
     4441 ctaaaagaag ctgtcaaaca cttatctgga ttgattgccg ataccaagca aagtattact
     4501 tactttgaga gcgtagacta caatttatct caagcaagta tcgacgatat cgaagatatt
     4561 cgcgaagaat tatatcaggc tggctttttg aaaagtcgcc aaagagacaa acgccataaa
     4621 cgtaaaaagc ctgagcagta cctggcttca gatggaaaaa ccattctcat ggtgggacgc
     4681 aataacttgc aaaatgaaga actcaccttt aaaatggcta aaaaaggaga actctggttc
     4741 catgctaagg atatccctgg aagtcatgtc atcatcaaag acaatcttga cccaagtgac
     4801 gaggttaaaa ctgatgctgc cgaactagct gcttattatt cgaaagctag gttatcaaat
     4861 ctcgttcagg tcgatatgat tgaagctaaa aaattacaca aacctagtgg cgccaagcca
     4921 ggatttgtga cttatactgg tcaaaaaacc ttacgggtta ctcctgacca agccaaaatt
     4981 ctctctatga aattatcctg ataagcaact aaaaaacagc tctcctagga aagttatttc
     5041 ccctaggaga actgtttttt tattctgatt gttggtgctt gatatggtca agaaagtctt
     5101 gcatattcac atcatcaggc acttgtctga ggtaagcggt agccatttgg atagccttgt
     5161 gaaactggcc aaactccctt aataaatagg cataatcctg taaaaactct ggattttctg
     5221 ccaaatcagc cgaaatctca ttataaagag ccaaagcaac ttcttcttgt tccaaggcgt
     5281 ggtaagcctt tgcaatggtc catttggtta agacattatc aatcgtctca cgattaagag
     5341 caataacctc ctcaaaacgc tcagcatcaa aataaagggt cactaaacgc atgagaatct
     5401 cttcgtcatc aacagctact tcctttgctt gaagcaggta attttcagca ttctgcctat
     5461 cgtgtaattc ataagaaagc tgcgatgcta gtaataataa ttgactgtca aatgcatttt
     5521 tacgtaggcc ctgttgaacc aagcgcaaag cttctgacgt cttatgctct tcatgaagac
     5581 tcaaagcata accatattca taacctggat aatctggatt gatggtttcc aactgtttaa
     5641 agtaaagatt ggctttttga taattttctt ggtcatacat gagggtcgcc aattcaaaaa
     5701 cagtttcatc ttcgtattca atggccacgg ccttttccaa gaattcgatg gctgcctcaa
     5761 atttccctaa gctagcatag gctcgtccaa tgcgttgata agtggaaata cctgtcaatt
     5821 caaggatttg acgattgtct aactgtgcat agtaatcaat agcttcttta aaatactgca
     5881 aggacatgtc gatttctgcc aaaccaaaaa tgaccaatgg ctctgagctg atggccactg
     5941 cttgaagcaa tttttcacgc gccacctctg tcagtccttc caggtcatag aggtcagcca
     6001 tgactaagag agctgacaaa taattagggc tatcttttga aaccttatcc aaatacaaga
     6061 aagcttcttc aatggcgtcg tcttctgctg caatctgagc taaatttata ttgagttctg
     6121 ggtaatcatc agctaactgc aaataaatac gcttggcatg tggcaaaaaa cctatacttt
     6181 ctaagtattc tcctaaggcg attaaactat ctgcatcatc ttcttttaaa gctttttgga
     6241 aatatttttc agcgtgcgct aggtcttgct gatccaagga agcaatcatt ttttcactat
     6301 ttaacatggt ccttaacctc ctcgatcacc aatacttctt cgtcatacaa accgctaact
     6361 tttttgtacc agtcaaataa ttctttaatc acgactttga cagaagcgta gattgggatg
     6421 acaaggaaaa caccccagac accaaacata gagcctgcag tcaataacaa gaacataatg
     6481 gtaataggat gaatgctcag cttatttccc aaaacaagcg gtgctacaaa acgcccttcg
     6541 atggtttgct caatcataaa gataacaagc accttaacca acataaatgg gccttgtacc
     6601 atagccataa tcacaacagg tatcatggca aggaaacttc ctaagtaagg tatcatgtta
     6661 agaaagccag caataatccc aaaagtgatg gcatatttca gtcctacgag gctaaacatg
     6721 atagaaaaca taaagccgac aacaattgca accgtaacct gcccttggac atagcctgat
     6781 aattgcttgt taatatctcc caagactcga accataggca cccgtaattt gagaggtaag
     6841 acattcacaa ggccattttt catgtgacta ctgtctcgta aaaaataaaa gagaataaag
     6901 ggcgaaataa taatagccac ggtaatccga gcaatggcac cagcaaaatt accagcccag
     6961 tcaatagcat tttttgaaaa actttgcgcg taatccaaag ccttttgact tgtatgggta
     7021 agcatgtctt ggagttgggg cttgtagctg actaaccatt cattttcaag aagtttattg
     7081 gcttcttcat tgaccttgcc cacgtatttg ggcagatcct caataaaact ggtaagttgg
     7141 gtctgtacca tgggaacaaa gcctgaaatc gcccagacaa aaagtaaggt aatcaaacca
     7201 aaaacaataa aaatagaggt ggtacgattc gggcccaagc gattaataag gtcaactaag
     7261 ggtttggtca gataatacag aatggtcgaa ataaccaagg gcaacatgat gaccgcaaaa
     7321 aaagaaatca ctggcataaa taaaaaagag attttagtaa aaacaaaaat cgttaaaaag
     7381 gctaaaagtg ttattactaa agccatggta gcttgattat ttaaaaacca tttgtaaaat
     7441 aagctatctg ttctagactt gtcaggtatt aagggcatta agatagggtt cctttctttg
     7501 ggaaaatgat aagttttact ggtaacctgg gtaaatcaat aaaaggtcac agatttatcc
     7561 atgagttggt tgttttggtc ataggtaaat ttggcagaga aaaaaggacg gtcttctaaa
     7621 atccatttaa aaatgtcata atcaccttcc caagttggct tttccaacac ttgatcgtaa
     7681 ggtacccatt ccaaggttcc ttctcgtgac tcttcatctg aaatgaggtc tccttcaaat
     7741 cctgttactt taaaaacata ggtataccag tcatgaccag gggtaaattc tggaaaagta
     7801 atgattcctt taaaggccat ctctgtaaca gtcaaatggg tttcctctaa aatctcacga
     7861 cgggcacact catctggggt ctcaccagcc tcgagcttac ccccaactga aatccacttg
     7921 cctttgtgga catcattttc ttttttattg cggtgtaata atagcaaaga atcgccatta
     7981 tcaatgtaac aaatggttgc taattgagtc atctatctct ttcctttcca tctttgtttc
     8041 tattatacca atctctagaa aatatgctat cattttatga gtcaaagtgt gaatacttag
     8101 tcacgctcaa aaccttctaa agatgcctta aaaaagtgta gataagagcc tacaatgaaa
     8161 aacatcaatt taaactatca aaagaacctt gtcatatcaa cgataattga tatgacaagg
     8221 ttctttaatg ttttatttaa tcacttcttg agttttagca tacttagctt ctacagcatc
     8281 tttttctgct ttccaccatg tctcattttc tgtgtaccac ttaatagttt cttccaaacc
     8341 ttctgaaaag tttgtaaatt gtggttccca gccgagttct tcacgcaatt ttgtagaatc
     8401 aatagcgtaa cgaagatcgt gaccagcacg gtcagttacg tgatcataag catcttttgg
     8461 ttgacccatt ttctcaagga taagctcaag aacttccttg ttatttttct cgccgtcggc
     8521 accaataagg tatgtttcac cgatacgacc cttagtcaaa atagcccaga cacctgttga
     8581 atggtcattt gtgtggatcc agtcacggac gtttttacct tcaccataaa gttttggttt
     8641 gattcctgcc aagatatttg taatttgacg tggaatgaat ttttcgatgt gttggtatgg
     8701 tccatagtta tttgaacagt tcgaaatggt cgctttcaca ccgaaggaac gtacccatgc
     8761 cttaacgata aggtcagaag ctgccttagt tgatgagtaa ggtgatgatg gattgtattt
     8821 tgtttcagca gtgaattttt cacctggtcc ttcaccctgt cctggaaggt cttcacgaag
     8881 tggaagatct ccataaactt catcagttga cacgtggtgg aaacggatat cgtatttacg
     8941 agctgcttca agcaaagtgt aagttccgat aaagtttgta tggataaatg gacttggatc
     9001 ttccaatgag ttatcgttgt ggctctcagc cgcatagtga acaatagcat ccgttttggc
     9061 agccaatttg tctaccaatt cagcgtcagc gatatcacca acaactaact caacacgatc
     9121 accaagaata gcttcaatgt tagcacggtt acctgcatat gtcaatttat caaggacagt
     9181 tacatgaaca tctgggtggt tattgtagac atagtgtaca aagttagaac cgatgaaacc
     9241 agctccacca gtcacgataa tatttttata cataagatat aaagggacct ttcgtttccg
     9301 aaaaaatcta ttaaaagtaa taactctaac aaagacgttt taggcctaag aaaacatcgc
     9361 tttctaagat ttgagtccca tgttcagctc aaagagagaa actgaacttt cctttcatca
     9421 tttttagttg ttacttgtta cctgaagtag agaagaataa ctcccctcat atccacctca
     9481 aatgattaat cccaatcatc tgagtatgtt gtttccctat aggtcttttg gtttcagtgg
     9541 tttgacatca ctaagaagag gatggttttt atctgcttca gaaacttctg cagctgctag
     9601 attttcccaa gtaatcccaa gacttgggtc agcatagttt accaaagcat acttaggttt
     9661 caagtcaaga gcccagtagt cattgacaag ataactgtaa gagacagtct ctgaaagaac
     9721 ttgaaagcca ttagcaaccc ctctaggaac aaacattcct tttgaagcat caatcactgt
     9781 ctggtagacg tttccaaaag tctctccctc acgaagatca acccaagctc ctaaaacctt
     9841 gccatcatca gcaactgaga tgtatttatc ccaaggttca gcatgaagtc cacgcagcac
     9901 atgctgacgt gaaaaagaaa cgttgttttg tagttttcct tcctcaaaaa aacgttctgg
     9961 aaagccaatt ggcaacattt tttctttttg gaaattttct ttaaaccagc cacgattatc
    10021 cccacgaaca ggaatatcaa actctagtaa gcctgggatt tctttgattt ctcggcaagc
    10081 caatggtttg tcaaaaaaag tttctgtcat taagcttctc caatcaaacg aagcaagtat
    10141 tgcccgtatt cgtttttctt taaagattgc gccaatttat ggacatcttc tttactgatg
    10201 taacccatgc gataggcaat ttcttccaga tttgccactt gagcgttttg caaacgttga
    10261 acagtttcaa tatattgagc cgcttcaagt agactttcat gcgttccagt gtctagccat
    10321 gcaaaaccac gccccatcaa ttcaacggag aggtcaccac gatccaagta agccttgttg
    10381 acgtctgtaa tttccaactc accacgcgcg cttggtttaa tgtttttagc aatctctacc
    10441 acgtcattgt catagaaata aagtccagtc actgcaaagt gggacttagg cacttctggt
    10501 ttttcttcga tagagatagc attcatgttc tcatcaaatt ccaccactcc aaagcgctca
    10561 gggtctttca cttgataacc aaaaacggtg gctccttttt ctttggcagc tgccttttga
    10621 agcattttag tgaggccatt accgtggtaa atattatctc ctaaaataag agcaacacgg
    10681 tcatcaccga taaactcctc tccaataatg aaagcttgtg ctaaaccatc tgggcttggt
    10741 tgttccttat aggataagct aatgccaaac tctgacccat ctcctagtaa ttcttcgaaa
    10801 cgaggaagat cttggggggt tgaaatgatt aaaatatctt taatccctgc caacatcaag
    10861 gttgacaatg ggtaataaat cattggtttg tcataaatgg gcatcaactg tttagatgcg
    10921 gcacgggtca gtggatacaa gcgtgtccct gaacctcctg caagaataat acctttcata
    10981 gtagtgctcc tattcttcta tataccctat cattctatca ttttttgaaa gcagtgtcac
    11041 gctttaccag cagaccacaa aaggtaaggc tcaggggaat agtcgctagg cgttaaaagt
    11101 ccttcgtgtc ctagcaataa ttgagctaat tcatagccaa tcaaaggtcc aactgttaat
    11161 cccgatgacc ctaaaccact agctgtataa aggttcttca atcctggaac ctgaccataa
    11221 aaaggagaat aatcactggt ataagctctt attcctacac gacacgtttt ttgggtagct
    11281 tcttttaata acggcaaata agtaagagct tgcatttcaa gtttttctaa aacctgccaa
    11341 tcaggctcca aatcataacc tttatcattt tcgtgactcg caccaacaga aatcttacct
    11401 tggttaaaag gaataagatc tatttcacct tctggcataa caactggata agtgtcgctg
    11461 atgatgtgat gaacgtcata atccaagagt tgtccctttt gtggtcgcac atcaacctga
    11521 tagcctaaag gcctcaataa gtcaggtagc catgctcctg ctgccagaat gacctgatca
    11581 aaataatgac cagcaatctc atacccactt ccttgctgtt tcagagtgac tttttggcga
    11641 ataactggat aaccactggc cttgagtaac gtctcacata aagcggctcc ctccactcta
    11701 gcagctcctg aagcgtacag acagttatca aagcctatca atcctttaaa atcattcccc
    11761 agttctttac gatttttaat ggctaactca ccaataatcg gtgattctac ctttcttgct
    11821 aacgctaatt cataaagatc tcttagtttt tcttcctgct tttttaaaac atagatgcca
    11881 ttttgctgat aaaaatctgt tgcaaaacca tcttctttga ggtcattgat taattgctga
    11941 taaaagtccg ctcctaaacg agccatgcga taccaaactt tattgcgacg ctttgaaaac
    12001 caaggactaa taataccagc agcagcctta gtggcttgtc cttgaccatg gtcaaaaatg
    12061 gtaacttctt tttgaccact ttgttgtaaa taataagcgg cggttgatcc tacaatcccc
    12121 gctccaacaa ttgctatttt cattcctggt atccttttat cttattttaa gaccttaaca
    12181 gcctataaat ggctaaatgg attggtcgag gctttgcttg aaataatgct gacatcccaa
    12241 ccgttctctt ctttccaccc ttggagtttc tcctttaatt tctccgtaaa aagcacctca
    12301 atatgatgcc ccgggtcaac cgcaaatagt ccttctgtca gcatctcctg tgctgtatgg
    12361 taatagatat cccctgtaat ataaacgtct gcgccttttt gcacagcatc ctgataaaat
    12421 tcaccaccac ttccgccaca gatagcaatc tttgaaatca aagggttttc cttatcgtaa
    12481 cggataagtc gcacggtatc taaatcaaac acccttttga ccttacttgc taactcttct
    12541 aacgcttgtt ctttaactgt tccaatacga ccaatgccaa agccttcttt agtttcagac
    12601 aagtaagttg cttccttgat ttctagcagg tcacaaaacc aatcatttag tccgcctggt
    12661 acaatgtcaa tattggtatg gctcacatag atagaaatgt catgcttgac caaatctaga
    12721 aggatatcac gctgagggga tgaaaccaga tctttaagcg gtttaaagat gggagcatgc
    12781 ttagttataa tgagatcaac ttcatttttg atggcttctg ctactgttga ctctcggata
    12841 tctaaagtaa tcataacctt acgaatgtct ttatccaatg aacccatttg aagaccttta
    12901 acgtctcctt ccatagataa atcaagtggg caaaaggctt cataggcatc aattagggtt
    12961 ttagctttca tgaacaatca cctcttttat ttgttgtatt tttgtcaaga gaagctgacg
    13021 ctcttgagtt ttttcttctg ggatgcagga caaggcataa gctaacttat ccatttctcg
    13081 ttgccacttc tctttaaaaa cgactgattt ttcctgagaa aggtatggac caaaacgcaa
    13141 ttctgtcgcg gataaggcct tttcaccatg ctcagcaacg atgatttcat aatatttgtc
    13201 attttcagcc atgatggttt ctgcaacaat tttaaaagcg ttaaccgata accaagcccg
    13261 taaatcatcc tcacgattgt tgggctgtaa aaccagacgt tcaatgccct gcaatttttc
    13321 cttacctgcc tctaaaatat cagcaatcaa acggccaccc atgccacaaa tggtaatggc
    13381 tgtcacatca tcagcttctt caaaggctgc aaggccattg gctaagcgca cctgaatatg
    13441 ctcggcaagg ccagactgtg tgacattttt aagagcagat tcgtagggac ccctaaccac
    13501 ttcgccagca atggctgctg aaatctgatt agtttctacc aaaaatatgg gaagataggc
    13561 atgatcactg ccaacatcta ataatttgac accttttgga acataggccg caacttgggc
    13621 taaacggttt gataattgac tatccatctt gatttctttc taaaacgtaa tggttctatt
    13681 atagcaaaat tacctccata aaaaaagaaa accaaaggct aggctttcga ttttccataa
    13741 gtattttgat agtacttgca gtaagactga acgggacaaa tctcacattt tggttttttg
    13801 gctaaacaat ggtagcgtcc aaaaaaaatc aatcgatggt gagtaataat ccaatctttc
    13861 tttggaattt tagccatcaa atctgcttca atctgcttga catcggcatc tggtgatgaa
    13921 atattgagcc tctttgagac tctagccaca tgagtatcaa ccgcaatagc tggaacacca
    13981 taaacctctg ccaataccac attggctgtt tttcggccaa cacccggtaa actttcaagc
    14041 tctttgtggg tttttggcac ttgaccctta aaatcatcac gaatagcctg agcagtttta
    14101 atgatatttt tggccttatt tttatagagt ccaatcgttc tcagagcatt ttcaacatca
    14161 gaaagttcag caaaagctaa gtcttctatt tctggataag actgccatag tccaggagtt
    14221 accttattga cggccttatc tgtcgtttga gctgataaaa tcaccgcaat caacaattga
    14281 aaaggtgttt cccagtctaa ctctccctta gcctcaggaa acatttgacc aatgatggtc
    14341 aggacttttg ctaatcttgc ttttccaatt ctcatgaatc actccataaa ttcatcgcag
    14401 atagaaaatc ctctgatatc gtcacttgag aaagatcttc gccttctcta acacgtctac
    14461 gttcttctac ttgtcgaaga ttaacaatac cttcttttcg ccaattacgt aagatagcct
    14521 ggatatattt ccaatttgtt ttgccgttga agacagcttc ttttaaagct tctcgtatca
    14581 aatcaggatc tgttttatca tcacgaagcg ttttttcaag atcttctaac tcaaaaggac
    14641 tcaagaatcg tcctaattca cgttcaaatt catcaactaa tcgtttgaaa tggtttggtg
    14701 tttcttgttt gtcaatttca gttgccgttt gactgacaaa caattgatct aacttggcta
    14761 acacaggact cgcatcaaaa ataatttcaa tttctccagt aagttcaatt gtccgcatat
    14821 ctaaaagccc ttgactagtc aaagatgaaa tgattttatt aatatcagca actgattttc
    14881 caagcgcatg agcaatttga ctgggtgcta agtcatctcg ctttgtcgta ttttgaagat
    14941 ggaaaaactg ccagaccaaa aaatcatctg agctcttaaa aagatctttg taatgaaaaa
    15001 gcaaggcact tgggataacc aaattacctg acttataatg ttctaaaaaa ctcataatcc
    15061 ctcaatcaaa aggctaaaat cgaatctata gcctttacta ctttaaatcc cctaatcaga
    15121 tgctgattaa tgtgcttacc caggaaactg cattaagact ttatagtcat agttcctaat
    15181 agcatggcga ccatttaaac cttctaattc aataaggaag gcacaaccag ctacaatacc
    15241 accaagtttt tcaatcattt caatagttgc ttttaccgta ccgccagtcg ctagtaaatc
    15301 atcgacaatc aaaacacgtt gtccaggctt aatagcatct gcatgcatag ttaaggtatc
    15361 aagaccatat tctttttcgt aatcagcaga gacaacatct cttggcagtt ttcctggttt
    15421 acgaacggga gcaaaaccaa ttcctaactc aactgcaaca ggacaaccaa tgataaaacc
    15481 acgcgcttca ggtccaacaa ccatatcaat atctttatca caagcgtact gtgctatttc
    15541 acgaatagca tagctatatg ctttaccgtc agccattaaa ggtgagatat ctcgaaacgt
    15601 aataccagct ttagggtaat ctttgatcga tgcaatgtaa tttgttaaat ccatagttaa
    15661 ttctttctag tggcaaattg ccaaatataa aatactcttc attatatcac gaaacatatt
    15721 tttcgtaaaa taagacttct gacattattg tttatctgat acttcattcg taactacctc
    15781 aataactact gtcaactgct ttacttattg atcgtttgat ttcaccaaat aatcatacat
    15841 ctctttcggg ctagccaaag ccatgatttc ttgaaatttc accaattctt ttaagtcttg
    15901 gtaaatgtgg ctctctgaaa tctctcgttt ttgcgcttgg gggttaaccg tcatcagccc
    15961 atcgtcaatc gtcacaaaag acaactcttc aaaaatttga atcaacttaa tcagtaaaag
    16021 tttttctatg tttaaatagt gactcaactc tgtcaattta tgccgtaaat caaattcagg
    16081 aaactgatag atggttttgt agagcttggc aaactgctct ctgctgccaa atcctgttaa
    16141 ataataagga tgtttcatct gatttttaaa atagatggcg tcaaaatcct tgtgaacaaa
    16201 ctggttgcgc caggctttga aatcttcagg gatgtcatta atcagaatga ctctggcatt
    16261 tgggtcttct tcaatagtcg gaataccttc aggaactttg gctgtttttg tccttaagtc
    16321 aagaagttgc actccgtcaa cacgagcatc tactaacatg aattggagac tcgtgttccc
    16381 attccaatgg ttgacagata aggtaactgc taattctaaa ccagtagctt gtcggaactc
    16441 ttgcagttga cttccttgac caaaagccag tacgtcaaag gaagctttgc cctgactgac
    16501 tttaaattta agatggctct ggtcttgccc tatggtacgc gcttgactaa cccttatgtc
    16561 cttgacataa aagaccggct tttgatgatc catcccataa ggggctaatt tatctaaact
    16621 ctttaaaata tcaaggctaa gatcgtccaa agacagtctt tcatcaatag ttaacgtatt
    16681 tttggcagtc tgatctaagc cacgttctat cacaaactga caaagaaagt ctgagagagc
    16741 ttccaagttg tcaacgggca gggtcattcc tgctgcacca gcatgtcccc caaaggctgt
    16801 aaataactca cgcttgccat ttaaggcttc aaaaatatta atagcttcta aacttctggc
    16861 cgagcctttt gcaaagccat tatcaatagt caaaacgacg accgtttgtc caatagtttc
    16921 catgatacga ccagcgacaa tccccagaac accaggatgc cagccagctt gtgccaagac
    16981 ttgaacaggt ttttgaggat caaccatagc catcgcttga tcaaaaatgt cttggactaa
    17041 ggccttacgc tcttcatttt ttttcttaat catcaaggca atcgcttgag cttcttggtc
    17101 atcaaagccc gtcagtagct caatggctgg attagggtca tctaagcgcc ctaaagcatt
    17161 taattgcgga gcaatctgaa agccaatggc gtcttcatta aaatgttcca aatcaatagg
    17221 agacaaggac atcaactcct gcaagcccac tctttcggtg tgtttgagca tggccagacc
    17281 atttttgact aaaacacggt tttcacctgt caagcttacc atatcggcaa tggttccaat
    17341 agctaccaag tctagacagt ctgttggtaa actttctagc aaagctgttg ccaatttaaa
    17401 ggcaacgcca cagccagcta agtgtttaaa gggataatca gcatcaggat gctcaggatg
    17461 aatgatggca aaggcttctg gcaattcttc aggcaggcta tgatggtcag ttacaatgac
    17521 atcaacttcc tgttcttgag catatctaat cgcttcatgt ccggccacgc cattatcaac
    17581 cgtgataatt agggacacag cttcctgctc aataaaatat ttatagacgc tctggttggg
    17641 gccatagcca tctgtaaagc ggttgggaag ataaaccaaa ggctctgctc ccatcatgtc
    17701 tagggtttct ttgacaatag aagccgacgt catgccatca gcatcatagt cgccgtaaat
    17761 gagaattcgt tcgccctctt caatagcttg acgaattctt ggaacagctt ttgccatgtc
    17821 atgtagcaag taaggatcat gcagttgaga caagtccgct gttaaaaact catctaaggc
    17881 ttcttcagtc cttatccctc gatcataaat caactgagcg gcggtctggg ttagaccttt
    17941 tgtctttgct agcttaaaga agccatcatc tggcttcttg tcttttattt tccagctata
    18001 tttggattta atcatttata attaaatacc tttcttgcaa gataatcaga taatttaggg
    18061 aaaagggtgt aaaattgatg ggtcaccgct aaactaaagg gcaaattcaa ttctcgttta
    18121 tttttcccga taatagaaac caaacgctta gccacttgat ttggttggag agtaaattta
    18181 ccaacgcttt ccaaataatg tccagacggg tcagcttggt taaaaaactt ggtggcaatg
    18241 ggacctggat tcacggtggt cacgtaaacc cctttatccg ctaattctaa gcgaagggca
    18301 ttggaaaatc cgataagggc aaacttggtg gctgaataaa tgctcgattt ggctgacgca
    18361 atcaagcctg ccatggacac aatattaata agatgacctt gcccctgctc tgccattttc
    18421 tgaccaatca agcaagcaaa gtgaatgctc gctagggtgt taacctgaaa catatcagct
    18481 atttctttgg cagaaaactc ttcaaagcct ttgaaagctc cataaccagc attattaatc
    18541 aagacatcaa tacggccata gcgctggtaa atctgggcga ctattttctc aatggctact
    18601 ggattggtaa tatccaactc aaggcattct ttgttgtcaa tatgctgata acagtgttct
    18661 aggcgttctt tgttacgtcc taataaaatc aagctgtctt ccttgggtaa ctgcttaaca
    18721 attgcctgag ccagtcctcc agaagctccc gtgataacaa tgattctttg tgccattaaa
    18781 taatcacttc ctctaaatct tgtaccattt taacattttc aaatatagtg gcggcatctt
    18841 tttccatctg gcgacaatca cgccctaaaa agcgagctga tacatgattg agcaagaggc
    18901 gttttgcgcc tgcttcatga gcgatttgtg ctgcctgcat gttggtagaa tgcccgtgat
    18961 ttctcgcaat ccgctcgtca cccttaccat aggtggattc atgaaccaaa acatctgcat
    19021 ctttagcgag tttaacactg gcagacgttt tgcgcgtatc gccgataatg gtgatgattt
    19081 tgcctttttt aggggcagaa atataatctt tggcacaaat caggcggccg tcttccaact
    19141 caacgtcttg accattttta attttgccaa agagtggtcc aaaaggaaca cccgctgctt
    19201 ttaaggcttc tgcgtctagg gttccttcta agtctttttg aactactcga taacccatgc
    19261 aaaaaatggt atgcgccaga cgctctgcat agacctcaaa cttatctgtt tccattattt
    19321 tacctaatga tttgtcatca aattcatgaa aatgaatctg ataaggtaca cgcgctccag
    19381 aaacttttag actagttaac acgtaagttt taatgccaat tggtccataa atatcaagat
    19441 ctgtctgttc ttcactagct tgaaaagacc gacttgataa aaatcctggt aatccaaaaa
    19501 tgtggtctcc atgcaaatga gtaatgaaaa tctttcttat ttttcgtggc ttaatcgtcg
    19561 tttctaaaat ttgtctttgc gtcccttcac cgcaatcaaa catccagact tcattgattt
    19621 cgtctagtaa tttcaaggca agacttgaca cattgcgctg cttggctggt tgaccagctc
    19681 cagttcctaa aaattgtaat tccattcaat ctattctttc tattgggtga tgtaaatcat
    19741 aaattctcgt tgggcaaaat cataatactt ttgaccagaa aatgtttggc taagagccca
    19801 taactcctct acattaaacc ccttaagcat ggtgataccg tttgccagac gataaatctt
    19861 cgacaagagt gtgtcatcag gcgtaatgtc ttcacattct ccttgctcaa actgcttagc
    19921 ctgcatttta agctggcctt tgtcttcata aaccatatat tgtgggaaaa tttgtgttaa
    19981 ttcaaagaag agttcgggtt tcttaggctc tgctttttca gaaaatacca atgcaacttc
    20041 ttggtcattt tggtaagcaa aaccataagc ttttccagac aaattgaggc gtacaaaggg
    20101 cttttcttcc tcaaaagatg gttggtcagt aaaaagcccc aaagccttag aaagattaaa
    20161 ataataatca gtcgcagctt gcggtgactg tttctggtag atttcagcaa agtaggcatt
    20221 aataacactt ggtggtggtg tgataaaagc tagtttttgc tggtctgtca aactggctaa
    20281 cagatggttt aggtagttgg tatccagact agtaaatcca ccataggttt ttgctaaatc
    20341 aatataatta ttcataaaaa tcgtcaagtc tccattgctg ttttggggaa atgtagcccg
    20401 aaatatcctc tatttcttgg tcaaacgtgt agtggtccaa aagagcaact cggttcaaat
    20461 catacagctt gtacgccttg tcttgatgca ctttgattct aaaaggaaca aactggtctc
    20521 gaatttggtc aatgattaaa cgtctcaaga gaattttgct atcctttgaa cgggctgaaa
    20581 tcctgatatt tggaaaagca gtagctgtga attgttctgc tatatccacc ttgttataaa
    20641 tagctaaacg tggaatatta agcatgtcta gctcttttag aaggtttaaa accacttttt
    20701 cttgctcact atgattaggg tcgctagcgt caatcacatg taacagcaaa tccacatatt
    20761 tgctttcttc tagggttgat ttaaaagcag ccactaattc cgttggcaag tcttgaataa
    20821 agcctacggt gtctgttaag gttgcttgaa attgattttc taaatagagt tgctttgtag
    20881 ttgcatctaa ggtagcaaat aattcattgg cttcatagtg gctgtcatct gtcaacaagt
    20941 tcataatggt tgacttgcca gcattggtgt agccaatcaa gccaatttta aaggtatctg
    21001 aacccacacg acgatctcta atggtttgac gatttttctc tacctgagtt aattggcgtt
    21061 caatatcagc aatctgatga cgaatcgaac ggcgatttaa ctctagttgg ctttcacctg
    21121 gtccacggct cccaatacca cctgcttgac gacttaacat gatgccctgt ccaaccaaac
    21181 gaggtaacat atatttcaat tgggcaaggt gtacttgtaa tttgccctca tggcttctgg
    21241 cacgcatggc aaaaatatct aagattaact gcatacggtc aatgactttt acctctaaaa
    21301 cagcttctag attagcattt tgcctagcag tcaaccgatt gttcacaata actgcatcaa
    21361 tctcatcagc ttccacaatc gccttaatct catccagttt ccctgagccg ataaaggttt
    21421 tgctgtcgta acgttctcgt ttttggctga aacttgccat aactttgacg ccagctgtct
    21481 tggcaagatt agctaattct gtcatggaca tgtcaaaatg ttctgttgtc tgaagctcaa
    21541 ctcctaaaag aatcactctt tcttgctgac gctttgtctc aaccatcacc tttaccaatc
    21601 ctccaaattc cgtaaattct attataacat atcttcaaca gattctgcta attttgcgtc
    21661 atgacttctc tttctgacca agaaaatccc tgaccctatc atgtactacc tgtgggtaat
    21721 caggagccgt tatggcagtg aagctaacag ccattcgatt gcggaaccaa gttagctgtc
    21781 gcttggcaaa acgtctcgtg ttttgtttta actgatcact agcttctgcc aaagtcattt
    21841 cacctacaaa ataggggaat aattccttat aacctatgcc acgactagcc tgaacagtcg
    21901 ggtaatgctc atataaccat tttgcttctt ccaacaagcc gttttcaagc atatgatcca
    21961 cgcgctgatt gatacggtca tagatgactt ggcggtcatc attcaagccg ataataagcg
    22021 gctcataagc cgtctcggca ttctctaatt catcagcaaa ctgagccaat tcaagggctc
    22081 ggatcgcccg cctgcgattc acctgctcaa tggtaatgtt attgacttgc aagcgttcat
    22141 acaagtcgtg atcgtctagt tgctccaact cattccgata ggcttttaca gcctcttggt
    22201 ctactgttcc tcctaagtgg taaccttcga gtaaactttg taagtaaaga ccagttccac
    22261 ctacaataat tggtaatttc ccacgactaa caatgtctga aatagctttt tgagcatctt
    22321 ggacaaactc gtaggccgaa taagactccg tcacctcacg aatatcaatt aagtgatgaa
    22381 ccgctgcttc ttgctcttct tgagtggcct tggctgttcc aatatccaac tgtcgataaa
    22441 cttgctggct atctcctgaa ataatttctc cattaaaagc cttggctaaa ctaatcccaa
    22501 gtgctgtttt tccaaccgct gtcggaccaa caatcacaac tatttttatt tttgtcattt
    22561 tcttgtcttt ctattagaaa agcgtgtcca aaaacgcctt ttacttgaaa attagcttat
    22621 ttttcgctac catatattat aacataagat taaggaccac tgaggtcctt cactagaaat
    22681 ggagaataat catggttaag aaatatcaat ttgttaaagg tcttgctact ggcgtcctag
    22741 caactgctgc tactgttgct ggtgctgtct ttgctgtaaa aaaaacaatc attgaccctg
    22801 aagaagaaaa agctgctttc attgaagaaa accgcaaaaa agctgcgcgt cgtcgtgtag
    22861 ctcgctaacg tgacaatgga tcaatttaaa agagactagc acattaaagt gccggcctct
    22921 tttttagtaa tctcgataac tagttagtag taactgtctc ttgatgagcc aagatgttag
    22981 ctgctttact tattttgaaa tagaaatctc aggtaagccc cataaacata cgtgactaaa
    23041 gctgtcgcca acaatacctc tcctagcaaa agaacctgcc aacctaaacc ttgccaacct
    23101 aaaaaagata aggtcatttt taaggaagtg gcactaatca caaacggaaa ggtaaaagct
    23161 gaaaaacctg ggttaaaggg acgattgagt aagcgaggaa gctgtacaac cacaaaagca
    23221 tataacagtt gtgagctgat gagaagaaag ataaccattc ctacttgtgg tctaggaaag
    23281 gttgctaaat aagacgctga aagtaaggac agaggggcac aaaacgttga aatattgggc
    23341 attactgctt gacctaaacc aatcccataa gtctttttgg ccataaaagg taaaatcaca
    23401 gcagttaaga gaagacaaac ccaaaaaatc acctgtccta ataaaaactg cctactagca
    23461 ggtgctgtta atgccgccat tgcaattcca acaaaaagca cggaccagga aggagaaacg
    23521 ttatcccatg aaaaagaaaa gacaaaacga tactggtaat aagcaatcag gacaagattt
    23581 ccccaaaaga atagccacca agtgagaaag cctaaccaac accctcctgt cactttgaga
    23641 aagagtgaag acaacaacat ccctgtcata aaaaaagttg gaaaaacaga ggcaattaga
    23701 ggttgtcgta actgcatttt ggtatcgtta aggttcctaa ccatgccaac aagcaagata
    23761 ccatagataa aaagagctgc cagaattccc aaataattaa aaatcgaaac ataagtagcc
    23821 aacaaattgc caaatgacaa cgttcctaat gccaatcctg acatgaccaa tggtgggttt
    23881 ttaaggtgtt tcataagtgt ccttttctgc ctattaacag ctatttttag tgacataatc
    23941 ttgtttattt tatcacattt cgagcatttt tgacaccacc cgcgaaagaa aatttaaggc
    24001 tcttgccatt gtttttttac attctcgcaa aactagcaaa ctgcttaaaa agctaaggag
    24061 taaccactct tctctagaaa aatgaaagag tagagaagac ttggtctgat ctttacttct
    24121 tctttttgat ttagtgagct gcattaagca cttgttgaac agctgaatct tccacaattt
    24181 ttgccataaa ggctgccaga tgcggataat tctcgtaaga ttttggtgtt ttttgagacc
    24241 atagcgccat ggcgtaagca taagcatctg caatggtttg ttgatctcga taaacatgcc
    24301 cactctcccc aataagacca tctaaatgcg tcatagcaat acccatccgc ttgtaagaag
    24361 cttgtttgac tttttcaagc gcttgattgt cttgagaaat agtgtagcga tgagggttga
    24421 acattggcct aaaaacaaag ggaaaatcac ctgttaagta gtccatgatt tcgttgaacc
    24481 gactgtctgc caaaagatta tcttggggaa tgagctgaga tttcagggtt tgtttcgcta
    24541 taaagggcaa gataatacct gcctgtgtga caatttgacc atcatcaagc tgtaaagctt
    24601 aaaccattcc caaaggattg atggtaaggc agtcttcaga atccagtttt accttctcta
    24661 cctcgtaata cagaaactca caattctttt gaaaaggttc tagacagagt tggtgttgac
    24721 gtcttcgctg ttgatggctc tgcatgacaa aatgagcttg gtgtccacgt tcacttgtat
    24781 cttgttgcaa agtctttgca atttggtctc atcatctgta tcacaaaaaa caaaccagtt
    24841 tcctgatttg ttttgagata ttagacacct acaaattctg gcaaggattc tccgatagct
    24901 tctaaacgtt ctgcccattc ttgacgtgat aaaccatttt cttgcacata gcgattacgt
    24961 tcatgtgcac gacattcagg cgagcaccct cgaacatact tcgtttcgtt ttcttctgaa
    25021 gcaaaaattt gtttgttaca aaacggattt gcacaattga cataacgttc acaaggtgtt
    25081 ccatcaaaat aatctttact aatgacagtt ggattaacat gattaattgg caccgagata
    25141 cgatcatcaa aaacatacat cgcaccatcc caaagttcac cttgaacttc cggatctttt
    25201 ccatatgttg caataccacc gtgtaattgt ccaacatctt taaagccttc tcgaaccatc
    25261 caacccgaga atttctcaca gcgaacacca cccgtacagt acacgacaac acgtttttcc
    25321 ataaacttat ctttgttgtc acgtacccat tgtggtaact cacggaaatt gcgaatatca
    25381 ggacgaattg ctccacggaa gtgacctaag tcatactcat aatcattacg cgtatctaaa
    25441 acaacagtat cttcatcaag caaggcttct ttaaattgtt taggatttaa atactcacct
    25501 gttgtttcca aaggattaat atcactatca aagttattgt cttctaaccc aaggtgcaca
    25561 atctctttct tataacgaac aaacatttta cggaaagctt gttgattttc ctcgtcaatc
    25621 ttgaaccaaa gatcagcaaa acgctcatcg ctgtggaccc aatccatata cttctgagtt
    25681 gtttcataat ctccagaaac agttccatta atcccctcat ccgcaatcaa aatacgacct
    25741 ttaagcccaa tagatttaca aaattctaga tgtttcgcag cgtactcttg cgcattttca
    25801 attgaaacat acttataata gagtaaaact ctgatttttt ctgacatttt ttctccttca
    25861 ttgttattca gttacttcct attatatcta ggaaaaacaa gtctaggcaa gccatttgtt
    25921 tgtctttttt ttcacaaaat agatctcaat ttatttttga tgagatggca atccattact
    25981 cctttaagca aatcattaga agctttattt agaaaatgct aagataaaac aaggaggcca
    26041 aaatgactta tgaactttgt ttagaatatg ggacttatcc gttgtcacgt gttgatgctt
    26101 actggggaga agatcaaaat cccccaacat tcattcaaga agatcgactg ctttgccata
    26161 aactagaaac tatgaatcac ctgttccatg atttatttgt aactattgaa tcacaattcc
    26221 attatgttgg ttttaacatg cctgaaaaaa gagcgcagat tcggatacta tatcaagaag
    26281 tagctactat attaaaatgc aaatataagg attatccaat taagattgag acgtttcttc
    26341 tttaaaaatc taatactaaa aaactaactg tgcttagaca gatgacacag ctagtttttt
    26401 aggtaaaact cttcttaaat tttaaaacgt ttcgaaaaat cactaaatgc tttaatagca
    26461 cttgctaaaa atggtttcac catctcatca ataattaacc ttgattactt acaatcttat
    26521 gaatctgagt caaataaaat tcaggctgtg gcattacaga gacatttaaa acctatactc
    26581 atcgttttca aggtcagaaa gagaatgatt ttgaaaaggt tcgtcaactc ttctagataa
    26641 ttttggacaa attttgatca agaaacaaaa agagcctatc aaataaggct tttttatatg
    26701 atttctatcc accttgaggg aatcgaaccc ccatctcaag aaccggaatc ttacgtgata
    26761 tccattacac taaaggtggt cacaactttg tcaataccct tatagtatat cataaaatga
    26821 aataatatga aataactttt ctaaccagaa gacaataaaa agttgaggaa aacctcaact
    26881 ttttagattt attacaattc aatatcaccg aaaagatcag ccattgagaa tccagtttgt
    26941 gtttctggga gttcgtagtc acgtcttgat tcacgttttg gacgacgtgg gcgtgattga
    27001 cgtttttctt ctttgttgtc tccttcagct tgcgctgggc gttcttcaag agctttgatt
    27061 gataatgata cgcgctcatc agctgcattc acttcaagaa ctttaactgt tacttcttgt
    27121 cctacagaaa gtacatcttt tggattttca acacgtttgt gtgaaatttg tgaaatatga
    27181 acaagtccat caatacctgg taatacttca acaaaagcac cgaagtcagt caagcgtttt
    27241 actttacctt caacaacatc accttgagca agtttttgtt caacgccgtc ccatggtcca
    27301 ggtgttgttg ctttaagtga aagtgacaca cgaccagctt cttcgtcaat tgaaagaact
    27361 ttaacttcaa cttcttcacc aactgaaaca actgatttag gtgacacgtt acgttcgtga
    27421 gacaattcag tcacgtgaac aagtccgtca acaccaccaa ggtcaatgaa agcaccaaaa
    27481 cttgttaaac gcgctacagt acctgtaacg actgcacctt ctgaaatctt agagaacact
    27541 tcagcacgag cttctttagc ggcctcttcg ataacttcac gacgtgatag gatgaaacgg
    27601 ttttctgctg catcaacttc tttgatttta gcatcaaact cttgtccaac aaatttttca
    27661 gtgttgcgaa caaaacgagt gtcaatcatt gaagcaggga taaatccacg aagtccttca
    27721 aattcaactg aaagaccacc tttaacagca cgggtacctt taacagtaac aacttcgcct
    27781 tcacgaccaa caagtttgtc ccaggcttta cgagcttcca aacgtttttt agatactagg
    27841 aaagtaactg tatcagtatc tttacctact acttgacgaa gaacaagtac ttcaactgtg
    27901 tcgccagctt taacaaagtc gttaatatca gcatcgcggt cgttagtcaa ttcacgaagt
    27961 gtcaagacac cttcaacacc tgttccttcg ataacaacgt ttgcttgacc gttatcaaca
    28021 gttaaaactt ccgcagtgac aacatcacca gggttcactt cgctaacact gtttagcaaa
    28081 tcttcaaatt cattcattct aaaaaatcct ccaacaaaag ctagccaatg ctagcttttg
    28141 ataacaatat attttctcaa ggtaacccgc aacgacaaca actcttatct ttgacggtct
    28201 cagggagcac ctgatacctc tgactggggt agctggattc gaaccaacgc atgagggagt
    28261 caaagtccct tgccttaccg cttggctata ccccaaaaat attttttaca gaaaactaat
    28321 tttctaatta gattagtttc cgaaatggaa agagagggat tcgaaccccc gaacccgaag
    28381 gagcggattt acagtccgcc gcgtttagcc tcttcgctat ctttccgtaa caacaaaaac
    28441 tattctatca tagtttttgt aagctggcaa gtgtttttgt ctaattttaa tggttcaaat
    28501 tgtagttttc aatgatattt tcaaccgctt gttcgagcgt tttgaagaag caatctactc
    28561 catcattttt taagacagca aatttctcat caagttctgc aatttctcca accacacgtt
    28621 taccaatttg taattgatag ccatcaatct tagtttcatt aaccataact ttgtgatcaa
    28681 tcagttggat ttcaattttt ttatcttttt tactcatcac tttacctcac tttgtctatt
    28741 ttacacaaaa tcaaaaaagc ttgcaagagc aagctctatt ttaatctact tttacaatcc
    28801 aaccttctgg tgcttcaata tccccaaatt gaataccgac caactcatcg tataaacggc
    28861 gtgtcacagg accaacttct gtttcactgt aaaagacatg taagttatcc ttgtactgga
    28921 taccaccaat tggagagata acagctgctg ttccacaagc tcctgcttcc acaaatttat
    28981 ccaattcatt aattggaaca tcaccctcaa ttactgtcat accaagtctt tcttcagcta
    29041 gttgtaagag tgaatacttc gtaatagatg gcaagataga tggactaagt ggcgtaacaa
    29101 attcattatt tgcagtaatg ccaaaaaagt ttgcagctcc gacttcttca attttagtat
    29161 gggttgctgg atctaagtaa atcacatcag aaaagcctgc tgatttagcg gccttacctg
    29221 gtagcaaact tcctgcatag tttcctccca ccttagcagc acctgttccg taaggagcgg
    29281 cgcggtcaaa cgcttcagaa acaataaaat tagtcggagc aagtccacct ttgaaatagt
    29341 ttcctactgg catggcaaaa atggtgaaaa tatattcttc ggctggttta accccaataa
    29401 tatctccaac tccaattaat aacggtctca ggtaaagcgt tgcacctgtt ccgtaaggtg
    29461 gtacaaagtc ttcattggct ttaaccactt gcttagctgc atcaataaat tgctctgttg
    29521 atacttgtgg catcaacaag cggtctgctg tggcttgtaa acgcactgca ttacggtctg
    29581 gtctaaatag ttgaatagag ccatctttgg tgcgataagc ctttaaacct tcaaaagctt
    29641 gttgcccata atgaagcgct ggagcgcttt ctgagatatg taaggtggca tcttctgtta
    29701 attgaccttt atcccattga ccatttttgt aataagaaat gtaacgaaat ggcaacttat
    29761 gatattcaaa tcctaggttg tcccaatcaa ttgctattgt catcatcgta cctcttttct
    29821 gcttttttct attgtaactt tttttcaata attttcaagt ttattctact cttttgcatt
    29881 aatattctaa atttaccata aaattaaaaa gagttttcac tctttttaac gtgttctagc
    29941 taccagtact tcttgatccg aaatagtgtc tgaaataaag gagccattgc tggttctttc
    30001 cgataacgaa agcgtttcta aagcaatctc gtaggtttga ccagtcttac taatgacttc
    30061 aagcatttgt tgattagttt cttcttcagg tatatcagtg aacaaatcaa actgctcagc
    30121 tgaagtatct gattgaacgc ctccggccag aaaaactcta tgaggtttgg tttttaactc
    30181 tcgtaggacc aatagtccac gattggcacg acttgtctga ggaatatcat cgacagccat
    30241 ccgtttgaga gaacccctct gagttaagac aaagaaagag ttacttgtca cagcaaaggc
    30301 tgaggcaagg ctgtcatcat ttttcaagtt aatgccttta acaccagcag atttcaaccc
    30361 ttgaataggc acttcctgac tggagaatcg caaagcataa ccatttttag tcactaatag
    30421 gaggtcttcc ataataactg gtgacaaggt aaccacgcgg tctttgtcat cctttagctt
    30481 cacatatttg gttgatttag atttataagt tcgccaagga cttagttctt tacgatcaaa
    30541 gcgtttgata aaaccatttt gtgtgacagc cacatacaag ccctggtcaa aggaagtcac
    30601 aatatcagcg tagagaatct cttcttctgt cgcgaagttt gaaatggttt gtgataaatg
    30661 ttcaccgata tccttccaac gtaaatcggt caattcatgg attggtcggt aaatgacatt
    30721 gcctaaagtt gtaaacaaca aaaggtgttc tgttgttttg gcctgtttaa cgaagatgag
    30781 ttcgtcatca tctctcttgc caacttcttc taaactagac gcattgaagg atcgtgggct
    30841 ggttcttttg aggtatccgc cccgtgtcac actaacaaaa gtttcttcct ctgcaatcaa
    30901 gctggcagtg tcaatctcaa tgatttgact ttccgcttgt aactctgata aacgaggatt
    30961 ggcgaatttc tttttcacct ctcttaattc acgtttcatg acgttataca tggttgcttc
    31021 gtcaccgata atagcggaaa gggtcgtgat taagtctctt aaatcatttt cttcattttg
    31081 gagagtaaca atatcggtat tagttaaacg ataaagctgc aaggttacaa tcgcttctgc
    31141 ttgttcctcg gagaaatcat agctgacttt cagattttct ttagcatcag ccttattgtc
    31201 agacgatcta atcaaagcaa taatctcatc taagatggac aagacacgaa tcaatccttc
    31261 aacaatgtgg aggcgttttc cagctttggc tttatcgaat ttagaccgct caataataat
    31321 atctttgcgg tggctgatat agcttgataa gattttttga agacctactt ggcgtggcgt
    31381 aaagtgatca atagcaacca tattgaaatt gtaattaact tgaagatctg tgtatttcaa
    31441 caaataattc aaaatggttt ggctatcagc ctcttttttc aactcaatag ctatgcgaag
    31501 acctgtgcga tcagattcat cgcgtacttc tacgatgcct gggactttat tgttaacacg
    31561 cacatcgtca atctttttga ccaagactgc tttgttaact tcataaggaa tctcagtgac
    31621 aataatttgt tgtttaccac ctttaagttc ctcgatttct gtcctagagc gaacaacgac
    31681 acgtccctta cctgtttcgt aggctttttt gatttcgtca gctccttgga taatgcctcc
    31741 tgtcgggaaa tctggcccag gcaaaaattc catcaatttt tctaaactag ctttgggatg
    31801 atcaatcata tacacaacag catcaatcac ttctgacaaa ttgtgcggtg gaatatctgt
    31861 cgcatagcct gcggaaatac cagaagaccc attgacaagt aagttaggaa aagctgctgg
    31921 taaaactgtc ggttcttttt ctgtgtcatc aaagttccaa gcaaagggaa ccgtattttt
    31981 ttcaatgtct tggagaagat aacccgcaat ttcagacaag cgagcttctg tataacgcat
    32041 agccgcaggc ggatcaccat ccattgaacc attatttccg tgcatttcca caagaatttc
    32101 acggtttttc caattttgac tcatccgtac catggcatca taaatagagg aatccccatg
    32161 cgggtggaaa ttccccatga tattaccaac tgatttggct gatttacggt aacctttttc
    32221 aaaagtattg ccatccttat tcatcgaata aagaatccga cgctgaacgg gtttcaatcc
    32281 atctcgaata tcaggaagtg cccgttcctg aataatatac ttggaataac ggccgaagcg
    32341 ttctcccata atatcctcaa gggacatgtt ttgaatgttt gacatattca aatagctttc
    32401 tttttcatta tttattaatt acctaaaaaa caatcgtctt tagcacttgt aattatctgt
    32461 gagaaacttg tatgactaaa acaccgtatt ttcttctaag gtaaacttga cgttatcctc
    32521 gatccactga cggcgtgggg ctgctttgtc acccatcaac acagaaacac gtcgctctgc
    32581 tcttgccaag tcgttaatag taacacgaat aagggttctg gtttcagggt ccatagtggt
    32641 ttcccataat tggttagcat tcatttcacc cagtccttta tagcgttgaa gaatagcccc
    32701 cttgccaaat tctctgcgca gatcttccaa ttcaccatca gtccaagcat aagcaatttt
    32761 ttcagttttg cctttacctt ttgacatttt gtaaagcggg ggaagcgcaa tataaacgtg
    32821 acctgtttct actaatggtc gcatataacg gtaaaagaag gtcaataaga gggtttgaat
    32881 gtgggcacca tcggtatcgg catcggtcat gataataatc ttatcatagt tgatatcctc
    32941 gagattaaag ttagctccca ctccagctcc aatggtataa accatagtgt taatttcttc
    33001 atttttcaag atgtcagcca ttttagcttt ttcggtattt aaaactttgc cacgtaatgg
    33061 taaaatagcc tgaaatttgc ggtctcgtcc ttgtttggct gaacctcccg cagaatctcc
    33121 ttcgaccaga tacaattcat ttttcttggc atttttggac tgggctgggg ttaattttcc
    33181 cgacaacaag cctttgtctt ttttgttttt cttaccatta cgtgagtcat cacgcgcttt
    33241 tcgagcagct tcgcgagcat ctctagcttt aatggctttg cgaactagat gagaagctac
    33301 ttctccattt tcgagtaaaa agaaggttaa tttttcagaa acgatactat cgacaatcgg
    33361 tctcgctaag ggacttccca atttgtcttt ggtctgtcct tcaaattgca agtgctgctc
    33421 aggaactaag atagataata ctgctgagag tccttctcga taatctgagc cctctagatt
    33481 tttatccttt tcttttaaga gattggtttt acgagcatag tcattcatcg ctttggtaat
    33541 agctgacttt agtcccgttt catggcttcc accatctttg gtgcgcacat tgtttacaaa
    33601 agataagata ttgtcggaga aaccatcgtt atactgcaaa gcaacctcga cttgaaagtc
    33661 ttggtcttga ccttccatgt aaataacagg tgtcaaggtt tccttatctt cattgaggta
    33721 ttcgacgaaa tcttgaaccc cattttcata atgaaactct tcaataatcg ggtcatctcc
    33781 acgaagatct gtcaagctca tcttgacatt tttcaaaaga aaagcagatt ctttcaatcg
    33841 ttctgaaatg gtattgaact taaagtcaat agttgaaaaa atcttatcat caggcataaa
    33901 tgttacaacc gttcctgatt tagacttagg agctgtgcca acttttttaa gggttgtaac
    33961 aggtttcccc ccattttcaa agcgctgacg atacactgac ccgtctcttg tgatttcgac
    34021 ttctaaccag gcagacaaag cattgacaac agaggagcca acaccgtgca aaccacctga
    34081 cgttttgtag ccaccctgac caaatttacc acctgcatga agaatcgtaa aaataacttg
    34141 aacggtggga atccccatgg catgctggcc tgttggcatt ccacgtccgc tatctgccac
    34201 actgaccgag ccatctttat tgataacaac cttaatatca tctccaaaac cagacaaggc
    34261 ctcatcgaca gcattatcaa caatttccca aatcaggtgg tgcaatcccg tagcatctgt
    34321 tgaaccgata tacatccctg gacgcttgcg aacagcatct aacccttcta ggacttggat
    34381 agcatcgtca ttataattat taattgtaat ttcgttctta gtcaacaccg acctccagac
    34441 agtttcatct tttttatctt acaagttttt taccaatttt gcaaaataat ttgctaattt
    34501 ttcccgaatt cttttccaga tctagttctc caatctatga atatgttata ataaggatta
    34561 tgaaattact actttttatt accattgcct atttacttgg ttctattcca actggactat
    34621 ggattggaca gtacttttac cacatcaact tacgagagca tggatcagga aatactggaa
    34681 ccacaaatac ttttcggatt ttaggtgtca aggcaggaac agctacctta gctattgata
    34741 tgtttaaagg gacactttca atattgttac caattatttt tggtatgact tcaatttcat
    34801 ccattgctat cggctttttc gcagttttag ggcatacttt tcctattttt gccaacttta
    34861 aaggtggtaa ggccgtagca acaagtgctg gtgtattgct aggctttgct ccgttatatc
    34921 tctttttttt agcatctatc tttgttttag ttctctattt atttagcatg atatctttag
    34981 ctagtgtggt ttcagctatc gttggtgtgt tatctgtttt aacatttcct gccattcatt
    35041 ttcttttacc aaattatgac tactttttaa cttttattgt gatattactt gcgttcatta
    35101 ttattataag acacaaagat aacattagtc gtatcaaaca tcatactgaa aatctaatac
    35161 cttgggggct aaatttaagc aaacaagtgc ctaaaaaata aatttactaa aagcctgact
    35221 ttctctgaaa gtcaagcttt tttgttttaa ctcatttgtt aattagggta aacaacctct
    35281 ccgtctgcaa tagtgtattt cacgacacct ttgagtttgt taccaataaa tggggaatta
    35341 ctagctttag aagcaaaatt ctcagtgatc agacgttctt gcttgtctgc aaaaataacc
    35401 aaatctgctg gaccattttc tgccaaataa cccgcatcaa aaccataaag caaagctgga
    35461 ttaagcgtca ttttttctaa tagtgacata agtgtaagat gacctggttc aaccaaatgc
    35521 gtcaaaccta atgacaaaga agtttcaagt cctgtcatac cagatggggc tttagtcata
    35581 tcatcaacag ctttttcatc cttgtgatga ggagcatgat cagtggcaat gactgtgata
    35641 acacccgatt tcaagccttc tatgacagct aagcgatctc tttgtgtacg aaggggagga
    35701 ttcatcttgg cgctggtacc tgcggtcaat aagagatctt cagttgttga aaaatgttgt
    35761 ggggacactt cagcagttac cttagcaccc aaccgctgag caaaagccac tacttcaacc
    35821 gattctgctt tagataaatg ttggatatga acatgagctt gcctgtcata agcaatcatg
    35881 acatctcttg caatcatact atattctgct acaccagttg ccccacaaaa atgaaaatgc
    35941 tcctcagcaa taccttcatt aaaaccaaga accccattta actgaggatc ttcctcatgc
    36001 aaagaaatga aagtttggtt ggcattagcc aaatcaaagg cttcttttaa aactttagaa
    36061 ctttctaagg gaataccatc atctgaaaag cttactgctc ctgcttccaa caaagcttta
    36121 aaatcagtca cgtcttttcc attaaaggct tgggtgacac tggcattggt gtaaatatga
    36181 atcttttctt tagctgcgct agctaacact tcctgtaaag tttcgacatc tgaaatgaca
    36241 ggattggtat tagccatcat gacaaccgta gtcacaccac ctgctgctgc tgccaaggct
    36301 cccgtgtgaa tatcttcttt gtgcgtttga ccgggttctc taaaatgcac atgaatatcg
    36361 accaaacccg gagcaacaat aagaccacta gcatcaatca cttgagcttc ttggcattca
    36421 atagctgaag ctatctttac aatctgttta ccatcaatca agacatctgc cacttgatct
    36481 ctctgcgatt tcgggtccat aacgcgtcca tttttgatga gtatcatgtt ttctccttta
    36541 tttatctgtt ttaaccataa gaccttacgt tctttaatag gaaaaatcta ctgcgctcat
    36601 ttcacttatt aaaggtatca aaatgtctag cttaagctat ttagttgaaa tggctggctt
    36661 attttagcca atctacgcca gtcatgccct ctttttctaa aatagcgttg gttcttgaaa
    36721 agggtttgct gccaaaaaag ccacgataag atgacaaagg gcttggatga gggctctcga
    36781 tgatgtggtg ctttggatta gtaatgaagg cttttttctt ccttgcataa gctccccaca
    36841 aaatgaaaac tacagggctg tctttctcat tcagcacttt aataacagcg tctgtaaata
    36901 gctcccatat taagcctgca tgcccattag cctggcctgc tggcactgtt aagcaagcat
    36961 tcaaaagcag aaccccttga ctagcccaag tgcttaaatc atgatggtca cgaggaccaa
    37021 tgtcatctgc taattctttt aaaatattaa taagggatgg cggagcagaa atctcttctg
    37081 gaactgaaaa tgataagccc tgggcctgtt tggggccatg ataagggtct tgtcctaaaa
    37141 tcaacacttt ggttttttcc aaaggagtga cttgtaaggc tttaaagaca ttttctcgtg
    37201 aaggatagac aagaccggaa gcataggctt catctaaaaa gtgattgatg cgaccgtagt
    37261 aatgctccgg caaaaatgat ttgattttct catgccaaat tgaatgagcc ataagattcc
    37321 tttctaatca cgtcagtttc taagttaaat acgatgtcct tttctatcta gtttaccaca
    37381 ttttaaatag aaagaccctg aaaatatgat atttccaggg ttaatattcg ttttttacag
    37441 gatattaaac aagtgctagc ttattttcta agaattcttg gtaacgttct aacaaaaaag
    37501 caataatcca atagataaag gctactaaaa tgtacatgga catgtagtcc cactccctac
    37561 caccgataat tttggcattt tgaaaaatat ctggtacggt aatcatggca gctagcgacg
    37621 aacttttaac catatcaata atgacattgc ctagaggagg aactgcaatt cgaaaggctt
    37681 gaggaagaat aattttctta accataagag catatggcaa ccctaaagcc ttagctgctt
    37741 cccattgtcc cttatcaata gcttctatag aagagcgaaa aacttcagaa atgtaggcag
    37801 cgctgaccat agaaaaacca agataagcgc aaagtaaagc tggcaattct aaaccataat
    37861 aaggcaaccc gaaataaaga acaaagagca ccacgatcat cggcacacct cgcataatag
    37921 agatgtacgc tctaacaaga taatgaatca acggctgacg tgaccttccc agaagggcta
    37981 ggcctaaacc taaaaacaga cccgtaaaaa aactgagcaa cgaaatacca agggtataag
    38041 gtaatcccga taacacaaat cctaaactat ctttcattaa tgggatatta atcatgtggt
    38101 taggactcct ttaacatcaa gtattagtta acgtctttcg tatcaatgac gggaattttt
    38161 ttgtctttgc caaaaggttt tgtcaaatct tgaccagcat agtaggtttc agatattttc
    38221 ttcaagctac catcttttat catgtcttta ataacagcat ctattttttt cttgaggcta
    38281 tcgtcttttt tgttcataac aattccttgt tcggttgggt tgtactgaac atcgctcatt
    38341 ttgacattta gatttggatt ttgagataac atgtaatcca caaataattt ttgagctggg
    38401 taatcatttg gaatgaaatc tgttcgaccg ttagccacat cgtttagata aacatctcct
    38461 gtaacattat cgtaggtcac cagttcagct ccctgttttt gtgctacttt catgtactct
    38521 gttccagaag cgccggctgc tttttttcct ttccagtcgc taagatcttt tttagccatg
    38581 tttgagctgc catcagcacg gacaatcatg cctcctaccc cgtatttata aggcgtagag
    38641 atattgtatt ttttctgacg ttttggggta atgtcaaagt tgttcaaaga aatatccact
    38701 cgaccactat caactgaggt aaaggcttgg tcaaatcctg tttcaacaaa cttgacttta
    38761 atgttaagtc gtttaccgat ttccttgacc atatctactt cgtaacctac taactcatta
    38821 ttatcgttgt aaaaagaagt tggctgatag gttcctgggg tagctacttt taacacccct
    38881 ttttctttga tagtctccca acttgatttt tgagatttat gtggcgcaca tgctgccaat
    38941 aaaaggctgg caagtccgat agacagtaaa gtaactgatt tcttcatagt aactccttta
    39001 ttaagataag aatagtataa cattttgatt aagttgtgtt atcagtgagc gctcaatctc
    39061 tttaaaaagt taatctataa tagttaaact gttttataag attattattg aggaatcttc
    39121 aataatcctg ctgcctcaaa ctgctctgca attcctaata ataggtcctc tctacctttg
    39181 gctgcaatta gttgaatgcc cattgggagc ccttccttag tttcgtaagt aggtaaacta
    39241 atagctggct ggccagttag attaggcaga gctgtgtaag gattaatagc cagactctta
    39301 ccaaacatgg tttcgaccaa attgaattgt tcttcactgc tgaaaatttc agcttgagcc
    39361 aagttagcca tgagtttcga atctgggaca agttctccgt gcttaggagc tggtgtgttg
    39421 gttgtgaacg tcaacaacag gtcataagtc tcatgaaaac tagccatggt tgcactgtac
    39481 gtatcccatt tttgaaggac ctgtgagtaa cgccaagctg ggatgtcttt acctgattgg
    39541 taaatagccc aggtcatggt ttccatatcg tctttggtca tcggacgtcc aaaagtgtct
    39601 tcaatatcag caaacatagc agccgtttcc acactattca taatataata atgacgaatc
    39661 acttctgtca tatttacagg aaactcttcc aattcaacta gctgatgccc ttgttctctt
    39721 agccaagtta ctgcttgtct taaagctttt gcagtatcta aagatactgg gctaccgtca
    39781 tttaaccgtt gataaaaagc aattgtcaag ggacgttgta gcgattgata aatactatct
    39841 tttgttaagg tggctagggg gaaggggctt tccatttgtt ccatctgtaa atagtagagt
    39901 agatttctag tatctctaac ggatttggtc aaggcaaaat gaactgaggc tccttgccag
    39961 cttcgataag aaccaggtcc gacaggcatg cgcccgcggc taggtttgag cccaattaag
    40021 ccattaaacg aggctggaat ccgaatagag ccacccccat cactagctgg tgctagtgca
    40081 ctaattccag atgacactag agcagcagct cccccagatg agccaccagc atttcgtgta
    40141 ttatcctttg gtaaattcac tggttcgtgc aaactgctat cgctaatatt tttaaagcca
    40201 aattcaggtg tgttagaacg ccctaaaata ataaatccca agtcttctaa ccttttcacg
    40261 aataaatccg ttttagtcgc ttgatactct ttaaacaatc tggaacctga ggttgatgag
    40321 tgacctttta attcttgtcc caaatctttc aaaaaaatgg gaactcctgc aaaaggctta
    40381 cctgaaaaat ccctttgttt ggcttcttct agggctgctt caaagcgttc tgaggtaata
    40441 gcatttaagg tagggttcaa ctttttagcc ttataaatag cctgggttac taattctagt
    40501 ggtgtggtct gaccggtctg aacagcaata gccatagccg tcgcatcttg ataagtcata
    40561 gctactcttc ctttctaaga tatgaatcct aaaacattct tttttattaa aacacaaata
    40621 aaagaaaacc accattaaaa ggttagaaat tagaatgtga tgtgtaagaa atgtagacaa
    40681 aacaatcaaa acataacatt ttgaattggg gttaaagaga ttttttcatt aaaaataaac
    40741 ctggctgata gttccttatt gttgccagtt tacctggtct tctttaaatt ttttaagtaa
    40801 atgtaaacca tcattagtaa tatagccttg caactttgca actgcaatta gctcagtata
    40861 attagaaagc gtaatcaact tgataccagc ttctttaaaa ttctgactag cttttggtaa
    40921 ctcataagtg aaaatcgcaa caactcccag aacgtcagct ccctcacgac tggctgctgc
    40981 ggctgcattt agtaccgaac cacctgttga aatcaaatct tcaatgataa ccattttttg
    41041 gcctttaagc acacgcccct caatctggtt gcctgctcca tgatcttttg gcttactgcg
    41101 aatataagca aaaggaagag tcattttatc tgctataatg gcaccgtgag gaatacctgc
    41161 tgtagctgta ccagcgatga cctcaacttc tgggaaatga gccctgatag tttccacaaa
    41221 cccattctca attaaatcac gagtcttagg ataagaaagg gtgacacgat tatcagtgta
    41281 aataggtgat ttaatgccag atgcccacgt aaaaggatct tcaggcttta aataaacagc
    41341 tttaatatca agtaattgag ttgcaatttg gcttgctagt gtcatttctt attctccttt
    41401 ttcttcttta acagacaagt gttggtttcc taatcttatc tgttagcctg cccactcagc
    41461 ttttatggct tggtaagcag ccactgggtc ttttgcctgg gtaattggtc gccctaccac
    41521 aatataatca cttccaatgc gtcttgcttg gtttggagtc atcactcgtt tttgatcgcc
    41581 aatattactg cctttaggac gaatgccagg agttaagcaa gtaaatcccg taggtgtcac
    41641 cgccttgata gcttctactt cttgtgccga acaaaccacc ccatctagct gagctttagc
    41701 tgcccccttg ctgtaatgca aaacagactc taaaagactc gtttgaatat tttgatcacc
    41761 acgcatttgg tcttcactgg ttgaagttag ctgcgttacc gcaattaagg tagggccttg
    41821 acctagtcct tctcgagctg cttttagcat ctctacacca ccagctgcat gaacagttgc
    41881 catatcaata tctaattctt taagaactgc catagcagca cgaacagtat ttggaatatc
    41941 atgtaatttt aaatccaaaa atacattatg ccctaacgat ttgatatacc gaacaatatc
    42001 tggtccttgc gcataatata attccatacc aattttcacg tatagctttt cttctgctgg
    42061 aaataagtct aaaaatgctt tagtctcctc aaatgatgaa aagtctaaag caataattgg
    42121 gcgttcttct ttcataactt atcctttcta taatgtgtca atatctcatt aagcaccaaa
    42181 aagcctcgct accaaagaag cgaggctaca gcaaactaaa agggaattcc ctttttttat
    42241 cttgtatacc ttgtttgcct ctctggactc acttaaaggt gtctaaattt aaactattat
    42301 agctttttta aaacaagatg tcaaaacttt ttgttatttg aacacatctt tcctactata
    42361 tctttttttg tatacttacg tcaactcatt ttatcatttt tacccaatca tttattcaaa
    42421 ctcacctgtg ggtaccttag ggctttggta atattttata aaagcttctt cttcaagatt
    42481 aaccctctgg atatgttcat agagcacatc cggaatagga gcttccttaa gtgacaaagc
    42541 actcactttt aaagatgaaa aaacatcact taagacccca aaaactcgaa tgcgtttatc
    42601 tccaataata atttgaaaga cttctttgga ctggcataaa tctatcagat gatccactcc
    42661 tttaatccaa ttatcgacaa tatccaaagt tttgttcatc aacttaaaag ctgattcttc
    42721 ataccaaaag gtagactcaa tcaagcgttg ctgcatatcc aaggtatcaa tggccgcaac
    42781 aatatctccc aactcagact gtactaaatg agctgaaaat tcccctccaa gtatagaagg
    42841 agttcgttct acaagcttca gttcatctcg aaaaagagtc aaatagtgga tactgaccaa
    42901 taatttttgg attaagattt gatgactatt ttgttcagta acattcacac gaccaccacc
    42961 ctttgattag aagaatacct atttgttaat actattaata taacacaaga aaaataaaag
    43021 ggcaatgaaa ttgatagatt caactctcaa aattgaatca tatttatcct aggccatatt
    43081 gagaaaagac tccagcgctt tttcaaagtt ctaaacccat gatgacgttt gtgcttgcgt
    43141 ctttattttt tttgagctag actgtactta tgagaattca acaactgcat tatattatca
    43201 aaattgttga gtgcggctct atgaatgaag ctgctaagca attatttatc acacaaccaa
    43261 gtctatccaa tgcggtcaag gatttggaaa tggagatggg cattaccatt ttcaaccgaa
    43321 atcctaaagg gatcaccttg acaaaagatg gggtggaatt tttatcttat gctcgccaaa
    43381 ttatagagca aacatcactg ttagaagacc gctataaaaa ccacaatact ggccgagagc
    43441 tctttagcgt ttcatctcaa cattatgcct tcgtagtcaa tgcatttgtc tcactcctca
    43501 agcggaccga catgactcgg tatgaactct ttttgcgcga gacaagaact tgggaaatca
    43561 ttgatgatgt caaaaacttt cgatctgaaa ttggtgttct ctttatcaac gactataacc
    43621 gagatgtctt gaccaaatta tttgatgaca atcatttgac tgcctctcca ttatttaagg
    43681 cacagcctca tatttttgtc agcaaatcaa atcctctagc aactaaaagc ctattaagca
    43741 tggacgactt gagagatttt ccttacctga gctatgacca agggattcac aactcctttt
    43801 atttttcaga agagatgatg tcccagatgc cccacaacaa atcaattgtg gtcagtgacc
    43861 gtgccaccct ctttaactta atgattggtt tagatggcta caccgttgcc agtggtattc
    43921 taaatagcaa tctaaatggt gaccagattg tggctatccc gctagatgtc cctgacgaaa
    43981 ttgacattgt ttttatcaag catgaaaaag ccaacttgtc caaaatgggg gaacgtttta
    44041 tcgaatacct tctcgaagaa gtaacctttg attaatgtct ttttcaatcg tcatactaaa
    44101 aaaacacggt agtaagacct gtatcggtca agttatcgtg ttttttgata aatatcagac
    44161 aatagattgt ctccaaaggc tcaactcctt tttaataatg gcattcatgt catcaaagta
    44221 aaaaggctca cgtcggtaga ttacaccaga aaaagtcgcc aaggtgataa tgttttccaa
    44281 aaaagcctga gaagaaaaat gagtggtgcg atgcagttcc tcttcatcaa aaggcttaat
    44341 caaatgagcc agaggattac ggacagcctt ttcaaaatgt cgtaacaaaa aaaccaagtc
    44401 cttgatttcc tgaggtaagg aggtcaacgt caacatgtct gctaagcttt tggtcgtcac
    44461 attgcgagac tgcttttgag acaggtaagc cttgaaaacc tcgtgatcag attccaacat
    44521 ggcctgaaaa tgccaggtat catattggtc attctttgta tcaaaaatat aagccttgaa
    44581 gtttggaatt tctgacgtga ttagtctgag aaaaagtcgg taaagaatag gcgacactga
    44641 cctgacgaaa tcaataatag caccgttttt tactttaact tctaaatcca aaatgtaatt
    44701 ggcaatctgc tcatcttcaa gcgattggtt aagccataat gaacagtggt actggtcttc
    44761 aatggtccga ttctctgctg catgttccgc cagaaaggca aggttcaatt ctctgcgctc
    44821 ttttaacatc tctagtaaat agagggcagt tttagggata tttggcaagg ctttagcaat
    44881 tctaaaggcg tgattataag catagacatc caaaaggatg tctagtgttt gattattagt
    44941 cattggcaat taaggtttcc aagccgacct tcatcatatc agtgaaagtg gtttggcgct
    45001 cttcagctgt agtatcttcg gttggatcat tcaagttatc tgagatcgtc atgattccaa
    45061 gggctttgac atggtgctga gcagctaaat agtaaagagc tgctgcttcc atttcgatgg
    45121 ctttaacacc caatttacca agagccatat tacgctcagg catgttagtg taaaagacat
    45181 cagaagaaag cacattccca acgtgagttg ttactcccat ttctctggcg atatggtaag
    45241 ccttgtcaag taaaccaaaa tcagcaattt gtgggaaatc aaattctgga aagtcattac
    45301 ggataatatt tgagttggtt gctgctgctt gggcaagcac caattcacgc acatgaactt
    45361 ctgggtcaat cgcacccgca gtaccaacac ggatcaatgt tttgacaccg taatcaacga
    45421 ttaattcacg agcatagata gagatcgaag gcattcccat accagttccc atgacagaaa
    45481 cgcggtgccc tttgtaagtt cctgtgtagc caaacatgtt acgcacttcg ttaaaacaca
    45541 ctgcatcttc taggaaattt tcagcgataa atttcgcgcg caaagggtct ccaggtagaa
    45601 gaattttatc agcaatatcg ccttttttgg cagaaatatg gatagacata aattatgata
    45661 ctgagggcat taaaagaacg atggcaaaat aggaagttgg cagaaaatcc tgattttcta
    45721 gacaacttat ctttttgacc aagtccttag cccgaattca aattaatgaa ctcagccctc
    45781 acctttctta tttttttatt ttttataagg aagaaaaaca gttctccgcc cgtattcaat
    45841 tcaatgaata cagttagccc tacctttcct tttattttgt agaaggtcag agggcggaca
    45901 gaaagcgact gaaatttagg gaactaacgc catgtgtgag ctcaaaagtt agtttatcta
    45961 gtttccgagc tttccgcccg tattcaattg aatacaaccc cattcctttc ataattgata
    46021 ttgaatagaa gagagctaaa aatcgagttc aaataagaaa ggactaactc tctttccttt
    46081 attatttaaa aattaatacg aagaaatgtt cgagtccata ctcaatttaa accgaatacg
    46141 tctctttccc ttcgttggtt atgttagtta gaaaagctag aatctgagtt caacattcat
    46201 ggaactcaca ctccgtgatt attttttgat ttttttattg cgaacagatg tgccaaactg
    46261 acgacataaa ataacattcc tgtaataatg ctccagttga ttttgttcgt cctacaactc
    46321 cgataaaata gctttaacaa gacctttgaa atcttctttg atatgttggg tcacttccac
    46381 aacttcttca tggttcaatt ctgattgaaa accagctgca aagtttgtaa tagctgaaat
    46441 accaagtact ttaagtccag aatgagcagc cacaataact tctggtacag ttgacatgcc
    46501 aacagcatct gctcctaata ctttgaaagc acggatttct gctggtgttt cataagttgg
    46561 accagttaag cccatataaa caccatcttc tagtttgatg ttcatttttt cagccacttc
    46621 atgtgcctta ttgcgataaa ccttagtata ggcatcagac atatcaggga agcgtggacc
    46681 aaactcgtcc aagttttcac cgattagtgg gttatttccg gtcatattga tatggtctgt
    46741 gattgccatc aaggtacctg ggccgtagcc gataccacca gctgcatttg tgacaagaac
    46801 gccttcacat cccagagctt tcatgacacg aacagggaaa gtcaccactt caagcgggtt
    46861 tccttcgtaa aaatggaaac gaccttgtag ggcaagtact ttacgaccag ctaaatcacc
    46921 gtaaactaac tttccagcat ggccaacaac tgttgattta ccccagtttg gaatatctgc
    46981 ataatcaata acaatagcat tttctacttc ttcagcaagt tcaccaaggc ctgaacccag
    47041 aattaatcca aattcgggag tttcaattcc tttagtaact aaaaagtctt ttgtttcgtt
    47101 aattttagtc atcaatgaca tctttttctc cttttacctc aatagggttc acactctcgc
    47161 catcctcttt catataagga ctaggcaggg gaacaggaag aatatccagt aaggtctcag
    47221 aaggacggca aagttttact cctttgcgag tcacaacaat aggacgattg atcaaaattg
    47281 gatccgtcaa catggcatta ataacgtctt tctcagctac tgcttgattt gctaatccat
    47341 gagcctcaaa ttcaggaaca ttggttcgca agagttctcg cgcagagatt cccatcgatt
    47401 gtaagagctc aattaaagtc tcccgattag gtggcgtctg caagtactca ataatagtag
    47461 gctcaatacc tgcatggcga atcatggcaa gaacattacg tgaagtgcca cagtttggat
    47521 tatgataaat cgtgatttct tccattatca attactaact ttcttatttt aagtgaccaa
    47581 ggaatgactc cccaatcatg gctgtatcaa ctccaaaatt ttctgcaaca gtcgctgaaa
    47641 tatctgcaaa atgcccttgt ggaataagac catttccagt aaaggatgca gagtaggcaa
    47701 gcaatggaat gtattcacga gtgtggtcgg tgcctgcata ggttgggtca ttaccatggt
    47761 cagcagtgat gagaagcaaa tcgtcttctt tcatatttgc gatgatttca ggtaggcgat
    47821 tgtcaaattc atgcaagcag tcgcgataac cctctgggtc acgacgatga ccaaagttgg
    47881 catcaaaatc aacgaggtta gtaaaggaga atcccttagt aaactcaggt aattgaagag
    47941 tcttaataag cgtgtcaata ccatggctgt tcgacttatt gtgtcccatg tcgtttgtaa
    48001 tacctgaacc attaaagatg tcattaatct taccaacggc ataagttggg acacctgcgt
    48061 cagccagttt gttcaaaacg gtatcttgga aaggagacac tgcatagtca tgacggttag
    48121 ccgtacgtgt aaagtttcct ggttcgccta cataaggacg cgcaataata cggcctaaaa
    48181 gggcagggcg ttcaagcgtg atggagcgag catattcaca aatcttgtaa agctcttcca
    48241 caggaataat gtcttcatgg gctgcaattt gaagcactgg gtctgctgag gtgtaaacaa
    48301 tcaactcacc tgtttccatt tgacgcggac caaaatcatc aataaccgca gttcctgaat
    48361 aaggtttgtt ggcctcgcga atgattttgc ggcctgagaa ttcttcaatc ttagttaaaa
    48421 tctcttctgg gaaaccattc cagaaagtgt caaaaggttc cgtaatgttc aagcccataa
    48481 tttcccaatg gcctgtcatg gtatcttttc caagagatac ttcttccaat ttggtcacgt
    48541 aaccagtagg attgtcttct gttggaacag tttttagtgg tattggacga gaaatgtttc
    48601 caagtccaat ttttgccata tttggaaccg ataagcctgc tgcttctgaa atatgaccga
    48661 gagtgtcaga atctgtatca gctacaccag cattaaagaa tttgtcagca tctggtgctg
    48721 caccaatacc gacagagtct aaaacaacca agtgaatacg attaaatttt gacataagtc
    48781 tccaatctag gggaggttac ggttgactcc ccaataccta taaaatatat taacgctgtt
    48841 atatgctaag actagcctgc tgtaggcgct tctaaaacag taacgccgtc tttactagca
    48901 acaatcacct tatcaaccat accattaaag agaccgtgtt ccacaacacc aactgttcca
    48961 tctagcaggt gaccaaaagc aacaggatct ttaatacaac ccaaatcaag atcaataata
    49021 tagttttgca tgtctgtcac caaacgacta tcacctttca tacgaaaaga cggtttgtaa
    49081 ccagcttttt caaagacacg gaacaaacga tcagcaccat attgaacgac ttcgactggt
    49141 aatttaaagg cacccagatg ctctaccatt ttagaagcat caacaaccca gatgtattct
    49201 tttgttggtg ttgcaacaat tttttccatt agtaaggctg cgccaccacc cttgattcca
    49261 ttgaaatcct tatccacttc atcagcacca tctacggtga ggtcaatgct atcaatatca
    49321 tctatcgatt ttaaaggaat gcctaagcct tctgcttgct tgctagtcac acttgacgtg
    49381 gtcacaccaa ccacttgcag tccttcttgc tttacgcgac gaccaatttc ctcaacaaaa
    49441 tagtaagccg ttgacccagt tccaagccca atggtcatgc catcagtcac atattgcgca
    49501 gcggtaacac cggcaatttt tttgagtgct tccatgattc ccccttataa tagaattgcg
    49561 tcttagcact ttattatacc atgaaagcgc caacttgtta atgaaaacat ttgtttttct
    49621 ctcattgaca gtctaagtgc tatagaatag gatgatcttt ttaaaactta tgatttattc
    49681 taaagtcatc atataagcac ttacttttcg tgtttattta gctttttgaa aatcaagtgt
    49741 ttttgtttga cctttactga ccttttgatt ataataatac tataagatga gtaaaaataa
    49801 tgtctgaatc attttattag caaagggttt cctatcatgt gaaatcctaa caacgattct
    49861 gcgactgctt acaaacatct tatagtctat tttttattgc gaggtaactg atatgacaca
    49921 tttttctggt aaagatcctt ttgtaaatat ggatgatatt ttcaaccaat taatggcaaa
    49981 catgggtggt tatcgtagcg aaaatccccg ctatctcgtt aacggtcgcg aaataactcc
    50041 cgaagaattc caacactatc gtcaaacagg acaactccct gtagcgacaa ccaaagcaac
    50101 aaattctcaa atgttaacac ctaaagctga tagtgtattg actcaactcg gaactaactt
    50161 aacacaagaa gctcgtcaag ggcatcttga ccccgtcatt ggtcgtaaca aagaaattca
    50221 agataccgct gaaatcttgg cacgacgaac taaaaataat cctgtccttg tcggagatgc
    50281 tggtgtcggt aaaactgcag tcattgaagg tttagctcaa gccattgtta atggagatgt
    50341 cccagctgct attaaaaaca aggaaatcgt atcaattgat atttctagtc ttgaagctgg
    50401 tacacagtat cgtggttcct ttgaagagac cattcaaaac ttgattcaag aagtaaaaga
    50461 agctgggaat atcatcttat tttttgatga aattcatcaa atagttggtg ctggtgccac
    50521 aagcagtgat tcaggctcaa agggacttgc agatattctt aaaccagcct tgtcacgagg
    50581 agaattaaca ctgattggtg ctactactca agacgaatac cgcaatacta ttttaaaaaa
    50641 tgctgctctt gctcgtcgct ttaatgaagt taaagtgaat gcaccatcag ctgaagatac
    50701 ttttcacatt ttaatgggta ttcgtaatct ttatgaacaa caccaccata ttacccttcc
    50761 tgataatgtt ctaaaagctg ctgttgatta ttctattcaa tacatcccac agcgtagcct
    50821 gccagataaa gcaattgact tactggatat gacagctgct catttagctg cgcaacaccc
    50881 ggtcactgac ttaaaaacac tcgaaactga aatagctaaa caaaaagaga gtcaagaaaa
    50941 agctgtcgcc aaagaagact ttgaaaaagc tttagcagca aaaacacgga ttgaaacatt
    51001 acaaaagcaa attgagcaac ataaccaaag ccaaaatgtt actgctacag tcaatgatat
    51061 cgcagagtct gtagagcgct taactggtat tcccgtctct aacatgggga ctaatgattt
    51121 agaaaggctc aaaggaatta gcagccgctt aaaaagccat gtcatcggac aagatgaggc
    51181 tgttgcagca gtagcacgtg ctattcgacg aaatcgagca ggttttgatg atggtaaccg
    51241 tccaataggt tctttccttt ttgtgggtcc aactggtgtt ggtaaaacag agttagctaa
    51301 acaattagca cttgatttat ttggttccaa agatgctatt attcgacttg atatgtccga
    51361 atataacgat cgcactgctg tgtcaaaatt gattgggaca acggctggtt atgttggcta
    51421 cgatgataat aataataccc taactgagcg tgttcgtcgt aacccttatg ctattgtctt
    51481 actagatgaa attgaaaaag ctgatcctca aatcattaca cttcttctac aagtgttaga
    51541 tgatggacga ttgacagatg gacaaggaaa tactattaac tttaaaaata ccgttattat
    51601 tgctacatca aatgccggtt ttggccaaca agatacggaa acttctgggt ctaacattat
    51661 ggatcgcatt gcaccttatt tcagaccaga gtttttgaac cgttttaata gtatcattaa
    51721 atttaatcat ctgcaaaaag aaaatttaga agaaattgtt gatttaatgc tagctgaagt
    51781 aaatcaaacc accgcaaaaa aaggtattag tttaacgatt gctgatgacg ctaaagctca
    51841 tttgatagat ttagggtaca atcacgctat gggagctcgt ccattacgac gtattatcga
    51901 acaggaaatt cgtgaccgta taactgatta ttatcttgac catccagaag tcaaaaaact
    51961 tcaggcaatc ctaaaagaag gacaactagt gattaagcaa aaagatcagt aataaacttc
    52021 ttcaattgaa taattttcaa aacatgaaaa aatccgtctt gtgactacgt tatacacttg
    52081 acggactttt ttttagcgct tttttaaaaa taattttttc atcgcctaat aactcaagtt
    52141 tctttccaaa tctggtgtaa tcaaaataac cattaggtgt gagcaatcct tttgaaaaag
    52201 aagtatgtcc cgctgctact tcttctttga tatcaatggt gtgcaaaata cgattaactt
    52261 ttcctttgag aacaacagca gataaatgtc ctgttttttg attatatttg cctccgatct
    52321 gagaagctaa tttctcaaaa gcttgatcta aaatcatttc taattcatgg ctagctggca
    52381 tactattctc aataatttgg cgttctttac tactcaagtc accaacaact aaatgatttt
    52441 cctttaatgt ctttaataat tcattttggt gataagcagc taaacgtttg acaaaaatgt
    52501 ctcgttcaac ttgaacacta aaactcaagt caacgttgtt atttttaact gttagtatca
    52561 tctgagatga cgttaataaa ctatcatcaa taagagggct ctgatgcaaa ccagtaagac
    52621 gtttagcaaa atcatcgcct aacttctgat caatgcttgt tgttcgccag ataccttcaa
    52681 tagagctatt acgatatcga acaccaaaaa gtacagcaag caagataaga aaaataataa
    52741 atacaaaaag ggcattgcat tgatggcata acaatgactt tcctttagag cgcccttggg
    52801 ctacttgtgt catgactcat ctccttaata taaaatacta gcatgtcatc attaaggcgt
    52861 caagtaaaat cggtaaacct aactatgatt ttttatgttt tcgataagaa aaacaccctc
    52921 tactgagaat gttttagcat agataaatca acacctatcg ctagacctgc tctaaaatag
    52981 catcccaaga atcatcgata ccaatacgtt ctaccgaaga aaatactata aaagtatcag
    53041 atttgtcaaa attcaaagct ttcttgacca cagactcatg tttgttccat ttgccacgag
    53101 gaatcttatc tgctttggtt gccacaacaa taacaggaat gtcatagtac ttcaaaaagt
    53161 catacatctg aatatcttct tttgaagggg catgacgtaa atcaaccaaa ctcacgacag
    53221 cacgcagatt atctctggtg gttaagtatt cttcgatcat ctttccccat ttggcccgtt
    53281 cacttttaga aaccttggca tagccataac cagggacatc cacaaatcta agcttgtcat
    53341 caatattgaa aaaatttaat agttgggttt ttcctggctt gcttgaggtt cttgccaaat
    53401 ttttgcgccc caatatggta ttaataaaac ttgatttacc aacatttgaa cgtccagcga
    53461 gggcaatttc tggcaaatca tcttgggggt aatgagattt attggcagca ctcaataaaa
    53521 ttgaggcgtt atgggtattt aagacttgtt cttcagccat tatttctcct taagctgtct
    53581 ctaaaacggg ttttgacttg ccttcgacag cagccttggt gatacgcact tttgtgacat
    53641 cttcttggct tggaatttca aacataatgt ctaacatcgt ttcttcaata atagaacgaa
    53701 gaccacgtgc tcctgtctta cgttcaatgg ctttattggc aatggcttct aaggcttcct
    53761 tatcaaatgc caattcgacg ccatcgtaag aaagaagggc ctgatattgc ttgaccaggg
    53821 catttcttgg ttctgtcaaa attttaatca aatctgatgt gtttagttgt tcaagggctg
    53881 ccacaactgg taagcggcca ataaattctg gaataagccc aaacttttga atatcttctg
    53941 aaataatttc ttgcatataa gaagcattgt catcaatttt acggctgttt tgaccaaaac
    54001 caataacttt ttcacctaaa cgttgtttaa caatctcctc gatgccatca aaagccccgc
    54061 caacaataaa caagatattt tttgtatcaa tttggatcat ttcttgatta gggtgcttcc
    54121 gcccaccttg tgggggaaca cttgctaccg taccttcaat aattttaaga agggcttgct
    54181 gtacaccttc accagataca tcacgtgtga tagatacgtt ctcacctttt ttagcaatct
    54241 tatctatttc atcaacataa ataataccac gctctgctcg ttcgacatta tagtcagcag
    54301 cctgaatcag tttgagtaaa atattttcca catcttctcc aacataccct gcttcagtca
    54361 aagaagttgc atctgcaata gcaaatggta cattgaggct tttagccaga gtttgtgcta
    54421 agaaggtttt tcctgaacct gttggtccaa tcatcaaaat gttggacttt tgtaaatcca
    54481 catcatcgtc atcacgactt tctgtaaacg aaacacgctt ataatgattg tacacagcaa
    54541 ctgataaagc acgtttagca cgatcttgac caaccacata ttgatttaaa acatcaagga
    54601 gctcttttgg ttttggaact tctgtcaaat cggcaaggac ttcttcagcc aattcttctt
    54661 ttataatttc ttgtgataaa gctacgcatt cattgcagat gaaaacatta tttcctgcga
    54721 taattttttt aacgtcatcc tgacttttac cacaaaatga acagtatact ttaatatcgt
    54781 tagttctact tcctgccatt tcttataatt ctcttacttt ctcttatctt ttactgaatt
    54841 ttaatattag tcaatgtgtt gtattggttt ttggtgattc ttgcttgata gctaaatctt
    54901 caccagtgct gtctttgcaa gtcacatctc aaaacaatgt accatagaag gcgtgaatac
    54961 tattaaccag attagtaaac gcatttaata agaacagact tcctaagcca taacgattct
    55021 ttttaaaagc tgggatggca caaagaacac aaatcacgca caaaaaagcc gtaaatactc
    55081 caaaaatact acgttgcatc gcttatcatc tcctcagact atttgtgttt taaagcagta
    55141 acggtaaaat cataaagatt tttagcgtct cgagcataga acgtttggct ttcttgcgtg
    55201 aaatgactaa aatctagatt tggtcggtag gtatctcctt taaaacgatg atggataact
    55261 gtttttatga tacgatcgaa ataaccatta aacgcttcta aaaccttatt cccaccaatg
    55321 atataaagtg ttttatcctg cgcatggtac cactccaaga ctttttcaat actagtcatt
    55381 gtcaacacct catctacttg ataatctcta tttcttgtca tgacaagggt ttgacgttga
    55441 ggaagtcttt tgcaattcat tccttcaaaa gtcatacggc ccattaagat tgcttgatgt
    55501 agcgttgtct ttttaaaatg ttctaactct tttggtaaat accatggtaa cttacccgca
    55561 acaccaataa gccctgcttc atcctctgcc caaatagcaa taatttcctt tgtcattaat
    55621 tatttccttc attttaaact ctatttattt taccaaattt atgaacaaaa tgctcacaaa
    55681 aaatctaaat aaagtttaaa tagcaaggtc aaagtgcaac tgaggcttca ctggatcata
    55741 attttgtaat tcaaaatcat ctggtttaat atcaaagaaa ttagtcccat ctggaacatt
    55801 taaaacaagc ttaggctgac tggcaactgg ctgtcgtttt agtaattctt gagcctgatc
    55861 aaattggttg tcataaatat gtaaattgtt aacaaaataa aagaacttac ctattttcca
    55921 tccaaaatgt ttagcaatca tcatctgcaa ggcaacgtat tgcatggcat tgatgtgatg
    55981 agcaactaat atgtcattag aacgttgcgt caaactagca tctaaataaa gatcttcccc
    56041 tacacgtcta acatcaaaca tgatttgaaa ggcacatggc aacaaccctt ttgtctcctc
    56101 aaatgcttca tagtcccaga gagaaatgac attacgacga ttccaaggat tttctgctaa
    56161 ttgctttagt atttttgaga tgatatcatg ttttttgact acagcaccat aacgttgccc
    56221 aatagtacgt gtctggtcaa cttcccattc attccaataa tgaacattat atttagcttc
    56281 tagcacatct aaactgttag actgatcttg atatatccaa aataattcct ttatagcaga
    56341 tttaattgga atgggtcgta aagttgtgat gggaaactct cctttagcta aatcatattc
    56401 agcaaacgcc ccagtaatat acttagaatg agctgttcga ccatctttat atttaggacg
    56461 tgcttgctca ctcaatgatc cttcatttat aattttttga atattagctt taaaaatctg
    56521 gtcagctttt gtcatctcta ataacctttc tgtaagcata cttcatacta ttctaactaa
    56581 ttttaccaaa aaataccaga ataaataaac attcgaaaaa cttcatgtgg tcatgaacat
    56641 tttaactatt aaaggataaa acaaataact ttatcagtct aattttgcag taaaatgtga
    56701 tagaatgggt aagactataa aaataactaa aagaaggaca aatatgacaa ttggaattga
    56761 taagattggt tttgcaacca gtcaatatgt cttgaaattg gaagatttag cgcttgcacg
    56821 ccaagtggat ccagcaaaat ttagtcaagg gctactcatt gaatctttta gtgtggcacc
    56881 aatcactgaa gacattatta ctttagctgc ttctgcagca gatcaaatct taaccgacga
    56941 agatcgagct aagattgata tggttatttt ggcaactgaa tcgagtactg atcagtcaaa
    57001 ggcatcagct atctatgtac atcacttagt tggtatccag ccttttgcac gttcctttga
    57061 agtaaaacaa gcctgctata gcgcaactgc tgctctagac tatgctaaac tgcatgtggc
    57121 ttctaagcca gattctcgtg tccttgttat tgctagtgat attgctagat acggtgtagg
    57181 atctcctggc gaatcaactc aaggatctgg tagtattgct cttttggtaa ctgctgaccc
    57241 tcgtattctt gctctaaatg aagataatgt ggctcaaact agggatatta tggacttttg
    57301 gaggcctaac tatagtttca caccttatgt tgatggtatt tactctacca agcaatatct
    57361 caattgctta gaaaccacgt ggcaagctta tcagaaaaga gaaaaccttc agttatctga
    57421 ttttgctgca gtttgtttcc atattccatt tcccaagtta gccctaaaag gtctaaataa
    57481 cattatgaat aacacagtac ctcctgaaca cagggaaaaa ctaatagaag cctttcaagc
    57541 ttctattact tatagcaaac aaattggaaa tatttatact ggctcacttt atctaggatt
    57601 gttatcttta cttgaaaata gtaaagtatt acaatctgga gataaaattg gtttttttag
    57661 ctatggttcc ggtgccgtaa gtgaatttta ttccggtcag ttagttgctg ggtacgataa
    57721 aatgttaatg actaatcgac aagctttact agatcaacga acacgtcttt ccgtttctaa
    57781 atacgaagac cttttctacg aacaagtcca attagatgat aatggtaatg ccaattttga
    57841 catttactta actggaaaat ttgctctaac agccatcaag gagcatcaaa ggatctatca
    57901 taccaatgac aaaaactaat cttaactgga gcggtttttc aaagaaaaca ttcgaagaac
    57961 gcctccaact tatcgaaaaa tttaaactac ttaatgctga aaacttaaat caactcaaaa
    58021 cagacgttct tttgcctatc caaacagcta atcaaatgac tgaaaatgtc ttaggacgat
    58081 tggctttgcc ctttagcata gctcctgatt ttcttgtcaa cggttcaact tatcagatgc
    58141 cttttgtcac ggaagaacct tctgttgttg ctgcagcatc tttcgcagca aaactaatca
    58201 aacgctcagg tggttttaaa gctcaaaccc taaaccgaca aatgattggt caaattgttc
    58261 tttacgatat cgaccaaata gataacgcta aagccgccat ccttcataaa acaaaaaagc
    58321 taattgcatt ggcaaataaa gcttatcctt ccattgttaa aagaggtgga ggcgctagaa
    58381 ccattcattt ggaagaaaaa ggagaatttt tgattttcta tctgactgtt gatacccaag
    58441 aagctatggg agcaaatatg gtcaatacta tgatggaagc tcttgttcct gatttaacaa
    58501 gactgtctaa ggggcattgt ctaatggcga ttttatctaa ttacgcaaca gagtcgcttg
    58561 ttactactag ttgtgagatt cccgtgcgcc ttttagatca cgataaaaca aaatccctac
    58621 agttagctca aaaaatagag ctagccagcc gactagctca agtagatcct taccgggcta
    58681 ctactcataa taaaggtatt tttaatggca ttgatgcagt ggtaatagcc acaggaaatg
    58741 actggcgtgc tattgaagca ggggcccatg cttatgcctc aagaaatggt agctatcaag
    58801 gacttagtca gtggcatttt gaccaagata aacaagttct gcttggccaa atgaccctcc
    58861 ctatgcctat tgctagtaag gggggatcta tcgggcttaa ccctactgtt tctatcgcac
    58921 atgatcttct taatcaacct gatgccaaaa cattagccca attgattgca tctgtggggt
    58981 tagctcaaaa ctttgctgca ctaaaagctc tgacctcatc tggcatccaa gctggtcaca
    59041 tgaaactaca tgcgaaatca ttagctcttt tggcgggggc aacccaagac gaaattgctc
    59101 ctttagttaa tgctttacta gctgataaac caataaatct agaaaaagca catttttact
    59161 tatctcagct aagacagtct tagatttaaa caactaatat aaggcaatca cccatgtaaa
    59221 ccaattgttt acatgggtgt ttaatctttg tttctataca tacaagtctt agaaatagtc
    59281 tccataacat actctattta ctcatctact ctattttgag tttgatgagg tatttataaa
    59341 tattttatat tatgttctaa aaaagctcta tcatctttgt agtaggtgac actattgtag
    59401 aggtgatgaa gcctaattga cctgctgcaa gcgtccatat agtaagtagt cgactccctt
    59461 taattcttta atagttttac agtcaagagc acacataatg atttttaatt cttctttcca
    59521 gccgttaacg atagcaatca cacgctcggt tgggtatttt tcgaccaatt ctaaaacggt
    59581 gcgtgagagt cccactgcac gtgctcctag gacgaaacat ttaatcatgt ccaaggggtg
    59641 tctgacacca cccgaagcta agatttccac ttggtccatc agtccttgtg catttagcaa
    59701 gcactgaacg gtggtttgtc cccaatcgtt taagtaagag cggtctcctc ctctttgatt
    59761 ttcaatatag gcaaatgagg tccctcctct tcctgaaata tcaaaggttt gaatgcctag
    59821 gtcatgtgct agcttgatac tattgacatc catgccaaaa ccaacttctt tgagaatgac
    59881 aggaactggt atttgactag cgtattcagc aaggtgtttt ttccaggtat gaaagacgcg
    59941 ctcaccctct ggcatcagca actcttgcat cacattcaca tgaacctgta aaaagagggg
    60001 ctgcatttct tgaaccgttt gttgtcctag cgccacaggt ttatctagac caatattcgt
    60061 ggctagtttc aagttatctg ccacctcatg taaacgatag gaatcgtcgt taggattttt
    60121 taaagcagcg ctataagacc ctgtcaccat gacaatccct gttgctgctg ctactttggc
    60181 caatttttca ttgacagctt tgcctttttg acttcctcct gtcatggcat tgatgtaaaa
    60241 gggaaagtcg aagtcttgcc cggcaaaatg agtactgaga tcaatatcag acaaatcata
    60301 gctaggtaag gaatgatgta tgagttctat gtcatcaaaa gcattataag gcgattggta
    60361 cttgagggca tatttgatgt gatcatcttt acggttggtc attctctccc cacctttctt
    60421 gataaagtaa tgcgatatca gcttcttgcc atttggaaat aagggtatct ctagcatcct
    60481 gattaaaggc aagcgcgatg ccacaatctc caccaccaga acctgaagat ttagcaacag
    60541 catcttgctt ctgacaagca gctaccaagg ttactagctt aggatggtag atagctggat
    60601 gaagctcttt taggagatga cttgctcctg ctaaactttt cttgagttct tccttatgac
    60661 cttcttgcaa agctaccata gctgattgcg ttagctggta acttgttctt aagaaagctg
    60721 gggtaatgct cgctgtcact tgttgaatca tctgccttga aatagcaggt attttagtcc
    60781 agccaaccaa aaagtcacaa ggcagggctg gttgaatgac ttggatatgg taaccccaat
    60841 ctttgacgag aagttttttt aagggcatgg tctgcagcca gttactgacc tgttctcggt
    60901 caaaggaggt ataggcgact aaagtctggt aagcgataca ggcaatatcc cccatagacc
    60961 cattatctcc ttgcttaagc aaggtataag ctgctagttt aaagagtaac tctggggtta
    61021 aagtgatctg ataataggcc gataaggcct ttaaggttaa gagggtgacg ctaccacttg
    61081 aaccaatgcc aaattttttg ccatcgcgct ccatttttcc tgttatgatt agggaaaatg
    61141 gctccagttg gtcaattgac tgtcctagat aatcagcaaa ggttttaacc gttgcttgaa
    61201 tcagtgcata agaggcatcg ggtgtcatgc ccgctttatg ggaaaacata tctgaagcta
    61261 attgaatatg ggctgctggg ctaatttctg ctgtcatcat cagtggaata aagtgaatta
    61321 aggctttttg tcctggtatt aagatagcat attcgcctgt gaggtatagt ttcccacctg
    61381 tttgcacaca ataattagac atctggtaaa tcctttgttt ttgaaacgat aatacggtag
    61441 tttttgccaa gtcgttctgc tagttgagcc aagtcttttt ctaaacatag gaccttcaca
    61501 tttggacctg cgtccatggt aaaataacaa gcaaaacctt cttgacgcag ttctttcaca
    61561 gcctccatag cctggtaaga ctcttttgtt aggtaagaaa agggaggatt ggctgtcttt
    61621 gtcgtggcat gcatggccaa ggcattagct tctgttaact gaccaacttt ctcaaaatta
    61681 ttagttttga gataggtaag catatgttgg taatcgattg ccgattgttc tacccattca
    61741 tcaaatgtag ttgaggtatc gcggcataac ttcatgccct ctcggctaga aattggcttt
    61801 ttcgcagcat ttaagaccag cataatcatg gccattttca agtcagtctc aaccttgtaa
    61861 atagcaccac tatctttgtc ccaagcagca actgggccaa aaaaagaacg agaagatgat
    61921 cctgaggcga acttggcctt ttgtgctaaa gctttctgat ctagctgagt gtcaaataat
    61981 tgatcacagg ctttaaccaa ggctgataag ccactggaac ttgatgataa acctgcagct
    62041 gttggcatat tattttgagt ttccatcttt acaaaagcct gaccaggttg gcgaaattgg
    62101 tcaatgatag tagaaatttt ggtatgttct tcgtcatttt gcaagacacc gttaatgtaa
    62161 aactgatcac tggttgcagt atctggtaag aaggaaacgc tggttgtggt gaacatgttt
    62221 tccaaagtca aagaaatgct agaggtcgaa ggaatcatct tagcttgatt ctcttttccc
    62281 caatatttga taatagcaat gttagcatag gatgttacgg ttataacatt tggatccacg
    62341 tgtttacggc tccttcttct tttaatctgt gactgatttt ttcggccatg tctttggtat
    62401 ctgctagggc aatcatacag ccacccaagc caccgcctgt catcttagcg cctaaagcac
    62461 ctgctcttag ggcagcctcc acaagttggt cagctttact gatgctaacg ccaatggcct
    62521 ttaaagcaga gtgcgcttgt gtcatgagct gaccgatagc caccttattt ttttggttaa
    62581 tcgctctttc aagggcttgt gtcaaagctc ctagtttagc cagatagggc aaattcgttt
    62641 cctcaaactg tgctacctta ttgacggcct cacgtgtatg accgtgaatc cctgtatctg
    62701 caatgataag ataaccattt aaggcgattt cgatagtttc aaagccaatg tttcggataa
    62761 acttaatggc atggtcacta aggcatgtct tggcatctaa accactagga ttggtgtggg
    62821 caataatttc tgccttgttg actaaaattt ccaacaaatc atccgagagg ggttcttgac
    62881 aataagaaaa aacggctcta atagctgcaa tagaaacggc tgccgaagat cccataccac
    62941 gcttttgtgg cacttgtgaa acaatctcat aagcaattgg ttcttgtagt cgctgcaaat
    63001 aatccaaggc agcataaatg gctgttgata gagtatcata aaaatcaaag accaatggtt
    63061 tatcagctgg aaaaatatga caaaccacct caatatctgt taaaggcaaa gcaatagctg
    63121 ggtagccata cacaacagca tgctcaccta tcaaaatgat cttactgtgt gccttaccat
    63181 atccaatgtt ttcgttcatt ttttatcttt tcattactaa ttcttctaaa cttttacctt
    63241 actattttac actaaatgat tagaaaatgc gatgatatat ctttttaaaa tcatttcttc
//
LOCUS       DATURV010000005        53312 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DATURV010000005 DATURV010000000
VERSION     DATURV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53312
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.005"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(400..1806)
                     /gene="lacG"
                     /locus_tag="VCF75_000284"
     CDS             complement(400..1806)
                     /gene="lacG"
                     /locus_tag="VCF75_000284"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEP2545879.1"
                     /translation="MTKTLSKDFIFGGATAAYQVEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGTNVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(1894..3591)
                     /locus_tag="VCF75_000285"
     CDS             complement(1894..3591)
                     /locus_tag="VCF75_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP2545880.1"
                     /translation="MNGLIAQIEKGKPFFEKMSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIESLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFMSTMLAAIVGFLLMAANPAKEGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFIWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLVVVDVIIYYPFLKIYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKAQFEN"
     gene            complement(3591..3908)
                     /locus_tag="VCF75_000286"
     CDS             complement(3591..3908)
                     /locus_tag="VCF75_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP2545881.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(3932..4915)
                     /gene="lacD"
                     /locus_tag="VCF75_000287"
     CDS             complement(3932..4915)
                     /gene="lacD"
                     /locus_tag="VCF75_000287"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP2545882.1"
                     /translation="MTITLTENKRKSMEKLSVDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECSAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSKERFGVDVLKVEVPVNMKFVEG
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLEFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(4919..5848)
                     /locus_tag="VCF75_000288"
     CDS             complement(4919..5848)
                     /locus_tag="VCF75_000288"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEP2545883.1"
                     /translation="MILTVTLNPAIDVSYPLDELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNEFGETVKATGCIGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPMLSVDEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIGIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTRDFDALKEVLQDKLFDGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKIKEV"
     gene            complement(5896..6411)
                     /gene="lacB"
                     /locus_tag="VCF75_000289"
     CDS             complement(5896..6411)
                     /gene="lacB"
                     /locus_tag="VCF75_000289"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP2545884.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHEIIDCGTYDNVRTHYPI
                     FGKKVGEAVASGKAELGVVICGTGVGITNAVNKVPGIRAALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(6446..6874)
                     /gene="lacA"
                     /locus_tag="VCF75_000290"
     CDS             complement(6446..6874)
                     /gene="lacA"
                     /locus_tag="VCF75_000290"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP2545885.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKHEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            7319..8092
                     /locus_tag="VCF75_000291"
     CDS             7319..8092
                     /locus_tag="VCF75_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP2545886.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLETLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
     gene            8742..9029
                     /locus_tag="VCF75_000292"
     CDS             8742..9029
                     /locus_tag="VCF75_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HEP2545887.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            9019..9354
                     /locus_tag="VCF75_000293"
     CDS             9019..9354
                     /locus_tag="VCF75_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP2545888.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQHKMEQIISD
                     IEKLEVFPEVGFDADEKYGSKISHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     RSDYIKLFK"
     gene            9506..9976
                     /locus_tag="VCF75_000294"
     CDS             9506..9976
                     /locus_tag="VCF75_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP2545889.1"
                     /translation="MEEFTKPTHKTYSEIFEKWYQAYHDTVEPTTASRTLDLFRLHIL
                     PVMGELPINKTSPLDCQKFITDKAKTFKNIKQIKSYTEKVFDFAIKMKLLKHNPMAEI
                     IMPKRKKIRIENYWTVQELQEFLAIVLQKEPYKHYALFRLLAYSGLKERRAMRS"
     gene            9939..10487
                     /locus_tag="VCF75_000295"
     CDS             9939..10487
                     /locus_tag="VCF75_000295"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP2545890.1"
                     /translation="MAALRKGELCALKWVDIDFQSETLSVNKSLGRLDGKTIEKSTKN
                     DFSVRKIKLDSETISILQEWKAVSQKEKTQLAVAPLSIKSDFIFTYCTREGSIEPLHA
                     DYINNILSKVIKQHSLKKISPHGFRHTHATLMIEIGVDPVNTAKRLGHASSQMTLDTY
                     SHATAVGEDKSIKQFANYLKNN"
     gene            complement(10619..11011)
                     /gene="rpsI"
                     /locus_tag="VCF75_000296"
     CDS             complement(10619..11011)
                     /gene="rpsI"
                     /locus_tag="VCF75_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEP2545891.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(11032..11478)
                     /gene="rplM"
                     /locus_tag="VCF75_000297"
     CDS             complement(11032..11478)
                     /gene="rplM"
                     /locus_tag="VCF75_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEP2545892.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(11696..11902)
                     /locus_tag="VCF75_000298"
     CDS             complement(11696..11902)
                     /locus_tag="VCF75_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2545893.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSR"
     gene            complement(11899..12405)
                     /locus_tag="VCF75_000299"
     CDS             complement(11899..12405)
                     /locus_tag="VCF75_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545894.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAFVLTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPKYDERQKQILLKGYT
                     IGFWFMIVVIWLSLFISRFTEGLVSASFLFTLALWGGLAVQTTYCNLSMGASIFYRHY
                     LDAKEADE"
     gene            complement(12550..13410)
                     /locus_tag="VCF75_000300"
     CDS             complement(12550..13410)
                     /locus_tag="VCF75_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP2545895.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHAKNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(13507..14025)
                     /locus_tag="VCF75_000301"
     CDS             complement(13507..14025)
                     /locus_tag="VCF75_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEP2545896.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            complement(14029..14775)
                     /gene="rlmB"
                     /locus_tag="VCF75_000302"
     CDS             complement(14029..14775)
                     /gene="rlmB"
                     /locus_tag="VCF75_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEP2545897.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKSRGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(14822..15619)
                     /locus_tag="VCF75_000303"
     CDS             complement(14822..15619)
                     /locus_tag="VCF75_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545898.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQTNQSANQEIDSTSNPFRSATNHRSTSLKRSTKNEKLTSTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLLGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(15815..16228)
                     /locus_tag="VCF75_000304"
     CDS             complement(15815..16228)
                     /locus_tag="VCF75_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEP2545899.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(16221..17564)
                     /locus_tag="VCF75_000305"
     CDS             complement(16221..17564)
                     /locus_tag="VCF75_000305"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEP2545900.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEAAGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKLAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFVNYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKALHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFATADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            complement(17592..17822)
                     /locus_tag="VCF75_000306"
     CDS             complement(17592..17822)
                     /locus_tag="VCF75_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545901.1"
                     /translation="MNKIRVGLYVIMSLSILVFSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(18300..18881)
                     /gene="cysE"
                     /locus_tag="VCF75_000307"
     CDS             complement(18300..18881)
                     /gene="cysE"
                     /locus_tag="VCF75_000307"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEP2545902.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTIRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(18890..19642)
                     /locus_tag="VCF75_000308"
     CDS             complement(18890..19642)
                     /locus_tag="VCF75_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEP2545903.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSVALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQESAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(19635..21767)
                     /gene="pnp"
                     /locus_tag="VCF75_000309"
     CDS             complement(19635..21767)
                     /gene="pnp"
                     /locus_tag="VCF75_000309"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEP2545904.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIIAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASIKALIPRPPKPEKKEEKHD"
     gene            complement(22047..22778)
                     /locus_tag="VCF75_000310"
     CDS             complement(22047..22778)
                     /locus_tag="VCF75_000310"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEP2545905.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPLIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEGVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDAKH"
     gene            complement(22788..24167)
                     /locus_tag="VCF75_000311"
     CDS             complement(22788..24167)
                     /locus_tag="VCF75_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEP2545906.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLTFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            complement(24194..24478)
                     /locus_tag="VCF75_000312"
     CDS             complement(24194..24478)
                     /locus_tag="VCF75_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2545907.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKDADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(24471..26531)
                     /locus_tag="VCF75_000313"
     CDS             complement(24471..26531)
                     /locus_tag="VCF75_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP2545908.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVISGSWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKKQIKQDYESLFYLVKRSLSPLEKQLGKLVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSEWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGARQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(26883..27152)
                     /gene="rpsO"
                     /locus_tag="VCF75_000314"
     CDS             complement(26883..27152)
                     /gene="rpsO"
                     /locus_tag="VCF75_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEP2545909.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(27328..27528)
                     /locus_tag="VCF75_000315"
     CDS             complement(27328..27528)
                     /locus_tag="VCF75_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545910.1"
                     /translation="MTMLGYRLSAEVLGSAILPLLAGYLIRQAIAQVYSDLKTAFWLF
                     GLASSVAYGGLVKAKRAHSMTR"
     gene            27654..27911
                     /locus_tag="VCF75_000316"
     CDS             27654..27911
                     /locus_tag="VCF75_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP2545911.1"
                     /translation="MTQNVCQQNITYTVKERFASYILKAQANQEVHLNFTLLANRFGI
                     SDRHLKHVLKQLIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            28037..28651
                     /locus_tag="VCF75_000317"
     CDS             28037..28651
                     /locus_tag="VCF75_000317"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP2545912.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(28684..29226)
                     /locus_tag="VCF75_000318"
     CDS             complement(28684..29226)
                     /locus_tag="VCF75_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP2545913.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
     gene            complement(29357..29785)
                     /locus_tag="VCF75_000319"
     CDS             complement(29357..29785)
                     /locus_tag="VCF75_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP2545914.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            complement(29895..34292)
                     /locus_tag="VCF75_000320"
     CDS             complement(29895..34292)
                     /locus_tag="VCF75_000320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEP2545915.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLTRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRKNHLPNLVKQLEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKAAITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHDRKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLARYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRAKKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVQNTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVVPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEIGILGNMPEDNQLSLFDDFF"
     gene            complement(34546..36402)
                     /locus_tag="VCF75_000321"
     CDS             complement(34546..36402)
                     /locus_tag="VCF75_000321"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEP2545916.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRNAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSIASMDDIPKEVLEEIKAELAAWMISGEDTIAYSTKSSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRTVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            complement(36600..37859)
                     /gene="rseP"
                     /locus_tag="VCF75_000322"
     CDS             complement(36600..37859)
                     /gene="rseP"
                     /locus_tag="VCF75_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEP2545917.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHT
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            complement(37932..38726)
                     /locus_tag="VCF75_000323"
     CDS             complement(37932..38726)
                     /locus_tag="VCF75_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEP2545918.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            complement(38739..39488)
                     /locus_tag="VCF75_000324"
     CDS             complement(38739..39488)
                     /locus_tag="VCF75_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEP2545919.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            complement(39704..40069)
                     /gene="yajC"
                     /locus_tag="VCF75_000325"
     CDS             complement(39704..40069)
                     /gene="yajC"
                     /locus_tag="VCF75_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEP2545920.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            complement(40185..40559)
                     /locus_tag="VCF75_000326"
     CDS             complement(40185..40559)
                     /locus_tag="VCF75_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEP2545921.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKDVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            complement(40674..44171)
                     /locus_tag="VCF75_000327"
     CDS             complement(40674..44171)
                     /locus_tag="VCF75_000327"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEP2545922.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSDDGFQSSSKEAKPVLDSSSANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDKDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADL
                     TKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLGFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKVS
                     DDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWAKATDSRAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDSSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            complement(44369..45982)
                     /locus_tag="VCF75_000328"
     CDS             complement(44369..45982)
                     /locus_tag="VCF75_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEP2545923.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTNGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEDLRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDLEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAKELDVPALKAIFSKWQTKLKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFAKDLAGAQ
                     VIITNTDVDTVLETKHLQPWDAFCLKLKA"
     gene            complement(46112..47245)
                     /gene="ugpC"
                     /locus_tag="VCF75_000329"
     CDS             complement(46112..47245)
                     /gene="ugpC"
                     /locus_tag="VCF75_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEP2545924.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            complement(47543..48391)
                     /locus_tag="VCF75_000330"
     CDS             complement(47543..48391)
                     /locus_tag="VCF75_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEP2545925.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQALPADLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLYLKQLDDLAFAH
                     LFLLKY"
     gene            48732..50054
                     /gene="ska"
                     /locus_tag="VCF75_000331"
     CDS             48732..50054
                     /gene="ska"
                     /locus_tag="VCF75_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEP2545926.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSQPAQGGKTEQDLNPKSKPLATDKGAMSHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGGLDFTPLLRNQYHLTTLAVGD
                     SLSSQELAAIAQFILSKKYPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYEINPKTGIKEKMNNTDLISEKYYVLKKGEKPYDPFDRSHLKLFTINYVDVNTNELL
                     KSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHDDTNRIIT
                     VYIGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK"
     gene            complement(50152..50592)
                     /locus_tag="VCF75_000332"
     CDS             complement(50152..50592)
                     /locus_tag="VCF75_000332"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEP2545927.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLVGVGPDDNAEDLAYA
                     VRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLAS
                     QLYDSFNEQLAEFVPVERGVFGADMQVSLVNDGPVTIILDTKCH"
     gene            complement(50607..52826)
                     /locus_tag="VCF75_000333"
     CDS             complement(50607..52826)
                     /locus_tag="VCF75_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEP2545928.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKADVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(53078..53188)
                     /locus_tag="VCF75_000334"
                     /pseudo
     CDS             complement(53078..53188)
                     /locus_tag="VCF75_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129544913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT        15808 a        11631 c         9845 g        16028 t
ORIGIN      
        1 aaaagtgcaa aaatgacttt tttcatattt tttagtaaga tttttgagat gtcacataag
       61 aagtagccct ctgatataaa aaacaaaact cgttctttac agctatttta caatctaatc
      121 gcttctcgct ataacatcaa tctctaatca gaaaatggtt actttactta tttaatcagg
      181 tcaggagttt gaatgacaga agtcaaatat ctgaaaaagg atgttaaata aaactttcaa
      241 aacccatttt caccaactaa aaacacttat aacccgatct tacaactaat ggattaccat
      301 caacagcaaa taatactatg cttggaaaat gtcaacatca tctacttttt gtgactacga
      361 gcatgacgtg taaaaatgta ttaaaaaagt atctcaaaat cattcaatca cttgagtttc
      421 tgctactttt ttataccagt aggcactctt cttaggataa cgttcttgag tttcaaaatc
      481 aacatagaag agaccgtaac gtttttcata gccatttgac catgaaaaga catccatcag
      541 tgaccacata aagtatcctt taacatttgt accatcagaa atagcatcag aaataacttc
      601 taagtgtttt ttcacataat cgatacgtcc atcatcataa acagtattat ctacaaactc
      661 atctttgtaa ccaagaccat tttcagtgat ataaattttc ttgtagttag gataatcggc
      721 tttgacacgc atgatttgat catataagcc ttgtgggaag ataatccaat cccagtccgt
      781 ttttggaaca tcaacgggag cttttcttcg acccacaccc ttgatttggt atttagagct
      841 gcctttttcc cctttaccat tatgaatgat ttcagtctca ccatcaaaag cttgcatcca
      901 atcactcata taatagttaa taccaaggaa gtcattcaaa tcttttgcag cgtctagtgc
      961 agcaaaatct tcctcacgaa gatcaagttc accaccattt acctcaagga tatggttaac
     1021 accttccatt gttttatctg aatacttacc tagataagtt gcatcaagaa taaatttatt
     1081 atggatgata tcttccagtt cagctgctct aacatcatca ggattgttag cgtcaaatgg
     1141 atacttagtt ggaagagcat ggacaacgcc gatttcacct gaataaccac tatctttaaa
     1201 gagtttgact gcacgagcat gagagaccat catgttatgg tgtgattgga aaactttagc
     1261 aagatcatat tggatacctg gagggaattt accaaccaag tattggccat caccaatagg
     1321 cccaatttcg ttaaatgttg tccaatagtt aacttctgag aattctttaa aacaaaattc
     1381 tgcataattt acaaaatgtt caatgttctc acgattgagg aaatcaccat ccgagtggag
     1441 agcttctggt gtatcaaaat ggtgaagtgt aacaaaaggc tcaacatgac gcttatgaca
     1501 ctctgcaaag agattgtggt agtattcaac tcctttaggg ttaacttctc cttttcctgt
     1561 tggaaaaata cgagaccagg caatagagat acggatgccg ttgacaccaa attcttcact
     1621 aagtttcaaa tcgactgggt aacgattata aaaatcactt gctggctcag ctgtgtacca
     1681 atagttgtct tctaagtatt tatcccaagc tactggtcct ttgccatctg tatgggtagc
     1741 accttcaacc tggtaagcag ctgtagcacc accaaaaata aaatctttag ataatgtttt
     1801 tgtcatctca gtttctcctt gaatatcttt caaaaataaa gttcccaaaa caatttttgt
     1861 gtttgagaac tttacgacat ttaaactgtt ttcttaattt tcaaactgtg cttttacaaa
     1921 atcaagagct ccttgaccat cacgggtcaa tttgatgtat tgagcccctt ctgttttagc
     1981 taatttaata tcaagtttat cagtttccac tttcatatct tcatagttag aagctacttg
     2041 aggtgcaagg ataacaagtt gatattgtgg taaaatctca cggtgagcac cataacttcc
     2101 tgctgctgca gtgacaggtg caccatattc tttagctgct ttattcaaag cgtttgcaag
     2161 caaaccactt gtaccaccac cggcacaaag aacgagaaca ttagtttgat cagtgatttc
     2221 tttggtttct ttagctgctg atttttcaag aatagcatca gcttttttag tatcaaagtt
     2281 tgctgctact ttttctttga gggcatctga tgaattcgta cctgctgctt cttcagcaag
     2341 aatttgctca tcataaattt tcaagaatgg gtagtagata atcacatcaa caacaactaa
     2401 caaagctgcc aaaatgaatg ctagcacttg gaagtttgta ccaaggataa gacccaatgg
     2461 agctggtgtt gtccaaggta agtttgcagt gaaactgttc attccaagtg tgtcaatgaa
     2521 gaatttgaaa atccatacat tgacaattgg tgccaagata aatggaataa agaagattgg
     2581 gttcaaaaca agtggggcac caaacaaaat tggttcgtta acaccaaaga atgttggaac
     2641 aaccgatgca cgtccaatcg ctttgttacg ttttgatttt gttaaccaaa tgaacatgaa
     2701 tggaacgacg agagttgcac cagtaccacc catggtaacg atgaacattt gagtccctga
     2761 agtcaaaatc ttgtcagcat gttcaccagc ttgaagtaat tgaaggttgg tttcagcatt
     2821 tgcgtaagta atcgctgcaa tggctggttc aacaatagat ggtccatgaa taccaacaaa
     2881 ccagaagaaa gcaaatgcac cgaagataat agtgattcca acatagccat ctgctgcttg
     2941 gaacaatggt gcaagaagtt taccaattga ttctgcaacg ttaacgccta aagtagtatg
     3001 aacaatcaat tcaatcacat aaagcagtac aactgataac gtaaatggaa tcaaatcctt
     3061 aaatacttga gagatatttg gtggaacctc ttctggcata cggattgtaa cattgttttt
     3121 cacacaggtt ttatagacat ttacggtaat aaaagctgcg atgaaggctg tcaacaaacc
     3181 ttttgttccc ataaatccag ttaagaaacc accttctttt gctggattgg ctgccataag
     3241 taaaaaacct acaatagcag cgagcatggt agacatgaag ttgatttggt ttgtgcttgg
     3301 taatgatcga ttcattgagt cagttaaagc tttggctgtc gtaccagcca caaacaaacc
     3361 taaaataccc attgagtagt tgtatggcgt catcaagaga ctctcaatat ccttactcca
     3421 atggaagccc catgcatttg gaacataggc aatcaagata aagatacttg aaaacaagat
     3481 aactggcata ccagcgataa aaccatcacg aatagcgcga agatagatgt ttcgagacat
     3541 tttctcgaag aaaggtttcc ctttctcaat ctgagcaatt aatccattca ttattttgct
     3601 cctctcttat aaagttcaat catgtgtttc attaaatctt gtaataaaat ggtcgtcatt
     3661 aaatgatctt gaccatgcat taaagtgaca ctataagcta agtcatctcc agctgcttct
     3721 ttttgtagta gacttgtctg agcattgtga gcatcattca agcagtcact acctgcagcg
     3781 attaattctt ctgctttagc ataatctcct tcttgcgctg ctttaagcgc ttccaaaaat
     3841 tttgaacgag catcaccagc ataggctacg atttcaaaac ctaaaagagt agtttcttct
     3901 ctattcattg tagtttcctc cgaaaataat gttacatttt ttcagtccaa ggacttgcag
     3961 ttttatctag tactttgttt aattcgtcaa tatttttgaa accttctgta cgcaaccatt
     4021 cacgagctgc ttgtggtcct tcttcgatat agacttttac agatcctgcc caggtagcac
     4081 gtccacataa gacaccgttg aatttagcac ctgattctgc tgcaaattcc aaagtatctt
     4141 ggaacaactt ggctgaaaca ccagcactca aataaatgta tggtaaatca gtagaagctt
     4201 cttgatcacg aaaagcttga gctgcttctt cttttgtaaa gagaacctca ccatctgcaa
     4261 agccttctac aaatttcata tttactggaa cttcaacttt caacacgtca acaccaaagc
     4321 gctcttttga gaaaactttc attgcttcgt taactttatg agctttaact tttgcaaact
     4381 cagggcttgc attgtcagca attttctcat cataagttag aatttctaag tagaatggaa
     4441 tatcttctgc actgcattca gaaccaatac gttcaatgta agcttttttc tgctcgttaa
     4501 catcttgatc tccatcaata tcatagtaaa gaaggaattt aactgcttct gctccagctt
     4561 ctttaatacg ttttgcagac caaacatcta agcaatcagg taagcgacta gttgttgttg
     4621 catcgtatcc tgttttctca tatgccaaca acaagcctgc ttcttctgaa cgaacacgac
     4681 ttgctggcaa gccatattca ggatcaagta gaattgagga tgcaaatggt gttagttctt
     4741 cagaaactaa acttttaagc tcttcaattt gttctactgt tggctctttt gtttgatgtt
     4801 gagccatcat acgtttgaga gctccacgtt ggtcaaaagc taaggcagaa ataacaccat
     4861 ctacacttag tttttccatg cttttacgtt tgttttcagt taaagtaatt gtcatttttt
     4921 atacctcttt gattttaata gattgatata acttatcgta atttgccatg ttaacatgcc
     4981 cagttgtttt ttcttgcgca tttaacatcc ctaagacatt tgcttttgtt aataaagctc
     5041 gatcatcttc atcattcgct aaacctgaag caatacctgc cactgtagaa tcccctgaac
     5101 ctacagcgct tacaatttta attttaggaa tgtcaacgtt ataaaacgta tccttgtgct
     5161 ttgcaaatac accatcagca ccgagagaaa cgattatcca ttcaattcca tcaaataact
     5221 tatcttgtaa gacttctttt aaggcatcaa aatctcttgt catttcttta cctagaagtt
     5281 gagaaagttc ttcaagatta ggcttgataa ctgttggttt actatcacct tttagtactg
     5341 cttctaaagc atttccagaa caatctagaa cagtcttctt accattaaga ttggcaatac
     5401 cgattaactt ctgatagtaa tcatctggca taccagcagg cagactgcct gagagcgtca
     5461 ctacgtcaac atcattcagt aaatacttaa aatgatgagt aaatccatca atttcatcta
     5521 cacttaacat cggtcctttt tctaaaatct ccgtttgatt accttcgtgt aaaatggcta
     5581 tacaagttct agtatcacct gaaattttat agaagcgact taaaatcgaa tcaggaagat
     5641 gattaatgat aaaatcaccg ctttctccac ctatacaacc agtagctttg actgtttcac
     5701 cgaattcatt aagaactcgg gaaacattca agcctttacc accgggtgtc tttgtaacgt
     5761 ctacaactcg attgacagta tcgcatttta gctcatccaa agggtaggag acgtcaatag
     5821 ctggatttaa agtaactgtt aaaatcacaa tagctccttt ttctatatat atccctatag
     5881 taatttattc aaatattagt cgtgatattc accacggtcc catttttcta ggaattcatc
     5941 aaagaagtgt gggttgtttt gatcttggct agcatgttgc aagctatcca ttttttcaat
     6001 ccatttttta ttttcttcag ttggtttgta ttccgcggct aagaaagcat cgataatatc
     6061 attcatcaat aaaccaccga tgatttttcc accaaaacca ataacgtttg cgtttagctc
     6121 ttcttttgag tagatagcag atgtcatatc tctaacgagt gctgcacgaa taccgggaac
     6181 tttgtttaca gcatttgtga taccaacacc agtaccacag ataacaacac ctaattctgc
     6241 tttaccactt gctactgctt caccaacttt tttaccaaag attggatagt gtgtgcggac
     6301 attatcgtat gtcccgcaat caataatttc atgaccttgt gattttaggt aatccacaac
     6361 agcgattttt tcataagtaa caatatggtc acaacctaca gcaattttca tttttgttct
     6421 ccttgtttta acctatttca atcaattagc acattttatt aagcatgtcg acacgaactt
     6481 gatgacgtcc accatcatat ttaccttcga caaagccttt gataatgttc tttgcaattc
     6541 ctggagcact aatttcagaa ccaagcgtga tgattcgagc attgttgtgt cctcttgtca
     6601 tgtatgctga gcgctcatct gatacttcag ctgccaccat tcctttgatt ttagtagcta
     6661 ccatgaaaga accaacacca tatgcatcaa tgacaatacc taagttatct tcggatgcat
     6721 taacttcttt tgcaacagct aaagttgtat ctacaaagtc acgtacttcg ttttcactaa
     6781 catcaacgac ttcgtgttta ccttctttaa ggtaatcttt gataacttct ttaagttctt
     6841 gacctgcttt gtcagcaccg ataataattg ccattttatt tctcctatga tttagaataa
     6901 gtaaatgtgg aaaacctacg tagatagtta attaacgatt tgcaacatct tttgaaataa
     6961 cgctttcatt atttctatga cctaatcata cacccaccaa acataaaagt caacattttt
     7021 tgtttattct tacaaaaaat aaacaaaaaa tgtttatttt tttcgttttt atgttattat
     7081 cctaatgttt cttgtgtttc tttttcattt cataaatccc tttgacagcc actcgtaacc
     7141 tatcatatat tacgaaaccc ttatctctat ttattgcaaa tgttttattt aaaacatttt
     7201 ctattttgtt aatttttgtt ttttccaatg tttatgtttg ttttgtttga tttttttatt
     7261 gatattatgt tatgatacct ttaatagtta taccctaaga cactgatagg agataagaat
     7321 gaaaaagaaa gagcgtcatg aaaaaattct cgatatctta aaagttgacg gttttataaa
     7381 agtaaaagat attatcgatg aaatgaacat ctcagatatg accgctagac gagatctcga
     7441 tacattggca gataaaggat tacttatcag gacccatggt ggtgctcaat atttggatta
     7501 ttcttcagca aaggacgaag gtcacgagaa aacacacact gaaaagaagg tcttacagac
     7561 taccgaaaaa aaacaaattg cacaaagagc taaaaacatt attaaagatg gcgaaactgt
     7621 ctttataggt ccagggacaa cattagagca tctagctgtc gaattaaaaa atagaaatat
     7681 cagggtcatt acaaatagcc ttcctgtttt tttaattttg cacccgagca ctactattga
     7741 tttgatttta attggtggtg aatgtcgtga aattactgga gcttttgttg gtgctattgc
     7801 cattaataac ctagaaacat tgacattttc aaaagctttt gtcagcgcaa atgcagttta
     7861 caataattct attgcgacgt acagtgatct cgaaggagaa gttcaaaggg ttgcattgaa
     7921 taatgctgtt gagaaaatcc ttctagttga tagtaccaag tttgaaagtt atgacttcta
     7981 taatttctac caactagatc aagtggactt aattgtgact gataaaaatg tgacaactga
     8041 tatcatagaa aagtacaaga aattctcaaa aatcataatc gctgacaagt aaaagaccta
     8101 ctcatattaa aaagcacttc agggtttaga tatatcccct gtcaagtaaa cagggagcat
     8161 atctgaaagt agtgtttgtg ttataaagaa gtttcttgtt aacttatttt ctggtttttt
     8221 tcagacttat cagtcttgta ctgttccttt ttctgatgaa taacgtggaa ataggcagac
     8281 agctatatat ttcacctcta gaatggttgc cattactgtc atcaagacat gcttgttctt
     8341 tatcgtatgg agtgaagctt tcttttagac atcaaaatac ctcttaagta gattttggtt
     8401 cgttaccatg tctacttaag aggtgtcttc aaagcgcttt aaaacgttga tataacagta
     8461 tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg tttttggttc
     8521 actccattat ttaaagtcaa atccctttaa aatcaatgtt tctaaaatga aaaatcacta
     8581 aaaatcttat ttatagacaa atacttaaac ctaaacggta tcaaaaataa gtttcggagc
     8641 ttatcaaata atgaaaatct gtctcaatta ccgagtcttt tttattgact tttttgtata
     8701 tatgatataa ttaaataaat tacataaagg ggttacataa aatgactaca gtaaaaaaaa
     8761 acagagcggt tacctttcaa gctaataaag aattggtaag cgaagcaatg acagtattaa
     8821 acaaaaaaaa tttaacctta tcatctgctt taagattatt cctacaaaat gtcgttgtca
     8881 caaacgaggt tgacttattg acggaagagg agttagagaa agaaaaactt ttcaagcaat
     8941 ttcaagctga aatcaacaaa aatattgagg atgttcgtca agggaagttt tatacctctg
     9001 aagaagtgag ggctgaactt ggactataag aaatatcaga ttatctatgc tcctgatgtt
     9061 ttagagaagc taaaagaaat tcgtgattat atttctcaaa actattcctc aacatcaggt
     9121 cagcataaaa tggagcaaat cattagcgac atagaaaaac tcgaggtttt cccagaagtt
     9181 ggttttgatg ctgatgaaaa atatggttca aaaatcagcc attatcacag cactaaaggt
     9241 tacaccttaa gtaaagatta tattgtccta taccatatcg agggggaaga aaatagggtc
     9301 gttattgatt acttgcttcc tactcgaagc gactatataa agttattcaa ataacacgtt
     9361 aaaagcctta gcagtcttgc tagggctttt ttggtagatt acaaattaac taggagcaaa
     9421 atgagttcgg gctgtttagt agatattcca caaagaagcc aaagactacg aagctaggct
     9481 aatctacctt aaggcttcag gaaacttgga ggagttcacc aagccaactc ataaaactta
     9541 tagcgagata ttcgagaaat ggtatcaagc ctatcatgat acggtagagc caaccacggc
     9601 ttctcgtact cttgatctgt ttcgacttca cattttgcca gtcatgggag aattgcccat
     9661 taacaaaacc tcccctttag actgccagaa attcatcacc gataaagcaa agacctttaa
     9721 gaatatcaag caaatcaagt cctacactga aaaggtcttt gattttgcca tcaagatgaa
     9781 actgctcaag cataacccta tggctgaaat cattatgcca aagcggaaaa aaatacggat
     9841 agaaaactat tggacggttc aagaacttca ggagttttta gccatagtac ttcagaaaga
     9901 gccttacaag cactacgcac tatttcggct actagcttat agcggcctta aggaaaggcg
     9961 agctatgcgc tcttaaatgg gttgatatag actttcagag tgagacacta agcgtcaata
    10021 agagcctagg acgattagac ggaaagacaa tcgaaaaaag cactaaaaat gatttctcag
    10081 taagaaagat taagctagac agcgaaacta tttctattct gcaagaatgg aaagccgtca
    10141 gccaaaaaga aaagacacaa ctagcagttg cacctctttc aattaagtct gactttatat
    10201 ttacatactg tactagagaa gggagtattg aaccacttca tgcagactat attaacaata
    10261 tcttatcaaa ggtcattaag caacatagtc ttaaaaaaat aagtcctcac ggcttcaggc
    10321 atacccatgc aaccctgatg attgagattg gagttgaccc agtaaataca gccaaaaggt
    10381 taggacatgc tagtagtcaa atgactcttg atacatacag tcatgctact gcagtcggag
    10441 aagacaaatc aatcaaacaa tttgctaact atctcaaaaa taactaaata aacgtattaa
    10501 accaagtcaa aagttttccg aaacggtatc aaaaaaggta tcaaaagcaa aaaacgacaa
    10561 aaaaagcacc ttgcaagaga cttgcaaaat gcttgtaatc gttgatgtaa agctgttctt
    10621 aacgttttga gaattgtgaa gctttacgag ctttcttaag acctggtttt ttacgttcaa
    10681 ccatacgtgc gtcacgtgta agaaggccag cgcgtttcaa tgaatcgcgg aagtctgggt
    10741 ctacttgaag aagcgcacga gcgataccat gacggatcgc acctgattgt ccaccgtaac
    10801 caccaccaac aacgttaacg aaaacgtcgt atgaaccttc agttgatgta actgcaaaag
    10861 gttggttgat gatcaaacgt aagtcagcat gtgggatgta ttcttctaca tcttttttgt
    10921 taacagtgat tttaccagta cctggaacca aacgaacgcg tgcaacagcg tttttacgac
    10981 ggccagtacc tgcatattgt gcttgtgcca ttatttaatg ctcctttcct cttagataag
    11041 tcctgagatg tcaagtactt ctggttgttg tgctgcgtga gtatgctcac cgccaacgaa
    11101 gactttcaat ttcatacctt gtgcacgtcc aagagtgtta tgtggaagca tgcctttaac
    11161 tgatttttca atcaagcgaa ctgcgttttt agagcgaagt tcaccagcag tgattgattt
    11221 caaaccacct gggtacattg agtgagtgta gtaaacttta tcagttgctt ttttaccagt
    11281 taatttaact ttttcagcgt tgataacgat aacaaaatca cctgtatcag tatgtggtgt
    11341 gaaagttggt ttgttttttc cgcgaagtac gctagcaact actgcagaaa gacgtccaag
    11401 tggcacatca gttgcgtcaa caacgtacca tttgcgttca acttggcctg gtttagccat
    11461 gaaagttgtt ttgttcatga tttctcctat acgaattcgt aatatttgtt tacaaggtga
    11521 tgggtgttcc gtcccatcga aggtatttgg aaggttccgg ggcctttcaa atggggtaaa
    11581 caataccgcc tactatgata tcaaacttaa gaggctccag tcaagtgctt tagctgattt
    11641 tattggatta ttttttcttt cgatttagga gaatggcaga gctgctctgc tggtgttaac
    11701 gagaatcatc agcttcccaa aaaaggtcat ctaaagtctt atcaagcact cgacaaatgg
    11761 caatgcaaag attaatcgtt gggttgtaat cacctttttc aacagccgca atggtttgtc
    11821 tggagacgcc taccaaatct gctagagctt gttgagacaa atctttgcct gcacgcgctg
    11881 cttttaattt gaggttcttc attcatctgc ctctttagca tccagataat gacgatagaa
    11941 aatactagct cccatactaa ggttacaata tgtcgtttga actgccaacc ctccccaaag
    12001 agccaatgta aagaggaagc ttgcagatac cagtccctct gtgaatcggc taataaagag
    12061 tgacagccag ataaccacaa tcataaacca aaacccaata gtgtatcctt tgagaagaat
    12121 ctgtttttgg cgttcatcat atttgggcaa cttctgatcg tcccttacct tagaatctag
    12181 tacttccttg ctgtctagtc tcgtttggag ctttttcgct gtctttagcc cccataccaa
    12241 caatatgaga aagctagtca atacaaaagc tacgcctcca actaagtaac gttgaaaact
    12301 ggtcttagcc agtaatgttg ctactatcac aactagatcc atcactgctg ttatgacaat
    12361 gaaaccccat tgtgacttag cgagattttg tttgctatca aacatgattc gtttctcctt
    12421 taaatcttta tatgacataa tataaattat tttggatatt ttggatattt tgtcaaatat
    12481 attttacata attgattgac ttaagcagca aaaaaggatt gaggatttct cttcaatcct
    12541 tataagggat taacgggaat tctgcccaat cacaaaaact gccagggtgt taggaccaac
    12601 gtgagctgag ataactggtc ctagtggcat cataagaaca tcgctaatat tctcatgcgc
    12661 cagcagctct tctcgcaact tttcggcact accctgatcg cttgtataag agacaataac
    12721 cgtcgaatcc gcaatatctt tttccacttg agccaccatt tctttgatgg ctttttggcg
    12781 tccccgaatt tttgcaatgg gcaccaattt cccttcctca tcaatccaca aaagaggttt
    12841 gatactggct aaactgccta gaaaagctga accttttgac agacgcccac cacgcatcaa
    12901 atgaaaaaga tcatctacta agaaataggt gcgcagtcga ggaatcactg cttcaacaat
    12961 atctttagtc tccagtaaat ttttgccact atctctggct tctgctgcta aaatggttag
    13021 atagccttct cctcctgcag cagccaaggt atcaacaatt tcaatcaccg catcgggata
    13081 atcttctcga acaagatcgc gtgccattag agcgctttga taggtacccg ataaaaccga
    13141 agaaaaagca agataaagca gtgctttgtt gttcttagca tgttcacgaa aaaccttctc
    13201 aaactctccc acattaatct gactggtttg aggatgactg ccagctttca tctttttcaa
    13261 aaggtaatca ctactaatac ggttaggacc gaccgtttca taaacctctc catcgcacaa
    13321 aatcgttaat cctataagga caatatcatg atcttctgcc caggtttgat tcaaatcagc
    13381 ggttgaatct gtcattattg taaaggtcat tcttacttct cctcatccag ttttttagtt
    13441 tgataatcaa ataattattg ttttatttta tcaaaaaaac ccgcacttgg cgagtcgtaa
    13501 ttctcattat cgatctaact gagacataaa atccttcaat tgaataagct gtccttggtc
    13561 aaaaggccgc agtttaggcg ttttgagatc aatatctcta gacattttat ctaaatcagc
    13621 tttgacagta tgaactcttt gctctaattc tcttgcagtg acacgaagag ctccttgaga
    13681 aaaaatcgtc cattgttcat ttaaatcact cgtagctact tctaccatat gtatagccgt
    13741 atttaactct gctgccatgc gctcaatgta gctatctgct gtttcgtctt cctctgtaaa
    13801 taccactgag atataatact ggtcatatcg ttgccttaac cccggtacat attgggcatc
    13861 aaaaacacaa ataatattaa tattctcaaa atgggcataa tgattaagtt ttgttaaaag
    13921 tgtgttacgt gcctgatcaa gctgatttgt cttaaacaac tgacgggttg attgccaaaa
    13981 ggcaatcata ttatacccat ctactaataa tatccgtttt ttcataattt agcgcctatt
    14041 tcggaaaact tcatacatga gaatagctgc cgctacgcta gcatttaagc tctgtacgtg
    14101 accattcata ggaatcgtaa tcatttcatc aacttgcttt ttgatattgg tagagatgcc
    14161 tttgccttcg ttaccaatga ctaaagcaag tttaccatta gtgttccagc aatcagacgg
    14221 tgttccattc atgtctgtgc caaaaatcca gaatcctctt gatttcaatt tatctagagt
    14281 ttgactaagg ttagttactc tcgctatcgg aatatgttca acagcacctg tagacgtctt
    14341 tgaaaccact ggagtcacac caacagaacg atgtttagga ataatcactc cacatacatt
    14401 tgtagcatca gctgtccgta aaatcgatcc taaattatga ggatctgtca gaccatctaa
    14461 aataagaatc aaaggatttg cttcttgctc tgctatttcg aggatctcat caacatcagt
    14521 ataggcaaaa gctgatactc tcagaacaaa accttgatgc acagctccat cagtcatttg
    14581 tgacaaggtt tttttaggcg tccatgaaat agcgaccttt ttttgtgtcg ctaagctttt
    14641 gatgttatcc actttctttc ctcttaaatc ctcttggata taaagcttat ttcctgtatt
    14701 tgcttgaagg ctttctgtaa cggcatgaac accatagacg atatcgtttg tttcaatagt
    14761 atctttatct tccatgagtt tattatacca tgaatgcttc aaaataaatg catagactct
    14821 tttattttcg tctagatact ttttgaatag ctataaataa acctgcagct aaactgacta
    14881 ctagacctaa taagacccaa gggaatcctc tttttgaagt aggttttact tcctctttgt
    14941 ccactagcat ttttgtcttg ttgctaccgt ttttttggct attactagca gttgatgtaa
    15001 gtttctcatt tttagtagat cgctttaagg aagttgatcg atgattagta gctgatctga
    15061 aaggatttga agtagaatct atttcttggt tagctgattg atttgtttga gcttttgtgt
    15121 ctacatcgac cgaattcgtc gttgctttag aagtatcctt ttgttgcata ttagttggtg
    15181 tttttctagc tttgttttga tcaaaatgaa cagttcctga ttgaccagtt actgcctttt
    15241 cttgagtatg aacggtgaca gttacatgat agtcaccact cttttgtcca gttaaatcaa
    15301 accaagctgt aaaacgacgc tgttgcgctg ccatagtata tacccaacta ttaatagtat
    15361 tatcactagc gtctttaagt gttatcgttg cctgagttgg ttgtctgaca ggttgatctg
    15421 tggtgactgt agcatcaata taaggttctg ttccacgcat actggcttca acactgacct
    15481 gactaatagt tgattcagct ataacattat cagtgtgctg atcttgtgca tacacagata
    15541 tggctttgcc agaagtcccc agcaaaccaa taagtaaagc tagtgataat aagctaactg
    15601 ttttttgcat agttgtcata ataaatatct cctctttaaa ttagtcttac cctctcatta
    15661 taaactattc gaaaatgatt tcattatttt tatgtctttt ttgttacaaa tgtatctttt
    15721 ttatatcatt atgtcttttt gatgtcataa aaagagttaa tggctctcta cctttacttc
    15781 agacctatga aaataagagt ctcctagggg actcttatga ggatatcaat tgttgctttt
    15841 ctacatactc aatacaccat ctgatcaatt cttctaaccg ctctttttgg cccatcatat
    15901 caagataacc cataatggct tcaaaacctg tcgacatacg ataggtaata atatcggcat
    15961 tcttagcttt agtatggcta ttggtattgc gaccacgctt atagatgtct tcttcttttt
    16021 cggtcaatag ctgcgcttct aacatagcct gaatcaagtt ggcttgtgcc ttagcagaaa
    16081 catatctcgt tgctaaacgg tgtagctggc taggtttcgt tttaccttga aaaatgagat
    16141 gacgacgaac atagtaggaa taaaccgcat ccccttcaaa ggctagggca atgccattaa
    16201 tcaaattcac atcaactgga ttagtcacgc agccacctca caccatcttt ggtatctagc
    16261 agcttgatgc cttgagctgc taactgatct cgaatggcat cagctgtggc aaaatcacga
    16321 ttcgcgcgtg cttcttgtcg cttagcaatc aaggcttcaa tgtcaacctc aagcacctct
    16381 tcttcaaaga taataccaaa aacagctaac attttctcaa aagcgctttt aacaggttct
    16441 gtataggagc cagaattaat ccacttggcc atgtcaaata cgacggtaat cccatttgct
    16501 gtattgaaat catcgtccat agcgtcttgg aaagctatta caaactgttt tagctcttgc
    16561 tcatctgcag tttctgtcag cggttgttgc aaggtgtttt taagatactt cagattgatt
    16621 tccgcatcat gaagagcctt ttcagtgaag ttaattggtt ttcggtactg ttgtgtcgca
    16681 agaaagaatc gcaaaacctg accatcaacg gtttgtaaca tgtcatgaac ggtcacaaaa
    16741 ttacctagtg acttggacat tttttcgtta tcaacagtga caaacccatt gtgcatccaa
    16801 tagttaacaa aagtcttgcc tgttttggcc tcagattggg caatttcatt ggtatgatga
    16861 ggaaattcca aatcagctcc gccaccatga atatcaatgg tatcaccaag aatttcagta
    16921 gccatcacag aacattcaat gtgccagcct ggacgaccaa acccccaggg gctatcccaa
    16981 gaaacctcac ctgccttagc taatttccaa agagcaaagt ccagtggatt ttcttttaaa
    17041 gctgtctcag catctgttcg accactggct ccaacttcaa gttctgacag ggttttatta
    17101 gctagcttgg cataatgctc tgacttttcc acgcggaaat acacatctcc agctgcttcg
    17161 taagcaaaat ctttttcaat gaggctttca acaaatgaaa tgatttctgc tatataatcc
    17221 ataacacgag gattttgtgt ggctggctta acaccaagtg ccttggtatc ttctataaaa
    17281 gctgcaataa agcgatctga caattcttta ggagaaacac ctgcttgagt agcagctttg
    17341 ataattttat catcgacatc ggtaaaattg gaaatgtaat tgacctggta gccagtatac
    17401 tcaaaataac gtcgaatggt gtcaaaagcg accgctgatc tagcatttcc aatatgaata
    17461 taattataga ccgtcggtcc acaaacgtac atattgactg tattttcagt caaaggtaca
    17521 aacttgcgga gcgaacgggt catggtatca taaattttaa tcatgtaaac tccttattca
    17581 atagcctggc attagttgtc atcttgatat cctaagtctt tttctgaaat gctgttccac
    17641 cttttcaaca atttgatatt aataaccaaa cctattaacc aaaatagggt caaaatgaag
    17701 tatataatcg ctttaaccat gtcttgtctc agtaaaaaaa ttagactagc caccagtgta
    17761 atactaaaaa caaggataga caaactcata atgacataca gcccaactct tattttattc
    17821 atgaggctcc tttattaacg aaaaaccttc acaacctgtg aaggcttcag aaagtagaca
    17881 aacccacttt tatgctcaaa aagcatgaat agatctgctg atttgacatc aggtttccta
    17941 tttctgtatg tggggtgagc caaaacagct gctttttgtt gtggtaaaag tggcatcaat
    18001 actgacagtg atgcccatac ggatgtttga gaccttaggc tcaaaactaa ggcatggaat
    18061 tccaaaggaa gtcgctgccg taagcaccac ctgagaaaaa atatcacaat agactgtttc
    18121 ttcagtctaa gacttgctta gttgatgctc ttttacgcgt tttttgtatt ttgtacattc
    18181 ccatatcact acactcagca accaaactga tagggctagc taagttcaac attactctac
    18241 tagaaagtcc caaaatattt tattggaagg catcagtgct tctttttgtt attttccgat
    18301 cattttgaca actgataaga gacctctcgt tgtttttgta aactttgaat ttgacgatta
    18361 tctttttgcc catgcactcg tactatctta gctggcacac ctacaactgt cacgtcttca
    18421 ggaacatctg ataacacaac agctgctgcc cctactttag catttgctcc gatgtcaata
    18481 ggtccaatca cttgggcatg tgccgaaatt aaggcacctt gtcgaatcgt tggatgacgc
    18541 ttgccacaat cttttccggt tccacctagg gtcaccccat gataaagcat cacacctttt
    18601 tcaacaattg ctgtctctcc aataacaaga ccagcaccat ggtcaatgaa gactccagga
    18661 gcgatttgtg ctccaggatg gatttcaatt tgtgtccaaa atcgccaaaa ttggctatgc
    18721 attctggcta gtaatttaaa atgatgtcgc caaagaaaat gagacaaacg atgagcagct
    18781 agggctttga tacctggata agttaggata acctcaagac tattacgagc agctgggtct
    18841 agagctttaa caatagctat actttctttc caccaaccca tactaactcc tacgacttat
    18901 ctaattctga ccaatcaaca agaatttgtt ggtcatcaaa aggattgatg gcgacaccga
    18961 acgtttcatc aagctcgttt tcaccatttc ttggttgata aatatcatca atcgtccaag
    19021 tcagaatcac tccttcagct ttcctgaaaa ggcctccaaa atcgtcttgg ttgtaccatt
    19081 tcgcaaagct ttgaagattt gaaaaggcgg gaacatagct ttttccttca ggcgttgaca
    19141 tggtaggaaa aaggcggtcg taatggttat tatctttagg ataaacaaag gcaggaacaa
    19201 ggtaaacctt ttccatcgta tctgccgcca cattgtcatc actcataagc gtatttaaca
    19261 cagtggtgta atggttcata aattgaatca tatcactgct tttgaaaatg gtagaattcc
    19321 caaaatctcc ctttttctta aggttaaaga caagacctgc tgcaccagat gtaatgactt
    19381 cttctagcac tgcaagagct gaacgctcca gccaatactg actttgagca ctctcttgtt
    19441 cttctttaaa gcgagccata tcttccttat cggtaaaaac aggggtaacc ttgacacctt
    19501 cgacttctat cacgtagggt tctttagcag cccagacagg aaaattatgg aaagcattga
    19561 caagggcaac actatccaaa aagttatcag gcgcattgat aaaagctctt aagcgaatat
    19621 ctaattcatt tgatttagtc atgtttttct tcttttttct ctggttttgg tgggcgtgga
    19681 atcaaagctt taattgaggc atccacacga cccttctcat caatcttaat gaccttaaca
    19741 tcaacgtctt cacccacttc aaggacatct gacacgttgg tcgttctagt ccaagcaatt
    19801 tcagaaatgt gaacaagtgc atctgttttg tcaaagaggt tgacaaaggc accaaatttc
    19861 tcaatacgga caaccttagc atggtaaact tcgcctacct tagcttcacg tactaggcca
    19921 gcgatgattt ctttagtgcg gttaatggca gcttggtcac ttgagtagat agagacattt
    19981 ccttcgtcat caatatcaat tttgacaccc gtttcggcaa taatcttatc aatggtttcg
    20041 ccacctttac cgataaccac tttgattttg tctacgtcaa tcttgatggt gtcaattttc
    20101 ggtgcagttg gggctaactc aggacgtggt tcagcgatag ttgcttcaat cacatcaagg
    20161 atttcaaagc gagctttttt agcttgtgca agagcttctt ctaggatttg aggcgtaatc
    20221 cctgcaatct taatatccat ttgaagggct gtaatccctt cacgagttcc tgccacttta
    20281 aagtccatat ctccaaagtg gtcttcaaga ccctgaatgt cagtcaagac agtataattt
    20341 gtcccatcgg aaatgagacc catggcaatc cctgccacag gagccttgat tggtacacca
    20401 ccagccatca gagcaagggt accagcacag atagaagcct gagaagacga accgttagat
    20461 tctagaactt cggcaactaa acgaatggca taaggaaatt cttctaagct tggcaagact
    20521 tgttcaagag cgcgttcacc taaggcaccg tgtccaattt cacgacgacc agcagctccg
    20581 taacgacccg tttcaccaac tgaatattgc gggaaattgt agtggtgtaa aaagcgtttt
    20641 ttgtactctg gagccagacc atcaatgatt tgagtttctc ccattggtgc caaggtcaaa
    20701 actgatagcg cctgagtttg accacgtgta aagagacctg atccatgtac ttttggcaag
    20761 aagtcaacca cagcatcaag cggacgaatt tcgtcaatct tacgaccgtc aggacgaatc
    20821 ttgtcctctg taatgaggcg gcgcacttca gcatgctcca tttgttcaag gatttctgcc
    20881 acgtcacgca taatggttgc taggttttcg tcttctgcgt aacgctcttc gtactcagcc
    20941 ttaaccattt ctttaacggc ttctgtggct gcttcacgcg cttttttctc ttcaacttga
    21001 acagcttttt gcagttgagc attgtactta gccacaatat cagcttgtaa gtctgcatca
    21061 acctgtaaca gttcaacctc agctttctct ttaccaacaa ctgctatgat ttgttcttgg
    21121 aaggcaatta attcttgaat ggcttggtga ccttttaaaa gagcctcaag catgatgtct
    21181 tctgataatt ctttggcacc agactcaacc atattgatgg cttctttgct accagcaacg
    21241 gttaattcta agagtgatgc ttccatctgt tctttatctg ggttaatgat gaactcgcca
    21301 tcaatatagc caacttgaac acctgcaatc ggtccattaa atggaatatc tgagattgac
    21361 aaggccagag atgagccaaa catcgctgcc attggagcgc tagcattttc atcataagaa
    21421 aggacagtgt taatcacttg tacttcgtta cgaaaacctt cggcaaacat gggacgaatc
    21481 gggcggtcaa tcaagcgagc tgtcaaggtc gcatctgttg aagggcgtcc ctcacgcttc
    21541 atgaaacctc ctgggaattt accagcggca tacatttttt cttcgtaatt aacttgaagg
    21601 gggaagaaat cacccgttgc cattttctta gacataacag ctgcggtcag gacggttgat
    21661 tctccgtaac gaacaacggt tgccccgttg gcttgcttag cgacttgacc aacttcaaca
    21721 acaaggggtt tccctgcaaa tgttgttgta aaggtttgtt ttgacatata ttctcctttt
    21781 aattttcaga ggggtaaaga ggtacctgtt ctctgcttag attcagttgt tatacgttgt
    21841 tttgctacaa aaagcaacct gtcaggcttg tcacaagctc ctctttgtat caaacgacgt
    21901 atctgctaca ttttaccata aaatagcaga aatttcactg tcagatcgca atctcttgaa
    21961 atgtccttga aaaatattaa acgaaactta tactctttaa caaagcaaaa aaagcaggtt
    22021 ggtatacctg ctttcgtctc ctaatattaa tgtttggcat cctctaaaat tttaaagccg
    22081 aggctggtac tgtctttcag aaacatttgc acggtttcaa gggaatagtc ttctaaatct
    22141 tttccttgaa gaggcaactt ggcaatcaaa tcagtggtac aagtgataat gtccacgcct
    22201 agctgatcag cttgatagat attataagtt tcacgaggac tagcccacaa caactcgacc
    22261 ccttcttttt gacgacaaat gcgtagagct tcttccatga ttggcattgg gtcaacgccg
    22321 gtatctgcaa tacgaccagc aaaaacagac acaatagctg gcactccagc ggttaaatga
    22381 tcaaccacag cttgagtctg ctcaattgta aaaatggctg tgacgtttaa cttaatgcct
    22441 ttggctgaca acttttgaat gaggggacag gttgattctc ctgttgaagt ggtgactgga
    22501 attttcacat agacattgtc acctagactg gcaattttct cagcttcctt ttccatgctt
    22561 gcgagatcat ccgcaaagac ttcaaaggac actgggtagt ctgtaatttt ggcaagcact
    22621 tctttggcaa agccaatata agaactgatg cctgcttttt tcatcaaact tggattggtt
    22681 gtaaagcctg tgaccaaacc tgtttgtaaa tcctttaaca tggtctctaa aaccgcaccg
    22741 tctgaaaaga ctttaacgtt tagttttttt gtcatcatat cctcctttta gatttcttct
    22801 gaaacattat tgatggcctc ttgagatttt gacagaaagt ttggaagcac caatacaata
    22861 gcaagcacag caaccaccaa gtaaataccg atttgaccaa tcgacgttcc taagcggccc
    22921 agtaaaatcc ctaaaacacc gaaatccaca tctccaaagg tcgtgtttga aaaaccaagt
    22981 ttaccaagta caggtagaag catggctggc aagaaggcga gtaataagcc attagcgaaa
    23041 gcaccaatca tcgcaccgcg acgaccacct gttgaatttc caaaaatccc tgctgttgca
    23101 ccgcagaaga aatgaggcac catacctggg ataatcagaa caccacctgc aactcctaaa
    23161 atcaacatcc caagtaagcc accaacgaaa cttgacgcaa aaccgatgat aacagcggtt
    23221 ggcgcataag ggaagaatac cgcacaatcc acggctggaa tagcatttgg aattaatttg
    23281 ttagcaatac cttggaaggc tggaatcaag tcagccaaaa tcatgcgaac accggcgtag
    23341 acaattgcca caccaaccgc aaaggtaaga ccactcttaa tagcaaaaat aaatgggttt
    23401 tgacctgctg ctaattcttc tgttactgaa gcattcctca aaacagaagc cactgttgcc
    23461 accaagtaga aaatcaccat gataagtcct gttgaaatgg tggtgttgcg taagaaactc
    23521 catttttcag aaatctgaag gtcctcggta tctttactgt ctttgccaac cttgctacca
    23581 acccaggcag aaaggtaata gcccaaacta ccaaagtgtc ccatggcgat ttcatctccg
    23641 tcagtcactt tcaacgtata ctgttgacca atggctggcg aaatagctga ccaagctccc
    23701 aagaggaacc cacctaagat aatcaaaaga cttcctttga aaccaacagc tccaagcacg
    23761 gcggacaaga gacaagccat aaagaagctg tgatgtcctg ttaagaaaat gtatttgaag
    23821 cgggtaaaac gagcaaaagt caaattgagc aataacccaa ccaccaagat ggacatggtt
    23881 tccacaccca gaatcttttg agcgacagaa gtgacggctt cattatttgg cactacgcca
    23941 gtgatgctaa aaccgtgctc aatgagtttg gcaagagggt ctagattggt cacaataaca
    24001 cccgcccctg cagcaagcat aaggtatccc aaaatcggcc ccaaggttcc tgtcaacacc
    24061 ttatgagcag gtgttttcaa agccaccaac cctgcaaagg caatcaagcc cattaaaaat
    24121 gcaggttctt ttaaaatatc tcgaataaat gataataatg cttccatggt atcttcctat
    24181 ctcctttaaa ggcttattcc ttgccactca tttccttgag aaccggaacc aaatcttctt
    24241 caattttttt acgattggta tagcttttaa cgatacaaac cttggcatcc tctggaaaaa
    24301 tatctttaaa ttctttaacg gtaacaaaca aatctgcatc ctttccaaca gccgcattgg
    24361 aatcacagga ttctgcatcc acatcaatac ccaaactaga tgcgatagct tctactttca
    24421 tgcgaagtaa caagctacta ccaataccgt ttccacaaac cgttacaatt ttaatcatgt
    24481 gattcatctc ctttttcaat catatggcta atcagactca ttatttcctc agtattctta
    24541 gcctcaatta attgctcaat atgttcatca tcatctaaaa tcaacgacag ttcctgcaaa
    24601 gccttcaaat gtgaactaga atccacagca gacaaaacaa aaatcaattg cacttgcttg
    24661 tctggatcac cttcttgctt caaatcaaag ctgacaggtt cttttagttg taagagagac
    24721 atgcctaact gccgtgctcc tttttcaggc gccgcatgag gaacagccac cttaggggct
    24781 aaaacaatat aggccccaag ctcattgacc gaatcaatca tgccgtcgat ataagatgtt
    24841 tcaatcatct ggtgttctag taaaggctga gcagccaaac gaatcgcttc ctgccattcc
    24901 gaaacagcat cagcctgcct aataaaatct gctgttaaca agtccaatag tcctagacct
    24961 cctttttcaa tcgttaaatg gttaccaatc aggtaacggg ataaatcact aacaagtcct
    25021 tctttattag taatcgtggt atgtttatga ataatactca acaggtcatc caaagcgaat
    25081 tgctctgaca agggaagatg aaagtcatca cagaccatct ttttcaacat cattttctca
    25141 acaggcccca tcaaagcttg cgtcacataa acaggtttag cacagtcaaa agctactgtt
    25201 gaaaaaatca aatcaaagga agctggatcc aacaacttta ttttgtccag ctggtgaatc
    25261 ctaataaact gtagctgtgg aaaaagttcc ttcaaggttg cctccaacat cagagacgaa
    25321 ctaatgccat tagggcaaac agataaggct accagctgat tgcttggcct cttcttaggg
    25381 gcctgcaacc atctcccaaa atgaatggta aaataagcga cctcatcttc attaactagt
    25441 ttccccaatt gtttttctag aggagaaagg ctgcgcttga ccaagtaaaa gagagattca
    25501 taatcttgtt tgatttgttt cttcaaagga ttggtcaagt taatgtcgta gtaaagtcgg
    25561 taataagctg gcaaaaggtg actgtatagg ttctgacgaa gttctggggt atcggtgata
    25621 gacaatcccg tgttaaccgc caccgaattg atgatttcct ccataatgtc ataaataggt
    25681 tgctgaaaga ctaactctaa gtcaccttgg atacaaccaa gtaacctaga ttgaaccaaa
    25741 tatttttctt cggttagact ggggaagttg accaataact ggtccaccat cgtttcaaca
    25801 gctggcgaag ctaaaggaaa agtatccact gcttcagctc ttacattccg tgaaaatggt
    25861 cgatgagcga gaaggcagaa aaaataaatc aaatccctca atttctccga aataaaagtt
    25921 aagtggttct ccctgctcag ttcctccaac gttgctgcca tcacctttgt ctggtcgggc
    25981 aaaccaagtt catgcaataa gtaactgaac acccaagatc ctgatatgac ttgcaaaaga
    26041 gaactgaccg ctgattctaa gagacgccgc aaggctaagg gttctccttc aaaaaaataa
    26101 ccatcctggc gattgtaagc taaagttacc tgaaaggctt gtacccgatc acgcaccaag
    26161 tttaaatcag caataacaga atttctgctt aggcgtaaca agctttccaa gtggttaatg
    26221 gagataaatt ctttagccat catgatgtaa atgataatca tgtccagacg ctcatcttga
    26281 aagagatagt cgtctaaggt aggtagcatg cgattcaaca agtccttttc agtaaggtct
    26341 ggcggataaa ccagctgcct gtccagagca ataaggggta actgatagag tgacaacgtg
    26401 tcattgattt tagcaatatc tgccagtata tgacgtttgc ttgtctttaa tatggattca
    26461 aatgcctcca gtgaatgtcc tttatattta gaaaagagtt gatagtttct gatgagctcg
    26521 tgagatagca tgtcatctcc tcctataatg tcagtataaa atgaagcgtt ttcattttca
    26581 agacaaattg agtccaattg tctctcgtca tttttaactg caattggact caacaggtga
    26641 tatcattgta acaagttcct caaaaggtgg caaaaaatag gcaaatctgg gaattgattc
    26701 cctttttctt catgatacaa caaaagatac ctcatcactg agataccttt tgttgctaaa
    26761 taaaatcatc aactgctaaa aaaacagtct tagaccattc tagccaaatg tgtaagttaa
    26821 caaacaacat gtcaaaaatg aaaaagccca ctgcataagc agaggacttt ttaaacttga
    26881 aattaacgac gaagtccaag agattggatc aactcacggt aacggttaac atctgtacga
    26941 cgtaggtatg ccaacaagtt acgacggtga ccaatttttt tcatcaatcc acggtaagta
    27001 gcgtggtctt ttttgtgttc tttgatgtgg ctgttcaagt ggttgatttc ccatgtaaga
    27061 actgctactt gaacttcaac tgaacctgta tcgccttcgt gacgtgcata ttgagcaatg
    27121 atttcatttt ttttctcttt tgagattgcc ataatatatt ctcctttttt tgcttaatcc
    27181 gagtcctagg attggcactc ctaaaaccaa gaaaaagttg gtttgtcttt cgaccactct
    27241 attctagcag attcataata ttaggtcaac cgttatccta ttattctgag ctctcacatc
    27301 caaaccacct cactaggctt aaaaaggtta tctggtcatt gaatgtgctc tttttgcttt
    27361 aaccaaacca ccataagcaa cagaactagc aaggccaaat agccaaaagg ctgtttttaa
    27421 atcagaataa acctgtgcga ttgcttgcct aataagataa ccagctaaaa gaggtaagat
    27481 ggctgatccc agcacttcag ctgataagcg gtagcctagc atagtcattc atttctttcc
    27541 aataaagtgt tcactgataa tatgaattgc tctagtatta acaaacccaa tccctaatcc
    27601 atgaacacag cttattaacc gatgctacct gcctttatga cataggtcaa gagttgacac
    27661 aaaatgtttg ccaacaaaac attacctata ccgtcaaaga gcgctttgct tcctatatcc
    27721 ttaaagcgca agccaaccaa gaggttcatc tgaactttac attattagca aaccgttttg
    27781 gaataagtga tcgccatctt aagcacgtac ttaagcagct catttttcag aggattatcg
    27841 aacgcatctg cttcaaaacc tgtcgtattg ccgatttaga cgctctaact caattaagtg
    27901 ttaataacta gacctctgag gtatgcagcc cattaactta taatagcaat cactctgacc
    27961 actacctgac ttttctttaa aaaagaaatg atttgtgata tagtaaatcc agttaaaaaa
    28021 aagtaaagga gtagacatgt ctgcacaaga taaattaatt aaacctagtc atctgattac
    28081 catggatgac attattcgtg aagggaatcc aactttaaga gctgtcgcca aagaagtgag
    28141 cttacccttg tgtgatgagg atatcctatt aggagaaaag atgatgcaat ttttgaagca
    28201 ttctcaagat cctgtaatgg cggaaaaact tggtcttagg gccggtgttg gtctagcagc
    28261 tcctcagatt gatgtgtcaa aacgtatcat cgctgtgctc gttcctaatc ttcctgataa
    28321 agaaggaaat cctcctaaag aagcctatag ctggcaagaa gtcctgtata atcctaaaat
    28381 cgtttctcat tctgttcaag atgcagccct ttctgatggt gaaggttgtt tatctgtcga
    28441 tcgtgttgtt gaagggtatg tggttcgcca tgctcgtgtt acagtagact actatgataa
    28501 agaaggacaa cagcatcgca tcaaattaaa gggatacaac gctattgtgg ttcaacatga
    28561 aattgaccat atcaatggtg ttttgttcta cgatcggatc aatgctaaaa acccctttga
    28621 aaccaaagaa gagctcttga ttttagacta acaaaaaagg ccctttagcc tttttttgtt
    28681 agtctaaata gccgctaaaa gagcctctgc ctgagatagc aagtttgctt ttgtctcttt
    28741 tgaaatctca agccttcctg ttccccaggc atcaggattc acagttgctt ttgtaaactc
    28801 tcctgctact gaagttcgga taaacggcaa tagtgcttta aactgatcaa atacttgatc
    28861 atgcccgcca tttgcaactg aagagaccgt aaccacctta ccgccaatag cagatgggcc
    28921 cgtcggatca gacaaatcaa gagcacgaga caaccagtct agcaggtttt taacagaacc
    28981 tggaatagag aagttgtaaa ctggtgtaaa aatccagata gcatccgctg actgaacagc
    29041 ttgacgagcg tcaacaactg gtaaaggtgc attagcttcg atatcttgat tcaaaacagg
    29101 aacgtctttc caatttaagt aagatacaac tgcttgatgt tccagagctt tttgtgcttg
    29161 agccgctaat tgatggttaa aagacccttc acgaagcgag ccaacaataa ataaaatatg
    29221 tttcatctct ttgtcctctt tttttaaaga tcgttttaaa gctttctaaa agtggtattt
    29281 cattgatact tctttttgaa atttatcact atgtagtaat tatatcttct ctaagaagag
    29341 ctgtaaagag atttgtttat aattgtttga actttttgag aagttcaatc agacaaagtt
    29401 gttctttttc agtcaaaaca gcaaaggctt cttccacccg tgcgacgtgt ttaggcaaaa
    29461 cagcttcaat caagcgagta cccttatctg ttaaggtaac cacataggct cttttatccg
    29521 ttttgtcttt gcacttgcta atccagccat tacgttccat attatttagt acaacagtca
    29581 tattccccga agtcgctaac aaagaatcaa tcaagtgatt gattcgcata cagcctttag
    29641 tatatagtac ttcaagcaca ctgaactgcg tggctgttaa atctgccttt ttaaaaatat
    29701 cagccccaaa agcatctagc gttcgctgag ctttacgaaa caccaccatg gctttcaaag
    29761 cggtgttctt atctaaatga ctcatttgat ttcctcgctt tatacggtat gtcacaattt
    29821 agtatcagag agattagaaa gtgatcttgt ccctcttctt ttatgacttt gcatatgact
    29881 accattatga taatctagaa aaagtcatca aaaagactta attgattatc ttctggcata
    29941 tttcctaaaa tacctatctc atccattttc tcaacgagcg ttgacgatgc cccgcctcgt
    30001 ttacgcaatt ccattttaga gaggaattcg ccttcttgac gagctttaac gatttgcttg
    30061 gccacgtttt cacccagacc ttctaacgct ataaatggag ggataagggt atctcctttg
    30121 atttggaatt ctatagcatc acttttgtaa aggtctaatt tgccaaactt aaagccgcgt
    30181 tctaacattt cgttgacaat ctccaaggtt gtaaagaggt cattttccac attggtggct
    30241 tcattatttt tacgttttat agtaatatct tccattcttg ctttaacagc atctaagcca
    30301 ccactcatgg tttttaattc aaaagccttc gcacgaatag agaaataagc acaataatac
    30361 ataatggggt ggtgcacctt gaaataagcc acccgaaggg ccatcaagac ataggccgcc
    30421 gcatgggctt tagggaacat gtacttgatt tttccacacg attcaatgta ccagtcgggc
    30481 acattgtttt ctcgcatggc atcaatatag ccattacgtt cttcctcaga aattttcagc
    30541 cagaggccct tacgcacacg ctccataatg gtaaaggcca ttttcggttc taagcctgcg
    30601 tgcatgaggt aaaccatgat gtcgtcacga caaccgataa cggtttttag ggtcgcaatg
    30661 ccttctttaa tcaaatcttg tgcattacca agccaaacat cggttccgtg agatagccca
    30721 gacaactgca acaattctgc aaaagtggtc ggatgcgttt cgttaaccat gccgcgaaca
    30781 aagttggtcc caaattctgg aatgcctaac ataccagtcg gtgtcccaat ttgttccggg
    30841 gtaacgccca aaatctctgt cccagaaaag agagccataa cacctggatc atcagcaggg
    30901 atagtaatag gatcaatgcc cgataaatcc tgaagtttac gaatcatggt cggatcatca
    30961 tgccctagga tatcaagttt caagacgttt tcatcaatat catggaagtt aaagtgagtt
    31021 gtctgccaag aagccgttac atcatcggct ggatattgca cgggggtaaa atcataaaca
    31081 tccatgtaat taggaacaac aacaatcccc ccagggtgct gcccagtcgt tcgtttcaca
    31141 ccagcagcac ctgctgctag acgatccacc tcagcatccc gatagaactt gccatagtcg
    31201 cgttcatagc ctttgacaaa tccataagct gttttttctg ctacggtacc aactgttcca
    31261 gcacgaaagg cgtattcgtc accaaaaata tctcggacat ccaaatgggc actcggctga
    31321 tcatcaccag agaagttcaa atcaatatcg ggcaccttat ccccgtcaaa tccaagaaag
    31381 gtctcaaagg gaatatcttg gccgtctttt tgataagggg tgccacattt tggacaaggc
    31441 ttattaggta aatcatagcc cgatccgact gacccatctg tgataaattc agaatgttgg
    31501 caggacgggc aaacgtagtg aggcggcata ggattaacct cagtaatccc aatcatggtt
    31561 gccacaaaac tagaccctac agatccccta gagccaacca gatagcctcg ctcatttgac
    31621 cggttaacaa gcatttggga agcgagataa atcacagcaa aaccgttccc caagatagag
    31681 gttagctctt tttcaatgcg taaatcaata atatctggga gaggattacc ataaatttca
    31741 aaggcttttt gataggttaa ttcggcaacc gtctcttcgg ccttgtcaat atacggggtg
    31801 taaagatcgc ccttaaccac ttccacttcc tcaatacggt ccgcaaaatc ctgagtattt
    31861 tgcacaacca cttgataagc gaggtctttt ccaagaaagg caaactcatc cagcatttca
    31921 ttggttgttc taaagtgcgc tttaggtaga ggagcaggtt gggccccttc cccacggccg
    31981 attgttctat taatcatggc accctgaccg agactacgca caataatttc acggtaaatt
    32041 tcttcttcag gctccagata atgcacattt ccagtggcaa gcacaggttt cttagctcgt
    32101 ttccctactt caatgaggtc acgaatcacc tgctcaatac ctgcttggtc tttgattaat
    32161 tcacggacaa ccaatggctg gtaaatggct ggtggcatga tttcgataaa atcataatac
    32221 ctagctaaat caaccgccgc atcaattcct ttagtcagaa cggcatcaaa aacctcgccg
    32281 tcagaacaag ctgtacctag taacaaaccc tctcggtgag catctaagac ggttcttgga
    32341 atacgcggca ccccttcaaa atatttgata ttggaaaggc tgaccaactt aaacatattt
    32401 ttaagaccaa cctgattttg cacatagata gtcgcatgct taatccgcgc ttttttgtaa
    32461 gaatcctcag ccaccaaatc tgtattgagt tgcaaaagat ttttgatgcc atgcttttct
    32521 ctggcatctc ttagaaaaat aaacaaaaga cgtcctgtgg cttccgcgtc gtaattggcc
    32581 atatgatggt ggtctagact cacttggaaa cgcttggtga gcggtcccaa accgtgacgc
    32641 ttgtactcag gatacaagtt tctagcaaat tctaaggtat caatcacagg ctgtgtgatt
    32701 ttgggcaagt cgtggcgttc ataattggcg ttcataaagc ccacgtcaaa actggcgttg
    32761 tgagcaacca agatactatc tttgcaaaag tcctgaaaag cttttaggac ggtaaccaat
    32821 ggcttggcgc cctgcaaatg cttatcggta atccctgtca attcggtggt aaaggcagaa
    32881 agaggatgcc caggatcaat gaattcatca aactgctcta caatatttcc tttaaacatt
    32941 ttggaagccg caatctgaat caggtcatta ttcatagcag atagacctgt ggtttccacg
    33001 tcaaagacca cataggtagc ttcgtgcaaa tccatatcaa caggatcata agaaataggc
    33061 accttgtcct caacaatatt ggcttctagg ccaaaaatag ccttaattcc agctttgcga
    33121 gccctatggt agccatgagg aaaactttgc acattagcat ggtcggtgat agcaaccgcc
    33181 ttgtgtcccc acttggctgc cgtatcaatc aagctttcta ctgtcggtaa ggcatccatg
    33241 gtagacatat tggtgtgggc atgaagttcg acccgctttt gcccttctgg catcaggtct
    33301 ttgcggtcat gatggacaat ttctttgacc tgctggacat tcatggtgag actcttcgta
    33361 aaaggattgg tctcaatatt cccttgtacc cgtaaccaag cccccttagc aatcatatca
    33421 aatttacgga gctcctcatc gtctttggcc catttttgga gagcaaacga ggaggtatag
    33481 tctgtcattt taaagttaat gatatggcga cctgttctag tcgttttacg ctccacgtca
    33541 aaaaccatcc cctcaaagac aatccggttt tcttcggtct caatctctat cattggtgtg
    33601 atggctgctt tttcaaaccc tgcctgacgc ttagctgctc gttccttgta gtcaaactta
    33661 ggagcaggtg tggcttcctc aactggtggc atcatggctt caagggattt ttgggcttcc
    33721 aaattatcct gcacagcctt tttcacaaga gcctgacgac tggaaacaaa atccttggtc
    33781 aaatgctcag tcatctcctg atctgacacc atatctatgg tcaaggtgcc aaagccaaag
    33841 gcttctaatt gcttgaccag attaggcaaa tgattttttc taaaatgatc gttattcaca
    33901 aaacctggcg ctgcaataat gagtttgtca tcctcataag tcactttgag ctttgagaaa
    33961 gaagatttaa aactagcact attacacggt gcatgctcaa aagcttcttg gtaataagct
    34021 tgaagcagat catctgaata atccacctga gcagcttgga tatcaaaggt taccttaatg
    34081 tcagccgcct caaaagttcg cgtcaaacga tcatgtaatt cacgataagt tgcaatcggt
    34141 aaaaccgccg caaaggcaaa atgaaattcc cacaagcgtg acaccgaatg cacctttacc
    34201 tcgataatat cagcagatga aaaggcactt gaacgtctca tgtcaagtgg catttctatc
    34261 tggtccatca atttagcgaa taaatctgac ataattttct cctgatttta ctaggcttta
    34321 ttttatcaca attcaagatg attttcgagt tttagatgga aagaaaagca gggctttctt
    34381 ctgacgttct ttggctaatt tattccaagt gatagctcag ttgaggtcat ttttttgtca
    34441 atcttttatc gccaagcgtt tttggtaaag caaaagccac tctgtaccag acaagttcac
    34501 agcaaaaaga ggccactcta aacaggcagc ctcctcattt cttttttagt gttcttttgt
    34561 taaaatttca agggtttcga tgaggttttc tgcattaact tcaatgctgt cacctgttgc
    34621 cttgatttta atttccacga taccttcagc ggctttttta ccaacagtga cacgaattgg
    34681 caaaccaatc aaatcgctat cagagaattt cgaaccgacg cgttcattac ggtcatctgt
    34741 caagacatca taccctttag ccatcaagtc agcttctaac ttcgccgtca agtcttgggc
    34801 cacttgatct ttgacattaa ctgtaatcaa atgcacgtca aatggggcta attctttagg
    34861 gaagttaata ccccaagcat aacggtaatc gccttttggt gtcttgttca caaagagacg
    34921 ggcatgctgt tcaatgacag cagacaaaat gcggctaacc ccgataccat aacaacccat
    34981 cacaattgga actgttctgc cattttcatc aagaatcgtt gctcccatgc tgtctgaata
    35041 acgagtgccc aacttgaaga tatgaccgac ttcgatacca cgcgcaaact ggagaacacc
    35101 atgaccatcc ggagacattt ccccttcttt gacttcacga atatctacat attcagcctg
    35161 gaagtcacgt cctggattga ctcctgtcac atggaaacca tccttgttag ctcctgcaac
    35221 ggcattggta aggttttgca ctttgcggtc agccacaatg cgactacctt gtgccaagtt
    35281 gacaggccca agtgagccaa aacctgcacc aaagaaagca cgggcttctt cttcactagc
    35341 tggctctaaa aaatcagcag ctagatagtt ttttaattta acggtattga tatggtcatt
    35401 tccaactagc aaagcaacaa caggttcatt atctgccaca aaaagcaagg ttttgatggt
    35461 ttgtgtttca tctactgaaa gaaaagcagc cacttcatca atcgttttgc aatgtggtgt
    35521 ctcaacttct gccaaggcat cttcagcagc tacttttgag gacggtttat attcgttagt
    35581 tgccatctca aggttggcag cgtagcttga ttttgttgaa taagcaatag tatcttcacc
    35641 tgaaatcatc caagcagcca attctgcctt aatctcttct aagacctctt ttggaatatc
    35701 atccattgac gcaatagact tgtcaagaac cacccagcga tcaaggtccg tacgagctgg
    35761 tgtaatggcc ataaattctt gggaatcttt cccccccatg gcaccgccat ctccaataat
    35821 ccctttgaaa tctagaccag ctctggtaaa aatagcttcg taagcttgac gataatcttc
    35881 gtaggtcaca tctaaatctt catagttgtg atggaaacta tagccgtctt tcatgataaa
    35941 ttcacgggta cgaagcaagc cattacgcgg acgtttttca tcacgatact tagactgaat
    36001 ttgataaagg tttaagggca attgcttgta agatttgacc gcattacgca ccaaagtcgt
    36061 aaaggtttct tcgtgagttg gacccaagat aaagtctgag ttatcacggt ttttaagctt
    36121 atagaggtcc tctccatagg tctcataacg gcctgattca cgccagagat cagctgtcaa
    36181 aagagctggt gccaacattt caacagcacc gatcttttca aactcttcac gcatgatggt
    36241 cttgaatttc tcaatggtac gatttgccag tggtaaataa gcatagatac cagcagaaac
    36301 ttggcgcaca taaccggcac gcaccataag cgcgtggctg ataacctggg catcacttgg
    36361 catttcgcgc aaggttggga taagcagttt actttgtttc atagattatt ccgttttaat
    36421 acatcccctt atcctcaaac ccttctggaa acatcaagta agagtgaagc gggattattc
    36481 aataatttga atgtgtattc ctttcatttt gagtaagacc tcaacccttg caaatcttag
    36541 ggttgaggtc tttttcgatc tttaacatga taaggtcagc ttaagatgac ctatcttgat
    36601 tagaaaaaga cgcgcatgat atcattccat gtcacagcaa tcatcaatac gaccatgata
    36661 gcaaccccag ctagggtgat ataggcctct gtttcttgct tgataggctt gcgacgaatg
    36721 gcttcaatga tattcatcaa gatttttccc ccatcaagtg caggaatcgg aatcaggtta
    36781 aagatcccta aattgatcga aagcattgcc ataagagata agactgactc taagccgttt
    36841 tgagcagcct gattggacat gtcatacatg gcaacaggtc cacctaattt attgagacta
    36901 aagccagtga tcaatccttt caaagcattc aaaatagtaa acgccccact ccaagctaat
    36961 tctagaccgc ctaagagctt atccttaaat cctgttttca gactcgcctt aactcctatc
    37021 gtgtatgtct ttgtatgctt ttgtggtttc acagccaccg tttttaagcg ctgatgtgac
    37081 ttgtaggtga ccttaatggt ctgtgacggg cctagatccc gtgttgcaag gtctacagcc
    37141 tcagtgagat cattccagct agtcacctta taaccattaa ttgcgacaat ttggtcatta
    37201 tctcgaagac cagccttagc tgctgctcca ttttcttgaa cacggacatg attgctacta
    37261 aaatctggca tcccaccttg taaaaagacc aagaggataa aaaccacaat ccctaaaata
    37321 aaattattca tgggacctgc aaaattggtg attaaacgtc cgccaatact agcattttga
    37381 tattgaacat caagcggagc aattcttatc tcagtaccgt cttcttcaac aatagtagca
    37441 tcgtgagcga ccttatatgt cttggtttct tctaaaacca agccagtaat actcagctga
    37501 tcttctaagt catagcctgt aacatgcatt gggagactcg ttgggtctaa cttactttga
    37561 gacaagttga tgcgcttaac aaaaccttgc tcattaaggg ttaaacttgc tggagtacct
    37621 gttttgatct cagttttgtc atcaccccaa ccagccattc gtacataacc gcccaagggt
    37681 aacatcctca aggtataaag agttcctcct tggtcaacat gagaaaaaat tttagggccc
    37741 attccaatgg caaattctct tactagaatg cctgattttt tagcaaaata gaaatgtcca
    37801 aattcatgga caatcactaa aataccaaaa ataataataa aggttattat tcctaacata
    37861 gatttccttt cagtctattg cttttttgag ttttgtcgcg ttgttgcgac tccaaacatt
    37921 acctcttaag attaaaataa accaaagaga tgcatgatgg gaaaaacaaa aatcatggaa
    37981 tcaaagcggt ctaaaatacc accatgtccc gggataagct tacctgaatc tttgacacca
    38041 aagtgacgtt tgagagcact ttcaaccaaa tcaccaaatt gggcaaaaat ggaaaaaaga
    38101 gcgaccaaaa ccaacatggt caaaaagtga tgtggcgcat aaacagacct atcaatcacc
    38161 ataaagataa aagacacaag gactgcacaa gcgatccctc ctaaactacc ttcaatggtt
    38221 ttgtttggag aaactgtagg caatagtttg cgtctgccaa actgacgccc aatcagatat
    38281 gctccaatat cagtggccca aacgataaat aaggccaaaa agaccttgtc aattcctgac
    38341 aggcgagcat tgatcaaatg ttgaaaacca atcccaacat aaaaactagt tgcaatcgga
    38401 aaggccgcat catcaaatga ataagctctg ctatttaaaa cagtacctgc caaaatgaaa
    38461 aaaaccatta agctgtaaaa agcaacattc gcatcaattg gtaaaaaagt gaggtaatgg
    38521 tccatcggaa ctgccaatac aaaggcggct aacatggcaa aaacaccttc aaaagaaaag
    38581 acttctaatc ttttcatttt cagtaattct gataccccaa tcatggccaa aacgcctaca
    38641 aataattgga atggtaggtt tcctattatg agaaaaggta ggaaaatagc tactgctact
    38701 cccccccaaa caacacgttc tttcataact atctcctttt agaccttgcc aaaacgacgt
    38761 tggcggcggt tataatccgc aatggccttt agtagctctg cctttttaaa atcaggccac
    38821 aagactggtg tgaaataaaa ttcactgtaa gctgattgcc aaggcaagaa attgctcaaa
    38881 cgcagctcac cactggtgcg aataatcaaa tcggggtctc ggtagagata tggcagatgg
    38941 tctgtcatca agtaattagc aatcaaatcc tccgtaatat ctcctggatt taatttggca
    39001 tctaagacat cttgagcaat aaagcgaaca gcactggtaa tctcagcacg tcctccataa
    39061 ttgagagcaa aattcaaaat taaacccgta ttacgttttg ttttgtcgat ggctgcattt
    39121 agcgctgcca aggtatcttc aggtaaacga ctagtctctc caatcatttg aatctttaca
    39181 ttgttctcat gaagcacagg cacatatttg tcaaagaagg ttactggtaa attcatgata
    39241 aaagacacct catcttgggg tctggaccag ttttctgtcg aaaaggcata gactgtcaga
    39301 actttaaccc ccaactcaga tgcagtaatc gttacttctt gtaaagcatc catcccagct
    39361 ttatgcccaa acactctagg ttttagtctt tttttggccc aacgtccatt accatccatg
    39421 ataattccaa tatgctttgg gatactcccc aaaacttttt ttgttgattt cgcttttaat
    39481 ccaaacatgt tttttcctta ttgataagca tacttatctc atattttatc atatttcacg
    39541 ccttttggct tgttttttat caattagatc ttatcttcac aaaatgttca cctatcctct
    39601 gatagatgtg tcagcttttc atgaacctgt tagcatcaga ataacttcag tccagggatc
    39661 aaaacaaaaa agtttggcat atttcatcca aactttcccc gccttaatgg ctttcaatgg
    39721 cactgtcagt gtcagtgcta tctactgtct caacagcagg ctcattaaca acactggttt
    39781 cttctgtttc aaccagtgtc gtttctgtgg tagcttttgt tacaatacgc ttaatagctg
    39841 atagctcaaa tgtcaaaaag acgccatcca catctaacac aatcttttta gctgttgtat
    39901 cgacttcgtc aacaatcgca aacatgccac caatcgttac gacttcgtct cctttttcaa
    39961 tagcgtttaa ctgattttgg cgttcttgcg cttgtttttt ttgttgacgt tgcataaacc
    40021 aaatcaatcc aagcatcact acaaacatta aaattgttgg aaatcccata atgtctcctt
    40081 tttattctat aaaagtcatg tcttactata tcaaaaatca agcggtatag caagactaga
    40141 cgttacctga tttcttcttt aaacgagacg ctaagcgcag tgctttactc acgagtcaaa
    40201 aaagctaaga tgtcatctgg tgtcaaactt gaatcacata cagccctttg gtgctgatcg
    40261 taactaacca gcaaagctgg aactgtcact aattggtagt tctcacgaaa ggcagcaagc
    40321 tcagcagcat cagcagcgtt ttcgctatcc acaaaataga cgtctttttg attatctgtt
    40381 gccacttgtg ccaacttagg agcaaagcgg cgacaataag gacagctcga acgtcctaaa
    40441 aagacaatca tgtctttgcc actagcaata gctgatgtga cttctgcaac actcgacggt
    40501 ataaaattcg caacgatttc ttcaaatgtc atggatgtct ccttctcttt tggtctcatg
    40561 atacactaaa gcagcaattt tctcaatcac aaggtctttt cacagctcag agcttttata
    40621 gccgtcaaaa agaatccgca aacggcaatc tttttgtctc tattaacacc accctaatct
    40681 ttttggcgct tcatcaacag gctaatagcc aataaaagca gagctactcc agctgctaat
    40741 aagccttttg aggaggcttc acccgttttt gggagctttt ggtttgtttt agctcttttt
    40801 ggcctagtca ttaatgattt gtctaggtct tttgagctat ctggtgtttt ggtttgatga
    40861 ttgtctgtct gactcttttg ctgagaggga ttagccgttt ttgaggatac tttgaggacc
    40921 agggcagtta ggccatcaat agtcaagcct tctttggtaa actggactcc cttaggctta
    40981 gcaatggcag taacaccagc ttgctcagca tcagcaagca cttgggctcc tagcaaatag
    41041 cggtaggctt gaggtaaaac taccttgcgg gtcttgttgt ctgcattgac aaagacagca
    41101 tagcgatctc catttgatgc caccgtttgg taacccatga tgaggtcctc ttgttgaata
    41161 ccatcttgcc ctgcttgggt gatcaaggtc acatcccgat ctacctcagc tttggttgct
    41221 tttgtaaagg catctgttga gcgacgcaac gcaattagtc cttgtgtata ggcttttgtt
    41281 tggttgctaa tcgggtgagc tctggaatct gttgcctttg cccagtcaaa atgattgacc
    41341 gcatcagacg aatcataaga atcgtggatg aagtaagggt attgcgctac agcatcaatc
    41401 agagtcgcct tatttggcac cttgtcatca gacacctttg tcttgtagtc gggatttaga
    41461 agctgcttgg ttcgtccata ttcctgacca gaatggataa aggcagtccc ttgagcagtt
    41521 aaaatcatgg tatttcctag acgaatacgc ttgtgaatct cttcttcagc cactttagga
    41581 tccttattga tggatttggc aatgacatca tgtaaggtca ggttgtcatg ggctgcaata
    41641 tactgcacta catctccagg ggcatctgct tcaaaattac taggctgtgc tttgatcgtt
    41701 ttgaataaac cttctagatt ttttgcgcca ccagtaataa aggctgctgt gccttcattt
    41761 ggaaaacctg atttgagggt atttctgata tcatcagaga aaacaccgac cgtattggtt
    41821 gctttcatcc aatcttgatc tgccgcaatt tcttttttcc cctcatcacc ttggtaggta
    41881 cgccagcctt cgccaatcat aatggtattt ggattaatgg ctttggctgc cttaaaggct
    41941 tgctcaatag ctgccgcatc atggtcaccc atcatgtcga aacgaaaacc atctaccttg
    42001 aattcacgag tcagataagt aatcgaatcc accaagatac gacgactcat ggcatgtgtc
    42061 gttcctagac gacctccgcc aaaactctct ctagctgtac catcagcatt cataaaatga
    42121 tagtagttgg gttccaaatc ttcaaagaga taggttctag ccgtgtggtt ataaaccaca
    42181 tcaaaaataa cacccatacc acgtttgtga atctcattga caaggttttt aagctctgcg
    42241 atacgtaaag cagggtcgtt aggatttgcc gaatacatgc cagaaagggc aaagtagtgt
    42301 tgtgggtcat aaccccagtt gtaattattg tctgaagacg tgtaggctgt tgagcggctc
    42361 ttgtccagct cattggcata aaaataactc aaaaccggta gcaattgaac gtgggtaacc
    42421 cccaagtctt tgagatagtc tagctgttca acgaaagctg aaaaagtccc aaaagggtgt
    42481 gttaacttgc cttctagagc cttatctgac gtaaaatctc gcacatgtgc ttcatagata
    42541 atagcgtctt cacgcttttt aaagttgtta attttggcaa aaccaaggcc tgttggtcct
    42601 agtttgcttg gatcaataaa ggcagctttt gctgttttga tgtcatcagt agcagtcgca
    42661 tcattccagg cagcgagaga tttggcgtaa ggatccaaaa ccatgacttt ttcctgaccg
    42721 cgcgtgattt cataaaggta atagtagcct gtgtaatcac taatgccctt gacactatca
    42781 gaagtcagat gagctctcca aacacccttg tccgacttgg tcaaatcagc ttgaccaacc
    42841 acccttgtct gatcttgttt atcataaacg acaaccttaa cagtatcagc acttggagac
    42901 catagcgcta aatcaacaga accatcctta gctagggtag ctccaagttc accatcgtaa
    42961 gcatagagtt tatcttttaa ttgccaggat tggcgagcga cttggctaac ttctccaaat
    43021 gtaaccgtgt agactgcacc ttgcgcctta aaatcaccct ttaatgtcat tacagactta
    43081 tccttgtcaa gtgtcaactc atcaattgca acagttttcc ctgctttgtc agtgatcttg
    43141 atgttttgtt tcaccgcatc ttcatcaagc ccatctaagg tcgtaaaaat ggctttaatc
    43201 tcatttggcg tggtttgttc agctcctttg agactaacct gatcaatata ataaggattg
    43261 ttgtaaactt ttgggtcagt gtctttgaca aaaacctgag tatgattgtc taactcttta
    43321 aatagataat cttttggttg cactttaata gcatctcctg tcttactttt atcaaggatt
    43381 aaaaatccga tttcgttagc tccttctttt aaggggacat caacataagc tccataaggc
    43441 cctttatgtg acaagtcaag tccatttggc cagtcggtcg ttggggtttt gacatcttta
    43501 aaggtccaga cagctaagtt atcgtagtgt cccgattgat tgtggtagtt gattcgaagg
    43561 taaccttttt tcaaaggtcg ataagcgtgt gcatggtaat tctcgtctat ccaaacttca
    43621 ttcatttttg gcgtgagaag cgagatgtgc tggtcctttg aaagattctc tccagcttta
    43681 ttattaatga gataagacac ttgctggcgg tgtttagctg ctagtggcac atctagataa
    43741 tagccatagt catctttttt cgcttttgtc atggtgatag caccatttgg ccaatccttt
    43801 gaaggttgat ccacatctcc ccacacccaa agtcccaagc ttcctagcga ctcaccagct
    43861 ggcaatgttt taaaatgcat acgcaaatga tgctcagcaa tactagcagg attagctgac
    43921 gagctgtcta aaactggttt agcctccttg ctagacgatt gaaaaccatc atcagattta
    43981 gcattgccat ctccttgttg agcttcatcg cgggctagtc caacaatggt tttagcagca
    44041 acttgatgtg ttaagatact aggggtgcac cctaacacga cagtcccagt tgcagcgatt
    44101 acaaaaccta tcccccactt ttttaacaga tactgatagc gctttgatcc ttggttgact
    44161 ttctttttca taagacctcc atttagtata acattcaaac aagactatct tatcacattg
    44221 cgcaagcgct tgcaagacat gccgcacttc aacttagaac acagctcaaa taatgactat
    44281 caccccttaa taggatctca aaaattaaac agccaaaacg agtttccact cgcttggctg
    44341 ttgggtaaac tcaaatcaca tacaactgct aggccttcag tttgagacaa aaggcatccc
    44401 aaggttgtaa gtgcttggtc tctaataccg tgtccacatc tgtattggtg atgatcactt
    44461 gtgcgcctgc taagtctttg gcaaagactt gctcctcatc agacacattg acaacaatta
    44521 catacgtctc ttctccaagc tgacgctgat aagcaaacac tttatcagct gttggcaaga
    44581 ggtgataatc cgcttcaact agccagtcct gctccttacg caaggcaatc aatttttggt
    44641 aggtgtaaaa aatagagtct ggatcagcaa gggcatcggc cacattgatg tcttggtagt
    44701 tggggttgac cggcaaccaa gtctcttttg cttcagaaaa gccagcatgt gtgtccttag
    44761 accactgcat cggcgttcta gcattgtctc gtcctacctt acggatgcta tccatcacac
    44821 gcgcagctga cactccattt cccatagctt ccttagcata gttaagggac tcaatatcat
    44881 taacctcggt taagtcttta aaaggataat tggtcatgcc aatctcttct ccttggtaaa
    44941 tgtaaggggt tcctcgcatc aaatgcagta aaatagctaa agccttggct gatttttctc
    45001 ggtaagtgct gtcattgccc caaatggata agacccgtgg caagtcatgg ttattccaaa
    45061 aaagcgaatt ccagccttct cccaacttta attttgtctg ccacttgcta aaaatagctt
    45121 tcagagcagg cacgtctaat tctttggcat aatcccactt gggagcgtca ggtttatgtt
    45181 gaagacccac atgctcaaat tggaagacca tggataactc tttattttca gggcgagaat
    45241 actggcgagc aatttcaggc gttgctcccc aagtctcacc gacagtcatc acgtcatgat
    45301 tgccaaaggt tgcttgattc atttctttga ggtaatcgtg taaccgcggc ccgttacctg
    45361 tgatttccaa gtcaggaacc ttcccaatca ggtcaatcac gtccatccga aaaccaccta
    45421 tgcctttggc aatccagaaa ttcatcatat cgtaaatttt atgacgcaaa tcttcattct
    45481 cccagttgag atctggctga cgtttgctaa aaaggtgcaa ataatattga cctgatgcat
    45541 tatcatattc ccaagctgag ccactaaaaa tagaagtcaa atcgttaggt tcatctcgcc
    45601 aaatataaaa atcacgctca ggactattag ggttttccct agcttcaaca aaccaagcat
    45661 gctcatcaga cgtatggttg accaccaagt ccatgataat cttaatgcct cgctcattag
    45721 ctgctgctaa gagctcatcc atatctgcca tgtctccaaa aacatcagca atcgcttcat
    45781 agtcagagat gtcatagcca ttgtcatcca ttggactttg gtaaacagga gataaccaaa
    45841 tggctgtaat gccaagtttt tgcaagtagt ccagttgact ggtaatgcct tttaaatcac
    45901 caataccatt gccattggtg tctttaaagg atctcgggta aatctgataa atcgtagctt
    45961 tatgccacca gtgtttcttc attattcttc tcctattaat cattcattca tcttttattt
    46021 taacgttttt tctcttttgg tgcaatcgtt tgcgcaaaat gacatgataa aaaggttgga
    46081 acccaacctt tttatgaata tcgcttatac cttaacgaat agcttgttcg gtatctctgt
    46141 caaagaaatg ccctttagcc acattaaagg tcaagctaac ttgctcccct ggactatgga
    46201 aatcacgcgc atctacacga gaagcaaact cagtttgtcc aagcttgaca tagagcatcg
    46261 tctcagagcc cagtaactca gaaaccaaaa cttctgctgt gacagttgca cttggataag
    46321 tatcatgcac gatttgtctg ctagaaatgt cttctggacg aataccaaaa gtgacttttt
    46381 tccccttgta accagcagct tcaagcattt ttgcttgacc ttcaggaatc gcaatagcaa
    46441 gaccatcttc actcacaatg cggccatctt taacttccac ttcaaagaag ttcatggcag
    46501 gacttccgat aaagccggca acgaatttat tagctggaag gttatacaat tcttgtggcg
    46561 agccgacttg ctcaatttta ccaatcgttc cattaccttg agggttttta gtggcactca
    46621 tgataacgat acggtctgcc aaggtcatgg cttctgtctg gtcatgggta acgtaaatag
    46681 tcgttgagcc aatcctacga tgaatctttg cgatttcagc acgcatagag acacgaagct
    46741 tcgcatccaa gtttgacaaa ggctcatcca tcaagaatac cttagcatca cggacaatag
    46801 cacgtcccat ggcaacacgt tggcgttgtc caccagacag gtcagccggt ttacgctcta
    46861 gaaattcggt caaaccaagg atctgcgcag cttccttgac acgacggtca atatcatctt
    46921 ttttgtactt acgtaatttc aaaccaaagg ccatgttatc atacacggtc atgtgtgggt
    46981 aaagggcgta gttttgaaag accatggcaa tatcacggtc tttaggagac ttgtcattca
    47041 ccacttcgcc accgattttt aactctcctt cagaaatgtc ctcaagacct gcaatcatac
    47101 gaagagtagt cgatttacca catcctgaag gtccaacaaa gacgataaat tctttatctt
    47161 tgatgtccaa gtcaaaatct tcaacggcat aatgagtagt atttgggtat tttttataga
    47221 tgtgatttag atttaattca accatgtgta actcctattt agccagcaaa cgttctagct
    47281 gactgcaaag ctagaaaccg ctgatatctt ttatatgatg cttttattat aacaaaaata
    47341 gcgctttcaa aacagagaca aagcgaccaa acttctttgg gcaacttgca caatagcagg
    47401 actagtcaag tcgtaaaggc ccataaaaca agaaaaccat gtaaacacag gccggtcaca
    47461 ggagccaaaa caagctgaac gatctatcat cctccttatc agctacagtc caggcagcaa
    47521 aaaagaaagc cctgtggctt tcctaatatt tcaacaaaaa gagatgagca aaggctaaat
    47581 catccaattg cttcaagtat agtcctgatt gctgagcaaa tttatcaagt ttgtattgca
    47641 aggagttacg gtgaatatac aagcgctgcg ctgtctgcac cagattgccg tgttctgacc
    47701 atagagcgtg aaccacatcg gccatatcac tttgaggact taggaattgg tgaaactgac
    47761 gcgtcaatgc caaaaaagtg tggtgcgaca gcaaagacca cagcatcagt cctgaaaatg
    47821 ttaataattt tctgcctgat tgttgcctta aataggccgt taacagctga cattcctcct
    47881 caaaacactc tctcaatgta cccgcagcta cttgatacca ggcatttcca acaaacatgg
    47941 ttaaggccaa accaaaatca ctttctagcg ttggcaagag atcttcaagc cagcgcaaga
    48001 cctttattga gattgcttgt cggcataaaa atgccgtctg agttgcactg ataggcaaaa
    48061 tgacctcaag ccctgcaatc atctgttgga gcaactcaac taagtcagct ggtaaagcct
    48121 gctgatgatt gaggtaaatg aactgagaag tctcaaacag ctgaggggga ttgccctgat
    48181 gatcaagcaa ataccgctgc caaggtgacc ctagcggctg caagacctca cactgaccca
    48241 gtcccacctc aagtaataag cgctcttttt cggataaaca tgatttggga aaatgcacat
    48301 attggtcccc ttctttgaca ctcacccact ctttatctcc taacggatga gggcctactt
    48361 gcatctctgg aaaatagtct tttagctcca tagccattcc tttcatgacg gtctttaaac
    48421 cattataaca catgactctt tatcacacag ttcggtttgt tatcagcacg attttgtatt
    48481 ttctgacttt ctaatcatta aagctaaata atggttattc atttttagca agaacattca
    48541 atcaaatagc tatttatcgg aatattaatt tatgtttata ctaaaacagg tattatttct
    48601 cttttttcat tatcatgaca ttatcattaa gatatcattt aaaaaacaat tattaggttt
    48661 ttgtttgtat ctataaaatt attatgttat tctaataatg gggattgaaa cttaactttt
    48721 aggaggtttc tatgaaaaat tacttatctt ttgggatgtt tgcactgctg tttgcattaa
    48781 catttggaac agtcaagcct gtccaagcta ttgctggacc tgaatggctg ctagaccgtc
    48841 catctgtcaa caacagccaa ttagttgtta gcgttgctgg tactgttgag gggacgaatc
    48901 aagaaattag ccttaaattt tttgaaattg acctaacatc acaacctgct cagggaggaa
    48961 agacagagca agacttaaat ccaaaatcaa aaccacttgc tacagataaa ggcgcaatgt
    49021 cacataaact tgaaaaagct gacttattaa aggctattca agaacaattg atcgctaacg
    49081 ttcacagtaa cgacggctac tttgaggtca ttgattttgc aagcgatgca actattactg
    49141 atcgaaacgg caaggtctac tttgctgacc gagatgattc ggtaaccttg ccgacccaac
    49201 ctgtccaaga atttttgcta agcgggcatg tgcgcgttag accgtatcaa cctaaagccg
    49261 ttcacaactc agctgaacgc gttaacgtca actatgaagt gagctttgtc tccgaaacag
    49321 gaggtttaga ctttacaccg ttgttaagaa accaatacca tttgaccaca ctggcagttg
    49381 gtgactctct ttcatcacaa gagttagcag cgattgccca atttatccta tcaaaaaaat
    49441 atccagatta tatcattaca aaacgtgact cctcaatcgt cactcatgac aatgacattt
    49501 tccgtacgat tttaccaatg gatcaagagt ttacttacca tatcaaagac cgggaacaag
    49561 cttatgagat caatcctaaa acaggtatta aagaaaagat gaacaacacc gaccttatct
    49621 ctgagaaata ttacgtcctt aaaaaagggg aaaagccgta tgatcccttt gatcgcagtc
    49681 acttgaaact gttcaccatc aattacgttg atgtcaacac caacgaattg ctaaaaagcg
    49741 agcagctctt aacagctagc gaacgtaact tagacttcag agatttatac gatcctcgtg
    49801 ataaggctaa actactctac aacaatctcg atgcttttgg tattatggac tataccttaa
    49861 ctggaaaagt agaggataat cacgatgaca ccaaccgtat cataaccgtt tatataggca
    49921 agcgacccga aggagagaat gctagctatc atttagccta tgataaagat cgttataccg
    49981 aagaagaacg agaagtttac agctacttgc gttatacagg gacacctata cctgataacc
    50041 ctaacgacaa ataaccacga tcttctaaaa cgatgagatt aactgacaaa aaaagcaagc
    50101 aacacgctat caacagttgc ttgctttttt ctaacctctt agttgtagag actagtgaca
    50161 tttcgtgtct aaaataatcg taactggtcc atcattgacg agactaacct gcatatctgc
    50221 cccaaaaacg ccacgctcaa ctggcacaaa ctctgccaat tgttcattaa agctatcata
    50281 caactgacta gccaaatctg gcttagcagc accagtaaag gcaggacggt ttcccttttt
    50341 ggtgtcagca taaagggtaa attgcgacac agataagata ctgcccttga tgtcttggat
    50401 agactgattc atcttgccat cagcatctga aaaaatgcgc atgttgacta tttttcgcac
    50461 agcgtaagcc aaatcttctg cattatcatc tggtcccaca cctactaaca acaatccttg
    50521 attaatagcg ccagcaattt tcccatcaat agaaacagag gcctctttga cccgttgcaa
    50581 gacaagtttc atggttttat cctttcttaa ccattggtcc gcttcacgct ataaacatct
    50641 ggaactgcct tgattttttc gacaacagtg gtcagatgcg tcagatttgg aatgccaaag
    50701 ctcacgtgaa tattagcaaa cttcatgtct ttggtcggct gagcattgac tgtcgatatg
    50761 ctcttggttg agtttgataa aatttggagc acatcattaa gcaggccact acgattgagc
    50821 ccatagatat caatttcagc ctgataatct ttactcgaat tgtccaaatc ccactcgacc
    50881 tcaatcaagc gttcttggta gccatcttga ctcttaatgt tatgacagtc cgatctgtga
    50941 atcgcaatgc cacgcccttt ggtaatgtag ccgtcaatag gatcaccagg tacaggatta
    51001 caacacttgg caatccgcat caagaggcct gatgctcctt ggataatgac tccattttca
    51061 ctgcgaacct tgagcacatc tttgttttcg tgtttgacct caccgccctt aaccaattct
    51121 tcagcttctg ctttagcctt ggccctttct tcttcacggc gctctttttc ggttaacttg
    51181 ttaaaaacac tgataggact aatgtcacca aacccaacgg ctgcatagag cgattcttca
    51241 ctcttcacac tgacttttgg aaggatcgct tcaatgcgtt ttttgtcaag gtatttattg
    51301 gcaacgtagc cctgctcttg aaaataagac accaacaaat cacggccttt attcactgac
    51361 aattccttgt cttgattttt aaagaactga cgaattttgt tgcgagcctt attggttttg
    51421 accagtttta cccagtctcg actagggcca aaggaattgg cattggtgat gatttcaacc
    51481 acatctcctg tttttaactt ggcagtgaga ggaaccatac gcccattgac tttggcacct
    51541 gttgcttttt caccgatttg cgtatggatc gcataagcaa aatcaatagg acctgattct
    51601 tttggcaact cctgaacggc ccctgtcggt gtaaagacat aaatccgttc agaaaaaata
    51661 tcttctttga ccgaatccac aaagtccact gcatcgccat ttgaggcatc ttgcaattct
    51721 accagctctt tgatccagtt catgccaacg gcttgctcag cctgattgac cttaccacgc
    51781 acgccttttt tataagccca gtgtgcagca accccgtact cagccacttg gtgcatttcc
    51841 ttagttctga tttgaatctc aataggtcct tttggcccat acacagtggt atgaatcgac
    51901 tggtagccat tagctttagg agctgcaata taatccttga agcggcctgg catggggcgc
    51961 caaagctcgt gaatatagcc aaccatagca tagacatcgc tttgcgtttc catgacacaa
    52021 cgaatggcaa tcagatcaaa aatctgatcg aaacgttttt tcttgtcccg cattttccga
    52081 taaatcgaat aaatgtgttt tggccggcca tacacatctc caaacaagcc ttgttgtgtc
    52141 gtataggttt tgaccttact gacaatagcc tctaccaaag cttcacgctc gcgacgtttt
    52201 tctttcatca tatgggaaat tttgtaaaat tcggtttcat tgaggtaacg aaaagccaaa
    52261 tcttctagtt cccatttgat gcgactaatt cccaaacgat gcgccaaggg ggcatagatt
    52321 tccatggttt cgcgcgaaat gcgctcttgt ttgtccttgc gcaaatgttt gagggtgcgc
    52381 atattatgca ggcggtcagc caatttcacc aaaatcacgc gaatatcttt ggacatagcc
    52441 atcagcattt tgcgatggtt ttcggcgagt tgctcctcat gagatttgta ctcaacttta
    52501 cctaacttgg tgacaccatc aacgatatca cgagcatctt ggccaaagtc tgcttcgatc
    52561 tcatctaagg taatatccgt atcttctacg acatcatgta aaaagccaca agcaactgtc
    52621 acagcatcca gatgcaaatc agccagaatc cccgccacct gaatcggatg gacgatatag
    52681 ggttcgcctg actttctcac ttggtagaaa tgggccgctg ttgcatatgc taaagccttt
    52741 gccacaaaag ccacatcagc cttggtcata taggtggccg ctaaggcaat gacttcttct
    52801 cctgttacgt tcattatttt tgccatttcg ttcctcatct agcatttctt ttccctattt
    52861 taacacttta cacacaaaag caaaaggcag aatctgctaa gaaagcgctt agtgtgacct
    52921 tttcttagaa aacggttagt gagctgcttt tgatatcttt tgggatctct caggcgtgag
    52981 catgatacca acggccagct cccgaccaaa aatagagaga accaaggggc tctctctatt
    53041 atgtttgctt atcagtaagg tctctcaatt cagaggatta gttgttttct ttgcgttttg
    53101 taacggctag gacaccagct gttgtcatta ctgctactgc tgctgctgta aagaatgggt
    53161 tggcttgctc accagttgct ggtagacgtc tgtggttgtt agctacaggt gtcttagcca
    53221 tattgccatt gcggcgttct ggcgtttggt tagcttcttt gtcagcaggt ttttctggtg
    53281 ttggtgtcac ttctgggctc ttttctggag ct
//
LOCUS       DATURV010000006        49668 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DATURV010000006 DATURV010000000
VERSION     DATURV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49668
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.006"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(74..373)
                     /locus_tag="VCF75_000335"
     CDS             complement(74..373)
                     /locus_tag="VCF75_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545929.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     VNFDDLRLTMVDMVSLSSEYDVIVTDMILEQTTDSEILFFNQMAPSVVANRLTDM"
     gene            complement(529..996)
                     /gene="perR"
                     /locus_tag="VCF75_000336"
     CDS             complement(529..996)
                     /gene="perR"
                     /locus_tag="VCF75_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEP2545930.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICSDCQAKDQPDF"
     gene            complement(1048..1503)
                     /locus_tag="VCF75_000337"
     CDS             complement(1048..1503)
                     /locus_tag="VCF75_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEP2545931.1"
                     /translation="MIYSFQNPSEDVLKAYLESAKTIAIVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEAQTILWSAGKEAIVMNRCLKIDYLQLVAKPECITNH"
     gene            complement(1681..4323)
                     /gene="polA"
                     /locus_tag="VCF75_000338"
     CDS             complement(1681..4323)
                     /gene="polA"
                     /locus_tag="VCF75_000338"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEP2545932.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REDIIGFAWGHGEQIYASTDLGLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKVADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(4454..6181)
                     /locus_tag="VCF75_000339"
     CDS             complement(4454..6181)
                     /locus_tag="VCF75_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEP2545933.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     KSKVSSWTK"
     gene            complement(6197..7393)
                     /locus_tag="VCF75_000340"
     CDS             complement(6197..7393)
                     /locus_tag="VCF75_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEP2545934.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(7881..8972)
                     /gene="ulaG"
                     /locus_tag="VCF75_000341"
     CDS             complement(7881..8972)
                     /gene="ulaG"
                     /locus_tag="VCF75_000341"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEP2545935.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(9225..10871)
                     /locus_tag="VCF75_000342"
     CDS             complement(9225..10871)
                     /locus_tag="VCF75_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEP2545936.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INKALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(11196..11900)
                     /locus_tag="VCF75_000343"
     CDS             complement(11196..11900)
                     /locus_tag="VCF75_000343"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEP2545937.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(11902..12765)
                     /locus_tag="VCF75_000344"
     CDS             complement(11902..12765)
                     /locus_tag="VCF75_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEP2545938.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            complement(12770..13432)
                     /locus_tag="VCF75_000345"
     CDS             complement(12770..13432)
                     /locus_tag="VCF75_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEP2545939.1"
                     /translation="MTHIPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     MKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIRRI
                     WG"
     gene            complement(13523..14008)
                     /locus_tag="VCF75_000346"
     CDS             complement(13523..14008)
                     /locus_tag="VCF75_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2545940.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSTYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
                     A"
     gene            complement(14131..14409)
                     /locus_tag="VCF75_000347"
     CDS             complement(14131..14409)
                     /locus_tag="VCF75_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2545941.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(14480..15913)
                     /locus_tag="VCF75_000348"
     CDS             complement(14480..15913)
                     /locus_tag="VCF75_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP2545942.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYFFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(16220..18721)
                     /locus_tag="VCF75_000349"
     CDS             complement(16220..18721)
                     /locus_tag="VCF75_000349"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEP2545943.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGKEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSGFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(18933..20072)
                     /locus_tag="VCF75_000350"
     CDS             complement(18933..20072)
                     /locus_tag="VCF75_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEP2545944.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELAQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            21018..21185
                     /locus_tag="VCF75_000351"
     CDS             21018..21185
                     /locus_tag="VCF75_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545945.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGSHQTVSANDASYSNGFYGHT
                     GPDSWLLYTVW"
     gene            21595..21831
                     /locus_tag="VCF75_000352"
     CDS             21595..21831
                     /locus_tag="VCF75_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545946.1"
                     /translation="MKKKLSLFMIATAAVVSLSLATPENQTVSANDTQVTCVGCPDCG
                     PKWSCCPLTCMRDLLRGWGKSAAYYCTWGYYYSE"
     gene            complement(22017..22448)
                     /locus_tag="VCF75_000353"
     CDS             complement(22017..22448)
                     /locus_tag="VCF75_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545947.1"
                     /translation="MKQQSYQPLRFVYLLVALFAAMLLIARPVMADGENNDPANAAYY
                     KGKSAGEEAGKTAGKEASWTDLTPTNPTIPEPPSDIRETTNKQHYKDGYTDGYRDGYN
                     EGWKSQHPVLTPVKVIWDLISHWLQQFFPNNQATTTVQSMS"
     gene            complement(22703..24418)
                     /gene="slo"
                     /locus_tag="VCF75_000354"
     CDS             complement(22703..24418)
                     /gene="slo"
                     /locus_tag="VCF75_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEP2545948.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTTEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRS
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            complement(24442..24927)
                     /locus_tag="VCF75_000355"
     CDS             complement(24442..24927)
                     /locus_tag="VCF75_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEP2545949.1"
                     /translation="MYKVPKSLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMVHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            complement(24932..26287)
                     /gene="nga"
                     /locus_tag="VCF75_000356"
     CDS             complement(24932..26287)
                     /gene="nga"
                     /locus_tag="VCF75_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEP2545950.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFVSPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     KYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            complement(26658..27197)
                     /gene="nusG"
                     /locus_tag="VCF75_000357"
     CDS             complement(26658..27197)
                     /gene="nusG"
                     /locus_tag="VCF75_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEP2545951.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            complement(27370..28413)
                     /locus_tag="VCF75_000358"
     CDS             complement(27370..28413)
                     /locus_tag="VCF75_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP2545952.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSSKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIETAK
                     KAIIEGTITVPEN"
     gene            complement(28740..30032)
                     /locus_tag="VCF75_000359"
     CDS             complement(28740..30032)
                     /locus_tag="VCF75_000359"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEP2545953.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGERIDHYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            30470..31348
                     /locus_tag="VCF75_000360"
     CDS             30470..31348
                     /locus_tag="VCF75_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545954.1"
                     /translation="MIKKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLVKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPQIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHDANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            31361..32623
                     /locus_tag="VCF75_000361"
     CDS             31361..32623
                     /locus_tag="VCF75_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEP2545955.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDSM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQSPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTHA"
     gene            complement(32743..33369)
                     /locus_tag="VCF75_000362"
     CDS             complement(32743..33369)
                     /locus_tag="VCF75_000362"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEP2545956.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYAIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(33414..34829)
                     /locus_tag="VCF75_000363"
     CDS             complement(33414..34829)
                     /locus_tag="VCF75_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEP2545957.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(34830..36605)
                     /locus_tag="VCF75_000364"
     CDS             complement(34830..36605)
                     /locus_tag="VCF75_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEP2545958.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     ESNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(36806..37126)
                     /locus_tag="VCF75_000365"
     CDS             complement(36806..37126)
                     /locus_tag="VCF75_000365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEP2545959.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(37123..38121)
                     /locus_tag="VCF75_000366"
     CDS             complement(37123..38121)
                     /locus_tag="VCF75_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEP2545960.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            complement(38137..38721)
                     /locus_tag="VCF75_000367"
     CDS             complement(38137..38721)
                     /locus_tag="VCF75_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEP2545961.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(38790..39269)
                     /locus_tag="VCF75_000368"
     CDS             complement(38790..39269)
                     /locus_tag="VCF75_000368"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEP2545962.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(39271..41271)
                     /locus_tag="VCF75_000369"
     CDS             complement(39271..41271)
                     /locus_tag="VCF75_000369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEP2545963.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEERHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKQHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVAIWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(41261..41581)
                     /locus_tag="VCF75_000370"
     CDS             complement(41261..41581)
                     /locus_tag="VCF75_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2545964.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(42035..43054)
                     /locus_tag="VCF75_000371"
     CDS             complement(42035..43054)
                     /locus_tag="VCF75_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEP2545965.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(43091..43456)
                     /locus_tag="VCF75_000372"
     CDS             complement(43091..43456)
                     /locus_tag="VCF75_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP2545966.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            43630..44310
                     /locus_tag="VCF75_000373"
     CDS             43630..44310
                     /locus_tag="VCF75_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP2545967.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVAKDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(44432..45079)
                     /locus_tag="VCF75_000374"
     CDS             complement(44432..45079)
                     /locus_tag="VCF75_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEP2545968.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(45082..45741)
                     /locus_tag="VCF75_000375"
     CDS             complement(45082..45741)
                     /locus_tag="VCF75_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEP2545969.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVALIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(45753..46937)
                     /locus_tag="VCF75_000376"
     CDS             complement(45753..46937)
                     /locus_tag="VCF75_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP2545970.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLIDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            47058..47969
                     /locus_tag="VCF75_000377"
     CDS             47058..47969
                     /locus_tag="VCF75_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP2545971.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEVKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFMNQRF"
     gene            complement(48038..49444)
                     /locus_tag="VCF75_000378"
     CDS             complement(48038..49444)
                     /locus_tag="VCF75_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEP2545972.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSLVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFLVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT        14887 a        10753 c         9036 g        14992 t
ORIGIN      
        1 taacttattc ttaatcttat ttatcgctca ttttttaaat tccgatgctc tcacagatat
       61 tatcattgtt tagttacatg tctgtcaatc tattcgccac cacactaggg gccatctgat
      121 tgaaaaataa aatttcggag tccgttgttt gctcaagaat catatctgtc acaattacat
      181 catactctga agacagtgac accatatcaa ccatggtcag cctcaagtca tcaaagttga
      241 caatctcaaa aaatcctcca aaatactctt ttaagaagtt gccaacattg ttataacttc
      301 tctcaaccac taatagtttc aagcgacgac gcgattgatt tgtaagaaag agattttccc
      361 aggtgaggaa caaacaatag aggagatgat tgatggttct ttcctttaat tccttatgat
      421 ccccagcaaa taactcttta acataagcaa caaaggtctg gtagtgaact gggggttttt
      481 gatttgtaaa ggcattatat ttataatgcc tttatttaag ggttaactct aaaaatcagg
      541 ttggtctttt gcttggcaat ccgaacaaat cccgtaagca ataactggga tacgcgtgac
      601 cttataacct gtttgctcat gggcttcttt ggcaatatcc ataacatcaa catccataaa
      661 gtcagcaatc ttcccacata tttcacaaac cacattgacg tgttgatggc ccataaagtc
      721 atagtaggtg gttagatcat tgctgatttt caattctgag acaaaacctt catcaactaa
      781 aactttcaaa ttattataga ctgtggcaag gctcatgtta ggaaagttag gctgtaagtc
      841 acggtaaatc ttatcggcac tagggtgttc tgtggactga atcatgtaag aaataatggc
      901 tttacgtgtc tctgtaattc gaatatgttt ttcccgtaga tgctctagga cattttcata
      961 ggcatctagg gcttgctgat gtgaatgaat gtccataagc tgctactcct atcctttttt
     1021 cctttattat cacgcacgtc tctagtttca atgatttgtg atgcattctg gtttagcgac
     1081 taattgcaaa tagtctattt ttaggcagcg gttcatcaca atagcctctt tgcctgccga
     1141 ccataaaatg gtttgtgctt cttggttttc aaggcctaat tgcgcccaaa agactttggc
     1201 ctgacctgct aaaaagtctc tggcaacttc tggtaaaaac tcacttctgc gaaagacatc
     1261 tacaatgtca acctcaaagg gaattgcctt gatactggca tagaccttct ctcccaaaat
     1321 aagctgacct gctaatttgg gattgactgg aatgatccga taatccatag cctgcataaa
     1381 tttggcgaca ccataagctg ctgtgtcttt gcggtcagaa agaccaacaa tcgcaatggt
     1441 tttggctgac tccaagtaag cttttaggac atcctctgag ggattttgaa acgaataaat
     1501 cacttgttcc cctatctatt ctaaagctgt tcaatatagg aggataactc ctaaagcatg
     1561 gcttcaatca cttttaacct tatccgtcaa aaagctcaaa gcagcttcat gacttctcct
     1621 acaatttttt agtttattat aacataagtt gtcaaaatga gaaataacaa gtaagaaaac
     1681 ttatttggct tcgtaccagc tctgcccagt actttcatcg acacataaag gaacagctaa
     1741 atcaacagca gcttccatgg tgtctttgac aagttttttt atagcagtca actcatcatt
     1801 tggcacttcc aaaacgattt catcatgcac ttgaagcagc attttagctc taaaaccacc
     1861 tgcttgcaag gccttatcca gattaatcat agcgattttg agaatatcag ctgcgcttcc
     1921 ttgaatgggc gagttgatgg ctgtacgctc cgcaaaagag cgaacgttga aattacgaga
     1981 gttgatatcc ggtaattcac gacgacgctt aaagagggtc tctacatagc ccttgtcttt
     2041 agcctcacgc accacatttt ccatataggc tttaatccca ggatagcgtt caaaataggt
     2101 atcaatgtaa gacttggcct gtttacgggt gataccaagg ttatttgaca aaccaaaatc
     2161 agaaatccca tacacaattc caaagttaac agccttggca ttacggcggt cattggcagt
     2221 aacatctgct accttgtcaa tcccaaacac acgcatagcc gttgaggtat gaatgtctgc
     2281 cccttcatta aaggcagcaa tcaaatgctc atcccctgaa atatgtgcca gtacccgcaa
     2341 ttcaatctgt gagtaatctg aactcaacaa gacggcatct tcatgagatg gggtaaaggc
     2401 cttacgaatc aaacgcccct gttccaaacg aatgggaatg ttttgaagat tagggtccac
     2461 actagacaaa cgccctgtct gggtgagatc ctgcacatag cgggtatgaa tcttgccatc
     2521 agccagaata taatcctgta aaccaatcac ataggtggat tgcaatttag taatctggcg
     2581 gtaatccaaa attttagcca caattggagc aatcggtgcc aaacgttcca aaacatcaac
     2641 agctgtagaa taacctgttt tggtcttctt agtcatctca agaggtagtt gcatcttctc
     2701 aaacaagata ctgcccagct gtttaggcga attaatgtta aattcttggc cagccatatc
     2761 ataaatctct tgggtgagcg cttcaataat gaccttgttt tgctctgcca tatcttgcaa
     2821 agtcgcccta ttaaccttga tcccttcaat ttccatttta gcaaggacat tagccaaagg
     2881 caattcaata ttctgataga ggtctagttg ctcatgggca gtgagcttgt ctagcatttg
     2941 agacttgcta tctaataata ctttgacctt acgagctaag tgttctagta agacgtcttt
     3001 atcaggaaca gcccgtttag cacctttacc atagaccgta tcgtcttctt ccaaagagat
     3061 gtcagtaaaa atgcgggcaa tggtgctaag ctcattgtct tctacagttg acagaaggta
     3121 attagctaat ctagcatcat agctaggtgc taccaactca attcctaagt ggctcaacaa
     3181 gaccttgcta cgtttaaaat cataagtggc aatgggtttt tggaagacct gcttaaaact
     3241 atccgtcgct aataggccaa ggtcagtgga tgcgtaaatc tgctcaccat gtccccaagc
     3301 aaaaccaatg atgtcctcac gatggtaatt atcccgaagc gtttcaaagt agaagatgtc
     3361 ttctgcgcta aacaaatcag cagacacatt ggtcacttct tgataggaaa tggcagctac
     3421 tggctcttga ggcatctggc tagcaagacc tttcttgagt tgcacaaaat ccatctcatc
     3481 gtaaaattga gagaggctag caacatctgg accattgtag acaatatcat ctaagccaat
     3541 tgtgatagga gatgctgtat tgatagtggc gagcgtttta gataggaaag cttggtcacg
     3601 gtcattgatc aagttttctt tcatttttga ggccttgaat ccatcaatat gctcgtaaat
     3661 gccttccaaa ctaccaaatt catgaaggag cttaaggcct gttttttctc caatttttgt
     3721 cactccagga atgttatccg atttatctcc catcaaggct ttgaggtcaa taaactggtt
     3781 tggcgtcaag cccatttttt ccatgaggta agccggtgtg aattcttcaa attcggcaac
     3841 ccctttttta gaaatttcaa ccacggtatt ttcgtcagtt aactggatca aatctttatc
     3901 accactgaca atggtcacat caaaaggcac ttctgtgcgc tccgccattt tatcaagcgt
     3961 cccgataata tcgtctgctt cgtagtgttc caactcataa taagcaatcc caagggctgt
     4021 caacatctca cgaatataag gaaactgctc acgaaattcc tctggtgtct tggcgcgacc
     4081 cgccttataa tcggcataca tttctgttcg gaaggtggtt ttgcccgcat caaaagccac
     4141 cagcacgtga gtaggctgga ctcttttcat catgtggtct agcatcaaat gaaagccata
     4201 aatggcattg gtatgaagac cactatgatt tttaaagcgg tcaatttgat tgtagagggc
     4261 aaaaaaggca cgaaaggcaa cagatgagcc atcaattaat agtaatttat tcttgttttc
     4321 catagcctta ttataacaca gagggacggg cttttctcct ttaagccatt ggttttgctg
     4381 ataagtaaat agttaaccta aaaagcctag gaaaattcct agacttctct atttgctatt
     4441 tagacaaaag cacttatttt gtccaacttg atactttaga tttgttagcc tctacccatt
     4501 ttttggcggc agcttctggg ctcatgcctt tattgatgtc aagcatgact gcttccatat
     4561 ctttttgggt ccagtggaac ttatcaatga ttttgtagac atttggaaga tcttttttca
     4621 agcctttacg agcaatggta ttgatgttct cagtcgagcc aaaggtttct ttaggatctt
     4681 taaggtactt caagtcatac ttggcaaaca tccagtgtgg tgaccaagct gtcacgacaa
     4741 taggatcttt tttcttgatc gcttgatcca aggaagtcgt catggctcct gttgatgccg
     4801 ccactaactc ccaactagat agattatgat actcttttag agtctttttg gctgctgcca
     4861 tgatacctgc acctggttcg atccctgtga ttttttgatc ggcttgtttg cttaagtcct
     4921 caatactatt gacatctgtc atgtattttg gaacagctaa accaagtttg gtccctttta
     4981 ggttaggtcc taaatcatct aattttgact tgtatttctg atattgttgg ccatgggtga
     5041 ctggcaacca agcactagtt gaaaaatcag cgttcccatt tgcaacggtt tgccacataa
     5101 cggcattgtc aagcggcgtc aaggtcacat ggtaaccttc gtttttcaaa acttccgcaa
     5161 tcacatgagt tgaggcaacc tcagaatccc actgcacgta agcgatgtta acagtttcac
     5221 ccttatctgc cataccagaa gtcatagcca taataccgcg tcctaaagca gctatcaaaa
     5281 agacagcaag ggctgctaga ccaatccatt tattggtttt gccagctttg gctttttctt
     5341 gaggtttgct attgaacaac tgtgtcatac ggtcaagcac aatcgccaag ataaccaagg
     5401 caagaccact gacaaatcca ctaccaatat cagcgtgttg aagggctgac aagacttcac
     5461 gtcctaaacc aggagcacca atcatggagc ctgttactac catggaaagg gctaacatca
     5521 tggtctgatt aacacccgcc atgatggtat ttttagctaa aggcaattct accttgaaga
     5581 gcttttgttt cccagttgac ccaaaggcat ctgaggcttc aatcaattct gttggaatgt
     5641 cacgaatggc caaatttgta aaacgaactg ttgggggcaa ggcaaagatg actgaagcaa
     5701 atactccagg caccatacca ataccaaaaa aggcaacggc aggaatcaag taaacaaaag
     5761 ccggcatggt ttgcataaag tccaaaatag gattcacaat ctgacggaca gtggcatttt
     5821 tagccatcca aatccccagc ggaataccaa taagtaccga aatcaaacta gctactaaca
     5881 ccagagtaaa ggtattcatt agctgcttcc ataacccttg attgtaaata aagagcaatc
     5941 ctaacaaggt aaaggttggc aaaggccatt ttttcttagc aaggaaaaac atccctgctg
     6001 ttactagaac aataaaaagt aacgggtgga taaataaaag tgttttggtc atccaatcca
     6061 tcaaaaagct accaactact tgcataatat caaatagacc tgagaaagtt ttagttagcc
     6121 actccgttag ctgctccacc agttgagcta ctggtaattt tgtttgtaat atcgtttcca
     6181 aaatgcatca ctccttttag ttaaggtctt cgtctgagat attagccaag gcttcaatca
     6241 ctcgtcctcg aataatgaca cctaacagac gattgttatc atctgtcact gcaatcggag
     6301 cagatgaatc atagataaga ggcaaaatat ctgtaataat agtatctttt gagacagttc
     6361 tcacatcgcg atcaatcact tctgatagcg gtaacccttt tttgcgcgcc tctatagcgg
     6421 catcggccgt caaactaccg actaattggc ggcggcgatt cgtcgccatc aacatagaca
     6481 cctcttcgtt gtgcatacgg ttcaaggcaa cttgaggtcc atctaattca acagtagttg
     6541 ttaacggttt gatcatgata ttttgtgctg tcaagacttt agaacggtcc acatcctcaa
     6601 cgaattcacg cacaaagtca ttggctgggt tagtcaagat ttcctcacca gtaccaattt
     6661 gcataatttg tccgtctttc atcaaagcaa tccgatcacc aatccgcaag gcttcattca
     6721 agtcgtgact gataaagatg atggtttgtt tcatgctatc ttgcaaatca agtaattcat
     6781 cttgcatctc acgacgaatc aaaggatcaa gcgctgagaa tgcctcgtcc attaagagaa
     6841 ttttagggct attagctagc gcacgggcta ggccgacacg ctgctgcatc ccaccagata
     6901 gttggtttgg gtactggtct ttaaaatcta ataggcctga attatcaagg gctttttctg
     6961 ccaatcgctg gcgttcttct ttgggaacac cacgtaattc caaaccaaat tcggtatttt
     7021 ccaaaatggt tttatgagga aagagggcaa agctttgaaa aaccatgtta atgtcatggc
     7081 ggcgcacctc acgcagctga tctgctgaca tggttgagat gtctttacct tccagcaaga
     7141 tagatcctgc tgaaggttca atcaaacgat ttagcatgcg gacaagggtt gatttcccac
     7201 ttcctgatag ccccataata acaaagattt cacctttttt gacctcaaaa ctagcgtcat
     7261 agacacctac agtagcgcct gttttcttaa aaatctcact cttatttttg ccagtcttta
     7321 ccatctcaag agctgctttt tgttttttgc caaaaatttt actgagatgt ttgacttcta
     7381 aaattgtttc cattcgtttt ttctcctagg taatcgttta tcatgtgaaa tagtgacata
     7441 gctttggcga aatattagct aatatttaac aaagtagctc tacacaatta ataaacgtgt
     7501 ggtcactttt ttacttaaaa aaagaatctg ccgaaacaga acataactat attatcacgt
     7561 gtggtcactt tttgtcaagg acaataactc gatctggtaa tgccacaatg ataggtgatc
     7621 gctttcattc ttttagatat ggtcttttaa ccagtacaat tttttatcac aactgatgac
     7681 tatttctttg atgattaggc tattgttaca ttagaaatcc ttcttttacc tataatatct
     7741 attttgcggc catcgtaaat aacgaccacg gtgactctta aacatttgct catctctcca
     7801 aaaaaactca cctaagacag tgtcctatga agcgagttag cttaatcagg cactatcatc
     7861 ttaggtgagt agggtgttgc ttatagtaaa gctttaaatt ggatattaga atcttctaag
     7921 aagcagtcat caaatccacg aggatgatgg tattcgatac gattttggtc ttgtggatag
     7981 gtgtacttgc caccaacctc ccagataaag ggatggaaat cgtattggag acgttctttg
     8041 cgcattttcc acagttcaag gatttcatct gttgatgcca taaagtttga ccaaatgtca
     8101 taatgaactg ggataacgac tttagcacgg agattttctg ccatacgaag taggtcaaca
     8161 gaagtcatct tgtcttgaat cccaattggg ttttccccat agttatttaa cacaacgtca
     8221 atatcataat cacgtccatg tttagcaaag tagttcgaaa aatgagaatc cgcaccgtga
     8281 tagatggtac cacctggtgt ttcaaaaata tagttaacag ctttgcgagc catgtcatca
     8341 tctgtgatag ctaaacctgc taaatcacca tcttgagcat cagctccttg tattgggaga
     8401 gttaccagac aggtgcggtc aaacgactct acagcggtga tcttaatatc tttgaattca
     8461 aagctatcac ctggctttaa gatcataata cgatcttttg gcacacccca atttttccaa
     8521 acttcaccac attcataagg tcctacaaat ttaacatgat taagttttgg gttattgata
     8581 atggctgccg cagtattgat atcgatgtgg tcactatgat aatgagaaac caaataataa
     8641 tccaactcat taatcataaa tggatcaatc accattggtt gggccctaag attgggctgt
     8701 aatttacgag ctcctgccat attagccatt tgatgtccac gaaccatatc cttaacttgc
     8761 ttggtcgctt tcccacgatt tgaccataag tccatgacaa cattagcacc gcctggtgtc
     8821 ttaatccaga taccacagtt tcctaaccac cacatcgcaa agttgtcagc tggcacgact
     8881 tcttgctcga tttcttcatt taaccatgtt ccccattctg gaaacgtgtt aaggatccat
     8941 gattcacgtg taatatcttg tactttagtc attagggttt cctccgaatt tgttcttcgt
     9001 ttctatactt taagtataga ctttcttgtc aatgataaaa agaccaagtg tcacacagat
     9061 ctgtgttcaa aagtggactg actttttcat tagctcagtc taatactatc tcattttgca
     9121 cactgtatct cgcatcaata ctgtattcaa agctatatta aaaacctcag ttatattttt
     9181 ctatgtcata gaaaactgag gtactttgac gacttaacac ttactcatag cagctcgcga
     9241 tgaatgagtt tttcaatttt agaaaccaag gcataacgat ctgattcatc ttttacaaca
     9301 gcttcaatag cttttgtgag ctctgtagaa aagcgggagt gctcactcaa gcgtccttgc
     9361 tttgaaaagc gtatcaattt gatgatatca tcgtcactta aaatagggtt aacaataagc
     9421 atcgggattt tagtctttag cgtatctgta gtagcaacaa tcatatcgac tgctaaaagg
     9481 tcgtaaacac tctgatactg ttctgtcgta aaaacagctt caatctgacc atttgctaga
     9541 tacctttgac actgtttgaa caataacttt tgaatgccta tcccatcatc tgaaacaata
     9601 acgagtttgg tcttttcttg ctctgtatta ttgtgacgca gctctccccc tagatggata
     9661 gtcaagtagg caatatcatc gtctgtcaag ctaatcgtcc aatcttgttc caaaatagtg
     9721 gcacaagatt gagtcatagc aaacaattct tcgtattttt ctttaatatg gtctgttaag
     9781 ggattaacta aaaaaatccc ataagcttta cggtaaacga gcgccttaca gtgggtcgtc
     9841 aactgcttca gcaaatcctg tttatgcgtg aaatgtaact gataacggct ctccaactgg
     9901 tcaataaagt gactaatggt tgctcgcatg tcatcataat cttgactttc cacgtgatga
     9961 tcttgatctt ttcgaaaaga caacatcaac atcgctacca tactaacttc gatatcatcc
    10021 aaatgaagct taaaattatg gtaaagctct ctagctaagt cctgagcaat gtgatattct
    10081 ttacgtttcc aaatcaaatg aaattcttgt ttaagagcag actttgtttc tgaatcaaga
    10141 cgcatattac gataacttaa aagcatgaaa ggtaaaatct gcaacatgaa ttggctatct
    10201 tgggtattaa ttgtctttcc taagctagct tgagataaaa agagatactc atgaaaataa
    10261 gtcaaaatat ctttagaaaa atagaccgat aagccttgta tctctgagag tttacggttg
    10321 aatatatcaa taaagctagt attgccacct tggtaaacgt ccactaacaa tttgtacagg
    10381 tattgaataa gagccattgg atgacaacca aaataatatc ccctagcctt tgtcgcatga
    10441 agctgaatct tgtattgttt gtcttccagt tcttccctta actcagtcag gtcatttaaa
    10501 atcgtgttac gagacacatc attgatctgc atcagtttat caatggtcac gcgctcagtg
    10561 gaaaccgcaa tataaatcga ggatagtcgt ctgcgctcat cacttttcat gacataatta
    10621 taatcagtca cttcttctaa taataagcga caagcagctt tttgatccgc cgttaaaaga
    10681 atgccaagtc taggataaga gataatctga tctactccct ttggcaaagc cttattaatt
    10741 ttgtcaagct ggtaataaac tttacgacgt gactgattta aagcatgaga aatggccata
    10801 actgtctcag gtgtttctag tttaagtaaa taagacagca agtcataact ctttttatct
    10861 aaaatcatca tgtttacaaa cctcctctct gaaaatccat ccttgcttat tttatcatga
    10921 tttcagtgac tgcgcagcca agttttgaaa aactcaccat aataagattc gcttaatatg
    10981 attcgagcta atcacctcaa ctgatccaac accaacacct tatatctggt ctctaagaac
    11041 tccacaacat acgattcatc cagtttgtgc tcattggcgt ttaaacagtt catcgaaaag
    11101 gacaagtcaa aaggacttgc ccaaacaatg attaacaaaa ccttgaactt tttggcttct
    11161 agtgagaacc tgtgctaata agccatatcg cgacctcatt tttgaccgta ataagcattt
    11221 gggccatgtt tacgcaaata atgcttatcc ataatgtatc ttggcgctgg ctctacgcgt
    11281 ggattgattt gttctgtcaa gcgattcatc ttagccactt cttctaagac aaccgagtgg
    11341 taaacggctt gctctggggt tttcccccac gtaaatgggc catgattacg caccacgatt
    11401 cctggaaccg ccatcgggtc aagacctcgc ttgctaaact cttctaaaat cacgttgcca
    11461 gtttcttgct cgtaagcccc atcaacttct gcttttgtga gagaacgagc acacggaact
    11521 ggtccataga agtagtcagc atgcgtagtc ccataaaacg gaatatcacg acctgcctga
    11581 gcccaaccga cagcttctgt ggagtgagtg tggacaatac cgcccacttc tggccaagct
    11641 ttataaagtt ccacatgggt tggcagatct gaagaagggt tcaaatcacc ttcgaccaca
    11701 ttcccatcaa ggtctgtaac aaccatattt tctggtgtca agaggtcgta gtccacccca
    11761 gaaggcttga tgacaatacg tcctaattca cgacaaactt cagagacatt gccccacgta
    11821 aatttgacaa gaccgtgttg tggtagagac ttgttagccg cacagacacg ttcacgcatt
    11881 tcttgcaagt ttttcgccat attatgccaa ccccgctttc tcaataagtg ggtaaaggaa
    11941 gtcctgagct tctttgatag cagcttttgt ttcctccacc gtgtcacaat tttctgacca
    12001 catttcaatc aaaaaaggcc cattgtagtt ggttttcttg aggaccgcaa acagctcttg
    12061 ccagtccaca caaccttgac caaatggcac atcacgaaac tgcccttttg aggtttccgt
    12121 gacagcataa gtatctttca agtggagggc tgcaatggat ttgtgcccat tgtaaaattc
    12181 actccacaaa tcattatgcc aagcagaaac attgcctgca tcaggataaa caaagagata
    12241 aggcgagtca atctcttttt caactgccaa gtatttttca atagagttga taaaagggtc
    12301 atccataatc tcaatagaaa gcatcacttg ggcttcttcg gcccagtcac aagattgtct
    12361 caagtttttg ataaaacgag cacgggtttc aggcgatttt tcttcatagt aaacgtcata
    12421 accagctaat tgaatggtac gaacgcctaa gtcctgtgcc aattcaatac attgtttcat
    12481 cagtttaagg gacttggctt caatagcagg atcatttgag cctagtgggt aacgtctatg
    12541 gccagaaaaa caaatggtcg ggatgcgaat gcctgtttca taaatggctt tcactaaatc
    12601 aagccgttct tccttagtcc actctaagcg cgctaagcga gcatctgatt catcaacaga
    12661 catttccaca aaatcaaaac ctaagtcttt tgcaaattgc aagcgttcac gccaggtaaa
    12721 ctgttttggc gttgcttttt cataaatacc aataggacgc gccataaagt tacccccaga
    12781 tacgtctgat ttcatctttg aaggcacgcg ccgctgcagc aggatcttct gcttcagtaa
    12841 tgcctcgccc tgcgataaag gtaaagacat caaccccttc aaaaagtttc aaagtgtcta
    12901 catcaagccc accagttact gacacgcgga agcccatgtc aatcaatgtt ttcaccttgt
    12961 taaggtcttt ttcaccccaa gtttctccag caagaagggc atcacgagat tggtggtaaa
    13021 tcgcttggga aatcccagcg tcaagccata attgagcttg ctcataagtc caatcaccat
    13081 aaagttcgat ttgaatttcc ccacggtcac cgcgctcttc tttcatagcc ttgagggctg
    13141 cttccatggt tggaatcgtc gcacagcaaa tgcaggtcat ccagtcagca ccacgttttg
    13201 cattgttttt agcgaccgta ccgccagcgt cagcacattt ggtatctgct acgataattt
    13261 tctctgggaa gaggctgcgt aatacctcaa ctagttcgct tcccacttga agcaaacaaa
    13321 cggtaccagc ttcaatcaca tctacttcgt gacccacagc aacggctgct ttaacagccc
    13381 cctgtaagtc agaatggtct aaagcaactt gtaaatttgg aatatgtgtc atctcttttt
    13441 cctaaaactc ttagccaccg tgcaaactgg ttaagagttc ttatcctttc catcattcgt
    13501 tagtatcctg tgtaaggaaa gcttaagcat tcaagtccat accttctaag taaggactat
    13561 ctttagattc ttctaccatt tctaggacct ctttaggact ttggcaggcc accaagcgtt
    13621 caatggcgtt atctagttca aacaaggcca caatttgtgg gatagccact gttgtgtgaa
    13681 tagaagggtc tgtagcggcc aacgtcaaaa gaacagaaac ctctttacca tctgagaagg
    13741 taactggttt tgttagtgta atcaaagcaa atgcattgcg attaacgcct aaacctgctt
    13801 ctgcatgggg catagccata cctggcatca acacataata agggccgtat ttttctgttg
    13861 atgcaatgat ggcgtcgtag taagttgacg tcactgcttt gctatcaatc aaaggttgaa
    13921 cagctaatcg aacagcctct tgccaagtat cagcacttag ccccaatcgg atggagttgt
    13981 tgtcaataaa ggcttgtttt aaattcataa aaactatctc tttctagtgt cttgctttgc
    14041 aacacacacc atttttacta gttttatcct actaaagaga aagagctgaa aaccttcgtc
    14101 acaactcctt cgttttgatt cagaattagc ttatagcact tcttgtagtt tggttttaat
    14161 ttcattatca tccatcaggt tatcaagacc aaccaagtgg cctttggtac gaccatcaag
    14221 ctcatgaatc aagtggtttg atgccactac aatatcataa ccagaagcta gacctttagc
    14281 ttcaccaact gagcaagaag ctgactgaat atctgtcacg ccaagttgac gaagcgcatt
    14341 ttcaactttc attttgatca ccatagatga ccccatacca ttgccacatg ctgttaagac
    14401 tttaaccatt gttttttcct ccgaaaatgt tttattatta ttttgttaaa atagcagagc
    14461 tatttcttaa gatgcagtgt tattgagctt ccccacggta gtaagcttct ttgtccttag
    14521 cttttgcgaa ctgcaattgt ggaatggcaa gtaagaagat gcaaacaagc acataccctg
    14581 caataccaag gaatttgaag aagtacccaa atggtaacca tggaagaacc aagtcgatat
    14641 taccgtgata accacctgta agtcctaaaa gacctacagc aacagcacca agtgccacct
    14701 gaataatacc tgagataaat gatagagcta ccgctgcttt ccagccacca cgtttatctg
    14761 cataaaccgc aattgccgca ttatcaaaga atacgggaac gaatcctgtg ataattaaga
    14821 ttggattttt aaagataaca agaagagcaa tggtgattaa ttgaccaatc aaaccaaagg
    14881 caaatcctga aagcaccgca tttgaagaac caaagccata ggaagctgca acgtcaaccg
    14941 ctgggaatga ccctggaagc aatttacttg agatcccttg aaaggcattg gtcaattcag
    15001 aaacaaacat acgaacaccc tgcatcaaga taaagagata aactgagaag gtgagagagg
    15061 tttgcaaaat atacatgaag aaagcttgct tagctgggtt gaatgctcca ggtccgatta
    15121 agtccacatc agacataatg tctggaccaa ggacagccaa gatagctccg aaaaagacca
    15181 acatcaaagt tgctgacgca acaacagtat catggaaaat gtttaagaag gttggtaatt
    15241 ttaggttatc aaggttttct tcttttttac caaagaacgg tgcaacctta tccacaaacc
    15301 aaatcgcaaa ttgttgttgg tgaccaatcg caaacccacc gccacctgta aggcgttgag
    15361 ttgcttcaac ggtcatgttt gagctgatag cccagtaaag accacagatg ataccaactg
    15421 cccatgcccc aaaagcattt tggaattgag ggattaaaag aaggacaaag accgaaatcg
    15481 tcgctgcctg ttggaccatg atgtgacctg tgatgaaaag ggtacgaact ttagtgacct
    15541 tgcgaagtgc aaccaaaaga atgttgaccc caaatccaat caagagagct gtcgtagcaa
    15601 cactgatgaa gcccatctct tctaacttag tattagcagc agccaaacca aaataaggat
    15661 caataacggc ggctttcaat tcaaatttct tggcaagagc aaccaagatc ggacgaaaag
    15721 tcgttacaag accgcctgct ccaacgttca aaatcaagta accaacggta gcctttacaa
    15781 aaccagcaaa gacctcataa atgggttttt taaggagtaa atacccgatt aaaaccaaaa
    15841 gacctacaaa gaacgctggg ttttgcaaaa tattttgcga gaaccagttt aggggagcta
    15901 ggagcatttc cataagaact acctcttttc aaagttaata aagataaagt gtttatatgt
    15961 tcattatagg taatgaaagc gttttaaaaa agaccaacta tcacacaaag aagtgttcaa
    16021 tattagactt aagttaactt attttaaaat ttaactacac ttagtaaaaa ggagaaaatc
    16081 accctaaaca acgattaaaa accacaagaa ccttcgttct tgtggttttt ctagatgagt
    16141 tgctttttga atagatcagc tctttggcat taactgtgac gtgacgcaaa taaccactga
    16201 agggctgtgt cacctcaaac tatttgataa caatattaac gagtttgttt ggtactgcga
    16261 tgactttgat gatgtctttg cctgcgattt cggcttggac tttttcgtta gcaagagcaa
    16321 cttcttgcaa ttcttcgcgt gaaaggtctt tagcaactac aagtttagct ttgaccttac
    16381 ctttgatttg gacaacgatt tccacatcgt tttcaaccaa tttgctttca tcataacttg
    16441 gccatggcac gtaagagatt gactcacctg aggctgtcaa ggcttgccag agttcctcac
    16501 ccaagtgtgg cgcaaatggt gcaatcaatt gcacaaagcc tttggcatag tcgctaaaga
    16561 gtttgtcttc cttgttagct gcattgacaa agaccatgag ttgagcgata gctgtgttga
    16621 atttcatttg gtcaacttgc tcagtaacgg ccttaacggt ttcgttgtaa accttgtcaa
    16681 gggctccgct attttcctca gtgatctctt tagttgtgat caaacggtaa acacggtcca
    16741 agaacttacg tgagccttca agaccttctt cagaccaagc gatggaagcg tcaagtggtc
    16801 ccatgaacat ctcgtaaaca cgaagagtgt cagcaccgta ctgctcaaca acatcatctg
    16861 ggttgacaac gttcttaagt gatttagaca tcttagctgg tgcttgttca agctcttccc
    16921 cagtttccac atggaagaag ctaccatcac gtttttcaac cttgtcagtt gccacaagag
    16981 caccacggct atcgcggtag cttgtcccca aaatcatccc ttggttaaag agtttttgga
    17041 atggctcttt ggtaggtaca acaccgagat cgtaaagaac cttgtgccag aagcgagcgt
    17101 agagaaggtg aagcacggca tgctctgcac caccgacata gatatcaact ggcaaccatt
    17161 gtttgaggag ttcctcatca gccagttttt cggtgttgtg tggatcgatg taacgaagat
    17221 aataccagct tgaacctgcc cattgtggca tagtgttagt ctcacgacga cctttcacac
    17281 catcttcacg agtcacttcc aaccagtctg tcacgttagc aagtgggctc tcaccagtac
    17341 ctgaaggacg gatatccttc gttactggaa gaaccagtgg caactcactc tcaggaaccg
    17401 ctgttgaggt cccatcttcc cagtggatga ttgggattgg ctcaccccaa taacgttgac
    17461 ggctaaagag ccagtcacga agacggtaag tgactttctc attaccgacg ccttctgctt
    17521 caagccattc gaccattttg gcaatagctg acgctttgtc aagaccatct aggaagcctg
    17581 agttgatgtg aagaccgtct tctgtaaagg cagcctcttc aacattacca ccttcaagaa
    17641 cagggatgat gtctaggtta aattgttttg cgaattccca gtcacgttca tcgtgggctg
    17701 gaacagccat gatagcacca gtaccgtagc tagcaaggac atagtccgca atccaaactg
    17761 gcatttcttt gccgttgacc gggttaatag cgtagctacc tgtccagaca ccagtttttt
    17821 ctttggcaag gtctgtacga gcaaggtctg atttgagact tgcttgacgt ttgtagtcag
    17881 ccacggcttc tgcctggtca gctgttgtaa tagcatcaac caaggcatgc tcaggagcaa
    17941 gaaccgcgta agttgcacca aaaagggtgt caggacgtgt tgtaaagaca gtgaagtcct
    18001 tatctgtatc cttaaccttg aaggttacat tagcaccagt tgatttacca atccagttgc
    18061 gttgcatgtc cttgattgat tctggccagt caacttcttc caagtcttct agcaagcgct
    18121 ctgcataggc agtgattttg agcatccatt ggcgcatggg ttttctgaca actgggtagc
    18181 cgccacgttc tgatgtccca tctggaagaa cctcttcgtt ggcgatagct gttcccaatt
    18241 cttcaaccca gttaacggga acttcagctt cgtaggctag gcctttttca taaagcttgg
    18301 taaagatcca ctgcgtccac ttgtagtagt tagggtcagt tgtgttaact tcacgatccc
    18361 agtcgtatga gaaaccaaga gcattaatct ggcgtttgaa gttagcgatg ttttcagctg
    18421 taaactcggc tgggtcatta ccagtatcca tagcgtactg ctcagcagga agtccaaagg
    18481 catcccaacc cattgggtgg aggacattgt gaccttgggc acgtttgaaa cggctgagaa
    18541 tatcagttgc tgtatagcct tctgggtgac caacgtggag accagctcct gatgggtatg
    18601 ggaacatatc tagggcataa aacttaggtt ttgatgcgtc tgttcctgtt ttaaaggtgt
    18661 gattatctgc ccaaaaggcc tgccatttgg gttcaatagc ggtgtgatcg taaaatgtca
    18721 tgtgaaatct cccatgtaaa aaatctagtc ttcttattat agcatgagat gaagctttta
    18781 ggaatggtca agacatgact agcaccttgg cttttgagac ttcagacgat tagctatttt
    18841 taaaacctgc tttagcatca accatcctca caaaaagcta ataaaaaaag ccagacttgc
    18901 tctgtaaaca gagccaatct ggacaaatag acttaaaaag caagtgcttg ctcaaaatcg
    18961 gccaataagt cttcgatatt ttcaacacca caagacaagc gcagcaaacc atcaacaacc
    19021 ccttgttcca accgcgcttg tgatgataaa caagcatgag acatcgttgc tggatgtgac
    19081 aaaatggact ctactccacc tagactaaca gctaaaatcg gcaactgaat acgatgggta
    19141 aaggtcatca gctcctcctt agaagccaac tcaaaggata aaacagcacc accattctta
    19201 gcttgttgca ggtgggtatc gtgattagga tgactgtcta agccagggta atgtacctta
    19261 agaactttgg ggtgcttcgc taaatagtta gcaagctgct gagcattttt taccgcttgt
    19321 tccatacgga tccccatagt tttcatgcca cgaagaatca gccaagcgtc ttcgacccca
    19381 acaattgctc caaagttttt ttgaaaaagc ttgagctggt tgtagatagc ctcattattg
    19441 gtagcagcta aaccagctac cacatcacta tgaccattga taaattttgt cacactttct
    19501 accacaatat ccacacccat agctaagggt tcttgataca aactggtcat aaaggtatta
    19561 tcagccacag ttagcacacc ataagctttg gcgagttgcg caagctctga aatatcacaa
    19621 attttcaaga gcggatttga cggtgtctct agataaatca tacgcgtatt aggcctgata
    19681 cttgctttta ctgttgctaa atcagccata tctacaaagc ttgtttcaat ctgataattg
    19741 ggtaaaatag ctgtcgcaaa ttgacaagtc cctccataga cctccaatgg gaaaatcaca
    19801 tgatctccag cttttaagag catcaaaaca gttgaaatag cagccatacc agatgcataa
    19861 gccaccgcat aagtcgcttt ttctaaacag gctaacccgt cttctaaagc ttcagtcgtt
    19921 gggtttgaaa agcgtgtgta caaatgtttt tgactaggac aatacagttc tgaattgtgg
    19981 aacgtcgaag tctgatagat gggaactgaa gctgctcctg taaactcgtc aataaccgta
    20041 tagccatgta ataaggtcgt attttctctc atttcgtgca cctccgcaat agcgttaact
    20101 ccaatgaact ctcttttaga caatggtccc accccaatgg ttgcacaagc caaaaagctc
    20161 ttcaaaaaag cgtgatctgc taacctataa gaaaagtagt ggacaccttt taaatggata
    20221 atgaattttt ccaacaatat catttttaga tcgattacgt ttattataca acctttagct
    20281 aagtttgcaa gcccttttat catctttgac caagctgcca acacctctcc agcaagttaa
    20341 agtatagcta cctttggccc catcgtctca aaagctcaaa ttatcacact gacagtccta
    20401 ccttgcatcg tcacctactc aatatggcaa tactgccatc aaaatttcat taaaagttca
    20461 taatttattt catttaatga aaaacagaac ttgttttcac catgcgtctt atagtacact
    20521 gctaaagtac tatttatttt taggagagaa atatagtgaa aaacaaatta tttttagttg
    20581 cccttgcgac cgtaactgtc ctagggccct ctttagcaac ccctcatcat cagaccgtgc
    20641 atgcggctga gcaggcaaaa gaaccccaaa ccagctcaat gacgtgtact aacgatggat
    20701 ctgtatgcgc tggctggcat agtttatcag aaggagacag tataacttca aattcatctt
    20761 caaacaccaa gacttccctg ttaaactggt tatggagctt aataacaaat ttgtggaatt
    20821 ctgcaaaaaa cttgttcatc taagatctct ataatcatat ccatggaatt taaataacca
    20881 tctcgtggcg gttttctaat cctaggtcgc cacacttctt attcagataa ataataataa
    20941 aaacattaaa ataacacttg atttattaac aaaaagttag tataatataa tcacaataca
    21001 aattaaagga gttataaatg aaaaagaaat tatctttatt tatgatcgca actgctgctg
    21061 ttgcaggcct tagccttgca acactaggaa gccaccaaac tgtttcagca aatgatgcta
    21121 gctatagtaa cggtttctac ggacacactg gtcctgacag ctggttactt tacacagtat
    21181 ggtaaaccta ataccgtaac atagaagcta tcttcttcgt aagactacgg ttatgaaaag
    21241 cctagagctg cgtaagcagt tctaggcttt ttgattggct aaaacgtttt tactaacgat
    21301 ccctcaagat taaaaagaaa aggcataccg cctataaaag gacaagccca aaacacaagt
    21361 cgtaacagtt gctagctaac caccaccaaa aaagctgtaa cacctcaaat ataattataa
    21421 ccagattaac attattaata attaatgtta tttatataag ataaagaaat gctttagtat
    21481 cggctttctt tgcctttttc ggtcaaacac ataacaaaaa gattaaaatg tgatattgaa
    21541 tcattaacat aatatgagta taatataggt atactataat ttaaggagtt acaaatgaaa
    21601 aagaaattat ctttatttat gatcgcaact gctgctgtcg taagccttag ccttgcaaca
    21661 ccagaaaacc aaactgtttc agcaaatgac acgcaagtta cttgtgtagg atgtccagat
    21721 tgtggtccaa aatggtcttg ttgtccattg acctgtatgc gcgatttgtt aagaggttgg
    21781 ggcaagtcag cagcatatta ttgcacttgg ggatattact actctgagta aaaattactt
    21841 gagagtctga gacagtagtc tcttgattat gctaaaaaaa caatggcttt gacgccattg
    21901 tttttttgtg tggccgagag gctttttcac aaactctttt tttattgttc attcccatat
    21961 cctaaaaaaa caacctttca cattagcagt taaaggctgt ttttaattca tttttattat
    22021 gacatgcttt gtactgttgt agttgcctgg ttattgggga agaattgctg tagccaatga
    22081 gagatcaaat cccatataac cttgaccgga gtcaagacgg gatgctgaga tttccagcct
    22141 tcattataac catctctgta cccatctgta tacccgtctt tataatgctg tttattagta
    22201 gtctctctga tatcactagg tggttctgga atggttggat tagttggggt caaatcagtc
    22261 caactagctt cttttccagc tgttttccct gcttcttctc cagcgctttt ccctttataa
    22321 tatgctgcat tagctgggtc attattctcc ccatctgcca taacaggtct tgctataagc
    22381 aacatagcag caaatagagc caccaagagg tagacgaagc gtagaggctg gtaagattgt
    22441 tgtttcatga taatagttat ctctcccttt tatgttatgc tttgcttgtt aaaaagcagc
    22501 ttgttagtat tatagattag aagcagctta gcttcaaggt cacgatgtga gaaaagaata
    22561 attttttgat tagtatatca aaaaagcttg tctatgaagg tatctcatag acaagctttc
    22621 ttctgtattt gtgagaggat taggcacaag gcctcaagag ataagcagca tcaaggtgct
    22681 tgacgaacct cttgaaccag tcctacttat aagtaatcga accatatggg ctcaaggttg
    22741 atcctgagat gttgacattg atttctttag acagtttcac atctctttcg tcgatcactt
    22801 ttcgccacca ttcccaagct aagccggtgc actctctagc catgatacgg atatttcgtg
    22861 aattagctcc tagtgggata actgtgctaa atggtgatgt cttactatac cagttgttgt
    22921 cccaacgtcg ttttgtaatc acttcttttc ctttgtcatc ataattgatt tcatcccaaa
    22981 ggatttcata ttgagcaaca taggcacctt gatgagacag gttaattttt ccactcgtgt
    23041 actcggtaga tgttgtttca acgtattcac ttctgttatt gacacccgca attttattat
    23101 ttttaaggaa aacactggtg tatgaaatag gataagctgg gttttttcta ctgaaggtag
    23161 cattatcttt gataacgttt ctaataacat caaagtcttt tgtgactacc ttattgtgct
    23221 ctgcagcatc tcctcctaaa acgacagctg taaatgaact attttctaaa atatcagagt
    23281 attttccatt agttttaaca tctgttcctt ttagagctgc actaaaggcc gcttcaacat
    23341 cattactttt agaacttgtt tctagtttga caaaaacagt tcgaccataa gctacgttac
    23401 tcacaaagag tggcggggct tcattgctga caccttttcg ttgcaactct ttaaaggtca
    23461 ctgatttatc aaacacatcc gcaggattat taggaaggtt tgctgatacg gtgtaaaaaa
    23521 tttgcttgta tgctgcaatc atcaccttct tttcaccttt tgaaatcgac ttgaaatcaa
    23581 tgcctaaagt accatctaag attttactat taacatttag agctgcttca atctgtgatt
    23641 tagaatatac cattgattca gtatattgtg ttctggcagg aagcgtatta ccaccagaat
    23701 aattatcatg ccattggtta acaagattat caatagctgt tgaaacattg gcataggtag
    23761 ggtcattgac ctcaaccgtt gctttgtctc ccatacctgg taaatcaata tggatttttt
    23821 gtgggtttcg cttggtgact accgcgtctg gtttgttttc ggtaaaacct ttattagcca
    23881 gctgaagggc tgctggatag gtcctatcag tgacagagtc aatgatggaa atatcgactg
    23941 gtgtagtgtt gatatttttt ttctttcttt caatgacaat aaatttatca gctttcttaa
    24001 cgccttcttt aggaacaaaa ttttcaatgg tttcaccatt tttagcaagt acttcaagct
    24061 cattataatt tagtgaataa atcttgtcat tgatttcttc agtgtgatct tcttcgctct
    24121 tttttttgtc ttctgacttt ttttcttctt tttctgcaga ttctagtggc atttctttgg
    24181 gagcaagctt aatcatatcg ttagagttaa gcatatcatc cattttctga cctgcttttt
    24241 cggtagttag ctcactactt tctggctttg gttgctcatt tgtcgttgtg gtttctgtac
    24301 tagcagtgtt ttgtttgttc gattcagcat tagcagtaac aagattacca atgataagag
    24361 ctgccgttag tagcccagcg acgcgactgt attttttaaa tgtttttttg ttagacatgt
    24421 ccttcatacc tttttatcat tctaaaatgt ttctattgtt ctttcgacca tatcaagcat
    24481 agtatcttga tagtgagcaa attctcctaa tttgtaatgt accatagcct ctctaatatg
    24541 cgcatctttt aacgctttgg ttatattagc tagtatgctt tgacggttta aataaaatgg
    24601 caagctttcg taataattta ataacatgtt ttcagcgttt agggtttgaa ccgcttggta
    24661 aatgtctaat gcttgaccgc tatttgacat ttctaataat gcttcttcga tgttagcttt
    24721 caattgggta tagcctttta tgaaagcagg gtaaacccca tactctaaaa taacttctgg
    24781 atcagaaata tctcccatat aggagtctct tctagtgata cgatactctt tgtcactacc
    24841 tatcaagtat ttttttaaat cattaacagt tacttctttt tgaaacattt tttgataatg
    24901 ttctaaactc tttggcacct tatacatatt gttacttcct atcttgcatt ctatcttgca
    24961 ttttcttaat tttgaagcga ccagttactt ttttatggtc accatttgga gcaatgatgg
    25021 taggatcaat aatttcaaaa tagcctttgg catcaatctc tgctaagaca gctttagatt
    25081 ttagatcttt aacactaatc gatgggaaaa ttaactcact ttctgaattt tcaatgtgtt
    25141 ttcctgacca cgtttggcaa ccaggaatgt tgagcgaatt tgctccaggg gtatctccgc
    25201 tagctgttcg gatatagcct agcttatcaa tgttttttgt tagttcagaa tcgtactcat
    25261 attgataaac tccatcgcta tattttccgc tatcgacacc ttttatattt tcaatttggt
    25321 cagctgattt taacttacct tgatcagtaa agagcaatgc catatcttca gcaaaacttg
    25381 tcacaaaaag accgttttct tgtcgaccaa tcgttgactt tcttttagca tcatcaaaag
    25441 ctaacatcaa atctttttta cttgtaacgt gagaagctcc gctttctttg tagacttgtt
    25501 ttagtttttc aacaaactct tttttgtagt ggtatttgcg gctaacgtac atccggtaat
    25561 actcagcacg tgacgcaaca gcagacatct gaacagcttt atcagcatac ttgtcggcag
    25621 tttcaataaa ctcatctgct tgtttatctg ttacccattt atctttaatt tctttaaact
    25681 ttttttcaaa aagatcgcgt tccatattgc caaaagtgac ctctgacaag gctaaaatat
    25741 ccttgttttg tccctgatgg acctctgtta cctcaatatc aatacgatca acatctaatt
    25801 ttttatcaat ctcaatgtga tgcggttttt tagtgtaggt gtcgcctaat tcaatttttt
    25861 gaaagaggac caatttattc ccgttatagt atttaacatc agcctttgca attctatttt
    25921 tcttgtagta attctccttg ctagatgtat ctccattcac aattaaaatt ctcccaatac
    25981 gcatcgggga aacaaacttg taggaaagta cctcaccaac gccaccacca ggtgaatttt
    26041 cctcccagac agtactcatt gagccgtcta atgtgtgcat gacatgattg tcttctttag
    26101 aggaagttgc gttagcttcc ataactttgg tcgtttcata tttgacatcg ctttttttat
    26161 tttctttgcc actaacagcg tacgttgtgc tattagctaa taaactgcta cccattgctc
    26221 cagccaaagc aatagcaaca cttgtctttg cgacaatatg agctaatgtt acttttttgt
    26281 ttctcatgta aaccacctta tattattttt aagtaaacta tacattaaca actagttttt
    26341 gtcaatgcga tttattaaca tcgttttagg cttttctatc aaaaaaatga acacaaaaaa
    26401 acaagtagcc aactgttaca agtttcaagg ggatatttcc atcttattat cacagaacct
    26461 tcacatctgt tctatttaaa ccagattaaa agtactagtt aaatatttga tagtagcacc
    26521 gttatcagta atagatctgt taattatttc tgattacaat gatgttaaca atagtcatcc
    26581 attggtttgt aaacacgctt cactgccaac taaaaaaggt ctgatggtaa tacttatacc
    26641 atcagacctt tgagagccta tagttctgca atttgataaa gttcaacttc tgctactgtt
    26701 tcagagccaa acatgttgag catcagcttc actttgttgt tttcgatttc taccacacga
    26761 ccttcttgtc ccataaaggc accgtctatg atctgaacca catcaccttc tttgatattg
    26821 gtatcaaaga catcaatggt ttgacccata gacaacaaga tagcacggat ttcttcttca
    26881 agaagtggtg ttggttttga tcggttcccg tgtgatccca caaaacctgt aacgtttggt
    26941 gtgttacgca caacaaacca agcttcatct gtcatgacca tttcaaccaa gacataccct
    27001 ggaaagcgat tttcttcgat ttctttggtt tgcccatttt tttcaacgtt aaccgtttgt
    27061 gtcggaattt cgacgcgcaa aatattgtcc aacatgttat aggtttgggc acgttgcaag
    27121 aggttttctt ttactttgtt ttcgtagcct gagtaagttt ggaggacgaa ccagccctta
    27181 tcaaatgaat ctagcatgat gtttcctttc attataaaaa agcccttttt ttggcttttg
    27241 cgctgttttc tatcaccttc attataataa aagacagggc tggtgtcaac gtatctgtgg
    27301 agctacttga cacctttatt aaaaagtcca aagtcggttc gtgacgactc tggacggtat
    27361 tgtacagttt tagttttcag gaactgtgat ggttccttcg ataatcgctt ttttagcagt
    27421 ctcaatagct tttttagtgt cttgtgtcag agcatccgtt gtaaggctaa caccaccttc
    27481 ttttaaacca aaagtggtta tttgaccacc tgggaattgg ccttctgagg tttttacggc
    27541 tacttttact aaagcttttc cgacttcctt gatacttgag gtcaaaacaa aattagctga
    27601 cttgccatct tttgtggtgt attttccatc ttcactttgg tcacggtcaa caccaataac
    27661 ccaaacctta tcttcttctt tacgtttttc gttgatagac ttagcttcgc taaagacacc
    27721 cgcccctgtg cctcctgctg catgataaat aacatctgcg ccttcagcat attgagcagc
    27781 tgcaatcgtc ttgcctttgg cagcatctgc aaaagagcct gcataagcaa ctcttacttt
    27841 gatggtatca tctactgatt tcacaccagc ttcaaaacct ttttcaaagc gcttgacaac
    27901 atctccttcc ataccaccta caaaaccaac ttgcttggtt tttgtcgttt tagctgctgc
    27961 aacaccggct aggtatgccg cttcatggtc tgaaaaggtg atacttgcaa catttttttg
    28021 acctttaatc acatcatcca caattgcaaa atggttgtca ggattgtttg cggctacttt
    28081 ttctacagcg tcatgtaatg gaaatccaat cccaaaaaca agattataac cattggttac
    28141 tgctgactca aggtttgtgg taaactctga ttcattggct gattggaaat aatcatagcc
    28201 ttttcctttt tcaagtttat tttcttttcc ccaagcttgt aagccttccc aggctgactg
    28261 gttaaatgac ttgtcatcaa tacccgtctg attagtgatc atagcgattt ttaatgaatt
    28321 gtcagttgtt tgtttagagg agttattggt gcagcctcct aatgtgaata gggcagtcga
    28381 aacaagacca agtgacatta cttttttgtt catgtgaaaa tgaacctcct aaatgtttaa
    28441 tcctaaagtg atgtgtcatt gttaacgttc tacttatgct ctagaacgaa aagtttagta
    28501 tatcaaaaaa atcagtttta tcctactatt tttgctgaaa aatctctttt tcaccaaaat
    28561 gaccgaacga gaaagtgaga tcaactggta aaacccgtta tctcctcaaa aatcacctct
    28621 taaggagata agacaacgac aaaactcaag ctactagtaa tagcatgact aactacacaa
    28681 tccaacaaac ggtaaaaaga gctggaaacg ctatccaact ctttgtcata ctagacacct
    28741 tagatagatg cccagaccga ctcaagaata ttggtctgtt cacgtcctgg accaactgag
    28801 aaggttgaaa tgcgaacgcc aaccaattct ccaacacgac ggacatagtt gcgggcattt
    28861 tcaggcagct catcaagact acgaacaccc gtgatgtcct cttgccagcc tggtaattct
    28921 tcatagattg gtttgcaacg tttgagttgt tcaagacttg ctgggtagtg atcaatacgt
    28981 tcaccatcta aatcgtaagc cacacaaatc ttaaccgtat caagtcctga aagaacatca
    29041 atggaattga gggaaaggtt agtaatccct gacacacgac gactatggcg cataacaact
    29101 gagtcaaacc aaccgacacg acgtggacgg ccggtcgttg tcccgtactc atgacccact
    29161 tcacgaatgc gctcacccac ttcatcaaag agttctgtag ggaatggccc atcaccgaca
    29221 cggcttgtgt aggctttaca gacaccaact actttgttga ttttacttgg gccaacccca
    29281 gaaccaatag tgacaccacc agcaactggg ttagatgagg ttacaaatgg gtaagttcct
    29341 tggtcaatat caagcataac cccttgtgca ccctcaaaaa gaacacgttt cccagcatca
    29401 agtgcatcat ttaaaattac agaagtatct gtcacatatt gtttaatttc ttgaccataa
    29461 gcatagtatt cttcaaaaat agcatcaaaa tcaagaggtg tactgtcata cattttttca
    29521 aataaacggt ttttctcagc taaattaatg cgtaggcgct ctgcaaagat atccttatcc
    29581 agtaaatctg cgatacggat accaactcga gcagctttat ccatataagc tggaccaatc
    29641 cctttaatag ttgttccgat tttattgtca ccttttgcat cttcttgcag ttgatccagc
    29701 tggatatgat aaggtaaaat gacatgagct cggtctgaaa tgcgaaggtt atctgtcgtc
    29761 actccttcat catgcaggta cgccaactct ttgaccaaag atttggggtt gaccacaaca
    29821 ccattaccaa taacggaaat tttttgcggg aagaaaattc ctgatgggat caagtgaagt
    29881 ttaaacttct taccatcaat cacgatcgta tgaccagcat tatcaccacc ttgatagcga
    29941 gcaattactt ctgcatccgc tgacaagaaa tcagtgattt tacctttacc ttcgtcaccc
    30001 cactgggtac caacaacaac tacagatgtc atatcattgt cctaactttc tgaaacactt
    30061 attctcaata aaaatcatag gcaagctaac cactgcttaa aaaccaaaaa gaaggtcaga
    30121 tctgtaagat cagcaatgta gctcttgata tttaaagaaa acaggcagga atctcacctg
    30181 ctagcatata tcttacaact tattatagca aaaaatgatt ttttctgcta gatttcttag
    30241 cattattaag gaaaaagaga aactcttttt ttaaccatga taaacaatcc taaaaaaacg
    30301 aacattataa gagaaaagct tttaacctcg tttctcctaa actttagcag ggttaaacga
    30361 ttctcttaaa acattagctc ttttctttca gaaatagcgg ttttaaggta aaataatagt
    30421 aagttacctc tatgagatag ccagtaaaaa catatcgagg aaatatcaca tgattaaaaa
    30481 gtatgaccgc acgagcacaa aaaagaaatc actaaactgg atttggctaa tcatcgcttt
    30541 ttttatgatt tcaagcttta taggtggctc ttcttttact gagtcacttc tagacatttt
    30601 gcctgccata gccattggtg gtacaggtta tgctattttt cgtgttagat ctcatcaaaa
    30661 gcggctagta aaggccaaga ttgccaaaca attagaagat ctaaaagcaa aaattcagct
    30721 agctgatcgt aaagttcggc tgttagacac ttatctggca gaccatgatg acttccaata
    30781 taacgtacta gctcagcaat tattaccaca acttagtgac atcaaagcaa aagctattac
    30841 gttaaaagac cagttagatc cccaaattta tcgtcgtatc accaaaaaag ccaatgacgt
    30901 tgaaagtgat attacccttc aactcgaaac actgcagatt gccacaactc ttaaccctca
    30961 gcctttgaaa accccatctc ctaatcttat taacaaagca cctgagctca aaccgtatta
    31021 tgataatatt cagacagatc atcaagctat ccttgctaaa atccaaggag ccgacaatca
    31081 agaagaattg cttgcgctgc atgatgctaa catgaggagg tttgaggata tcttaacagg
    31141 ctacttaaaa atcaaagaag agcctaaaaa ttactataac gctgctgctc gtttagaaca
    31201 agccaaacaa gctatccaac aatttgatga agatcttgac gaaacccttc gtcgactcaa
    31261 tgaaagtgat cttaaagatt ttgatattag ccttcgaatc atgcaaggtg ctactcaaag
    31321 acgaacaacg catcaccaaa aagactaaag gagtcccttt atggcagatt ttaattttga
    31381 tattgaccaa attgctgaca atgctgttgt caaaaccgat aaaaccaccg atattatctc
    31441 agacttacca actgacacaa atggccaaat ctcttttttt gagaaactaa gtgctgacca
    31501 gcagacagct attactgcca aagccccggc attggtagat acctttttgg cagaccaaaa
    31561 tgcgctactt gattttggtc agtctgctgt tgaaggggtc aatgcaacgg ttaatcatat
    31621 cttggcagag caaaaaaaat tacagattcc tcaggttgat gatcttttaa aatcaaccaa
    31681 tcgtgaactc aatggtttta tcgctaaata caaggatgcc acacctgtgg accttgataa
    31741 aaagccaaac tttcttcaaa aactctttaa acaaagccgt gataccctgc aagagtttta
    31801 ttttgattct caaaacattg agcaaaaaat ggatagtatg gctgcagcgg ttgtcaagca
    31861 agaagatacc ttagctcgca atattgtctc agcagaattg ttaattgaag ataatactaa
    31921 gtccattgaa catttggtag gcgtcattgc ttttatcgaa gctagtcaaa aagaagctag
    31981 tcagcgcgct gcggctttac aaaaagacct aaaaaccaaa gacagtgcta caccagacta
    32041 tcaaataaaa gctgatcttt tggcaagaac taccgaagtc atcaacaccc tcgaacaaca
    32101 acacaccgaa tacttgagtc gcctctatgt tgcttgggcg acaactccgc aaatgcgtaa
    32161 tctagtcaag gtctcttctg acatgcgtca aaaactaggc atgttacgcc gcaataccat
    32221 tccaacgatg aaactatcta ttgctcagtt aggcatgatg caacaatcgg ttaaatctgg
    32281 aatgactgct gatgccatta ttaatgctaa taatgctgcc cttcagatgc tagcagaaac
    32341 aagtaaagaa gccatcccag cattggaaca atcagcacaa agccctactt tgtctatgaa
    32401 atctgtgaca agtctggcag aaagtttagt agcacaaaat aatggcatca ttgcggcgat
    32461 tgatcatgga cgcaaagaac gagcacaact agaaagtgct attatcaggt ctgccgaaac
    32521 cattaacgat tctgtcaaac tacgtgacca aaatattgtg caagcacttc ttagtgaagg
    32581 caaggaaact caaaaaacaa ttgataaaac aactcacgca taagctaaac atcacctgtc
    32641 acgctactta aaaagcaagc catcagttag gttaatccct ttgatggctt gcttttttac
    32701 tacttaacga ataatcgcct tgtttgatat aacacttatc ttttatgatg ttattttcat
    32761 aatacgaaca aggctagcac gctcggcctc ttctaatttc aactcaatgt agtgaatcgt
    32821 ctcttccagt tgcgggataa tagcatactc taagccattg acgcgacgtc tagtcttttc
    32881 aatgtcatca gccattagtt gacaagtttt ttcaacttca gctaaccgta agagcttttc
    32941 taatagttct tttgtctttt ggatagtatt gtccatctca ctatttgagc tgaggtaact
    33001 ataagcaaac tcaccttgtt cttgctctct tgccgtatta gactggacat ggaatttagg
    33061 cacattgaca ctcatgatat tttcaatatc aatccataag gtcacttcat ggactggaac
    33121 agcaaagagt tcttctacca ttagactatt ttcagatgct tttgccaaga caaactcctt
    33181 catattggca gcaagttctt tttcgatagt ttgacgaagc tcattgtttt cacgaattaa
    33241 atcaacgaag cggcgcatca attcatctcg cttgtccttt aataatttat gacctcttgt
    33301 agctgtcttc aatcgatttt tcaagttact aagttccatg cgtgttggtt taacatttaa
    33361 tcgtgccatt gatataactc ctttcgtcaa gggcttgaaa ttgcatggtc aactcaatca
    33421 tttgcaacaa aaacctttgt catcgtggta tctgctttag gcaaataacg atctaacata
    33481 tcatctttaa tgcgttttaa ttcggtacgt ggcaaaatcg acaacaattc ccaacccaag
    33541 tcaagacttt cttcaatgct acggttggtg taaaatcctt ggttaatgta ttcttcttca
    33601 aaccggttgg taaaggccac atatagttta tccgtttcag aaagggctga ttcaccgagc
    33661 acaacagcca attccttagc ttgttttcct tgggcatagg ctgcaaagag ctgattcatt
    33721 gtggcagcat ggtcttgtcg agtcttacct tcacctgagc ctttatcttt taggcgagat
    33781 agcgatggca agacattaat aggcggtcta aaaccatttt tatatagttc ttgcgacaaa
    33841 ataatctgac cttcggtaat ataacctgtc aaatcgggga tagggtgggt aatgtcatct
    33901 tctggcatgg ttaaaatagg aatttgagtc actgaccctt ttttcccgat caagcgacca
    33961 gcccgttcat ataaggtgga gagattagta tagagatagc caggataacc tcgtctgcct
    34021 ggaacttctc tgcgagcggc tgatacttct cgtaaggctt cacaataatt tgtcatatct
    34081 gtcatgatga ctaaaacatg catacctttt tcataagcca agtattcggc tgttgtcaga
    34141 gctatacgtg gtgttgcaat gcgttcaata gcagggtcat tagctaaatt gataaataaa
    34201 acagatcgat caatggcacc cgtttcacgc aagtcattca taaagaattc tgcctcttca
    34261 aaagtaatac ccatagctgc aaacacaacc gcaaagttat catcagagtt tagaacagtt
    34321 gcctgacgag caatctgtgc ggccaattca ttatgaggta accctgagcc cgaaaaaact
    34381 ggtaactttt gaccacgaac gagggtattg aggtgatcaa tagctgagat acctgtctgg
    34441 ataaattcgt ctgggtaatc acgagcgaca ggattgatcg cttgaccatc aatatcaagg
    34501 tatttttcgg gaataagttc tggaccgccg tctatgggct gccccattcc atcaaagatg
    34561 cgccctacca tatcttctga tacggctaat tccaaaggat gccctgtaaa tcgaaccttg
    34621 gttttggcta ggttgatgcc agttgatcct tcaaatagct gtaccatagc cttgtcacct
    34681 tggacttcta agacttgacc tttacgcctt tctccattat gcaaagtaat atctaccaat
    34741 tcgttgtagt gcactcctgc aacttgatca acaatcatca aagggccaac cacttcgcta
    34801 accgtccgat attctttgag aacgctcatc taaattcctc cctttgctaa aacgtggtga
    34861 atctctttgg taaccttctg agtaagccct ttaatctgct ctaagttttc ttctgggata
    34921 aatttgctgc gtgcgatgcg atcgcgtacc tgagcagtgc cttccataat ctcattaaaa
    34981 taagctccca aagaaagggc tttgctggct tcctcattaa aggtcaaaat attggttagc
    35041 atggcctctt gtttcggaaa ggaagtaaag gtatccaccg aatcaaaggc attttgctgg
    35101 agataatcct cccgaatttg ccgggcaaca gccatggtca aacgatcttg ttcagacagt
    35161 gaatcaagcc ccaccaagcg tacaatttct tctagactag cttcacgctg caaaatagcc
    35221 atggcacgag ttaccttgtt ggaccaatta gattcctgtt tacggtcaat atagcttcct
    35281 acatcatctt gatacaatga ataagacgtc agccagttaa tcgctgggaa gtgacgccgt
    35341 tgcgcaagag gcgcgtcgag cccccagaaa actttgacaa tccgaagggt gttttgagtg
    35401 acaggctctg aaatatcccc tccaggagga gaaaccgcgc cgatggctgt aatggttccc
    35461 tcacgttctt gacttcccaa ggtccgaaca cgaccagccc gttcataata ttcggcaata
    35521 cgactcccta agtaagccgg gtagccttca tcaccaggca tttcttgtag gcgtcctgac
    35581 atctcgcgca gagcttctgc ccaacgtgat gtcgagtctg ccatgatagc cacagaatag
    35641 cccatatcac ggaaatattc ggcaatggta atgcctgtgt aaatcgacgc ttcacgcgct
    35701 gctactggca tattagaggt gtttgcaatt aacaccgtgc gctccataat ggactggcct
    35761 gtatttgggt caattaactc tggaaactca ttcaaaacgt cggtcatctc gttgccgcgt
    35821 tccccacaac cgacataaat aacaatatca acattggcaa atttagctat ttgatgctgc
    35881 acaactgttt ttcctgcccc aaatggtcca ggaacagcag cggcaccacc ttttgtaaca
    35941 gggaaaaagg tgtcaataac ccgttgacct gtaaccaaag gttcgactgg gataagcttt
    36001 tgagcaacag ggcgactttg acgaactggc caagtctgca tgaggctacc ttggtaaatg
    36061 gaaccgtctt cctgcttaat ttcataaact gtatctgtta ctgtgaagtc acctgcatga
    36121 atagccacca aggtccctga aactttataa ggaaccatga tacgatgctc aatgacagct
    36181 gtttcttgca cagttcctaa aatatcacct gcaactactt cttgaccaac acttagcttg
    36241 ggaataaatg cccacttagc ctttcgatca agacttggga tagccacacc acggattaaa
    36301 aagtcgctgt ccgttgcttt ttgaaaacga tcaagcggtc gctgaatacc gtcaaacatt
    36361 tctgaaatca ggcctgggcc taactcgacc gacaaaggac aaccagtagt cactactggt
    36421 tctcctggac cgatccctga tgtttcctca taaacctgaa tagacgcttg atcgcggcgc
    36481 atttcaataa tttctccgac taagccaaga tgtcccacac gacaaatatc ttgaatatta
    36541 gcttcttgca tcccagaagc cacaacaaga ggtcccgaaa cggttattat ttttccttgg
    36601 ttcacctttt tcctccttta ttttttccaa atcatactaa ctaatctata ctgtcaaggt
    36661 ttaatgtgac tattttgaat agtcagcaaa ctatcctatg cgttcagctg ctaacttcac
    36721 caattttcct gtccttgcat ttcatcagtt agctactcat aagcctcaaa cagagtataa
    36781 gtcctctttg cctaacgtct tagtgttata aaatattttg accgacagcc ttttcaacca
    36841 tggcttgtat gcgtgacagg cctatccctt gcgctccttg atgagttggt aatggtatga
    36901 ctgctggtag aacttgattg tcatagtgag tgagggcttc tggaatagct gcagcaatat
    36961 cttctgtgat ataaataata ccaaagtctt ccattgctag ttgacgcaac tggttaatag
    37021 cgtcttgtgg ttttattact ggaaaagttt gaaacccaat catctgaaaa ggtaaaataa
    37081 catctctgtt accaattacc ccgactttat acaatgtttt ggtcatgcta ttggtctcat
    37141 cctttctatg acacgttctt tgggtaaatc attagccaaa gcagccgcta aaagccgtag
    37201 attttttact tcaaacgatt tagcgagcaa aaagcgagca agcacataag gtccatcgac
    37261 ttgatattta gccttcgcaa ataaatggta aaggcattca tcaacaaggt attctaactc
    37321 agtcgtttga agagtgcctt ggcgaagttt ctctgaataa ggttttaggg ctgtcatata
    37381 gctatcaggt gttacatttt caaaccacgt catcagatcc ttatcatctt caagtgtgat
    37441 aaatgtttta cttggtctac ttgcttcatc agataataac tgcatcatga aacttattgg
    37501 tttattttgc gatttggccc gtctgacggt aatcagatta tacaaatcaa tcacaataag
    37561 aacagcttgt tggaaaacag ggtcctctaa tctttgtgct atttgtttca acgccttaaa
    37621 ataagctaag tcagtaccaa tttctaagac gcgtatgtct tgataatctt gatattcggc
    37681 ccaaatggac tggatttctg ttaccacctc ttttggaaac tcatcagaag tcatcgtcct
    37741 aatcaaatgt tccaaagcca ccaaaggctt gtcaccaatt ggtagtaata agtgactcag
    37801 atctgcttga ctagctttag cttttaacaa cactttgaca ttatgatagg tgtaacgcaa
    37861 ggtaaataat tgtactatgt caggttgagg ggtttctgca aaagcccagc gataggtctt
    37921 ggttagctca gccattaaga tagattctag ccgatccaaa tcgttgagat cgtcaacagt
    37981 taggtgatag actgactggt gcaacagtcg agctaaggtt gttgtattgg gagcctgcaa
    38041 tagcttatca aattgctctt tagtcaataa ctctttttct ttgacactga tagtagtgtt
    38101 taattctaga aatgttggca tcgctagtca aacctcctat atctctgaaa aaagcatatc
    38161 aatgatgcgg gagctttctt ctttaagaac tgattctaaa aggtaacggt ataaataatt
    38221 gtcatcgact tggtctaaac tgactaaaaa tcctgcttcc tgaggaatta gctcttgatt
    38281 atagcttaat tgaggaaacg ctaatctcag ctcagcacaa tcataagaac tgaacttttg
    38341 ccctgttttt tcgccaaaag taacttgcaa tggctgttct ggatatttag acaagacttg
    38401 cttcaaaaaa gccaattctt cttccttaga aaagttcgtc atcttctcca aagaagcttc
    38461 aaatagctcc tttatactgt cttgttttaa tgctagtaga gcttgtcttt cttggttttt
    38521 gagttgctgc tgcgcaacct gaaattgctg ttcaagagct tttaactctt tttgacgctt
    38581 tgcttttaaa tcgtgtagtc cttgctgttg acgctcttta aagtcagtat caagactatc
    38641 tgtcgctatc tttaggcact gttgaccttc ttgatgtgct ttttcaagaa cattttgtct
    38701 taactgtgtt atatcattca cctaatttcc cccttttgat agtgttagtg gtgccaccgt
    38761 tttttaaaat ctagataagg accgttacct tatccaattt tatttaagag aataaaggag
    38821 acgacaaagg caagaatagc ataggtttca accatagctg ctaaaatgac acccttcata
    38881 aaatctttag ggcgcttagc caagatttgc ataccagcta cagaaacatt gccttggtgc
    38941 ttggccgaaa aataaccaac aatagcaatt ggaagagata ccaaaaagta agctaatcct
    39001 tggttaactg aaagttctgg tgtcagtttc atccaaatta aaataccaat agcaaagccg
    39061 tagattcctt gactaccggg taacagttgc aagataagcg cagacgtaaa tttttcgggt
    39121 tcttctttta agagcgcagc tgccgcttga ccacctttac cgacaccata agctgagccc
    39181 atgccactaa gagctacggc taaaacaatt cctaaggctg cgaagaaaac gccaccatgt
    39241 gctgtaaaat aatgtgctaa atgttccatg ttaattgtcc tccaaatctg tttcttcatt
    39301 gacattgaca taattatcag ctaactttaa aggattgaaa gcttttcctc caccttcata
    39361 aaattttcca aaaaactcta cgaaaataag acgagcacca tgcacatatc ctgaaagcat
    39421 tgataagaaa atattgatcg catgcaaaag aataaaaata aagataccta ctgtaaagcg
    39481 cgtgactggt ggaaaaatcc caacaatcat gttaaaagcc gctccaatac tagctccaga
    39541 aagccctaat gccattagac gagtaaagct aaccaaatca cttaagtaag agctaatgcc
    39601 atataaatta tataatccac tacctagacc taaaaggctt ttgcttttta gtaaagaaac
    39661 aaccaaaatc ccaaaggcat tgcccaatgc aagccactta ccaataaccg ataagtaagc
    39721 aaggccagat acattcttac caagaataaa taacagcagg cctaataaaa taaaagtcca
    39781 agctagactt gacgtatagg ccgtagcata tgctttcatc cttacttgtt gccatgctcc
    39841 taacaaaagc ccaaatataa gtgtaacaaa cccaaatagc aaagaaacaa ccaaaatagt
    39901 aatcacatcc gttttagttg ataataatgc cacaggtaag tcaaaaccaa agaaagaacc
    39961 gtaaataagc ccccatatgg ctactgaaat agctaaaata ttaaagaagg tcactaaccg
    40021 tttactggtt ttttggagat taaaaaagac caatgcagct agtgtcacag catatagcaa
    40081 caaaccatag cccaaatcag ctaccatcat gccaaaaaaa gtaaggtata aaggagctaa
    40141 aaaaggtgtc ggatcttttt cttgatattt tggcaaagcg tacatttctg taaccaattc
    40201 aaacggtgca atgtagcggt gattacgtaa cttaatggga acatcttccc aatcgtctgg
    40261 cgtgacatcg taactatcca gataaaacat atcacctagt gtcttagtca ttagcccttt
    40321 taattggtta accgaatcag cttctatcca cccttctaag gctattagat gtcttgtacc
    40381 gagtaattgc tttttagttt gctgccgttg atactgacta aggacatagt ctatttcaac
    40441 ctgtaactgt gccagtatct tttgagactg cctaagctct tttagtagac tatctttttc
    40501 agctagccaa ttcgttagta actctttgtt tatacgaagt tgttctgatg gcagtagctg
    40561 ttctttatat aataagggtt taaactgata tttctgtaaa tgaatcgtat cttgtgcttg
    40621 ccaaaataag acaaggccgt actcaaattc tgtatggtat acttcctcaa taaaaagacc
    40681 tttgtgctgc tttagctgat gataaaaatg atcatttgct gtactaggaa tcgttccaac
    40741 tcttgccgac aaaaaatgaa attgcgctaa gtcctgaggc aaaacagtca aggaacgcca
    40801 cttttctaac tcctgacagt attctgttag ctggtctatt ttgttttgca gctgctctag
    40861 tcgttctatc ttttgtgaca tcattttcaa agccgtttga gctgcttgtt gtcgatggcg
    40921 ttcttcgaac tgtttaaacg aaagagatgg cgttttttgt ctcaaggctg ttaactttcc
    40981 tattggtggt aaaaacgctg ataagctagc aaggccgttt tctaactctt gttggctttg
    41041 taaaaggtat tgtaaagctt catcatctag tagaggtttt tgatgcgtca gatcgtattg
    41101 gacgatttgt ggcaacttga cgttgccttt gttaaaagca tcttgccaat gttttagttg
    41161 cttcatgtca cgaacttcaa ctaattgaga ttgttgaagt gtttttaaaa caagatcaag
    41221 ataatctttt tcaaaaacca ttgccagctt tttcatttga ctaatggcca tatgtctcca
    41281 ccaccttatc cactaactcc ctaacaacgg ttagcttatg ctcttctaaa atggcatgaa
    41341 gggctagctg ttgcgtcgct aacctctttt ttgaagcttt ttgttcttga cgattagctt
    41401 caaaaaacac ctcacgttta gcttctaata gtagctgagt ttcttgttcg tactcaagga
    41461 acagctgctt taactcttct tcttttttgt cagctagagc ttttttctta gcatcgtaac
    41521 tgtcataaat tgttctagcc tcttgttcga tactattcat tagtgacaat agggtattca
    41581 tcacatatca actccttatc tttatctatt gctaattctt tgactcacta ggtacttaaa
    41641 aacaaagcta caagaaaggc tacttttggg ggttaatcaa ccagcgacta taagctagag
    41701 ctaaatctct aacctatcta ctaacagctt ttttgagata ggataattgc ttgattaagc
    41761 gaacagtatc ttcaagttaa gcgttttcaa ttctatccat aaaaacaatg gcttagttca
    41821 gaaaaaggtt agctaaaaac ctgtcaaagc agtaagatga cacagaactc taaagctttg
    41881 tattgctgtc tttcgtgtat tatcttatac ttctttaggc atttagtaac cttgatctag
    41941 ttgacggtaa ggaaccttta ccagccaata gccaagccaa tgtgataaca gtataactgc
    42001 tatcgtgtta aaagtcctgt caaaaagatg tttattagtt tctcgcttgt aattcttgtt
    42061 ttgttactaa acggtaattt ttaaagacag aacctccaat ataacccaca attaagctga
    42121 taataataca tccaagggct gttatcaaca ctttagcgac aggattatag gcaaacatca
    42181 ccgcaaaacc ggcaatcgga gtagctgtcc ctggcgtatc attaaccaat ttcattaggg
    42241 cgatcaaaac accacatgca gcaccaccaa cgaaattagt cacataaatc ggaatgggat
    42301 tggctgacgt cacatctgcc tgagtcaacg gttcaatagc aaaagcaata ttgtcttttc
    42361 tttcacccaa ttttaatcgg taaaaaagaa caccattcat aaaagaagag ccaaagacag
    42421 ataaggcacc tatagccatc ggaatacctg tcagaccaag catggctgtc aaagccattg
    42481 atgacagcgg agctgttgct acaacaacaa tcgtaccgcc tagtatcatt cccatgataa
    42541 tagggttgct attagcactt gaggtcagga tgtctccaat agtcaataaa gtactattga
    42601 tcactggcgt aattaactgc gccaagaaac gagttaatgg tgcaccgaca ataataatcg
    42661 agattaaatc aagcccacca ggaatatttt tttccatcca cttgattaaa aaagatacca
    42721 catacccagc cacaaaacct gggataatac ctgttcctga aactgacaaa ccaatcaaaa
    42781 cggcataacc tggagacaca ccaattgcaa tggcaaccaa gatagcagca gctacccctg
    42841 ataatgagcc atttgctccg cctacttcac ttagaaactg aaagcctaaa accttaccaa
    42901 aaaaagaatc ttgaaaggct tcaactaaaa aagaggcaca agctgccgaa gccaaagctc
    42961 ccattgcctt ggcaccatga ggagccttat agttaaacaa gctaaaaata gctaaaacaa
    43021 gaatcaaaag acttgttcca ataataatat ccataatcta ttccaccaat ctatctcatt
    43081 acataagaca ctataactgc ttcactctgg caacagcttc gacttcaatc aaagcttgca
    43141 aaggaagcgc cgacacttga accgctgtcc gaacagggta aggctcttca aacaaatcag
    43201 tcatgacatg atttaatatt tccacattgg tcacatctgt taaatacaca tttagcttat
    43261 agatatgatt caagtccaac ttttgctctg ccaaaataga ttgtaaatta acaaaaacct
    43321 ggcgacactg tgcctcaaaa ccatctgaca attgacctgt cactggatta aggggtaatt
    43381 ggcctgcggt gtatagaaaa tgtccttcaa tcgtataagt tgaataaggg cccattggtt
    43441 ctgggtagct cttcatatac gaacctcctc attgataata tagttaaatt ttacaggaaa
    43501 ccgcattcac agtcaataat aaattctctt tttgataaaa aattattatt aaggtgattg
    43561 gcaattgttt tttagtctaa aatgtattac tatatttgta aataagaaga tagatatagg
    43621 aagtttgaca tggataaaga aacgctaaac tactggaaaa cggttatcac atttcttcac
    43681 gatgtcttag gtgataacta tgaaattatt ttacatgtga ttgataaaaa tgatatctat
    43741 attggagaac tcgttaatag tcatatcagt ggacggtcta aacagtctcc tctcaccacc
    43801 tttgctcttg atttgattac caataaagtc tataaggaaa aagattttgt gaccaattac
    43861 aaagcaatcg ttagtcctca gcacaaagaa gttcggggat ctactttttt tattaaagat
    43921 aaaaagggta atctcgaggg aatgctatgt attaacttag atatttcagc ctatcaagga
    43981 gtagctaagg acctcttaaa acttgtcaat ctcaatttag aacatttcat cccaactgct
    44041 aaagagccta aaacagtcac accccagcca gaagaagctg ttgaaatttt aacctctaac
    44101 atccaagata ttatcggtca aattattgat ccctcgttac tgagacacaa tgtacatctt
    44161 agccaagatg tcaaaataga cattgttgcc aagctttatg aaaaaggagt ctttcaacta
    44221 aaaggagccg tctcaaaagt cgctgatatt ctatgtattt ccgaaccaag tgtttaccgc
    44281 taccttaaaa aaattgaagc cgatcaataa ccaaatcacc ataaaacgcc agttaaaagt
    44341 atttcagata agcatcacaa tctctgatat accgatgact gacgtttttt ttactcaata
    44401 tgcctttatg gcaagaaaag gctactggtc attaaccttc agctatgctt agaggtacac
    44461 ctgtcgcagc ttgaacctca tcaaaagtaa acccttcact gatttcaatt acttggatac
    44521 catctggcgt tactttaaac acacccattt ctgtgataat aaggtcaacc acaccttttg
    44581 ctgttaaagg taaggtacat tcttttagga gcttatgctt tcctttattg gtatgctcca
    44641 tagctacgat gactttttta gcaccaacta atagatccat tgctcctccc ataccaggaa
    44701 ccattttgcc tggaatcagg taattggcaa tatctccatt ttctgcaatt tctaaagcac
    44761 ccaagacggt caaatcaaca tgaccaccac gtataatgcc aaaagaatct gcactattaa
    44821 agaaagcggc tcctggataa accgtcacag gctgtcctcc tgcattaacg atagtcgaat
    44881 tcggatcatc tgacttagga cctagtccaa taaacccatt ttcagattga aaatacactg
    44941 tcatttcttc aggcacaaaa tttgctactt tggttggtag accaatacct aaatttacca
    45001 aagtaccatc ttctaattct ttggctacac gctttgcaat acgattttga atctcttctt
    45061 tacttaatgc aaccgccata actatactcc ttctttaaca atgtaatcta caaaaatgtg
    45121 tggtgtatga acgcattctg gttgtatggt gccgactggt actatttctc gcgcctgaac
    45181 aattgttgtt tttgcacaag ctgccatcaa ttgattaaaa ttattttcag aaccactata
    45241 ctgtaaattt cccatttcat cagctttatt agcaaatatc aaggcgacat ctgcctcaaa
    45301 tgggtattct aagagatagg tttttccctt gatggttttt gtttccttgc cttctgccac
    45361 taaggttcct acccctgttg gcgtgtaaaa gccaccaatg ccataagctc cgatgcgaat
    45421 tttttcggcg aaggttcctt gaggaataag ctcaatcgtc gtttcaccgg cagtcatttg
    45481 gcgcccagct tctttattta atccaatatg tgttgcataa atggttttga attgatgatt
    45541 agccaccatt tttccaacac cgcgatcaac aaaaccagca tcattacaga tcaaggttaa
    45601 gtcttttgtg cctttgtcaa ccagtgcatc aattaacgct tctggtgttc cattagccat
    45661 aaaaccaccg accataatcg tgtcaccatc tttaatatga ctgatagctt ctgctattgc
    45721 tattctttta ttctctttca tacggttacc ttctatcttg ttacgatgat actttgacct
    45781 tggccaccac caatacaaag cgttgctaag ccatgtctca catcacgctt agccatttct
    45841 gctaataagg tgactaaaat acgagcacct gatgctccaa tcgggtgacc caaagcaata
    45901 gctcctccat tgacattaac tttttcattg tcaaaaccaa ggtctcgtga aaccgctaaa
    45961 gcctgagcag caaaagcttc gtttgcttca atcaaatcaa tgtcatcaat tgtcagctga
    46021 gcctttgcaa gagccttttt cgtagcaggt attggtccgc agcccataat acttgggtct
    46081 acacctgcac ttgcataact agtgatttta gctaaaatag ggagccctaa ttcttcagct
    46141 ttttctttac tcatcaataa aattgccgct gctccatcat tgattcctga agcattacca
    46201 gcagttactg tcccctcatc tttgataaaa gcagggcgta actttgctaa cttatccact
    46261 gtcgttccaa atttagggta ttcatcttga tcaatgagca aaggttcacc tttacgctga
    46321 ggaatagtga caggtgcaat ctcttctttg aaacgacctt tttctatggc aagttgggct
    46381 ttacgttggc tatcagctgc aaaggcatct tgttcttctc tggtcagtcc atactgttgc
    46441 acaatatttt cggctgttat acccatgtga taacctgcaa aagcatcact caacccatct
    46501 ttaagcatgg tatctactac tttactatct cccatacgag atccccaacg ttggccttgt
    46561 aaaacgtaag gtgcttgact catgttttcg gtaccaccga ctaaaacaat atcagcgttg
    46621 cccgtttgaa tcttttgagc acctaactca actgctttta agccagaacc acagaccata
    46681 tcaatcgtaa aagcaggggt atcataagaa agccccgcat ttagcgctac ttgccgagca
    46741 acattttgac ccagacctgc atgtaacaca ttcccaaaga tcacttcatc aatggcatca
    46801 cttttaagac cggtatcagc taaaattttt gtaacaactg tcacccccaa atctacggct
    46861 gataaagatt taaagactcc tccaaaattt cctatcgggg tacgataagc agatgtgatc
    46921 accacttctt tagtcataga aacttctcct ttttcattat tgaaacgctt acatttttat
    46981 tatataatgt gaactataat aaacaaatat cgttttttta tatatgctat aactaaacgt
    47041 tatcgaaaga ggaaaatatg aatatttcaa gcttggctta ttttattgag attgttaatt
    47101 gtgactttaa cttaaccaaa gcagcccaaa aaagtcatat ttcgcaaccc gcccttagta
    47161 gttatatcaa gactatcgaa aaagaagaag gagtcgtttt attccagcgt caaaaagggc
    47221 gtctagttgg actaacctca gtcggcgaac gcttttttga aaatgctaag gtggttatcg
    47281 catcacatga gcaattaatg gctgatttgc ggagtgaatc caaaaaatac aaaggagaaa
    47341 tttcaatcgg tattcctcaa ctcatctctg gcattgtttt ttctgagctc attccaaaac
    47401 tcatcgaaga aaatcccgaa gttaaatttc atattgtaga aacgggtgct tatgaactgc
    47461 aaaaaaaact gtttctaaaa gaagtcgatt ttgcgatttt attaacaccc actacactag
    47521 atgctactat tgctgatatc gacattctcc taagagatca actctgcctt tatatgagtc
    47581 acaaaacctt tcaagagacg tttgatagta atcctaacat ctctatcaaa gcattacatc
    47641 gtcaaaactt agtaactttt gataaaacct ttatgattaa tcatcagctt accaccttat
    47701 ttgaagaagc aaatagtctt cctgtagtca aatgttattc tgcctcgtgg gattttttat
    47761 taaattgcac gcgctattcc tcttacctaa caatcctacc aagacctatt actcattttg
    47821 ctcatatgga cggtttagtg gaggtccaac tgaccgaaca tccaaaatgg gaagttgttc
    47881 tagcctctct caagcacaat aaaacaagcc atttaaaaca ttatatcaag cacaccatct
    47941 tagattattt catgaaccag cgcttctaat tgcttgactc agaccttaaa aaaaacgcct
    48001 gaaggaaaca atcccaagcg tttcatgtag ttatttccta aaatacaaat aggtaaatga
    48061 tggcaatacc tgtgaccacc aagacacaag gaatagtata agccacaatt tgattgaatt
    48121 ttagtttgaa gtttgagaga taagataagg cccaaaaagg aactatcaag tttgttgtgg
    48181 catctcctaa ttgataagcc tgcaaaatct tacctaggtc attaccaact tcgatagttg
    48241 cagggacgat atagggagct tcaatcacaa attttgagcc tcctgatggt accgcaatat
    48301 tcagcaaagc cgagtaaaga taagcaaaga ccggccatgt tgtcccattt gaaatagcga
    48361 caaagaaatg ggaaatctct agtcctagac ctgtactttg aatgattcca aaaataccag
    48421 cgtaaaatgg aaactgaagc accaagcccc aagatgacat gaccccatct ttgaataagg
    48481 aaccataata ttctggtcct tgttgaccac aaagaagcaa ccctaagcct aaaaataaaa
    48541 agttaaaact attgagatct agaccagaca agcccaccac caaaaaatga taaaggctat
    48601 agctaacaat ggccccacca acaagataca tcactaagcg agatgcgttc atcttttcag
    48661 caggtgtgtt tcttgcaata acaacatgtg aagcggtatc aagactatgg tgatagatct
    48721 catcatcaag ttccatgatt ttagtttcat cttttggatg cgccaataaa gcaaagcaaa
    48781 gcggtaccag catacacacc actaaaagac tgataaatgg aagattcaaa actgattctg
    48841 tcagaggaac actttcagga acaacctgtt tataagcatc tgcgacacta tttcgcaaat
    48901 aatctggagt agccgaataa agcaccgcag caccagataa accagaagta gctggtaaaa
    48961 aggtaaaaaa taaagtagca acaaacaaag gtgtatgcac cttaatccct ttttgcctag
    49021 cctgaacaag caactctttc ccaaacacaa tggcaaccat cataccaaga ccccagtgta
    49081 aaaagccaaa gatagagcct actacaagag aaaaaataaa aatctgagtc ctggtttgag
    49141 gcagtttggc aagggactta aacatcttat gagcaggtgg agaactcgct acagcattcc
    49201 ctgtcgcaag taaaatagtc atctgcatgg caaaggacaa cagcccccaa aaaccattta
    49261 cccagccata gataatcccg cccgtctttt ccgtaccaat aaagggacta tccgtcatca
    49321 acacagacat agtaacgact aaaaaggtta aaataaaaca gataaaaagc gattctggca
    49381 tccatttcat aaagccatca atatagcgat ccataaacct ttttttggtt ttgatgttat
    49441 tcatgttgtc aattcctttc acattgtcca caagcacttt tgttcttttg atcagtaacc
    49501 tgtcgataat actaataagc gtttatttag ttcatttagt tatcaataag tttaaaccca
    49561 caaaaaacac ttttgttccc tttttattat gacaacgttt tcataaacaa acaactatga
    49621 tttttttatt gcttccataa cccttattta taacaaaaag aatggagc
//
LOCUS       DATURV010000007        45021 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DATURV010000007 DATURV010000000
VERSION     DATURV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45021
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.007"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            152..1384
                     /locus_tag="VCF75_000379"
     CDS             152..1384
                     /locus_tag="VCF75_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP2545973.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1552..2733
                     /locus_tag="VCF75_000380"
     CDS             1552..2733
                     /locus_tag="VCF75_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP2545974.1"
                     /translation="MTKRIILTIFTFICFSMMPLVHAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFTMGR
                     Q"
     gene            complement(2885..4303)
                     /gene="sufB"
                     /locus_tag="VCF75_000381"
     CDS             complement(2885..4303)
                     /gene="sufB"
                     /locus_tag="VCF75_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEP2545975.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4316..4780)
                     /locus_tag="VCF75_000382"
     CDS             complement(4316..4780)
                     /locus_tag="VCF75_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEP2545976.1"
                     /translation="MALSKLNHLYMAVVADHSKHPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHSTSRTKM"
     gene            complement(4767..5993)
                     /locus_tag="VCF75_000383"
     CDS             complement(4767..5993)
                     /locus_tag="VCF75_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP2545977.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6024..7286)
                     /gene="sufD"
                     /locus_tag="VCF75_000384"
     CDS             complement(6024..7286)
                     /gene="sufD"
                     /locus_tag="VCF75_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEP2545978.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPELELPTIERIKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKISANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVRQEIIKVLDEKLLNR"
     gene            complement(7381..8151)
                     /gene="sufC"
                     /locus_tag="VCF75_000385"
     CDS             complement(7381..8151)
                     /gene="sufC"
                     /locus_tag="VCF75_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEP2545979.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYIIPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8273..9442)
                     /locus_tag="VCF75_000386"
     CDS             complement(8273..9442)
                     /locus_tag="VCF75_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEP2545980.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9449..10210)
                     /gene="mecA"
                     /locus_tag="VCF75_000387"
     CDS             complement(9449..10210)
                     /gene="mecA"
                     /locus_tag="VCF75_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEP2545981.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10356..11195)
                     /locus_tag="VCF75_000388"
     CDS             complement(10356..11195)
                     /locus_tag="VCF75_000388"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEP2545982.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIVFIWIEKRNAQQEPAVTELARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11261..13186)
                     /locus_tag="VCF75_000389"
     CDS             complement(11261..13186)
                     /locus_tag="VCF75_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEP2545983.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRIGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGKEPV
                     DVSTSKKNKIVALETKISRRRTLLQLLFGKVIPLVEVGFLLSQLIQFTRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLILNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFVSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13384..14952
                     /locus_tag="VCF75_000390"
     CDS             13384..14952
                     /locus_tag="VCF75_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP2545984.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKIAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYAEATSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGAIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAIAS
                     FLGQMWAIFKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRYIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14945..15685
                     /locus_tag="VCF75_000391"
     CDS             14945..15685
                     /locus_tag="VCF75_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2545985.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     IAEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15827..15943
                     /locus_tag="VCF75_000392"
     CDS             15827..15943
                     /locus_tag="VCF75_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEP2545986.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16169..17179)
                     /gene="gap"
                     /locus_tag="VCF75_000393"
     CDS             complement(16169..17179)
                     /gene="gap"
                     /locus_tag="VCF75_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEP2545987.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINSAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17519..19597)
                     /gene="fusA"
                     /locus_tag="VCF75_000394"
     CDS             complement(17519..19597)
                     /gene="fusA"
                     /locus_tag="VCF75_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEP2545988.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(20081..20551)
                     /gene="rpsG"
                     /locus_tag="VCF75_000395"
     CDS             complement(20081..20551)
                     /gene="rpsG"
                     /locus_tag="VCF75_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEP2545989.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20572..20985)
                     /gene="rpsL"
                     /locus_tag="VCF75_000396"
     CDS             complement(20572..20985)
                     /gene="rpsL"
                     /locus_tag="VCF75_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEP2545990.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21193..23814)
                     /locus_tag="VCF75_000397"
     CDS             complement(21193..23814)
                     /locus_tag="VCF75_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEP2545991.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINNLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQEALAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPSLTGVKPLSYSKVETTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESR"
     gene            complement(23805..24644)
                     /gene="purR"
                     /locus_tag="VCF75_000398"
     CDS             complement(23805..24644)
                     /gene="purR"
                     /locus_tag="VCF75_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEP2545992.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(24910..25848)
                     /locus_tag="VCF75_000399"
     CDS             complement(24910..25848)
                     /locus_tag="VCF75_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEP2545993.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVEEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(25838..27109)
                     /locus_tag="VCF75_000400"
     CDS             complement(25838..27109)
                     /locus_tag="VCF75_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEP2545994.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEEINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLNASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            complement(27111..27743)
                     /locus_tag="VCF75_000401"
     CDS             complement(27111..27743)
                     /locus_tag="VCF75_000401"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEP2545995.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVPQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMLTYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(27736..28398)
                     /locus_tag="VCF75_000402"
     CDS             complement(27736..28398)
                     /locus_tag="VCF75_000402"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEP2545996.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            complement(28408..29280)
                     /gene="rsgA"
                     /locus_tag="VCF75_000403"
     CDS             complement(28408..29280)
                     /gene="rsgA"
                     /locus_tag="VCF75_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEP2545997.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(29706..30578)
                     /gene="rsmA"
                     /locus_tag="VCF75_000404"
     CDS             complement(29706..30578)
                     /gene="rsmA"
                     /locus_tag="VCF75_000404"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEP2545998.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVED
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(30692..31282)
                     /gene="rnmV"
                     /locus_tag="VCF75_000405"
     CDS             complement(30692..31282)
                     /gene="rnmV"
                     /locus_tag="VCF75_000405"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEP2545999.1"
                     /translation="MQRGYSSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHARFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            complement(31254..32045)
                     /locus_tag="VCF75_000406"
     CDS             complement(31254..32045)
                     /locus_tag="VCF75_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEP2546000.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHEAEELALAQEMGVAYHNVV
                     GFDQATISGALALADQYPHVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYHWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKETTANAKRVFKLD"
     gene            32330..33160
                     /locus_tag="VCF75_000407"
     CDS             32330..33160
                     /locus_tag="VCF75_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP2546001.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEELLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            complement(33271..34209)
                     /locus_tag="VCF75_000408"
     CDS             complement(33271..34209)
                     /locus_tag="VCF75_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP2546002.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(34231..35145)
                     /locus_tag="VCF75_000409"
     CDS             complement(34231..35145)
                     /locus_tag="VCF75_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEP2546003.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYSVADYWNAISEAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDGHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIADKDLEKAKALQYTINEIISVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLSELVEEDRVICQRAAALINQAKETF"
     gene            complement(35157..35819)
                     /locus_tag="VCF75_000410"
     CDS             complement(35157..35819)
                     /locus_tag="VCF75_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEP2546004.1"
                     /translation="MTSKKQGLLHSLFNLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSRFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELSLSTLFKRSFLLAGLFFP
                     WSFAFLALICLTIFSLQLSLLTLFGGVALLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(35863..36318)
                     /locus_tag="VCF75_000411"
     CDS             complement(35863..36318)
                     /locus_tag="VCF75_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEP2546005.1"
                     /translation="MFYDRLKNISRYRGIHPHLDAAITFLQTTDLRQLAEGKYPILGE
                     KVFAVIQRNQLSKADNSLLEYHKRYADCHLLLAGNECIRYGIGNQAEVVPFEQEADIG
                     FVTCDRTYDLDLVDDSFAYFFPNEPHQPNNFNQAGEAVTKCLIKVLMAD"
     gene            complement(36338..37168)
                     /locus_tag="VCF75_000412"
     CDS             complement(36338..37168)
                     /locus_tag="VCF75_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP2546006.1"
                     /translation="MTKKKLTASDILTTVMLCVLTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(37181..38068)
                     /locus_tag="VCF75_000413"
     CDS             complement(37181..38068)
                     /locus_tag="VCF75_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2546007.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLMNTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIVILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(38172..39491)
                     /locus_tag="VCF75_000414"
     CDS             complement(38172..39491)
                     /locus_tag="VCF75_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP2546008.1"
                     /translation="MNMKKLASLVMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTKEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAISNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            complement(39540..40244)
                     /locus_tag="VCF75_000415"
     CDS             complement(39540..40244)
                     /locus_tag="VCF75_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEP2546009.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMAEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            complement(40518..40652)
                     /gene="rpmH"
                     /locus_tag="VCF75_000416"
     CDS             complement(40518..40652)
                     /gene="rpmH"
                     /locus_tag="VCF75_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEP2546010.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            complement(40966..41880)
                     /locus_tag="VCF75_000417"
     CDS             complement(40966..41880)
                     /locus_tag="VCF75_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEP2546011.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLPRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINRQNAPAVNSADVEPEEIKATQRLEAED
                     TKVVPLMSEDSPAQIPSNLAETVTETKAQQPSIPVEESEVPQDAGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            complement(41892..42719)
                     /locus_tag="VCF75_000418"
     CDS             complement(41892..42719)
                     /locus_tag="VCF75_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP2546012.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLILMPLFNMQIKSSQKMQDIQPELRELQRKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            complement(42685..43044)
                     /gene="rnpA"
                     /locus_tag="VCF75_000419"
     CDS             complement(42685..43044)
                     /gene="rnpA"
                     /locus_tag="VCF75_000419"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEP2546013.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            complement(43584..44324)
                     /locus_tag="VCF75_000420"
     CDS             complement(43584..44324)
                     /locus_tag="VCF75_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2546014.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            complement(44328..>45021)
                     /locus_tag="VCF75_000421"
     CDS             complement(44328..>45021)
                     /locus_tag="VCF75_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEP2546015.1"
                     /translation="IWVIVKLDRFAKDQLSQKLTQAQNERIAYLENYNQSIEQLYREI
                     RTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQQSATSMMRTNYEISSLDNIKEAV
                     IRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLIDLIVLFTGLVDNAIETAKGSRRP
                     FLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQQQNSAHFLTVLDSYPQITLSTKS
                     DHYRLRQLLEMR"
BASE COUNT        13459 a         9658 c         8206 g        13698 t
ORIGIN      
        1 cttttaatga ggtttcaatt agtttaataa agattgtcat tctctcatgg tagcgtttta
       61 ttatatcatt actcttgtga ggtaagtcat taccttagac aaaaatatgg tataattaaa
      121 aaaataatac ttgagtaagg ataatctata aatgatcaaa cgattaattt ccctagtggt
      181 catcgcctta ttttttgcag caagcactgt tagcggtgaa gagtattcgg taactgctaa
      241 gcatgcgatt gccgttgacc ttgaaagtgg caaagtttta tacgaaaaag atgctaaaga
      301 agttgtccct gtcgcctcag tcagtaagct cttgacaacc tatctggttt acaaagaagt
      361 ttctaagggc aagctaaatt gggatagtcc tgtaactatt tctaactacc cttatgaact
      421 cactacaaac tatactatta gtaacgttcc tcttgataag agaaaatata ccgttaaaga
      481 acttttaagt gcgttagttg ttaataacgc caatagcccc gctattgctt tagctgaaaa
      541 aataggcgga accgaaccca aatttgttga caaaatgaaa aaacaattaa gacaatgggg
      601 catttccgat gcaaaggtcg tcaattcaac tggcttaact aaccattttt taggagctaa
      661 tacttatcct aatacagaac cagatgatga aaattgtttt tgcgccactg atttagctat
      721 tattgccagg catctcttat tagaatttcc agaagtactg aaattatcta gcaaatcctc
      781 cactattttt gatggacaaa ccatttacag ttataattac atgcttaaag gcatgccttg
      841 ttatcgagaa ggcgtggatg gtctttttgt tggttattct aaaaaagccg gtgcttcttt
      901 tgtagctact agtgtcgaaa atcaaatgag ggttattaca gtagttttaa atgctgatca
      961 aagccacgag gatgatttag ctatatttaa aacaaccaat caattgttgc agtacctttt
     1021 aattaatttt caaaaagtcc agttaattga aaataataaa ccagtaaaaa cgttatctgt
     1081 cttagacagt cctgaaaaaa ctgtcaaact tgtagcccaa aatagtttat tttttatcaa
     1141 accaatacat acaaagacca aaaataccgt ccatattact aagaaatcat ccacaatgat
     1201 cgcacctcta tcaaagggac aagtcttagg tagagcaacc cttcaagaca aacatcttat
     1261 tggacaaggt tatctggata ctcctccttc tatcaatctt atccttcaaa aaaacatttc
     1321 taaaagtttc tttttaaagg tctggtggaa ccgttttgtg aggtatgtca atacctcttt
     1381 atagaataac acaatgcttt ttgtatgaca ttgactgaca gagtacttct gcagtctttt
     1441 tcatttttat ttaagcaaac taggctaatc ctaacaattt ttcaaaaaaa cttcacattg
     1501 tattagcaaa gtgatatact gaaaatgcat tacatttaaa ggagtcggaa attgaccaaa
     1561 agaattatac taactatttt tacctttatt tgcttttcga tgatgcctct tgtccacgcc
     1621 gaagatgtca tggatattac aaggcaagca ggctacacag ttagtgaggt taatcgacca
     1681 aaatcttcta ttgtcgttga tgcaaatagt tcagatattc tttggcaaga taatattgat
     1741 attcctaggg atcctgctag tatgtctaaa atgtttacgc tttacatttt atttgaagag
     1801 ctcgctaagg gaaaaattac gatggacact actattacgg caactccaac cgatcaagct
     1861 atcgctaaca tttatgagat tagcaataat aatatcgtcg ctggagtagc ttatcctatt
     1921 cgcgatctta ttactatgac agctgtgcct tcatcaaatg cagcaactgt tatgattgcc
     1981 aattatttat caaacaatga tgcttctgcg tttatcgatc gtattaatgc cacagccaaa
     2041 caattaggca tgactaacac tcatttttca aacgctagtg gtgcagcagc acaagctttt
     2101 caaggttatt ataatcctac taaatatgat ttatctgctt caaatattac gaccgctcga
     2161 gatttgtcca agttactcta tgccttctta aaaaaatacc ctgaaattat ctcctttaca
     2221 aataaatctg ttgtgcacac tatggtagga acgccatatg aagaagaatt tcacacctat
     2281 aaccactctc tcccagacaa tcaatttggt atgaaaggag ttgatggatt aaaaacaggt
     2341 tctagtccca gtgctgcttt taacgccatg ataactgcca aaagaggcaa aactcgactc
     2401 attactattg tcatgggggt cggagattgg tcagatcaaa acggagagtt ttatagacat
     2461 cctttcgtca atgccttgac tgagaaaggg tttaaagata gtaagacctt atccaaaaaa
     2521 gcacgccaaa aactcgaaaa attagtgcca caaaccaaaa aagaaacatc acctaaacag
     2581 cagcacttca aagccactaa aaagcaatcg tacttggaaa gagtcgaaga ttttatgaat
     2641 cacaaccaca ctttcttgct tatttgctta gctatcttta taattactat ccttttacta
     2701 agcctcgttg tctttacaat gggacgccaa taatgaatta tcaaaaacct ctgtcaaagc
     2761 acagaggttt ttgatatgtt atcttacgtg tttaaggcaa ctaaaaacta accacttggg
     2821 gttagtacta caactcataa aatcctatct caaagatctt tcagatggat aagagaaaat
     2881 gcaattaacc gacagaacct tccatctcat aggaaatcaa tcgattgagt tcgaccgcgt
     2941 attccattgg caattctttg gtaaaaggct ccacaaatcc catgacaatc atctctgtgg
     3001 cttcgctttc tgataagcca cggctcatga gatagtagag ttgttcttca gaaatcttag
     3061 acactttggc ttcatgttct aacgccacct gtgaattatg aatttcgtta aatgggatcg
     3121 tatctgattt agaaatatcg tccatcaaaa tagtatcaca ttcaatatgg gaaacggatt
     3181 ttttagattg cttgttaaaa gtcacttggc cacgataatc tactttacca ccagacttgg
     3241 caattgattt tgacacaatt gatgatgatg tatgtggagc attgtgaatc attttagccc
     3301 ctgtatcttg gtgttgacct gcgttagcaa aggcaataga cagcatggtg ccacgcgcac
     3361 ctggcccatc aagataaaca gaagggtact tcatggtggt tttagctcct agattgccat
     3421 caatccattc cactgttgca tccgtaaggg cgcgtgcacg ttttgttact agattataca
     3481 cattgtctga ccagttttga atggtggtat aacgcatgta tgcaccgtca agcgcaaaga
     3541 tttcaacgat agcagcatgt aagctgttac ttgaataagt gggagctgta catccttcaa
     3601 cataatgaac acttgctcct tcatcaacaa taatcaatgt ccgctcaaat tgaccagtat
     3661 tttcattgtt aatgcggaaa taagtttgca aagggatatc taccttgacc cctttaggaa
     3721 cataaataaa ggtaccacca gaccaaactg ctgaattgag ggctgctaat ttgttgtctg
     3781 ttggcggaac cagtttggca aagtattgtt tgaacaaatc aggatattct ttgagggcgg
     3841 aatcggtatc tgtaaagata atccctagct tttcaaattc acccttcatg ttatggtaaa
     3901 ccacttctga ctcatactga gctgatgctc cagcaagata agcacgttct gcttctggaa
     3961 ttccaatacg atcaaaggtt tcttttattt tttctggaac atcatcccaa gaacgagctg
     4021 gcttatcaga tgctttttga tagtaaatga tatcatcaaa gttaatatct gataagtctg
     4081 ctccccaggt ttgcatcggc attttattaa aggtttccaa ggattttaaa cgaaactcca
     4141 acatccactc aggttcattt ttggcagctg atagttcgcg aaccactgcc tcactcaatc
     4201 cttttcctgt agaataaatg ggctctacat catcgtgaaa tccaaattgg tagtccccta
     4261 agtcaattgg ctttggctct actttctcat ttatatcaga catttttccc ttcctttaca
     4321 ttttggtccg tgaggtgctg tgctgagcat tggcacttcg tttaatggct tccttgagag
     4381 cattccaagc tagggtagag catttgatac gctgtggaaa ttttgcaact cctgccaaca
     4441 attctgcttc acctagctct ttttgagcag gattttcctg tccttgtacc atctctgaaa
     4501 aaatatcagc taacgcgagt gcttcttctt tacttttacc aataacagca tctgtcatca
     4561 tgcttgatga agctgtggaa atggtacagc cgttgcctgc aaaagcaata tcttcaattt
     4621 tatcttcgtc aaacttaacg gtcaaagaaa tcacatcacc acaagtcgga ttattcagtt
     4681 gaacagcctc tacgccatct agttgcccat gatgatgtgg atgtttcgaa tggtccgcta
     4741 ccacagccat gtatagatgg ttcagtttag agagtgccat tgaaaaactc ctttgctttt
     4801 agaattgctt cgactaaacg gtcacaatct gccttggtgt tatagatata aaaacttgct
     4861 ctaacagttg ctggtacacc taaataacta agaagaggtt gggcgcagtg gtgccctgct
     4921 cttactgcaa caccttcata gtccaaggct gttgccaagt catgaggatg caaatcatcc
     4981 agattaaaag aaatcagacc agatcttgca ctaggctggc ttggtccata tatggtaagc
     5041 ccctcaatag cttctaattt cggcaaaaca taggctatta gttctgcttc atgcgcatgt
     5101 atatcagcca tgcctagtct ctgaaggtaa gaaatggctg cgcttagccc aatagcacca
     5161 gctatgtgag gtgttcctgc ttcaaacttc cagggcaatt ctttccaagt ggcctcttgt
     5221 tcgtaaacaa aatcaatcat ttctccgcca aattcaagag gctccacttg attcaaaagc
     5281 tcttctttgc cgtaaagaac acccaaacct gttggcccca acatcttatg agctgaaaaa
     5341 gcaaagaaat cacaatccaa gtcttgaaca tcaatagcca aatgtggaac cgactgggca
     5401 ccgtcaacaa caaggtaggc tcctttagca tgtgccagct tggcaatttc tttaatggga
     5461 ttgatgcaac caagaacatt ggagacatgt actaggctaa caaaacgtgt ttttgtcgtc
     5521 aatttgtttg ccaaatcgtc catgtcaagt tggccatctt ttaaataaac atagactaac
     5581 cttgctcctg ttttttgaca ggcttgttgc caaggaatga tattggcatg atgctccata
     5641 atcgaaatca acacctcatc ttctggcgtc aagacctgct ctgcaaaccg agcaacccaa
     5701 ttcaaactgg ttgttgttcc tctggtaaag agcacttcct tgcttgattt agcatgaata
     5761 aagtcagcaa cctgctggcg actggcctca tatttgtgcg ttgcacgttc agccaaggta
     5821 tgaactcctc ggtgaacatt agcattatct tcttgataat aggattgcaa agcctcaaga
     5881 acaagcgccg gtttttgtgt ggtggcggca ttatccaaat aaacaagggg ttcatcattg
     5941 acttgttgat ttaagatttg aaagtcttgt ttgatgtctt ttgcgtctag caatactaac
     6001 ctctttcttt ttggcagtag gtcttaacga ttaagcaatt tctcatctaa aaccttaata
     6061 atctcttggc ggactgatgg aataggaatt tcagcgataa ctgctcctag gaatcctcta
     6121 ataaccaatc gttctgctgt ttcttgatcc agtcctcgac tcatcaagta atacatatct
     6181 tcagggtcaa cctgaccgat agaagctgca tgacctgctg ttacttcatt ttcatcaatt
     6241 aagaggattg gattggcatc ggctcttgct tggtcagaaa gcatcaaaac acggctttct
     6301 tgttgagcat cagctccctt agcgcctttt agaatatgac caatcccgtt aaacgttaag
     6361 gtgccacgtt ccaaaatcac accatgctgt aaaatatgac cgaccgtacg ttgaccatag
     6421 ttggtcacgc gcgtgtcaat accttgtacc tgacgaccac ttgaggctgc aacaactttc
     6481 aaatcagctt gtgagccctg accaatcaaa tcactgtcaa aatcagcaat gacattgcct
     6541 tcattcatca cagctaaggc ccaatcaatg ttggcatcct tctctaaacg tcctcgacgg
     6601 ctaatatagg ttgtcactga aggacctaag cggtcgatag ccgagaattt aatctggctg
     6661 cctgcttgag caatcacttc tacactgata ttagcgctga tcttttgagt ggcattgcca
     6721 atagattcaa aacgctctaa ataggtgaac ttactttctt ttcctgcaat cactagaaca
     6781 tgcttgttaa aaggaacgtc actgtcacta tcttgtaaga aaatagcttc aataggagtt
     6841 gtgatttcca agtgatcagg aacgtagagc acggctgcgc tattaaaata agcagtgtgg
     6901 taggcagcta gtttgtcttc atcaaaagct aatgcctgac caaaatgagc ttcaattact
     6961 tctgggattt cctcaagcgc cgtataaaaa tcactgaaaa caactccctt gtcaattaac
     7021 gccattggta actgttctaa gactgtttgc gtgcctacct gaacaagctt tgggttatct
     7081 ccaatagcta taaaatctgg aacactagct agactttcat tttctgttaa ggtaccatct
     7141 cctagattcc aacggtgaaa tttaatcctt tcgatggttg gtaactctaa ttctggtatg
     7201 gcttctaatg ccgctaaacg ccgctcttgc agccaagcag gctcagcgtg ggcttgcgaa
     7261 aaagccacta gtttttcttt tgtcatctgt caatcctttg tctctggttt ttaaattggc
     7321 tgggtatata gaacatcacc tagctttttg tagaaagcta aagtaattgt ctccaaaaac
     7381 ttaactttct tccttgtact cgattcccag atcctgtgca atacctgcgt agccttcttt
     7441 ttcaagacga gtagccaagg cagcatcacc agataagaca atacgaccat ccatcataac
     7501 atgaactagg tctgggataa tgtaattcaa caaacgttga tagtgggtga taatcatagc
     7561 tccaaagtct ttcccacgca tctcattaac ccctttagag actactttta gggcatcaat
     7621 atcaagacca gaatcaatct catctaacaa agcaaattta ggctctaaca tgagcaattg
     7681 aagaatttca ttgcgttttt tctccccacc agaaaaacct tcattgaggt aacgctctgc
     7741 catctcttct ttcataccta acaaagccat tttctcatca agtttggtga taaaatctcg
     7801 cacggaaatc ttatcctcat ctgccttacc ggcattcatt gctgcacgca taaattcagc
     7861 gttggtaatc cctggaattt ctgaagggta ttgcatggcg agaaaaagtc ccagtctcgc
     7921 acgctcatca acttctagat cgagaatatt gaccccgtca agcaaaatct gcccttgggt
     7981 cacttcgtag ttaggatttc ccataatagc tgctgaaagg gtagactttc ctgtcccatt
     8041 tggccccata atggcagcta cttccccagt tttaagagtt aggttaaccc ctttcaaaat
     8101 ctctttacct tctattgaaa catgtaaatt attaatctca agaattgaca tgatacaccc
     8161 cttattttct ctgtcatcta taccatctca agaaaagaca gtgtcatttg cactgctttt
     8221 tttataatgg tcactgcatc tattatagca aaaaaagcct tactcggctt ttttagtgtt
     8281 tcaaatcctt cttttctttc catttcagaa gcattgcttg acgataatca ctattaccaa
     8341 taaactttaa caagttaaac aaaggcgttc gcttttcacc ccagatctcc aatccttcaa
     8401 taaaaacttc taaaccaaat aaaagtccaa gcatcaagag gacgccacca attcgactag
     8461 aaacatttaa aagtaaagaa ataagggaaa aaagcatggt aataccataa actactaaga
     8521 cagctcctcg atgagtaaag cccattgata agagtcgatg atgcaggtgc atcttatccg
     8581 gctcgtagaa tttttgacct gataaactac gtcgaataat agccacgatg gtatccataa
     8641 tgggtacacc aagaataata acaggtgtca ccacagccac agccgttgaa tttttcaatc
     8701 cttgaagaga caaaacccct atcataaagc caataaagag ggctcctgtg tcacccaaat
     8761 aaatcatagc tggatggtaa ttataaggaa aaaatcctgc aatagcagaa atcaagacca
     8821 aaatggttaa cgtcaaaaag aaatcctttt gaggtaaaaa gaaataagat acaatagcca
     8881 tggtcactaa actaataata gagaccccac taaccaaacc atccaaaccg tcaatcaagt
     8941 taatagcatt agtgatggaa acaatccaca ggactgttaa gaataaggtt aagaaaggac
     9001 caaaaactaa caacggccct ccaaaaggaa ttttgaagct atcaaattta aagtcggtaa
     9061 aagcccagac aatcactgca ccaattagaa taccaaacat ctttaattta ggacttaact
     9121 catacaaatc atctaaaaaa ccagtaaggg taattactgt agcaccaatc acgacaggta
     9181 aaatataact gaagtaggtc tgaccaaaaa acatggcgcc tttagaagca atcggaataa
     9241 gtcccaaact tgctactaaa aaagacataa aaatagctaa tccaccactg gttggcatcg
     9301 gaaccttatt gacccgtctg gcattgggat tgtctactgc acccacacga aaggctaaaa
     9361 agcgaacaag aggggttaag aataaggaca ttaacaaagc ccctatcaga acaaggacat
     9421 aatctattgt gaatgaaaac atactatttt atcccaactc aatcttttgt aattgctcaa
     9481 cagcaccatc aagcatcaat tgtaagccat gctcttgtaa ataagctctt gtttttgatc
     9541 cagggtttgc atgctcaatc aatctcgcat acatgacatt cgcaaaataa gatggttgtt
     9601 gctgaacatc taataaaata gtcatatggt agcagttgct ccccttatat aactctgaag
     9661 cttctatcga aaaatcaatc gttttagcaa aagccactgc ctctgtaatc gttgaaaaat
     9721 ccaatacata atggacatag tctaaaggtt ctgactcatg attagttgat ggatcagccg
     9781 cctcagactg attagctaga gtggcttcaa caacatcttc cattatctct tcaatttttt
     9841 ccaatttttc atgagctttg acatctccct tttcacgcat ggattgctcc aagctcttga
     9901 aaaaatcttc cggagtcatt tgggacatat caccaaactc tgctaaatct tcaagattaa
     9961 tatctttatt tatctcagat ttggtaacaa aaacatctag acggtccttg cgtggagtca
    10021 ctcgaaaact aagcatacca ctatctttaa aattatctgg aagatcgagt tcgtccatca
    10081 cagaatagaa aaactcttct gttttttctt gaggaatcaa gaaatctttc aactccattc
    10141 ctctttcttc taaatcgtcc atactaattg ttatttttag cgttgtctcg ctaatttgtt
    10201 tcatttccat tctcaatacc tcgcacgcgt agtcatttta ttatactaga aatgctgtaa
    10261 tttcgcaatt tatgactaac tcctcttcta caaaaggaaa tttaaaaagc gacagtcccc
    10321 aaggtctgtc gcaacatcct aaacgatatg taagtttaaa aaacaaattt aaagaagcta
    10381 taaataagta aaaggcttcc taacacaata cggtatttcc caaaaatggt gaaatcgtgt
    10441 ttcttaacat aatctgtcaa gaacctaatc gccaataaac taaccacaaa agcggtcaag
    10501 ctagccacta aaagaataag gacttgtgca aaatcaaggt gatggccatc aaggaaaaat
    10561 ttcacagcct ttaatccact atatccaaac atggtaggaa tggccaaaaa gaaggtgaaa
    10621 tcagcggcca ctgttcgact agttcctaaa ataatagctc ctaagatggt agcccctgat
    10681 cgactagtac caggcacaat acttaagact tgaaaacaac caatgaagaa agctgttttg
    10741 taagacattc tagctaattc agtgacggca ggttcttgtt gagcatttcg tttttcaatc
    10801 cagataaaga caatcccgta gacgattaaa gcgatggcaa tcggaaccat aaaataaaaa
    10861 tgtgcttcaa accaattatc aagaggaacc gctattaaaa tagaaggaat acacgcaatc
    10921 accactttta gccataattg ccaggtaagc tgtacttctc ttgctgtttt cccaggttga
    10981 aagggattga gacgttcaaa ataaatcaac ataacagcga taatcgcccc taattgaata
    11041 acgatattaa acatttctat aaaagcctta tcctgattta gtcggataaa ttcctgcact
    11101 aaaatgaggt gaccagtgct tgaaactggt aaccattctg tgattccctc aatgatgcca
    11161 aagaaaatgg cttttagcaa ttcgataatt aacatgtttt ctccttatta accactctta
    11221 ttgtgatttt ctgaactgtg attaatgcca agcagctttt ttaaaaggca ccgccgccgc
    11281 ctcctccgcc accgccagag aagccgccac caccactaat cccgcctccg gaagagactg
    11341 agaaatttga ggaagttgta gctgaagaca agctagacac aaaggttggt gtcgtagcat
    11401 acattaccat tgataattca cctggtcgaa cagcttggta aacctcaggt aaggcaatac
    11461 gatggacttt caaatagcgt tccactttct tagcgtaacc aaataaggtg gcataaacca
    11521 aaacacggtt ccaaacaacc aaaccttcaa tagcaacatc ctcaaatttg tcaatgtctc
    11581 gtatcatgtt tttaaagctc tgccattgat gtaaccgaac acccccctca ggtgtttcaa
    11641 tacccaattt cttgtattga tctaacttca ggctaatgta aaagccaagg cacatcgtta
    11701 gcaccattaa agcaaggtaa atgagggcta acccactttc tttaatagcc aagtaaatca
    11761 ataaaccact atttagaata agcaacaaac aacctaatcc ctggacacta ttgctttttc
    11821 gtttctctgc cggggtcatt tgacgataag tatctggtaa gtttaacttt ttgatagttt
    11881 ctaggacatt gtttgtaata gctgccgacg cttttttcat cgccttaatc acctgctcag
    11941 acgattgacg gacttcttgc tcgagctttt tacctttgta cgttttcttt aattgtttaa
    12001 tggtatcagc atcataatgg tattgactaa acaattgatc caccggcaat gtcaccttgt
    12061 ttccaaaagc catatctaag aaggcagctt cctcatctgt cacacgatct aactgactga
    12121 tttcaagata agctttccct tcttctttcg ttaataaaag caccttttgg tcgatcagat
    12181 ccaacaaggt agcttgaaca agggcctcaa aggtcactcc tttagggatc aataatttct
    12241 ggctttctga tgctgtaggg gataaatagg caaaactctg tccataaatc gcttgtgtca
    12301 acacaagagg cgaaagatct tcagggactt cgtaactgtg atctgtatgg ttagccaaat
    12361 gataacgatt gaattgtttc tttaatcttg taaactgtat taactgcgaa agaagaaaac
    12421 ctacctctac cagaggaatg actttgccaa atagcagctg taacaaggtc cttcgtctgg
    12481 aaatcttagt ttctaacgct acaattttat tctttttact cgttgaaaca tcaactggct
    12541 cttttcccaa taatgctttt ttatcccaat aagcatgcaa ttccaattgc cctgagacat
    12601 tcttggcatt aatctgatag cgactgttac cttcttttaa cacttgaggt ttcttttggt
    12661 aatatcctct gtgagcccat agattagaat cctgaatatc agttggggta gtgacggtca
    12721 aactcacctt ttctaatgtt ttgtcccaat cactaatcgg tgtccaattc agctctgcca
    12781 cgtcttgata agctgttaaa aggtggtgca gttgccagat aaccttgacg tcaaccttat
    12841 caccagcttg gccagcatta taaagtttta aacgatagcc atctccaagg tcactaaact
    12901 cttgactaac aggtacctgt tgaccattct gatatacctc aactttaggc ttttggtcaa
    12961 tagcaaagcc cgctggcaaa tgacctatcc ggcccagcga aatatactgg ccgttataag
    13021 aagtgtcaaa ttgataggtt actttttgct caaaccgagc agtattttct tttgataaca
    13081 gtaactgacc ttcatagttg gtgatgctat agtctacatc agctgctttt attcgtatcc
    13141 ctaaaagact gaagcagagc acaagcgtca tcaaaatttt tttcatggca acatcctctt
    13201 tctgccctca ttttaccata ttttaaggag atgacaaaac ctaaaccaag tccttcatct
    13261 tatttttact cattattttg aatatttttc catatttcaa cctattcact gataatatat
    13321 tgaaatagga tgcaaattgc tgtaaaatag gtctgaatac tatttttgaa acaggagacc
    13381 tatatgaaaa agctaatcct tagctgtttg gtcgccttgg cccttctgtt tggaggaatg
    13441 agccgggctc aagcaaacca atatttaaga gtcggaatgg aagcagccta tgctcctttt
    13501 aactggactc aagatgacgc ttcaaacggg gctgtcccta ttgagggaac tagccaatac
    13561 gccaacggtt acgatgtcca agtcgctaaa aaaatcgcta aagctatgaa caaagaactt
    13621 ttagtcgtta agacctcttg gaccggttta attccagcat taacttctgg aaaaatcgat
    13681 atgatcgctg ctggtatgag tcctaccaaa gagcgtagaa acgaaattag cttctcaaac
    13741 agctactaca ctagccaacc tgttctagtc gtaactgcta atggcaaata tgctgaggca
    13801 acaagcctca aggatttttc tggagctaaa gtaactgccc agcaaggcgt ttggcatgtc
    13861 aatctcttaa ctcaactaaa aggtgctaag ttacaaacac caatgggaga tttctctcaa
    13921 atgcgacaag cccttacttc gggtgctatc gatgcctata tttctgaacg gcctgaagcc
    13981 atgactgctg aagctgctga tagccgtttg aaaatgatca ctcttaaaaa agggtttgct
    14041 gttgctgaat cagatgctgc tatcgctgtc ggaatgaaaa aaaatgacga tcgtatggca
    14101 actgtcaacc aagtgcttga aggattttct caaacggatc gtatggccct gatggatgat
    14161 atggttacca aacaacccgt ggaaaagaaa gccgaagatg ctatagcatc atttctaggc
    14221 caaatgtggg ctatttttaa aggtaactgg aagcaattct tacgtggaac tggaatgacc
    14281 cttctgattt ccatggtcgg aaccattaca ggtctcttta ttggattatt aatcggtatt
    14341 ttccgtacag ctcctaaagc taagcataaa gtagccgcct tgggacaaaa actctttggt
    14401 tggttactca ctatttatat cgaaatcttc cgtgggacac ctatgattgt tcaatctatg
    14461 gttatctact acggaacagc gcaagccttt ggtatttcga tcgaccgtac cctagcggct
    14521 atttttatcg tatctatcaa tacgggtgcc tatatgagtg aaattgttcg cggtggtatt
    14581 ttcgctgtcg acaaaggtca attcgaagca gcaactgctc ttggctttac tcacggacaa
    14641 accatgcgta aaatcgtgct accacaagtt gttcgcaaca ttttaccagc aacaggtaat
    14701 gagtttgtca tcaatatcaa agatacttct gttttgaatg ttatctctgt tgtggaactt
    14761 tacttctcag gtaataccgt agccacacaa acctaccaat atttccaaac ctttacgatt
    14821 atcgccatta tctactttgt tcttaccttc acggtgacac gtattcttcg ttatattgaa
    14881 cgccgtttcg atgccgatac ttacaccact ggagcaaacc aaatgcagat tgcggaggtc
    14941 tcaaatgtct aactcaatta ttgaaatcaa aaacctcaaa aaatcttacg gcagcaatga
    15001 agtactcaaa gatatctccc tttctgttaa caagggagaa gtgatttcca ttatcggttc
    15061 ttctggttct ggaaaatcaa ccctcctgcg ctcgatcaat cttttggaag agccaagtgc
    15121 cggtcaaatc ctctttcatg gtgaagatgt ccttgctgaa cactacaact tgactcacta
    15181 ccgtgaaaaa ctaggcatgg ttttccaatc ctttaacttg tttgaaaacc taaatgtatt
    15241 ggaaaatgct atcgtcgctc aaaccaccgt cctcaaaaga gaccgtgcac aagccgaaca
    15301 aatcgctaaa gaaaacctca atgcggttgg tatgacagaa caatactggc aagccaaacc
    15361 aaaacaatta tcaggggggc aaaaacaacg ggttgccatt gccagagcct tatctgtcaa
    15421 tccagaagcc atgctctttg atgaaccaac atcagctctt gatcctgaaa tggttggtga
    15481 ggtccttaaa accatgcaag atttggcaaa atcagggttg accatgatta ttgttaccca
    15541 tgaaatggaa tttgctcgtg atgtttctga ccgtatcatc ttcatggata agggattgat
    15601 tgctgaagaa ggaagtcctc aacaaatttt cgagaatcct acacaagatc gtaccaaaga
    15661 atttttacaa cgcttcttaa aataagataa aggatccaac tatttctatg ccagttgggt
    15721 cttttttcaa ttcaaaaaga atttgtcata aaatacttta ccagttaact attttatgct
    15781 atacttattt ttgttaacgt taataattta tatttaggag gataatatgg cagaaaaaac
    15841 tgacttatcg tcagcttaca gaagactgaa aagtcccaat attaaaacca gaaaacgtgc
    15901 tctgaagatt attcacgagt acaaacgtta cggtaaaaaa taagatttta ctttttatct
    15961 ctccaaattt aagtcagaca aaaaaaggct aagacacccc ttccatctta gcctttttgt
    16021 atacagttta ttaagggaac tattttaaaa tatcttttat gctagagtct agaatagttc
    16081 aaaacgttaa aatcatacat ttaaggtagt gtttaaagta tacgaaaaag aactcagcat
    16141 aaaccaagct ctctttcgtt ataactaatt atttagcaat ttttgcgaag tactcaagag
    16201 tacgtacaag ttgagcagtg taagacattt cgttgtcata ccatgataca actttaacca
    16261 attgtgatcc gtcaacttcc attactttag tttgagttgc gtcaaacaat gaaccgtatg
    16321 atacgcctac gatatctgaa gaaacgattg gatcttcagt gtaaccgaag ctatcgtttg
    16381 aagcagcttt catagcagag ttgatttcgt caacagaaac gtttttgtca agagttacaa
    16441 ccaactcagt tactgatcca gttggaacag gaacacgttg tgcagcaccg tcaagtttac
    16501 cgttaagttc tgggataaca agaccgatag ctttagcagc accagttgag ttaggaacga
    16561 tgtttgcagc accagcgcgt gcacgacgaa ggtcaccacc acggtgtggt ccgtcaagga
    16621 tcatttggtc accagtgtaa gcgtggattg tagtcataag acctttttga ataccgaatg
    16681 catcgtgaag agctttagcc ataggagcta aacagtttgt agtacatgaa gcacctgaga
    16741 taactgtttc agtaccgtca agaatgtcgt ggttagtgtt gaaaacaact gttttaacat
    16801 cgtttccacc aggagctgtg ataacaactt ttttagcacc gttagcatgt aagtgttttt
    16861 cagctgcttc ttttttagca aagaaaccag ttgcttcaag aacgatttca accccatcag
    16921 ttgcccagtc gatgttttct ggatcacgtt cagcagaaac tttgatgaag tttccgttta
    16981 cttcaaatcc accttcttta acttcaactg ttccatcaaa acgaccttga gttgtatcgt
    17041 atttcaacaa gtgtgcaagc atatttggat ctgtaaggtc attgatacga gttacttcaa
    17101 caccttcgat gttttgaata cggcggaatg caagacgtcc gatacgaccg aaaccgttaa
    17161 taccaacttt aactaccatt agtgatttcc tccttatgaa aatcaaaaga atattattta
    17221 gagggaatgc tccctaccta ttgtgaaaag attaacttac agtcatctgc aacaaccttt
    17281 caacagaact attatataac tatttcagaa aaaatgcaaa taaattagcc attttcataa
    17341 agggcagctt ctttcttcta gccttcaaaa cccaccaaat ataggaaagg gctttctttt
    17401 tctattctgt aatttatctt tttattggga aagatttcca aaacttcaaa ctgtactata
    17461 acgttctgtt acactaaaaa agaggttagg atagttatcc tcaacctctt agtggtggtt
    17521 attcaccttt gtttttctta atgatttctt cttgaactga cttaggaaca tcttcatagt
    17581 ggtcaaatac catcatgaac gtaccacgtc cttgagttgc agaacgaaga actgttgcat
    17641 aaccgaacat ttcagcaagt ggtacataag cacgaacgat ttggctgtta ccatgtgctt
    17701 ccataccatc aacacgtcca cgacgagctg tcacgtgtcc cataacgtct ccaaggttat
    17761 cttctggagc tgtaatcgtt acaagcatca tcggctcaag gatagctggt tgtgcagatt
    17821 tagctgcttc tttaagtgca agtgatgctg cgatcttgaa ggcagtttca gatgagtcga
    17881 catcgtggta tgaaccatcg taaagcttag ctttaacatc aaccattggg taacctgcaa
    17941 gaacaccgtt tgccatagat tcgatcaacc ctttttcaac agctgggata aattcacgag
    18001 gaactacacc accaacgata gcattttcga attcgaaacc tttaccttct tcatttggtg
    18061 taaattcaat ccaaacatca ccgaattgac ctttaccacc agattggcgt ttgaagaatc
    18121 cacgagcttg tgtagaagca cggaatgttt cacggtaaga tacttgagga gcaccaacgt
    18181 tagcttcaac tttgaactca cgtttcatac ggtcaacaag gacatcaagg tgaagttcac
    18241 ccataccagc gataactgtt tcaccagttt caacgtttgt ttcaacgcgg aatgttggat
    18301 cttcttcagc aagtttttga agggcaacac ccatcttgtc ttggtctgct ttagattttg
    18361 gctcaaccat caactggata actggttctg gaacttcgat tgactcaaga ataacttttg
    18421 ctttttcatc agtcaatgag tcaccagttg ttgtgtcttt caaaccaaca gcggcagcga
    18481 tgtcaccagc gtaaactgtt tcaatttctt gacggctgtt tgcgtgcatt tgaaggatac
    18541 gtccgatacg ctcacgttta ccttttgatg tgttcataac gtaagaaccg ctattaagaa
    18601 cacctgagta aacacggaag aatgtcaaac gacctacgaa tgggtcagtc atgatcttga
    18661 aggcaagagc tgcaaatggc tcttcatcag atgctggacg ttcttcttca gcatctgtat
    18721 ccgggttaac acctttgatt gcagggatgt caagtgggct tggaaggtat gcgataaccg
    18781 catcaagcat caattgaaca cctttgtttt tgaaggcaga accacaaagt actgggaaga
    18841 attcaacgtt gattgttgct ttacggatac cagcaatcaa ttcgtcgtta gtgatttctt
    18901 caccttcaag gtatttcatc atcaagtctt catcagtttc agcaactgct tcgattaatt
    18961 tttcacggta ttcttgagct tgttctaagt attcttctgg aatatcttct tcaaggatat
    19021 ctgtaccaag gtcgttggta tagatttctg ctttcatctt gatcaagtca atgataccac
    19081 ggaaatcatc ttctgcaccg attggcaatt ggattgggtg tgcatttgct tgaagacggt
    19141 catgaagggt ttgtactgag taaaggaagt cagcaccgat tttatccatt ttgttggcga
    19201 aaacgatacg tggaacaccg tattcagttg cttgacgcca aactgtttca gtttgaggct
    19261 caacacctga ttgtgagtca agaacggtta cagcaccatc aagaacacgg agagaacgtt
    19321 gtacttcgat ggtgaagtcc acgtgtcctg gtgtgtcgat gatgttaacg cggtgaccat
    19381 cccattgagc tgttgtagcg gcagaagtaa tagtgatacc acgttcttgc tcttgctcca
    19441 tccagtccat ttgtgatgcg ccttcgtgtg tttcaccgat tttatgaatt ttaccagtgt
    19501 agtaaaggat acgctctgtt gtagttgttt taccagcatc gacgtgagcc atgataccga
    19561 tattacgagt ttttgcaagt gaaaattcgc gagccatgtg tttctcctat ttttttagtt
    19621 tactctttca ttatagcact atttagcaaa aacggatagg caggacctac ccgttatcta
    19681 aatatttgtt ggtaaagata atacctattg cctttataag gcttggtatc ttattcattg
    19741 aactcaggct aaacgctgtg tgaaaaagat aaactttcct agagacaaag gtttcttcgt
    19801 caaatttcct attttcactt tgcgttctat acgccttcgt atcttattaa ttgagttcga
    19861 gctacaagcc actgacaaaa agataatagt tcctagaaac tgaagtttct gcgtcactat
    19921 tcctattttg tcttggactt gcttaacact ctcacatctt atattagttg aattttagct
    19981 acaaatgtct gccacaaaga taaaattacc tagaaactga agtttctgcg tcaatttccc
    20041 tattttggct agcatttgtt tgacgctaaa atatcttata ttaccaacgg aagtgagcaa
    20101 aggcacggtt agcttcagcc attttgtgag tgtcttcacg tttcttaact gatgcacctg
    20161 tgttgtttgc agcatccatg atttctttag caagacgatc tttcatagtg tgttcaccac
    20221 gagctcgtga tgcgtttacc aaccaacgaa gtccaagtgt tgtacgacgt tctggacgaa
    20281 cttcaactgg gacttggtag ttagaaccac cgacacggcg tgcgcgtact tcaagtacag
    20341 gcatgatgtt gtccatagct gtttcaaata cttcaagagc gtcatttcct gttgcttctt
    20401 tgatagcgct gaaagcatcg taaacgatag tagcagctgt accacgttta ccgtcaagca
    20461 taacacggtt gataagacgt gttacgattt ttgagttata taatggatct ggtaatactt
    20521 cgcgtttagg cgcttgattt ttacgactca ttttctctta tcccccttct attatccttt
    20581 tggacgtttc gcaccgtatt tagaacggcc ttgtttacgg tcagcaacac ctgcagtatc
    20641 aagtgcgcca cgaacgatat ggtaacgtac ccctggaagg tcttttacac gtccgccacg
    20701 aataagaaca acactgtgct cttgcaagtt gtgtccgata cctgggatgt aagctgtaac
    20761 ttcaataagg ttgctcaaac gtacacgagc gaatttacga agggctgagt taggtttttt
    20821 aggtgtcatt gttccaacac gagtagcaac gccacgtttt tgtggggcag ccattttagt
    20881 ttgaactttt ttgtgactgt tgtaaccaat gttcaaagct ggtgaatctg atttttcaat
    20941 tttagattta cgtggtttac gtaccaactg gttaattgta ggcatctaca ttctcctgtg
    21001 tttttttatt tttggttgat atagcacttg gtgacagccc atatccggtg cactttttgc
    21061 aacaattgtc agcacgtctc tgtacacttt tgagagacca aaagtaaaaa gtaccgtcta
    21121 atatagtaac acagacggca cttcatgtca aatagaattt ttcctttgat gcaattgctt
    21181 atgacgaatg catcacctag attccttacg gaacctaaat cctgtgaggc cgacagctgc
    21241 caacatgacg actcctgctt taagagctct ttgagtactt tcatcaacat cagaaatgag
    21301 attgctcgtt gtagaagatc ctgagccata agatgtctta gatgagggct gagtaatcga
    21361 agatgtgatt ttagacacaa tggcattact tgctaccatt tcagaggttt ggccaacaag
    21421 cgcttctgct acaagacttg tattttcagc agctaatctt gctgaagctt ctaatagttg
    21481 tttcgtttct ttaaccattt cttgaacaag cggagtagtc tctaccttac tatatgatag
    21541 cggttttacg cccgtaagag atggagctac ttgcaaatca ggcacagtag ctatatcttc
    21601 gtctaagtgg cgatattcct tttcattagc taaggtttta agcaaagtca actggtgttc
    21661 tgtggtagca atagtagctt ctagactgct ttgtttagct tgtaaggctg ctaaagcttc
    21721 ttgtgcattt aacaaagatg aggtagtttt agccaaatct tgcttagtat tatcaatgcg
    21781 ctcacgtatc acttgaaggc ggttaggatt caatttaaag tcccttaagt attgcaaatg
    21841 agctttttta gccaccagtg ctgtcactct ggctgcggct tgctctgcta aggcttctgt
    21901 ctggtgcagt gcggctttca acgatgctag cttagccaat gattgatcac gagtagcttc
    21961 gagttgtgct tgttttgctt tagctgctag taattctgtt ctcaaagaac ctttagtatc
    22021 atttaattgt ctcacttgga ggtttaagct gtctgactgc ttaagtgtgc ttgctaattt
    22081 accttgaagt tgacttactt tagcttgggc tgccataata tcagcttctt gatgaatagc
    22141 cgacaaacga ttttctaatg agcttacttt tcctttgatc gctgcaatag tatcagatac
    22201 agtgcctact ctttgggcat aatcttttgt acttccaacg gcttttatag gggtcttatt
    22261 aaagcgttga tggttagcaa tgttagactc tggaaacatt acaaagtgtt cattcaaaga
    22321 tcctacattg ctggttgaaa atccaaggta aacaggcgca ttagggttac gtttatctac
    22381 acgtaaaaag ttaatagcat ggccgtatgt atttccgtgt aaatgatctg taaagagcat
    22441 atacttgata ctgtcataaa taccacgttt aataccattc acagtatgca catcgttaaa
    22501 agcaccgata ttctcgtaca tgttatcatc atttcgaatg agccctgacg ctccggcaga
    22561 gtcttcaata atagttttat catgaggccc aacaccataa tgccctgata cccctggctg
    22621 tccgtagaca aatgatggtc ttgtattacc atgagttttc ttatagctgg tactaagtaa
    22681 tcttgcaaat tcttgtgatc ctgctgtaac agtaactggt ggtagaccta attgtcttct
    22741 tacactatta atcatgtgag ctgcaaactg cgctagctca ttttgcactt ctggtgtcaa
    22801 attatcggga tcaacaaagc gattacgatc tgctggaata tcttggtatt gatttaattg
    22861 attacctgga ctagctttgg caataatttg atctgcatgc tctttgtaat aattattgta
    22921 actagctgat ccaatataac cactagcttc taatttttta agttcttcaa gaggatagcc
    22981 ttgcggtgct ttcatggtat tattacccac aatgctatct tgagtagacg gagctgagga
    23041 tttaagacga ctaagttctg cctctttttc tgctagagca gctttctgag ctgccaactc
    23101 ttcagtcaat tgctttttaa ctttagcttt ttgattttct aagtcagctt tagccttctc
    23161 caattctgag cttaatgctt ttgtattatc attagccgtt tgagctgctt tagtgatagc
    23221 atcaggattg ctaatcatag cattgagctt agcaatattt tgttcagacg ttttgacttg
    23281 ttccactaaa tcttgagctc gagtagtttc tgctgaaatg ctagcttttt gttctgacaa
    23341 tgcagtctct tttgaatgtt gatctgcttg agcattatga agctctgttt cagtagctgt
    23401 taactctctt tgatgttcgg ctccttgggc taatagcgtc tcctcactac ttgcaagagt
    23461 attagtgtaa atttcttgtg cagaggttaa agctttttgt tcattatctt gctgctcttt
    23521 taagttgttg atttcagcag tagtttttgt cagagcggta gcaagctctg tcagttctgc
    23581 tttttgttga ctgagagttt tttcaactgc atcaatagtt gcctttgcct cttgaattgt
    23641 ttctggtttt ggtaaactat cgtcgtgagt attactcgcc ttcgtttctc ctgaggctct
    23701 atcatctgct ttgacttggt gagatacgcc tacactggca ctcaataaag caattgttga
    23761 ggttaaagca attttctgct taacttggtt tggcttcgtt tgttctaagt ccattctctc
    23821 tcctttttcc atttatcgaa aatattacca acttctacaa caacattgtt gtttttgaca
    23881 tcaatctctg aaactttcag caatgatttg aaggtcattt gctcacgctc tgattgagcg
    23941 ttttctgcaa acacagcaac accaactaaa gtgctgtcaa attctgtcaa gagactgatc
    24001 attcctgtaa tcgtcccccc gcccttcaag aaatcatcta caatcaatac acgactattg
    24061 ggtttcaaac tacgctttga cagaaacatc ttttcaatac gatcactaga agcactcgca
    24121 taattgacag acactgtcga tccttctgtt atttttaaat cacgtctaac aatgacaaaa
    24181 ggaacactta aaatattagc cactgcattt gctaaaggaa ctcctttggt tgcaactgtc
    24241 atcacagcgt caatcttttc tcctttaaag gcattggcaa taatacgacc aatattttgt
    24301 aaaattttag gtgtactcag caagtctgac aaatagatat agcctccagg taagatgcga
    24361 tcactctctg ataacctttg acacaggtct tcaacgatgg tacgcgcttc tgtttctgaa
    24421 atactggggg taaaaattac gcctccactt gctcctgtaa gtgtatcgat atcaccaatg
    24481 ttggcttctt caaaagcttt cttgataata gcgatatctt ctgagataga agattttgcg
    24541 gcctcatatt tagttgcaaa ggtgtttaaa ctagttaatt tatacggatt gttaatcaaa
    24601 taatttgaaa tgacaaccat acgttcactt cgtcttaatt tcatagtttc cccattttta
    24661 tgtacttttt gctattatac cataaagtta ataaaaaacg aacattcgat taggaaaata
    24721 ttggtttttt gttcttatta atatcatgca agaattcaat ctaagataaa ctaatcaaca
    24781 ttcctactga aaggacattc tacttaataa ccacaacaag agccaaaaac acttatgaaa
    24841 ccatcataag tgttaagcac atcaaaaatc atcacaaaag caaagtaact attacttgag
    24901 aaacactgat caatagtttg gcttgtaaaa agaccgatta tccatggcaa aaatacgatt
    24961 ggtcatctcg ccctcgctaa cacggctaag agctgttgtc atcatcatca tattagcatc
    25021 aatattgtca atcatatgaa taatttcagc ttccatgatg cgtggacgta ccggactacc
    25081 gtattctaat tgcccgtgat ggcttaaaat cacgtgccta aggacaatga cttcttcttt
    25141 ggtatcatcg atttgtagtt ctgaaattac ttttgtaatt tcttcgttaa tgagagaaat
    25201 atgcccaatc aagttaccac gaacggtgta ttctgtatta tctggtcctg ttaattctat
    25261 gactttagct aagtcatgta acataatacc tgcaaacagt agactcttat ttaagtcagg
    25321 gtagatatct ccaatgctat ctgctagtct gaccatcgta gcagtatggt aggcgaggcc
    25381 tgactcaaaa gcatggtggt ttgttttagc tgcaggataa gtataaaatt ctttatcata
    25441 cttacgataa agagccctta caattcgttg ccacgttgca ttttcaattt taaagagcat
    25501 ctgttccaaa tagtcacgta cttcttcaac actgacaggt gccttttctt tgaagtcctt
    25561 tggatcattg ggctcccctg ggcgaacatt tcgcaaggtg atttgattaa cctgaggggt
    25621 accattatag acctcgcgtc gccctttcat aaacacgact ttaccagcgg taaattcttc
    25681 aacgttataa ggttgggcat cccacagatt tcctgaaatc tcgccagtgt catcctgaaa
    25741 ggtaaggcta ataaaatctt taccagccct tgtcttacga acctctgccg acttaatgag
    25801 gtagaaccct tcaaaaagct ggtctttttt catttgatta attttcatta ttttcctctt
    25861 ctaataaggg catgttcaag agagattttg ttgcttggtc ttgataagtt tcaacggtat
    25921 tcaaggctcg aacaatggca tttgtccttg ttgaaatgag ctgatccagc gtattattag
    25981 ctgtattcat ttgcttttga gccttggcaa gcaggccgcc aaatttatcg aattctaact
    26041 tgacattgcc caaaatttta ctgatgtcat cagcattttt ttggatatta agggtcttga
    26101 aaccaacaga taaggaattg agcaaagcag acagggtcga aggacctgca accacaatat
    26161 tttcttcccg acgaaggcta tcaaagaaag ccgcatttct gaccacttct gaataaagac
    26221 cttctgttgg taagaacata actccgaaat tggtcgtctc tggggggttc aagtactttt
    26281 tatgaatgtc tttggcaaag cgtttgatag ctgccagaag tgcttttcgg ctagcctcga
    26341 tggccagttt atcaccaact tcgtaagcat cttctaatcg gtaataatct tcaagaggga
    26401 attttgagtc aatcggtagg taaatatagc cgccttgacc atttcctggg agcttaatcg
    26461 catattctac gcgttcacta gaaccactaa ccgttacaaa ttctctttcg tactggcttg
    26521 atgtcatgat atcctcaatg atttggccta gttgaagttc gcctaaaatg cctcgtgttt
    26581 tggtattgga caaaacctta tttaaagtac ccacatcttg agccacgcta cgcatttctc
    26641 ccaagccttt attgacactt tctagttgct tggatacaga atcgaaagag gcgttcagac
    26701 gattttttaa ggtttcctcc aatttttctt caacgatctg gcgcatttcc tccaaacgtt
    26761 tttcgttaga ttcttgcata tttttgagcg attggttgac ctgctggtta atttcttcta
    26821 agcgtttgtc agaccggtct ctactatcag acaaactacg gtgcaagacg tcacgaatat
    26881 ctgttaattg ttggtaaagg tctgcttgtt gtcggttcat cagctgtgtt agctctaaca
    26941 attgttgctt gttagctgta tccaactggt aggtcatttg gtcagacaaa ttatccgcgt
    27001 tgccttctag ggtttgggca agctgatgtt gaaggccgtt gactttgaac aacagataag
    27061 cccctaaacc taagagaacc aagaccaaaa ggaacaagat aaggtccata ctaactccaa
    27121 tctttacttt gaataataat caagtagcca cttggcaaac taactgtaat aggttgatgg
    27181 ataaaggcat tactggaata gattttcttt ttgaaaaaat tatcttgcgt caactcaaat
    27241 ttagcaccag taatggtcaa gtcagcttct ccttctgcca taaatgccac ataggtcatc
    27301 ccttcttctt gatggataag gtgctgacct gccgggcggt aggtcagcat attttgttgg
    27361 tcacgcaagg cgatttgtgc cataaaggga gcaataccag ggtcgctagg caaaaaaata
    27421 ttggataaga gatggtccat tcgaccccca aaagccccaa aaatggtcac ttccgcctca
    27481 ggaaaacgag caaagacctc tttaagggct aattccgtat ccgtatcatt tttttctgga
    27541 tgagcagtaa taaaaagctc tgccttctct tttatatccg taaacgcttt ttggggaacc
    27601 gaatcaaaat caccaacagc catattcaaa ggaagcccat tttctaataa aaataggctc
    27661 cctctatcaa taccgacaaa ataatcaaaa tcacggctga tataagagag atctcctccc
    27721 gcaaaaagag caactttaga cattcaaggc cgtccttaag gtttggactt ggctcactag
    27781 gtcactggcc ttgaaaaggt aagaacctgc cacaaagaca ttggcccctg cttcataaca
    27841 agcacgaatg gtcttattgt ctactccgcc atcaacctca atgtcaaagg acaatccttt
    27901 ttcatctcgc catttggcta cggtagccac tttttctaaa cattctggga taaaggcctg
    27961 acccccaaaa cctggattga ctgtcataat gaggacttgg tcaactaagt ctagcaaagg
    28021 ttctagggct gttgcaggag ttcctggatt aatcactacc cctgctttca tgccagctgc
    28081 cttgattttt tgaagggctc catgaatatg acgagtactt tctgtgtgaa tggtcatgat
    28141 atcagcacca gcttgtgcaa aagcctcaac atagcgttca ggatcaacca ccattaaatg
    28201 acaatcaaaa accaatttac tgtgctttct catacttgcc acaacatctg caccaaaact
    28261 gatgttagga acaaattgac catccataat gtcaatgtga acgtattcgg catctgtttc
    28321 ttcaatacga gctagctcag aagcaaaatt agcgtaatca gctgctagaa tagatggggc
    28381 aatttttaga gttgacatgg agtagaccta ctttcttttg ataacttttt tatatgtttc
    28441 acgacggttt tcaatttcac tgagaaattg caggtaatgc tcataacgaa ctggccacaa
    28501 ctctcccgtt tcaagcgctg ccttgacagc acacttgggt tcgtgggtat gggtacagga
    28561 tctaaactta cactcatggc tcagccggcg cagctctgga aaagcctcgt tcaaatcttc
    28621 agcattagca atatcatagt ctaatgatga gaaacctggc gtatccgcga tttttccgcc
    28681 atgggtattg taaaaactca cagctcgggt ggtgtgacga ccacgcccta gactatctga
    28741 gatttctcct gtttcaagag ccaattcggg ggcgattcgg ttgagaaggg ttgatttccc
    28801 aacacctgtt tgtcccataa agacggtaat tttatcagct agcaaaggca acaattcctc
    28861 aagactggtc acaaaatcgt aaccgattgc ctgatactgt cgaccaatag ccttaatctc
    28921 ctcgggacta tctaataagt ccatcttaga gatataaacc actggatgaa tggctttgtg
    28981 ttcaagcaag atgagaaagc ggtctagaag attgctgtta aattctggtt ctttagcaga
    29041 cataataacc actgcttggt caatattgac gataggaggt cttaccaaac tgtttttacg
    29101 aggatgaatc gctaaaatat aaccttcaga gttgtcctct gctgagaaat ccacgatatc
    29161 tccaacataa ggtgtctcac cgcgcttcct aaaattacca cgcgctcgcg tctgatatac
    29221 ctgtccttct gactccacgt aataaaaacc agccagagat ttaatgattt taccttgcaa
    29281 ctgtgctcct ttgccctact ataataactc tattatatca gaaaaccgct cccaaatcag
    29341 catttgagtc taagtttcac tcgccttaaa aaactgtcaa gattctctag accactggct
    29401 atcatttagg tcactacttt ttaaaaatag aatattttat caactaaaaa tcagatcact
    29461 gatgattttt tgaggtagga aacgatatga attttaagaa gacctttgct atttacgaaa
    29521 aagagtctaa ccgtaccgct atagtcacgc tcttgttaat tgcccctttt tttccgaggt
    29581 attaaaaaaa ctagctctgc actacgatgc cttaacggcc agcttcaata agaaaaatcc
    29641 ccctgaactg aacatctctg ttttgtctac aagggaattt ttttattgtc accgggggaa
    29701 agcttttata atccaacttc ttttaaagca tcagctaatt tgccaaaatc ttgaatcgac
    29761 aaggcctcac cacgaataga aggcttgata tctgctagtg ccagaccttt ttcaagttta
    29821 gccttgatgt cttcagactt gccaaaatgg ctagtcaagt tattccacag ggtcttgcga
    29881 cgatgaacaa aactgagacg agacactctg aaaaagaaat cttcgtcctc aacctcaatc
    29941 aaaggctggt cgcgacgcac cattttgagg atagcactat cgacatttgg tgcaggtaca
    30001 aagacggtac gcggcacaat gaaggcaacc ttggcagtca tgtaatactg taccgcaatg
    30061 gacaaggaac cataagcttt agtgtttggc tccgcagaaa tacgatctgc cacttcacgc
    30121 tgcatcatga ctacaaactc ttggaatgga attttgctct caatcaagtg catgagaata
    30181 ggcgttgtga tgtagtaagg taggttagcc accaccttga taggcaaatc aggattttta
    30241 aactgcttga tttgggtctg caaatcagct ttcaaaatat cttgattgac cacttgcaca
    30301 ttatcaaaat cgcgcaaggt atctgccaaa atcggcacca agcggtcatc aatctcaaaa
    30361 gccatcactt cagcagcatt ttctgccaaa aattccgtca aagccccaat tccaggacca
    30421 atctcgataa cattgacatt ctgatcaatc tcagccgtgt caacgatttt ctgtaaaata
    30481 ttggtatcgg tcaaaaagtt ctgaccaaaa gatttcttaa aggtaaatcc gtgacgatca
    30541 aggacagcct tggtcacgct ataatctgca attctcatat attctcttct ttttatgatc
    30601 actttctttt tggcatttga accactagcc taaacttttt tgccaggaca cgccattcag
    30661 ttaatggggc tgttatattt tattgtactc tttactcctc atatgtctcc atcacttctt
    30721 ccacttctgc tagggtaatg ccaaataatt cgaggcgctt gagcagttgt ttgccgttgg
    30781 cataaccgat gcggagcttt tcacccaagt attccctgcg tttacggcta tctgacgcca
    30841 ttaaaagccc tagacgaatc aggtctgttt gacgaatgtc aaaataagac tcatcatcat
    30901 attgctgagt gacatgagca agtgcctttt ggagatcctc aaatctggca tgttcaaccc
    30961 ctaatgaacg acccttactc ttagaactcg gaaccgcctc gtttcggttt aaaaaggcgt
    31021 gacgagctgt tggcacagct gccataatga gcttacgaat gcgctcacca ttataatctg
    31081 ggtctgtcaa aacaatgaca ccacgcaagt catttaggcg gttgatccgt tctaaatctt
    31141 cttcagtgat agcagagcct ctggtctcat aggtatccac ctcgtaaaaa cggcgaaggt
    31201 tagccgtgtc gtccttgcct tctacaacaa gaacctcttg aatattaatt ttttcagtca
    31261 agcttgaata ccctctttgc attagcagtc gtttcttttg caacttcttc cacagtcatc
    31321 ccccgtagct ctgcgatttt atcaaccaca tagcgggtat aagctgtatg attttgtttg
    31381 ccacgttttg gaactggtgt aagataaggg gcatctgttt ccaccaaaat cttatccaaa
    31441 ggcaagtgct gcgctgcctc ttgaatatca agagcctttt tgaaagtgac cacacctgaa
    31501 aaggaaatca tcatgccaag ttcaatgaaa cgttcagcca tctctaatga ccctgagtaa
    31561 gaatgcatga tgcctccacg agggccaaca ccagctgctt tgatgacttc ataggtatct
    31621 tccaaggcat cacgggtgtg caccacaaaa ggcaaatcat ggtcttttgc taattgcatt
    31681 tggcgcttaa aaacctctat ttgcacctct ttggggtcct ccatccaatg atagtctaag
    31741 ccaatttcac ccagggcaat cactttggga tgtgataatt gagagacgat agactcctca
    31801 actgcttcag aataagagcc tgcttctgtc gggtgccatc caatagtagc atacacgtga
    31861 ggatactggt ctgcgagagc aagtgctcca ctaatagtgg cctggtcaaa gccaaccaca
    31921 ttatggtagg ccacgcccat ttcttgagcc aaagccaatt cttcagcttc atgtccttga
    31981 aattctgcta cattcaggtg cgtatgggta tcaaaaatag ttagtttttc tactttattt
    32041 gtcatgatgt tattataaca taaagatctt aaccacatca gaaaacttgg tgagaacttc
    32101 tgccgtttag gttgatcgta aaaccctttg ctagctgaca cttgtctatt ttctactttt
    32161 ttgacaaatg acctttaata gtcggctcct acataagccc tcaaaaatac tacaacctac
    32221 tcaaataaag tgggttgaac aaatctgtat cacaaaaagg gtcgctacct tctcttagcg
    32281 atctatgcta tactagtaga tagttaaact gtaaggagtg tgctttgcta tgcctaaaaa
    32341 ccaccgccta attaccaaaa tcgaagcatc attggaacat atgaccagtt tagaaaaagg
    32401 gattgctcat ttttttatta caaccgatct gacacctcaa gagttgaccg catccgagat
    32461 agttaagcga ctacacatct ctcaagccgc cttgactcga tttgcaaaaa agtgtggttt
    32521 tactggttat cgtgcttttg cctttgatta ccttcacagt cttcaagaat cacaagagac
    32581 tttccaatct attcacttag aattgacaaa gcgcgtgctg atggactacg atgctcttat
    32641 taataaaacc tatgagctcg ttaatgagga agaattactg aacttagcca agctgatcga
    32701 tgcttctgag cgagtctatt tctttggcaa aggatcttct ggactagtag caagagaaat
    32761 gaagctacgc tttatgcgac ttggtttaat ctgtgatgct tattcagaca ctgatggttt
    32821 tacttgggca aatagcctcg tcaatgaaaa ttgccttgtt tttggttttt ctttatctgg
    32881 caaaacaaac tctgtcatca cagcacttca tcaagctagt cagcgagggg ccaaaactgt
    32941 cctcttaaca acagataatc aaaccgaatt tgacgactcc ttagatatta ttcccgtatc
    33001 atcaacccac caactacatt atgggaatcg tgtgtcacca caatttcctc ttcttattat
    33061 gatggacatt atttatgctt acgttttagc gattgacaaa ccgcacaaag aaaaaatctt
    33121 caaaaacact attattgaca aagaaaataa cgacctttag actacaatag caaaaagggc
    33181 gttgttttct ttttaaaggg tcgtcattca cctcccccag tatacttaaa caagaactct
    33241 tcacgtcata tagactcaca gccactaaga ttagctactc gctaaattat tttcgatttt
    33301 ctcaagagtt ataaatgaac aggatcgctc attcttttgt ttaaaatggt agtaagctcc
    33361 taatatacca gcattatttc catgactagc aaacttcagc tgagttttct tggcaagact
    33421 agaaaccaag tagctatcaa gagctgtctt taatttatca gctaaataat ctttttgcgc
    33481 catgataccg ccaccaagca ccaccacatt tgggttaaca acgtagcaga tattagcaat
    33541 accttgtcct aagtaatcca cctgcttact aatggctgct atacaaatcg catcacccgc
    33601 tttagcctgc tcaaaaatac gacgaccatc ccattggcta atgtcatcgc cataagctaa
    33661 ggccacctct tgaacaagag ctgtggtaga tgccagatcc tgaaactgac catctgacaa
    33721 atgaaggtaa ccaacttcgc aagctgaatg actactacca tgaaaaactt gggaattaaa
    33781 caataaacag ccaccgattc ccgtcccaat tgtcaagcaa agagccacag gataatcctt
    33841 ggcactacca gaaatagctt cggctagacc agcacagtta acatcgtttt ccacttcaca
    33901 cggtaacccg aaagtctctt caatctcttt tttaaactga gttccagcat agttaggaat
    33961 ctgagggccg gcataaaaga tttctccctt atcaggatta accattcctg cagaagaaat
    34021 ggccactcca gccagatcca tttggtcttg ataggtcttt accaagccct tgaccttttc
    34081 caaaatagag ggaccacctt tataagcctc agtggccatt tcctcttttt ccaacaggtc
    34141 tcctgtttca gaaatcaagc catacttgat agcagtacca ccaatatcaa ttgctaaata
    34201 atgcttcatt tgttatcccc tcgcatctta tcaaaaggtt tccttagcct gattaatcag
    34261 agcagcagcc cgttggcaaa taaccctatc ttcctcaact aactcagata atggggaacg
    34321 aacagaacca atatcaagac cctcattaat acgaagtact tccttaataa ccccatacat
    34381 attgccatga gcagaaacaa ggacactaat aatctcatta atagtatatt gaagggcctt
    34441 ggccttttct aaatccttgt cagcaataag ttggttcagc cttaaaaata attctggcat
    34501 agccccgtag gtaccaccaa taccagcagc agcccccatc aagcgccctc ctagaaattg
    34561 ttcatccggg ccattaaaaa caatatggcc gtccccacca atcgcacaga aggtttgaat
    34621 atcctgcact ggcatggatg agtttttaac cccaatcacg cgcttattag ccaacatagt
    34681 cttataaagg cttggcgtca aggcaacccc tgccagttga ggaatattat agatgataaa
    34741 atctgtgtga ggagctgcct ccgaaatagc attccaataa tcagcaactg aatattctgg
    34801 taaccggaaa taaataggcg gaatagctgc aatggcatcc acgcccaaac gttctgaatg
    34861 agctgccaat tcaatactat ctttagtgtt gttgcaggca acgtgattaa taatcgtcag
    34921 cttgccctta gcgactgcca tgacattttc taacaccaat tgacggtcaa aaacgctctg
    34981 gtaaatacac tctccagaag agccattaat gtaaagcccc tgaacccctt tgtcaatata
    35041 gtactgggtc aaggcacgca cacgctcagg gctaatagct ccttggtcat cgtaacaagc
    35101 ataaaaggca ggaataatgc cttggtattt cgtaagatct gtcatagtaa tgtcctttat
    35161 tcaatgtcat tattaagtgg gaatcgccta agaagggatt ccatgataat caagtagagg
    35221 taagttaggc tactgatgcc gatgatagct agcaaagcca cgcctccaaa taacgttaac
    35281 agagacaatt gtaggctaaa tatcgtgaga caaataagag ccaagaaagc aaaactccat
    35341 ggaaaaaaga gtccggctag taaaaagctt cgcttaaaca gcgtagatag ggaaagctct
    35401 tttctgacgg cctgaggata agcgtacaaa aagagtataa ctaccagaaa taaaacagca
    35461 atgcaaagta ctttaaaccc ttgaaaaact aggcctgatt ggtttcgaat taagaaaaga
    35521 tctaagacac aaatactcat tatcaaaagc tcaataagac ctaggcgcat cccactttta
    35581 aaataatgtt taaaacgaga gctataagta gtaacaagct gactaacttg accttctcgc
    35641 caatccaaca aactcgcata caaagccatt ttagcaaccc caattgtcag aagaggaagg
    35701 caggacaaga caaataagag attaaacacg agcaaatcaa atagtgctgc actagccctc
    35761 atccatttac tatcaagatt aaaaagagaa tgtaaaagtc cctgtttttt cgacgtcaca
    35821 ccatcacttc ctttcgatca attggttcac tgccaaatca acctaatctg ccattaacac
    35881 tttgattaag cattttgtaa ctgcttcccc tgcttggtta aaattattgg gttgatgagg
    35941 ctcattagga aaaaagtaag caaagctatc atcaaccaaa tctaggtcat aggttcgatc
    36001 acaggttaca aagccaatat cagcttcttg ttcaaaaggc actacttcag cttgattacc
    36061 tataccataa cgaatgcact cattcccagc tagcaaaagg tggcaatctg cataccgttt
    36121 gtggtattcc aacaatgaat tgtccgcttt actcaattga tttctttgaa taactgcaaa
    36181 tactttctcc cctaaaatag gatatttccc ttcagctagt tgcctcaaat ctgtggtttg
    36241 taaaaaggta atcgccgcat ccaaatgagg gtgaatccct cggtaacggc taatattttt
    36301 taaacgatca taaaacatgt tagaaacctt tcttgattta acctttcaca gcacccatag
    36361 taatgccttg ggtaaatgat ttttggaaga caagaaagac tgttacaata ggcactgcag
    36421 ccatggcagc ccctgccata atcaagccat aattagtagc catttcagct tgcatagtgg
    36481 caaccccaag tgaaatcgtt aaattttccc gagaggttaa catcactaac tgcataaaat
    36541 aatcattcca agtattgata aaggtaaaaa tcgctagcgc tgcaaagcct ggtttgacaa
    36601 tagggaaagc cacattgaaa aaggtacgaa tttcgccaca tccatcaatc ttggcggatt
    36661 ccaataactc ggttggaata ttttctgaga actgtttcat aaggaaaaca ccaaatggcc
    36721 agcccacaag aggcaaaata acagccgcca aagtatcatg gattcccata aaattaacaa
    36781 tccgaacaag aggtactagg acaacctgtt ttggcaaagc catagcagca ataaaaatcg
    36841 aaaacaaaag ccgctgccca taaaaccgtt ttttggccaa agcatagcca gccaaagagg
    36901 aagtgccaca aaccaagaac atggtcgcca ccgaaataaa gacactattc cacaaccatt
    36961 ttaaggctgg attttgcact accaaggcct taaaattttc aatagtaggc gcctttggcc
    37021 accattgcgg tggaatcata atggtatcgg cttgagcctt aaatgctcct gtcataatcc
    37081 aataaaatgg aaaaataaac aaaatggtca aaacacatag catcacagtg gttaagatat
    37141 ctgatgcggt tagtttcttt ttcgtcatct taatatcctc ctaatattcc acatcattac
    37201 ctaaaatttt aaattgagca aagctgatga ttgcaatcat gactgccaag aatacaccca
    37261 ttgtgttggc atagccatat tcagagagct taaaggcctt ttcatacaaa taatacatga
    37321 gggtactggt tgaataattt ggccctccag acgtcaagag ttgaatcaaa gcaaaacatt
    37381 ggaaggaatt aatggttgtt atgacagcaa tatataaggt tgttggcagg agacttggcc
    37441 acttgatatt ccaaaacacc tgaaattccg tcgccccatc cacacgcgca gcttctacca
    37501 aagagttatc aatatttccc atagctgcaa tatatagaat aataggctgc ccaacagatg
    37561 tggttaataa aataacaata atagctaaca aagcccagtg cttatctccc agccaactaa
    37621 tgttttgttc aatcacatga gcatatttta agacataatt caaaatccct gacatcggat
    37681 catagatcca tttccaaacc accgtcaccg caacacttcc ggtaacaaca ggcagaaaga
    37741 aaactgcccg gtaaaacgaa cgcgccacta catttttgtc gtaggtttta gccgccacaa
    37801 agagggaaaa gaaaactaca acaggtaccg aaccaataac aataatcaag gtgttcataa
    37861 gagacttcat gaaaatggga tcttgaaaca tcctagcata attagcaaaa ccaacaaagg
    37921 taaaatccgt catggaataa ttgaaaaaac tcgtcacaaa gcccataatc atcggtatca
    37981 agacaaaaat cacaaagaaa accaagaccg gagctaagaa agcgtatgag atgagcgtct
    38041 ctctcatttt taatttattg acgttcactt gaaatacctc tttttctatt aatctctcct
    38101 aaaaaaggaa aggctccttt ccagtgacca tttgacatgt gccgtgaaaa gaagccactt
    38161 gttctgaaca cttattgtgt ttttttgatt gtcttgtttg ctttttcagt gaaggctttc
    38221 aaagcatctt ctggcttttc atcaccatta gagatggctt gaaccattgg gaaccaaaga
    38281 gttctcattt cagcaaaccc atcaatcgtg ttgtagtatg gtgagtagaa ttttgtccat
    38341 tcagcaattt tttccattcg tttgtctttg taaagatccc cataagaagt tcttacaggg
    38401 aaagcacctg tacgaacaac atttttaggt ccccattctt tatcatcagc gataaattgg
    38461 ataaatgtct tagaggcagc gactttttgt tcatctttgt tattaaagac cgcaaaacca
    38521 tttacgaggt attctagttc tggtttgcca tcatctgatg ggaatgggat ttcaaggtaa
    38581 tccactttac tagcttctaa caatttagct tggatgccgg gttgtgcagg cgcccaaaga
    38641 atggtaaagg aagtttggcc attcgcaaag ttttgaatat cagctgaacc gtcgtattga
    38701 gagccattca tcattaaacc atctttaatc caattggaaa ttttagtcat ggctttaata
    38761 gagttagcat catctgtggt gtatttggtt actttgtcat cggtaatatg actactataa
    38821 aggttagcaa agaaggcacg tggtccttgg tcaccacctt gaccattagc aaagaaagaa
    38881 cctgggttat atcctttgtc cttcaatgct ttcaagactt tttcaaaatc atctgttgtc
    38941 cacccctctt tcacaaggtc aaggacgcca gcatctttga gcattttttt attcaacgcc
    39001 atgtagaatg gagcagagct gataggatac atgtaagctg tatctcctgc ttttgaggct
    39061 tgaatcaatt tgtcattgtt aacatctttg gtaaactctt ttgtgaacaa gtcattcaag
    39121 tcagccaatt tcccattttt accatattga atgatacgac ctggagcatc aaagaggaca
    39181 tcaggagctg ttccagcttc aatagcagtt gtgattttct caggaccaga tgtaaaatca
    39241 atggtttcca attttacctt gatttctgga ttagctttct caaaggcagc aatcaatttc
    39301 ttctcatagg ttcccacacc atcttcagct ttttcttgcg tgaagaccgg gaaagcccac
    39361 catgtgattt ctgtcttagc agtatcagat ttacttgcac cagcctcttt ctggctcttt
    39421 ccgccacaag ctgctagtcc taaaacagat gcaccaagca tcactaatga agctaacttc
    39481 ttcatgttca tcgtaaaatc tccttttttt gatgtgtgtt tctagtttga caaccactat
    39541 taggatttga gcgcttcgat aaaacgctct gcaatttcct taggccttgt aatggcccct
    39601 ccaacaacaa tacctgccac acctaagtca ttaattttct tggcttcttc tggggaatga
    39661 atctttcctt cggcaatgac agcaatgcct gctttgcaaa gagcttctat caatgccaca
    39721 tcaggacctg cttcctgacg gctatacggg gtgtagcctg acaaggtggt tccaacaaaa
    39781 tcaatgccag cctgatgggc cactagtcct tcatcaaagg tactaatatc tgccatcaaa
    39841 agctgattgg ggtacttttc cttaacttgt cgaataaagc tagcaatatc caagccatca
    39901 tgtcggtcac gcttggtaca atccatagca atcactgcaa tatttaaggc agctaattga
    39961 tccacttcgg ccatcgttgc cgtaataaaa ggctcttgcg gcgggtaatc ttttttgatg
    40021 ataccgataa ttggtaggtc tgttatggct tgaatttctt taatgtctct aaccgaatta
    40081 gctctgatgc cgacagcacc agcttcttga gcagcttttg ccagcagtgg cataataccc
    40141 ccagtctctg aatagagagg ctcacctggc aaagcctgac aagaaacgat aatcccaccc
    40201 tttaactgct ccatgagctt ttctttggtt ggtttatcag gcatagtttc tactccttgt
    40261 gtttcttagt tttaataaaa ataacgttca caaagttatt ataacaaaaa ataaagcgct
    40321 ttcaatagca atgagacttt tagaatctac tttcattttt tatattattt tctattattt
    40381 ggaatgctct ttcatacaac aaataaaaag gtaaggagtt gacaccatac ttatacattg
    40441 caaatttccg attagaaaac gttaaaaaac caagagcctc gagcactctt ggtttacttt
    40501 agttgtattt tttgcaatta agctgataat actttacgtc ctttacgacg acgtgcagca
    40561 agcacgcgac gtccgttttt agttgacata cggtgacgga atccgtgttt acgttgacga
    40621 cggattttac ttggttgata agtacgtttc acagtgaata cctcctcata gattttgtat
    40681 tcgtttagcc ggctatttgt gatatcagtt actaaacata ctataatatt ctatcttaaa
    40741 gagggtctac tgtcaagatt tattttcgtt tttcaacagt ttctctctca tttaagaggc
    40801 cgctcatttc ataaagtgtc tcttctttga cactgttcct ttgccttacc gttgaaacct
    40861 tatcaatagc aacaataatc aatgagatca gtaaaacaaa cggttaagct ggccatacaa
    40921 aaaatcttcg atagctaaac taactaccaa agattctttt aggatctaac gttgaagact
    40981 gactaccaca tagcggttag ggtcattgcc ttcagaataa ctatcaaccc cttcaatgga
    41041 tgaaacggtt ttatgaacga ttttacgctc actgttactc atgggatcca tggtgtaatc
    41101 ttgaccagat tccaaaacgc gtttggcaac tttttgcgta aagtcaatca aggtttctgt
    41161 tcgatgttcc acataatcat ggacattcaa ggatactgaa aaattttttg aatagcggtc
    41221 gtgcaagaaa ttttgagcta acaactgcaa ggatttcaag accttaccat ggtaaccaat
    41281 cacacgtccc gcctctggcg tttcaatctg caaattgatt tggcgacgat tgttgctggt
    41341 ttcaacggta gcttcgatat ccatttcata gataatttta gtcacataat cggacacttc
    41401 ctgagcagct ttttcaatat ctttgctgaa accatcatta ccagcatctt ggggcacttc
    41461 tgattcttcg actgggattg acggttgttg tgcttttgtt tcagtgactg tctcagcaag
    41521 attagaaggg atttgcgctg gactatcttc agacatgaga ggaaccactt tggtatcttc
    41581 tgcttctaac ctttgagtgg cttttatttc ttcaggttct acatcagcag aattgaccgc
    41641 gggagcattt tgacgattaa tatcctctgg aacacctcga gtggcttttt tatcagcctt
    41701 ataaaccgtc ttgtcgctga taccttcgat atcaacttgg gctggctttt taccaaaccc
    41761 gaggaaacct tttttctctt ttgaaatgac tttgatatgc gctttaaggc gtggtaaccc
    41821 taactcttga agtcctgttt cgatagcttc ttcgactgtt tttcctgtaa ataataccat
    41881 aacctaaact cctattttct cttcattgct tttttacgag cacgacgttc acgaagacgg
    41941 cgttcttttt cttcattggc aagacgttgt ctttccgcaa taatcttaaa aggattgttt
    42001 aataacagta gttgtaccac ttgaaaggca ttggagaccg tccaatagag cactactcca
    42061 ctagccaagt taaagcccat gaaaaagatc attagcggca tcacataaat cataacagtc
    42121 atcatgacat ttttttcttt agccgctaga ttggtcaacc aagtggacaa gaaagtgaag
    42181 acagctgcca aaactggtaa aaggtacaaa tgatcatgtt gtgccaattc cacccacaag
    42241 aaagtccctg tttttaagaa agatacccgt gtcaaggctt ggaatagggc aatcataact
    42301 ggcatctgaa tcaagagggg taagagacta gcataagggt taaccccata ttttttgtaa
    42361 agggcttgac tttcttcagc caacttcatc cgcgtttgtg tgtctttacc agcgtatttc
    42421 ctttgcaatt ctctaagctc aggttggatg tcttgcatct tctggcttga tttgatttgc
    42481 atgttaaaca aaggcatgag tatgagacgg atagtaaggg taaaaagaat aatgccaaca
    42541 ccaattgaac catcaaagga aagccattga attgctctgg caaataagta aaccaactgg
    42601 tcccaaccac tagatgattg tgccgttacc tcaccacgac cacaggccac taataataga
    42661 actaagggga ctattcttgc aattttaatg tttttcttca ctctcaaaac ctttctcaag
    42721 caattgtgct aactttaaaa catgatgtaa attttgttga agctcttgat actccaaaga
    42781 ctcgacaccc ttacgggcaa tcaccacgaa atcctctgac ttcagctgat gccctaatgc
    42841 catgataaca tgacgtatct ttcgtttgac tgcatttctg gtgactgcat ttcctatttt
    42901 tttaccgaca gaaataccca cacggaagtg gtcttggcct ctatttaaat gataaatgac
    42961 aaattttcga tttgctgtac tttttccatc cttaaatatg gcttggaaat ctttctcacg
    43021 cttgacacga taggtcttct tcaaaattta actccaatat ctaaattatt accattatac
    43081 cacatcccct aaaaaattaa aaagccctaa ccctttcaga ttaagcctct ttctgtacta
    43141 tttctagttt ttgtgcttca actgcctctt gtcactccat cttttcgtag tatagttctt
    43201 ctttcgacaa aggaataacc ttcagaaaac aaccataaaa agtcagaatc cacaagacgt
    43261 cagctactta tccctgtatt tacctatcaa aaaacccggc aagccgggct ttaatactga
    43321 ttttacaatc agctaatatg atatccaaac aaagacaact gacatcgtca ttttgtctgc
    43381 cgagccatca tatcaaaaat actatttgtt tatggagaga gagaaaacaa tattttgatt
    43441 gaattgggga ttgattgctc gataaactca tgacttcgta aatctttatt tacattaatt
    43501 attatagcga aaaatacttc aataagagca tcatttcctg aaatgttgct ttttcgtcct
    43561 aaaatgttat tcctctctta actttagcga agggcctccc accgttcaaa cagacatttc
    43621 atttttaacc gtgataccat acaagaatca ccattttcga aataaaggag ctgcttactc
    43681 ttatcaattc gaacaacctt atctagattc acaaggtagg atctatggca caaaaaaagt
    43741 ttcggtgcta ctgcttgaat ctcagataga ttaccataaa actctaatct ttctgtctga
    43801 gtccataaac aaactttgtg aggagttgtg gctgtagcaa agtataaaat atccttatag
    43861 ggcaatttta atcttgtctg tggagtctca aataaaaaca tgtcctttga ctcacgacta
    43921 gacatcatat cgtaagtata tcggatacat tcttcgattt gttctttaaa ctgtttttga
    43981 ccacctgctt tatcaataaa atctagagcg gataccttat atttaaaaga aataggagca
    44041 aattctgaat gagtggtgac aaacacaata acggcattag gatctttttg tcgaatagct
    44101 gcagctaatt caagcccaca acgagtatat tcaccaattt caatatctaa aaaatacagc
    44161 tgatggtctc ctctttcttg gatactctca aataattttt gaggagtcga gaacacttca
    44221 agctgattac atggtattct agtctctttt aagatgccta ctacgataga ttctatgcgt
    44281 gtctgctgga taaaatcatc ctcaagaata aaaatgttca tcatacttta tctcatttct
    44341 aacaattgtc ttaatcgata atggtcactt ttagtcgaaa gcgttatttg ggggtaacta
    44401 tctaaaacag tcaaaaagtg ggctgagttc tgctgctgag catcaaagcg tttggctata
    44461 tccacacgat tggtttttgt gctattttca ataataaata attgcttgtt atcttgttta
    44521 aagtatgcaa tagataaaaa aggacgtcta ctgcctttgg ctgtctcaat agcattgtcc
    44581 actagtccag taaaaagtac aattaaatcg attaacttaa taggtaaatg atcgataaca
    44641 tctggaatct caatataaag ttcaataccc aaatactgag cttcaagcaa tttggaattc
    44701 atgatgctgc gaatcacagc ctctttgata ttgtctaaag aagatatttc ataatttgtg
    44761 cgcatcatag aagttgccga ttgctgaata accgtgtcat aaactctagt aatcagatct
    44821 atatcaccac tatcgatact atcttttaaa ctaattaaaa tattttcaga atcatgttta
    44881 actgttctga tttctcggta aagttgctca atcgattgat tgtaattttc aagatacgca
    44941 atacgttcat tttgtgcttg tgttaatttt tgtgacagct gatctttagc aaatcgatct
    45001 aatttcacaa taacccaaat a
//
LOCUS       DATURV010000008        39919 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DATURV010000008 DATURV010000000
VERSION     DATURV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39919
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.008"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(178..2976)
                     /gene="ppc"
                     /locus_tag="VCF75_000422"
     CDS             complement(178..2976)
                     /gene="ppc"
                     /locus_tag="VCF75_000422"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEP2546016.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQTIIAEEVALLKEMLENITRR
                     MIGDDAFTVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINHQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMNSSVGLNTSAWPSPASLEKPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            3135..4934
                     /gene="pepF"
                     /locus_tag="VCF75_000423"
     CDS             3135..4934
                     /gene="pepF"
                     /locus_tag="VCF75_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP2546017.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEESLQDIDLFKRN
                     YEDRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMDDFEVDGKTYKNSFVSYENFYQNHENAEIRDKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYTSVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVMSTAGYLNLKNNPDGAKEWLDFLKSGGSRTPFDTAMLIG
                     ADIATEKPLRDTIQFLSNTVDQIINYTEEMSHA"
     gene            4927..5406
                     /locus_tag="VCF75_000424"
     CDS             4927..5406
                     /locus_tag="VCF75_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEP2546018.1"
                     /translation="MPNLYDFTVKAQNGNDLSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFAWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(5452..5838)
                     /locus_tag="VCF75_000425"
     CDS             complement(5452..5838)
                     /locus_tag="VCF75_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEP2546019.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQIQTTPSVPKADHHVRLVV
                     KEDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNG
                     KMADKGVDQITVKDGDSIEFYQEVFK"
     gene            complement(5822..6325)
                     /locus_tag="VCF75_000426"
     CDS             complement(5822..6325)
                     /locus_tag="VCF75_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2546020.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFVLYPLSQQFPKYQLGCQ
                     AFLVALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            6650..7498
                     /locus_tag="VCF75_000427"
     CDS             6650..7498
                     /locus_tag="VCF75_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEP2546021.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKASTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFTTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     regulatory      7625..7721
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7766..8329
                     /gene="thiT"
                     /locus_tag="VCF75_000428"
     CDS             7766..8329
                     /gene="thiT"
                     /locus_tag="VCF75_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEP2546022.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     gene            8420..8899
                     /locus_tag="VCF75_000429"
     CDS             8420..8899
                     /locus_tag="VCF75_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEP2546023.1"
                     /translation="MAKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            9240..9863
                     /locus_tag="VCF75_000430"
     CDS             9240..9863
                     /locus_tag="VCF75_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP2546024.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNTSLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(9971..10873)
                     /locus_tag="VCF75_000431"
     CDS             complement(9971..10873)
                     /locus_tag="VCF75_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP2546025.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DAAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            11047..12540
                     /gene="lysS"
                     /locus_tag="VCF75_000432"
     CDS             11047..12540
                     /gene="lysS"
                     /locus_tag="VCF75_000432"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEP2546026.1"
                     /translation="MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKYFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            12676..12816
                     /locus_tag="VCF75_000433"
     CDS             12676..12816
                     /locus_tag="VCF75_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546027.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(12913..13125)
                     /locus_tag="VCF75_000434"
     CDS             complement(12913..13125)
                     /locus_tag="VCF75_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEP2546028.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(13337..14623)
                     /locus_tag="VCF75_000435"
     CDS             complement(13337..14623)
                     /locus_tag="VCF75_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP2546029.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            complement(14725..15651)
                     /locus_tag="VCF75_000436"
     CDS             complement(14725..15651)
                     /locus_tag="VCF75_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP2546030.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            15850..16134
                     /locus_tag="VCF75_000437"
     CDS             15850..16134
                     /locus_tag="VCF75_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEP2546031.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLLAKVIKSLLM"
     gene            complement(16294..16722)
                     /locus_tag="VCF75_000438"
     CDS             complement(16294..16722)
                     /locus_tag="VCF75_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEP2546032.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEDDIIIDYTEQDEPVSDTLDKH"
     gene            complement(16715..17122)
                     /locus_tag="VCF75_000439"
     CDS             complement(16715..17122)
                     /locus_tag="VCF75_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEP2546033.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            complement(17144..17923)
                     /locus_tag="VCF75_000440"
     CDS             complement(17144..17923)
                     /locus_tag="VCF75_000440"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEP2546034.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            complement(17920..18852)
                     /locus_tag="VCF75_000441"
     CDS             complement(17920..18852)
                     /locus_tag="VCF75_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEP2546035.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            complement(19217..19489)
                     /locus_tag="VCF75_000442"
     CDS             complement(19217..19489)
                     /locus_tag="VCF75_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEP2546036.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            complement(19553..19990)
                     /locus_tag="VCF75_000443"
     CDS             complement(19553..19990)
                     /locus_tag="VCF75_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEP2546037.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(19977..22109)
                     /locus_tag="VCF75_000444"
     CDS             complement(19977..22109)
                     /locus_tag="VCF75_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEP2546038.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            22343..22498
                     /locus_tag="VCF75_000445"
     CDS             22343..22498
                     /locus_tag="VCF75_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546039.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            22646..23548
                     /locus_tag="VCF75_000446"
     CDS             22646..23548
                     /locus_tag="VCF75_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEP2546040.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            23548..24363
                     /locus_tag="VCF75_000447"
     CDS             23548..24363
                     /locus_tag="VCF75_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEP2546041.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMILSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFVDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(24462..25886)
                     /locus_tag="VCF75_000448"
     CDS             complement(24462..25886)
                     /locus_tag="VCF75_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP2546042.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADAKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     GKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(25905..27767)
                     /locus_tag="VCF75_000449"
     CDS             complement(25905..27767)
                     /locus_tag="VCF75_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP2546043.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLALFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFTNALIAGAATIVLAFSLTWFMGIDEE
                     SPKQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            complement(27769..28611)
                     /locus_tag="VCF75_000450"
     CDS             complement(27769..28611)
                     /locus_tag="VCF75_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP2546044.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLIVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            28939..30306
                     /locus_tag="VCF75_000451"
     CDS             28939..30306
                     /locus_tag="VCF75_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2546045.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(30360..31910)
                     /gene="ftsY"
                     /locus_tag="VCF75_000452"
     CDS             complement(30360..31910)
                     /gene="ftsY"
                     /locus_tag="VCF75_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEP2546046.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEVVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHSSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATYDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KEVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(31910..32734)
                     /locus_tag="VCF75_000453"
     CDS             complement(31910..32734)
                     /locus_tag="VCF75_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2546047.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLINDESGVAEAVKTFILEEES"
     gene            complement(32738..33535)
                     /locus_tag="VCF75_000454"
     CDS             complement(32738..33535)
                     /locus_tag="VCF75_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2546048.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            complement(33673..33987)
                     /locus_tag="VCF75_000455"
     CDS             complement(33673..33987)
                     /locus_tag="VCF75_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546049.1"
                     /translation="MTRLISQQILSEYVKSLEVEADKVVIPGQNTPVFSWDRDGNGNL
                     SIDTPLLKVRGESLATQADLTTISLTPGPKGELWQTSVERLDIKVDKHISLTFKNGVR
                     ID"
     gene            complement(34053..34505)
                     /locus_tag="VCF75_000456"
     CDS             complement(34053..34505)
                     /locus_tag="VCF75_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP2546050.1"
                     /translation="MGLLDLIGRKRARDKPQNSYEGQDFSYLFGRTISGENVDEFKTM
                     QTTAVYACVRILAEAVASLPIHIYERTENGKEKKLDHPLYFLLHDEPNPEMSSFIFRE
                     TIMSHLLIWGNAYVQIIRDKSGMVSLLLKQKASVLIRKLLLIIWQRMS"
     gene            complement(34610..34830)
                     /locus_tag="VCF75_000457"
                     /pseudo
     CDS             complement(34610..34830)
                     /locus_tag="VCF75_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34849..35154)
                     /locus_tag="VCF75_000458"
     CDS             complement(34849..35154)
                     /locus_tag="VCF75_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP2546051.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQGM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(35154..35456)
                     /locus_tag="VCF75_000459"
     CDS             complement(35154..35456)
                     /locus_tag="VCF75_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEP2546052.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLITSFEDAKTGNRQDVREFLSQLKENA"
     gene            35502..36596
                     /locus_tag="VCF75_000460"
     CDS             35502..36596
                     /locus_tag="VCF75_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546053.1"
                     /translation="MTQLKTQYPSLIIDASFITLAANATVKPQDKTYNTVNLLNRFFL
                     IWLDVASRGLSHHHVLSHPFKDWMTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQA
                     DTAIPRYLLVTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCD
                     RPLILTSGNLAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVK
                     KILVSAHNSQQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTL
                     YQLVKNPVIDNYLTIQKIIVNLDKQGRIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTI
                     SEKLHKATLQDIYFCQMIVSYFIQKCEVLE"
     gene            36596..36820
                     /locus_tag="VCF75_000461"
     CDS             36596..36820
                     /locus_tag="VCF75_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546054.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            36811..38469
                     /locus_tag="VCF75_000462"
     CDS             36811..38469
                     /locus_tag="VCF75_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEP2546055.1"
                     /translation="MLIEMWSPVFKNHGEIRESICFHYGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSRDTANPNIILVCDKSYSP
                     TGETMDLGIFLNRLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTHQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETILDSEFSTERESLIAEIDEL
                     ESQLTTLNQELQELGNIPNLSSEYLESYSKLTATINALKEQNEAYLKESGLSKEKSEA
                     DADLKRSTEDILLDLEVKINSKMREFNNILYPDIRKAPQINLNAHNSYSFYTPDDDGT
                     GTKFKGMIVFDLVMLYLTNLPALTHDSLLLNNVSYQATEALLKLYDQSRSLNKQVFLA
                     FDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            38459..38668
                     /locus_tag="VCF75_000463"
     CDS             38459..38668
                     /locus_tag="VCF75_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2546056.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
     gene            complement(38770..39909)
                     /locus_tag="VCF75_000464"
     CDS             complement(38770..39909)
                     /locus_tag="VCF75_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2546057.1"
                     /translation="MFDFANNTFLAGLNPASLSLVAVYQSLESVIGVLFNLFGGVIAD
                     SFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMSAFSGPSYKAFTK
                     EIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLDGLSFLIAALLIS
                     FILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSALVNFFLAAYNLLL
                     PYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLILFLSLSGLMLML
                     APPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLGRVFGIIFTITIL
                     FMPIGTGFFSVALNPNNSFNLFIIGSCITILSLVFRILFKKYNIR"
BASE COUNT        12117 a         8472 c         7104 g        12226 t
ORIGIN      
        1 atcttcatag ttttccctct acttcttgag ataatcattt agcattataa catctttcta
       61 tatcaaacaa aaggaaaagt caagaatcct caaaaaaaga agctccaagg gctaaccttt
      121 aaaattctga ttagtcactt gaaaacttct ataaatacca attaatactc aagagtatta
      181 gcctgaatta cgtaaaccag ttgctatgcc attgatagtt gtatggatca atttttcatc
      241 attttcgtca agttgattat tacgccaacg tttaatcaat tcaatttgaa tatagttcaa
      301 cacattaaaa tacggcaagc gtgacttcaa gctatgctta agggaaggat tatcctccaa
      361 tagatcatca tggtcttgga tagcaagaat aacatttttt gtcaattgcc actcatccaa
      421 aatctcataa aaaacatctc gaacatcctg actttcggct agttgtgcat actgaaaggc
      481 aatattcatg tttgatttag ataataccat atcaacattt gataacaagg aatggaaaaa
      541 aggccacgtc tgatacatgt gttggaggcg ttcaaggtta ccttgtgctc ggtcaatata
      601 acgtttaaag gctgaaccta ccccatacca acctgggaac atgatgcgat tttgtgacca
      661 tgaaaagacc caaggaatgg cacgcaagcc tgtaatttcc gtaatcgttt tacgggctgc
      721 tggtcttgac ccaatgttga gactggaaac ttccttaatc gggcttgcct caaagaaata
      781 atcatagaaa tggggattgt caaaaaccaa ttttcgataa atggtgttgc tatcagcaac
      841 aatgctatcc atgatttctc gaaaactatc aatctcatta ggatctgtta tcatttgagt
      901 caccatacga ttgatagaag ctgaaatcaa catttctaaa tggtaataag ccacatcttt
      961 attgccgtat ttgttttcaa taatttcccc ttgctctgtc agacgaatcc gatctttgat
     1021 ggaaccaaaa ggttgtgaag taatggcatc ataagaaggg ccacctcctc gaccaactgt
     1081 tcctccacga ccatggaaga aggtgatttt cactccatgt tcctcaccaa tagccgttaa
     1141 ttcattctga gctttataaa gagtccaacc cgatgccaag tagccaccat ctttattact
     1201 gtcagaataa cctaacatga tttcttggta attacgattc gttgcaatcc aagatttgac
     1261 aatgtcatgg cttaaatagg ctgccatgat gtcacgggca ttatctaaat cctcaatggt
     1321 ttcaaagagg ggaacaattt gcacacgagc ccgctgctgg tcaaccaatc ccacttcttt
     1381 aagcatgatg gccaactcaa acatatcaga gacactttct gtatgtgaaa taatatgttg
     1441 cttaataacc tcttctccaa gcttatcttt caattctcgt gccgtagtat agatagccag
     1501 ttctttttct aataggtctg atttagcaac tgccgctgag gataaggttc ttggctcctc
     1561 cattaattgt tgtaagagaa catcgcattt ttcagtttct gaaagagagc tataatcgtc
     1621 aacaatattg gctcctttta ataactctgc cacacaagct tcttggacac tggagtcttg
     1681 cctcatgtca atgcttgcta agaagaagcc aaaaatatca acagcttgca tgacttctcg
     1741 caaatccccc tcaatcaaag cggagttgcc attatcaagt agcgactgct caatagcctt
     1801 taaatcagct ttaaaatcaa caggagagtc ataggctaag atgggttttt ctaaactagc
     1861 tggtgagggc caagcagatg tgtttagtcc cacactacta ttcatacgag ctgctggctg
     1921 attagttagt tgaatttgtg tttgtttaag acgcgattga atataatgaa aagctctgcg
     1981 ataaggttca tttccacggt aaatcgattg gtcctgagac aaactggcta aacgttccac
     2041 ttccgagttg ggttgtacca aggttgacga cagtgaaaat gttcggtaaa gggctgctaa
     2101 tttgtcaatg tagtaattga ggataacctc actttgaacc gtagctgaca agcacaaggt
     2161 ttcagctgtg acaaaggggt tgccatcacg gtccccacca atccacatcc ccatggtaat
     2221 gggtttggga ttgtctaact ctagaccgtg tttttgggct aagtttttgt actctgttgt
     2281 caacttggtc actgcttgaa tgagagaacc atcgtaatac tgcatgacat tttttatctc
     2341 gtttttcacc tgaagctttt tctcgcgaat aatatctgtc tgcatgatca tttcaatata
     2401 acgatatagc tcctgacgcc atttctcaag attaatcaca ccagccttag catcacggta
     2461 tttgcgcaga agtttatgaa tgtgagttgt caattccaga atcgttttac gttgtacttg
     2521 cgtcggatgt gctgttaaaa caggaaccac attaacctgc tccaaaatgt ctttaccatt
     2581 atcttttcca gcaagatcct tgatggttag ggctaatttt cctaaataat cagtatccgt
     2641 attattttga tgattaattt cataggctaa atcgacatct tctgaaatat tgattagcaa
     2701 gggtaaaata gaaaaatacc tggaaatcac ttccatttct tgatttgaaa tattggcgac
     2761 aactttttct aattctatgt aatcttgttt ttcagataac accataatag actcgataac
     2821 tgtaaacgca tcatctccaa tcatccgtcg tgtgatattt tccaacattt ctttcaaaag
     2881 agcaacttct tcagcaatga tagtttgatt gttactactc tctaactttt taagtggcaa
     2941 atgaccatct ccttctttgc tagtttcttc tatcatacca ctttttacca aaaataaaag
     3001 tgagcatgtt cataacgttt gttttttaac aaaagtaagc gctttatttc aaaaaatgtt
     3061 tggataggtt tagtctccat attccactaa aattctgtta tactagtctt gactatattg
     3121 ttaaggagac aactatggaa ttgaaaaaac gttcagaatt ccctgaaaat gaactttggg
     3181 atctaacagc gctctacaaa gaccgccaag attttttact cgctattgaa gaatccttac
     3241 aagatattga cctgtttaag cgcaattatg aagaccgctt aacatcggtg gacgatttca
     3301 ctcaggcctt aatagaaatt gagcatattt atattcaaat gagtcatatt ggcacttatg
     3361 cctttatgcc tcaaaccact gacttttcag atgaaagttt tgctcaaatt gctcaggctg
     3421 gcgatgattt catgactaaa gctagcgttg cccttagctt ctttgacacc gccttagcca
     3481 atgccgacct tgacgtgtta gatacattag aaaagaaccc ttatttcagc gcagctattc
     3541 gcatggcaaa aatccaaaaa gaacacctct taagtcctga tgtggaaaag gctttggcta
     3601 acttacgcga ggttatcaat gcgccctatg atatttacac caaaatgcgg gctggtgact
     3661 ttgacatgga tgattttgag gtggatggta aaacctataa aaatagcttt gtttcttacg
     3721 aaaatttcta tcaaaatcac gaaaatgctg aaattcgtga caaagctttc cgttccttct
     3781 caaaaggtct ccgtaaacac caaaatactg ctgctgctgc ttatttggcc aaggttaaat
     3841 ctgaaaaatt gttagctgat atgaaaggct acacttctgt ctttgattac ctcctagctg
     3901 aacaggaagt ggatcgtagc ttgttcgacc gccaaattga cctcatcatg actgaatttg
     3961 gaccggttgc tcagaaattc ttaaaacatg tcgcccaagt caatggtctt gagaaaatga
     4021 cttttgctga ctggaagcta gatattgaca atgatctcaa tccagaagtc agcattgatg
     4081 gtgcctatga cctcgtgatg aagtctttag ccccattagg acaagaatac acgaaagaaa
     4141 tcgaacgtta tcagacagaa cgctgggttg actttgctgc taatgccaat aaagattctg
     4201 gtggttatgc cgctgatcct tacaaggtcc atccgtatgt cttaatgagc tggactggca
     4261 gaatgtctga tgtctacacg ctcattcatg aaatcggaca ctctggccaa tttattttct
     4321 ctgataatca ccaaagttac ttcaacaccc atatgtcaac ttattacgtc gaagcgccat
     4381 cgacctttaa tgaattgatg cttagtgatt acttggaaca tcagtttgat gaccctcgtc
     4441 aaaaacgttt tgctcttgct caccgtttaa cagacaccta ctttcataac ttcatcactc
     4501 acctattgga agcggctttc caacgtaagg tttacaccct gattgaagaa ggcggtacct
     4561 ttggagctga ccaattaaat gcaatgatga aagaggtctt aacagacttc tggggagatg
     4621 ccgttgacat tgacgatgac gcagctctta cctggatgcg ccaagcgcat tactacatgg
     4681 gcttatatag ttatacctat tctgctgggc ttgttatgtc aacagctggt taccttaacc
     4741 ttaaaaacaa cccagatggg gcaaaagaat ggcttgactt cctaaaatca ggtggcagcc
     4801 gtacgccttt tgatactgca atgctcattg gggcagacat tgccaccgaa aagcctcttc
     4861 gagataccat tcaattcttg agtaataccg ttgaccaaat tatcaattac accgaggaga
     4921 tgtcacatgc ctaatctcta tgattttacg gttaaggctc aaaacgggaa tgacctttcc
     4981 ttagctgctt ataaaaaaaa ggttgttttg attgttaaca cagctaccaa gtgtggatta
     5041 accccacaat accaggccct gcaagcacta tatgacacat accatgacaa gggatttgag
     5101 gtcttagatt ttccttgcaa ccaattttta aatcaagctc caggagatgc tgaggagatt
     5161 aatcactttt gcagtctgac ctatcacact actttccctc gctttgctaa aatcaaagtg
     5221 aatggtaaag acgctgatcc tttgtttgcc tggttaaaag aagaaaaaag tggtcccctt
     5281 gggaaacgca tcgaatggaa tttcactaaa tttctcattg accaaaatgg tcaagtcatt
     5341 aaacgctatt cctcaaagac agatcctaaa ctaattgaag aagaccttaa agcacttctt
     5401 ggttaatata aaatagactt ggcgtgccaa gtctatttta tattatagtt actatttaaa
     5461 aacttcttga taaaattcaa tgctatcacc atctttaacg gtaatttgat cgacaccctt
     5521 atctgccatc tttccattga ccttaaaaag ccagtatttg ttagctttcg tgtcttgctc
     5581 gataccatca attgctgtga taaaaccatc tttttcttta acctcataat tatctttgag
     5641 aatctcaagc accgtatctc cttttccaaa agaaactttt tcatcaatgg tattggtatc
     5701 ttctttgaca actaagcgaa cgtgatggtc tgcttttgga acgcttggag tcgtctgtat
     5761 ttgttttgtt ccttgcgagc aagcaacaag tagcatagca acaagtgcta gaaatcctgt
     5821 tttaaatctt ttgttcaccg attaacctcc taaataacat catcacaact ggaaaaaaga
     5881 cgagagttga taaagcatgc gctatattaa agggcatccc tgctaaaaca tagctccacc
     5941 aaggcatact atataaatac gcaaaacaag tatctatcag caccccataa aggaggccac
     6001 ataaggcgac caagaaagcc tgacagccaa gctgatattt ggggaattgc tgagacaaag
     6061 ggtacaagac aaaacgccat aacaaaagaa caagcacaaa acaagtaact tgccaaaaga
     6121 cccaagggcc aaatcccagc aaaaaggctg ataaaaacaa gcacaacatc ataactaata
     6181 cagcctcggc taacccaaag taaagaagat aactgagtaa aaatgccgtc acaggctgca
     6241 cgttaggcag gctgctaaaa accattctca acacaacaca tagggctgac atgatagcaa
     6301 tgcgactcat aagcttagct gacataaact tcttcctcgt ttttttctct attatgacag
     6361 atttacaaag cgtttaccag tttggagctc acttatttat caaatgataa taacatgaga
     6421 attaaataac aaaacagtac ttgctgactg tcatgatata gtcaaagact aaactaatat
     6481 tgtaaagaat atagctacca actataagag atagtgacat aaacttgaca tagttagaga
     6541 ggatctcatt tgtaacttct caaagagaat accttaaaat gcaagacaat ataagaaaac
     6601 agttaggata ttttgatttt ttttagtaat tagggtacaa taacagttaa tgagaagaaa
     6661 aataaaacct attgttgtgc tagtcttctt tattttactg gcaatggtat tgattatcgg
     6721 aaaaagacaa gctaatcatg cgaaacaaaa agaagttgaa gacgctaaga gtcatattcc
     6781 tattgcaacc agtaatccta gtaaagcaaa agctagcact tctgaaaccg aagactttat
     6841 tttaaatcct attgttgatg tttccggctg gcaactccct gaagaaattg attatgacac
     6901 attgtcacgt cacatctctg gcgcaattgt acgtgtctat ggaggatcgc aaattacagc
     6961 tcataacaac gctgctttta caactggaat tgacaaatct tttacaacgc atatcaaaga
     7021 atttcaaaaa cgaaatgttc ctgtagctgt ttacagttac gcacttggtc gtagtactaa
     7081 agagatgaaa gaagaggccc gagctttcta caaaaacgct gctccctata atccaactta
     7141 ctactggatt gatgtcgaag aagccactat gaaagatatg aataaaggcg taacagcctt
     7201 ccgagaagag cttaaaaaac ttggtgctga aaatgttggt ctctatattg gaacttattt
     7261 tatggcagaa caagatattt caacaaaagg tttcgatgct gtatggattc cgacttatgg
     7321 tagtgattct ggctattatg aggctgctcc taatacaacc ttagattacg atttacacca
     7381 atatacctca caaggttatc tcagtggttt taacaatgct ttagatttaa atcaaattgc
     7441 cgtaacaaaa gacactaaaa aaacgtttga gaagttattt ggcaactcca acaattaaga
     7501 ggttagcatc atggctaatc ttttttgtag tttacatctt accaagaaaa actctaggtc
     7561 aataatttaa ccccccttat ccttgacaag gagattatct catcatataa tgaaagcact
     7621 ataatatttc acaaaggagt gctttggctg agatcgcaat tgcgaaatcc tgaggacctg
     7681 atcttgttag tacaagcgta gggattgtga ccaataatca atgattggaa agcgatactc
     7741 tttttgtgca caaaaggagt tttttatgtc accaaacacc aatgtcaaat accttatcga
     7801 agctgctatt tttgctgcat tggcgatggc cctttcattt attccagatt ttgctggctg
     7861 gtttagtcct tcttatggtg ctattgcact ggttattttc tctttgcgcc gtgggttaaa
     7921 gtacggcatg ttggcgggtc tcatttgggg gctgcttcac tttgtacttg gaaaagttta
     7981 ctatctcagc atgtcccaag tctttattga atatatctta gcctttacct ctatgggatt
     8041 agccggtagt ttctctgata gtttgattaa aactttaaga agacaacaaa ccttttttgc
     8101 cgtttttctt gcaataatgg ctagtttact agccgttaca gtacgttact tgtggcactt
     8161 ccttgcagga atcattttct ggggcagtta cgcccctaaa ggcatgtccg ctgtctggta
     8221 ttcttttagc gttaatggta cagctggtgt cctaactttt ttaataacat gtttagcgtt
     8281 aatgattgct ttaccaattc atcctcaatt atttgatccc aaagactaat ttttagtcat
     8341 ttgaatctta cattaaaaaa atggtataat aatgttgtta taccattttt ttatgtttag
     8401 ataaaggtaa ggacttgata tggctaaaaa aacaaaactc aaaaaaacct tagtagaaca
     8461 aatcctcgat aaggctaata ttgctcatca aggactcaag cttaatgctt tagaaggcga
     8521 ctttccagat gaccttcaac catcagatat ttacaagaca cttgctctga ctggtgatca
     8581 aacaggtcct ctaattggca ttattccatt aacagaacac ttatcagaaa aacaattggc
     8641 aaaagtatca ggtaacaaaa aagtgtccat ggttcctcaa aaagacttgc aaaagacaac
     8701 aggctatatt cacggtgcca ataatcctgt tgggattcgt caaaaacatt catatcctat
     8761 ttttattgac caaactgcac tggaaaaagg tcaaataatc gtttcagctg gtgaagttgg
     8821 gcgttctata aagatttcta gccaggctct agctgatttc gttggcgcaa gctttgctga
     8881 tttaaagaag agaaaatgaa cactactgcc aactttatct ggtttctttt ttaagttttt
     8941 cgccttgatt aagctagtct ttgtttagta ttattttgat ctcaactgct ttaacattga
     9001 tcctgttaat ctttttcttg tgtaaaggct tgatatcacg acaaattcag gtcgatttgt
     9061 cttaaatcta atatgggctt tacactttaa tcagaaccta gctattatac acttatacta
     9121 cgctttttta ctttatcttt tcactatagg tattattttt gcttggtgat gcctaaactt
     9181 tttctggtta cttttttatt tttagcgctc gcatcagtta tttctaaaag gaggcttaaa
     9241 tgaaattata ttttgtaaga catgggaaaa ccctttggaa tttagaaggt cgctttcaag
     9301 gcgctggggg cgattcgcca ttacttgaag aggcaaaaga cgaaattcac ttacttggca
     9361 aagagttagc taaggtggct tttgatgccg tttatactag cgaccttcaa agagctatgg
     9421 caacagcagc tattatctta gatgcttttg accaacagcc aaaactttac cacaccgacc
     9481 agcttcggga gtggagatta ggtaaattgg aaggggctaa aatagcaaca atggcagcta
     9541 tatatcccca acaaatgctg gcttttcggg agaatctagc ccagttcaaa ccagatcagt
     9601 tcgaggctga atcaatttac caaacgaccc aacgtgtctg ccatcttatc caatcgttta
     9661 aagataaaca ttatcaaaac gttcttattg taggacacgg cgctaactta acagcaacta
     9721 ttcgctcatt attaggtttt gaacctgctc ttttactcgc taaaggtggc ttagataata
     9781 ccagtcttac tattcttgaa actaaggatt atttaactta tgactgttta atttggaatg
     9841 ataaatcttt tctagaggtg taaaaccact agacaataat aaaaaaagct aacacattga
     9901 tctcttactg acaagacact cactttgcta gcttttttta ttattatcta gtgggctatt
     9961 atggtttcat ctatctggca tattttaagc gcaaaacttt ttcttgctgc ttggttaatc
    10021 gaagcaagat aaccactttg tcaatgctca tgttgctttc aagtaaacgc tctgctgcca
    10081 tcatcaagcc attattgatt cccatctgta aaattggatt tttcttgtca ttgacatcaa
    10141 aaataaattt attatcaggt aaatcaaaga gtactttaac ggcaccaaag agctgttcaa
    10201 agttatcaat agccgcatca taaacaggct tagttcctgg ttttagctga cgacctctat
    10261 gattaaacca catagaatgc caaccaccat taaaggcacc catgatatca ttgtcataag
    10321 agtccccaac atatagtgtg gtttgtgggt tcatgtcaaa ttgttctgct gccaggttaa
    10381 aaatttcttt ttctggtttt tgaaagcctg ttgcttggct cacaatgaca cgcttaggat
    10441 caatataatc ataaagccct aatttacgaa cttttttcag ctgatgttct gtgggcccat
    10501 tggtaataat ccccatagga acattctttg attttaaaaa gtcaagggtc atacgcatct
    10561 catctaacat ggtaatgttc tcaagttcgt gctcatagac ttcttgaaaa tgtactcccg
    10621 cagcttcgtc gatttctcga tagccaaact caagcagtgt ttccttgcaa cgccaaaatc
    10681 ggaaatattc tgtggtccac tcccctgcca ttactcttgg gaatccaaca tctgaatagt
    10741 gacgaaagcg aatataagct tgattcatta ctgacatatc aaaatctggg aagcactttt
    10801 ccatggcgat tcgataaggc gcttgctggt cgtaaattgt gtcatccaca tcaaaaacaa
    10861 tagcagtaat catgagtctc ttttcttttc tagtttaata tcccaatcat tatactattt
    10921 tccattaggc ttttcaattt tattttcaaa aaagtgcccc gctgcagtcg cattttccaa
    10981 cgaaatatag gtgtattcta gcctctttta tgatacaatg gaaaggaaat cgtattggag
    11041 gaaaatatgt ctaatcaaca catcgaagaa ttaaatgacc aacagattgt ccgtcgtgaa
    11101 aagatgatgg ctctggctga acaaggaatt gatcctttcg gtaaacgctt tgaccgcaca
    11161 gccaattctg ctgaattaaa agaaaagtat gctgataaaa caaaagaaga attacatgag
    11221 ttaaacgaaa cagctattgt tgctggccgc ttgatgacaa aacgtggcaa aggtaaggtt
    11281 ggttttgctc atcttcaaga ccgtgaaggc caaatccaac tttatgttcg caaggactct
    11341 gttggcgaag acaactatga aatcttcaaa aaagccgatt taggcgactt tatcggcgtt
    11401 gaaggcgaag tcatgcgtac agacatgggc gagttgtcaa tcaaggcaac caagttgact
    11461 cacctttcta aatcacttcg tcctcttcct gaaaaattcc acggattaac tgatattgaa
    11521 accatttacc gtaagcgtca ccttgattta atttcaaatc gtgagagctt tgaccgcttc
    11581 gtgacccgtt ccaaaatgat ttcagaaatc cgtcgttatt tagacggtct tgatttccta
    11641 gaagtagaaa cgcctgtgct tcataacgag gctggtggtg ctgctgctcg tcctttcgtt
    11701 actcaccata atgctcaaaa cattgatatg gtgcttcgta tcgcaacaga acttcatttg
    11761 aaacgtttga ttgttggtgg tatggaacgt gtctatgaaa ttggtcgtat cttccgtaac
    11821 gagggaatgg atgctactca taatcctgag ttcacttcta ttgaggttta ccaagcctat
    11881 gcagactatc ttgatatcat gaacctgaca gaaggcatta tccaacatgc tgctaaagct
    11941 gttaaaggtg atgggcctat tgattatcaa ggaactgaaa tccgtatcaa tgaacctttc
    12001 aaacgtgtcc acatggtaga tgctatcaag gaagtgacag gtgttgattt ctggcctgaa
    12061 atgactgtcg aagaagctat tgcacttgcc aaagaaaaac aggtcccact cgaaaaatac
    12121 ttcacaagtg tcggacatat catcaatgct ttcttcgaag aattcgttga agaaaccttg
    12181 gtccaaccaa cctttgtctt tggtcatcct gttgaagtat cacctttggc taagaagaat
    12241 ccagaagata cccgcttcac cgaccgcttc gaacttttca tcatgaccaa agaatacgcc
    12301 aatgctttca cagagttaaa tgatcccatt gaccaattat ctcgttttga agcacaagca
    12361 caagctaaag aacttggtga tgacgaagca actggcattg actacgactt cgttgaagct
    12421 cttgaatatg gtatgccgcc aaccggtgga ctcggaatcg ggattgatcg tctttgcatg
    12481 ttattaacaa acacaacaac aatcagagat gtgcttttat tcccaacaat gaaaccttaa
    12541 cttttttaag atttgtattc atatcttaaa accgataagt ggcaatataa cataacgttt
    12601 tattatctct aaccagtctt cattaccatg aagactggtt tattaacagt agcttataga
    12661 aagtgactta acaagatgac caaagaatat atctcctaca ttagtgctgc tatcttcttg
    12721 ctttatggcc ttttagctgc caaatggatt tttcttgggc taggcttaat tttggtactt
    12781 gtcggccttg ctgaccgcct caattcaaaa aaataatcct tctattcgct cctgtccttt
    12841 taacctagcg atgtcatcac caggttaaaa ggacaggggc gaacgaggga ttatgtttcg
    12901 tttaaggtaa cattacttac gaaaagcgtc taagatggtt ttaaattctt cattagtaag
    12961 agtaatgcct ttccccattt tcgtatggtc tggactccac gtgcggatat cccatttcgc
    13021 ttctgcgcca ttaaagctga cgcggttcaa ttcttttgtc caacctttat cattttctga
    13081 taaggtgagt aaatgttcct caatgttgaa tgtaaattct gccattagtt ttcctcctca
    13141 tctatactat tcgaaattaa agtggaaaaa atcatctttt tttcactttt ttttatgact
    13201 cattctccta ttattctatc aagttttgag aaaataagct attgtgatat ccaactttca
    13261 aaaaaaaaca ggctctcttt tcccaactat ggtcgtatac catttggaaa aagagggcct
    13321 ctagtatttc ttgttattat gttctaacag ttttactggt gccatctttt tgatagaggt
    13381 tgacaagacc ttccttgcaa gccctaatca tatcccctgg cttaacttct ctcggtaaag
    13441 agatggttaa gagttccatt ggatttgggg cacggtcaat cttttggcca tccgcatcat
    13501 gtaagtcctt aacaaccgtt tcaaaatgac ggaaacctgg tccataacat tcaatccgat
    13561 cgccttccat gatgacatta cgctgacgaa tggtcgctgt catgctggct gagtcaaagg
    13621 caactacttc tccgacaaat ttatattgtg gaattttgcg gcgagcacca aataattgtt
    13681 cattttcagt tgggatacca tagtaaaaac ctgtagccaa ctcgcgctgg gcaaccttcc
    13741 acaactcgtc aatcaattcc tctttgatag cataaaaagc ttctgggctt tccatgtaag
    13801 catctacagc cgccttgtaa cagttggtta cggttgagac gtagtggata gatttcattc
    13861 ggccttcaat ttttaagcta tcaaccccat tttcaatcag gtcaggaata tggtcaatca
    13921 tacacatgtc aacagaggac atagaatagt cttctggaat ttcccctttt aaggagcggc
    13981 gctctcctcc aaatggcatg tcatacaaat catacttcca gcggcaagac tgtgagcagc
    14041 cgcccctgtt ggcatcacgg tgactcatgt ggtttgacaa aacacagcgg cctgaataag
    14101 agatacacat ggctccatgg acaaaggctt caatttccac atctgtccgc ttacggattt
    14161 ctgctaactc ggccatatta acctcacgag ctaaaacaac tcgggtcaaa cccatggctt
    14221 tccaaaattc aaaggtctcg taattggtag atgaagcttg cgttgacaaa tgaatttcca
    14281 aacctggggc ttctgttgaa caaataacaa tcaaggctgg atctgaaaca atgaccgcat
    14341 caagccccat atcacgcagt tgacgaaacc actcgcccgc accaatttcg ttcccttcgt
    14401 gggtaaccat gttggcagca acatagacct tagctccacg cgcatgtgca taatcaatgc
    14461 cttcttgcaa ttcttccata gagaagttac cagcgcggct tcttaggcca taggcctgtc
    14521 ctccaacaaa aacagcatct gcgccatagt caatcgcaac ttttaatttt tcaagtgttc
    14581 cagcaggtga taagacctcg ggacgttttt tcatatgtga catgtgatat tctttctatt
    14641 ttcaagatag gtagattacc tgtgtaatgg tgtcataacc ctgtcttgcc ctgaaatagt
    14701 aaactcactc aagacaagga ttacttattt aacggttttt ggatcgaact cataaaagcc
    14761 agtatctaat ccacggccag ctggatgatg ggcatgcact gcttgatcaa gcttttcagc
    14821 ttcttcttgg gtaaattgcc cagcttctaa taaggtttta gcctgaataa acaatttagt
    14881 aatagcgaca aaatcatcac ctggacagta aatgccatct agcttccaat gagttaagcc
    14941 atgcgcatac agctcaccca atttagacat catgtcgata tcgttattaa tgaaaatatg
    15001 agtgccatga ttgtcttcat aaatggagta atggctgcta gcatctcctg gttctgccaa
    15061 gaaaagtcct cgttcacgac taacctcatc atcaatcttt gtaaaatggt aataattttc
    15121 tagcaaggga cgtttcgagt gatgaatcac cgaggcgccg taaactagca cctctgctgg
    15181 aaattccaaa ttttcagcta aggtgaataa ctctgctgat gggatttccc ttgctaaaac
    15241 actttcaaca gcaccatgtt gcccccagaa atttacctga cggctagaag tcacaaaaac
    15301 agaggtatca taaatcagtt taaagttata gccatccctt ttattgacat aaaacacacc
    15361 tgcatcacca accacaagat aatcaacagc aatctctatc attaagtcta aaaagggttt
    15421 gatctgatcc atcatatctt gatgcatcaa agcattgcaa gctactgtca actctttgcc
    15481 tgcatcatgc actagtttgg caatttgtct caattcatca taagaaaaat tatggggaag
    15541 acgtaacccg taattggctt caccaacata aatacgatca acaccagcag ctaataaagc
    15601 tttgacttgc tcaatgcttt cagctgtggc tgtaataatt attttttcca tataagcttt
    15661 attataccaa aaattttaag accttaagca tatttcttgc aaaagcccaa acgttaagct
    15721 agtcccctta ctgttatagc atgataagtc acttgtcatt agtaaatctt ttcaaatctt
    15781 ttaaaaaaag atgacaagaa gagactttta tgatagaatt acttagtatg ttaaggaaag
    15841 gagaggacta tgcctgcacc gttaaagaag cataattcgg ttgaacagga tgattcttac
    15901 caagaatctc aaagtaaatt tcgagatttt caagagttca atggccaaag tgcaaagcta
    15961 aaagaattag tcttctttgc tcgtatcgct agtttcagcc ttattaccgt tttatttgca
    16021 tttgtcctgc tcgtaatgaa tttagcgcca atgtttgctt tcttatttgc aagcatcatc
    16081 agcttaggca ttacttcctt attagccaag gttatcaaat cattactgat gtaacttcca
    16141 gagtccattt tgtatgactc tttttctttt gccaaaaaca aaaaaagatt gaagccacaa
    16201 tccccccctt cttcagttag cctaaaacta accatcggga ctagtgatca gatccttcaa
    16261 tcttttcttc ttctatgttt taggtcgttt tagttagtgt ttgtctaatg tatcactaac
    16321 aggctcatct tgttcagtat agtcaatgat gatgtcgtct tcatttaaat caatgataac
    16381 atcatcttgc ttttctttcg cttcaacttc ggcaagggtt tcttttgttt tttgcacgaa
    16441 agctgttgtt ttatctttga gcaagtctaa catgtcctct ttagttaagt caccagaagc
    16501 caacttatct ttcacatcat aaaaagtgtc acgtgctaaa tggctgtatt cactaccttt
    16561 ttctttagca aattggtggt aatcatctgg actttctttg taggcttgat aagctttttc
    16621 agcacgattt ttcaaagcct tgcctttttc tgttgataag aagtaagctg ccgctactcc
    16681 tgaaacagct ccaatcacta agtttttgaa acttttattc atgcttttct cctttttttc
    16741 caaatacttt tgaggctact ttaccaacta atgctaattt tccagctttt gaaacattac
    16801 ctgtagcatt agttgctttt tgcccaaaat gccttgcttg actatttaaa tctgaaagac
    16861 tttctgacaa atcagcaata gcaacaaaca gtggatcgat ggttgctact ttaccattaa
    16921 catcttcaac aaggatatta gcttttgcta aaatatcatt agtttgatga agtgtcacat
    16981 taacatcact tgtcaataca gaaatcgttt ttttggcttc atcaattgtt tctgagactt
    17041 tctttaatac aataatcaag aaaatcacca aagcaacaaa tgcgagtgca ataatcatca
    17101 gtgagattcc aactaagtcc ataagtttct cctttttcta tccttattct tgataataag
    17161 aaattccttt ttgacggcgt ctttttatga caaagattaa gccaataata attaataagg
    17221 cagacacata ttgggagatg cgaataccta aaaacataag actatctgtt cgcatacctt
    17281 caatgactag tcttccacta ccataccaaa ttaagtaaaa tgcaaagatt tctccatcta
    17341 atagactttt tggcttacga cgccacatca taataatgac aaatcccaaa agattccaaa
    17401 gtgattcata gagaaaggtc ggaatgcggt aacttccctc tataaacatt tgcttttgaa
    17461 taaagcttgg taagtaattt aactggctca cagctttacc ataggcttct tggttgataa
    17521 aatttcccca gcgaccgata gcttgagcga ccatgacact tggtgcggca atatctaaaa
    17581 aatgaatggg attgagcacc ttgttataac agtaagccaa aagtacaaga gctcctgtaa
    17641 tgagaccgcc ataaatagca ataccgccat tccaaatggc tatgatttca tctaaatgct
    17701 tgacataata agaccattca aaaataacat agtaaattct tgcaccaaca atagcaagtg
    17761 gaaaagcaat cagaataaaa tcgaaaatag cgtcagatga tattcctttt ttgggtgctt
    17821 cttttgatgc taagtaaacc gccaaaacaa gtcctgacaa gatacatagc gcataccagt
    17881 ggatggctag tggaccacat tttaaggcaa tgggattgat cattgactca cctcattttt
    17941 ggtgattagt tgagttaaac gatcttcaaa agtttttgta gcatcaaagc ccatttcttt
    18001 agcccgatga ttcatcgcag cggcttcaat aaccactgaa acatttcgac ctgtttttac
    18061 cggaatacga atacgaggga ttctcactcc tgagaaagtt atctcctcat taccgtttcc
    18121 gagacgatca aaaactttac cagcctcaaa gttttcaagg taaatggcca attgaacttg
    18181 tgatgagtcc ttcacagcac tagctccata aagcgacatc acatcaataa tacctacccc
    18241 acgaatttca agcaagtggc gcaagatctc tgctggctct ccccaaaggg tttcttcatc
    18301 tttggcatag acatccacac ggtcatctgc aaccaaacga tgccctcgct taactaattc
    18361 taaaccagtc tcacttttac caattccaga atccccttgg ataagaaccc ccataccata
    18421 aatatccatt aaaacgccgt gaacactagt acgttcagct aaagaagcgt caagaaaata
    18481 agacatttct ccagctaagc ggctagttga aacacggcta cttaaaagtg aaataccttc
    18541 ttcttttgca gcttgaacca tttcttttgg aatggctaaa tttctagaga caaccactgc
    18601 aggagtgtcc tttttgaaca tctcttttaa aacagaataa cggttgtgag aagtcatctg
    18661 tgtaagataa gaccactctt tcattccaaa cagctgcaac ctttctggag cataataatc
    18721 aaaataacca gtcatttcaa gacctggacg tgaaatatct gatgttgtta tctcttttga
    18781 cagcaagtta tctgtagcat aaaccacatc aagtttaact ttttgcacta gcatctttac
    18841 agtaacggtc ataatttctc ctttttcaat attataacac aaatcgaccg ttagaccctt
    18901 gcaaatactc attttttcac tactaaatga gaacaacgac accaaaaatg gtcttagctc
    18961 tattaaaaac taggttttat catttagaga tatgagtcaa aaataagaca atggcaaaca
    19021 aatactttac tagtatcatt gagcaattag tgcttattat ggtatttaaa aaggtatcat
    19081 cttactaagt caaaaagcaa aaagcctcat catagctgat aagacccctc gcttacttac
    19141 atatcaaaac attttattat catcagttaa tcacatggtt gctcttacgt gatgaattat
    19201 cattttaact gattgtttac cagaaccagc catcttcttc ttcatttaag acatcagcgt
    19261 ctttacgacg acgaggacct cggccataac gttcttcaag caagtcttcc ttgtaaggta
    19321 agaagatggc caacaaaata taaagcaaaa ctccaaaacc agttccgtaa ataagaagag
    19381 ccgctaagac acgcgctaga gcaagatccc aaccatactt atctgctaaa ccagcaatga
    19441 cacctgcaac caagcgattt tttcgctgtt tatagaattt agtttccacg tttctttacc
    19501 tcctcattct atgatactag tttacattgt ttttcgcaaa ataaaaccag tcttacgact
    19561 gatttttttc cataagtttc ccgtggcaat ttccacaaac atatttagcc agattaatac
    19621 gcctttttcg ttgataaacc tgcccacagg tttggcaact ataatgatga ttagtctttg
    19681 atttagagct agttgggaca taacgaagcc cgtttacttg agctagcaga tctttgaaat
    19741 ccctatcttt atgatggtac cctcttcctt caaaataaag atggtaatgg cagagctcat
    19801 ggcgaacaat tttccgaaaa ataagctcac cgtgctcctc aagcatccta gggttaaaat
    19861 ccaaatgacc atcttttgga aaaaagcgac cgccagttgt ttttaggcgc ttgttccaat
    19921 aagccttgtg gtgaaatggt ttgccaaaat cagccaggga aacttcttgg acataattag
    19981 tgagtgtcac gcggtggcaa taggctgaga ttgaccttgt ggcggtccaa gtcaatctta
    20041 gagacccaaa cagttaccaa gtcacccact gaaacaactt gactagggtg gttgacaaag
    20101 gttttgctca tttcagaaat gtgaataagc ccatcttcat gaacaccaat gtctacaaaa
    20161 gcaccaaagt caacgacgtt tctcacagtt ccttcaagct tctggccaat ttccaaatct
    20221 ttcaaatcaa ggatatcttg acgtaagatt ggtgcttcaa agtcatcacg aaggtcacga
    20281 cccggtttaa ggagatcagc aatgatatct ttaagggttt cctgcccaat agccaatgtt
    20341 tctgccattt ggggaacttg aactgctgct aaagttgcct tggcagcgtc gtccaagtcc
    20401 tgaataccaa gtaccttaaa gagttctttg actgctgggt aggactcggg gtgaactcct
    20461 gtgttgtcta aaatattttt ggctcctgga attctgagaa aacctgctgc ttgctcaaac
    20521 gccttggctc ctaaacgcgg cacttttttg atgtcagcgc gtgatgtcag tgctccattt
    20581 tcttcgcggt atttgacaat attttctgaa atcgttttgt tcaaacctga cacatgtgct
    20641 aataaagatg gactagccgt gttcacatta acaccgactt ggttaaccac agtatccaca
    20701 acaaaaccaa gattctcact caactttttc tgactcacat cgtgctggta ctgaccgacg
    20761 ccaatagatt taggatcaat tttaaccaat tctgcgagtg ggtcttgcaa gcgacgagca
    20821 atagaaatag cagagcgctt ttccactgtg aggtctggaa actcatggcg ggctaactct
    20881 gacgctgaat aaaccgaagc accgctttcg ttaacgatga cataggacgt atttgggaaa
    20941 tctttcaaaa cgtctgctac aaaggcttca ctttcccgac tggctgttcc gtttccaatg
    21001 gcaataatat caatctggta agtctcaatg agctgagtca gtgtttcttt agctgcttga
    21061 atcttcgttt ggctagctgg tgctacggga taaataacct gagtagtcaa caattttcca
    21121 gtctgatcaa cgatagccaa ttttgcacct gttcgaaagg cagggtcaaa cccaagaacc
    21181 attttccctt ttaaaggaga cactaacaaa agatgacgga gattttcaga aaagaggtgg
    21241 atagctccat cttctgcggc atcactgagt tctgaacgaa cccgtctttc catagctgga
    21301 acaattttct ttttgatggt ctgattgatg acctcttcaa tataagggtt ggtttctttg
    21361 aaccgcacac tgaaaaagcg ttgcattttc tccaagttat gctcaaaaga cacttttaag
    21421 atgcctaact tttcgccacg gttaagggct aaagtacgat atccttgcat gttagacact
    21481 tggtcagaaa aatcgtaata gatttggaaa acctttttct catccaactg ctcatcttta
    21541 agcgttgata ctaagcggct gtactgccaa atctcattgt aggtccaaga gcgtaattta
    21601 gcatcttctg acatggcctc cacaaggatg tccacagctc ctgctagagc ttcttgtggg
    21661 ctggcaaatc cttctgtgac aaagggttct gctgctgttt caaggttttg cgcattttgt
    21721 aaaatcaaac gtgctaatgg gaacaagcct gcttcacgcg caatcgtcgc ttttgtacgg
    21781 cgtttttcct tataaggcag gtacaactct tccaaatcag cgagtttctc ggttgcttca
    21841 atactcgttc gtaactgatc tgtcagtttt ccctgttcct caattttagc gagaatggtt
    21901 gctttgcgct cattcagggt agtgagagac ttatccatat caataatgga cttaatcacc
    21961 acttcatcta gatttcctgt tacttcttta cggtaacgtg caataaaggg aatggtattg
    22021 ccttgtgctg ttagggcaag cacctgttca atttgatgga gactaactga caaagcctct
    22081 gcaatgttgt gattattgtt attttccata atattgctat tataacacaa accgtgttaa
    22141 tgcaaaaaat gatagcgaaa cattgtccta atataaagtt gcgtaaagaa tcaaaaaaag
    22201 ctacttttag taaataactg acgaaaccct aaataacaat acctaatttt aaaaaagaaa
    22261 gaagtaaaca acacttccgt tttggcattg ttctcaaatg atagtataat agacttatct
    22321 tgaatgaaag aaggtcatag atatggcaat tacccataaa aaaaatgatg aacttgaaaa
    22381 aatgctagca ggctttgcat caatccctag ttttgataag cctttggaag tcaatactga
    22441 tggtaaacta gcgaccaaag aaaaaacgac agaacaaaca aaaccaagca aaccatagct
    22501 agtttgtctc attctcttaa catatcgtcc attcgtttaa actgagttga gagattttta
    22561 gcgatcaggc taacaaaacg tatcagtcga ggctgaacat tcgtgtttca gcctcctctt
    22621 ttatcatcta gaaaggatat ggtgaatgtc actcttttcc aaccttcctc ccagtgtctt
    22681 gcaatggttt gcgattttta tgtccattat cattgaagct cttccttttg tgctcctagg
    22741 aactatttta tctggttgta ttgaagtctt tgtaacgcca gaattggttc aaaaatacct
    22801 acctaagcaa aaatgcttgc gcattctgtt tggaaccttt gttggatttg tttttccttc
    22861 ttgtgaatgc gggattattc ccatcatcaa ccgctttttg gaaaaaaagg tgcctagtta
    22921 tacggccgtc ccttttctgg caacagcgcc tattattaat cctattgttt tgttcgctac
    22981 ttattctgct tttgggaatt ctttgcgctt tctgatactt cgcttggtcg gtgctgcact
    23041 agtagccatt acacttgggg taatgttagc ttttattgta gatgataaca ttctcaagga
    23101 taatgctcaa cccgtgcatt ttcatgatta ttctcatgaa agcttgccaa aacgcattta
    23161 tctagcactg gtacatgcta ttgatgaatt cttcgacacc ggtaggtatc tcgtatttgg
    23221 aaccttgatt gcctctgcca tgcaaatata tgtcccaact cgtgtgttga caacgattgg
    23281 gcacaatccc ttgacagcca ttttgatcat gatgctgatg gctttcattc tatcattatg
    23341 tagtgaagcc gatgccttta tcggagcttc cttattatcc acctttggag tggctccagt
    23401 tcttgccttt cttttaattg gtcctatggt ggatattaaa aaccttatga tgatggtaaa
    23461 ggcttttaaa ggacgcttca ttgtccaatt tatcggcgta tccgttctta tgattgccgt
    23521 ttattgttta cttgtggggg tgctctaatg attcgctttt taattttagc tggctatttt
    23581 gaactaacca tgtatctcca gctgtctggc aaactggatc agtacatcaa tgttcggtat
    23641 tcttaccttg cttatatttc catgatttta tctttcattt tagctcttgt tcaactctat
    23701 acctggatga aaaacattaa ggtacatagc catttaaccg gaaaaatcgc aaggctgacc
    23761 agtcctttta tcttggtctt tcctgttttg attggtctcc ttgtgccaac agtaacccta
    23821 gattccacca cggtttctgc taaaggctat actttcccat tggctgcggg agcctctaaa
    23881 acgggagtca gtgatgacgg taccaccatt caatacctca aacctgacac cagcctttac
    23941 tttacaaagt ctgcctacca aaaggagatg cgtcaagaac tccataaata taaaggcaag
    24001 aaaccggtca ctattaccac tgaaaattat atggaagtga tggaattgat ctatctctac
    24061 ccagatgagt ttgttgatcg tgacatccaa tacactggct ttgtttataa cgaaccgggc
    24121 catgataact accaattcct tttccgcttc ggcattattc actgtattgc cgactcagga
    24181 gtgtacggac ttttgaccac aggtaatcaa accagctacc ctaacaacac atggctaaca
    24241 gttaaaggtc gccttcatat ggaatatgat aaaaacttag aacaacatct acctgtcctt
    24301 caacttgctg aggttcatca aaccaaagag cctaataatc cttatgttta ccgcgttttt
    24361 taaagactaa taaaaaagca cttgtttttt atcaaaacaa atgctttttg cgttttatca
    24421 gttagcatag gaacagagtc accatcttaa acaagaccta tttaggaaaa attatcctca
    24481 aggtaacgtc cattggtttg aatgacttgt cggtaccaat caaacgattt tttcttatag
    24541 cgtgtgagtt gaccagtacc atcatcattt cggtcaacat aaataaaacc gtagcgctta
    24601 cttaattggg ctgttgacat agagacacaa tcaatacagc cccaagcagt gtaacccatg
    24661 acctcaacgc catcttcaat cgctttggca acttgtacga gatgctgact catgtagtca
    24721 atgcgataat catcgtgaac cgtcatgctg ccatctgcgg tttgcaccag ctgatccttt
    24781 gcacctaagc cattttcaac aataaacaaa ggaatctggt agcggtcata gtactgattc
    24841 aagaccaatc gcagaccaat tgggtctatc tgccaacccc actccgaaga ttctaaatat
    24901 ggattggaca aaccacctag aatatttccc tgaccagatg tgtaagcttc aggattgtga
    24961 gcttgtgtga cactcatgta atagctaaag gatagaaaat caaccgtatg ttccgctaat
    25021 aattccttgt ctccgtcttc aaaaacaatc tcaatcccat tttctttaaa atacgactta
    25081 atatatgaag gatacttgcc tctcacatga atatctgaaa ataagagatt aagattttca
    25141 aatgcatgcg cagcaagtat atcacgagga tcagaggtca ttgggtaagc aggcatcgcc
    25201 aaaatcatac acccaacttt aacatcagga ttgatttcat gcgctagctt tgtgacacta
    25261 gcagatgcta ccaattcatg atgaatagct tggtataaat cttgcaggct cagcttttct
    25321 ttttctgtca tgatgccacc actggtaaaa ggcatgtgta aaatcgagtt gacttcgttg
    25381 aaggtcaacc agtatttgac tttgccctta tagcgttcca tgactgtctg agcaaagcgc
    25441 tcaaaaaagc caatcagacg tctatctgtc caaccattat aggctttggc caaatgcaga
    25501 ggtgtctcat aatgcgacaa ggtcactaag ggttcgatgc catagttcaa caattcatcg
    25561 aataagtcat cataaaattg caaacctgct tcattgggct ggtcatcgtc accatttgga
    25621 aaaatacgag accaagcaat agacgttcga aagactttaa agcccatctc ggcaaaaaga
    25681 gcaatgtctt ctttgtagcg gtggtaaaaa tcaatcgcct ctaaagttaa attatcgctg
    25741 gtaggactat ctgtccattc tcctagtcca ccatttggta gaacatcttg tacggacaaa
    25801 ccttttgcgt ctgcctcaaa tgctccttcc acttggttgg cagctactgc gccgccccat
    25861 aagaaatctt ttggaaaaat tcccattctc cctctccttt taggttataa aatgactaac
    25921 aaagcgtcct ctttagtcac catttttttg tcatcttgaa taataatctc caactgctca
    25981 gccgaattgg tcacgaccac aggactgatg agtgaatagc cttttgaagt aatgaaatca
    26041 atatccattt ttagaagtgc ctggcctcgt ttcacgacat cgccaacaga cactaactgc
    26101 tcgaaacctt gcccttttaa ctcaaccgta tcaataccga catgaatcaa gacttccaca
    26161 ccacgtgtgt tttttaaggc aacagcatgc ttggttggaa agaaagtgat gacttcacca
    26221 tcaaaggggg catagaccac accttcgctt ggtaatatgg caaatccttc tcctaataac
    26281 ttggaagaaa aggtttcatc aggaacttct gatagaaaaa gcatctcacc tgtcattgga
    26341 gcatacaacg tttgtttggt tgacaagccg ctcttaactt gtgacatatc tgctgcaaca
    26401 ctcacttgct ttggggactc ttcatcaatt cccatgaacc aagtcaaact gaaggctaac
    26461 acaatcgtcg cagctccggc aatcaaagca tttgtaaaat tagctccgcc tgatgggttg
    26521 atgtactgtg gtaaaccaat aatagaaggt acgacaaagg tataagaagc aatattgaca
    26581 aaaccaataa aggctccaac cagaccacct gaaatcatgg ctgcatagag tggcttttta
    26641 aatttcagtg tgaccccata aagtgccggt tccgtgatac ctgccagcaa agctgaaatc
    26701 cccgcggcaa gggcaacttg acgtgtttgt ttctgctttg ttttaaaggc aacagccaaa
    26761 gatgcggctc cttgagccaa attagaagcc aacattgctg gtaaaatcaa gacatctggt
    26821 gttgcgaccg aagcggccaa aaagattggt gcaaaagccc agtgcatccc cgtcatgaca
    26881 acaagcggca taatcgctcc caaaatggca agtgctagcc acggagccac atgataaata
    26941 gcgagcatgg tattagataa cccttgacca ataatcacac caagaggacc tacaaccact
    27001 aaagccaaaa agccagaaat caaaatgatt aaggttggct gtaagaagct tttcatcacg
    27061 cttggcacca agcgattgac ccatttttca atgtattgca tcaagtaaac catcatcaaa
    27121 atgggaataa cagatgagcc atagctagca ggagtgactg gtgctccaaa taaagccaat
    27181 ggtttacctt ctgctaccat tgcaacaaag gcaggatgca ataaaatccc tgcgatcgtc
    27241 gctgctaaaa caggtgtcac cttaaagcgc gccgctgctg aataggcaat catgacaggc
    27301 ataaaataat aagccgcatc gccaaagaag ttaataaagg caacggtttg agagtccgca
    27361 ctcgctaagc caagcatagg aagcaagatc cccaccactt tgagcattcc accaccaaga
    27421 agggctggaa tcagcggtgt aatcacacca gcaatggttt caatcaagcg ctcaaggata
    27481 cttcctttcg cttttgaaga atgatcttca tcttgattgt caaaatgccc taggcttaaa
    27541 aaggcttggt aataattatt gacatcattg cccaagataa tttggtactg gccgtttttg
    27601 cgcatcacgc caatcacatt tgaaatggct ttgacttgct gatctttaac tttctcatca
    27661 tttttcagta ctaagcgtag acgtgttaca cagtgtgtca cacgttgaat atttgttttg
    27721 ccaccaaccg ccgctaaaat agccttggca gtttcttgat acgtcatatt aagtctcctt
    27781 taaatgtttc actaaacgtt taacatgaac cgtcaaatac aaaagttcgc ttgaattaag
    27841 gtgatagttg taccgctttt ttatctcctc actaatatct aagacgcacc gatattcttt
    27901 agggtatttc gttttcacca ctataagcag ttctgcatca tcatcatcgt aagacatctc
    27961 tgataacaca cgttgagcaa ataatttaat atgggtcata aagcggtaat aatcgatgga
    28021 tgtgtcatcc aattctgttt tgaagtctgt tttgattttt tgctcaatat aagaaacaat
    28081 ctccgtcagg cgatgtggct ccttaaaagg agtgtccaaa ctagcattga caaaatgcat
    28141 ggcaataaaa gctgcttcat caatagctag agtgactcct agattacgct caattagctc
    28201 taaggctttt acccctagac tatattcatc aggataatag cgttgaatct cccaccgtag
    28261 tgggttatga atcaacattc cttgctcatg cctttcaata gcagagtgaa tatgatccgt
    28321 taaattaata tagagaattt catctaaggt tttgcctaat ttaattttac ctagattgat
    28381 aattctctct gagcaagcaa caacttcttg tggcaccgta ataaataact ctgtgaagcg
    28441 attcatattg tcactatttt ttaaaacaaa tgatgtttcg atagcattaa gctcaatact
    28501 atctccaact ttttttccaa aggcaatccc ttttcccatc aataaaatat ctaatccttg
    28561 atggttggtc gaaatcgccg cattatggtt taaaacgcgt ttaatcaaca taaaagtcct
    28621 agtcctttag tatcacaata aaaaatgacc tatccaaaag ctcgctaaaa agcacttttc
    28681 gaataggtcc ctgctcacaa aactgtgata acatccttta atattctgat tgttatgtct
    28741 ctagcctgct tacttgaagt aatcacatct gagaacgctt taatgttatc atctctttaa
    28801 gattctgtca agcgttttca aaaaattctc tgaaagagca actattgtta ttcgtatcta
    28861 ttgatttagc aaaaaagagt ttctgacaat tttaagatta tatgatacaa taacaatcga
    28921 ttagataaag gagttatcat gtcacatcat caacaaaccg tttcaaaaca aacaattatg
    28981 gcgattatcg ccatagcact cattggtttt tcaggaattt tgtctgaaac cagtatgaat
    29041 gtcaccttcc cgacactgat gtcagtctat cagttgccct taaatagctt gcaatggatg
    29101 acgaccattt atttactagc agtggcgatt atgatgacca cttcggctac actgaaaaaa
    29161 aatgtgcggg aaagacccct cttttttatg gccacaggtc tttttacatt tggcaccatc
    29221 ttggccgttc tgacccagtc ctttgcgatc atgttgctag cccgcatttt tcaaggcatt
    29281 ggtactggtc tggtaatgcc tcagatgttt aatattattt tagagcgtgt cccaatgcat
    29341 aaggtaggtc tatttatggg atttgctggt cttattatta gcttagcgcc tgcttttggg
    29401 cctacttatg ggggctttat gattagccat tttagttggc aatggatttt tatctgtata
    29461 ctccctgtac cactgattgc aggtattcta gcttattatt acctcgaaga ttctccagta
    29521 agcgaaaaag taccctttga ctggttggca tttattgcac tatcgattag cttaacttct
    29581 gccttattag ctattactag tctagaaaac ggaagtgtta atttgtatta cttagggctt
    29641 tttattctca gctttgtcct cttcctctac aagaatctca cagctaagca accctttctt
    29701 gatattcgca ttcttaaaat tccctctcta acctttggcc tgattccctt ttttgtcttc
    29761 cagctgatta atttaggcat aaattttcta acgccaaact ttattgtcat ggaaaagatt
    29821 gcgaatagtt ctcaagctgg tatggtatta ctacctggta ccttactcgg agctctacta
    29881 gcacctgctt ttggtaaact ttatgatcaa aaaggagcaa gactttcgct ttatttagga
    29941 aatgccttat ttagtttatc attgattatt atgacacttc aaacaagaca ttttatgctt
    30001 ttaccattca ctcttttata tattttattc acgtttgggc gtaacatggg ctttaataat
    30061 agcttagcca cggctattcg agaattgcct gccgagaaaa atgccgatgc cacggccatt
    30121 tttcagatga tgcagcaatt tgctggcgct ctaggaactg ctatggcatc actgatagca
    30181 aatagtcaac cagaattcac aagcggtgtc cagtctgtct accttctctt tactattttt
    30241 gctctacttg attttatctt tttctttgct atgttttacc atttagggaa aaaaggactt
    30301 gcctaagctg acttacttaa aaaccagcct cgttctgttg aacaagactg gtttttttct
    30361 tataaaatac cttctaaaag acctttcata aagtcttctg agtggaattc tccaatatca
    30421 tccacttttt caccaaatcc gatgaacttg actggaatgt ctaattcttg acggatggct
    30481 aggaccaccc caccttttgc agtcccatca attttcgtta aaattaagcc tgttaaaggt
    30541 gttatttttg aaaattcctt ggcttggcta agcgcatttt gacctgttga cgcatctaag
    30601 gccaaaagcg tttcatgagg ggcatctggt agaacgcgct taatgatacg acccattttt
    30661 tccagctctg ccatgagatt ttctttgttc tgcaggcgac cagccgtatc aatcagaaga
    30721 atatctactt cttttgccac agctttttcc atcccgtcaa aaacaacaga cgctggatca
    30781 gccttttcag ggccagtaat gactggcaca tctactcggc gtccccattc gactagctga
    30841 gccacagccc ctgctcggaa ggtatctgct gccaccaaca tgactttttt accttcttgt
    30901 ttgtaacggt aagccagctt cccgattgaa gtcgttttac caaccccgtt aacacctaca
    30961 aaaagcataa ccgtcaagcc atcttgataa ttaatggctt cattataacg cccgtctttt
    31021 tcatagatat ctaccaattt ttccacaatc acacgtttca aggcatctgg ttttttcgca
    31081 ttttccagtt tggcttcata acgcaactcc tctgtcaaag ttgtcgccac atgtacaccg
    31141 acatctgata agatcagcat ttcttccaag tcttcaaaga attcttcatc cactcgcctg
    31201 aaattggcaa agaaactgtt cagacgagct gagaagccag tccgcgtttt tttcaagcta
    31261 cgcttgtatt tttcttgatc tgtctcagga atggtatcgt aggttgcctt gttttgagaa
    31321 gcttctgctg aggtagaggc ttgattttct tgtggaactt cttctggcat cactggaacc
    31381 gttgccgctg ctttttcttg aaggcttttt tcaagagcag ccttacgacg gtagtagtct
    31441 tccataacct gttggctact ggattgctct tttgaagtgt ctactgcttc ttcattcgcc
    31501 gaatcatttg aggcatcagc ttgggcttgt tttgttggaa actgttccac aactggctgg
    31561 tcaacaaggt cttttggttc tgatgccgta gattgactaa gttcacttac cttaagatct
    31621 gttttgtcgg cagcaaactc attggtatct tctgatgaat gattactatt atcacctgtt
    31681 tcttcctcaa cctgtgcatt tactgaagct gcttcgggac cattttccac cgaacctgcg
    31741 ccctctgaag aaacgtcctc ttgttgaacc acctcaatct tgtcttgctc attggctttt
    31801 tcagaaagct cctctttctg agttgtttct gtcaaaagat tttcttctaa cttttcctca
    31861 gctaccttcg gggtttcttt tttcccaaat aaacgatcaa ataatcccat taactctcct
    31921 cttctaaaat aaatgtcttc acagcttctg caaccccaga ctcatcattg atcaaggtgg
    31981 tcacggcatc ggcaacggct ttagctcctg aaacaccatt agccatagct actccaagtc
    32041 ctgcccattc taacatcgca aagtcgttag cttcgtctcc catagccatg acatgtctag
    32101 cgtcaaggcc aaggtgctga caaagcaagt ttaatccaaa agctttatca atgccttttg
    32161 gcatgatttc aaaaataata tctcgtgatt taaaggcttc aaaatcctca aataagcttg
    32221 gggatagctt tataatctgc tgatccaaaa agtctggatc ggtgactgtc acaattttgt
    32281 tataggtaat gtcttttggt agttccgcta cagatgtcac ctcaataaag gtcaacagtg
    32341 ggttagctag atggtactgc gaatgcctac cgtccttact tggaatgcta tacacatccc
    32401 caccactgat aatatctgtt ggtaagccaa ccgcctctaa agcttgctga atctggcaaa
    32461 cctggtcaaa actgagagag ctcttatcca aaacctcccc tgtatttcgc tgaaccaaac
    32521 ccccattaaa ggtaatactg tagtcatcgt ggtcaagcaa gtccaactct tctagcaagt
    32581 taccgatggc ctttagaggg cgacctgtgg taataaccac cttaacccct ttttctctag
    32641 ccgctgctaa ggctttttta ttggcatctg tgacaatttt ttcagtgttg tagagggtac
    32701 cgtctaaatc aagcgccaaa attctaatat tcatgagtta cactagtcct tttaaatacg
    32761 ttagtactgc tccgtcatta cagtggccaa tgacttggtc tgaaatcact ttgatttcag
    32821 gtcttgcatt ttccgtagca atagccctac cagcaaactc caacatctgg taatcattaa
    32881 aattatcccc aaaagcaatc gtttctgctt tcttaatgcc caaggcttga caaagatgct
    32941 ccataccaaa gcccttattc acctctttta agataatatc aatggaatca aagccggtgg
    33001 tcactgcagt ggcataaggg agggcttgat tcaaccaatc gcttccttct aagacggtat
    33061 ggcctgtgaa gttggtagaa actttaaaaa tggcgtcatt ctccatatct tcaaaaccat
    33121 taataacctt aacattagca tagtaatgtt ttgttttttg aatgtactct tctgatgcgc
    33181 cttttaaaat ataggctgcc ttttgcccag aaaaaaccat acctgtttct acatagtgag
    33241 gattggctaa aacctttttt gcaacttcgg tatactgctc tttggtcatc atatctgcaa
    33301 atagaatctc accacggtat tgaaccacgg aaccattttc tgcaataaca gcaatctgat
    33361 ccaaaaaggg ctcaaagagc tgatcaatgg ctaagagcga gcggccactc gaaaccgcaa
    33421 ataaaatgcc tttttcggct aactttggca agagcgcagc tagttgctct tgattataag
    33481 taccatcttc agctaggaag gtaccatcca tatctgttgc tattagttta atcatacaat
    33541 ctaaccttta ccctttttag ggagtgcaaa attcaaatga atcgtgcatc tcaactccca
    33601 atataacttc tttcattata ccacagataa actaaaaaag gacgtgccga attgatacgc
    33661 ccttgccttt tatcaatcaa tccgtacgcc atttttgaag gttaggctga tgtgcttgtc
    33721 tacttttata tccaaccttt caacacttgt ctgccagagt tctccctttg gaccaggagt
    33781 taaagagatg gttgtaaggt cagcttgtgt cgctagactt tcccctctca cctttaggag
    33841 cggtgtgtca atggaaagat tcccattccc atccctatcc caactaaaga ctggagtatt
    33901 ctgtcctgga ataacgacct tatcagcttc aacttctagg ctcttcacat actctgatag
    33961 aatctgttgg gagataagcc ttgtcatcca tgctgaacta gagacggtca actcatcaat
    34021 cttagctttt tgaagtgctg caactcgtgc ttctaagaca tccgttgcca gataatcaag
    34081 agcagctttc ttatcaaaac gctcgccttc tgctttagca gtagcgatac cataccactt
    34141 ttatctcgta taatctgtac ataggcattt ccccatatca aaagatggct catgattgtc
    34201 tctcgaaaga taaaggagga catctctgga ttgggttcat catgaagaag aaagtacagt
    34261 gggtggtcca gctttttctc cttcccattt tctgtccgtt catagatgtg aataggaagt
    34321 gacgccaccg cttcagcaag aatacgcaca caggcataga ctgccgtcgt ctgcatagtc
    34381 ttaaactcat ccacattctc gccactaatc gtgcgaccaa agaggtagga aaagtcctga
    34441 ccttcataac tgttttgggg cttatctcta gccctttttc ttccaattaa atcaagtagt
    34501 cccataagcc ctccttattt tggggtacaa aaaaagcacc tcctttgaag tgctttcggt
    34561 atatttcttt caaagtaatc aacagtcttt cttacaaaac ctagtattct cattctgtca
    34621 attccttaaa tgcgtcaaga tgtcgtgata atactgaagt cgcaatctca tctaaccttg
    34681 cttgttcaac aactgttgtc atctgctcat caccaaagtt ccatcagttg atttataaag
    34741 gttaaactcc tgattctctt gaatgccaaa tgggtcaggt atcggaagat agatgtcatc
    34801 accaatttgt atcgtcttta cgagttccat acatcactct cccttacttt accaattatt
    34861 catatgacgt gatccataga cgaccgcaac aattataact tcatcttcca gaacatgata
    34921 tacaatgcga tattttttga caatcagttg tctgaaggta tagccttttc caatcagatc
    34981 ctcaatgatg gcacaacgtt caggaaaaat ggatagtgat aacattcctt gggataacct
    35041 ctcaagaaga ttatccgctg cctgtggtgc acagagttca tcacgaacat agtgatagat
    35101 gctcagcaaa tctgctttag catcatccga catggtgacc tgatattctt tcattaagca
    35161 ttttccttca attgagatag aaattctctg acatcctgac ggttgccagt tttcgcatct
    35221 tcaaagctgg tgataagctt atcataaaac ttctcctgac tcatgtaatc aacattaact
    35281 cgttgaggcg cttcaggtag agaaacatca aatgggatac ccccagtcaa aataatctga
    35341 ttcaaaaaca tatcaatcgc agttgacata ggaataccta agcgtttcaa aatatcatct
    35401 gcggcacttt ttactgaatc atcaactcgt aaattcaaag ttcccgtttt agccatggtt
    35461 atcctccttt tttgtaacga cattgtatca cgtttttaga gttgactcaa ctaaaaactc
    35521 aatatccctc gctcatcata gacgctagct tcatcacctt ggcggcgaat gcaacggtca
    35581 agccccagga taagacctac aataccgtca atcttctcaa cagatttttc cttatctggc
    35641 ttgatgttgc ctccaggggc ttgtcgcatc accacgtttt gtcccatcca tttaaggact
    35701 ggatgaccac catgttagat tttcccttcc atcatgagtt tatagagttc cttggacggt
    35761 ggactcatat ccttataact ctgctcaaaa ggcaccatgg tcaagccgac accgcaattc
    35821 caaggtatct tctagtaacc atcagagaga agacagggat tgtaaaatca accactgtgc
    35881 aaaatactgc gttcactacc cagtcgaatc agctcaaaaa acaatacggg cagttcttgt
    35941 taacagaatc taataactgt tgtgcatgcc caggctgtga tagaccactc attctaacca
    36001 gtggcaactt agcagcagaa aattacgagg tttcagccat tgaaaaagat aaggacgctg
    36061 aaccactgaa tctgatagcc ctctgcccag attgtttttt aacctatcaa gcagatagtc
    36121 gcaaaaaaat tgtaacagct ctaaaaaatg ttaaaaagat tctagtctct gcacataaca
    36181 gccaacaagc gatttctgat atgaagttag acagtggaat tgtttcagtt ttgactagtc
    36241 tcaataaact aaaatgtgat gaatatgata tctcctatga ctcgaagcaa ttgactgata
    36301 agatatcacc tgagaacaat cgtacgcttt atcaattggt taaaaaccca gttattgata
    36361 actacttaac cattcaaaaa atcatcgtaa atttggacaa acaaggaaga attgattacg
    36421 aggaaatcca atatcaaacg cgatcgatgt ataaaaagtt aaaggctgcc aagcataata
    36481 atctggctat tttcaatact atttcggaaa aactccataa agctacacta caggacatct
    36541 acttctgcca gatgattgtc tcttacttta tccaaaaatg cgaggtactt gaataatgca
    36601 attaccaaat aaactttact cctatcaaaa aagcaccctt gcctatttac ctagagtgct
    36661 aaatgaaatc aaaagtggaa actcaaacgt caaagatatc tttcacgcta tctcagaaga
    36721 gttggatgat cctacagatt tcctatctat aatggattgc ttgtatgcat tgaacgctat
    36781 tgaaatgtct aatgaaggag aagtaaaatt atgcttatag aaatgtggtc tccagtattt
    36841 aagaatcacg gagaaattcg ggaatcaatc tgcttccact atgggttaaa cgtgattatg
    36901 gggatggatt tagctgataa ctctatcggc aaatcctcct cactattagc cattgatttc
    36961 atctttggag gtaatagcta tctaaaatca attgcggtta aaaaattagg tgatcatcct
    37021 atctacttct gctttcaatt tgagaaaaag ttttattttt caagagatac ggccaaccca
    37081 aatatcattc tagtgtgtga taagagctac tctccaaccg gtgaaacaat ggacctagga
    37141 atttttctta acagacttaa gaaacgatat catttagatt ctccagagct atcattccga
    37201 ttagctatga gtggcttctt taggattgca ggaaaaagta atcaaaatac cgattttccc
    37261 ttacaagttt attcgagcca aaaatcttct gaatctatta ctactctcat ccaactgttt
    37321 aatttgtatg ataacattgc acgatacaaa gaacgtctga aagataagag cgaccagtta
    37381 acaacttttc gtaatgctcg aaagtatacc ttcatctcca acctggttgg cggtaaaaag
    37441 caatttgaag caaatgtctc agaaataaaa agattggaat atgatttagc acatctccaa
    37501 gacactcatc aagataaaat tgactctgat gatattgaga aaaatcaaca aaaactccag
    37561 cttagaaata ctaagctgga gttagaaagc agtcttcggg acaagcaaag acgtcttaag
    37621 ctattggaca tcagtatcga atttggctta tatcctactg aatctgactt aacagagctt
    37681 caacaatact tcccagatac taatctcaaa aaattatatg aagttgaagc ttaccacaaa
    37741 aaattggaaa ctattttaga ttccgaattc tcaactgaac gtgaatccct aatagctgag
    37801 atcgacgaat tagagagtca attaacaact ctaaaccaag aacttcaaga actaggaaac
    37861 atcccaaatc tttcaagtga gtacttagaa agctactcta aactaacagc tacaatcaat
    37921 gcactaaaag aacaaaacga ggcctactta aaggagtctg gtctatccaa agaaaaatca
    37981 gaagcggacg ctgacttgaa acgcagtaca gaagatatcc ttctagatct agaagtaaaa
    38041 atcaacagta agatgcgtga attcaataac attctatatc cagatattcg caaagctcct
    38101 caaattaacc tcaatgctca caatagctac tccttctata caccagatga tgatggtaca
    38161 ggaactaagt ttaaagggat gattgtattc gacttagtaa tgctctacct tactaatctg
    38221 ccagcactca cacacgattc attactcttg aataatgtta gctaccaagc gactgaagca
    38281 cttttgaagc tgtatgacca gtcaaggtca ttgaacaaac aagtcttttt agccttcgat
    38341 aaagccagct cttattctcc agatgctaat caactcttgt cagaaaatac ggtattgaga
    38401 ctctctagta acggaaatga actctacggt atttcatgga acaaaggaga aaactcagat
    38461 gagatttagt tacaataaat tatggaaact tcttattgat aaaggttgga ccaaaacaaa
    38521 gctcaggcaa gaagctggga ttagttcttc cacgatcgca aaattgggaa aaggggaaaa
    38581 tatcactact gatattttat tggaaatttg tattgcactt gattgtaaga ttgaagatat
    38641 agtagaaatt gtagataata atatctagaa ataaaaaaag cactaagttg ataagatgtg
    38701 taagtcagat caacttagtg cctttacttt gaaaaaaata attttctcaa taatacttag
    38761 tactaactgt taacgaatat tatacttttt aaataatatt ctaaatacta atgacaatat
    38821 agtaatgcac gaaccaataa taaaaagatt aaagctattg ttaggattta atgccaccga
    38881 gaaaaagcca gtcccaatag gcataaaaag aatagttatt gtaaagataa taccaaacac
    38941 tctccctaaa aaatcgttat caacatcttt ctggacaaga gaaaaaaatt gaatattaaa
    39001 gatggaaaga aataaactga ataaagctgg tgacaaagct aaaataattg cattatgaaa
    39061 cataatataa aatgggggag ctaacattaa catcagtcct gataaagata gaaacaatat
    39121 caatctcata cttgataatt ctttattcac aaaaccactt aatattgccc caataaatcc
    39181 accaattgct tctgctgtta aaaaagtgcc ataaagtcca gttgaaattt ctccaaacat
    39241 ttgattacta tagggcaaca ataaattata agcggctaga aaaaaattaa caagtgcaga
    39301 aagaacggta ataataaaga tggacttatg gctgtaaaca tatttaaatc ctatttttaa
    39361 atcgttaaat atttctctga ttgtcacctt ctcctttatc accacttcgt cattaacagg
    39421 taaaataaag gaaattagta aagcagctat tagaaatgat agtccatcca atagtaatac
    39481 accgtgtata ccaagtagct tatataagaa aattgctacc ataggaactg ttactttgat
    39541 tacagtactt gttgtctcta gcaatgaatt aagttgtgat atactatctt ttttaacaat
    39601 ttcttttgta aatgctttat aggatggacc agaaaaagca ctcataaatg ccaagataac
    39661 attagttagt acaatggcat aaaccaacca ttgctctttt gttaggaacg agagtactaa
    39721 gcaagctgtg ccacataaga tatttgtagt aataataatt tttttccgct tgaaactgtc
    39781 tgcaatgact ccaccaaata aattaaatag aacacctata acactttcta gagactgata
    39841 tactgcaact aatgatagag acgctggatt aagcccagca agaaaagtat tgttagcaaa
    39901 atcaaacata acatcgcca
//
LOCUS       DATURV010000009        37717 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DATURV010000009 DATURV010000000
VERSION     DATURV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37717
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.009"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(324..1235)
                     /gene="whiA"
                     /locus_tag="VCF75_000465"
     CDS             complement(324..1235)
                     /gene="whiA"
                     /locus_tag="VCF75_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEP2546058.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSFIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFDLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYLQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            complement(1232..2209)
                     /locus_tag="VCF75_000466"
     CDS             complement(1232..2209)
                     /locus_tag="VCF75_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEP2546059.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNENDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            complement(2206..3096)
                     /gene="rapZ"
                     /locus_tag="VCF75_000467"
     CDS             complement(2206..3096)
                     /gene="rapZ"
                     /locus_tag="VCF75_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEP2546060.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYQVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            complement(3511..4857)
                     /gene="asnS"
                     /locus_tag="VCF75_000468"
     CDS             complement(3511..4857)
                     /gene="asnS"
                     /locus_tag="VCF75_000468"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEP2546061.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVTNKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVTKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            complement(4878..6071)
                     /locus_tag="VCF75_000469"
     CDS             complement(4878..6071)
                     /locus_tag="VCF75_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP2546062.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            complement(6406..8907)
                     /locus_tag="VCF75_000470"
     CDS             complement(6406..8907)
                     /locus_tag="VCF75_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEP2546063.1"
                     /translation="MFCFIDIACYNRLTMIQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLSHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFTR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRKDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            complement(9015..9779)
                     /locus_tag="VCF75_000471"
     CDS             complement(9015..9779)
                     /locus_tag="VCF75_000471"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEP2546064.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHVDGFKIGILDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            9947..10645
                     /locus_tag="VCF75_000472"
     CDS             9947..10645
                     /locus_tag="VCF75_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP2546065.1"
                     /translation="MPFIFRYSFFNKVLIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            complement(10955..11884)
                     /locus_tag="VCF75_000473"
     CDS             complement(10955..11884)
                     /locus_tag="VCF75_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546066.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(11877..12569)
                     /gene="ftsE"
                     /locus_tag="VCF75_000474"
     CDS             complement(11877..12569)
                     /gene="ftsE"
                     /locus_tag="VCF75_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEP2546067.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            complement(12588..13686)
                     /gene="prfB"
                     /locus_tag="VCF75_000475"
     CDS             complement(join(12588..13613,13615..13686))
                     /gene="prfB"
                     /locus_tag="VCF75_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEP2546068.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            complement(13755..14879)
                     /gene="queG"
                     /locus_tag="VCF75_000476"
     CDS             complement(13755..14879)
                     /gene="queG"
                     /locus_tag="VCF75_000476"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEP2546069.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERHLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILAFMSHLTLKNEDSRKELELIRHKWQV"
     gene            complement(15575..16224)
                     /locus_tag="VCF75_000477"
                     /pseudo
     CDS             complement(15575..16224)
                     /locus_tag="VCF75_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            complement(16521..17156)
                     /locus_tag="VCF75_000478"
     CDS             complement(16521..17156)
                     /locus_tag="VCF75_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEP2546070.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMV
                     TGGISLDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            complement(17161..18162)
                     /locus_tag="VCF75_000479"
     CDS             complement(17161..18162)
                     /locus_tag="VCF75_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEP2546071.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEVNIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMTLTAWQGNRVGLYYLENGFGCRSSQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEVLVREAKKRDI
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADATMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLANQRDVQR"
     gene            complement(18191..18832)
                     /locus_tag="VCF75_000480"
     CDS             complement(18191..18832)
                     /locus_tag="VCF75_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEP2546072.1"
                     /translation="MKIALINENSQAAKNGIIYDALTTVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKGRAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVTYLKSVLEQ"
     gene            complement(18857..19651)
                     /locus_tag="VCF75_000481"
     CDS             complement(18857..19651)
                     /locus_tag="VCF75_000481"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEP2546073.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGLAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            19975..20412
                     /locus_tag="VCF75_000482"
     CDS             19975..20412
                     /locus_tag="VCF75_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2546074.1"
                     /translation="MIAIIVMGHGHFASGIVSALELIAGKQEKVTAIDFTAEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVLGLITCSKEGIVDWKTLSQQEDGTTDDELGGI"
     gene            20412..21611
                     /locus_tag="VCF75_000483"
     CDS             20412..21611
                     /locus_tag="VCF75_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEP2546075.1"
                     /translation="MARSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTSEAREAALKAADKLVQRYQDKGGFIQ
                     AWGVLGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDHDKKTYE
                     AAMHSMLRALIKDYANKDLNPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            21647..22135
                     /locus_tag="VCF75_000484"
     CDS             21647..22135
                     /locus_tag="VCF75_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP2546076.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKYLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKHLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAICCLAHDHQVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            22154..22936
                     /locus_tag="VCF75_000485"
     CDS             22154..22936
                     /locus_tag="VCF75_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP2546077.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGIILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVAIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWSFAILGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            22923..23744
                     /locus_tag="VCF75_000486"
     CDS             22923..23744
                     /locus_tag="VCF75_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP2546078.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVLLTVLIGIIGSWLGILA"
     gene            23831..25738
                     /locus_tag="VCF75_000487"
     CDS             23831..25738
                     /locus_tag="VCF75_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEP2546079.1"
                     /translation="MSLAFTRFKETVNPDFCRNYLLDYQADSYADRKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQKAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LKAKSFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFNALTKQEESLIL
                     ASLEKQLQFLHTNYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPALDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSICLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQINSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGMFLIIDTIQAQGNHCLVSQFILDNHLEVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKLIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALAWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            25802..26797
                     /locus_tag="VCF75_000488"
     CDS             25802..26797
                     /locus_tag="VCF75_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2546080.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGKFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(26881..29562)
                     /locus_tag="VCF75_000489"
     CDS             complement(26881..29562)
                     /locus_tag="VCF75_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEP2546081.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTMLVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(29792..30178)
                     /locus_tag="VCF75_000490"
     CDS             complement(29792..30178)
                     /locus_tag="VCF75_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEP2546082.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            30260..31561
                     /locus_tag="VCF75_000491"
     CDS             30260..31561
                     /locus_tag="VCF75_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP2546083.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEALFHTDHEAFTQEIITNPETEINAILVRHAPDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSRGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMMQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            32017..32826
                     /gene="yidA"
                     /locus_tag="VCF75_000492"
     CDS             32017..32826
                     /gene="yidA"
                     /locus_tag="VCF75_000492"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEP2546084.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            32826..34052
                     /locus_tag="VCF75_000493"
     CDS             32826..34052
                     /locus_tag="VCF75_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEP2546085.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLGIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            34039..35274
                     /locus_tag="VCF75_000494"
     CDS             34039..35274
                     /locus_tag="VCF75_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP2546086.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(35373..36131)
                     /locus_tag="VCF75_000495"
     CDS             complement(35373..36131)
                     /locus_tag="VCF75_000495"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEP2546087.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(36372..37568)
                     /gene="tuf"
                     /locus_tag="VCF75_000496"
     CDS             complement(36372..37568)
                     /gene="tuf"
                     /locus_tag="VCF75_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEP2546088.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
BASE COUNT        11569 a         8035 c         6717 g        11396 t
ORIGIN      
        1 aagaaaaacg aagactctct tggaatgctg atggataacc ttgacattag aaaatcttcg
       61 tatgatgttc aatctaggat agttaagcaa ttgatagata gagtcgaggt tactatggat
      121 aacatcgata ttatttttaa gttttaaaag cattatttag gcactacaac tagtatagtt
      181 gtacatgcca ttatcggtct cctttacaaa cgttattatc ccttttatca tatcacagaa
      241 catcagacac ttcaaaaaaa caatggaaaa cgacttttag acagaaaaat cgtatgaaca
      301 aaggctcata cgataaaaat gttctataag tcatctgcaa ttttattgat tttacgcaag
      361 cgatggttga ccccgctctt ggtgattggg aactcaaggg catcagcaac ttgttgaatg
      421 gaatagtctg gatgcttaac ccgaagctgg gctacctgtt gcaattcaat cggtaaactt
      481 tctaaaccaa ttgtgtccat gattttaata atattattaa tggttttcat gctagcactg
      541 atagttttgg caatattagc tgtttctgca ttattggcac ggttaatatc atttcgagct
      601 tctcgaagta atttgatggc ttcaaagtct tctttgcagg acatggcacc aattataatt
      661 aaaaagtcca tgatatcttc tgccttttgc aaataagtaa ctgcaccgct tttatgctca
      721 atggttttgg catctagcat aaatttttgc atgagttgag ctaaatcttg agcatgatca
      781 agataaactg aatagatttc taactgatac ttgcctgact caggatctct gatgctacct
      841 gccgctagaa aagccccttt aagataagaa cggccagcat tatcgtctga taaaacctga
      901 ggttcgatgc ctgtttccag atcaaaaaag gagtccgcta atttcaaatc ggctaaaatg
      961 gtttccacac cttgctcaac atagacggtg taaacacggt ttttgcgcag gttcgtcttt
     1021 tgatgatacc tgatttctgg cacgataaca taggcatctt caataaaaga ataaatgtag
     1081 cgggcaattt tagcattttc agtggtaatg gataaatgaa ggctttggtg agctaagccc
     1141 aagctgcctg ataatttgat gatggcggcc aattcattgt tgtcacctgt ggacaagtga
     1201 attaattcct ctttgacctt ggttgtaaaa ctcattgctt gactatcctc actaaattca
     1261 ttaattcttc caccactaag ttgccatcgt gaaaagctcc gccattctcc aaccgtaaaa
     1321 aataagacga aatcacacgc ttagcagccc tgcaaaggcc agcaaagtcg tgatcaacct
     1381 gaacgaggta ttcatcaaat ttgttagaat tcatgtaagc ctgcggcacc ttagccacat
     1441 ttaccaaaac cgtatcaatc aaatctcttc ctaagtgctg atttaaaaca gccacatggt
     1501 ctgcatctga aaattgttct gtttcaccat actgggtcat gatattacaa atatacacca
     1561 cttctgcttt agtctggcgc aaagcttctt taatttctgg aatgaccaaa ttaggtaaaa
     1621 tagaggtaaa cagtgagcct ggtcccaaga caatcatgtc actttctaga atagcctcaa
     1681 ccaccttacg actggcctga ggcttttggt cgttgtaggt gttggtcaca tagacatggt
     1741 caatcatgcc ttggtatttg gcaatagaac tttctcctgc tacttcatgc ccatctttaa
     1801 aaacagcatg aagggtcaag gcctgctcgc tagaggggta aattttacct gtaatatgga
     1861 aaaatttggt taaaatttga atggcattgt aggttgagcc ttgcatttca gagataccag
     1921 caataatgag attgcctaga gggtgaccag ccaaggctcc atcattctcg ttaaaacggt
     1981 attggaaaac ccgctcataa aattttggca tgtcactcat cgctaacaag acattccgta
     2041 agtctcctgg cggggctaat tgcatggcat tacgaagttc tcctgaggaa ccaccatcat
     2101 ctgcaacagt gaccacagca gtaatatcaa ctgcttcatt acgaaggctt tttaggataa
     2161 tggagatgcc agtaccccca cctataacag tcattttagg atttttcatg aacgattcac
     2221 cgtttcctta cgacgatttt gatcacgatg gctttcatta accgaccaat ctgttgccag
     2281 actttctgcc aagcaatggg caaaggcaac gctgcggtgt tggcctcctg tgcagccgat
     2341 agccaccgtc aagacagact tcccttcttt ttggtaagcc ggtaagatag ggacaataag
     2401 gtttaacaaa tgcttgtaaa acacctctga ttctgggtga gacatcacat aattaaaaac
     2461 gtcctcatct agtcctgttt tttcacgaag ctctacctga taataaggat tgggtagaaa
     2521 acgcacatca aaaaccaaat ccgcatccaa aggaagacca tatttgaacc caaagctcat
     2581 cacttcaata cggaaagagg cttgattaga cccttcagaa aactggtctg aaatggtttt
     2641 acgcaattgt ctaggggtca attttgttgt atccaccaca tgttggctca tgcttttcaa
     2701 aggagatagg agttctcttt ccaatcgaat accatcaagc acacgaccgt ccgcagccaa
     2761 agggtggctc cgtctggttt ctttatagcg tgacaccaat tctccatccg ttgcatccaa
     2821 aaaaagaatc cgaaaatcaa tgctaggatt gctttcaata ctatctaagg tagaattaat
     2881 ttccttgaaa aacaaacgac ttctcatatc gacaaccaaa gccaccctac gattttcatt
     2941 ggtttgttca attaattcta aaaattttgg aaccaaggct gggggcatat tatcaatggt
     3001 aaagtagcct agatcctcaa aagactgaat ggcaactgtt tttccagcgc cgctcattcc
     3061 tgtcacaata actaaattaa tgtgtttgtc tgacatttac aatttccctt tctctatcga
     3121 accgtccacc ttcattatac cacatacata agaaagctaa gaagtgaaaa agtggtaaca
     3181 cattttgaac tgtaaaaatc agctaatttt aataataact cccgtagctc tcctattata
     3241 tgaatagacg agaccttttt agtatcaaat gagagtgtct atttgataat cgtagtccaa
     3301 atacttgtct ataaaatatc ccttgagcaa ggctatattg tcttatctgc taactgatct
     3361 actaaacttc caaaatttat cttcacaaag gatgtaggtt caaactctac tatttcttat
     3421 ctctccttta ggacatattg atgtttttat aacacactca cttcttacca atacaaaaag
     3481 ttcatagatg acatgaactt ttttgtatta ttagggtcta atccgatgta acatacgtgg
     3541 gaatggaata gcttcgcgaa tatgtttggt accagcaaca aaagttacca tacgttcaat
     3601 acctatacca aaaccgccat gaggaacaga gccatattta cgaaggtcaa ggtaaaagtc
     3661 atattctgat ttatccatac ctagttcatc cattttagtc acaagagcat cgtagttgtc
     3721 ttcacgcatt gagccgccaa tgatctctcc ataaccttct ggagctaaaa gatcagcaca
     3781 aagaacgcgt tctggatttc caggaactgg tttcatataa aatgctttaa agcttgctgg
     3841 gtaattcact acaaatgtcg gtactccaaa gtaattagaa atccaagttt catgtgggga
     3901 accaaagtca tcaccatgct ctaaatgttc ataatctgta tcttcatctg cttcatgctc
     3961 ttgaagaagt gtaatagcat catcataaga aacacgcttg aatggctctg tgatataacg
     4021 tttgagagcc tcaacatcac gttctaaaat atcaagtgct tgaggcgcac gatctagaac
     4081 gccttggatc aatgctttaa cataagcttc ttgaaggtca agtgattcct catgtgataa
     4141 gaaagaatac tcagcatcca tcatccaaaa ttctgttaaa tggcgacgtg ttttagattt
     4201 ttcagcacga aagactggac caaagtcgaa aactcggcct agagccattg ctccagcttc
     4261 aaggtataat tgaccagatt ggctaagaaa tgctggtttc ccaaaatagt ctgtttcgaa
     4321 taactctgtt gaatcttctg ctgcattttc tgataaaatt gggctatcaa atttgataaa
     4381 accattttgg tcaaagaatt cataagttgc ataaataata gcgttacgaa tttgcataac
     4441 agccatttgt ttacgagaac gcaaccaaag gtgacggttg tccatcaaga agtctgtccc
     4501 atgctcttta ggagttatag gatattcatg tgactctcca atgatttcaa ggtcagtaat
     4561 atcaagctca tagccaaatt tagaacgctc atcctcttta acaatccctg ttacataaac
     4621 agatgtttct tgattgagac gtttaataac gtcaaatttt tcaagacctg attcttcacc
     4681 atatttttca atgaagtttg gtttaaaagc gacaccttgg aaaaaagcag aaccatcgcg
     4741 caactgtaca aacgcaatct tacctttacc tgatttgtta gtaacccaag caccaatagt
     4801 aacttcttga ccaacataat ctttaacatc aacaattgaa ataagttttt ttgacataag
     4861 tttctctcta tttttattta attaatttca ttactttcca taaagacatg cagtctacga
     4921 acagcttctt taagggtttc gatatctgta gcataactta gacgaacatt ttccggtgcg
     4981 ccaaagcctg ctccactcac aacagctaat ccgacttcct ctaaaatagc attggcaaag
     5041 cttgtcacat cggaaaaacc tgtcatttcc atggctttct taacgtttgg aaagaagtaa
     5101 aaagctcctt gtggtttaac aacctcaaac ccagggacct gacacaacaa tggataagtg
     5161 atattaaggc gctcttcaaa tgctaatcgc atctcttcaa gtgatgactg actaccacag
     5221 aaagcctcga tagcagcata ttgtgaaacg gttgtcaggt tagaggtagt ctgcccgatg
     5281 atcttactca ttgccgaaat gatctctggt tcacctgctg caaaaccaac ccgccagcct
     5341 gtcatggcat aacttttagc cacgccattg accgtaatgg tttgacgacg aatcgcttct
     5401 gagagtgttg aaataggaac aaactgattg ccgttataga ctaaggaccc gtaaatatca
     5461 tctgccagaa taagaatatc gttgtgcacc gcccattctc caatggccct taattcctca
     5521 gcaccataaa tcatgcctgt tggattggaa ggagaattga taaggacaac tttagtctta
     5581 cttgtccttg ctctttccag ctggtcaaca gttaccttga attgattttc ttctagtcct
     5641 tgaacgaaga tcggctgacc ttctgccatt tttacttggt cagaataaga tacccaatat
     5701 ggtgttggaa tgagaacttg gtcaccagga tttaaaacag ccataaaaaa ggcataaagg
     5761 atgaacttgg cacctgttcc tgccacaatc tgatcaggag acaagtgata cccatactga
     5821 tttttcagat atgtggcaat cgcagctttt aattctggaa gcccagaagc atttgtataa
     5881 aagctagccg taccattttg aatagattca atcgccttgt cttgaatgtg cttaggtgta
     5941 aaaaaatctg gttctcctaa agttaaattc agtacatcac ggccctgcgc tttcaaagcc
     6001 ttagctctag cacctgcagc cagagtaaca ctctctttca tttctaaaac tcgctttgat
     6061 agtttgggca tcgtatctcc tttttatcga cttcttatct tcagattaat taatgatcaa
     6121 cagatcattt tttcataatc tacggtttca gttttaccaa tactcgctac taatcgcacg
     6181 aactatgaca agctgttttg atagcaactg acactaatga aaagaacagt aattgggttt
     6241 actaaaacca aaaatttgtt catttattgt ccttatcaaa aaaacaattc gaaaacattt
     6301 gaacagttgt ctaaaagagc ataataaaag ttcaaactaa tttaaatgtt agttgattgt
     6361 gcttaacgcc attaaaaatt tttctgatat aatagatcct ttctattata tcaaaaaaca
     6421 gtccgtttct actagacaat cgtgaaaatt ttgttgagaa atcaaaaatt cctgaccgag
     6481 accttccaaa ataacttgtc cataggactt tgtaaggaga cgacgatcca aaataataac
     6541 cactgacttt tgatcttttc gacgcatcgt gcgaccaata gcctgtttta atcgcaaaat
     6601 agtcattggc aagaagtaat ctctgaaagg atttttacct ttttccaata gataatgact
     6661 cattttctta acaaagaaat cctctgggtt atcaaagggc aatcgtgcga taagggtaat
     6721 catacgatcc gcttgaataa aatcaactcc ttcccaaaaa gaacctaaac caagcaaaat
     6781 agtttgctct ccttgatcaa accgtttttt aatattataa gctgttccat tcttttcctg
     6841 cgctaagtga ggcacctgcc attggtctag atagtctgaa accaacagca agtgtttttt
     6901 agaattaaaa agcactaaaa taggataacc ctcttgcttc aagctttcga ttcgttttgc
     6961 aattgcttcc acatagattt ggtcactgac ttctgtcacg ataggcgcct cttgatcaac
     7021 aagcaccaac tggtcttgct ttttatcctt ttcaatgaca tgatagagat actcttcaaa
     7081 acctaatagg tctgccaagg ttacttctga gctgatggtt aaagtggcag aaacaaaata
     7141 agttttcacc gtttctggta agagctgttg gaaatgaata aatgccttac tagcactgtt
     7201 tagataagtc actcgcttct cctcttgctt ttcgctacta agccagtagt caccatctga
     7261 ggtagcaaaa aaggcttgta actcttgata atctgctcct gttaattctt tggcgtagcc
     7321 agctatacga gaccaatcat gagcaagctg gtgctcttta ttttgatagt aatcacttga
     7381 cacttgtccc agttcaaacg acaagctttc caataagcgt ttttctaaaa gaggtaaagg
     7441 gttgcttaac ttggcctgta ttgtttgtaa aaaaaccgtc aggttaagct gatgacgtga
     7501 taattgatct aattggagca tcagcttttg ggcctcatca aagaccaaga ccttgtttct
     7561 agtaaagtcc ttgtcatctt gaacacggtg taaaaaataa gcatggttgg taatcaggag
     7621 acgggctgtt ttggcttttt cataactaac tctccaaaaa tcataatcgt aaaattcaga
     7681 agactgctta atgtctccat cgtgcttaag ctgctcaaaa taagcggcaa agcgctgctt
     7741 ttgtttgatt tcgtcaaggt ctcctgtctt agtttctaag agccaaacca ataattgcat
     7801 tttataacga ttcaccaaac ggttctggtc attttggtcc aaactatctg caaagctatc
     7861 tagctttagg taattggcag gcccttttaa actatggcag gcgatgtgaa attgttcttg
     7921 aatagcagct acttcaccag ccattaattg gtcttgaaga agtttggttg gcacactaac
     7981 aatgatttgg ttttgatctt cttttgccaa aaggggtagc aggtagccgt aggtttttcc
     8041 aatacctgtc tgagcttcaa taaaactagc tactccctga tggtaatctt cgtcaattaa
     8101 ctgagcaaat tgagtctgtt taggcctttc ttccagacct aatagtgcca tattaatagg
     8161 gaagcttttt gatatttgat aaggcttgag agcttttgag ccctttggta agagaatctg
     8221 tctgatttta atgtatttgt tgggatcata aggtttggct ttggctaatc cttcttgaat
     8281 gaccattgct gtttcaaaca gcaggctatc tgaatagact aataagcttt ctaagcactc
     8341 aattggaagg ctctcaattt tttgtaaaag tcttaaaaac agtatagctg tagctcttgc
     8401 atctgcaata gcagtatgag cttctgccaa atcaatattg agctgcctag ataggtgact
     8461 aaggttatac ttttcaaagc gagggaaaaa tagttgggct aattcaacag tatcaacacg
     8521 tggtattgtt aactcatatc cttccaaaaa aagagcttct gccaataaat ttgcatcgaa
     8581 ctttacattg tgggcaacaa aaatacaatc ttcaatcaat tgataaatgt gatgcgccac
     8641 ttgaccaaaa tccggagctt tagccaactg cttatccgta atacctgtca aatgaacaat
     8701 atgctcatct aaagattcat gaggattaac atctgtttca taactatcaa taattttatt
     8761 tccttggata ataacaatcc caacctgaat aatagaagca ttgggacctg ctccagtagc
     8821 ttccaagtcc actactgcat attttctaag ttttttctgg atcatagtaa gtctattata
     8881 gcacgctata tctataaaac aaaacatgac tatatctcaa aggtgccatc actccaataa
     8941 ccaagatagg ataatttttt aagaagtata aaaaacactg tgagtttcac tcacagtgtc
     9001 ggcattaatc atttttaatg gaataccata ccaccatcga caactattgt ttgtccagtg
     9061 atataatttg aatcatctcc cgctaggaag cctactgcat tagcaacatc ttctggttca
     9121 gacaagcgtt tcaaagtgat atcttttgca aattgctcca taccccattc atcatctttg
     9181 cctgcatttt taccaacttc atgggcaata tcaaacatca taggtgtttt aacaatacct
     9241 ggtgcgtaag cattgactgt gattccttct gatgccaaat cctttgccaa agtctgtgta
     9301 ataccacgaa ctgcaaattt tgtaccacca taaacagtca aattaggatt cccttcacat
     9361 cctgcttgtg atgttgcatt tataattttt ccgccatgac ctaattctct aaaatgtttt
     9421 tgagctgctt ggcttcccca aatcgttcct ccgacattga ttgcaaaagc cttttcaaac
     9481 tgctcttcag taatcgtgtc aagaggggtt gttggggcaa cacctgcatt gttaacaaca
     9541 acattcaaat caccaaaagt atctacaact ttttggaaag catcaaaaac ttggtcacgt
     9601 tttgaaacat ctgctacaac tgcaacagca tcctcaggtg atagtttatc aaccgcagcc
     9661 tgagcagtct cttcattata gtctaaaatg cctattttaa agccgtctac atgaagacgt
     9721 ttcgcaatag caaatccaat tccttgacca gcaccagtaa caatagcaac ttttgacata
     9781 tcttccacct catatttttt agtcaaccga ttagattgct ctcaaaaagt cagtgttctt
     9841 aaactaagtt cagctttagg cttggcttat tgattacttt tatgatacac ctttatgaaa
     9901 acggtgtcaa gaaattgctg tgagatctca tattttctga aactaattgc catttatttt
     9961 ccgctactct ttttttaaca aagtcttgat tttttggtac actatcctta tgaaaatcta
    10021 taaaacaata aaccacattg ctggtgaaaa tacttattac ctcgttaatg atcaagctgt
    10081 tatcctcatt gatccaggaa gtaacggcca agaaatcatt gctaaaatta aatcctttga
    10141 aaaaccttta gtggctatcc tcttaacgca tactcactat gatcatattt ttagtttaga
    10201 tttggtcaga gatgcttttg atcatccacc tgtttatgtt tcagaaaaag aagccgcttg
    10261 gctatcttcc ccagatgata atttatctgg gttaggccgt catgatgata tcattaatgt
    10321 tatagcaaga ccggcagaga acttttttaa attaaagcaa ccctatcaac taaatggttt
    10381 tgaattcaca gttttaccaa cacctggtca ttcttgggga ggagtctcat ttgttttcca
    10441 ctcagatgaa ttagtcgtta ctggcgatgc tctatttcgt gaaactatcg gtcgtactga
    10501 cttacctacc agtaactttg aggacctcat tactggtatt cgccaagaac tattcacctt
    10561 acctaatcat taccgagttt atcctgggca tggtccttct actaccattt gtcacgaaaa
    10621 aaatgctaac cccttttttc attgacatag ctattttaga tactgtttga ggttatccta
    10681 aaactgaaat catagtattt tactaaaaaa gagccactaa attttgaatg acaccccaaa
    10741 agttagatac atcaaacatc taacttttgg ggtgcttttc ttctcttgaa accttagaaa
    10801 ctgctatttt aaactgtatt gcctattaca aataaacaaa tgaatcaaaa caaaatgaaa
    10861 aggaccagtc ctgtgcaata caaaacgagg tccttggaat aatataagag gtccaacttt
    10921 tgagggtcgc tacgtttcgt cagcagcgct ttttttagaa ttttaaataa cgtctcatag
    10981 aaagcactga accaagcgaa ccaatcataa tacctatgac aaacaaagca ccaatcaggt
    11041 aatatacata aggatcaagc ggatacatcg agagattatt acgttgtaat tcttgtgcaa
    11101 aatgcttata aacaagatca tatccataat aaatcagaag agatggtaaa acagctccta
    11161 acaagccaac ccaagcccct tcaaaaaaga aaggccctcg aatataagaa tttttagcac
    11221 caactaaccg cataatttca atgtcgcgtt tacgagacat aatagtcata cgaatagtgt
    11281 ttgaaatgag aaagacagct acaaataata gcattgctgt tccgatcagc ccccaagttt
    11341 gaattaatgt tgaaaactta aatagtttat cggagttaat ccctccataa tctgcggctt
    11401 ctacgccttc aatcgttctg atctttttag tgatcgcctt aacttgttta ggcgtctggg
    11461 tctcaataag atagatatct tggagcggat tagtgtcttg gtcatacata ttccaaacat
    11521 cacctaaggt ttcttgcaat tttttcaatt gttcatcctt gctagaaaat gtaatctttt
    11581 tgacaccttt tatctgagca attttgtcgt aaacactgtg gtagttatcg ttattaacag
    11641 gttcaccggc agtattttga ataacttttg cagcatcagt tgaatcaact tgtaagtaag
    11701 tattgatatg gacattattt tcaacacccg aagcaacacg ctgtatattc aacaatgtcg
    11761 ccgcaaaaac accaactaaa gtcaatgtaa cagccaccat actaactgaa gcaaatgtca
    11821 tccaaaagtt gcgtttaaga tttttaatgg attcccaaat atgacggaaa aagtatctaa
    11881 tcatcgtaac cataatctcc tttttcttca tcacgtacta tgcgaccatc ttcaatagca
    11941 acaacacgat gacggaatgt attcacaata tgactgttgt gtgtcgccat taagatagtt
    12001 gttccctgaa cattaatacg ttcaagtaac tgcataattt cccatgaaat ctcaggatct
    12061 aaattccctg tcggttcatc tgcaatcaat aatttagggt tgttaacaat cgcacgcgca
    12121 atggccacac gttgctgctc ccctcctgat agctgacttg gaaaagaacg catcttatgt
    12181 ttcaagccaa ctaaatcgag tacctctggc acgcgttttt taatgtggcg gcgcttttca
    12241 ccaataactt ccatggcgta agctacattt tcaaagactg tttttcttgg tagcagtttg
    12301 taatcttgaa aaactactcc gatgtgacga cgaagaatcg gaacatcacg tgcttttagt
    12361 ttagttaaat tgaattcacc cacatatagc ttaccagttg tcagttgttc ttcacggtaa
    12421 aggagtttga taaaagttga tttaccagca cctgatgggc ctacgagata aacaaactca
    12481 ccttggttaa cagaaacatt gacgtctctg agggcggtcg ttgagcggcg gtatttttta
    12541 gtaacaccag acatttctat tagtgccatg tttctccttt attattttta gtcttcaatg
    12601 cgccacttaa ggtaggcatc aataaagcca ttgatttctc catccataac cttatctacc
    12661 tgagcaagct caaagttagt acgatgatcc ttcaccatcg tataaggggt gaaaacataa
    12721 gaacgaatct gacttcccca agtgatttct tttttatcac ccttgagagc atctacttct
    12781 tgtgctttct tttcttgttc taactgatat agcttagcct gcaacatctt catagcgcga
    12841 tcgcggtttc catattgagt acggtcaacg gtagacgata ccacaattcc agttggaata
    12901 tgcgtcaaac gaacacctgt tgatacttta ttgacattct gaccaccagc tcctccggaa
    12961 cggaaagtat ccattttaat gtcatcatca cgtacttcta cttcaatcgt atcatctaac
    13021 tctggcatca cttcaactga agcaaatgag gtatgacgac gtttagccga atcaaaaggt
    13081 gatatacgaa cgaggcgatg aactcccatt tctgatttca acaaaccata agcattagga
    13141 ccttcaaaag agagggtaac cgatttaata ccagcttcat ctcctgcttg ataatccaat
    13201 acttctactt tgaaaccatt agcattgcca aaacgagtat acattcgtaa gagtaaatct
    13261 ccccaatctt gcgcttcagt tcctcctgaa ccaggatgga tttctaagat agcattattg
    13321 tggtcatagg gttcagataa gagaagcgtc atttcataag cacccattat cttatctagc
    13381 tgcattagat tttcttctaa ttcttctttc aaagaatcat cttcgtcaag catctcaagt
    13441 aagagttctg tttcgtcaga taattcttgc atattatgga aagtatcata cttacctttt
    13501 aactcattta gttcttgaga tgtcttttgc gctgcaatat tgtcattcca aaagtctggc
    13561 tcagtcatgt gattttcaag aagagcaatc tcctcttcca aacgatctaa gtcaaagaga
    13621 cctcctgaag ctagtcaact tctctttatt ttctactatt ttttggcgaa tttcagccac
    13681 ttccatgaga tttctccaat ctttgttaat ctgttttatt ttatcataag gttcttaaaa
    13741 ataccataaa aggcttaaac ttgccattta tgacggatta attccaactc ttttcggcta
    13801 tcttcatttt ttaaggttaa atgactcata aacgccagta tctcatcgtt tggttttttg
    13861 acaatttctc ccaatgccca tatagctgtt gcagtatgaa tagggttatt atttttatca
    13921 ataatctcta ttaatttgac cactgcggtt ttatcatgag catttgctag cgcaataatg
    13981 gcattacgct gtaaaatatt tttacctcgc caacttccag caatcatacc aaatttttct
    14041 ttaaattgtc catttgataa actcaaaaat ggtattaatt ctggttgagc caactcagga
    14101 tcaatctctg tagctggtga attgtttata cccttgttat aaggacagca aatttggcaa
    14161 atatcacaac catagataac tgttttaatc ttttttcgaa actctatgtc catcattccc
    14221 ttatcttggg tttgaaatga tagacaacgc ttggcattca ttgagccatc accaattaaa
    14281 caagaagttg gacaggcatc tagacaacga cgacagtcac cacaatcata atcgacaggc
    14341 ttgtctggct cgatctctaa attggtaatt aattctccca aaaacatata agagccatat
    14401 tctttagaaa taactagacc attttttcct ataaaaccaa tacccgctct ttgggcaact
    14461 gcagtatcaa ctaaagctcc agtatcgacc attgcttttt gttgcaaagg aaaatctcga
    14521 cacagctctt caatgccttt tgatagtctg tctaactttt cgccgaccac ataatgataa
    14581 tcaagccccc acgaactagg tgtaattttt ccacgtttat aaggtgtttt ttgaggttgc
    14641 tgaggaagtt tgtgaggata agcaacgcca attgaaataa tcgttttagc cgattctaaa
    14701 tgcctttcag ggtagatacg atcctctatg actttatgtt caaatcctga attgcgcccc
    14761 tcttccacag aagcacgcaa tgacttttcc aaataatcaa agttatctgc cgtcgtaaaa
    14821 ccaattttag agataccaat ttctttggcg agtgccttaa tttctgcctt aatagtcata
    14881 ctactatcat aatcaaaata agttaagact tcaaggaaaa agaaggacaa ttctaatcaa
    14941 acataaccat tttacttcct gatttatttg gggtttctct cttaacatgc acaagaatgc
    15001 aacaagtaaa ttcaattaca tcgtaaaagt cccttttctg gttacctcta cttggtcaga
    15061 aatcacatct ccatcacgtc ttgtctagac agtcagctca gctctaaagt aagtctgact
    15121 ataagcaatg gacctacgaa gacaagcagc ataacacttc caaatattat tatacctaac
    15181 caaaaccgag tcacattacc cgtgcaactc atggactcag atactgcctt tgtcaaagca
    15241 gtattatgat ccttctatat ttactcacta gccaatctaa aaacaaatcc aaataatgac
    15301 cacggttaaa catatctcct gccacataaa gatggtcaac gactaacgat cgcatccaaa
    15361 tcacaaaaat cataatcctt ttctaggtga tcttatgcga cggttttttt gataataact
    15421 taaacatagt actcttttca gtgtcatatt gtaaataggt agctaaaaaa cagtaaaaac
    15481 acataaatat attcttgtta ttggtctgta gaaagtccta gacgtattct taagtgtctt
    15541 gaaatatcat caatgatcag ttagttgtgt tacttcacga tccaaaacaa ccgattttta
    15601 cacacagttg tcctaaatga tctatcttgt gatcagcttg actctgatca atgccatatc
    15661 ggtagtcggt aatggcaaaa actgtcagat tggctgcttt ggcggcagca atgccttttt
    15721 ggctgtcttc tactacaagc agttgttttt ttgtaatcct agtttttgta ctgctttatt
    15781 atagatatct ggataaggtt tgcctctaga aacatcttca cgcgctagga ttatttcaaa
    15841 ataatctttt atttgcgttg tctccaacgc caacaaaaca tcctgacgct tcgagtttga
    15901 tgccacagcc agtttaatac cttgtttttt caactgttca agacaagagt tcacttctgt
    15961 aatcaacagt ttttgataag gaggcttatg cgcttgtttg taagcgtcat agtcagttgt
    16021 aattgcctta acgatagcat catccctatt tttacctaac aactctttcc ataattcttg
    16081 aagattgccc ccaataaaat ctttagagtt caaatgatca atgggaattc cctttgtctt
    16141 aaaaaaatct tctcgtcgcc tcagataaaa aggttctgta tcaaataaaa caccgtccat
    16201 atcaaaaata attcctttaa tcatcaacat ttctcctttt ttctatttta gcataagttg
    16261 ctgtaaaagc tgtacagcta gtccattcta agtgatatca ctttagatca ggtccatact
    16321 aacgaattca aaaggtatta cttttgtttg atcataaaaa gtttatatcc tatatatcaa
    16381 acgacaggct catcttgtta aattagctgc tcttatatct atattatctg gaaaaacata
    16441 agcagaagca tattaattga atgaatgata aaaaaaacgt ttttactagc actaaagcca
    16501 aggtaaaaac gttttttaga ttattgtcgc aaacttttga tataatgagc ggctttctta
    16561 gtgattaggt tatattgttt ttggctcgct aactgattaa actcgcctcc tatgcctaga
    16621 acatctactc cagcagctag ccagtctttg agattatcta aagaaatccc acctgtaacc
    16681 ataacctcta cttgtggcaa aggagatttg attgcccgaa taaaggacat ccctacagta
    16741 gaaccaggaa aaattttgac catatcaacc cctgattcta atgctgtaac cacttcatta
    16801 accgtcatac aacctggtaa ataaggtatg ctataacgat gacaaatcaa agccactgct
    16861 cgattgaaat taggaccaac aataaactgt gctcctgcta aaatagcttg gcgggcagtg
    16921 acatcatcca aaactgtccc agcacctatc aacacctcag gatcttcttt gaagcgttca
    16981 gctaattgac cgataacctc actagcaaag gggttagtat aggtgacttc aattgtctta
    17041 atgccacctt caatactggc caggctgcaa gctaaagctt cctcactaga ctctcctcgt
    17101 acaaccagga caagccgatt agccttaagt ttggttaaga tttctatttt tcccatatca
    17161 tcacctctgg acatctcgtt gattagctaa gactgcttct acttgttgga cgctgtgata
    17221 gagctgatct tcagctactg tacattttag gctagctgtt gccacagcaa aatcaaggtt
    17281 gcgctgcttt tcgttacctt ctaacaactg ataaagtaaa cccgccacaa aagcatctcc
    17341 acttccaacc cgttgcaaga ctggggtcgt cacttcacag gattcgtaaa actgcccatc
    17401 ataggcataa gccttgaaac gattactgcc ttgggcatca tttgaacgct ccgtatggta
    17461 aatagcttgt aactgatagc gctgataaag tcctgccaga cgttctctaa tcatcgtagc
    17521 atctgcctta ttcctatcaa acatgtcaaa atcgtcactg tctaacagca aaggttccat
    17581 gccaaaaata atatccgcgt attgagcaaa gtgtgaaaag agtcgtttag catcagctac
    17641 cgcaatcata ctagatctaa agttaagatc aaaagaaaca caaatatccc ttttcttagc
    17701 ttctctcaca agtacctcaa tcaggtcctg agtttttttg cccagtgcca aactgatccc
    17761 actaaaatga aagtgactaa tgccttcaaa aatggcagct agatcaagac tatccttatc
    17821 taaggccgaa aagctagagc cacagcggtc ataataaacc tgactagaac gacaaccaaa
    17881 gccattttca aggtaataca gcccaacacg gttgccctgc caagccgtca aggtcatatc
    17941 cacaccgctt tgcttcaaaa actgatgaaa agcgtgacca acaggattat ctggtagggc
    18001 cgtaaatagc cttgcttcta agccaaaacc gcctagagtt cttgcaatat taacttctga
    18061 accgccgaaa aaaagctgag cgtcgcaggc attggtcaaa ggctgaaact gattgggact
    18121 aacgcgtatc agcggttcac caaccaataa taacttagac atgtgatctc ctttcctcgt
    18181 caaaatggaa ttactgttca agtactgatt tcaaataagt aactaattct gacggctccg
    18241 cattggcaaa gaagtactct ttaaaatgag cgccagaaat cgtttctttg aggaaatctt
    18301 ggtcgatgtc tttgacaatt gccaacaaat ctctgtaagt aatttttttc agatcactta
    18361 aaatacgtgc attccgctgt tcaggaatag ctcgcccttt agggtaacca ccacccattg
    18421 gttctgcaaa gaggcgttca aatattaagg tcaaattcaa ctctgctccc caaccaaaac
    18481 ctttggcaaa aggaatggac aaggcattac caccattgat ttgagaaaag agataagctt
    18541 ctgttggctc actcgcaaaa ccacaggtaa cacctgggaa gctgttcaag gccaacatag
    18601 ctccaacacc tgtcccacaa ccagtcacca caaaatcagc cgccttagtg gtcaataaaa
    18661 tagaagctaa gagtccattt tgaacatagg tcaactggct ttcaccttct gtgccataca
    18721 taccatagtt aaagacttgg tagccgtgtt tatctgtcac tgtggtaagg gcatcgtaaa
    18781 tgataccatt tttagccgct tggctatttt cattaattaa tgcaattttc atataaaatc
    18841 tctttctagt gtgtgattaa ggttgttttc caatataagc taaaatgcca ccgtcgacat
    18901 aaagaatatg gccgttgaca aaattactgg catcgctagc tagaaaaaca gctggaccag
    18961 ctaagtcttc tgttgtcccc caacgtgctg ctggtgtttt agcaatgata aattgatcaa
    19021 aaggatgacg actaccatct gcttgtcgct ctctcaacgg agctgtttgg ggcgtcgcaa
    19081 tgtagccagg accaatccca ttacattgaa tgttggcttc tccaaactca gaggctatat
    19141 ttttggttaa cattttcaag ccacctttag ccgccgcgta ggcactaact gtttcacgcc
    19201 ccaattcgct catcatagaa cagatattta taattttacc atgtcccttt gcaatcattg
    19261 atggtaatac agcttttgac acaataaaag gagcatttaa atcaatatca ataacttgac
    19321 gaaaatcttc tgcggccatt tcaagcatcg gcgtacgtct aataatacct gcattgttga
    19381 ccagaatatc aataacacct acttcatctt caatttgact aaccatctgt tggataccag
    19441 cttcatcagt cacgtcacaa acatagccat gcgcttctat ccctaactct cgataggcag
    19501 ccaacccctt atcaactaac tcttgtttga tgtcattaaa cacaatagtt gcacccgctt
    19561 gagcataggc tttggcaatt tcaaaaccaa taccgtagga tgctccggta atcaaggcaa
    19621 tcttaccttg taacgaaaac atattttcca taggattctc ctttttgtta ttaaattgag
    19681 taaacctgtt tactaagact ataacaaagt aaactagttt tgtcaagatt ttatgaaaac
    19741 ggttcaaaaa taaaaaagag aaactagcta gcttttaaaa ataggtttta ttccttaatg
    19801 gcatttcttt tacatagtgt tcttcattaa acaactatga ttgatccctt ttgacatgtt
    19861 atggagatta atctctattt tctctaaaaa taaatctcaa aaaagtattg acaaagaatc
    19921 ttgaaagcgc tacaatgttt ttaagtaaac cggtaaacta aaggagattt atttatgatt
    19981 gctattatcg ttatggggca tggacatttt gcgagtggta ttgtttcagc cttagaattg
    20041 atcgcaggca aacaagaaaa ggtcactgct attgatttta ctgctgagat gaccgccgct
    20101 gatgtgcagg accaattatc tcgtgcttta atcccagaag aggaaacctt agttctttgt
    20161 gaccttctag gaggtacccc ttttaaagtc gctgcaacct taatggaatc tcttccaaac
    20221 acgacgtgta atgttctttc tggccttaac ttagccatgc taattgaagc tagttttgca
    20281 agacaaacag cagcttcttt tgacgactta gtattaggac ttataacatg ttctaaagaa
    20341 ggcattgttg actggaaaac tctttctcag caagaagacg gtacgacaga cgatgagtta
    20401 ggaggcattt aatggcacga tctttaaaaa ccattgctct tgaacctatc aagcagcctg
    20461 aacgatttac caaagaagat ttcctaagcc aagaagacat tactcaggcc cttgacttag
    20521 ccctcaaaca agttcggctt aatatggact attttaaaga agactttcca acgccagcta
    20581 ccaaagataa ccaatatgcc attatggata atacagaatg gaccaatgcc ttttggacag
    20641 gttgcctttg gttagcttac gaatacagtg gagatgatgc cattaaagct ctggctcaag
    20701 caaatgacct ttctttccta gatcgagtaa caagagacat tgagctagat caccatgacc
    20761 ttggctttct ttacacccct tcttgcatgg ctgagtggaa acttttaaaa acttctgagg
    20821 ctagagaagc tgctttgaaa gcagctgaca aattagtcca acgctatcaa gacaaaggcg
    20881 gctttattca agcttggggg gtgctgggta aaaaagaaga ttaccgttta atcattgatt
    20941 gtctcctaaa catccaatta ctcttttttg ctagccaaga aactggagac aaccgttacc
    21001 gtgacatggc gatcaatcat ttctacgcct cagccaatca tgtcatccga gatgacgcct
    21061 ctgcttacca caccttctac tttgatccag aaacaggcga tcctgttaaa ggggtcaccc
    21121 gacaaggtta tagcgatgat tcggcttggg ccagagggca agcttggggc atttatggta
    21181 ttcctttgac ctatcgtttc ctaaaagaac ctgaactcat tcaactcttc aagggcatga
    21241 cccattattt cttgaaccgt cttcctaaag accaggtgtc ctactgggat ttaatttttg
    21301 gagatggtag cgagcagtct cgtgatagtt cagcaacagc cattgctgtg tgtggtattc
    21361 atgaaatgtt aaaaacctta ccagatcatg atcatgataa aaaaacatat gaagctgcca
    21421 tgcacagcat gctaagagcc ttgattaagg attatgctaa caaagatctc aatcctggcg
    21481 ctcctctgct acttcacgga gtctattctt ggcactctgg aaaaggggtt gacgagggaa
    21541 atatctgggg agactattac tatcttgaag ctctgcttcg tttctacaaa gactggaacc
    21601 cttactggta acaaacaccg atacctacat ttaaaaagga gattttatga cacaaccaaa
    21661 cattatcatg acgcgagttg atgagcgctt gattcacgga caggggcaat tatgggtcaa
    21721 atacctcaac tgcaacactg ttatcgtcgc taacgatgcg gtttctgaag ataagattca
    21781 acaatccctc atgaaaacag ttatcccatc cagtattgcc attcgttttt tctccattca
    21841 aaaagtgatt gacatcattc ataaagcaag tcctgctcaa agtatcttca tcgtggtcaa
    21901 agatttgcaa gatgccaaac acctagttga agggggtgtt cccatcacag aaatcaatat
    21961 cggtaatatt cataaaactg atgataaggt tgcgattacc cagtttattt cgctgggaga
    22021 aacagacaag tctgctattt gttgcttagc ccatgaccac caagtggttt ttaacaccaa
    22081 aacaactcca gctggcaata gcgcctctga cgttgacatc ttagattata tttaatgaat
    22141 aaaggagatt gatatggata tcaatttgtt acaggcactt ttaattggtc tttggacagc
    22201 cttttgtttc agcggaatgt tacttggcat ctacaccaac cgttgtatta ttctgtcttt
    22261 tggtgtcggg attatcttag gtgacttgcc aactgcactt agcatgggag ctatctccga
    22321 attagcttat atgggatttg gagtgggtgc cggtggtacc gttcctccta atcctatcgg
    22381 ccctggtatc tttggtacct tgatggctat taccagtgct ggtaaagtca ccccagaagc
    22441 ggccctagcc ttatcaacac caatcgcagt tgccatccaa ttccttcaga cattcgccta
    22501 taccgctttt gctggggctc ctgaaaccgc taaaaaacaa ttgcaaaaag gcaatattag
    22561 aggcttcaaa ttcgctgcta atgggaccat ttggtctttt gctatccttg gattaggact
    22621 tggtttatta ggtgccttgt caatggatac tctccttcac ttagtggatt atatcccacc
    22681 tgtcttgctc aatggattga ctgtcgctgg taaaatgtta cctgctatcg gatttgccat
    22741 gatcttatct gttatggcca agaaagaatt gattcctttt gtactaattg gttatgtttg
    22801 tgcagcctac ctccaaattc caaccattgg tatcgctatt attggtatca ttttcgcctt
    22861 gaatgaattt tacaacaaac ctaaacaagt cgatgcaaca actgtccaag gaggccaaca
    22921 agatgactgg atctaataaa ttaaccaaga aagattacct caaaacagct ctgagagctt
    22981 tcttcctaca aaatgggttt aattacagta attaccaagg tatcggatac gccaatgtca
    23041 tctatcctgc cttgaaaaaa cattttggca gtgataaaaa gggcttatat caagctttag
    23101 aagataactg tgagttttac aatactaacc ctcacttcct tcctttcatc accagtctgc
    23161 acttggtcat gttagaaaat gatcgtcctg aagaagaaac ccgaaacatc aaaatggcac
    23221 ttatggggcc tttagctggt atcggtgact ccttatcaca attttgcttg gcgccactct
    23281 tttcaacgat tgcagcttcc ttagctagtg atggtttagt tttaggtcca attttcttct
    23341 tcctagccat gaatattatc ctaactgcta ttaaaatcgg ttctggtctc tatggctata
    23401 aagtcggcac aagctttatt gataaattaa gtgaacaaat ggcggttgtg tcgcgcatgg
    23461 caaatattgt cggtgttacc gttatcgctg gattagctgc tacttctgtt aaaataactg
    23521 ttcccattac ttttgcggct ggcaaggtgg atgcggccaa tacagcacaa aaatttgtga
    23581 ctattcaagg aatgcttgat aaaattgctc ctgcactttt accagctctc tttaccttac
    23641 tcatgtatta ccttattaaa aacaaaaaat ggacaaccta caaattggtt ctcctcactg
    23701 ttcttatcgg tattatcggt agctggttag gtattttagc ttaatggttc acagagacct
    23761 gttatgagca tagggaaata gttcctaagc acatgatgag gtctcttttc ttttggaaag
    23821 gaaatgtcct atgtctcttg ccttcacacg ctttaaagaa acagtcaatc cagacttttg
    23881 caggaattac ctgctagact atcaggctga cagctatgct gatcgaaaaa ggatagctga
    23941 cctactctta accaatacct ttttatttga agataattgg gatatggagc cctgccacat
    24001 cccttaccac ttagatccca ttacttggca aaaggctgtc acagatgatc ctgagtggaa
    24061 ttttatgtta aatcgacaga cctatctcca aacattaatc ctcgtctacc ttgttgaaag
    24121 agatgaacgg tatctcctaa aagccaaaag tttcatcctc aattggattg aatcagctat
    24181 tcctttagac cctaaggggt tagctactag aaccttagat actggcatcc gttgttttgc
    24241 ttgggtcaaa tgtttgattt accttaatct ttttaacgcc ttaaccaaac aagaggaaag
    24301 cctcatccta gcttccctgg aaaaacaact tcaatttctc cataccaact accttgataa
    24361 atacagcctc agcaattggg gaatcttgca aaccactgcg attttattag ctgatgccta
    24421 ctttggttcc gaccttgata ttgctgcagc aacagctttc gctagaaaag aattgacgca
    24481 acaaatcgca ctccaaattc tcgaagacgg tagtcaattt gaacaatcaa ccatgtatca
    24541 tgtggaagta ctcaaagccc ttctggaatt aactgctcta gtccctgatt atcttccaca
    24601 gcttagacct actttgcttg ccatgagtga ttatctcctc aaaatgactg gtcctgacca
    24661 caaacagatc cccttgggag atagtgattt taccgatacc agagacattc taacactagc
    24721 agccactatc cttgaagaac cacacttaaa agctgctgct tttcccgcac ttgatatcga
    24781 tagtcttctc cttctaggag aaaaaggtgt ccacactttt gaacagttac ccgtccaaac
    24841 cttaccaact tttgcgcatc actttgaaca ttccgggcac attaccatca atcaagaaaa
    24901 ttattatctt tttttcaaaa atgggcccat cggtagttcc catacccata gcgaccaaaa
    24961 tagtatctgt ctctattata aaggacaacc acttttctgt gatgctgggc gatacactta
    25021 caaagaagaa ccattacgct atgccctaaa aagcgctagc catcattcta ccgccttctt
    25081 agaagaacaa ttacctgagc agattaatag ctcctgggct tacctttctt accccaaatc
    25141 caattattgt caccttcgac aaaatggaca cgtttacttt attgaaggta gctaccagac
    25201 gcaattctct gataggaaca actaccaaca tgaccgacaa attctcatct taccaccagg
    25261 aatgtttctt atcattgata cgatacaggc tcaaggcaac cactgtcttg tcagtcaatt
    25321 tattcttgat aatcacttag aagtaaagac agatcacctt agtgacctca gactaatcag
    25381 tgactgccct ttcactatcg aagaaacgat tttgtcaaaa aaatacaacc aatacctgac
    25441 tagtcacaaa ctcatcaaac gaaaaccctt taaagacaag ggttgcacca gtaccctatt
    25501 agtccctgac gataccaaag ttactccctt aacaccactt caaacaggca aacgcaaccc
    25561 aattgagacc gctcttgctt ggcatttaaa aggaaagcag tttgactaca gtatctgcgt
    25621 tctccaagag gacttaatta aaggagaaaa actagttctc ctaaatggtc ataaaattag
    25681 aggaaaggtg gttgttatca accatatcac gaatgagatt attcgattga aacactaaat
    25741 cttttacgag tccttatgtt ataatatgac taaccattac taaattaaag gagtgtatgc
    25801 aatgcagcgc aaagtaacca tcaaagatat tgctgaattg gctaaaactt ccaaaacgac
    25861 agtttccttt tacttgaacg gtaaatttga caagatgtca gaagaaacta aaaatcgcat
    25921 taaaagcacg attgaagcta ccaactataa accaagcatt gctgccagaa gtctcaatgc
    25981 aaaatcaact aaactcattg gcgtggttat tggtgatatt accaatagtt tttccaatca
    26041 aattgttaaa ggcattgaaa gcaaagccca ggaatttggt taccaaatta tcatcggaaa
    26101 cagcaactac gacccttctc gcgaagatga actcattgaa aagatgctga acctcggagt
    26161 ggatggcttt atcattcagc ctacctctaa tttcaggaaa tattctcgca ttatcgatat
    26221 taagaagaaa aaagtggtct tttttgatag ccaactttac gaacatcgca caaactgggt
    26281 caaaactaat aattatgatg ctgtttatga taccattcaa caatgtatcg ataaaggtta
    26341 tgaacatttt atcatgatca cgggtaaccc caatttactt agcactcgta ttgaacgcgc
    26401 ttcgggcttc attgatgtgt tagaagctaa tcatcttact catcaagaaa tgattattga
    26461 tgaaaatcag acttcttcag aggcaattgc tcagtttttg caaggatcac tgactaaaaa
    26521 gagtttggtt tttgtcccca attgttgggc tcttccaaaa gtatttacag ctatgaagtc
    26581 tcttaaattt aatataccag agattggact agtaggattt gataacattg agtggacaaa
    26641 attttcttca ccaacactaa caactattat ccaacccgcc tatgaagaag gcgaacaggc
    26701 tactaagatt ttaatcgatg atattgaagg tcacagtcaa gaagccaaac aacaaatttt
    26761 tgactgtcaa gtcaattggc aagaatcaac tttttaatgt caggcatacc aatagtcctt
    26821 atcgttgtaa cgattataaa aaaaggtttg agacaattgt ctcaaacctt ttagcgtcta
    26881 ttaaattgct ttttcatctt gaccaagtga tcgttgaaca gctttgacaa gctcaacaag
    26941 aacaaccatc aagagacttc cgataataac cactaaccat tgtgtgatcg tcaagtgtgt
    27001 cacatggaag aattgattaa atccaggaac aacaactgtt gccatcaatg ctacaaaggc
    27061 cactggaata gaatagttga acaatttgtt tttgaacaac ccaacagtaa agatggattg
    27121 gtaaacagat ttcacgttgt aggcatgtac caactgaatc aaaccgaggg tcacataagc
    27181 catggtcaaa gcatctgcat gaacatcatg gtagcttgtg tgttctggga acatcaaggc
    27241 aaaaccataa accccaagaa ccagcatggt ttggaaggca ccttggtaaa gaatggcttc
    27301 cttcacccca ccatcaaaga agcttgactt acgtccacga ggtttatgct tcatcacacc
    27361 aggctcagca ggttcaacac caagagcaat agctggtagg gtatccgtta ccaagttaat
    27421 ccaaagcaag tgaactggtt gaagcacatc ccaaccaaac aaggttgcaa ggaagatggt
    27481 aaagacttct gccatgttag cagaaagtaa atattgaatt gttttttgaa tatttgagaa
    27541 gaccttacgt ccttcttcaa ccgcaacgat aatggttgca aagttatcat cggcaagaac
    27601 catgtcagaa gctcccttag aaacctctgt acctgtaatt cccataccaa taccaatatc
    27661 tgccgtttta agagatggtg catcgttaac cccatcgcca gtcatggcaa caactttgcc
    27721 ttcattttgc caagccttaa cgatacgaac tttatgctca ggagatacac gggcatacac
    27781 ggagtattgt ttaaagactt tttggaactc ttcatctgat aattcgttga gttcagcacc
    27841 tgtaaagaca tgatcaacgc catcttcttc gataatacca agacgtttcg caatagcttt
    27901 tgctgtatct tggtggtcac cagtaatcat gattggacga atgccagctt ccttagctac
    27961 tttaacagcc tgtgctgctt caggacgttc agggtcaatc atccctacca aaccagagaa
    28021 gacaagatta gcctcaacaa tttctgtttc cagtgttgga agagcatcag aatatttgta
    28081 agccatcatc aagacacgaa gagcttgttt ggcaagactc ttattggtat caagaatggt
    28141 ttttttatca gcatccgtaa taggacgaat ttgtccattt tcttcaatct gtgtgacacg
    28201 cttcaacaat tggtctggcg cacctttaac agcgatgaaa tatttgccat cagcttgttt
    28261 atgaatggtt gacatcaact tacggtcaga atcaaatggt aattctgcca cacgaggttc
    28321 tgccaccata gcctcacgta cgtcaaaatt gtggtctaac ccaaactgta ccaaagctgt
    28381 ttctgttggg tcaccaatta acttccctga aggatcaact ttagtatcat tagcaaagtt
    28441 cattacacga agtgtcgtat tgtcaaaagc aatatcagct gatgaacttt ggagtgtccc
    28501 gttggtataa actttttcaa cggtcatttg attcattgtc aaggtacccg ttttatcaga
    28561 agcaatgatt tcagttgatc ctagcgtttc aacagctggt aatttacgga taatggcgtt
    28621 acgtttagca agtacttgag ttccaagaga aagcacaaca gtaacaatcg caggaagtcc
    28681 ctctggaata gctgcaacgg caagcgctac tgaagtcatc aagccttcaa gcgggtgttg
    28741 accacgcaag aagactccaa cagcaaaagt caccgctgca atcacaagga tcgcataagt
    28801 caaaatctta gaaaggttgt caaggttttg cttcaatggt gtatctgtct catcagcatt
    28861 agcaagcata ccagcaatat gcccaacctc ggtgtacatg ccagtgttag tgataacccc
    28921 aatcccacga ccatatgtca catttgagtt ttgatatccc atattgacgc gatcaccaat
    28981 accggcatct tctgaaacag ccgttgacaa gtctttttca accggtacag attctccagt
    29041 aagcgctgct tcttcgattt ttaaggagtt agcttccaaa agacgcagat cagcagggac
    29101 aacatctccc gcttctagca agacaatatc tccagggacc aattctttag agtctatctc
    29161 agtcacatgc ccatcacggc gaatacgagc cagtggactt gacattgatt tcaaggcttc
    29221 aatagcagct tcagcttgtc cttcttggta aacaccaaaa gcagcattta ggataacaac
    29281 agccaagata ataatggcgt ctgtcaaacc ttccatcccc tctgtaagaa ctgaaagaag
    29341 ggctgccaca atcaaaataa taatcatcaa atccttaaat tgatcaagga atttcataaa
    29401 taaacttcgt ttttcgcctt catcaagctc attacgacca tactctgcta agcgctcttt
    29461 tgcttgggca gacgtaagac cttcgcgact ggtctctaat tgagccagta cggtctcttc
    29521 actttgcgtg taaaacgctt catgtctttg ttctttagac aaattagtac ctccttggtc
    29581 tttttatttt agttattcaa ataaaaagac ctgtttattt tcttaaacaa gtctcgctgt
    29641 ttaagattgt accggaaacc gaaattccgt attgacgata caatcacggc tagccgtctg
    29701 ctactccctt gatataataa ccattatagt aaaaacctga ctaaaaaaca agaataatcc
    29761 ctaatcagat gatttactgc tatcttttta gttaatccca actcagctca agatgatagc
    29821 ttgcaaatag catttctgtt tgagcttgaa gcaaatgata atgaaggtcc aatttttgac
    29881 aagaacaatc taagtggtaa tgactggtat ctgtcaaaaa ttgttgaacc cccatcggcg
    29941 taggtagagc aatcaggacc ttcttgcccg caaaaaattt catgattgac tgagggttgg
    30001 aaaaacggct catggtcagt tctgtctcat tgtacttgat gacaaccttt tccttgtcag
    30061 tgttttgata gatgaggtac tgatagcccc ctttttctcg ccactcacaa tcatgaattt
    30121 cttgaataat ctctgtctca tctccaaagc gaatatggtt ggttaactgt aatttcataa
    30181 ctcttctttc tattctaagc tcgtctgttt attctatcaa tttctcctta ttttatcaat
    30241 ttttgatata ataaaaatta tgaatgaaaa agtatttcgt gaccctgttc acaactatat
    30301 ccacattgat aatccattaa tttatgacct tatcaatacc aaagaattcc aacgcttacg
    30361 acgcatcaaa caagtcccta caacagcttt caccttccac ggcgcagaac atagccgttt
    30421 ttctcactgc ctaggtgttt acgaaattgc ccgtcgggtg acagctattt ttgaagagaa
    30481 atacgctgat atctggaata aagatgaaag tctggtcact atgacggctg ccctcttaca
    30541 tgacattggt catggagcct attctcatac ctttgaggct ctttttcata cggatcacga
    30601 ggcctttact caggagataa ttaccaatcc tgagacagaa atcaatgcca ttttggtgcg
    30661 tcatgctcct gattttccag acaaagttgc cagcgtgatt aatcatactt atcctaacaa
    30721 gcaagttgtg caactgattt ccagccagat tgactgcgac cgtatggatt accttttaag
    30781 agattcttat ttttctgctg ccaattacgg tcagtttgat ttaatgcgta tcctacgtgt
    30841 gattcgacct gttgaagatg gcattgtctt tgaacatagt ggtatgcatg ctgtcgagga
    30901 ctatattgtc agtcgttttc aaatgtacat gcaggtctat tttcatcccg ccagccgtgc
    30961 tgttgagctc atcttacaaa atctcctcaa aagagcccaa cacttatatc ctgagcaaca
    31021 ggcttatttc caaaaaacag ctcctggact catccccttc tttgaaaaga aggctaattt
    31081 agcagattat atagccctag atgacggagt catgaatact tacttccaag tctggatggc
    31141 aagcgaagac catatcttat cagatttggc cagtcgcttt attaatcgaa aaattctgaa
    31201 gtctgttacc tttgaccaag attcccgagg agagttagaa cgcttgcgcc aattggtcga
    31261 atcagttggc tttgatccag attattacac cggtatccat atcaacttcg acctgcctta
    31321 tgatatttat cgaccagagc tcgaaaatcc aagaactcag attgaaatga tgcaaaaaga
    31381 cgggagcctt gctgaattat ctcagttatc tcctattgtc aaagcattaa cagggacaac
    31441 ctacggagac cgtcgctttt attttccaaa ggaaatgtta gagctcgatg acctctttgc
    31501 tcctagcaag gaaactttta tgagttatat ttccaatggg cattttcact ttagtcagta
    31561 aagcccttta agattgcaga aaaagtcttt tttgatactt ttcttaggtg gcacttatgg
    31621 cagcgagttc ctttggaatt ctatgcttta tttttgagcc tagggtctca aaaatcccgc
    31681 gtaacgtcac tgccagtatt gacaccactt ttgccacagc aaaaagcagc tgttttggct
    31741 cacgtcacac gcagaaataa gaaatcagtt gtcaaatcag cagagcagtt catgcttttt
    31801 gagcatcaaa ctgggtttgt ctccattcta agattgaaga aagagtactt tcttcaatct
    31861 ttttagttac tacagaggcg attgagctaa actaaaacgt tttctctcgt ttttccttaa
    31921 gtttcctttc ccatcccatg acaaaagcac ctatttttag taagatagga gggagaacat
    31981 ttttaagata aaccacaaaa aaacaaggag gatgccatgt ctatcaaact ggttgctgtc
    32041 gatattgatg gcacattact cactgatgac cgtcgaatta cagatgatgt ttttcaagct
    32101 gtccaagagg ctaaagccca aggtgttcat gtggtgattg ctactggcag acctattgcc
    32161 ggagtgattt cattactcga acaattagaa ctcaaccaca aaggaaacca cgttatcact
    32221 tttaatggtg gcttggtgca agatgccgag accggcgaag aaattgttaa agaattgatg
    32281 acttacgacg attatctgga aacggaattt ttgagccgaa aattaggagt tcacatgcat
    32341 gccattacca aagaaggtat ttacactgcc aatcggaata ttggaaaata cacagttcat
    32401 gaatcgactt tggtaaacat gcctattttc tatcgcactc ccgaagaaat gaccaataaa
    32461 gaaatcatca aaatgatgat gattgatgaa cctgaccttt tggacgcggc catcaagcaa
    32521 attcctcaac atttctttga taagtacacc atcgtcaagt caaccccttt ttatttagag
    32581 ttcatgccaa aaaccgtcag caagggtaat gccatcaaac acttagctaa aaaactcggg
    32641 ctagatatgt cacaaaccat ggccatcggg gatgctgaaa atgatcgtgc tatgttagaa
    32701 gtcgttgcca accctgttgt catggaaaat ggggttcctg aattgaaaaa aatagccaaa
    32761 tacatcacca aatccaacaa tgacagtggt gtggcacatg ctattcgcaa gtgggtatta
    32821 aactaatgta tacatataaa atcggaatat ctcctgaaga acatgatcag tttgtcttag
    32881 cacagccaca agctggctta cttcaaagtt caaagtgggg aaaagtcaaa gataactgga
    32941 aacatgaacg catcagcttt tatgaaaatg gggtacaagt tgctgctgct gcttgtttaa
    33001 tcagaaaact tcctcttggt ttcactatga tttatatccc acgtgggcca atcatggact
    33061 acgccaattt tgaactcctt gatttcgtta ttaaaacgct caaaactttc ggaaaaagca
    33121 aacgagcgct ttttgtcaaa attgatccaa gtttagtcat caaacaaact ttagaaggaa
    33181 aagaaagcaa agaaaatgat gtgacactct ctattattgc ttttctaaaa aaacttggtg
    33241 tcgaatggtc tggcagaaca aaagaactcg aagacactat tcaaccaaga atacaagcca
    33301 acatctacgc caaggatttt gactttgaca gcctgcccaa aaaagccaag caatccattc
    33361 gtaccgctac caataaaggt gttaatgtca cgattggtgg cagtgaattg ctagacgact
    33421 tttcagcctt gatgaaaaaa accgagaatc gaaaaggcat tatccttcga ggcaaatcct
    33481 attatcaaaa attactaggt atttatgctg gtcagtccta cattactatg gctagtttag
    33541 acttacctga acaaaaaaaa ctcttgatcc aacagttgga caaggcctta gcagaacaag
    33601 cacgattaac agataagagt aaacctagca aagtagcaga aaatcaaaaa accattgcac
    33661 ggctacaaaa agatttaacc attttaagcg agcagctcgc tgcaggcaaa acccgcatcc
    33721 cactagccgc caccctaacc ttgatttatg gcgagacttc tgaaaatctt tatgcgggaa
    33781 tggacgatga ttatcgcaat tatcaagctc ctcttttaac ctggtatgaa acggctaaag
    33841 aagcttttaa aagaggctgc cgctggcata atttaggagg tgtggaaaat caacaagatg
    33901 gtggactcta tcatttcaaa gctagactca accccaccat cgaagaattt gcaggtgaat
    33961 tcaacattcc tgtcggcctt gtttcaagct tagccatctt gacctataac ttaagaaaaa
    34021 aattaaggag cacaactaat ggcactaatt gaaatttcac aagaacaatt tgaccattac
    34081 tgccacagtc tcgtccatca ttcctttatc caaacctcag aaatggccag cttgatggct
    34141 aaaagagggg caaaaccaca atttcttgga cttgaaaaag atggtgaact aaaggttgct
    34201 gctatggtct ttagccaaaa agtggctgga ggttggcgta tggaactcaa tgcaggccct
    34261 aataccaatc atcccgaaga attagaacat ttttacactc aacttaaaga ctatgccaaa
    34321 caaaaagacg ttatcgaact cattttaaaa ccttacgaca attatcagtc atttgatact
    34381 gatggcatcc ctatttctca tccaaacact gaccttatta gccttttaac ggcgttagga
    34441 tataaacatg acggtttaaa aacaggctat cctgagggtg aacctgtttg gcattacgtt
    34501 aaaaagttag aaggaattga ttcctcacgt ttaacccatt ccttcagcaa aaaaggaaaa
    34561 gctttaataa aaaaagctaa tacttttggt attaaattac gtcaactgaa acgagatgag
    34621 ttacaccatt tcaaagaaat cactgaagca acctctgatc gtagagatta ccttgacaaa
    34681 tcattatctt actatcagga cttttatgat agttttggag actcttgtga atttatggtt
    34741 gcgactctta attttgaaga ttaccttaat aaccttaaac aaagacaact acaactagca
    34801 accagcatca acaaagttaa aggagacctt ggcaaaaatc ctcattctga aaaaaagcaa
    34861 aatagattaa aagaactatc cagccaattc gaaacctttc aggtaagaat ttctgaagct
    34921 ctccattttc ttgaagagta tggaactaaa gatgtgttcc ttgcgggaag tctttttatc
    34981 tataccgaac aagaggccgt ctacctcttt tcaggatctt accccaaatt taataaattt
    35041 tatagcccag ctctattaca agagcatgct atgctcaagg caattcataa gggcatcaaa
    35101 caatataatt ttcttggtat tactggaaag tttgatggtt cagacggtgt cttgcgcttt
    35161 aaacagaatt tcaatggctt tattttgcaa aaaccaggga catttagatg ttatccattt
    35221 ccaatcaaat atcatttcat tagattggca aaaaagctat taaaccgcta ttaagctaat
    35281 gcatttacct gtagaatcac ctttattctc tcatagtaag agttaagtca tctaaaaaag
    35341 aaaaggattt aaccttttct tttttagatg acttagttaa ggaaatcaag caaagcaagg
    35401 aagctatcag cttcaagtga tgcgccacct acaagggcac cgtcaacatc tggacaagcc
    35461 atgtaatctt taacgttttc aggttttaca gaaccaccgt attgaacgcg aactttatca
    35521 gcaacttctt gcccaaagtc tgctgctacc acgtcacgaa cagctttaca catgttttga
    35581 gcatcatctt gagtagctga tttaccagta ccgatagccc agattggctc ataagctaaa
    35641 acaagtgaag ctacttgctc tgctgaaaga cctgcaagtg ctgctgaaac ttgcgcacct
    35701 acaaactcaa cggctttacc agcttcataa gtttcaagag actcaccaca acaaacaatt
    35761 ggagttaaac cgttagcaaa gattgctttg gcttttttat taatgtcttc atctgtttca
    35821 tggaagtaat cacggcgttc tgagtgacca atcacaacat agtcagctcc catttcagca
    35881 agcacttttg gtgatgtttc accagtgaat gcccctgtgt tttcaaagta gcagttttgt
    35941 gcagcaactt taagaactga atctttagca gcttcaatag tggttaccaa atcaacagct
    36001 ggggctgcta cggcaacatc aacaaggtca gttgaaggta atttgcttgc tacagcctca
    36061 acgaatgctt ttgcttcttg agggtttttg ttcattttcc agttaccagc aataattggt
    36121 ttacgtgaca tatcacatac ctcttctttt tttatttata tcttctattt tagcacaaat
    36181 gaccgacgat taaaaggaat tgcttatatt ttaggaatat tttcacaaat aaaaactctt
    36241 tttctgaaag cttttcattc ttatttatca gcattttatg gagtttaaga tagcaaaaag
    36301 cccccgaatt gggggcttct tcaaacttaa gttgtctgac ttaaattgct attctgggaa
    36361 cttaattaaa attaagcttc gatttctgaa acgatacctg aaccaacagt acgtccacct
    36421 tcacgaattg agaaagtagt accttgttct acggcgattg ggtggatcaa ctcaacgttg
    36481 attgttacgt tatcaccagg cataaccatt tctgtacctg ctggaagttc gattgaacct
    36541 gttacgtcag ttgtacggaa gtagaattgt ggacggtagt tgttgaagaa tggagtgtga
    36601 cgtccacctt cgtctttaga aaggatatat acttcacctt tgaatttagt gtgtgggttg
    36661 attgaacctg gtttagcaat aacttgacca cgttcgattt cgtcacgttg aacaccacga
    36721 agaaggatac ctacgttgtc tcctgcaaga ccttcgtcaa gttgtttacg gaacatttca
    36781 acaccagtaa caacagcttt tttagtttct tctttgatac caacgatttc gatttcgtcg
    36841 ttgacacgaa cagtaccacg gtcgatacgt cctgaagcaa ctgtaccacg acctgtaatt
    36901 gagaatacat cttcgactgg aagaagcaat ggtttgtcag tgtcgcgttc tggttctgga
    36961 atgtatgaat caacagtatc catcaattcc atgatgatgt cttcaaattt agtgtcgcct
    37021 tcaagagctt taagagctga accttggata acgggaaggt catcacctgg gaaatcgtat
    37081 tctgaaagaa ggtcacgaat ttccatctca actaattcaa gcaactcttc gtcatcaaca
    37141 aggtcaactt tgttcatgaa cacgataagg tgtttaacac caacctgacg tgaaagaagg
    37201 atgtgctcac gcgtttgtgg cattggtcca tctgttgaag caactacaag gatagctccg
    37261 tccatttgag cggcaccagt gatcatgttt ttaacgtagt ccgcgtgtcc tggagcgtcg
    37321 atgtgcgcat agtgacgagt tgcagtttcg tactcaacgt gtgcagtgtt gatagtgatt
    37381 ccgcgttcgc gttcttctgg agcagcatcg atagaagcgt aatcttttgg ttggttaact
    37441 gatgaaggca agcgacgtgc caatacagtt gtgattgcag ctgttaaagt agttttacca
    37501 tggtcaacgt gtccgattgt accaatgtta acgtggggtt tactacgatc gtatttttct
    37561 tttgccattt taggaaaagc ctccaataaa atatatttta tagatagacg gtaggccata
    37621 cagtctaact ttaccttact attgtagcaa attacaatga aaatgcaagt attttatgtt
    37681 ttttgccatt tctttatttt ttaaatagaa gaaaaag
//
LOCUS       DATURV010000010        37032 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DATURV010000010 DATURV010000000
VERSION     DATURV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37032
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.010"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            263..1165
                     /locus_tag="VCF75_000497"
     CDS             263..1165
                     /locus_tag="VCF75_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP2546089.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSNISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
     gene            1178..1882
                     /locus_tag="VCF75_000498"
     CDS             1178..1882
                     /locus_tag="VCF75_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546090.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(2231..3196)
                     /locus_tag="VCF75_000499"
     CDS             complement(2231..3196)
                     /locus_tag="VCF75_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEP2546091.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            3268..4656
                     /locus_tag="VCF75_000500"
     CDS             3268..4656
                     /locus_tag="VCF75_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2546092.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGVAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDVEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            complement(4779..5231)
                     /locus_tag="VCF75_000501"
     CDS             complement(4779..5231)
                     /locus_tag="VCF75_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEP2546093.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            5462..6676
                     /locus_tag="VCF75_000502"
     CDS             5462..6676
                     /locus_tag="VCF75_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP2546094.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISMSLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            6893..7675
                     /gene="codY"
                     /locus_tag="VCF75_000503"
     CDS             6893..7675
                     /gene="codY"
                     /locus_tag="VCF75_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEP2546095.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDDEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            7822..8376
                     /locus_tag="VCF75_000504"
     CDS             7822..8376
                     /locus_tag="VCF75_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEP2546096.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            8820..9138
                     /locus_tag="VCF75_000505"
                     /pseudo
     CDS             8820..9138
                     /locus_tag="VCF75_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
     gene            9370..9672
                     /gene="gatC"
                     /locus_tag="VCF75_000506"
     CDS             9370..9672
                     /gene="gatC"
                     /locus_tag="VCF75_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEP2546097.1"
                     /translation="MKISEEEARHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDIAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            9672..11138
                     /gene="gatA"
                     /locus_tag="VCF75_000507"
     CDS             9672..11138
                     /gene="gatA"
                     /locus_tag="VCF75_000507"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEP2546098.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            11138..12577
                     /gene="gatB"
                     /locus_tag="VCF75_000508"
     CDS             11138..12577
                     /gene="gatB"
                     /locus_tag="VCF75_000508"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEP2546099.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGAIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            12574..12789
                     /locus_tag="VCF75_000509"
     CDS             12574..12789
                     /locus_tag="VCF75_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546100.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTAYKKTIIYAKI
                     RLTGIALEPSGVSDGYLMNHHVCPHYY"
     gene            13008..13247
                     /locus_tag="VCF75_000510"
     CDS             13008..13247
                     /locus_tag="VCF75_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP2546101.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIITGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            13639..14346
                     /locus_tag="VCF75_000511"
     CDS             13639..14346
                     /locus_tag="VCF75_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP2546102.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEERSDEI"
     gene            14336..15079
                     /locus_tag="VCF75_000512"
     CDS             14336..15079
                     /locus_tag="VCF75_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEP2546103.1"
                     /translation="MKYNSLNYLVQLLIVIVFLGGLYLFIKPEESVTPTQLNKKEIQQ
                     KDIKKTDRLRALPKVSVEDWELVLVNRDHITKEMSPELADINGISVDKRIEQATSDFL
                     AAAQAIDSQEHLISGYRSVDYQTELYQSSIKKEMANDPTLTQEAAEALVQTYSQPPGA
                     SEHHTGLAIDMSTVDTLNASDPSVAKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            15076..15645
                     /locus_tag="VCF75_000513"
     CDS             15076..15645
                     /locus_tag="VCF75_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP2546104.1"
                     /translation="MRKRLKFPYFLTLLACFLLLLVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            15780..16814
                     /gene="hrcA"
                     /locus_tag="VCF75_000514"
     CDS             15780..16814
                     /gene="hrcA"
                     /locus_tag="VCF75_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEP2546105.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKAFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDNSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            16817..17389
                     /gene="grpE"
                     /locus_tag="VCF75_000515"
     CDS             16817..17389
                     /gene="grpE"
                     /locus_tag="VCF75_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEP2546106.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            17570..19396
                     /gene="dnaK"
                     /locus_tag="VCF75_000516"
     CDS             17570..19396
                     /gene="dnaK"
                     /locus_tag="VCF75_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEP2546107.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            19677..20813
                     /gene="dnaJ"
                     /locus_tag="VCF75_000517"
     CDS             19677..20813
                     /gene="dnaJ"
                     /locus_tag="VCF75_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEP2546108.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            21033..21824
                     /locus_tag="VCF75_000518"
     CDS             21033..21824
                     /locus_tag="VCF75_000518"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEP2546109.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            21898..22332
                     /locus_tag="VCF75_000519"
     CDS             21898..22332
                     /locus_tag="VCF75_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP2546110.1"
                     /translation="MEYDKINHYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            22333..23307
                     /locus_tag="VCF75_000520"
     CDS             22333..23307
                     /locus_tag="VCF75_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEP2546111.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLANIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVRI"
     gene            23368..23592
                     /locus_tag="VCF75_000521"
     CDS             23368..23592
                     /locus_tag="VCF75_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP2546112.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            23794..24765
                     /gene="fabK"
                     /locus_tag="VCF75_000522"
     CDS             23794..24765
                     /gene="fabK"
                     /locus_tag="VCF75_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEP2546113.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            24790..25728
                     /gene="fabD"
                     /locus_tag="VCF75_000523"
     CDS             24790..25728
                     /gene="fabD"
                     /locus_tag="VCF75_000523"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEP2546114.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            25718..26452
                     /gene="fabG"
                     /locus_tag="VCF75_000524"
     CDS             25718..26452
                     /gene="fabG"
                     /locus_tag="VCF75_000524"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEP2546115.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            26467..27699
                     /gene="fabF"
                     /locus_tag="VCF75_000525"
     CDS             26467..27699
                     /gene="fabF"
                     /locus_tag="VCF75_000525"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEP2546116.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMIDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            27701..28201
                     /locus_tag="VCF75_000526"
     CDS             27701..28201
                     /locus_tag="VCF75_000526"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEP2546117.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            28198..28620
                     /gene="fabZ"
                     /locus_tag="VCF75_000527"
     CDS             28198..28620
                     /gene="fabZ"
                     /locus_tag="VCF75_000527"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEP2546118.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            28652..30016
                     /locus_tag="VCF75_000528"
     CDS             28652..30016
                     /locus_tag="VCF75_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEP2546119.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAY
                     GNIIHLGERDCSLQRHNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            30025..30891
                     /locus_tag="VCF75_000529"
     CDS             30025..30891
                     /locus_tag="VCF75_000529"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEP2546120.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            30888..31658
                     /locus_tag="VCF75_000530"
     CDS             30888..31658
                     /locus_tag="VCF75_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEP2546121.1"
                     /translation="MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYKRFRKY"
     gene            complement(31881..33158)
                     /gene="serS"
                     /locus_tag="VCF75_000531"
     CDS             complement(31881..33158)
                     /gene="serS"
                     /locus_tag="VCF75_000531"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEP2546122.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(33550..33909)
                     /locus_tag="VCF75_000532"
     CDS             complement(33550..33909)
                     /locus_tag="VCF75_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEP2546123.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGMGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(34023..34934)
                     /locus_tag="VCF75_000533"
     CDS             complement(34023..34934)
                     /locus_tag="VCF75_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP2546124.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(34951..35760)
                     /locus_tag="VCF75_000534"
     CDS             complement(34951..35760)
                     /locus_tag="VCF75_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEP2546125.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGIIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(35849..36841)
                     /gene="manX"
                     /locus_tag="VCF75_000535"
     CDS             complement(35849..36841)
                     /gene="manX"
                     /locus_tag="VCF75_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEP2546126.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
BASE COUNT        11435 a         6508 c         8007 g        11082 t
ORIGIN      
        1 tagttagttt tgagataaac tgttataaag tataaagagg tcactgtttc tggccttttt
       61 tacttatgct ttacgatgag actgctatgt gaagcagcat taacccattt tcagtataac
      121 ttttttattg ctaaaataat catattatta aaattcttat ttatattttt ttaataaaaa
      181 gaagaacttg tcacaaccgt ttacagatgt tattatgtgt aagatattgt attaatcgat
      241 aagtacaaaa aaggagcgga aaatgacaca gcaatcaatg ttactaagta ttgagcaagt
      301 aaataaatcc tatggtaaaa atcaggtttt aagtaatatc tcttttgata tttacaaagg
      361 agaaatttgc ggccttgtgg gtcaaaatgg tgctggaaag acaacgttaa tgcgtatttt
      421 atctggccta attggaaaag attcagggca aatcaaacag ttacaaccct atcgaatggg
      481 atcaattatt gaatcaccga cactttatcc caatatgaca gctcatgata atctttacta
      541 tgcagcgttg caattgagat tagctgatgc aaaagagcgc atacatgagg ttttggaact
      601 tattgggtta gaaaaagtga gcaaaaagaa aaaggtgaaa gattattctt tggggatgcg
      661 ccaacgtttg gctattggtc tttcgatact agattttcca gaatttttaa tcttagatga
      721 acccattaat ggtcttgatc cagcaggaat caaagaaatg cgtcagatta ttttgaacct
      781 aagagattgc tatggtatta cgattttgat ttccagtcat attttatcag aattagactt
      841 agttgttgat cgttacgtga ttatgcataa aggcaaagtt attaagagta tggacaaagc
      901 agagcttaaa gctcaagtaa aagtacaaat tgcccttcat acaagcgatg atcagctggt
      961 aaaaaataga cttttagaac tggggcttag agttgagact gatggtcaga tgctactaat
     1021 aaaaccaact ttatcagtaa tggagcttat caaaattgtg ctagatctgc ctgttgagat
     1081 ttttgacatt tatcatcatc aagtgagctt tgaacattat tacctcgatt tgttaggtaa
     1141 agatgcagcg tcaccattaa tttagaaaga ggtttttatg atttcctatc ttaaagcaga
     1201 tttatttcgg atacgccaag agcgtttagc tctattttct tgtatgctac ttgttgtcat
     1261 tgctgttggt gtagcctata gttttcgcaa gacacctaac caagtcatca tgatacaaat
     1321 cattaatatt attactgatt ttgtgacgct tttcttttta acaccagccc gtatcttttt
     1381 tagtgatgac tataactacc gtacgattaa taatgttgtg gtcaaacaac aaaatcgtac
     1441 tttaattata atctataaac tgctagttag ttttggctta tctcttttat ttattagttt
     1501 aacttatctt cttagtgctt tagcatttca tttatttact ggtcaagcat actatggaat
     1561 agtcatagat cggatgatta aacaactgcc tttttatatt gctattattg ctttgtgtca
     1621 atttttgttt aatatacttc gtaaaactta tcagtcaatc acagcttata tactttatgt
     1681 ccttcttttt gataaagtat ctgctacttt agcaaaccat ttttttaagt tagatattag
     1741 cccagttttg atgtttaaac acctatcctt gtctcttggt gataaacacc tttctagtac
     1801 tagcatatta gtggcctttg gtttaactct ttgttatctt ttaggcaatg tctttctttt
     1861 ttccaaacga gaactaaaat aaagggtaat atgtgctttg tttattttaa aagacaaaga
     1921 agttcttaga aaaaggtcgt cttcatcatt actaaaaaag gatgagcaag tagttttgtt
     1981 attattcaag taagctcata cttaatgaga taaaataaag acttgtttac ccggtatctg
     2041 attgctaaga gttggattta ttggaatact ggtctcaaac tgttatagaa atgattatga
     2101 gtgcacaatc tcatagtttg aaacatagtt gtttattata gacatttagg agatgtccta
     2161 atttaaagct taaagtacta gaaatgtccc actaatatgt tttagtggga catttctaca
     2221 ggaagctaga ttatccttca atataatctt ttagctcttg accagtaagt ccggcgttga
     2281 gagcaattaa taactttaga cgggcttttg gagcattcaa ttcttttaca aacataacac
     2341 ctgcttcttg tagcatcgca ccaccacctt cataagcata gacaggttct gccataccat
     2401 tgaaacaacg ggaaacaaga acgacaggaa tacctaaagt aattaagtgc tcgatctccc
     2461 caactgctaa tggtgggaca tttcctgccc ctagagcttc gataactaga ccttgaatgc
     2521 tacctggagt taatagactc aagatactac catcgcccat acctgcgtat gctttgataa
     2581 tggggatggt tccagaaatg catctcaagt caaaacgaat acgaggttca gcagttttga
     2641 aaaaaagtag gtcatttttc atgatgatac caagcggtcc gtgtgtgggt gtttggaaag
     2701 ttgaaatatt ggttgtatgt gttttagtaa catatttagc agcatggatt tcatcgttca
     2761 taacaacaag tacccctttt tctttagctt tatcactact tgcaactcta agtgcggtaa
     2821 ggtaattata tatgccatca ctaccaactt cattagaaga cctcatagca cctgttagga
     2881 caacaggtat attagtaggc aatgccatgg tatctaaaaa ataagctgtt tcttctaagg
     2941 tatctgttcc atgtgtgatg actataccat caaagacatc agttctttct tgaatatgat
     3001 gataaataga taacatgtgg tgaggcgtga tgtgaggact cggaagattt agaaaatcac
     3061 taacggtaag ctgaatatct tgggctgcag catgtatttt cgtcatagga ttatcttgat
     3121 ttggaacaac cctaccagag ttatcagctt gcatagaaat ggttccacca gtgtgtaaga
     3181 ctaggatttt tttcatttga ttgtcaaact tccttttatt tatgatataa tctattctat
     3241 cataaacaca ggaggcaaaa gaaggttttg acagtaaaag cagtcttttt tgatattgat
     3301 gggacccttc tcaatgatcg taaaaatatc caaaaaacaa cgcaaaaagc catccaacaa
     3361 ctcaaaaaac aaggcattat ggtcggttta gcaactggtc gtggaccggg atttgtgcaa
     3421 ccttttctag aaaattttgg attagatttt gccgttactt acaatgggca atatatctta
     3481 acaagagata aggtcttata tcagaatcaa ttgccaaagt cgatgattta caaagtgatt
     3541 cgctatgcca acgaaaaaaa gcgagaaatt tctcttggaa cagcttctgg attagcagga
     3601 tcgcgtatta ttgatatggg gactagccca tttggacaag ttattagtag ttttgtgccc
     3661 aaaagttggg caagaacagt tgagggtagt tttaagcatt tgattcgccg aatcaagcct
     3721 cagagtttca gaaatttagt aactattatg cgggagccta tttatcaggt tgttctggta
     3781 gcgtcacaag ctgaaactaa gaaaatacaa gagaagttcc ctcatataaa aattacaaga
     3841 agtagtcctt attccttgga tctgatctct gttgatcaat caaaaataaa aggaattgaa
     3901 cgtttgggag agatgtttgg atttgacttg tctgaagtga tggcttttgg agattcagat
     3961 aatgaccttg aaatgttatc aggagttggt atcggtgttg ctatgggaaa tgctgaaact
     4021 gtggtaaaag atggagccca tttcactacc gactctaata ataatgatgg gatttccaaa
     4081 gccttagctc actacggtct catccatttt gatgtagaga agagctttaa aagccgtgat
     4141 gaaaatttta ataaagtcaa agattttcat cgtctgatgg actcagatac tattgaaaca
     4201 ccgcgtagct ataccatttc agaagctggt tataggtcag gcttcaaagt agaagaaatt
     4261 gttgagtttc tttacgcagc aagcaaggga gaccaacaac aatttacaca ggctattttc
     4321 gatttgcatg gtgctgttga ccaagcagct aataaggtac aagctaaaaa acatgttgaa
     4381 acgcccctga ttgggcaagt tgatgcctta gcggatttgc tttattttac ttatggttct
     4441 tttgtactga tgggagtaga tccacaacct atatttgaag cagtacatga agcaaatatg
     4501 gctaaaattt ttccagatgg caaggcacac tttgaccccg ttacccataa aattcaaaaa
     4561 cctgactatt ggcaagagcg tcacgcacct gaagtagcca taaaaaaaga gttagacaaa
     4621 caattacaaa aatcactaca acgattcgat aaatagaggt ttactctttt aaatatcaag
     4681 aaataaaaag atattgacta gccttagctc agcacaaaaa aacaagcaat ttcgttgtag
     4741 atgctgcttg ttttttggtg tttgaaaatt gtaatagact aaagtgtttt agtgctatct
     4801 cggactacaa gtaagtcaat cttagcgtgt cgcataatgt actcagaaga tgagccaata
     4861 aggaggcgtt caaaggtatt aagaccagta gctcctacca taattaaatc agcgttctca
     4921 cgatctggaa tatcgtgagc tagtaagttt ttgggattgc caaattcaat aatttgtttg
     4981 atgtttgtaa ttcctgctat ctgtgcttgt ttttcaaaat catctaaaac gtccttagct
     5041 tcttgttcta atttttcata gatgtaagta tcaaaagtag ctacgctttg caaggcacgg
     5101 gtgtcaataa cgtgtactag tagtaaggta gcgtcgttac gtagtgctac gttaacgcct
     5161 ttattgaaag ctaattcaga ctcataagag ccatcaatag caacgaggat acgtttatat
     5221 tttagtgaca tagaactgtc ctcctttaac agtttgacaa gaatagcttt tattcttttt
     5281 ttcattatac tcttttttga gtcattttga aagcgatcca ttattttggc taaactaaag
     5341 actgtattat cgtctcataa gacctgtgtt aaaacgatta gctgtcctaa gctttgatgg
     5401 ataaattctt tctattttaa gtattttggt ttataatatg gaatacgagg tgtatgagac
     5461 tatgaaaatt attgaaaaat catcaaaatt agaacatgtt gcctatgata ttcgagggcc
     5521 tgttttggat gaagctaatc gcatgattgc gagtggtgaa aaaattctgc gtttaaatac
     5581 tggcaatcct gctgcctttg gttttgaagc accagatgaa gtgattcgtg atttaattgt
     5641 caacgcgagg ctaagtgagg gctattctga cagtaaaggg attttttcag cgcgtaaggc
     5701 tatcatgcaa tattgccaac tcaagggctt cccagatgtt gacattgaag atatttattt
     5761 gggaaatggc gtgtcagagt tgatatccat gtcccttcaa gctttgctag ataatggcga
     5821 tgaagtttta gtaccaatgc cggattatcc tttatggacg gcttgtgtga gtcttggtgg
     5881 aggaaaagcg gtccattatc tttgtgatga agaagctggt tggtatcctg atattgctga
     5941 tatcaaatca aaaatcacga gtcgcaccaa agctattgtg gtcatcaatc ctaataaccc
     6001 aacaggagcc ctttatccta aggagatttt agaagacatc gttgctttgg caagagaaca
     6061 ccagctgatt atttttgccg atgaaatcta tgatcgtttg gtgatggatg gtaaagagca
     6121 tattgctatt gcaagtttgg cacctgatgt tttttgtgta tctatgaatg gtctttccaa
     6181 atctcatcgt attgctggtt ttcgtgttgg atggatggtc ttatcaggtc ctaaagacca
     6241 tgttaaaggt tacattgaag ggttaaacat gcttgccaat atgcggttat gctccaatgt
     6301 tttagcccaa caagttgttc aaacttcctt aggaggtcat caatcagttg atgcactgtt
     6361 actgccagga gggcgtattt ttgagcaacg taattttatt tatgaagcta ttaatgctat
     6421 ccctggctta tcggcagtta aacctgaagc aggtctttat ttatttccga aaattgatcg
     6481 tcagatgtat cgtattgatg atgatgaaga atttgtctta cagttattaa agcaagaaaa
     6541 agtgatgttg gtacatggac gtggttttaa ctggaaagat ccagatcact tccgtattgt
     6601 ttacctacca agtgtagaag aattagaaga tgtccaagaa aagattacac gtgtcttata
     6661 ccagtataga cgttaatgca atgctcatct ttgattaata aaaataacgt ttcttaaaag
     6721 aatgtgaccc tatgctctaa caagctagtg cttatctcct ttaaatgaag tgataataag
     6781 gcatcaatag ttggaattgt agtaagaata gaaaattttc tgataattcc ttgcaaaagc
     6841 gagttagctt tgttataatg aaagcgcaaa taattcaaag gaaaaggaaa aaatgcctaa
     6901 cttattagaa aaaactcgta aaatcacatc tattttgcag cgttccgtag atagcctaga
     6961 aacagaatta ccgtataaca caatggcatc tcgcctagca gatatcattg actgcaatgc
     7021 ttgtattatc aatggcggcg gcacactgct tggttatgcc atgaaatata aaactaacac
     7081 tgatcgtgtt gaggaatttt ttgaagctaa acaatttcca gatacttatg taaaggctgc
     7141 tagccgagtt tacgatacag aagctaacct ttctgtcgaa aatgaattga ctatattccc
     7201 tgttgagtct aaagacactt atccaggagg tctaacgact attgcgccga tttatggtgg
     7261 agggatgcgc cttggatcac tcattatctg gcgtaatgac gatgagttta gtgatgatga
     7321 tttgattttg gttgagatct caagtactgt tgtggggatt caactattaa atcttcagac
     7381 agaaaactta gaagacacca tccgtaaaca aacagcggtc aacatggcaa ttaatacgct
     7441 ttcttattca gaaatgaaag ctgttgcagc aattcttgga gagttagatg gtaatgaagg
     7501 acgattgaca gcttctgtta ttgctgatcg tattggtatt acccgttctg ttattgtcaa
     7561 tgctctgcgt aaactagaaa gtgcagggat tattgaaagt cgttctcttg gtatgaaagg
     7621 gacatacctc aaagttatca acgaaggtat ttttgctaaa ttaaaagaat tttaaataga
     7681 aaaagacaac atctcttttc aagagatgtt ttttgctgtt aatggtagat gcaaatagat
     7741 gtcttgatac ctgtaataaa aactgataca atgccatata taagagataa ggggagaggt
     7801 ttccctgttt aggagatagt gatgagagca ttgatttcaa ttgattacac aaatgatttt
     7861 gtagcagatg atggtaaatt aagtgcaggt aaatctgccc aggctattgc cactaaaata
     7921 gcagaggtaa ctaaaacggc atttgatcaa ggagactata ttttttttgc tattgattgt
     7981 catgatcaaa atgatccttg gcatcctgag agtaagttat ttgcagcaca taatatcaaa
     8041 ggaactactg gacgccatct ttatggtcca ctagctgaag tttacagtta tatgaagcaa
     8101 catcccagag ttttttggat tgataagcgt tattattcag ctttttcggg gactgattta
     8161 gatattagac tacgagaaag aggtatcact caattagtgt taacaggagt tttatctgat
     8221 atctgtgtct tacatactgc tattgacgct tatcatcttg gttatcaatt agagattgtt
     8281 aaatcggctg tcgctagcct tacaaaagaa tcttatgagt ggtctttggc tcactttgag
     8341 caggttcttg gtgctaagct tattaacgat tactaaaaga tcctaccaaa ctattgattc
     8401 agtgactaag agtttggtaa ccgtaatcat tgctctatgt ggttttaccg tatgtatggg
     8461 ttaggtagat gaggtatgtg acatgctggt cagaattagt cctgatgatt cttgtgctgt
     8521 ctaaaaggca actaaacttt ggactgtgtc agatagaaca catgtttttt tgaagaagac
     8581 aggattttag ctgtttgaga aatgatttga gttgatggtt tagaggatcg tctcacagcc
     8641 attagacaaa ctctagtctt ttcagcatta gaatataaat aaagcttcaa actcttgaat
     8701 gctagaggct gtaccatttt tatcttaggc ttatcacatc acatgttgtc acaatcgttg
     8761 aggatttggt aaattaggat aaagcacccc tttattacct tcgtaaacat attaccgact
     8821 tgaaagaacg ttactttttc tgtgatgttg taggaaaatg cttggaagaa tacgttgaca
     8881 ggggtatttt tgataaggag ccgttccaga cctttgatca gaaagggatt ggccgtctct
     8941 ttagcccgtt cggttaagcc tgagttgaaa cttggtattt gtggggaaca tggtggcgat
     9001 cctgcttcca ttgactttta ccacagccaa ggcctgacct acgtttcttg ttcgccattt
     9061 agagtgccgc ttactcgctt ggcggctgct caggctgcca tcaaagcttc aggccacagt
     9121 cttacccaag acaaatagag tttagtttgt cacacttcct gactaaggtt ttctgacctt
     9181 agtcaggaag ttttttgctg tatagctttc tagaattgca ttagtcacct atggtaaatg
     9241 ggcgcaattg tgatataata attgagacta tgtaaaaacg atgtgaggag tttttccgct
     9301 acctttggag aatagcagag ctgttctttg gaattggaat acttctgatc atcttggatg
     9361 ggagaaatca tgaaaatttc tgaagaagaa gctcgccatg tcgcgaagct atcaaaatta
     9421 tcattctcag aaagtgagac aacaaccttt gcaacgacct tatccaaaat cgttgatatg
     9481 gttgaattgc tgaacgaagt tgacaccgaa ggtgtcgcca ttacaacaac catggcagat
     9541 aagaaaaatg tgatgcgtca agatattgct gaggaaggaa cagaccgtgc cttgctcttt
     9601 aaaaatgttc ctgaaaaaga aaatcacttt atcaaggtac ctgctatttt ggatgacgga
     9661 ggagatgcct aatgtctttt aatcataaaa ccattgaaga gttgcatgac ctccttgtag
     9721 ctaaggagat ttccgcaacc gaattgactc aaaaaacact tgaagacatc aagtctcgtg
     9781 aggaggctgt cgggtcgttt atcactgttt cagaagaagc tgctcttaaa caagctgcgg
     9841 caattgatgc caaaggtatt gatgctgata acctcatgtc gggtattcca ctagctgtta
     9901 aggataatat ctcaaccaag ggcatcctaa ccacagccgc ttcaaaaatg ttgtataact
     9961 acgagccgat ttttgatgcc acatcagtag caaatgccta cgctaaagac atgattgtca
    10021 ttggaaaaac caacatggac gaatttgcca tgggtggttc aacagaaacc tcttatttca
    10081 agaaaacgaa aaatgcttgg gaccacacaa aagtacctgg tggctcatca ggtggttcgg
    10141 caacagcagt cgcttcagga caagtccgtt tatctcttgg ttcagacact ggtggttcta
    10201 ttcggcagcc agctgcattt aatggtgttg tcggtttgaa accaacctat ggcactgttt
    10261 ctcgctatgg tctcattgct tttggaagtt cacttgatca gattggacct tttgctccga
    10321 cagttaaaga aaacgcccaa ttattaaatg tgattgcaag tagcgatgtc aaggatgcca
    10381 cttcagcgcc tgtccgcatt gcagactaca catctaagat tggtcgtgac attaaaggca
    10441 tgaagattgc ccttcctaaa gagtatcttg gcgaagggat tgatcctgag attaaggaaa
    10501 cggttcttgc ggcagctaaa caatttgaag ccttaggagc tactgttgag gaagttagtc
    10561 ttccacatag caaatatggt gtggctgttt attatattat tgcttcgtca gaagcgtctt
    10621 caaacttaca acgttttgat ggcattcgct atggtttccg tgctgacgat gctaaaaact
    10681 tggatgaaat ctatgttaac actcgtagcc aaggctttgg tgatgaggtg aaacgtcgta
    10741 tcatgcttgg gacctttagt ttgtcatcag gttattatga cgcttatttc aaaaaggccg
    10801 gtcaagtgcg taccttgatt attgaagatt ttgacaaggt ctttgcggat tatgatctta
    10861 ttctaggccc aacaacaccg acggttgctt ttggcttaga cacactcaac cacgatcctg
    10921 ttgccatgta tttggccgac ttattgacca ttccagttaa cttggctggt ctgccaggaa
    10981 tttccattcc agcaggattc gtcgatggct tgccagtagg gcttcaatta attggtccga
    11041 aatacgctga agaaaccatc tatcaagcag cagcagcctt tgaagcggtg acagattatc
    11101 acaagcaaca accgattatt tttggaggtg acaagtaatg aattttgaaa ccattattgg
    11161 gcttgaagtc catgtggaat taaataccaa ttccaaaatt ttctcacctt cgtcagctca
    11221 ctttggtgag gatccaaatg ccaacacgaa tgtgattgat tggtctttcc caggggtctt
    11281 accagtcatg aacaaagggg tgattgatgc gggcataaaa gctgcgcttg ccctcaatat
    11341 ggacatccat aaagagatgc atttcgaccg caaaaattat ttctacccag ataatcctaa
    11401 agcttatcaa atttcacagt ttgacgagcc gattggctac aacggatgga ttgagattaa
    11461 attggaagac ggttcaacca agaaaattcg tattgaacgc gcacacttgg aagaagatgc
    11521 tggtaaaaac actcacggca cagatggtta ttcttatgtg gaccttaacc gccaaggagt
    11581 tcctttgatt gagattgtgt cagaagccga catgcgttct cctgaagaag cctatgctta
    11641 tttgacagct ctgaaagaaa tcatccagta cacaggcatt tctgacgtca agatggaaga
    11701 aggctctatg cgggtggatg ccaatatttc ccttcgtcct tacgggcaag aacaatttgg
    11761 aacaaagact gaattgaaaa acttgaactc cttctcaaat gtccgtaaag ggcttgaatt
    11821 tgaggtggaa cgtcaagcca aactcttgcg ttcaggtggg gcgatacgcc aagaaacgcg
    11881 ccgctatgac gaagcgaata aggggactat tttgatgcgg gtcaaagaag gggcagcgga
    11941 ctatcgttat ttcccagaac cagacctacc cttgtatgag attgatgatg cttggattga
    12001 cgagatgcgt gctcaattac ctcaattccc agctcaacgt cgtgctaaat acgaagagga
    12061 acttggtctc tcagcttatg atgctagcca gctaacagct accaaagcct tgtcagattt
    12121 ctttgaaact gcagtaagtc ttggtggcga tgccaagcaa gtctctaact ggttgcaagg
    12181 agaagttgcc caattcttaa acgcagaagg caaaaccatt gaagaaatcg ctctgacacc
    12241 agaaaatttg gtggagatga ttgctatcat tgctgatggt acaatctctt ctaaaatggc
    12301 taagaaggtt tttgtgcatt tggctaaaaa cggtggctca gcacgtgctt atgtggaaaa
    12361 agcaggactc gttcagattt ctgatcccgc tgttttggta ccaattattc accaagtctt
    12421 tgctgataat gaagctgcag tggctgattt caaatcaggc aaacgtaatg ctgataaggc
    12481 tttcacaggc ttcttgatga aggcaaccaa aggtcaagct aatccacaag tcgctcaaca
    12541 attattggca caagaattgc aaaaattgcg agattgatag ttgctgatag caataagtta
    12601 aagtattttg tctctgaaaa aaaggcggtc ggtctttcac aggctgcctt tttgtatgtc
    12661 atgttagtga ccgcctataa aaagaccatc atctatgcta aaataagact gacaggtatt
    12721 gctttagaac cttcaggagt ttctgacggg tatttaatga accatcacgt ttgtccacat
    12781 tattattagc gattagctgt gtgataaagt ttgttaagtt atttagtgtg tgaagacttc
    12841 tagtcgttct cggtctatct atcttaatag aaggaattgt tttttctatt ttactcaggc
    12901 acggaaacac ttactaaaga agaaggtaaa ggaatcaact aaagttaaga acatttaatg
    12961 gatagcagtt cttctaaaag gattttacaa gaaaggaggt gttaacgatg atatggtctt
    13021 taattgtagg tgcctttatc ggcatgatag caggtaaaat gactgaaaaa agccagtcca
    13081 tgggttgttt tactaatatt atcacaggtc tcatagggtc agctgttggg caaaggcttt
    13141 ttggggattg gggaattcag ttggcaggta tggctatttt cccgtccatt ttgggagcag
    13201 caattgtaat tgtacttgtt tcagctattt ttggaaaaag gtattaaaaa aaggctgcat
    13261 ggaagtttcc aagcagcctt ttttgcaaca taaaatgtct tatcttttta ttggttgcat
    13321 cctcattata agatagttgt gtgaaaattt aatatcgttt tcttgacaaa tagttagatt
    13381 ttgttactgt tttgacatca ttttgtaacg tcagtttagt tgtagtgtgg gtctgttgtt
    13441 atatatattt gagcgaaaca cacaaatgta attgtttgga aatttatgtg atgatttgaa
    13501 ttgtagcaat agcattgttt ttaataaaat agacaataag tcagtggtct atttgatctc
    13561 aaatagccat caggaagtgt tttgcaagtg ttttctttaa atgctataat aattaataag
    13621 acagtttagg aggttgaaat gacaaaaaca aggttgtata ttgccagaca tggaaaaacg
    13681 atgtttaata ccattggtcg cgctcagggt tggagtgaca cacctcttac aaaaaaagga
    13741 gaagaaggta ttcgagaact tggcctaggt ttaaaagatg caactatacc atttaaggct
    13801 gcgttttcaa gtgattcagg tcgaaccatg cagacaatag aaattatttt aagagaatca
    13861 gaaaacgaat ttttgcctta tactaaagat aatcgtattc gtgagtggtg ttttgggagt
    13921 ctagaaggga cttatgattc agaattattt ctaggtgtcc ttccaagaac aaaagccttt
    13981 gaaaatcgtg ataacctacg tgatgttccc tattctgaac tggcagaaag catcgtggaa
    14041 gtagataccg ctaattgggc agaaccatgg gaagttcttc gtaagcgcat ttgggagggg
    14101 tttgaagcta ttgccttgtc cattcaaaat gctggcggcg gaaatgctct tgtggttagc
    14161 catggtatga caattggaac gtttctgtgg ttgattgatc cagatcgaga caaacagtat
    14221 attgataatg ggagcgttac agttgttgaa tttgatgatg gccaatttac gataaagact
    14281 attggagata tgagttaccg ctaccgcggt cgtgaaataa tagaagaacg gtcagatgaa
    14341 atataattcg ttaaactatt tagtgcaatt gctaattgtt attgtttttt taggagggct
    14401 ttacctcttt ataaaaccag aagagtcagt aacaccaact cagttgaata aaaaagaaat
    14461 ccaacaaaaa gatatcaaaa aaacggaccg tttacgagct ttacctaagg tatctgttga
    14521 agattgggag ttggttttag taaaccgcga tcacattaca aaagaaatga gtcctgaatt
    14581 agctgacatt aatggtattt ctgtggataa acgcattgag caagccacta gtgacttttt
    14641 agcagcagca caagctatcg actcgcaaga acatcttatc tctgggtacc gttccgtaga
    14701 ctaccaaaca gagctttacc aatcttctat taaaaaagag atggctaacg atccaacatt
    14761 gacacaagaa gctgcggagg ctttggtgca aacctattca cagccgccag gggctagtga
    14821 acatcacact ggtcttgcta ttgacatgag cactgtcgat actttaaatg ccagtgatcc
    14881 atcagtagct aaagcagttc aaaaaattgc acctgattat gggtttgtct tgcgctttcc
    14941 agaaggaaaa aagactagta caggggttga ttatgaggat tggcattacc gctatgtagg
    15001 caaggcgtct gctcgttata tggctcagca caacctaacg ttggaagagt acattgctgc
    15061 tttaaaggag aaacgatgag aaaacgacta aagtttccgt attttttaac gcttctagct
    15121 tgctttttat tgctgcttgt ttgtccctta ctgagtagcc aaaggatagc tagtgcagat
    15181 aaagaagtaa gggtgaacta cagccaaaaa caatttatta caaaaatggg taaagaagtt
    15241 aaaccattgg caaaatatta tggcattaga ccatctattt tgattgctca aattcttttg
    15301 gaaacccatg atggaaaaac attactagcg tctaagtatc ataatctttt tagcaagaaa
    15361 gcaactccag gacaagcagc cattacccta aagtccccta aacaaaccaa ccaaaacgtg
    15421 agatatgcta tttataaaga tgacgctagt gcaattagag attatttacg aatgcttcgg
    15481 cagggcaaag aagtcgataa gcgtttgtat cgtaatcttg ctacagaaaa aggctataaa
    15541 gcaccagcta aaagtttaca aaagtatttg cattatactg ataaaaccta tgctaggcga
    15601 ctaattcagg ttattgaaag taacgattta acaaactatg actgaaatta gcactcttta
    15661 taaaagagtg ctaatttttt tgagtttttt tcttgacatg tctcccataa agagtataat
    15721 agaattataa attagcagtc tactaaatag agtgctaaac gaaagggagt ggttggtttg
    15781 tgattacaca acgtcaaaat gatattttga atttaatcgt tgagctattt acacaaactc
    15841 atgaaccagt agggtctaaa gctttacaga gaacgattga ttctagtagc gccactattc
    15901 gtaatgatat ggcaaaacta gagaagttgg gattgttgga aaaagcgcac acttctagtg
    15961 gacggatgcc aagtccagct ggttttaagt attttgtaga gcactccctc aggctagata
    16021 gtattgatga gcaagatatt tatcatgtca taaaagcttt tgattttgaa gcattcaaat
    16081 tagaagacat gcttcaaaaa gctagtcaca ttttagcgga gatgacgggc tatacatccg
    16141 ttattttaga tgtggagcca gctcgtcaac gtttaacagg gtttgatgtt gttcagctta
    16201 gcaaccatga tgctctagca gtcatgactt tagatgaatc taagccagtg acagtgcagt
    16261 ttgctattcc aagaaacttt ttgacacgtg acttgatagc atttaaagct atcgtagagg
    16321 agcgcttgct ggataatagt gtcatagata ttcattataa gttgaggaca gaaattcctc
    16381 agatcgttca aaagtatttt gtgactacgg ataatgtttt acaactattt gattatgttt
    16441 tttcagagct gtttttggaa acggtatttg ttgctggcaa ggtaaattct ttaacgtatt
    16501 cagatctatc aacgtatcag tttttggata atgaacagca agtagcaatt tctttgaggc
    16561 aaagtctgaa agaaggtgag atggcttctg tccaagtggc agatagtcaa gaagccgcac
    16621 ttgcagatgt gtcagtttta actcacaaat ttttgattcc ttaccgtggg tttggactcc
    16681 tgagtttgat tggtcctatt gatatggatt atcgtaggtc tgttagtttg gtaaacatta
    16741 ttggaaaggt attggctgct aaattaggtg attattatcg atacctaaat tctaaccatt
    16801 atgaagttca ctgataatgg cagtattcaa taagttattt aaaaggaggc actcagtgtc
    16861 agaagaaatt aaaaaagacg atttacaaga agaagtggaa gccactgaaa ctgaagagac
    16921 agttgaggaa gttattgaag aaactcctga aaaatcagag ttagaacttg caaacgaacg
    16981 tgctgatgag tttgaaaata aatacttgcg agctcatgca gaaatgcaga atatacagcg
    17041 tcgctctagt gaagaacgtc aacaacttca acgctatcgt tctcaggatc tggcaaaagc
    17101 aatcttacca tcgcttgaca atctagaacg tgccttggct gttgaagggt tgacagatga
    17161 tgttaaaaaa ggtttagaaa tgactcgtga tagccttatt caagcactaa aggaagaagg
    17221 cgtagaagaa gtagaagtcg actcgtttga ccacaacttt catatggcag ttcagaccct
    17281 cccagccgat gatgaacatc cagccgatag cattgcagaa gtctttcaaa agggatataa
    17341 attgcacgag cgtctgctaa gaccagcgat ggtagttgtt tataactaag gtaaatgagt
    17401 tttcgttttt gtccgtaatg acagtaaact agatagcaag ttagaagcta tttcgcttgc
    17461 tgattaaact atagtgattg cttagaattg gaagtaaaat aattcgagtg cttactaaga
    17521 taaattgaaa taaaaagtaa taaagtatta taaaataaga ggtattaaca tgtctaaaat
    17581 tattggtatt gacttaggta caacaaactc agcagtagca gttcttgaag ggactgaatc
    17641 aaaaatcatt gctaacccag aaggcaatcg tacaactcct tcagtagtat cattcaaaaa
    17701 tggtgaaatt atcgtgggtg atgctgcaaa acgtcaagca gtgacaaacc cagaaacagt
    17761 aatctctatt aaatctaaaa tgggaacttc tgaaaaagtt tctgcaaatg gtaaagaata
    17821 tactcctcaa gaaatttcag caatgattct tcaatacctt aaaggttatg ctgaagacta
    17881 tcttggagaa aaagtagaaa aagcagttat tactgttcca gcttacttca acgatgcaca
    17941 acgtcaagca actaaagacg ctggtaaaat tgcaggtctt gaagtagaac gtatcgttaa
    18001 cgaaccaaca gcagctgcac ttgcttatgg tatggacaag actgacaagg atgaaaaaat
    18061 cttagttttt gaccttggtg gtggtacatt tgacgtatca atccttgaat taggtgatgg
    18121 tgtcttcgac gttcttgcaa cagcaggtga taacaaactt ggtggtgacg actttgacca
    18181 aaaaattatt gatttcttag tggctgaatt taagaaagaa aatggtattg acttatcaca
    18241 agataagatg gcacttcaac gcttgaaaga tgctgctgaa aaagctaaaa aagatctttc
    18301 aggtgtgaca caaacacaaa tttcattacc gttcatcact gctggttctg ctggtcctct
    18361 tcacttagag atgagcttat ctcgtgctaa atttgacgac ctcactcgtg accttgttga
    18421 acgtacgaaa actccagttc gtcaagctct ttcagatgca ggattgtcat tgtcagaaat
    18481 tgatgaagtt atccttgttg gtggatcaac tcgtatccca gcagttgtcg aagctgtaaa
    18541 agctgaaact ggtaaagaac caaataaatc tgtaaaccct gatgaagtgg ttgctatggg
    18601 tgctgctatc caaggtgggg ttatcactgg ggatgtgaaa gacgttgtcc ttcttgacgt
    18661 aacaccattg tcacttggta ttgaaacaat gggtggtgtc ttcactaaat tgatcgaccg
    18721 caatacaact atcccaacat ctaaatcaca agtcttctca acagcagcag acaaccaacc
    18781 agccgttgat atccatgttc ttcaaggtga acgcccaatg gcagcagata acaagactct
    18841 tggtcgcttc caattgactg atatcccagc tgcacctcgt ggaatcccac aaattgaagt
    18901 aacatttgat atcgataaaa acggtattgt ttctgtaaaa gctaaagacc ttggtacgca
    18961 aaaggaacaa cacatcgtta tcaaatcaaa cgacggactt tctgaagaag aaattgatcg
    19021 catgatgaaa gacgctgaag ctaatgccga agccgatgcg aaacgtaaag aagaagttga
    19081 ccttaaaaac gaagttgacc aagctatctt tgctactgaa aaaacaatca aagaaactga
    19141 aggtaaaggc tttgatacag aacgcgatgc agcgcaatca gctcttgacg agttaaaagc
    19201 tgcgcaagaa tctggcaacc ttgacgacat gaaagctaaa cttgaagcat taaatgaaaa
    19261 agcgcaagct ttggctgtta aaatgtacga gcaagctgca gcagctcaac aagcagcaca
    19321 aggtgcagaa ggtgcacaag ctaatgattc agcaaataat gatgatgttg tagatggcga
    19381 atttacagaa aagtaatgat ttagttatct agtaacatta atatccgaat tcagaggttg
    19441 caccaaacct ctgtttttgg ctaaataaaa tgtaaaaatg ctgacgtcaa aatattttaa
    19501 gaaaggaata caagttcgat tattcgaaca caggctaaag cgtgtaaaga ctcttttgcg
    19561 gatttgaaca cgcctacgac cctgcgaaaa aagagaaata cctcctagat acgagagtat
    19621 ctttgtcgga tttcctattt ttcattgagt cgttttacgg cttggtatct tacattatga
    19681 acaatacaga atattacgat cgtctggggg tttctaagga cgcttctcag gacgatatca
    19741 aaaaagctta tcgaaaaatg tctaaaaaat atcatccaga tatcaataaa gaagctggag
    19801 cggagcaaaa gtataaggat gttcaagaag cttacgaaac cttaagtgat tcacaaaagc
    19861 gtgctgctta tgatcaatac ggcgctgcag gtgctcaagg tggctttggt ggtggagcag
    19921 gcggctttgg tggttttgac ggaggtggct ttggtggttt tgaagatatc ttctcaagct
    19981 tctttggtgg cggtggaagc cgaaacccaa atgcccctcg tcaaggtgac gatttgcaat
    20041 atcgtgttaa tttatctttt gaagaggctg tatttggtgt tgaaaaagaa gttagctata
    20101 atcgcgaagc aacctgcggc acttgtttag gctcaggtgc aaaacctggt accgctccag
    20161 taacttgtcg taagtgccat ggttcaggtg taatgacaat tgacacccaa acaccacttg
    20221 gtatgatgcg tcgacaagtc acctgcgata tttgtcatgg tagtggtaag gaaattaaag
    20281 aaccctgtca aacctgtcac ggtactggtc atgaaaagca agctcataaa gtgtctgtta
    20341 agattccagc tggtgtagag actggtcagc aaatccgttt acaaggtcaa ggagaagctg
    20401 gctttaatgg cggaccttat ggtgatttat ttgttatttt gaatgtctta ccaagtaagc
    20461 aatttgaacg caatggttcc actatttact acaacttaga tatttctttt actcaagcag
    20521 ccttaggaga tactgttgaa attccaacgg ttcatggcga tgttgagatg gctatccctg
    20581 ctgggacaca aacaggtaaa accttccgcc tcaaaggcaa aggagcacca aaactccgtg
    20641 gtggaggtca aggggatcaa catgttaccg ttaatattgt gaccccaacc aaactcaatg
    20701 acgctcaaag ggaagccttg caagcatttg cagaagcgag tggtgaaaaa atgttacatc
    20761 caaagaaaaa aggttttttt gataaagtga aagatgcctt agaggatatt taaagatgtc
    20821 aaacgtataa tctcaggata attttcccga agttatacgt ttttttacgt caaaacagtt
    20881 agagaaaacg ttttctttta tagatatcgt ttttttctct tgtcaaaatg gtttatttag
    20941 tatatcataa ttaggtaatt gatttgatta tcaaatgatt ttaaagagaa ataatttctt
    21001 agtcaaataa attatcagaa aagaggattg ttatgcagtt taaacacatc atctttgatg
    21061 ttgtagatga tcttgcgaca ttgactttaa accgtccaga ggtttcaaac ggttttaata
    21121 ttccaatttg tcaagagatt ctggtagcat tagcagaagt taaacgcgat acatctgtgc
    21181 gttttttatt aattaaagcc gttggaaaag tgttttcagt tggtggtgac ctagttgaaa
    21241 tgcaagaagc tgttgcaaaa gataatgtgc agtcgctagt caagattgct gaactggtac
    21301 aagaaatttc ttttgcaatc aagcatttgc caaaaccagt catcctttgc gccgatggag
    21361 ctgtagctgg agcggctttt aatatagctt tagctgttga cttttgtatc gctagtactc
    21421 aaacaaaatt tatccaagct tttgtaaatg ttggactagc accggatgcc ggtggtttat
    21481 ttctattgac tcgtgcagta ggacttaatc gtgcaactca ccttgtgatg acaggtgaag
    21541 gaattactgc tgataaaggt ctcgattatg ggtttgtcta ccgtacggca gaatctgata
    21601 aactagacaa agtttgtctt caacttttaa agcgattgag acgtggctct tcaaattctt
    21661 atgctggaat gaaatcattg gtctggcaaa gctttttcac aggatgggaa gattatgcta
    21721 aggcagagct tgctattcaa gaggagctag ctttcaagga agattttaaa gaaggtgtca
    21781 tagcttttgg agaaaggcgc cgaccaaatt ttcaaggtaa gtagactcat gcttgcaaag
    21841 tgcttttaac tctgatataa tttgactgtc aaagttttat tgttaggagg tatttgcttg
    21901 gaatatgaca aaatcaatca ctacttggtt gatatcttca accgtatttt ggtaattgaa
    21961 gagatgagtt taaaaaccag tcaatttaat gatgtctctt taaaagagat gcacactatt
    22021 gaaattatcg gcaaatatga tcaggtgact ccaagtgata tcgccagaga attaatggta
    22081 acgttaggga cagtaaccac tagcttaaat aaattagaag caaaaggtta tatcgcaaga
    22141 acgcgatcac gctcagatag gcgagttgtt tacctgtcat taacaaaaag gggtcgactt
    22201 ttggaccgcc tccatgcaaa gttccataaa aatatggttg gacatgtcat tgctgatatg
    22261 agtgatgaag agatgcaggc attggtgcgt ggactaggga atctacatca atttttggag
    22321 gatttggttt aaatgatttt ttctaaaatc agtcaggtag cccactatgt tccacagcag
    22381 ttagtgacta ataatgattt agctaacata atggacacta gtcatgagtg gatcttttct
    22441 cgaacaggaa tagcagaacg ccatatttct agagatgaaa tgactagtga cctagctatt
    22501 caggttgctg atcagctttt aactcagtct gggttaaaag ctgatgctat tgattttatt
    22561 attgttgcaa ctatcagccc tgatgccact atgccatcaa cagctgcgaa agttcaagca
    22621 gctattgcgg ctacaagtgc ttttgctttt gatatgactg ctgcttgtag tggctttgtc
    22681 tttgccttgg ctatggcaga taagcttatt gcatcaggtg cctaccaaaa cggaatggta
    22741 ataggtgcag agactttatc taagttagta aactggcagg atcgagcgac agccgttctt
    22801 tttggagacg gagctggtgg cgtgctactg gaagccagta aagataagca tgttttagcg
    22861 gaaacattac acacagatgg tgcgagatgt cagagtttaa tttcaggaga aactagtctt
    22921 tcatctcctt actcaattgg caaaaaagcc atagcgacta ttcagatgga tggacgtgcc
    22981 atatttgatt ttgcgattag ggatgtgtca aaaagcatcc tcacactaat ggcgcaatca
    23041 gatattacaa aagatgatat agactattgt ttattgcatc aagctaatcg tcgtatactt
    23101 gataaaatag cacgcaagat tgatgttcct cgggaaaaat tccttgaaaa catgatgcgt
    23161 tatggtaata ctagtgctgc tagtatccca atcctcttgt cagaggctgt tcaaaaggga
    23221 cagattaggt tagatggtac ccaaaaaatc ttactttccg gttttggtgg aggtttgaca
    23281 tggggaagtc taattgttag aatctagtta ttcatgtgcc tagcacattt ataaaaacaa
    23341 atttatattt tttaaggaga aataaacatg gcagtatttg aaaaagtaca agaaattatt
    23401 gttgaagaac ttggtaagga gacagaagaa gtaacactag aaactacatt tgatgattta
    23461 gatgcagatt cacttgatgt tttccaagtt atttcagaaa ttgaagatgc ttttgatatt
    23521 caaattgaaa cagaagaagg attaaatact gttggtgacc ttgttgctta cgttgaggaa
    23581 aagtcaaaat aaggataaaa gtgacaagta acatcagaag tctatgaaaa gcttactgct
    23641 gttatgttca tgttcatcac taaaaaaaac aataagtatt aatcaaacca ttagggtggg
    23701 attagactct ttttaggatc tagctcgctc tttcttctta ctataatagt ttgattatca
    23761 aattattctc cttatttttt atagaaagtt attatgaaaa cacgtattac agaattactt
    23821 aatattgatt accccatttt tcaaggagga atggcttggg ttgctgatgg tgatttagca
    23881 ggtgcagttt ctaatgctgg tggtttaggc attataggtg gtggcaatgc tcccaaagaa
    23941 gtcgttaaag ctaatattga tcgtgtcaaa gctattactg atagaccttt tggggttaat
    24001 atcatgcttt tatctccttt tgctgatgat atcgttgatt tggtcattga agaaggtgtt
    24061 aaagtagtaa caacaggcgc aggaaatcca ggaaagtata tggaaagact gcaccaggcg
    24121 ggtataatcg ttgttcctgt tgtcccaagc gttgcgctag ccaaacgtat ggaaaagcta
    24181 ggggtagatg ctgttattgc tgagggtatg gaagctggag gacatattgg caagttaacg
    24241 actatgtctt tagtaagaca agttgttgaa gcggtttcga ttcctgtcat tgcggcaggt
    24301 ggtatagctg atggtcatgg tgcagcagca gcatttatgt taggagcaga ggctgttcaa
    24361 attggaactc gctttgttgt tgctaaagaa tccaatgccc accaaaattt taaagataaa
    24421 atcttagcag caaaagatat tgatacggtg atttctgcgc aggttgtggg ccaccctgtc
    24481 cgttctatta aaaataaatt gacctcagct tacgctaaag cagaaaaagc atttttaatt
    24541 ggtcaaaaaa cagctactga tattgaagaa atgggagcag gatcgcttcg acatgctgtt
    24601 attgaaggcg atgtagtcaa tggatctgtt atggctggcc aaattgcagg gcttgtgaga
    24661 aaagaagaaa gctgtgaaac gattttaaaa gatatttatt atggtgcagc tcgtgttatt
    24721 caaaatgaag ctaagcgctg gcaatctgtt tcaatagaaa agtagataaa cacatgaagg
    24781 ataatgataa tgacaaagac agccttttta tttgccggtc aaggtgccca aaaattaggg
    24841 atggcaaggg atttttatga taactttgct attgtaagaa aaacctttga tcaagctagt
    24901 caagtattgg gatacgattt gcgccgtttg attgatagtg acgagttaaa acttaatcaa
    24961 actagctata ctcagccagc tattttgaca tcatcaattg ctatttaccg tgttttgggc
    25021 ttacatcacg ttaaaccgga tatggtagct ggtctttcct taggagagta ctcagctttg
    25081 gtagcatcag gggcactctc ttttgaagat accctatcct tagtagctaa gagagggcgc
    25141 ttgatggagg aagcagcacc acaaggatct ggaaagatgg ttgccgttat gaatacagac
    25201 gtgcaagtca tcgaagaagt ctgtcaaata gctgctaaac atggagtggt tgctccagca
    25261 aactataata ctcctagtca aattgtaatt ggtggtcaga cagatgctgt gaacgtggca
    25321 gttgaacttt taaaggaaag gggagttaag cgtttaatcc ctttaaacgt gtcaggtcct
    25381 ttccacactg ctttgttaga accagctagc cgcttgttgg ctaaagagtt ggaaagatac
    25441 aacttcagtg acttcaagat tcctttggtt ggtaataccg aagctaatat tatggaaaaa
    25501 gaccgtatcc cagaactatt agcccgtcaa gtcatggagc ctgttcgttt ttatgacagt
    25561 gttgcgactt tagtagaaag tggcataaca caattcattg aggtaggacc aggtaaggtt
    25621 ttgacaggtt ttgtgaagaa aattgataaa aatttactat gtactagtgt tgaaaacatg
    25681 gtaagcttac gtttattttt agatagagag gacattcatg gaaattaaag gaaaaaatat
    25741 atttatcaca ggctcaacga gaggtatcgg tttagcaatg gctcatcaat tcgccagttt
    25801 aggagctaat attgtcttaa atggtcgctc tgctatctct gaggagcttg ttgctagttt
    25861 tactgactac ggcgttacag ttgttaccat ctctggggat gtttcagaag catcagaagc
    25921 taaacgtatg gtcaatgaag ctatcgaaag tttaggttcg attgatgttt tagttaacaa
    25981 tgctggcatt actaatgata aattgatgct taaaatgacg gaagaggatt ttgagcgcgt
    26041 cttgaaaatc aatttgaccg gtgcttttaa catgactcaa tcagtgttaa aaccaatgat
    26101 caaggcccgc caaggagcaa ttattaatgt ctctagtgtg gttggtttaa caggcaatat
    26161 tgggcaagca aactatgccg cttctaaggc aggaatgatt ggttttacca agtctgttgc
    26221 ccgtgaagtt gctgctcgta acatttgcgt gaatgctatt gctccaggat ttatcgagtc
    26281 cgacatgaca ggtgtccttc ctgaaaaaat gcaagagcaa atcttaagtc aaatcccaat
    26341 gaaacgtatt ggaaaagctc aagaagttgc tcacttagca agcttcttgg tggaacaaga
    26401 ttatattacg ggacaagtta tcgctattga tggcggtatg acaatgcaat aaggagacgt
    26461 agtgaaatga catttaaaag agtagtggta acaggttatg gtctaacatc tccaatcggg
    26521 catgatcctg aaacgttttg gaacaatctg aaagctggcc aaatcggtat tggccctatt
    26581 actaaatttg acacgactga ttatgcggtc aagaatgctg ctgaaattca ggattttcca
    26641 tttgacaaat actttgtgaa aaaagatttg aaccgtttcg atagatattc tctttatgct
    26701 ctttacgcag cgaaagaggc tattaatcat gctgatttaa atattgagat gattgattcc
    26761 gatcgatttg gcgttattgt cgcttcaggt attggtggaa ttgcggagat tgaagagcag
    26821 gtgattcgct tacatgaaaa aggaccaaaa cgtgtgaagc caatgacgtt gccaaaagcg
    26881 ctaccaaata tggctgcagg aaatgtcgcc atgtctttaa aagctcaagg agtatgtaaa
    26941 tcaattaata ctgcctgcgc ttcttcaaat gatgcgattg gggatgcctt tcgtgccatt
    27001 aaatttggga ctcaagatgt gatgatagtt ggtgggtcag aggcggccat aaccaagttt
    27061 gccattgctg gcttccaaag tttaacagct ctctcaacaa ctgaagatcc aagcagaagc
    27121 tctattccat ttgataaaga ccgaaatggt tttatcatgg gagaaggatc aggaatgctt
    27181 gttttagaaa gcctagaaca tgcgcaagaa cgcggggcga caatcttagc agaaattgtg
    27241 ggttatggaa atacctgcga tgcttaccat atgacatctc caaatcctga gggattagga
    27301 gctcgtaaag ccatccactt agccttacaa gaagctggta ttgaagccag tgctattaat
    27361 tatgtcaatg cgcacggaac ttcaacccca gctaatgaaa aaggagagag tcaagcaatt
    27421 gtagccgttc ttggtaagga tgttccggtg tcttcaacaa aatcgtttac gggtcacctt
    27481 cttggagcag caggcgctat tgaagctatt gccactatcg aggctatgcg tcataactat
    27541 gttccgatga cggctgggac acaagcttta tcagaggata tcgaagcaaa tgtcatcttt
    27601 ggagaaggaa aagaaacagc tatcaactat gccatttcaa atacttttgg atttggtggt
    27661 cataatgctg tattagcttt caaatgttgg gaggagtagg ttgaatattc aagaaatcaa
    27721 agatttgatg gctcagtttg atacctcaag cctacgtgaa tttttattca aaacaaatga
    27781 aggtgagcta atctttagta aaaacgaaca acacctcaat gcatcgacat caaatcaaga
    27841 acatgctgtg cctgtaccac aggtacaact tgttccgaat ccaactgctt cggaagcgtc
    27901 tagtccagca tcagttaagg atgttcctgt agaggagcaa ccacaagcag aatcttttgt
    27961 agcagaaggt gatattgttg aaagtccttt agtcggtgta gcctatcttg cagctagccc
    28021 tgacaagcca ccatttgtgg cagtcggaga tactgtaaaa aaaggacaaa cccttgtgat
    28081 tatcgaagcc atgaaggtga tgaacgaagt gccagcgcca tgtgatggtg tgattacaga
    28141 aattttggta tcaaatgaag atgtcattga atttggacaa ggattggtgc ggatcaaatg
    28201 atggatatta gagaaattca agcggctttg cctcatcgct acccaatgtt attggttgat
    28261 cgtgtattag aagtgtcaga cgaccatatc gttgccatta aaaatgtgac tattaatgaa
    28321 cctttcttta acgggcattt tcctcactat cctgtgatgc caggtgttct gattatggaa
    28381 gctttggctc aaacagctgg tgtcttagaa ttgtcaaaag aagaaaataa aggcaagttg
    28441 gtcttttacg ctggaatgga taaagtgaag ttcaaaaaac aagtcgtccc tggtgatcaa
    28501 cttgtgatga cagcaacctt tatcaagcgt cgcggtacca ttgctgtcgt tgaagccagg
    28561 gcagaagttg atggcaagct agcagcaagt ggaacattaa cctttgcctg tgggcaatga
    28621 gaatgattgt tctgcggagg aagaaaccct tatgtttaaa aaaatcttaa ttgctaatcg
    28681 tggtgaaatt gcagtgcgta tcattcgtgc tgcacgagaa ttagggattt ccacagttgc
    28741 agtttattcg gaagcggata aggaagctct gcacacgata ttggcggacg aggctatctg
    28801 tattggtcct gcccgttcca aggaatccta tcttaatatg aattccgtct tgtcagcagc
    28861 tattgtgaca ggagcgcaag cgattcaccc agggtttggc tttttaagtg aaaactcaaa
    28921 gtttgcgacc atgtgtgaag agatgaatat taaatttatc ggtccatccg catcggttat
    28981 ggataaaatg ggtgataaaa tcaatgctcg ttctgaaatg attaaggcag gagtgcccgt
    29041 tattccaggg tcagatggtg aagtttataa cgctcaagaa gccttagcta ttgccaataa
    29101 gattggttat cctgtcatgc taaaagcatc ggcaggtggt ggaggtaaag ggattcgtaa
    29161 agtggagaca gaagcagatt tggaggcggc tttcaatgct gccagtcaag aagctttggg
    29221 agctttcgga aatggcgcca tgtatttgga aaaagtaatt tatccagcta gacatattga
    29281 ggtgcaaatt ttaggagacg cttacggtaa catcattcac ttgggagaac gggattgctc
    29341 gttacagcgt cataatcaaa aagtcttgga agaaagtcca tccattgcta ttggcaatac
    29401 cctcagacac gaaatggggc aagcagcggt tagagcagct gaagctgtag cctatgaaaa
    29461 tgcgggaacc attgagtttt tgttggacga agacagcgag aaattctatt tcatggaaat
    29521 gaatacccgt atccaagttg agcatcctgt caccgaattt gtgacaggag tggatattgt
    29581 caaagagcaa atcaaaattg cagcaggtca gcctttggcg attaatcaag aagacatcac
    29641 tatcacggga catgctattg aatgtcgtat caatgctgaa aatactgctt ttaactttgc
    29701 accgagtcca ggaaagatta cagacttgta tatgccaagt ggtggtgtag gactacgagt
    29761 tgattcagca gtttacaatg gctatgccat tcctccttat tatgacagca tgattgctaa
    29821 aatcatcgtt catggaagca atcgatttga cgctttgatg aaaatgcaac gtgccttagt
    29881 tgaattggaa attgaaggca ttatcaccaa tacagacttc cagctcgatt tgatttcaga
    29941 taagcgagtg attgcaggag attatgatac ctctttcttg atggaaacgt tcctgccaca
    30001 ttacaataaa gattaggaga aaatatggct ttatttcgta aaaaagataa atacatccgt
    30061 attactccca ataattccct caaaggatct gtcagccata atgtcccaga agttccagat
    30121 gaactctttg cgaaatgccc agcttgtaag cacatgattt ataagaaaga tttgggattg
    30181 gcaaagattt gcccaacctg ttcttacaat tttaggattt ctgcccaaga gcggctgacc
    30241 ttaacagttg atgaaggctc tttccaggaa ttatttacaa gtattgaaac caaggatccg
    30301 ttacgttttc caggttatca ggaaaaatta caaaaagcca aggaaacaac tggtctgcat
    30361 gaagctgttt tgacagggaa agctatggtt aaaggacaac aaattgcact tgctattatg
    30421 gattctcact ttattatggc tagtatggga actgttgttg gggaaaaaat tacccgattg
    30481 tttgaattgg ctatcgaaga aaacctgccg gtggtgattt ttacggcttc aggaggtgcc
    30541 cgtatgcaag agggtatcat gagccttatg caaatggcca aggtctctgc agctgtaaaa
    30601 agacattcta atgcaggcct cttttacctc actattttga cagatccaac tactggtggg
    30661 gtaacagcta gttttgccat ggaaggggat attattttag cagaacctca atccttggtt
    30721 ggatttgctg gacgccgtgt tattgaaacc acagtacgtg agaatttgcc ggatgatttt
    30781 caaaaagcag aatttttaca agaccatggt tttgtggatg ctattgtgaa acgaacagag
    30841 ttgcgagaca agattgccca cttagtagca tttcatggag gtggccaatg acagatgtat
    30901 caagagtttt aaaagaagcg cgtgatcaag ggcgtttaac aactttggat tacgccaacc
    30961 ttattttcga tgactttatg gaactgcatg gcgatcgcca tttttcagat gatggtgcta
    31021 ttgtaggtgg cttagcttat ttggcgggac aacctgttac ggtcattggt attcaaaaag
    31081 gtaagaattt acaggataat ttggcaagga attttggcca gcccaatcca gaaggttatc
    31141 ggaaagccct acgtttaatg aaacaggcag aaaaatttgg acgacccgtt gtcaccttta
    31201 tcaatactgc aggagcttat ccaggagttg gtgcggagga acgaggacag ggtgaggcca
    31261 ttgctaaaaa tttgatggaa atgagtgatc tcaaggttcc cattatcgcc atcattattg
    31321 gtgaaggggg ctctggtggt gcattagcct tagcggttgc cgatcaggtc tggatgcttg
    31381 aaaataccat gtatgcggtc cttagcccag aaggctttgc ttctatttta tggaaagatg
    31441 gttcaagagc gacagaggct gctgaattga tgaaaattac cgcaggcgaa ttgtatcaaa
    31501 tgggcatagt agatcgtatt atcccagaac atggttattt ttcaagtgaa atcgttgaca
    31561 tcatcaaagc taacctcatc gaacaaataa ccagtttgca agctaagcca ttagaccaat
    31621 tattagatga gcgctacaaa cgctttcgta aatattaaca acatcctttt ggagtctccc
    31681 tacgtgttta tcaagctaag ggaggccttt ccaaaagggt ttttaactag tcttgtaaat
    31741 aaggaaatgg gtatactttg ataaactagt gtgaagaaaa gtgttgttaa aggggcaatg
    31801 tagtgagttt caagtgatgt ggtggctctt taaaatagta aaccccagaa atcactttaa
    31861 tgatctctgg ggtgagttta ttatttaggt gaaatgacgg tttcgccacc catgtaaggg
    31921 cggagtactt cagggatggt aacagaacca tcttcatttt ggtagttttc taaaatggct
    31981 gctacggtac gaccgacagc aagtcctgac ccattaaggg tgtgaagaag tttgacctta
    32041 ccgtcagcct catcacggta acggatttga gcacgacgtg cttggaaatc ttctgtattt
    32101 gaacatgatg aaatttcacg ataggtattt tgtgcaggaa tccagacttc aaggtcatag
    32161 gttttagcag ctgagaagcc catgtctcca gtacaaaggg aaatgacgcg atatggcaaa
    32221 ccaagttttt gaagaatatt ttcagcatta gcagtcattt tttctagttc ttggtaagat
    32281 tcttctggtt tagcaaattt aaccatttca actttgtgga attggtgtag gcggataagg
    32341 ccacgtgtgt cacgacccgc agaacccgct tcagaacgaa aagatgggct cattgcagta
    32401 aagtaaattg gcaattcttt gccgtctagg atttccccac ggtaataatt ggtcaaaggc
    32461 acttcagcag ttggaatcag gacaaagttg gtatctgcta attcaaaggt gtcctctttg
    32521 aactttggat attgtcctgt accaaacatg gaatcgtggt taaccatgta aggtgtgatg
    32581 atttcttgat aaccttcttt gatatgttca tccagcatga agttataaag agcacgctcc
    32641 aaacgagcac caagattttt atagaataag aaacgagcac cagttacctt tgccccacgt
    32701 tcccagtcta aaatatcaag atcttcgcct agatcccaat gagccttaac ttcgaagtca
    32761 aagtcacgag gcgttcccca acggcgaatt tcgacattgt cttcttcgtc agctccaaca
    32821 gggacagaat cgtgaggagt attaggtaaa acagtgatga tgtcagtaac ttgctggtcg
    32881 atagctacta gttgattgtc aatagttttg atatctgcag agactttttg catatcagca
    32941 atttgttgag tggcatcttc tttttgacgt tttgcttggg cgatggctgc agaagcaata
    33001 ttacgctctg cttttaattc ttctgattgc actaatagag cacgacgttt ttcatcaagt
    33061 tctttaagat gggtcagggt gtcttcagaa acaccgcggt tttttaattt ggcagcgaca
    33121 gtgtcaaaat cggtacggat acgtttaaga tctagcatac agaagctcct ttaagatagt
    33181 tattagtagc tgtcagtgaa acaaggctaa gtaagacagt tggatcttgt gattctctga
    33241 cgatagaaaa agcacagctt atgaagatgc tggagcgctt tggcgcggtt ccatccagct
    33301 tcacaatctg tgcacttaat ctatatttta ttgatgacac ggtagaattt catatcattt
    33361 tccctatctg ttcacagcat ccacagactc tctgaatgaa aaaaaggtat tacttatccg
    33421 ttaggactat tataacaaaa aacaagaaaa agtaaactca aatgagggaa tcttttccac
    33481 taaatagcaa tttagagcta ctattctttg ctttcttgaa atgataaatc ggcactgcgc
    33541 attatttttt taatgtcgaa aaaatttgtt aataaaggtt tgcatcaagg ttggtaatgt
    33601 aatcactttc tcattaccaa tatgttgacg agtgattttt aagatttttc cagagtctcc
    33661 agaagcaaat aaaaacttgc cttggtccgt aaaaatttca aagtggcggc taactttttt
    33721 tcttgaaaca ttagccccga tgtgattgat agctgtccaa ggaatttgaa tataatcatt
    33781 cacattttta tcgttgtaaa attcaagggc cttgtcaccg agtaaaatat gaccaacttt
    33841 gcctcccatt ccaaggtagg agacagcttt tgtcttgtat tcgacactag tgtttaaaga
    33901 ttgagccatt ttagttcctt tcattgcgta gttgtagtta ttataccata ttttaagtgg
    33961 gattttcctg acataaagca taaaaaagaa gcctgagttc atctcagact tccattggtt
    34021 gattacataa tacctgcaag gtgagcaagg ataccaaatg caaacaagcc gatgatgatt
    34081 gcgattggag aaactttttt cttcaataac cacatgcaaa ggaatgtaag gagtaatcca
    34141 gctaaacctg ggataagaga gtccaattgt ccttgaaggg tttgagcttg gactttatca
    34201 aggctcatac caccaatagc gttggcaagg atagttttca actcagcacc tttaacagcg
    34261 ccatctggga agaccacata ggcaccatct gacaattgtt ttccaggaag gtcgatagta
    34321 aagttgatag atacccaacg ttgaacaagc actgcaagga taaacattcc aaggattgaa
    34381 gcacctttag taatgtcttg gaggataccg cctgacatgt ctttagtaat ttcagaacca
    34441 gctttgtaac caaattcttg agtataccat aagaaggcca tacggataag gttccatcca
    34501 aagaagaaca gaagtggacc aacgatgtta cctgttgaag ccaaagaagc accaagtgcc
    34561 ccaaggatag gacgaaccgt aaaccagaat actgggtcac cgataccagc aagaggtccc
    34621 atcataccga ttttaacccc ttggatagcc ttgtcatcaa ttggcgtacc atttgcgcgt
    34681 tcttcttcaa gggcaagtgt tacaccaatg attggagctg caacgtatgg gtgagtgtta
    34741 aagaattcca tgtggcgctc aagagcagct gcacggtctt caggagacgt atacaatttt
    34801 ttaagagcag ggataagtgc ataagcccag cccatgtttt gcatacgttc gtagttccaa
    34861 gaaccttgta agaattgtga acgccaccaa acacgttgac ggtctgattt agttaattta
    34921 atttgttcag tcatttgttc ttaccccttt ctagtagtct tctaagatgt cgccgattgg
    34981 atcagctgaa cctgctgcat taccaccgtt accacctttt ttagaaagat taaggtagat
    35041 gaaggcaatt gctacaccga taatacccat agcgataagg gttaattggc tgatagctgc
    35101 aagagcaaaa cctagggcga agaatggcca tacttcacga gtagccatca tgttgataac
    35161 catagcataa cctacagcaa caaccatcgc accaccgatt tgcatacctt cgttcaacca
    35221 gtttggcata gcattcaaaa cagattgtac ggctgaagta ggaacagcaa caagaagagc
    35281 tgctgggaca gcgatacgca atccttgaag aagaagggca ataaagtgga agcgttcaat
    35341 acctgcgaaa ttaccttcag cggcagcttt gtcaccagcg tgtacaaggg cagttgacaa
    35401 agtacgaacg atcattgtaa ggaaaagacc agcaacagca agtgggatag ctgttgagta
    35461 agcaaaggta attccttttt gagtaaagtc gccacttttg accatgatga tagcagctgc
    35521 aacagaggca agggcagcat caggggcaac ggcagcaccg atgtttgccc aaccaagagc
    35581 aagcatttga agtgtaccac caaggatgac accagcttca aggtgtccag ttacaagtcc
    35641 aatcaaagta caggcaacaa gtggttggtg catttgaaat tggtcgagga taccttcaag
    35701 accagcgaag aaggcaatga taacgaccaa aattgcagaa ataatcgaaa tatctgacat
    35761 gttgtaatcc tttcaaagta tagtaaacga ggcctttttg ctaataagtt agctagtcat
    35821 cgttttaaaa ccttatagcg tttgaaactt atttaatgtt agctttttgg ataagctcaa
    35881 ataagttttt cttagaatcg ttaggaactt tacggacgtc gaatgtaaca cctaggtcgc
    35941 gtaatttttc aaaagtagca acgtcttctt tgtccattga taaaacgttg ttaaccattg
    36001 ttttaccagt tgagtgagcc attgaaccaa cgttcaattc gttgatttca acaccacctt
    36061 caaccgctcg aagggcatct tgaggggttt ggaaaagaat aagtgcgtgt gtgttaccaa
    36121 aacgaggatc tttagaagct tcaatcaatt tgctaattgg aacaacgtta gctttgacac
    36181 caccaggtgc agcctgtttg atcaattgtt tgcgaaggtc atcttgagca acctcatcag
    36241 aagcaacaat gatacgatca gccttagaag ctggagtcca tgctgttgca acttgaccgt
    36301 gtaaaagacg agtgtcaaca cgagcaagat tgatctttaa tttaccatct ccgatgacag
    36361 ttccagcagg aatagctcct tgaagtgcgt taacaacttt ttcagttgca gcagtttctt
    36421 caactgggtt aaggtcttca ggaagggctt tgataccgtc cttagattcc ttaatgatgt
    36481 ttgcagcaac ttgttcaaca cctgcaccag catccataag acgttcagtg taagcttgaa
    36541 taagcatagg caagttgaga ccagtgatga tcgccatttt gcggtcagga ttttcacctg
    36601 ccacacgact agcttggtta aatggagaac cactccaaag gtcagctagc acaaggattt
    36661 catcatcagc atcaaattgt tggatagcat tgttgaagtg tccatacaaa tcatctggtc
    36721 cttcgtttgg catgaaagta acaacttgaa ctttctcttg ttcaccaaaa atcatagaac
    36781 cagattgatg aataccttca gcaaatttac catggctggc aataataata ccgataccca
    36841 ttcttgttct tcctccttta aaatattctt cgttgaaatg aagaagaata atctccttcc
    36901 aaaactaaat tttggcaatt ctttttcact taaacgaaat tcaagaacgt ttctatttta
    36961 aaacgttttc actaaaaaat caagcccttt cggaaaaata gtacaaaaca tgaaaaaatt
    37021 ttcagaattc ag
//
LOCUS       DATURV010000011        33073 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DATURV010000011 DATURV010000000
VERSION     DATURV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33073
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.011"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            13..450
                     /locus_tag="VCF75_000536"
     CDS             13..450
                     /locus_tag="VCF75_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEP2546127.1"
                     /translation="MNQNISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKT
                     LIGRLVEKKALGTYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTRLLLERLYD
                     LPMTLEEIEAFQELLEVKKENAVLEVPCNCLPGQCHCQKKETY"
     gene            450..2681
                     /locus_tag="VCF75_000537"
     CDS             450..2681
                     /locus_tag="VCF75_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEP2546128.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPSFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGHP
                     NMDSLVALATSFAFAYSLYGVVEISLGRAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTYQAIQKLLSLKATDVRVWRNSQWQLLPLEEISYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGQGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGVLFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQNLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNATLMATLGIDLASVQSVCAKASGKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALQQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVITNLQSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            2695..2898
                     /locus_tag="VCF75_000538"
     CDS             2695..2898
                     /locus_tag="VCF75_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEP2546129.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGEAKVT
                     GRPLTFLLKRALKDTKFELHKWS"
     gene            complement(3102..3872)
                     /locus_tag="VCF75_000539"
     CDS             complement(3102..3872)
                     /locus_tag="VCF75_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP2546130.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQSTQLLVAHENKE"
     gene            4109..4582
                     /locus_tag="VCF75_000540"
     CDS             4109..4582
                     /locus_tag="VCF75_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2546131.1"
                     /translation="MFPNILFTEARTQPEFFDLVASHLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTMILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            4575..4880
                     /locus_tag="VCF75_000541"
     CDS             4575..4880
                     /locus_tag="VCF75_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2546132.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPIVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            4906..6357
                     /locus_tag="VCF75_000542"
     CDS             4906..6357
                     /locus_tag="VCF75_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEP2546133.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYADHNHLPVLVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIVSI
                     ITLIIFIAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            6615..7040
                     /gene="lacA"
                     /locus_tag="VCF75_000543"
     CDS             6615..7040
                     /gene="lacA"
                     /locus_tag="VCF75_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP2546134.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            7055..7570
                     /gene="lacB"
                     /locus_tag="VCF75_000544"
     CDS             7055..7570
                     /gene="lacB"
                     /locus_tag="VCF75_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP2546135.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVNGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLETHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            7580..8033
                     /locus_tag="VCF75_000545"
                     /pseudo
     CDS             7580..8033
                     /locus_tag="VCF75_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            8080..9057
                     /gene="lacD"
                     /locus_tag="VCF75_000546"
     CDS             8080..9057
                     /gene="lacD"
                     /locus_tag="VCF75_000546"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP2546136.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            9254..9871
                     /locus_tag="VCF75_000547"
     CDS             9254..9871
                     /locus_tag="VCF75_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEP2546137.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEKR"
     gene            9873..10697
                     /locus_tag="VCF75_000548"
     CDS             9873..10697
                     /locus_tag="VCF75_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2546138.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLDLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPRGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(10734..11291)
                     /locus_tag="VCF75_000549"
     CDS             complement(10734..11291)
                     /locus_tag="VCF75_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP2546139.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLKTKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            11584..12426
                     /locus_tag="VCF75_000550"
     CDS             11584..12426
                     /locus_tag="VCF75_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP2546140.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(12555..13277)
                     /locus_tag="VCF75_000551"
     CDS             complement(12555..13277)
                     /locus_tag="VCF75_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546141.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            13479..15110
                     /locus_tag="VCF75_000552"
     CDS             13479..15110
                     /locus_tag="VCF75_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEP2546142.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADGKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            15228..16376
                     /gene="nagA"
                     /locus_tag="VCF75_000553"
     CDS             15228..16376
                     /gene="nagA"
                     /locus_tag="VCF75_000553"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEP2546143.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLGLHDGVFGEWIEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKNPRLEEFDAWQKAAK
                     GLIKKIALAPERDGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            16498..17337
                     /locus_tag="VCF75_000554"
     CDS             16498..17337
                     /locus_tag="VCF75_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP2546144.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDHLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSATPNPDT
                     TSF"
     gene            17469..18002
                     /locus_tag="VCF75_000555"
     CDS             17469..18002
                     /locus_tag="VCF75_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEP2546145.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            18374..19291
                     /gene="glyQ"
                     /locus_tag="VCF75_000556"
     CDS             18374..19291
                     /gene="glyQ"
                     /locus_tag="VCF75_000556"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEP2546146.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            19669..21708
                     /locus_tag="VCF75_000557"
     CDS             19669..21708
                     /locus_tag="VCF75_000557"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEP2546147.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPAH
                     TLTVLLDDEALDLDFLDIHSGRVSRGHRFLGKETTITSADSYEDDLRSQFVIADAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGNFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYARLAGEDAAVARAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAVAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAVIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRLAILSDLVSKAKTIAAFNQLNTK"
     gene            21862..22119
                     /locus_tag="VCF75_000558"
     CDS             21862..22119
                     /locus_tag="VCF75_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEP2546148.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            22246..22638
                     /locus_tag="VCF75_000559"
     CDS             22246..22638
                     /locus_tag="VCF75_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEP2546149.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            22998..24524
                     /gene="glpK"
                     /locus_tag="VCF75_000560"
     CDS             22998..24524
                     /gene="glpK"
                     /locus_tag="VCF75_000560"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEP2546150.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            24540..26378
                     /gene="glpO"
                     /locus_tag="VCF75_000561"
     CDS             24540..26378
                     /gene="glpO"
                     /locus_tag="VCF75_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEP2546151.1"
                     /translation="MEFSKETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGHQEG"
     gene            26380..27081
                     /locus_tag="VCF75_000562"
     CDS             26380..27081
                     /locus_tag="VCF75_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP2546152.1"
                     /translation="MDIFGEFLGTVLLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAYLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            27190..28536
                     /locus_tag="VCF75_000563"
     CDS             27190..28536
                     /locus_tag="VCF75_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP2546153.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRIIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QVNIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDADLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            28526..30025
                     /locus_tag="VCF75_000564"
     CDS             28526..30025
                     /locus_tag="VCF75_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2546154.1"
                     /translation="MKIEDLMDKERRAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHKHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYFEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGVILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMDQETDFSQQLSDDILALLIFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVCLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            30151..30795
                     /gene="fsa"
                     /locus_tag="VCF75_000565"
     CDS             30151..30795
                     /gene="fsa"
                     /locus_tag="VCF75_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP2546155.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMMQHPLTTNGLKTFMEDWASFKK"
     gene            31013..32998
                     /gene="tkt"
                     /locus_tag="VCF75_000566"
     CDS             31013..32998
                     /gene="tkt"
                     /locus_tag="VCF75_000566"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEP2546156.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAAYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLDGIIIATGSEVKLAMDT
                     QEALEAEDIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
BASE COUNT         9771 a         6088 c         7380 g         9834 t
ORIGIN      
        1 ggttgtgagt tgatgaatca aaatatttca gctgccgaat gggaagtcat gcgtgtggtg
       61 tgggccagtg gtgatattaa aagtagtgat attattacta ttttaaggaa aaaatatcaa
      121 tggtcagatt ctaccattaa aaccttgatt gggcgtttgg tggaaaaaaa ggctttggga
      181 acttaccggc aaggaagagc ttatatttat caagctcttt tggatgagac tttgctacaa
      241 aaagaagcac tagcgactgt tttagacggt atttgtcagc ggcaacacac gcgtctactt
      301 cttgaacggc tttatgactt accgatgacc ttagaggaaa ttgaggcatt ccaagagcta
      361 ctagaagtca aaaaagaaaa tgcagttctt gaggtgccgt gtaattgcct gcctgggcag
      421 tgccactgtc agaaaaagga gacgtattaa tggctaaaga gatattttta gtggagggaa
      481 tgagttgtgc ctcttgtgct ctaacaattg aaaaaacggt caatcaattg cctgaagtag
      541 aacaggctgt tgtgaattta gcgaccgaga aactcacggt tacttatcaa gatgacgatt
      601 tagaaacggc caaagttatt caagcggtaa aagctgctgg ttatggtgca caagtctttg
      661 atgaacatgc aaaacacatg caaacaggtc gtcgtgacga agacctaaag gttatttggt
      721 ctcaggtctt gtggtctgcc ctattcacca ttcctatatt atacctagct atgggtcaca
      781 tggtgggctt atggcttcct tcatttctgc aaccagatcg ctatcctttc gtttacagct
      841 ttagtcagct actcttgacg attccagtct tagtattaaa tcgtcattat taccataatg
      901 ggttcaaagc tttgttcaag ggacatccta atatggactc cttggtagct ttagcaacca
      961 gctttgcttt tgcttatagt ctttatggag tggttgaaat cagtctgggc cgggcgcact
     1021 ttgtgcactc cctttatttt gaatcggtgg tagtgatttt gacactcatt ggtctaggaa
     1081 agtattttga aagtcgttct aagggccgta cttatcaagc tattcaaaag ctcctatcct
     1141 tgaaggctac tgatgtgagg gtatggagaa acagccaatg gcaattgttg ccgttagaag
     1201 agattagcta tgatgacctt gtcctggttc aaccaggtga aaaagtttct attgacggca
     1261 ttatcgtgga aggacatagc agtttggatg aatccttttt aactggggaa agtttgcctg
     1321 ttgaaaaggg agaaaaagat aatgtctttg cgggcagctt gaatggtcaa ggcgccctca
     1381 cgattaaacc agataagctt gggaatgata ccttgttagc acaaattatc caactagttg
     1441 agaatgctca agaaaataaa gcacctattg cagccattgc tgatagagtc tcaggtgtct
     1501 ttgttccggt ggttttggta ttagctctgt taacaggggt gttttggttg gtattcatgc
     1561 aggaaagtct tcgttttagt ttgacaacag ccatcgctgt tctagtcatt gcttgtcctt
     1621 gtgcgcttgg tttagcaacc ccgacagcga ttatggttgg aacagggcgt gcagctgaaa
     1681 atggtgttct ttttaaaggt ggtgacatct tagaacaagc ccatcaggta gatacggttg
     1741 tttttgataa aacggggacg attacggagg ggaaaccaag tcttcaaaac ctcctaactt
     1801 tttcgggaga tgatcacctt atcttgcaag aagctgcttc cttggaggcc tattctcaac
     1861 accccttagg ggaggctatt gtccgtgctg ctcaggaagc tggttacgat agtctaccag
     1921 tggaagagtt tgagtcttta accggcctag gagtcactgg tcagcttgaa gggcgtcgtt
     1981 tggcgattgg aaatgctact ttaatggcaa cccttggtat tgatttagct tctgttcagt
     2041 cagtttgtgc taaggcttca gggaaaggtc aaacccttgt atattatgct aaagaggggc
     2101 agctaagagc tcttttcagt attgctgacg ctgtcaaaga agatagtcaa gctaccgtcg
     2161 aagctttgca gcaactggga attcacacca tcatgctaac aggtgaccac gatgctaccg
     2221 ctaaggctat tgcctctcaa gtggggatta cagatgtgat cagtcaagtc ttacctgatc
     2281 aaaaagcagg cgtgataaca aacctccaat ctcaagggag aaaagttgca atggtaggtg
     2341 atggtattaa tgatgctccg gcattggctg tagcagacat tgggatagct atggggtcag
     2401 ggacagatat tgccattgaa tcggcggatg tgattctgat gaaacctgat atgctggatt
     2461 tggtgaaagc tatgtcattg agtcgagcga ctatgaggat tgtcaaggaa aatttatttt
     2521 gggcattcat atacaatgtc ctcatgattc cagtggccat gggcttactt catctttttg
     2581 gaggtccact attgaacccg atgttagcag gttttgccat gagttttagt tcagtatcag
     2641 ttgttttgaa tgccctgcga ttaaaaggaa aaaccatttg aaggagaaaa cgaaatggaa
     2701 aaacactatc aggttacagg aatgacgtgt gacggttgtg caagaacggt tactgaaaaa
     2761 ttatcagctg tccccggtgt gcaatcggtc caggtaaatt tggaaaaagg agaggctaag
     2821 gtgacaggac gtcctttaac tttcctactc aagcgtgctt tgaaggatac caaatttgaa
     2881 ttacataaat ggtcttaggt tattttggta gccttacttc atctcaaaag aggttccagt
     2941 tttttaatct gggcttcttt ttttgtggtc actcataaag actctgacgt tagcaggtgt
     3001 gttccgagca aaacaaaaac acctctctgg gggcaaatca cttttgccag aaggtgttta
     3061 aggacgatgc gctgacaggc tgtccattct tgatgaaacg gttactcctt gttttcgtgg
     3121 gctactaata attgtgtgga ctgactataa cgttcgatga cgtcctcagt tatttgcgaa
     3181 tctgtaacaa cagcatcaat gttgtttaaa tggtaaaaaa tgaagaagtc gtaggcattg
     3241 aatttttggt tatctaccaa gaggtatttt tcaatagaat tattaaaagc tagcttttga
     3301 atctctcctt cagtttcgct ataagttgca atatcgtttt tataaatgcc attacagcta
     3361 ataaaagcct tggcaaattt taaggatgag atattttgac tagcaaggga ccctacaaaa
     3421 gctcctgtaa tagatcggta ttcaccaccg ataagaatca ggtcaatggt ttcgctgtcc
     3481 tgaaggatgt taaagacagg gaggctattg gtcacaatgc gaatctgacg attttttaat
     3541 tgctcagcga aacattctaa agttgttcct ggaccaataa agattgtttc accatcattg
     3601 actagttgtg tcgcatatga agctagttcc catttttcct ttgtttgaac ggtttgtttt
     3661 tcaatatttg accgctcttt tttattaggc atggtaatac tttgtgcacc tccatggatg
     3721 cggatcaatt tgccggcttt ttccagttca tctaagtctc tacgaactgt catatcagaa
     3781 acgtttaaag tctgaataat atcattcaca gtaacaatac cttgctcatt gactatttcg
     3841 gtaattttta gaagtctttc tctttttaac atctaagggg ctcctttcta tagtttattt
     3901 acttatgttt atcatagcat atagaccttc atatttcaat aaaacaaaat aaacaaaaca
     3961 aaaacaaaca tatagaacaa aaaatattgc gtaaaaatgt tgaattatgt tgaaggttat
     4021 actataatga ttttgtaaac ggttaccaat ttgttgatga tgagatgaca gttgttaaaa
     4081 attgatactt atagaaaggc ggtagggagt gtttcctaat attctattta ctgaagcaag
     4141 gacgcagcca gagttttttg atcttgttgc tagccacttg gaaaaggttg ggtatgtgac
     4201 gcaagagtac catcaggctt tagtggagcg tgaggcggta tttccgacag gactcaaagt
     4261 ggacctcaaa gatgggtcag atattttgta tgcggctatt ccgcatacag aaaccaagta
     4321 ttgcttggtt gatcaagtgg tctatgttag aaatagtcaa gcccttacct ttaagcacat
     4381 gattaatcca gaggaagact gtttggtaac ggatttcttc tttattatca atagtcaaaa
     4441 cgagggtcaa acgatgattc tatccaatct cattaccttc tttattacga aaggcaatct
     4501 tagctatcta gccagtctaa aagatgataa acaagctatt tcaaactatt taatagaaaa
     4561 aggagtgttt cagcatgatt aaaattctag cagcctgcgg tgcaggggtt aactcaagcc
     4621 atcaaattaa agatgcgatt gaaactcaga tgagtgaccg tggttacgat gttcactgtg
     4681 atgcggtgat ggtcaaagac atcacagaag aattggtgtc tcgttatgat atttttacgc
     4741 cgattgcaaa aactgacttg ggatttgaga tgccaattcc catagtagaa gcaggaccga
     4801 ttctctatcg tatcccaatc atgagtgagc ctgtttttgc tgaattagag cgcgtgatta
     4861 aagaaaatca tttgcagtga gttaataaaa acataaggag acaagatgat gcaacctttt
     4921 ttagacatta ttaacaagat tctgggattt cctattcagt tgggatcagg tgtggtcatg
     4981 ctgattgtca tgacaggttt agccatgatt ttcggggtta aattcacaaa ggcactggaa
     5041 ggcgggatta agttagctat cgctcttaca ggtattggag ccattattgg gattttgacg
     5101 ggggcttttt cagagtcttt acaggccttt gttaaaaata cagggatcag tttaaacatt
     5161 attgatgtcg gttgggcacc gcttgcaaca attacctggg gttcccctta taccctttat
     5221 ttcttgctgg tgatgctggt ggttaatatt gtcatgattg tcatgaaaaa aacggacacc
     5281 cttgatgtcg atatttttga tatttggcat ttgtctatca caggtctatt gattatgtgg
     5341 tatgctgatc acaaccattt gccagtcctt gtgtctcttt tgattgctac tgtggcagtt
     5401 attcttgttg gggtattgaa aatcattaac tctgatttga tgaaaccgac ctttgatgat
     5461 cttcttggga cagggtctca atcaccaatg acttcaactc acatgaacta catgatgaat
     5521 ccaatcatta tggtcttaga taagattttt gacaaggtct ttccggggct ggataaatat
     5581 gactttgatg ctgctaaatt gaacaaaaaa attggcttct ggggctctaa gttctttatc
     5641 ggaatggctc taggttttgt gattgggatt atgggagatc ctcatttcac tgttgaatct
     5701 atcaaaaatt ggtttggtct aggatttaca gctggtgcct gcttggaact tttctctttg
     5761 attggctctt ggtttatcgc agctgttgag cctttgtcac aagggattac caattttgcc
     5821 aatgctagaa tgcatgggcg tcgtttcaat atcggtttgg actggccttt cattgctggt
     5881 cgcgctgaaa tctgggcctg tgccaatatt cttgctccaa tcatgttgat tgaagccgtt
     5941 ctcctatcaa aagttggcaa tgggatttta ccattggcgg gaattattgc tatggggatg
     6001 acaccagctc ttcttgtggt cacacgtggt cgtttgattc gcatgattat ttttggttcc
     6061 ctcttattgc cattattcct cttgtcagga actatgattg ctccatttgc gacagagttg
     6121 gctaaaaaag taggggcttt tccagcaggt acaagcgctg gttccttgat tacgcattca
     6181 acacttgaag gaccaatgga aaaaatcttt ggttatgtga ttggtcaagc gacaacaggt
     6241 cagattgtct caatcatcac gctaatcatc tttattgcca tttacctagg tctctttgct
     6301 tggtatgcta accaaatgaa ggcacgtaac gctgagtatg cgaaaacaat gaaataaaat
     6361 caggtcaagt gactgactaa aaaattaggg agaatagcag ataaccgctt aaggtattcc
     6421 tgccttatcc cctaacgtga aaagagtatt tctcatgtaa ggcaaggcgc tctgtgggaa
     6481 cccacaagtg ctttgtcttg aaagaaataa tggaagacaa ctaagactgc tggcaatcat
     6541 cttgatctaa ggacaggtaa tcatgtcccc cttctctaga ttggcaagcg ttttgggtta
     6601 gaaaaggaaa agctatggct attattttag gtgcagatgc acacggaaat gcattaaaag
     6661 aattgatcaa aagttttctt caagaagaag gctatgacat tattgacgtg actgatatta
     6721 acagtgattt tattgataac acattagcgg tggctaaagc cgttaatgaa gcagaaggtc
     6781 gtttaggcat tatggtggat gcttacggag caggtccctt tatggtagcc acaaaactaa
     6841 aaggaatggt tgctgctgaa gtgtcagatg aacgctctgc ttacatgacg cgcggtcaca
     6901 acaatgcccg catgatcaca ataggggcag agattgtagg tccagaatta gcgaagaata
     6961 tcgtcaaagg ttttgtgact ggtccctacg atggtggtcg tcatcaaatt cgtgttgaca
     7021 tgctcaataa aatggcctaa aggaggataa agtgatgaaa attgcgattg gttgtgacca
     7081 tattgtcacc aacgaaaaaa tggctgtttc tgattttttg aaatctaaag gctatgacgt
     7141 tattgactgt ggcacttatg accatacaag aacccattac cctatttttg gaaaaaaagt
     7201 cggcgaagct gtggttaatg gtcaagcaga cttgggtgtt tgtatttgtg gaactggagt
     7261 tggcattaat aatgccgtga acaaggtacc aggtattcgt tcagcccttg ttagagatat
     7321 gacaacagcg ctttatgcca aggaagaatt gaatgccaat gtgattggct ttggtggtaa
     7381 aatcactggt gaacttctta tgtgtgatat tatagatgct tttattaagg cagaatacaa
     7441 agaaactgaa gagaataaga agctaattgc taaaatagcc cacttggaaa ctcatcatgc
     7501 taaccaagaa gatcctgatt tctttacaga atttttagaa aaatgggacc gtggtgagta
     7561 tcacgattag gaagaagaga tgagtttaac agttacctta aatccttctg tcgctatttc
     7621 ctatccttta gattgtctgg ctatggacac ggttaatcgt gttgacagaa cggcaaaaac
     7681 agatggtgat aaggagctaa atgtgaccag aatgttagca aaagctggtc agttatggca
     7741 acaggattta gtggtggtaa actgggtgac tttatcattc atcagttaca agaacagggc
     7801 atttctaacc aatttttcaa aatcaaaggg gaaacgagaa attgtattgc tgttttacat
     7861 gaaggcatgc agacaaaaat tttggaagct ggtccctaca gtgatgtgga cgaagccgaa
     7921 gtctctttga gccacatgag tactattgct aagccgtttg atgtcttaac ttctcaggca
     7981 gtctgcctaa gagactagct actcactatt atcaagattt gatgactatg gcaactttta
     8041 aacaattata tcagcaactt caagtaagag aggtatcaca tgacaatcac agcaaataaa
     8101 agacattact tagaaaaagt gagccatcaa ggaatcatat cagctttagc ttttgatcaa
     8161 cgaggtgcac tgaagcaaat gatggcagca catcaagaag gagaagcgac agttacacag
     8221 atagaaacat tgaaagttct cgtttctgag gaattgacgc cttatgcttc atctatttta
     8281 ttagatccag aatatgggtt gctggctact aaggtaagag ctaatcagac aggcttactg
     8341 ttagcttacg aaaaaacagg ttatgatgct acaaccacta gtcgtttacc cgattgctta
     8401 gtggaatggt cggtcaaacg gttgaaagca gcaggtgcag atgctattaa atttttgctc
     8461 tattatgatg ttgatggaga tgaacaaatc aatttacaaa aacaagctta tattgaacgc
     8521 attggttcag aatgtacagc tgaagacatt cctttttttc ttgaactttt gagttatgat
     8581 gagagaatat cagataacaa cagtgcggca tacgcaaagc ttaagccaca taaggttaac
     8641 ggtgctatgt cagtattttc ggataagcgt tttggcgttg atgtgctaaa agtagaggtt
     8701 cctgttaata tggcttatgt agaaggtttt actgaagggg aagtccatta cagtcaagct
     8761 gaggctatta aagcttttca agaccaagaa gctgccagcc acctccctta tatctatttg
     8821 agtgcaggcg tatctgccaa actttttcaa gagacattat actttgcagc agcagcaggt
     8881 gctcaattta gcggtgtttt gtgtgggcgt gccacgtggg caggatccgt tccagtttat
     8941 attactaaag gagaggacga agcaagaaag tggctatgca ctgaaggctt ccaaaatatt
     9001 gacgagttaa acagggtact cgaagagaca gctagccctt ggacggaaaa aatctaaaac
     9061 aaccttatcc tatcaagcag ctctcattga tgtgataaga taagtatggg gataattgtt
     9121 tgaatctttt gtagcaagga tgaaaatggg ttttatcctt gcttttttat ccttacattt
     9181 tatctttttc tacttatttt ttaaaggaaa acgtggtaaa ctaaaagaag actaaaagac
     9241 aatagggtag cttatgacaa ttgatatgtg ggaaatttta aatatcatcg ggaccattgc
     9301 ctttgctctt tcaggtgcta ttgttgccat ggaagaagaa tttgatattt tagggatttt
     9361 tattttaggt ttcgtgacag cctttggagg cggagccatt cgaaacaccc taattggttt
     9421 acctatagag gccttatggg ggcaaaagcc agaattcact tgcgctttct ttgctatggt
     9481 tttgattatg ctttttccta agctaatagc tagaggttgg gtacgggcag ctgttttgac
     9541 tgatgccatt ggtctggcag cttttagtgt ccaaggagcc ttgcatgctg tccgtcttaa
     9601 ccagccttta agtgctgtca ttgtagcagc tgtgttgact ggtgcaggcg gtggtgttgt
     9661 cagagatatt ctggcagggc gaaaacctag tgttttgcgt agtgaaatct atgctgggtg
     9721 gtctattctt gcagctattg ttttgcattt taaactagca gattcaacga ttgaatgtta
     9781 tgccttggtt gttttactca cgactttacg catgattggc aaccgtaaaa aatggaacct
     9841 ccctaaaatc aaatggaaag agaagagata ggatgattca actaattgca attgatttgg
     9901 acggcacctt attaaaccag gacaaacaga ttccaaaaga gaatatcaca gccatccaag
     9961 aagcagctca gtcaggctta aaaattgtct tatgcacagg ccggcctcag tcagggacaa
    10021 gaccctattt tgaccaatta gatttgacac aagaggaatt cctcattatc aataatggct
    10081 gtagtactta tagtagtcct gactggcaat tacgccattc aaaaatgttg aaggtttcag
    10141 atattgaatt gctggaagag ctaagccaaa gctttccaga tatttacctg actttaactg
    10201 aggaaaacga ttatttggtg cttgaagaag aagtgccaga ccttgtgcag gaagatggag
    10261 atttggtatt taccattgtc aagcctgtct ccttagcaga gctgtcggac actcctcgcc
    10321 tgatttttca agccatgtac ttaggagaaa aagctgcgct agatgccttt gaacgtgcgg
    10381 tgagaaatca gcttagtcag agtttccatg ttgttcgtag ccaagataat attttagaaa
    10441 ttctccctcg gggagtcagc aaggcatcgg ctctcaaaga attagtagaa gatttgggtt
    10501 taactgctga ccaagtgatg gctattgggg atgctcccaa tgacattgag atgttaactt
    10561 atgctggttt aggagttgct atggaaaatg cttcagcagc tatcaagcca ttagctgaca
    10621 aagtcacttt gactaatgat atggcgggtg tggcccaggc tatccgtcag tttgcacttg
    10681 cagctcaaaa agactaacta ttatataaaa aaaggttccc aaaaggaacc tttttacgtt
    10741 aatagaggca agcccatagc agccatttct tcggcaggaa cgatcatgtc atctgaaatc
    10801 cagtttgtca agacagaaac aatagcattg ctccaaaaaa ggcgcttata gtgcgattgt
    10861 tttttgtctt ttaatttttg ataagctttc agacgtttag tgactaatcg tgttagaaga
    10921 tggtgcaggt gatgtttgaa aatcatttta agaagctgag cttctttttt agcttcagta
    10981 aaaagataga gccaggcttg ataagcctga gttttaatat caaattgttt tagtccacga
    11041 aaaatacgac ggataacccc tactaacagt tgttctataa tggcttcttt agaggtataa
    11101 ttacgataaa aagcgttacg agatactcct gctttcgtca ccaattctga aatcgtaata
    11161 tcacctaaag gttttgtttt tagaagattt agtaaggcga tttccataga ttcacgtgct
    11221 atgcgtcgtg tttctttatt aaattgtgtt aagttttgaa gtgatctttt tgagagtgct
    11281 ttatcagtca tcacaaatac ctttcaagtg ttacatctga tgagttatca gtagcaaaaa
    11341 ccgacttaat aatgatataa ttttatggga aaagtcttag tttgtcaact aaagttagtt
    11401 atcaggagcc tcattacctc cctgtatagc tctattggat gactttacta actattgaaa
    11461 aggctagtaa taaaacatag gatttaatga ctagttatta ccattagcct agacatctta
    11521 gcgaaggctt aataatctgg tctttgttac cgaacacaaa ttagagaaaa agaaggataa
    11581 aaaatgactt ggaaaattgt aacagattca ggatgtgatc ttagaagttt gactagacaa
    11641 tccaaagagt tgcgctttga acgtgtgcct ttgaccctac agattggcac agaaattttt
    11701 agagatgatg atggattaga tatcgataat atgatgacaa ctatgtacca gtcgtctaaa
    11761 gcaacgacat ccagctgtcc aagcccagaa gcctttttgc aggcctatag aggtgcagat
    11821 aatgttatcg tgatgacaat tacagggacc ttatctggca gtcataatag tgcacgactg
    11881 gctaaaaatg aacttcttga agaaaatcct aatgtcaata ttcacctaat cgattcttta
    11941 tcagcaggtg gcgagatgga tttgctcgta ctggagcttg agcggttgat caatcttgga
    12001 ttatcctttg aagaagttgt caaacagatc acagcttatc agcaaaaaac acgtttgatt
    12061 tttgtgcttg ccaaggttga taatttggtt aaaaatggtc gactgagtaa attagtaggt
    12121 aaagtaattg gcttactcaa tattagaatg gttgggaaag ctagcaataa aggaacgctt
    12181 gaacttcttc aaaaagcgcg tggtcaaaaa aaagcagttt ctgctttgat agaagaaatt
    12241 caaaaagagg gttatgtagg cggaaaagtt tacattgctc atgctcaaaa tccaaaaatt
    12301 tgtgagcaga tcagtgaaaa aatcaaatct ttatatcctg atgctgtgat tcaaactgga
    12361 aggacctcag gtttgtgtag cttttatgct gaagacggag gacttttaat gggctacgaa
    12421 atttaatgct atctaacaaa acaaaaagct agactctttt gatttgagct agctttttta
    12481 agatgtcagg aagactaatc actaagatag ccgtctaaaa cgtttattga ttgattttac
    12541 gaaaaacttt tgtattactc ttttgatgat tgcttgtctc ctgagtcgga aggttctgaa
    12601 gaggtctgag gcggaattgg atcaggatga ctaggttgtg gcgtaacatc tgaagctgaa
    12661 gattctgagc ttgggtctaa agtaggtgac tgggaattat cataagtatc tgagacgtct
    12721 cctgggaaag gtgtccagta agagttatct gttatttctc cactttgtct tggaaattca
    12781 tcaataatgg gtttcgctgg taaaatagcg tctaaccgag attgtaaagc ggctttttta
    12841 cctttgttac ttaagtcgtt aactgctttt tgggcgatat ctttaagatt ttgattgaga
    12901 gtctcctcag cagctttaac tgcttgagta gctgcatctt cttgctcttg ctttgctttg
    12961 gcaacgttta gctgttcttg tagcgttttt ttcttagaag attctgctaa tttatcaaca
    13021 gctttctggg cttcttggat agcagctgca gtggctaaac gatcagcgtc attaactgct
    13081 ttaactgctt tttttagtaa actagcagca cttttagaat gtttagctac cttatcttgc
    13141 gtttgagggt gggatccaaa aataaggcag gagccaccta ttagagtgat agttagaaca
    13201 ctcaacatcc aataaagcgc ctctttttgt gatttagtca tggaatctcc tttatgatat
    13261 tgtttcatcg tagccatatt atacctctaa ttaaacgaaa tgaaaagatt tgtgcaaaaa
    13321 caaatgatgt aaaatatcgg aaaagcctaa caccttatca aaccaataat atgcaaatgt
    13381 tgacatcggt atacacagaa acgcttaaaa aaaccttgtg ataaagaccg taatacatta
    13441 aaatagaata gactaaaaaa atgagcgagg acaatattgt gtcagtaaat tggcaagaca
    13501 ttgcatttca tttctttgga ggcttaggtc tctttctttt cagtatcaag tatatgggag
    13561 atggtctgca acaagcagca ggagataaac taaggtatta cattgataaa tataccagta
    13621 atcccttttt tggtatctta gtcggaattg ccatgtctgc cttaattcag tccagttcag
    13681 gcgtcactgt gattacggtt ggtttggttt ctgcgggttt gctaaatttg cgacaagcca
    13741 ttggaattgt tatgggggct aatattggaa cgactatcac ttcgttttta attggtttta
    13801 aactaggaga ttacgccttg cccatgattt ttattggtgc agcctgtctt ttctttacct
    13861 ctaataaaaa gctcaacaat tttgggcgta tcatttttgg tgtcggagga attttcttct
    13921 cgctgaacct aatgggagat gccatggatc ctttgaaatc tgtgtcagcc tttcaaaatt
    13981 atttagcgac attaggtgat aaacccttcc aaggtgtctt tatcggaacg gctttgacca
    14041 tgttgattca gtcatcggca gccattatcg ggattttaca gggtctgttt tcaggcggtc
    14101 ttttgacctt acagggggct attcctattt tgttaggctc aaatatagga acctgcatta
    14161 cagctgtctt agcagctata ggttcaaata ttgctgctaa acgtgtggca gcagctcatg
    14221 tgttatttaa tttgatcggt acaattattt ttatgattat cctagtccca ttcacatcat
    14281 tgatgttatg gctacaatca aagttgagtt taacgcctga gatgactatc gctttttctc
    14341 acggttcttt taatatcact aataccatct tattaattcc tttcattagt ctactagcta
    14401 tgattgtgac tagactcatt ccaggagaag atgaagtggt caaatacgaa gccctttatt
    14461 tagatcgctt acttattacc caagcccctt ctatcgcact aggaaatgcc cacaaggaat
    14521 tggttcattt agcctcttat gcgattcaag cttttgaagc ttcttacagt tatattatga
    14581 cagctgacgg taaatttggg gaaaaggtca agcgttatga aagagcagtt gataccatcg
    14641 atgaggagtt aaccacttat ttggttgata tttctaacga agccctcagc ccaagtgaaa
    14701 atgaagtctt ggctggtatt cttgactctt cacgtgattt ggaaagaatt ggagatcatt
    14761 ctgagtcttt aggcattcta attgaaggca tcatatctaa acaaattggt ttttcaatat
    14821 ctgctcgtca agaattgaca gagatgtatc aattgacgca ttgtttaaca ctggatgcca
    14881 ttagagctat cgttgatagt gatacagact tggcgcaaac tattgtgact cgccataaag
    14941 aaattgaaga aaaagagcga cgattacgta aaacccacat taagcgcttg aattgtggag
    15001 aatgtacggc acaagcaggt attaatttta ttgatattat ttcccactat acccgcatca
    15061 cagatcacgc cttgaattta gcagagaaag tactttcaca ccagctttaa aaaaagagaa
    15121 aacgtgaatg atcatccacg ttttcttttt tgaatccaaa aagtggtata taccatttac
    15181 aaagtaaaga ataaggcgta taataaggaa aaaagggagg aaaaaagatg acatgctatc
    15241 ttaaagcaga ttgtttttat tacccaacag aggtgaaacc ggctggctac ctaggtcttc
    15301 atgatggtgt ttttggggag tggatagaaa ttgttccggc agatgcgcaa atcattgact
    15361 acaccggtta tcaaattgca ccaggtcttg tggatacgca tatccatggc tatgcgggag
    15421 ctgatgtcat ggataattct gcccaaggga ttcatcaaat gagtgaagga ctattagcaa
    15481 caggagtaac gtctttttta ccgacgacct taacttctac ctttgagcag ttagaaaaag
    15541 tatcagggac tattgcttca gttgctgatc aggtaaaagg tgctaaaata caaggtattt
    15601 actttgaagg tccttatttt accgaagaat ataagggtgc tcaaaaccca agttatatga
    15661 aaaacccacg cttggaggaa tttgatgctt ggcaaaaagc cgctaaaggg ctcatcaaaa
    15721 aaattgccct tgctcctgag cgtgatggtg tcaaagaatt tgtgtccgca gtgacaaaac
    15781 aaggcgtaac agtggcctta gggcattcaa acggtactta tcaagaggct aaagaagctg
    15841 ttcaagctgg tgctagtgtt tgggttcatg cttataatgg catgcgagga ttgacccacc
    15901 gtgaaccagg gatggtggga gcggtttata atcttccaaa tacttacgca gagttgattt
    15961 gtgatggcca ccatgtctca ccaattgctt gtgatatttt gatgcagcaa aaagggcatg
    16021 atcatgtagc catgattacg gactgtatgc gagcaggtgg ttcaccagat ggtgattacc
    16081 tcttggggga attctctgtg gttgtggcaa atggcactgc tcgtcttaaa gagagtggaa
    16141 acttggcagg atctattttg aaattaaaag acggccttag aaatgtagtg gcctggggaa
    16201 tcgcaactcc ggcagaagcc attcatatgg caacctatgt gccagctgtt tctgtcggta
    16261 ttgacgacgt atgtggtcaa atcaaagctg ggcatgcggc tgactttatt gttcttgata
    16321 aggatttaac acttgttgca acctatttaa atgggcaaaa agcttttgat gcctaagaga
    16381 tacaaaagga ttggggtagc tatgtctggc aggccaattc tttttgtatt ttctttcttt
    16441 atgatagaat agattacagt aatcgcttac agagattgat aaaaggaggt ttttgtgatg
    16501 gtaacaacag taaaaatgac aagtggctat gagattcccg ttttaggatt tggaacttac
    16561 caagcagccg atggtgagga agcttaccaa tctaccttag cagccatcaa ggctggctat
    16621 cgtcatattg atactgcagc tatttataaa aatgaagaaa gtgtgggtcg tgccatcaag
    16681 gattctgggg ttctacgaga agacttattt attacaacta aactctggaa tgacgcccat
    16741 agttatgaag gggcaaaaga cgctttagct gcttctcttg accatcttgg tctggattat
    16801 gtggatctct atcttatcca ttggccaaac ccaaaagctc tgcgtaacac ttggaaagag
    16861 gcaaatgctc aagcatggca atatatggaa gaggcggttg aagcaggact cattaaaagc
    16921 attggcgtca gcaattttat ggtgcatcat ctagaagctc ttcaagagac agctaaaata
    16981 actccagcaa ttaatcagat tcgattagcc ccaggttgct atcaaaagga agtggttgat
    17041 tattgtaagg caaatgagat tttattggaa gcttggagtc ctctaggaca aggggagatt
    17101 tttgataatg agactatgca gcagttagct aataaatatg acaaaacagt ggcacaagtt
    17161 gccttagctt ggtcgctggc agaaggtttt attcccttgc caaaatcggt gcatgatgag
    17221 cgcatcaagg aaaatatggc tatttttgat gtgagcttga cacaagaaga taaaaaaaca
    17281 attcgctatc tctcaggaat gtcagcgact cctaaccctg atactacaag tttttaggaa
    17341 agtacaaaga tttagccata attcgctaaa tcttttttga aaacggtttg acaaaggttt
    17401 atcatttagc tataatcttt attagtgttt tgtgaaagaa actattattg aaaataagga
    17461 gccgtattgt gaccatcttt ttagtgatgt tagccatgtt tactattcgt ttagtatttt
    17521 tgaagaaatc gataacaaat gaaaaagcca tattagccca aggaggacag gaatttggtg
    17581 ctcagaacac gaaatttttg acgttacttc acattatgat ttatgtgttt gcggtgattg
    17641 aagcgctgct taaacaaatt aagtttgatg gcatcagttt tttaggtctt ttgttgatgt
    17701 tattatcagt agctgtttta tatgaagtga cacgtatctt aggtgacatt tggactgtaa
    17761 aattgatgtt agcaaaagac cacaaatatg tggatcactg gttgttcaaa acgattaaac
    17821 atcctaacta ctttttaaat atcgcccctg agctggtggg catagcctta ctttgccatg
    17881 ctaaaattac ggctatgctt cttttcccat gctacattgt agtaatatat ttacgtattc
    17941 gagaagaaaa caagctatta gcggaagtga ttattccaaa tggtactcga acaaaacctt
    18001 aagctcatga tggacaaact aatggcttta tgctaaacta gactctagga aaaaggatgc
    18061 aagtatctta tctaaagcga gttcggggta gttagagccg aatggtagga ctgcagattg
    18121 gcgcttccgt ttgggcagtg tgattaagta tatttgtcaa tattgcccaa aaagatacta
    18181 tataaatgaa gtaataaatt agggtggaac cgcgttttga cgcccctagg tcttatgatt
    18241 taggagcatc aaaatgcggt tttttggtgc ctagacctgt aatacatgct agtaacatcg
    18301 tcaggtattt tactgatgac aaaaaaacga ttgtcaatga tataccattt tactactatc
    18361 aaaggagtac gaaatgtcta aaaaacttac ctttcaagaa atcatcttga ccttacaaca
    18421 atattggaat gatcagggtt gtatgctcat gcaagcctat gacaatgaaa aaggagctgg
    18481 gaccatgagt ccttacacgt tcttacgtgc tataggtcct gagccatgga atgcggctta
    18541 tgtagagcca tcacgtcgtc ctgcagatgg ccgttacgga gaaaacccaa atcgtcttta
    18601 ccaacaccac caatttcaag tggtcatgaa accatcacca tcaaatattc aagaacttta
    18661 ccttgcttct ttggaaaaat tgggcatcaa tcctttagaa catgatattc gttttgttga
    18721 agataactgg gaaaacccat caacaggatc agcagggctt ggttgggaag tttggcttga
    18781 tggaatggaa atcacacaat ttacgtattt ccaacaagtt ggtggccttg cgacatctcc
    18841 agtgacagca gaagtaactt atggtcttga gcgcttagct tcctatattc aagaagtgga
    18901 ttcggtctat gatattgagt gggcaccagg tgttaaatat ggtgaaatct tcttacaacc
    18961 tgagtatgag cattcaaaat attccttcga aatatcagac caagacatgc tgcttgaaaa
    19021 ctttgaaaag tttgaaaaag aagcctcgcg tgccctagaa gaaggtttgg ttcacccagc
    19081 ttatgattac gtgctcaaat gttctcatac gtttaacctg ctggatgccc gtggcgctgt
    19141 ttccgtcaca gaacgtgcag gctacattgc tcgtatccgt aatttggcac gtgtcgttgc
    19201 taagaccttt gttgcagaac gcaagaagct tggatttcca ctgttggatg aagcaacacg
    19261 cgcaatcttg ctagctgaag atgacgaata agtcgattta aagatgatct agttgcgagc
    19321 tttgtaatct taagagcgag caagaagtgg gcgaccgaag gtcgccgaat caaggttact
    19381 cccgctgtag tgacaattcg gtaaggttac ttatcgaacg agggagttgg cgagacttta
    19441 ggctggctaa ctaggaatgg aacacttttg ttttctgaaa ttatccactg gataatttca
    19501 ctttgcgtcc cccctaccta agggaacaat caaaaagata ctttttagag aaaggaactg
    19561 aacacgggct acggactatg tcaaaaagac caagacttcc taggtgcaag cacctgcgtc
    19621 gctttgctat tttgatggtg tccgcttaac gcctttggta tcttaattat gagtaaaaat
    19681 ctactaatcg aacttggtct ggaagaactt ccagcctacg tggtcacacc aagtgaaaaa
    19741 caactgggag agcgcttggc gactttctta acagaaaatc gtctctcttt tgaagatata
    19801 caaacctttt caactccacg ccgtttggca gtgcgtgtgt ctggattagc tgatcagcaa
    19861 acggatttga ctgaggactt taaagggcct gctaagaaaa ttgcccttga tgctgatggg
    19921 aatttctcaa aagctgctca aggatttgtt cgtggcaaag gcttgaccac agatgctatc
    19981 gaattccgcg aagtcaaagg agaagaatac gtttatgtga ccaaacacga agctggaaaa
    20041 cctgctaagg aagtgcttct aggcgttact gaagtgttgt cagcaatgac cttcccagtc
    20101 agcatgcact gggctaacaa tagttttgaa tacattcgtc ctgctcacac cttgactgta
    20161 ctcttggatg atgaagctct ggaccttgac ttcttggata ttcattctgg acgcgttagc
    20221 cgtggccacc gtttccttgg aaaagagacg accatcacca gtgcagactc ttatgaggat
    20281 gatttgcgca gccagtttgt tatcgctgat gctaaagagc gtcaagagat gattgtggag
    20341 caaatcaaga cgctggaagt ggagcagggt gtgcaagttg acattgacga agacttgctt
    20401 aatgaagttt tgaacttggt cgagtttcca acagctttca tgggaaattt tgaggctaaa
    20461 taccttgatg tcccagaaga agtattggta acatcaatga aaaaccacca acgttatttt
    20521 gtggtacgtg atcaggctgg tcacttgatg ccaaactttg tatcagttcg taacgggaat
    20581 gatcaagcca ttgaaaatgt tattaaaggg aacgaaaaag ttttggtagc tcgcttagaa
    20641 gatggtgaat tcttctggcg tgaggatcag aaattgcaaa ttgctgattt agttgccaaa
    20701 ttgaccaatg tgaccttcca tgaaaaaatc ggctctcttg ctgaacacat ggatcgtacg
    20761 cgtgtcattg ccgcatcact tgctaaggaa gccaacctat ctgctgagga agtcacagct
    20821 gttgaccgtg ctgcccaaat ttacaaattt gaccttttaa caggtatggt tggtgaattt
    20881 gatgagctgc aagggattat gggtgaaaaa tatgcccgtc tagctggtga agatgctgct
    20941 gtggcaaggg ctatccgaga acactactta cctgatgctg caggaggtgc gctacctgaa
    21001 accaaagttg gcgctgttct ggccttggca gataaattgg atactctctt gtcattcttt
    21061 tctgttggct tgattccatc tggctctaat gatccttatg ctctacgtcg tgcaacgcag
    21121 ggtatcgttc gtattcttga tcattttgga tggcgcatcc caatggataa gctggtagat
    21181 agcttatatg acttgtcctt tgatagtttg acctatgcca acaaggcgga tgtgatgaac
    21241 tttatccgtg ctcgtgtgga taagatgatg ggcaaagccg caccaaaaga cattcgggaa
    21301 gctattctag caagctcaac ctttgtggtt ccagaaatgt tagcggtagc tgaagctctt
    21361 gtcaaagcca gccatacgga aaattacaaa ccagcagttg aatctttgtc acgtgctttt
    21421 aatttggcag aaaaagcgga cgcttctgtt caggtggacc ctagcctctt tgaaaatgag
    21481 caagaaaaca ctttgtttgc agttattcaa ggcttgactc tagcaggcag tgctgcccag
    21541 cagttagaac aggtctttgc tcttagccca gtgattaatg atttctttga taacacaatg
    21601 gttatggcag aagatcaagc ccttaagaac aaccgtttag ctattttatc agatttagta
    21661 agcaaagcta agaccattgc tgcttttaac caactaaaca ccaagtaaat agaaaggaaa
    21721 gagccgttgg ggacttagct agaaaagtga gttcagtacc cgagtggaaa aaacaaggcc
    21781 tttaaacatt gcttttcaca gtgagagtta agtcagtttt ttaagcctta tacttttttc
    21841 ccaggctctt agtggcttaa catggatcct aaaaaaattg ctcgtatcaa tgagctggct
    21901 aaaaagaaaa aaacggtagg cttgactgga cctgaaaaag tggaacaggc aaaactccgt
    21961 gaagaataca ttgaaggcta tcgtcgctct gtcagacacc atatcgaagg catcaaattg
    22021 gtggacgaag aaggaaacga cgtgacccct gaaaaattaa gacaggtgca acgtgaaaaa
    22081 ggcttgcacg gccgttcatt agatgaccca aaatcataag acaaaaagca gctctgaact
    22141 gctttttgtt tatcgtgatg actgttagct tttattttaa atggtatgat acttcaaagt
    22201 gaccacacgt ttttatattg tatagtcaaa atattaggaa aaaccatgtg tttgagttat
    22261 aatagaagaa atcaaagaaa gaaggtagta cttatgacaa ttaaaaaatt ctctctacta
    22321 gccatagcca gcctctcttt actcagtctt gcagcctgtg atatggatga taaagacgat
    22381 catatggata atcaaccaaa gacaagtcaa actagcaaga aagttaaact ttcagaagac
    22441 aaagccaaat caattgcgtt aaaagatgct tctgttacgg aagctgatgc tcaaatgcta
    22501 tcagttacac aagataatga agatggcaaa gccgtgtatg aaatcgaatt tcaaaacaaa
    22561 gatcaagaat acagttatac cattgacgcc aactcagggg atattgtgga aaaatcttct
    22621 gaaccaatca atgactaatc aagaactgta atgaataaga cagccttaac tcttgtgccc
    22681 aattgtcttt gcctcttagg cacggctgtt tgataccgtg ttgcctttga ggcttagttc
    22741 tctttatgac ccaatgatga ctctggatta gaagagtgat tgttgggtct tttgattttc
    22801 tttgataaga aagattacct cgttagaaat gaggagacgt ggttgtcatc agtggatttt
    22861 tcaagatcat ctagtgggat aagctaccta tcttatcagc ttttttttag ctttaaaaat
    22921 tcttgataat atatttctga taaaattagc tataatagaa tcaaatagga aaacgcttac
    22981 ttaaaggagg gtaatgaatg tcgcaagaaa agtatatcat ggctattgat cagggaacaa
    23041 caagttcacg agcgattatt ttcaatcaaa aaggggaaaa ggttagcagt agccaaaaag
    23101 aattccctca gattttccca cacgctggtt gggtagagca caatgctaat cagatttgga
    23161 attcggttca gtctgttatt gcgggtgctt ttattgagtc cagcatcaaa ccaagccaaa
    23221 ttgaagcaat cggaattacc aaccaacgtg agaccacagt ggtttgggac aaaaaaacgg
    23281 gtgtacctat ttataatgct attgtgtggc aatcacggca gacggcacca attgctgagc
    23341 aactcaaaca agatggccat acaaaaatga tccatgaaaa gacaggcctt gttattgatg
    23401 cttacttttc tgcaaccaag atccgttgga ttttggacca tgtgccaggt gctcaagaac
    23461 gagctgaaaa aggcgagtta ctttttggaa ccattgatac ttggctggtt tggaaattga
    23521 cagatggtgc tgttcatgtg acggattatt ccaatgcggc tcgtaccatg ctctataata
    23581 ttaaagactt aacatgggat gatgagattt tagagctttt aaatattcca aaagatatgc
    23641 ttccagaggt taaatccaat tctgaaattt atggcaaaac agctgccttt catttttatg
    23701 ggggcgaagt gcctatttca ggtatggcgg gtgaccaaca ggcagctttg tttggccaac
    23761 tagcttttga gcctggtatg gtgaaaaaca cttatggcac cggttctttc attatcatga
    23821 atactggtga tgagatgcag ttatcgagta ataatctttt gaccaccatt ggttacggga
    23881 ttaatgggaa ggttcactat gccttggaag gttccatctt tattgcaggc tctgccattc
    23941 aatggttgcg agatggtctt aaaatgattg agacttcacc agaatctgaa cagttcgctc
    24001 tagcatcaac tagtgacgat gaagtttatg ttgtgcctgc ttttaccggt ttaggagctc
    24061 cttactggga ttctaatgcg cgtggatctg tctttggctt gacacgtggt accagcaaag
    24121 aagactttgt caaagcaacc ttacaatcta tcgcctatca agtgcgagat gttattgata
    24181 ccatgcaggt ggattcaggt attgacattc aacagctccg tgtggatggc ggtgcagcca
    24241 tgaataatat gctcatgcaa tttcaggctg atattttagg aattgatatt gcccgtgcta
    24301 aaaatttaga aacaacagct ctaggagcag ctttcttagc agggcttgct gtgggctact
    24361 gggaagacat ggatgctttg aaagagttaa atgccacagg ccagctcttt aaggctagca
    24421 tgaatgaatc gcgcaaagaa aaattataca agggttggaa gcgcgctgtt aaagcaactc
    24481 aagttttcac ccaagaagaa gatgcagatg acgatgcaaa ataactagag gaggatggaa
    24541 tggaattttc aaaagagaca agacgcttag ctctccaaaa aatgcaagaa agagatttag
    24601 acctactgat tattggggga ggtattacgg gtgctggtgt ggcgcttcag gcggcagcta
    24661 gtggcctaga tacgggtctg attgagatgc aagattttgc ggaaggaacc tccagtcggt
    24721 cgaccaaatt ggttcatggg ggacttcgtt acttgaaaca gtttgacgtt gaagtcgtct
    24781 cagatactgt ttcagaacgt gctgtggtcc aacagattgc acctcatatt cctaagccag
    24841 atcctatgtt attacctgtt tatgacgaac ctggcagcac ctttagcatg ttccgtttga
    24901 aggtcgctat ggatttgtat gatcttttag caggcgtgtc caatacgcca gcggccaaca
    24961 aggtgttaac caaagaagaa gtcttaaaac gagaaccaga cttaaaacaa gaaggcctgc
    25021 ttggtggtgg cgtttacctt gatttccgca ataatgatgc aaggcttgtt attgaaaata
    25081 tcaaacgagc taatcgtgat ggggctctta ttgctagtca tgtgaaagca gaagatttct
    25141 tgctagatga caatggtaag attattggtg tgaaggcgcg tgatttgctg tcagatcaag
    25201 aaatcattat caaggctaaa ttagtcatca acaccacagg tccatggagt gatgagattc
    25261 gtcaattctc tcataaggga caaccgattc atcaaatgcg ccctacaaaa ggggtgcatc
    25321 tggtagtgga ccgtcaaaaa ttaccagtgt ctcaacctgt ctatgttgac acagggttaa
    25381 atgatggtcg tatggtcttt gtcttgccac gtgaagaaaa aacttatttt ggaacaacgg
    25441 acacggacta cactggagac ttggagcacc cacaagttac tcaagaagat gtggattatt
    25501 tgttaggcgt tgtcaataac cgctttccaa atgccaacgt gaccattgat gatattgaaa
    25561 gcagttgggc tggtcttcgc cccttgttat caggtaatag tgcttctgac tacaatggtg
    25621 gcaacagcgg taaagtcagt gatgatagtt ttgatcactt ggttgatact gtcaaagcct
    25681 atattaacca cgaagatagc cgagaagctg ttgaaaaagc tattaagcag gttgaaacca
    25741 gcacatctga aaaagaattg gatccgtctg cagtgtcacg aggttcaagt tttgaccgtg
    25801 atgagaatgg actctttacc ttggcaggtg gtaagattac cgactatcgc aaaatggctg
    25861 aaggagcatt gacagggatt attcaaatcc tcaaagaaga gtttggcaaa tccttcaagc
    25921 ttatcaattc aaaaacctat cctgtttcag gaggtgaaat caatccagcc aatgtagatt
    25981 cggaaataga agcctatgct caattaggaa ctcttagtgg tctttcgatg gatgatgcta
    26041 ggtatttggc aaacctttat ggttctaatg cgccaaaagt ctttgcctta actcgtcaat
    26101 taacagcagc tgaagggtta agtttagctg aaaccttgtc cttacattat gcgatggatt
    26161 atgaaatggc tcttaaaccg acagattatt tcttgagaag aacaaatcac ctcttattta
    26221 tgcgagatag cctagatgct ttgattgacc cagtgattaa tgaaatggct aaacattttg
    26281 aatggtctga tcaggaaaga gtggcacaag aagacgatct tcgtcgcgtg attgcagaca
    26341 atgatttgag tgccctaaaa ggccatcagg agggttaata tggatatctt tggtgaattt
    26401 ttgggtacag tcttattggt tttactagga aatggtgtgg tagcaggggt tgtcttgccc
    26461 aaaacgaaaa cccatgcttc tggctggatc gtgattgcga ctggctgggg aattgcagtt
    26521 gctgtggcag tctttatcag tggcaaagtt gcccctgcct acctcaatcc agctgttagc
    26581 cttgcttttg ccatgagtgg aaccattgcc tggtcaacag ccattgccta tagccttgcc
    26641 caactattgg gagctatggt tggttcaact ctggtattcc ttcagttcag gccacattat
    26701 ctggctgctg agagtcaggc tgatatttta gggacatttg cgacaggtcc tgctattcga
    26761 gatactagct caaacctatt gagtgaaatc tttgggacct ttgtcttgat gcttggtatt
    26821 ttggcatttg gcttatatga tatgccagca ggactaggaa ccctctgtgt aggtaccttg
    26881 gttattggga ttgggttatc cttaggagga acgacaggtt acgccattaa cccagcgcgt
    26941 gatttaggac ctcgtctagt tcatgctatt ctaccactca acaacaaggg agattctgat
    27001 tggtcttatg cttggatacc tgttgtagga ccaattattg gagcggcctt agcagttttg
    27061 cttttccaag tcatgtccta accatcacta aaggggttgg ctatcatagg ccagctcctt
    27121 tgcattttct ttataggatt aggtaaaacg ctttcatatc ttaaaaacaa ctgctataat
    27181 aatactatta tgacattaac gacaaagacc atacatgtta tcggggcatc ctttgctggt
    27241 ctagcctttg ttgataaata taaggacctc aatccagatt cccaaatcat tctcattgat
    27301 aaggaaagct gtcccaatta tattccaaat ggtatcaacc aattattcag aggagacatt
    27361 caagacctgt ctgatgctat gtggggaaga gcctgcctag cagcacaaat agaatctaac
    27421 caccggatta ttcaagcaga agtgttagct atcgaagctc cctcaaacac cttgcttctc
    27481 aaagacagtc aaggacgagt ttttgaagaa ggttatgaaa cgcttgtttg tgccatgggt
    27541 gctagccccc agtctcatta tattgagacg tcacagacca acaaagtttt ggtaaccaaa
    27601 tattatgaag aaagccaagc tagtttaaaa ctgattgagg ctagccaaga ggtcttggtt
    27661 attggtgccg gcttgattgg cttagacctt gcctacagtt tgtcattaca aggcaaacgg
    27721 gtcaaactga ttgaagcagc cgaacgtcca gatttctatc agactgacgc agaactgata
    27781 gcccctgtaa tggcagaaat gtccactcac catgtcacct ttattaataa taagcgtgtt
    27841 acggctattc atgagataga gggaaaagtt gtcgctcata ccgagcaagg ggacaccttc
    27901 caaggtgatt tggctatttt agccattaac tttaggccaa atacacacct tttgcaagga
    27961 caagtggctt gtgctctgga taagaccatt ttggttaatg aaaacttaca gaccagccag
    28021 gttaatatct atgccattgg agacatggtt tcattgcatt ttggtatatt gggaatggat
    28081 tactatacac ctttgattaa ccaagcaatg aagacaggtc aggctctggc tctgcattta
    28141 gctggatatc ctattcctcc attacagacg gtcaaggtat taggaagttc tcactttggt
    28201 tattatcgcg cgagtgttgg tgtgacagaa gaagaagcag agctttatat ggatacgtgt
    28261 agctatttgt accaaaatgg ggattctaaa aacctctttt ggctgaaatt aatagctaga
    28321 aaaacggatg ggattttaat aggcgctcag ctcctatcca aaaccaatgc tcttgtcatc
    28381 gccaatcaat taggtcaggc tctggctctg aaagttacag acgctgactt agcatttcaa
    28441 gattttctct ttttacaagg tcacagtgat ttggcttatc atcttcatga agcttgtctc
    28501 aaattatttg aaaagaggct ccgccatgaa gattgaagac ttaatggata aggaacgaag
    28561 ggcccaatat cgtctcttgg tgacccttta tcatgctaaa gaaacgttaa ggctaaaaga
    28621 cctaatgagg ctatcaaact tgtctaaagt gaccctatta aaatacattg acaatctaaa
    28681 tcatttgtgt cgagaacaag gtttagcctg tcaattactt ttagaaaaag acagtctgag
    28741 cttaaaagaa aatggccagt tccattggga ggatttggta gctctgcttc tcaaagaatc
    28801 ggttgcctat cagatattga cctacatgta ctgccataaa cactttaaca tcaccaactt
    28861 atccgttgaa ctgatggtta gtgaagctac tctaaacaga caattagctc acctcaacca
    28921 gcttttatca gaatttgatc tggcgctctc gcaaggcaga caactgggaa gtgagcttca
    28981 gtggcgttat ttctattttg aattgttccg ccataccttg accagacaag gcatagacgc
    29041 cttggtcaat cagttagacg cttcgcattt ggctacctta attgagcggc tgattggcca
    29101 gtcattatcg gcagaagcct tagaacaatt attgatttgg ttggcgattt cgcaagcgcg
    29161 aatgtccttt caaaaaggct acaatgatca ttttttaaga gatagtgact ttatgacatc
    29221 taatattttc tttaagcgct tagaaagcat gcttttacat tacttaagac gctatgccct
    29281 tgaatttgat gcttttgaag ctaagagcct ctttgtcttt ttgcatgctt atcctctact
    29341 tcctattgcc agcatgaagt atagtttggg ttttggaggt cctattgctg atcacatttc
    29401 agaagcctta tggcttttaa agaaggctca tgtgattatc catcagacaa aggaggaaat
    29461 catttatggc ttggggatat ttttttccaa agcttacttt tttaaaggag ttatccttag
    29521 tcaaccaacc aatagccaat acctttatca attagtaggc gaagataaaa gagcgctttt
    29581 aagggttatc ataaaccatt tagtgctgca aatggatcag gaaacggatt tcagtcagca
    29641 gttgagtgac gacattttag cattgttgat tttttccatt gagcgccacc atgagcctct
    29701 tttagtaggg cttgccttgg ggcaaaataa ggtggaagca gccattgcgg aattggccat
    29761 tagacgtcat ttggggcatc gtagggattt tcaattaatg ccctatgatc atcagaaagt
    29821 ttatgattgt ctgattacct atcagactgt ctgtttaccg agacaagatt taccctatta
    29881 ccggttaaaa cagtatagct ccccctatga actcactgct ttagaagctt ttctcaaaga
    29941 ccttttccag caaaaaaata cacgagaaga ggaactctca ctcagcccag cagctaagtc
    30001 tagttttgca cacaaaactg tgtgatactt ggtcttttct aaggactgtt acacttttat
    30061 aataataaag taggatagtc taaaggtaga tgatttgaaa aagcttatca aatagagctt
    30121 aacaaaatcg aaaatttttg gaggtaagaa atgaaatttt tcctagatac agctaatgta
    30181 gccgctatta aagcgattaa tgaacttggc gttgtagatg gtgtcaccac caatccaacc
    30241 attatctcac gtgaaggacg tgattttgaa acagttatca aagaaatctg tgacatcgtc
    30301 gatggcccaa tcagtgctga agtgacaggg ttaacagcag atgctatggt agaagaagct
    30361 agaagcattg ctaagtggca tgacaatgtc gtcgttaaaa ttccaatgac tacagaaggg
    30421 ttaaaagcaa caaacattct ttcaaaagaa gggattaaaa ctaatgtgac cttgattttc
    30481 acagtaagcc aaggtttaat ggcaatgaaa gcaggcgcaa cttacattag cccatttatc
    30541 ggtcgtttgg aagatattgg cacagatgct tatcaattaa tcagcgacct tcgtgaaatc
    30601 attgacttgt acgatttcca agcagaaatc atcgcagcaa gtattcgtac aacagcccat
    30661 gtggaagctg ttgctaaatt aggcgcacac attgcgacca ttccagatcc cttgtttgct
    30721 aagatgatgc aacatccctt aacgacaaat ggtttgaaaa cctttatgga agattgggct
    30781 tcctttaaaa aataaggcta aagagtctgt ggctactgtg tcaacaggct ctcttatatt
    30841 catcgtcaaa aagaattcac caatgatgtc caaactagtt tttttctggc aaaataggga
    30901 aaaagaattt agggactttg gaggattttc tgaaaaaagc gtctattttt gtgatacaat
    30961 agacaataga aaacgtttaa ttttagttgt aataaacaga gaggttttac ttatgacatt
    31021 tgatgcaatt gaccaattgg cagtaaacac tgtccgcacc ctatcaatgg atgccattca
    31081 agcggcaaat tctggacacc caggacttcc aatgggggca gcacctatgg cctatgttct
    31141 ttggaatcac ttcatgaaca tcaatcccaa aacaagccgt aattggtcaa acagagaccg
    31201 ttttatccta tcagcaggtc atggaagtgc catgctttat agcttgttgc atttagcagg
    31261 ttatgattta tctgtagaag atttaaagaa cttccgtcaa tggggttcta aaacaccagg
    31321 tcacccagaa gtgaaccaca cagacggtgt cgaagcaacc acaggacctc ttggtcaagg
    31381 gatcgcaaat gccgttggta tggccatggc agaagctcac ctagcagcta aatttaacaa
    31441 accaggcttt gacatcgttg atcactacac atttgctttg aatggtgacg gtgaccttat
    31501 ggaaggggtc agccaagaag cagcaagtat ggcaggacat ttaaaacttg ggaaattggt
    31561 cttgctatat gattcaaacg acatctctct tgatggtcca acctctatgg ctttcacaga
    31621 agatgtgaaa ggacgtttcg aagcttacgg ttggcaacat atccttgtga aagatggaaa
    31681 tgatttagaa gaaattgcag ctgctattga agcagctaaa gctgaaactg agaaaccaac
    31741 catcatcgaa gtcaaaacca ttattggttt tggtgccgaa aaacaaggaa cttcagctgt
    31801 tcacggcgct cctcttggag cagaaggcat tgcttttgcc aaaaaagctt accaatggac
    31861 tcatcaagat tttgaagtgc ctgctgaagt aacagagcgc tttgctcaag gtcttcaagc
    31921 gcgtggtgaa aaagcagaac aagcctggaa tgacttgttt gcagcttatg aagcagaata
    31981 tccagaatta gcagcagaat accaaaaagc ctttactaac gaagctgctc aagtagaact
    32041 tgaagcgcac gaacttggta gttcaatggc gagtcgtgta tccagtcagc aagctatcca
    32101 acaaatttca gaacaagtag cctctttctg gggtgggtca gcagaccttt cagcttcaaa
    32161 caatactatg gttaaggctg aaacagattt ccaaccaggt cactacgaag gccgtaacat
    32221 ctggtttggc gttcgtgaat ttgccatggc tgcagccatg aacggtattg cccttcacgg
    32281 cgggacacgc gtttacggcg gaaccttctt tgtcttctca aactatcttc ttccagctgt
    32341 tcgtatggca gcgctccaaa acttgccaac agtttatgtc atgacacatg attctatcgc
    32401 tgtcggtgaa gatgggccaa cgcacgaacc aattgaacaa ttggcaagtg ttcgctcaat
    32461 gcctaacttg aacgttattc gtccagcaga cggtaacgaa acaaatgctg cttggaaacg
    32521 tgccattgct gaaacagatc gcccaaccat gcttgtattg acgcgtcaaa accttccagt
    32581 gcttgagggc acaaaagaat tggcagaaga tggtcttaac aaaggagctt atatcttgtc
    32641 agaagccaaa ggcgaccttg atggtatcat cattgcaaca ggttcagaag tgaaactggc
    32701 aatggatact caagaagctc ttgaagcaga agacattcat gtgcgtgtcg tatctatgcc
    32761 atcacaaaat atctttgacg aacagtcagc agagtataaa gaaagcatcc taccagcagc
    32821 tgtgactaaa cgtctcgcta tcgaagcagg ttcaagcttt ggctgggcaa aatatgtcgg
    32881 cctagcagga aaaacattga ccattgacac ttggggtgct tcagctccag gaaatcgtat
    32941 cttcgaagaa tatggtttca ctgtggcaaa tgcaactgag ttatacaaat cactctaggt
    33001 tacacatccc aaacagagta aatcatttga tttactctgt ttttttaaaa taaaatactc
    33061 agttctttct ttt
//
LOCUS       DATURV010000012        32993 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DATURV010000012 DATURV010000000
VERSION     DATURV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32993
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.012"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            18..98
                     /locus_tag="VCF75_000567"
     tRNA            18..98
                     /locus_tag="VCF75_000567"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Tyr,seq:gta)
     gene            105..175
                     /locus_tag="VCF75_000568"
     tRNA            105..175
                     /locus_tag="VCF75_000568"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Trp,seq:cca)
     gene            187..259
                     /locus_tag="VCF75_000569"
     tRNA            187..259
                     /locus_tag="VCF75_000569"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:His,seq:gtg)
     gene            268..339
                     /locus_tag="VCF75_000570"
     tRNA            268..339
                     /locus_tag="VCF75_000570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Gln,seq:ttg)
     gene            359..442
                     /locus_tag="VCF75_000571"
     tRNA            359..442
                     /locus_tag="VCF75_000571"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Leu,seq:caa)
     gene            1525..1833
                     /locus_tag="VCF75_000572"
     CDS             1525..1833
                     /locus_tag="VCF75_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEP2546157.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDVPYCLLSGCAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            1888..2100
                     /locus_tag="VCF75_000573"
     CDS             1888..2100
                     /locus_tag="VCF75_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEP2546158.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATALMIGSGKTVFALCQTEKQTIIV"
     gene            2210..2653
                     /locus_tag="VCF75_000574"
     CDS             2210..2653
                     /locus_tag="VCF75_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEP2546159.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2657..3376
                     /locus_tag="VCF75_000575"
     CDS             2657..3376
                     /locus_tag="VCF75_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP2546160.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRRLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            3369..4184
                     /locus_tag="VCF75_000576"
     CDS             3369..4184
                     /locus_tag="VCF75_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP2546161.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVIGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4224..4607)
                     /locus_tag="VCF75_000577"
     CDS             complement(4224..4607)
                     /locus_tag="VCF75_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP2546162.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWEGITINQENWDLAPFLKHL"
     gene            complement(4658..5914)
                     /locus_tag="VCF75_000578"
     CDS             complement(4658..5914)
                     /locus_tag="VCF75_000578"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEP2546163.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHSEEVYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6006..8318
                     /locus_tag="VCF75_000579"
     CDS             6006..8318
                     /locus_tag="VCF75_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP2546164.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQKHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEISVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8582..12148
                     /gene="rpoB"
                     /locus_tag="VCF75_000580"
     CDS             8582..12148
                     /gene="rpoB"
                     /locus_tag="VCF75_000580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP2546165.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12239..15880
                     /gene="rpoC"
                     /locus_tag="VCF75_000581"
     CDS             12239..15880
                     /gene="rpoC"
                     /locus_tag="VCF75_000581"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEP2546166.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDNQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTADKYFLEPGQDIQEVIDGLEINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            16032..16397
                     /locus_tag="VCF75_000582"
     CDS             16032..16397
                     /locus_tag="VCF75_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEP2546167.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            16490..17428
                     /gene="tadA"
                     /locus_tag="VCF75_000583"
     CDS             16490..17428
                     /gene="tadA"
                     /locus_tag="VCF75_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEP2546168.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGHES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYARLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            17412..18398
                     /locus_tag="VCF75_000584"
     CDS             17412..18398
                     /locus_tag="VCF75_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEP2546169.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFTEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQGLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMAILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            18400..18726
                     /locus_tag="VCF75_000585"
     CDS             18400..18726
                     /locus_tag="VCF75_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEP2546170.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            18701..19129
                     /locus_tag="VCF75_000586"
     CDS             18701..19129
                     /locus_tag="VCF75_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEP2546171.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            19134..19370
                     /locus_tag="VCF75_000587"
     CDS             19134..19370
                     /locus_tag="VCF75_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEP2546172.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEITLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            19363..19797
                     /locus_tag="VCF75_000588"
     CDS             19363..19797
                     /locus_tag="VCF75_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEP2546173.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRNHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            19781..20107
                     /locus_tag="VCF75_000589"
     CDS             19781..20107
                     /locus_tag="VCF75_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEP2546174.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVSFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            20205..21158
                     /locus_tag="VCF75_000590"
     CDS             20205..21158
                     /locus_tag="VCF75_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP2546175.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVTLITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            21217..22413
                     /locus_tag="VCF75_000591"
     CDS             21217..22413
                     /locus_tag="VCF75_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEP2546176.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEHIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            22600..22908
                     /locus_tag="VCF75_000592"
                     /pseudo
     CDS             22600..22908
                     /locus_tag="VCF75_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22991..23761)
                     /locus_tag="VCF75_000593"
     CDS             complement(22991..23761)
                     /locus_tag="VCF75_000593"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEP2546177.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(23809..24876)
                     /gene="pepA"
                     /locus_tag="VCF75_000594"
     CDS             complement(23809..24876)
                     /gene="pepA"
                     /locus_tag="VCF75_000594"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEP2546178.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPNIEDIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            25332..25625
                     /locus_tag="VCF75_000595"
     CDS             25332..25625
                     /locus_tag="VCF75_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEP2546179.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            25622..25939
                     /locus_tag="VCF75_000596"
     CDS             25622..25939
                     /locus_tag="VCF75_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP2546180.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWNIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            25957..26583
                     /locus_tag="VCF75_000597"
     CDS             25957..26583
                     /locus_tag="VCF75_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEP2546181.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPIVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            26735..27130
                     /locus_tag="VCF75_000598"
     CDS             26735..27130
                     /locus_tag="VCF75_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP2546182.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISVVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(27384..28025)
                     /locus_tag="VCF75_000599"
     CDS             complement(27384..28025)
                     /locus_tag="VCF75_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEP2546183.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYEVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(28045..29022)
                     /gene="dusB"
                     /locus_tag="VCF75_000600"
     CDS             complement(28045..29022)
                     /gene="dusB"
                     /locus_tag="VCF75_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEP2546184.1"
                     /translation="MIKLNSSFRIGDVEIPRRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(29009..29881)
                     /gene="hslO"
                     /locus_tag="VCF75_000601"
     CDS             complement(29009..29881)
                     /gene="hslO"
                     /locus_tag="VCF75_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEP2546185.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISYLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEAIINDKA"
     gene            complement(30028..31521)
                     /gene="rofA"
                     /locus_tag="VCF75_000602"
     CDS             complement(30028..31521)
                     /gene="rofA"
                     /locus_tag="VCF75_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEP2546186.1"
                     /translation="MIEKYLESSIESKCQLIVLFFKTSYLSITEAAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALVPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSRDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFISPYYKGNQKLYTSLKLIVEEWMAKLPGKRYLNHKHFHLFCHYVEQILRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSINFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVQHELTKGIAVAEISFDESILSIQELIYQVKEEKFQADLTKQLT"
     gene            31753..>32993
                     /gene="sfbI"
                     /locus_tag="VCF75_000603"
     CDS             31753..>32993
                     /gene="sfbI"
                     /locus_tag="VCF75_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
                     /protein_id="HEP2546187.1"
                     /translation="MVSSYMFVRGEKMNNKMFLNKEAGFLAHTKRKRRFAVTLVGVFF
                     MLLACAGAIRFGQVAYAADERTVPNFKSPDPDYPWYGYDAYTGAFLRYHDLRVNLNGS
                     RSYQVYCFNIKKNYPRPFTSSDKKWYKRLEGTAETFKVHAMAPRVGGEELTKKLRSVM
                     YNGYPNDGNNIMKGLEPSNAIEVTQYAVWYYSDKSQYEIETLWKSEAKEGKISESQVT
                     LMREALRKLISSDEDLVKQVPSNFKLSTFESSDKSYQNLLSAEFVPDDPPKPGDTSEH
                     NPKTPELDGSPIPEDPKHPDDNLEPTLPPVMLDGEEVPEVPSESLEPALPPLMPELDG
                     EEVPEKPSVDLPIEVPRYEFNNKDQSPLAGESGETEYITEVYGNQQNPVDIDKKLPNE
                     TGFSGNMVETENTKEPEVLMGG"
BASE COUNT         9927 a         5927 c         7152 g         9987 t
ORIGIN      
        1 aaaatttaat attttacgga aagatagcga agaggctaaa cgcggcggac tgtaaatccg
       61 ctccttcggg ttcgggggtt cgaatccctc tctttccatc ctaaacgggc atagtttaaa
      121 ggtagaacta aggtctccaa aaccttcagt gtgggttcga ttcctactgc ccgtgttaga
      181 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      241 tcgattccca tcactcgcct attttattgg ggtatagcca agcggtaagg caagggactt
      301 tgactccctc atgcgttggt tcgaatccag ctaccccagt ttaaatttta tatttaaagc
      361 cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc agtgtccgca aggacgtgcc
      421 ggttcgaccc cggccgccgg tatacttgaa aaacaaggtt tgtaaacctt gttttttgtt
      481 ttgatcttaa gctgtagcta cgtagtacaa tctcttatat tactgataag gtctatttaa
      541 tgcgcaagat ttgtggtatg atgaatatga ttgagtattg atgctttatg ggattaccgg
      601 taaggttttg aaaaaggttt gtcactacta ggtgcttttt tagctataga aagacagtta
      661 tggattgatg tattgtaact aatgttggag gttaatggtg gttgagtttt gagatattta
      721 cactgataat cgggtggaaa ctggtcgggt atcgggtaat ggagtgtgat tcttcgttta
      781 agcaaggaga ttatcgtttg gtggttcata tttgcttgtt aaatgttagg gatgagatgc
      841 tgagtcagca aggccatgaa gataaagctg actggccaga gctttgagat gtgacgtttg
      901 gtggaagtcc tttttggtga gatggtacag catatagtaa tattagatcc tgaggaagaa
      961 ttgggacttt cattaggctt gactggggta tgacctcact ttacaattca tttcgatgat
     1021 gggtttgatg atacgttttt agtttaagac tttgtagagt tggagaattt ggttctttat
     1081 gaggaagaag tgcaggtaat tcgttgagcc agtcgtgaag atattttagg tatgattgat
     1141 gaaggttcgt tcattcctta tctgaagtat aaaataaatt tgtgctttga gatggttggc
     1201 caatataaag tccatcaatc ttagaagtaa taatttttga attttttctg aggtttgtgt
     1261 atatttgata gtgtactgat gtaagaagta aggagtacta tgatggcaat caatgagaga
     1321 gcttctgcaa aaatcaagtt aggccttgat actaagaata agcgatgaga tggttaccac
     1381 gatttatcga tggtcatgat gagtattgat ctgtgtgact atgtgacggt tgaccatata
     1441 gatgacaata agattgtctt tgcctcaaac tgtccgaaaa tacctattaa ctaagataat
     1501 gatgtttata agatagttca gctaatgaaa catcgatttc aagttaaaag aggagtttcc
     1561 gtttacttgg aaaagcggat tcccatgtgt gctggtatgg gaggaggttc tagcgatgct
     1621 gttactatca gagcattaaa tcagttatgg ctccttactc ttagccgaaa agatatgatg
     1681 gatattggta taccgattgg tagtgatgtg ccttattgct tgctttcagg ttgtgcccaa
     1741 gtcactggta aaggtgaagt ggtttgtcgc attttggggc tgttatcgtc ttgggtggtt
     1801 ttggttaaac cagattttgg tatctcgact tgaacatttt ttctagatat taattgtaaa
     1861 atcatttcaa gagtgtctac tactcatttg gttgcggcga ttgaagcggg taactataat
     1921 gatgggatat tgactgaaat gaacaatttg ctggaagata ttttcattgc caagcgaccc
     1981 tttattcaaa aaataaaaga gaaaacactg caagccggtg ctgctactgc tttgatgata
     2041 ggaagtggaa agactgtttt tgctttgtgc caaaccgaaa aacaaactat aatagtttaa
     2101 aaggactttg caaggaagtt tttaaggtga gaatgttgta atattttgtt gggattgctt
     2161 ctttggaagc ttcatgttat aattaactgg ttaatgaaag aggagattta tggggacttt
     2221 agaaaaaaaa cttgataact tagtaaatac tattttatta aaagcagaaa atcagcatga
     2281 gttattattt ggagcttgtc aaagtgacgt taagcttact aatacgcaag aacatatttt
     2341 aatgttacta tctcagcaac gtctcactaa tacagatttg gctaaggcat taaatattag
     2401 tcaggcggca gtaactaagg ctatcaagag tttggtcaaa caagacatgc tagcaggaac
     2461 taaggatacg gttgatgcta gggtgactta ttttgaatta accgagttag ctaagcctat
     2521 tgcatcagaa catacccatc atcatgatga aaccttaaat gtttacaacc gtttattaca
     2581 aaaattctcc gcgaaagaat tagagattgt agataagttt gtaacagttt ttgctgagga
     2641 attagaaggg taatccatga gatacatatc agtgaaaaat ctctcctttc aatatgaaag
     2701 tgagccagtt ttagaaggga tcacttatca tttagatagt ggagaatttg tcaccatgac
     2761 cggtgaaaat ggcgctgcaa agtcaacctt aataaaagca actttaggaa ttttacaacc
     2821 aaaggctgga cgagttacta ttgctaaaaa aaataaagac ggtaaacaat taaggattgc
     2881 ttacttgccg cagcaagtag ctagctttaa cgctggtttt ccatccaccg tttacgagtt
     2941 tgtcaaatca ggtcgctacc cacgtagtgg ttggtttaga cgtttgaaca aacacgatga
     3001 agagcatgtg caagcaagct tagaagcagt cggcatgtgg gaaaaccgtc ataagagaat
     3061 tggtagttta tcaggtggtc aaaaacaacg tgtggttatt gcccgtatgt ttgcttctga
     3121 ccctgatatt tttgtgctag acgagccaac aacgggaatg gatagcggta ctactgatac
     3181 cttttatgaa ctgatgcacc acagtgcaca tcaacatggg aaatccgttc tgatgattac
     3241 ccatgaccca gaagaagtga aggcttatgc tgatcggaac attcatttag tcagaaacca
     3301 aaaacttcct tggcgttgtt tcaacattca tgaagttgaa acagatgacg aaaaaggagg
     3361 tcatggtcat gcttgatatt ttatcctatg atttcatgca acgggcggta atggcggtag
     3421 ttgccattag tatttttgct ccgattttag gtattttcct tattttacgt cgtcaaagtt
     3481 tgatgagcga tacccttagt catgtttctt tggctggggt agcgcttggg gtagtcattg
     3541 gtatttcacc aaccatcact actattattg ttgtggtttt agctgctatt ttgttagaat
     3601 acctgcgtgt agtttacaaa cactacatgg agatttcaac ggcgattttg atgtcacttg
     3661 gtttggccct atctctgatt attatgagta agtcgcatag ttcatcaagc atgagtttag
     3721 aacaatacct ttttggatcg atcatcacga ttagtatgga acaagttgtc gccttgtttg
     3781 ctattgctgc gattatttta atcttgaccg ttctcttcat tagaccgatg tacattttga
     3841 cctttgatga agatactgct tttgtagatg gtttgcccgt tcgcttgatg tctgttctat
     3901 tcaatatcgt cactggggtt gctattgctt tgaccattcc agcagcagga gcacttttgg
     3961 tttctaccat tatggtcttg ccagcaagta tcgcaatgag attgggtaaa aactttaaaa
     4021 cagttatctt actgggaatt gtcatcggtt ttagcggtat gttatctggt attttcttat
     4081 cttatttctt tgaaacacca gctagtgcca ctattaccat gattttcatt agtattttcc
     4141 tcttagttag tctaggtgga atgcttaaaa aacggttatt ttaataagaa aaatcctcac
     4201 atgtttaaca tgtgaggatt tatttataaa tgctttaaaa agggggcaag gtcccaattt
     4261 tcttgattaa tggtgatgcc ctcccaaaag ctatcattag ttaaacgagg aatcaaatga
     4321 aggtgaaaat gagtcccctc atccatcaac tccttgtcat tgctgacgct agtgacgcct
     4381 gaaatgggaa ggtgctgctc tagcttagca actagagcgg cttgtaagtc gatgagatca
     4441 tagcgcactt cctttggtag ttgtgttaga ctagtgtaat ggtctttgga aatcagtaaa
     4501 aggtgcccct tttggatagg atcaatatca aaaacaacct tgaaatattg actttgagtc
     4561 agtagctgat ttgctttgag ctgatggcaa aagatacatt ctgtcatagg tacatctcct
     4621 tatgaaaaaa cgagccagag ctcgtttaat cggttatcta gtaagtgaga acagcgtatt
     4681 ttttcttacc gcggcgaata acggttaatt gatcatcaat tttatcatca ttacttaatt
     4741 ggtaatctaa atcttgaacg cggtctccgt taatgtagat tgctccattt tgcacgtctt
     4801 cacgcgcttg gcgttttgat ggggaaatac ctgcggtgac aagcatgtca acaaggttaa
     4861 ggctgtcttc tgattgaacg tgataatttg gtacattgct caatccttgt tttaattcat
     4921 tagcagaaag atttttgatg tttccagcaa ataactgctc ggtaatgtta agagcttgtt
     4981 tataaacttc ttcgctatga accaaggtaa ccacttcgcg tgccaaggtt ttttgagcga
     5041 gacgctcgtg acgagcggca ttaaattgag tttcaatttc tgcaatctca tctagagata
     5101 agaaggtgaa gattttcaag aaacgcactg catcatcatc catgacattt aaccagaatt
     5161 ggtacatctc gtaaggagac gtcttatcgg catcaagcca gacagcatta ccttctgatt
     5221 taccgaattt tttcccggtt gagtcagtga tgagtggtac agtcatgacg tgcccagttt
     5281 tatcagcctt tttgcggagc aattcagtac cagctgtcat attaccccac tggtcagagc
     5341 cgccaatttg taaggttaca ttatgcttgt cattgagttc gtagaagtcg tagccctgca
     5401 tgatttggta agcaaactca gtgtaagaaa tgcctgtttc aatgcgtttt ttaacagagt
     5461 ctttactcat catgtagtta acggtgaagt atttaccgac atcacggagg aagtcaataa
     5521 agctgatttg cgagaaccag tcgtagttgt tgacaagctc tgctttatta tcaccatttt
     5581 caaaatcaag gaaagtagac aactgccctt taatcttgtc gctccactcc aaaactgttt
     5641 ctttggtttg aaggctgcgc tctgcatctt taaaggaagg atcaccaatt aaacctgtgg
     5701 caccacccac aagagcgtaa ggtttgtgcc ctgctagttg caagcgacga gatgttaaga
     5761 tagccactag atggcccaga tgaaggctgt cagcggttgg atcataaccg gtataatagg
     5821 atacttgccc ttctgttaat gctttgacaa gggcttgttc atcagtcgtt tgaaagacca
     5881 agccacgagc tttgagttct tcaaaaatat tcataggctt tctccatttt cattatagtt
     5941 tcgaatcatt atatcaaaaa acgctactgc tatgcaagct ttatcaaagt ttggtataat
     6001 gataaatgag gtattttatg gtgaaatgga acacgaaaca aaagcgtata agtcatcaaa
     6061 gattaggtct cttggatttg gggccggtgc tattacgtac gttgagacta ctgtctaact
     6121 ttttttatat tgttatcttc ctttttggaa tgatgggatt tggtatggca tttgggtatt
     6181 tggctagtca gattgaatct gttaaggtac caagtaaaga aagtttagtc aaacaagttg
     6241 aatcattaac gatgatttcg caaatgaact attctgataa tagtttaatt tctactttag
     6301 atacggattt acttcgaaca ccagtagcta atgatgcgat ttcagagaat atcaaaaaag
     6361 ctattgtatc aacagaagac gaacattttc aaaaacataa aggtgtcgtg ccaaaagctg
     6421 tttttcgggc aacattggct tctgtattgg gatttggaga agctagtgga ggttcgacct
     6481 taacacagca attggtcaaa caacaagttt tgggagacga tcccacattt aagcgcaagt
     6541 ctaaggagat cgtttacgct cttgccttag agcgttatat gtccaaagac aatatcttat
     6601 gtgattatct taatgtttca ccttttggtc gtaataacaa gggccaaaat attgctggtg
     6661 ttgaagaagc tgcgcgtggc atttttggcg tttctgccaa agatttaacg gtgccacagg
     6721 cagcattttt ggcggggctt ccgcagagcc ctattgttta ctctccttat ttgtcaacgg
     6781 gacaactgaa gtcagaaaag gacatggctt atggcatcaa gcgtcagcaa aatgttctct
     6841 ataacatgta ccgtacaggt gttctgtcta aaaaagaata cgaggactat aaggcttatc
     6901 cgattcaaaa ggattttatt caaccgggaa gcgcaatagt aaataatcac gattaccttt
     6961 attacacggt gttagcggat gctaaaaaag ccatgtatag ctatttgatt aagcgagata
     7021 aggtgtctag tcgtgacttg aaaaatgacg agactaaggc tgcttatgaa gagagagcct
     7081 taacagaatt acaacagggt ggctatacca tcaccacaac cattaataag cctatttaca
     7141 atgcgatgca gacagcggca gctcagtttg gtggcctgtt agatgatggc actggcacag
     7201 ttcaaatggg aaatgtcttg acagacaatg cgactggtgc tgtgttaggt tttgttggtg
     7261 gtagagatta tgctctgaat caaaataatc atgctttcaa tacagttaga tcgccaggtt
     7321 ctagcattaa accgataatc gcttatggtc ctgctattga tcaaggttta atggggagtg
     7381 ctagcgtttt gtctaattac ccaacaactt actcgagtgg ccaaaaaatc atgcatgctg
     7441 atagtgaagg aacagccatg atgccacttc aagaggccct aaatacttct tggaacatcc
     7501 cagctttttg gacacagaaa ttactgcgtg aaaaaggggt cgatgtcgaa aattatatga
     7561 caaaaatggg ttataagatt gcagactatt cgattgaaag tttacctcta gggggcggta
     7621 ttgaaatctc ggttgctcaa caaaccaatg cttaccaaat gctttcaaac aatggcttat
     7681 atcaaaagca atatattgta gataagatta ctgctagcga tggtacagtc gtttacaaac
     7741 atgaaaataa gccaattcgt attttttctg cagcaacagc tacgatttta caagaattgt
     7801 tgagaggtcc gattacttca ggcgctacga ctactttcaa gaaccgtttg gcggctatta
     7861 atccgtggct tgctaatgct gattggattg gtaagaccgg aacaactgag aattatacgg
     7921 atgtttggct agtcctgtct actccaaaag ttaccttagg cggttgggca ggacatgatg
     7981 acaatacctc attagcgcca ttaacaggat ataacaataa ttctaattat cttgcctatt
     8041 tagctaatgc cattaatcag gccgatccca atgttattgg agtagggcaa cgcttcaact
     8101 tagatccagg agtcattaag gcgaatgtct tgaagtcaac aggtttacaa ccaggaactg
     8161 ttaatgtcaa tggacatact ttttctgttg gcggagaaat gaccaccagt ctatggtccc
     8221 aaaaaggacc gggggctatg acttaccgat ttgctattgg tggcacggat gccgattatc
     8281 aaaaagcctg ggggaacttc gggttcagaa aaaattaggt tgccatttat cgataaaaag
     8341 ggtataatag taataactat ttgtcgtcca atctagctga aatattgtcc agttaggaag
     8401 aacagcagtt aaatcacact gataaagtca gatttagctg ctctttttgt gtctattttt
     8461 agaaaagata gggtttgtaa cctatattta aatattctaa aaattaacat ttagggcaaa
     8521 tgataactta gttgcgattt gctgaaacag caaagcttaa atagaagaag gagctaaaaa
     8581 cttggcagga catgaagttc gatacggaaa acaccgtaca cgtcgtagct tttcaagaat
     8641 caaagaagtt cttgatttac caaatttgat tgaaattcaa actgactcat tccaagattt
     8701 cctcgattca ggtttgaaag aagtatttga agatgtactt cctatttcaa actttacgga
     8761 tactatggaa cttgaatttg ttggttacga atttaaagaa cctaaatata cccttgaaga
     8821 agctcgtatc cacgatgcaa gttattctgc accaatcttt gtgaccttcc gcttggtcaa
     8881 taaagaaact ggtgaaatca agacacaaga agttttcttc ggtgacttcc ctatcatgac
     8941 ggaaatgggt acttttatca tcaatggtgg tgaacgtatc atcgtttctc agttggtgcg
     9001 ttctccaggt gtttatttca acgataaagt tgataaaaac ggtaaagttg gttatggatc
     9061 aacagtgatt cctaaccgtg gggcttggtt ggaattagag actgactcaa aagacattgc
     9121 ctacactcgt attgaccgta cccgtaagat tccattcaca accttggttc gtgcccttgg
     9181 tttctcaggt gatgatgaaa ttgttgatat ctttggtgaa agcgaccttg ttcgtaatac
     9241 cattgaaaaa gacattcaca aaaatccaag tgattctcgt acagacgaag cccttaaaga
     9301 aatttacgag cgtcttcgtc caggtgagcc aaaaactgca gatagctctc gtagtctttt
     9361 gattgcgcgt ttctttgatg cacgtcgtta cgatttggct gcggttggtc gttacaaagt
     9421 gaacaaaaaa cttaatatca agactcgtct tttgaaccaa atcattgctg aaaaccttgt
     9481 ggatgctgaa actggcgaaa ttttggtaga agctggaact gagatgactc gtagcgtcat
     9541 cgaatccatc gaagaacacc ttgacggtga tttgaacaag tttgtttaca caccaaatga
     9601 ttacgctgtg gttactgaac ctgttgttct tcaaaagttc aaggttgtgt caccaattga
     9661 tcctgatcgt gtagtaacta ttgttggtaa tgccaatcca gatgataagg ttcgtgcatt
     9721 gacacctgct gatattttgg cagaaatgtc atacttcttg aaccttgctg aaggtcttgg
     9781 aaaagttgac gatattgacc acttaggtaa ccgtcgtatc cgtgccgttg gtgaattgct
     9841 tgccaaccaa ttccgcatcg gtcttgctcg tatggagcgt aacgttcgtg agcgtatgtc
     9901 tgttcaagac aacgatgtgt taacaccaca acaaatcatc aatatccgtc ctgtcacagc
     9961 agctgtcaaa gaattcttcg gttcgtctca gttgtcacag ttcatggacc aacacaaccc
    10021 attgtcagag ttgtctcaca aacgtcgttt atctgcctta ggacctggtg gtttgacacg
    10081 tgaccgtgct ggttatgagg ttcgtgacgt gcattacacg cactatggcc gtatgtgtcc
    10141 gattgaaaca cctgaaggac caaatattgg tttgattaac aacttgtctt catttggaca
    10201 tcttaataaa tatggtttca ttcaaacacc ttaccgtaag gttgaccgtg cgactggtag
    10261 ggtaactaac gagattgttt ggttgactgc cgacgaagaa gacgaataca cagttgcaca
    10321 ggccaattcg aaactaaacg aagatggcac ttttgctgaa gaaatcgtta tgggtcgtca
    10381 ccaaggtaat aaccaagaat tttctgcaag tgttgttgat ttcgttgacg tttcccctaa
    10441 acaggtagtt gctgttgcga cggcatgtat tcctttcttg gaaaatgatg actccaaccg
    10501 tgctctcatg ggtgccaaca tgcaacgtca ggctgtgcca ttgattgatc caaaagcacc
    10561 atatgttggt actggtatgg aatatcaagc tgcccatgac tcaggcgctg cggtgattgc
    10621 tcagcacaat ggtaaagttg tcttttctga tgctgaaaaa gtggaaatcc gtcgtcaaga
    10681 tggctcactt gatgtttacc acattaccaa attccgtcgt tcaaactcag gaacagccta
    10741 taaccaacgc acccttgtta aagtaggaga catcgttgaa aaaggtgatt tcatcgctga
    10801 tgggccttct atggaaaatg gtgaaatggc ccttggacaa aacccagtcg ttgcttacat
    10861 gacttgggaa ggttacaact tcgaggatgc tgttatcatg agtgagcgcc ttgtgaaaga
    10921 agatgtctac acatctgttc acttggaaga attcgaatct gaaacgcgtg atacaaaact
    10981 tggccctgaa gaaatcactc gggaaatccc aaatgttggt gaagaagccc tcaaagacct
    11041 tgacgaaatg ggaattatcc gtatcggtgc tgaggttaaa gaaggcgaca tcttagtagg
    11101 taaagtcaca cctaaaggtg aaaaagacct ttctgctgaa gaacgtttac ttcacgccat
    11161 ctttggggat aaatcgcgtg aagtgcgtga tacctcactt cgtgtccctc atggtggtga
    11221 tggtattgtt cgtgatgtga aaatctttac acgcgctaac ggcgatgaat tgcaatcagg
    11281 tgttaatatg cttgtgcgtg tttacatcgc tcaaaaacgt aaaatcaagg tcggagataa
    11341 aatggccggt cgtcacggaa acaagggtgt cgtttcacgt attgtaccag ttgaagacat
    11401 gccatacctt ccagacggaa caccagttga catcatgttg aaccctctcg gggtgccatc
    11461 acggatgaat attggtcagg ttatggaact tcaccttggt atggctgctc gtaatcttgg
    11521 cattcacatt gcaacacctg tctttgacgg ggcttcatca gaagaccttt gggacactgt
    11581 tcgtgaagct ggcatggata gcgatgctaa gacggtcctt tatgatggtc gcaccggtga
    11641 accatttgac aaccgtgtat ccgttggtgt catgtatatg atcaaacttc accacatggt
    11701 tgatgataaa cttcatgccc gttctgtagg accatactca cttgttaccc aacagccact
    11761 tggtggtaaa gctcaatttg gtggacaacg ttttggtgag atggaggttt gggcccttga
    11821 agcttatggt gcatcaaatg ttcttcaaga aatcttgacc tacaagtcag atgacgtcac
    11881 aggacgtttg aaagcctatg aagcgattac taaaggtaaa ccaattccaa aaccaggtgt
    11941 accagaatcc ttccgtgttc ttgtaaaaga attgcaatcg cttggtcttg atatgcgtgt
    12001 gcttgacgag gatgataatg aagtggaact tcgtgatctt gatgaaggtg aagacgatga
    12061 tattatgcat gttgacgatc tcgagaaggc acgtgaaaaa caagctcaag aaacccaaga
    12121 agtttctgaa acaactgacg aaaaataagc aatcaattct tattaaataa ttatttactg
    12181 gtctggggca aaggccccag gaactggtaa agtcatcaaa ggcagaaagg taaaactagt
    12241 ggttgacgta aatcgtttta aaagtatgca aatcacatta gcctcaccaa gtaaggtccg
    12301 ttcatggtct tatggtgaag ttaaaaaacc tgaaacaatc aactaccgta cattaaaacc
    12361 agaacgtgaa ggactctttg acgaagtcat ctttggtcca acaaaagact gggaatgtgc
    12421 gtgtggtaag tacaaacgta tccgttataa agggatcgtt tgtgaccgct gtggggttga
    12481 ggtaactcgt gctaaagtac gtcgtgaacg tatgggtcac attgagttaa aagctcctgt
    12541 atcacatatc tggtatttca aagggattcc atctcgtatg ggattgactc ttgatatgag
    12601 tcctcgtgcc cttgaagaag tgatctattt tgcggcttat gtggtcattg atccaaaaga
    12661 tacaccgctt gaaccaaaat cattattaac agaacgtgaa taccgtgaaa aactacaaga
    12721 gtatggtcat gggtcatttg tcgctaagat gggtgctgaa gccattcaag atctcttgaa
    12781 acgtgtggac ttggcagctg aaatcgctga attaaaagaa gagttgaaat ctgcttctgg
    12841 tcaaaaacgg attaaagcag ttcgtcgttt agacgtcctt gatgccttta acaaatctgg
    12901 aaataaacca gaatggatgg ttcttaacat cctgccggtt attccaccag atcttcgtcc
    12961 gatggttcaa ttggatggtg gtcgttttgc ggcatcagac ttgaatgact tgtaccgtcg
    13021 tgtgattaac cgtaataacc gtttggcacg tttgttagaa cttaatgccc ctggcatcat
    13081 tgttcaaaat gaaaaacgaa tgcttcaaga agccgttgat gctttgattg ataatggtcg
    13141 tcgtggtcgt ccaatcactg gaccaggaag tcgtccattg aaatcattga gccacatgct
    13201 taaaggtaaa caagggcgtt tccgtcaaaa cttgcttggt aaacgtgtag acttctctgg
    13261 acgttccgtt atcgctgttg gtccaaccct taaaatgtat caatgtggtg tcccacgtga
    13321 aatggctatc gagcttttta aaccatttgt aatgcgcgaa attgttgcca aagaatatgc
    13381 tggtaacgtt aaagccgcta aacgtatggt tgaacgtggc gacgaacgca tctgggatat
    13441 cttagaagaa gttatcaaag aacacccagt cttacttaac cgcgcaccga ctcttcacag
    13501 acttggtatt caggcttttg aacccgttct tattgacggt aaggcacttc gtcttcaccc
    13561 acttgtgtgt gaggcctaca atgccgactt cgatggagac caaatggcca ttcacgtgcc
    13621 actttcagaa gaagcacaag ctgaagctcg tcttttgatg cttgctgcag agcacatctt
    13681 gaaccctaaa gatggtaaac cagttgttac tccatctcag gatatggttc ttggtaacta
    13741 ctatcttacg atggaagatg ctggtcgcga aggcgaaggt atgattttca aggataaaga
    13801 cgaagctgtg atggcatatc gtaatggtta tgctcatctt catagtcgtg tgggtatcgc
    13861 tgttgacagc atgccaaaca aaccttggaa agacaatcaa agacataaaa tcatggtgac
    13921 aactgttggt aagattttgt ttaacgatat catgccagag gaccttcctt acctccaaga
    13981 gccaaacaat gccaacttga cagaaggaac agctgataaa tacttccttg aacctggtca
    14041 agatatccaa gaagtgattg acggcttaga aatcaatgtg ccattcaaga agaaaaacct
    14101 cggtaacatc attgcggaaa ccttcaaacg tttccgtaca acagaaacat cagccttcct
    14161 tgaccgtttg aaagaccttg gttactacca ttcaaccctt gctggtttga cagtgggtat
    14221 cgctgacatt cctgttattg ataataaagc tgaaatcatt gatgctgctc accatcgtgt
    14281 tgaagaaatt aacaaagcct tccgtcgtgg tttgatgaca gatgatgacc gttatgttgc
    14341 cgttacaaca acatggcgtg aagctaaaga agcccttgaa aaacgtctga ttgaaacaca
    14401 agatcctaag aacccaatcg ttatgatgat ggactcagga gctcgtggta acatctcaaa
    14461 cttctcacag cttgctggta tgcgtggttt gatggctgct cctaacggac gcatcatgga
    14521 acttcctatc ttgtcaaact tccgtgaagg tttgagcgtt ttggaaatgt tcttctcaac
    14581 ccacggtgca cgtaaaggga tgaccgatac ggcccttaaa acagccgact caggttacct
    14641 tactcgtcgt ttggttgacg ttgcccaaga tgttatcatt cgtgaggacg attgtggcac
    14701 tgatcgtggt cttcttatcc gtgctattac agatggtaaa gaagttaccg aaacgcttga
    14761 agagcgtctt caaggtcgtt acacacgtaa atcagtcaaa caccctgaaa ctggtgaagt
    14821 cttgattggt gctgaccaat taatcactga agacatggct cgtaagattg ttgatgcagg
    14881 tgttgaagaa gtgaccattc gttctgtctt tacctgtgcg actcgtcatg gtgtctgccg
    14941 tcactgttat ggtatcaact tggcaactgg tgatgctgtt gaagtgggtg aagcagttgg
    15001 tactattgct gcccaatcta tcggtgagcc tggtactcag cttaccatgc gtaccttcca
    15061 cacgggtggt gtagcctcaa ataccgatat cacccagggt cttcctcgta ttcaagagat
    15121 ctttgaagca cgtaatccta aaggggaagc ggtcattact gaagtgaaag ggaatgtcgt
    15181 cgagattgaa gaagatgcgt caactcgtac caagaaagtc tacgttcaag gtaaaactgg
    15241 catgggcgaa tatgtcgtac catttacagc acgtatgaaa gttgaagttg gcgacgaagt
    15301 taatcgcgga gctgccctta cagaagggtc aatccaaccg aaacgtctcc ttgaagtgcg
    15361 tgataccttg tcagttgaaa cgtaccttct tgcagaagta caaaaagttt accgtagcca
    15421 aggggtagaa atcggagaca aacacgttga ggtaatggtt cgccaaatgc ttcgtaaagt
    15481 tcgtgtcatg gatccaggtg atacagacct ccttccaggt acacttatgg atatttctga
    15541 tttcacagat gctaacaaag atattgttat ctctggtggt attcctgcga catctcgtcc
    15601 tgttcttatg ggtattacta aggcttccct tgaaaccaat tccttcttat cagctgcatc
    15661 cttccaagaa acaactcgtg ttcttacaga tgctgctatc cgtggtaaga aagaccactt
    15721 acttggtctt aaagaaaatg ttatcattgg taaaatcatt ccagctggta ctggtatggc
    15781 gcgctaccgt aacattgaac cacaagcgat gaatgagatt gaagtgattg atcatacaga
    15841 agtctcagca gaagccgttt ttacagcaga agcagaataa tctcatgaaa gagtccttag
    15901 gggctctttt ttgtctgagt ttgttggcag taaaaagcat tgacaatcag tcttagtccc
    15961 aatgagcaga tgtttttcaa aatacttatg atttagctat aataatgcat aagaggaata
    16021 ggtaatgtgt tatgtatcaa gtaattaaaa tgtatggtga ttgggagcct tggtggttca
    16081 tcgatggatg gcaagacgat attatagatg agcaacaatt tagtgactgg caagaagcgc
    16141 ttgattattt taatcaagaa tggcagcgca tgaaagctat ttttcctagt tatcatagtc
    16201 aaaagaattt gttagctact ttttgggaaa aagaagataa aagatggtgc gaggactgtg
    16261 acgaagattt acagcagttt cattctcttt tactccttaa aaataaagat attgtaccaa
    16321 gcaataatta tatccctgaa tttgaacaac gaaatgattc accacaggta gcttatcttt
    16381 gcaagttaaa cctttaagtg tttgttaaaa acaaaataag ttaaaagaaa atatcacttc
    16441 ttctgatatt ttcttttttt gtttacgaat aaatgagtaa ggaggaatta tggtacaagc
    16501 attagcaaaa gctattctag caaaagctga acaggttcag gcacaagata tttatatttt
    16561 gccaagagca gatcaatatg atcttttttt acgaatagga gatgaaagga gattagttga
    16621 tgtttatcag agcgatcgga tggctcctct tattagtcac tttaaattcg ttgcaggaat
    16681 gatagttggt gaaaagagac gttgtcaggt gggttcatgt gattataagc ttagtaaaga
    16741 taagcagtta tctttgcgct tatctagcgt gggtgattat cgcgggcatg aaagcttagt
    16801 gattcgtctg cttcatcatc aaaataaaag tgtacattat tggtttgatg gattgacaaa
    16861 agtagccaat caagttggcg gtagagggtt gtatttattt gcaggaccag ttgggtctgg
    16921 gaagacaacc ttgatgtacc agctgatttc gagttatcat caagaagcac aggttattag
    16981 tatagaagat cctgtagaaa ttaaaaatca ccagatttta caattacaag tgaatgatga
    17041 tattggtatg acttatgaca atttgatcaa actgtcttta cgccatcgac cagatatttt
    17101 agttattggt gagattcgag atagtcaaac agcaagagcc gttattaggg ctagtctaac
    17161 aggtgccatg gttttttcaa cggttcacgc taaaagtatc tcgggtgttt atgcaagatt
    17221 gttagaactt ggtgtaacga aagcagaact gtctaattgc ttagcattaa ttgcttacca
    17281 aaggttactt aatggaggag cattgattga ctctactcaa aacgaatttg aatattattc
    17341 ctcatcgaac tggaatcaac aaattgatca gcttcttgag gcaggacatc tcaatcccaa
    17401 gcaagctaag cttgaaaaaa ttatctagta aacatcagca taaattcatt caattactag
    17461 ctaatctttt atctacagga tttagcttca cagaggttat tgctttttta aaaagaagcc
    17521 agttgcttca gctagattat gttcttaaaa tggaagagtc tttattaaaa ggacaaggct
    17581 tagcagatat gttgtcgggt ttaggttttt cagatgctat tcttactcaa attagtttag
    17641 ctgatagaca cggtaatatt gaaacaacat tagtagcgat tcaacattac ttgaaccaaa
    17701 tggcaaggat cagacgaaaa actgttgaag ttatcaccta tcctcttatt cttttacttt
    17761 ttctttttgt aatgatgcta gggttgagac gctatttagt ccctcaatta gaaactcaaa
    17821 atcagataac ttactttctt aaccattttc ctgccttctt tataggtttt tgctcaggtc
    17881 ttattttgtt gttcgggatg gtatggctac gatggcgatc tcaaagccgc ttgaaactct
    17941 atagtcggct aagccgttat ccttttttag gtaaactgtt aaaacaatac ttaactagtt
    18001 attatgctag agaatggggt acgttaatag gtcaaggtct tgatttgatg gctattttag
    18061 acattatggc cattgagaaa tcctcactaa tgaaagaatt agctgaggac atcagaatga
    18121 gtctgcttga agggcaagcc tttcacatta aagtagctac ctaccctttt tttaaaaaag
    18181 aattaagttt aatgattgaa tacggtgaaa tcaaatcgaa actgggggct gagttggaaa
    18241 tctatgccca ggaaagctgg gaacaatttt ttagtcagct ttatcaagtg acacaactta
    18301 ttcaaccagc tattttttta gtagttgctg tgacaatcgt catgatttat gcggcaatcc
    18361 tattaccaat ttaccaaaat atgggaggta ttttttaaga tgattaatca atggaacaac
    18421 ttacgacaca agaagctaaa aggatttact cttctagaaa tgttattggt gattcttgtc
    18481 atcagtgttt tgatgctatt atttgtgcct aatttaagca agcaaaaaga cagggttaca
    18541 gaaacaggta atgccgctgt tgttaaatta gtggagaatc aagcagaact atatgaatta
    18601 tctcaaggct caaaaccaag tttgagccag ttaaaggcag atggtagtat cactgagaaa
    18661 caagaaaaag cttatcaaga ctattatgac aaacataaaa atgaaaaagc ccgtcttagc
    18721 aattaaagct tttactttgc ttgaaacttt actaagtttg tcagtgatga gtttcattat
    18781 attgggattg tctgtaccag tgacaaaaag ctatcaaaaa gtagaagagc atctgttttt
    18841 tagccatttt gaacatttgt atcgacatca gcaaaagtta gccatattac agcaaaaaca
    18901 gcgcgtcttg gatatttctt caacaaaaat tgttacagaa ggtaatagcc taacagttcc
    18961 taaatcaata acagtgaatc atccttatcg gttagttatt gatcagatgg gaggcaacca
    19021 ttccttagct aaaattattt ttgacatgac tgatcgacgc tttaaatacc aattttatct
    19081 agggagtggg aattatcaaa aaacaagtca aagcttacat agcccttgaa agtatgattg
    19141 caacaggcat cctctttagt attgtcattc tcgtcttaag cagtttacag cagagtcagg
    19201 ctgctctaac gtactatcga aagcagcaag aaaagcttaa tctagccttg atggcagtac
    19261 aaactagaac taaagagata acattgaatg gttgtcatat tactatttta cgtacggata
    19321 gatacattag tattcacgat gacgaaggag aagtgatgaa gattgagtaa acaattaagt
    19381 aacataaaag cttttactct tctagaggcg ttaatagcct tactcgtgat atcagggtct
    19441 ttattggttt atcaaggttt gacccaaacc ctccttaaac gtagccatta tctagcccgt
    19501 catgatcaag acaattggct cttattttct catcaattgc gagaggagtt aagtggagca
    19561 agattttaca aagtagctga taataaacta tacgttgaaa aaggaaagaa agtactagct
    19621 tttggccaat ttaaaagtca tgatttccga aaatcagcta gtaatggaaa agggtatcaa
    19681 cccatgttat ttggaatatc acgtaatcat attcacatag agcagtcaca gatttgcatt
    19741 actttaaagt ggaaaagtgg gttagaaagg actttttatt atgcctttca agactaatct
    19801 caaagcaggc atccttttgt atgctatgtt tatggctagt atttttttgc tggttttaca
    19861 ggtttacctt tctcaagtga cagctctaca caaggaatac caagctcaaa ctgactatgt
    19921 taaagcccga ctgatagcag aaatagttta tcaagatcat agatataaag cttctaatcc
    19981 tgtttctttt aaaggtggtc aagtcatttg tcgagagcga aaagagagat ggatgttaat
    20041 tgttaagtta gatcagcaac gacagtatca gtttgaatac cttaaaccca aacaatatag
    20101 tcaataaata gtattgttgt cataaatcct taaaatgatc caatatatag taattaaagg
    20161 tgacacttct tttcatttca agtgtaattt ggtatgataa ggttatgact tttgaaaaaa
    20221 ttgaagaagc ttatcagctg ctcttagaga actgtcaact aattgaaaat gatctaaaaa
    20281 cacatatata cgatgccatt gttgaacaaa actcctttta tttaggggct gagggagcta
    20341 gtcctcaagt tgctcaaaat agtgataaac tgaaagcctt gtgcctgaca aaagaagaat
    20401 ggcgtaaggc ctaccagttt ctttttatta aggcagctca gactgagcaa ctccaagcca
    20461 accatcagtt cacaccagat gctattggct tcattctgct gtatcttttg gaacaattga
    20521 gtgataaaga tagcttagag gtacttgaga ttggaagtgg aacagggaac ctagcccaaa
    20581 cccttctcaa caacacgagc aagagccttg attatgtagg gattgaactt gatgatctct
    20641 tgattgatct gtcagccagt attgctgaga taatggattc ttcagctcat tttattcaag
    20701 aagatgcggt aaggcctcaa ttactaaaag aaagtgacat tgtcatcagt gacttaccag
    20761 ttggttatta tcctaacgat gatattgcca aacggtacaa ggtggctagt tcagataagc
    20821 atacctatgc ccatcattta ttaatggaac agtctttaaa atacttgaaa aaagacggtt
    20881 ttgcgatttt tctggcacca gtcaatttat tgacgagccc tcagagccag ttattgaaac
    20941 agtggttaaa agattatgct caggtggtga ccttgattac gctaccagat tctatttttg
    21001 gtcatccctc aaatgccaag tccattattg tcttacaaaa acaaacagac cacccaatgg
    21061 aaacctttgt ctatccaatt cgggatttga agcttgcaga gaatattcat gattttatgg
    21121 aaaatttcaa aaaatggaaa ctgagtaatg tcaattaatt gattttttgt tataataaaa
    21181 aagcatcaca gaaaacgatt tcaagagagg ttaaaaatgt caaagacaat tgcaattaat
    21241 gcaggtagtt ctagtttaaa atggcaactt tatcagatgc cagaagaagc agtgttagca
    21301 caaggaatta ttgagcgtat cggtctcaag gattctattt caacagttaa gtacgatggc
    21361 aaaaaagaag agcaaattct tgatatccac gatcatacag aagctgttaa aatcttattg
    21421 aacgatttga ttcactttgg aatcattgca gcttatgatg aaattacagg agttggacac
    21481 cgtgttgttg ctggtggtga acttttcaaa gaatccgttg tggttaatga caaagttctt
    21541 gaacatattg aagaattatc agttttagct ccacttcaca atcctggagc ggcggcaggg
    21601 attcgtgcct tccgtgatat tttgccagat attaccagtg tttgtgtgtt tgacacgtca
    21661 tttcacacta gcatggcaaa acatacttac ctttacccaa ttcctcaaaa atattatacg
    21721 gactacaagg ttcgtaaata tggggcgcat gggacaagtc ataaatatgt agcgcaagaa
    21781 gctgctaaaa tgcttggtcg tccattagaa gaattaaaac taattactgc tcatattgga
    21841 aatggtgtct caattacagc taattaccac ggtaaatcag ttgatacctc aatgggattc
    21901 actccattag ctggtcctat gatgggaaca cgttctggtg acattgaccc agctatcatt
    21961 ccttatttaa ttgagcaaga cccagagtta aaagacgctg ctgacgttgt taatatgtta
    22021 aacaaaaaat caggtcttag tggggtgtca ggcatttcta gtgatatgcg tgatatcgag
    22081 gctggtttac aagaagataa tccagatgct gttttagctt acaatatctt tattgatcgc
    22141 attaaaaaat gcattggtca atattttgct gttttgaatg gagcagatgc tttggtcttt
    22201 acagctggta tgggtgaaaa tgcaccattg atgcgtcaag atgttattgg tggcttaaca
    22261 tggttcggaa tggacattga tcctgagaaa aatgtttttg gataccgtgg tgacatttca
    22321 actcctgagt caaaagtgaa ggtgcttgtt atctcaacag acgaagaatt gtgcatcgcg
    22381 cgtgacgttg aacgtttaaa aaatacaaaa taaaagagac taagtctcaa tagattaacc
    22441 aagtaaggta acactttact tggtttttta tgaggtttat ctgttggtta tggtgcctag
    22501 gtattagttc tgatcagtct ccttgctttt tgtaatcttg tgctaggcga tttggatgtc
    22561 agccttattt agaagttttt aattgcttca acgcttttct tgtatgtgtt tatagagccc
    22621 aatgatctaa aagactattt gataacggtt tatgatgagc gtaatcgtgc gattcgaaaa
    22681 cagacacaag tttactgctt ttacctgatg attgttctat ttgttgcctt attctttttc
    22741 ttcccttgct taaccatttg agaccttgtt tcttataagc tacctttgat ttttgcttac
    22801 ctttacttga ttatcagaag tctggtggct aagaaaatgg aggcactagg gaaaataaca
    22861 actaatagtc aaaaacctcc gtttgatacg agtcaggttc tttgttagat gacttgtcta
    22921 acggttgagt ttcctgtctg gtcaacggag gtgtttgtat tgttgtctga tttgtattgg
    22981 ggcacttatt ttacaagctt ttagccttat cgatggtttt gtcaatggca gagctgacag
    23041 tagttgtgag gccaaggcgt tctaactcca ttagaccagc aatagttgtg ccaccggggc
    23101 tacaaatagc gtcaatgaaa tcgtgcggac tttgagaact ggtcttgaga ttgctggcac
    23161 tagccaagac tgtttgagta acaatctcta gcgcctttgc tttaggcatg ccattcttga
    23221 cgccagcctt agccaaagcc tcaataaaga gataaatata ggcaggactt gaccctgcta
    23281 aagcggtaaa ggtgtcaaaa tccttttcac taatatcaaa tgtgctacca aagctatctg
    23341 ttaagtctcg aacacgtgct tgtaattcct gggacaccaa agcatttccc gttaaagcgg
    23401 tactgctttg gagaatttgt gcattcatgt ttggcatgat acgtagcagc ggaaggtctt
    23461 gtcctacgaa tgttgctagt cgttgaaggg aaatgcctgc tgccatagat ataataggct
    23521 gtttgaagtg aagcggtttg agtaccgttt caaatagttg aggcttgatg cctaaaataa
    23581 caagatcaac ctggtcaata aggtcttggt gggacatagc ataaggcagt gttaactgct
    23641 ccgcaatttc cttggaccgt tctaaagatg atcctgaaat gataagttca tggggtgttt
    23701 gtttaaggcc tttgatgata gcgctagcca ttttgccaac accaataatg ccaattttca
    23761 ttctgttgct ccttgtggga tattacggtt aatcaggcta acgctagttt agtaacgttt
    23821 aatcaagtca acggttgagc gatccagttt tttgataatg gcttgtaaga aggcttgggc
    23881 ttctacgaaa tcatccatag cgtagagggt ttgatgagag tgaatgtagc gtgcacagac
    23941 tccgatggtt gttgatggga caccaccatt ttgaaggtgt gcagcacctg catctgtgcc
    24001 tcccttgcca caatagtatt ggaaattgac accagcgtcc tcagcagtag tcagtaagaa
    24061 gtcgcgcata tctttgagca tgacatggcc tgggtcgtaa aagcgcaata aggtaccatc
    24121 tccgattgtt ccaggattgc cataaatatc accagcaggc gaacaatcta ctgcgaaaaa
    24181 gagttcaggg tcgaacttgg tggttgagac gtgggctccg cgcagaccaa cttcttcttg
    24241 aacgttagca cctgcaatta gggtgttgtt aaggtcttgt ccttttaacg cttcaagcat
    24301 ttctgttatc atgagaacgc catagcgatt gtcccaagct tttgaaataa tatttttttg
    24361 attggctgtt aggatcgttt cagattgagg gacaataata tcacccggtg taatgccaaa
    24421 gctttcagct tctgccttat ccgtaaagcc accatcaaac acaatatctt cgatatttgg
    24481 tagactagca gagccatttg ccccacgtaa aaaatgggga ggtaccgatc ctgaaataag
    24541 gggaataact tggccagtgc gtgtgtaaag ggtaaaccgt tgtgaactga caacaagtgg
    24601 gttccaacca ccgataccaa ccacgcgtag cgttccgtca actttgatat cactgaccat
    24661 aaaaccgact tcgtccatgt gcgcagctac taaaatacgg ggggcttttt cagctttact
    24721 atctctgata ccaaaaatgc caccaagccc gtctgtttca acacggtcaa ccagcggggt
    24781 tattttggtg cgtaggtagt cacgaacgct atgttcatag cccgcaatgc catccagttc
    24841 ggtaacttct ttgatttttg agaataagtc tgtcattttt catctccatt taaaaggttt
    24901 cagaccagtt caacgcggtc atctccttat attctaccac tttttaagta agaatgggtt
    24961 tcacagaaaa gaaaaatccc agagactaga gatctctgga agaagtgttt gtcttttggc
    25021 gataacctgt ttttttaggc agcaaggaaa tcattaaaat gataaagcac aagctggtta
    25081 ataagaatag tagggcgctt aggatgcgaa aagactcttc actagcgaaa agtaaggata
    25141 atggagtcat aaggagaacc ttccatttta ggttaatcag tgatttttat agcgtggtta
    25201 aattaataat aaaccacacc taatcaacgt acctttagtt tagttgtttt tactattttt
    25261 gtcaatacat aacagtagaa atctttagtt ttcttatttc agatcgtcca atttgatctt
    25321 tttatgataa aatgagggat atgagtaaaa agaaaatagg tatgatttca ggtatctttg
    25381 gatttagttt agctattggc ctaggaatag ttatcaaaga ttattgccaa gacaggcagc
    25441 gtcgacaaat gacaagggat ttacgcacct ttttttcacc tttaggacaa atcgaagttt
    25501 tatatatcaa tccttgtcag gtcaaacaag attatatttc aggtggggtt gttatgtcaa
    25561 acggcaaaca gtaccaattc acctatcaca gtcgacaaat tagttttgag gaggacaagg
    25621 gatgatcaga ccgacttctt atgagtcgtt agcggctctt attgaaaaag aagataagct
    25681 ggtgttattt tttacggcag actggtgtcc agactgtcaa tttatttatc ctattatgcc
    25741 agaaattgaa gcagaactta cagatatgac ttttgtttgt gttaatcgtg atcagtttat
    25801 agaggtagca caaaagtgga atatttttgg tattcctagt tttgtcgtga ttgaaaaagg
    25861 tcaggaagta gggcgtctgg ttaacaaaat gagaaagaca aaaactgaga ttatgcattt
    25921 tttagctgct tatcaataag aaaagaggaa gaataaatga tttttgcata caataaagaa
    25981 caagttggcg atgtcttgat ggttatctta caagacacca aagatatcaa acgtcaagta
    26041 gaacgaaaag gcaaagtagc ccgtgttttt gcagaagaaa gcggcaaaac ccttgcttgg
    26101 aatatctttg aagcgtcaag cttgattact attgaaggca atggacagat ttttttgaca
    26161 gacgaggacc ttgcaagatt aaatgcagag cttgctaagg aaggattttc agaaaggctt
    26221 gaaccgattg tgggacctgt ttttgtggtt ggtcaaattg ttgaaatggt ggctcatcca
    26281 gatagcgacc atcttaatat ctgtcaagtg gctattggtg aagatcaaac ggttcaaatc
    26341 gtagcgggcg caccaaatgc tgcgcttggt ttaaaaacga ttgttgcctt accaggtgct
    26401 ataatgccaa atggtagtct gattttccca ggaaaattgc gtggtgaaga aagttatggt
    26461 atgatgtgct ctcctcgtga gttagccttg ccaaatgcac cgcaaaaacg tggtattatt
    26521 gaacttgatg agtcagctgt ggtaggagaa gcttttgacc cagccaaaca ttggaaaggt
    26581 tagaaaaacc agctgaaata agactgtatt taactaaatg accagaaaaa aaggattagg
    26641 agcccttaat ccttttttct tatgttttca aaaacgttta aaatgaaact tattttacca
    26701 caatttttac gaataataag ataaggaggt cagtatgtat aataaagtga tagcaatcgg
    26761 tcgtttggta gctaaaccag aattggtaaa aacagctacg gataagcatg tagcacgtct
    26821 ctctttagct gttaatcgaa gatttaaaaa tgcttctgga gagcgagaag ctgattttat
    26881 ttcagttgtt gtttggggaa agttagcaga aactctggtt tcttatgcta gcaaaggtag
    26941 tttgatgtct attgatggcg aacttaggac ccgcaagtat gataaagatg ggcaagtgca
    27001 ttatgtgaca gaagttctct gccaatcatt tcaactgctt gaaagtcgtg ctcagcgcgc
    27061 tatgagagaa aataatgtta ctaatgatct agttgattta gtcttagaag aagatactct
    27121 tcccttttga tagaggctaa tgcttctcat cagggttcag ctgattactg aaatatatga
    27181 tgaggtgata tccgagcaac aaaagtgtga ctagatactt ctgataaaaa taaaaaaaca
    27241 ccttacgagt atgaacaaag tttcaagtgc tagatgttgg aactttgttc atactcgtaa
    27301 ggtgtttttt agtgtattac ttctttttgt tgctagtgtt aaggtttact tgcttacaag
    27361 cggttatcag gccactagaa ctcttataat aaatggagct ctttgagttt ggtgtcaatc
    27421 atttttaaga cagtcgccaa atcttgtggg ttctgaacaa aatcaaggga attgccatca
    27481 attttcattt ttggagagac ctcgtagtct tcgtaccagg ttgggtattc accgtggact
    27541 tgatggtaat attgctcaag actaggattg ccgtcgacct gctcaaaact tcggccacgt
    27601 ttttcaatgc gctcaagcat tttgtcaaaa gacacatcaa tgtagatgag aagatcaggg
    27661 cgttttttag gcatgccttc tagttcttct aacatgttgg cgagaagttc ctgatagatg
    27721 tcaagttctg tcttagtgac attgccattt ttgtagttaa gttttaagaa gagttcgtct
    27781 tcaaaaatag agcggtcaag aatgttgtta tcggcttgat aagcttcttt gatggactta
    27841 aaccgtttat tcaaaaaata aatttgaagt aaaaaggcgt actttttagg gtcttggtag
    27901 tagaggtcta gcacggggtt attatcaaca gcttcgtaaa aaacgtcagt tcctaaatgc
    27961 tctccaagtg cagcagcaag cgagctcttg ccagccccaa ttgttccagc tagtacaatc
    28021 aacatgcgtc tccttctaat gtcattagcg tactgtttcg aagatggctt caacctcagc
    28081 tagagtctca gcgcgtgaaa cagcaccacg aactttggca gcacctgctg ttccacgaag
    28141 atagtgtggc gctaagcccc gaaattcccg aacagcaatg gtttcacctt tgaggttaat
    28201 taggcgtttg aggtggtctt tggcgatgtc taattttttg gcaaagggaa gatctggcaa
    28261 ttcttgacca gtttcaaaga aatgattgat ttgggtaaaa agataaggat tgttcatggc
    28321 tgcgcggcca atcatcacgg catctacacc gatttcttca atcataaatt tggcgtcttg
    28381 caccgaacgc acgtccccat ttccaataaa gggaatctta gtgatagcct tagaaactcg
    28441 ggctaatgtc tcatggtcgc aggttcctgt atacatttgc tcacgggtac gaccgtgcat
    28501 ggcaagagca gatacacctg ccgactcagc ggcaagggca ttttcaacag caagtgaact
    28561 atccgcccaa cctgtacgca ttttgacagt cagtggaata tctaaaacag acgtgacttc
    28621 cttgacaatg tggtaaatct tatcagggtc acggagccac ttggcaccag cctcgttttt
    28681 gacaacttta ttaacagggc agcccatatt aatatcaaca atatcggcct tggtattggt
    28741 ttgaatgaaa tcagctgcac gtttcaaccc ttctgcatca ccaccaaata attgaatgga
    28801 catagggtgc tcattctcgt caatatggag catatggagg gtcttttcgt tgttgtagag
    28861 cagccctttt tcggaaatca tttccataac caccaaacca gcaccaaatt ctttggcgat
    28921 ggtacgaaag gcagaattgg tcacaccagc catgggagca aggacagtgc ggcgagggat
    28981 ttccacatca ccaatcctaa aggaagaatt aagctttatc attgatgatg gcctccaaat
    29041 cactttcatt aaattggtac ttggtaccac aaaattggca aacaatctca gcacccttat
    29101 cttcgtcaat catggcctgt aaatctgctt ttggcaaagt cattagggct gcttcaaaac
    29161 gttctctaga acagtcacac tgaaaactca gaggttcttc agaaagacgc ttgtaaggtt
    29221 catctccata gatggcctcc aaaagggcat ccacatgatt tttggaagct agtaggtaag
    29281 aaatggcagg catttcttgc aggcgttttt catagcgagc aatctcttct tcagaagctc
    29341 ctggaagcac ctgcaccata aagccaccag caaccttgac cttatcattt tcatctaaaa
    29401 gcacattaag tccgattgca gatggtgttt gttctgattc ggttaagtag taggcaaaat
    29461 cctcaccaat ttctccagta ataagaggcg ttgttgaggt gtaagggtta cctgtgccat
    29521 aatcaataat cgtcacaaaa tgaccattcc ccataaaagg cccaacaagg acttcacctg
    29581 ttgcggtctt tttaatatcg acaccagtat tttgaatgta acctttcaca tgtcctttgg
    29641 tatctgccac agagataatg tgtccaaaag aagaatcacc aatcactttg acggtgattt
    29701 tactatcgcc tttttgatta gccgccaaaa tctggttggc gatcaaggta cggccaagcg
    29761 caacagtaga cgatgacagc gtgttgtgtt tttcttgagc taatgcaacc gtctcagtgc
    29821 tatcaaggac gtaagcccta aaagcccctg attgtgctat tgatttaatt attttatcca
    29881 taacttagat tatagcataa agtgtaaaac ttgtattgac aggattactt gaaaaatcaa
    29941 tacagataga cagtaaaaaa tcccagagaa tgactcggga ttaagttaag gtgtggtcta
    30001 aagtcgcgca atgtggtgac atggcgctta tgttaattgc ttggttaaat cagcttggaa
    30061 tttttcctct ttaacttgat atatcaattc ttggatagat agaatcgatt catcaaaaga
    30121 tatttcagca acagcaattc cttttgtaag ctcatgttga acaaaaggaa tcagttggct
    30181 gtgagtgatg accaaatctg gctttaaatc aggaatttga taaacattat cttgcaataa
    30241 ataataggaa tgaaaattaa tgcttttatc cgagaaatac cttgggaaag aatctgttag
    30301 gagatgagca ttgataaaat tactggctac gaaaacaaca actaaaggag gttggatatt
    30361 tcttagaatt tgctcgacat agtggcaaaa aagatgaaaa tgcttatggt tcaagtaacg
    30421 cttaccagga agtttggcca tccactcttc gacaattaac tttaaggacg tatagagttt
    30481 ttggtttcct ttatagtacg gagaaatgaa taagttggtc tcaggaataa aatgttgcag
    30541 attaaacaag aatgattttg aaaaaaacat aagagctttt actaaactag ccttttgctc
    30601 ttttaggtta ggtaaaagag tgatgatagg atttaaaagc aggcgaaaag tatcattttc
    30661 ttcaaaaagt tgacaacatt gtctgatatg ctcaggtgtc cattgtaagc tcgcaaaaga
    30721 attattagcg gtgatataaa ttaaatagag gtagtccaaa tctcctgctg aaaagtttag
    30781 ttggcagtaa gtttcgataa tatcacggct actttttttc aaagaatcgt agacaaaaag
    30841 tttttttaat tgttgaaaaa ttctggtttg gggaatagtt accgaaaatt gatgccgctt
    30901 ccacgataaa gccaataaaa tgtcatagaa agaaaacgat tccgataacc aaggagaggt
    30961 tttaaggtgg gtggaactat gggataaaaa gctatgaata gtgtttttgt cttgctgcgt
    31021 caagtcataa actttaatgc caaacttact atatagcaga gcgatgaggt aacggatgcg
    31081 atattcctca ccgacaatct tgttcttaga gagttttaat tcaaagtttc ttaataaggg
    31141 aaccaatgct tcgcgcatcc gataagctga ggagtttgat aaaaaatgac ttctcgcaaa
    31201 atccgtaagg ggacgagagt gtgaaccatt ttttattaaa aaggctagta attgtaagac
    31261 attagatgat gcatagagtt ggtaaagata agtttcttta aaaggatgtg taaattggca
    31321 agatatcatc cttttttgga tggtcataga cagactacca gggaaaaagg cattcagttc
    31381 ctcacaataa tggtttagtt gtaaaaaggt taagccagtt ttttctgctg cctcagttat
    31441 tgacaaataa gatgtcttaa aaaacaagac aattaactga cattttgatt cgattgatga
    31501 ttccaagtat ttttctatca aggaaagggg tcctctcttc ctacacttag aaagccaaat
    31561 aagtctttga taagtgtatt ctcctatttc aattctatct aagctaaaaa tagaaatcaa
    31621 atctaattgt ttttggtgaa aagtcgaaac cttttgaaat agctttaaag caatattttc
    31681 tcaaaaaatc attttcaaaa acactaattt ggtgaaatat acaatcaaaa aaatatcttt
    31741 cttgacaata acatggtaag ctcatatatg tttgtgagag gagagaaaat gaataacaaa
    31801 atgtttttga acaaagaagc cggttttttg gcacacacaa aaagaaaaag gcgatttgct
    31861 gtcactttag tgggagtctt ttttatgctt ttggcatgcg cgggtgctat ccgttttggt
    31921 caagtagcct atgctgcgga tgagaggact gtgccgaatt ttaaaagccc agatccagat
    31981 tatccctggt atggctatga tgcttatact ggagcgtttt taagatatca tgatcttaga
    32041 gtcaatttaa acggaagtcg gagctaccaa gtttattgtt ttaatattaa aaaaaactat
    32101 cctcgacctt ttacgagtag tgataaaaaa tggtataaaa gattagaggg aactgctgaa
    32161 acttttaaag ttcatgctat ggctcccaga gtagggggag aggaactaac taaaaaatta
    32221 cggagcgtta tgtataacgg ctaccctaat gacggcaata atattatgaa aggtttggag
    32281 ccttcaaatg ctatagaagt aacacaatac gcagtttggt actattcaga taagtcacag
    32341 tatgaaattg agacattatg gaagtctgaa gcgaaagaag gtaagattag tgagtcacaa
    32401 gttactttaa tgcgtgaagc cctgaggaaa ctaatttcct ctgatgaaga tttggttaaa
    32461 caagttccat cgaactttaa acttagtact tttgagtctt ctgataagtc ctaccaaaat
    32521 cttttaagtg ctgagtttgt acctgatgat ccccctaaac ctggtgatac gtcagaacat
    32581 aatcctaaaa ctcccgagtt ggatggtagt cctattcccg aagacccaaa acatccagat
    32641 gataatttag aacctacatt gccgccagtg atgctagatg gtgaagaagt cccagaagtt
    32701 ccaagcgaga gcttagaacc tgcgcttccc ccattgatgc cagagctaga tggtgaagaa
    32761 gtccctgaaa aacctagtgt tgacttacct attgaagttc ctcgttatga gtttaacaat
    32821 aaagaccagt cacctctagc gggtgagtct ggtgagacgg agtatattac cgaagtctat
    32881 ggaaatcaac agaaccctgt tgatattgat aaaaaacttc cgaatgaaac aggtttttca
    32941 ggaaatatgg ttgagacaga aaatacgaaa gagccagaag tgttgatggg agg
//
LOCUS       DATURV010000013        31787 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DATURV010000013 DATURV010000000
VERSION     DATURV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31787
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.013"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..742)
                     /locus_tag="VCF75_000604"
     CDS             complement(<1..742)
                     /locus_tag="VCF75_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEP2546188.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQV"
     gene            complement(762..3248)
                     /gene="gyrA"
                     /locus_tag="VCF75_000605"
     CDS             complement(762..3248)
                     /gene="gyrA"
                     /locus_tag="VCF75_000605"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEP2546189.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELISTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIDEINTDRENSISKNKDN"
     gene            3439..4422
                     /locus_tag="VCF75_000606"
     CDS             3439..4422
                     /locus_tag="VCF75_000606"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEP2546190.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIIDKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            complement(4581..5951)
                     /locus_tag="VCF75_000607"
     CDS             complement(4581..5951)
                     /locus_tag="VCF75_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEP2546191.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIELFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDMSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(6192..7919)
                     /locus_tag="VCF75_000608"
     CDS             complement(6192..7919)
                     /locus_tag="VCF75_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546192.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVIIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQMVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DVGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRDEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            complement(7916..9640)
                     /locus_tag="VCF75_000609"
     CDS             complement(7916..9640)
                     /locus_tag="VCF75_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546193.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLISVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHIGYASLLSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLDPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KEQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEAPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPINDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKVLKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGEQK"
     gene            complement(9650..10249)
                     /locus_tag="VCF75_000610"
     CDS             complement(9650..10249)
                     /locus_tag="VCF75_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEP2546194.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIKHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            complement(10250..11227)
                     /locus_tag="VCF75_000611"
     CDS             complement(10250..11227)
                     /locus_tag="VCF75_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEP2546195.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVGQVKEVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            complement(11234..12490)
                     /locus_tag="VCF75_000612"
     CDS             complement(11234..12490)
                     /locus_tag="VCF75_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEP2546196.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAITDHVGAYLMVDMAHIAGLVAAGVHPSPV
                     PYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALVPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIARLIIKALVN
                     HNQTVILEEVRQEVRQLTDAFPLYKK"
     gene            complement(12480..12932)
                     /locus_tag="VCF75_000613"
     CDS             complement(12480..12932)
                     /locus_tag="VCF75_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2546197.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            complement(12950..13540)
                     /locus_tag="VCF75_000614"
     CDS             complement(12950..13540)
                     /locus_tag="VCF75_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEP2546198.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITAALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            complement(13524..14363)
                     /gene="prmC"
                     /locus_tag="VCF75_000615"
     CDS             complement(13524..14363)
                     /gene="prmC"
                     /locus_tag="VCF75_000615"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEP2546199.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFSEIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            complement(14363..15442)
                     /gene="prfA"
                     /locus_tag="VCF75_000616"
     CDS             complement(14363..15442)
                     /gene="prfA"
                     /locus_tag="VCF75_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEP2546200.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDLNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            complement(15477..16046)
                     /locus_tag="VCF75_000617"
     CDS             complement(15477..16046)
                     /locus_tag="VCF75_000617"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEP2546201.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            16184..16369
                     /locus_tag="VCF75_000618"
     CDS             16184..16369
                     /locus_tag="VCF75_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEP2546202.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            16422..17360
                     /locus_tag="VCF75_000619"
     CDS             16422..17360
                     /locus_tag="VCF75_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEP2546203.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPVSVHSDLFNLVQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKIFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTIMAPSSFYCDIWTTRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(17424..18707)
                     /locus_tag="VCF75_000620"
     CDS             complement(17424..18707)
                     /locus_tag="VCF75_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEP2546204.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIH
                     VPTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIVAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(18707..19288)
                     /locus_tag="VCF75_000621"
     CDS             complement(18707..19288)
                     /locus_tag="VCF75_000621"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEP2546205.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     regulatory      complement(19375..19470)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(19593..20576)
                     /gene="guaC"
                     /locus_tag="VCF75_000622"
     CDS             complement(19593..20576)
                     /gene="guaC"
                     /locus_tag="VCF75_000622"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEP2546206.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAGQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAYEY
                     EFVTSLKEDAPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVNGETFKEYYGSASAYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(20854..22380)
                     /locus_tag="VCF75_000623"
     CDS             complement(20854..22380)
                     /locus_tag="VCF75_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEP2546207.1"
                     /translation="MPSLFITFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGTAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITFL
                     VLLASYTPMVLSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAEEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKASLLTKHPELKGILNQLAGKITEKEMQDMNYKVSVKGADANKVARDYLLKAGLIQK
                     "
     gene            complement(22373..23101)
                     /locus_tag="VCF75_000624"
     CDS             complement(22373..23101)
                     /locus_tag="VCF75_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546208.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNAPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWAPEEVRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVANLFGGDEHA"
     gene            23521..24210
                     /locus_tag="VCF75_000625"
     CDS             23521..24210
                     /locus_tag="VCF75_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEP2546209.1"
                     /translation="MKNAISILALLTPENQLEETFIRELSHHFSHLFEVTILTSKANI
                     QSNQLSTFQGIFSFHEHDIDFPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTGF
                     YQKFDGHFLQYLPLQARLSDANGNIIVDNHAFNGSFLPTTDKEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            complement(24404..25159)
                     /locus_tag="VCF75_000626"
     CDS             complement(24404..25159)
                     /locus_tag="VCF75_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP2546210.1"
                     /translation="MGNIALVTGASAGFGRAIVEHLIADGIKVIGASRRLEKLKVLET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGFDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGSHIRVTNIEPGLCEGTEFSKVRFNGDEERVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(25286..26281)
                     /gene="pta"
                     /locus_tag="VCF75_000627"
     CDS             complement(25286..26281)
                     /gene="pta"
                     /locus_tag="VCF75_000627"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEP2546211.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKETFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            complement(26285..27190)
                     /locus_tag="VCF75_000628"
     CDS             complement(26285..27190)
                     /locus_tag="VCF75_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP2546212.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            complement(27187..28023)
                     /locus_tag="VCF75_000629"
     CDS             complement(27187..28023)
                     /locus_tag="VCF75_000629"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEP2546213.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEI
                     DS"
     gene            complement(27998..28669)
                     /locus_tag="VCF75_000630"
     CDS             complement(27998..28669)
                     /locus_tag="VCF75_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP2546214.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            28757..29335
                     /locus_tag="VCF75_000631"
     CDS             28757..29335
                     /locus_tag="VCF75_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEP2546215.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFVNSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHITFKYAKSKVARCINTLKKFNDK"
     gene            29475..30455
                     /locus_tag="VCF75_000632"
     CDS             29475..30455
                     /locus_tag="VCF75_000632"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP2546216.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAAEVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            30452..31579
                     /locus_tag="VCF75_000633"
     CDS             30452..31579
                     /locus_tag="VCF75_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP2546217.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     NTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            31569..>31787
                     /locus_tag="VCF75_000634"
     CDS             31569..>31787
                     /locus_tag="VCF75_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP2546218.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLETIPNSGDGFPLKITINKELTGFKLSI"
BASE COUNT         9810 a         6602 c         5531 g         9844 t
ORIGIN      
        1 atacttggtt ataagaatga ttaaaagctt tcaatacatc ggcgggcgct ttatcaaaat
       61 cgtattctgt ttttagttct cctttgacaa taatacgttc tgttgcttcg atatctgtac
      121 aagtcactaa agtcacttct ttgagaccag ctgtatcatc gataacatca acgcgttcag
      181 gagctaccgt gaaaacatct tttatgatgt attcgtaaat tttttcttta tctgttaaat
      241 agatggacat cccattttgt gctctttcaa gcggcgaaaa gagcatttga gatgaacctg
      301 taattccaaa aatatgatga ctggcaagag aataattatt ttctcctccc ataacttgtt
      361 cttctttcat cgttcctgcg ccataaataa gctcagtatt tcctaaacct ttaaaaattg
      421 gtaaattaat gccaagctct ggtatggcaa tgccccctat aacaggaagt tgctgagccg
      481 ccatttgtgc ttgcaagaca ctctctgtac taactggctc tacggcttgg aaatcaaatg
      541 ttgacttagc ctctttattc tttttgattt gttttttcga aactttcgtt acttgatatt
      601 tattagagtt tcgagcaatt aatgtatttc ggataggttt gttaaaaagc aatgctaaac
      661 ctagaattaa aagtacagct attagtagtt ttcttgccca agacatggat tttatttttc
      721 gacgcttttg tttttttacc attttttact ccttgttatg attaattatc tttatttttt
      781 gaaatactat tttcacgatc agtattgatt tcatcgatac tactgtcttc aggctgaacc
      841 aaggtaaatg ttacaatctt agcatcagca tctagtttca taattttaac acccaaagta
      901 gcacgacctg tttgcgaaat atttgcaaca tttgtgcgaa taatgacacc tttatttgta
      961 atcaccatga tatcttctgt accatctacg gtaaccaatc cagctaattg accattttta
     1021 ggggtaatat tagctgtttt tattcctttt ccccccctac cttttgtagg atagtctgta
     1081 gcacttgttc tcttaccaaa gccattttcg gtaattacaa gaacttcttg gttatcttga
     1141 atacgtgacg cgccaacaac gcgatcatct tcacgaagct taaccccgcg aacacccgta
     1201 gctgatcggc ccatgttgcg gatgcttgct tcattaaaac gaacagaata tccggaatga
     1261 gttccaatga taatgtcatc ctgaccgctt gtcaaaagga cgttaatcag ctgatctccc
     1321 tctttcaact taagagcacg gagaccgttt tgacggatat tattaaattc tgaaacttct
     1381 gtacgtttga caatgccttg tttcgttgta aagaagaagg atttacctgc tgtttcttcc
     1441 ttacgtgcgt tgataatagt ttgaatggtt tcaccatctt ctaatttcaa tagattaaca
     1501 attggaagac cttttgctgt acgaccatat tctggaattt cgtaagcttt caaccgatag
     1561 acacgcccaa aattcgtaaa gaagagcaaa gtatcatggg tacttgttga tatcaattca
     1621 cggacaaagt cgtcattgtt gaccccagtt ccttggacgc cacgtccacc acgcttctgg
     1681 gcacgaaatt catcttgagc aagacgcttg atataacctt tatttgacaa ggtaatcaaa
     1741 acatcttctt cttcaattag gtcttcatct tctagagaaa ggacttcacc gaccattaat
     1801 tctgtacggc gtgggttagc gtatttacgc ttgatctcat ccatttcttc tttgatgata
     1861 gtgataatac gttctggttt ggctaaaata tcagataagt cagcaatcaa ggccaacaaa
     1921 tcatcatatt ctgattgaat cttatcacgt tccaaaccag tcaaacgacg caaacgcata
     1981 tccaaaatag cttggctttg gcgttcagac aagtcgaaac gtgacatcaa ttctgtctga
     2041 gcaatgacat ctgtctcgct gttacgaata atagcaatca cttcgtctaa atgatcaagg
     2101 gcaatcagta agccttctaa aatatgagct ctggcttcag ctttatcttt atcaaatcgg
     2161 gtgcgacgta taataacttc tttttgatga ctaatgtaat tatcaatgat ttgtctgagt
     2221 gacaatattt taggcacacc attttcaata gccaacatat taaaactgaa attcgtctga
     2281 aggcttgtta atttaaagag gttatttaag ataactgtcg cggatgcctc tcgacgaatc
     2341 tcgataacaa aacgcactcc ttcacggcta gattcgtcac gaactgctgt aataccttct
     2401 agtctttttt cttgtgctaa acgaacaatg tgttcatgca ctttcgtttt attgacgcca
     2461 tagggaaatt cagtaaccac aatacgttcc cgacctgttt gagttgtttc aatttctgtt
     2521 cgagagcgta aaacgataga acctttacca gtatcataag ccctatgaat acctgagcgc
     2581 cccataacaa gggcaccagt cggaaagtca ggtccaggga tcacttccat caactctctt
     2641 gttgtacagt ctggatgttc cattaccatc ttgacagcat caatagactc tgccaaatta
     2701 tgaggcggaa tgttagttgc catcccaacg gcaatccctg tcgctccatt gactaataaa
     2761 ttaggaaagc gagctggtaa tactactggt tctctttcgc ttccatcgta gttatcttga
     2821 aaattaacgg tatttttatt gatatccctt aataattcaa gcgcaatttt actcatgcgc
     2881 gcttcagtat aacgttgagc ggcagcacca tcaccatcca tagaaccaaa attgccatgc
     2941 ccatcaacaa gcatatggcg ataactccac cactgagcca tacgaaccat agcttcataa
     3001 atagaagaat ccccatgcgg gtgatattta cccatgacat cacccgtaat acgagcagat
     3061 tttttatgcg gtttatcagg tgtcacaccg agttcattca tcccatagag aatacgacga
     3121 tgcacaggtt tcagaccatc acgcacatct ggcaaagctc ttgccacaat gacactcatg
     3181 gcatagtcaa tgaaactcgt tttcatttca cttgtcagat tgacgtcaat taaatttcga
     3241 tcttgcattt aaggaatgct ccttttctag tcaatactag atttcattat accataaatt
     3301 ccttattttt tcttattgga agcgttatca ggaaactatt ttcattattt ttctgaattc
     3361 aatgacaaac aattatcaat atgttaaaat aagaccgtat gagcttttgc tctaataaaa
     3421 ttaaggagat gtttagaaat gactgcaact aaacaacaca aaaaagtaat cttggtcggt
     3481 gatggtgccg tcggttcatc atacgctttt gcattggtaa cacaaaatat tgctcaagaa
     3541 cttggtatta ttgatatttt taaagaaaaa actcaaggag atgcagaaga tttaagccac
     3601 gctctagctt ttacatcacc taaaaaaatc tatgcagctg actactcaga ctgtcatgat
     3661 gctgatcttg ttgtcttaac agctggtgct cctcaaaaac caggtgaaac acgccttgac
     3721 cttgttgaaa aaaaccttcg tatcaataaa gaggttgtta cacaaatcgt tgcatctggt
     3781 ttcaaaggaa tctttcttgt tgctgctaac ccagttgacg ttttgactta ctcaacatgg
     3841 aaattctcag gtttccctaa agagcgtgtc attggttcag gtacatctct agattcagcc
     3901 cgtttccgtc aagctctggc tgctaaaatc ggtgttgatg cgcgttcggt tcacgcttac
     3961 atcatgggag aacacggtga ctctgaattt gctgtttggt cacacgctaa cgttgctggt
     4021 gtaggtcttt acgactggct ccaagctaat cgtgacattg atgagcaagg cctagttgat
     4081 cttttcattt ctgtacgtga tgctgcttac tcaatcatcg ataaaaaagg tgctactttc
     4141 tacggtattg cagttgccct agctcgtatt acaaaagcta ttcttgatga tgaaaatgct
     4201 gttcttccgc tttctgtttt ccaagaaggc caatacgaag gtgttgaaga ttgctatatt
     4261 ggtcaaccag ctatcgttgg agcgtatgga attgttcgtc cagtaaacat tccacttaac
     4321 gatgctgaat tgcaaaaaat gcaagcctct gcaaatcaat taaaagctat catcgatgaa
     4381 gctttcgcta aggaagaatt tgcttctgct gccaaaaact aatcacaaaa gagacttcag
     4441 tctctttttg ttttgcctga tattcctttt ccaacgtgct ataagaggat aaacataagt
     4501 ttagaacttt tcaggttgca acaattaaaa agcaaaaaag ccagtgctgc tgaactgctc
     4561 tggctttttc ctatcagata ctagtctttg gcaccaagtg ctgccattgt gatgtagttg
     4621 taaggtttgt tgaaatgtgg taagaagaaa atatctgtca aagctaattt ttcaatggtt
     4681 acgccttctt ggatagcaag tgagaacatg tgaattccca ttgacatatc ttcacgcgct
     4741 gccatttgag cacccaagat acgacgtgag tctttatcgt aaacaatctt aatggttact
     4801 gggaagttac cgtgttcgat aaattcaggt ttttggttat cagtatactc agtcactgca
     4861 gcatcaaaac caagtcgttt agctttttca agagtcaaac cagttgatac catgtgcaag
     4921 ccgtaaatgg aaataccgtt tgagccttgg acaccaattc cttcaaggtc tgtaccacaa
     4981 gcgttatgtg ctgctacaat tcctgtacgg acggcatttg aagctaaagc aatgtagttt
     5041 gtatcgcgag tagcattatc gtagatagtt gcacagtcac cgatagcata aacacctgga
     5101 atagaagttt cttggcgttt attgacaagg aaagcaccat tacggaaaag ctcaatctta
     5161 ccatttccaa gagttgtgtt tggacggaaa ccaacggcga ggataaccat atccacatcg
     5221 tactcatttt tgtcagtaat gatcttttca accttaccat taccagctac ttctttaact
     5281 gtttcaccaa aggctagttg aataccatgt tcttccatgt ttttagccat taagtcagtc
     5341 aaatcacggt cgtaataacc tgccaagcaa gtgtccacga catcaatcag aaccacttct
     5401 ttacctttac gttggaaagc ttcagctaac tcaacaccaa tgtaaccagc accaactacg
     5461 gctacacgtt taatgtcttt gttttcaagc tttgcaatca catcagctga gttttggtat
     5521 aacttcacaa attgaagatt ttcaagagtt gcttcaaatt caagtgatcc ttctttgatt
     5581 tcggcacctt taatcggtgg taagataggt tgtgagccag ttgcaaaaat caacttgtcg
     5641 taagtttcca cgtggttttt accatcaaca agcgctgtca ctgttttggc atcgtagtcg
     5701 attgattgaa caggtgattc catgtaaact ttagccccta aagactctaa ttcttcttta
     5761 tctgaataga aaagtccttc tggtccagca atttgctcac caatccaaag tgccatacca
     5821 cagcctaaaa atgaaatatt tgagttttgg tcaaatacaa caatctcatt agcatcaccg
     5881 tagtttgtta acatagtctt gatacaggcc gtaccagcat ggtttgcacc aacaacaacg
     5941 attttactca tatattttgt tcctcatttc acaatattat atagggctct attataccac
     6001 agtcaaaaaa cgcttccaaa tatattgctc acttttttga gttatttctt ctttagtcaa
     6061 ctgttttttc tcacttataa aaaaagatcg tccgcttttt cgctgttatt tttttatata
     6121 acagtttaaa gtttgttaat taactaactt taggaagagt taaaaagttc acctttgtta
     6181 aaagacgaac tttattctat cttttgtaat ctagcataac atccgttttt tgacattaag
     6241 ctggcatgtg ttccccactc aaccaatcga ccttgatcca taacaagaat caaatcagct
     6301 gattgaatcg ttgataaacg atgggcaatg ataaagctgg tccttcctct catgagttct
     6361 tttagtgcct cttgaataac agcttctgtt ctaatatcaa tagaggaggt ggcttcatct
     6421 aggatcaaga cttttggttt tttcaaaaac attctggcaa tagctaataa ctggagctgc
     6481 ccttgggaca aagcatcatc agaagcactt aagtaagtat cataagtctt aggaagttgc
     6541 ataataaaga agtgtgcatg agctgctttg gctgctgcca ctacttcatc acggctagcc
     6601 tcttcactgc cgtaagcaat caactcatga acagtcgcat ctttcaacca ggtttcttgc
     6661 aataccatgc cagtaatgga cctaagttct tccttagcat aacacttaat aggaacttta
     6721 tctaacttga tgctaccaac atcaagttca taaaagcgca tgagtaaatt gattaaagtc
     6781 gatttgcctg cacctgtagg ccctacaatc gctaccttgg aacccgcagg aacagaaaaa
     6841 ttaatatctt tcagtaaggg cctgtctttt tgataagaaa aacttacttc ttcaaagtca
     6901 atttgcccct taaccgctga tgccttaaat gttagaaagt gctcttgctc ttttatttca
     6961 atatctaaaa ggtcataaag cctttgcgca caagctaaag aactctgtat ctctgccaag
     7021 acagatgaaa tatcgttgaa aggtttggta tattggacaa caacatttaa aaaagtggtt
     7081 agctgaccta ctgaaaaaag tccagccatg atacgaacag cccctaaacc tgccagtaaa
     7141 gcataaatga cactattaat aaagcgagtg gctggattga ctgttgaggc gtaaaaaatg
     7201 gctttttgtg aagcctcgca ataggtttta tttaaaacat gataatcaca gatagactgc
     7261 tcttgagcat taaacagttg aaccaaacct tcttgacgaa ggatttcttc agtaaactga
     7321 gctaaatttc ctctgctttt ggtctgtgct tgtgcataat gaaaactctt ttgagcaata
     7381 aaacgagcta aaaagaggga gcttggtgtt aacaccaaaa ccaagattaa catgagccag
     7441 tcaatctgtg ccatagctat aatggtacac agaatcgtca atagtccaag tataaactgg
     7501 ttaaaaacca tttgtagacc attgatcagt tgctcagtgt cggttatcac tcgactaacc
     7561 aaatctccaa tggtttgacg atctagatat gcaaaaggaa gacgatgaat tttttccaaa
     7621 agcttatctt tcaattgctg gctatattga tatagcaaac gactatagac aagaggcatc
     7681 acccattgaa tcaatgtgtt gaccacaata ataactaaca tttgccccaa cagccatttt
     7741 aaagtttgcc aagaattgac tgacaaactc atatcaacgg ctttaccgat aagaacaggc
     7801 aagtaaacac ttaaaccaac ttgtacaaaa ctagcgatca cgagaataca aacaggtaga
     7861 ggttttttta acaaatcctg aagcactcgc tttaggaggt gatgatctgt tttaatcatt
     7921 tttgctcccc cttcacgtaa agcgctagca tttcttgata aagtgcattg cttactttta
     7981 actggtcatg actggcaaaa tccaattgat ggccctgttc caacactaaa atcttatcag
     8041 caaactgcaa attcttaatg gattgcgtga ctagaatcac taaaacatct gataactctt
     8101 cttttagaac tttaaaaagt cgtcctcgag tctcattgtc taaggcggaa ctggcatcat
     8161 ccaaaatcaa aatgggtttt ggcttcagaa gtgctctggc aatggctaga cgttgccgtt
     8221 gccctcctga aaaatgacgg ccgaaggctt caacaggtgc ctctaattgt tctggtaagg
     8281 cagcgacaaa ctcttttgct tgagccaatt caagagcccg ccacagctcc tcatcattaa
     8341 taggcattga ttgtcccagt aggaggttac ttctaatcgt tcctttaaat aattgagctt
     8401 tttgaggaac aagagcaatc acatttcgcc actcggttag tgtttttggc acttctcctt
     8461 gccaattaag actaatctca ccactatatt gtgaataagt ttggcagatc aagtcaatca
     8521 aagtcgtttt gcctgctcca gtaccaccaa tgatgccaat ccattctccc tgatcagcac
     8581 ttaactgaat atcatacaga cttggctctg ctgctcctgg ataagtaaag gtcaagtgcc
     8641 ggatggtcaa cagatgagtt gaaataggac catctttaag taagggcaaa ggcgcttcat
     8701 cagctaaaac aaacacctga ttaatccgtt ttgcggctgt tacactttga ttgatggttc
     8761 ccatcaacat ggtcattttc aaaagttctg ccaaaatctg caagaggtaa ttaattaaag
     8821 caaccaacat gccctgacta agcgatctat gagctacttg ccaacttcct tgccaaatca
     8881 agattaacag agtaagattg accactaaaa aagtcatagg attcaagaca ttggctaggg
     8941 tagcagcttg atattggtgg cgacttaaga gcatattttg ctccttaaat gcttggagtt
     9001 ctttttgggt ttgattaaaa gccttaatta ctcggattcc ttgtaactgt tggctagtca
     9061 aacgaaccaa gtgatctgtt tctcggcgga ttaagagata aaggggatct aataaatgtg
     9121 acataacaaa aacaagtgtt agtaagacaa tcaccatcat cacaaaccat agggttagac
     9181 ttggactaat ccaataagcc attaccatag caccacatac gataataggg gcccttagaa
     9241 aaagacgcaa aaattgattg ataccagttt gaatttgaaa actatcactg gtcaatcgtg
     9301 ataacagact agcataacca atatggtctt ggtcctcctt gctcaaaaac atgatttttt
     9361 taaacaaatc atctgtcatt tgtcttgtaa aaccaacagc tgctttcgaa gaaaaatact
     9421 gggctgtaac ggaaatcaat aagccaatgg ttgctaaacc aaataatgtt aaaacaaccc
     9481 tcaaaattcc ctggctgttg tgttgactaa tcgatatatc aatcatgtta gcaattaaca
     9541 aagggactaa taattcaaat aaagcttcta ataatttaaa aagaggacct aagatacttt
     9601 cttttaggta ccctttcaaa tatggacgta atcgtttcat gataactttc taaaaaaggc
     9661 tcatcagttc aattaaataa agattgaaat gaacttctcg tgagtaatag atattgccgc
     9721 cattcttata aagcttacct ccagaaatca aggcgagggg gtggtaataa aaataggttt
     9781 gaccactagt gttgcctagg cttggcgcca caactgtttt ggaataggta gtcgctaaat
     9841 caacggtatt ttgacctcca tgtttagcaa tataatcaat ataagctgtt ccaaaattat
     9901 aggcctgaac agccgtccaa gaatctactc ctgcttcctc agctagagca agattatgag
     9961 acagtaaatt caccccgtgc ttaatactgg cctgactatc tgtaatcgag tttttttgtc
    10021 ctgaactgct ttcactggat tgcatgacat ctgcttctcc tccttttgtc tcggtgtata
    10081 tcatagctaa gactaagtct acatttgcct ttgtatcatt ttctgctaat gttttctcaa
    10141 ccataggctt atatgccaaa acacgttgaa cattgtgatg aataacaaag gattgataaa
    10201 tgacaaaaag aagaagaaaa ctgcaggcac gctttaaaag tctaaacatc tatttggttt
    10261 taatatcctc tatattttta atcaagattg aataagtccc atcatcattt aacaaaaatt
    10321 caaccgcttc agcatcctga taaatggcat ttggtactgt taattctatc ccgtttgata
    10381 acgatagctt ttgattttct agttttttta tctgtctaga atgatcaata tcgctaattt
    10441 ttataggttc tggaatgact tctttgactt gacccacaaa ggttaaacgg gctgttaaat
    10501 tatcatcaaa gagttggtca gctaattttt ctggtgataa aacgtcgtct gcttctaact
    10561 gcttaaaaag agtggatttc atctttgact gaaaagtaaa atcatcttga ttaaaatgct
    10621 ctgcaatttt ttgagcggtt tgttctatca ttttgataga ctttttaaca gattgctcag
    10681 gtgtaacttt caagaggtgt tctgaaaaat aattagcaaa actgccatta tgcttcacac
    10741 gcttttcaat caagtagtac tgaccagatt tgagattaat gactagtgct tcatctggtg
    10801 tctgggtggg actcggcaga ttattctgcg tcaccgtaaa tggatgttca taattgtcag
    10861 aaaggtgagc aaactgttct ttcaaagcaa ttcgcaagaa agcaaaaaag ggttcgccat
    10921 ccttatcaaa ctggacaaag actaaatcat tggttttttg atcctctgaa ataacaaagg
    10981 cttccttcca taactgggca atggtaacag acgtttccaa caaatcatca ccaatggttg
    11041 taaaaaaggt attattagcc tcaaactgac cacgtttggc ttcgtccgag aacactttgg
    11101 ccaatttttt ccggaaatat tcatcaattc gcggtgtaat gctcactaaa cgatctgata
    11161 acagtaactc agtgtcattg ggagaaaatt gatgaatgac aatacgtttt atataactat
    11221 ctaacatgat tccttacttt ttatagagtg ggaaagcatc ggttaactga cgcacttcct
    11281 gacggacttc ttcaagaata acagtctgat tatgattaac caaggctttg atgataagac
    11341 gggcaatggt ctgactctct ttaacaccca taccacggct ggttatggca gcacaaccaa
    11401 tacgaatgcc tgaggtttta aatggcgaca aggtttcaaa aggaatggca tttttattaa
    11461 gggtaatatt aacctcatcc agaagatttt gcgctagttt accattagca ataaccttag
    11521 tgacatctac taaaaagaca tgattatctg taccacctga gatcagacga aaacggtcat
    11581 cttgtgcaaa aactgctgcc atagccgcag tattatcaat aatagcttgt gcatagtctt
    11641 tgaaggctgg aaccaaggct tctttgaagg caaccgcttt ggcagcaatc acgtgttcca
    11701 gaggtcctcc ttgtaaacca gggaaaacgg ccgaattaat ctttttagca agagcttcgt
    11761 cattggttaa tatcaagcct ccacgaggac cacgtaaggt tttatgggtt gttgacgtca
    11821 caatatgcgc ataagggaca ggactcgggt gcacacctgc tgctacaagg ccagcgatgt
    11881 gagccatgtc caccattaag taagcaccta cgtgatcagt aatggcacga aacttttcaa
    11941 aatcaatgct tctagagtag gccgaagccc ctgcaacgat aagctttggc tggaccgcct
    12001 ttgcttgttc taaaatcgcc tcataattta acatttcagt atcagcatct actgagtaac
    12061 ccacaaaatg gtaggttttt ccagaaaaat ttactggaga gccatgcgtg agatgaccac
    12121 ctgcagccaa atccattcct aatacggtat caccagcctc aattaaggcc atataagctg
    12181 ctgcattggc ttgacttccg gaatgggctt gaacattggc aaatgcggca ccgaaaagtt
    12241 tcttagcacg ttcaatagca agcgtttcga caatatccac acattctgtt ccaccataat
    12301 agcgattacc tggatagcct tctgcatact tattggttaa aacagaacct tgagctgcca
    12361 tgacagcttt tgaaaccatg ttttcagatg caatcaattc gatatgatgt tcttgtcttt
    12421 cttcttcagc atgaatggca tcccaaagct ctttatcaaa gtcttcaaca ttacctttat
    12481 caaaaatcat tgtcatctcc ttgtaagttt ttgatgaatc gtaattgtgt tttatcatct
    12541 gcatagtcga ggtttcggta aaaagcatgc gcttctttac gatgactggc agaattgagg
    12601 cgaataaagg caatgccttc ctgcctagct tgggattcca gggcccttag taaggcactc
    12661 ccaattcccc ttctctgata ggctggtaaa acagccaagc ccaagagatt aagaccatca
    12721 gaagcataaa gggtctcata ccgttcagcg tgaacatagc ctaataattg attggtatcc
    12781 ttgtcggcat aagctaaaaa atagtgatgg caatcctcaa tcaatcgttc atattgcctt
    12841 tctaacaagt caagcgaaac taaatagcca agggctttgg cgttaatttc ttgtagcata
    12901 ggacagtccg ttttacttaa tggtctcaac attccaagcg gctcctctct taaaagcgaa
    12961 gttcaggcac gttggctaat aattcttctt tggtgatagc accttgacgt aaaatcacag
    13021 cacgctcacc tgataagtct aagatggttg aatccttacc tgttagaaaa ggatcatctg
    13081 cataaccaaa cacttgaaag tcaaaatctt gcataatatg atcaaaaact cgaccactag
    13141 ctttgccaga taagtttgca gaaggaccaa ttaaagggcc tgttttttga attaaagctg
    13201 cggtaattgg atgactgggt aagcgaaaac caaccgttga aagacccgaa ttaatccaat
    13261 aaggaacctg gtcattagct tttaaaataa tcgttaacgg acctggtaaa aaggcttggt
    13321 ataatttctt gaggtatctc ggctgttctt ttgaaaaggc taagatacta ttaaaatcag
    13381 ccacattaag gttcattgct ttatctcggg gtctttgttt taagtcataa acagcattta
    13441 ctgctttctc atctaaagcc ttggcaaaaa gaccataaac cgtttctgtt ggcaaaacaa
    13501 gggcatcacc tgattcaatg atactagcta gatactccat aatcaacaac caccatcctt
    13561 tctttgccaa atatatccgt tacgactcgt atatgtcttt gaggaaaata ggcttgtaac
    13621 atatctttta taccttcagc ttgcttataa ccaatttcaa agtaaagctt cccctcctta
    13681 gttaagtaat tgtcagcttg ttcaataatc ttacggtaaa tagcatagcc attttcttta
    13741 gcaaaaagtg ccaaatgagg ctctgattgc aaaacattta gactgacttc ttccttatcc
    13801 tcgtaggata tgtagggagg atttgacaca ataatatcaa aggttcctga aatgagacta
    13861 aaaacatctg attcaataaa cgttatatca agctggtaag catcagcatt ggcttttgcc
    13921 aagtcaagag cagctctaga aatatcagat gccgttactt gccagttagg cctttctttt
    13981 ttgagagaaa tagcaatcgc tccactacct gtaccaatat ctaaaacatt caacggtgca
    14041 tcaagatttt cagctaaaat catgtcaact aattcttctg tttctggcct tggaattaat
    14101 acacgcttgt cgactgcaag cttcaaatca cgaaaataag cattacctgt aatatattga
    14161 ggagataaat gctgacttag agaacagaaa atatgttcca aaagaacagc atcttcagga
    14221 gtcacagctt gattttggtg aatcaacata tctagacttg accattcttt gatttcgcta
    14281 aaaacatagg cgagattttc gcgatcctca tctatttgtt ctagtttatc ttcataagtt
    14341 ctgattaatg tagcatagtt cattagttat tgagagattc taatttcttt gtctgatcat
    14401 acataacgag tgcatcaata acttcatcca tttttccaga taaaatagta tctaattttt
    14461 gaagtgtcaa accaatacga tgatctgtta cacgattttg tgggaaatta taagtacgaa
    14521 tacgttctga acgatcacca gtaccaactg ttgatttacg ttcagcatct tgttcatctt
    14581 gagcaatttg cgcaaaatgg tcagcaacac gagcacgaat gattttcatc gctttatcac
    14641 ggtttttttg ctgagtacgt tcttcttgca tttcaacttt gattcctgta ggaatgtgta
    14701 ccatacgaac tgcagtagca actttattga cattctgacc accagcacca gacgcgtggt
    14761 aaatatcgac gcgaaggtct ttaggatcaa tgtcgtattc tacttcttca acttctggca
    14821 ttaccaaaac agttgcagtt gacgtatgaa cacgaccctg actctctgtc accgggacac
    14881 gttgaacacg atgagctcct gattcatatt taagcttaga ataaactgac tgaccagaaa
    14941 ccatggcaac cacttcttta ataccaccaa caccattaac ggatgattcc ataacttcaa
    15001 aacgccatcc ttgagtttca gcatattttt gatacattgt taaaagatca ccagcaaaaa
    15061 gcgctgcttc atcgccacca gcagcaccac gaatttctaa aataatgttt ttatcatcgt
    15121 taagatcttt aggtaataga aggattttta atttttcttc atactcttct ttagctgctt
    15181 ttgattcttt aagttcttct ttagccattt cttctaattc aggatcacca ctggcatctt
    15241 tgatcatttc ttcagcatct gaaatggttt gaatgacttg cttatattct ctataagctg
    15301 tgacagtttc tcgagtgtta gcttcttcac gtgataattc cataaatcgc tttgtatctg
    15361 aaacaacatc tggatcacta agtaactcac ctaactcttc ataacgatct tcaactgcct
    15421 gcaattgatc ataaatattc atttttcttt gtatctcctt taaaaccgat taccttttaa
    15481 atctctggat taaaataatg ttttcgacaa actgggatat atgtttcatt accaccaatt
    15541 tgtatctgat caccttcata gacaggtttt ccgttttctg tccttaaaac catggttgct
    15601 ttcttactac agtattggca aattgttttg atttcatcaa tcttatcggc caataatagc
    15661 aaatgttttg aaccttcaaa aagattgttt tggaaatcat ttttcaaacc aaaagccatg
    15721 acaggaacat ttagctcatc caccacacgc gccaaatcat aaacattttg cttgcttaaa
    15781 aattgacttt cgtcaatcag gacacaataa ggtttttctt ccaattgggc aataaatgtg
    15841 aaaatatcca tgtcatttga aatgggaata gcttctcgtc gcataccaat acggcttgag
    15901 acgatgccaa aaccatcacg tgtatcaaga gcacttgtca taatgacaac aggtttgcct
    15961 tgttcttcat agttatgagc aacttttaaa atttcaatgg ttttaccaga attcattgtc
    16021 ccatatttat aatagagttg agccaaagcg gaaaatccta ctttctatag tattctacct
    16081 attttaccac aaaggctgaa gttttcccag ccattgaaaa cagtgtgtac tttttcatag
    16141 cattatgtta caatgatgcc caataatata aaggagtaac attatgccat ttgtaacaat
    16201 tgacttgttt gagggacgct cacaagaaca aaaaaatcaa ttagctcgcg aggttaccga
    16261 agtagtatca cgcattgcaa aagcacctaa agaaaatatc catgttttta tcaacgatat
    16321 gcctgaagga acttactatc ctcaaggtga aatgaaacaa aaatcataat gaccttcccc
    16381 cactccttag tgggggtatt ctgctaaaaa gaggactaat tatgtttgtt acgcaacaat
    16441 taaagctaat gggaacagtt attgatattc agatcgaatc tgacaaagct tgccaacaac
    16501 taagtagagt cattgactta ttatatactt acaaaaatcg tttttcagct aatgattcaa
    16561 actctgaatt gatggctatt aatcaggcgg ctggtgttaa acccgtcagc gtccactctg
    16621 atttatttaa cctagtccaa attggtaaag ctcatagctt gtcaacacca agtaatctta
    16681 atatcgccat tgggccactt gttcaagcct ggcgaattgg atttgaagat gctagagtgc
    16741 ctagtcataa ccttatctca caacagttag ctctaacaga tcctagacag gttcttatag
    16801 atgacaaaaa acagactgtc tttttacaac aagtaggaat ggctttagac ttaggagcct
    16861 tagcaaaagg ttatattact gataagatta tggcctacct gattgaagat ggtattgact
    16921 ctgcccttat taatttaggt ggaaatgtac gcgttcatgg acctaatccg aaaagccctg
    16981 acaagatttt tcgaattggc attcaaaaac cagatgctaa acgtgggcaa catcttggtg
    17041 tcatcaaggt taacaatcac tctgttgtga cttctggaat ttatgaaagg cagtttacag
    17101 ccaaagggaa acagtatcat catatcttag atcgtcagac aggctaccct atagaaacag
    17161 atatgcttag cttaacgatc atggctcctt cttcttttta ttgtgacata tggactaccc
    17221 gtttatttgg tcttgatagc tcaatgatta tcacactttt aaataccttt gataatattg
    17281 aaggtcttct agtaacaaga aagcaccatg tccttatgtc aaatggtttg agacactatt
    17341 ttcaaccata ctatcattaa aaggggttaa cggtttgctc aaaactgatc acagaaaaaa
    17401 cgactcaatt agccgttttt taattattca tcttgtttgt ctttgccatt taaaactaaa
    17461 tttaagacaa cagatgtcaa agtggcaatc acaataccat tcgttaagaa catttgagct
    17521 gtctctggta agctggcaaa aagattagtg ccattaaagc ctaaacctgc tgagatggaa
    17581 acggcaacaa taataaaatt atactcgttc ttttggaaat caacacgatt aagcatttgc
    17641 attccttgaa gagcaaccat accaaagaga acaagcatgg ctccaccaag aactgggctt
    17701 ggaatcattt gtgccattgc tccaaattta gggagtagac caatgaccac aaggatacct
    17761 gctgcgtagt aaatcggacg acgggtttta atacctgaga tttggacaag tccaacgttt
    17821 tgagaaaaac cagtgtaagg gaaggtatta aataacccac cgagaaagac tgcgatccct
    17881 tctgagcgat aaccattgcg caaacgtttt tcatctaatt gatcattagt caaatctgaa
    17941 agcgctaagt agacacctgt tgattcaacc atagaaaccg ttgcgatgat acacatcatc
    18001 acaatagaag tgatttcaaa agttggcatg ccaaaataaa atggggttgg cacatgaatc
    18061 caagaagcct ctacaacagg agttgtatct actaatccca tcatggctga aaccaaagtt
    18121 cccgctacaa gcccaatcaa aatggaaatg gatttcacaa aacctttggt aaatttttgg
    18181 actaaaagaa taatgacaat cgtcaacaat gatagcatca tgctttgtgc cgtaggctct
    18241 ttgacgttat ctcccatatt ccccatagca acacctacga gacttaagcc aataacggta
    18301 atgaccgatc ctgtaacaat tggtgggaag aaacgagcaa tcttagaaaa aatacctgcc
    18361 actaaaatga cataaattcc tgacgcaatg agggctccaa acatggcacc tgaaccttgc
    18421 tgcgctccaa taatggatag aggtgccact gattgaaaag cacatcctaa aacaactggt
    18481 aatcccactc ccgtatgctt ggttaacttt aactgtaaga aagtagccac accacacatg
    18541 aaaatatctg tcgaaatcag gtaagtcaac tctctagcgg aataacctaa agcacctgca
    18601 atcataattg gtactaaaat agatccggca tacatcgaaa gcacatgctg taagccaagg
    18661 acagctgatt gcgagtgaga atgttcctgc tttgttgaat gtgtcattat gcgtcagcct
    18721 ccaagaagtt tagattacca ttttcaaagt ttttaatacg ggctaatgag gtcacttcaa
    18781 tgcccatatc ttcaatcaat cgacgaccat cttggaaaga tttctcaatc acaataccaa
    18841 cgccgacgac ttgtgcccct gcttgaccaa taatctcgat caagcctttg gctgcctgac
    18901 cattagctaa aaagtcatca ataatcaaaa ccttgtcttc tttagatagg aatttaccag
    18961 cgatagacac cgtgctcgta acttgtttag tgaaagaata aacttctgct gtcaaaatgc
    19021 cttctgtcat ggtaatgttt ttatgttttt tcgcaaaaat cataggaaca tccattgctt
    19081 ctgcagcgta tacggctggt gcaatacctg aagcttcgat tgtaaccact tttgtaatgc
    19141 cagcctcagc atatttttga gcaaacactt taccaattgc tttcatcaac cggtaatcaa
    19201 cttgatgagt taaaaaatta tctaccttta gaatattctc tcctagaata ttgccatctg
    19261 ttaagatgcg ttcttcaagt aactgcataa atctcctttt gattcaaata gaaaaagcct
    19321 gccgcaaagc cagaaactat aagagtttct cactttgcaa ggttaaaaac caagcaagcc
    19381 taaaagctta cttattgtta gacattaagg tgtcttgtag agacgctgcg ccaattcaca
    19441 gcataaataa gtatgtcatt attgaattca ttatgataat ttactatttt accatgtttc
    19501 ttattttttt caatacctac agagatatga taaaagcatc tccagcatta agctaaaaaa
    19561 ttctttactg gtcctagcta actccatatc atttaaatgg aatcaccatt ccaaatagaa
    19621 tttttgacga taacataatc aacatgacgc aaactgtcta aatctttacc acccgcgtaa
    19681 gaaatggacg attgcaaatc ctgttgcatt tcggtcaggg tatctgataa gtgacctttt
    19741 gtaggcaata agattttttt gccttctaca tttttatgct cgcctttttg atatgctgac
    19801 gccgagccat aatattcttt aaacgtttca ccattcactt ctactgtttt tccaggactt
    19861 tcaatatgcc ctgcaaaaag cgatccaatc atgaccatac tagctccaaa gcggatggat
    19921 ttagcgatat caccatgtgt gcgaatccca ccatcagcaa tgataggctt acgagctgcc
    19981 tttgcgcacc agcgcagtgc tgctaactgc caaccacctg taccgaaacc tgtttttact
    20041 ttagtgatac atactttgcc tggaccgatg cctactttag tagcatcagc acctgcgttt
    20101 tcgagttctc gtacagcttc tggcgtacca acatttccag caatgacaaa tgtttctggt
    20161 aattcagttt tgatatgctt aatcatatca ataacactgt tagcatgacc atgagcaata
    20221 tcaatggtaa taaactcagg agcatcctct ttcaaagaag tcacaaattc atattcgtaa
    20281 gctttaaccc caacagagat tgatgcaatc aagccttgtt catgcatgcg cttgataaaa
    20341 ggtttgcggc tatcttcatc aaaacgatgc ataatataaa aataaccctc ttttgccaac
    20401 tgcccagcaa ttgtttcatc aataatggtt tgcatatttg ctgggataac tggtagcttg
    20461 aactggtatt ttccgagtgt cacacttgta tctgcttgtg aacgactagt aataatgcac
    20521 ttgttaggaa tcagttgaat atcttcataa tcaaatacag ggatgtcatt aaacataagt
    20581 ttctcctctt tagacacaga tgaaagaaaa taagtcagtt ctcatcttct tctccacgtc
    20641 ctttatacaa aatgaccttc ctatggcctc tgatgattag aggcatcgct aggtaaaaac
    20701 ttacctttcc ttttatagtt tatttcattg tttatttttt gtcaaattta aatactattt
    20761 ttaatttaaa tgttcggttt ttgtatttat gaattgttac ttcttgaacg taagagacga
    20821 aaaaatgcca gtcctacgga ccaacatttc tgtttatttc tggattaatc ccgctttcaa
    20881 cagataatct cgagcgacct tattagcatc tgctccttta acagatacct tgtagttcat
    20941 gtcctgcatc tctttttcag taatttttcc cgctaattga ttaagaatgc cttttaattc
    21001 tggatgtttt gttaataagg aggctttcat caaaggagct ccctgataag gtggaaacaa
    21061 ctgtttatca tcttttaaaa ctttcaaatg gtatttcgta atttcagcat ccgtagagta
    21121 agcatctgtt acttggatat ctcctgattg aattgcttga taacgaagag ctggctccat
    21181 cgtcgccaca gataactgta acccatattg agattgcatt cccttatagc catctgctct
    21241 gtccttaaat tccagagtaa aaccagcctt taaagtatca gcatgcgctt ttaaatcaga
    21301 aatggtttca atctgatatt cctctgccaa tttttctggc atagcaacag catacgtatt
    21361 ttggtaagca aacggcttaa gaagggtcag tttatcttgc ttagcaatgc cttttttagc
    21421 atcctcatag acctgcttag ggtcattaga caaaggtggt ttgtcacgta aaagacttga
    21481 tgttatggtt cctgtaaatt caggatagat atcaatatct ccagatttaa gagcttcata
    21541 taagaagcta gttttcccaa aattagattt caactcaacg cttatatctg attggtcttc
    21601 aattaattcc ttatagagat taatcagaat atcgggctct gctcccaatt taccggcaat
    21661 aaccactgta tcttttcctt ttgaaaactg actaagcacc ataggagtat atgatgccaa
    21721 caagacaaga aaggtgattc caaaactaat cataattcgt cgcaaggaag ctttctcaag
    21781 gtactgtaac aaactattga aaataatagc tagcagagct gaagaaatag cccctatcag
    21841 aatcaggtta gcattattac gatcaatccc caataagatg aaagagccaa gtccaccagc
    21901 tcctatcaag gaagctaaag tagctgtgcc aataatcatg actgctgacg tccgcacacc
    21961 tgacataatc acaggcatgg caattggaat ctcaaatgtt ttcaatcgct cccatttggt
    22021 catcccaaac gctgtccctg cttccacgag acttgggtca ataccattta accctgtgat
    22081 ggtgttctgt aaaatcggaa aaatcgcata gataactaaa gctgtcactg caggcaacgt
    22141 tccaattccc attaaaggga tgaaaagacc aagcaaagcc agtgagggaa tggtttgaaa
    22201 aacacctgtt acctgtaaca taatgtctga ccaacgtttg ctgcgactga gaagggctgc
    22261 taaaggcaca cctattagca aggcgatcat aagagataaa agggaaattt gcaggtgttc
    22321 ccccagagcc gccagccatt cattaaaacg gttttgaaag gtgataaaca aactaggcat
    22381 gctcatcacc tccaaataaa ttggccacaa aagcattagc agggtgggtc ttaatcattt
    22441 ctgggcgatc cagttgcaca atttctccct catttaaaat agccacgcgg tctcctaatt
    22501 taatagcctc atcaatatca tgtgtcacaa atacaatcgt catgtcaaat tccttgtgaa
    22561 gactcaacat taactcttgt aattgtttac gtgaaatagg atctaaggct gaaaatggtt
    22621 catccatcaa tagaatttta ggatgagaaa tgatagcacg tacaatacca atacgttgct
    22681 gttcacctcc tgataaatca cttgggtaac gatcaagata atctttagca ggtaggccca
    22741 ccttatccaa taattcttca gttttttgtc taacttcttc aggtgcccac tgcttcatct
    22801 cagggataat cgcaatattt tcagctactg tgagattggg gaaaagagca atttgctgca
    22861 atacgtatcc aatagatagc ctcatctcac gtaaatctaa ctctttctgt ggggcgttat
    22921 ttagtaaaat gtctccagag cttggttcaa taaggcaatt aatcattttt aaaagtgtgg
    22981 tcttgcccga tccgctgggg ccaaccagca caaaaaattc acggtcatta atttgaaagg
    23041 tctgctcttg caaaaccttt gtctgaccaa aagttttaga aacattatta aaacgtatca
    23101 ttatctctcc aattcattgg ttaaagcatc aacataaagg ctgatgagtt cattcaacct
    23161 gtctctctgt cattttttct caaaacgttc tgagtaacta caaggaatca ctgcaggcac
    23221 ttccattttt agacaaacgc atctactaat taaagggact cttagggacc ctcagtaagg
    23281 taaaaactac ccattctcaa aaacattttc tctcattatt ttcttgtcgt ttgttgttga
    23341 ccattataaa aagtttccca ctataagtca aataatgaga ctattacccc cttatagaaa
    23401 aacaggtcac tttgtcagcc tatctctcca aaattacctt atcaaaaatc ctagacttgc
    23461 ctatgatgat agataagcag attaattgtc ttttctagaa aatacagcta tcatacaatt
    23521 atgaaaaatg ccatttccat tttagcgctc ttaacacctg aaaaccaact tgaagagacg
    23581 tttattagag agctttccca ccacttctca cacctttttg aggtgactat tctcactagc
    23641 aaagccaaca ttcagtcaaa tcaattgtcg actttccaag gaattttcag tttccatgag
    23701 catgacattg actttcctac actctatttc aaaacttctc aacatggtca gggcttttta
    23761 gtgacagaaa gtgtctttga tcaagcaaca gcagtcttat cgttaagcca atacctcact
    23821 ggattttacc aaaaatttga tggacatttt ttacagtacc ttcctttgca agcaaggtta
    23881 tctgatgcaa atggaaacat catagtcgat aatcatgctt ttaatggaag ttttttgcca
    23941 actactgata aagaaataga agattggatt cttgctgaat tgcgtttatc agataaccct
    24001 tgtaaaacct tcctacttcc ttcgggttca ttggatcata tttacatgca gcattatcaa
    24061 gctcttaaaa atcctcaagg tcagctggtg ggagttttgg ataccgttca agacattaaa
    24121 ccgttattaa accagtattt agaagaaact ggacaagcca ttgttggctg gtccgatgtc
    24181 acatcaggcc cttccatttt agatcactaa acttgatttt aaatagggag ttcagcctaa
    24241 atcatcaacg cacaagaagc aatcatgaac gcttgaagac ctcaaaacct tcttcttgat
    24301 tgcttttttt gatgactaaa tcggtaagtc tcacttataa agaataatcc ataccagtaa
    24361 accatttttc taaagattat tattcttatg gactcatccc ttgctagtca cgactaactg
    24421 gttgcggtcc ataactttgc gacactggca tcagctcaat acgattaaca ttaacatgag
    24481 ggggctgatg aatcacccat gatacagtcg acgcaatatc ttgaggttga atggcatgtg
    24541 ctccttgata aatggccttc acccgttcct catcaccatt aaagcgaacc tttgaaaatt
    24601 ctgtcccttc acatagaccg ggttcaatat tggtcacacg tatatggcta cctgctaaat
    24661 cagctcgtaa attgagtgag aactgcttaa caaaagcctt gctagcccca taaatattag
    24721 ctcccggata gggaattgtc cctgcggttg accctaaatt aataataata ccatcatctt
    24781 tggagaccat atgtggtaat aattgacgtg tgagataaat caacccaaca atattggtat
    24841 taatcatcgt catccaattc tcaaaatcgg cttcgtaagc cttatcaaat cccaatgcta
    24901 agcctgcatt attcactaaa atagtaatat cttgccactg atttggtaga cttgtcaaag
    24961 catcatcaat cgctttggta tctgtgacat ccatctgcaa agggtaaaag ttgtccgacc
    25021 caaagctcgt ttctaagacc ttgagtttct caagacgacg agaagcacca ataaccttta
    25081 tcccatctgc aattaagtgt tcgacaatgg ctctgccaaa accggctgaa gcccctgtta
    25141 ctaaggcaat atttcccata catttcctca ccttcaaaaa aaacgagatg gtctggccaa
    25201 ctcgttttta ataatgtcaa ttagtcttag gacaacttct aacacatcac ttagcggtta
    25261 gaatcctata tcaaggggtc ttaagttacc cttggctttc gatagcttgt gctgctgtaa
    25321 taatagctaa tttgtaaata tcttcagcgc ttgagccacg agataaatca ttaacaggtt
    25381 tgttcaatcc ttgtaagatt ggtccaatag catcaaacat tcctaaacgt tgtgcaattt
    25441 tgtaaccaat atttccagat tgtaagtcag ggaaaataaa agtattggct tggccagcaa
    25501 ccgctgaatc tggtgctttg atagctgccg tttcaggaac aaaggctgca tcaaactgca
    25561 attcaccatc aagagctaag tcaggattca accctttagc aatttctgtt gcttctctta
    25621 ccttatcaac ttgaggcgct ttaccagaac cttttgttga gaagcttaac atagcaattt
    25681 taggatcaat atcaaagatt ttagctgttt cagctgtgtt aacagcgatt tctgccaatt
    25741 cttgtgctgt tgggtcaata ttgatagcac aatcagcaaa cacgtaacgc tcactcgtat
    25801 tttcacgatt catcaagaaa acacctgaag ttcttgaaat acctggtttg gttttaatga
    25861 tttgaagggc tggacgaaca gtatcagctg ttgaatgaat agcaccagat accattccat
    25921 ctgctaatcc cattttaacc aacataacac caaagtaatt cacatcacga agcattttat
    25981 cagcatcttc aagcgttgct tttcctttac ggatgtcaac aaaagtttct ttcattttgt
    26041 caaaatccgc atattcattt ggattaatga tagtatagtc ttgatcagcg aagcctaatt
    26101 ttgtcaaaag gttgcgtacc tcttctgact gtccaaggat aatcggttct aagagtcctt
    26161 caaatttcaa acgagcagct gcacggacga cgcgctcatc attaccttct gggaaaacaa
    26221 tcttcatgtt tttaccaaga attttttctc ttaaaccacc gaataaacta cgaatactca
    26281 ttctttaata acctctatct tttttctgta aacctatgat aacgctatca aagtcatctg
    26341 tcaagtgtat tgcatagcta caatcacttt ctgtaaatgg atctttgaaa ttcaaataat
    26401 gacaatgtaa ggcttgcctt gttattccta agtccaaacg acctccgtaa aggtcatccc
    26461 ctaaaagtgg aaaaccaata tgggcaaaat gaaccctgat ttgatgggta cgtcctgtat
    26521 gcaatttgat atccactaaa tgcacatttt ctgaatagcg cgccacaacc ttatagctag
    26581 tcttggcata tttgcccata gggtcaactg ccctggtaat aatactatct tttgaacgcc
    26641 caattggagc aataatatct ccttcatctg gcaacgtccc atttccagaa accagagcaa
    26701 aataacgttt ctctattgag cgagtttgga gttgcttgtc caaccttgca tgagcataac
    26761 catgcttagc aaataacatc aagccactcg tatctctgtc taaacgggtc acgatatgta
    26821 cttgttggtc taggtaatga ttgtccacat aatatgcctt gataaagtta gcaatggtat
    26881 tggaatggat ggcacttgga atactggcaa agccaatggg tttattaatc accaaaaaat
    26941 gatcatcctc gtgaacaata tccaaatcaa aagggatggc ttcaagcttt tcaaagggct
    27001 cttcgtttgg aatgtcaatg gtgacaacat ctcccacttg caataaataa atggcatttt
    27061 gttcaatacc attcactaat atattaccac ccttatattt gattttagcc aatagtcctt
    27121 tagagacatc atgacttttt aaaagggttt ttacttttat tcgtttgtct gctacaaatt
    27181 caaatctcat gagtcaattt ctccaataaa ggcatccttg accctttccc aaaagctcgt
    27241 atgactcgga gaggaaacaa aatgaatttt ctcatcgtcg ataaaatact ccaccttcgt
    27301 cacatttttt aactgatagg ttttattatc aatggaaatg gtataaattc ctaatcgttt
    27361 tggcactaac tcaatcttat cttttttggg aataatgatt gatgagccca aggttctaaa
    27421 gacacggtta ttaagactgg aaatttccgt caattgcagc gcttcaatcg tcggatgcaa
    27481 gacagcacca cctaaagatt tattgtaggc tgtgctccct gtcggggttg atactgaaat
    27541 cccatcacct cggaagcttt caaatttgac atggttaata ataacatctg ctaccatcgt
    27601 tttttcaata cgcttaaccg tcgcttcatt caaagcacgc gctttaacca cacgaccatc
    27661 atctaaagta atagcaactt ttaaaatagg ataagagatt tgttctccct tgtcttttct
    27721 taaattatca atcaatttat caacttcaaa atccctataa tcggtataaa agccaagatg
    27781 accggtgtgg attcctacaa aacgtacctt atctaattct ttttcataca tgtgaaaggc
    27841 agacaagagc atcccatctc cgccaataga aatcacaata tccggatttt tctttgaaag
    27901 atagaaatca ggatcatctt taaatacgga gaaaagtttg gaggcgacgc gtttactttg
    27961 gtacttacca tttgcaataa tagcaactcg ctttacctta cctgtataat tcatctgtgt
    28021 catcactatt tcctacacca tcacttaaat ttcgtgtcac ggggtcaaac aataattgag
    28081 cctcccgaat atcttctcga atcttacgca tttcttcgtc taattctaaa gcaattttag
    28141 cagtcacttc aagacgcttt ttaatttctt ctgggaagtc tccaccatat ttataattta
    28201 aagaatgctc aatggtcgcc caaaagttca ttgccaaagt acgaatctga atctccgcta
    28261 agaccttttt ttgaccttca atagtatcaa cagggtactc aaccaccaca tgataagacc
    28321 tgtagccact ggacttcata ttgcgaatat aatctcgttc ataaacaatt gtcatgtctt
    28381 gtcgttgacg caatagagct aagacttctt ccacatcatc aacaaactga accataatac
    28441 gtagtccagc aatatcttga atatcttgtg ctatattttc ttcaataaca ccacgtaaaa
    28501 tcattttttc tttaatactt tcaattgact taacacgtcc cgtaacaaat tcaatcggag
    28561 aataacgatt ttgcttacga tattgcttgc gaataccccg tagttttatt tttaattcac
    28621 caactgtttg gatataagga tctaaaaatt cttcccaatc taaggtcatc tattgtctcc
    28681 tttcttgtca aagccaggat aataatataa aattgaaata acaatttatt atagcacaaa
    28741 ttaaaaggaa tttcccatga ctaacctaga aatcgaatat aaaactctac ttacaaaaaa
    28801 tgagtataac cgtcttttat ctcaaatgaa acatgtgacg cctgttacac aaactaatta
    28861 ctatattgat acaaaagcat ttgatttaaa agctaacaaa atgtcattac gtattcgaac
    28921 atttgtaaat agcgccgaat taaccctaaa agtgccagaa aaagtgggga atcgtgagta
    28981 caatgtccct ctttttcttg aacaagcaaa agatatgatt aaacatggga atcttcctga
    29041 atcaactgct cttgatatta ttatttcaaa aggtatcaaa ccatctgcac tggtaacttt
    29101 tggcaattta actaccgttc gtcgcgagac agtgataccg ataggtaaat tggccttaga
    29161 ctataaccta tatgccaata ccaaagacta tgaattagaa ttagaagtaa gtgacgctct
    29221 tcaaggaaag attgattttg attctttttt atctgaacat cacattactt tcaaatatgc
    29281 taaaagtaaa gtagctcggt gtattaatac cttaaaaaag ttcaacgata aataatggct
    29341 aaaagcgaca gaaaaactga aatttttctg tttttctgtt aaaataggga tgtcaattaa
    29401 acacttttgg gtaatgtgac tcattaataa aacagtttac ccaattttat tgaagttaag
    29461 gagctaaatc tatcatgacc gaacgatatg ctgacaagca aatcaaactg ttctcactca
    29521 catcaaatct tccaattgct gaaaaaattg ctaaagctgc tggaatccct cttggaaaaa
    29581 tgtcttcacg tcaattttcc gatggagaaa ttatgatcaa tattgaagaa acagtgcgtg
    29641 gagatgatat ctatattatt caatccacta gttttcctgt caatgataat ctttgggaat
    29701 tactcatcat gattgatgct tgtaaacgtg caagtgctaa taccgtcaat attgtattgc
    29761 catactttgg ttactctcgt caagaccgtg tcgctaagcc ccgcgaaccg attactgcta
    29821 aactagtagc taatatgttg actaaagctg gtattgatcg tgtagtgacg cttgacttac
    29881 atgctgttca ggtacaaggt ttttttgata ttccagtgga taacctcttt acagtccctc
    29941 tttttgcaga acgttacagt aaattaggct tatcaggttc tgatgttgtt gtcgttagcc
    30001 ctaaaaattc tggaattaaa cgtgctagaa gcttggctga atatcttgat tccccgattg
    30061 ctattatcga ctatgcacaa gatgattctg aacgtgaaca aggttatatt attggtgatg
    30121 tttctggtaa aaaagccatt ttaattgatg atattttaaa tactgggaaa acctttgcag
    30181 aagctgctaa aattcttgaa cgctcaggcg ctactgatac ttacgcggtc gctagccatg
    30241 gcttatttgc tggtggagct gctgaggtat tagaaacagc tccaatcaaa gaaattatcg
    30301 ttacagattc tgttaaaact aaaaatcgtg tgccagaaaa cgttacttat cttagcgcca
    30361 gtgatttaat cgcagaagct attattcgta tccatgaaag aagaccctta agtccacttt
    30421 tttcttatca acctaaaggc aaaaataacg catgacttac tttgataacg ccgccaccac
    30481 accacttagt cctaatgtga ttagggcgat gacagcagct atgcaagata actttggtaa
    30541 cccctctagt attcatttct acgggcgccg agccaataaa atccttcgtg aatgccgaca
    30601 agctatcgct agaaacttgg gggcaagtga acagcaaatt atagtaacgt ccggtggaac
    30661 tgaaagcaat aatatggcca taaaaggcta tgctttagct catcaagcaa agggcaaaca
    30721 tctcatcaca actactattg agcaccactc tgtgctcaat accatggcct atctcgaaga
    30781 gcgatttggt tttgaggtca cttatttgcc ttgtcaaaat ggacaaataa atttatctga
    30841 cttaaaacaa gccttgagag atgacaccat tttggtaagc ataatgtatg ccaataacga
    30901 aacaggtgac ctgttaccta ttaaggacat tgggaatctg ctcaaagacc atcaagctgc
    30961 ttttcatgtt gatgccgttc aagctgttgg caaactcaag attattccta gcgaacttgg
    31021 gattgacttt ttatctgctt ctgctcacaa atttcatggt cctaaaggat gcggcttttt
    31081 atacagtaac ggacaaccaa tcgatcctct tcttcacggg ggtgatcaag aaggaaagcg
    31141 acgcgcaagt actgaaaata tgttaggtat tattggtatg gcacaagctt tgactgatgc
    31201 tatgacttgt cttgaccaat caactgatca cattattagt ttacgtcatc acttgattag
    31261 cctcctagaa ggactaccct actatattaa tcaaggtact cattatcttc ctcatgttct
    31321 caatattgga tttttaggct accaaaatac tattttacta acacaacttg acttagcagg
    31381 cattgcagta tcaactggtt ctgcttgtac tgctggagct gttaatccaa gtcatgtttt
    31441 agctgcgtac tatggggatg actcttctcg tttaaaagaa tcaattcgta tcagtttttc
    31501 agatcaaaat agcattgaag atgttaatca attagctcaa acattaaaaa atattttagg
    31561 aggaacagat ggcttttgaa aaagaaatta agcttaaaga ctgtaaatac ctttatacaa
    31621 tcagtccaaa cattaaaaaa tataccctaa gagatactac atttagtcaa actaaagtag
    31681 ggcactacca attaatacgt ttactcgaaa caatacctaa ttcaggggat ggtttccctt
    31741 taaaaatcac tattaataaa gaactcacag gttttaaatt atctatc
//
LOCUS       DATURV010000014        30445 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DATURV010000014 DATURV010000000
VERSION     DATURV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30445
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.014"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..1110)
                     /locus_tag="VCF75_000635"
     CDS             complement(<1..1110)
                     /locus_tag="VCF75_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEP2546219.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVE"
     gene            complement(1486..3291)
                     /gene="pepF"
                     /locus_tag="VCF75_000636"
     CDS             complement(1486..3291)
                     /gene="pepF"
                     /locus_tag="VCF75_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP2546220.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFS
                     GHLLDSSANLLTITDTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            complement(3304..4266)
                     /locus_tag="VCF75_000637"
     CDS             complement(3304..4266)
                     /locus_tag="VCF75_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEP2546221.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFRCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     CLSFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            complement(4562..5278)
                     /locus_tag="VCF75_000638"
     CDS             complement(4562..5278)
                     /locus_tag="VCF75_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP2546222.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            complement(5397..6101)
                     /gene="nagB"
                     /locus_tag="VCF75_000639"
     CDS             complement(5397..6101)
                     /gene="nagB"
                     /locus_tag="VCF75_000639"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEP2546223.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIEANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            6303..7331
                     /gene="queA"
                     /locus_tag="VCF75_000640"
     CDS             6303..7331
                     /gene="queA"
                     /locus_tag="VCF75_000640"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEP2546224.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSG
                     WTNIFIKPGYQFNVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            7338..8558
                     /locus_tag="VCF75_000641"
     CDS             7338..8558
                     /locus_tag="VCF75_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEP2546225.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQDLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSALIQACYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLIHHY
                     QETRGDNKTSKTQH"
     gene            complement(8712..9302)
                     /locus_tag="VCF75_000642"
     CDS             complement(8712..9302)
                     /locus_tag="VCF75_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEP2546226.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVCSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQVLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLIALYSSGL
                     GGATQASEEVNGLGYVQLVQLTYVINEWHRVNHNMI"
     gene            complement(9527..9760)
                     /locus_tag="VCF75_000643"
     CDS             complement(9527..9760)
                     /locus_tag="VCF75_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546227.1"
                     /translation="MLREKKTTIVGIILGMMIVTLLVGCLTGKKKGFPPKAGERQQTA
                     DLKMKSKIRKTVQKPLPEKSKEAKQAVMDADLA"
     gene            complement(9927..10532)
                     /locus_tag="VCF75_000644"
     CDS             complement(9927..10532)
                     /locus_tag="VCF75_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEP2546228.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            complement(10629..11669)
                     /locus_tag="VCF75_000645"
     CDS             complement(10629..11669)
                     /locus_tag="VCF75_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEP2546229.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSYQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILGRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            complement(11740..13983)
                     /locus_tag="VCF75_000646"
     CDS             complement(11740..13983)
                     /locus_tag="VCF75_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEP2546230.1"
                     /translation="MTFSWTKLVPLSKIQFAFLILVFFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDKSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FASFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRREYHVANAQKTLIPYLKSRGIHTIDQLLVTHADTDHMGD
                     IEVVAKAIRIKEILTSQGSLSHPIFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPW
                     QLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTS
                     SSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDRQITYYRTDTQGAIRLTGWTS
                     WHLETVR"
     gene            complement(13964..14626)
                     /locus_tag="VCF75_000647"
     CDS             complement(13964..14626)
                     /locus_tag="VCF75_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEP2546231.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            complement(14826..15566)
                     /locus_tag="VCF75_000648"
     CDS             complement(14826..15566)
                     /locus_tag="VCF75_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEP2546232.1"
                     /translation="MFYAYLRGLVVFLLWIVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKMAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            15606..16460
                     /locus_tag="VCF75_000649"
     CDS             15606..16460
                     /locus_tag="VCF75_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEP2546233.1"
                     /translation="MKVDFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            16450..16728
                     /locus_tag="VCF75_000650"
     CDS             16450..16728
                     /locus_tag="VCF75_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEP2546234.1"
                     /translation="MTTKKAYMYVLECADKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(16752..18752)
                     /locus_tag="VCF75_000651"
     CDS             complement(16752..18752)
                     /locus_tag="VCF75_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEP2546235.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNMLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLTVYVVILATLAAIIASQALISGSFTLVSEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLYFRTSAHMEAAYGLAITITMLMTTILLNYYL
                     IKEGVKPFLAHLVMTFFALVEFIFFWASAVKFMHGGYVVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVRFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDKPYTAKYKVDMMGTDYMVRVELYLGFRMPQTVPRYLRTIVHD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNVRQLTNVERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(18879..20498)
                     /locus_tag="VCF75_000652"
     CDS             complement(18879..20498)
                     /locus_tag="VCF75_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2546236.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            complement(20805..22349)
                     /locus_tag="VCF75_000653"
     CDS             complement(20805..22349)
                     /locus_tag="VCF75_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEP2546237.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLTKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTSKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            complement(22597..23292)
                     /locus_tag="VCF75_000654"
     CDS             complement(22597..23292)
                     /locus_tag="VCF75_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEP2546238.1"
                     /translation="MDFFAIDPIGLPHISLIFYLLSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            complement(23372..24763)
                     /locus_tag="VCF75_000655"
     CDS             complement(23372..24763)
                     /locus_tag="VCF75_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEP2546239.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDICLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVD
                     RLVTDKNCDSYQP"
     gene            complement(24954..26000)
                     /locus_tag="VCF75_000656"
     CDS             complement(24954..26000)
                     /locus_tag="VCF75_000656"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEP2546240.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            complement(26101..26697)
                     /gene="recR"
                     /locus_tag="VCF75_000657"
     CDS             complement(26101..26697)
                     /gene="recR"
                     /locus_tag="VCF75_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEP2546241.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(27494..28273)
                     /locus_tag="VCF75_000658"
     CDS             complement(27494..28273)
                     /locus_tag="VCF75_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEP2546242.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFATNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            complement(28397..28939)
                     /locus_tag="VCF75_000659"
     CDS             complement(28397..28939)
                     /locus_tag="VCF75_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546243.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            complement(29100..29621)
                     /locus_tag="VCF75_000660"
     CDS             complement(29100..29621)
                     /locus_tag="VCF75_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEP2546244.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            complement(29728..30423)
                     /locus_tag="VCF75_000661"
     CDS             complement(29728..30423)
                     /locus_tag="VCF75_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEP2546245.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
BASE COUNT         9601 a         6306 c         5334 g         9204 t
ORIGIN      
        1 ttcaacagtg atagataata acataggtcc agttaatgca gctattgccc aagctgtcaa
       61 tatataccca tgtaatgtag ccagttcctt tgccccaaaa agatcactta aataaggcgg
      121 aatgagagaa aaacccgcac cataacaagt cataagggtg gcaatgctta tcatgaaaat
      181 tagggaacta tgtgcaaata ttaaactaat agtcataatt atgcttacta aaaataaaag
      241 tattactgtc acacggcgtc caatatagtc tgacaaacta gcccacacta aacgaccgaa
      301 accgttaaaa atccccatag caccaaccac aattgctgac atttctggac tcataccagt
      361 tagatcttgt gccattggcg caacaactga gataagtcct aagccacatg tgatattgat
      421 aaataaaatt acccaaagac aataaaatga ttttgtttta agtgcttctt tagcggtcat
      481 tccttcgatt aaataactgt tattttgtaa tcttttttta tctagtatag ctatctcagc
      541 agcagttggt tttattatca attgagaagc aaaaagcata actattagat aaatcagccc
      601 taaaaggtaa aatgttgcta ccaaaccttc tgtttcaatt agccactggg caatcggact
      661 tgttaataaa gacgcaaaac caaaccccat aatagcaaac cctgttgcca tgcctctttt
      721 atctggaaac cattttataa tagttgatat cggtgtgata tatcccgctc caagtcctaa
      781 gccaccaata acaccataac caatatataa aagccaaatt tctttacgat caatcgctaa
      841 accagtcagc atatttccag aagcataaag aatagccgac accgttccgg ttagacgagg
      901 accatattgt tcaactaaat ttcccataaa agcagcagat aaacctaaac aaaaaatagc
      961 taaagaaaag gcaaaagcga caggagcttg atcccaaccg gtctcttgta aaattggatt
     1021 acgataaaca ctccaagcat aggtcgatcc caacattaag tggagaagta tccctgctgt
     1081 tgcaatgata taacgttttg ttttttccat aaaaaactcc ttttttatta tacgaacgtt
     1141 tattttttaa aaaacataaa ggttagtatt ttaattctat tatctctttt ataaaatgtc
     1201 aaatataata aaaacatgtt ttatacgaac gttatctcta aaacaatgga atggcgttaa
     1261 tctgaagcgc cttactcaac tattggtttt atttaagtga gtgaccacac aataaaaaag
     1321 acaaaaaaga aaccgtcaaa ctaaggtcaa tgaagcctgc ttttcaggac tgataatcca
     1381 ctaaaatata aaaagaaaat gaccgataga aatggagatt cacaccaact gtgaatctcc
     1441 atttctatca atcctaaact ctttttgtcc ttgacttttg tgattttagc ttttgtgtat
     1501 gcctttggac acgagtactt caagttctgt tagacgttcg tcaaagactt taaaggcagc
     1561 ttccaaataa tcaccttttg ccatatcaac gcctgcttta gcaatcactt caagtgggta
     1621 atcagaattt cctgacttga ggtaggccaa gtaatgatcg atatcatctt gtgtgccatg
     1681 cacaatctta tccgctaaat agcttgcagc tgcaaaccct gttgcatatt ggtaaacata
     1741 ataattgtaa tagaaatgag gaatacgtgc ccattcatac tgaataaaat gattatcttt
     1801 tttacttaaa ccataatatt tttcatttaa atcagcgtat agctggttaa ggtattcact
     1861 tgttaacact tctccttttt gatcagcttg atgaatggca tgttcaaact cagcaaattg
     1921 ggtttgacgg aaaactgttc cacggaaacc atcgaggtaa tggtttaaaa tggcaaagcg
     1981 ttctttctca tcttgcactt catttaagag agcttctgtc atgatgtttt cattcgttgt
     2041 ggatgcaatt tctgccaaga agatactata atccccatac acataaggct gtgtttcacg
     2101 agtgaaagta gaatgcaaac tgtggcctgt ttcgtggaca agtgtgtaaa gattatcaag
     2161 tgtatcttgc cagttcaaca gcatgaaggc attggtatca taggaaccac ctgaataagc
     2221 cccagagcgc ttccctttat tgacgtgaac atcaatccaa cgttcggtaa aagcacgatg
     2281 tacacgatcc gcgtaatcct taccaaaaac agccaaaacc ttctcagcct tttccaaagc
     2341 ctcatcataa ccaatggcta aatctgtttc agataatggt gtgtaaacat catacatctt
     2401 caaatcgtct aaaccaagaa cctcttgacg caatttcaaa taacggtgta agagtggcaa
     2461 atgtttatta accgtttcta acaaggtatc gtaaacagct tctggaataa agttagcagc
     2521 catggcagct tgacgtgctg aatcgtattt atgaacacgc gccttatagt tttgcacttt
     2581 cacattggtt tgcaaggttt tagcgtaagt atgttggaac tgttcataag tactatacat
     2641 ggcttcataa gcagcttggc gaatgctacg gtccttagat tccattaggc taatgaagtt
     2701 accatgggtt aattcaacat cttccccctt atcattttta actactggaa agacaatatc
     2761 agcgttatca agaatactaa aggtttcttc agcgccgttg aaaatttctt gggctcctgc
     2821 taataattcc tcttcagcct ggctgagcac atggtcacgt gtctggaaca atttgtcaaa
     2881 gaaatgatga taccttttta aatcaggtgt ttccgcaaga aaagcttggt aatcttcctg
     2941 actcaatgcc ataacttctg ggtcatagaa tgaaaacact tcgctaaact ttgcatacaa
     3001 gcctgaagcc ttagcttggt attcttgata cttggcaaca gtagtgtctt ggtcattttt
     3061 catgtgagca taaacataga ctttctcaac acgacgagct aactctaaat aagtatctgt
     3121 aattgtcaac agattagcgc tagaatctag taaatgacct gaaaaatcct tagccgcgtc
     3181 aatttcagta gctaagtcac tgacttcagc ttcccaatcc ttatctgttg caaaaatagt
     3241 gctcaaatcc caagtgtatt tttcttctaa atggctgcga ttatcagtca taacatctcc
     3301 ttctcatttt ctattactat ccattttaac ataatatttt ggtgaaaaaa gcgtttgcac
     3361 cttcttatcc ttttcttttt tataatagac tttaaaactt tcataataag ggcgaagatt
     3421 tcctttaatt tgacaaaaac cgctggaaga ttgaactggt ctccattgag gataaaaagc
     3481 atcagttgtt aacataagta aattatagcc tgataagtaa gccttttctt gtcgtcgtag
     3541 ccatttcggg tgtctagcta aaagagctct ttgtattctt ttagataggt tcacagtcat
     3601 ttttttctga taagaataaa ggatttcttg ctgatagggt aatcgaaaca tttccataat
     3661 attgtggtct aaagaaatgg ttttggttag gtaacttacc ttaccaaata agtcttcatg
     3721 gattaaatat ttgagacgta gcagatttgc agctgcatcc agctcccaca aatgaaagct
     3781 caaacaagat gaaaagtaaa gaaactgttt ttgcaaagca gttaagtgag tgttaagcca
     3841 caattttctt cccagtaacc atcgtaccgg ataccccttt tcttgatagg ctttggtgcg
     3901 tttctttaga cgctcaactg gcaagggact gcactgaatt tctaaggcta acttatcatt
     3961 cacccaaagg tctgctatct gttgcagctc aggaagatat ttttcaatac agacagcttc
     4021 tgttcggact aaactggtgt aaagttttgc cttcaacgtc aaatgttcct cagattcatt
     4081 ttctgcttgg aattgacaat gagctagttg tacatgggca aaatgaggtc gtctaattgt
     4141 cccctgacgc aagcgaacag gagatttgca agctgggcaa cgaaaaggcg gtttcgttga
     4201 aataggctga gttactagcg aaatgagctg attcttacca tctaaagcgg ttaatatttt
     4261 tgtcacctcc tattagatct tccaccttat tattcgaaaa ctaagtacca aactttaagt
     4321 agctttagtt tttttagcca tcaaaaaaca ctcccatttc caattttatg agagtttaac
     4381 acaagggttt ttataacgct tcaggttaag gagatagtgg gaattgaatc tttttgctta
     4441 tctgattttg taacaacaag atgactaaac atagcaaaag tcggactcat catgactata
     4501 atagtcaatg gacactttat tgattaagca agaatagctt ctttttgaaa gcatgttttg
     4561 ctcagtccag atacgtttta atgagttgcc tttctgacgc tgttagataa cgatattctc
     4621 cgctggctaa atgaccgagt tccagccctg caaagtgcgt ccgtttcaga gaggtcactt
     4681 tcaaaccata ggtcaaaaac atttttttca cttggtgaaa ttttccttct gaaatggtca
     4741 aactagcctg tgactggtct gtatcggctt taagaatagt caattgagct ggttggcatt
     4801 gttcacctgt tgggaaacaa ataccagctg caaaaaaatc tgaggcatct tctgctaaca
     4861 agccattaac ggttacaaga tacgttttag aaacatgatg actagggtgc aacatccgaa
     4921 accctagggg accattatca gtgagtaaaa ccaacccttc ggtatctcta tccaatctgc
     4981 caacaggata caagtctgga cttttatctt cttcagcaag ctgatcaata acggttaaat
     5041 agttagtatc tttcttagct gagaccaccc ctgatggctt gttgaggata atgtaagaat
     5101 gtttaggatg cgtcactcgc tgtccagtca cctcaatcag ttgtaagcca ggatcaacat
     5161 tttgtcgacc atttctggca ggcatgtgat caacccaaac cccttgagcc ttaatcagtt
     5221 tcttcacttg acttctagag ccaacttttg taccttctaa taatttatct aaacgcatat
     5281 tagtagtgta acataaattt acccttgcaa aagaaataga agttacaaga cttcttctta
     5341 aatgaggttg actatctcct catttttgtt cttacaaggt ttaacgactg gttaggttag
     5401 tctaattgtg acgcagcagc ttcatccaca atgacgataa catgatcatg tttttggaga
     5461 atacttgctg ggaggtcttc tgtgattggt ccaaaaacca ttcccttgat cgcatcagct
     5521 ttttcttgtc caaaggcaag aagaacaatc attttggact tcatgatgga ggcaattccc
     5581 atagagatag cttgttttgg cacatcctca atacttgtaa agaaacgact attggcttca
     5641 atggttgatt cttgaagatc caccacatgc gtttcttctt caaaagaggt cccaggttcg
     5701 ttaaaaccaa tgtgcccatt acgcccaatc ccaagcactt gaaaatcaat cggatgctct
     5761 gcaatgattt ggtcatatcg tttagcttca gcttcgatat cagttgctaa accattgggc
     5821 aagtaattct ttttaaaagg cttcgcatta aaaaggtttt gacgcataaa ataatcataa
     5881 ctctgatcgc tctctactga aagaccgaca tattcatcca agttaatact tgttaagtcc
     5941 gaaaaatcca aaggactttt gaccatttct tggtaaaaac taattggtga acttcctgtt
     6001 gctaatccta acgtcttagc tcccttagct aagctatctt tcaataaggt aaaagcgatt
     6061 ttcccacctt caatctgatc ttggacccga ataattttca tgtcattctc cttttctaat
     6121 tggtatagac ctttcgtgtt tttattatat ggtatatacc aatttttgtc aagcctatcc
     6181 tttactttct gatgaaagat taaagatgac gggttagacg acaagtgata taatagaaaa
     6241 gatttatctt acatgaaaga gttactagga agagatttac tagtggcttt aaaggagccc
     6301 caatgaatac taacgatttt gattttgagt taccagaaga attgattgcc caaaccccac
     6361 tcgaaaaaag agacagttca aaactattga ttattgatca ccgtcaaaaa actatggttg
     6421 atagccattt tgaccatatc attgatcaat taaatccagg ggatgccctc gtcatgaata
     6481 acacccgtgt cttgcctgcc agactttatg gagaaaaacc cgatactcat ggtcatgtgg
     6541 aattgctttt gcttaaaaat acacaaggag atcaatggga agttttagcc aagccagcca
     6601 aacggctcaa ggttggtagc caagtcaatt ttggagatgg gcgtctcaaa gccaccatta
     6661 tcgacgaatt agaacatggt ggacgaattg ttgaattcag ctatgatggg attttcttag
     6721 aagttttaga gagcctcggc gaaatgccat tgcctcctta tattcacgaa aaattagagg
     6781 atgctgagcg ataccaaacc gtttatgcta aagaaaatgg ctctgcggcc gccccaactg
     6841 ctggtctcca cttcacaaca gacttgctca aaaaaatcga agccaaaggg gtacatttgg
     6901 tttatttgac cctacatgta gggcttggga cttttagacc tgtttccgtg gataacctcg
     6961 atgagcatga tatgcattcg gaattttatt ccctctcaga agaagcagcg caaaccctaa
     7021 gagacgtcaa acaagctggc ggtcgtgttg tcgctgtagg aaccacctct attcgtacgt
     7081 tggaaaccat cggaagtaag ttccaagggg acattcaagc tgattcaggt tggaccaata
     7141 tttttattaa accaggctac caattcaacg tagttgatgc tttttcaact aatttccatc
     7201 ttccaaaatc aacccttgtg atgctagtat cagcctttgc agggcgtgac tttgtccttg
     7261 aagcttacag acacgcggtt gacgaaaagt atcgtttctt tagctttggc gatgccatgt
     7321 ttgtcaacta aaaacctatg aaattacttg gaaaatggct attcttcttg atgtttctga
     7381 ctttttgcgt cagtttttgg cagaaagact atcagcggat tgcctttgaa aaagaacaca
     7441 aactttatca agatttagtg gcaccttttt accaaaacta tcctaaatta cgagagctcc
     7501 agctttcaga agctattaaa ttacgccacg acttgcttga ttatgttcga aaactcgata
     7561 aggacggatg gtcctataaa gccatccaaa aaggatacct tgaacaactc gctgttagag
     7621 gaaactctta tcatttcgaa caactttata gtcgcattcg cctgatagga tcacctgact
     7681 ttcaaaagct ctggcaacaa gaagagatgg cacagacgcc tcaagaagct caaaaacggc
     7741 tacagctgct cttgacgtat ttaaagatgc cagacgagtt gacaggacaa tcaaaacaaa
     7801 cacagcaagt attggcacaa ttaagtccaa agctatcgcc aacagatcct ttttgggacc
     7861 aattatctgc cctgattcag gcttgttatg ttaatctcga acacattcct tatagccgtt
     7921 ttaatcggaa aattcatcaa ctccgttacc tcatatcaac ccagcaaacg gagtgggttc
     7981 gctcacatta tgggaataaa gggaaaaccg atgcggatgc cttagccaaa tatttggcta
     8041 ctctagaaga tgacgattat gctctttatg aatcttctag ataccataat aaggttgcta
     8101 gtattttaga tgctaatggc aatcatcagg cagtctatac agacaatatt cctcaagcca
     8161 actataagat tttgattcat tttcactcag agtttatttt gtcagaatct ggacaatttt
     8221 tagtagcact agatcctgat aacctaacgc gaaatagtat tgtcaatggt tcttctttca
     8281 attatggcaa ccaaaacgat gacttgcacc gtctgcttga tattgaccct attctgcttt
     8341 ttgatccggc ttttattgaa aaagcaatta atagtcctga tgcaactttt ctagttcccg
     8401 atttagaaca gcaaagggat aagcacaatc ccatttacag cagaaatggc aaatcatcta
     8461 agcagctaac acgcgctgcc gttaaaaaat tcaaaaaatt gattcaccat tatcaagaaa
     8521 caagaggtga caataagacc tcaaaaacac agcattaatc tcaaaaacac agcattaatc
     8581 tcaaaaacac agcattaatc tcaaaaagct tgacctgttt cttcaggtca agctttttta
     8641 atgagcaaaa acttatttta gtgactgtca tcagtaagta tgtctactat cagactaatg
     8701 tcaactgagt attagatcat gttgtgattg actctatgcc actcattaat tacatatgtt
     8761 agctgaacaa gctgcacata accaaggcca ttgacttcct ctgatgcttg tgttgctcca
     8821 cccaaaccag atgaataaag agcaatcaaa taaggagact cttcgcaaac aatggcacct
     8881 acatttaagg cttctctgac ataacccggt ttttgataaa cctttacatg aggcaaataa
     8941 gtcttgtaat acaaatcagg gaaagattca ccaatgtagt acaagatatc cttatattta
     9001 tcctgatgct gccacaagta atccaaaact tgcaaatagt aagcggtggt gattttattg
     9061 ccattacgat caatggtcgt aatagccccg gataccttcc cgtagcggtc aaataattgg
     9121 taagccttat tcatgccacc caatcgttct gctagagcat aagctggcgt gttttctgag
     9181 cacaccaagg aatattcttg catatccgga atagacatgg caccattgaa ttgagcaaca
     9241 taggcatgat gttcctgatc atattcataa tcagtttctg taatgtcaaa acgctctgtc
     9301 atggataatt tgccttatct accaccggca tgtttaatgg cagcttatag gtgcttcctg
     9361 caatcattgg ctgatggtca ttcatggcag cagtcctacc tgaattgagg tctttgtagg
     9421 aaaaggcaat ttgggattca ccaatgccaa attcctttaa atacgccttg accgtattga
     9481 ccaaactcaa atggttgtca tcgtaataga gccctaaggc atcgatttac gctaaatctg
     9541 catccataac agcttgctta gcctctttag atttttcagg taacggtttt tgaacagttt
     9601 ttctaatctt gcttttcatt tttaagtcag ccgtttgctg ccgctcacct gctttaggag
     9661 gaaagccttt ctttttacca gtcaaacagc cgacaagtaa ggtcactatc atcattccta
     9721 aaataatccc tacaatggta gttttcttct ctcttaacac ctaaaacctc ctatccattc
     9781 tattctagct aaatttagct aaaaatcaag gaattacatt aaaaagacaa actattttta
     9841 tcttttctaa aaaatgatgt ccccacactg ttattcacaa aagcacttaa aaaaccaatc
     9901 tctttttgag attggaaacc cttttcttat ttagcggctt ggtaaagttc tgatactttt
     9961 ttccaattaa tgatttcaaa gaaagctttg atataatttg gacgtacatt acgataattc
    10021 aagtagtaag catgctccca cacatcaagc gctagaattg gttttttgcc ttctgaaatt
    10081 ggagtatctt ggttagcggt tgatgtgatt tcgagttgtc cttctttatt aacaactaac
    10141 catgcccaac ctgaaccaaa acgtccagta gcagctgctg taaattgttc tttaaaggcg
    10201 tcaaatgacc caaaagcatc atcaatggct tgtgcaacat caggagtgac gtcttgtttt
    10261 tcaggtgata atagttccca gaaaagcgca tggtttaggt gtccgccacc attattaatt
    10321 aaggcttgac gaatatcttc tggaatctta gtgacatctg ccaatagttc ctccagattt
    10381 tctccgatct ctgggtgttt ttctaaagcc gcattggtat tagcgacata agtggcatgg
    10441 tgtttatcat gatgaagagt cattgtctcc gcatcaaact gtggttcaag agcatcatac
    10501 gcgtatggaa gttctggtaa aataatagcc attttcctgt cctcttttcc tttatgatac
    10561 gtctatctta tcgcaaaaaa taagagcttt caactaattt gattatctct tggaaagcgg
    10621 tccaacgcct atttttgaga gtgagtcatg atttttaaga gagcaatatc aactagataa
    10681 ctcttctcat aaagtcctgt ttttatctgg taatctgtct caatcaaggt tttcaccgct
    10741 cctgttagaa aggcaagaga taaggtccta gaatccttta acgcatactt gacctggtaa
    10801 ggattaaccc gccgaccaag tatctctgac aaactggtta caagctgctg ttctgatttt
    10861 ccttctttag caagaatggc aagctgtaaa tataagcgaa attgccccaa cataatagca
    10921 attaatttaa tatcatcttc ccctgataag cgtaaatcat gaatcaaatc tctagccaca
    10981 tcaactttac ctcctaggac aagtctggtt aagtcaaaaa tattatcttg taaactttta
    11041 ggaatggctt gctcaatatc agttaggcta atatttcccg tttttttata ggcttttaaa
    11101 aaagccatgt ttttcatgat ttgactaaaa tcatcgtttg attttaaaag cagttgttca
    11161 aaagcgccac tctcaaatcc caaacccagt tgataactgt atttttgaaa ataagttctt
    11221 agctctgctt ctttcagagg gttggcttct aaaacaaggg catcacgttt caaaagctta
    11281 acaagccgtc tcttactatc caatttacct ggagcaaaga taattagtcg agtagtctct
    11341 aagggatttt ctaaataggc ttcaaaggcc tttaggtctt tttcttttaa gaaacttttt
    11401 ttattggtcg tgatatctaa caaatggtca aaaatgacca ccttctgctc agcaaagaag
    11461 ggtaggctca ctagatccat ttctgcatcc tgataagcgg cctcagacat atcaaagtaa
    11521 gaataggcca aatcatcctt atcaaaagca atctgctcca ttaagcggga tttcaactgg
    11581 ctatactgac caatgtcatc tcctgtgaca agggttataa gacccaaatt ttctttactc
    11641 agtttttcaa tcttttctat cgcaatcatg cttttattat atcatctttc tcctaaaagg
    11701 aaagcgactg cttgtttctt cgtcaggctg aaattttaac taacggaccg tttcaagatg
    11761 ccagcttgtc caaccagtca aacgaatagc tccttgtgta tccgttcggt aataagtaat
    11821 ttgtctgtct tccaaacggg ctaaggtttc tctgtgagga tgctgataac ggttattttt
    11881 tccagcagaa ataaaggcaa ctttaggctg aatatggtct aaaaaagctg cactagaaga
    11941 ggtatttgat ccatggtgac ctgcttttaa ataatctact cttagttgag gataacgttt
    12001 tatgatctcg ttttccccct ctttttccaa gtcacctgtg aaaagaaaag ttcgatttaa
    12061 gagcctgcca taaagtacca aagaatcgtt atttttcccg tcccctaact gccaagggta
    12121 gagtacctgt aagacacttc ccataatagg caactggtct ccagctgcta aaaccctaac
    12181 gtgacatttt aaacgcctta gccgcctaac aaagattgga tggcttaaac tgccttgact
    12241 agtcaaaatc tcctttatcc gaatagcttt ggcaaccact tcgatatccc ccatatgatc
    12301 agtatctgcg tgagttacta atagttggtc aattgtgtga atacctctac tttttaagta
    12361 aggaattaag gttttctgag cattagctac gtgatattct cgtcgccagt gctcttttga
    12421 agaaaagaaa gggcgcccac ctacatctat taagagattt ttccctgtcc actccctcac
    12481 caaaatacta tctccctggc caatgtcgat aaaggtcact tcattggtca aaggatattt
    12541 gacagacaaa agcgtcaacg ctataaccaa accgcaagtg ataactcgtt gtcgcacctg
    12601 ccgataatca taaaaaatag cgaagctaat caccaagata agcaagtgcc aactggtcgg
    12661 gcttccaaaa acaagggaac tattgaaagt atttccgaga aattgaatga ctttttctaa
    12721 taaaataaaa agatggttac atattgagac agtcaccaaa ggggatagac aaaaaaccag
    12781 acaaagaagg ggcaaaataa aaaggtcaaa gagataagac aagagccctg ttaagaccat
    12841 tgacatgggg ttaaaactgg aaaaataaaa gagcaaaaat ggtaatattc ctagggaaat
    12901 ggtcaagact tgtaccaact gtcttttagc tcctgataat tcttcaacag taaccactgt
    12961 taacaaaaaa gcatagctaa acgttaatac gccaccaaca gtcattaaga aatgagcatc
    13021 ccataaaaag acaagtaaaa aagtacaagc cagattgtct aatcctttta ttcctaaatg
    13081 acgcaactgc gactgcacga ggcttcgaat aacagaaata ctataacctg tcaacgcggc
    13141 ataaaaggaa gcaaaaggaa gctctatcca ttttatccat tccaaaggaa ctgcgagtaa
    13201 tagcaacacc cttctaaaac aagtgagaaa gaagccaacc tgcatccctg ataaagcaaa
    13261 gagatgaatg atacccaact gactataata atctgtcatc tcaccaaacg acttatctaa
    13321 ataaccaaaa aggaggcccg tcaggtagtg agacataggt cttggaaaat gctgctggca
    13381 atgaacaatg gctcgccgtc ttaaagagga caaatagtca catattgact cgggtgaaat
    13441 cacttcaagc tgttctattt gctctacctt ccctatcctg taaattcctt gataagccaa
    13501 aaaagtctga taattaaagc ctttaaaatt tcgaacttcc tcagcttttt ccaatgtcac
    13561 ttttgcgtgc atcactaacc aacggtgctc tttcttgaac aattgggctt ctgcttggct
    13621 tttcaaacgg taaaataact gataagaatg tttcccatga cgtccaagaa cagctaattg
    13681 atcaccatta atccgaatac tatcaggaac taatgctaca gaggttattt gtactggctg
    13741 aatttccagt ttttgaacca actgctgttt ctgatacagc aaaaacaaag cacagaaact
    13801 aaggatggca aagacaccga gaaattcttt tttagacaaa cgcttgacaa gtaagcaaat
    13861 caaactaagg ctaaggagaa acgttaacca gctagggcta tggatttgat aaaagaaaac
    13921 caagattaaa aaagcaaatt gaatctttga caggggcact agcttagtcc aagaaaatgt
    13981 catcttttaa tttctcgagc gttttttcgc caatccccga aacctgacgg agatcttcta
    14041 acgctttaaa accacctaac ttatctctca tatcaataat atcctgagct cttttagcac
    14101 caatacctgg gatatgttgt agttcttcta agctggcctt attaatatga acctttgact
    14161 gatccttgct ggcagtttct tttttaccag aaactaatga tcttggtagc acagaaatct
    14221 cttctccttg tttaggtaca taaacaactt gctcatcagt tagtttttct gcaaaattaa
    14281 tagcatgttt atcagcttct gaagttaggc ctccagctag ttcaatgaca tctcgcacac
    14341 gactgctagc agttagctta taaactccct ctttttgtac ggcgcctttt aaatccacta
    14401 aaatactgct atcttcttga atttcctttt tttcttcact aacctgctgt ttcacaggcg
    14461 atatttctct caaagcagga acggctgcct tgctctgcct atttccccag aacaaaaaac
    14521 tgaagcttag tattagaaaa agtcctaata aaagactaat taggtatggc agaaaatacc
    14581 gctctctcaa aaacgatttt cctttaatta ataattcgtc taacattttt ttacctcatc
    14641 tttttattcg taaatgatat ccaaaagtat agtagtattg tcattttttg tgcttaaaag
    14701 agttaagggt atcatcgaat atctttttta gaaatgaaaa aagtgattaa gcattagcgg
    14761 cttctttcaa tagaagacta atctcaatca cttcttattt cagacacatg ttagccctca
    14821 tgttcttaat ggcgatgttt atctggattc caaacaaaac tagcaatata ggaaaataac
    14881 atggttaata ataataccac caccaaaacc aatcctaatg gtaaacggta gagataagtt
    14941 aaagggttcc ttgctttgcc tggttggaaa ggtgccaact cgtcatccat ccgatcaaac
    15001 tctgcctgaa tacggtttgc cacctcagca atcccttcat cattcatgcg tttaatatct
    15061 gagacgtcaa tgggattccc aaaagtcatg tcaacccgtt ctcccgctag taatccctta
    15121 accgacatag gcccctgata ggctgctggc ataatcttga ccttagccat tttagcaatc
    15181 acagcgaccc ctcctttgac ctcttgcgaa tgcctacttc cacttggaaa cataagaaga
    15241 gaacgattgc tctttttgag catattaaca gggtaacgaa tagcatctgg actcggtttg
    15301 tcacgatcaa taggaaaagc accacacatt ttaatccacc aagcaaagag acgatttgca
    15361 aacaactctt ttttagccat aaaaataaac tgcttaggac gtgctgcaaa tgccatgtaa
    15421 actggatccc aaaaggttcg atggggtgct accagaatat aattctcaga agcatctaac
    15481 atcttttctt catgatggta gtgggcattg ccattgacaa tccataataa aaagactact
    15541 aaaccgcgta agtaagcgta aaacacaggc ttctccttta atagaataat cttacttatt
    15601 gtatcatgaa agttgacttt tcgaaagtca aaatcactct tcaaacattc acctcaagcc
    15661 tattttatgt taaaataaca ggtatgataa aagcaatctt aaaagaaggc gaacgtattg
    15721 atcaactctt ttcaagtgat gttggcatta ttcaaaacaa ggatgtcttt agttactcta
    15781 ttgatagcgt cctcttatcc cgcttcccca agatgccttc caaagggtta attgtggatt
    15841 tgtgctcagg aaatggggcg gttggcttat ttgctagcac tagaaccaag gcagccatcg
    15901 ttgaagtaga attacaagaa cggcttgctg atatgggaca acgctctatc caactcaatc
    15961 aactggaaga ccaagtaacc atgatttgtg atgatttaaa aaacctccta aaccatgtgc
    16021 cacgttcagg agttgatttg atgctctgca atccccctta ctttaaaagt cacgaaagtt
    16081 ctaagaagaa cgtctctgaa cattatttac tggctcgtca tgaagtgacc actaatttag
    16141 aagagatttg tcaggtagcc cgtcatgccc ttaaatcaaa tggtcgtctg gcaatggttc
    16201 atcgaccaga tcgattcttg gaaattatag atagccttag agctaatggt cttgccccta
    16261 aaagggtaca atttgtctat cctaaattag gtaaatcagc taatatgtta cttattgaag
    16321 caatcaaaga cggttctatt gaagggatga ctattttacc tcctttagtt gtccacaagg
    16381 aaaacggaga atacactgat catatttttg agatttattt tggtgctgct tccaaaggaa
    16441 aacctaatca tgaccactaa aaaagcttat atgtatgtgt tagaatgtgc agataaaacc
    16501 ctctacactg gttatacaac tgatttgaaa aaacgcttag ctacacataa cgcaggaaag
    16561 ggggctaaat atactcgcta ccggttacct gtttctttac tttattacga agtgtttgat
    16621 agcaaagagg ctgcgatgag cgctgaagca ctctttaaaa aacgaaaaac aagaagccag
    16681 aaattggcct atattgctac tcatcaaaaa gaaaagaaaa atcattgaga cttatcatct
    16741 caatgatttt tttatgcttc actgtcttcc acaggcacca attctttgat tggtaacttc
    16801 aagacatctg atagaatcaa gggaaccact tcaagggtta cttcggaata ttgaagacca
    16861 aaccaccgca ttggactggc agtgacatgt ttgatgctcg ctttagtctg cataataaag
    16921 cgttcaacat tagtgagttg acgtacattg gagacccgct cttctattaa aacaaagcga
    16981 aaatcaccaa catcacggcc aggtgtgatg gtatattctt gttcttgctt tggcaaacga
    17041 ccactttcca tcaagtcatg aacaatcgta cgcaaatagc gtggcacagt ctgtggcatt
    17101 ctaaaaccga gataaagttc gactcgaacc atataatctg ttcccatcat atccacctta
    17161 tacttggctg tataaggctt atcagttacc tgaacattca caaaccaata aacttgagcg
    17221 cgcttgggcc gcttatctaa aatggaatac aaaatagaac ggtcaatcat gtgatcctgc
    17281 atgcgattgg agagataaac cacattggtt tggtaaaggt caaaacgaac gtcgtcacga
    17341 agttgcttaa tctgctcctt gtaatcattc aaattgagag atttcacata cttgaaaaca
    17401 atgcgtgtgc ccgcatgcca aataaacatg acaaagacaa ttgctagcgc taaaataacc
    17461 actacatagc caccgtgcat gaatttgaca gcggaggccc agaagaaaat gaattctaca
    17521 agggcaaaga aagtcatcac taaatgagct aggaaaggtt ttacaccttc tttaatcaaa
    17581 tagtaattca ataatatagt ggtcattaac atggtaatgg taatggccag gccataggct
    17641 gcttccatat gagcagaggt tctaaaatac aaaaccgtac aggacgtgac tgcaaagaga
    17701 atccagttaa tcaccggaat atacaattgc cccaaattag caccaggata agtcactcga
    17761 aatagaggga atattttcaa gcgcatggct tctgaaacca aagtaaaaga acctgaaatt
    17821 aaggcttgtg aggcaatgat ggctgctaag gtggcaagaa taaccacata caccgttaat
    17881 tgggaaggta cactagcaaa gaaagggttt agttcaatac ctgagtgttt attcgctaaa
    17941 atccacgcag cctggccaca gtaagacagc acaatacaca tcttaacaaa gggccagctc
    18001 acataaatat ttcctcgacc aacatgtcct aaatccgaat aaagggcttc agctcctgtt
    18061 gtcgcaagaa aaatggaccc caagataaaa atcccacgat ggttttcagg actaaacagc
    18121 aaatgcagag catagtaagg attgatagcc ttgaaaattt ccagatgccc caacatatta
    18181 aaaaaaccac taacacctag aaaactaaac cagataaaca taactggtcc aaaaatttta
    18241 ccaataaaac ctgttccaaa ccgctgaata ccaaacaaga ctattaaaat cactagagtt
    18301 gtgataatga cattggtttg attttggtag atgtgactca gaccaggaac ggctttcaag
    18361 ccctcaatag ctgaagtaac tgttaccgca ggtgtaagag ccccatcaga caaaagtgtt
    18421 gcacctccaa tcatcgccgg aataatgagc cacggtgaca ttttacgtac caaggtgaat
    18481 aaagaaaaaa tcccaccttc atggtggtta tccgctttta gagcaatcaa aacgtacttg
    18541 attgttgtga ttaaggtcag tgtccaaata attaaagaga ttgatcctaa aataaaggat
    18601 tcagaaacct gattaacccc tccttgattt tcaactagag actgcatcgt ataaaggggg
    18661 cttgttccaa tatctccata aacaatacct aaagcaataa taaaacctgc ttttgaagct
    18721 ttgtcaaagg cagtaagatg gctatcagac atcttatact cctcctaatt ttttaaaaat
    18781 tataacaaaa taactgaaac cattataaca gattccatta taaaagcaac tttttcagaa
    18841 aattaaaaag agctgactct catcaactct ctttaagctt attcaccttt gtgacgaatc
    18901 acaaaaccag tatttttagc acttgatttt ttctctttat tgaaaaagtc tttactatta
    18961 cgttttgatt tacgtttgaa atcgcggttg ccactgccat cgtgaccatc acgcttatca
    19021 cgtttttgac gacgacggtc accatcacgc tcatctctgt tacggtcacc acgatagccg
    19081 ccacggcgat caccacggtt acggttgtca cgtccacgac cacctttgcc atttttattg
    19141 ccgtgaccac cacccacata tttgaatggt aatggttttt cacgtgcaat ttcaacttcc
    19201 ggtaagctat ctggatcttg aactgttaag ctcaaaatat ataaagctaa ttcttcagga
    19261 gtgaattcgg cagccaattg gacagcatcg cctttgaatt tatcaaagtt tgaacgaata
    19321 gtctcatccg caaagtcgcg ttcaattttc ttgagggcta cttttttctt ggcttgaaag
    19381 gcctcttcag cagttgctgg gcgaagaggt ttcatctgtt ttttcgttaa gttctcaatc
    19441 attgagaggt agcccatctc atttggtgag acaaaagtga tagactcacc agatttaccg
    19501 gcacgacctg tacgaccaat acggtgaaca taactttctg gatcttgggt aatatcgtag
    19561 ttataaacgt gggtaactcc tgaaatatca aggccacgcg ctgctacatc agtggcaaca
    19621 agaatatcaa tttggtcgtt tttgaaatca cggataacac gtaagcgttt attttggtca
    19681 agatcaccat ggattccttc ggcacggaaa ccacgaagtt tcaagccacg tgtaatttca
    19741 tcaactcggc gcttagtccg cccaaaaacg atggataatt ctggctggtt aacatccatc
    19801 agacgggtca tagtatcaaa tttttcttgt tctttcacac gaacatagta ttgatcaaca
    19861 ttgacgtttg ttaattcttt gttcttaatt tgaacatgtt cagggtcttt catgaattta
    19921 accccgattt gtttaatagg tgccggcatc gttgcggaga agagcaaggt ttgacggtct
    19981 gctgggacac gactaataat agcttcaatg tcttccaaga atcccatgtt aagcatttca
    20041 tctgcttcat caaggattaa ggtttccaca tgatctaaaa taagagcctt acgcttaatc
    20101 aaatctaaca agcgacccgg tgttcccacg acaatgtggg cacctgattt aagggctttg
    20161 atttgttttt caatacttga tccaccgtaa acagagcgaa ccttaacccc tttttcacgt
    20221 ccaaaacgga acaattcttc ctgactttgt acagccaatt cacgcgttgg tgcaataacc
    20281 aatgcttgga tgatattttc gttagtacga atcttattca aggttggtag accaaacgct
    20341 gctgttttac cagttcctgt ttgtgcttga ccaataacat ccttcccttc tagtgcgaga
    20401 ggaatcgtca tttcctggat aggagatgct ttctcaaaac cagcggtaac aacagctgat
    20461 tggatgtctt gtgataagtt aaattctgta aatttcaatt gtttctcttt tctaaaaagg
    20521 cagtgcgaag ctgccttata aaccttttaa ttttcgtcgt ttgattaaca acctaactag
    20581 tgtaacacaa ttagaatcaa aaagatagga aataaggcta atataagcaa taacacgctt
    20641 tcacgattat ccgctaaatc ataaatatgc ttacagttgt gaagatatcg ttttttgata
    20701 gaatcttaaa tctgcaaaag agataactag ccactcattt cttttaaaaa gttactcagt
    20761 aaattgatct ccaaaacgtt agactgttga aaatcatgtt tcggttacat tttctcttct
    20821 aaagtgacat ctgggtattt gtctgcaaac cagcgaaggg caaaatcatt ttcaaatagg
    20881 aaaactggtt ggtcaaaacg atccttagct aaaatgtttc ggcttgagga catgcgttga
    20941 tccaaatcat cttctgaaat ccagcgaaca gtctttttac ccattggtgt catcacaact
    21001 tcagcgttgt attcaccttc catacgatgt ttaaagactt caaactgcag ttgacccaca
    21061 gcacccagca tgtattcgcc tgtctgataa ttcttataaa gctgaattgc tccttcttgt
    21121 actaattgtt caataccttt gtggaaggat ttctgcttca taacattttt cggagaaacc
    21181 ttcatgaaga tttctggggt aaaagttggt aagggttcaa attcaaattt atttttccca
    21241 actgttaagg tatccccaac ttgataagtt cctgtatcat aaaccccgat aatatcccca
    21301 gcaacagcat tcgtcacatt ttcccgtgat tctgccataa attgagtaac attggagagc
    21361 ttagcgcctt tgctagtgcg tgtcaaatta actcccatac cgcgctcaaa ttcaccagaa
    21421 acaatacgca caaaagcaat acggtcacgg tgtttagggt ccatgttagc ttgaatttta
    21481 aagacaaaac ctgaaaaatc tttagttaag gggtcaacaa cattaccttc tgtagtcttg
    21541 tgaccatgag gttctggggc aaactccaag aaagtatcaa gaaaagtctg aactccaaag
    21601 ttcgtaaggg cagagccaaa aaagactgga gttaggtcac catcaagaat agcttgttct
    21661 gaaaagtcat tacctgcttc ttgaagaagt tcaatatctt ctttgacttg ttcataaaag
    21721 ggattattgg caaacagctg atcaccgtct tcaatacttg caaaacgctc gtctccctta
    21781 tagagttcta aacgtttatt atgtaaatca tataagccct caaaggcgcg tcccatacca
    21841 atcggccagt tcatcggata agaagcaatc cctaaaactt cttctaattc ttccaagagt
    21901 tctaaaggct cacgtccatc acggtccaat ttattaataa acgtaaaaac cggaatatta
    21961 cgatgcttaa caacttcaaa aagttttttc gtttgcgcct caataccttt agccgaatct
    22021 acaaccatga cagccgcatc cacagccatt aaggtacggt atgtatcttc cgagaaatcc
    22081 tcatgtccag gtgtatccaa aatattaaca cgcttacccg cgtaatcgaa ttgcataaca
    22141 gatgaagtaa cagagatacc acgttgcttt tcaatatcca tccagtcgga ctttgcaaac
    22201 gtacctgatt ttttcccttt tactgtccct gcttctctaa tttcaccacc aaaatataat
    22261 agttgctcag taatagtcgt cttaccagca tccgggtgag agatgatggc aaaagtacgg
    22321 cgttttttaa tttcttctgt aagtgacatt cttttttcct tgtatagtaa aatggcagag
    22381 cttttctctg agttattttc gttaggtatt atttgtttat caaaagagtt gggctacatt
    22441 gggtttctcc atatacgatt agcctctaaa gttcttcaaa actggtatta taacttatct
    22501 tgggctatca atgagtgtct tttttaaaaa aatcgagcct ggaaatcatc ccagactctt
    22561 cttattatag ctgattttaa gtgctttttc aacgacctac ttttctttag agaaagcaaa
    22621 tgccagctgt tgagcttcac caaatttaag gtaaaaatat tcggttattt taaccataaa
    22681 agtcaagcca cttgtaacga tcacaaaatt aagtagcaaa tgacgcgtat gaatcactgg
    22741 aatatccatc acaaaaccat aatttccttt cgtggctaaa ctgaccaaca gaagaagaaa
    22801 gttaacagtt gctaaatagc gtactactga tagcagtctt aactgactgg catcgtaaaa
    22861 acgcaagagg taaatcaaac cattcactaa caaagcataa tgaccaagat aaaacgagac
    22921 atttgctaca tgccataatc taaacggata aagatctggc gataaaagtg caaggaatgt
    22981 tccacctatc cccaaaacca taaataattg cttaaacttg ttacgatcag gtagaaaaaa
    23041 gatagctaac atagcgatgc gacaatgata caacggcaaa gcttcatcta aaggaaaccc
    23101 ccttaacacg taccatgtgt acaagccaat cacctgagat aactgtaaaa acaagaaaaa
    23161 ataacgataa gattttaacc tatatgcttg aaaagtcaaa aaaacaagca aaagcgctat
    23221 caagagtgac aacaagtaaa agataagtga gatatgaggc agtccgatgg ggtcaattgc
    23281 aaaaaaatcc ataatcgtcc ctttctatat tttttcatta taccttaatc ttcatgagag
    23341 aacaagccct tctctgaaag ttttataaaa atcaaggctg atagctgtca caattcttgt
    23401 cagtcactaa tcgatctact aacttttcta gcgacatcga gtggctgcct tttaaaagaa
    23461 tttgatcaaa gggattaaga atgttttgaa catattgtgc catggcttca aactggtctt
    23521 cttgttcagt ttttaaaaaa taatgtactt gctcagcagg ataaacctga cttgccaacc
    23581 gtgctaattc tttaatatga tccccataaa aaacaagctg atcaatatta cctgatgtta
    23641 agctgtcaat taactgagaa tgaagtatca ctgaatcctt tccaagttct ttcatatctg
    23701 ctagcactgc aatctttttt cctccggggt tctttgctat gttagcaaat gtttctaaaa
    23761 tcagacgcat ggctgttggg ttggcattgt agacatctga taaaatatca gcaccattgg
    23821 ctgctttttt ccactccgta cgattacctg tcaacgtcac agtctgtaaa gcagctataa
    23881 tatcctcgtc tgttactgcc aatagttttc ctacataggc tgctaccata gcattggtcg
    23941 cgttgtattt tcctggtaga ggtagggaca caggtgttgc taacacattt gttgtaaacg
    24001 ttaagctatc tttgtcttct tgaataccag taacgtcaat ttcctgctga tttccaaaac
    24061 ggataaccat ctgattttct ggaagatatg ggtcaataat cggatctccc ggagcaatta
    24121 aaatcccatc tgaattcatg ccgtcaacaa tctgcatttt gccttgggct attttgtctc
    24181 ggcttccaaa atattctaaa tgagcttcgc cgactaaggt taaaactgca atacgaggcc
    24241 ttgcaatttc agacagaaga caaatatctc ccatgtgatc ttgtcccatt tcaagaacaa
    24301 ttttttccgt atcgtcaggc atgtggagaa cggtgtaagg caaaccaatc tcattattgt
    24361 aatttccttg cgttttgtaa gttttgtaag tggttgatag gacagctcct atcatatctt
    24421 tggtggaagt tttaccattt gagcctgtca cagcaataac atccacgcgc atcttatcaa
    24481 tataataatg ggctagcttt tgaaaagctt ttaggcagtc ttcaacaaga aggtgaggtt
    24541 tccccggcaa ttctttttct gaaaaagtag cgacagctcc attttgaaag gctaaatcaa
    24601 taaactcatg accatccctt tgacctttta agggtaaaaa gaggtctcct tttgtaatct
    24661 ttcggctatc aaattcaatg tgatgcagag gaacgtcgtc taagtcagac acattatttt
    24721 gggcgtcaac tattttagcc acttcgtgta aggttaattt cattttgttt tctttctaag
    24781 aacactagta tttacgatgt cctatctatt atagcacaaa acattagaaa aatttctaaa
    24841 atgtcaaact cccttaatgg ctctactata aagagcaaca aaaaaaggac gaacgtcaca
    24901 tcttgacgat gtttgccgtt ccatccaagt caatgtttat tcatctcata ggtttaaatc
    24961 aggtggctct cacgctggtc aaacatttct tgtgccagtg tcaccagctc ttcaatcaaa
    25021 tctggataag ccaatcccat attttcccat aagagtggat acatagacca ttgggtaaag
    25081 cctggcatgg tgtttaattc gttaaggtaa acctgtccgt cttgggtcaa gaaaaagtca
    25141 caacgggaca acccacaacc acctaaggct ttaaaggcta cttcagcata agaacgcatt
    25201 tctgtcacaa tactctggtc tacatctgct ggaatggcca tcgtaatctt attgtcaaca
    25261 tattttgctt ggtagtcgta aaaatcaacg tccttgatta cttctcctgg tagagttgat
    25321 ttaaccttat catttccaag gagaccaact tcaatttctc tggcaaccac gccttgttca
    25381 atcaagacac gactgtcata ggtcagggct aactgaatcg cttttcttaa ttctactttt
    25441 gtttgggctt tggaaatccc cacagaagaa cccatattag ctggttttac gaagatagga
    25501 aaagtaagtc ttgctaaagt ttcaactaaa caagcctcca aatcttgtcc gtcaatataa
    25561 acagtatagg caacttgtgg aattccaatt gattctaaga cacgtttagt agtaatttta
    25621 tccatagcta tgctagaaga catgacatta gtccctatat atggcattct aagcacttct
    25681 aagaaacctt ggatagagcc atcttctccc ataggtccat gtaatacagg aaatacaact
    25741 gcaccttctt catagatatc acttggtttt attttttgag ttagctctat cgtctcattt
    25801 gtcatcaagc gttctgattc cgatggcttt tctgaaaatt gctgagtttt gataaattgg
    25861 cccatctgag taataaaata agtttttact aagaacttat catagttgac tgctcgcatc
    25921 acactctctg ctgaaagcac tgagacttca cgttctgctg agcggcctcc gtataataga
    25981 actaacgttt gtttggacat agctaacctt tcttatctga attgaatgtt tttttattat
    26041 aacaaaaaga tgtagccttt tcaaaacctc tctttgcgag taaaggcacc aacatgatca
    26101 ttaaagttca gtacgattct caatagctct caataatgtc acttcatcag catactcaat
    26161 atctgaacct actgcgagac ctcttgccaa acgagttacc ttaatcccgg ctggtttcaa
    26221 gacacgtgaa atatacatgg acgttgcttc cccatctgct gtggcattgg tagctacgat
    26281 aacttcgctc accttaccat ccattagacg ggtaattaaa cttttaaggt tgatgtcatc
    26341 tggccccaca ccattcatag gcgaaatcaa gccgtgaagc acatgataat agccatgata
    26401 ctcttggatt ttttccatgg cagaaacatc tttagcatct tctactacca gaatggtcgt
    26461 ctgatcacga ctcgtgtctg tgcaaatgtg acaaggatcg tcatcggtaa ggtttccaca
    26521 aatcgaacaa taggtcagtt ctcttttagc tgctaataag tttttagcaa aatcattgac
    26581 atcttcattt gacattccaa tagtataaaa ggctaatctc gtcgccgtct tgataccaat
    26641 tcctggaagt ttagagtaac tgtcaattaa ctttgcaatg ggtgttggat aaagcactaa
    26701 gttctccttt attgttttcc atatatcgcc acataaagcg ccctcacatc tttgccgata
    26761 tattcgtaat atgtggatac ataacagcaa ccgcaatatt atcttcgtta tttgtattat
    26821 aagctactac atttaagttg taagttacta aggtctgatc tgaattgtct gtgatacaag
    26881 tctcagcagt tccaatttta gcgtgaatcg tgacagaacc gtttggatta gccaagtaaa
    26941 aactttttta ctcgacttgt taccttggcc ttgggatatt tggcttttgt tttttgattc
    27001 atgttacttt tttagaaacc gcaaaccatc cttatgatat aagtttatag gaattgtttc
    27061 attccatgca gaaatgatgt ttactcaatt gactatgcta ttttctcaac aatagtgcat
    27121 aaagcaaact catggttatc tttaaacttt catgctacac tttcttcact aaaagtgtat
    27181 taccaaaagc attattaaga aatctctaag cacaaaccat aagatagttt gtacgcctga
    27241 tgaatctgtt gctgcatcac tgtccacttt ttaccctatc aaggctattt ttgaaagaca
    27301 actaatgatc tgcattattt tgggaactac tcccatagca aattcttttt acggattttt
    27361 tgggaatact ccttttttag ggaataaact aaccattatt tagctaaatt ttttaaactt
    27421 cgcttcttat tttaccataa attataaaaa gaagactgag aataggacgc cctcagtcta
    27481 aaaaaatatt ttctcattga gttttactct tttgcggatg atagtacact ttaaaatata
    27541 aaaaactaac aaaaatggct ccaccgataa tattaccact atagacaaaa ataaaattag
    27601 tggcaaaatc caaccatgtt gctcctcctt caaaaatagc agcaggaatg acaaaagcat
    27661 ttgctacact atgctgaaaa ccaagggcta caaaggtcat gacaggaaac caagtaccaa
    27721 ggaatttacc agcagcatca tttgccccat agcatagcca caaggccaaa ccaacaaacc
    27781 agttacaacc aattcctgat actagtgcct gcaaagggct cgctgctatt ttagcatggg
    27841 ccacctcaat cacttcttct ttaaaaatac cagctgatgt caaacctaga aaatgaccaa
    27901 acacaaaagc tacaaaaaca gctccaatca cattaaacaa ggtaatgata agccaattct
    27961 tggcaagctc gctaaactta atttttttgg ccaacaaagc tgcagaaaca gccatcatgt
    28021 tccctgtaat taattctcca cccgccatta aaataataat tagaccaatc ggaaaagcac
    28081 aagctcctac gatactggaa aaagcaccaa aagtttctaa accactggcg gcaattctca
    28141 cgtagagtaa ataaccaagg ctaatcatcg ctccaccaat aaagcctaag atagatttag
    28201 ccaaaaaagt tttggtaacc ttatgttccc caatatgaat agttgcttct aaaatctgtt
    28261 ctggtgtttt catgatatct ccttttgtta ctgtattcac aaacacacac tttagtatat
    28321 caattttcct aaaaaatgca agccctttca acaagaaagg gctcgtcatt atcatataac
    28381 tatctctatt ttagtcttag gcatttatgg tgggaagttt taagtttttt gctaaaaaac
    28441 gccatagaac taaagcaaga tagaaatcca atatgccatg aataagcgaa cctaagccaa
    28501 tcaagcctag catagaccaa aaataagaca tagaagttgc tgtggttgct gttaaaatat
    28561 agacaactaa aaattcagcc aagccatgaa tgacattaat gaaaaacgca aaactaaaac
    28621 attttactgc cgatgtcatc agttgtggtt ttctacttaa ccaccacgct gccaaaatag
    28681 caaacaataa atgagagagt gctcttaaaa caataattaa aggaagtcca gctaataaaa
    28741 aacctaatgt ggtaccaaga gcgaccaaaa ttgctactgg aatcgacata aaaatagcca
    28801 aaaacaaagg aacatggctc gccagagtaa aggaagctgg tccaataata agcttaagag
    28861 gcatgattat cggaatcaaa atcgcaaatg cgaccaaaat agaggtataa gctattagtt
    28921 gtgttggttt tttagtcatt ttatctcctt ttcaaaaata gtatacctta aaaaccaata
    28981 actatcaaga cacctgtatc ttttggtatt tttaaacgat accttgttgc tgataaataa
    29041 tcaatagaat attttagcat ggtattcata acaaccatca atggcataat atactgtagt
    29101 tatattcctt tgtataagat acctgctaat tctaaatcat gcttgatagc taaaaaggct
    29161 tcttgactag gacacgaaat taagtgacta tgtagtcctt ctgtcaacga agacaataac
    29221 tctgctttag attgactgag tttggacatg aaattagtta catcagagtg tgtcttaata
    29281 tttaaagggg ctgttatcat accgtaaata ggatgttcta cctctaccgt cgtaataatc
    29341 ccttgatgag ctaaaataat ttctaattct tttttagttt cttctacatt atgttggcac
    29401 acaatacgtg cacaaaattg gtgactataa agcgctgttg acataatata accttttgga
    29461 gtcgaaataa tatcaatctg ctgagctcga agaagagcta tatctccaac gattacttgg
    29521 cgactcactc ctaataactt acctaaacga gtagcagata cagctttttg ttcactattt
    29581 aagcactcaa taattttttg acgacgatct tctgctttca taatctatct atcctttttt
    29641 atttctatca tatcatattt ttaagcaggg ttttctttct tcttaggata tagaatcaaa
    29701 aaaccagacc aaagtctggt ttttaagtta ttcaccacca aggtagtatt ctttaacaat
    29761 gttaagtttt tcatctaatt caaaaacaag tggtgggaag tttggaattt ccacatccat
    29821 gatttcgtca tctgaaagac ctttaatgtg tttcacaaga gcgcggattg agttaccatg
    29881 tgcgccaaca aagacgtttt taccgtcaag aagagctgga gcaatttttt cttcccagta
    29941 aggcattgcg cgttcaagag ttacttttaa gttttcagca tctggaataa gggctgggtc
    30001 aagatcagca taacgacggt ctttgtgtgc tgaatactca tcatctttag ccatagctgg
    30061 cggcaatacg tcgtaagaac gacgccagat atgaacttgt tcatcaccaa attgttctgc
    30121 agcttcagct ttgtttttgc cagtcaaagc accatagtga cgctcgttca agcgccatga
    30181 tttttcagtt ggaacccaca attgacctgc attttcaagg gcaaggttag ttgttttgat
    30241 agcacgtgtc aatactgaag tgaaagcaag gtcaaattca atacctgctt ctttgatcaa
    30301 tttacctgca tcaatcgctt gttgtgtccc tttttctgaa agatcaacat ctgcccaacc
    30361 agtgaagagg ttagctttgt tccattctga ctcaccgtgg cgagcgaaaa ccaattttac
    30421 catgagttaa tatctccttt taatt
//
LOCUS       DATURV010000015        29478 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DATURV010000015 DATURV010000000
VERSION     DATURV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29478
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.015"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..289
                     /gene="gpsB"
                     /locus_tag="VCF75_000662"
     CDS             <1..289
                     /gene="gpsB"
                     /locus_tag="VCF75_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEP2546246.1"
                     /translation="QEFKTSMRGFDKKEVDEFLDNVIKDYENFNAQIEALKAENEALK
                     KAKFQARNTVSATVQQPVPQPTRIAQSATNFDILKRISKLEKEVFGKQIIE"
     gene            303..671
                     /gene="rnpB"
                     /locus_tag="VCF75_000663"
     ncRNA           303..671
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VCF75_000663"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            734..1888
                     /locus_tag="VCF75_000664"
     CDS             734..1888
                     /locus_tag="VCF75_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEP2546247.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWENYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKINGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            1902..3371
                     /locus_tag="VCF75_000665"
     CDS             1902..3371
                     /locus_tag="VCF75_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546248.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQITKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGNQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(3516..3998)
                     /locus_tag="VCF75_000666"
     CDS             complement(3516..3998)
                     /locus_tag="VCF75_000666"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEP2546249.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGTTWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(4072..5403)
                     /locus_tag="VCF75_000667"
     CDS             complement(4072..5403)
                     /locus_tag="VCF75_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEP2546250.1"
                     /translation="MEIIGSLGVLVGVIVIIYLSVKEVNIIIAAPLATSLVILFNQMD
                     PTKTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGATTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMAMG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIVIAFDLFS"
     gene            complement(5474..6253)
                     /locus_tag="VCF75_000668"
     CDS             complement(5474..6253)
                     /locus_tag="VCF75_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEP2546251.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETLLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYIAQ"
     gene            complement(6286..6945)
                     /locus_tag="VCF75_000669"
     CDS             complement(6286..6945)
                     /locus_tag="VCF75_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEP2546252.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(6947..7598)
                     /locus_tag="VCF75_000670"
                     /pseudo
     CDS             complement(6947..7598)
                     /locus_tag="VCF75_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
     gene            complement(7622..8809)
                     /locus_tag="VCF75_000671"
     CDS             complement(7622..8809)
                     /locus_tag="VCF75_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP2546253.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALGNHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            9008..9904
                     /locus_tag="VCF75_000672"
     CDS             9008..9904
                     /locus_tag="VCF75_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP2546254.1"
                     /translation="MDIKQIRYFIAIVENHFNLNQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSINIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNLKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQFKDIKCIPMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
     gene            10034..11641
                     /locus_tag="VCF75_000673"
     CDS             10034..11641
                     /locus_tag="VCF75_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEP2546255.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            11751..12386
                     /gene="gmk"
                     /locus_tag="VCF75_000674"
     CDS             11751..12386
                     /gene="gmk"
                     /locus_tag="VCF75_000674"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEP2546256.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKKTFKAK"
     gene            12402..12719
                     /locus_tag="VCF75_000675"
     CDS             12402..12719
                     /locus_tag="VCF75_000675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEP2546257.1"
                     /translation="MMLKPSIDTLLDKVSSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            12784..15168
                     /locus_tag="VCF75_000676"
     CDS             12784..15168
                     /locus_tag="VCF75_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEP2546258.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            15230..16165
                     /locus_tag="VCF75_000677"
     CDS             15230..16165
                     /locus_tag="VCF75_000677"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEP2546259.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTILLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSD
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGA
                     LSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR"
     gene            16155..17477
                     /gene="rsmB"
                     /locus_tag="VCF75_000678"
     CDS             16155..17477
                     /gene="rsmB"
                     /locus_tag="VCF75_000678"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEP2546260.1"
                     /translation="MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLTYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            17515..18255
                     /locus_tag="VCF75_000679"
     CDS             17515..18255
                     /locus_tag="VCF75_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEP2546261.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            18252..20150
                     /gene="pknB"
                     /locus_tag="VCF75_000680"
     CDS             18252..20150
                     /gene="pknB"
                     /locus_tag="VCF75_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEP2546262.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSREHKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGELSLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            20273..20965
                     /locus_tag="VCF75_000681"
     CDS             20273..20965
                     /locus_tag="VCF75_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEP2546263.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            20962..21966
                     /locus_tag="VCF75_000682"
     CDS             20962..21966
                     /locus_tag="VCF75_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2546264.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            21959..22600
                     /locus_tag="VCF75_000683"
     CDS             21959..22600
                     /locus_tag="VCF75_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2546265.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            22637..24037
                     /locus_tag="VCF75_000684"
     CDS             22637..24037
                     /locus_tag="VCF75_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2546266.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPRLQEKADVITKKVEEDGILYALEELGLIDKELQFPQL
                     DLANHKGPKATIKTNHGDMTLVLFPEHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQYFIVQNSKVP
                     YAKKELERGGWPAPIAASYAENGGTPHLDRRHTVFGQLVNETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            24037..24414
                     /locus_tag="VCF75_000685"
     CDS             24037..24414
                     /locus_tag="VCF75_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP2546267.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALDNGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKASLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(24432..25373)
                     /gene="cysK"
                     /locus_tag="VCF75_000686"
     CDS             complement(24432..25373)
                     /gene="cysK"
                     /locus_tag="VCF75_000686"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEP2546268.1"
                     /translation="MTKIYKTITELVGQTPIIKLNRLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETAIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARRTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIETK"
     gene            complement(25501..26133)
                     /locus_tag="VCF75_000687"
     CDS             complement(25501..26133)
                     /locus_tag="VCF75_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEP2546269.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSSMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            26188..27513
                     /locus_tag="VCF75_000688"
     CDS             26188..27513
                     /locus_tag="VCF75_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2546270.1"
                     /translation="MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPSGAYYCRFCLVFGRNQSDKLIYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEGITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLAKKEIKEMNYKGYSHDLSTMSTN"
     gene            27485..28150
                     /locus_tag="VCF75_000689"
     CDS             27485..28150
                     /locus_tag="VCF75_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEP2546271.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACQDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSLGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            28230..28778
                     /gene="raiA"
                     /locus_tag="VCF75_000690"
     CDS             28230..28778
                     /gene="raiA"
                     /locus_tag="VCF75_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEP2546272.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDDATNILYRREDGNLGLIEAK"
BASE COUNT         9565 a         5237 c         5988 g         8688 t
ORIGIN      
        1 gcaagaattt aagactagta tgcgagggtt tgataagaaa gaagttgatg aatttcttga
       61 taatgtaatt aaagattatg aaaatttcaa tgctcaaatt gaagctttaa aagctgagaa
      121 cgaagcctta aaaaaggcga agtttcaagc tagaaataca gtttcagcga ctgttcaaca
      181 accagtaccg caaccgactc gtattgctca atcagctact aattttgaca tcttgaaacg
      241 tattagtaaa ttggaaaaag aggtttttgg taaacaaatt attgagtaat gaaaactgtg
      301 caatttttgg ataatcgcgt agtattttaa tactatgagg aaagtccatg ctagcactgg
      361 ctgtgatgcc agtagtgatt gtgctaggcg aacacataag cctagggatg tgcatacaca
      421 ttacggcgaa ggaaatggct aagtcgtttg atatgccaaa gtacttctga aagtgccaca
      481 gtgacgtagt ttttatggaa acgtaaaaaa tggaacgcgg taaacccctc aagctagcaa
      541 cccaaacttt ggtaggggca tgggatagtt ggaaacgaac aagctatcct gactgtcaac
      601 agacggtaga cagatgatta tcgaaggaaa taattcctag ttatttccgg aacaaaacat
      661 ggcttataga aaattgcata ataggttaag ctagcacttg ctagctttta tttgatttaa
      721 aagaaagaga tatatgaaag aaacatttag attagtcgca actgcagccg caggtttaga
      781 agctgtcgtt ggtaaagaag tcagagcact aggctttgat tgtcaggttg aaaatgggaa
      841 agtttatttt gaaggtgatg tagaagcgat tgttaaaaca aatctttggt tacgtgcagc
      901 agatcgtatc aaaattatcg taggacaatt tcctgctaga acctttgaag aattattcca
      961 aggtgtcttt gccttggatt gggaaaatta tttaccgcta ggagctaaat ttccaatctc
     1021 aaaagccaaa tgtgttaagt ctaaattgca taacgagcca agtgttcagg cgattaccaa
     1081 aaaggccgtt gttaagaaac tgcaaaagca tttccacagg ccagaaggag ttcctcttca
     1141 agaagttggg tcaacgttta acattgaggt atctatttta aaagaccaag ccactattat
     1201 gattgacacg acaggatcta gcctatttaa acgagggtat cgcgttcaaa aaggtggagc
     1261 accgatcaag gaaaatatgg cagctgctat cttggcctta tctaactggt ttccagataa
     1321 acctctggtt gatcccacct gtggatcggg aacgttttgc atcgaggcag ccctgattgg
     1381 gatgaatatt gctccaggat ttaaccgttc gtttgctttt gaagaatgga gctgggtaga
     1441 taaagacatg gtgcaacaag ttcgtgatga tgctgaacaa gaagctaatt atgaaatcga
     1501 attagatatt tctggctttg atatcgatgg ccgcatgatt gagattgcta aaagtaatgc
     1561 tgaagaagct ggtttatctg atgtgattac ctttaagcaa atgcgtctgc aagactttcg
     1621 aacagacaag attaatggtg tagtgatatc aaatcctcct tatggagaac ggttgcttga
     1681 tgacaaagcg gttgacatct tgtataatga aatgggacaa acctttgcac cattgaaaac
     1741 atggagcaaa tttatcctta caagtgatga actttttgaa ctaaaatacg ggcaaaaagc
     1801 tgataaaaaa cgtaaacttt ataacggcac cctaaaagtt gatttgtacc aattttatgg
     1861 ggaaagggta aagcgacatc tgatatagag aggtagtaag cgtgtcagaa gaaagcaaag
     1921 aagtcgaagt aacaaaagaa tcgcaaacat tggggcttaa tgaagccaag tcaatgacaa
     1981 ttggtgaagc tgtacgcaaa cagtcagaga taaaagcagg tgttactaag gatgatagta
     2041 ttttagataa gtacattaag caacaccgtg acgaagtttc ttctcaaaag tttgatgcta
     2101 agtatacaga gcttgataca gctagtttag ataactttat caaaaaacaa agagaagcat
     2161 tgagtaaagc cggtcttgtt gatgacgaac ctgtgtctgc ggagtctgct gagcaagata
     2221 gcactttggt tgaagaagta gctgaagact tagctccaat ggagactact gcggtggtaa
     2281 ctgggattcc ggtggaagcg acagtccctg tgttagatct agatccatca gagagggtca
     2341 ttccggaacc acagataacc aaagaagagc caaaaagaga tcagtttctt tctgaagata
     2401 gccaccatcc agccaaacaa aacacgaaaa aaggttggtt aatagcttta tttctgctat
     2461 tattggccat tcttgcagtt gtatttggtt ggaaccattt cttgagacaa gacagtggta
     2521 aaacaactca aacagcaagt aaacagacaa agacctcttt gcagaccgac agtgctaaaa
     2581 aagcaacgcg cttgaaggct gctgctaaag ctttcgaaaa acaatatggt actttttata
     2641 cagatgccac aaagagcaaa ttaaaaaata gtgcctttgc tactttgcca gatttagaag
     2701 ctgccttgaa agctttggaa ggctctgctt actatgataa ggcgaaagca aaagtcgatt
     2761 cccttaagaa agctattgct gctattacag cggttaacgg taaatttgtt tccgatgttg
     2821 ttgtagatgg tgaaaaggtt tcggctgaag tcaaggctga tgctaatttt gatgatttat
     2881 caagtgcaac cttgacaaca ggaaatgcaa atcttgatgc tgtcttgcag gcaagcatca
     2941 cagaaggtcg tcagcagcta gccagcaagg ctgaggccgc taaagcagct aatgagcaag
     3001 cagcccaggc tcaagcagct caaggacaaa gtacatcagt agcaccatct ggctatggct
     3061 tgacaagtta tgaccctgca agcttacaac ggcatttatc acgtgtacct tacaatcaag
     3121 atgttattgc tgatagagct aacccatctt gggcatttaa tccaggagtt cttgaaaaaa
     3181 ttgtagcaac ctcacaagct agaggttata tttcaggcaa ccaatatatt ttagagccgg
     3241 ttaatattat taatggcaat ggttactata atatgtttaa gcctgatggc acttatttgt
     3301 tctctattaa ttgtaagacg ggttattttg taggaaatgg caaaggttat gctgatgctt
     3361 tagattactg agaaaatcaa aaaaactgag ataaggcaac ttgtctcagt tttttgatta
     3421 ataaggtatg ttgtcttaaa atctgcagat agacttgttt tttgtaacaa gtaaaatagg
     3481 tcaataggat tgttatgttt gacaacacac tagtatcaga tgacatggcg actaaaagga
     3541 tcgtctgaaa tcccttgatc aataatcaat tgagcccatt ctttggcggc aaagaggcta
     3601 tgatccttat agttcccaca ggattcaaga gttgttccag gaacatcttc ccaagtagtc
     3661 ccagttgcaa tttcttctag gctggattta atcaccttgg caatatcagt agaactgtgt
     3721 tttccccaca tgataaggtg aaaacctgtt cgacagccaa aaggagagca atcaatcatc
     3781 ccatcaatgc gttggcggat gagcttggca agtaagtgtt caatggtatg caaaccggct
     3841 gtttcaatag aattttggtt aggctgcact aggcgaacat caaaatttgt aatacgatcg
     3901 cccttgggtc caaattcttc agaaataaga cgaacataag gggcctttac aatagtatga
     3961 tctagctcaa aactttcgac gataacttct tttgtcattt caatctcctt tgttttacac
     4021 tttatcataa cacaaatagt gagaaagtgg ctacttgtgg aggagaaaag gctaagaaaa
     4081 cagatcaaag gctatcacaa tgacctgtgc gataaaagta cttacagaaa cagtaataaa
     4141 agtttcttta aaagcatttt tgtggttaag tcctgtcaat gctaagaagg tgagaaagac
     4201 accactttgg ggcacaatgg taaagatatt cgaagcaata gtggcaacac ggtgaatcat
     4261 ttcgggatta aggccttgat ctaaataata ctgggcaaaa ttaggcatga caattcctag
     4321 agcacctgaa gaagaaccgg taatagctga catagaggaa gtcaacacgg ctaaactaat
     4381 aagaggatta ccgggaatat tcaaaatgag atctgagaag aatgtgaaac ctggggcaat
     4441 catcacaacg gctccgaagg caactgcaga tgctgtcgca aaaataggtg caatagagct
     4501 gctagctcct aaattaaaga cagcaatctt attaggaata aactgtctaa agagccagct
     4561 tgcggtgaga atggcagcta aaagtgctat gaaaataatg ttcttcttaa aaaaatcgtt
     4621 tccgaaaagg ctaccggtta gggcgataat aatgagcaac aataaaggta agatactggc
     4681 aaggaaatgt gggagatttt ttgttttcac ttgtatctca ttgtcaaaag cataagtggc
     4741 ataagtttca cccattgcca tactttttgc tagacaatat ttcatataaa agagcccaaa
     4801 agtcacgcag ccgattgagc caacgatact cggaatggcg gcagcagtta gtgaggtgtc
     4861 caagtattga ataggaataa cattttgaat ggcaggtgtt ccgggtaaaa tcgtcatggt
     4921 aaaagtcgca ataccgagcc aaagaggaac ttgaattaag ttccaagcca agtccatttt
     4981 tttgaataga cttcgtgcaa gcggcaagac agcaaacatg acaacaaaga ggctgattcc
     5041 cccgtaagtt aatatagcac tgattagaaa aatagcaacg agtactttat aaggactatc
     5101 atgaccaact ttttttaaaa tataatctgc aatagaagtg gttgcaccgc ttgtttccat
     5161 taatttggct aagatagaac ctaataaaaa gatagcaaaa taattgagaa tataagtgga
     5221 gagtgccccc ataaattggt tgggttcttt tccaagtaag gttttggttg gatccatttg
     5281 attaaacaaa atgaccaagc tggtagcaag aggcgcagcg atgataatgt taacttcttt
     5341 gacagacagg taaataataa caataacacc taccaagacc cccagagaac caataatttc
     5401 tatagtttta tgacctcctt attagaaaat aaagagacag atcacaagat acagcctctt
     5461 tatctctcca aatttattga gcaatgtagc caccgtctaa gatacaggct tgacctgtaa
     5521 caccttttgc cttatcactg gcaaggaaag acacatagtc tgcaatttct tgaacgtcaa
     5581 tgaggcgttt ttgaggaaca agtgggtata gcacttcttc aagaacattt tcaaggggaa
     5641 tacctcttgt tttggaaagg tcttcaaact ggccacgaac cagtggtgtg tcaacatatc
     5701 caggacaaat ggcattgacc gtaatgcctg aatcagcagc ttctaaggca gttactttgg
     5761 tcagaccgat caagccgtgc ttagctgaat tgtaggcgga tttgccagca aaaccaatga
     5821 caccattgat agaagccata ttaataatac gaccgtgttt ttgagctttc attgtaggaa
     5881 aagcacgttt aatggcaata aatggtgctg tcaacatgat tttaatcatg aattcaaatt
     5941 tttcagttgg aaaatcttca atcatggcaa catgttgaag gcctgcgttg ttaaccaaaa
     6001 tatcaatacg accatatttt tcaacaaccg tatcaatagc agcattgatg gcttcttctt
     6061 tggtgacatc gcacacaaca ctaaaagcgt catagccttc tttagtatag tcagcaacaa
     6121 cctgatccag tttttcttga ttaatgtcgg aaaaaacaac cgttttacct tcttttaata
     6181 aggtttcccc gatttgtttt ccaattccgc ttgcagcacc agtgataaat gcaacttctt
     6241 tagtatgtgt catgatacta tcctcgtctt tcttttggtt agtctttagt ttgttttatc
     6301 aattagggta acttcggtaa cgttttgcac gtcttcaaag gtgtaatctg ggttaatttc
     6361 aagggcgcat agaccttcat cttgatattc aaagactccc atttcagtaa tgattaggtt
     6421 aacaacattt tgagcagtca gaggaagggt gcatttgtct aaaatttttg ctttgccttt
     6481 attggtatgc tccattgcaa caatgacttt tttagcacct accaataagt ccatggcccc
     6541 tcccatccca ggaaccattt ttccaggaat caggtaatta gcaatgctag cttctttatc
     6601 aacttgtaag gcacctaaga cggttgcggc cacatggccg cctcgaataa taccaaagga
     6661 agttgagcta tcgaagaaag cccctccagg agcaatacta acaggttgtc caccagcatt
     6721 aacaatacta ggatcgtagt gatcatctgt taatccagtg agaccgacaa aaccattttc
     6781 ggactgcagg gtaatggtta caccttcagg aatatagttg gctacttttg ttggcaaacc
     6841 tatgcctaag ttgaccagtg tattgtgttc taattcttgt gcaacgcgct tagcaatacg
     6901 tgtttgaatc tcttcttttg aaagaacagt agctgatgtt cccatgttag gctcctttca
     6961 ctaagtagtt aacaaaaatg ccaggagtat ggacaaaatt aggatccatt tgccccacgt
     7021 caacaatttc tcttgcttca acaatggtgg ttttcgcatt agcagccatc acatgattaa
     7081 agttattttc agaacctgcg tattgcaaat tgccattttt atctgccttg ttagcaaaaa
     7141 taagtgcgat atcagctttc aaaggttttt caagaaggta gtctttgccg tcaatagtaa
     7201 tgacttcttt tcctttggca acttcggttc ctattccagt aggtgttaag aaaccgccta
     7261 gcccaaaacc gccactgcgg atgcgctcag ctaatgttcc ctgaggtaca agatcaatta
     7321 cagtctcgcc ctcagtcatt tgtcggcccg cttcacggtt gagaccgata tgtgatgcaa
     7381 tatatagttg aaaactgttt attggcaacc atcttaccaa cgcctttgtc aggaaaaaca
     7441 gcatcgttac agattaaagt gaggtctttg acaccttttt ccacaagtgc atcaattaat
     7501 ttttctggtg tgccatttgt catgaagcca ccaaccataa tggtatcacc atctttaacg
     7561 tgagctaccg cttcttttat tatgatttct ttacctattt tttctaccaa ctttcacgag
     7621 actaagtatt gtttttcacg ataacagcag ttccttgtcc tccaccaatg caaagtgttg
     7681 ctagtcctct ggttacttgg cgttttttca tttcgtggat taatgtgact agaatgcgag
     7741 cgccactggc accaatagga tgacctaaag cgatggctcc tccattaaca ttcacgattt
     7801 ctgggtttaa ctttaaatct tttaaaaccg ccaaggactg tgctgcaaat gcttcattgg
     7861 actccactaa gtccaaatca gaaattgtta atccagcttt cttaagggct ttttgagtgg
     7921 caggaattgg tccagttccc atcagttcag gtgcaacacc tgcactagca tagctttcaa
     7981 tagtgactaa aggtgttaac cctaattcaa gagctttttc ttcagtcatt aacatcaata
     8041 aggctgcacc atcgtttatt cctgaggcat tcccagcagt aacggttcct tctttaggga
     8101 ggaaagcagg acgtaattgc tgtaattttt caaggcttgt gtcaactttt gggtattcat
     8161 ccgtagtaac aattaaagga tctttgcgtc gttgaggcac agagacaggg acaatttcgt
     8221 cagcaaaacg atgatttcct aatgccttag cagctttttc ttggctttct aaagcaaatt
     8281 gatcttgctc ttctcttgag atgccatatt tctgagcaac gttttcagca gtaataccca
     8341 tgtggtaatt attaaaagca tctgttaagc catcatgaac aagagtatca atcaattgac
     8401 tattaccaag acgttgacca aagcggtgct gagttgagac ataagctgct tgtgacatgt
     8461 tttccacacc accagcaata acgatatcag cgtcaccaag catgatgctt tgcgcagcta
     8521 attggattgc tttaagccca gaaccgcaaa ccatatctaa tgtgaaagct gttttatctt
     8581 caggaattcc tgagtggact gcaacttgtc ttgccacatt ttgtcctagg cctgcatgaa
     8641 ggacgtttcc taggatgact tcatcaacca tttcaggctt tacttgcgtt ttatcaagga
     8701 tttttttcag tacagttgtt ccaagttcaa cagcagagac acttttaaag gagcctccaa
     8761 aagctccgat aggagttcgt aaagcttcaa cgataactat gtttttcata aaataaacat
     8821 cctttcagtt ttagaaagcg ttttctatat ctctaagata acaagaaacc gctcaaaata
     8881 aaaatataga atttttataa gcatataaat ttttttgata gatttggcaa tagcaattaa
     8941 aaatcactta tgtcataatg gaattaatac tatcggaaaa aagactaata gacaagggga
     9001 taaccatttg gatattaaac aaattcgtta ttttatcgca atcgttgaaa atcattttaa
     9061 cttgaaccag gcggcggaac tgttgtatgt gtcgcagccg acacttagca tgatgatcaa
     9121 tgattttgaa aaaagggaaa atgttaagct cttcaaacga aaaagaggtc gtattattgg
     9181 gttaacctat cttggtgata attattataa ggacgctcaa aaggttctca gtctctatga
     9241 tgacatgttt ttgaaattgc atgatcacag taaagggtta aagggaagta tcaatattgg
     9301 tattcctcct cttattttat cagttgtttt ctcagaagta atgccaaaac tgattttaga
     9361 aaatccggga attcaattca atcttaagga gattggtgct taccaactaa aaaatgaact
     9421 actcgtggga aatgttgacg tcgcagtctt gttatcccca acaggcatcg ctgataattt
     9481 ggttgagact tatgaaatcc agagatcaga attatctgta tgcctgtctc cacgccatcg
     9541 tttagcttct aaaaaagtta ttcagtggga agatttaaca gatgaacaac ttgccctatt
     9601 tgatcctagt ttcatggttc accatttagt gcttgaagct tgcgagcgtc accaagtcag
     9661 acctaatatt attttgacgt cctcttcctg ggattttatg cttaattcta ccaagattaa
     9721 tcacaacgtc ttaactattt gtcccaaacc tattacagaa ttataccaat tcaaggacat
     9781 taagtgtatt ccaatggaac gtcccatttc ttggcgtgtt gtcttaacac gcctccggaa
     9841 aaaaagttat tctgaaatag aagcctacat catggacgac ttgctacagt ctttttttaa
     9901 gtagggttct caactacttg gcccgaatgt taattttttt aaaaatgaat tggaattgtg
     9961 taaacgctca atttatgata aactaagaga tggctttcta ttaatgaaat ctagaatata
    10021 ttgaggtaca aacatggtta atattatttt attaattgtt tctgccctca ttggtttaat
    10081 attaggttat gcacttattt cgattagact caagtctgcg aaggaagctg cagagttgac
    10141 tcttttaaac gctgaacaag aagctgttga tattcgtggc aaagcagaag tagatgctga
    10201 acacatcaaa aaaacagcta aacgtgaaag taaagcaaat cgtaaagaat tacttttaga
    10261 agcaaaagaa gaggcaagaa aatatcgtga agagattgaa caagaattta agtctgaaag
    10321 acaagagctt aaacaactcg agacacgctt agcggagcgc tccttaactc ttgaccgtaa
    10381 agatgaaaac ctatcaagta aagaaaaggt actagatagt aaagaacaaa gtctgaccga
    10441 taaatctaaa cacattgatg agcggcaact tcaagtggaa aaacttgaag aggagaaaaa
    10501 agcagaactg gaaaaagttg ctgcgatgac gattgcagaa gcgcgtgaag tgattttaat
    10561 ggagacggaa aacaaactga cccatgaaat tgcgacgcgc attcgagatg ccgaacgtga
    10621 catcaaggac cgaacagtta aaacagccaa ggacttgtta gcgcaagcca tgcaacgcct
    10681 tgctggtgag tatgtgactg aacaaactat taccagtgtc catctcccag acgacaacat
    10741 gaagggccga attattggac gtgaaggccg taatattcgt actttagaga gcttgactgg
    10801 cattgacgtt attattgacg atactcctga agttgttatc ttatcaggat ttgatcctat
    10861 tcgacgtgaa attgctcgta tgaccttgga atctctgatt gctgatggtc gcatccatcc
    10921 agctcgtatc gaggaattgg ttgagaaaaa tcgtcttgaa atggataatc gtattcgtga
    10981 gtacggtgaa gctgcagcct atgagattgg tgcaccaaac cttcatcctg atttgattaa
    11041 aatcatggga cgcctgcaat tccgtacctc gtttggtcaa aatgtcctac gtcactctgt
    11101 tgaggttggt aagttagctg gtattttagc tggtgagtta ggtgaaaatg ttgctcttgc
    11161 ccgccgtgct ggtttcttgc acgatatggg taaagctatt gaccgtgagg ttgaaggtag
    11221 tcacgttgag attggaatgg aatttgcacg taaatacaaa gaacatccag ttgttgtcaa
    11281 cactattgct agccaccacg gagatgtgga gccagattct gtgatcgctg tgctagtagc
    11341 tgcagcagac gctctcagtt cggctcgtcc aggcgctcgt aatgagtcaa tggagaatta
    11401 catcaagcgt cttcgtgatt tagaagaaat cgcgacaagt tttgatgggg tacaaaatag
    11461 ttttgcgcta caagctggac gtgagattcg tattatggtt caacctgaaa aaatttcaga
    11521 tgatcaggtt gtcattttgt cgcataaagt aagagaaaaa attgaaaaca atctagatta
    11581 cccaggaaat attaaagtaa ctgttattcg tgagatgaga gcggttgatt atgccaagta
    11641 gaaaaagagg aattatcctc tttttcttta tgacttgcct ttgaaaatgt tcttgaaaaa
    11701 gactagcctt tttgataaac tattaaaaag caactaataa ggagaataca atgtctgaac
    11761 gcggtttgtt aatcgtcttt tctggccctt caggtgtcgg aaaaggaacg gtaagacaag
    11821 aaattttttc aacgccagat cataaatttg aatattcggt ttcaatgacg actcgtccgc
    11881 aaagaccggg cgaggttgat ggggttgatt atttcttccg cacacgtgaa gaatttgaag
    11941 aactcattaa aactggtcaa atgctagagt atgctgaata tgttggtaat tactatggca
    12001 cccctttaac ttatgttaat gagactcttg ataagggaat tgatgtcttt ttagaaattg
    12061 aagttcaagg agctcttcag gttaagtcaa aagtaccaga tggtgttttt gtttttttaa
    12121 caccaccaga tttagatgaa ttagaagacc gcctggttgg tcgtggtaca gatagtcaag
    12181 aagtcatagc tcaacgtatt gaaagagcaa aagaagaaat tgcccttatg cgcgaatacg
    12241 attatgcagt tgttaatgat gaagttgcct tggcagctga gcgggttaag cgtatcattg
    12301 agacagaaca ctttcgtgtg gagcgcgtga ttggtcgtta tgataaaatg attaaaataa
    12361 caaaaaaaac ctttaaagct aaatagaaaa gagtaagcat tatgatgtta aaaccttcca
    12421 ttgatacctt attagataaa gtatcatcca agtattcact tgtgatttta caagccaaac
    12481 gtgcccatga actagaagct ggtgccactc caactcaaga atttaaatca gtgaagtcaa
    12541 cacttcaggc acttgaagaa attgaatcag gaaacgttgt gattcaccca gatccttcag
    12601 ctaaacgtga agctgtccgt gctaagattg aggcagaacg cttggccaaa gaggaagagg
    12661 aacgtaaaat caaagaacaa attgctaaag aaaaagaaga agaaggagaa aaaatctaag
    12721 gtttcagaaa tcttagattt tttcatattt agtacgatgt ccatgacaga aaaggagaag
    12781 caaatggaaa aagtagctca tgtgattgta gatattccat tgatgcaaac ggacaagcca
    12841 ttttcttatg gtattccaaa agagttagtt tctctcgtgc agcttggttc tcgtgtgcat
    12901 gttccttttg gaaaagggaa tcgtttgttg caaggcttta ttattggttt tggccaagaa
    12961 gattcaagct ctcttaaact gattcagacc gttttagatc ctgagccagt cctaaaccaa
    13021 gaacaattga cccttgctga tcagcttcga aaaacagttt tttcttataa aatcacgctc
    13081 ttaaaagcaa tgattcctaa tcttcttaac tctaactatg ataaagtgct aaggccagag
    13141 tcaggtctaa aaaagcctga tagagacttt ctttttgaag gaaaaccatc tgtcttatat
    13201 tctaccttag accgcgagaa ggaaaaaata gctcttaagg ccatacaagc tgggcatatt
    13261 actgttagtt atttagccaa agacaagaaa aacttaaaga cagaaaaata ttatcacgtc
    13321 gacttagacg ccttagcggt acatcccatc tctagccgtg ccaaaaagcg tcaattatta
    13381 aaagactatc tgctaaccca tacgaaggaa gctaaacttg caacgcttta tcaagccttt
    13441 tctagagatg tggtggctta ctttgtgaca aatcacttaa ttcgtattga cgagcgtcaa
    13501 attgatcgca gtgagcccta ttttgaccag atcaaaccat cttctttttt aaccttaaat
    13561 gaacagcaag cgtcagctgt tactgaaatt gttgagcaaa ttgggaaacc gagtaaacct
    13621 tttttgattg aagggattac aggttcgggc aagacagaag tttatcttca tatcatcgaa
    13681 gcggttttaa aacaggataa gacagccatt gttttagtac cagaaatttc gttgactcct
    13741 cagatgacct ctcgttttat ttcccgtttc ggaaagcaag tggctattat gcattcaggc
    13801 ttatcagatg gtgaaaagtt tgatgagtgg cgcaagataa aaacaggaca ggctaaggtt
    13861 gtggttggtg cccgctctgc tattttctcc ccactagagc ggattggtgc aattattatt
    13921 gatgaggagc atgaatcaac ctataaacaa gaaagcaacc ctagatacca tgccagagag
    13981 gttgccctgc tcagagctaa acaccatcaa gcagttgtgg ttatgggctc tgctacgcca
    14041 agtattgaaa gccgagcaag ggctagtaag ggtgtctatc attttattca attaacgcaa
    14101 agagcaaacc ctcttgctaa gattccagaa gtaacgattg ttgattttcg tgattatatt
    14161 ggacagcaag cagttagtaa ttttacccca tatttgattg ataagattaa agaacgtttg
    14221 gttaaaaaag aacaagttgt cttaatgtta aatagacgtg gctattctag ttttgtgatg
    14281 tgtcgggact gtggttatgt ggacaaatgt cccaactgtg atatctcttt aacccttcac
    14341 atggatacta aaactatgaa ttgccattac tgtgggtttc aaaaacctat cccaattact
    14401 tgtcctgagt gtcatagcaa tagtatccgc tattatggca ctggaactca aaaagctttt
    14461 gatgaattgc aaggggttat tccagaagct aagatcttga gaatggatgt tgacaccaca
    14521 cgaaaaaaaa gaagtcacaa aaccatacta gatagttttg gtcgccagga agcagatatt
    14581 ttgttaggga cgcaaatgat tgccaaagga ctggattttc caaatgtgac tttagttggg
    14641 gtactaaatg cagatacctc cttaaatttg ccggattttc gagcgtcaga aaaaacgttt
    14701 caattgttaa cacaggttgc tggtcgggca ggccgtgccc ataagccagg agaagtcttg
    14761 attcaaactt ataatcccga tcactatgct attcaattag ctaagaaaca agattttgaa
    14821 gccttttacc gttatgaaat ggcgattcgc cgtcagatgg cttatccccc ttactacttt
    14881 acagtgggta ttacgctttc gcatcgttta gaagcatcgg ttgtaaaaaa agcttatcaa
    14941 gttacagaac tgctaaagag tcacttatcg gataatatta aaatattagg accgactcct
    15001 aagccaattg ctagaacaca caatttatac cactatcaaa ttttgttaaa ataccgtttt
    15061 gaagataacc tagaagagac gctcaatcgt attcttgatt ggagtcaaga agcagataat
    15121 cgtcatttaa aactaattat tgattgtgaa ccgcaacaat ttttataagg taaatcacta
    15181 aatctaggat gatcagttat gatataataa aaatacaaag aggaaagtca tgataaaatt
    15241 attatttatg ggaactcccc aattttcagc aactgtattg aagggcttgc tagataatcc
    15301 tgcctatgag attttaggag tggttactca gcctgatcga gctgttggac gcaaaaaaga
    15361 tattaaggta acacctgtaa agcaattagc tcttgagcat ggaatatcaa tctatcagcc
    15421 ggaaaaatta tcaggctctc aagaactcat tgagattatg ggacttggtg cagatggtat
    15481 tattacagca gcatttggcc aatttttacc aacaatatta cttgattcag tttcatttgc
    15541 tattaatgta catgcatcat tgttacctaa atatcgaggt ggagcaccta tccattatgc
    15601 tattatgaat ggagataaag aggccggcgt tactattatg gaaatgatta aagaaatgga
    15661 tgcaggtgac atggtcgcta aagcaagcac tccaattctt gaaaccgata atgtaggtac
    15721 tctttttgag aaattagcca tcattggtcg agacttactt ttggatagtt tgccagctta
    15781 tctttctgga gaattaaaac ctattcctca agatcacagc caagctactt tttcacctaa
    15841 tatctctcca gaacaagaaa aattagattg gacaatgtct gatcaagaag tgttcaatca
    15901 tattcgaggt atgaaccctt ggccggtagc gcatacgttt ttagaggggc aacgtttaaa
    15961 aatttacgaa gctcaactag ctgaaggaga gggattacct ggacaagtta ttgtcaaaac
    16021 caaaaaatct ttggtaatag ctactggaca aggtgctttg tccttgatcg ttgtgcaacc
    16081 ggctggtaaa cctaaaatgt caataataga ctttttaaac ggcattggta gaaaacttga
    16141 agtaggggac atcattggca gataattgga aaaaaagtac gcgaggcaaa gccctccttg
    16201 tcattgaagc tatttttgat cagggggctt acacaaacat agcgcttaat cagcaactaa
    16261 gcaataaagc tttatctgct aaggacagag ctctattaac tgagattgta tatggtaccg
    16321 tttcacgtaa aattagcttg gaatggtatt tagcccatta tgtcaaagac cgagataaat
    16381 tggacaaatg ggtttattat ctccttatgc tcagtcttta tcaactgact tatttagata
    16441 agttaccagc gcatgccatt gttaacgatg cggttggtat tgccaaaaat cgtggcaata
    16501 aaaaaggtgc agagaagttt gtcaatgcta ttttacgtca attcaccagc catcctctcc
    16561 cagatatgga aacaattaag cgtcgaaata agtattattc ggtcaagtat tcactgcctg
    16621 tttggttggt taaaaagtta gaagaccaat ttggctccga cagagcagtg gctattatgg
    16681 agagtttatt tgtgcgtagt aaggctagta ttcgcgtgac ggaccctttg aagttagaag
    16741 aagttgctga ggctttagat gcagaacgtt cccttttatc agcaactggt ttaaccaaag
    16801 cgtcaggtca ttttgcagcc agtgattatt tcacaaatgg ggacatcact attcaagatg
    16861 aaagcagtca gcttgtagct cctactttaa acattgacgg tgacgacata attcttgatg
    16921 cgtgtagcgc tccagggggc aagacctctc atatagcatc ttacttaaaa acaggtaaag
    16981 ttattgcctt agatctctat gatcataaac tagagttggt gaaggaaaat gctaaccgct
    17041 taggagttgc tgataacatt gagacaagaa aactagatgc cagagaggtt catcgtcatt
    17101 ttgaaaagga tagctttgat aagattttgg tggatgctcc ttgctcagga attggtttga
    17161 ttcgtcgaaa acccgatatc aaatataata aagaaagtca agattttaat gctctacagg
    17221 ctatccagct agagatattg tctagtgttt gtcaaacctt gcgtaaaggt ggtataataa
    17281 catacagtac ttgcactatt tttgatgagg agaatcgtca agtcatcgaa gcatttttac
    17341 agagtcatcc taattttgaa caagtaaaac tgaaccatac acaagcagat attgtcaagg
    17401 atggttatct tattatcaca ccagaacaat accagactga tggcttcttt attggacaag
    17461 tcagacgagt cttgtagtat tttaggagga aactgactga gtcagaaaaa acgtatgaag
    17521 atttcattaa aaacagacat tgggcaaaaa cgctcaaaca atcaagattt tatcaataag
    17581 tttgataata aaaaaggcat cactttagtg attttagcag acggtatggg tggccacaga
    17641 gctggaaata ttgccagtga gatgacagtc acagacctgg gaagagaatg ggtaaaaaca
    17701 gacttcacag agttaagtca gattagagat tggttatttg aaactatcca gtcagaaaat
    17761 caacggattt atgatctagg ccaatcagaa gactttaaag gaatgggaac gactgttgaa
    17821 gcagtagctt tagttgaatc tagcgccatt tatgctcata ttggtgattc acggattggt
    17881 cttgtccatg atgggcatta taccttattg accagtgatc attctttagt gaatgagttg
    17941 gttaaggcag gacagatcac agaagaagaa gctgctagcc atccacaaag aaatattatc
    18001 actcaatcta ttggtcaagc ttccccagta gagcctgatt taggagtgag ggttttggag
    18061 ccaggggatt atcttgtcat taacagtgat ggtctaacca atatgatttc aaatgatgaa
    18121 attgtgacta tcttgggcag taaggttagt cttgatgaaa aaaaccaaga aatgattgac
    18181 ttagctaacc taagaggagg cctagataat attacaatcg ctttggttca caacgaaagt
    18241 gaggacgtcg aatgattcag attggcaaat tatttgctgg tcgttatcgc attctaaaat
    18301 ctattggccg cggtggtatg gcggatgttt atttagcaaa tgacttgatc ttggataatg
    18361 aagacgttgc aatcaaggtc ttgcgtacca attatcaaac agatcaggta gcagttgcgc
    18421 gtttccaacg agaagcgcga gccatggctg aattgaacca tcccaatatt gttgccatcc
    18481 gggatatagg tgaagaagac ggacagcaat ttttagtaat ggaatatgtg gatggtgctg
    18541 acctaaagag atacattcaa aatcatgctc cattatctaa taatgaagtg gttagaatta
    18601 tggaagaagt cctttctgct atgactttag cccaccaaaa aggaattgta cacagagatt
    18661 taaaacctca aaatatccta ctaactaagg agggtgttgt caaagtaact gatttcggca
    18721 tcgcagtagc ctttgcagaa acaagcttga cacaaactaa ttcgatgtta ggcagtgttc
    18781 attacttgtc tccagaacag gctcgcggct ccaaagcgac gattcaaagt gatatttatg
    18841 cgatggggat tatgctcttt gagatgttga caggccatat cccttatgat ggcgatagtg
    18901 ctgttacgat tgccttgcaa cattttcaaa aacctcttcc atctattatc gaggagaacc
    18961 acaatgtgcc acaagctttg gagaatgttg ttattcgagc aacagccaag aaattaagtg
    19021 atcgttacgg gtcaaccttt gaaatgagtc gtgacttaat gacggcgctt agttataatc
    19081 gtagtcggga gcataagatt atctttgagg atgttgaaag taccaaaccc ctccccaaag
    19141 tggcctcagg tcccaccgct tctgtaaaat tgtctccccc taccccaaca gtgttaacac
    19201 aggaaagtcg attagatcaa actaatcaaa cagatgcttt acagcccccc accaaaaaga
    19261 aaaaaagtgg tcgtttttta ggtactttat tcaaaattct tttttctttc tttattgtag
    19321 gtgtagcact ctttacttat cttatactaa ctaaaccaac ttctgtgaaa gtgcctaatg
    19381 tagcaggcac tagtcttaaa gttgccaaac aagaactgta tgatgttggg ctaaaagtgg
    19441 gtaaaatcag gcaaattgag agtgatacgg ttgctgaggg aaatgtagtt agaacagatc
    19501 ctaaagcagg aacagctaag aggcaaggct caagcattac gctttatgtg tcaattggaa
    19561 acaaaggttt tgacatggaa aactacaaag gactagatta tcaagaagct atgaatagtt
    19621 tgatagaaac ttatggtgtt ccaaaatcaa aaatcaaaat tgagcgcatt gtaactaatg
    19681 aatatcctga aaatacagtc atcagtcaat cgccaagtgc gggtgataaa tttaatccaa
    19741 acggaaagtc taaaattacg ctcagtgttg ctgttagtga tacgatcact atgcctatgg
    19801 taacagaata tagttatgca gatgcagtca ataccttaac agctttaggt atagatgcat
    19861 ctagaataaa agcttatgtg ccaagctcta gctcagcaac gggctttgtg ccaattcatt
    19921 ctcctagttc taaagctatt gtcagtggtc aatctcctta ctatggaacg tctttgagtc
    19981 tgtctgataa aggagagctt agtctttacc tttatccaga agaaacacac tcttctagta
    20041 gctcatcgag ttcaacgtca agttcaaaca gttcttcaac aaatgatagt actgcaccag
    20101 gtagcaacac tgaattaagc ccatcagaaa ctactgctca aacaccttaa caaagaatag
    20161 ttagtctcat gttttactgg catgccacta actattcttt ttaactactc gtcatataaa
    20221 gatgcaattt tccaggaatt ctgataaaat acaaagaaat aaggggaaac taatgaaaaa
    20281 atttcaattc tttttactta tcgagtgtat actgctagct atgggtatca tgacaatctt
    20341 ggataatgat ttatcaagtt ttatcctaat tctcgttctt atcttattag cattacgctt
    20401 ttataaccaa gatagccgca ataatttttt gctaactgtc agtttattgt ttcttttttt
    20461 aattttcatg cttaatccct acattatcat ggctgttctt ttggggatag tttacatttt
    20521 cattaaccat ttttcacaag tcaaaaagaa gaatcgcttt gccttgattc gtttcaagga
    20581 agaaaagatt gaagtgaata ataccaagca tcaatggatt ggtactgcta attatgaaag
    20641 tgattattat tgttttgatg acattaacat cattcgaatc tcaggaaatg acacggttga
    20701 cttaaccaat gttattgtga caggaatgga taatatcatt gtgatacgta aaatttttgg
    20761 aaatacgact atattggtac ctattgatgt cactgttact ttagatgtta gttccatata
    20821 cggaagcgtt gattttttta gatgtcagca atatgatttg cgcaacgaat ctattaagtt
    20881 aaaagaaacg gataaccaat cccttaaaaa agtcaaaatt atcgttacaa ctattgccgg
    20941 aaatgttgag gtggataggc gatgaaaaaa cgttactatg ctcttgtttg gctctactca
    21001 accattacca ttttatctat tgtttttgtg gtcatggata atttaggaat cacttttaac
    21061 taccttcgca accatttatg gcaggttgaa cgtctagggt tttcgatttt attgttgatt
    21121 gtttctgtga ctttattatt gttgttattg tggattatta tggatgataa cagtaagcgt
    21181 aacatcaacc aaaatctaaa atatattctc aataatcgac gactctatct agatgagaca
    21241 tcagaaatca atactaattt aagtcgactt tctaaaaaaa tgtctcacct gactgctaac
    21301 atgcaaaaga aagaaagtgc ttatattctt gatagccaag aagttgtaaa acaagagcgc
    21361 aaacgaattg caagagattt acatgacaca gtcagtcaag agctattcgc ttcgtcattg
    21421 attttatcag gaatttcgat gagtttggaa caactggaca aaacacaatt acaaacacag
    21481 ttaacaacgg ttgaagcaat gttgcaaaat gctcaaaatg atctacgcat tctccttttg
    21541 catcttagac ctaccgagct agctaatcgg actttatctg agggccttca tatgattctt
    21601 aaggaattaa cagataaaag tgatattgaa gtcatttata aggaaaccat tgctcagctt
    21661 cccaaaacaa tggaagataa tctttttaga attgcccaag aattcattag caacacgtta
    21721 aaacatgcta aagctagtcg aattgaagtt tatctcaatc aaacctcaac agaattacaa
    21781 ttgaagatga ttgatgatgg tgtaggattt gatatggatc aggtaaggga tttgagttat
    21841 ggtctgaaga atattgaaga ccgtgtcaat gatttagcag gaaacctaca tttaattagt
    21901 caaaaaggca aaggagtttc catggatatt agactgccga tagtgaaggg agatgacgat
    21961 gagtaagata aaagtgatat tggtcgatga tcatgaaatg gtccgcatgg gactcaagag
    22021 ttttttgaat ttacaagctg atattgatgt cgttggtgag gcctctaatg gacgtgaagg
    22081 ggttgatttg gcattggctt tgaagccaga tgttttggtt atggatctag tgatgccaga
    22141 gttaggcggt gttgaggcaa ctttagaagt cctaaaaaaa tggaaagagg ctaaggtact
    22201 tgtgttaact tcctatctag ataatgaaaa gatataccct gtcattgatg caggagcgaa
    22261 gggttatatg ttaaaaacat cgagtgcggc tgaaatttta aatgccattc gcaaggtttc
    22321 aaagggagag ttagctattg aaacagaagt tgacaaaaaa attaaggcgc atgatcaaca
    22381 ccctgacttg catgaggaac taacagcgcg tgagtatgat attttacacc ttttagctaa
    22441 agggtatgat aatcagacca tcgctgatga actctttatt tccttaaaaa ccgtcaaaac
    22501 acatgtgtcc aatattctag ccaagttaga agttgatgac cgaacccaag cggttgtcta
    22561 tgcttttcga caccatttag tcccccaaga cgataattaa tgctataata tagggtatag
    22621 caaattggag gaaagtatgg acgcaaaact aaaatacaaa gctaaaaaaa tcaaaatggt
    22681 gttctttgat attgatgata ccttgagggt caaagatacg ggttatatgc ctgagtcgat
    22741 tcaaagagta tttaaggctt tgaaggcaaa aggcatcttg gttggtattg cttcaggtcg
    22801 cgctcgttac ggtgttccac aagaagttca agatttacat gctgactatt gtgtcaaatt
    22861 aaatggagct tatgtcaaag acgatgctaa aactattatt ttccaagctc cgattccagc
    22921 agatgttgtt gtggcctata aaaaatgggc ggatgatatg ggcatttttt atggaatggc
    22981 gggtcgtcat gaagcggtgt tgtccgctcg aaatgacatg attagcaatg ctattgataa
    23041 tgtctacgct caactagagg tctgtccgga ttataatgag tatcatgatg tttaccagat
    23101 gtggactttc gaggacaagg gagatggctt gcagttgcct gctgaattag cagagcacct
    23161 tcgtttggtc agatggcacg ataactcatc agatgttgtc ttgaaaggta cgtcaaaagc
    23221 gctaggtgtt tcaaaagtag ttgaccattt aggattaaaa cctgaaaaca ttttggtgtt
    23281 tggtgatgaa ctaaacgact tagagttatt tgactatgct ggtatcagta ttgcaatggg
    23341 agtatcacac ccacgtttgc aagaaaaagc agatgttatc acaaaaaaag ttgaagaaga
    23401 tggcatttta tatgccttgg aggaattagg attgattgac aaagaattac agtttccaca
    23461 attggatttg gcaaaccata aaggaccaaa agcaaccatt aaaacaaacc atggcgatat
    23521 gaccctggtc ttgttcccag agcatgcccc taagacggtt gctaacttct taggattagc
    23581 taaagaaggg tactatgatg ggattatctt ccaccgtatt attcctgaat ttatgattca
    23641 agggggcgat ccaacaggaa caggaatggg tggacaatcc atctatggtg aaagttttga
    23701 agatgaattt tcggatgaac tttataatct ccgtggggcc ttgtcgatgg ctaatgcggg
    23761 accaaatact aacggcagtc agtactttat tgttcaaaat agtaaggttc cttatgccaa
    23821 aaaagaacta gaacgtggtg gttggccggc tccaattgca gcttcttacg ctgaaaatgg
    23881 tggaactcct caccttgatc gtcgtcatac ggtctttggt cagcttgtga acgaaacttc
    23941 attccaagtt ttggatttga ttgctggcgt tgaaactggc gcacaggata aacctaaaga
    24001 agacgttatc attgaaacaa tcgaggtatt tgactaatga aaattggcga caaactgcat
    24061 gggaccatta caggaattaa accatacggt gcctttgttg cccttgacaa tgggacaaca
    24121 ggtctcattc atatttcaga aataaaaacg ggatttattg atgatattga tcaattattg
    24181 gctataggca accaggtttt agtacaagta attgatattg atgaatatag taaaaaggca
    24241 agtttatcta tgcggacctt agcagaagaa aaacagcatt ttttccatcg tcatcgttat
    24301 tccaacagtc ggcacaagat tggctttagg ccacttgaag agcagttacc tcagtggatt
    24361 gaagaaagcc tacagttttt aaaaaagaac actaaaaaga gtctggaacc ttagtagttc
    24421 caaacccttt tttatttcgt ctcaattact ggtacatcat agagttcagt cgataaatag
    24481 cgttcgccat tatctggtaa aatagttaat acatgtttgc cttttcctaa ctgtttagcg
    24541 acttcaatag cggcgtaaag agcagctcca gaagaaatcc caaccaggaa gccttcctta
    24601 gctcctgttc gtcgagcagt ttctaaagca tctttcgatt taacacggat aatttggtca
    24661 taggctttgg tatctaacgt gtttgggata aatccagctg atataccttg gattttatgt
    24721 ggtccaggct cttgaccaga taagacagca gattcttcag cttcaacagc atagatagca
    24781 gtttcagggc tagctttttt caagacatgt gaaacaccag aaagtgttcc tccagtacca
    24841 acaccagata cgaatgcatc taaagaaatc tccttaaaag cttccaaaat ttcttgagct
    24901 gttgtttttt catggatgct tggattggca gggttattaa attgcatagg catccaagca
    24961 cctagttcta ttgctaaagt ttcagctttt gcaatagccc ctttcatacc ttctgctcca
    25021 ggtgttaaga caagctctgc accataagcc tgaatgattt gtcgtctttc caagctcatc
    25081 gtttcgggca taacaataat gactcgatac cctttagcag cacctaccca tgcaagacca
    25141 atacctgtat taccgcttgt tggttcgata ataacgtcac caggacttat cagaccttca
    25201 gcttcagcag cttcaatcat cgataaagca atacgatctt taacagaaga tcctgggtta
    25261 aaagcttcta attttacata aacgtcagca gcttcgtttg gaattaaacg gttaagtttg
    25321 ataataggtg tttgacctac taattctgtt atagttttgt aaattttagt catagtttag
    25381 cctcttttct tgatacaaaa taaatacgct gtgtatttaa gtataaggag agatattatt
    25441 aaagtaaaat acagatttac tatcactctg ataataaagc catatcactt ttgttgatga
    25501 ttaatcacag gggaattcaa taactttgga tccgattttc tcacagctta tctgaccatg
    25561 ataataattt gttaaagtat caatgagatt ttctacacgt tcagtatcac aataaagtgt
    25621 tgtctttatc gtgtctgaaa acttagtttc tgtttcggtt aaagccaatt gatctagcaa
    25681 gttagaatat agttgatatt ggggataaga aagtgtgatt tctagtccta tctgctgttt
    25741 aacttcaatg atgccaaatc gtttaatagc tgttgcggtc atgctagagt atgctctgat
    25801 taacccacct gttcctaatt taataccacc aaagtaacgc gttaccacaa ctactacatt
    25861 tgttaaacac tgtttttcta acacagaaag gataggtatt ccagcagttc ctgaaggttc
    25921 accatcatca cttgagcgct tgatctgact gttgttaccg ataatcatcg caaagcagga
    25981 atgattagct ttgtaatgtt cttttttgat agcattaaca aaattcttac catcttcttc
    26041 agtgctcact cgtttgatat gacaaatgaa acgagatttt ttaatacttt cttcgaaaaa
    26101 accactggct ttgattgtct taaaatgttc cataaaaaaa ttgtacaaga aaagataaaa
    26161 attagcaaaa aaagcgaata ataaagtatg gagtctattg aaaatagtta tgggcggttg
    26221 ttgctagaaa gccagttacc agattcagca aggcaacttg ctcaaccttt aaaaagtgta
    26281 gtaatatcga gaggaaaaat gatttgtcaa cgttgtcact atcagcttga tgaagaagca
    26341 cgattgccaa gtggagccta ttattgccga ttttgtcttg tctttggacg aaatcaatca
    26401 gataaattga tctatgctat cccacctatg cattttccaa aaggtaatta tctggtttgg
    26461 agcggtcaac taactgctta tcaagagatg atttcccaac agttattaat caatatgcaa
    26521 aatcaaaaaa caacattagt tcatgctgta actggtgcag gaaaaacaga aatgatttat
    26581 gctgcgattg aggcggtaat taacacaggt ggctgggttt gcattgccag cccaagagta
    26641 gatgtttgtg tagaggtggc tactcgttta tcccaagcct tctcctgcag catctgttta
    26701 atgcacgcag agtctcttcc ttatcaaaga gctcctatta tagtagccac aacgcatcag
    26761 ctgctaaaat ttcacaaggc ttttgatctc ttaattattg atgaagtaga cgcattccct
    26821 tttgtaaata atattcaact tcattatgca gcaagtcagg ctttaaaaga aggtggcgca
    26881 aagattttat taactgcaac ttcaactaga actttagaaa gaaaggtaaa caagggagaa
    26941 gtagtcaagc tgacattggc gagacgcttt cataataggc cattagttat tccgaaattt
    27001 ataagaagtt ttaatctatt taaaatgata catcggcaaa aacttcctcc taaaatttta
    27061 aaatacttaa aaaaacaaag aaaaacaggc tatccccttt taatttttct acccactatt
    27121 ataatggcag aatctgttac agcaatctta aaagagttat tgcccgccga acaaatagct
    27181 tgtgtttcta gtcaatcaca aaacaggaaa gaaggtatca cagcttttcg tcaagggaaa
    27241 aaaacgattt taattacaac aacaatttta gaaaggggtg tgacatttcc ccaaattgat
    27301 gtttttgttc ttgggtctca tcatcgtgtc tatagttccc aaagtcttgt tcaaattgct
    27361 ggacgagttg gaagatctat agacagacca gacggaacgt tatatttctt tcatgaaggt
    27421 atcagtaaag caatgctact agcaaaaaaa gagattaaag aaatgaatta taaagggtat
    27481 tcgcatgatc tgtctactat gtcaacaaat tagtcaaaca ccaataagta ttacagaaat
    27541 catcttttta aggcgtatct cttcaccgat ttgtcaacaa tgtcaaaaaa gctttcaaaa
    27601 gataggaaaa agtgtttgtg cgacatgttg tgcaaactca gatataatag cttgtcaaga
    27661 ttgtctaaaa tgggaaaaca aaggatacaa tgtaaatcat agaagcttat attgttataa
    27721 tgctgctatg aaagcatact tcagtcaata taagtttcaa ggagactatt tattaagaaa
    27781 agtttttgca gtagaacttg ccgatgttat caccaagtac tataaaggct atatcccagt
    27841 cccggttcct gtaagtctcg gttgttttcg agaaagacaa tttaatcaag tgagcgctat
    27901 tcttgaggca gctaatgtta gctacctttc tctttttgaa aagctagata atactcacca
    27961 atcttccaga acaaaaaaag agagattatt agtagaaaaa tcttatcgac tactaaaagt
    28021 atcaaacatt cctgataaaa tccttatagt agatgatatt tatactactg gtagtacaat
    28081 tatcgctctt agaaaacaat tggctaaagt agcaaatagt gacattaaaa gtttgtcaat
    28141 tgcacgttaa aacttgcaaa ttttcatgaa agcagtataa tagtattaaa gataaagtaa
    28201 gcgcttcaag cgcgagaaag aggtatccta tgattaagtt cagtattcgt ggagaaaata
    28261 tcgaagtgac agaagctatt cgcgattatg tagaatcaaa gcttactaaa atcgaaaaat
    28321 attttgctaa agaccaagaa atagatgcac gtgttaattt aaaagtttat cgggagagat
    28381 cttcaaaagt tgaggtaacg atcccattag attcagtaac ccttcgtgct gaagatgttt
    28441 cacaggatat gtatggctct attgatttag ttgttgataa gatcgaacgc caaattagaa
    28501 aaaataaaac caaaattgct aaaaaacatc gcgaaaaggt tccaactggt caagtcttta
    28561 caactgagtt tgaagctgaa gaagtcgatg agataccaga agtacaagtt gtacgtacta
    28621 aaaatgtgac attaaaacca atggatgttg aagaagcgcg tttacagatg gaactattgg
    28681 gacatgattt ctttatctac actgattctg aagatgatgc aacaaacatt ttataccgtc
    28741 gcgaagatgg aaacctagga ttaatagaag ctaaatagtt cacaactaaa acctacaggg
    28801 atgattatac gtattttaaa agtgggaaaa agctattcat gtttagaggt gacatgatga
    28861 aaatgagatt aagtgataga tacgacagtc attatgctaa caaaagtatc tattacagtt
    28921 cctctttaag taaatcttac gtaaaattag tttgaaaaaa gattagtccc aattctgggc
    28981 tagaaattct caaaaaatat gaaaaagcgc gttttcagaa tataaagcgc gttttttctt
    29041 tttccattta acaataaaaa actcatgacg aatatggtat cataagagaa tttttacttt
    29101 ttaaaaaaag gtcaaagact ttctgacacc aaataaaaat agaatttttt ctaactaaaa
    29161 ataatagcac aatctttggc agcgatccga attgtttttc cataaaagat ttaagcactt
    29221 acgtttgctt aaaatatagg ttatgtgacg tgcgtgcaag atcagattat aacaaaatag
    29281 ttccaaataa gcctatcact gagaaagcat cacagaaggg ttaaactttt tttgtattag
    29341 cgttttatca gtacaaccta acacttattt ttataaaaaa ataaaaaagc tattgacaat
    29401 cttatacaag ataacgtata ctaataaagc tgttgtaaat atggtaaagc aatagatgct
    29461 atcaggaaaa aatgtgaa
//
LOCUS       DATURV010000016        27847 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DATURV010000016 DATURV010000000
VERSION     DATURV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27847
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.016"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..155)
                     /locus_tag="VCF75_000691"
     CDS             complement(<1..155)
                     /locus_tag="VCF75_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HEP2546273.1"
                     /translation="MKQPLALTWEDQIRLFEKRGLIVKADDVEKIKHISYYRIKEFAK
                     PLEIKRQ"
     gene            complement(449..1666)
                     /locus_tag="VCF75_000692"
     CDS             complement(449..1666)
                     /locus_tag="VCF75_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEP2546274.1"
                     /translation="MTVDTEKAIAWMGLKEGRVSYSMDYRNGPDSYDCSSSVCSALIY
                     AGASNPGWLLNTEYMHDWLIQNGYQLIAENADWASLRGDVFIWGMRGQSAGAGGHTGI
                     FIDPDNIIHCNYARNNITIDNYNQTAVASGWMYSYAYRYVGGTDKCVGSKSIDELAQE
                     VLSGKHGSGEQRKISLGANYDAVQAKVNEMLKQPKVAEQSPAVKQDGDLMFNGAVLKK
                     SILDKILVKCKEHDILPSYAITVLHFEGLWGQSAVGRSDNNWGGMTWTGQGNRPSGIT
                     VTQGTARPVAEGGHYMHYESVDDFLTDWFYLLRAGGSYKVSGAKTFSEAVKGMFIVGG
                     AKYDYAASGYDNYIVGMSSRLKAIEQENGPINKYDQQADISAEQSDKIDVVIDSLEIT
                     INGVTYTATKKPI"
     gene            complement(1782..2009)
                     /locus_tag="VCF75_000693"
     CDS             complement(1782..2009)
                     /locus_tag="VCF75_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEP2546275.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQLGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALTYHEPKK"
     gene            complement(2006..2281)
                     /locus_tag="VCF75_000694"
     CDS             complement(2006..2281)
                     /locus_tag="VCF75_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546276.1"
                     /translation="MATELIFGVGGFILAIVTTYNIFNAKSIKHATDITLLQSEVEHL
                     KIVTRQNARRLEEHAEQNKTLITMTEQIKNLTDDVKDLKDMMRGEIK"
     gene            complement(2291..2908)
                     /locus_tag="VCF75_000695"
     CDS             complement(2291..2908)
                     /locus_tag="VCF75_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEP2546277.1"
                     /translation="MRNWKVTGKYPQFDSTGAVASTHIIITAEDGSVISQPIKQDLNS
                     TNDTEIIKATLEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQRMINLQTIHMLTSGGKIDSDIYKGMLELIEPAQKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEAFEYDKQTLEELESEAKVTVIKYADLVKQD"
     gene            complement(2911..3342)
                     /locus_tag="VCF75_000696"
     CDS             complement(2911..3342)
                     /locus_tag="VCF75_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEP2546278.1"
                     /translation="MDLTIKNKELNTLYSVLDKIKITNMRANRGRAKLLAKVVDKFKE
                     YAKDEGDLIDLYAQKDKDGKFVIDEHKNIKLADPTKLDEFSGLLNELADEEIVIKGGE
                     YSKRFIDFLNFLEECEDEFTSSEIILIDNILEQFEESKKGE"
     gene            complement(3351..5240)
                     /locus_tag="VCF75_000697"
     CDS             complement(3351..5240)
                     /locus_tag="VCF75_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEP2546279.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFTADGDTKSVKIANNRCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGTGNPTGLFKDLKEISLELTDTKNSKLWSKIKL
                     NNQGMIEEYYNGTIKSEIIKTAEGTQQRISSETDKKLALINETVSGIRREYQDADRQL
                     SSSYQAGIEGLKATMANDKIGLQAEIQTTARGLSQKYDNELRQLSAKITTTSSGTTEA
                     YENKLNGLRAEFTRSNQGTRTELESQISGLRAVQQTTASQISQEIRDRAGAVSRVQQD
                     LDSYQRRLQSAEGNYNSLRETVAGYERRISNQDNTISSNFTQLKTLIDQSVTLEKVQS
                     LLRQSGDSIMLAIKDKLPKSKMSGNEIISAINLNSHGVQIAGKNITLDGNTTVNGAFT
                     TKIANAIKIKADQIIAGVIDAAKIRVINLNASSIVGLDANFIKAKIGYAITDLLEGKV
                     IKARNGAMLIDLNTAKMDFNSDATINFNSKNNALVRKDGTHTAFVHFSNATPKGYTGS
                     ALYASIGITSSGDGVNSASSGRFAGLRSFRYAEGYQHTAAVDQTEIYGDSVLVVDDFN
                     ITRGFKFRPDKMAKMLDMNDLYAAVVALSRCWRHLSNVGWNPSHRNFINAVNSELNNY
                     VQKII"
     gene            complement(5251..5580)
                     /locus_tag="VCF75_000698"
     CDS             complement(5251..5580)
                     /locus_tag="VCF75_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546280.1"
                     /translation="MRGHNLLKDLNNIIINNKTIDRIICNNTIVYQRSWNLLFDGLLT
                     SAGKKLDIYPFYKIVINNAFFVKTPTTKNNTIFLAGHKILALQDSHFCFFRALEKSFF
                     VKIYGKN"
     gene            complement(5567..6790)
                     /locus_tag="VCF75_000699"
     CDS             complement(5567..6790)
                     /locus_tag="VCF75_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546281.1"
                     /translation="MTLEERIPIKVLFDRKDAAEWQKLNPVVDDGELVVELDTHRLKV
                     GDGKLTYNDLPYYEGPQGESITKVQLSENGDLSVWIGEKETKLGNIKGQKGDKGTSIT
                     DITKDGETLTIKLSDDTQKTFNIPNGQKGDRGKSVESARVDESGHLKLKIEEESELDL
                     GNVKGESGPKGDSITITGQQRVSEGVQLTFSDKTKVVIPKGEKGDTGDVNGINLEDYV
                     KKSELKNVGSADVKAINDFLGLSQKVFTSSYSYTDSLLKSYAKPSYSASWYVNESTVS
                     TKNGDKVLITIHNTTTQADNYLEVAVTYVGANYVTATSTGRLLTTPGEVKVVTKKQAK
                     EDYAAKKHKHEISDIAGLNERLSGYLRQADIQSQLNNIGKLKDTQTGQYLEVKVVDKG
                     QVPSNTSGMIVFERS"
     gene            complement(6787..8898)
                     /locus_tag="VCF75_000700"
     CDS             complement(6787..8898)
                     /locus_tag="VCF75_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP2546282.1"
                     /translation="MIYLFDRDEKLIKIIRKNACKSVIQKQTLTDTNYVSDLLTAELK
                     ALPDDILEQSEYMAVPTKEDKRKFHLFFIVRDETEGNATVLSGVQSGIEELRKAVAEN
                     KRPRKRDAREVIKYLLKDTNWHARYVAETGKSSTNFYYDSVFDSLLKVCSAWQLEMQF
                     FVEMNNNEIGARYIDFKKKIGVAEGRRVVYGHNALKILKQNEKAELITAVIGRGNGQE
                     VSSAEENEGGKAGYGRKITFKDVVWTKPNNPLDKPAGQLYLELPEATEEYGINIKGKK
                     VPKIGVVDIDSDDPEELLQRSYEYLVDHARPRAVFKTTSAYLKANIGDTVRVVRKDRN
                     LDYEVRVFEITWDRLSNKAIDIKLGDRLNASDSLKKAQLQSHLTDSVSDTVFDTLGQF
                     LKDELPSAGGFNTNFYSENEPDKSKVKIGDIWFKPDPEHEGHKIMLIWDGKNWVEVVR
                     SYNSDIAKTAVEEAIKEIEKLKQHQQELDERNQKELDEFRATLKNLALPEEAIKKITE
                     TIKVDDIPSIKQSFDDLKNKVSETSETSRLNAEILGNNGKTHYNKNLLVGDPNRVKTY
                     DQDYIELEANDGGFKRGETYTISFSQTCELLKKVAITLTQINNKGVKLVLTPTKAKMD
                     AQTFEVAKDKQSIEVYPLSYTAVLTGDWYKSKQIDLNASEVQELALDMSYKEIADAKG
                     ATIIGKQSDKPKIILDGRRDR"
     gene            complement(8895..9665)
                     /locus_tag="VCF75_000701"
     CDS             complement(8895..9665)
                     /locus_tag="VCF75_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEP2546283.1"
                     /translation="MYDYVNLKGGASVTARLPSDNMSVNGKPLNELIPGYRQLHVSGR
                     GIVSQEIETIAIPSRRGVYISNIQDKEREITVTFQLTSDNSEGLREAFAKLNKNLRGE
                     LSLTFADEKDFTYKGFLKSAPDDSEQSLTLVSSFTLLIPDPYKKGALKTSRGPITLTH
                     ADKVLPNKIVLQSTGGAEIEITAGSERMKLKGHYGSGDKITIEWLDDEVVIKHNNNLA
                     LSNLLHLSVPEEFYVKNGDTIQATNAAVESVEWRDEQL"
     gene            complement(9665..12664)
                     /locus_tag="VCF75_000702"
     CDS             complement(9665..12664)
                     /locus_tag="VCF75_000702"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEP2546284.1"
                     /translation="MAGVADFTLTALIKADASGFRAGVQDAKNALNELQKTTGSRLQR
                     IGDTFGGVGKALTAGLTLPLTALGGASLKAFSDYDTALIGVRKTTDISGDALNRFSKQ
                     IMSLSREMPNSAVEIANLAEVAGQLGIRTDEGGKHLINFSRIAAQMGTATNMSSEQAA
                     NAMARLANITQMPQTQFHRLGSTIVALGNKMATTESEISEMALRLAGTGHQVGLTEAQ
                     IAGLAAGMSSVGINAEAGGSAFSRVMQKMNTDVLSGGEGLQKFASVAGKTAEEFAQTW
                     RKNPQEAIVDFVKGLGKIKESGGDVTETLKDLGINSVREIDTLARLSGAGDLLAKSFN
                     IANKAWADGTALQKEADAASQSFANQLKLLKNALFEAGTVIGKHLAPRIGKLAEGIKK
                     ITQAFSDLDPKVQDGIISFGIFLATIGPVFLVINKIITGIRMLATAFGFIKGLVTAFK
                     TAGTALTGFKAVIAALGGPITLIIAGIMALVGVLTYLWTTNEGFRNAVTGIWEAVKQV
                     FSTAWEYIKNAWAAAPSFFSNLWETIKNGASSIWDNVKTVWASVVDFLKAVWQGLADF
                     FSPLWEGIKSSALGVWESFVTGMIPIVDAFKNLWSSLTEFFTALGEQIKPIWEDIKQF
                     FTDTWNIIVTIFQSALAILTPVVQAGFEIIKLQITLVWEFIKTYIQLAWVIISNTVQV
                     ALTTLAAIITAGFEIIKTVIQTMWEVIKILVQSAWTVITTIVSTAINVVAEIIRTVTA
                     VIQGDWQAAWEAIKSIFEVAWNAIKTIGETLFNALKSVIETILNALKSIWDSVWNAVK
                     ATAESVWNAIKAIVSGAMDAMKNAVSNGINAVKNFIVNGWNSVVGFLSSINLFSAGSA
                     IMQSFLSGLKSMWGAITSFVGGIASWIKSHKGPISYDRRLLIPAGKAIMSGLNNGLSN
                     GFGQVKNNVSGMADSISKLVDGNSLFDADMSSTISGAVATGVEVDLGHQAKPLELNLE
                     LGNRAYGLFVDDITDRQRATARLTEVY"
     gene            complement(12674..12982)
                     /locus_tag="VCF75_000703"
     CDS             complement(12674..12982)
                     /locus_tag="VCF75_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546285.1"
                     /translation="MAIAMARFGLSLLEAKRLTWHDYCIYDLAHQVKMQEEETMLAKQ
                     AWFNQAVKATKGTGKNVRSAYKSFKDFYDAGKAFDAIFDTKPKKPKLSIADLNRVINE
                     "
     gene            complement(13003..13371)
                     /locus_tag="VCF75_000704"
     CDS             complement(13003..13371)
                     /locus_tag="VCF75_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546286.1"
                     /translation="MEITVGKKTYDLVFGFDFLKYANKHRAIEMDGVGIGAGSMTKIE
                     AGSAFKDPEVLLFILKCATSTEKTKPSNEELEKFIEEAIVNGTYIGFYDEVVAEIKKD
                     AVLQQAIAGNQVIEAVTKAK"
     gene            complement(13384..13944)
                     /locus_tag="VCF75_000705"
     CDS             complement(13384..13944)
                     /locus_tag="VCF75_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEP2546287.1"
                     /translation="MAELIQGKSFLLFFRRLDDVETKDAAKLRFQTEHSIKMEKETEA
                     TKTKDGIINSLSDGENTIDIKSFAYTSDESTTAIWEELRKYFKDGDVVEIWQVDLTSK
                     DSGHQKYKAEYFQGYFTSFEISAPADGQVELSMTYAINGNGVEGVDELTSEQKQAVSD
                     VLYRYKSMARGGAEPEETKKENSFEM"
     gene            complement(13945..14331)
                     /locus_tag="VCF75_000706"
     CDS             complement(13945..14331)
                     /locus_tag="VCF75_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546288.1"
                     /translation="MTPNNAVFRQIFKECLAVTKNTYDYLPNANAKYPFAYIGESSNT
                     PSNNSEMMGVIRQTIHVYGELKKRSEIDNVVTNLHDKIISLTSAYGYNFRVDRINEQT
                     LPDNSDTTPLLHTVLDVYLTYTKKGN"
     gene            complement(14328..14684)
                     /locus_tag="VCF75_000707"
     CDS             complement(14328..14684)
                     /locus_tag="VCF75_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEP2546289.1"
                     /translation="MPSVTIEWKGIEKAFSKLVNASGNIDRLTDAVVKNHAEKAKAEA
                     QRIAPYDTGFLHDEIYTHYPAKASAEIVSNAGYSGFLEYGTRKMPAQPFLRPAIESIL
                     PDLEKDYNSVIRRAFK"
     gene            complement(14684..14980)
                     /locus_tag="VCF75_000708"
     CDS             complement(14684..14980)
                     /locus_tag="VCF75_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546290.1"
                     /translation="MRYADRVTFVKTTDEQYDPDLGEYTHTETISETKPCFAMEMGAE
                     KSVQIFGDYQKDRKVIYLKQPYTKAFDYCEYEGRRYKAQANKLGAIVFYLEEVL"
     gene            complement(14977..15330)
                     /locus_tag="VCF75_000709"
     CDS             complement(14977..15330)
                     /locus_tag="VCF75_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEP2546291.1"
                     /translation="MQEINTDSIIANVKLDLGILDSQQDDLLNMLLKRVTDHFKAKYG
                     VVKIDSAFSFVLEDCLIARFNRRGAERAKSESVEGHTTTYYDFLDEFEPYDNMIMAKL
                     DLIKDKSRKGGLYFL"
     gene            complement(15344..15586)
                     /locus_tag="VCF75_000710"
     CDS             complement(15344..15586)
                     /locus_tag="VCF75_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546292.1"
                     /translation="MPRVIRAFKDKVTKVVYEVGDIYSGDRVEFLTEGGVLEPSVDFD
                     KLKVSEIKSKLDELSIEYDAKLKKSELLELLKQTIG"
     gene            complement(15596..16669)
                     /locus_tag="VCF75_000711"
     CDS             complement(15596..16669)
                     /locus_tag="VCF75_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEP2546293.1"
                     /translation="MAYIHEIITSENIKGFYDKQKGEAELTLGEKAFPSKQQLGLKLS
                     FIKGAAGRPVTLKAAAFDTKVPLRDRMSVELIDEEMPFFKEAMLVKEADRQQLNMLAQ
                     TKNQELIDTILASIFNDKVTLISAAKARIEAMRMEVLSSGKIHIASNGVMKDIDYGMT
                     EEQTTKAKNKWSDANQATPLADITTAIEAMAERGYAPEAIIMNSKTFSLIKNADNTVE
                     VVKPMAPQGSAVTKNDLNTYLEDELQVKVILKDGMFVGDDGKARKYFPDGVATLVPNG
                     SLGSTVFGTTPEQSDLMGGQATDAEVSIVENGIAVTTTKTTDPVNVQTKVSMIVLPSF
                     ERLAEVQIINANGETKKENSFEM"
     gene            complement(16669..17049)
                     /locus_tag="VCF75_000712"
     CDS             complement(16669..17049)
                     /locus_tag="VCF75_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEP2546294.1"
                     /translation="MNKRKVTTSKEILHNLPYEAISVTLDSSELDEKIIKAGAVLAGK
                     EKSVFEDRTQKVKEVKNGEISSENNICGILLTDVDLTNGDAVGSCVYRGTINADKLAD
                     PSIAENYKDLKEKLPHIQFIEGGK"
     gene            complement(17059..17592)
                     /locus_tag="VCF75_000713"
     CDS             complement(17059..17592)
                     /locus_tag="VCF75_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEP2546295.1"
                     /translation="MAEETQTVETVEEQVVPEAKQPQDEKKYTDADVDAIIDKKFAKW
                     KSEQEAEKSEAKKMAKMNEKEKADYEKQKLLDELQELKNDKTRNELTAVARQMFAESE
                     INVNDDVLGLVVTLDAEQTKANVTTLANAFAKVIADDRKALVRQTTPSTGGGLSKQTN
                     YGANLASKAAQQSTKLF"
     gene            complement(17822..18091)
                     /locus_tag="VCF75_000714"
     CDS             complement(17822..18091)
                     /locus_tag="VCF75_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546296.1"
                     /translation="MNKRIKKKRKLEQVIVLLIAENAMQAEAIKNQNRQIAELRAIIQ
                     QNVQATNNELAAVKGVCFDNQAAITNIAIDVNYIKKNYKRKWGKK"
     gene            complement(18084..18263)
                     /locus_tag="VCF75_000715"
     CDS             complement(18084..18263)
                     /locus_tag="VCF75_000715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546297.1"
                     /translation="MNKISLNIEMTWENKDEFQQIMNKVNETKEAYKKALEAAGNFVP
                     EMSCATKLNKGEGNE"
     gene            complement(18278..19198)
                     /locus_tag="VCF75_000716"
     CDS             complement(18278..19198)
                     /locus_tag="VCF75_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEP2546298.1"
                     /translation="MKSTQKQRNEAYNRERKAMDAWVKADLENEAMLKQLYTQSKAQI
                     EANIDRFYMRYADKHGLDKRTVKKLADSFDVTAYQNMAKEAVANRDFSKEANLWLSVY
                     NLKMKVSREELLRAELNYELLKMHTKIDNQIQLAREKIALDEMKRQAGILGGQGSVAK
                     RLKGILNADFYGKNFSERVWGNNGLYHETQKSLFSSLSRIATDMQGYKKEKERLMRRF
                     DVAEHEALRLLKTESARIRSDVQLDAYKKNGFTHYIFVCEPGACQICTPYDKERFELS
                     KGATAITMPPLHPNCRCSTYGEVDYGSVIK"
     gene            complement(19170..20672)
                     /locus_tag="VCF75_000717"
     CDS             complement(19170..20672)
                     /locus_tag="VCF75_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP2546299.1"
                     /translation="MYTESFRDSTGKTKTLEFRFHREARMRYQAESLESLLTEKYKLL
                     REMIEHHDKVQKPRIQELLDYAEGNNHTISEMGRRKDDDMADVRAVHNYGKYISTLKQ
                     GYLVGNPIRVEYIDGTEQQQDLLKDLSVKNNFHQLNRRLVKDLSKVGRAFELIYRSMD
                     DKTEVVRLDPREVFVIYQNNLEQSSLAGVRYYNKNQLDGTTKIVELYTDNKILKFEYD
                     GDLTPIGEASSHAFGSVPITEYLNTDDGMGDYETELSLIDLYDAAQSDTANYMQDLSD
                     AILAIIGRVSFPGYVDTAEKAIEYLRKMRKARLLNLEPPVDQDGREGSVDAKYLYKQY
                     DVQGTEAYKNRIVSDIHKFTNTPDMTDTKFAGQQSGEALKWKVFGLDQERVDMQALFE
                     QSLKRRYKLIARVSQLLKEIDDFDISKLKITFTPNLPKSLQEKIEAFKALGGELSQGT
                     IMAITDIVEDARKEFENIQSERREEESNNPSLSDFDLGVLRREINTETTK"
     gene            complement(20684..21991)
                     /locus_tag="VCF75_000718"
     CDS             complement(20684..21991)
                     /locus_tag="VCF75_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEP2546300.1"
                     /translation="MKTTYQTKPKLEIKVDLPKTIGIGYGAFWRSKNFYRVVKGSRGS
                     KKSKTTALNFIVRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNQLKVTHLFKFNES
                     LPEITVKATGQKILFRGLDDELKITSITVDAGALCWAWFEEAYQIETEDKFSTVVESI
                     RGTLDVPDFFKQITVTFNPWSERHWLKRVFFDEETKRDDTFSDTTTFRVNEWLDEVDR
                     KRYLDLYKTNPRRARIVCDGEWGVAEGLVFDNFEVIDFDIDKIVSKHDTSSGMDFGFT
                     QDPTTLICVAVDLESKELWLYNEHYQKAMLTDDIVKMIRDKNMHKSYIAADSAEKRLI
                     AEIRSKGISGIVPSLKGKGSIMQGIQFIQGFKIHIHPSCEHTIEEFNTYTFKQDKEGN
                     WLNEPIDKNNHIVDAIRYALEKYHIARKQSTNTFNILRQGLKY"
     gene            complement(21969..22451)
                     /locus_tag="VCF75_000719"
     CDS             complement(21969..22451)
                     /locus_tag="VCF75_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEP2546301.1"
                     /translation="MDWRWWKIAYEELTEKQQRFVDKYITTFNATESAKQAGYSEKSA
                     YSQGQRLLKNVEIQKAMKERFLEAKDTKGDRIQDVAETLEQDTSIARGEIQISEFKET
                     DMLTGQVVIHTKREYTPSHEEQGRARDRIYKVNGAYSEKRELEHSGTVVFVNEDDIPD
                     "
     gene            22511..22768
                     /locus_tag="VCF75_000720"
     CDS             22511..22768
                     /locus_tag="VCF75_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546302.1"
                     /translation="MLSLLPTPFWDTSIYRTGTVGIEPTHLLLALRKEFSDLAIVPKQ
                     GATLTVFQSRFMFQRFRRAKTSHKRSSTDLARIERATALNI"
     gene            complement(23343..23780)
                     /locus_tag="VCF75_000721"
     CDS             complement(23343..23780)
                     /locus_tag="VCF75_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP2546303.1"
                     /translation="MGNIPTTKANNFLEELKTIPHLIETLERDANLMSRSLVKSPQWS
                     DMRVSGGVKQSQEDKNIKMLHMVSYYSDQIERLKDRRQEMANLIVQSMGICESHVLLT
                     TYLDCDGDYERARERLNIGNRNKYFMFVRRGKESLELILKNTN"
     gene            complement(24047..24241)
                     /locus_tag="VCF75_000722"
                     /pseudo
     CDS             complement(24047..24241)
                     /locus_tag="VCF75_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
     gene            complement(24207..24533)
                     /locus_tag="VCF75_000723"
     CDS             complement(24207..24533)
                     /locus_tag="VCF75_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546304.1"
                     /translation="MIPKFRAFNKKIQKMYSIDGFKSSERKIYRCSLADDEFRSGRLE
                     TFHFVEDNLGDYILMQSTGLFDKNGVEIFEGDVVKLQYTITSDLEFFKVNQFRGGAWR
                     LFNKTS"
     gene            complement(24543..24812)
                     /locus_tag="VCF75_000724"
     CDS             complement(24543..24812)
                     /locus_tag="VCF75_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546305.1"
                     /translation="MKKPNRYPYSKSKFNGCIYQLHAASFKDEQYVEDLKSCGIHYQI
                     TKIGYFPDIFIKIDNLEQLQILIDKTGHDLILSKDQIWIYDDYME"
     gene            complement(24809..24991)
                     /locus_tag="VCF75_000725"
     CDS             complement(24809..24991)
                     /locus_tag="VCF75_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546306.1"
                     /translation="MVKHVVRVYNKGITATYAIYDKNLFQEHEFVTKIEAMQFIRKLE
                     LANDKRATEYFMREAE"
     gene            complement(24994..25218)
                     /locus_tag="VCF75_000726"
     CDS             complement(24994..25218)
                     /locus_tag="VCF75_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546307.1"
                     /translation="MKIDYINFFERVVPDWMRESNVKMQEVGFNTEAYWIWANQSIVT
                     ICETYGNDSLINGQFHLIWEWLESKTKESS"
     gene            complement(25215..25610)
                     /locus_tag="VCF75_000727"
     CDS             complement(25215..25610)
                     /locus_tag="VCF75_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEP2546308.1"
                     /translation="MIDFFIPMKKIPTTTHQQKKVRVVNGKPQFYEPDSLKEARAMFM
                     SKFAPHAPEKKMTGPLRLTVKWLFPKIKKSVDGQYKDTKPDTDNLNKLPKDCMTALGF
                     WNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            complement(25603..25785)
                     /locus_tag="VCF75_000728"
     CDS             complement(25603..25785)
                     /locus_tag="VCF75_000728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546309.1"
                     /translation="MQIEFFKNGKSDIELEYLKNEVNGFINDLREDGIKCLDIQFIFD
                     EDEIIVFVKYEGLIDD"
     gene            complement(26078..>27847)
                     /locus_tag="VCF75_000729"
     CDS             complement(26078..>27847)
                     /locus_tag="VCF75_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2546310.1"
                     /translation="TDEGVVYKPTKGDYARTAGELIQELQKYGNDIGAVLGDYKEEAG
                     AWIRFNPLDGNGVKNDNVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSL
                     HAIVKVDARDYQEYRKRVDYIYQICKKNGLDIDTQNRNPSRLSRMPGIMRNGHKQFLI
                     DTNIGKTNYEEWYQWIEDLNDDLPEPETLADEWDNLPELAPELIKGVLRQGHKMLIAG
                     PSKAGKSFALIELSIALAEGQKWLGWQCEQGKVLYVNLELDRPSALHRFKDVYDAMGL
                     PPTNVANIDIWNLRGKTVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQM
                     AHFTNQFDKVATELGCSVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELE
                     LSDDLIKVRADKAANKIYQRALQERNLGYYQQYVSLDDLESNAQMRQHFEKAISDVLV
                     RKIYTDEIKQAVHAINISTGWRVEGTLREFAKFQPVNMWFSYPVHEVDTTGVLADIQL
                     EESKPNWQKAKEGRKSKKEKAEERKQKIDTAYSALFDGFTPVSLSELQEYLDSTEKTV
                     RNHLKEHGGFDIKNGIVYQKKTE"
BASE COUNT         7294 a         6165 c         4808 g         9580 t
ORIGIN      
        1 tcttgtcgct ttatttcaag tggttttgca aattctttaa ttctataata actgatgtgt
       61 ttgattttct ctacatcatc agctttaaca attaatccac gtttttcaaa taacctaatt
      121 tgatcttccc atgtcaaagc taaaggttgt ttcataactc tcctaacgat ataaaaaaag
      181 ccccatatca gaacgtatct gcctttaaag gaatgggggg tttgtcttgt taagataatt
      241 atactttttt taaaaataat gtcaagacac aagatcgtgt cttgaaactt ataaattcaa
      301 gcgaacacaa catattgaat aattgtatat aaaaatacat ttttaacaca atatattgta
      361 aaaattttgt tgcattcact attaatacca aaaaacagcc cagaaattaa tctgagcggt
      421 tttgtcttat cttaaggagc tttacctcct atattggttt tttagttgcg gtgtaggtaa
      481 caccattaat agtaatctca aggctgtcaa taaccacatc aatcttgtca gactgctcgg
      541 cactgatgtc ggcctgttgg tcatacttgt taattggtcc attttcctgc tcgattgctt
      601 ttaatctgct agacatgcct acaatataat tatcataacc actagctgca tagtcatatt
      661 tagcgccacc aacgataaac atgcccttta cagcctcact aaaagtctta gctcctgaaa
      721 ccttgtaaga gccaccagct cttaacagat agaaccaatc tgtcaaaaag tcatcaacac
      781 tttcgtagtg catgtagtgg ccgccctcag caactggtcg agccgtgcct tgggtaaccg
      841 ttataccgct tggacgatta ccttggccag tccatgtcat gcctccccag ttattatcag
      901 agcgacctac tgcagattga ccccaaagcc cctcaaaatg caacacagta atggcataac
      961 ttggcaagat gtcatgctct ttacacttaa ctaggatttt atctaagata gactttttaa
     1021 gcacagcacc attaaacatt aagtctccgt cttgtttgac agcagggctt tgctcggcta
     1081 cttttggctg tttgagcatc tcgttgactt ttgcttgcac ggcatcatag ttagcaccaa
     1141 gtgagatttt gcgctgctcg ccactaccat gtttgccaga taggacctct tgagctagct
     1201 catcaatgct tttgctaccg acacatttgt cggtacctcc gacgtaacga taagcgtaag
     1261 agtacatcca accgctggct acagctgtct gattataatt gtcgattgta atgttattgc
     1321 gagcgtaatt acaatggata atgttatctg gatcaataaa aatacccgtg tgaccaccag
     1381 cgccagccga ttgaccgcgc attccccaaa taaaaacatc accacgtagg ctagcccaat
     1441 ccgcattctc ggcaatcaac tgataaccgt tttgaattag ccaatcatgc atgtactctg
     1501 tattgagtaa ccaaccagga ttactagcgc cagcgtaaat caatgcgcta caaacagagc
     1561 tagagcagtc atagctatcg gggccattgc gataatccat agaatagctg acacgtccct
     1621 ctttaagacc catccaagcg atggcttttt cagtatctac tgtcattctt cttcctcctc
     1681 aaaaaacgga taaaagataa gagcaatcac agataatggc acatacagta ttgcgattgc
     1741 tagtattaac gctaatcgtg tgattgctcg catggctcct cctatttttt gggctcatgg
     1801 taagtcaatg cttgctcact atctgacagg cctttcgtgg ttggatctgt gacaacaccg
     1861 agcaatacca aaagcgttac agctgtgttt gcaatatctg cgatgtttga tggtagttta
     1921 atacctaatt gctgtgctaa caaaaagata gctcccaaaa tagccatcaa ggttactttg
     1981 ttttgtagtc gtaattttaa attgatcatt tgatttctcc tctcatcata tcttttaaat
     2041 ctttaacatc atctgttaaa tttttaattt gctctgtcat tgtaatcaac gttttatttt
     2101 gctcagcgtg ctcttcaagc cgccgagcat tttggcgtgt aacaattttt aaatgctcta
     2161 cctcagattg caacaaagta atatctgtcg catgcttgat ggattttgca ttaaaaatat
     2221 tgtaagtcgt gacgatagct aaaataaagc caccaacgcc aaatatcaac tctgttgcca
     2281 taaaccacct ctaatcttgt ttaaccaaat cagcgtactt gataactgtt actttggctt
     2341 ctgactctag ctcctctaag gtttgtttgt catactcaaa tgcttcgtta acgtgtacga
     2401 agactaggtt accttcacca gcctctccgt cttcctcttt agtactgtcg accaccgtaa
     2461 agacatcata ggcttgatac tcaccttttt gggctggctc gattagctca agcatgcctt
     2521 tataaatgtc agaatcaatc ttgccgccgc tcgttaacat gtggatggtt tgcaagttaa
     2581 tcattcgctg tgtacgctct gcggacacct tagctaatcc tgcggctgtt tgggctgttt
     2641 tagcggtctt agcggtttcc tgtgagattt tttcaaggtc gtctactttt tgcacggctt
     2701 cgcccattgc aatttcaacg tattcagatt ttttaaattc ttccaaagta gctttgataa
     2761 tctctgtgtc attagttgag tttaagtcct gcttgattgg ttgagagatg actgagccat
     2821 cttctgctgt gataataata tgtgtgcttg cgactgctcc tgtgctgtca aattgtgggt
     2881 attttcctgt cactttccaa tttctcatgg ttattctcct tttttacttt cttcaaattg
     2941 ttccaaaatg ttgtcgataa gaatgatttc agatgatgta aattcatctt cacactcttc
     3001 taaaaagttt aaaaagtcaa taaatcgctt ggagtactca ccccctttaa tcacaatttc
     3061 ttcatcagct agctcgttga gtaggccgct gaactcgtcg agtttagtgg ggtctgctag
     3121 cttgatgttt ttgtgctcat caatgacaaa cttgccatct ttgtcttttt gagcatacag
     3181 atcaataagg tcaccctcat ccttggcgta ctctttgaat ttatctacta cttttgcgag
     3241 tagcttagca cgtccgcgat tagcacgcat attagtgatt ttgattttgt ctagtacact
     3301 atatagtgta ttgagttctt tattttttat tgttaaatcc attgtttctc ctaaataatt
     3361 ttttgcacat agttattcag ctcactatta acagcgttga taaagtttct gtgagacgga
     3421 ttccatccaa cattagacaa atgcctccaa cacctactaa gcgctactac agccgcatac
     3481 aagtcgttca tgtcaagcat ttttgccatc ttgtctggtc taaacttaaa tcctcgagta
     3541 atattaaaat catccacaac taaaacacta tctccgtaaa tctctgtttg gtcgactgcc
     3601 gcagtgtgtt gatagccttc tgcgtaccta aatgacctta gccctgcaaa acgaccggaa
     3661 gaagccgagt taacaccgtc accagatgag gttatcccga tcgatgcata caacgctgac
     3721 cctgtataac ctttgggcgt cgcattacta aaatgtacaa aggcagtatg tgtgccatct
     3781 ttacgtacta aggcattgtt tttgctatta aaattaattg tggcatcgct attaaagtcc
     3841 atcttagctg tatttaagtc gataagcatc gctccattac gagccttaat gaccttaccc
     3901 tcgagcaaat cagtgatagc atagccaatt ttagctttga taaagttagc gtctaaacca
     3961 acgatactgc tggcgtttag attaatcact ctaatcttag cagcgtcaat cactcctgcg
     4021 ataatctggt cagccttgat tttgatagcg ttggcaatct ttgtggtaaa agcgccgttg
     4081 acagtggtat tgccatcaag agtgatgttt ttaccagcta tttgcacacc gtgggagttt
     4141 aggttaatcg ctgagattat ctcattacca gacatcttgc tcttaggcaa cttgtcctta
     4201 atcgcgagca tgatactatc accagattgc cgcaagagcg actggacctt ctccaaggtc
     4261 acagactgat ctatcaaagt ctttagctga gtaaagttag aggagatagt gttatcctga
     4321 ttggatatcc tgcgctcata acccgctaca gtctctctca aactgttgta attaccctct
     4381 gcggactgta atcgtcgttg gtaactgtct aagtcttgct gcacacgact gacagcacct
     4441 gccctgtctc taatctcttg tgaaatttgg ctagcggttg tctgttgtac cgctcttagt
     4501 ccgctaattt gtgactcgag ctctgtcctc gtgccttgat ttgagcgagt aaactcagca
     4561 cgtaagccat tgagtttgtt ttcgtaggcc tctgtagtgc cgcttgaggt tgttgtgatc
     4621 ttagccgata actgtcttag ctcgttatca tacttttgtg ataatcctcg agcggttgtt
     4681 tgtatctcag cttgtaaacc gattttatca ttggccattg tggcttttag cccctcaata
     4741 ccagcttggt agcttgacga tagctgtcta tctgcatctt ggtactcacg tctaatgcct
     4801 gagactgtct cgttgataag cgcaagtttt ttatcggtct cgctactaat acgttgctgc
     4861 gtgccctctg cggttttgat aatctcagat tttattgtac cgttgtaata ctcttcaatc
     4921 atcccttgat tattaagctt gatttttgac caaagtttgg agtttttagt atctgttaac
     4981 tcgaggctaa tctctttgag gtctttaaat agtccagtcg gattacctgt cccctcgaca
     5041 accacgggcg ccacgtagtt agttggttta ccccctcgct caatcatgag ctggttaaag
     5101 cgagttgtac cgatacatct gttattggct attttaacgc tttttgtgtc accatcagcc
     5161 gtaaatgtat aataagcacg tccatctgag ccgattgtta aatttgactc gtctattaaa
     5221 agtgttgggt ctctactcaa atgttacctc ctaattttta ccgtaaattt tgacaaaaaa
     5281 acttttttct aatgctctaa aaaaacaaaa atgactatct tgtaatgcta gtattttatg
     5341 tcctgctaaa aaaatcgtat tgttttttgt tgtgggggtt ttaacaaaaa atgcattatt
     5401 gattacgatt ttataaaaag gataaatatc caattttttc ccagcgcttg tcagcaaacc
     5461 gtcaaaaagc agattccacg accgctgata gacaatcgta ttattacaaa taatacgatc
     5521 aatcgttttg ttattaataa tgatattatt taaatctttt aacaaattat gacctctcaa
     5581 acacgatcat gccactggta ttgctaggca cctgtccttt atcaaccact ttaacctcaa
     5641 gatattgtcc ggtttgcgtg tcttttagct taccgatatt attaagctgt gactgtatat
     5701 cagcttgtct gagatagcca gacaagcgct cattaagacc agctatgtca ctaatctcat
     5761 gcttatgttt tttagcagca taatcttctt ttgcttgctt ttttgtcact actttgactt
     5821 caccaggagt agtcagtaag cgacctgtgg aagtagcagt cacgtagtta gcaccaacat
     5881 aagtcaccgc tacctccaaa taattgtctg cttgggtggt agtgttatgt attgtaatca
     5941 atactttgtc accattttta gtagaaactg tagattcgtt gacataccaa ctcgccgaat
     6001 agctgggttt tgcataactt tttagtaagc tatctgtata actatagctg cttgtaaaca
     6061 ccttttgaga tagccctaaa aagtcattaa tagctttaac atctgcggaa ccgacgtttt
     6121 taagttcaga ttttttgaca taatcttcca gattgatgcc gttgacatca ccagtgtcgc
     6181 ctttttctcc tttcggaata acgaccttgg ttttatcact aaaggtcagc tgtacgccct
     6241 ctgagacacg ttgttgacct gtgatagtga tactatcacc tttaggtcct gactcacctt
     6301 taacgtttcc aagatctaac tctgattcct cttcaatttt tagttttaaa tgaccggatt
     6361 catcaacccg agcactctca acgctcttac ctctatcccc tttttggcca ttggggatat
     6421 taaaggtttt ttgagtgtcg tctgatagtt tgatagtgag cgtctcacca tctttggtga
     6481 tgtcagtaat acttgtacct ttgtcaccct tttgaccttt gatatttccg agcttagtct
     6541 ctttttcccc aatccatacc gataagtcac cattttcgga tagctgtacc ttagtgatag
     6601 attctccttg cggaccttcg tagtaaggca ggtcattgta agtcaatttg ccatcaccga
     6661 cttttagcct gtgagtgtct agctcaacaa ctagttcacc atcatcaaca acaggattta
     6721 atttttgcca ctcggcagca tccttgcggt caaataagac tttaattggt attctttctt
     6781 ctagcgtcat ctgtccctcc ttccgtctaa aataattttt ggtttatcgg actgtttgcc
     6841 gataatagtt gcacctttgg catctgcaat ctctttatag ctcatatcca gggccaattc
     6901 ctgcacctcc gacgcgttta aatctatctg cttagattta taccagtcgc cagttaaaac
     6961 agccgtgtag ctcaaaggat aaacctctat agactgttta tccttagcga cctcaaacgt
     7021 ctgtgcatcc atttttgctt tggttggtgt cagtactaac ttaactccct tgttattaat
     7081 ttgcgtcaat gtgatagcca cttttttgag tagctcacac gtttgactaa agctaatcgt
     7141 gtacgtctcg ccacgtttaa aaccaccgtc attagcttca agctcgatat agtcttggtc
     7201 ataggttttt acacggttag ggtcgccaac caagaggttt ttgttgtagt gggtcttacc
     7261 gttgttacct aaaatttcgg cgtttagacg ggacgtctcg ctcgtttcac tgactttgtt
     7321 ttttaggtca tcaaagcttt gtttaataga cgggatgtca tcaactttga tagtttcggt
     7381 tattttcttg atcgcttcct ctggtaacgc taggtttttg agggtggctc ggaattcatc
     7441 gagttctttt tggttacgtt cgtctagttc ttgctgatgt tgtttgagct tttcgatttc
     7501 tttgattgct tcttcaacag ccgttttagc gatatcggag ttataacttc taacgacttc
     7561 gacccagttt ttgccgtccc aaataagcat gattttatga ccttcatgtt ctgggtcggg
     7621 cttaaaccag atatccccta tttttacctt gcttttatca ggttcgtttt cgctataaaa
     7681 gttggtatta aagccacctg cgctaggcaa ttcatccttt aaaaattgtc ccagagtatc
     7741 aaaaacggta tcagaaacac tatctgtcaa atgtgactgt aattgagctt tttttaggct
     7801 gtcagaagca tttaacctat cgcctaattt aatatctatc gctttattgc ttagtctgtc
     7861 ccaagtgatt tcgaaaactc taacttcgta gtcaagattt cggtcttttc ggacaactct
     7921 cacagtatcg ccaatatttg cttttaagta agccgaggtt gttttaaaaa cagctcttgg
     7981 tcttgcgtga tcaactaaat actcatacga acgttgaagc aactcttctg ggtcatcaga
     8041 atcaatatca acaacaccaa tcttaggtac tttttttcct ttgatgttta ttccatactc
     8101 ttccgtagct tctggtaatt ctaagtacag ctggccggcc ggtttatcta gtggattgtt
     8161 tggtttagtc caaaccacat ctttgaatgt aattttccga ccgtatccgg ctttcccacc
     8221 ttcattttct tcagcagagg acacttcttg accgtttcct cggccaatga cagctgtaat
     8281 taattccgct ttttcgtttt gtttaagtat ctttaaggcg ttgtgtccgt aaacaacacg
     8341 acgtccctcc gctacaccga ttttcttttt aaaatcaata tacctagcac ctatttcatt
     8401 attgttcatc tcgacaaaaa actgcatttc cagttgccag gctgagcaaa cttttaacaa
     8461 agaatcaaac acagaatcat aataaaaatt agtagatgat tttcctgttt cagcaacata
     8521 tcgagcgtgc caattagtgt ctttaagcaa atatttgata acttctcttg catctctttt
     8581 tctaggcctt ttattttctg ctactgcttt cctaagttcc tcaatacctg attggacacc
     8641 gctcaaaacc gttgcgttgc cttcggtttc atcacgcaca ataaaaaaga ggtgaaactt
     8701 tcgtttgtcc tcttttgtcg gcactgccat atattcagac tgctctaaaa tatcgtcagg
     8761 caacgctttt aactcagccg ttaataagtc agatacataa ttagtatctg taagcgtctg
     8821 tttttggatg actgacttac aagcgttttt ccggatgatt ttgattagtt tttcatctct
     8881 gtcaaaaaga taaatcatag ttgttcatcc ctccattcaa cgctttcaac agcagcgttt
     8941 gttgcttgga tggtatcacc gttttttaca taaaattctt ctggcacaga cagatgaagg
     9001 agatttgaaa gagcaagatt attgttatgt ttaataacaa cttcgtcgtc taaccattct
     9061 atagtgattt tatctccgct gccataatgc cctttaagct tcatcctttc actgccggct
     9121 gttatttcaa tctctgcgcc tccagttgac tgtaaaacaa ttttatttgg aagcaccttg
     9181 tcagcgtgcg ttaaggttat aggccctcta gatgtcttta aagctccttt tttgtatgga
     9241 tctggaatca acaatgtaaa gctagaaacg agcgttagag actgttcgga gtcgtcagga
     9301 gcagatttta aaaacccttt ataagtaaag tctttttcat cagcaaaagt caaagataat
     9361 tcacctctta gatttttatt cagttttgca aaagcttccc ttaaaccctc tgaattgtca
     9421 gatgtcagtt gaaaggttac tgtaatttct ctttctttgt cctggatgtt tgaaatgtaa
     9481 acacctctac ggctaggaat agcaatagtt tcaatttctt ggctaacaat accacggcct
     9541 gacacatgta attgcctata ccctggtatt aattcattaa gcggtttccc gtttacggac
     9601 atattatcag atggcaagcg agctgttaca ctcgcaccac cttttaaatt tacataatca
     9661 tacattaata gacctctgtt aatcgtgctg tagctctctg tctatctgta atgtcatcta
     9721 caaaaagacc ataagctctg tttcctagtt ctaggttgag ttctaacggt tttgcctgat
     9781 gtccaagatc tacttcgaca cctgttgcca ccgctcctga tatagtacta ctcatgtcag
     9841 catcgaacag tgaattgcca tcaacaagtt tagatatgct atcagccatc ccagaaacat
     9901 tgtttttaac ttgaccgaaa ccgtttgata gaccgttatt aagtcctgac ataattgcct
     9961 taccagctgg aataagcagg cgtctatcgt aacttatagg tcctttgtgc gacttgatcc
    10021 aagaggcaat cccgcctaca aagctagtga tagcccccca catagatttt agaccgctta
    10081 aaaagctttg catgatggcg ctaccggctg aaaataggtt gatacttgat aaaaaaccaa
    10141 cgactgaatt ccacccgtta acaatgaagt ttttaactgc attaatacca ttacttactg
    10201 cgtttttcat ggcatccatt gcgccagaga caatcgcttt aatggcattc catacgcttt
    10261 cggcggtggc tttaacagca ttccatacag agtcccaaat agattttaaa gcgtttaaga
    10321 ttgtttcaat aactgatttc aaagcattga ataacgtttc tccaattgtt ttgatagcgt
    10381 tccaggctac ttcgaatatt gactttatcg cttcccaagc cgcttgccaa tctccttgga
    10441 taacagccgt gactgttcta attatctcag caacaacatt tattgctgta gaaacgattg
    10501 ttgtgataac tgtccaagct gactgaacaa gtatcttaat gacctcccac atggtttgga
    10561 taactgtttt tataatttca aagccagctg taataattgc tgctaaagta gtaagcgcca
    10621 cttgtacagt gttagatata ataacccaag ccagctgaat ataagtctta ataaactccc
    10681 acaccaatgt aatttgcaat tttataattt caaagcccgc ctgcactaca ggagtcaaaa
    10741 ttgctaaagc agattggaaa attgttacga taatattcca cgtgtcagta aagaattgtt
    10801 taatatcttc ccaaataggc tttatttgtt cgcctagagc tgtaaagaat tccgtcaaag
    10861 agctccataa atttttaaaa gcatcgacaa tagggatcat tcctgtaaca aaactttccc
    10921 aaacacctag agcagatgat tttatccctt cccacagagg tgagaagaaa tctgccaatc
    10981 cttgccaaac cgcttttaag aaatcaacga ctgaagccca tactgtttta acgttatccc
    11041 atatactcga agcgccgttt tttattgttt cccatagatt tgagaagaag cttggagcgg
    11101 cagcccatgc gtttttgata tactcccatg ctgtagaaaa tacttgtttt acagcttccc
    11161 agatgcctgt aacggcgttt cggaatcctt catttgttgt ccaaaggtat gttagtactc
    11221 ctactagagc cataattccc gctataatca gcgtgattgg tcctcctaaa gccgctataa
    11281 ctgccttaaa accggtcaat gctgttcctg ctgttttaaa tgctgtcacc aaccctttga
    11341 taaatccaaa tgctgttgct agcattctta ttcccgttat tattttattg ataactaaaa
    11401 atacaggacc gatggtagca aggaagatcc caaaacttat gataccgtct tggacttttg
    11461 ggtctaaatc actaaaagct tgagtgattt tctttattcc ttctgcaagt tttccaatcc
    11521 tcggagctag atgtttccct ataacggttc cagcttcaaa taaggcattt ttaagtaatt
    11581 ttagttggtt agcgaaactc tgcgaagcag catctgcttc tttttgcaga gcagtgccat
    11641 ccgcccacgc cttatttgct atgttaaacg atttggctag taaatctccc gcaccagaaa
    11701 gacgtgctaa agtgtcaatc tctcgcacgg aattaatacc taaatctttc aaagtttctg
    11761 tgacatctcc gccactctct ttgatttttc ctagtccttt cacaaagtca acaatagctt
    11821 cttgtgggtt ttttctccac gtttgggcaa attcttccgc tgttttacca gctactgagg
    11881 caaatttctg taaaccttcg cctccagaaa ggacatctgt attcattttc tgcatgactc
    11941 gagaaaatgc tgaaccccca gcttctgcat tgataccaac cgagctcatt ccagccgcca
    12001 aacctgctat ttgagcttct gtcaatccaa cctgatgacc agtaccagcc aaccgcaatg
    12061 ccatctctga gatttccgat tctgttgtag ccattttatt acctaaagct acaatggttg
    12121 accctaatct gtgaaactgt gtttgtggca tttgagtgat gtttgctaat cgtgccattg
    12181 cattagctgc ttgctcagat gacatatttg tagcagtccc catttgagcg gcgattctcg
    12241 aaaagttgat taggtgttta ccaccttcgt ccgtacggat accaagttgt cctgctacct
    12301 ctgctagatt ggcaatctcg acagcgctgt ttggcatctc ccgagacaaa gacataattt
    12361 gcttgctaaa tctatttaaa gcatctcctg atatgtccgt tgttttgcgg acaccaatta
    12421 atgcggtgtc ataatccgaa aacgctttaa gtgatgcgcc cccaagggca gtcagaggca
    12481 acgtcagacc tgctgtcagc gccttcccga cacccccaaa ggtgtcgccg atacgctgta
    12541 gcctgctccc cgttgttttt tgtaattcgt ttaacgcgtt ttttgcatct tggacacctg
    12601 ctctaaatcc gctggcgtct gctttaatta acgccgttaa cgtaaagtct gcaacaccgg
    12661 ccatttattc ctcctattca tttatgacac gatttaaatc agcgatagat aactttggtt
    12721 tttttggctt tgtatcaaag atagcgtcaa aagctttccc tgcatcataa aaatctttaa
    12781 aactcttata agccgaacgg acattttttc ctgtcccttt tgtcgcctta accgcttgat
    12841 taaaccaagc ttgcttagct aacatagtct cctcctcttg catttttact tgatgagcca
    12901 agtcataaat acaatagtca tgccatgtca gcctcttggc ttccaataag ctaagcccaa
    12961 accttgccat agctatcgcc attaaatcgt caaaggagta gtttatttgg ctttcgtcac
    13021 tgcttcgatt acttggtttc ctgcgatagc ttgctggagg actgcatcct tttttatttc
    13081 cgcaactact tcatcgtaaa acccaatgta agttccgttt acaatcgctt cttcgataaa
    13141 tttttcgagt tcttcattgg atggtttcgt tttctccgtg ctcgtagcgc attttaaaat
    13201 aaaaagcaag acttccggat ctttaaatgc gcttcctgcc tcaatttttg tcatcgaacc
    13261 tgcaccaatt cctacaccat ccatttcgat cgctctgtgt ttatttgcgt attttaaaaa
    13321 atcaaaccca aaaacgaggt catacgtttt cttaccaaca gtgatttcca tttactctcc
    13381 tttttacatt tcaaaactgt tttctttttt agtttcttcc ggttcagcac cacctctagc
    13441 catgcttttg tatctgtaca gcacatctga tactgcttgt ttttgctctg atgtcagttc
    13501 atctacgcct tctacaccgt ttccgttaat tgcgtaagtc atagatagtt ctacttgacc
    13561 atctgccggt gctgaaattt caaatgatgt aaagtatcct tggaaatatt ccgctttata
    13621 tttttgatga ccagaatcct tagaagtcaa atcaacttgc caaatctcca ccacatcgcc
    13681 atctttaaaa tattttcgca actcttccca aattgctgtc gtgctttcat cgctcgtata
    13741 agcaaaagac ttaatatcaa tggtgttttc tccatcagac aacgagttaa taattccgtc
    13801 ttttgttttt gttgcctcgg tctctttctc catcttaata gaatgttctg tttggaatcg
    13861 caattttgca gcatcttttg tctcaacatc atctaatcta cgaaagaata gcaaaaagct
    13921 ttttccttga attaattctg ccatttagtt tcctttcttt gtgtaagtta aatagacatc
    13981 taataccgta tgtaacaacg gtgtcgtgtc tgagttatcg ggtaaagtct gctcgttaat
    14041 acgatcaacc ctaaagttat aaccgtatgc agatgtcaga gagatgattt tgtcatgtaa
    14101 attagtaacg acattatcta tttcagagcg tttttttaac tctccataaa catgtattgt
    14161 ctgcctaatt acccccatca tttcgctgtt attgctaggc gtattagatg actccccaat
    14221 gtaagcaaat ggatatttag cattggcatt cggcaaataa tcataggtat ttttagttac
    14281 cgccaaacac tctttaaaaa tctgcctaaa aacggcgttg ttaggtgtca tttaaaggcc
    14341 ctcctgataa cgctgttata atccttttca agatctggta aaattgattc aatcgctggc
    14401 ctcaaaaatg gttgagccgg catttttctt gtaccatatt ccaaaaaacc cgagtatcct
    14461 gcgtttgaaa caatttctgc gcttgcttta gctggatagt gagtatatat ctcatcgtgc
    14521 aagaacccgg tgtcataagg agcaatcctc tgagcttctg ctttggcttt ttctgcatga
    14581 tttttaacaa ctgcatctgt taatctatca atgttgccag aggcgtttac cagcttgctg
    14641 aaagcttttt caataccctt ccactctatg gtcacgctag gcattacaac acctcctcca
    14701 gataaaaaac aatagcgcca agcttatttg cctgcgcttt gtatctcctg ccctcatact
    14761 cgcaataatc aaatgctttt gtataaggct gttttaggta aatgacttta cggtcctttt
    14821 gataatctcc aaaaatctgc acagacttct ccgcacccat ttccatcgca aaacaaggtt
    14881 ttgtctcgct tatagtctct gtgtgcgtgt actcacctaa atcagggtcg tattgctcat
    14941 ctgtcgtttt aacaaatgta actctatctg catatctcat aaaaagtata atcccccctt
    15001 acgagattta tccttgatta agtcaagttt tgccataatc atgttgtcgt atggttcaaa
    15061 ctcgtccaaa aagtcgtagt atgtcgtcgt atgaccttcc acactctcac tttttgccct
    15121 ttccgcacct ctacggttaa atctagcaat taaacaatct tctaaaacaa aactaaaagc
    15181 gctgtctatt ttgacaacgc catatttagc tttaaaatgg tctgtaacac gttttagcaa
    15241 catgttaagt aaatcatcct gttgactgtc taaaatgcct aaatcaagct ttacgttagc
    15301 tattatgctg tctgtgttta tctcttgcat aaacacctcc aaactatccg attgtttgct
    15361 ttaaaagctc caatagctcg gattttttaa gtttagcatc atactcaatg cttagctcgt
    15421 caagtttgct tttaatctca ctcactttca gcttgtcaaa gtctacagac ggttctaaaa
    15481 caccgccctc tgtcaaaaac tctactcggt cgcccgagta aatatcgccg acttcgtaga
    15541 ctacttttgt tactttatct ttaaatgctc taattactct aggcatattg acctcctaca
    15601 tttcaaagct gttttctttt ttagtttcgc cgttggcatt aataatttgt acttctgcca
    15661 agcgttcaaa tgatggcaat acaatcatag atacttttgt ttgtacgttt acagggtctg
    15721 ttgttttagt agttgtgaca gcaataccgt tttcaacgat cgacacctct gcatctgtgg
    15781 cttgaccacc catcaagtct gattgctctg gggttgtgcc aaataccgtg ctaccgagac
    15841 ttccgttagg gacaagtgta gcaacgccat ctgggaagta cttgcgtgct ttcccgtcat
    15901 caccgacaaa catgccgtct ttcaggatga ctttgacttg caactcatct tccagatatg
    15961 tgtttaagtc attcttggta accgctgaac cctgtggtgc cataggttta acaacctcta
    16021 cagtattatc tgcgttttta atcagactaa atgttttaga gttcatgatg atggcttctg
    16081 gtgcataacc acgctctgcc attgcttcga tagcggttgt aatatcggct agtggcgtag
    16141 cttgatttgc gtctgaccat ttgtttttag ctttagtcgt ctgctcttcg gtcattccgt
    16201 agtcaatatc tttcatgaca ccgtttgacg caatatggat tttaccagat gataaaactt
    16261 ccatgcgcat tgcttcaata cgggctttag cggcagagat aagcgttact ttgtcgttaa
    16321 agatagacgc caaaattgta tcaataagtt cttggttttt agtttgcgct aacatgttga
    16381 gttgttgacg gtcagcctct ttaacaagca tagcctcttt gaaaaatggc atttcttcgt
    16441 caatcaattc aactgacata cggtcacgta gcggcacttt agtgtcaaaa gcagctgctt
    16501 taagtgttac aggacgccct gctgcacctt tgataaatga taacttaagg cctagttgtt
    16561 gtttagacgg aaatgctttt tccccaagcg ttagctccgc ctcgcctttt tgtttgtcgt
    16621 aaaacccttt aatattttct gatgtgataa tctcatgaat gtaagccatt atttacctcc
    16681 ttcgataaat tggatgtgcg gtaatttttc tttcaaatct ttataatttt ccgcaataga
    16741 tggatcagca agcttatctg cattgatagt cccacgataa acacaggaac cgactgcatc
    16801 gccatttgtt aaatcaacat ctgtaagtaa gatgccgcag atgttgtttt cgctagaaat
    16861 ttctccattt tttacttcct taaccttttg tgtacggtct tcgaatacag atttttcttt
    16921 gccagctaat acagcaccag ctttgataat tttttcatct agttcgctag aatctagtgt
    16981 tactgaaatt gcttcgtaag gtagattgtg taaaatctcc ttagatgttg ttactttacg
    17041 tttattcatg ttttcctcct aaaaaagctt ggtgctttgt tgtgctgcct tactagccaa
    17101 gttagcaccg taattagttt gtttgctcaa tccaccccct gttgacggag tggtctggcg
    17161 taccagagcc ttgcggtcat cagcgataac tttagcaaat gcgtttgcta gcgttgttac
    17221 atttgctttt gtttgctctg catctaaagt cacaactaaa ccaagcacgt catcgttgac
    17281 gttgatttca gattccgcaa acatttgacg agctactgct gttaactcat tgcgtgtctt
    17341 atcgtttttt agctcttgca attcgtctaa cagcttctgc ttttcgtagt ctgctttctc
    17401 tttttcattc atcttagcca tttttttagc ttccgatttc tccgcttctt gttctgactt
    17461 ccactttgca aactttttat cgatgatggc atcaacatct gcatctgtgt actttttttc
    17521 gtcttgcggt tgttttgctt ctggtaccac ttgctcttca accgtttcaa ctgtttgtgt
    17581 ttcttctgcc atggttggca cctcctatgt tttaagtcgt ccccgactat aaaatccata
    17641 gctttttacg tcgtcaatgc ttggacataa taaaaagccg tattgctacg actttaaaaa
    17701 cttgttttta atttcaatca gcaggtcata ttctgatttt gtgatagtta ccatttcagg
    17761 gttattcacg aatagacatt ggtttctaac cctatctaat tcatcttgct ctaataccat
    17821 attatttctt cccccactta cgtttgtaat ttttcttaat atagttaacg tcaattgcaa
    17881 tgtttgtaat agctgcttga ttatcaaaac atacgccttt aaccgctgcc aattcgttat
    17941 ttgttgcttg gacgttttgt tgtataatcg ctctcagctc tgcaatttgt ctgttttgat
    18001 tcttaatagc ttcagcttgc atggcattct ctgcgataag caacacaata acctgttcga
    18061 gtttacgttt tttcttgatg cgtttattca ttaccttctc ctttatttaa ctttgttgca
    18121 cacgacattt cagggacaaa atttcccgca gcttctaagg cttttttgta agcttctttt
    18181 gtttcattga ctttattcat gatttgttga aattcgtctt tgttttccca agtcatttcg
    18241 atatttaatg atattttatt catgtttcct cctgatttta ttttataaca ctgccataat
    18301 cgacctctcc ataagttgag cacctacaat tcggatgtag cggtggcatg gttatagctg
    18361 ttgccccctt gctcaactca aatcgttctt tatcgtaagg tgtacaaatt tggcatgctc
    18421 caggctcaca aacaaatata taatgcgtaa acccattttt cttataagca tctaactgga
    18481 cgtcacttct aatccttgcg ctttctgtct taagtaatct tagcgcttcg tgttctgcga
    18541 catcaaagcg tctcatcaac cgttctttct cttttttata tccttgcata tctgtagcga
    18601 ttcgagaaag cgaactaaaa aggctttttt gtgtctcgtg atacaatccg ttatttcccc
    18661 agacacgctc ggaaaagttt tttccgtaaa aatctgcatt taggatacct tttaaccttt
    18721 tggcaacaga cccttgacct cccaaaattc ccgcttggcg tttcatttcg tccaaagcaa
    18781 ttttttctct tgccaactgt atctggttat caatcttagt gtgcattttg agtagctcat
    18841 aatttaactc ggcacgcaga agttcttctc tgcttacctt cattttcaga ttatacacgc
    18901 taagccacag attagcttct ttgctaaagt cacgattagc aaccgcctct ttcgccatat
    18961 tttgataagc tgtaacatca aaactgtcgg ctaacttctt cacagtacgt ttatcgagac
    19021 cgtgtttgtc agcatagcgc atgtaaaaac gatctatgtt agcctcgatt tgtgcctttg
    19081 actgcgtata taactgcttt aacatagctt cgttttctaa gtctgctttc acccatgcat
    19141 ccatcgcttt tctttcgcga ttataagctt catttcgttg tttctgtgtt gatttcacgt
    19201 cttaacaccc ccaaatcaaa gtctgatagc gacgggttgt tagattcctc ctctcgacgt
    19261 tctgactgga tgttttcaaa ttcttttcga gcatcttcca caatatctgt gatagccatg
    19321 atggtacctt gcgataattc cccacccaat gctttaaagg cttcaatctt ttcttgtagc
    19381 gacttaggta ggtttggcgt aaatgtgatt ttaagcttgc tgatgtcaaa gtcatcaatc
    19441 tctttaagca gttggcttac acgagcgatt agtttgtacc tacgtttaag cgattgctca
    19501 aataaagctt gcatatcaac acgctcttga tcaagaccaa acactttcca cttcagtgcc
    19561 tcaccggatt gttgaccggc aaattttgtg tcggtcatgt ctggcgtatt ggtaaattta
    19621 tggatgtcgg acacaatacg atttttatag gcttcggttc cttgtacgtc atattgttta
    19681 tatagatatt tggcatctac ggacccttca cgcccgtctt ggtcgacagg aggctctaag
    19741 ttaagtaatc tagctttacg catcttacgt aagtattcaa tggctttttc ggcagtgtcg
    19801 acatagccag gaaatgatac acgaccaatg attgccagaa ttgcatctga caaatcttgc
    19861 atgtagttag ctgtgtcaga ctgcgctgca tcatacaagt cgattaaaga caactctgtc
    19921 tcgtagtcgc ccatgccatc atctgtgttg agatactccg tgattggcac tgaaccaaac
    19981 gcatgagagg aagcctctcc aataggtgtt agatcaccat catattcaaa cttcaggatt
    20041 ttattatcgg tgtaaagttc gactattttt gttgtcccat ctaattgatt tttgttgtaa
    20101 tatcgcacac cagccaagct tgattgctct aggttatttt gatagataac aaacacttcc
    20161 cgtgggtcca gtctcacgac ctctgtcttg tcatccatac tacgataaat caattcaaac
    20221 gctcgaccaa ccttagatag gtcttttacc agtctgcggt taagttgatg gaaattattt
    20281 ttaacagata agtcctttaa taagtcttgt tgctgctcag taccatcgat atactctaca
    20341 cgaataggat tccccaccaa atagccctgt ttgagtgttg aaatatactt accatagtta
    20401 tgcacagcac gaacatcagc catgtcatcg tctttcctac ggcccatttc gctaatggtg
    20461 tggttatttc cttctgcata gtctaaaagc tcttgtatgc gtggtttttg gactttatcg
    20521 tggtgttcaa tcatttcacg gagtagctta tatttttcgg ttaacaagct ttctagactt
    20581 tccgcttgat acctcatgcg agcttcacgg tggaatctaa actctaatgt tttagtcttt
    20641 cccgtactat ctctaaatga ttctgtgtac attattttcc tttctaatat ttgagtcctt
    20701 ggcgtaaaat gttaaaggta tttgtgcttt gtttgcgtgc aatatgatac ttctcaagtg
    20761 catacctgat ggcatcaacg atatggttgt ttttatctat cggttcattt aaccaattac
    20821 cttccttgtc ctgtttaaag gtatacgtgt taaactcctc gatagtatgc tcacaagatg
    20881 gatgtatatg gattttaaat ccttggataa actgtattcc ttgcatgata gaccctttac
    20941 cttttaaact cggcacaata ccagatatgc ctttactcct tatctcagca atcaagcgtt
    21001 tttctgcgct atctgcggcg atgtaagatt tgtgcatgtt tttatctctt atcattttga
    21061 caatatcgtc tgttaacatt gctttttgat agtgctcgtt gtaaagccat aactctttgc
    21121 tttctaaatc cacagctaca catataagcg tagtagggtc ttgggtaaac ccaaagtcca
    21181 tcccgcttga cgtgtcatgt tttgatacta ttttgtcgat atcaaaatca atcacttcaa
    21241 agttatcaaa gacaagcccc tcagctactc cccattcgcc atcgcagacg attctagccc
    21301 gtctaggatt tgttttgtat aaatctaagt agcgtttcct atcgacctca tctagccact
    21361 catttactct aaaagtcgtt gtgtctgaaa aggtatcatc ccgctttgtc tcttcatcaa
    21421 aaaagacacg tttaagccaa tgtctttctg accacgggtt aaacgtgact gttatctgtt
    21481 taaaaaaatc aggaacatct aaagtaccac ggattgattc tacaactgtt gaaaacttat
    21541 cttctgtctc tatctggtaa gcctcttcaa accaagccca gcacaaagcg ccagcatcga
    21601 cagtaataga tgtgattttt aactcatcat caagtccacg gaataagatt ttttgtcctg
    21661 ttgccttgac ggttatctct ggcaagctct cattaaactt aaaaaggtgt gtaaccttta
    21721 attgattaca cgcccattta aaatcggtat aagtagattg tttgttagtg tttgagtatc
    21781 tacggataac caataagtta gcccaagggt acttcagcag tctgacgata aaatttaaag
    21841 cagtcgtttt agatttttta gatccacggc tgcctttaac tactcgataa aaatttttag
    21901 accgccaaaa agcgccataa cctataccga tagttttagg caaatcaacc ttgatttcta
    21961 gctttggttt agtctggtat gtcgtcttca ttaacaaaca ccaccgttcc agaatgctct
    22021 aattcacgtt tttctgagta agctccatta actttataaa ttctatccct agccctaccc
    22081 tgttcttcgt gacttggggt atattctctt ttcgtgtgga taaccacttg acctgtcagc
    22141 atatctgttt ctttgaattc agatatttgg atttctccac gggctatcga tgtatcttgt
    22201 tccaacgttt ctgcaacatc ttgaatacgg tctcctttag tatcttttgc ctccaaaaaa
    22261 cgttccttca ttgctttttg aatttcaaca tttttcaaca agcgttgacc ttggctataa
    22321 gcgctttttt cagaatatcc agcttgtttt gcagactcag tagcgttaaa tgtggtgatg
    22381 tacttatcca caaaacgctg ttgtttttcg gttaattcct cgtatgcgat tttccaccac
    22441 ctccaatcca taataaaaag tcaccacaat gtgatgacta attggttaac tatagataaa
    22501 tagcgaatga atgctaagcc tattgcctac cccattctgg gacacttcta tttatcgaac
    22561 aggaacagtc ggaatcgaac cgacacatct tcttctggct cttcgaaaag agttttcgga
    22621 cttagctatt gtcccgaagc aaggcgctac ccttaccgtt ttccaatcac ggttcatgtt
    22681 ccaaaggttt agaagagcta agacctctca caaacgctct tctacagacc ttgcacgaat
    22741 cgaacgtgcc actgcactca atatttagtg agtatttcct aatgcgaacg caagtccaaa
    22801 taaacagtct aaatattatt aacggtgccg tttcgccaca aaacttaagc ccacgatagc
    22861 ttatcacacc gtgcacagtg caacaactca cttcggccga atcctcaacc gaactatcag
    22921 ctaaccacaa gcaaggttgc gacccttgtt ttacttgcgg ttaatcaatc gacttagcgc
    22981 tggtacgcat gttaagaggc gttgctaagt cttaatcatt aacaccgaac ccagattgcg
    23041 tattctcctc aaaggaacgc caatgtcaaa taagggtgtt aattcacggt ggacatcgca
    23101 aagttccgtg attctttttg ctttgattac attccgcaat gtcacagcgt gtaatctctg
    23161 ggtgattgag acggcaggat tcgaacctgc acgtctcaca taccataaaa taacaagttt
    23221 gatcgtagtt aaagttggcg actaaatgaa tagccaattg gttaaaaggt tatctcttct
    23281 tgctattttg ctatactaca atattaacac agctattagt attatgtagt atcaatttgt
    23341 atctaattag tattttttag tatcaattcc agactttcct ttcctcgtct aacaaacata
    23401 aagtatttat tacgatttcc tatgtttaag cgttctctgg ctctctcata gtctccatca
    23461 cagtcaagat aagtcgtgag taaaacgtga ctctcacaaa tgcccatgct ttgcacaatc
    23521 aaattagcca tttcttgtcg acggtctttt aaacgctcaa tctgatcact ataatagcta
    23581 accatatgca gcatctttat gtttttgtct tcctgtgatt gtttaacccc accagacact
    23641 cgcatatcag accattgagg agacttaacg agcgaccgac tcattaggtt agcgtctctt
    23701 tcaagggtct ctatgagatg tgggatagtc tttaattctt ccaaaaaatt attagctttt
    23761 gttgtcggaa tgttgcccat ctaactctcc attcatgata taatattgtt gggtatttaa
    23821 tcatgaaggc gttcgcatgg acgtcttttt ttgtggagaa aagtcctctc tttctttttt
    23881 tgtttttcga cacaggcgta cgatgtcagt attagcgcct tgaataataa caaatgaccg
    23941 ataatctgcg ttagatttgt tttggtgtaa ggaggtcctc gtttctattt tttaatttcg
    24001 gtcaatacca accgcacgag tcgaacgtgc gtgataccgt tattggttat atccattcaa
    24061 ttagtggatt ttcaatgtgt tctatcccat caccaatcca ctctttgaca ttaaattctc
    24121 gctcaatatc ttgagtcctt ggcataacgt taatatcact aaaactcagc atgtcgtctt
    24181 ttgtattttg caaaaaataa atgtttttaa cttgtcttgt taaagagtcg ccatgcacca
    24241 cctctgaatt gattcacttt aaaaaattct aaatcactag taattgtata ttgtaatttc
    24301 acgacgtctc cctcaaaaat ctcaacaccg tttttatcaa ataggcctgt ggattgcatg
    24361 agaatataat caccaaggtt atcctcgaca aaatgaaacg tctctaagcg accagagcga
    24421 aactcatcat ctgctaagct gcatctgtat attttgcgtt cacttgattt aaagccatca
    24481 atgctataca ttttttggat ctttttatta aatgctctaa attttggtat cattattact
    24541 cctcattcca tatagtcatc ataaatccaa atttggtctt tactaagtat taaatcgtgt
    24601 cctgttttat ctattaatat ttgtaattgt tcgagattat caatttttat aaaaatatca
    24661 ggaaaataac caatttttgt aatttgataa tgtatgccgc atgattttaa atcttcaaca
    24721 tattgttcat ctttaaagct ggctgcatgc aactggtaaa tacaaccatt aaattttgat
    24781 ttactgtacg gataacgatt tggtttcttc attccgcttc cctcataaaa tattctgtcg
    24841 ctcgcttatc atttgctaac tctagttttc tgataaactg catcgcttct atttttgtta
    24901 caaactcatg ctcctgaaac aggtttttgt catagatcgc gtaagtcgct gtaatgcctt
    24961 tgttgtaaac tctcacaacg tgttttacca tttttaacta ctttcttttg ttttactttc
    25021 aagccactcc caaataagat gaaattgacc attaattaat gagtcgttac cataagtttc
    25081 gcaaatcgta acaattgact ggttggccca tatccaataa gcttcagtat taaatccaac
    25141 ttcttgcatt ttgacattac tctctctcat ccagtcagga accactcgtt caaaaaaatt
    25201 aatataatca attttcataa ctcttccacc tttacgtata ttcctactgt atcagcccag
    25261 aacttctcaa taatttccga agcaacctgc gcatcatcat tccaaaatcc caatgcagtc
    25321 atacaatctt taggtaactt gttcaaatta tctgtgtcgg gtttagtatc tttatattgt
    25381 ccgtctactg attttttgat tttcgggaat aaccatttga cagtgagtcg taatggaccg
    25441 gtcatttttt tctcaggagc atggggtgca aatttactca taaacatggc acgagcttcc
    25501 ttcagcgaat ccggttcgta gaattgtggt ttaccgttta ccacacggac cttcttttgt
    25561 tggtgtgtag tagttgggat tttcttcatc gggataaaga aatcaatcat caattaagcc
    25621 ctcatattta acaaatacga tgatctcatc ttcatcaaaa atgaattgga tatctaaaca
    25681 tttaatacca tcttctcgca ggtcgtttat aaaaccgtta acttcatttt ttaaatattc
    25741 caactcaata tcacttttac catttttaaa aaattcaatt tgcatttttt tattcctttc
    25801 ttaatctacg cgctttgtca aagatgagaa aggaatggtt ttggcgaagt ctaacgccaa
    25861 accttccttt ttctttgact tttgacgaag gaaaaacact tttaataact ctgataagag
    25921 ttatagcgtg tctttttccg gaaaataaca taaaattctc tgttttttac aggatttaga
    25981 aaaagggaaa atttatgttt tttacaggat ttaggaaggg aaaataacat aaaattctct
    26041 gttttttaca gggaaaattt tctgttattt tctttcccta ttctgttttt ttctgataga
    26101 caattccgtt tttaatatca aaaccaccat gctctttgag atgatttcga actgtttttt
    26161 cggtagaatc cagatactct tgcaactctg ataatgaaac gggtgtgaag ccatcaaata
    26221 gagcactgta ggctgtatct attttttgct tacgttcctc tgccttttct ttttttgact
    26281 ttctaccttc cttagctttc tgccagttcg gcttactttc ctccagttgt atatctgcca
    26341 acacaccagt tgtatcaact tcgtgcactg gataactaaa ccacatgttg accggctgaa
    26401 atttagcaaa ttcacgtaaa gttccttcaa ctcgccagcc tgtgctaatg ttgatagcgt
    26461 ggacagcctg cttgatttcg tctgtgtaaa ttttacgtac taggacatca ctaatcgctt
    26521 tttcaaagtg ttgtctcatc tgtgcattac tttccaaatc gtcaaggcta acatattgtt
    26581 ggtagtagcc tagatttcgc tcttgcaaag cacgttgata gattttattg gcagccttat
    26641 cagcacgaac cttaatcaaa tcatcgctaa gctcaagctc tactaaatca atcaatgcat
    26701 caggatcacg agcaaatact ccagaaccac ttgccctgtc catggacttt ttaccacctt
    26761 gacttccttt agaatggtgg tggcagtaaa ttacgctaca tccaagttcc gtggctacct
    26821 tatcaaattg atttgtaaaa tgggccatct gatcagcact gttttcatca ccagtgagaa
    26881 ctttataaat agggtcgata ataaccgctt ggtaattttt cttaagtgag cgtctgataa
    26941 gtttaggtgc taacttatcc attggtactg ttttcccacg gaggttccaa atgtcaatat
    27001 ttgccacatt agtaggaggt aatcccattg catcataaac atctttaaag cggtgcaaag
    27061 ctgatggtct atcgagctcc aaattgacat ataaaacttt tccttgttca cattgccagc
    27121 ctaaccattt ctgtccctcg gctaaagcga tagataactc aatcaaagca aatgacttgc
    27181 cagctttcga tggtcctgca attagcattt tatgtccctg gcgtaacact cctttaatta
    27241 attcaggagc aagctctggc aaattatccc attcatcagc tagcgtttca ggctctggta
    27301 aatcatcatt taaatcttca atccattgat accattcttc ataattggtt ttaccaatat
    27361 ttgtatcaat caaaaattgc ttgtgaccgt ttcgcataat acctggcatt cgtgatagac
    27421 gacttggatt acgattctgt gtgtcaatat caagaccgtt tttcttacag atttgataga
    27481 tgtaatcaac ccgtttgcgg tactcctgat aatctctagc atctactttg acaatagcgt
    27541 gtaatgattt tttaccgcta tggaccaatg tcgctattgg caattctagt tctttaaata
    27601 aagcatactg tttaccaagt tccatgctat cggattcaac caaggcatac ctaaagtctg
    27661 ttacattgtc gttcttgacg ccattgccat ctaaagggtt aaatcgtatc catgccccag
    27721 cttcttcttt gtagtcgcca agcacagcac cgatgtcgtt tccgtatttt tgcaactctt
    27781 ggataagttc gcctgctgta cgtgcgtagt ccccttttgt tggtttgtag actacccctt
    27841 catctgt
//
LOCUS       DATURV010000017        27835 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DATURV010000017 DATURV010000000
VERSION     DATURV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27835
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.017"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..1278
                     /gene="rocA"
                     /locus_tag="VCF75_000730"
     CDS             <1..1278
                     /gene="rocA"
                     /locus_tag="VCF75_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEP2546311.1"
                     /translation="SFVSAIPLRLKNIFYLSLSMVLFQVVFWAFFPDHFILDVVMLAQ
                     FLFFALIALYYGKSIKAKFLMFYAFFPLVSISLVKRFIVFFVMPLFGMPYSVVKHNTL
                     LIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQSHRASKLLVFTNSSMALYYLCVQGID
                     VMSPSLSGLATTTARSIIVLFYFILFLTLLIHLERYVKQNSIEAIVQQKEYRELINYS
                     QHLGLLYQDIQELRQLLTTVSSRLKIGIEQNDISIVRLTYEGILNAEKNNAKDDRLDL
                     TCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVSIPNCIATFFLEVVDFTKLLSFLLDNA
                     IEMSLETKQPCLSIAFLDQNHKLVIVIQSSTKQGQDDSQSVFAIPALKKRDDWQFDLR
                     NVTTILNRYDYLTISSQIHDGILTQLIEIAKPD"
     gene            complement(1379..4084)
                     /locus_tag="VCF75_000731"
     CDS             complement(1379..4084)
                     /locus_tag="VCF75_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEP2546312.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTNP
                     NFHSFHLDGQTIILDDYLKVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDKWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDDSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLADLADDLPENL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLMQNHPHDSICGCSVDSVHREMMTRFEKAYEVGHYLAKEAAKQIADAI
                     DTRDFPMDSQPFVLFNTSGHSKTSVAELSLTWKTYHFGQRFPKEVYQEAQEYLARLSQ
                     SFQIIDTSGQVRPEAEILGTSIAFDYDLPKRSFREPYFAIKVRLRLPITLPAMSWKTL
                     ALKLGNETTPSETVSLYDDSNQCLENGFLKVMIQTDGRLTITDKQSGLIYQDLLRFED
                     CGDIGNEYISRQPNHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPVSADKLLQA
                     EMEAIIDITERQSGRSQEKAELTLTTLIRMEKNNPRLQFTTSFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPNAAFWKNPSNPQHQECFVSLFDGEKGVTIGNYGLNE
                     YEILPDSNTIAITLLRSVGEMGDWGYFPTPEAQCLGQHSLSYSFESIDRQTQFASYWR
                     AQEGQVPILVEQTSHHTGSLRAEHSYVTGADDQIALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(4099..5385)
                     /locus_tag="VCF75_000732"
     CDS             complement(4099..5385)
                     /locus_tag="VCF75_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEP2546313.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTIFSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSIAP"
     gene            5518..6558
                     /locus_tag="VCF75_000733"
     CDS             5518..6558
                     /locus_tag="VCF75_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2546314.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAKKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            6609..8303
                     /locus_tag="VCF75_000734"
     CDS             6609..8303
                     /locus_tag="VCF75_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEP2546315.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDMPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAELTVKHFFNRQLDKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFAMRGIIEGFYGTPWTREERLDCLRFIGRKRM
                     NTYMYAPKDDDYQRKQWRDLYPEDWVTYFKGLLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAIYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQSDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSHSWLETLTDYTEDTELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKNVSTLSAWVDELVELVNTLRKLDKPAFQQAIAPWFERVAKDQDFWQAM
                     LNQEPQLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            8328..9770
                     /locus_tag="VCF75_000735"
     CDS             8328..9770
                     /locus_tag="VCF75_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP2546316.1"
                     /translation="MLAFPKDFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPTLFYD
                     YVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQVYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGR
                     IGTILNLTPAYPASQSEADTAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDAYLMPGRRMN
                     VDKGWEIYPEAVYDIAVKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIRF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(10381..11259)
                     /locus_tag="VCF75_000736"
     CDS             complement(10381..11259)
                     /locus_tag="VCF75_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP2546317.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAVSAPGAVNKEKGIIEGASAIPYIHHFKIKEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVIGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALTEQGDPLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            11455..12405
                     /locus_tag="VCF75_000737"
     CDS             11455..12405
                     /locus_tag="VCF75_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2546318.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLTGSG
                     ADPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            12415..13341
                     /locus_tag="VCF75_000738"
     CDS             12415..13341
                     /locus_tag="VCF75_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP2546319.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            13422..14867
                     /locus_tag="VCF75_000739"
     CDS             13422..14867
                     /locus_tag="VCF75_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP2546320.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIDDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            15173..15778
                     /locus_tag="VCF75_000740"
     CDS             15173..15778
                     /locus_tag="VCF75_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEP2546321.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLVTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            15768..17492
                     /locus_tag="VCF75_000741"
     CDS             15768..17492
                     /locus_tag="VCF75_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2546322.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGFSS
                     YSISSTYKRVDAEAQMRLEETLGRLQSQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADKVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQDHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            17492..18976
                     /locus_tag="VCF75_000742"
     CDS             17492..18976
                     /locus_tag="VCF75_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2546323.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDWLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAAALTGFTVLYLDCQEWQAAIDAYFEREGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            18906..22412
                     /locus_tag="VCF75_000743"
     CDS             18906..22412
                     /locus_tag="VCF75_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEP2546324.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNVKQEIDMTPDSKEQTVTDYQ
                     YHYIDQEGRKQPFNQGWRFLMADVTCAQDPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENANYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYANGKDYDRLHEEHSDWLLYGSET
                     VSAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNLTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPARLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKASQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKASTPHILITSYQHY
                     LPLPLQVCSQAFDFKKGDDIFLPQWFQVKDAQGSFVKKAFPMTWYLKEKIFAEPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLAASQKRLTYCYQYDTVQAIGA
                     ISLQNHTGTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLAVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            22550..23428
                     /locus_tag="VCF75_000744"
     CDS             22550..23428
                     /locus_tag="VCF75_000744"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP2546325.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEVRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            23663..24418
                     /locus_tag="VCF75_000745"
     CDS             23663..24418
                     /locus_tag="VCF75_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEP2546326.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVAVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAQAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            24471..24809
                     /locus_tag="VCF75_000746"
     CDS             24471..24809
                     /locus_tag="VCF75_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP2546327.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAGIPHALYAVEAFQ
                     MLLVVVKPEV"
     gene            24811..25116
                     /locus_tag="VCF75_000747"
     CDS             24811..25116
                     /locus_tag="VCF75_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP2546328.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPKDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKVHHLEAKTDCKLLQVLLD"
     gene            complement(25552..26625)
                     /locus_tag="VCF75_000748"
     CDS             complement(25552..26625)
                     /locus_tag="VCF75_000748"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEP2546329.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQTKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKSLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKHIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(26681..27433)
                     /locus_tag="VCF75_000749"
     CDS             complement(26681..27433)
                     /locus_tag="VCF75_000749"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEP2546330.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(27673..27743)
                     /locus_tag="VCF75_000750"
     tRNA            complement(27673..27743)
                     /locus_tag="VCF75_000750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27709..27711),aa:Thr,seq:ggt)
BASE COUNT         8060 a         5351 c         6063 g         8361 t
ORIGIN      
        1 agctttgtaa gtgcaattcc gcttcgcctg aaaaatattt tttatttatc acttagtatg
       61 gttttgtttc aagtagtttt ttgggctttc tttccagacc atttcattct tgatgttgtg
      121 atgttggctc agtttctttt ctttgcgctt atagccctat actatggtaa atcaataaaa
      181 gctaagtttt taatgtttta tgcctttttt ccactagtgt ctattagttt ggttaagcga
      241 tttattgtat tttttgttat gccattgttt ggtatgcctt attcagttgt taaacataat
      301 acactactga tttacagcat tacttgtttt agtatttttt tgatttatcg ctgcattcaa
      361 gtttttcatt tcgatttttc aacctggcgc caatattttc aatcacatag agctagtaaa
      421 ctattagtgt ttactaactc ttcgatggct ctatattact tgtgtgtcca agggatagat
      481 gtgatgtcgc cttctttatc aggacttgct acaacgactg ctcgttcaat catagtgctc
      541 ttttatttca ttttgtttct tactttatta attcatttag agcgttatgt aaaacaaaac
      601 tctattgagg caattgtgca acaaaaagaa tatcgtgagc tgattaatta tagtcagcac
      661 cttggattgc tgtatcaaga tattcaagag cttaggcagc tattaactac cgtgtccagt
      721 cgtcttaaga ttggaatcga acaaaatgat atctctattg tgagacttac ttatgaaggt
      781 atcttgaatg ctgaaaagaa taatgctaaa gatgacagac ttgatttaac ttgtttagat
      841 aaattacaag ttgaagcaat cagacatatt gttttagcta aattaattga ggcaaaaaat
      901 aagaagctta aggttgaggt atcgatccct aattgtattg caacgttttt tctagaagta
      961 gtagatttca ctaagctatt gtcatttttg ttagataatg ctatagaaat gagtttggag
     1021 acaaagcaac cttgcttatc aatagcattt ctggatcaaa accataaact tgtcatagtc
     1081 attcaaagca gtactaaaca aggacaagat gatagtcaaa gcgtgtttgc tataccggct
     1141 ttgaaaaaaa gagatgactg gcaatttgac ttaaggaatg tcacgaccat tttaaatcgt
     1201 tatgactatc tcacaattag ctcgcagatt catgatggca ttttaaccca gttaatagaa
     1261 atagctaagc ctgactgatg ttaaaggtat taataatcac tcaaaatcaa cttaagagtc
     1321 tgagacaata gtcccagact ctttttgaat atgttaatct attactaatg ataatagttt
     1381 attgtttctt ccaagctaga gttaaaattt ccgctttgcc aagttggctg ggtgtgcttt
     1441 gcttgctgtc tttttctaac aaattagtga ccttggcatt gtagtttggc aggcttaggc
     1501 taaagtcaca agttttatcg tttgagagat tatagctgcg cgtgataagg gcattgtctg
     1561 ctaagcgacg tttgaaagct gttagagcga tttgatcgtc tgcaccagtg acatagctat
     1621 gctctgctct taagcttccc gtatgatggc tggtttgttc gactaggata ggaacttggc
     1681 cttcttgtgc ccgccaatag ctggcaaatt gtgtttgcct atcgatgctt tcaaaactat
     1741 aagataggct atgctggcca agacactggg cttcaggtgt tgggaagtaa ccccagtccc
     1801 ccatctcacc aacagaacgt aagagagtga tggcaatggt gttgctatct ggtaaaatct
     1861 catattcgtt gaggccgtag ttaccaatag tgaccccctt ttcaccatca aagagactca
     1921 caaagcattc ttggtgctgt gggttacttg gattcttcca aaaggcagca tttggatgat
     1981 ttggacgttt gacagtttca aaaatactat cagctaggtg atggtctgtc ttaaggtgcg
     2041 ttgggaatag gacgcgcaag cgatgattag tcatttggtt atcaaaactt gtggtgaatt
     2101 ggaggcgagg attgtttttc tccatgcgga taagggttgt taaggttagc tcagcctttt
     2161 cttgtgaacg tcctgattgg cgttctgtga tgtcaatgat agcctccatt tcagcctgta
     2221 agagcttatc tgcggagaca ggaatggcaa aggtttgctg gatttcaagt tcagcaactt
     2281 gagcggtgtt gctaatgatg ttaagcttgg tggtcccttg gtccgcatag aaaggttggt
     2341 catgatttgg ttggcgagaa atatattcat ttccaatatc gccacaatct tcaaaccgca
     2401 acaggtcttg atagattagt ccagattgtt tatcggtgat ggttagacga ccatcggttt
     2461 gtatcataac ttttagaaac ccattttcaa ggcactgatt actgtcatcg tagagggaaa
     2521 cggtttctga aggagttgtt tcatttccta gctttaatgc taaggttttc caagacatgg
     2581 ctgggagagt tattggtagc cgtaatctca ctttgatggc gaaataaggt tcgcggaagg
     2641 atctcttggg caaatcgtag tcaaaagcga tgcttgtgcc taaaatttct gcttcgggtc
     2701 tcacttgtcc actagtgtca ataatttgga aagattggga gagtcttgcc aaatattctt
     2761 gagcttcttg gtaaacctct ttaggaaagc gttgaccaaa atgatatgtt ttccaggtca
     2821 ggctgagctc agcaacactt gttttggaat ggccgctggt attaaataag acgaagggtt
     2881 ggctatccat tggaaaatcc ctggtatcaa tggcgtcagc aatttgctta gcagcttctt
     2941 ttgctaaata gtgtccgact tcataggctt tttcaaagcg cgtcatcatt tcccgatgaa
     3001 cgctatcaac actacaacca cagatagaat catgagggtg attttgcatg agggttttcc
     3061 aagcgtaacg cagttggtcg tgagggtagg tacttgttac ctcataagcc attgctgcta
     3121 agggttcggt gatgttttcg agttggcgag agactctggt attagcctgt ttgaggtaaa
     3181 tacgagcaga agccgtgtta gctagggtat accagccatc ggtttcttga ctggtaatct
     3241 ctccttggac ggttgaaagg ttctctggta aatcatctgc gagatcagcc aagtaatctt
     3301 caaagcagga atgcacaaat tcgtagtcag gatagagttg gttggctaag gcgattgcct
     3361 tggtgacatc aagttgtacg ggttgatgat cacacccgtt catcatcaga aggtgcttgg
     3421 ttgaggcgaa gcgttcggcg tcagcaagtt ttttatccca aaaaagacgc gcctcagctt
     3481 ctgttgtcgg gatctcattg ccattgctgt accagttggc gaagaggagt ccaagaatac
     3541 gactgtcatc tggtccttgc caactgattt cagagaattg ggagctgtat ttttcactgg
     3601 tatctacttg attgttaaat ccagttggac gaatgccacg accaaaggca gcagcttgta
     3661 ggccggcttt taacatcagc tgtggtgttt gtcccatatt tccaaaggtg tctggaaaat
     3721 aaccaagtgg cacactagcg ccccatttgt cacaatcctc cttgccaatg agcatattgc
     3781 gcacattgga ctcgctgctg gtcaaaaaat cgtcctgtaa gatatagaaa ggtccgatac
     3841 ggagttttcc cgaagcaatg gcttgtctaa tctcaggttc tcgttcgggg cgtactttta
     3901 aataatcatc taggataatg gtttgaccat ccaaatgaaa actatgaaaa ttaggatttg
     3961 tttgaaaaac ttctaacagg tcatctatta agttaatcag acgcatgtgg tgttgctcgt
     4021 aagccatgta ccactcgcga tcccagtgac tgtgtgaaat aatatgtact tttttggttg
     4081 ccatagtggc ctcactttct aaggagcaat gctaaagcca agatagtcaa gaagtagttc
     4141 acagaacatc atgttagccc aagagaacca ctcacgggtg tattgataag gatcattgac
     4201 atcgaaaccc tcgtgcatta agtgggtacc agcatctgtt gccacaagca aatcaagcat
     4261 ctctttttta ctgtcttggt caagagcggt caatccttgt aaggcaaggg cgatatgcca
     4321 gatataattt tctggtgtat gggaagaacc gatgcctgct gctgcatttc cctgataata
     4381 ataagggttt tcttggctga aaatggtgcg gcgcgttgcc agatagatag ggtcatcttt
     4441 agcgcaaaaa ccaaggtaag gtgcagcaag gaggcttgga atattagcat catccatgat
     4501 ggaaaactgt cccagaccat ccacttcata agcgtaaatg gtttctccct tgctgttttg
     4561 aacaagggca tggtctgcaa tacccgattg aatagcttct ttcagctgct tggcacgagt
     4621 agctactggt tcattgtgaa aaaggttgtt atataaatct tctagatagg aaagcacaac
     4681 cactgcaaac atatttgaag gaatgaggta accatattga caagcatcat cacttggtct
     4741 aaagcctgac caggtcatcc ctgttggtgc gacaggactt ccttttccag catgtgttag
     4801 ggtatcttct ttgcgccaag tatcgcgttc aaacaagtaa ggagactggg cgtggtcttg
     4861 ctcaactgtc cataggtcaa gaatgagttc taccccacga tgaaaatcgt cattaaactg
     4921 atcggtgctt cctgtttcac gatagagcaa gtaagctagc tgaatagggt agcagaggga
     4981 atcaatctca tatttgcgtt cccaaatcca agggttcatt tgggtacgat ctgtttggtg
     5041 tccttttccg ttagcttctt cgttgaaggc attggcatag ggatcgatac aaatgtagcg
     5101 gaattggcgt ttgaccaaac cggcaataat ctttctaatt tcttcatctt ccttggcgag
     5161 gaagaggtaa ggtttcattt gcgcagtgga atcacgtaac cacatggcag gaatatctcc
     5221 tgtcaagaga aagctagtgc catcttcgta ggtttttagg gttgtctcta aggtattggt
     5281 aaagctggtt ttgaaattaa gagcccaatc agggtgttgg tctccacaaa ggctttccac
     5341 cttggccata aattggctga cgctagttgg gattggtcgt gtcatatcat atcctccaaa
     5401 aagatatatc atttatcttc ggtttcatta taaatgataa gttaagcttt gtctataagt
     5461 ttttgaaaag gtttacaact cttttttgac atgctatact gataacggga gtaaagaatg
     5521 acaaaattat tatacaaaaa gatatatcat ttcattaaag agcagatagc atcaggtcgt
     5581 ttgcaaattg gtgaccgcct acctactgaa aaagaattgt cagaacagtt ctcggttagc
     5641 cgtattactt ctaagcgagc gcttgtagaa ctggagcaag aaggcttgat tacgagaagt
     5701 cgtgggaaag gaagcttcgt agctaaaaag caagtgacgt cccctgaagc gaataaggat
     5761 ttattgttga ttcttccttt tgcttctgat tatgaattgg gagattatgc taagggaatt
     5821 atgacctcca ttgctgaaac aggttaccgt ctcatggttc aattggccag tacagtaagg
     5881 ttagacaccc tgtcagacta tgctgggatt atttattatc ctgaagatgt caatcacagt
     5941 attgatttcc tgttttactg cgaccatcat cacattcctc tagtcttgtt ggataaatct
     6001 ctagatcttt ttcagtttcc gtctgttgta gcggataata aaggcggtgc ctgtcaattg
     6061 actcagcact tgattgagca aggctgtgac cagattagtt ttgtggcaac aggctctttt
     6121 ggagaagtat catccgttag agaccgttat ttgggttact tagcggcaat ggcagaatcg
     6181 tctctaaaat cccattattt tcctaagaca gagactgaaa caacagatgc ttatttggac
     6241 cgtctggtga aaaccttatc ggagacgaca gccactaaaa gggggcttgt cgtggaaaac
     6301 gattggttgg ctattcagct gattcaaaaa atcacacagg taggcttatc tattcctgat
     6361 caagtcgcta ttgtaggctt tgataatagt caagcctccc gtttgttgag tcccaaatta
     6421 agcacagcag ctcaagattt ttaccaaatg gggcaagaag cagctaaagt cttgttgcag
     6481 attattcatg agccagacca agcagtggtg tcttgccagt tacctgttca agtatttatc
     6541 agagaaagta gtcactaaaa aagacgttac tagcatgagc tggttagctc aggtaaagga
     6601 gaccagaaat gaccatttat cacgccttaa aggattatca agaagtcatg acaagaggag
     6661 actatcttgt ctttgacatg ccgttgacat gccgctttat tggccgtttt ttccgttttg
     6721 aaaatcaaaa agccctactg gcagaattgg caacgtcaaa gtattttcag tggattgaag
     6781 aaggccaagc agagttgacc gttaaacatt tctttaaccg tcagctggat aaggatgcct
     6841 ttaccctgaa ggtttcagaa gataaggaaa ttattattga aagccaaaac cttagaggtt
     6901 ttcgttacgc ccaagaagct ttgcttaaag tgatgacttt caaaggggat aaactttatt
     6961 taccgattgt atctgtcaag catagtcctt cttttgctat gcgggggata attgaaggct
     7021 tttacggaac accgtggaca cgagaagagc ggttagattg cctgcgtttc attggacgta
     7081 aaaggatgaa tacttacatg tatgctccga aagatgatga ttaccagcga aaacaatggc
     7141 gcgatcttta tcctgaggat tgggtgactt atttcaaagg gctacttgca gtagccaagg
     7201 aagaagggat tgatttttgg tatatgatta gtccgggact tgattttgat tataccaagg
     7261 aagctgacta tcagttgctc tatcaaaaat tgcaacaact tctagcgtta ggtgtttgtc
     7321 attttgggct tttgctggat gatattgact accaaattgt agatgctgtt gagagacgat
     7381 tcaaaaagac tgcctatgcc caggcccact tggcaactga ggttcatcac tttttaaacc
     7441 aacaacatgc ggcgccagaa ttggtgatct gtccgacaga atacgacaac caccatgatt
     7501 ctatctattt gcaagagctg tcagaaagga ttcctaaaga agtcgccttt ttctggacag
     7561 gcccaagcac gctagcgagt caaatctcgc aagcagatat tgaaactatg gcagcgattt
     7621 accaacgtcc tatcatcatt tgggataata ttcctgttaa tgattatcaa aaagatccag
     7681 aacgtctctt tttaacccct tttgctaatc gctcaccatt tttatgccaa tcagactatc
     7741 aggtcaaggg gattgtttct aaccctatga ttagttggga attatctaag ctgaccttga
     7801 cagacatgag ccattatctg tgggatgcta accgttatca gcctagtcat tcttggctag
     7861 aaaccttaac agactataca gaagatactg aattagccct agctttacag gcatttgctt
     7921 ggcataatgg caatcgtcat ctgcacaggg accttccttt tgaagtggaa gaagctctcc
     7981 tggctaagaa tgtatcaacg ttatcagctt gggtagatga attggtagaa cttgtcaata
     8041 ccttgagaaa gctagataag ccagcgtttc aacaagctat tgctccttgg tttgaacgtg
     8101 tggccaaaga ccaagatttt tggcaggcta tgttaaatca ggagcctcag ttggaaacac
     8161 tctatgctga cttacaagaa gataaacacc gcattggaag tgacattcca agtcgttact
     8221 atcgcatcta ctatcaacag caagataaac taacagctaa tcaaggtcaa gttacccaag
     8281 caagacctga agattatgct tagtcatacg agctagaaag gaatgatatg ttagcatttc
     8341 caaaagattt ttggtgggga ggagccactt caggccctca aagtgagggg cgttttgcta
     8401 agcagcaccg caatctcttt gattattggt atgaagagga gcctaccttg ttttacgatt
     8461 acgtgggacc tgatacggct tcggatgcct atcatcagat tgaatctgat ttgaccttgt
     8521 tagcttcact aggtcacaat tcctaccgca cctccattca gtggacacgt ttgattgatg
     8581 attttgagca agcgacgatt aatcctgatg gccttgctta ttataatcga gtgattgatg
     8641 cttgcttggc caatggtatt cgtcctgtta ttaatttgca tcattttgac ctgccaattg
     8701 ccctctatca agtctatggt ggctgggaat caaagcatgt cgtggactta tttgtcgcct
     8761 ttagcaaggt ttgttttgag cagtttggtg atcgggttaa ggattggttt gtccacaatg
     8821 aacctatggt cgttgtggaa ggctcttatc taatgcaatt tcattaccct gctatcgtgg
     8881 atgggaaaaa agcagtccag gtcgcctata atctagccct agccacagcc aaggttattc
     8941 aagcttacag gcgaggtcca gcagaactta gtgatgggcg tattggaacc attctcaatc
     9001 tgacgccagc ctacccagct agtcaatcag aagcggatac ggctgctgcg cattttgcag
     9061 aactttggaa caatgacttg tttatggaag ctgctgttca tgggaaattc ccagaagaat
     9121 tggttgcggt attgaaaaaa gatggggttt tgtggcagtc aacgccagaa gaattggctc
     9181 ttattgctga gaatagagtg gattatttgg gattgaattt ttaccaccct aagcgcgtga
     9241 aggctccgga tgtcattcca gtgattagcc catcttggag tcctgaatgg tattatgacg
     9301 cctacctgat gcctggccgc cgcatgaacg tggataaggg gtgggaaatc tatcctgagg
     9361 ctgtctatga tattgctgtc aagatgcgag accattacga caatatccct tggttcttat
     9421 ctgaaaatgg tgtagggatt tcaggagaag accgttaccg tgatgaaact ggtcagattc
     9481 aagatgatta tcgcatccga tttttgaaag aacatttgac ctacctccac aaaggaatag
     9541 aagcaggctc gaattgtttt ggataccatg tatggacgcc aattgatggc tggtcttggc
     9601 tcaatgccta taaaaaccgc tatggccttg tggaaaataa tatccatacc caagtgcgac
     9661 gacctaaagc ttcagcttat tggtttaaaa aagtagctac tcataaccgc ttgatttcat
     9721 tagaagtaat ggaggagttt ggaggctctg cttctcacct cagtgattga acgtgtgaac
     9781 gtctcctttg agacaagtga ataatgatgt taccaatgat taaacaaggg cctttttcct
     9841 catgttaaga ggatcgaggc tcttgtattt ctttggaagg tgttggtatt tgccgaacta
     9901 gatataagcg ttctgtctgg tttggcatca tctatccatc ttgggtaatt tggctcttca
     9961 attctttaga gtatcttggc taaggatgct tgtttttcat cttggtattt taggattttc
    10021 tttcatattt ttggaccaga taaaagcccc tcatggtttc tagtgactgt ttgttatgtc
    10081 actgtccacg ataaggggac tatatgagtg agttattaag tgaggctttg cttattggcg
    10141 ataactagca tagcttctaa tagctcgagt attaactgat gaccaatgcc tttgacagaa
    10201 gcgtcagcaa caacgacatc aacaaaggga ccaggcatca ggctgcgttc atcagcagag
    10261 agtcatttag gcagaaagcc agtatcgttg tagctaatca gcaatccttt taggatgtct
    10321 ttgtagatgt cagaggcgat tagggacaat aggggataag agggatttag cggtccatgg
    10381 ttaggcctcc ttgtgttctt ggtaaaaatc aatggcagct ccaagaagat tggcttcgtt
    10441 tttaaagtga caaatggcta attggggtct aaggtcggag atttcgacgg cttggtagag
    10501 tttgtctagt tcagcttcaa tcgctgacag tagaaagtca gcttgggaaa caccaccccc
    10561 gattaaaatc agttgaggat caaaggcgtg ttgaatgtta aaaataccga tggctaaact
    10621 ttggagaaag acctgccttt ctttgagtgc taggggatct ccttgttctg tgagggcaag
    10681 gacagctttg ccagtataat cttttccgtt attgacgatt gcactatagc gcttggccat
    10741 attcacaaca gtgcctaatt gactaaaggt ttggtagcgg tcgttcatga tcatgaaacc
    10801 aaattcgccc ccaaataagt gggcaccatg gtaaatcttg ccatcaatga ccaagcttcc
    10861 tccaactcca gtaccaataa cgagcatggc taggctacta gcgccttttc ctgcccctaa
    10921 ggtagcttca gctaaggcgg cacaattggc atcattttcg atagaaatag ggtaatgtag
    10981 ccgttcttct aaggcctctt ttattttaaa atggtgaatg tagggaatag cgctggcacc
    11041 ttcgataata cctttttcct tgttaaccgc tccaggagca ctaacggcaa tacctgagaa
    11101 atggtaagca gagtaacgag caacttcttg gtcgagtaat tggtaaaact tttccagact
    11161 agagggagtg ggaaagctgc tttgctggct taattggcca ttgtgacaga gggcaaattt
    11221 aagggaagtg cctcctatat caatacataa caagctcatg aggtgttctc ctttattata
    11281 gtagaataga cgatcatgtc tcatcttagt atgacagatt taaacaacaa tgaaaaggct
    11341 ttctaagcga ttctatactt tgtgttatca tacctataat ttgttgcttt caaataatga
    11401 aagcgcttga tatactgtaa ctataacgtg attaacacaa agggaggttg ggaaatgaca
    11461 agacaaacca acaaaaagac atccttttgg aaaaatgtga ttaaataccg ggccttatta
    11521 ttaatggtat taccaggttt catctggttc atctttttct tttatattcc agttttggcc
    11581 aatgtggttg cttttaaaga ttttcattat tcagcagggg gctttatgga aagtttgaaa
    11641 gagagccctt gggttggttt ggctaacttc aaatacctct ttgcttctaa agatgcttgg
    11701 ttgattacca gaaataccat cgcttacaat gtgattttct tactctttaa tgttttcttt
    11761 gcgattgctt ttgccattat catgagtgag ttacgaaata agcgaacggt taaggtctat
    11821 cacaccatgt ccttattgcc ttatttctta tcatgggtgg ttattgaata ctttgtttct
    11881 gcctttttaa atacggataa aggtttcatt aatcagctct tgactggaag tggagcagat
    11941 ccaatcaagt ggtattccaa tcccacttgg tggcccttga ttttactctt tatgagtgtc
    12001 tggaaagggc taggctacaa tagcattatc tactatgctt cagtcaaagg gatttctgat
    12061 acttattatg aagctgctat ggtggatggt gcaagcaaat ggcaacaaat taggaacatc
    12121 accattccac aattattgcc aatgatgtct atcctattga tcattaatat tggtaatatc
    12181 tttaaatctg atttcggact tttctacgtt attcccaaaa attcaggccc actttatgac
    12241 gtgaccagtg tgttagatac ctatgtctac aatgccttga ctgctacagg agatattggg
    12301 atggcatcgg cagctagcct ttatcagtca gtagttggga ccagcatctt gttagtcacc
    12361 aatgcgattg ttcgtcgcat ggaccctgat gcagccttgt tttaggagga aaacatggag
    12421 aaaaagaaaa aagtcgaaaa ggttaatgtt agaacctttg atcgaaaaac caatgctatc
    12481 tttaatattc tgattggtct gtttgccatc tcttgtatca ttccttttat cttcgtgatt
    12541 attatttcct tcactgatga aagttacttg attaatcatg ggtatagttt ttttccagat
    12601 gtctggtcaa ctaaggctta ccagtatatt tttcaaggag ccatgtccca tagaatcatg
    12661 aggtcatttg ggatatccgt gtttattaca gtggtgggaa cctttattaa cacgaccatg
    12721 acatcaacct atgcttatgc gatttcaaga ccttatttcc catacagacg tttttttact
    12781 gtttatgcac ttatcaccat gctctttgca ccagggatgg ttgctaatta cttggtggtc
    12841 agcaatctcc ttcatttgaa ggatacggtt tgggctttga ttttaccaat ggctctgggc
    12901 ccatttggca tcttggtcat gagaacgttc tttaaaaaga cagttccaga tagcattatt
    12961 gagtcggctc gtatggatgg ggctagtgaa tggatgattt ttatgaaaat tgttctccct
    13021 ttggctgttc cagggattgc taccatcagt ttattttctg ccttaactta ttggaatgat
    13081 tggtttaacg ccttgcttta tgtgcaaagt gaaaacctct acccaatgca gtacttactg
    13141 atgaaaattc agagcaacct acaggccttg gcacaaaatg ctggcatgag tgcacaaatg
    13201 gcagatagct tagcatcgct gccaaaagaa tcagttcgta tggctatcgt ggttattgca
    13261 accttgccga ttgccttgac ttacccattc ttccaaaagt actttgtcgg tggtttgacc
    13321 attggtgggg tgaaggaata ggacttaagg aactggtcac aagaccagat tttatctaat
    13381 cattagtaac aaaagaaaac attttttagg aggcttcact tatgaaaaag tggcaaaaaa
    13441 tcgtgtgtgt gactggaact gtgcttgcag cgtctagttt agcagcctgc gaaagcaagt
    13501 cagcatcaaa ggatagtgat gtcaaattat tgatgtacca agttggtgac aaacctgata
    13561 acttcgatga attgatgaca attgctaaca agcgcatcaa agaaaaaaca ggtgcaacgg
    13621 ttgaccttca atacatcggt tggggggact gggatgataa aatgagtacc atcattgcct
    13681 ctggtgaaaa ctacgacatt gcttttgcca ataattatgt ggtcaatgca caaaaaggtg
    13741 cttttgctga tttgacaacg ttaatgccaa aatacgctaa gaaaacgtat aaaaacttag
    13801 acccagccta tattaaagga aatactattg acggtaaact ctatgccttc ccagtagatg
    13861 ccaacgttta tgcccaacag atgctttctt tcaataaaga actagtggac aaatatggcc
    13921 ttgacatttc aaacattaag tcctatgcag atgctgaaaa tgtcttgaaa caattccacg
    13981 aaaaagaacc aaatacagca gcttttgcta ttggtcaagt ctttagtatg tcaggtgact
    14041 atgactaccc attaaccaaa acccaaccct ttgctgtgaa aattgatgaa ggcaagccaa
    14101 ccatcattaa tcagtatgaa gatgagtcct ttaaaaacaa tctccgcttg atgcacaaat
    14161 ggtataaaga aggtttgatt ccaacagatg cagcgaccaa tacagaaggt tatccccttg
    14221 aaggaaacac ttggtttatg cgtgaagaaa cccaaggtcc tatggactat ggcgatacta
    14281 tcttgaccaa tgctgcagga aaagacatcg tgtctcgtcc attgactaaa ccgctaaaaa
    14341 ccacatcaca agcacaaatg gcaaactttg tggtatcaag cgtatctaaa aacaaagaaa
    14401 aagcagttga agtcctttct cttcttaata gcgatcccga attgttaaat gggcttgttt
    14461 atggtgtcga aggcaaagct tgggaaaaaa ttgacgacaa gaaaatcaaa ctgctcgatg
    14521 gctatcaacc aaaaatgcac atgggtgctt ggaatactgg taacaataaa atcctttaca
    14581 ctcaagaatc tgttactgac gacatgattg ctaaacgtga ccaatcaatc aaggatgcta
    14641 aagaatctcc aatccttggt ttcactgttg acacaaaagt tatcaaaacg gaattgtcaa
    14701 atatttcaaa tgtcatgaac cgatataaag caagtatcaa tactggtaca gtggatcctg
    14761 acgaagccct tccaaaactt cttgcagacc tcaaaggtgc tggttgggac aaggtgcaaa
    14821 aagaagttca aaaacaattg gacgactttg tagctaaaga caagtaaggc attcattttc
    14881 ttgattataa gttgagggct gggaaatctc agctcttttc ttctgatgag tggaaaaaac
    14941 ggatcatcaa ataagtggaa gttataaagg gagaaaaatt aatgtaaata atgttactac
    15001 tcttagtaaa gcggtttcca aaagcctata aagtgtgcta tattagaaga gtctaaacta
    15061 ttaatcagag taaaactaat accagcatct tactgggggt gctaaaacta gtctcgtagc
    15121 tgctggtcct tgaaaccgct ataagtgtta acaagaaaaa gaggactatc atatgaatgg
    15181 cattgaaaaa acctgctttt taatttggaa aatgatccaa ttgaccctca tttttcatct
    15241 cttgtcctta gctggactga ttgtgtttgg ggtggggcca gcctggcaaa ccattgtcac
    15301 cctattttta aaaacacctc aagaagaaaa ccactattct ttgaaaaggg ctgttcaact
    15361 ttggaaatct tatttcaaag aagctaatct aagatttggt ctttttgccc tgactttcct
    15421 ttttttaacc tttaatttgc attgggcagt acagtttcca tccttgtttt ggtttaccgt
    15481 gtctttcttg attgtaatag ccatggtttg gctaaccatt acctatcttt acatggtttt
    15541 ttatgcagtg agctatgaga ttaacctttg gaataatatg aaattagcct ttatcagcgt
    15601 ctttttaagt ttcaaatcct ttttactcat gctgagtgtt ttgctgggaa ttaccttagt
    15661 gacttggcaa tacaaaggcc tttatctctt tttaaccttc ggagctctag tcttttgctt
    15721 ggactttgtc actaaggcta atcgtcgact agtagatgga gtgattgatg agtgttaaaa
    15781 cattgggaag gtatgtgact aaccacttta agcagggttt tttgattaat cgactgatga
    15841 aattatatag cctgctaatt gtaagttttt tcactttagc agctattggc tttagcagtt
    15901 attctattag tagtacttat aaaagggtgg atgctgaggc gcagatgcga ctggaggaga
    15961 ctctaggtag attgcagtca caaaacgata ttaccctacg ggttttagac cagttggttg
    16021 gccgttcaga agattaccat aatctctatc aatacatgac gttaacccca aaccaatatt
    16081 tttcaaacgt ttttgaggac tgggaaaaag gaaaagacac ggtcctattt tctgaagagg
    16141 tgaggcgtct ttttgacttg taccctgatg tgacttctat gaccctactg ctagaggaca
    16201 gtagtgctta tctgtacgcc gataaggtca ttaagacggg tcgtttgctt tatagcaagc
    16261 ctgaaaaagt aaccggaaat gttttagttc gctctattcg aaatccagaa tcaggagatg
    16321 tgacaggtcg tctttacctg acctttgaca aaccaccgac tttggtagag acacagcagg
    16381 atcactattt agctactttt gcttttgatt actatggccg caaactcttc catcaaggag
    16441 gtcggagatt tcctcattta gaggctgagg ttaaaaaggc tatcaaggca gatcgggcag
    16501 ctgatcttag taatctcagc aaggcttatc ggatgcagta caatcgctcg ggagacctct
    16561 tagcttatgt ggctgtcaga aaatcctatt tattagcgga agctgtcagg acagtttttg
    16621 tgtatggcct tgtttctcta ctcttagctt ggctgttatt gcagttgctc tttagggttt
    16681 tccgaaatta tatccagcag gtttctgaaa ttacggatac tgttgaaatg gttgcagcag
    16741 gcgatttgtc tttaaccatc gacaacagcc acatggaatt ggaactgtat catatctcag
    16801 aagccattaa tcagatgttg gccagcatta aagcttatat tgacgaagtt tatgtgttag
    16861 aggtagagca acgagatgcc caaatgagag ctctgcagtc tcaaatcaac cctcattttt
    16921 tatacaacac gttagagtat attcggatgt acgcccttag ttgtcaacaa gaagaattag
    16981 cagatgtcat ttatgccttt gcgagtctgc ttcgcaacaa tattagccaa gataagatga
    17041 ccaccttaaa agaagaactg gctttttgtg aaaagtacat ttacctttat caaatgcggt
    17101 atccagatag cttcgcttat catgtaaaaa ttgatgagag tattgctgac ttagccattc
    17161 ctaaatttgt cattcaacct ctcgttgaaa attattttgt gcatgggatt gattatagtc
    17221 gccatgacaa tgcactaagc atcaaggctt tagatgagac ggatcatctc ttgattcagg
    17281 tgcttgataa tggacgtggt attagtcaag agcgcttagc agatatggaa aaaaggcttc
    17341 aagatcacca aacaacaggc aatagctcta ttggtttgca aaatgtttac ctccgtctct
    17401 ttcatcattt tcgagacagg gtttcttggt ccatggctaa ggagccaaat ggtggcttta
    17461 tcattcaaat taggattaga aaggatgctt gatgtataag gtactattag ttgacgatga
    17521 atacatgatt ttacaagggc tcaccatgat tattgattgg caggcacttg gatttgaagt
    17581 ggttcaaaca gcaagatcag gcaaggaagc cttagcctat ttgactcagt acccagtgga
    17641 tgtcatgatt tcagatgtta ccatgcctgg aatgacagga ctggatttaa tagaagcagc
    17701 taaaacctat catcctcagc tacaaacctt gattttatct ggttatcaag aattctccta
    17761 tgtgcaaaag gctatggaat tagaaaccaa ggggtatttg ctaaaacctg tggataaggc
    17821 agaattacag gctaaaatga aacaatttaa agactggttg gatgcccagc aggcagaatc
    17881 aatccggcaa gaagcctatc atgacagcct tttgaccctt tggcttacag atgaattgag
    17941 tgagaaagaa tttcagcagt taagtcaagg cttgccagcg gctgctttaa ctggttttac
    18001 agtgctgtat ttggactgtc aagagtggca agcagccatc gatgcttatt ttgaacgaga
    18061 aggtcagcct ttttatctca aaaaagaaga agataagaag gtttatctgg ctgtcctact
    18121 tggtaaaact gatcaagcca aggcgtttac ctatgatcta caaaagcgat tctcaagaaa
    18181 catgcatcaa cttattttgg gagaaacagt tgatgactgg gaaaatgttt atgaaagcta
    18241 caatcaggtg cttaagagcc tcttttacaa ttgtgaagtg tcaccaatta gagcaaaaga
    18301 taagtccaaa attgaactac cagagtcccg tttgcagttc tttgctttta acaaggcgtt
    18361 gatgattggg gacgaaccta caatcttgtc taaattagaa gctatttttg acgagatgaa
    18421 gaccttgaac ttttcaccag aggatgtgaa gcatgtttcg ttcttgttgt tttctgacat
    18481 ttaccgtcaa tttcccattt tagataaaat gacttatctg tccatggtaa aaactattca
    18541 tgacagccaa tccattgatc gcattttgag ggaactaaaa aaagtgttgg atgtcaccaa
    18601 tcagaacaat tcccctgaaa aacgctattc agatttagtt agtgaaacca ttgattgcat
    18661 tcgcaaggaa taccatcaag aattaaccct taaagctatc gctgataggt tacatgtcaa
    18721 tggggtttat ttagggcaat gctttaaaaa tgaaaccgag cgtagtttta cccagtactt
    18781 aaatcatgtt aggattcaaa aagcgcaaca gttattgctt tataccaacc aatccatcaa
    18841 tgaaattgct tatgaaacag gttacaatac caatcattat tttatcaaga tgtttaaaaa
    18901 gttgaatggt ttatcgccaa aagagttcag agatcgttac aaggacaatt accaagccat
    18961 caagggcgac cagtaacgtt aaacaggaga ttgatatgac accagatagc aaggagcaga
    19021 cagtgactga ctaccaatac cactatattg atcaagaagg aagaaagcag ccctttaatc
    19081 aaggctggcg ttttcttatg gcagatgtga cgtgtgctca ggacccttct tttgatgatt
    19141 cgaactggca ggtgattcat ttaccacatg atttcagtct tacgcagcct tacacaagaa
    19201 atggtgaagc tgaaagtgct tataaattag gtggtgtcgg ttggtatcgg cattatctcg
    19261 tgttagatga ggttttagct ggttgtcatg ttgccattac ttttgagggt tcctatatgg
    19321 aaacggaaat ttatgttaat gggcaattca ttggcaaaca cttaaatggc tatcaggaat
    19381 ttacctatga tatctctgat gtagtgactt ttggagctga aaatcttctg gctgtccggg
    19441 ttgaaaacaa ggttcccagt tctcgttggt attcaggcag tggtctttat cgtgaagtga
    19501 gtttgtctgt tcttccccag cttcattttg tggcagacca ggtagcaatg accttggctg
    19561 atactgctgt gcaggagaaa gggcaacaga aagttgattt acgctttgcc cttaatcagt
    19621 ctattcagac ttgccactac caattgagcc tctgtttatg ggaacaatca cattgctcaa
    19681 aagacaagaa actcctttat caagaaacag aagtgcctct agctgatttg gctttccaaa
    19741 gacagtatgg tcttacactg tctttggaag aactccaatt gtggtcacca gataatcctc
    19801 acctctacga tttagaactc actctttact atcaaggtca ggtcattgat tgcttttgcc
    19861 ttgaaactgg ttttcgtcag ctgactttta tggccaatca ggggcttttt gtgaatggca
    19921 gagctgtcaa attaaaggga gtttgtttgc atcatgacca aggaggccta ggagcttgtg
    19981 cttacgaaga tgccctagcc agacaattag tcctgttgaa agacatgggg gctaatacca
    20041 ttcgtagcac ccataaccca agtagtccca aacttaggca actagccaat cgtctcggct
    20101 tttttgtgat tgaagaagcc tttgacacct ggacctatgc caaaaatgga aacgttaatg
    20161 acttttcaaa ctatttccat cagactatcg gaactgaaaa tgcaaactat cttcaaagag
    20221 tgaggtcacc tgaaacgagc tgggcccagt atagtattga ggccatggtc tggtctgcca
    20281 agaatgaccc gtcagtgttg atgtggtcga ttggtaatga attgatggaa ggtttttcag
    20341 ccgatgtcag tcattaccca gagctgacaa gacaaatgtg ccaatggatt gctgctattg
    20401 acacctcacg tcccattacc tttggggaca ataaattaaa agaagctgac ttttgctggc
    20461 atgaggaagt gtcgcaaatg gcaactcttc ttagtcaatt agaccatccc cagggactca
    20521 ttgggttaaa ctatgctaat ggaaaagact atgataggct tcatgaggaa cactctgatt
    20581 ggctgttgta tggttctgaa acagtctcag ccattaccag cagggcttat tataaagaga
    20641 caaaaaaagt tcttgactcc ggttatcacc taacctctta tgatcatgct aaggttgatt
    20701 ggggtgcctt cgctagccag gcttggtatg ataccattac ccgtgatttt gttgctggcg
    20761 aatgcgtttg gacaggcttt gattatttag gagaacctac cccctggaat aagatcgaca
    20821 gcggtgttgt aggcctttgg ccttctccta aaaatgccta ctttggcatt ttagacacgg
    20881 caggctttcc aaaagatagc tattattttt accaaagtca gtgggcgcag gggcagacca
    20941 ccttgcacct gttaccagtc tggcagaagg accagctttg tttcgatgag cagggattgg
    21001 tggaggttgt ggtttatagc aatgctgcca gtgtgcaact gatgtttgaa gatgagcagg
    21061 gcaacttaac agattatgga aggaaggcat ttcatactta tagcacgcca acgggacaca
    21121 cttatcagct ttaccaagga gctgacgctg ctaagaatcc tcacgagaat ctttacctga
    21181 cttggcgagt gccctatcaa aaagggttgc taagggcagt ggcctacgac atttctggta
    21241 aaagcattcc aaaaaccagt ggacgttctc aggttagaac ctatggttca gtggcaaagc
    21301 tatcttggaa agcctttgaa gcacctattg acgctccttg ggaattgctt tatctggatt
    21361 tatcactctt ggatagtcga ggtgaattgg tcagccatgc tcaagattgg ctaaaggttc
    21421 aagtagaagg accagcccgt ttgttggctc ttgataatgg gaatccgact gaccacaccc
    21481 cttatcagga gcctctacgc caggcatatg ggggaaaatt attagccatt ttagccctca
    21541 ctggagaggc gggacacatt aaggtcacgg caaaagcctc gcagcaactt agcagtgttt
    21601 ttcagacaca agtagcctgc cgtgccccca aagaacactt agtgacatta cctttagaga
    21661 aatatcagtt taaaaagagc cgactgacaa gtcaacaaga aattggtcaa tcttgtctaa
    21721 agaccacaca attcctttct gttttggaga acaaagacac ccagcaaaaa gcaggtaagg
    21781 ctagcacacc tcacattctt atcaccagtt accagcacta tttacctctg cccttgcagg
    21841 tttgttctca ggcatttgat tttaaaaaag gcgatgatat cttcttgcct cagtggttcc
    21901 aagtcaaaga tgcccaaggt tcttttgtga aaaaagcttt tccaatgaca tggtacctaa
    21961 aagagaaaat ctttgccgag cctattcttg tcacgggcta tgtttcttgt tttgggcaat
    22021 caataccggt tgaggcaagg ctcaacctag cacaagctag caaaaggcgg caccaggatc
    22081 tcagtttagt cgccaagcaa tccctggccg caagtcaaaa aaggctaact tattgctacc
    22141 aatatgatac ggtgcaagcc ataggagcca ttagcttgca aaatcacaca ggcacatctc
    22201 ttagtgtttc tgtggcaatc agttatggta gtcagagctc tattaagcaa gaaaccttga
    22261 tgctagctga tagccacttg agccagttag aagaggttat tttcttcgat gatattttga
    22321 ctgttttgta tctggcagtg acccttgaag tcattgaagg acctgaccta actgaagaca
    22381 gtattcaaat tggcctttgg gatttagcat gaagcatgag taaaagaaga cttctttggc
    22441 accaggtctg atgaattctg taaaataagg tataataaaa aacaaatagg cgtcctttat
    22501 tacgtgaaag gcgcctttac aaggttgaaa tggaaagtga gatttctaaa tgtcagaacg
    22561 tttatcaggc cacaccctgt tggtctcttt gcttgctacc cctattcgtc atagtttgtc
    22621 tcccaaaatg cacaatgaag cctatgctaa acttgggctg gattatgcct accttgcttt
    22681 tgaagtaggg acagagcagt tagcagatgc cgtgcaaggg attcgtgcct taggaattcg
    22741 tggctccaat gtgtctatgc caaataaaga agctatttta cccttattgg atgacttgtc
    22801 cccagctgct gagttggtcg gtgcagtcaa tacggttgtc aataaggatg gtaaaggaca
    22861 tctcgtgggg catattacag acggcatagg agcgctcaga gccttggctg acgaaggagt
    22921 gtcggtaaag aacaaaatca ttaccctagc aggagttggt ggggctggga aagctatcgc
    22981 tgtccagctc gcttttgacg gcgccaaaga ggttcgtttg tttaaccgac aagctacacg
    23041 tctaagtagc gttcaaaagc ttgtgacaaa actgaaccag ctaacaagga ctaaagtaac
    23101 ccttcaagac ttggaagatc agactgcctt taaggaggcc atcagggaat ctcatctatt
    23161 tattgatgct acaagtgtag gaatgaagcc tttagaaaat ctaagtttga ttaccgaccc
    23221 cgagctcatt cgcccagact tggtggtctt tgacatcgtg tacagcccag cagaaaccaa
    23281 gttactagcc tttgcccgtc aacatggcgc ccaaaaagtc attaatgggc tagggatggt
    23341 gctttatcaa ggagcagaag cttttaaatt aatcactggt caagacatgc cagttgacgc
    23401 tattaaacct cttttaggtg atgagtaaca ctttttaagt aagcttaatt atgatatatt
    23461 aataagagat ggagcagtca tcatgggctg ctaaaattct ttttgcgaga gctgttttta
    23521 agagcaaaaa gcttatttga agagccttat ccctaacgtg atacaagtca tagttcctgt
    23581 gaggtctgcc agataaccta ccgttttttt agaaaagaag ctcttataaa aggaaaagtt
    23641 caatgaataa aggagaatag caatggtaac aaaggaagtt gggcatgact ttttagcacg
    23701 tctaggaaaa aaacgcttaa gacctggcgg tgttgaggcg acaaactggt tgattgaaca
    23761 aggacaattc tctcaggatt gtcacgtttt agaggttgcc tgcaatcgct gcacgacagc
    23821 aatagagtta gccaaaacct tcggttgtca gattgatgcc gttgacttaa accccaaggt
    23881 tgtcaaagaa gcacaagaaa gggttgctgt tgaggggtta acccgtcaca ttacagtatc
    23941 ccaagccaat gctttatcac taccatttcc agataaccat tttgatattg tgattaatga
    24001 agccatgtta accatgttga acgcccaagc caaggagaag gcactcaatg aatattggcg
    24061 tgtgttaaaa ccaggcgggc gacttttgac acatgacgta gcttatgaag atccaaaaac
    24121 aagacaagta cttgacgagt tacgtcaaac gattcatgtc aatgttgctc ccttgtcact
    24181 aacaggttgg caagccttat atcaagaaca cggtttttct caagtgactt atcaccatgg
    24241 gaaaatgtct ttgatgacac ctcgtggaat gattaaggat gaaggtctta tgaacaccct
    24301 gaacattatg agacgaggcc ttaagaaaga gaacagacaa cagtttttaa agatgcgtcg
    24361 tttttttacc agaacaggca aaaatctctg ctatattgct gtaaaaagtg taaaataaac
    24421 cttagaccaa cctgtccata caactaaaca gaccatagag gagttcgaag atgtcttata
    24481 tcaataatat tgagcacgcg aaggttcttg atttgacgca agaagtaatg attgagcaag
    24541 atcaaatgtt aagtagaaca ctagtccaaa ggcaagacct aggaataaca gttttttcct
    24601 tagataaggg ccaagaaatt gggcgacact catcaccagg agatgccatg gttactatct
    24661 taagtggctt agcagaaatc accattgatc aagagaccta ccgggttgct gaaggtcaaa
    24721 ccattgtgat gccagctggg attcctcatg ccttatatgc agtagaagcc tttcaaatgc
    24781 ttttagtagt agtaaaaccg gaggtttaac atgattgaaa atctctctca actggtgcaa
    24841 tatcatgatc atcaggtggc tagccgatcc ttgagccgtc tactgggatt atctcaatct
    24901 attgttttat atgccatggc ccaaggagaa agcattagtc aagagactag tccaaaagat
    24961 aaagtaatac ttgttttaga aggtcagctc attttcgatt tagaggatca aaaacaggtc
    25021 ctaactcaag aaagtctcat agccatacct gcccaaaaag tccatcattt agaagctaaa
    25081 accgattgta aactattaca agtgctttta gattaagggc ttattgtcaa ctgtattggg
    25141 tggcctatgg aaaagcaaaa gagaagatga cgctcatctt ctctttttac gtattcaaag
    25201 cgatgacaat acgaatttta aggcttggct ttctcatctc cagattaagc cctttagctt
    25261 ggtttagttt agaattcact aaaaaaacct taatgagttt cctatctcct tataggtttc
    25321 tcattaaggc tttttagacg aaataattga acgacaggca aaagaaagat ctttttacga
    25381 gtgataccgt tctcctatag tttcataaat cctctttttg atgttgctgt tagttattct
    25441 tagctaaaag ttatattgtt atcaagactt gtcttgtagt aacatgtttt aggagttcca
    25501 ggataccgtt attctcttag caagcttaac tagctagagt ggagtcacta tttataataa
    25561 actgtcgagc ttctcgacaa attggtgatc gtctttgtta atggctaggg tggtaaaacc
    25621 ggtttctgtg ggtaatataa agataatatg ttgctcagaa ttcttcttat ctgttttaaa
    25681 gacgtcaaag agggatgtcg cagagacatc agctctttca agtgttgttg gtaactggta
    25741 acgttcaaaa acttgaagga ggctatccaa gtcctctttt ggcaagagtc ctttggaaac
    25801 agccagttta gcatcaacca ccataccgat agcaatggct tcaccgtgat ggtaaaaatc
    25861 atggtggcag agcgattcaa tcgcgtggcc caaggtatgc ccaaaattta aagacatgcg
    25921 caaaccattt tcaaattcat cttgttcaac aatatgtttt ttattggaaa tggattggta
    25981 aatcagacgt tcaagtccct gcttatcggt ttctttttga aaatcacgta actgctgaaa
    26041 aagcggtcta tcatgaatga agccgatttt gagcatctca ctgatgccgc aggagaattc
    26101 ccgttgcggt aaggtttcca aaaaggtggt tgaaataatg atagcttctg gagggtaaat
    26161 gctgccaatc atattggtta gacttttaaa atggactcca actttgcccc cgatactgct
    26221 atccacctga ctaagtaaag tcgttgggat ttgaatgtag ggaataccac ggtaataggt
    26281 tgcagcgaca aaaccaccca gatccccaac aacaccacca ccaatagtaa caatcatatc
    26341 ttttttggaa aaattttcag cgatgagctg gtcgtagata gcactgacac gttccaagga
    26401 tttggtttga cctcccgggg ggcagacatg gacaaaagcg ttgtattgct gagcaaattc
    26461 ttcaaagaga gtctgataca agtggtagac cgtttgatcg gtgataaaca agagtttacg
    26521 ctgcttgcgc tcaccaaggc aatctgctaa ggtttttaaa acatggtcgg taaacaggat
    26581 atcataatcc ttgacccgag aatggacatg aagtgtttgt ggcataagca ctcctttcat
    26641 cagtactagg taacagagat aacgcagtga tatttagagc ttaatcttgc caaagttcct
    26701 gccccaactg ttctaattgc ttataatcaa tttgctgggc agcatctgac agcgcatggt
    26761 cgggatcagg atgaacctcc atcatgatgc cattggcacc gacagccttc gctatcttag
    26821 cagcaggtaa gagtaaatct cgtctgcctg tcgaatgaga cacgtccaca ataatcggta
    26881 aatctgtctt ttgttgaata atagggactg ccataatatc aagcatattt ctagtctcca
    26941 catcgtagcc acgaacgcca cgttcgcata agataatgtt agattttcca gtgtcttgta
    27001 agtaggaaag ggcgcccaaa tattcctcga tagtagccat caagcctcgt ttaaacaaaa
    27061 taggcttatc gatgtgtgac aaggttttta aaaattcaaa attttgcatg ttgcgggcac
    27121 caacctgaat gacatcgaga tagtcatagg cttcttctaa ttggcgctcg gacatgattt
    27181 cactgaccga tagtaagcca aattcctgac aaacctcatg caagtaacga attccttgca
    27241 gaccaaggcc ttgaaaagaa gcagcagatg ttcttggttt ataggcgcct cctctaaaat
    27301 agttataacc aagttttttg gcacttgaag ctgctaatct aatgtgatca taagattcaa
    27361 tggaacaagg tccaacaata aaattattct tagaacaagt cttattttga aagtcagaca
    27421 taatgtcaat cattcgtttg attcctttac taattctgta tggcgtgctc tatgggataa
    27481 cgtcttattt aggaatacaa aatctcgttg ttatccatga agcaccctta gcctgtgaaa
    27541 tgataccata aacaagatga aatagcaagt aaaccctaga ttgtctatgt tcttagtcat
    27601 ttttattaaa aaaggcttgt tagctataaa aaatactgcc ctaaatctta ttagggcagt
    27661 attttttata gatgccacca ccgagaatcg aactcggaac gaagcattac cattgcttta
    27721 ttataccatt taactacagc ggctaccttt ttataatact ataagttatt aattgatgca
    27781 agcttttctt aaaaatattt ccttaattgt ttatcatcaa aatgagagta ccagt
//
LOCUS       DATURV010000018        27757 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DATURV010000018 DATURV010000000
VERSION     DATURV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27757
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.018"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..1140
                     /locus_tag="VCF75_000751"
     CDS             <1..1140
                     /locus_tag="VCF75_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEP2546331.1"
                     /translation="FVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNRPIGTAYIS
                     KQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQEGDGFGGV
                     TIDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDTETAHLYGE
                     LAQETFSILENGIAYQVFLNEGLMTGIFLDQHDVRRALVDGLAMGKSLLNLFSYTAAF
                     SVAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYFKYAKRKKL
                     IFDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAANMTVSQFK
                     KQIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            1137..1823
                     /locus_tag="VCF75_000752"
     CDS             1137..1823
                     /locus_tag="VCF75_000752"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEP2546332.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVALNDMKRI
                     IEVLEMDISN"
     gene            1917..3083
                     /gene="aroC"
                     /locus_tag="VCF75_000753"
     CDS             1917..3083
                     /gene="aroC"
                     /locus_tag="VCF75_000753"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEP2546333.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            3144..3485
                     /locus_tag="VCF75_000754"
     CDS             3144..3485
                     /locus_tag="VCF75_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEP2546334.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADVSASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3706..5058
                     /gene="gorA"
                     /locus_tag="VCF75_000755"
     CDS             3706..5058
                     /gene="gorA"
                     /locus_tag="VCF75_000755"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEP2546335.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKEKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFSLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(5146..6414)
                     /locus_tag="VCF75_000756"
     CDS             complement(5146..6414)
                     /locus_tag="VCF75_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP2546336.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(6444..6884)
                     /locus_tag="VCF75_000757"
     CDS             complement(6444..6884)
                     /locus_tag="VCF75_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546337.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSTTEGTQLPNATGAEDANTGLPTNP"
     gene            7119..8261
                     /locus_tag="VCF75_000758"
     CDS             7119..8261
                     /locus_tag="VCF75_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP2546338.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPVRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     VYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            8273..9487
                     /gene="thiI"
                     /locus_tag="VCF75_000759"
     CDS             8273..9487
                     /gene="thiI"
                     /locus_tag="VCF75_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEP2546339.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVR
                     SDRDRTHVYLNGTDYQPVVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQSFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            9525..10817
                     /locus_tag="VCF75_000760"
     CDS             9525..10817
                     /locus_tag="VCF75_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEP2546340.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNELAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    10922..10996
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            11031..11345
                     /gene="rplU"
                     /locus_tag="VCF75_000761"
     CDS             11031..11345
                     /gene="rplU"
                     /locus_tag="VCF75_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEP2546341.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            11357..11683
                     /locus_tag="VCF75_000762"
     CDS             11357..11683
                     /locus_tag="VCF75_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEP2546342.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            11711..12004
                     /gene="rpmA"
                     /locus_tag="VCF75_000763"
     CDS             11711..12004
                     /gene="rpmA"
                     /locus_tag="VCF75_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEP2546343.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            12362..13276
                     /locus_tag="VCF75_000764"
     CDS             12362..13276
                     /locus_tag="VCF75_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP2546344.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLSDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            13273..13731
                     /locus_tag="VCF75_000765"
     CDS             13273..13731
                     /locus_tag="VCF75_000765"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEP2546345.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            13721..14611
                     /locus_tag="VCF75_000766"
     CDS             13721..14611
                     /locus_tag="VCF75_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP2546346.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDTLAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGYGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            15006..15527
                     /gene="pyrR"
                     /locus_tag="VCF75_000767"
     CDS             15006..15527
                     /gene="pyrR"
                     /locus_tag="VCF75_000767"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEP2546347.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            15543..16802
                     /locus_tag="VCF75_000768"
     CDS             15543..16802
                     /locus_tag="VCF75_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEP2546348.1"
                     /translation="MKDIIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKAIGNDWIDNILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            16863..17798
                     /locus_tag="VCF75_000769"
     CDS             16863..17798
                     /locus_tag="VCF75_000769"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEP2546349.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            17842..18924
                     /gene="carA"
                     /locus_tag="VCF75_000770"
     CDS             17842..18924
                     /gene="carA"
                     /locus_tag="VCF75_000770"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEP2546350.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            19170..22346
                     /gene="carB"
                     /locus_tag="VCF75_000771"
     CDS             19170..22346
                     /gene="carB"
                     /locus_tag="VCF75_000771"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEP2546351.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPALDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQELQMHVDHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLTIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
     gene            22521..23792
                     /locus_tag="VCF75_000772"
     CDS             22521..23792
                     /locus_tag="VCF75_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP2546352.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGVGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVTVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            23792..24502
                     /locus_tag="VCF75_000773"
     CDS             23792..24502
                     /locus_tag="VCF75_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546353.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            24514..25734
                     /locus_tag="VCF75_000774"
     CDS             24514..25734
                     /locus_tag="VCF75_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546354.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGKGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            25988..27721
                     /locus_tag="VCF75_000775"
     CDS             25988..27721
                     /locus_tag="VCF75_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEP2546355.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SRLVRIISSLY"
BASE COUNT         8698 a         4631 c         5897 g         8531 t
ORIGIN      
        1 tttgtcgaaa agaagctgac agcaggggta caattattag atgaaaaaga tttttctaat
       61 ataaaggaaa aaaatcagtt ggttcaactg gtgaccaagt ccaatcgtcc cattggaaca
      121 gcatatatct ctaagcaaaa taaaggaatt ggttggtatc taggaccaga aaaaatcgac
      181 ttgtctatct cttattttgt tagtctattt tcagttgcta aggccaaaag acaagatttt
      241 gctcaatcgg acgagaccaa tgcttatcgc ctttttaatc aagaaggaga cggttttggt
      301 ggagtaacaa ttgatcttta taaagatttt gcagtctttt catggtacaa tgcctttgtc
      361 tatgacaaaa aagaaatgat tatggaggct tttcaacagg tttttcctga agtcaaagga
      421 gcttatgaaa agtgtcgctt taaaggtcca gatactgaaa ctgctcatct gtatggtgag
      481 ctggcacaag aaaccttttc gattttagaa aatggcatag cctatcaagt cttcttgaat
      541 gagggcttaa tgacaggtat ttttctagac cagcatgatg ttagaagagc actagtagat
      601 ggtttagcta tgggcaagtc ccttcttaac ctattttcct atacagctgc cttttctgta
      661 gcagctgcga tgggtggtgc tatcgagacc acttctgtgg acttggctaa acgatcgaga
      721 gaattatcac tagctcattt tgagcataac cagttaaact tagcctcaca tcattttgtg
      781 gtgatggatg tttttgaata cttcaaatat gccaaacgga aaaaattaat ctttgatgtg
      841 attgtgattg acccaccaag ctttgctaga aataaaaaac aaactttctc tgtgagcagg
      901 gactaccaca aattaatcac agaggccttg gacatattga gtcctaaggg aactattata
      961 gcttcaacaa atgctgctaa tatgactgtt agccagttca aaaagcaaat tatcaaagga
     1021 tttggaagtc gtcgtcctga gtctatgact ctccagcagc ttccgagtga ctttactatc
     1081 aataaagcag atgaaagaag taactattta aaggtattta ctataaaggt acgagaatga
     1141 gaattgtagc accagtcatg ccaagacatt ttgacgaggc tcaagccatt gatatttcta
     1201 aatatgaaga tgttaattta attgaatggc gagcagactt tcttcccaag gatgagattg
     1261 tcgcagtagc accagctatt tttgaaaaat ttgccggaaa agaaattatc tttacccttc
     1321 ggaccgttca agaagggggg aatattaccc tttcaagtca ggagtatgtt gatattatca
     1381 aagaaatcaa tgctatttat aatccagact atattgactt tgagtatttt acgcacaagt
     1441 cagtatttca agaaatgctt gattttccaa atcttatttt gtcttatcac aactttgaag
     1501 agactcctga aaatttaatg gaagcttttt cagaaatgac caagttggca ccgcgtgtgg
     1561 ttaaaattgc tgtgatgccc cagagtgaac aagatgttct agatctgatg aactatacca
     1621 gaggctttaa gacgctcaat cctgagcaag aatttgcaac tatatctatg gggaaattag
     1681 gaagattgtc tcgttttgcg ggagatgtca ttggctcttc ctggacatac gtttcattag
     1741 atcatgttag cggtccaggt caagtggctc tcaatgatat gaaacgtatt attgaggtct
     1801 tagaaatgga tatctctaac tagtgaatgg tctaaaaata ttatttctac aatagttaag
     1861 aaatgatgtt tttagattaa tgtttttaaa ataagtggaa ccaaaaagga gaaaaattgc
     1921 gttatttaac agcaggagaa tctcatgggc catccttaac agctattatt gaaggtatac
     1981 cagcaggatt aactcttcat ccagcggata ttgatcacga attacagcga cgtcaaggag
     2041 gttatggccg tggtgccaga atgtctattg agactgatcg ggttcaaata tcctcaggag
     2101 ttcgtcatgg caaaacaaca ggggccccca ttaccttaac ggtaattaat aaggaccacc
     2161 aaaaatggct agatgtcatg gctgtaggag acattgaaga gactctcaag ttaaagcgtc
     2221 gtgttaagca tcctagacca ggacatgcgg atttggtagg aggtattaaa tatcatttta
     2281 acgatttaag agatgcttta gagcgctcct cggctagaga aacaacgatg agagtggctg
     2341 taggcgctgt tgctaaacgt atcttagctg aacttggaat tgatatgtta catcatatct
     2401 taatatttgg tggtataacg atcacaatac cttctaagct ctcttttaga gagctgcaag
     2461 aaagggctct ccattcagaa ttatctattg ttaatccaaa gcaagaagaa gaaattaaaa
     2521 cgtatattga taagataaaa aaagaagggg ataccattgg tggtattatc gaaacgatag
     2581 ttcaaggggt gccagcaggt cttggatctt atgtccaatg ggataaaaaa ttagatgcca
     2641 agctggctca agcagttctt tccattaatg cttttaaagg ggtggaattt ggagttgggt
     2701 ttgatatggg attccaaaag ggctctcagg taatggatga aatcacctgg acacctaccc
     2761 agggctatgg tcgtcaaaca aaccacttag gtggctttga aggtggcatg accacaggac
     2821 aacctcttgt tgttaaaggg gttatgaagc ccattcctac tttgtataaa ccactcatgt
     2881 cggtagatat tgatagtcat gaaccttata aagcaacggt ggaacgctct gatccgacgg
     2941 ccttaccagc tgcaggggtt atcatggaaa atgtggtggc gacggtgctt gccaaagaga
     3001 ttttagagac tttttcttct actaccatgt ctgaattgca aaaagctttt tcggactatc
     3061 gtgcctacgt caagcaattt tagatagcga gatactggct tttatggtat catacaggta
     3121 ttaatgaaca aaaggagact gtcatgtcac aagaaattta tgactatgct aatcaacttg
     3181 aaagagcagt tcgtgctctg ccagaatacc aaaaagtgtt agaagtaaaa gaggccattc
     3241 aagcagacgt tagtgctagc gaactgtttg atgagtttgt cgctatgcaa gaaaaaatcc
     3301 aagggatgat gcaatcaggc caaatgccaa cggctgaaga acaaacaagc attcaagaac
     3361 ttagccaaaa gattgaagct aatgaccagc taaaagctta ttttgaagcg cagcaggcct
     3421 tatctgttta catgagtgat attgagcgta ttgtatttgc tccgttgaaa gatttagtca
     3481 agtaagccaa ttaagatagg aaaaaaacag attgctggca gctcgtcagg atctgttttt
     3541 tagtgtttat tttctttgta tcagaaagtt gagcctcttt cttatttgtg atatagtcaa
     3601 ataaagtgac caaaatagca ggccagtagt ggctttaatg aaggcaagtt gtctgctaac
     3661 tggctatgct aaaacatcag tattagaaag taaaaggtga taaaaatggt tattccttat
     3721 gattatattg ttattggtgg agggagtgca ggtattgctt ctgcgaacag ggcagccatg
     3781 catggggcca aagtattgtt agctgaaggt aaagagattg ggggaacctg tgttaaccta
     3841 ggctgcgttc ctaaaaaagt catgtggtat ggcgctcaag tagcagatat tttagggact
     3901 tacgctaagg attatggatt tgactttaaa gagaaagcat ttgattttaa acaattgaaa
     3961 gcaaatcgtc aagcttatat tgatcggatt catgcttctt atgagcgagg ctttgagcag
     4021 aatggtgttg accgtattta tgattatgct gttttcaaag atgctcatac ggtagaaatt
     4081 gcagggcagc tttataccgc accgcatatt ttgattgcca caggaggaca tcctgttttt
     4141 ccagatattg agggggcaca atacggtatc agctcggacg gcttttttgc cttagatgag
     4201 gtaccaaaac gtacggctgt ggttggggca ggctatattg ctgtggaact tgccggtgtt
     4261 ttgcatgctt tggggtcgaa gacggatttg tttatccgtc acgatcgacc tttaagaagt
     4321 tttgacaaag ccattgtgga tgttttagtt gaggaaatgg cagttggtgg tccttgctta
     4381 caccctcatt cagaagtggc aaaggttgtc aaaaatactg atgagtcctt gaccctttac
     4441 ctcaaagatg gccaagaagt tgaggttgac cagctcattt gggctatcgg tcgcaagcct
     4501 aatctagaag gttttagtct tgacaaaact ggagtgacgt taaatgataa aggttatatt
     4561 gagacagatg cctatgaaaa cacgtctgtc aaaggtattt atgccgtggg agatgttaat
     4621 gggaaactgg ctttaactcc agttgctgtg gcagctggac gtcgcctatc agagcgtctt
     4681 ttcaatggca aaacagacga gaaattggac taccagaacg ttgctactgt gatttttagt
     4741 caccctgtta ttggctctgt tggcttgtca gaagaagcag cagtcaaaca gtatgggcaa
     4801 gaagcagtta aaacatatca atcacgtttt acgtctatgt ttactgctat cactaaccat
     4861 cgccaacctt gtctgatgaa gttggtaaca gtcggagata ccgaaaaaat agttggtctt
     4921 cacggaatcg gatacggggt tgacgaaatg attcagggct ttgcagtagc catcaaaatg
     4981 ggagcaacca aggcggattt tgataacacc gttgctattc acccaactgg ctcagaagaa
     5041 tttgtcacca tgcgctaaaa aatgtagcct aaggcaataa aaaatgatgt taacagtttt
     5101 tgaggaaagt cttctaaact gctagcatcg ttttttgtta ggtgattagg ggaatatgtt
     5161 tttaaagata tttgcgttgc tcagacaata ttgccttacc tcggagataa aataaagtga
     5221 ccctgtaaca acaaagagtt catcagatgt tacggaacct tgtaaccagt ctctgtaatc
     5281 ttttacctgt gggtatttat caggataatc ctctaaaaga tttgcttcag ggaaatcaaa
     5341 agtcgtgaca gatacttcaa aagtatctag ttgagccagc aagtcggcta aaggcttacg
     5401 tttgagacct gcaaagagaa tatgaatagg acgtttggga aattcttctt gcagtagaga
     5461 ctttaattta gcaatggaat ctggattgtg agcgccgtct aatagaagat taggttgaat
     5521 aaattcggac cggccaggcc aagttgttgc ctcaattcct ttttgaatcg tttttggaga
     5581 tattttagga aaggattttc taagtaattg agctgtcata atggctaaac aggcattcgc
     5641 tttttgatgt tgacctaata gtttgagacg gagtttggtt aaattcaagt tatcgtgttg
     5701 aaaggaaaac gtcttttctg aaggattgag caaaaaatct tttcctaatt gatataagcg
     5761 tgaaccggct ttttgggtaa cttgttgaaa gacttgacta gcctcctgct caagttgacc
     5821 gataataacg ggttctttag ctttgataac ttctgctttt tggtgagcaa tttctgctag
     5881 gctataacct aaacgttctt ggtgatcaaa actaatggaa gggcatacga cggccagagc
     5941 atgaaaaaca ttggttgcgt cggttttccc accaatccct gcctcaataa tggcaatatc
     6001 aacaggttta agattggcaa agtagcaaaa cattatgaga gtcactaact caaactcggt
     6061 aggtctatcc caatttgttt caagggacat tttagtgaga atgggtctaa tcacgctcac
     6121 acattgcgcc aattctaggt ctgaaatggg ctggccatta aggcagatcc gatcgtgaaa
     6181 agagatgata aaaggagatg taaaggttcc gacctgataa ccagaagttg acaggatatg
     6241 ctgtaagtag gctgtcacag agcccttgcc gttagttcca acaacgtgaa tagcagggaa
     6301 tttgtcttgt ggccggccta gtgcctcaag taaccaagcc attctggcca aatcggtctt
     6361 gcgaccatta gccttaaagg tatgaatcca ggaaatgcta tcataggtag tcattactat
     6421 ttcttgtcct tctaacaatg cttctaaggg ttcgtaggaa gaccagtatt agcatcttca
     6481 gctccagttg cattaggaag ttgcgtccct tcagtggttg atggtgtctc aggggctgta
     6541 ggagttgtct cgttgtgaga aggtgtggta gaagatgacg gaagagttgt cttattatca
     6601 tctgtggttt ctgaagtacc tgtatggttt tgattaccct gagaagactc tttttcaata
     6661 atcttgacaa tggtattact cgattctttg ggtgtatcat tagaggtctt atctggtaca
     6721 ggctgcagtg ctcttgtaat ggtaatggtt gccacaatga tactcaaaat actaccaata
     6781 agtgcaatgg atttttggaa agctgtaaaa cctgttttaa tatgctcggt agggacacct
     6841 cctgatttac gaggtttttg ttgacgggaa taacgtgatg acatattttt ctcctttaaa
     6901 atcatcgtaa tgccaagatt aaggaggagg aaacagaagt cctagctcct ctttaacact
     6961 tcatctatca gtataatcta gaaaaaaagt gaagtcaacg gtttacctta tgtctaggac
     7021 aaggaagtcc tttgggctta ttgtaatacg aatattcttg atttcatttt agctattttg
     7081 tgatagaata aagtattgag taaaaatgaa ggtgacacat gatttatttt gataatgcag
     7141 ccacaaccat accttatgga gaggctctta aaacttacca agaagtagca acaaaaatat
     7201 atggcaatcc ctcaagtctg caccaattag ggacaaatgc gtctcgcatt ttagaagctt
     7261 cccgtaaaca gattgctggt ttgttgggtg ttaagtcaga agaaatcttt tttacgtctg
     7321 gtgggactga aagtgctaat tgggctatta agggaattgc ttttgaaaaa accgcttttg
     7381 gcaaacatat tattatctca gccattgaac accctgccgt gagtgaaagt gtcaaatggc
     7441 ttctcacaca aggattcgaa gtttcttatg ctccagtgac aacacaggga gttgttgatg
     7501 tgaatgcttt agctgagctg attagaccag acaccatctt aatctcgatt atggctgtta
     7561 ataatgaaat gggagctatc caaccggtta gagccatttc aaacctattg gctaaccaac
     7621 caacgatcac ctttcacgtt gatgctgttc aagccattgg taaaatacca ctctgtgatt
     7681 atatgactaa tcgtgtggat ttggcttctt tttcagggca caaatttcat agtgttcgtg
     7741 gtgttggctt cctttataaa aaagcaggca agcgactcaa tcctctcttg tcaggcggcg
     7801 gccaagagca ggagttacgt tcaaccactg aaaatgttgc aggaattgct agcatggcca
     7861 aagcattaag aatagtcact gaaaaacaag tgagcgtctt acctaaacta acagcaatgc
     7921 gagatgtgat ttacaaagct ttgtccgttt acccagatgt gacagtattt tcagctcaag
     7981 aaggctttgc gcccaatatt ttgacctttg ggattagagg ggttcgagga gaagttattg
     8041 tccacgcctt tgaaaaatat gagatttata tttcgaccac aagtgcatgt tcgtccaaag
     8101 caggtaagcc agcaggaagc ctagtggcta tggggattcc tgtaaaaact gcccagacag
     8161 ccgttcgtat tagtctagac gatgacaacg atatgggaca agtagagcaa tttttgacca
     8221 tattcaaaca gatttatgat aaaacacaga aagttaggta agattaggta agatggacta
     8281 ttcagaaatt atggttcgcc atggtgaact atcaactaaa gggaagaatc gcatgcgatt
     8341 cattaacaaa cttaaaaaca atattcaaga cgtactggct cctttcccag ccattactgt
     8401 ccgttcagat cgtgacagaa ctcatgtcta tttgaatggc acagattacc agccagttgt
     8461 agaagcttta aagctggtgt ttggtgttca agctctatct cctgtttata aacttgaaaa
     8521 aagtgtccct cttttggtaa cagctgttca agatatcatg acttctcttt atcgtgatgg
     8581 actgacgttt aagattgcca ctaagcgtag tgatcatgcg tttgaattgg atagtcgtga
     8641 gcttaacagt cttcttggtg gagctgtttt tgaggtttta ccaaatatcc aagcccagat
     8701 gaaacatccc gatgtgactc tcaaagtcga aattcgtgat gaagcagctt acatttctta
     8761 tgaagagatt aagggagcag gcgggttacc agtaggaacg tcagggaaag ggatgctgat
     8821 gttatcgggc ggtattgatt caccagtagc tgggtattta gcccttaaac gtggccttga
     8881 tattgaagtt gttcattttg ctagccctcc gtacaccagt ccaggtgcct tggctaaagc
     8941 ccaagacctt actcgtcggt taactcgttt tggtggaaat atccaattta tcgaggttcc
     9001 ttttacagaa attcaagaag aaattaaaaa taaggcacca gaggcttacc ttatgacatt
     9061 aacccgccgc tttatgatgc gcatcacaga tgccattcgt gagcaacgta agggtcttgt
     9121 tattgttaac ggtgagagcc taggtcaggt tgctagtcaa acccttgaaa gcatgcaggc
     9181 cattaatgcg gtaacatcga ccccaattat tagaccagtc gtgacaatgg ataaacttga
     9241 aattatcgag atggctcagg cgattgatac ttttgacatt tctattcaat cgttcgaaga
     9301 ttgctgtact atttttgcac cagaccgtcc taaaaccaat cctaaactag gtaatgctga
     9361 aaaatatgaa gaacgctttg atattgatgg tcttgtccag cgtgctgtgt caggaattgt
     9421 tgtcacagaa atcacacctg aaatcgtcaa tgatgaggtt gaaaatctca ttgatgcttt
     9481 actttaaaag aaaagatcaa gtgtaaatct tttagtagtc agtaatgata ctattaaaaa
     9541 gcctctataa gggctttttt gctgcaatga aggaggaaaa ttttgaggca agatgttata
     9601 atagagaaaa aggagggtgt gtcttgatta aaactatgca atacaaaaaa acgatggcaa
     9661 cagttgttgc tcttatcgtc gcacttttgc tatttggctt gatttatgat ttacttgggg
     9721 ttcaaaaaaa tgagctagca gctcaaaaat ctgctcagcc aaaggtcaaa acagcacgtg
     9781 ttgtagctaa tggcgacatt ttaattcatg atattctcta catgagtgct agaaaagctg
     9841 acgataccta tgatttcact ccttattttg aatatgttaa ggactggatt agcggtgctg
     9901 acttagctat tggagattat gaagggacca ttagcccaga ttatccctta gcaggctatc
     9961 ccttattcaa tgctccagaa gagattgcag gtgctctgaa aaatactggc tatgatgtag
    10021 tcgatttagc ccataatcat atcttagatt cccaattaga cggtgccctt aacactaaaa
    10081 aggtttttca tcaattaggt atagacagta ttggcatcta tgacaaggat cgttcaaaag
    10141 aatccttctt aattaaaaat gtcaatggta tcaaaattgc aattttaggt tattcttatg
    10201 gttataacgg catggaggcc acacttagcc aagaagacta tgagaaacac atgtctgatt
    10261 tagatgaagc taagataaaa aaagaacttc agctagctga aaaaaaggct gacgtgacca
    10321 ttgtcatgcc acaaatggga acagaatatg ccttagaacc gacagcagag caaaaagaac
    10381 tttaccacaa aatgattgat tggggagctg acgtcgtcct aggaggccat ccgcatgtta
    10441 ttgaaccttc agagacagtt ataaaaggta ggcaaaagaa attcattatt tactctatgg
    10501 gaaattttat ttcaaatcag cggcttgaaa ccgtagatga tatctggact gagcgtggcc
    10561 tattgatgga ccttactttt gagaaaaaag ataacaaaac aaaaattaaa acagttgagg
    10621 cccatccaac catggtttta gccaaaggta aaggcattgt gggtaaagaa ggttttgaac
    10681 tatacaatta tcgaacaatg gttttagaag attttatcaa aggtggaaaa taccacgaca
    10741 agattgatga ggaaaccaaa gcaaaagtag cacttgctta tcaggaaatt aatgatttag
    10801 ttaacctgaa atggtaatgt ttggtggttt agcatctaac gataaaaaat agagattaaa
    10861 cttaattttt ccttgataaa aagcagttat ggtgctaaaa tagtaacgtt gactaaatgc
    10921 acatcctgtg caaccgcgcg aaaatcgtta tcaagtagac caagtctcac gattccaggc
    10981 gagtcttcac agagggaaac ctcaaacaaa aattttatag gaggtgcatc atgagcacat
    11041 acgcaatcat caaaactggt ggaaaacaag ttaaagttga agtaggtcaa gcaatctacg
    11101 ttgagaaaat tgacgctgaa gctggcgcag aagtcacttt taacgaagtt gttcttgtag
    11161 gtggtgacaa aactgtagtt ggtactccag ttgttgaagg agctactgtc gttggaactg
    11221 ttgaaaaaca aggaaaacaa aagaaagttg taacattcaa atacaaacct aaaaaaggta
    11281 gccaccgtaa acaaggtcat cgtcaacctt acactaaagt tgtcatcaac gcaatcaacg
    11341 cttaatttac tagcttatga ttaaagcaat ttttactcgc caaaaaaatg gtcaactgag
    11401 cagtgttaca ctgacaggtc atgctggtag tggtaaacat ggctttgata ttgtttgtgc
    11461 ctctgttagt actctagcca ttaactttgt taattctctt gaggtgttag cagattgcca
    11521 agcattagta gatcttaatg atgttgaagg tgggtatatg gctattacaa taccaccaca
    11581 tgacaacaag gaggaagttc aattattatt tgaatctttt cttctaggaa tgactagttt
    11641 ggcgaaggat tcttcgaaat ttgtcaacac tcaggtcatc taaatttaat ttaagagagg
    11701 aaacaacatt atgttaaaaa tgaatcttgc taacttgcaa cttttcgccc acaaaaaagg
    11761 tggaggttct acatcaaacg gacgtgactc acaagctaaa cgccttggtg ctaaagctgc
    11821 tgacggtcaa actgtttcag gtggatcaat cctttaccgt caacgtggaa ctcatatcta
    11881 cccaggtgta aacgtaggcc gtggtggaga tgacacactt tttgctaaag ttgaaggtgt
    11941 tgtacgtttc gaacgtaaag gacgtgataa aaaacaagtt tctgtttacc cagtagctaa
    12001 ataattgttt aacatcgcat tatataaggc tttccaggtg tctggaaagc cgattttttt
    12061 attttggtac tcatgttgct acccatattt taaaaatcaa tgtaggaaga gagcttgtca
    12121 gcagttttat cttttgagga tttagtaaca tgagcgtaga tgttcatggt agtttggata
    12181 gtttcatgac caagtgtatt ggattttcat tgtttgtttc tatcctacta aagattgtag
    12241 ttgcttattt agagcaatag ttctaattgt aaaaagtcag cataataaaa caatgtcata
    12301 agatgttgtt ataacgggct ttatggtata attaaggata ctcattttag gaaggaaagc
    12361 aatgaatatt caacaattac gctatgtggt agccatcgct aataatggaa cttttcgaga
    12421 agctgcctct aaattgtttg tcagtcagcc tagcttatca gtttctatca aagatttaga
    12481 ggcggaatta gggtttcaaa tttttaatcg aactacttca ggaacagtct tgacaagtca
    12541 aggactagtt ttttatgaga aagctttaga agtagtcaaa tcttttgatt cttttgaaaa
    12601 aaccttttct caagctgatc tggatcaaaa tgaattttct attgctagcc aacattatga
    12661 ctttttgcca cctcttatta cagctttttc gcaacaatac gatggtcatc gtgtctttcg
    12721 tatctttgaa tcaacaacta ttcaaatttt agatgaggtt gctcaaggaa acagtgaaat
    12781 tggtattatt tacttgaatg tagacaatca aaaaggcctc tttcagcgga tggataagct
    12841 gggcttggaa tatgtttctc taattccttt tactactcat atttatcttt caaaaacgca
    12901 tccattggct aatcgcgaag cgctttattt gagcgatatt caaggattac ctgcggtaag
    12961 gtttacccaa gaaagggacg agtatttgta ttattctgaa aattttgtgg atactagtga
    13021 atgccctcgt atttataatg tgtcagatag ggcaaccttg aacggtattt tagagagaac
    13081 caatgccttt gcgacaggat caggttttct cgatcatcgg agtgttaatg ggattaaagt
    13141 gatacctcta gctgatcata ttgacaatca aatgatttat gtcaaacgaa aagataaaaa
    13201 cctttctgtt gctggggcca cttttgtgac tattttaaaa gactattttg ataaaagaag
    13261 gaaggcctta acatgaaaaa acgattgttt gtgcttagct tgatcctcct tgtagctttg
    13321 gatcaactta gtaaattttg gattgtttct catatagcgc ttggagaagt gaaacccttt
    13381 atcccaggta tcgtcagctt gacttacttg caaaacaatg gggctgcctt ttccatattg
    13441 caggaccagc aatggttctt tgttgtcata acggttttag ttatcggtta tgctatttat
    13501 taccttgcta ctcatcccca tttaaatatc tggaaacaat tagctctctt gcttattatt
    13561 tctggtggaa tcgggaattt tattgatcgt ttgcgtttag cttacgtgat tgatatggtt
    13621 catttagact ttgtggattt tgccattttt aatgtggcag attcatacct taccgttggt
    13681 gtcatattat tagtgatatg tttatggaaa gaagaggatt atggaaatta atgtgataac
    13741 atcggggcaa cgcttggata aggcactagc tgatttaagt cccctgtcac gtggtcaagc
    13801 caatgaccaa attaagcaag gccttgtttt agttaatggt cagcaaaaaa aggctaaata
    13861 tacggttcaa gcaggagatg tgatttgttt tgaactccct aaagaagaag tcttagagta
    13921 ccaagctcag aatattccct tggacattat ctatgaagat gatactctcg ccattattaa
    13981 caagccacag ggaatggtag ttcacccttc ggcgggacac ccttcaggaa caatggttaa
    14041 tgccttgatg tatcatatca aggatttgtc ctcaatcaat ggtgtggttc gtccaggaat
    14101 tgttcatcgg attgataagg acacctcagg tctcttaatg gtagctaaaa cagatgcagc
    14161 tcatcaagcc ttggcagaag aattaaaggc taaaaaatct ctcagaaaat acctcgccat
    14221 tgtccatgga aacctaccga atgatcgcgg gatgattgaa gctcctatag gtcgtagcga
    14281 aaaggaccgt aaaaaacagg ctgttacagc aaaaggcaaa gaagcagtga cacgttttac
    14341 agtgctagaa cgttttggcg attacagtct agtggaatta caactggaaa ctggtcgaac
    14401 gcaccaaatt cgtgttcata tggcctatat tggtcatcct gttgctgggg accctttata
    14461 tggaccacgt aagacactat caggttatgg ccaatttctt catgcaaaaa cattgggatt
    14521 aacgcatcct atgacaggaa aagaaatgat atttactgtt gaagctcccg aaatttttca
    14581 aaaggtgtta aagctgctaa gaaaaaaata aacagcgagg agtagccatt atttttagtc
    14641 ttatatcttt gtgtaaagtt tttaacatta taaaaaatac taaaaagcat ttacgataag
    14701 ccaggaaata tgagaagtct tttatgttat tgggatatgc aacatatctg ttagagatct
    14761 ttatttaaac tcttaaagaa aagagatatt ccaaatagat gctaggtatt gtctttttta
    14821 cttgtttttc ttttcttttt ttgctatact gattgaaata aaatattcct ttaaaacagt
    14881 cctgtgaggc tgtcaaggag acggattaaa aacatgcact atgatactta attgtgctat
    14941 attttgctga atctccttgt ttccaaggag atctttttat ttgaattttt agaggaggtt
    15001 tcaacatgaa aactaaggaa attgttgatg acgtgaccat gaaacgcgcc attacccgta
    15061 ttacttatga aattattgaa cgtaataaac aattagacaa tgtggttttg gcaggcatca
    15121 aaacgagagg tgtttttcta gctcgtcgca ttcaagaaag actgcatcaa ttagaagggt
    15181 tggatttacc aattggtgag ttagacatca agccttttag agatgacatg agagttgagg
    15241 aagacacaac tctgatgtca gtagatatta caggcaagga tgtgattttg attgatgatg
    15301 tgctttatac tggtcgtacc atccgcgcgg ccatcgataa cttagtgagt ttaggtcgac
    15361 cagcccgtgt tagtctagct gttttggtgg atcgtggcca tcgggaatta ccgattcgcg
    15421 cggattacgt tgggaaaaat attccaacaa gcagtgtaga agaaattgtt gttgaggttg
    15481 tcgaagttga tggccgtgat cgtgttagca ttattgaccc aacataagaa ggaggtttta
    15541 ctgtgaaaga tatcatttat gatgtggaag aagtgccaaa ggcaggtatg ttagttggtt
    15601 tgtcctttca gcatttattt gccatgtttg gggcgacggt tcttgttcct attttagttg
    15661 gaattgaccc gtctgtggcc ctcctatcga gtggtttagg aacacttgca catttatcgg
    15721 taaccaaatt taaaattcca gcctacatgg gctcaagttt tgcttatata gcagctatgc
    15781 agttactgat gaagacaaat ggtattggtg ctgtagctca aggggctatg acaggaggtc
    15841 tcgtttacct gattgtagct ttgattgtta aagctattgg caatgattgg attgataaca
    15901 tcttaccacc gattgtggtt ggtccgattg ttatggtcat tggtctaagc ctagcttcta
    15961 cagctgttaa tgatgtcatg ctaaaaaatg ggaactataa tctgacttat cttgttattg
    16021 ggttagtcac cttattatca gttatttttt tcaatattta tggtaaggga attgttgcta
    16081 ttgtaccgct tttattaggg ctattagttg gttatgttgt agccctttta gtgggtgttc
    16141 tcaccggtca agaaattgtt gattttacca atgtggctca ggccaaatgg tttagtatcc
    16201 catcagtgga aattcctttc ttaacctacg gggttaaatt ctacccaagt gccattttaa
    16261 ctatggcacc cattgccttt gttacaatga cagaacattt tggacacatt atggtcttaa
    16321 atagtttgac aaaaagagat tatttcaagg atccaggact tgaaaagact ttaactggtg
    16381 atggttttgc gcaaatcatt gccggcttct taggggcacc tccggtcact tcttatggtg
    16441 aaaacatcgg tgtaatggct ttgaataaaa tattctcggt ttatgttatt gcaggtgccg
    16501 cagtgattgc cgctctcctc agttttatcg gtaaggtatc agccttaatt caatctattc
    16561 caacaccagt tattggaggt atatcagttg ccttgtttgg tgtaatagct tctagcggtt
    16621 tgaaaatctt gattgaatcc aaagttgata tggataataa gaaaaactta ttgattgcca
    16681 gtgttatttt ggtctctggg attggaggat tgatgcttca agtaaatggt cttcaaattt
    16741 caggtgtcgc tttctcaaca cttcttggca tcatcttata tcaggtactt cctgaaaaat
    16801 aggaagaatt gttgttagac cacttacata gtcatcagtc gttagtgata aaggggataa
    16861 tcatgtcagt tgtaaataat cgcgttgcac ttaccaattt agtttcaatg gaagcattaa
    16921 ccaccgaaga agttttaggt cttatcaacc gtggttcaga atataaggct ggtaaggttg
    16981 ttatttcgga tcatcaaaag gatcttgttg ccaatttatt ctttgaaaac tccacccgaa
    17041 cccataagtc ttttgaagtg gctgagaaga aactcggctt gacagttctt gatttcaatg
    17101 ctgatgcaag tgcggttaac aagggagaaa gtttgtatga tactgtgtta accatgagtg
    17161 cacttggaac agacatctgt gtgattcgtc acccagaaga tgattattac aaggaattag
    17221 tagaaagtcc aactattaca gcttccattg tcaatggagg agatggttct ggacaacacc
    17281 cgagtcaatg tttacttgac ttattaacca tctatgaaga atttggacgc tttgaaggct
    17341 taaagattgc gatcgctggt gacttaactc actctagggt ggcaaagtcc aacatgcaaa
    17401 tcctcaaaag attaggagca gagctttatt tctatggacc tgaagaatgg tattcagaag
    17461 cctttaatgc ttacggcact tatattgcta ttgaccagat tattaaggaa ttggatgttc
    17521 ttatgttgct gcgtgtacag cacgaacgtc atgacggcca tcagagtttt tcaaaagaag
    17581 gttaccatca agcgtttgga ttgacacagg aacgttacca acaattaaaa gattctgcta
    17641 ttattatgca tccagctcca gttaatcggg atgtggaaat tgcagatagt ttggtggaag
    17701 ctcctaaagc gagaattgta tctcagatgg ctaatggtgt ctttgttcgc atggctatta
    17761 ttgaagccat tttgaatggt agaaataaaa actcatgatg aaagattaaa gcatgtttag
    17821 aaggtaggaa aaggtaggaa aatgacaaaa cgattactta ttttagaaga cggcactatt
    17881 tttgaaggag aaccctttgg tgctgatatt gatgtgactg gtgaaattgt ctttaataca
    17941 ggaatgacag gctaccaaga atccatcact gaccaatcct acaatgggca aattttaacc
    18001 tttacctatc ctttgattgg aaattatggg attaatcgag atgactatga gtccatttca
    18061 cccacctgta aaggggttgt tgtgtctgaa gtcagtcggt tggcaagtaa ttggcgcaag
    18121 caaatgacgc tagatgcgtt cttgaaaata aaagggattc cagggatttc gggaattgat
    18181 acgagagctt tgaccaaaat tattcggcaa cacggtacca tgaaagctac gatggcagat
    18241 gatggagata gtatccagca tttaaaagat caactgcgtg caacagtctt accgactaac
    18301 accattgaac aagtgtcaac taaaacagct tatcctgcac cagggatcgg gaaaaatatt
    18361 gtattggttg attttggttt aaaacattct attttaagag aattttctaa aagacaatgt
    18421 aatatcaccg ttgtcccttt caacatcact gcagaagaag tgctccaact aaatccggat
    18481 ggcttgatgc tatctaatgg cccaggaaac ccagaagatt tgccagaggc tttagacatg
    18541 attcgtgggg tacaaggtaa gattcctatt tttggtattt gtatgggaca tcaactcttt
    18601 agtctagcaa atggtgccaa gacttgtaaa atgacctttg gtcatcgtgg ctttaaccat
    18661 gccgttagag aaattgcaac gggaagaatt gattttacca gtcaaaacca tggttatgcc
    18721 gttgaacgta gtagtctacc tgatacttta atggttacgc atgaagacat taacgataag
    18781 acggtcgaag gagtgaaaca tagagatttt ccagcttttt cagtccaatt tcaccctgat
    18841 gcggctccag gacctcatga tgccagctac ttatttgacg aatttttgga aatgattgat
    18901 tcttggagat gtacgagtaa atagtaaatc agttgcaatt tcacgactat atcacctctt
    18961 gttactctat ttattaacat ttacagttga tttagtaccc tatcaaaaat attatttaat
    19021 gttgctctgt gaggcaacaa atagagaaag gaaggcaagg agaacacaag taaataattt
    19081 agtaaaaagt taggttttca aagagataaa gctcttattg agaagtgctg actttaccgc
    19141 ttcttgtggt attcttggta tcattaatta tgccaaaacg aaaagatatt caaaaaatca
    19201 tggttatcgg gtctggcccg attattattg gacaagctgc tgaatttgat tatgcaggga
    19261 ctcaggcctg tttggctcta aaagaagaag gctataaggt catacttgtt aattcaaatc
    19321 ctgcaacgat tatgacagat aaagaaatcg ctgataaggt atatattgag cctttgactc
    19381 ttgaatttgt taatcgtatc attcgaaaag aaaggccaga tgccatactg ccaaccttag
    19441 gtggtcaaac gggcctcaat atggcaatgg cattatctaa agctggtatt cttgatgatt
    19501 tagagattga attgttaggt actaagttat ctgcgattga tcaggcagaa gacagagacc
    19561 ttttcaaaca attaatgcaa gaactagacc aacccattcc agaatctaca attgttaaaa
    19621 cagttgatga agctgttaca tttgctagag atatcggtta tccagtcatt gtgcgcccag
    19681 cttttactct tggtggaact ggtggaggaa tatgtagtag tgaagaagaa ctttgtgaaa
    19741 ttactgaaaa cggcctaaag ttatcaccgg tcacacagtg tttgattgaa cgctcaattg
    19801 ctggcttcaa agaaattgaa tacgaagtaa tgcgagatag tgctgataac gcgctggttg
    19861 tttgtaatat ggaaaacttt gatcccgtag ggattcacac aggagattct attgtatttg
    19921 caccaacaca gaccctttct gatattgaaa atcaaatgtt acgtgatgct agtttaaaaa
    19981 taattcgagc tctgaaaatt gaaggtggct gtaacgtcca gttagcacta gatccttata
    20041 gtttcaaata ttatgttatt gaagtaaatc ctagagtgtc tcgttcctct gcccttgctt
    20101 caaaagcaac aggttatccc attgccaaat tggctgcaaa gattgctgtt ggcctaacac
    20161 ttgatgaaat gattaaccct atcacaggca ctacttacgc tatgtttgaa cctgcccttg
    20221 attacgttgt tgctaaaatt ccgcgttttc cgtttgataa atttgaacat ggtgaacgcc
    20281 aactgggaac tcaaatgaaa gcgacaggtg aagtcatggc tattggccgt aatttggaag
    20341 agtccttact aaaagcctgt cgttctttag aaattggtgt ttgccataat gagatgacaa
    20401 gtttaagtaa cattagcgat gaagagttag tgactaaagt gattaaagct caagatgatc
    20461 gactttttta cttgtcagaa gcaatacgac gaggttatag tatcgaagaa cttgagagct
    20521 tgaccaagat tgatttattt ttcttggata aattacttca tattgttgaa atagagcaag
    20581 aattacaaat gcatgttgac catttggaaa gcctcaaaaa agcaaagcgt tatggatttt
    20641 cagatcaaaa aattgcagaa atttggcaaa aggatgaaag tgatatcaga gcgatgagac
    20701 attctcatag cctgtatcct gtctataaaa tggtagatac ttgtgcagct gaatttgatg
    20761 ctaaaacccc ttatttttat tcaacatatg agttggaaaa tgaatctgtt cagtcgaata
    20821 aagaatcaat tttagtttta ggttcaggcc ctattcgtat tggtcaaggg gttgaatttg
    20881 actacgcaac ggttcattct gttaaagcca tccaaaaagc tggctatgaa gccattatca
    20941 tgaattcaaa tccggagact gtatctacag atttttcggt atcagataaa ttatattttg
    21001 aaccgttaac ctttgaagac gtcatgaatg tgatagattt agagcagcct aagggagtaa
    21061 tagttcagtt tggaggacaa actgctatta acttagcaca agcactttct gaggcgggtg
    21121 ttacaatatt aggaacacaa gtagaggatt tggatagggc tgaagacaga gatttatttg
    21181 aaaaggcgtt gaaagagctt ggaatcccgc aaccacaagg tcaaacggca actaatgaag
    21241 aagaagcttt agaggcagct aaaaaaattg gttttccagt cttagttaga ccttcttacg
    21301 tacttggtgg ccgggctatg gaaattgttg aaaacaaaga agatcttaga gaatatataa
    21361 gaacagcggt taaagcaagt cctgaacatc ctatcttagt ggactcttat attttcggta
    21421 aggagtgtga agtggatgcc atttcagatg gaaaatcagt cttaattcct ggtattatgg
    21481 agcacatcga aagagcaggt gttcattcag gagattcaat ggcagtttat ccgccacagc
    21541 aattatctaa gcaaattcaa gaaacaatag ctgagtatac taaacgttta accattgggt
    21601 taaattgtat tggtatgatg aatgttcaat ttgttattaa aaatgaacaa gtctatgtta
    21661 ttgaagtgaa tccacgcgct agtcgtaccg tgcctttctt atcaaaagta acaggtattc
    21721 cgatggctca gattgctact aagttgattt taggtcaaac attaaaggat ctaggatatg
    21781 aagatggtct ttatccccaa agtcaactgg tgcatatcaa agcgcctgtg ttttcattta
    21841 caaagttggc acaggttgat agtcttttag gtcctgaaat gaaatcaact ggagaagtaa
    21901 tgggttcgga tactagtctt gaaaaagcac tttataaagc gtttgaagcc aataattctc
    21961 atttatcaga atttggtcaa attgtcttta ctatagcaga tgatagtaag gcagaagctc
    22021 taagtctagc aagacgattt aaggcgattg gctatcaaat tatggctact caaggtacag
    22081 cagcttactt tgctgagcaa ggcttaagcg cgtgtttagt tggaaaaatt ggtgatgctg
    22141 ccaatgacat cccgacactc gtgcgacatg gtcacgttca ggcaattgtt aatacagttg
    22201 gaattaaacg cacagctgat aaagacggac agatgattag aagttcagct attgaacaag
    22261 gagttccttt atttacagct ttagatactg caaaagctat gttaacggtt ttagaaagca
    22321 gatgttttaa catcgaagcg atataaaaat gattttttta gattataatg acaaaagttt
    22381 cagtaagcta ttaatttgtc atagcatata agtcattatc ttgtggcttg ttttcgacta
    22441 agggctaaga atttcttgac aacaatataa aaaggtcata tacttgtctt atacaaatag
    22501 tacaaaaggg gaggatagaa atgtctaaaa gaggaaaaat taaaataaca acgaaaacaa
    22561 agcttattac agctagtgtt ataacgctag tattaattat aactggagta gtcttgtgga
    22621 aacaacaaca aaatacgcta acagctgata tcgctaaaga accttactct actgttagtg
    22681 taactgaagg gagtattgct tcttcgactt tactatcagg tactgtaaag gctttatcag
    22741 aggaatatat ttattttgat gctaataaag gaaatgatgc aactgttaca gttaaagtag
    22801 gtgatcaggt aacgcagggc cagcaattag ttcaatataa tacaacaaca gctcagtcag
    22861 cttatgatac tgctgttagg agtcttaaca agattggccg acaaattaat catcttaaaa
    22921 catacggagt tcctgctgtt agtacagaaa ctaataaaga tgaagctacc ggtgaagaga
    22981 cgacaacaac agttcaacca tcagctcagc aaaatgctaa ttataaacag cagctgcaag
    23041 atttaaatga tgcttatgca gatgcacaag cagaagtaaa taaagcgcag atagcgttaa
    23101 atgatacagt agttatcagt agtgtctctg gaactgttgt ggaagtaaat aatgatattg
    23161 atccttcttc aaagaacagt caaacacttg ttcacgtagc aaccgaagga cagcttcagg
    23221 tgaaaggaac attgacagag tatgatttag caaacgttaa ggttggtcaa tctgtaaaaa
    23281 ttaagtctaa agtttattct aatcaagaat ggactggaaa aatatcatat gtttcaaact
    23341 atccaactga gtctaatgca ggttcaacaa cgccagcagg tagcactgga gtgggaagtt
    23401 ctacaggagc tgcctatgat tacaagattg atattataag tcctcttaac cagcttaaac
    23461 aaggtttcac tgtttctgtt gaggttgtta atgaagctaa acaggcctta gttcctttaa
    23521 cggctgttat taagaaagat aaaaaacact atgtttggac ttatgatgat gctactggca
    23581 aagccaaaaa agtagaggtg acacttggaa acgcagatgc acaacaacaa gaaattcata
    23641 aaggagtaac tgttggtgac attgttattg ccaatccaga taaaaatatc aaaccggata
    23701 aaaaactaga aggggttatt tcaataggta ccaacacaaa accggaaaaa gattctcaat
    23761 caaagaataa aaaatcaggg gtggataaat agtgtcgcat gaaaaaaaac aattaatgca
    23821 attatcaaat attgtgaaga gctatcaaaa tggggatcaa gttcttaaag ttttgaaagg
    23881 tattaattta acagtatatg agggtgaatt tttagctatt atgggtccgt ctggttcggg
    23941 aaaatcaaca ctaatgaata ttattggttt attagatcgt ccaacatccg gagattacac
    24001 attacataat actaaaatag aaatccttaa tgatcgtgaa ttagcaaaag tacgaaatga
    24061 tgaaatcggc tttgtctttc aacaattctt tctgttagct aaattaactg cattacaaaa
    24121 tgttgagttg cctcttattt atgctggtgt aaacgtctca aaaagacgtg aacaggctaa
    24181 gcaatttctt gaaaaagtag gacttggacg acgtatcaaa catctaccgt cagaactgtc
    24241 aggaggtcaa aagcaaaggg tggctatcgc acgagcttta gtaaatgacc catctattat
    24301 tttagcggat gaaccaacag gtgcactaga tactaaaaca ggtcagcaaa ttatggaact
    24361 cttgacagag ttaaacaaag aaggtaaaac aattattatg gttactcatg aaccagaaat
    24421 tgctgatttt gcaactcgga aaattattat tcgtgatggt gatatcacaa cggatactac
    24481 agctagtgtt gttattgact aggaggaaaa ataatggaaa attggaaatt cgctcttagc
    24541 tcaatttggg ggcataagat gcgctcaatc ctgacaatgt tgggaattat catcggtgta
    24601 gcagcagttg tgattattat ggggttggga aatgctatga aaaatagtgt aacaagcaca
    24661 ttttctagca agcaaaaaga tattcaactc tattttcaag aaaaaggaga agaagaagac
    24721 ctgtatgccg gattgcacac gcatgaaaat aatcacgaag tgaagccaga gtggttagaa
    24781 caaattgtta aagatatcga tggtattgat tcttattact ttaccaattc tgctacttct
    24841 actatttcgt atgaaaaaaa gaaagtagac aatgcttcga tcataggggt aagtaaagat
    24901 tattttaata taaaaaacta tgatatcgta gctggtcgta cactaacaga taatgattat
    24961 tctaattttt cgcgtatcat tcttttagat acagtattag ccgatgacct atttggcaaa
    25021 ggtaattata agtcagcttt aaataaagtg gtaagtttat ctgataaaga ttatttagtt
    25081 attggtgttt ataaaactga tcagacgcca gtttcttttg atggattatc aggcggcgct
    25141 gtcatggcga atactcaggt agcttcagag tttggcacca aagaaatagg atctatctac
    25201 attcatgtga acgatattca aaacagtatg aacttaggaa atcaagcagc tgatatgcta
    25261 accaacatat cgcatattaa agatggacag tacgctgtcc ctgataatag caaaatcgtt
    25321 gaagaaatca atagtcagtt cagtatcatg acaacagtca taggttcaat tgcagcgatc
    25381 tctcttttag tgggaggaat tggtgttatg aatattatgt tggtatctgt cacagagcga
    25441 actagagaaa ttggtttacg taaagcactt ggtgcaacac gcctgaaaat tttatcacaa
    25501 tttttgattg aatccgttgt tctaacagtt cttggaggct tgataggatt attattagct
    25561 caattaagtg taggggcttt aggaaatgcg atgacattaa aaggagcttg tatttctctt
    25621 gatgtggcct taatagcagt tttattttct gcctcaatag gtgtcttttt tggaatgttg
    25681 ccagctaata aagctagtaa gttagatcca attgaggcat tacgttatga atagaatagt
    25741 tcgtatcaat taatctatag acgctgacta gtttaacgtc tgattatatc tagtttttgt
    25801 attagcacag tagttatatc attgaagtgt tagtgcattt tttgaggtga taatatctag
    25861 caaagctatc attggtatga tgatgtggat agcgtataag tgatacatct cttatttcta
    25921 gctgaacttc ggttcggcta ggaagttgtc aggcaatgag ttgtttaaaa taaaggagat
    25981 tagttcaatg acatttttat ctgatttgat atcattaatg acaaaaatca gattatcttg
    26041 ggtaataaag gcgggtattt ttcaattatt atttgtaacg attgctaata ttgtcttatc
    26101 agaatttttc tattttatat tagacgttac tggtcaatat catttagata aagacaatgt
    26161 tgtgactttt ttaaaaaatc ctatagcact tgctttatta ggtgcctatt tatttttatt
    26221 agctgctttt attcaccttg agttttttgc tctatatcga attattgcgg atcaagaaat
    26281 tagtttctat ctttttagaa aacagttttc ttattaccta agggggcttt ggaaaacatt
    26341 ttctggttac caattattac tttttttgct ttatatccta ctgactattc cagtcttaca
    26401 tattggttta tcttctgtga ttactcaaaa gctttatctt ccagaattta ttgttgggga
    26461 attatcaaag ataactagca caaagtactt gctttatggc agtcttattc ttgtgtttta
    26521 ccttaaccta agattagtat attttttacc attgatagca atcaaccatc gtacggttgc
    26581 tcaagcatgg agagagagtt ggcaaaagac taaaaagaaa catgtattgt tatggatgaa
    26641 actttttgca atcaatggtc ttacgcttgt agtcttatcg ctagctattt ccatgattct
    26701 tatttttgtt gatatgttta atcctaaggg gaataatatt attgttcagc tgggagcttt
    26761 gacctttaca tgggagctca ttttttttac tactattttt tttaaactct gttcagcaat
    26821 gattttaaaa gaggcaattg aaccacaaaa gcaatatgat gagccaagaa gaagtaataa
    26881 ggcatatgtt gtaatcttta tcgtggtgac agtaggtttt gcttatcaat ctcttgaacg
    26941 tttaactttt tttgacacat ctcactctaa gacagttatc gcgcatagag gacttgtatc
    27001 agcaggtgta gaaaattcac tggaagccct tgaaggtgct aagaaagcag gaagtgatta
    27061 tgtagaactg gatctaatct tgactaagga taatcacttt gtggtgtctc atgataatcg
    27121 attgaagcgt ttagctggag taaataagac gattcgcaac ttaaccttaa aagaagttga
    27181 acatctaacg agtcatcaag gacatttttc agggcgtttt gtttcttttg acacttttta
    27241 tcaaaaggct aagaagttga atatgccatt acttattgaa ctcaagccaa ttggtacaga
    27301 acctggaaat tatgtcgatt tgtttttaga aacttatcat cgacttggta taagcaaaga
    27361 taataaagtc atgtctttag atttagaagt aatagaagct atcaagaaaa aaaatccatc
    27421 aattacgact ggttatatca taccaattca atttggcttt tttggagatg aatttgttga
    27481 tttctatgtc attgaagact tttcttatcg gtcttatttg tcgtcccaag ctttttggaa
    27541 taataaagaa atttacgttt ggactattaa tgatcccaag cgcatagagc attatctctt
    27601 aaagcctatt cagggaatta ttacagatca accagcttta actaatcaat tgattaaaga
    27661 cttaaaacaa gataattctt attttagtcg attagtcaga attattagta gtctttatta
    27721 aaggtttaaa gaaagtgtca agaaaaaaac ttgacac
//
LOCUS       DATURV010000019        25253 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DATURV010000019 DATURV010000000
VERSION     DATURV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25253
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.019"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..888)
                     /locus_tag="VCF75_000776"
     CDS             complement(<1..888)
                     /locus_tag="VCF75_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP2546356.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPIMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLET"
     gene            complement(1033..1410)
                     /locus_tag="VCF75_000777"
     CDS             complement(1033..1410)
                     /locus_tag="VCF75_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP2546357.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            complement(1421..2086)
                     /locus_tag="VCF75_000778"
     CDS             complement(1421..2086)
                     /locus_tag="VCF75_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP2546358.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEDDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            complement(2135..3220)
                     /locus_tag="VCF75_000779"
     CDS             complement(2135..3220)
                     /locus_tag="VCF75_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP2546359.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLIKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            3394..4395
                     /gene="ccpA"
                     /locus_tag="VCF75_000780"
     CDS             3394..4395
                     /gene="ccpA"
                     /locus_tag="VCF75_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEP2546360.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            4526..5524
                     /locus_tag="VCF75_000781"
     CDS             4526..5524
                     /locus_tag="VCF75_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP2546361.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADPKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            5526..6860
                     /locus_tag="VCF75_000782"
     CDS             5526..6860
                     /locus_tag="VCF75_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP2546362.1"
                     /translation="MRVGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            7282..9225
                     /gene="thrS"
                     /locus_tag="VCF75_000783"
     CDS             7282..9225
                     /gene="thrS"
                     /locus_tag="VCF75_000783"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEP2546363.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSRPDAQA"
     gene            9365..10357
                     /locus_tag="VCF75_000784"
     CDS             9365..10357
                     /locus_tag="VCF75_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP2546364.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNDVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELKPGQEQVVSQFMGLPDIT
                     VERHELSLDIQYDSSRYQTADIIQKTMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            10359..11177
                     /locus_tag="VCF75_000785"
     CDS             10359..11177
                     /locus_tag="VCF75_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEP2546365.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLMVLSGIKVMAGLSILQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKVVSIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            11179..11964
                     /locus_tag="VCF75_000786"
     CDS             11179..11964
                     /locus_tag="VCF75_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546366.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            12165..12314
                     /locus_tag="VCF75_000787"
     CDS             12165..12314
                     /locus_tag="VCF75_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEP2546367.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            12709..13857
                     /locus_tag="VCF75_000788"
     CDS             12709..13857
                     /locus_tag="VCF75_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEP2546368.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNTVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            13814..15061
                     /locus_tag="VCF75_000789"
     CDS             13814..15061
                     /locus_tag="VCF75_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEP2546369.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDGELLKKADFAVL
                     SSGTTGKAKLFWRRLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPVGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            15117..16151
                     /locus_tag="VCF75_000790"
     CDS             15117..16151
                     /locus_tag="VCF75_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEP2546370.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            16313..17023
                     /locus_tag="VCF75_000791"
     CDS             16313..17023
                     /locus_tag="VCF75_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2546371.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            17016..18368
                     /gene="vicK"
                     /locus_tag="VCF75_000792"
     CDS             17016..18368
                     /gene="vicK"
                     /locus_tag="VCF75_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEP2546372.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWLEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            18372..19181
                     /locus_tag="VCF75_000793"
     CDS             18372..19181
                     /locus_tag="VCF75_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP2546373.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            19613..20305
                     /locus_tag="VCF75_000794"
     CDS             19613..20305
                     /locus_tag="VCF75_000794"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEP2546374.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVVIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            20306..23845
                     /gene="smc"
                     /locus_tag="VCF75_000795"
     CDS             20306..23845
                     /gene="smc"
                     /locus_tag="VCF75_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEP2546375.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHTAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKCVQELERDYQLNQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGTNRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKNALTCDIDDIKENKDLIR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAINQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIQKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(24098..24949)
                     /gene="rgg3"
                     /locus_tag="VCF75_000796"
     CDS             complement(24098..24949)
                     /gene="rgg3"
                     /locus_tag="VCF75_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEP2546376.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDKDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     SLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            25029..25100
                     /gene="shp3"
                     /locus_tag="VCF75_000797"
     CDS             25029..25100
                     /gene="shp3"
                     /locus_tag="VCF75_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEP2546377.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
BASE COUNT         7905 a         4398 c         5239 g         7711 t
ORIGIN      
        1 agtttcaagg aatatttttg aaatatattt tccaataata cccatgcaaa agagctgaat
       61 gcctcccatg aataaaataa tagagacagt gctagcccaa ccagagacag gatcaccaaa
      121 gagtatcttt ctgataatga taaataagat ggcgaaaata gaaatcaaaa agctgaaagt
      181 ccctgtccag gtagcaatgg ttaacggcat ttctgaaaag ttgataaagc catctaaaga
      241 atatcggagc aactcccaaa aatgccaacg actttttcca tatttgcgtt tttggttttc
      301 aaaactaaga taggtgatac gataaccaac ccacgaaaag atcccttttg aaaaacgatt
      361 aacctctccc aactcaagaa tgctatcaac gacttgtctg gtcattaacc gataatcccg
      421 aactccattg accatttcag tatctgataa gtgtttgatc agtccataaa atagattcga
      481 acacattgaa cgaattagtg gttctccttg tctattttgg cgcctggtgc caacgatgtc
      541 ataaccttct tttagcttag cgtacataat tggcaaaagt tcgggtggat cttgcagatc
      601 cacatccatc acagtaatgt aattgccttt ggcttctttt agtccagcta agagcccagc
      661 ttccttgcca aaatggcgtg aaaaagacag ataatgcaca tttggaaagc gagcagctag
      721 ctcacgcaaa atacccaagg tgttatcctt agaaccatca tcaataaaaa tgtattcaaa
      781 agcaagttgg ttggtcatgg acgtttctag ttgatgcatt tcttcaaaat aagggagaat
      841 gtttgcttct tcattaaagc aggggacaat gatagatagt agtgtcatag acttttcttt
      901 caaatggaat tttagggcaa ttgtaacaca aaagctgaga tatttcaaaa ctctgttaat
      961 aatgagattg tcccaatcgt caggttaaaa aataaaacaa cagaagcttt aaagcttctg
     1021 ttgttaatag tattagttgt ttaaatcaat gacttcaatt ttgtagccat ctggatcctg
     1081 aatgaaataa tagcgggcag atttgtcagc cagttctttg atatcggtaa ctggaaaacc
     1141 tgcttgtcga tgttttttat ggtctgcttc aaaatgttca gagccaaggg caatatgacc
     1201 atagccattt cctaagtcgt agtcgccatg accataattg taggttaatt ctaattcata
     1261 actttctcct tctaaagcca gataaacaag agtaaattga ctatctggaa aatctttacg
     1321 atagttttcc ttaaaaggga aggcgcttgt gtaaaaagct accgactggt caaggtcttt
     1381 gacgcggata caggtatgta atgctttcat tttaatctcc ttatttaacg actgaaatca
     1441 cttcttcttt aggcttacga acttgagcat gtttgggatc tcgcaggcga tagcctaggg
     1501 ataacatgct agcaatgcct tctttttcta aatcaatcac attatgctta gctaggatat
     1561 gattgacctt atcataatga aagccttcaa tagggcaagt atcaatgccc aaaagagcag
     1621 ctgtcatcat catattacca agagcgatat aggtttgttt agctgtccaa tcaaagagtg
     1681 cccgaggatt atctgccata tccatgtcct ctttttggaa agattcatag agttttaggc
     1741 ggctgttgag accatcatct tctttgatac cacgtcgtaa aaggctattt ttgatagcag
     1801 ggctatcgta tcttgcatgt ttttcagcta ttaaaagaat aaagtgacta gctgtctcta
     1861 gttgatactg ggccccccag gcaaagggct tgatttcttc tttaataggc ttgttgtcta
     1921 aaacgacaaa gcgccagcct tctaagccaa cagaagaagg gcttaaccaa gcagcatcaa
     1981 ggataagggc taaatcctca tcagaaatct tttcttcctt ataaacacga acggctgttc
     2041 taaagtgtag tgcttgctgt atttgatgat gaatggtttg atccatacga ataccttcct
     2101 ttcgcgaggg tttgtttaac cagatggtag gggcttagcc ttcaaaataa agcaactctt
     2161 ttggagtatg ggtaaagact tcaaagccat ttttagtgac atggccacag tcttcgatgc
     2221 gcacaccgac ctttcctgga atgtaaatac caggttcaac agagaagcac ataccttctt
     2281 caaggaccaa gtcattgcca gccataatgg agggaaattc gtggacatcc atgccgatac
     2341 catgtcccaa gcggtggttg aagtactcac catatccagc cttttcaatc acttgacgag
     2401 cagcagcatc tacttgagca gctgttacac ctggtttgat aaagtcaata gccgctagtt
     2461 gtgcctctag gcagagattg tagatatcga ttttaaattg gtctggctga ccaacagcca
     2521 cagttctggt catatcactg gtgtagccta aggtctcaac acctaagtca aaaagaagta
     2581 gggcattgtt ttcaatatta tttgttccag gaatgccatg gggattggct gcattatttc
     2641 cagttaagac catagtgtca aagctcattt tatgaatgcc ttgttttttc atttcaaact
     2701 caatttgagc gatgacgtct gtttcggtag catctaagga aatattgtca aaaccaactt
     2761 gaacagcctt atcagcgaac tgcccggcaa tcatcatttt attgatttca tcagctgatt
     2821 tgatgaggcg catgccttgg acataagggg ttagattgtt aaattgccct gaaaagatag
     2881 tctgcaagcc atggaactta ttgacgttaa ggtggtcaaa ttcagcataa atggttttgg
     2941 ctgctgtatt tggtaaaacg gccttgatct tttcccaagg attttcagaa tcaacataac
     3001 caaacacagg aaatgatatg gcctggctag ctctggccac ctcaagggca ggtacaaaaa
     3061 gcacaggagc taaatcatgg tagacaaaca agaacagttg tctttcatgt gggtcacaga
     3121 agaatccagt taaatagtta atcgtaacag gatctgagaa aatcgccaat tcagctccct
     3181 tttggtcaag atacaaacgt atttggtcta attttgtcat aggctaacct tctttctact
     3241 tactattttt tcaaaaaaag cttaaaaaat caagcttcga gttaattttt gaaaactttt
     3301 tcaaaaatta acctataaca cttgaaagtg gttacaaatc atgctaaaat actttttgaa
     3361 agcgtcattt tcaaatagaa aggaagataa aaaatgaata cagatgatac cattacaatt
     3421 tatgatgttg cccgtgaagc tggtgtttca atggcaaccg ttagtcgtgt tgttaatggc
     3481 aataaaaatg ttaaggaaaa tacacgtaag aaagttttag aagttattga tcgccttgac
     3541 tatcgtccca atgctgtcgc gcgtggtctc gccagcaaaa agacaacaac cgttggcgtt
     3601 gtgattccaa atattgcaaa tagttatttt tctatcttag ctaagggtat tgacgatatc
     3661 gccgctatgt acaaatataa tattgtgctt gcctccagtg atgaagatga tgacaaggag
     3721 gttaatgttg tcaataccct ttttgccaag caagtggatg gtattatttt tatggggcat
     3781 cacttgacag aaaaaatccg ggccgaattt tcacgctctc gtactccagt tgttttggca
     3841 ggaactgtag atcttgatca tcaattacca agcgtcaaca ttgactatag agctgcggtg
     3901 tcaaacgttg ttgatatttt agctgaaaat cataagtgta tcgcttttgt gtcaggacca
     3961 ctcattgatg atatcaatgg taaagtgcgc ttagcagggt acaaagaagg gttgaagcac
     4021 aataagcttg acttcaaaga aggtcttgtt tttgaagcga actattctta taaagaagga
     4081 tttgaattgg cgcagcgcgt cattaactca ggagcaacag cggcttatgt ggctgaagat
     4141 gaattggcag caggcctttt aaacggcttg tttgaagcag gtaaacgcgt accagaggac
     4201 tttgaaatca tcactagcaa cgattcacca gtggttcaat acactcgtcc caatttgagt
     4261 tctatcagtc aacctgttta tgacttaggt gctgttagca tgcggatgtt gactaaaatc
     4321 atgaacaaag aagagttgga agaaaaagaa attcttttga atcatggtat taaaaaacgt
     4381 gggacaacta agtaactgtg acttatgagc accatctaac aaaaagtatc acaatagact
     4441 gtttcttcaa tactaaaatc agccctaagg ctgatttttg catgtcttag tgaaaaatac
     4501 tataaaatga gctataatag aacttatgaa agtcttattg tatttagaag cagaaaatta
     4561 tctaagaaaa tcaggaattg gtcgagcgat taagcatcag gctaaagcct tgtcacttgt
     4621 tggtcaacat tttacgacta atccaagaga aacttatgat ttggttcatc tcaataccta
     4681 tggtttaaaa agttggctgc tgatgataaa agcacaaaaa gctggtaaga aggttatcat
     4741 gcatgggcat tctacagaag aagattttag aaattctttt attttttcaa atctattatc
     4801 tccttggttt aaaaaatacc tttgtcactt ttacaataag gcagatgcta tcattacccc
     4861 taccctatat tctaagtctt tgattgagag ttatggagtg aagtcaccta tttttgcagt
     4921 gtcaaatggg attgatttgg agcagtacgg agcagatcct aaaaaggaag cagcttttcg
     4981 tcgctacttt gacattaaag agggtgaaaa agtggttatg ggagcaggat tattttttct
     5041 gaggaaagga attgatgact ttgtcaaagt tgcccaagct atgccagatg ttcgttttat
     5101 ctggtttggc gagaccaaca aatgggtcat tcctgctcaa gttcgccaaa tggtcaatgg
     5161 taaccacccg aaaaatctta ttttcccagg atacattaaa ggggatgttt atgaaggtgc
     5221 catgactggt gcagatgcct ttttctttcc aagtcgtgaa gaaacagaag gcattgttgt
     5281 cttagaagcc ttggccagtc gccagcacct tgttttacgt gatataccag tttactacgg
     5341 atgggttgat caaagtagtg cggaattagc aaccgatata ccaggtttta tagaagctct
     5401 gaaaaaagtc ttttctggtg ccagcaacaa agttgaagct ggttacaagg ttgcccagag
     5461 tcgtcgccta gaaacggttg gccatgcctt agtagatgtc tataaaaaag taatggagtt
     5521 ataagatgcg tgtaggtcta ttcacagata cctattttcc acaagtttca ggagtcgcta
     5581 ctagtattcg tacgttaaaa gaagagctag aaaaagaagg tcacgaagtt tatattttca
     5641 ccactactga tagagatgtc aaacgctttg aagacccgac cattattcga ctgccaagtg
     5701 ttccttttgt gtcatttacg gatagacgtg tggtttatcg tggcctcatt tcgtcataca
     5761 aaattgcaaa acactataat cttgatatta ttcatacgca aactgagttt agcttaggtt
     5821 tattagggaa aatgataggc aaagctttgc gaattcctgt tgtccatact taccataccc
     5881 aatatgagga ctacgtgagt tatattgcca acggaaaaat cattcgacca agtatggtca
     5941 aacctcttct taggggctat ttgaaggatt tggatggggt tatctgccca agtaggattg
     6001 tcctcaatct tctagaaggt tacgaagtga ctatccctaa gcgggttatc ccaacaggca
     6061 ttcctttgga aaaatatatt cgtgatgaca tcacagcaga agaagtaacc aacttaaaag
     6121 cagaattggg cattgctggt gatgaaacca tgttattgag tttgtcacgg atttcttatg
     6181 aaaaaaatat tcaagctatc atcaatcaga tgccagctat tttggctgaa aatgccaaga
     6241 taaagcttat tattgtagga gatggcccct atttgcaaga tttgaaacac ttggcgatgc
     6301 agttagaggt tgacaaacac gtgaccttta caggcatggt gcctcatgat aaggttgctc
     6361 tgtactataa ggcttgtgat ttctttatct cagcatcgac cagtgagact cagggcttga
     6421 cctatattga aagtttggct agtggcactc ctattattgc tcatggcaat ccttatttag
     6481 atgatgtggt gactgataaa atgtttggca ctctttatta cgctgaaaca gatttaactg
     6541 atgctattat tgatgccata ctaaaaacac cagttatgga taaacggtta ttagcaaaaa
     6601 aacgttatga aatctcagca cagcactttg gaaaatctat ttacacgttc tatttagata
     6661 cgttaattgc tagaaatagc aaagaagctc aaaagttgag tctttatctt aatcattctg
     6721 gtaaaagtag ttctctaaaa ttagtgcaag gtgctattca cttgcctaaa cgtgctgcta
     6781 aggtcacagc tatcacctca gtaaaagtag tcaaggctcc tatcaagctg gtccatgcta
     6841 tcaaagattt tctggattaa ggcttgtaaa tttttgaaaa cttgctataa ttatcttgaa
     6901 gacaagttag attggacaga atcttttaag cgagtgctgg ttggtggaaa gagcacaagg
     6961 atagctaatc tgatactcct tcctggtttt tatgcaaaca taaaagggtg gcccccttat
     7021 cgccactaga ggtgcaaggc atgttttttg tcctgcataa attaaggtgg aaccacgctg
     7081 attgcgtcct ttgggatgcg gtcagttttt ttgttttgga ggggattagc cttgttttta
     7141 aggactaggg agggtagatt cgtgaataag gtattgttct tctacgtggt ggtgttgagc
     7201 tattcactaa cctcttgtcc ctaatttgtc ttactatcca tgttttagtg gaaatcttaa
     7261 ctgacttaaa ggagaaagca aatgattaag attacttttc cagatggcgc cgtgcgtgaa
     7321 tttgaatctg gcgtaacaac gtttgacatt gctgagtcca tcagcaaatc attggctaaa
     7381 aaagctttag ctggtaagtt caatgatcaa ctcattgaca ccactcgtgc cattgaagaa
     7441 gatggcagta ttgaaatcgt gacacctgat cacaaagatg cttatgaggt gcttcgtcac
     7501 tctgcagccc atttatttgc ccaagctgca aaacgcttgt tcccaaatct tcatttggga
     7561 gtgggccctg ccattgcaga aggcttttac tatgatacag ataatgcaga aggccaaatt
     7621 tcaaacgaag atttgccacg tatcgaagct gaaatgcaaa aaatcgtgac agaaaattac
     7681 ccatgtatcc gtgaagaagt gaccaaagaa gaagctttgg aattgttcaa agatgatcct
     7741 tacaaggtag aattgattaa tgagcatgct ggtgctggct tgacggttta ccgtcaaggt
     7801 gaatttgtgg atctttgccg tggccctcat gtgccatcaa caggccgtat ccaagtcttc
     7861 cacttgctaa atgtggcggg tgcttactgg cgtggtaaca gcgacaataa catgatgcaa
     7921 cgtatctatg gaacagcttg gtttgataaa aaagacctca aagcttactt gactcgtcta
     7981 gaagaagcca aagaacgtga tcaccgtaag cttggtaaag agcttgactt gttcatgatt
     8041 agtcaagaag ttggtcaagg cttgccattt tggttaccag atggagcgac tattcgtcgt
     8101 acacttgagc gctacatcac agataaagaa ttagcatcag gctaccaaca cgtttacaca
     8161 ccaccacttg cttctgtaga attgtacaag acatcaggtc attgggatca ttatcaagaa
     8221 gatatgttcc ctgtaatgga tatgggagat ggtgaggagt ttgttcttcg tccaatgaac
     8281 tgcccacacc acattcaagt gtataaaaat catgtgcgtt cttatcgtga attgcctatt
     8341 cgtattgccg agcttggcat gatgcaccgt tacgaaaaat caggtgccct atcaggactt
     8401 caacgggtac gtgaaatgac cttgaatgat ggccacattt tcgtaacacc agagcaaatc
     8461 caggaagaat ttcaacgagc ccttcaatta atcattgatg tttatgctga cttcaacttg
     8521 actgattatc gttttcgttt gtcatatcga gaccctaacg atactcataa atattatgac
     8581 aatgatgaga tgtgggaaaa tgctcaaagt atgcttaaag ctgcacttga tgagatgggt
     8641 gtagattact ttgaagcaga aggtgaagca gccttttatg ggcctaagct tgatattcag
     8701 gtgaaaacag ctcttggtaa cgaagaaacc ctatcaacca ttcagttgga cttcttgttg
     8761 ccagaacgct ttgaccttaa atacattggt gcagatggcg aagaacaccg cccagtcatg
     8821 attcaccgtg gtgttatctc aactatggaa cgtttcactg ctatccttat tgaaacttac
     8881 aagggtgctt tcccaacttg gttggcacct caccaagtaa ccgtgattcc aatttcaaat
     8941 gaagcccaca ttgactatgc ttgggaagta gcaaaaaccc ttcgtgaccg tggtgttcgt
     9001 gcagatgtcg atgaccgcaa tgaaaaaatg caatacaaga ttcgtgcgtc tcaaaccagc
     9061 aaaattcctt accaattaat tgttggggac aaagaaatgg aagacaaatc agtcaatgtt
     9121 cgccgctatg gtagcaaggc aactcacact gaatctgtgg aagaatttgt ggaaaacatc
     9181 ttggcagaca ttgctcgcaa gtcacgtcca gatgcacaag cttaatcaat ggcaaaaaga
     9241 ggtccttatg ggcttctttt tctttcttaa aaattaagca aacgattgca cagtcttttt
     9301 catggtgtta taataaattg tttagtaacg gtgataagac gataacagaa acgaggaatg
     9361 gtaaatggtc atgatagaag tgtcccacct tcaaaaaaat ttttccaaaa ccattaaaga
     9421 gccaggatta aagggagcct tgaagtcctt tgttcatccc caaagagaaa tctttgaggc
     9481 ggttaaggac ttatcttttg aggtacccaa agggcaaatt ttaggtttta ttggagccaa
     9541 tggtgcagga aaatcaacaa cgattaagat gttaacaggt attttaaagc ctacatcagg
     9601 ttactgtcgc attaacggaa agattccaca agataatcgt caagattatg tgagagacat
     9661 tggagctgtt tttggtcaac gaacacaact atggtgggat ttggccttgc aagagaccta
     9721 tgtcgtgtta aaagaaattt acgatgtgcc tgaaaaagcc tttcgcaagc gcatggattt
     9781 tttaaatgat gttttggacc tgaatgagtt tattaaggat cctgtccgga ccttatcact
     9841 cggtcagcgg atgcgagctg atattgctgc ttccttgttg cataatccta aagtattgtt
     9901 tttagatgaa ccaacgattg gacttgatgt ttctgtgaag gacaacattc ggcgtgccat
     9961 tactcaaatt aatcaagaag aagagacaac tattttactg acgacccatg atttgagtga
    10021 tattgaacag ctctgtgatc ggattatcat gattgataaa gggcaagaaa tctttgatgg
    10081 aacggtgacc caattaaagc aaagctttgg aaaaatgaag agtctgtctt ttgagttgaa
    10141 gccagggcag gagcaggtgg tctcacagtt tatgggctta cctgacatca cagtggaacg
    10201 gcatgagctt agtttagaca ttcaatatga cagttcgcgc tatcaaacag cggacattat
    10261 tcaaaaaacc atggctgatt ttgcggtcag ggatttgaag atgactgatg ttgatattga
    10321 ggatattgtt cgtcgtttct accgaaagga gctttaagat gtggtcattt tggaaacgtt
    10381 atagaccctt tctcagtgca ggaattcagg aattaataac ctatcgcgtt aatttctttt
    10441 tgtatcgtat tggagatgtc atgggggctt ttgtggctta ttacctctgg aaggctgttt
    10501 ttgattcgtc taagcaatcc ttaatcaatg gttttactct gtctgacatg accttttaca
    10561 ttatcatgag ttttgtgacc actttactga ccaaatcaga cagctccttt atgattggcg
    10621 aagaagtgaa agatggttct atcatcatgc gcctgcttcg accagttcat tttgcagcct
    10681 cttacctctt tatggaaatt ggttttcgct ggattgtgct aatgagtgtc ggctttcctt
    10741 ttttaatggt cttatctggt attaaagtca tggcggggct gtctatcttg caagtcctag
    10801 ccagcagttg tctgtattta gttagccttt tattagcttt tttaatcaat ttttatttca
    10861 acatttgttt tggcttttca gcatttgtct ttaaaaacct ttgggggtct aatttattga
    10921 aaaatgcctt ggttgccttt atgtcaggca gtttgattcc attggctttt tttcctaaag
    10981 tggtttctat agtgcttagt tttctaccgt tttcctcttt ggtttacacg ccagttatga
    11041 ttgtcattgg gaaatacagc cttagtcaga taatggtggc cctaagtttg caaatctttt
    11101 ggttactggt gatggtagtc ttatcgcaag tcatctggaa aaaggttcag taccatttaa
    11161 cgattcaagg agggtgagat ggcaaaattg agacgtatgc acgctatttt tatcaaacaa
    11221 tacctcaaac aaattatgga atacaaggtt gattttgtgg tgggagtgct aggtgttttt
    11281 ctcactcagg gacttaatct gctcttttta tcggtacttt tccaacatat cccttcttta
    11341 gaaggctgga cgtttgaaca aattgccttt atttatggct tttctttgat tccaaaggga
    11401 attgatcacc tcttttttga caatctttgg gctttagggc aacggcttgt ccgcaaagga
    11461 gaatttgaca agtacctcac tagaccaatt agtcctttat ttcatgtttt ggttgagaca
    11521 tttcaagtgg atgccttggg tgaattattg gttggggtta tcttattagt gaccacggct
    11581 ggtagtatcg tatggactct tcctaaggtg cttttgttta ttttggttat tccttttgcg
    11641 accttgattt acacctcttt aaaaattgcg accgccagca tttctttttg gaccaagcag
    11701 tctggggcag ttatctatat tttttacatg ttcaatgact tttctaagta ccctatgtcc
    11761 atttaccatt cctttttgcg gtggttaatc agttttatca ttccctttgc ttttacagct
    11821 tattaccctg cttcttattt tttaacaggt caacacctgc tctttaatat tggaggcttg
    11881 gtagtggtgt cgttactggt tttggccttg tcgcttaagc tttggaaatg gggcctggat
    11941 gcttatgaaa gtgcaggttc ctagagagac tcttaaacaa attgaaaatc gtttaggtca
    12001 gaatatgaaa atctagataa tgaaggaagg cagctgttgt aaaaacggct gctttttttt
    12061 gtgatggttt accaagtgta ggcattggtt ggaaccatca tcagcagatt ggtctaaatt
    12121 aagggcggta aaataacata agcagatata tttcaagtgg ttgcatgcaa ttgctagata
    12181 acgtttatgg aactttgaga tcttttgcct ttatgagacc tgtcaaatct agtaacgata
    12241 ccagcttagc agatgccttg caggctgata cagacatggc ttttatttta gttagaggca
    12301 ttaactatca ataaatagta aagacaaggt ttgtcaatac cataaaaaag ttaagtgtca
    12361 cagcttagct tttttatggt gtctaattct ttattctata taagattttc ttttcttagg
    12421 aatggttttc cagtataata aagtcaccct gtatcaaaaa caaaggttac agtttgaatg
    12481 ttaaagcaac aaagtaagtg acttctacga caatagttgt gggacttgac atttcaatga
    12541 cgttagagaa ttttatagat tttattgatg gcacactagc gagactaaag agattctcta
    12601 aattaattta ggtgtatttt tagcagttat tagttcaaaa aagaaaacct tcagaaataa
    12661 ttatataata accatttaag gacatcgaaa ataaaggagg tcaatataat gacggatgtt
    12721 tatattgctg caggccttag aacccctatc ggtttagtag ggaaacaatt tgctaaagaa
    12781 caaccagaaa tccttggagc aaagctcatc aacgctttac aaaataagta tccagttccc
    12841 attgaccaag tgatttgtgg caataccgtc ggaacgggtg gtaatattgg gcgcttgatg
    12901 accctgtatt ctcatttagg agaatctgtc tctgctttga cggttgatat gcagtgtgca
    12961 tcagctggtg cagctctttc cgtgggttat gctaaaatca aggcaggtat ggcaagcaac
    13021 cttttagtgg gagggattga aagcagctcc ttacaacctg aatcggtata tgcttcagct
    13081 gattggcgcc aaggagccta caaggtagct cagttttctc cagatagtat cagccctttt
    13141 gcgatgattg aaggggcaga acgagtggca agagagcatg gctttacaaa ggagtatttg
    13201 aatcactgga cattgagaag tcatcaaaag gctagttatt gccaggagca agccctgtta
    13261 gctgatctta ttctagacct gccaggagcc agtgatcaag gtattcgacc gcgcttgtca
    13321 agtaaggtgt tgtcaaaggt accccctatc ttaggagaag atcacgtgat tagtgcagcc
    13381 aatgcctgcc taacccatga tgctgctgcc tttttacagc taagtagtca gccttcagct
    13441 tttaaactga ttgatgtggt agaagtagca ggagacccac agcgtagccc tttaatggtg
    13501 attaaggcta gtcaagtcct tttggaaaaa catggtctag ggatggcaga tatgacagcg
    13561 attgaatgga acgaagcctt tgccgttatt gatggtctat ttgaaaccca ttatccagat
    13621 ttattggacc gctataatat atttggtggt gcattagctt atggtcatcc ctatggtgcc
    13681 tcagcagcca ttataatatt gcacttaatg agggctttag agattaaaaa tggacgctat
    13741 ggaatagctg ctatagcagc agctggtggc caagggtttg ctgttttact aaaatatcat
    13801 aaggagttta gtcatgctga caaagctaga atattgggca aagcaatgcc caaataaaaa
    13861 agctatcgta gccgatcaga taagcttgac ttatcaagag ctatggcaag cggttttaat
    13921 aaaggatcag acaataaaag acagtgtacc atatatcatc agtcatagtc gctatcttaa
    13981 tcagttactc tcttttttac gaggattaaa agaaggcagt tgtcctatca ttttacaccc
    14041 taatatctca ggtacatttc agcagcaaat aaaacacgtc gatggtgagc ttcttaaaaa
    14101 agctgatttt gctgttttga gttcgggaac aactggcaaa gcaaaattat tttggcgtcg
    14161 tctatcaact tggacgagac tttttgatta tcaaaataag gtgtttggta tgactggtaa
    14221 tagttgtcta ttcttacatg gtagttttag ttttactggc aatcttaact tagcattagc
    14281 tcaactatgg gccgggggat gtctggtttt aagccaaaag ctgtctttga aaacgtggtt
    14341 aagcttatgg caagcaaaaa aggtgagtca cctttacctt ttaccgacct acctaaatcg
    14401 cttgctacct tatttgacta agaacaacat gactgcgact cacttattga cctcttcgca
    14461 aatgatatca caagaattac taagacatta ttacaaaaaa tttcctcagc ttgaaattgt
    14521 cattttctac ggggctagtg aattatcttt tataacgtgg tgtaatggca gagctgctgt
    14581 caaaattaat ggtttggtag gacagccctt tcctgatgtg tctatcagtt tcaaagataa
    14641 ggaaattttt gtagagactc cttatagtgt agaaggaatg tcacaaccat acagtgtctc
    14701 agacttagga aaaatgagcc cagtgggttt aattttagaa gggcgacaag atgactgggt
    14761 taatcaacga ggagtgaaat gccacctgcc tagcctagtt gaactcgctc accaagcgcc
    14821 aaacgtcaaa gaagcgcacg ctttaaagat aggaaaagga gaaaacgaaa ccctaatatt
    14881 agtattagtt ttgactaaga aggattgcct agcacctatt aaagactttt tagctcttta
    14941 tttaaactct ggacaacttc ccaagtacta tcttgtgatt gattgtttgc ctttaaaaga
    15001 taatggtaaa attaatcgag aagttctttt aaataaaata cccaaacagt ggcttagtta
    15061 accacttcag gtttcgtttt gcttatagtg agatacaagt gacgattcag ctttagatgt
    15121 gttataatat ggtcaaaaaa gatgaggaga aggccatgaa acaagctatc ttagatcgct
    15181 atcaggcctt gaaatgctat caaaacgcag gtttatcaaa ccaagcattt cgtgctatag
    15241 ctaaagagcc tatcattgat aatcgacttg gttctccaac tttctgggta atatggccta
    15301 ttgaaaaaga aaaccaatca gctaagcagt tactaacgtt tcttcttgat ttggtagaga
    15361 tgccatttga gctaagtggt cagttgcatg aaactcaaac gctactcacc cgattccatc
    15421 catctctatt gccagatcat atgttttgga aagagttggc tagcttagtt gatcaagctt
    15481 ttcctggcaa aacactaagc caagcaggtg agcttgaaaa gcgtttgcat caatttcgtt
    15541 atgtcatctc tagtcaacaa gctcaatcta tcagaaacca ttataaaatg attgagatga
    15601 cagacgccca agctctggcc ctttttttga ggtctaaaaa agggccttgt ttgtggcggc
    15661 aagcaccaga ttataccttg atggattctg ccaggttaca taataaacta agatttgaag
    15721 ataataaagt catttttcca agtcaagaag tttcctataa tataaaagta cttctttggt
    15781 ttcataccga atttattcta gatagcacag gcttttttct taatgaagtt gatgcagaag
    15841 tagtgaccga aaaaggaatt gtcaatggtg catcttttaa ctatggcaca gatggtccta
    15901 gacattggga tttagatgtt gatccgatta gccgtcacga tcctcagttt agaagggaca
    15961 cccttaaagg atttcgatcg ccaaaaagag tatttcgtca gtggtttaga gctcaaaaag
    16021 atgactttat gttctcttat tttaatgcca aaggactttt tgcttaccac aacaagagta
    16081 gttttgccag agtcaaaaaa tctgctaagc aattcaaacg tcaaattcat cctataaagg
    16141 gttggtttta gaaagtactc tatttgtaac ggtagtcgtc attgtcaatt aggctgaatt
    16201 tttggtataa tcaggtagaa ataatttaga aaaatatagt tttgtgattt tttattaacc
    16261 acaaaaatca caaattgtaa tatcaattac aagaactttc tgaggtatag acatgaaaaa
    16321 aatacttatt gtggatgatg aaaaaccgat ttctgacatt attaagttta atttgacaaa
    16381 agaaggttat gacattgtta cagcttttga tggacgcgaa gcggtaacaa tttttgaaga
    16441 agaaaagcca gatttaatta ttcttgattt gatgctccct gagttggacg gtcttgaagt
    16501 agccaaggaa attcgtaaaa ccagtcatgt cccgattatt atgttgtcgg ctaaagatag
    16561 tgagtttgac aaggttattg gacttgaaat tggggctgat gattacgtga ccaagccctt
    16621 ttctaatcgg gaattgctgg cacgtgtcaa ggctcatctg cgtcgtaccg aaactattga
    16681 aactgctgtt gcagaagaaa atgcttcttc aggtacacag gaactaacca ttggtaattt
    16741 acagatttta ccagatgcgt ttgttgctaa aaaacatggt caagaggtag agttgaccca
    16801 tcgtgaattt gaactattgc atcatctagc taaccatatg ggtcaggtga tgacacgaga
    16861 acacttattg gaaattgttt ggggatatga ttattttggc gatgtgcgca cggttgatgt
    16921 gactgttcgt cgtctccgtg aaaaaattga agacacacca agtcgtcctg agtatatttt
    16981 aacaagacgt ggtgttgggt actacatgaa atcttatgac tagagatatc attggaaact
    17041 tatccacatt tgaattagcg atccttatct tacttgtttt tgttgctttt tactttatcc
    17101 atcttgcggt gcgtgactac cgaaatgcac gtattattcg gatgatgagc cataaaatcc
    17161 gagacttgat taatggtcgc tatactgata taatcgacga aaaagcagac attgagttaa
    17221 tggagctttc agaccagtta aatgacctgt cagatgtttt tcgcttgacg catgaaaatc
    17281 ttgcccaaga aaaaaatcgc ttggcaagta ttttggctta tatgtcagat ggtgtacttg
    17341 ctacagaccg gtctggtaaa atcatcatga ttaacgagac agctcgcaag caattaaatt
    17401 taagtaaaga agaggcacta aagaaaaaca ttacagattt gttagaaggt gatacttcat
    17461 atacctaccg tgatttggta tccaaaacac cagtggtaac tgttaatagc cgaaatgata
    17521 tgggtgagtt tgtctcatta cgtttgcgct ttgcgttgaa taggagagag agtggtttta
    17581 tttcgggctt ggttgtggtg ctccatgaca ccacagaaca agaaaaagaa gaacgtgaac
    17641 gccgtctttt tgtctctaat gtaagtcatg aattaaggac ccctttaact tcggttaaat
    17701 cctacttgga ggctcttgat gaaggtgcac ttaaagaaga tattgctcca agtttcataa
    17761 aagtttctct tgatgaaact aatcggatga tgcgtatgat ttcagatctt ttaaacctgt
    17821 ctcggattga taatcaagta acccaattag cagtagagat gactaatttt actgctttta
    17881 taacttctat tttaaacaga tttgatttgg ttaaaaatca gcatacaggt acaggaaaag
    17941 tctatgaaat tgtaagagat taccctatta cctctgtctg gcttgaaatt gataatgata
    18001 aaatgacaca ggttatcgag aatattttga acaatgccat taagtattct ccagatggtg
    18061 gaaaaattac agtccgtatg aaaacaacag atacccaatt aattatttcc atttcagacc
    18121 aaggactagg tatccctaaa acagatttgc ctcttatttt tgatcggttc tatcgtgtag
    18181 acaaggcaag aagtcgtgcc caaggaggga ccggcctagg ccttgccatt gctaaagaaa
    18241 tcatcaagca gcaccatggc tttatctggg ctaagagtga ctatggtaaa ggatcgacct
    18301 ttactattgt cttgccttat gaaaaagatg cagccatcta tgaagaatgg gaggaagatg
    18361 tagactaatc aatgaatgag agtggtttta aatatagtat tttagcatct gggtccacag
    18421 gaaattgttt ttatttagaa acacctaaaa aacgcttatt gattgatgca gggcttactg
    18481 gtaagaaaat taccagtctt cttgctgaaa ttgaccgcaa acctgaggat ttggatgcta
    18541 ttttgattac ccatgagcac tctgaccata ttaagggtgt aggcgttatg gcccgcaaat
    18601 accatttgga tatctatgct aatgaaaaaa catggcagct gatggatgag tgcaatatgc
    18661 taggtaagct tgatgcctca caaaaacata ttttccaaag ggataaagtt ttgacctttg
    18721 gagatgttga tatcgaaagt tttggagtca gccatgacgc tattgatcct caattttatc
    18781 ggattatgaa agataataag tcttttgtga tgttgacaga tacaggatat gtcagtgata
    18841 ggatgacagg tattattgaa aatgcagatg gctacttgat tgaatccaat catgatattg
    18901 aaattttgag atcaggctct tatccttgga gcttgaagca gcgtatttta tctgatttag
    18961 gacatttatc aaatgaagat ggagcgggag ccatgattag aagcctgggg tacaatacca
    19021 aaaaaatata cctaggtcat ctgagtaaag aaaataacat caaagagtta gcgcatatga
    19081 cgatggtcaa tcaactggct atggcagatt tagcagtagg tacagacttt acggtccatg
    19141 atacctctcc agatactgct tgtccattaa ctgatatttg agataagaaa ctaaaagaga
    19201 atatacaaag tagcgctgat tggtgatata ccagtcaggg ctattttttg atgtcttaaa
    19261 gcgtattgtc ttctgagttc aatgaactct cattggtcat cctttagagc atcaatagac
    19321 ttaaattagc ctgtagtttt ggcttgtctg atctatttat caaaggtgtt gaggattata
    19381 aagataaggt agttttttag ttgttcagac taaaaacaaa atagctctat actatctgta
    19441 gtgggaaatt cccctcagat agtgtagagt cttttgattg tatgagaaag tttcctttga
    19501 cgggtattta agatgtttaa taaatcacga aaaatccact tagagtcggt tttctaggtg
    19561 gatttttggt atactaaata aggtctactg acaaatgtga aagggatttt ttatgaaaca
    19621 gcttgaagag ttactctcaa catcttttga tattcaattt aatgatttga ctttactaga
    19681 aacagctttt acccacacct cttacgctaa cgagcatcgt ctcctaaacg tttcacataa
    19741 tgaacgcttg gaatttttag gagacgctgt tctacaattg attatttcag agtatttatt
    19801 tgcaaagtat cctaaaaaga cagaaggaga tatgtctaag ttacgttcca tgattgtacg
    19861 tgaggaaagc ttggcaggtt tttcacgatt ttgttctttt gacgcttata tcaaactagg
    19921 aaaaggtgag gaaaagtcag gtggtcgccg tcgtgatact attttaggag acttatttga
    19981 agccttttta ggtgctcttt tattagataa ggggattgat gcagtacgtc gcttcttgaa
    20041 acaagtcatg attcctcagg tggaaaaagg gaattttgaa agggttaaag actataaaac
    20101 ttgtcttcaa gagtttttgc aaacaaaagg tgatgtggtt attgattatc aagtcattag
    20161 tgagaaagga cctgcccacg ctaaacaatt tgaggtatcg atagttgtta atggtgctgt
    20221 ccttagtaaa ggtttaggta agtctaaaaa attagcggag caagatgctg ctaaaaatgc
    20281 ccttgcccaa cttagtgagg tttagatgtt tttaaaagaa attgagttgg aagggttcaa
    20341 gtcctttgcg gacaagacta aaattgaatt tgataaggga gtaactgcag tcgtaggacc
    20401 aaatggttca ggtaaaagta atattacaga aagtttacgt tgggctttag gagaatcaag
    20461 tgctaaaaat ctgcgtgggg gcaagatgcc agatgtcatt tttgctggga cacaaaaccg
    20521 taatcctcta aactatgcta aggtagcggt tgttcttgat aattccgatc attttataaa
    20581 aactgccaag aaagaaatcc gtgtcgaaag gcatatttac cgaaatggag atagtgacta
    20641 tctgattgat ggtcgcaagg taaggctccg tgatattcat gatttgttta tggatacagg
    20701 gcttggtcgt gattctttct ccattatttc tcaaggtcgt gtagaggaaa ttttcaatag
    20761 taaacctgaa gaaaggcgcg ccatttttga agaagcagct ggtgttttaa aatataaaac
    20821 acgtaaaaaa gaaactcaga ttaaattaaa tcaaactcaa gataatttag accgtctaga
    20881 ggatattatt tatgagctag acacgcagtt ggcaccactt gaaaagcaag ctaaagtagc
    20941 taaacaattt ttagaacttg atgccaatcg taagcaacta caactagata ttctagtcaa
    21001 agatattgat atagctcaag aaagacagac taaagatact gaagctttag ctgctttaca
    21061 acaagactta gcatcttatt acgctaagcg tcaatctatg gaagaagatt accaaaaatt
    21121 caagcaaaaa aagcaggttc ttagtcaaga gtcagatcaa acccaaacaa cattattaga
    21181 attaaccaag ctaatagctg acttggaaaa gcaaattgaa ttggttaagc tagaaagtgg
    21241 ccaagaagca gagaaaaagg cagaagccaa aaaacatctc gaacagttac aagaacaatt
    21301 ggatggtttt caagcagaag aaaagcagcg tacagaacaa ttattgcata ttgatcaaca
    21361 actttgtgat gtcaaacaac agttaaacga attgagcaat gcattagagc gtttttcaag
    21421 tgatcccgac caattaatgg aaactctaag agaagagttt gtattattga tgcaaaaaga
    21481 ggcggcctta tcaaatcagc tgacagcttt aaaagcccat ttagacaaag aaaaacaggc
    21541 tcgtcaacat acggctcaag aatatcagct acttgttact aagcttgatc aacttaatga
    21601 cgagtctcaa aaagctcaag ctcattataa agctcaaaaa gagcaagtcg aaatgttgct
    21661 ccaaaattat caagaaggtg ataaatgcgt tcaggaatta gaaagagatt accaactgaa
    21721 tcaagaaaga ctatttgact tgttggatca aaaaaaagga aaagaagctc gtaaggctag
    21781 cttagaatct attcaaaaaa gtcatagtca attctatgcg ggagtacgag ctgtccttca
    21841 atcacaaaaa aagcttggag ggatcatagg tgcagtcagt gaacaccttt cttttgattc
    21901 tgattaccaa acagctcttg aaattgcatt aggtgctaat agtcagcata ttattgtaac
    21961 agacgaagct gcagcaaaaa gagcaattgc ttatttgaaa aaaaatcgcc aaggacgagc
    22021 aactttttta cctttaacta ctataaaggc tagatcatta tcagaacact atcatcgtca
    22081 attagcgact tgtgaaggtt atctgggaac tgcagaatca ttggttcgct atgatgatag
    22141 tctttcagct attatccaaa accttttatc tagtactgct atctttgaaa ctattgatca
    22201 agctaatatt gcagcgcgct tattaggata taaggtaaga attgttactc ttgatggaac
    22261 agaattacga ccaggtggtt ctttttcagg aggaactaat agacaaagca atacaacttt
    22321 tatcaaacca gagttagaac aaatcagtga ggaattaacg cgattagttg agcaattaaa
    22381 aataactgaa aaagaagtag ctgccttgca aagcgatttg atagcaaaaa aagaagaact
    22441 gacacaattg aaattagccg gtgaccaggc acgcttagct gaacaaagag ctcaaatggc
    22501 ttatcagcaa ttgcaagaaa aacaagaaga ttcaaaggct ttgttagctg cccttgatca
    22561 aagtcagacg actcattctg atgaatcttt attggcagaa caagctcgta ttgaagaagc
    22621 attaacagct attgctaaga aaaaaaatgc cttaacttgt gatattgatg atatcaaaga
    22681 aaacaaagat ttgattaggc aaaaaacaca aaacattcac caagctttat ctcaagctcg
    22741 tttgcaagaa agagatttac ttaatgaaaa gaaatttgag caagctaatc aatctcgtct
    22801 aagaactcag ctgaagcaat gccagcaaaa tattctcaaa cttgaaagca tactaaacaa
    22861 taacgtaagt caagacagca ttcaaagact accccagtgg caaaaacagt tacaagatgc
    22921 cacagagcat aagtctggtg ctcaaaaacg tttagtccaa ttaagatttg aaatagagga
    22981 ctatgaggcc cgcttagaag aaacagcaga aaaaataacc aaagaaagcg aaaaaaatga
    23041 cacatttatt cgtcgtcaaa ccaagttaga gacacattta gagcaagtgg ctaatcgctt
    23101 acgagcctat gctaaaagtt tatcagaaga ttttcaaatg acactagcag acgcaaagga
    23161 ggtaacaaat agtattgatc acttagaaag tgccaaagaa aaattacacc atttacaaaa
    23221 gactattcgt gcactaggac caattaatag tgatgctatt aatcagtatg aggaagtgca
    23281 tgagcgtttg acttttttaa caagtcaaaa gacagatctg acaaaagcta aaaatctttt
    23341 gctagagact attaatagta tggatagcga agtgaaggct cgtttcaaag tgacttttga
    23401 ggcaattcaa aaaagtttta aagaaacctt tactcagatg tttggtgggg gttctgctga
    23461 tttagtttta acggaaacag acctcttgtc tgctggtata gagatttctg tgcagcctcc
    23521 tggtaagaaa attcaatctc ttaacttgat gtcaggcgga gaaaaagctt tgtcagcact
    23581 ggctttattg tttgctatta tacgtgttaa aacgattccg tttgtgattt tagatgaggt
    23641 agaagcggct cttgatgaag caaatgttaa gcgctttggg gatttcctca atcgctttga
    23701 caaagatagt caatttattg tcgtgaccca tcgtaaagga acaatggctg cagcagatag
    23761 tatttatgga attaccatgc aggaatcggg ggtctctaaa attgtatctg taaaattaaa
    23821 agaagctcaa gagatgacca attaaaaaac agaagggagt tccttctgtt tttttggatg
    23881 ttcggccata agttaggaga tttcatgatt aaactaatag caacagaaat cggaataagc
    23941 taattgatac aatagagtac aaagaaagcc ttggaaatag cagtttccaa ggctttttag
    24001 ctgtcgatag gtaaaagaga aaagttgtca gagtagatga ttttttattg agacaccctt
    24061 gattggcgtt tcgttaaaag gtaaagaatg attttactta atctttgttg actagtttat
    24121 caaaatggtc agcgtaatgt aattttaact tgtcttcatt taaaatatca agaacttgta
    24181 tggcttgttt catcttttca actccagttt ctaattggcg cttaaattca aagtaacctg
    24241 ttgcatagag aaaaactgtc tgttcataga agtttaattc attatctaat aatgatttta
    24301 tctcatcaat taaataggag caattagaaa attcctcctt attgatacta agaatgaaac
    24361 aatttatagc aagctgagtg acaattcgtt tattagtttt gttccgtgaa gagtaaatgt
    24421 aattatttaa catctccttt gtcaatagga agtaggagtt gtagttaatt gttcgtacac
    24481 agttacccaa caaaaggatt tcatagtagc cccatttttc aactttaaag agataatctg
    24541 ttagttgaag taattcatca tctgatggaa tgatgcttga atccattgtg tgtaaaatgg
    24601 actttaccat tgttttctca aaagaattca gagtgtaatc agaagtgtcg gaaagtaaac
    24661 atgctatatt attgatattt tgtgatgaat attgcttacg aatatactgt accaggttag
    24721 caaatgtttc ggaattggta tagtctttat catgtaaaat aagaaactca tccaaagtaa
    24781 tgtgtaattt gtctagaata ttgatcagtc ggatacacga tatctctgac tccccgcgct
    24841 caaaacgaga gatttgtgat tttgaaaggt gttcatctgc cacgaagcac agactaattt
    24901 gctttccttt tcgaactttt ctaagtgtgg caccaagttt tgatttcata ttttcccact
    24961 ttcccaacaa aaatcaattc agtttttatt tattgtacaa taaataaaaa ctgaaaggaa
    25021 gtccgcttat gaagaaaatt tcaaaatttt tgccgatttt aattttagca atggatatta
    25081 tcattattgt tggtgggtaa gttacttagg tgtacaccta agtctttaga tactctaaag
    25141 acttataggt aatggttagc tctgtgtaat gcttgtactt tatgttagta gtaaactaat
    25201 taaacgtagg aggatgaaat ttatgtattc tgcattgctt ggtaatccag ttg
//
LOCUS       DATURV010000020        25228 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DATURV010000020 DATURV010000000
VERSION     DATURV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25228
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.020"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(407..793)
                     /gene="rplQ"
                     /locus_tag="VCF75_000798"
     CDS             complement(407..793)
                     /gene="rplQ"
                     /locus_tag="VCF75_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEP2546378.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(808..1746)
                     /locus_tag="VCF75_000799"
     CDS             complement(808..1746)
                     /locus_tag="VCF75_000799"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEP2546379.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1792..2175)
                     /gene="rpsK"
                     /locus_tag="VCF75_000800"
     CDS             complement(1792..2175)
                     /gene="rpsK"
                     /locus_tag="VCF75_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEP2546380.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2193..2558)
                     /gene="rpsM"
                     /locus_tag="VCF75_000801"
     CDS             complement(2193..2558)
                     /gene="rpsM"
                     /locus_tag="VCF75_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEP2546381.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2576..2692)
                     /gene="rpmJ"
                     /locus_tag="VCF75_000802"
     CDS             complement(2576..2692)
                     /gene="rpmJ"
                     /locus_tag="VCF75_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEP2546382.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2718..2936)
                     /gene="infA"
                     /locus_tag="VCF75_000803"
     CDS             complement(2718..2936)
                     /gene="infA"
                     /locus_tag="VCF75_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEP2546383.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3054..3692)
                     /locus_tag="VCF75_000804"
     CDS             complement(3054..3692)
                     /locus_tag="VCF75_000804"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEP2546384.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEELGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3842..5146)
                     /gene="secY"
                     /locus_tag="VCF75_000805"
     CDS             complement(3842..5146)
                     /gene="secY"
                     /locus_tag="VCF75_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEP2546385.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5163..5603)
                     /gene="rplO"
                     /locus_tag="VCF75_000806"
     CDS             complement(5163..5603)
                     /gene="rplO"
                     /locus_tag="VCF75_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEP2546386.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5818..6000)
                     /gene="rpmD"
                     /locus_tag="VCF75_000807"
     CDS             complement(5818..6000)
                     /gene="rpmD"
                     /locus_tag="VCF75_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEP2546387.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6015..6509)
                     /gene="rpsE"
                     /locus_tag="VCF75_000808"
     CDS             complement(6015..6509)
                     /gene="rpsE"
                     /locus_tag="VCF75_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEP2546388.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6528..6884)
                     /gene="rplR"
                     /locus_tag="VCF75_000809"
     CDS             complement(6528..6884)
                     /gene="rplR"
                     /locus_tag="VCF75_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEP2546389.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(6989..7525)
                     /gene="rplF"
                     /locus_tag="VCF75_000810"
     CDS             complement(6989..7525)
                     /gene="rplF"
                     /locus_tag="VCF75_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEP2546390.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7728..8126)
                     /gene="rpsH"
                     /locus_tag="VCF75_000811"
     CDS             complement(7728..8126)
                     /gene="rpsH"
                     /locus_tag="VCF75_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEP2546391.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8277..8462)
                     /locus_tag="VCF75_000812"
     CDS             complement(8277..8462)
                     /locus_tag="VCF75_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEP2546392.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8478..9020)
                     /gene="rplE"
                     /locus_tag="VCF75_000813"
     CDS             complement(8478..9020)
                     /gene="rplE"
                     /locus_tag="VCF75_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEP2546393.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9044..9349)
                     /gene="rplX"
                     /locus_tag="VCF75_000814"
     CDS             complement(9044..9349)
                     /gene="rplX"
                     /locus_tag="VCF75_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEP2546394.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9428..9796)
                     /gene="rplN"
                     /locus_tag="VCF75_000815"
     CDS             complement(9428..9796)
                     /gene="rplN"
                     /locus_tag="VCF75_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEP2546395.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9821..10081)
                     /gene="rpsQ"
                     /locus_tag="VCF75_000816"
     CDS             complement(9821..10081)
                     /gene="rpsQ"
                     /locus_tag="VCF75_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEP2546396.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10107..10313)
                     /locus_tag="VCF75_000817"
     CDS             complement(10107..10313)
                     /locus_tag="VCF75_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEP2546397.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10323..10736)
                     /gene="rplP"
                     /locus_tag="VCF75_000818"
     CDS             complement(10323..10736)
                     /gene="rplP"
                     /locus_tag="VCF75_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEP2546398.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10740..11393)
                     /gene="rpsC"
                     /locus_tag="VCF75_000819"
     CDS             complement(10740..11393)
                     /gene="rpsC"
                     /locus_tag="VCF75_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEP2546399.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11406..11750)
                     /gene="rplV"
                     /locus_tag="VCF75_000820"
     CDS             complement(11406..11750)
                     /gene="rplV"
                     /locus_tag="VCF75_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEP2546400.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11766..12044)
                     /gene="rpsS"
                     /locus_tag="VCF75_000821"
     CDS             complement(11766..12044)
                     /gene="rpsS"
                     /locus_tag="VCF75_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEP2546401.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12183..13016)
                     /gene="rplB"
                     /locus_tag="VCF75_000822"
     CDS             complement(12183..13016)
                     /gene="rplB"
                     /locus_tag="VCF75_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEP2546402.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13034..13330)
                     /locus_tag="VCF75_000823"
     CDS             complement(13034..13330)
                     /locus_tag="VCF75_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEP2546403.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13330..13953)
                     /gene="rplD"
                     /locus_tag="VCF75_000824"
     CDS             complement(13330..13953)
                     /gene="rplD"
                     /locus_tag="VCF75_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEP2546404.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(13977..14603)
                     /gene="rplC"
                     /locus_tag="VCF75_000825"
     CDS             complement(13977..14603)
                     /gene="rplC"
                     /locus_tag="VCF75_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEP2546405.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14851..15159)
                     /gene="rpsJ"
                     /locus_tag="VCF75_000826"
     CDS             complement(14851..15159)
                     /gene="rpsJ"
                     /locus_tag="VCF75_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEP2546406.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15358..16647)
                     /locus_tag="VCF75_000827"
     CDS             complement(15358..16647)
                     /locus_tag="VCF75_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEP2546407.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKLPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(17035..18051)
                     /gene="adhP"
                     /locus_tag="VCF75_000828"
     CDS             complement(17035..18051)
                     /gene="adhP"
                     /locus_tag="VCF75_000828"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEP2546408.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNIGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVAPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18303..20945)
                     /gene="adhE"
                     /locus_tag="VCF75_000829"
                     /gene_synonym="adhC"
     CDS             complement(18303..20945)
                     /gene="adhE"
                     /locus_tag="VCF75_000829"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEP2546409.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSTHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIVDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKTADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
     gene            complement(21254..23029)
                     /locus_tag="VCF75_000830"
     CDS             complement(21254..23029)
                     /locus_tag="VCF75_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEP2546410.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLLWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMIGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGSLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
     gene            complement(23026..23427)
                     /locus_tag="VCF75_000831"
     CDS             complement(23026..23427)
                     /locus_tag="VCF75_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546411.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            complement(23451..23888)
                     /locus_tag="VCF75_000832"
     CDS             complement(23451..23888)
                     /locus_tag="VCF75_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEP2546412.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMNKEY"
     gene            complement(24026..25024)
                     /gene="ruvB"
                     /locus_tag="VCF75_000833"
     CDS             complement(24026..25024)
                     /gene="ruvB"
                     /locus_tag="VCF75_000833"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEP2546413.1"
                     /translation="MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            complement(25071..25166)
                     /locus_tag="VCF75_000834"
     CDS             complement(25071..25166)
                     /locus_tag="VCF75_000834"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HEP2546414.1"
                     /translation="MLKKYKYYFIFAALLSFKVVQELSAVDWWRL"
BASE COUNT         7430 a         5330 c         4445 g         8023 t
ORIGIN      
        1 taacaatata gtttgagcat ctgtttgcga tgcaagtatt ttttcgaaca tttaaatgag
       61 aggattacac cgtttatttg cccatgagag gtctttaaac acctgcataa ggttttctta
      121 ttctaatgac cttattgtct ctgagaaaac tttataaacg catcagaaga ggcttctgtg
      181 ttattgtttt actcagctta aaagcgggag tgtcacctca taatacaaca aaacgcctgc
      241 ttataaaaag tttctatagc ttagatacct ttcataaaca agcgttacat attaacaata
      301 tgatgagtgt tcccgctagg aaaaatccta gcggtagacc agagctagac taagaataat
      361 ctctcgatta ctccatcatc ataacactca acaaaattga taaaaattaa actaattcaa
      421 taattgccat tggagcagca tctccacggc gtggttctgt tttaagaata cgagtgtatc
      481 caccgttacg ttctgcataa cgaggtgcaa tttctgagaa aagtttttga agagctgtcg
      541 ttgatgtgta tttatcggta gcttcatcat aattttctga tgcgatttcg ttacgaacgt
      601 atgcagctgc ttggcgacga gcatgaagat caccacgttt accaagagta atcatttttt
      661 caactgtttt acggatttct tttgcacgtg cttctgtcgt tacaattgat tcgttgatca
      721 aaagatctgt cgtcaaatca cgaagcattg ctttacgttg tgagctagtg cgtcctagtt
      781 tacggtaagc cattttgtcc tcctatatta tttatcgttt tttagtccga gacctaagtc
      841 agcaagttta accttaactt cttcaaggct cttacgtccc agattacgga ctttcatcat
      901 ttcaggctca gatttttctg ttaagtcgaa gacagtgtta atgcctgcac gttttaaaca
      961 gttatatgag cgtacggata aatcaagctc ctcgatggtg cggtcaagta ccttttcatc
     1021 gttcactttt tcagtttctt tcattacttc ggtagctttt gcaacctctg ttaaatcagt
     1081 aaacaggttt aagtgttcga tcaaaactcg agcagataga cctaaagcat cttcaggaat
     1141 aattgttccg tttgtcatga tttcaatcgt taatttatca aagccatcat tgctacctac
     1201 acgggcaggt tcaacttgat aattaacttt tttaactggc gtatagattg aatctactgc
     1261 caatgtaccc acaggtgcat catctttttt gttgccttct gctggcacat aaccacgttt
     1321 tttagcaaca gtcattgttg cacgtagaga atgtccttca gcgattgtaa aaagataatg
     1381 atccgggtta acaagttcga tatcactgtc agtaagaata tcaccagctg ttacctctgc
     1441 tggtccttct acttcaagtt cgataatctt ttcgtcttcg acgtatgatt tcacagcaag
     1501 tcccttaaca ttaaggataa tttgcatgac atcttcacgt acacctggga ttgtatcaaa
     1561 ttcgtgtagt actccatcaa ttttaattga tgttactgct gcacctggaa gtgaagacaa
     1621 gagtacacga cgaagagaat tacctagagt tgttccgtag ccacgttcaa gtggttcgat
     1681 gacaaatcta ccgtaatctt tattttcatc aatttttgtt attattggtt tttcaaactc
     1741 aatcattttt tcacccctcg aaacgaaact tgtgtactat ttgttatatg attatacacg
     1801 acgacgtttt ggaggacgag caccattatg tggcacaggg gtcacatcac gaattgcagt
     1861 tacttcaaga cctgcagctg ctagagcacg aatagctgat tcacgacctg aaccaggacc
     1921 ttttacagtc acttcaacag tttttagtcc gtgttcttgt gcagattttg cagctgcttc
     1981 tgcagccatt tgagcagcaa atggagtaga tttacgagaa cctttgaaac caagagcacc
     2041 agctgatgac catgcaagag cattaccatg cacatctgta atcataacaa tagtgttatt
     2101 aaatgtagcg tgaatgtgtg caacaccaga ttcgatgttc tttttcacac gacgtttacg
     2161 tgttggttta gccaattgtt ttacctccta ttttattttt tcttacctgc aatcgcaaca
     2221 gctttccctt tacgagtgcg agcattgttt ttagtatttt gtccacggac aggaagtcca
     2281 cgacgatgac ggattccacg gtatgaaccg atttccatca aacgtttaat gttcatgttt
     2341 acttcacgtc gaaggtcacc ttcaactttg attgcatcca cttcacgacg gatagcatct
     2401 tcttgatctg atgttaaatc tttaacacgg atatcttcag aaatacctgc agctgctaag
     2461 atttttttag atgttgcaag accaattccg taaacataag taagtgaaat tactacgcgt
     2521 ttatcatttg gaatatcaac tccagcaata cgagccattt tttctccttt ctattttatc
     2581 cttgacgttg tttgtgtttt ggatttgtcg gacaaattac cataacacga ccattacggc
     2641 gaataacttt acagtattcg caaattggtt taaccgatgg tcttaccttc atgttttaat
     2701 ccctccaatt atttcgatta tttaaagcgg tatgtgatgc gtccacgtgt caagtcataa
     2761 ggactcattt ctactgtaac acggtcacct actaaaatac gaatgtaatt tttgcggatt
     2821 tttcctgata cagttgctag aatttgatgt ccattttcca attcaacagt aaacattgca
     2881 tttggcatcg tttctactac tttgccttca atttcaatca cgtcttcttt tgccacgcaa
     2941 aagtaccccc taaatttcga tttgatgtcc tctgagcaca gaggtaacaa ttataagtca
     3001 gactaaacta gtatatcatt actaatctac ttatgcaagt agattagtat aatttatttg
     3061 agttctagca acgctttttc aacatctgca aaaacgtctg ttatttcttg attaccttca
     3121 atatctgtaa caagaccaag cttacgatag tgttcaagaa taggttctcc ttgagccata
     3181 ttgacgtcca aacgacgttt aacagtttca ggcttatcat cttcacgttg atagtaatct
     3241 tcttctttat aatctactgg tgggttgaac actttatgga acgtttcacc agttttacga
     3301 ttgataatac gaccactcaa acgctctaca agacatgatg gatccacttt aatattaata
     3361 acaccatcta agcggagtcc tagttcttca agtgttgcat ctaaggcgtg tgcttgttca
     3421 atagtacgtg gatacccatc aagcaaaaaa cctttttctg cgatatcatc ctcagctaag
     3481 cgctctttta caatcccgtt tgtgacttca tcaggaacca attcaccttt atcaatataa
     3541 cttttagcta aacgtcccat ttcggtttga ttagccattg cggcgcggaa catatcccct
     3601 gttgagatgt gagcaacacc aagttcttca acaatcttag ctgcttgggt ccctttacca
     3661 gctcccggca aacccatgat taaaagattc atcttttcct cctcttttaa aataaataca
     3721 tagtaggaaa ttattttcct tggtttttgt tttcaaatgg caacaagaaa tcgtttgatg
     3781 ataaccaagt cttattgtca tttaaaaaca aaatagaagg ttgacaatgt caacctgtat
     3841 tctattctgc tgtattcata aatccgacat attttctctt tagaagatac ccctcaagct
     3901 gtttcatacc ttcgatacca gttgaaatca aaatgagcaa actagttcca cctaaagcaa
     3961 tactagaaga aaggttgaga gcttgttgtg ctgctattgg cgctaaagag ataaatgcta
     4021 agaagatagc tcctacggtt gctagttttt ttagcaatgc ggacataaat tgttctgtct
     4081 cacgtccagg gcgaacactt ggtatatatg aggaattctt ctgaagattt tctgctgtct
     4141 tctcaggatt aacttgtaca aaggtataga agaatgagaa caatataatc aacaaggcgt
     4201 aaactatcat tccgactgga gtttgataat taaaaatttc ttgcaaacgg tttaaccacg
     4261 gtaaatctct accattttga acaaaaggaa taatcgtact tgggatagtt gtaatcgagc
     4321 tagcaaagat aacgggaata acgccggctg gatttacttt taatggaaga tatgaacttg
     4381 taggtgcacc ttgcattagc tttgtgtatt ggattggaat tttatattcc gcttgttgga
     4441 catatgttgt aaagaaaaca atagcaagaa cagctatgat taaaatccca acaatgagat
     4501 aagacgagtg taaatcactt gccttaacat ttacaaaata gtcttcacga attgttgcaa
     4561 ttgcacttgg aatagacgaa ataatacccg caaagataat cattgatacg ccattaccaa
     4621 atcctttatc tgtgatttgt tctccaagcc aggtaacaat aacgctacct gttgtcagta
     4681 atgccccgat cagcaaatat gttttgatgt ctggtgtctt aacaagtgca acgttcgaca
     4741 aagtattaaa tcctgcagtg ataccaatgg attgggcaaa agccaaaaca agtgaaatat
     4801 agcgcgtcgc ttgatttaat ttacgacgtc caacctcacc ttgtttgccc cactcaacaa
     4861 acttaggtaa aatatccatc tgcaataact gaacaacgat agaagcagta atatatgggc
     4921 taacccccat tgaaaatact gagaaatttc tcatcgcatt accactaact aagtttaaca
     4981 tatttaagaa agggagttca cttaattgct ctaagctctt ggcatttaca ccaggtacgg
     5041 taatatgtgt cccaattcgg aatacaagaa tgataaatat agtaaagaaa attttattcc
     5101 gtacagtctt tatctttaac gcgtctttta gtatttttaa gaacataatg agtatcccct
     5161 cattagatga cttcgattga accaccttta gcaataatag ctgcttcagc agattttgag
     5221 aacttagctg cttttacagt caatttttta gtcaattcac cgttaccaag cactttaaca
     5281 cctgattttt cagctcggac aataccagca tcttttaaaa tagccggagt cacttctgta
     5341 ccatcatcaa aaacattcag ttgatcaaga ttcacaagtg catattcttt agtattaata
     5401 tttgtaaagc cacgttttgg aatacgacgg aacaatggtg tttgtccacc ttcaaaacct
     5461 aaacgtacac cgccaccgct acgagctttt tgaccttttt gaccgcgacc agatgtttta
     5521 ccattacctg atgatgtccc acgaccaaca cggttacgta ctttacgtga gccttcagca
     5581 gcttttaatt catgaagttt cattttttct cctttttttg taaaatgcta gcgcctctat
     5641 gagggaaaag gactccctat agaaactcgc ctatacatta atgatactta agttatccaa
     5701 aataacttaa tacagatacg cactaaccga ctactgtttg ttgccaaaaa agcaaactac
     5761 aagagtcggt tttgtgctcg tttttaatta tgaaatcaga gatttcacag ttataactta
     5821 tttaacatct tcaacggtaa ccaagtgaga aatagctgtc accataccac ggatagcagc
     5881 attatcttct ttgactactg aagagtttaa tttaccaagt ccaagagcaa caacagtttt
     5941 acgttgttct ggcttacgtc cgataggaga cttagtcaaa gtaattttaa tttgagccat
     6001 gatgatcccc tttcttatgc taagtcagaa actgagatgc cacgaagtgc ggcaacttct
     6061 tctgcacgtt taagttgttt caatccttca acagttgcac gaacaatgtt gattggagtg
     6121 tttgaaccaa gagattttga agtaatatca gcaacacctg ctaattcgat gacggcacga
     6181 actgcaccac cagcagcaac tccagaacct tctacagctg gtttcaataa tactttagct
     6241 ccaccgaagt tagtgtaaac ttcgtgaggg attgttgtac caaccattgg tacttcgatc
     6301 atgtttttct tagcagcttc aactgcttta cggatagctt ctggaacttc ttgagcttta
     6361 ccagtaccaa atccaacacg accatttcca tcaccaacaa ctacaagagc tgcaaagcga
     6421 agacgacgtc cacctttaac aacttttgta acgcggttaa tcgcaacaac gcgttcttca
     6481 agttcaactg cattatcttt aaatgccatt attttgtgtc ctccctatta gaatttcaat
     6541 ccgttttcac gagctgcatc agccaaggct ttaacacgtc cgtgatagag atatccaccg
     6601 cggtcaaaca ccacttcaga aataccttta gccactgcac gttcagcaac aagtttgccg
     6661 actacaacgg cttgttctgt ttttgttcct ttagaaacgt ctttatcaag agttgatgca
     6721 cttgcgagcg ttacacccgc tacgtcatca ataacttgag cgtagatgcc tgtattagaa
     6781 cggaatacgt tcaaacgtgg gcgatcagca gttccagaga gtttaccgcg gacgcgacgg
     6841 tggcgttttt ggcggatttt gtttttatct ggtttcgaaa tcacaatttt cacctcttaa
     6901 ttttaatcaa tttgtctcat tgacaagttg tcgattgaac ttatcgagca tgttaaggtg
     6961 caatacttcc catacccgac taagaacatt atttacctgt tttaccttct ttaaggcgta
     7021 cgtattcacc aacgtaacgg atccctttgc ctttgtaagg ctctggtgaa cgcaagctac
     7081 ggatgtaagc agctgtttga ccaacaactt ctttgttgat tccttcaact gagattgaag
     7141 ttgggttagc aacagtgaaa gtaattcctt ctggagcttc aacttcgtct tggtgagatt
     7201 tacctactga aaggacaagt ttagtacctt gaagttgagc acggtaaccg acacccttca
     7261 tttcaagatc ttttttgaaa ccttcagaaa cacctacaac catgttattc aagttagcac
     7321 gggttgtacc atggattgtt ttcatttctt ttgagtcgtt aggacgtaca actgtgattt
     7381 cagtcccttc aactttgatt tcaatatttt tgttaaactc acgagtgagt tcgcctttag
     7441 ggcctttaac agtaataaca ttgttgttat ttgttaattc aacgcctgca ggcatagtaa
     7501 ttactttatt accaatacgt gacatgttta ttttctcctg ttaaattgtc aagccgcata
     7561 gcgactagtt ttcacggggg tttgatagaa tgatatactt caatttatat ttcaaaattg
     7621 aacacgagct gtggtctgtg tgaaaaagat aaatcttcct tgagtataaa cacgctacgt
     7681 cagatttcct attttcactt tgtccacgac gctcttagta tcttaaatta ccaaacgtaa
     7741 gcgatcactt ctccaccaac attcttttgg cgagcttctt tgtcagtcaa aagaccttct
     7801 gatgttgaaa tgattgcaat tccaagtccg ttaagaactt taggcatgtc atcacgtttt
     7861 gcgtaaacgc gaagacctgg ttttgaaata cgttttaagt tagtgataac acgctcacca
     7921 ttttttccgt attttaagaa cacacgaatg atgccttgtt tatcatcttc gataacttca
     7981 acatttttta caaaaccttc acgtttaaga atctcagcaa tccctttttt aatgtttgaa
     8041 gcaggtactt ctaacacttc gtgttttact tgattagcat tacggatacg tgttaaaaag
     8101 tctgcaattg ggtcagtcat aaccattttt tatattctcc tcttactagc agtttgatgc
     8161 tatcacttac tagttaatgt tgtccttaaa ggactttggt atcagcttat gctcacaccg
     8221 ttttaattaa gcacaagact taatctgaag aacaaattaa gttttgtgtc atatttttac
     8281 caagaagctt tgacaacacc tggaatttga cctttgtaag ccaattcacg gaagcaaaca
     8341 cgacaaagtt taaacttgcg gtaaactgaa tgtgggcgtc cacatttttc acagcgagta
     8401 taagcttgtg tagagtgttt tgcaggacgt ttgttcttag caatcataga ttttttagcc
     8461 aatttattta ccccctatta ttttgcaaaa ggcattccaa ggcccttaag caattcacgt
     8521 gattcttcgt cagtatttgc agtagtgacg attacaatat caagaccacg tactttatca
     8581 acatcatcaa agctgatttc tgggaagata agttgttctt tcacaccgag agtgtagttt
     8641 ccgcgtccat caaacgattt tgttggaacg ccatggaagt cacgtacacg aggaagtgaa
     8701 acgctaacca atttgtctag gaattcgtac atacgttcgc cacggagggt aacttttgca
     8761 ccgatcgcta caccttcacg aagacggaag ccagcgattg atttcttagc tttagtaata
     8821 agtggttttt gacctgagat aagtgctaat tcagctgcag ctttctctag gttttttgcg
     8881 ttggatacag catcaccaac acccatattg agaacaattt tctcaacttt tggcacagcc
     8941 ataactgatg tgtaattgaa tttctctgtc aacgcaggaa ttacttcgtt agtatatttt
     9001 tcttttaaac gatttgccat tatgcttctc ctttccttcg tgattaatca agcacttcgc
     9061 ctgatttttt gctgtaacgt acttttttac cgtcaacaac tttataacca acacgtccag
     9121 ctacaccatt tttatctaat acttgaacat tagagacatg gataggtgct tctttttcga
     9181 cgatagctcc ttgaggattt tcagtgttag gtttttggtg ttttttaatc attccaacac
     9241 cttcaacaat aactttgtta acttttggaa gtgctttaag aactacagct tcagtgcctt
     9301 tgtccttgcc agcaattacg cgaactttgt cgcctttttt tacaaacatt ctggtttctc
     9361 ctattatttc ttacgccccg atgggcaccc taggaaaacc taggggacta cgtttgttaa
     9421 cctgagatta aagtacttct ggtgcaagtg atacgatctt catgtagcca ccctcacgca
     9481 attcgcgtgc aactgggcca aagatacgag ttccgcgagg agttttatca tcacggatga
     9541 ttacagcagc attgtcgtca aatttgatgt atgaaccgtc tggacggcgt gcaccagttt
     9601 ttgtacgaac gataacagct ttaaccacat cacctttttt aactgctcca ccaggagtag
     9661 cttgttttac agaagcaacg attacgtcac cgatgttagc gaatttacgt cctgaaccac
     9721 caagtacttt gatagtcaag atctcacgag caccgctatt atcagcaact ttcaagcgag
     9781 tttcttgttg aatcatttca attttctcct tttagtttga ttagataata acagcttttt
     9841 ccactacttc cacaagacgg aaacgttttg tagctgaaag tgggcgagtt tccatgatac
     9901 gaacgatatc gccttcttta gcaacgttgt tttcgtcatg tgctttatat tttttagaat
     9961 agttgatacg tttaccatag actgggtggt tacgtttagt ttcaactaca actgtgattg
    10021 ttttgtccat cttgtcagac actacgcgtc cgtaaagagt tttacgttga ttacgttcca
    10081 tttagaattt ctcctttccc aatctattat ttcatttctg attgcacagt tttaacacgt
    10141 gcaatttgtt tcttaacttc gtcaaggcga gcagtctttt caagttgacc tgctgcagct
    10201 tggaaacgaa gatcaaacaa ttcttttttg agttcgtttt ctttcttagc aagttcttct
    10261 tgagacaatc cacgaagctc tttaacaaaa tctttaattt cttgaagttt catgtcttct
    10321 ccttattctg cttcacgttt tacgaatttg cacttaactg gtaatttgtg gctagcaaga
    10381 cgtaatgctt cgcgagcaat ttcttcagaa acaccagcaa tttcaaacat cactttacca
    10441 cgtttaactg gtgctaccca accttcaggt gcccctttac cagaacccat acgtacaccg
    10501 atagcttttg cagtgtatga tttgtgtggg aagattttaa tccaaacttt accaccacgt
    10561 ttcatgtaac gagtcatcgc aatacgggca gcttcgattt gacggtttgt aatccagtgg
    10621 ctagttgtgg cttgaagtcc gtattcaccg aatgaaactt ctttaccacc tttagcctca
    10681 ccgcgcattt ttccacggaa ttcacgacgg tgtttaacac gtttaggtac taacatttgt
    10741 tatttgcctc ctttagtgtt tttacgagct ggaagaactt ctccacggta aatccaaact
    10801 ttaacgccaa gtttaccata agttgtgtca gcttcttccc aagcatagtc gatatcagcg
    10861 cgaagcgtgt gaagtggaac agttccttct gaataacctt cagcacgagc gatatcagca
    10921 ccgttcaaac gaccagaaac ttgagtttta atccctttag ctcctgcacg cattgtacgt
    10981 tggattgctt gtttttgagc acgacggaaa gcaacacgtt gctcaagttg acgagcaatg
    11041 ttttcaccaa caaggtgagc atctaaatca ggttgtttga tttcgatgat gttgatgtgt
    11101 acttgttttc cagttaattt gttaagttga ccacgaagag cgtcaacgtt tgcgccacct
    11161 ttaccgataa ccatacctgg tttagcagtg tgaagtgaaa caataacttt gtttactgca
    11221 cgttcaattt caatagttga aactgatgcg tcagctaatt ctttattaat gaatttacgg
    11281 attgcaagat cttcatgaag gtaatccgcg tattcttttt cagcatacca tttcgcatcc
    11341 cagtcacgga tgattccgac acgcatacca attggatgta ctttttgacc cacgatttta
    11401 cctccttatt tttctgatac aactacagtt acgtgagttg tacgtttgtt gattggtgaa
    11461 gctgaacctt tcgcacgtgg acggaaacgt ttcattgttg gtccttcgtt agcgaatgtt
    11521 tcagatacta ccaagtttgc tttttccaaa ccaaagttgt tttctgcgtt agcaattgct
    11581 gagttaagag ttttctcaat aacacgagct gctttgtttg gagtgaattt taagattgcg
    11641 attgcgtcag caactttctt accacggata agatcaagta ctaaacgtgt tttacgaggt
    11701 gaaacacgca ctgtacgagc cattgcttta gctgaagtaa tttctgccat tgtgtcctcc
    11761 tcctattaac gacgtgtttt cttgtcgtca gctgcgtgac ctttgtaagt acgcgttggt
    11821 gcaaattcac caagtttgtg acctaccatg tcttcttgga tgtaaacagg tacatgttta
    11881 cgtccgtcat aaactgcgat tgtatatccg atgaaacttg ggaaaatcgt tgaacgacgt
    11941 gaccaagttt tgattacttt tttcttttcg tcgtttgctt gagcttcaac ttttttcatc
    12001 aaatgctcat cgacgaaagg tcctttttta agactacgtc ccattttgta gttttctcct
    12061 ttaaataatg taccacagcg gcttgaattc aagagcaaga ctccaaactc taccgagttg
    12121 gcggaataaa tagcaaagct attgtaacgc tatttcagca agttttaagc aacttactaa
    12181 gattattttt cgttacgacg acgaacgata agtttgtctg atttagcttt cttgttacga
    12241 gttttaagac caagcgctgg tttaccccat ggagttgatg gcgctttacg tccaactggt
    12301 gctttacctt caccaccacc gtgtgggtga tcgttagggt tcattacaga accacgaact
    12361 gttgggcgga tacctttcca acggctacgt cctgccttac caatgttaac aagtgattgt
    12421 tgttcgttac caacagtacc gatagttgca cggcatgtac caagaatcat acgaacttcg
    12481 cctgattgaa gacgaacaag aacgtattta ccttcttgac caagtacttg agcagaagct
    12541 ccagctgcac gaacaagttc tccaccttta ccaggtttca actcaatatt gtgaacaact
    12601 gtaccgacag gaatgtttgc taatggaagt gcgttaccaa ctttgatatc tgcatctgga
    12661 ccagaaacaa tacgttgacc tacttcaaga cctttaggtg caatgatgta agctttcaca
    12721 ccgtcagtgt aatgtacaag tgcgatgttt gcagtacggt ttggatcgta ttcgattgtt
    12781 ttaacaactg cttcaacgcc atctttgtta cgtttgaagt cgatcaaacg gtaatgacgt
    12841 ttgtgtccac caccttggtg acgaactgtg atgcgaccat tgttgttacg accagctttg
    12901 ctcttaagag aaacaagcaa tgatttctca ggcgtgcttg ttgtgatttc cgcgaaatcc
    12961 aaagaagtca tgttacgacg gccatttgtc gttggtttat aaactttaat acccacgtta
    13021 tttcctcctt agattattca gcttcagctg cgaacaactc gattgcttta gaatcagctg
    13081 taagagtgat gatagctttt ttagtttttg aagtgaaacc tgtgtaacga ccaacgcgtt
    13141 ttgcttttgg tttaacgtta acagtgttta cacttgcaac tttaactccg tcaaaagcag
    13201 cttcaacagc ttgtttgatc aaaagtttgt gtgcacgagt atcaacttcg aaagtgtatt
    13261 tgcctgcttc aagagcaatc attgacttct cagtgataac tggttttttg attacgtcgt
    13321 acaaattcat tatgcaagaa cctcctcaat tgtagagatt gcttctttag taacaagaag
    13381 tttgtctgcg ttcacgatat caagaacact tgcagttgct gcagttgcaa cggttacgtt
    13441 tggaaggtta cgtgcagaaa gtgctgcaaa ttcatttcct tcttcaacaa gaacaagtac
    13501 ttttgtatca atgctaagag ctgaaagcac ttttgcaaat tcagcagttt ttggtgctgc
    13561 aaatgaaagg ccttctacag ctacaaattt atcttcagca acttttgctg agtaaactga
    13621 tttcaacgca aggcgacgaa ctttttgtgg aagtttgtat ccgtatgaac ggggagttgg
    13681 tccaaagaca acaccaccac cacgccattg tggtgagcgg atagaacctt gacgagcgcg
    13741 tccagttcct ttttgacgcc atggtttacg tccaccacct gatactgctg aacggttttt
    13801 aaccgcatga gtaccttggc gaaggctagc gcgttggcta attacaacat caaaaacaac
    13861 tgattcgttt ggttcgatac cgaagatagc gtcgtttaat tcaactgagc taacttcttt
    13921 accagtttgg tcaaatagtt ttacgtttgc cattttaact gttttctcct ttcttattat
    13981 ttagcagctt taactgctga cttgatagtg ataagagatt tcttagcacc tggtacgtta
    14041 cctttaataa ggataacgtt cttttctggg ataacttgta caatttcaag gttttgaact
    14101 gttacacggt taccacccat acgtcctgcc aagcgtttgt ttttgaaaac gcggttaggc
    14161 gcaacaggtc ccatagaacc tgggcgacga tggtaacgag aaccgtgagc cataggacca
    14221 cgtgattgac catggcgttt gataacacct tggaaacctt taccttttga tgtccctgtg
    14281 acgtcaacaa catcaccagc ttcaaattgt tctacagata attctgcacc aacttctaag
    14341 ccttcaatgt ttttgaattc acgaatgaag cgcttaggag ctgtgtttgc ttttgcaaca
    14401 tggcctttgg caggtttgtt actcaagact tcacgtttgt catcaaaacc aacctgaact
    14461 gcttcataac cgtctgtttc aacagtttta acttgaagca caacgtttgg agttgcttca
    14521 atgacagtaa cagggatgaa ttcgcctgat tcagtgaaga tttgagtcat tcccactttt
    14581 ttccctaaga ttccttttgt catgagaaaa tatttccttt tcttaataat atttcaaaaa
    14641 gttttttatg agcgtttttt gtgctcaaaa aagtttttta cgagcgtttt ttgtgctcat
    14701 agcaagatac aaagagcgtc aaactcaaaa taaaaataga aaatctaaca caaaaacttc
    14761 tgtttcgagg aagatttatc tttttcatgt agagtttagc tcgtgttcaa gttccatcaa
    14821 acactaactg tacttgcaaa atctcaccga ttaaagtttg atttctacgt tgacaccact
    14881 tggaagatca agtttcatca aagcatcaac agttttttgt gttggattga tgatgtctac
    14941 caaacgtttg tgtgtacgca tttcaaattg ttcgcgagaa tctttgtatt tgtgagtcgc
    15001 acgaataatt gtgtaaagac tgcgttcagt tggaagtgga actggtccag caactgtagc
    15061 acctgtacgt gttgcagttt caacgatttt ttctgccgct gtatcaagtg tacggtgttc
    15121 gtacgctttc aaacggatac ggattttttt gtttgccatc ttttctcctc tcgtctattt
    15181 acgataatag gctagctcct caagaaaacc gacacctgtt gcgtggcaat gcaaccgagc
    15241 gtgtcgcaac ctcttgcatc attgctaaag ctgtattttt acagcaccac aatataataa
    15301 cagaatcatt tagtcattgc aagcttttta gggcattttt tcaaaaaaac taccttccta
    15361 atgaaaggta atttcttggt actttttaaa ttgtttagag agaataaacc accgactggt
    15421 attatctaac actgtcgcca tccagacccc tatgagtcca tattgccaaa ccacaccaat
    15481 cacataacca aggccaatgc gaatcaccca cattcctatg gttgttgcat agaaaggaag
    15541 ctttgctttt cccaaaccct gccagacggc tgtataaact aaagtgcctg ccgttgcggg
    15601 agctcctagt aaagaaaata gtaaaacaac catggcggac ctttgagcat cagtattctg
    15661 agtaaatagc ggcaagagac taggacctag cagataggtc aaggctccca taaccagcat
    15721 catgagggtg gataaaataa aagcttctct aataatatac cttatctcag tcaccttacc
    15781 gccccccagt tgcctggcca ctagaataat tgttgccgtt gccatggcta agccaggcat
    15841 gtaattaaac tgagtcaagg tttctccaat agcattacct gccaaggccg ttgtcccaaa
    15901 gcgaacaaca atgccaataa ttaggacatc tcctgctctc atcatcaaac gttcaccagc
    15961 agcaggtagt gataaatcaa aaataatttt gtccaaaggt ctcatcaatc gtttcgcaac
    16021 ctgtttaata ggaataaact gacaaagtaa aaacacaccc actaaacgag ataaaaccgt
    16081 cgcccaagca acaccaagaa gaccaaatcc ccatacatag atagacaagg cagaaaaaat
    16141 ggcattgagt acgtttatta ataagctcac ttgcatagga agcttaggat acccttgagc
    16201 tcgaacaata gctcctaagc tagttaaaag tcctagacta acaatcatac cgccgacaat
    16261 ggatagatat tgccctccaa ccaatgttac agaagcttca gctcctagcc attctaacac
    16321 ctgatgatgt ccaacaactg ataacaaccc taagccgacc gaaaggagga gtgttacttg
    16381 caagacacct gccatatagt ttaattgttt gttctgatta ttttctccaa tacttcgagc
    16441 aatcagacta gatacagcag ctcccagcgc gataaagagg gactgataaa tactgataat
    16501 gttgttagct atggagacac cagagactgc tacaagtcca atttgagcca ccaggtaatt
    16561 atcaaccatt cccattaaca tctgtaaaat attttctatc atagaaggca gagctaaaga
    16621 gaaaattttt cttctattat atatcattta tgtcccctaa taaaaaattc ttgatcaaac
    16681 gattttttca aacacagtca ctggactcat tcagttattt tggtccatta atgaattcat
    16741 aaagtctatc ttaatagcag agtcccaaga tacacgacac ttagtgctac agtaacaatt
    16801 gaccacatgc caatccttaa catttaccta acgtttataa ctaagaagat gaagaacaaa
    16861 aggactccaa acgaattttt tctattatat aatatattct tcatttagca actttcaatg
    16921 tatcaaaata gcactctcga gtcaaaaatg ttttctgata ctcgagagtg ctattttatt
    16981 tggaatcaag tttaagcaac ttaggcttat ctcatattta atacatttgt atgtttagct
    17041 gatgaaatct aagaccttgc gcccttggat taaaccacgt tccatttcat caaagacttc
    17101 tggagcagtg tcaactggaa ctttttcgac aaccggagca accagacctt ctgcaccaaa
    17161 ggcaaaggct tcttccaagt ctttacgtgt ccctacaagt gatccgacca ctttgatacc
    17221 atctaaaaca gtttttacga tagacagttc catgtattct gatggaagac ctacggctac
    17281 aactgttcca ccggcacgga cactatcaat tgcttggtta aaagcaactt tagaaacagc
    17341 cgttaccaca acaccatgtg ctccaccggt tttttcttga atatagcctg gaacatcttc
    17401 aatttctttg ccgttaacta aaatatctgc gccaacttct ttagctaact caagtttatc
    17461 attattaatg tccactgcga caacatgtgc attaaagact tttttagcgt attgaacagc
    17521 cagatttcca agaccacctg ccccaaagat aacaatccat tggcctggag cagcacctgc
    17581 ctctttaata gctttataag ttgttacacc agcacaagta atggaagaag cttgagccgg
    17641 gtctaaacct tctggaactt taactgcata atcagctgtt actactgcat attcactcat
    17701 tcctccatca acactgtatc cagcattttt aacactacga caaagtgtct cacgtcctgt
    17761 agtacagtat tcgcaatgac cacatccttc aaagaaccat gcaatagaga cacgatcacc
    17821 aacctttaag gatgttacgc cttctccaat ttcttctact ataccaatac cttcgtgccc
    17881 caaaatacgt cctggtacct gaccaaaatc gccatgtgca acgtgtaaat cagtatgaca
    17941 aacgccacag tattctactt taacaagtgc ttcaccatga ccaatatttg gaagatcatg
    18001 ctcaacaact tcgacacctg tactagcttg attaacaact acggctttca tatcagtcct
    18061 ccatctaata atgacttttt tgtaatcggt tacacataaa ttgtacatct tttcacaatg
    18121 aaagtcaata gaaacctgaa aagtttctga aacttaactg gttaatctta tagctgtttg
    18181 cctatctgca aaatacttaa ctacattttc tgacactgga tataaatgat atatttagaa
    18241 aataaaatga tttcttatga ctttaatcat aagaaatcat ttcctggtat caacattaaa
    18301 aatcacttac gtcgtcctgg acgttcagca taaccatagt aagcatcttc aataatttct
    18361 tgcatgtgat caaccattgg aagacgtggg ttagcaggtg aacattggtc ttcataagca
    18421 aggtaagcca attcacgaga atgttctttc cattcatttt cgtcgatacc ttgagctttg
    18481 aagttcattt gaataccaac tcggcaaccc agatcataaa cagctttcgc ataagattcc
    18541 actgcttctt ctggtgtaga agctggaagc cctaacaatt tcgcaatgtc ttggtatttt
    18601 tcatccgcac ggtagtaatt atacttaggc catgtcgccg ttttagctgg acgagttcca
    18661 ttatagcgga taacatacgg caaaagaatt gcatttgtac gtccgtgtac ggtatggaat
    18721 tgtgcgccaa ttttatgcgc cattgagtga gaaataccta agaatgcatt ggcgaaagcc
    18781 atacctgcca tagttgacgc attgtgcatc ttttcacgtg cttcaaaatc ggctgtttta
    18841 actgattttt caagattatc aaaaacaatt ttaatggctt gaagagctaa accatccgta
    18901 aagtcattgg ccatttgtga cacgtaagct tcggttgcgt gtgtcaaaac gtccatacca
    18961 gtatcagcag caataaagcc tggaacagtc aataccaaag ctgggtcaac aatggctaca
    19021 gttggtgtta atgagtaatc agcgattgga tatttacgat tatttgcttt gtcagagata
    19081 acggcaaatg gtgtcacctc tgaacctgtt ccagaagttg ttgggatagc aacaaatttt
    19141 gttttcttac caagttctgg gaatttgaac gcacgtttgc ggatatccat gaatttttga
    19201 acgaggtcat ggaaatcaac ttctggttgc tcgtagaaga gccacataac tttggctgca
    19261 tccataggag atcccccgcc aagggcaata atggtgtctg gtttgaaggt acgcattaat
    19321 tcagtacctt tcataacagt tgtaatgtct ggatctggtt ctacttcagc aaagatctga
    19381 taaacaactt tattgcgacg aagatcaagt tgttcaatga tacgatccaa gaaaccaagc
    19441 tcaaccatag cgtgatctgt aacaatcatg acacgttcaa cgtcacgaca tttttgcaaa
    19501 tattgaattg aatcacgttc gaagtatgtt tttgaaggaa ctttaaacca ttgcatatta
    19561 tttctacgtc ttcctacttt tttgatgttc aagagattta tagcactaac gttatcacca
    19621 actgcgttac gtccatacga accacaacca agagtcaatg atggcaagaa agcattgtaa
    19681 acatccccga taccaccaaa tgtagaaggt gaattccaga tgacgcggat agcacggatt
    19741 ctagttccaa attcttttgc caactctgca tctgctgtat gaatggcagc tgagtgaccg
    19801 agtccattaa attcaaccat ttgacgagct ttttcaacac cgtcttcacg tgattcagat
    19861 ttcaaaacag caatgactgg agataatttt tcacgtgtta aaggctcatt ttctgaaact
    19921 tctttacatt ctgcagcaag aatattggtt ccttctggaa cagtaaatcc agcttgttct
    19981 gcaatccaag tagctggttt cccaacaatg tttggattca atttagcgcc agcacagttt
    20041 ttactgttag cttttgcacc aaaacagaat tcttcaagaa gggctttttc ttttttattt
    20101 acaaaataag tatggtaaga tttgaactct gcaacaaaat cgtcatagat ttctttgtcc
    20161 acgataacag cttgttcaga cgcacagacc ataccgttat caaatgattt agacatcaca
    20221 atatcatgag cggcttggcg gatgttagca gatttttcaa cataagctgg aacgttacca
    20281 gcacctaccc caagggcagg tttcccacat gagtatgccg ctttaaccat tgcatttcca
    20341 cctgtcgcaa ggatagtggc aatgccgtca tgattcatca aagcatttgt tgcttcaagt
    20401 gatggtgttt caacccactg aacacaattt tcaggagcac cagcggcgat agcagcgtct
    20461 ctaacaatcc gagcggcatg cgttgatgat tcttgtgctg atgggtgaaa ggcaaaaata
    20521 attggattac gtgtttttaa cgagatcaaa gatttaaaga tagctgttga agttgggtta
    20581 gttgttggtg taatcccaca gataacaccc accggctcag caattaatgt caaacctgtc
    20641 acatcatctt cttcaataat tcctactgtt ttttgatggc gcatgttatt aaccacatgc
    20701 tcacaagcaa atagattttt tgttgcttta tcttcaaaga caccgcgacc tgtttcttca
    20761 taagcatgtt tagcaagttc accgtgagca tccaaagcag caacagaagc tttggctacg
    20821 atataatcaa cttgttcttg gtcaagctta cgcatctctt caagagcagc taaacctttt
    20881 tgaacaagag catcaattgt cacagaaaca gaggttgttt caacagtatt atgtccttca
    20941 gtcatttgag actcctttat attgttaaaa gtttcacaat tttgtttaca aaattattat
    21001 agcacgtttt gaaattttgt aaatcatttc acaataaatt tgtgaagttt ttttcaaatt
    21061 atttctgaaa tcctatggta tcaatattct tcattaactc tttttcagaa atatatctaa
    21121 aaaaagccgt tttgtttctg aaaccgcttt cttttaaaaa cctcaaagta ctactatttt
    21181 accatcatca tttgttcaca agaaaaagcc agagcacaat ggatctgact tatctcttag
    21241 acatttctta actttattta gctggttttt tagctgactt ctcaacagca gtctggattg
    21301 tggagacata tagttctgct ccttgactat cttcactata atgaacaccg tcactgccat
    21361 accaaatatc tggatgttca acagcaactt tataccagtc tgcaatagtg acatacttgt
    21421 attttttgga caatttaaga ccatagtctc tagcttctgc aacctgactg gcatttttgg
    21481 cattatatgg agaaaccaaa actaaacgat gtccttttgg caatgcctca ataaaagact
    21541 gaactgcttg agaatagttg tcaagcgagt taacacctac tgctaagaca acagtcttag
    21601 atagactctt agacttaatt tggttattaa ataaatcgaa agcttcttta aaattccgac
    21661 tgaccgctgc atccagttgc gcttgtggca taatttttga gaatgctgca ctagagcgta
    21721 atgctacaga atcaccaata atacctacgt cactcaaagc attagcgtct cctgcagcta
    21781 gtgtatgtgt ccgctccata ttactttgag cctgttgtag ggaactgacc aacaattgtt
    21841 tctcaaactt accaacttta ggcgctatca tacaagttcc taaagttaat aatgctaagg
    21901 cgagagaccc tcctacaatc cacttatagt aaggagaaca gtcaatttct aaaccaagca
    21961 ggtttggttt tctcccttgg accaaaggtt ccacaatata gtaagacaca gtggcaaaca
    22021 agattgaaaa gaaaactgtt agaataactg ccaaaatatg agacattaat tggctaaaaa
    22081 taatgtaaaa tggccaatgg aagaggtaaa tcccatagct aatatcagct atataagtga
    22141 tagctttagg ctcttgtaca tctggtgttt gctcatgaag aacacgcgct gcataaatca
    22201 taattgatgc gaataagcta gccaaagcaa agccaaatag ataggtcgtg atatggttaa
    22261 aatccaatgt gaccgttagc acaagcaaaa gtgtaaatgc tccaatcata gcagcaagga
    22321 catactggcg cggccataaa cgtacatttt tttgaaatcg aacagtagtc tcgttaatac
    22381 ctgttattgt ggcaaacatc gcccctaaaa agaagggaaa actgtgagaa agactcgaaa
    22441 aataaatcag ggaaaaattt gaggtcataa aagatcttat aaacatgctc aagaaactaa
    22501 tagcaaatat tcccaaagaa atcaaaaaca gtagtcctct cagttgtttc tgagtctcct
    22561 tgcgtctagc taataaccaa acaaatacac cccacaacaa gtaaaaatga acttcaatag
    22621 ctaagctcca cgtatgaaca aagagatgtg gaataaattg gctttcataa ctacttcccg
    22681 tcaatatttc ataaatatta gtggtaaatc ctaaaacagc tgtaatctga cttcctatat
    22741 tagcaatgaa atccttctta attaaaaacg taaaaggaat ggtcaataaa atcattaaca
    22801 ccaaaggagg aacaattcga taaaagcgtc tttttaaaaa accgataata tcgatagact
    22861 ctttttttgt gtattcatca atcaagaggg ctgtgattag atatcctgaa aaggtgaaga
    22921 aaatatcaac accaataaaa cctcctggaa atacattttt aaaaaagtgg tagagcaaaa
    22981 ccagtaaaag tcctgttacc ctaacaaacg aaaaccattt tattctcatt tttgaaatat
    23041 cccctgttta aaaatccctt tatagacttt attattcttt gcttttaaca cacctttgcc
    23101 atttgcttgt cctttatgaa actccccgtt atataaccag cccgtcttag cagtaaaagt
    23161 tccttttcct tcaaataggc cattcttaaa agtaccctca tagatatcac cgttggggta
    23221 gacaagcttc ccttctccat tcattttatg gttaatcacg taaccagtat agtgcatacg
    23281 ccctttatca taggtcaaac ttgtcttatt cgaaattctt acgctaaaaa cagaaattgc
    23341 acaaaccaat atgacaatca ccgagacaat ttccaacttc gcccgtgtaa tactccattt
    23401 ttttactata tcttgcatat ctatcatttc ttttactcaa cttctaatta tcaatactct
    23461 ttattcatta aacgagaaag ccaatcctgg catccttttc taactaattg gtaggtttct
    23521 tcaaaatcgt tagtgtacca aggatcagga acaccccctt ctctaaacag atagatttta
    23581 gagtcccatt gatgttgcga catctctttg agatttttca cattatcgct atccatacca
    23641 ataatatagt caaaggtgtt aaaatcagtt atagttatct gcttggagca tttagttata
    23701 tcataattaa tttggtaagt ttttaatatg gactgtgtac ctgagtgaat agggtttcca
    23761 tgctcccaat cagaagtagc acgactttct atcatcataa catcacttga tactattgac
    23821 ttcattacaa actcagccat ggggcttcga caaatattac ccagacagac aaaacatact
    23881 tttttcatta aacactcctt tgaattatta tatcaaattt ctaactgata tcggagaaat
    23941 caatctaata attttctgtc aacttatcaa aaaatccgaa cacaaaaagc aggttcagat
    24001 taattttctt agatttgaaa aatcattaag tgttttgata gggatagcca agatgacgat
    24061 aagccttttg cgtcgctaca cgacctgttc gtgttcgcat gagaaagcct ttttgaatta
    24121 agtagggttc atacatctct tcaactgtat tacgctcctc ggcaatattc acagataatg
    24181 tccccaaacc aactggtcct ccctgataca tctcaatcat cgttcgtaaa attttttgat
    24241 caatgtaatc gagaccttcg cggtcaacat ctaacatagt caaagctcta tcagttattt
    24301 gagccgttat gatgccatca ccaataattt gagcgtaatc acgcactcgt tttagtaatc
    24361 gattagcaat gcggggcgtt cctctactgc gacaagctaa tttccgtgca gcttcatggt
    24421 ctatttttat ttcaaaaata gtagctgtac gctccacaat ctctgtcaaa tccttttctt
    24481 gatagtattc catgtgacca gtgataccaa aacgagctct gaggggatta gataacatgc
    24541 ctgcacgtgt agtagcacca atcaatgtaa atggaggcag atccaaatga atactacgac
    24601 tagtatctcc agcgccaatc ataatatcaa tatagaagtc ttccatagca ctatatagta
    24661 cttcctcgac agacattggc atgcggtgaa tttcatcaat aaacaaaata tcccctggtt
    24721 ctaattcatt taaaatagct accaaatctc cagctttctc aacggcagga cctgaagttt
    24781 gctttaaatt aactcccaat tcattggcaa tcacaaatgc cattgttgtt tttcccagtc
    24841 ctggaggtcc aaataataaa acatggtcta aagattcgtc acgtctttta gctgcttcta
    24901 taaaaatagc gaattgttcc ttaactttat cttgtccaat atattcgtgt agatattgag
    24961 ggcgaagagt ccgatccgaa aactcctcat ttcccattac attattgtct aaaattctag
    25021 ccataggact attataacat aaagtaaaag agagcagtgg cctactttta ttataatcgc
    25081 caccagtcaa cagcactaag ctcttgaact actttaaaag atagtagagc tgcgaatata
    25141 aaatagtact tatacttttt taacatggat aaaaactcct taataattat tttttatatt
    25201 aggaattata tcttactatt aaatattt
//
LOCUS       DATURV010000021        24390 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DATURV010000021 DATURV010000000
VERSION     DATURV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.021"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(175..1311)
                     /gene="recA"
                     /locus_tag="VCF75_000835"
     CDS             complement(175..1311)
                     /gene="recA"
                     /locus_tag="VCF75_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEP2546415.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPQLFD
                     EIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
     gene            complement(1400..2671)
                     /locus_tag="VCF75_000836"
     CDS             complement(1400..2671)
                     /locus_tag="VCF75_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEP2546416.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSNLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            complement(2740..3300)
                     /locus_tag="VCF75_000837"
     CDS             complement(2740..3300)
                     /locus_tag="VCF75_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEP2546417.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVAAFTSEEMADALENLSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            complement(3310..3906)
                     /gene="ruvA"
                     /locus_tag="VCF75_000838"
     CDS             complement(3310..3906)
                     /gene="ruvA"
                     /locus_tag="VCF75_000838"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEP2546418.1"
                     /translation="MYDYIKGQLTKITAKYIVVEANGLGYMINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLDGKFVEAPQETGNTKARSNKAGNTQLDEAIEAL
                     LALGYKATELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            complement(3908..5128)
                     /locus_tag="VCF75_000839"
     CDS             complement(3908..5128)
                     /locus_tag="VCF75_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2546419.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFDVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFQDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            complement(5139..7121)
                     /gene="mutL"
                     /locus_tag="VCF75_000840"
     CDS             complement(5139..7121)
                     /gene="mutL"
                     /locus_tag="VCF75_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEP2546420.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLVISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNTFTETDHPNLDLKNRQKLSQMLNRLE
                     NEEQSVFPGLDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGDVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            complement(7250..9805)
                     /gene="mutS"
                     /locus_tag="VCF75_000841"
     CDS             complement(7250..9805)
                     /gene="mutS"
                     /locus_tag="VCF75_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEP2546421.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFNSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDHYRKVMREGTGWIADIEAKERQASGINNL
                     KIDYNKKDGYYFHVTTSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKTLATVDVLQSLAVIAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKTHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(9792..10144)
                     /locus_tag="VCF75_000842"
                     /pseudo
     CDS             complement(9792..10144)
                     /locus_tag="VCF75_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            complement(10141..10578)
                     /gene="argR"
                     /locus_tag="VCF75_000843"
     CDS             complement(10141..10578)
                     /gene="argR"
                     /locus_tag="VCF75_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP2546422.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVSTPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            10869..12560
                     /locus_tag="VCF75_000844"
     CDS             10869..12560
                     /locus_tag="VCF75_000844"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEP2546423.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKAKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFAKMGYQPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAEIDHTVDEEARGWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            12648..12956
                     /locus_tag="VCF75_000845"
     CDS             12648..12956
                     /locus_tag="VCF75_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP2546424.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKACHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(12983..13855)
                     /locus_tag="VCF75_000846"
     CDS             complement(12983..13855)
                     /locus_tag="VCF75_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP2546425.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(13898..14776)
                     /locus_tag="VCF75_000847"
     CDS             complement(13898..14776)
                     /locus_tag="VCF75_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP2546426.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVMGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            complement(14796..15737)
                     /locus_tag="VCF75_000848"
     CDS             complement(14796..15737)
                     /locus_tag="VCF75_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP2546427.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIIPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            complement(15730..17478)
                     /gene="aspS"
                     /locus_tag="VCF75_000849"
     CDS             complement(15730..17478)
                     /gene="aspS"
                     /locus_tag="VCF75_000849"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEP2546428.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVKGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTNGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANAQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            complement(17816..19096)
                     /locus_tag="VCF75_000850"
     CDS             complement(17816..19096)
                     /locus_tag="VCF75_000850"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEP2546429.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDIYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFENISEQLLS"
     gene            19316..19498
                     /gene="rpmF"
                     /locus_tag="VCF75_000851"
     CDS             19316..19498
                     /gene="rpmF"
                     /locus_tag="VCF75_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEP2546430.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            19514..19663
                     /gene="rpmG"
                     /locus_tag="VCF75_000852"
     CDS             19514..19663
                     /gene="rpmG"
                     /locus_tag="VCF75_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP2546431.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            19957..20571
                     /locus_tag="VCF75_000853"
     CDS             19957..20571
                     /locus_tag="VCF75_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEP2546432.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGEILEKYSRWFIAVVYLGLGIYILIENNSFDMLWAVLG"
     gene            20583..20921
                     /gene="cadX"
                     /locus_tag="VCF75_000854"
     CDS             20583..20921
                     /gene="cadX"
                     /locus_tag="VCF75_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEP2546433.1"
                     /translation="MKKDSICQVNVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLTVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            21115..21888
                     /locus_tag="VCF75_000855"
     CDS             21115..21888
                     /locus_tag="VCF75_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546434.1"
                     /translation="MLYLNTQTDSDYKEIIFGDIAKFVENMFYHYFSSILFRDLETVD
                     KRMYSFSDDDLISIQSSCLRLSKTFANFNIQRKNFLASDETEMDTFHKKDDIDITVGE
                     KSYNLARSFRTSTINELTVFNFEEMFDIIWLMLGDNLIKSFEVNVCGILFELDGNGIP
                     STFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFLVDDILERMYKENIPK
                     SYLTSVPLVISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            21954..22706
                     /locus_tag="VCF75_000856"
     CDS             21954..22706
                     /locus_tag="VCF75_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP2546435.1"
                     /translation="MAIPDLDKFSSSLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            22703..23306
                     /locus_tag="VCF75_000857"
                     /pseudo
     CDS             22703..23306
                     /locus_tag="VCF75_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23782..24063)
                     /locus_tag="VCF75_000858"
     CDS             complement(23782..24063)
                     /locus_tag="VCF75_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP2546436.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGI
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRSALRIERFKKTYDVQTYR"
     gene            complement(24070..24114)
                     /locus_tag="VCF75_000859"
                     /pseudo
     CDS             complement(24070..24114)
                     /locus_tag="VCF75_000859"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7657 a         5215 c         4160 g         7358 t
ORIGIN      
        1 ggtttttcac ctgtattccc ttgaattatt agttatttta attttatatc agttaaccta
       61 agttctgctc tctcacatct ttttaaatga tggtttctat tcacccacac acctatgaac
      121 agtagttcta ctaaccttta aaaatacatt ttaaaaagcc ctgaagggct ctttttaatc
      181 ttcaatttca ataccattat ctaaatctaa agcaagatca tcggtttctt ctgatgctac
      241 tgccatagca gattcttctt cgctttcttc aagcaaacca aatttaacac gtactttacg
      301 gtcgatttca tcaaacaatt gtggatgatc ggccaaataa cgcttagcat tttcagaacc
      361 ttggccaatc ttctcaccat tataagagaa ccaagcacct gctttttgga taatgtccaa
      421 atcagaagca attttcacaa gctcccctgt acgagaaata ccttccccat acatgatttc
      481 aacttctgct accttaaatg gcggagcgac cttgttttta acaaccttga ttttggtctc
      541 cttaccaatg ctgctatctt tttggtcacc agttccttta atttgagttg ttccacgcac
      601 atccagccga acagaagcat agaatttcaa agctcgacca cctggtgttg tttcaggatt
      661 tccaaacatc acaccaactt tttcacgcaa ttggttaata aagatagcga tagtttttgt
      721 tttattaata gaagctgata atttacgcat ggcctgactc atcatacgtg cttgcaatcc
      781 gacatggcta tcgccaatat caccatcaat ttcagcacgt ggcactaaag ctgctactga
      841 atcgacaaca accagatcaa ccgcaccaga atcaatcaat ttacctgcaa tttcaagtcc
      901 ttgttctcca gaatctggtt gagacaagag gagttcatca atattaaccc caagcgcagc
      961 agcataagct ggatcaagcg catgctcggc atcgataaag gctgcgattc caccttcttt
     1021 ttgagcttgt gctacagcat gtaaagccac agtcgtttta ccggaagact ctggaccata
     1081 gatttcgatg atacgtcctt taggataacc accagctcca agcgcaatat caagagctag
     1141 acttcctgaa ctcataacct gaactttttg ctctgcacgt tcccctaatc gcatcactgc
     1201 gcccttacca aaatcttttt caatattttt caaagcatca tcaagggctt tgcgacgttc
     1261 gtcaccaaac ttcttagtga tttcttcatt cttttttaat ttttttgcca atactttctc
     1321 ctctttttta acaaaccatc gtcagcagga ttaatctaca taatcgtaaa ccatatccta
     1381 ctatagctac taaataacat tatagcaaat tctcaagttt taataaagtt ttacggacca
     1441 tattaaaggc atgtaaagta gcgatatagc gcacatccaa acgacttcgc ccgctaatca
     1501 aaacctttat tgattctacc ttattttgag tggcaagacc aatgaaaact gtacctgcag
     1561 gctgttcctc caacatatct ggtccggcaa cacctgttaa acttacccca atatcagcac
     1621 cagtcaataa ccttgcttgc tccgccatcc cctcggccgt ataagcacta acaacgccat
     1681 gcgatttcaa ctcctctaaa ggaaggccta gcattttcgc tttttcttcc atgctataag
     1741 tcacaaagcc tccattgaat acttgagaag ctcctggaaa actcgccaaa gttgactgaa
     1801 ataatcccgc ggttagactt tctgctgctg taattgtctt atcatatttt actaagagct
     1861 caaatgtctc acgcgctaag gaattatcct ccccatagcc ataaaagaca tcagctaagg
     1921 gttgaccttc aagagttttt cgggatagta gctgcgcttc tagctgacct aactttttat
     1981 ctgccagagc ttggttttca gtttttgttg ataagcgaag agtcacttcg ccagtcttag
     2041 catacggagc aatggttgga tcagtttgat tctcaataaa atctgacaag actgttacca
     2101 actgactttc cccaatacca aaaaagcgta gtacctttga atacaatgta ctgtattgtt
     2161 ttgacagaag tggtaccaat tcttcattta ccatcggctt caattcactt ggaggacccg
     2221 gtaagacaac ataactaata ccatcgactg tgatgaaccc accaaccgca agaccagttt
     2281 tatttggcaa agggattgac ccttcaatca cttgtgcctg tcgctcatta tttggtgtcc
     2341 gtgatgaagg cttgcgctta gcaaaaaagt catctagttt ctgacaagct tgctcatcat
     2401 aaaccaagtc tttcctaagg tactttgcta aagtttgttt ggttaaatca tctttcgttg
     2461 gaccaaggcc accacataaa attaccaagt tactacgctg actagcagtt gtaatcactg
     2521 aaagtaaacg ctcctcgttg tccccaacag ccgtttgaaa atagacatca atacctagct
     2581 ctgccatttt ttccgacaga aattgagcat tggtattcac aatttgacca gtcaaaattt
     2641 cggtacctac tgcaatcagt tcagctttca tttctccacc tacttatcta ttcgtaatct
     2701 gatctcattt tatcaaaaaa tcttccaatt cacgaagaat tagaagattc tattgaaaac
     2761 acaagcctct tcatggtcat ttaccaaacc cgatgcttgc ataaaagaat aaaccgttgt
     2821 aggtcctaaa aatttaaatc ctcttttctt taaatcctta gctaagcgaa tagataattc
     2881 tgtttgagca ggaacaagat tctcttgatt gacaaggttg ttaattggct ttcccccaac
     2941 aaaattccag agataagttg aaaaactacc aaactcttct tgaattttct gtactgcgat
     3001 agcattgttg acagttgctg ctagctttag cttgtggcgg ataatggata gattctctaa
     3061 agcgtcagcc atttcctccg aggtaaaggc tgcaaccgat gcaatgtcgt aatgatgaaa
     3121 aacagttctg aaagcttggc gttttttcaa aacagtcagc caggataagc ctgactggta
     3181 actttcaagg cagagtaatt caaaaaaatc tctatcatca tgtaggggtt gaccccattc
     3241 taaatcgtgg taatcacagt aaagctggtt atccttagga acccatgagc aacgtttcat
     3301 gtgaaacacc tagcctttca ttaatagttt aagggctgat ttgatgtatt gttcggctgt
     3361 ctcagaggtt ccttcaaaga aggcacgaat tttcttcaat tccgttgcct tataaccgag
     3421 ggcaaggagg gcttcaatcg cttcgtccag ttgagtattg cctgctttat tgctgcgcgc
     3481 tttggtatta ccagtctctt gtggagcctc cacaaatttg ccatctaagt caagaaccat
     3541 ttgctgcgct gtttttttac caattttagg aaatttcatt aggtacttaa tgtcactgtt
     3601 atcaatggca ttcacaagtc cctcattatc atcaactgcc acaatagcaa gagctgttgt
     3661 cggaccaata cctgatacag aaattaattt cagaaaaaca tctttttcat cttccgtgtg
     3721 aaaaccaaac aacaggtgag catcctcacg aatcacttga tgcagataaa tggttaccaa
     3781 ttggttgaca ctatctgtaa agctataagg attggcaaca ttaatcatgt agcccaatcc
     3841 attagcttca acgacaatgt attttgccgt aattttggtc aattgacctt taatataatc
     3901 gtacatatta cacctttttc atttttgcag aaacaaccgt aaaataaatc cctagcaaag
     3961 caatgactac aaataagcat tgcacgccta aaggacctgt aaaatggctg acagatacta
     4021 ggagactagc caaaatagca cctaggggtt gtgccattga cacaaaacct gtataagatc
     4081 ctatttggga atgatccatc atatcagcac gtaggacttg actagcagga acatttatca
     4141 tttccccaaa agttaacaaa acaactgata accagatagc atagaactgc gtaaaggtaa
     4201 atgccaagag catccctaga gtaaatagta aagaaccaac cacaagctga ggtaatagtt
     4261 tccatttttc cgttaattta tttactactg tcatgaaaag gacaatcagc aatgtattag
     4321 ttaaaaccat taatgataac attttagaac tagttacttg gaaacctaac acagccgttg
     4381 gctgaaaata cagtttcaaa tggactggca catagttatc catctgcatc cagatactac
     4441 cagaaaagat ggcacctaag gtaaacaaga caaaggcacg atcctgaaac acctgacggt
     4501 agttctgaaa actcgccagt aatcctttac catgatcaaa agcgtgtgtt tctggtctag
     4561 tctcatcaaa atagtagtaa gcaacgtcaa aacaaagtac attaacgagt aataaggcta
     4621 ctaacaaggc taaaaaatga tggtcataaa aaagcccaga tagccctgca ccaaacatga
     4681 cggcaatatt gataaaccaa taattgatgg tgtaaacaaa tcgacgatta ctctcatcag
     4741 tcaaatcaat taacatagct tcataggcag gaccataaaa actagaagca atctctacca
     4801 ataaaatacc cgcaaaggtt aaccaaggaa tagctgcatt aggcaaatta gctaaaatag
     4861 tcagaaacca gcctagtgtt gttcctactg acccaatcat aatgactttt ttacgaccaa
     4921 gagcatctga cagatgccca ccgtataaag ttccaacaaa tcccatcaaa ctggtaatca
     4981 tcattaagag gcctgtccaa aacgtaccaa agtaagtcgt ataatacatg gccataaagg
     5041 gaaagatact actgcctaag gtaatggtca caaagcgtac cagttgcctc agctgaatct
     5101 gcttaggaag gtttaaaaac tcttgcatct catcctctct aatattttcc tagctctcgc
     5161 aggctagtgt gattttcttg aattcgacgg aacatttttt ccatatctgc cttactaaaa
     5221 ttaatcaata cgggtctacc atgagggcag ttataaggat tttgacattg ggccaattgc
     5281 agtagcagat ttcttgctga ataatcatcc aagctatgat ttgccttgat agatcgttta
     5341 caactcatca taatagctaa ctctgctcgg taagttttga tagatacctc attggttaga
     5401 agcaacatgt cacacatttc atagacacca gatgcaatct cttcttcttt catccagata
     5461 ggatgctccc tcaaaatgaa agtattgtgc ccataaactt ctaagaagat accgacttca
     5521 tttaagagtg ccattttctc ctgtaagtta atgaaatcag aaccagaaaa ctcaaacaaa
     5581 tagggcacca acaattgctg taaactactg tcaacgtcgc caatcttatc acgataatat
     5641 tcgtatttaa cccgctcctg ggcagcgtgt tggtcaatta taaataaacc atcttttccc
     5701 tgagcaaaga gataagttcc gtgcatctga ccaaaataat ccaatccggg aaacactgat
     5761 tgctcttcat tttccaagcg attcaacatt tgagataact tttgtctgtt ttttaagtca
     5821 agattaggat gatcagtctc ggtaaaggta ttctggggac gacttgcgtg tttaaccgaa
     5881 gaaggtccta taacttcttc tgagtggggt aacagctctg ctttttcaac ctttacactg
     5941 ttgtcaacag cgccggaata aaaatcagtt tcaggtattt tttccgagac agccgactct
     6001 ttgacaaaaa agtcattctt ttgaggatca taataaagcc ccctagactg taaaggaagt
     6061 tgtgtttgct ctggtttaga gaaatgtcgt gtgcttgatt tggctaagtt ttccagagca
     6121 tctggaatca aatcttgttc cttaagactc tctgaaatag ccgtgctaat taaagccatt
     6181 aattcacgct cttttgaaat acgaacctct tgttttgtgg gatgaacatt gacatcggcc
     6241 aaatagggat caatctgaat atcaatcaca acaattggga aacgtccaac catgagctta
     6301 gaaccgtaac catcaagaat tgctcgatta agcaagaaat tcttgatgta acgcccatta
     6361 accaaaatcg tcatgtaatt acggttggcg cgtgtcaact ctggcaagct aacatagccg
     6421 gaaacttcaa aatccaaatc agcattagag ataaccagca tttttttggt agtatttaaa
     6481 ccataaatcc ctgcaattgc ttggcgtaaa tcgccagttc ctgatgtctg agttaattgg
     6541 cgaccatcac taatcagtgt gaaagcaact tctggatgtg ccaaactcaa ccggttgacc
     6601 acatctacaa tgtgagctaa ttctgcctgt aaacttttca tatatttgag acgagcaggc
     6661 gtgttgtaaa aaaggttttc aaccttaatt ttggttccag taggagttga gatcgcttca
     6721 agtgtctcaa tttccccacc agtagctata agaagagaac cgtgagtgac ttcttttgtt
     6781 gctgttttta tcgtgatttt actgatagag gcaacagacg gtaaagcctc tccacggaag
     6841 ccaagtgttc taattctaaa caaatcactc tgactcttaa ttttactagt agcgtggcga
     6901 cgcagactta aaggtaaatc ttcatgagac attccctcac cgttgtctgt aacctgtatc
     6961 atcttaaggc cagactcttc aatttcaacg gtaatctggc tacttttagc atcaatagca
     7021 ttctcaacca attccttgac aacactcgct ggcctttcta caacttcacc agctgcaatt
     7081 tggttggcga gaacttccgg taattcaata atgtttgtca taatttcctc gcattcttat
     7141 ggactattat aacacactta ataaatagcc gaaggcctcg gaccgttttt gacagaacaa
     7201 aaagatgctt tctatattga aggcatcttt ttcattttca gctgaaagac tataacaact
     7261 ttttcaattc gtaaagggtt gtcattgctt gaagcggggt catgttcatg acatcaatag
     7321 cttccagtgc ttgcctaatc tcatgagttt tctcttcatc accaaaaagg gataattgcc
     7381 cctgtctaac agcgctgctt gactcaactt gtgaagggac tgatattatc tcagtagatc
     7441 gtgactgtgt ttctaaacgg gtcagaactt cgtctgctct ctttaatagg gattttggca
     7501 gtcctgctat ttttgccaca tgaataccgt aagatttatc cgccggtccc tcagcaatct
     7561 tatgaaggaa ggtaacatcg ccatcttttt caagcgttgc tacatgaaca ttgactagac
     7621 ttgtcaactt agttgacaag tctgtcaatt catgataatg cgttgcaaat atggtcttag
     7681 caccaactct atcatggata tattcaataa ttgcctgggc taaagccata ccatcataag
     7741 ttgccgtacc tcgtcccagt tcatcaaata gaataagaga gttgtcactt gcgcgtttga
     7801 ttgcttggtt tgcttccatc atctccacca taaaggttga ttgcccagaa atcaaatcat
     7861 cagcagcccc aatacgcgta aaaatcgcat caaataaagg taaatcaaca tggtcagcag
     7921 ccacaaatga acccatctgg gccatgataa ccgttaaggc cagctgtctc atataagtcg
     7981 acttaccact catatttgga cctgtaatca gctgaatact ggtctgttgg tcaaaagaga
     8041 tactattggg aatgtattcc tgcactccca taaccttttc aacaaccgcg tgacgacctt
     8101 cttgaattgt aatcacatga ttatcattga actgcggccg gatataatga ttggtttcag
     8161 caatgactgc taaactttgc aaaacatcca ccgttgccaa agttttagcc agtttctgta
     8221 aacgattaat ataggtttca acttgagctc gaatacacat aaaaatatcg tattctaaac
     8281 tagatgactc ttccctagcc tctaacatct gaccttcaat cttagccaat tctgctgttc
     8341 cataacgttc agagtttttt aaagttgcct ttctgaaaaa atgctcaggc actaagctaa
     8401 gatttgaagt cgtaacgtgg aaataatatc catctttttt attgtaatca atttttagat
     8461 tattaatgcc acttgcttga cgctctttag cctcaatatc cgcaatccag cctgttcctt
     8521 ctcgcatcac tttacgataa tggtccaagc gctcatcaaa accagtacga ataatactgc
     8581 cttcactaat agttgctggt gcgtctggat caatggctgt tctaatcaag tattccaact
     8641 caggcaaact gtcaatatca ttgacaagtt tgtcaacaca aggactgtta aaagactcta
     8701 agatagcttt gatataaggc acttgggcta aggtatgccc caattgaagt aaatctttcg
     8761 gatttgcctt gccaaaagac acgcgactag ataagcgttc gatgtcgtaa acacctttta
     8821 aactattgct taaatcggtt cgctcaataa aagcattcag aaaaacttga ataatttctt
     8881 gacgctctaa aatagcttct ttagaaacca aaggtcgatc aatccatgag cgcaaaagcc
     8941 tcatccccat agctgtcttg gtttcatcta aaagccaata cagacttcca tgttttttat
     9001 tagtcctagc attttctacc aaatctaaac ttgacttagt ggcatacgac atctgcaaat
     9061 aatccttgat ctcatagtga accaaggctt gcaagtggct gagttctcgc atttgtgtct
     9121 tgtgaacgta ttgcaagagt tttcctgccg ctgtgagttc taccgttgtc aattggccgt
     9181 caattaaaga tttatcttca tagaccgttt cttcataaga aagcagcaaa ttcatctgct
     9241 tgaccaaaat cgtctgttct tcttcagata aatcaaaacc tagtaagact tcttttgcct
     9301 tgaggttttg gatttcgcta cgaacactcg taaagtccgc caaatctgtc acgcaaaatt
     9361 cacctgtgga tacatccata taagccaatc cataacggca accatcaaag tcaacagcta
     9421 ccaaaaagtt attggcgcta tctggcttag ctgaatccac aaccgttcca ggagttatga
     9481 cttgaacgac ctcacgcttc accaccccaa cagcttgctt tgggtcttcc atttgttctg
     9541 cgacagcaac cttgtaaccc aactcaatta acacatcaat gtattgttgg gcagaatgat
     9601 gtggcacgcc tgccatggga attggatttt ccgcattctt gttgcgactg gtcaaaccaa
     9661 tttctaagag ttgtgctgct ttgacagcgt cctcgtaaaa taattcataa aagtcaccca
     9721 tcctaaaaag caaaaaagca tctggataat cttttttgat gtccagatac tgttgcattc
     9781 caggagaaat gttagttttt gccatttgtt aactccttgt ttaactgatc ttctatacgt
     9841 ttggtgatat actgagtcac atcacttgca tcttgcatct tttctcgata atcatcaatt
     9901 aatggctgat gactgagcct atcttctagt tcttttgccc tgcttgcaga tactgtcgca
     9961 gccttttctt tatctacttt ttttgaaaag cagggcatct tgttgatagc gtttcatctg
    10021 ataagcttac tttttaaaat cacggagctt agacagctat tgcttcactt ctttataagc
    10081 aacgatacta gaattcatgg atagcaagaa tcaattgtgt caaagactct taatagctag
    10141 tcataggcct gcagccaaat ctttttcaaa gcgtttagca atctcttcgt ctctgcaaat
    10201 cacaagcaag gtatccgcac cagcaatggt tcctagaata tcttcaatgg catcattgtc
    10261 aatcaaatta gctagaacat cagcctctcc taaattggta tggagaacca gcataaaacc
    10321 tgctcgatca actttcaaaa cataaaaacg cacctcagga ctaaatggtg ttgaaacggg
    10381 ttcagacaaa ctataataga gtttgccttg ttcatctctc aatttcaaaa ggccaatttc
    10441 tcttaagtcc cgagatagag ttgcttgggt gaccacaatg ccttcttttt gaaggtgatt
    10501 tttaatatct tcttgtgtgc caatatgctc tgcttgaatg attcgcttaa tctgctgttg
    10561 acgctccatt ttattcatga tatatcaccc caattgtata attatcctct caaattataa
    10621 caaaaaaagt gtattatttc atcttcgccc agctattttc agcaattttt tatcaaattc
    10681 aactagaatt tcacttgaaa actgttgaaa agaaacctca tttctctttt tataacattt
    10741 tcagaaaaac aacaacctca agcaattcat ttccttgaaa gtttcagtat aatttatcaa
    10801 tcctctcctc gtttatttta aagaaatatg ttagaatagg gtgacaaaca atttttagga
    10861 gacgctgtat ggatactaaa actctaatcg caagtgaaat agctaaagtt gttccagaat
    10921 tagaacaaga tgctattttt aacttgcttg aaaccccaaa aaattctgac atgggagacc
    10981 ttgcctttcc agcctttagt ttagcaaaag tccttcgcaa agcccctcaa atgattgcta
    11041 gcgagttagc tgaacagatt gatgagagcc aatttgaaaa agtcgtagct gtcggccctt
    11101 acatcaactt tttccttgat aaagctaaga tttcctcaca ggtccttgaa caagtgatta
    11161 cagcaggttc agactatgct cagcaagacg aaggtcaagg gcgaaatgtt gctattgaca
    11221 tgtcaagccc aaacatcgct aagccatttt caatcggtca cctccgctca acagttattg
    11281 gtgatagcct tgctaatatc tttgctaaaa tgggttatca acctgtaaaa atcaatcacc
    11341 ttggtgactg gggtaaacaa tttggtatgc tcattgttgc ctacaaaaaa tggggagacg
    11401 aagctgctgt tcaggcacat cctattgatg aactcttaaa actttacgtt cgtatcaatg
    11461 ctgaagctga aattgatcac accgttgatg aagaagctcg tggatggttc cgtaaattgg
    11521 aagatggcga caaagaagct actgaattat ggcaatggtt ccgtgatgaa agtttacttg
    11581 agttcaaccg actttatgac caattacatg tcaccttcga tagttacaac ggtgaggcct
    11641 tctataatga caaaatggat gaggttcttg acttattaga agctaaaaac cttcttgtag
    11701 aatccaaagg tgcccaagtt gttaaccttg aaaaatacgg cattgaacac cctgccttga
    11761 tcaaaaaatc agatggcgct actctttaca ttacccgtga cctagctgcc gcactttacc
    11821 gtaaacgcac ttacgacttt gctaaatccg tttatgttgt tggtaatgag caagctgctc
    11881 acttcaaaca gttaaaagca gtgctaaaag aaatgggtta cgattggtca gatgacatga
    11941 cacacgttgc ttttggactt gtcacaaaag gcggtgctaa attatcaaca cgtaaaggta
    12001 atgttattct tcttgaacct acagttgctg aagccattaa ccgcgctgct tctcaaattg
    12061 aagcgaaaaa tccaaatctt gctgataaag aagctgttgc acacgctgtt ggtgtcggtg
    12121 ccatcaagtt ttacgatctc aaaacagatc gtatgaatgg gtatgacttt gaccttgaag
    12181 ccatggtttc ctttgaagga gaaactggac cttatgttca atacgctcac gctcgtatcc
    12241 aatctattct acgtaaagct gactttacgc catctgcaac caccacttac agtttagctg
    12301 atgctgaaag ctgggaaatt atcaaactta tccaagattt cccacgcatt atcaagcgaa
    12361 catcagataa ctttgagcct tctattatgg ctaaatttgc tattaactta gcacaaagct
    12421 ttaacaaata ttatgctcac actcgcattc ttgacgacaa ctcagaacgt gataaccgct
    12481 tagctctctg ctatgcaact gctactgttc ttaaagaagc gcttcgctta ctcggtgttg
    12541 acgcaccaaa tgaaatgtaa gtaataacat cactaaggct gtcacattta tgacagtctt
    12601 agttttttat attataataa tagcacaata tgttacagga gaatgccatg ccatcagaaa
    12661 aagaaatatt agatgctctt agtaaagttt atagcgaaca agtaatacaa gcagatgatt
    12721 attttcgtca agcgatcttc gagctcgcca gtcaacttga aaaagagggg atgagctcac
    12781 tactagctac aaaaattgac tcacttatca atcaatacat cttaacacat caatttgatg
    12841 ctcctaaaag catatttgat cttagtcgct tggtaaaaac aaaagcttgc cattacaagg
    12901 gaactgctat atcagctatt atgcttggta gtttcttaag tgggggacca aaataaaaat
    12961 acaggctgtt tgacctgtat ttttatttat cataatttct tcggcgaatt ttaaaatcag
    13021 agctaataac ttcgtccaca tcaataatgg agataaaggc atctggatcc aaaccagcta
    13081 aaatacgttt gacttcccga acttcagttg ggttcaaggt cacatacatg atattgtaat
    13141 cattaccaga gtaagctcct tgacctttca agtaagtcac tccacgattg atttcggtta
    13201 ggatagcttg agcggcagcc tctgaatgct tggtaataat aatcattcca cggatagtgt
    13261 aaccaccatt ttgaaccaca ctaatcattt ggctaaaaac aaagcttgcc accaaagtat
    13321 aaagcatgtg ttttaaatcc acataagtca acgaagctgt caatacaaga gcatccacca
    13381 aaagcaaagt ttgacctagc ttagctccaa atttttcttc tgcaatgcga ccaataatat
    13441 ccgttccacc tgtggttgca ccatagcgaa aaacaatgcc actcccaatc cctgaaaaga
    13501 gacctgccac tacagccacc aacatcatgt cattttccaa gccaagttcg ataggaactt
    13561 tttgccacat ccagataaaa gcggacatca gaactgttcc atagatggtt agagccaaag
    13621 aacgcttccc aaaaatcttt gcccctaaaa taaaaagtgg aagattaaat aagagagaag
    13681 agtaagcagg attaatccca aaatgcgcgt gtaaaatcaa ggttattcct gccacaccac
    13741 cttcagccaa agcattagcc atgttaaaat taacaaaacc gaaggtataa atggctacac
    13801 ctagggcaat caaaaacagt tttagtagtt tagtcaattt atcgtgatgt gccatccttt
    13861 atcctcacta ttcttcgcag gcaactctaa tgctcattta tttcctcttt ttatggtatt
    13921 tgcttttaat agttaaaaat tctgggctat tgacttcatt aacatcaaaa taagtgataa
    13981 aggcattggg atcaacctga gttaaaaaag cttttaattc cctaatatct cttcgcccca
    14041 aagccacata aatcactttt ttacggcgcc cagaataggc tccttcacct gttagataag
    14101 taactcctct tcccaattcg tccatgatga accgggcaat tttctccgct tgttctgaaa
    14161 cgataaaaat gccacgggct gaataaccac cttgttgtac caagcgaaca atttgaccat
    14221 acataaagct agcaatcaga gcatacatca tttgcggcag ggaaatataa gtcaaagaga
    14281 caatcatgac aaaaagatca atgcctaaaa gagcttgatt cagctgtagt cctaacttgt
    14341 cttctaataa cttagcaata atatctgctc caccgatggt tccaccattg cgaaacacaa
    14401 gaccgccacc tagaccacca ataattccag ccatcaagga aacgactaaa ttgtcatggt
    14461 ccaagtcaac gaccaaagga aagcgttgaa acagccacac aaaaaagtaa agtgacaaaa
    14521 tacccattac tgtgtaaacc atggctcttt tcccaaaaca tctagctccc aacaagacca
    14581 aaggcaaatt aatcaaatag cctatcaaag aaggatcaat ctcaaaaagt gccttaccaa
    14641 ctagggtgag accagctaaa ccattggctg caatgcgatt agccatgtaa aaataaacaa
    14701 aagcaaaggc gtagatagca gctcctaaaa taataaagca aaatttcttt aactgtaaac
    14761 gttggtcaat tgacatgaca cctcctgtgt ttttcttaat catcttccac aaaacgaccg
    14821 ataattctaa cattctcagc cactgaaaca aaggcttgtg ggtctgtttt taacatcagc
    14881 catttgaatt ctgggtattc ttcacatgtc aaaatggtaa tcaaaactgc tttttgttca
    14941 tgcttgtaag tcccttctgc gtcgttgata ctggtcaccc cgcgatgcag acgtttatgg
    15001 atcatatgaa tcacacgctc tggatggctg gtaacaatag ttgcctgcat tttcttttgc
    15061 ttggtgaaaa tggcatccgt aacacgactt gatacaaaga tagagaccat agaataaagg
    15121 gcgtactgcc agccaaataa aatccctgca aaggctaaaa ttgcaccatt aaccatcaat
    15181 gagagactgc ccacatcctt gcctgttcgt tttctaaggg tcaaactgac aatatcagtc
    15241 cccccactag agatacgtga tttgagacct gtaccaattc ccattcccat aaccaaacca
    15301 ccaaaaatag cattgataag aggatcagtc gtcagcgtca cttgaggtat gatttgaata
    15361 aagaaagaac tcatgctgac tgtgataaag gtaaaaatgg taaattgatg cccaatttta
    15421 taccaagcta aaataagcaa aggaatatta atcaaataaa acgcaaaggc gatgggaaaa
    15481 tcatagccta aaagacgatg actaagagct gaaaaaacct gtgctagacc agttgctcca
    15541 cttgaataaa catgcccagg ctggaagaaa aaattcacag caatactaga gagaatgcca
    15601 taaagcagag aagccgaaat cttctctgca tatttttctc ttgaaatact tcttagagcg
    15661 tggagtaggc caactttctt ggcccctcta ctaatgacat atttaacttt tttcttatag
    15721 gttgttttct taatcatgac tttctatttg aagactgagt tcttctaatt ggttttcaga
    15781 aacaagacta ggtgcttgcg tcattggatc agaggctttg ttatttttag ggaaggcaat
    15841 aacttctcga atattgtctt ttccagcaag caacatgaca aaacggtcaa gaccaattgc
    15901 caagccacca tgaggaggaa agccgtagtc catcgcttct agtaagaaac caaattgggc
    15961 gttagcttca tcagcggtaa agccaagagc cttgaacatg cgctcttgca tctctttttg
    16021 attgatacgc aggctaccac caccaagctc gtaaccattt aaaacgatgt cgtaagcaat
    16081 agcccgtacc ttagccaaat caccctctaa ctcatgagca gattctggcg ttggaagagt
    16141 gaatgggtga tgagctgaca tataacgtcc ttcttcttct gaccactcaa acattggcca
    16201 atcaaccacc caaaggaaat taaactgcga ctgatcaatc atgtcaaggt ccttggcaat
    16261 acgattacga agggcaccta atgtattatt agcaacctca agagtatccg ctacaaataa
    16321 aaccaaatca ttctcagcaa gttttaattg gcttgacaac tctgtctcaa tagctgtcaa
    16381 gaacttggct actgggcctg ccaattgacc atttgtcacc ttaacccagg caaggccttt
    16441 agcaccaaat tgtttggcaa actcagttaa tttgtcaata tctttacgag agtatcgatc
    16501 tgcatttccc ttaaccacaa tcgctttgac agctggtgct tctgagaaaa ccttgaagtc
    16561 aattcccttg acagttactg tcaagtcttg taaaagcatc tcaaaacgtg tgtctggttt
    16621 atcagagcca tagctattca ttgctacatc gtagctcata cgtgggaatg gtagggttac
    16681 atctatttct tttgtctctt tcatcacttt agcaatcatg ccttcaacga tgtcttgaat
    16741 ttcttgttca gaaaggaatg acgtttccaa gtcaacttgt gtaaactcag gttgacggtc
    16801 accacgtaga tcttcatcgc ggaaacattt cacaatttga taatagcgat caaagccagc
    16861 attcatcaat aactgtttgg taatttgcgg actttgtggc aaagcataga aatgcccttg
    16921 gctaacacga cttggtacca gatagtcacg cgcaccttct ggagttgatt ttgtcaacat
    16981 aggtgtctcg acatcaataa actcaaggtc atcaaggtaa ttacggatag aatgagtcac
    17041 ttttgcacgt agcttgaagt tttctaacat ttctggacgt cgtaaatcta agtaacggta
    17101 acgtaaacgc gtgtcatcac ttacttcaac gtcatcttta atttcaaaag gcgtcgtttt
    17161 agctgtgttt aaaatggtca aagcagatac ttttaactca accattcctg tcgccaactt
    17221 atcattggct tgttgtcgag cttcaacaaa accttccact tcaatcacat attcactacg
    17281 taggcgttca gcagttgcca tcacatcact ggaaacttcc tcagggttaa tgactaattg
    17341 catcacaccc tcacggtcac gtaaatcaat aaagatgaga ccacccaagt cacgacgacg
    17401 actgacccat cctttcaatg tgattgtcgt tccaatgtgt tcttcgcgaa cacgccctgc
    17461 atacatgcta cgtttcataa ataaattccc taaaacaaac agaatcctgt gaaacctgct
    17521 cgtttccaat tcctttcact atataaatca attaattcat tccctactat tatagcaaaa
    17581 agcccttaaa aaccccaccc atttaaaaag agtttttcta aagataaagc aagaatactg
    17641 agtcgtcagc attttgacat atcaggtgtc ctcacagaat actaagaaag ctaatcaatc
    17701 cccttacaga aaacaaacag gtgcaactcc cttctaaaaa gagaagttac acctgtttta
    17761 gtgattatct tctaaacaat catatcaaca caagcttatg acgacaataa aatcattaag
    17821 acaataattg ctctgagatg ttttcaaagt tggtcatcat atcttcaaaa ctgacttcca
    17881 cttcttgacg gctacgatta ttcttaatca cagctttgcc tgcttcaact tcactttctc
    17941 ctaaagtcat taccaatttt gctttaaagg tatcagctga tttaaactga gccttaatct
    18001 tacggccaag gtaatctcgt tcagctgtaa acccttgacg gcgaatggct tgaaccaatt
    18061 ctaaagcttt gctattagca ccatcaccta acacagcaag gtaaatatcc atctctgtct
    18121 caattggcaa ggtaatgccc tgcttttcaa taatcatcaa gagacgttct aaaccaagac
    18181 caaagccaaa tcctggagtc tcaggcccac caaaataacc tactaaacta tcataacgtc
    18241 cacccgcaca gatagttaag tcagaacctt ccacagacgt gatgaattca aaaatagtgt
    18301 ggttatagta atcaaggcca cgcaccatat tggtgtcaat cacataagga atgtcaagtg
    18361 cttcaagcat atctttgacc gcctcaaagt gcgcctgact ttcctcatct aagtaatcca
    18421 aaatagaagg cgctttttca acggctagtt tatcttcttt ttctttagaa tcaagcacgc
    18481 gcagtgggtt ttcatccaag cgacgttgac tatctttaga taattgatca cgcattggtg
    18541 ttaagtagtc aatcaaggct tgacgataag ctgcacgact ctcaggactc cccaaactat
    18601 ttaaatgaag ggtgacatcc ttaatgccca atttttcaaa gaggtgatag gccatcgcaa
    18661 tggtttcaac atctgttgct ggattagcag cgccgaaaca ttctacccca atctggtgaa
    18721 attcacgtaa acgaccagct tgaggacgtt cgtagcggaa catggaaccg atataataaa
    18781 gtttgacggg tttttggacc tctggcgcaa acaatttatt ttcaacataa gaacgcacca
    18841 ctggagctgt accctctggt cgcaaggtaa tgtgacgatc tcctttatca tagaaatcat
    18901 acatttcctt cgtcacaata tcagtcgtat cacccacaga ccgcgagatt acctcgtagt
    18961 gttcaaacat aggagtacga atttctccat aattgtattg gctaaacgta tcacgcgcaa
    19021 cagattccac atactgccat ttcgcagcat ccccaggcag aatatcctga gtcccttttg
    19081 gtttttgaag tttcataagt ttacctcttt gattagtttt ctctttaaac attctaccat
    19141 tttttcttat attttaaaca agcatatatc atttgcttta gtttgatagt attttcacca
    19201 tgaaattgta aaaataaaaa gttttgtcaa gtaactttac tttacaaaaa agatatgtta
    19261 tcatagtgaa gttgatgaaa atcaaaactt acacatcgaa aggaagaaca agaaaatggc
    19321 agtacctgca cgtcacacgt caaaagcgaa gaaaaacaaa cgtcgtacac actacaaatt
    19381 gacagctcca tctgtacaat tcgacgaaac tactggagat tactcacgtt ctcaccgtgt
    19441 atcccttaaa ggatactaca aaggacgtaa aatcgctaaa gctaacgaag ctaaataata
    19501 gaagggagat accatgcgcg taaatattac acttgaacat aaagaatctg gtgaacgctt
    19561 gtaccttact tcaaaaaaca aacgtaacac tccagaccgt cttcaattga aaaaatactc
    19621 accaaaatta cgtaaacacg ttacatttac agaagttaag taaggggttc actacacgtc
    19681 attagacgtc aaaacattga ttttaagcga tttttgaatt ttttgaagtt cattacattg
    19741 caatataaat caataaaaac tctacctttt actctacctt ttttagatga atctatcatt
    19801 tggatattga agttaggccc tgttatcatt tcgatggcag ggctttttaa tatttggtac
    19861 actaactcct tatctatatt ttggtggagc tgaagttgac atctataaag tttaatgtta
    19921 atatacattc aaaaatacat ttgaatgagg tgttttatga ttcaaaatgt tgttacttca
    19981 ataatcctgt attctgggac agccgtagac ttacttatta tcctaatgtt attttttgcc
    20041 aaaagaaaaa gcagaaaaga catcattaac atctatttag gacaatttct aggctctgtt
    20101 agtctaatat tgctaagttt gctttttgca tttgtcttag attatattcc tagtaaagag
    20161 attttaggtt tgctcggttt gattccaatt ttcctaggcc tcaaagtttt gcttttagga
    20221 gattctgatg gagaagctat tgccaaagaa ggtttgcgca aagataataa aaacctgatt
    20281 tttctagtcg ctatgattac ttttgcaagt tgtggtgctg acaatattgg tgtctttgtc
    20341 ccgtatttta ctaccttaaa tttagcgaat ttgatagtgg ctttacttac ctttctagtc
    20401 atgatttatc tcttggtttt ttctgcccaa aaattggcac aagtcccttc tgttggagaa
    20461 attttggaaa aatatagcag atggtttatt gccgttgttt atttaggatt ggggatatat
    20521 atcctgattg aaaacaacag ttttgacatg ctatgggctg tgttaggcta ggagaaaata
    20581 ttatgaaaaa agatagtatc tgccaagtga atgttataaa tcaacaaaat gttacaaccg
    20641 caacgaacta ccttgaaaag gaaaaagtcc aaaaatcact tcgtatttta tcaaagttta
    20701 ccgataataa acagataaat atcatctttt atctccttac tgttgaagaa ctctgtgtct
    20761 gcgatatagc ctgtttacta aatctcagta tggcatctgc ctcccaccat cttcgtaaac
    20821 tagccaatca aaacatcttg gacactagaa gagaggggaa aattatatat tattttataa
    20881 aagatgagga aatcagagat ttttttaatc aactaggata acaactattt ttaccacttt
    20941 tccatgttta tagagggctt atctatgaaa ttttttgatg aaaattactc acaagaaata
    21001 cctactcgta tcaaatgttt aagaaaaaag tataatttaa agtaaagtga cctaggtaat
    21061 gcaggtcaag ttagccaagt tgaaaaggga aagcgacaag ttaccgcttc aatattgctt
    21121 tatttgaata ctcaaaccga ctcggactat aaagaaatta tttttggaga tattgccaag
    21181 tttgttgaaa atatgtttta ccactacttc agctctattt tatttagaga tttggaaact
    21241 gtagataaaa gaatgtattc tttttcggac gatgacttaa tttcaataca atccagctgt
    21301 cttagacttt ccaagacatt cgcaaatttc aatattcaaa gaaaaaattt tttggcttct
    21361 gatgaaactg aaatggatac ttttcacaag aaagatgaca ttgacattac tgttggtgaa
    21421 aaaagttata acctcgctag aagttttaga accagtacca tcaatgaatt gactgtcttt
    21481 aattttgaag aaatgtttga tattatttgg ttgatgttag gggataattt aattaaatca
    21541 tttgaagtta atgtctgtgg tatcctattt gaattagacg gaaatggcat accctcaaca
    21601 tttaggcaag aaaacattga tcccctcatc aataaatggt ggtatgataa tgtttcgaca
    21661 gaaattatcc ctaacctaat aaaaaaactc aaagaaaatc cactatttaa tatcggattc
    21721 ctggttgatg atattctaga acgaatgtat aaagaaaata tcccaaaatc ttaccttacg
    21781 tccgtcccgt tagttatttc caaaaaagca aggtcaactt tatcgattaa gtcaaacgga
    21841 agtcaaaaaa tagataaatc aaaatttgca cagattttgt ccctttagag acggatgact
    21901 tacacattca acgtggatta ttcttccctt ggagaaaggg gatttcagaa cgcttggcga
    21961 ttcctgattt agataaattt tcatctagct tacaacaggg gaaaattcct gtcataaagc
    22021 acttcgaact caatttagat aaggggagtc actgggctat tgctggaaat tcaggttctg
    22081 ggaaaccgta cgctctcact tacttcttga gtgtcctgaa acctatgtcc ggcctaatta
    22141 tcattgaccc aaaattcgat acccctagcc aatgggcaag agaaaataaa attgccgtta
    22201 tccacccagt agaaaaccat tcaaaatctg atttcgtttc acaagtcaat gaacaattaa
    22261 accagtgtgc aactctcatt cagaaacgac aagctatctt gtatgataat cccaatcatc
    22321 aatttaccca tctaactatt gttattgatg aagtcctcgc tctatcagag ggagtcaata
    22381 agaatatcaa agaagctttt ttctccctac tatcacaaat tgccttgttg ggacatgcta
    22441 ctaaaatcca tctattcttg ggaagccagc gttttgacca taacactatt cccatttcag
    22501 tgagggaaca gctaaacgta ttattgcaaa ttggaaatat caatcagaaa accactcaat
    22561 tcttatttcc agacctagat ccagagggga ttgtcattcc aacaggacac ggaacaggta
    22621 tcattcaagt tgtcgataat gaacactcct atcaagttct acccttactc tgtccaacct
    22681 actatactaa gcgaggtatc ctatgaccac taaaacctac ttcatgagaa gcttcaatct
    22741 catattatac ttactgctta ttgctccaat ctgctatgtg ttcggttggc tgttcaatct
    22801 tgtttcagtt caattatcta ccgtcacatt tgtcaatctg gatacggtta aaaacgttac
    22861 tgataccatt agttctgtat gttatggatt cgctcttatt tctttatttc tagccttatt
    22921 atctggatta aagatgatac aacgcttgaa tacagatagc cttgggaact actgccaatc
    22981 tatttatcgc acctttctac taagacactt ccttttccag cgtgaacgag tacagaaggt
    23041 aagcaacttt gaacaccaga ccgttaccac tatcaatcca atccataacg ggtttaaccg
    23101 agctgtacgt aagtgtatcg tagatatacg aaaagatgcc attactgtat ttctaaaagt
    23161 ttctagagac ccaacagggg caaaaaatcc taaaggatac gggagtacaa ctgaaagaag
    23221 agattgtcat ccaacacaca gactattatt tctcttctcc tgttcgagtt cgtaaccaat
    23281 tgtggtttat tggtcaaaag cgttaattcc ttggggctgt ggcttgcgtt aagcaaaagc
    23341 cagacagccc cttttttata ttcttatcta tcatgaccta ttggggttac tacgacaacg
    23401 aaagttagaa gctgtgggta tttatttatc ttgaaaagat tgctgaacta acgcaataac
    23461 tactctacca ttcactctac cttttattaa gcaactatca ataaatattg atacaataag
    23521 gtttataaca gctaaaatag aaaatactta acctttacag aagtaaaata attgagatga
    23581 gaaaagccta tgaaatgaac gtttcaaagg cttttttcgt gttaaaaaaa acaaatgaaa
    23641 aaacaaaata gagagtaggg ttattaacac tgtcaatcct gtgtacgcaa taacaatcca
    23701 tgctttttga taaggatatt ttgctttttc tttaatctta tcaatctgat ctaagctctc
    23761 accctttgct atagcttcaa gttatcggta agtctgaaca tcatatgtct ttttaaatcg
    23821 ctcaattctt aaggcgctac gcatgattat caaccataag atagcaaccg gaccatatcc
    23881 ccaccaacct aaccaagaca ttataggata aatactgata gcaattagga gtatcccaaa
    23941 taacataata tgcgtattcc tatcacattg tttaatctcc tcatccttaa taattaaagt
    24001 catttttggg atatcttctg ctattagttg ctctaaagga acctgtaagg acttacttaa
    24061 catcaaaaga ttttgcaggt ctggatagct tttaccgttt tcccagtttg agatagtctt
    24121 cttgaaacat acatcatttc agcaagctct tcttgtgaca tctgtaattt ttttcaataa
    24181 taaggtaact ggtttttaaa gcccataata aatctccttt ttaatagcta ctataatcct
    24241 tcttcaataa gtttactatc aaaagtcttt gacctaagct ttttttcgtt tatctttttg
    24301 ataattatcg taaggtttac agtctttgta aattttattt acaactttga caatcaaact
    24361 tgacttgatc acaaagacat ggtgtttaag
//
LOCUS       DATURV010000022        23770 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DATURV010000022 DATURV010000000
VERSION     DATURV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23770
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.022"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            15..2729
                     /locus_tag="VCF75_000860"
     CDS             15..2729
                     /locus_tag="VCF75_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEP2546437.1"
                     /translation="MIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKRESVISD
                     KSNFPVISPYLASVDFGERKTSLPTPDKGVKVTTEQSIAQVRKGPEERTYTVTGKITS
                     VINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQQVTAHK
                     KLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDDSGKSIV
                     VHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKKVTSSNS
                     DASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDGDLATSD
                     GIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVIKTGTAQVP
                     SPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGPMKNKEI
                     YVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAGPVSYSY
                     GNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSSTKDEKVK
                     RIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGPTYRYVD
                     IAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSVGRIDPT
                     NAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGCVQPVTFKSEQRRHVLAN
                     MLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRYSYFHQG
                     NNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKENDKAESS
                     KQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      2894..3009
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            3007..3507
                     /locus_tag="VCF75_000861"
     CDS             3007..3507
                     /locus_tag="VCF75_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEP2546438.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            3701..5659
                     /gene="ligA"
                     /locus_tag="VCF75_000862"
     CDS             3701..5659
                     /gene="ligA"
                     /locus_tag="VCF75_000862"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEP2546439.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWNFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKEDFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRKL"
     gene            5673..6695
                     /locus_tag="VCF75_000863"
     CDS             5673..6695
                     /locus_tag="VCF75_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP2546440.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            7087..7284
                     /locus_tag="VCF75_000864"
     CDS             7087..7284
                     /locus_tag="VCF75_000864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEP2546441.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            7319..8035
                     /gene="atpB"
                     /locus_tag="VCF75_000865"
     CDS             7319..8035
                     /gene="atpB"
                     /locus_tag="VCF75_000865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEP2546442.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            8053..8547
                     /gene="atpF"
                     /locus_tag="VCF75_000866"
     CDS             8053..8547
                     /gene="atpF"
                     /locus_tag="VCF75_000866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEP2546443.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            8547..9083
                     /locus_tag="VCF75_000867"
     CDS             8547..9083
                     /locus_tag="VCF75_000867"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEP2546444.1"
                     /translation="MTKKEQAIIEQYAKSLVEVASEHHSLDTLQADVLAILETFETTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKNRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            9099..10607
                     /locus_tag="VCF75_000868"
     CDS             9099..10607
                     /locus_tag="VCF75_000868"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEP2546445.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            10623..11498
                     /locus_tag="VCF75_000869"
     CDS             10623..11498
                     /locus_tag="VCF75_000869"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEP2546446.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            11660..13066
                     /gene="atpD"
                     /locus_tag="VCF75_000870"
     CDS             11660..13066
                     /gene="atpD"
                     /locus_tag="VCF75_000870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEP2546447.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDHLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            13079..13495
                     /locus_tag="VCF75_000871"
     CDS             13079..13495
                     /locus_tag="VCF75_000871"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEP2546448.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            13761..14018
                     /locus_tag="VCF75_000872"
     CDS             13761..14018
                     /locus_tag="VCF75_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEP2546449.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            14083..15354
                     /gene="murA"
                     /locus_tag="VCF75_000873"
     CDS             14083..15354
                     /gene="murA"
                     /locus_tag="VCF75_000873"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP2546450.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTHLRGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            15358..15546
                     /locus_tag="VCF75_000874"
     CDS             15358..15546
                     /locus_tag="VCF75_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP2546451.1"
                     /translation="MTNGLKYVLEQMLLLFIIAALACLFLAIGLMIGYSFMGDGQSPW
                     HILSMDKWAELVNKFTGK"
     gene            15582..16121
                     /locus_tag="VCF75_000875"
     CDS             15582..16121
                     /locus_tag="VCF75_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEP2546452.1"
                     /translation="MLTRKKRKRVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRTFK
                     PAGWHRLHHLKGSYDHAVDRGHLLGYALVGGLKGFDASTGNPDNIATQLSWANQANKP
                     YSTGQNYYEGLVCRALDKGHRVRYRVTLLYDGDNLLASGSHLEAKSSDDSLTFNVFVP
                     NVQAGLTVDYRTGQIAINL"
     gene            16404..17447
                     /gene="pheS"
                     /locus_tag="VCF75_000876"
     CDS             16404..17447
                     /gene="pheS"
                     /locus_tag="VCF75_000876"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEP2546453.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLEMIIKKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            17657..20062
                     /locus_tag="VCF75_000877"
     CDS             17657..20062
                     /locus_tag="VCF75_000877"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEP2546454.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEVAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEVGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKENLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLASMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELNMAALEAALSSDQTFVEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLTEQVGA
                     EVR"
     gene            20173..20550
                     /locus_tag="VCF75_000878"
     CDS             20173..20550
                     /locus_tag="VCF75_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEP2546455.1"
                     /translation="MYQTKITGNRLYRTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            20543..20929
                     /locus_tag="VCF75_000879"
     CDS             20543..20929
                     /locus_tag="VCF75_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEP2546456.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            21002..22078
                     /locus_tag="VCF75_000880"
     CDS             21002..22078
                     /locus_tag="VCF75_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546457.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSVLLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GIGNITIAQAVLLQTFLLSALGSGLGLLGTWLTSLVLPEAVPFQSNWLVYLAILVSMV
                     CFALLGTLFSVFSIVRIDPLKAIG"
     gene            22088..22756
                     /locus_tag="VCF75_000881"
     CDS             22088..22756
                     /locus_tag="VCF75_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546458.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGITGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYQMQDGEL
                     RQER"
     gene            complement(22860..>23770)
                     /locus_tag="VCF75_000882"
     CDS             complement(22860..>23770)
                     /locus_tag="VCF75_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEP2546459.1"
                     /translation="DDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWKTKL
                     VILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVFAST
                     EDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISFYNE
                     LSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLELQA
                     GYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRAVPDSFTHGTSKQR
                     QRWFDRGYQYGDFEHGDTFSIPYSKL"
BASE COUNT         7250 a         4196 c         5162 g         7162 t
ORIGIN      
        1 ttaacaagaa atatataata cctgtttcat tgttgacact agctattacg cttactagtg
       61 ttgaagaagt tacttcacgc caaaatttga cttatgccaa tgaaatcgta acacaaaggc
      121 caaagagaga atctgttatt agtgataaat cgaattttcc cgtcatatca ccttacctag
      181 caagtgtgga ttttggtgag agaaaaacat ctttgccaac acctgataaa ggagtaaaag
      241 taactactga acagtctatt gctcaagtaa gaaaggggcc tgaagaaaga acctatactg
      301 ttactggcaa gattacgagt gtgatcaatg gctggggagg ctatggcttt tatattcaag
      361 atagtgaagg tattggactt tatgtttatc ctcaaaaaga tttaggatac agtaagggag
      421 atattgttca attaacaggt acacttactc gctttaaagg tgatttacaa ctccaacagg
      481 tgactgcaca caaaaagtta gagttatctt ttccgacttc tgttaaagaa gcagtaatat
      541 cagaattaga aacaacaaca ccctcaacat tagttaagtt atctcacgtg acagttggag
      601 aattatcaac tgatcaatat aacaacacat ctttccttgt aagggatgat agtggtaaaa
      661 gtatagttgt tcatatagat catcgtacag gggttaaagg ggctgatgtt gttactaaaa
      721 taagtcaggg tgatttgatt aacctcacag ccatattgtc tattgttgat ggtcagttac
      781 aattaagacc gttttctctt gaacaattgg aagtggttaa aaaggtcaca agctcaaata
      841 gtgatgcttc atctcgtaat attgtgaaaa taggcgagat tcaaggagct agtcatacgt
      901 cgccacttct caaaaaagcg gtcaccgtag aacaggttgt tgtcacttat ttagacgatt
      961 ccactcattt ttatgttcaa gatcttaatg gtgatggtga tttagcgact tcagatggta
     1021 ttcgtgtttt tgctaaaaac gctaaggttc aagtcggcga tgttttgacc atttcaggtg
     1081 aagtggaaga attctttggt cgtggttatg aggaacgtaa gcagactgac cttaccatca
     1141 cccaaattgt ggctaaagca gtgatcaaaa cagggacagc tcaagttcca tcaccgcttg
     1201 ttttagggaa agatcgtatc gcgccagcca atattattga taatgatggc ttgcgtgtgt
     1261 ttgatccaga agaagacgct attgattatt gggaatcaat ggaaggcatg ttagtggcgg
     1321 ttgatgatgc taaaatcctt ggtccaatga aaaataaaga aatttatgtc ttacctggct
     1381 ctagtacaag accgttaaat aattcaggtg gagtattact tccagctaat tcttataaca
     1441 cagatgtgat tcctgttctt ttcaaaaaag gcaaacaaat tattaaagca ggagactctt
     1501 acaaaggaag attagctggg ccagtatctt atagctatgg taattacaag gtctttgttg
     1561 atgacagcaa aaacatgcca agtttaatgg atggtcatct aaaacctgaa aaaacaaact
     1621 tgcaaaaaga ccttagcaag ttaagcattg cttcttacaa tattgaaaac ttctcagcca
     1681 atccttcttc aactaaagat gagaaggtca aacggattgc cgaatccttt attcatgatc
     1741 tgaatgctcc agacattatt ggattaattg aagtccaaga taataatggg ccgactgatg
     1801 atgggacaac ggatgcgaca caaagcgcac aacgcctcat tgatgctatt aaaaaactag
     1861 gtggcccaac ttatcgttat gttgatattg ctccagaaaa taatgttgac ggaggtcaac
     1921 caggtggtaa tattcgaaca ggattccttt atcaaccaga gcgcgtcagc ctttctgata
     1981 agccaaaagg cggtgctcgt gatgctctaa cttgggttaa tggagaatta aaccttagtg
     2041 ttggtcgaat tgatccaact aacgccgctt ggaaagatgt tcgtaaatca ctagcagcag
     2101 aatttatctt ccaaggtcgt aaagtcgttg ttgttgcaaa tcatttgaac tctaagcgtg
     2161 gggataatgc tctttatggt tgtgtgcaac cagtcacttt taaatctgag caaagacgtc
     2221 acgtcttggc taatatgcta gcacaatttg cgaaagaagg ggcaaaacac caagctaata
     2281 ttgtgatgct aggtgacttt aatgattttg aattcacaaa gacgattcaa ttaatcgaag
     2341 aaggtgatat ggttaacttg gtgagccgac atgatatttc agatcggtat tcttattttc
     2401 accaaggcaa taatcagacc cttgataata tattagtttc acgccattta cttgatcact
     2461 acgaatttga catggttcat gtgaattccc catttatgga agctcacgga cgcgcatcag
     2521 atcatgatcc attgttactt caattatcat tttccaaaga aaatgataag gcagagtctt
     2581 ctaaacaaag tgtaaaagct aaaaaaactt caaaaggaaa actgttgcca aaaacaggag
     2641 atagtcttgt ttatgtgata acgctactag gaacggctag tttattagtg cctattttat
     2701 tattgactaa aggcaaaaag gaatcatagc gatttgaaga attggagttc ataaagagtc
     2761 gttaaaggct tgatatactt atgttagacc ttaagctaaa atggatgact atataaaaac
     2821 taatattgat aacaaaagaa gataacaata agacagcttt acttgctaaa gacttttgtc
     2881 aagctaattg ttttatggta caatgtaacc gttgctttgc aacccttggt gcttagttcc
     2941 ctttcaccaa gcatattaga aacgtagata gcttttgaga gttatccaga cgttaaagga
     3001 gaaaaaatga caaaattgac tgtacacgac tatgttcaca ttgggctagt agctgccttg
     3061 tatgttgtct taactattac cccgcctcta aatgcgattt cttatgggat gtatcaattt
     3121 agaatatctg aaatgatgaa tttcttagct ttttatcatc gcaagtatat tattgcggtg
     3181 actcttggat gtatgattgc taacttttat agtttcggac tgattgatgt ctttgtaggc
     3241 ggcggttcaa ctcttatttt tgtgacctta ggtgttattt tgtttagtaa gtatcaaaaa
     3301 gattatcttt ttaatggcat ttttaataaa gcttttgttt acttttcttt cttttttgca
     3361 acttccatgt ttactgtagc aatcgaactt tacttctttg gagctccatt tttattaaca
     3421 tggtttacaa ctgcgttggg agaattagta tctctgctca taggatcact tattattgat
     3481 aagttgtctc aaagaatttc cttttagtaa tgcatcatac tcaaaagagt tgggtcaata
     3541 cccttctctt ttttatgttg tttgtaatgt taaaatcgtt ctagatagaa atatttaaca
     3601 ctttagctaa tagtaccata tggagtggcc aacgattact tttgatgttg ttatgatata
     3661 gaaatagctc atttgttatc ttttatgata aaataaaagt atgaaaaaac gcataaaaga
     3721 attaacagat cttcttaacc gttaccgtta tgactattat actaaagatg ccccatcagt
     3781 ttctgacagt gactatgata agctttatcg tgaactggtg actttagagc agtcatatcc
     3841 cgagtatgtc ttgcaagata gcccaacgca gcaggttgga gggactattt taaaggggtt
     3901 tgaaaaatat cgacatcagt atccgctttt tagtttgcag gatgcttttt cacgtgagga
     3961 gctggatgct tttgataagc gcgtcaaggc cgaatttccg aatgccacct atctagcaga
     4021 gttaaaaatt gatggtttat ctatttcctt gagttatgag aatggcttct tacaggttgg
     4081 agcaacacgg ggtgatggca atatcggtga aaatatcaca gaaaatatta aaaaaataaa
     4141 ggatattcct caccaattga gtgagccttt aaccatcacc gttcgaggag aggcttacat
     4201 gtctcgtcag tcctttaaag cgatcaatga agcacgccaa gaaaatgggg agactgaatt
     4261 tgctaatcct agaaatgctg ccgctgggac actaaggcag ctagatacgg cagtggttgc
     4321 taagcgtcaa ctagccactt ttttgtatca agaagctagt ccgacagcta gaaatcaaca
     4381 aaatgaggtc ttagcagaat tagcagatct aggtttttca gtaaatccgt attatcaact
     4441 cacatcgtct atggatgaga tttggaattt tatcaaaact attgaggcaa aaagagatca
     4501 attagcttat gacatagatg gcgttgttat taaagttaat agcctagcca tgcaagaaga
     4561 gctagggttt acggttaaag ctccacgctg ggctattgct tacaaatttc cagcagaaga
     4621 aaaagaggca gaaatccttt cggttgactg gacagtggga cgaacaggcg tggtcacacc
     4681 tacagcgaat ctaactcctg tgcaactagc ggggacaacc gtgagtcgtg caaccctaca
     4741 taatgtggat tatattgccg aaaaagatat ccgtattggc gatacagtga ttgtatataa
     4801 ggcaggggac attattcctg ctgtcttaaa tgtcgttatg agtaagcgga accagcaaga
     4861 agtcatgctt attcctaaac tatgcccatc ttgtggtagt gaattagttc attttgaaga
     4921 tgaggttgct ttgcgttgta ttaatccgtt gtgtcctagt ttgattcagc gaagtttgga
     4981 gcattttgca agtcgtgatg ccatgaacat tactgggctg ggaccagcta ttgtggaaaa
     5041 actattttta gcggggtttg ttcatgatgt tgctgatatt taccagttga ccaaagaaga
     5101 tttcatgcaa ttagatggca tcaaagaaaa gtcagctgat aaattgctcg ctgcgattga
     5161 ggcttctaaa tcaaattcag ctgaaaaact gttatttggt ctgggaattc gtcatattgg
     5221 ctctaaggtt agtcgcttaa ttttagaggt atacggagat atttctgctc ttttaaccgc
     5281 taaagaagag gaaatcgcaa ggatagatgg tcttggctca acgattgctc aatcacttac
     5341 tcaatacttt gaacaaaaaa cggctgcgat tttagtagac gagttaaaga cagcaggtgt
     5401 caacatgcat tactcaggtc aaaaagttaa tagtgacgca gcgctttttg gtctaactgt
     5461 tgttttaact ggcaaattaa atcagctgaa ccgaaatgag gccaaagata aattagaggc
     5521 attaggtgcc aaagttactg gaagtgtatc taagaaaaca gatttagtta ttgcaggtag
     5581 tgacgctggt tctaagctag aaaaagcaaa gagcttagga attcggattg aagatgaaga
     5641 ttggctacga aagctttaag tgaggtaatg ttatgaaaaa acaattgaga gcacgtctca
     5701 tttataatcc aacgtcagga caagaactta tgagaaaaag tgttcctgaa gttttagaca
     5761 tattagaagg gttcgggtat gaaacatctg cctttcaaac aacagcccaa aagaactctg
     5821 ctctaaatga agcaagaaga gcggcaaagg caggttttga cttattgatt gctgctggag
     5881 gtgatggcac tattaacgaa gtggtcaatg gaattgctcc cttgaaaaag cgccctaaaa
     5941 tggccattat tccaacaggt accactaatg attttgctag agcgttaaaa gtaccaagag
     6001 gaaatccgag ccaagcagca aaacttatcg gaaaaaatca gactatccag atggatattg
     6061 gtagagctaa gaaagatact tatttcatca atattgctgc agcaggaagt ttgacggaat
     6121 taacttacag tgtaccaagt cagttaaaaa cgatgtttgg atacttggct tatcttgcta
     6181 aaggagtaga actgctccca agagttagca atgtgcctgt taaaattacg catgacaaag
     6241 gcgtctttga aggtcaagtt tctatgattt ttgcagctat taccaattct gttgggggat
     6301 ttgaaatgat tgctcctgat gcaaaactag atgacggtat gtttacgctc attttaataa
     6361 agacagcaaa tctttttgaa attgtacatc ttttgcgttt gattttggat ggtggtaagc
     6421 atattacaga tcgtcgtgtc gaatacatta agaccagtaa aatagtcatt gaaccccaat
     6481 gtggtaagcg aatgatgatt aatttagatg gcgagtacgg aggagatgct ccaattacat
     6541 tagagaacct aaaaaatcat atcacttttt ttgcagatac tgatttgatt tctgatgacg
     6601 ctttagtctt agatcaggac gaattggaaa ttgaggaaat cgtaaagaaa tttgctcatg
     6661 aagtagaaga cttagaacaa gaattagaag aataatacaa tagttattag ctactgacag
     6721 tttagtcaaa tgcactacta ttagaatcta agatttttaa atcaattgag gtagtgtttt
     6781 ttgatgtgct caaagagtca atttattcat atgaggggcc tatttgaggt ctctcttttt
     6841 tgatcatatg caagaaaatg ccatgtttta ggggtgttat tatacccttt ttggaatctt
     6901 gttgcctttt tgttataata agagtgtaat tctattttta aaaagatctt aacaaagaca
     6961 gactcgttta tcaaaaaggt tagtattagt ctaagaaagc gtactcagtc taaagaaatc
     7021 tgttgacatt cttaatttta aggaatagaa tagcaaagta aaaaaaattt aaaggagtat
     7081 tctactatga atcctatttt cgcattggct ttggcctgtt ttggtgtttc tcttgccgaa
     7141 ggtttcttga tggcaaatct ttttaaagca gcatctcgcc aaccagaaat cattggtcaa
     7201 ttacgttcat taatgatctt gggtgttgcc tttatcgaag gtactttctt tgttaccctt
     7261 gttatggcct tcattcttaa ataacagcct atttgataat agaaaaggag ggtgttagat
     7321 ggaagaagct aaaataccca tgctaaagct tggtccaata acctttaatt tgaccctact
     7381 tgctgtttgt attgtcacaa ttgctgttat ctttgccttt gttttttggg caagtcgcca
     7441 aatgaaattg aaaccagaag ggaagcaaac tgctttagaa tatttaatca gttttgtgga
     7501 tggtattgga gaagagcact tagatcataa tctacaaaaa tcttattcgc tgttactctt
     7561 taccattttt ctctttgtgg ctgtcgctaa taatttaggg ttatttacta agttagagac
     7621 agttaatggc tataacctat ggacctcgcc aacagccaat ctggcttttg accttgctct
     7681 atctcttttt attaccttaa tggtacacat tgaaggggtt agacgtcgtg gcttggttgc
     7741 ccatttgaaa cgtttggcta caccgtggcc aatgactccg atgaatttat tagaagagtt
     7801 cacaaatttc ttatcacttg ccattaggtt attcggtaat atctttgccg gggaagttgt
     7861 tacaggtttg attgttcaac tggccaatta tcgagtttat tggtggccga tcgctttcct
     7921 agtcaatatg gcgtggacag ccttttcagt ctttatttcc tgcatacagg cttttgtttt
     7981 cacaaaacta acagcgacct atctaggaaa gaaagttaat gaatcagaag aataaggaag
     8041 gagaacaaac gaatgtcaat cacatttggt gaactcgttg ggaattttat tctagtaaca
     8101 ggttctgtaa tcgtcttgtt actcttgatc aaaaaattcg cttggggtgc aattgaatcg
     8161 attttacaga cacgctcaca acaaatctct cgagatattg atcaggctga gcaatcacgt
     8221 ctaagtgctc aacagttaga ggcaaaaagt caagctaacc tagatgctag tcgttcacaa
     8281 gcaagtaaaa tcattagtga tgccaaagaa attggtcaat tacaaggtga taaattggtg
     8341 gcagaagcta ctgatgaagc aaaacgcttg aaagaaaaag cgttgacaga tattgaacaa
     8401 agcaaatcag acgctatctc agcagtcaaa acagaaatgt ctgatttaac ggttctttta
     8461 gcggaaaaaa ttatgggagc caatcttgat aagacggcgc aaagccagct tattgacagt
     8521 tatcttgatg acttaggaga agcttaatga ccaaaaaaga acaggctatt atcgagcaat
     8581 atgcaaaaag ccttgttgag gtagcaagtg aacatcattc tcttgatacc ttacaagctg
     8641 atgtgcttgc gattttagag acgtttgaaa caactaatct tgatcaaagt ctatcaagtc
     8701 tagctgttcc gcatgctgaa aaaataaaat tgctaacctt attaaaggga aataattctg
     8761 tttatatgaa taatttcttg aatctcattt tacaaaacga acgtgaagct tatttgtatc
     8821 aaatgcttca ggctgtttta aatgaaattg caatagtttc aaatcagtat gatgtgactg
     8881 ttacctcatc actacctttg actgaggagc aaaaaaaccg tgttcgagca gttgttgcaa
     8941 agaaatttgc tgtaacagcg ggtcgtttaa ttgaaaaagt agatccttcg cttattggag
     9001 gatttattat cagcgtcaat aataaagtaa ttgataccag tattcgtcgt caattgcaag
     9061 catttaaaat gaatttaaaa tagaaagtgg tgtgactttt ggcaattaat gcacaagaaa
     9121 ttagcgcttt aattaaaaag caaattgaga acttccagcc aaatttcgat gtcacagaaa
     9181 ctgggatcgt tacctatatt ggtgacggta ttgcacgtgc ccgtggatta gataatgcca
     9241 tgagtggtga acttcttgaa tttgaaaatg gtgcctatgg gatggctcaa aaccttgagt
     9301 caaatgatgt gggtattatt attcttggtg atttttcagc aatccgtgaa ggagatgtgg
     9361 ttaagcgcac cggtaaaatc atggaagtac cagttggtga agctctcatt ggacgtgtgg
     9421 taaacccact tggacaacca gttgatggct tgggagatat tgaaacaact ggctttagac
     9481 cagtagaaac gccagctcct ggtgttatgc agcgtaaatc cgtttctgaa cccttacaaa
     9541 ctggattaaa agcaattgat gctttggttc caattggtcg tgggcaacgt gagttgatta
     9601 tcggagaccg tcaaacagga aaaacatccg tagctattga tgcgattttg aaccaaaaag
     9661 gtcaagacat gatttgtatc tatgtggcta ttggtcaaaa agaatcaacc gttcgtacac
     9721 aagttgaaac ccttcgccgt tacggtgcct tggattatac tattgttgtg acagcgtcag
     9781 cctcacaacc atcaccattg ctcttcatcg ctccttatgc gggtgttgcc atggctgaag
     9841 aatttatgta ccaaggcaag catgtattga ttgtctatga tgatttatca aaacaagcgg
     9901 tggcttatcg tgaattgtct ctcttgcttc gtcgtccacc aggtcgtgaa gcttacccag
     9961 gggatgtttt ctacttgcat agccgtcttt tggaacgctc tgcaaaagtt tctgatgact
    10021 taggtggagg ttctatcaca gctctaccat ttatcgaaac acaggccgga gatatctctg
    10081 catatattgc gactaacgtt atttcgatta cagacggaca aatcttcttg caagagaacc
    10141 tctttaattc aggaattcgt ccagcgattg atgcaggatc atctgtctca cgtgtaggtg
    10201 gttcagctca aattaaagcg atgaaaaagg ttgctggtac tcttcgtttg gatttggctt
    10261 cctatcgtga attagaagcc tttacccaat ttggttcgga ccttgatgct gcaacacaag
    10321 caaaacttaa tcgtggtcgc cgtacggttg aaatcttgaa gcaaccattg cataaaccgt
    10381 tacctgttga aaagcaagtc gttattcttt atgcccttac tcatggtttc ttagatgatg
    10441 ttcctgtaga tgatattttg gcttttgaag aagctcttta cgactacttt gacgttcatt
    10501 acaatgattt atttgaaact atccgtacca caaaagacct tcctgaagaa gctgcccttg
    10561 atgccgctat taaagcgttt aaagaacact caaactttaa gtcatagagg gaggatacta
    10621 gtatggcagg ctctctaagt gaaattaaag ctaaaattat ttcaactgag aaaacaagta
    10681 aaatcactag tgccatgcgt atggtttctt ctgcaaaact tgttaaatct gagcaagcag
    10741 ctcgtgattt tcaaatttac gcctcaaaaa ttcgccagat tactacagat ttattgaaat
    10801 cagaattaac cataggatct gataatccga tgttggtgtc tcgtcctgtc aaaaaaacag
    10861 gctatattgt catcacgtct gataaaggac ttgttggagg ttacaattca aaaatcttga
    10921 aatctgttat ggatatgatt acagagtacc atgctgatgg tgactatgaa atcatttcta
    10981 ttggtagtgt tggttcagac ttcttcaaag caagagggat gaatgtggct tttgaacttc
    11041 gtggacttgc ggatcaacca agttttgaac aagtgagaca aatcatttca caatccgttg
    11101 acatgtttgt caatgagatt tttgatgagt tatatgtctg ttacaatcac catgtgaata
    11161 gcttaaccag ccaagtccgt gttcaacaga tgttgccaat ttctgacttg gttgctgatg
    11221 aagcagcaga agaaggtgtt actgggtttg aattggaacc taatcgtcat gatattttag
    11281 atcaattgtt acctcaattc acagaaagtt taatttatgg ggctatcatt gatgctaaaa
    11341 cggctgaaca tgcagcaggt atgactgcta tgcagacagc gacagataac gctaagaatg
    11401 tcattaacga tttgaccatc caatataacc gagctcgtca agcagcaatt acacaagaaa
    11461 ttactgaaat tgttgcagga gcaaatgcac tagaataaaa aataacgctg actagcgatt
    11521 aggttattgt cagcattcaa ttttatcaat cgagataaaa tgacttatgg gacttttcta
    11581 ttgaaagact gctcttttca gtataaaaga agttagcaag actaactaag ccctgactat
    11641 cgaatataag gagaaatgaa tgagctcagg caaaattgct caggttgttg gtccagtagt
    11701 agacgtcatg tttgctagtg gggacaaact tccagagatt aataatgcat tgattgttta
    11761 taaagatagt gataaaaagc aaaaaatcgt ccttgaagtt gcactggaac ttggtgacgg
    11821 tatggtgcga acaatcgcta tggaatcaac tgatgggctt acacgtgggt tagaagttct
    11881 tgacactggt cgtgcgatta gcgtaccagt aggtaaagaa actttgggac gcgtctttaa
    11941 tgtacttgga gaaaccattg atttggaaga accatttgca gaagatgttg accgtcagcc
    12001 gatccataaa aaagcaccat cgtttgatga attatcaaca tcatcagaaa ttcttgaaac
    12061 tggtatcaag gtaattgacc ttcttgcccc ttaccttaaa ggtggtaaag ttggactttt
    12121 cggtggtgcc ggagttggta agactgtcct tatccaagaa ttaatccaca atatcgccca
    12181 agaacacgga ggtatttcag tatttaccgg tgttggtgag cgaacacgtg aaggaaatga
    12241 cctttactgg gaaatgaaag aatcaggcgt tattgaaaaa accgccatgg tttttggtca
    12301 gatgaatgag ccgcctgggg cacgtatgcg tgtagcactt actggtttaa ccattgctga
    12361 gtatttccgt gatgtagaag gccaagatgt tttgctcttt attgataata tcttccgttt
    12421 cactcaggca ggttcagaag tatcagccct cttaggccgt atgccttctg ctgttggtta
    12481 ccaaccaacc cttgctactg aaatggggca attgcaagaa cggattacat caactcaaaa
    12541 aggatctgtt acatctatcc aagcgattta tgtaccagca gatgattata ctgaccctgc
    12601 gccagcaaca gcatttgctc acttggattc aacaactaac cttgaacgta aattgactca
    12661 aatgggtatt tatccagccg ttgacccatt ggcttcaagt tcacgtgctt tatcacctga
    12721 gattgttggt gaagagcact atgctgtggc aacagaagtt caacgtgtgc ttcaacgcta
    12781 ccgtgaatta caagacatca tcgctatttt ggggatggat gaattgtcag atgaagaaaa
    12841 aacattggtt ggacgtgcgc gtcgtatcca gttcttcctc tcacaaaatt ttaacgttgc
    12901 tgaacaattt accggtcttc ctggttctta cgtgccagtt gctgagacag ttcgcggctt
    12961 taaagaaatt cttgaaggaa aatacgatca ccttccagaa gacgcctttc gttcagttgg
    13021 tccaatcgag gatgtgataa aaaaagccga aaaaatgggc ttttagtgag gtgatgccat
    13081 gacacaaatg actgttcaag tggtaacgcc agatggaatc aaatatgatc accatgctaa
    13141 atgtatttct gtaacaacac cagatgggga aatggggatt ttacctaacc acatcaattt
    13201 aattgcaccc ttgcaagttc atgaaatgaa aattcgccga ggtggtgaag atgaaaaagt
    13261 ggactggatt gctatcaatg gtggtattat tgaaatcaag gataatgtgg ttactgttgt
    13321 cgctgattct gcagaacgtg accgtgatat cgatgtcagc cgagcagagc gagcaaaact
    13381 aagagcagaa cgtgaaattg cacaggctga aacgacccac aacattgatg aagttcgtcg
    13441 tgccaaggtt gccttgcgcc gtgctttgaa tcgtatcaac gttagtaaaa aataagtggt
    13501 gttgataaaa gacaaggata agtaagaaag gtttttaacc taatattttg tttgctgagt
    13561 ctttttaaca tcagtttctg agagtttgac catactacct tctctaaaca cgttattatt
    13621 gttgagagga gttatatggg ggaactcttt tttgtgtcca cgttgttagt agaaactcag
    13681 taatgacgct taatatattt ttaggctatc aacttagtca gagaggatgt caacttaatt
    13741 gataagttgg acggatcaag atggtatgtt ataataatcc tatggaatta ttaatgaaga
    13801 tcattgcctt ttcgtgtcat ttatttttta ttggccttag ttaccaatta ttgattagtg
    13861 tgattgactg gacaaaattt agtcattatc atccagaaaa tcttggccgg ctacgacttt
    13921 ttgtcttttt attagctact gccctagggt atttggtgag ccattgtatg ctggaattgc
    13981 ttcaaattag ccagacgctc ttttttgagt tccgctaata ctggaataaa agaagtatgg
    14041 tataatttta ctacgactaa gagatagata agggagaaaa ttgtggataa aataatcatt
    14101 gaaggtggtc aaacacgctt agaaggagaa gttgttattg agggtgctaa aaacgcagtt
    14161 cttcctttac tggcagcatc tattttacct tcaaaaggaa aaacaattct tagaaacgtt
    14221 cctattctat ctgatgtttt taccatgaat aacgtggtac gtggtttaga tattcgtgtt
    14281 gattttaatg aagcagctaa cgaaatcaca gttgatgcct caggacacat tttagatgaa
    14341 gccccttacg agtacgtgag ccaaatgcgt gcctctattg ttgttttagg accaattttg
    14401 gcacgtaatg gccacgcaaa agtatcaatg cctggaggct gtacgattgg cagtaggcct
    14461 attaatttgc acttaaaagg attagaggct atgggggcaa cgattactca aaaaggtgga
    14521 gatattactg ctcaagcaga tcgactgcaa ggtgccatga tttatatgga cttcccatct
    14581 gtaggagcta cccaaaactt gatgatggct gccactttag ctgatggtgt gaccactatt
    14641 gaaaatgctg ctcgtgagcc tgaaattgtt gatttagctc aatttttgaa taaaatggga
    14701 gctagaattc gtggtgctgg aacagagacg ttaacgataa caggcgtcac gcatttacga
    14761 ggcgtagaac atgatgttgt tcaagatcgt atagaagccg gtacatttat ggttgcagcg
    14821 gcgatgactt caggcaatgt tttgattaga gatgctgttt gggaacataa tcgtccattg
    14881 atttcaaaat taatggaaat gggtgtttct gtaacagaag aagaatacgg tattcgcgtt
    14941 caagcaaaca cgccaaagct caaacctgta acggttaaaa ccttgcctca tccaggtttt
    15001 ccaacagaca tgcaagctca atttacagct ttaatggcag tggtcaatgg agagtctact
    15061 atggttgaaa ctgtctttga aaatcgattt caacatctgg aagaaatgcg acgcatgggc
    15121 ttacaatctg aaatccttag ggaaactgcc atgattcatg gtggaagaca actgcaaggt
    15181 gctcctgtga tgtcaacaga tttacgtgcc agtgctgccc ttatcttgac agggattgtt
    15241 gctcaaggag ttacaatagt taataatttg gttcatttag atcgtggcta ctatcaattt
    15301 catgaaaaat tagcaaagct tggagctact attagtcgta gcagtgaggt ctagcttatg
    15361 acaaatggtt taaaatatgt cttagaacaa atgttattac ttttcataat tgctgccttg
    15421 gcgtgtcttt ttttagccat tggacttatg attggttata gttttatggg agatggccag
    15481 agtccatggc atattttatc tatggataag tgggcagagt tagtgaataa atttactgga
    15541 aagtaggcct tgcctacttt ttatagcatc tgagaggaag tatgttaaca aggaaaaaac
    15601 gaaaaagggt gcaagagcaa aaggttccta aaagagctaa tgccttactt actaagtcga
    15661 cacgacagta tcgaaatcgc tatcaaacgg ataatgctta tcgtaccttc aagccagcag
    15721 gttggcaccg attgcaccac ttaaaaggaa gttatgatca cgcggttgat agagggcatt
    15781 tgctaggtta tgctttagtt ggtggactga aaggttttga tgcttctacc ggtaatcctg
    15841 ataatattgc cactcaacta agttgggcta atcaagcaaa taaaccctac tcgacaggtc
    15901 agaattacta tgaaggttta gtatgtcgtg ctttagataa ggggcatcgc gtccgctacc
    15961 gtgtcaccct actttatgat ggtgataatc tactagccag cggtagccat ttggaggcta
    16021 aatcctctga tgacagcttg acttttaacg tgtttgttcc aaatgtccaa gcaggattga
    16081 ccgttgacta tcggacggga caaatagcta tcaatctcta agcgaaaagt cactgattta
    16141 agattgtcac taagcacttt ttttgataga atagaactaa ttgaataagt gtctatgaga
    16201 ctagtactaa tagagaaatt aacagggagt gagggccgtg actggaagcc ttcttaaggg
    16261 aactattagg aattcactca gctaaagaca attaattaag gtctgataac atcagataaa
    16321 aacggatggt accgcgtgtc aacgctccgc ttatcaagtg gaggagtggc acgctttttt
    16381 catcgctaaa agaagggaaa actatggatt tacaagcaca attagaggaa ttaaagacaa
    16441 aaacattgga gaccttacag tctttgacgg gtaatcatac caaagaactg caagatttgc
    16501 gcgtggctgt tcttgggaaa aagggatctt tgacagaatt actaaagggg ctaaaagact
    16561 tgtctaatga cttgcgtcct gtggttggta aacaggtgaa cgaagttcgt gacttattga
    16621 caaaagcctt tgaagaacag gccaaaattg tagaagctgc taaaattcag gcccaacttg
    16681 atgctgaaag cattgatgtg actttaccgg gtcgtcaaat gactttgggg catcgtcatg
    16741 tgttgacaca aaccagtgaa gaaatcgaag atattttctt aggaatgggt tttcagattg
    16801 ttgatggctt tgaggtggaa aaagattatt acaactttga gcgcatgaat ttgccaaagg
    16861 atcaccctgc ccgtgacatg caagatacgt tttatatcac agaagaaatc ttgttacgaa
    16921 ctcacaccag tcctgtgcag gcccgtactc ttgatcaaca tgatttttca aaagggcctt
    16981 taaaaatggt ttctccaggt cgtgttttcc gtcgtgatac tgatgacgct acccactcac
    17041 atcaattcca tcaaattgaa ggtttggttg tgggcgaaaa tatttccatg ggagacctca
    17101 aagggaccct tgaaatgatc atcaagaaga tgttcggtga cgaacgtagc attcgtttgc
    17161 gcccgtcata tttcccgttc acagaaccat ctgttgaagt ggatgtttct tgctttaaat
    17221 gtggtgggaa aggctgtaat gtctgcaaga aaacaggatg gattgaaatt cttggtgctg
    17281 gtatggttca cccaagtgtc ttggaaatgt caggtgttga tgcaaaagag tattcaggct
    17341 ttgcctttgg tttaggtcaa gagcggattg ccatgttgcg ttatggcatt aatgacattc
    17401 gtggtttcta tcaaggcgac caacgtttct ctgaacaatt taactaaaac gagtcgtttg
    17461 aagacctctg tcagctattt tatctaagat tgagtgaaag ttgataggta atgataatag
    17521 gttaattgtc taaggagaaa gtccctctcg cttcaacgtc ataagggttt tgacttggaa
    17581 cttaaaatac gaaaggaaat gactcaaatc acggcttgag agctttctct agccgagtga
    17641 tttgggcact aaaattatgt tagtatctta taaatggtta aaagaattag tagatatcga
    17701 tgtgacaccg gcagcacttg ctgagaaaat gtcgacaacg ggtatcgagg tagaaggaat
    17761 tgaagtccct gcagatggtt tatcaaaact ggttgtgggg catgtgcttt cttgcgaaga
    17821 tgtgccagag acacatttgc atttgtgtca ggtagataca ggtgatgaaa cgccacgtca
    17881 aattgtgtgt ggcgcaccaa atgtgaaagc agggattaag gttatcgtgg ctgtgccagg
    17941 tgctcgtatt gcggataact ataaaatcaa aaaaggcaaa atccgcggca tggaatctct
    18001 tggcatgatt tgttccttgc aagaacttgg tttgtcagat agtattattc caaaagaatt
    18061 ttcagatggt attcaaatct tgccagagga ggctgtgcca ggtgatgcta tttttaaata
    18121 cctggacttg gacgatcata tcattgaatt gtccattaca cctaaccgtg ctgatgcgct
    18181 ttctatgcga ggtgtggcac atgaagtcgc agctatttat ggcaagtcag tatcattccc
    18241 ccaaaaaaat ctacaagaat ctgataaggc aacttcagaa gccattgaag tggccattgc
    18301 ctctgataac gttctaactt acgctagccg tgtcgttgaa aatgtcaaag ttaagccaag
    18361 tccacaatgg ttgcaaaacc ttttaatgaa tgctggtatt cgtcctatta ataatgtggt
    18421 tgatgtgacc aactatgtgc tcttgtattt tggtcaacct atgcatgctt ttgattatga
    18481 taaatttgaa gaccataaga ttgtggcgcg tgcggctcgt caaggggaaa gtttagtcac
    18541 acttgatggt gaaaaacggg atttgaccac tgaggatctt gttattaccg tggctgacaa
    18601 accagttgct ttggcaggtg ttatgggtgg tcaagccact gaaattgatg ccaactctca
    18661 gacggttgtt cttgaagttg ctgtttttga tggcaaatcg attcgtaaaa ccagtggtcg
    18721 tttaaacctt cgttcggaat cctcttcacg ttttgaaaaa ggggttaact atgcaactgt
    18781 tttagaagcg cttgactttg cagcggctat gttacaagaa ttagcagaag gtcaagtctt
    18841 atcaggccat gttcaagctg gacaattgcc aacagaacct gttgaagtct caactagcct
    18901 tgactatgtg aatgttcgtt taggaacaga attaactttt gctgatattc aaaggatttt
    18961 tgaccaactt ggatttggtc taacaggaga tgaaacaagc ttcacggtag ctgttcctcg
    19021 gcgtcgttgg gatgttagta ttccggctga tttggtggaa gaaattgcgc gtatttatgg
    19081 ttatgataag ttgccaacaa ccttaccaga ggtaggagga acggctgcag aattaacgcc
    19141 aacacaggct cttcgtcgta aggtccgtgg tttagcggaa ggattgggct tgactgaaat
    19201 catttcgtat gctttgacaa caccagagaa agctgttgag tttgcagtgg caccaagtca
    19261 cttgacagaa ctcatgtggc cgatgtctgt ggaacgttca gcgcttcgtc aaaatatggt
    19321 ttcagggatg ctagatacgg ttgcctataa tgtggctcgt aaacaaagta acttggctct
    19381 ttatgaaatt ggaaaaatct ttgaacaaga agctaatcca aaagaaaacc ttcctaatga
    19441 agtgaaccat tttgcttttg ctatttgtgg cttagtggca caaaaagatt tccaaaccca
    19501 agctcaggca gtggattttt accatgctaa aggtatcttg gatactttat ttgcaaacct
    19561 taacctcaag gttcaatatg tgccaaccaa agacctagca agcatgcacc caggacggac
    19621 agcgcttatc ctattagatg agcaagtgat tggatttgtt ggtcaagttc atccaggaac
    19681 agcaaaggct tatagtattc cagaaaccta tgtggcagaa ttgaatatgg cggctttaga
    19741 agcagcgttg tcaagtgatc aaacttttgt ggagattacc aaattccctg ctatgacacg
    19801 tgatgttgcc ttgttacttg accgtgaagt gagtcatcag gccattgtaa cagctattga
    19861 atcagcaggt gtaaaacgct tgactaagat taagctcttt gacgtgtatg aaggagtaac
    19921 tattcaggct ggcaaaaaat caatggctta tagccttact ttccaaaatc caaatgacaa
    19981 tttaacggat gaagaagttg ccaaatacat ggagaaaatc accaaatcat tgacagaaca
    20041 agtcggtgct gaagtacgct aagaaaaaaa actgggcctc taaggctccg tttttttctg
    20101 ttataataga ggaaaaaaga actccagttc aaatgattgg agaaaaacat cagaaggtgg
    20161 aggaaaatca gaatgtatca aacaaaaata acaggcaacc gcctttaccg cacggtttcg
    20221 aaaggctacg gagatagtgt aaccttattt ggaaaaactg atcaagggga aacccctatg
    20281 agtttactgg ttattgcctt gtcttcttgt gtgactatgt gtgtgcaggg tttttaccat
    20341 cgtcaatttc agcaagacac acttgctgtt tctgttgagg ctagttatga ggaagaagtt
    20401 tttgaactac ttgttaggat tgatgatgtt ttagatgaca aaaagaaagc tgatttattg
    20461 gcttacattg gaacttattg tcgtgtgaaa caactcctca aacctgaagt tacggtgacc
    20521 attacgttgg aaggactgtc tcatgtctga tgctagtttg aaaacttacc aaaaacaatg
    20581 ggcttatcaa aagtattggg tcatggcaca ctcacagcag cattacaatg ctttaagaga
    20641 attgtttaaa ggaaatcagt ggtcagaaga aaaggtgttg acttttcatt gtttgattga
    20701 agaagcgcaa gccattcctc ccacagtaaa aagcttacga acggcatatc agcatgtttg
    20761 ggggtatttt aaaaaggtag cgagtcagga agaaaaagac catttcaaag atttggatgc
    20821 tcagttggaa actaaatctg aggagatgct atgcttttta caagagatga ctgctcatta
    20881 tcagccctct tatctgctat cttgccgttt gattaccaaa ggtccttaaa tgctaaacag
    20941 aaaagtaggg atttctggga agttatgcta agctagtgat ataaataagg gggaatgaga
    21001 gatgttttta gcgcttaatg aaatgaaaca atccaaattg cggtatggtt tgattgctgg
    21061 tttgctatgt ttggttgcct atttgatgtt ctttttgtca ggattggcct ttggtttgat
    21121 gcaggagaac cgctcggcgg ttgatctttg gaaagctgac agtgttttac tagccaagga
    21181 tgctgacgct accttgacct tatcacaggt gtccagagct caagaaaatc aaataacagc
    21241 agacaaggta gctcctcttg ctcaactcaa taccgtggcg tggtcagtta aaaatcctaa
    21301 ggatgccgac aaagttaagg ttagcctttt cgggattgat tctaatagct ttattcgtcc
    21361 taacattgta aaaggtcgat tatttaagac taacaaagag gttgttttgg atcaaagcct
    21421 tgcaaaagag gaagcttttg cgattggcaa ggacttttac acatcgagtt ctagtcaagc
    21481 attaactatc gttggttata ctcaaaatgc tagatttagt gttgcaccag tggtttatat
    21541 gaatttggaa gcttttgaaa cattaaaata tggagaacca ctaccaaaag ataagcaagt
    21601 tgttaatgct tttatcacta aaggaagttt aacagattat cctaaaaaag acttccaaaa
    21661 attagatatt aaaaccttta ttactaaatt acctggttat agcgctcaac ttttaacttt
    21721 tggctttatg attagttttc ttgtcattat ttcagctatt attattggta tttttatgta
    21781 tattttgact attcaaaagg cacctatttt tgggattatg aaagcgcaag gtatcgggaa
    21841 tataacgatt gcacaagcag ttctgctgca aaccttcttg ttaagtgctt taggaagcgg
    21901 tttagggtta ttgggaactt ggttgacgtc tttagtgctc cctgaagcag ttcccttcca
    21961 aagtaattgg ctagtttact tagcgatatt agtcagtatg gtttgttttg ctttgttagg
    22021 aaccttattt tctgttttta gcattgtacg aattgatcct ttgaaagcaa ttggatagga
    22081 ggtagtgatg tcggttttaa catttaaaca agtgaccaaa acatttcaag atggtcatca
    22141 cgaaatcaac gcattaaagg caactgactt ttccattgaa gctggagaat ttgtggctat
    22201 tattggacca tcaggttctg gaaaatcaac ctttttgacc attgctggtg gcttacagac
    22261 cccaagctct ggtcagctaa tcattgatgg cactgattat acccatttat cagaaaaaga
    22321 gcgctcacgc ttgcgcttca aatccgttgg ttttatctta caagcttcca atctgatacc
    22381 ttttttaacg gtccagcagc agttggaatt ggtcgatcat ttgacaggaa gcaaagaaaa
    22441 agccaaagcc aatcagttat ttgacgattt aggcattaca gggctaaaac atcagttacc
    22501 tcaagaattg tccggcggag aaaggcaaag agcggctatt gcccgtgctc tttaccatga
    22561 tccggcactg atattggcag acgaacctac tgcaagtttg gatactgaaa aagcctatga
    22621 agtggtcaaa ctgttggcta aggaaagtaa agaaaaaaac aaggccatta ttatggtcac
    22681 tcatgatgat cggatgctaa aatattgtga caaagtctat cagatgcaag atggagagtt
    22741 acgtcaagag agatagtaga ctggtaagag tttatgatat tagaagaaaa aaccaaacaa
    22801 gcacagcact tgtctggttt ttttctaagt gtgactatta tcaatacgtc accatttccc
    22861 taaagcttac tatacgggat gctaaaggta tcaccatgct caaagtcgcc atattggtaa
    22921 cctcggtcaa accaacgttg gcgttgctta gaagttccgt gggtaaaact atcaggaacg
    22981 gctctgccat aggtttcttc ttgcagagta tcatccccaa ctgcgtgagc tgctgccatt
    23041 gcttcctcaa tatcaccttt ttccaaaagg ccttgccctt gaacataatt agcccaggca
    23101 ccagcatagt aaccagcttg taattccaac ttgacgttga gctgattggc tttagccttt
    23161 gattttcctt gtctagcgct agcgtaatta tccataattc ccaattcatt ttggatgtgg
    23221 tggccaactt catgagcaat cacataggcc atagcgaaat caccttttgc gccgtacttg
    23281 gttgatagtt cattataaaa agaaatatct aaatacactt tttgatcgcc tgggcaatag
    23341 aaaggaccag aagaggcttg ccccctgcca caagcggttt gagttgctcc agtatataga
    23401 actaatgttg gtttatgata agttaaccct ttctcacgaa atgtcttagt ccaatagtcc
    23461 tcggtagagg cgaagacctt actgacaaaa gaaacctgtt cttgactagc cttgtcacca
    23521 tttgtacgcg ttacttggct tgattgatag gcattgttgt tggtagaaga aggcttgcct
    23581 cctaaaacac cagataggcc acctcctccc ataactagga gaaggagaag aataaccagc
    23641 ttggttttcc aacctcctcg tgaaaagagt aattgaagca ggagaccgcc ccctaggccg
    23701 ccgcccccaa aagaaccaga cgattgacca cgtctatctt caacctgttg actttctctc
    23761 aaatcatctg
//
LOCUS       DATURV010000023        22354 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DATURV010000023 DATURV010000000
VERSION     DATURV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22354
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.023"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..2003)
                     /gene="recG"
                     /locus_tag="VCF75_000883"
     CDS             complement(<1..2003)
                     /gene="recG"
                     /locus_tag="VCF75_000883"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEP2546460.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPDTLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAALKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSVIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     HKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAKNIRKK"
     gene            complement(2378..3832)
                     /locus_tag="VCF75_000884"
     CDS             complement(2378..3832)
                     /locus_tag="VCF75_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546461.1"
                     /translation="MERLGGADLDYCYHNHHDTSMISAEQLVFTYHDQKNPACQVPTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQNLLALTDEQQQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            complement(3769..4449)
                     /locus_tag="VCF75_000885"
     CDS             complement(3769..4449)
                     /locus_tag="VCF75_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP2546462.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(4446..5039)
                     /locus_tag="VCF75_000886"
     CDS             complement(4446..5039)
                     /locus_tag="VCF75_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEP2546463.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPILLMWLLKAAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSVFAHYLLKKHFIKSGLLS"
     gene            complement(5036..6706)
                     /locus_tag="VCF75_000887"
     CDS             complement(5036..6706)
                     /locus_tag="VCF75_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546464.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGIYHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LYEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGFLKEVNKK"
     gene            complement(6699..8462)
                     /locus_tag="VCF75_000888"
     CDS             complement(6699..8462)
                     /locus_tag="VCF75_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEP2546465.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQATERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQETINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLNTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(8459..9295)
                     /locus_tag="VCF75_000889"
     CDS             complement(8459..9295)
                     /locus_tag="VCF75_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546466.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQIWLEQEELATISIKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGQLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNGIRTQRRDRNK
                     NR"
     gene            complement(9292..10314)
                     /locus_tag="VCF75_000890"
     CDS             complement(9292..10314)
                     /locus_tag="VCF75_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP2546467.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYEAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYALAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(10316..11200)
                     /gene="isdE"
                     /locus_tag="VCF75_000891"
     CDS             complement(10316..11200)
                     /gene="isdE"
                     /locus_tag="VCF75_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEP2546468.1"
                     /translation="MIKRCKGIGLVLMAFFLVACVNQHPKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSVNPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHP"
     gene            complement(11184..12059)
                     /locus_tag="VCF75_000892"
     CDS             complement(11184..12059)
                     /locus_tag="VCF75_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEP2546469.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDSQKPVDTKLGESQDEPHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            complement(12256..16083)
                     /gene="shr"
                     /locus_tag="VCF75_000893"
     CDS             complement(12256..16083)
                     /gene="shr"
                     /locus_tag="VCF75_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEP2546470.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKPVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDITITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLEKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVENGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVPYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKVTGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            complement(16574..18103)
                     /locus_tag="VCF75_000894"
     CDS             complement(16574..18103)
                     /locus_tag="VCF75_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP2546471.1"
                     /translation="MDKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSDNKA
                     EAENQVDDKSTDHSKSTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSKQLPEARTPI
                     QSLSPYVSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKS
                     TGIAYDSTRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAF
                     DLDPTQASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFT
                     DTTGSGKRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLG
                     YQASSYAWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKVGYALSKTPKVGYAISFAP
                     GQAGADGTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSK
                     N"
     gene            complement(18190..19290)
                     /locus_tag="VCF75_000895"
     CDS             complement(18190..19290)
                     /locus_tag="VCF75_000895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEP2546472.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKEVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            complement(19287..19643)
                     /locus_tag="VCF75_000896"
     CDS             complement(19287..19643)
                     /locus_tag="VCF75_000896"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEP2546473.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWSGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            complement(19759..22278)
                     /gene="secA"
                     /locus_tag="VCF75_000897"
     CDS             complement(19759..22278)
                     /gene="secA"
                     /locus_tag="VCF75_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEP2546474.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVNEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAVDAIVTFARTSLVPEESISAKELRGLKDE
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
BASE COUNT         6872 a         4637 c         3839 g         7006 t
ORIGIN      
        1 tctttttttc taatattttt tgctactaac tgccactgtt tttcatatat ccagttagga
       61 tcagaaacaa tcgctgcaga aactttgcgt gcttcttcta aaataggata atcctcaacg
      121 atatcagcta cttgaaattc tggaattcca gactgacgag taccaaagat ttcaccagaa
      181 ccacgcattt ttaaatccga ctcagccaaa acgaaaccat ctgtcgtttc tgtcatgatt
      241 gtcattcgtt ttttccccga atccgttttg ggattagcca ctaaaacagc gtatgattgt
      301 ttatgtccac gaccaacacg cccacgaagt tgatgtaact gacttagtcc aaaacgatcg
      361 gcatccataa taatcatgat cgttgcattt gggacattta cccctacttc aataactgtt
      421 gtggatacta aaatatgact ttttttatcc ttgaaatctt gcattatggc atctttttca
      481 tcatttttca tacgtccatg tacaagagca accttagcaa ttccttcaaa ataagtagat
      541 aattcagcat gcaatgctac tgcattcttt aaatctaaag cttctgattc ttcaatcaac
      601 ggtgaaatga catacacttg agcatctttt tgcaattcac cttttaccca ttccaacaca
      661 gtccctagct gctcgtgttt cacccagcgt gtcataatag gtttacgacc ggcaggtaat
      721 tcatcaataa cagaaacatc catttctcca aaagctgtga ttgctagagt tcggggaatt
      781 ggggtggctg tcatcattaa aacatcagga ttttctccct tttcacggaa aattctacgc
      841 tgtttaacac caaaacgatg ttgctcgtct gtaatgacaa gccccagttt atggtactgt
      901 accgagtctt ggataagagc atgagttcct acaatcatat caaccgagcc atttgcaata
      961 gctgctaaaa ccgtacgctt gagagcggct ttcatacccg aagttaatat agcaattgat
     1021 agatctggaa ataactcttg cagactaatg tagtgttgtt cagctaggat ttccgttgga
     1081 accatcaaag ccgattgaaa acctgctgta taagctgcat acattgatag accagcaatg
     1141 actgtctttc ctgatcctac atctccttgc aataaacgat tcatatgagc tcccgatgac
     1201 atatcagata atatgtcatc taaagagcgc ttttgagcat tcgttagaat aaaaggtaaa
     1261 gaggaaatct ttgtctccat agcatgttta ctataaagaa taggcaaacc atttgtttca
     1321 gatttatttt cggctttcaa aacttgaagg ttcatttgaa agtaaaataa ttcttcaaat
     1381 ttaatccgac ggagcgcttg cttatactct gtgatatctt ttgggaaatg catagctaaa
     1441 caagcctgac tacgacccat caatcggtat ttttccaata aagtatctgg taaattttcc
     1501 ttcaattcca aatgcgcatc gatttcaaaa gctgacttaa tagctttaat caaagtagac
     1561 tgtgaaattc cctgagctac gcgataaaca ggttgcatgt catcttcaac ttgagctaaa
     1621 actttcatcc cagtaatagc tgatttagtg gcatcccatt taccaaaaac agctacctct
     1681 tgaccaagtt ctattttatc agctaaatag ggttgattaa aaaaactaac atttaagaca
     1741 gcttcccctt gacgcaattt gaaacttaaa cggttacgtt taaaaccata atattgtaca
     1801 ttagctggag taacgactaa gcctgtaatg actgcttttt caccatccac taaatcaaaa
     1861 acagattttg atttaaaatc ttcatagcga aacggataat aaagcagtaa atcttctact
     1921 gtataaatat ctaatttctg aaatttttct gctgattttg gtccaaatcc ctttaagttg
     1981 gacataggag ctgttaatat cattatcttt ttcccttcac aaaaactctt gtcagattga
     2041 agacaaagtt tgtcgtgata ctttccttag gtggtgctta cggcagcgac gttctttgga
     2101 attccatgcc tttatttttg agcctgaggt ctcaaaaatc ccgagtggcg tcaatctcgt
     2161 attgacgcca ctcttttacc acagtaaaaa gcatctgttt tggctcactt tgtgtgtaaa
     2221 aatcggaaac cgaatgtcaa atcagcagag ctattcatgc tttttgagca taaaactggg
     2281 gttgtctacg ttctgactct tgtatctatt atacaagagt tcctatttat tttcagataa
     2341 aagcctttta gtacttttaa acagttctag gggctcgtta aggtagcatt tgataaatgg
     2401 tatcgcagac actacttaga agttcttcat catgtgaaat gaggatgaca accttatgtt
     2461 gggtcttcag ctgcatgaga agattagcta aagccttcat ttgtaacaaa tcaagtccgc
     2521 tacttggctc atcaaaaatt aagatatctt tatcagccaa aagactagcg gcaatcatca
     2581 ctctttgttg ctcacctcct gacaaactag ctggatgacg ttctaaaaga gttgttaaag
     2641 ataaccgctc tattacttct gtcgtccgtg gatgtcgttc atggcccaag ttgacttcct
     2701 ttgaaaccga ttctgcaaat agttgcaaag aaacttcttg taagacaata ctagtctttg
     2761 ataaacgttg cttggcagat aaaggtatgc cttgaaactt aatagttgct tttttatcgt
     2821 ctaagatacc agctatataa taaactaatt gcgattttcc aagcccgttt gatcctgtaa
     2881 tgccagaaat acttcctgca gcaaaactaa gctgttcaat acatctaagt attttgcttc
     2941 ccgctctaac tgttagatgc tcaatacaca ggctatcatc tgggcgataa tgttgactct
     3001 caatcttacc agctaacaca ggttttaggt cagacaagtc taagcgacgt agtcccatat
     3061 cttgctgttg ttcatctgtc aaggctagca aattttgtgt tgttaactta tctgttaatc
     3121 taccattttt aaagtagaaa aagttatctg ccaaatccat caagtaatgt aagcgatgct
     3181 cggctacaat aatggttttt cctgctgctt ttaattgggt aagataggct ttaacagcag
     3241 ctatgcctgc actatctaag ttagcagttg gctcatcaaa aagcataata ttagtacctt
     3301 gcataatggc cgttgcaata gcaactcttt gtttttgccc tcctgacaat ccaaacatat
     3361 ccttgttaag gagctctgca aaagcaaaat cttctgctaa agtccaaaga cgcttcatga
     3421 taacctttgc atctagacct tgattttcac agggaaagac cagttcgtgt tggacttcgc
     3481 gatagaaaaa ctgactggct ggattttgaa aaacagaggc cacagatcta gaaaatgttt
     3541 caacactgtc tctgcctgct tggcaatcag ccacatctag tctaccttca tattttccag
     3601 cataataatc aggaattatg ccattaagaa gttttaaaaa ggtagattta cctgaaccag
     3661 aaggtccaca caaaacaatg aattgtccag aggcaatttg acaagtcggt acctgacaag
     3721 ctggattttt ttgatcatga taggtaaaaa caagttgttc tgctgaaatc attgaagtat
     3781 cgtgatgatt atgataacag taatccaaat ctgcaccccc caatctttcc atgttaagtg
     3841 tgagctaaag cattcactgg tgcctttgtt gactgctagc cctttagtta gactagcgat
     3901 agttagttct tggctacttc ttatcaaaga taaaagcaat ggaaccatta aatactccaa
     3961 gtattgttta ggtctgatca aaatagacca ttttgttaaa ataatccccc tgattgttaa
     4021 agagcgatga ataacacaac attcttgtct aatcattggt atgaaacggc acataacggc
     4081 taaggtcaag agccagactt ctgggaaatg ccatttgcgt aatccatgaa ccaattcgta
     4141 gatggtagtt gtcttaatag taattaaacc tgctagtaaa gaagggaaaa ctaaccttcc
     4201 tgctattgag acaaaactca ataagtggtc aagataagaa gggaaaatcg atagcgggat
     4261 tacagataga ccgatcatta acagataaac aaccgccaac ttgaacgcta gctttttctt
     4321 atttaaagca gacaaaagta ataagagaaa aataacaatt ataaattcta aaaagccatc
     4381 aacacgaaaa aagaagcagg cattggcaag taccaacaaa agcagtttgg tgcgaacatc
     4441 aagcctcatg ataacaatcc tgatttgata aaatgtttct ttagtaaata atgtgcaaaa
     4501 actgacccta ataaggctcc tgcaagaaca attcctacaa accaaataac atgccctaaa
     4561 ctaaaatcat acataatgcg attgacatag gtcatacttt taccgcgggc taacaaactt
     4621 tcaacatagg cagctttcaa caaccacatc agcaaaatag ggccactatt gacaaaagca
     4681 aaaaccatat aagataggtc attaaaaaac cttgacttgt attttcctaa agaagcaata
     4741 atatccgcta ttaaaccgaa aataatgctg ggaaacatcg cggcaaaaaa gtgaccacta
     4801 aggaagaaaa aacctcccat catcaatcct aataatgtta ttggcccaaa tgtttgcaat
     4861 ttagcaatca aaagaaaata aattgttccc cccagtaaag ctgcaaaggc aggggcatat
     4921 aacatatttc ctgagcgatc aaaaaagatg ttaataaacg tgcctagacc aacacaaata
     4981 aagtagagcg cggcaaatgc gcctgttatc ataatatctt ttattgttaa ctgtttcatt
     5041 ttttattcac ctcttttagg aaaccatttt ttacgcaaaa tagatgatca gcacaagcta
     5101 gggttgatgg cctgtgagaa attaagaaga ccaatccttg acaatgtgtt ttaattaaat
     5161 caagcaagat agcttcatat agagaatcta aatttgacgt gggttcatca aaaatatagc
     5221 aactcgcatc tttcaataaa gctcgcatca actctaaacg ttgaccttct ccagctgaaa
     5281 aatcagaagg actcttgata atcgtatcca aaccttcctt acaagctaac agtctgtctt
     5341 tcataccaca agcctcagct aaattccaaa tggactcatc tgaaatatct tgacggccaa
     5401 aggtaagatt ttcacggata gactgttgga aaagctgcgc tgtttgtggc acataagcaa
     5461 tagtatcttg taatttagca gcatttagta agcaactatt cctatcatta agaaaaatat
     5521 ctcctgtctt ccaattatac catttcataa ttaatttcac caaagttgat ttaccagatc
     5581 ctgattcgcc tttaatgcca ataatcccct tttcttgaaa ggtaactgat aaatccttaa
     5641 aaaccagctc ttggcgcttt ggataagcaa aactgaggtc ctcaaaagaa acactcctta
     5701 aacgatctat ctgttttgtg ccttcgtcta ctatgacatt ttcgtctaga aggtcaaaaa
     5761 tatttcgagc cgcattcata gcacgcttaa aacctaatgg caaacgtcct agttctaaaa
     5821 aaggagaaaa cgaagcagtg aaagcaataa aagttaataa gcctttatca aatgataagc
     5881 tgtcatggta tatcccatca aaaaccatat aagaaaatgc taagatcatt aagccaagcc
     5941 agaaaaaagt caatgccatt tgcaaaaatt gggcttgcgc tacgttacga tcaagagcat
     6001 ttacatgatt actttttttt attaatcgtt caaattgctc gtctaatacc tgaaattgca
     6061 aaagatcttt gactgatcgt aaactctcta aaaaataaga caggtaatct tttcgacctt
     6121 cattttggct ttttagtaaa acctgtaaaa tattagcaaa ataaatgggt ataacaatgg
     6181 ctaaacaagc ataggttgcc aaagctaata aggctaactg ccaactactt tgacaaaagt
     6241 accagaacat taaacctgca gacaatattg ctgtacaaat aggtgcgatt gtgtgggcaa
     6301 aaaaaacttc taatgcctca atatcttctc caatcatttt aagcaaataa ccactgtctt
     6361 gactatccaa gcaagctgga gaaagagctc ttagtttctt aaagatcaag ttcctaaaag
     6421 ctgctaaact atgaaaagca acaaaatgac caaaataatg ctctccataa cgaacaaaac
     6481 ctcttaaaaa ggctagagca atcaaaagat ataaagctaa tattggaatc acttcttgtc
     6541 taatagctag tagtcctaaa taagctattc cagttggaat acttaccgtg atgacaaaac
     6601 ccataacagc aaagccaaca gccaagataa tccaaggtag aagagttttc attagggtta
     6661 ataaacgcca aaccaggctt aaggtagatc tgttaggttt agccataaat acttgcctcc
     6721 agtcttgctt gagtatcaac taggtgacga taagttggtt ggtcccgata aaggtcgcaa
     6781 ggtttgccta aacaagcagg ctgccctgtg tttaaaaaga gtacttggtt agctcctttc
     6841 agtcgagaca tcttatgagt aatcacaata actattgctg tcttagctaa tctcgttatt
     6901 aaattgtcaa taatacgttc attttctgca tctagactag atgttgcctc atcaaagata
     6961 taaagagacc tcttacttaa tagagctctt gcgcaaatca cctgttgctt ttggcctggt
     7021 gataacaggt taccattttc accaactatt gtgtcaagac catctggtag ccagttgatg
     7081 aagcttaaca aaccatgttg atctatccaa tctaagattt cttttttaga gagatttgct
     7141 gctaacctta aattatcgta aatgctacgg ttcaataagg tagattggtc cgatacataa
     7201 agaacctgct ggttgatagt ttcttgcgac aaattgtcac tgtctaaacc gtcaaataaa
     7261 atatgcccag tcgttgcaga ctggcgtttc aagaggagtt gagctagact tgtttttcct
     7321 tgtcctgaca cccccgcgat agctgttagt tggcccttcg ttaatgtcat ggtaacccca
     7381 ctaagcactg tttttttctc ataagctaaa gaaatatttt ggatgtcaat ctggttaaag
     7441 tttattgctg ttttactctt gttatctttc cttgctggaa cactatccaa gaaactaaag
     7501 atacggtcag ccattttggt attcatcatg accaagtgca tgccataacc ctgctcacga
     7561 atcggtgtaa aaaattctgt agcaatcaag agaaaaaaga ggaaattaaa aaaagataac
     7621 tggcctgttg ccaaagcttg aacggctaaa aaacctgaca aaccaattcc cagatacata
     7681 actgcatcca tataaccaac tgcttgtaac tgaaagccaa gcaaagacat ggtagccttt
     7741 cgaaactgtt ctgccttacc cgaaaattct tgctcgtaac gttctgttgc ttgataagaa
     7801 tacagtgtat taagaccttt taagtcatcc ataaataaat taccaacatc catataagaa
     7861 gaccaataat gactcataat ttgcttagac cgcttttgca tagcaacaat tgaaagtgga
     7921 atcagaggca gaactcctaa aaagacaagg cctgccaatg gataaattaa aaagactagt
     7981 atcataatag ttgtgcagtt ccacttggtt ctcatagaca aagacaaata gtaaccataa
     8041 taagtatcca aactatcaat cccctgtgaa gccaacgtta aaatatcagc atcactggca
     8101 tgagcatcaa attgcccgtc taaatcaata aaagcctcaa agaaagattg tttgagtgaa
     8161 tctctagcaa attgtgaagc gatcccttgc agttgcttgc ctaatatagc aagaacaaag
     8221 cctaccacat ttaaaagtag gagtaaacct gccaagcgtc ctaaggcaat agcatgaccc
     8281 aaaataaagg tcgaaaacgt tttggcaatg aggtaaaaac tgatcatcct catcacaaat
     8341 tgtaaccaag ataaaaaggc tgacacataa agcaaatacc tttttggtgc aattcgttca
     8401 cgtaatcgtt ttaacagtct tttccttttt ccccttgata ttgtatcttc tgtttccatc
     8461 atctgttctt gttcctatct ctcctttgag ttctaatacc attggtttca ttgaccaatg
     8521 aagtgaggac aaactgttga ccatttctct caacaaaggg caattcaaca ccaaagacat
     8581 ctctgataaa ctgttgatca aggaggtctc ttggttgccc ttgggcataa agttgcccat
     8641 ctttcagtcc aataagatgg tctgaaagag acaaggcctg attgatgtca tgtaatacta
     8701 acacaatcgt taggcctagc tcttggttga ttttagtaat cagcttgaga atgtcaagct
     8761 ggtacttaat atccaaataa gtcgttggct catctaaaaa aataatatct gtatcctggg
     8821 caagggccat ggcaatccaa accctctgct gttcaccacc agaaaggtgt tggatgtcac
     8881 gataaacata atcactcaaa gccgttactt ctaaggccca agcaaggcgt tcctcatctt
     8941 ctttggtagt agttttaaaa aaagaacgac gggcaagcct agccatggct accaattcac
     9001 ccactaacaa accttcgacc acttgatggt attggtgtac cactgccact tttttcgcaa
     9061 aagccttgat agaaatcgtt gctaattctt cctgctcaag ccaaatctgc ccttgtttta
     9121 acggaaggtt tttggttaat aattgtaata aggttgactt gccactgcca tttgccccca
     9181 taatagtggt aattttccct tttggaatgc taaaggtcag gtcatctaaa acaagttttt
     9241 cctcataagc aaaggaaagg ttttgcacct gaatatgact actgttctta ctcatataat
     9301 atcagacctc cttagcaggt aaataaagta aggcccgcct acaatactca ttataatcgc
     9361 agggctaatt tccaatgggt aagcaagact tcttcctaaa gtatcggcta gcaaaaagac
     9421 aaaggctcca agcaaagctg aaaaaggaat taagatttgg tgcttacttc caaccagcaa
     9481 gcggctcata tgagggacaa tcaagcctag gaaagaaacc actccaacga tcgaagtggc
     9541 taccgaagcc aataaaacag caaccaagga aattcccaac cgtaacgctg tagcatcgat
     9601 tcctaaatga cgaatcacct gatcatctaa aagaagaagg ttacaagttt ttgacaataa
     9661 caaagctagt aacaagccaa tgaatgtata agggaataaa acccctacat cagcccatgt
     9721 tttttgacta atatgacctg ctaaaagagc attgaccatg ggactagctg aggtgaaaaa
     9781 gctggttaaa gcagttgata accccataaa taaagcatta atagcaatcc ctgttagaat
     9841 aagccgaata ggattaagac ccttcttcca tgctaaagca taaatcagca ggaaagacac
     9901 tccgcctcct aaaaaggata cgattggacc atacagcagc aactgcggta gaagtaaact
     9961 agagactaaa accataaaac tagcaccact acaaatccct ataatagcag ggtctgaaat
    10021 aggatttttc aacactgctt gaaaaagaac tcccgaaaca gctatccctg ctcctgctaa
    10081 taaagcaatg acaattcttg gaaagcgcaa atcataaatc aaagccactt gggggtcata
    10141 agccacaaac agtcccttga caattgcctc ataagaaacc gccaaaccac caagggataa
    10201 ggcaataacc gccaaaaata ggaggctgag aattaaaaga actaaataaa gaccacttct
    10261 gaaacgtttt ttagccaata gggattgatc cctttgtaaa tctgatgctg tcatgttacg
    10321 gatgatctcc cacgtggtca aaaagctgtg ttaaggtgtc caaggcttct gggtagttca
    10381 atttagcact catgccaaac agggtattgt ccaaatcata gactttccct tccttgactg
    10441 ccgtaaaatg tttccaaata tcattttcag caaattcttt gtcaaacatc acttttacct
    10501 tgtctggaat ggcgtgagct gttcgtaaaa tcaagtcagg ctccttagct agcatgtctt
    10561 caggattaac tgatagaaat tctttctcat ctgactgata aacattctca ccacctgcca
    10621 agtccaaaag attccctaca taagattggt tcgtcgccac caaataacta cctggcaagc
    10681 ccataagaat aagcacttta ggctttttct tccccttacg tttagcttgg aaagcacgat
    10741 aatagtcctg gtattgctgg cgcaattctt ttgcttcttg ttgacgttgg aaaaggttcc
    10801 ctaaatcatc aatagactgg tacatgccct caacacttcg taagttcaaa aaaccatact
    10861 cagtatccaa tttttgatac ttgggttcca aatcttcttg taaagaattg ggactcaaaa
    10921 tccaagttgg tttcaaagaa gcaatcaact ctatatcagg attcatgggt aaacccacac
    10981 gcttaacagc atcatagcgt ttaggaaggg tatataattt actatcacaa accccaacga
    11041 ggtctaaatt taaacggtca cagatatcaa ccacagcaac cgaagtggct acaattctct
    11101 gctgttcagt ctctttagcc gttttagggt gctgattcac acaagctacc aaaaagaagg
    11161 ccattaagac tagaccaatt cctttacatc gttttatcat tttttttcct ctttttccaa
    11221 acccatacag caaaccctcc taacatcaga aaaaaagttg gaaagtagta gatcatgatt
    11281 gataagtctt ttttagaccg aaacttatct tcttttttta actctaaaga agtcgttaag
    11341 cccatcttag aaggaagaat ttttttatct gatagactct tgttatttga tgagttggct
    11401 ttaggcttat tctgagttat catagcacct gtgtgaggct catcttgact ttctcctagt
    11461 tttgtatcaa caggcttttg actgtctttt acttcttgat tttgagaatt gtctgtttca
    11521 gtaacaagct gggccaacat attacctgaa tacttttgta ttaactctga agctgacagg
    11581 taaaagacca cttctctgcc cataggttca acaaacatgc ttcctcgaat gatactattt
    11641 acagtaggca aactaatagc aatatctagt gttgtcccat tggtatcagt acctttttgg
    11701 gtgatattgt aatcaactgc ctgaaagctt ccagttcctc caggttgtat ccagaactga
    11761 taattacccg aataatcagc taagctcata cgaaaagtaa gaacgatctt tccagcatca
    11821 gatacttcta gcatggcatc actatagaca gtcccttcaa ccataccttg accaatatca
    11881 aaggaatgct cccctccact atcttctatc tgcccagata tcgggtgacg ataatttctt
    11941 tgaataatac atccataaat ttgaccttta tctgcataca caagatttgt cattgttgat
    12001 aaacttatca tgaacactac tattacctgc aacaattgct ttattactac ctttgtcata
    12061 tgctcctctt gtctatcttt tcttaaacat cttatttcat tttttaaatg attcccattt
    12121 tataacatct ccaaatgtga aaaacacacc tatcttgcga ccacttcaac ctgtatcagg
    12181 aatcctgtcg gaaataggta tgtttttagc tcttgctgac tagctttttt gttatttcac
    12241 ttggtactaa gtttcttatt taaataatgt ctttcgacca ataatgaatc ccagagctac
    12301 gatcataaag ctagatagac ctgcaattaa gtgataacga gagctacttt cttcaccatt
    12361 tgattgttga ttggaacgat gtttcttatc agatttttta gtcttagtat cttttgccgt
    12421 tttaccttca attgatgttt gatggtcacg cactaactgc ttcaatttct ctgccttatc
    12481 agtatcatct gtcttagaag tttcttgagt tgattggccg gttacttttt tagccaggag
    12541 atcagttaag ttagcaatac cagtagctgc tgaaggagga cttgtaggtg ttttttcttg
    12601 ttgacgattt tgaggtcctt tcaactgatc tgttgaagtt agatggctat tttcttgtgg
    12661 attattagtt tctttgacaa cctttgcctt ctctgttgta aggcttgccc agtctaaacg
    12721 cataaaaacg gtttgaaggc cagacccttt tgaaatggca tccatgatag gcacaaagac
    12781 ctgtaatggc acaagtccgt ctttcttcgc ttgttcaatc aatttcatac gcagaacttt
    12841 tggatagttt tttccataac gatctgccaa tggtaggcca gttacatccg tgaaataaga
    12901 cacaacctct gccttttttg ttcgaccaac tggtttacca gctaaatctc tttggtaacc
    12961 atctacgaaa tagctcaatt caccaaggta gcctaacatt ttaccaacct ttatccctct
    13021 aaactcaacc tctaaatagg gaacaccttt tttaactacc aaagtcaccc gatgattaat
    13081 agccttgttt gacatggact cagatgctaa gtcagttttt aacatgctgg catttaaata
    13141 gtaaatacca tcacgcaatt gtttttgatt taatgctcta aggccgttct caactaaatc
    13201 tttctgatgt gaatgtggtc ctttcgccac aaggatatca ttagatgtca aatagatatg
    13261 ttctttttga gtagcaaatt tctttgttat taaagctttg cgaatccgat atgtacctgg
    13321 atcttttaaa gaaaactctc ccgttaagtc atctttcgga tcatttgaga gaagcttttc
    13381 gtcaaccgtc acatactgtc cattggaagc ttttttttgc aactgaatca caatactcca
    13441 atcgacttgc atatcattaa gtaaagaaac agtatctgaa cctttttctt gtaacaattg
    13501 ttgatattct tcaatagaca ctagttcttt atcaatggca gaatttgttt tttgctccca
    13561 ataatacaaa tctaaaagac tgaaagcttg atgatatttt attttttcgc catctaagga
    13621 agcttcaagt tttgcaatat gtttttgtgg agtcagtttg tgttgtccga taagagctct
    13681 tacttttgga ctcacacttt ttatctgatt gaacttcaca tctaaattaa gcgtttcaac
    13741 atctggaaat actgattctt caacctgagt aagcaagtta ttgaaaaaat caagttttgt
    13801 taattggtta ttctgtttaa atataccatt tggaagattt gaaatttcat tagttgataa
    13861 atcgagtaca gataatttag gaagattttt aaatgaaata ttatcaatac gagtaatatg
    13921 atttctactt gccacaatag tgcttaaggc gcgcaatttt tctattgttt ttggcagtag
    13981 cgctagatta ttttgagata agtcgataaa attaagtgat gttagaggct ctagcgcttt
    14041 ttcaggtatc tcctcaagac tattttctgc gaaacctaat gaagtcagac gagacaatcc
    14101 ttcaaatggg ttatcacaaa gtcggccaat acgattcttg gaaagatcaa gttgctccag
    14161 gtgatgaagc gactggaata gtcctccttc aagagagtga ataaagttac ttgataagta
    14221 gagttctctt aattgagcat tgcttttaaa tgtatctttg tctagataag ttaaatgatt
    14281 acttcttaag ttaagaaatc gaagttgctt catttgacta aataactctt ttggaatttc
    14341 cgtaatttgg tttccatcca gatgtagttc tgtaatattt ttagcatgtt tcaataaaga
    14401 aatatcagtt aactcgtaat ggtctagacg caactcacct cgaatctgtt ctaattctgt
    14461 ttttgataat ttaccatcgc cgtctaaatc tttgccaagt ttaactaaaa ctttttcaag
    14521 acgttcagtc ccaacccctt tttctttatc atcagtttct acctgataac cactccagcc
    14581 tttggtaacg gtcttactaa aggtcatgtc aagtttggtg tatttgtcat agtggtttaa
    14641 atcactttct tgacctccca tggctgatac caaaacagca cctttgtgta atttatccaa
    14701 atcatcaata ggcatggtaa attcgcttcg aatatagcta ccattgatat ctttttggcc
    14761 aacttgttta cgcactgctg ctgggtaggt ccctttgctt tcaatagaaa agtcaatcaa
    14821 aaattgtccc aaagcagtat taagcatgga aatttccatt gtaccatctg ctttaaccac
    14881 taatttggct cttttatcaa aagcaccttg aagcatggag gactcttctg agttttcttt
    14941 attagcttta aagtttagcg tataggttcc ttctgtaaga ttgttaatag aaacctcacc
    15001 tgcttgataa gactcgatgg tatcttttaa cagtcgatga gtatcggcga gtagcttact
    15061 atctttgatt tctttataag aatccttgat gacttgaagt ttctcagcca gatctttgac
    15121 cgcgtctttg ttgctttctt tagcaataat cgcatccgct tctttgatag ctttttccaa
    15181 agtggtgaca tccaactttg ttgattgtct gtcttcagca gtcacatgtt gtttttgttt
    15241 ggcagataca aaatggattt gaccatcttt cttggtaacg gtaatggtaa tgtcttcaaa
    15301 tccttttgag gatatcacga ttgtattttc tccatctttg aaagcttttg ttccaagatg
    15361 aagccctgtc atattcgaga cccagccatt agtatctttt acagaatctg aaataagatc
    15421 tttataaact gtaccattga ctgtaacctt ggtgatttgc tgagtcaatg cttttatctg
    15481 tttgttatca gaataacgat caatttctgg aaataaaatt gcaccatctt cttgactcaa
    15541 ttttgttatt aatgacaagt tagcagtatt tttcccagat ttaacaactt tttgtgtgcc
    15601 ttggttttgt ggcactggtt tatcactgag cacatctttt tttaatgaat cctgatgatc
    15661 tgttgttcct aatttttgag ctgaaataaa cgtcacttta tcgccctttt taataaaggt
    15721 aattaggata tcaccatctt ttttattaac aatagtcaat tcatgcttac cgtctttaat
    15781 aaggttagca ttgatcttaa tcccttggta gttaatatca aaggttcgct cgccttccag
    15841 gcttttttca tagacatcat tatcaacaat aaccttttca atatgtttat aatactcttt
    15901 tccagctgat tgactagcgt aagggcctat ctcttcaact aaaagtcgat tatcctgagt
    15961 taaagctact gttgtcacga gttgtgactg aactaaaggc tcttgtgatt ttacagtttg
    16021 agtagcctga atgagaacaa gggcagatat tgccacaaac gcacatttgc tgattttttt
    16081 cattttcaat caactttcta aaaattttta tgaataagct aactttttta ttaactgaac
    16141 ttaatctatc actttgtgat tattttgtca agattgtttc caatggtcca attgtctgtt
    16201 aaatagctcc actatgtgag ctaagacact agtaataagc ctggattgat aatgaggata
    16261 tctctgataa aagaattcct ctacaacact attactgaga taaagctagc cttaggatag
    16321 ttattactaa ggctgattca atcattactt cattcacaaa acatgtaaca cgttataagc
    16381 tccataattg tgttaggaga caacatgaga tgtcactact gcatattcat cacaataaaa
    16441 aaaagacaat agccctaatc gtgctattgt ctcaaaatca tttatttact tgaaacttta
    16501 tcgtgttaca ccaacagttt aaaaggccta actcccgcta agaaaattaa gccttaagaa
    16561 aagtcattag ttattagttt ttactcttgc caataacata tgttagctgt tcagcctgct
    16621 gagctgtaaa ggtacgataa gaaatcttgc ctaagccgat acagttagac tctgaaataa
    16681 gaatagaccc atcttttcta acatcttcta caattgatac gtggccataa gtgccatcag
    16741 cgcccgcttg ccctggtgca aatgaaatag cataacctac ttttggagtc tttgaaaggg
    16801 cataccctac tttatacttc caatctccac catttcccat aaaaggatca aattggtaac
    16861 ccaattcttt ggcgcgatta tacacatacc aagtgcattg tccccaagca taactcgaag
    16921 cttgataacc caagacatca taactcttag agagcttata acctgctggc acagatgcaa
    16981 aactagctga tgactgcact ttcaattcgg ctggaatagc tggtgtgcca ccatggtcat
    17041 caatccactt atccaggtct tccataattt gtgcgcgacg tttaccacta ccagtagtat
    17101 cagtaaaata aacacccccg tcacttgcaa agtttaattg acctcgtgaa aacttagccg
    17161 cttttaaatc ttgaagtgca aaattgctat ttttgttttt aataatggtg tcttgtgtca
    17221 ttttgacaat agcactatca tcattaaact tacttgcttg agtcggatct agatcaaaag
    17281 ctgcatagcc aaacatatta gctccaagca aagttgcaat cccttgagtt cctaaagaac
    17341 tctcagacat cgcaatagct acgatagctc gaacatccag cccacttttc ttttcccatt
    17401 gcaatagctt ctcaccatta atacgtgtgc tgtcataggc aatgcctgtt gattttaagt
    17461 aactatcaat ctggtcagct ttaataccat aacggcgaga taaaagatgg tgggtatagg
    17521 catttttacc actccaatgc tctacatatg ccgcatatgt gttgacagaa gggatatcta
    17581 tctcactcaa atctaaatct gatacgtaag gtgacaacga ctgaatggga gttcttgcct
    17641 ctggcagttg tttcgacaat gaagaagcag gtgccgtatc aactttagat ggtgatggtt
    17701 tgggctgatc agtcggtttt ggctggtcag tcggtttcga atgatcagtt gatttcgaat
    17761 gatcagttga tttatcatct acctggtttt ctgcttctgc cttgttgtcc gaagaaccta
    17821 cagcagactg actagaatcg cttctttttt tatcatccgc gttaacaaga ctattagagt
    17881 ttgtttctgc agaagcatca aggggacctt ggtcagtttt atctgatgtt gtaggtgctg
    17941 catttttatt attttcctct tttgaagaag aatcactggt ttcatttgta gttgctttat
    18001 gcgtctcaag cattacatct tgagccaaca ctgtcatgct ttctgctgtc ggggctaaga
    18061 gacttagtag catggcaact cttaatagtt tgtttttatc catatattaa ttagtcactt
    18121 ctttctaatg tcctcacttt attataacat taaagataag cacaatgcaa ttatttttac
    18181 atgattttct taatatatcc gaggaatacg gtcacttaaa aggcatagaa cttcataatt
    18241 gattgtatta cggtaatttg cgatatctgt tgtagaaata tttttttgct gattgctgcc
    18301 aatcaaagtg acttttgttc ctaaaggata tgctttggga agtcgtatgg tcagttgatc
    18361 catcgataca cgccctataa tttcacagaa ttgtccatca actaacaccg aaaagccttg
    18421 catgttcctg gtccagccat ctgcataacc gattgggacg gtccctacat attcagactt
    18481 tttggcagta taagtagctc cataaccgac tgtatcacca gctgaaatca tcttgacatg
    18541 cactaagcta gattctagag ataaagcctc ttgcagtgga aacggtaagc ttagatcaga
    18601 accacttgga ttcaaaccat aactgacaat tcctaaacga acagcattaa aaatggtatc
    18661 actatgccag atacttgtgg ctgaattact agcatgtact aaacgaggct tatcctcaag
    18721 tccagctatc agctttttaa aaaactgtaa ctgctggtta aatttagtat catctgcctc
    18781 atcagctgtg gcaaaatgag tgaaaatacc ttctacatca gcacccaact cttttagact
    18841 atcaatcaat aaattgactt cttttgaaga acgaagcccg atccgtccca taccagaatc
    18901 aacttttaca tgaactttta agccttgaca ttcttttttc tccagtctag ctaaagctat
    18961 ccagtctaaa ctagcgattg taacagtaat agcattagcg actgctaact ctaattcatt
    19021 tggcaggagg actccaagaa ttaaaatctc tttatcaata cctgcttgac gtaattgcaa
    19081 agcctcatca agatttgaca cacagtaccc atccacttga ggtaggagtg cttttgacac
    19141 ctggacagca ccatgaccat aagcatcagc cttgacaact gcatacgttt ttacccctag
    19201 tggaatatgc ttttgaacac tggcaacatt ctccttaata gcttgtaaat tgacccttgc
    19261 aactgttggg cgatggaaac ttgaaatcat tttttatcct ccaaaataac actagcctgt
    19321 acataattgc cactatgtga gatgctaata aaactatttc ctttaaacgg agattttgtc
    19381 agaatgggac atcctctaac atcatttaaa atctcaatat cttgaaaggt taaccgccca
    19441 atacctgtcc ctatagcctt agcaaaagct tccttacctg accagcgtcc tgccaaatag
    19501 ctgagtcgac gtttgtaagg gaaactctca aaaatagcca attcctgctc agttaaaatt
    19561 ttttgagcaa agcgaggatt tctttgataa actttttcaa tagctgaaat ctcttgtaag
    19621 tctattccat gtccaacaat cattactttc caccaaacct tatttcatct taatgttaga
    19681 agatttaaac agtgagaagc tgagaattat tctcagcttc tttctatata cctaactttg
    19741 tcctgttgtg tctcgttctt atgagaagga tttacgaccg tgacaatttt tgaatttttt
    19801 gcctgaacca catggacaag cttcgttacg ctccactttg ggtaaatcat cggtgttagc
    19861 tgactgctgc gattggatgt tttgaggtgc tgctgttgtg gcacgttgac ttgcacgttc
    19921 acgttcttgt tcgtgaattt gtgctttcat catggtacgt gttacatcaa actcgatagc
    19981 accaatcata tcttggaaca tcttgaaacc ttctgcttgg tattccacca cagggttatt
    20041 ttgagcatac cctcgaagtc caacagcatt acgaagttga tctaaggcat caatgtgttc
    20101 agtccatttg ttatccacaa tcatcaaaat gagtactttt tggaattcaa taatagcttc
    20161 ttgatcacga agttttgata gttgctggtc gtagatagca agcgctcttt gataaagctt
    20221 ctctttgatt tgttcgtctt ttaaaccacg taactcttta gcactgatag actcttctgg
    20281 aacaagtgac gtgcgtgcaa aggtcacaat ggcatccaca gcatccttac gattgctacg
    20341 tgcatgagca tctacagcac ggtcgatagt acgcttgatc atagctttaa tttctgggcc
    20401 aagatctcga ttagcagtta tgacatcacg acggttagca tagataattt cacgctgttc
    20461 acgcatcaca tcatcgtatt gtaagacctg tttacgggta tcgtagttat ttccttcaac
    20521 ccgtttttga gcagattcca cttgacgccc taacatacct gatttaataa cagtgtcttc
    20581 ttcatctagt ttcatccgat ccaaaaaggc ttttatacga tcagagccga aacgtctcat
    20641 caaatcatct tctaatgaaa gatagaattg ggattcacct ggatctcctt gacgacctga
    20701 acgtccacga agctggttat cgatacgacg gctttcatga cgctctgtac caatgacaca
    20761 taacccacca agttcacgaa caccttcacc aagcttgata tcagtaccac gaccagccat
    20821 gttagttgcg attgtaacag caccacgttg cccagcattc ataatgattt gagcttcttt
    20881 aaagtgattt ttagcattca aaacctcatg aggaatccct gcttcaacca gcttgcgtga
    20941 aatcaaatca ctagtttcaa cagcaaccgt acctacaagg ataggttgtc cttttgcatg
    21001 acgcgtttta acgtcttcca caactgctct aaactttgat tctaaggtcg ggtaaagcaa
    21061 atcagtgtga tcaatacggg caattggacg attggttgga attggaataa tacgcatgtt
    21121 ataaacttcg cgaaattctt cttcctcggt cttcgcagta ccagtcatac ctgccaattt
    21181 tttgtacata cgaaacatat tttggtaggt gattgaggcg ctggtttttg attcttcctg
    21241 aatacgaacg ccttcttttg cctcaatagc ttggtgcaag ccgtctgaga agcgacgccc
    21301 ttccatcgta cgacctgtaa attggtcaac aataagaatc tcaccatctt cgttgaccac
    21361 ataatcgata tctaacaaca taatatagtt tgcacgaagg gcgttgtcta taaagtgagt
    21421 caaggctaca ttttcaatat cgtaaagatt gctcaagtta aaataactct cagctttatc
    21481 aatacctgaa tcgcttaatc caatcgtttt agtgggcaca tcaataacat aatccactga
    21541 cgtcagagtt tttacaaaca tgtcagcacg aatatacaat tgattggttt cagagctaac
    21601 agcaccagaa acaattaaag gtgtccttgc ctcgtcaatt aagactgagt ccacttcatc
    21661 aaccaaggca aaattgagtg ggcgttgtac catgtcttcc tggcgaacaa ccatgttgtc
    21721 acgaaggtaa tcaaaaccga cttcagagtt ggttgaataa gtaatatcac aattataagc
    21781 ttcacgtttt tcagcaggag acttagcagc taggttgatc ccaactgaaa gacctaacca
    21841 gctatacact tcacccattt cggtagcgtc acgcgttgaa agatactcat taacagtaat
    21901 aacgtgaaca ccctcaccag caatagcatt gagataaact ggcatggtgg ctgtaagggt
    21961 ttttccttca ccagtacgca tttcagggac atccccgtta tgaagaacaa ttcctcccat
    22021 aatttgaaca cgataaggga acaagcccaa cacacgtttt gctgcttcac gaacaactgc
    22081 gaaagcttca ggtaatagtt gttcgagggt ctcacctttt tgatagcgtt ctttaaattc
    22141 aagtgttttt ccttgtagat ctctatccga caatgacgcc atttggtctg cgtaggattc
    22201 tacttttttt gcaatttttt ctaattttct tagttcgcct ttgtcatttt cgataacttt
    22261 gcgtagaata ttggccattc tctttccttt cgatcatcta atcatttcat tttaacataa
    22321 atatgcaggc agttcaagaa aggttggtaa agtt
//
LOCUS       DATURV010000024        22309 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DATURV010000024 DATURV010000000
VERSION     DATURV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22309
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.024"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            79..333
                     /locus_tag="VCF75_000898"
     CDS             79..333
                     /locus_tag="VCF75_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEP2546475.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            351..746
                     /locus_tag="VCF75_000899"
     CDS             351..746
                     /locus_tag="VCF75_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEP2546476.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVERVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            1031..1350
                     /locus_tag="VCF75_000900"
                     /pseudo
     CDS             1031..1350
                     /locus_tag="VCF75_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264372.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1347..1910
                     /locus_tag="VCF75_000901"
     CDS             1347..1910
                     /locus_tag="VCF75_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2546477.1"
                     /translation="MNLIIIGAQASGKMTIGQEVARQTGMTLFHNHDSIDFVLRFMPW
                     SQESTALIERIRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTQLTAAETAQLIIQKMTHIKEN"
     gene            1912..4560
                     /locus_tag="VCF75_000902"
     CDS             1912..4560
                     /locus_tag="VCF75_000902"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEP2546478.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKCVHSVGHSERSGAAVEPRLSTQWFVKMDELAKQAMDNQETDDRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIL
                     RLLHPIMPFVTEEIYAQYAQGSIVTAVYPTVTPTFENEAAHKGVESLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVVQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            4714..7122
                     /locus_tag="VCF75_000903"
     CDS             4714..7122
                     /locus_tag="VCF75_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEP2546479.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWNYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSIVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDMTDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            7255..7983
                     /gene="cas5c"
                     /locus_tag="VCF75_000904"
     CDS             7255..7983
                     /gene="cas5c"
                     /locus_tag="VCF75_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEP2546480.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            7983..9878
                     /gene="cas8c"
                     /locus_tag="VCF75_000905"
     CDS             7983..9878
                     /gene="cas8c"
                     /locus_tag="VCF75_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEP2546481.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNESVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QTQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANREKLGI
                     CNISGREEQLTDKHRGLMGNTKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYHKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            9883..10731
                     /gene="cas7c"
                     /locus_tag="VCF75_000906"
     CDS             9883..10731
                     /gene="cas7c"
                     /locus_tag="VCF75_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEP2546482.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKPIFVQANERIEDDFRSLERRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYHQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            10733..11407
                     /gene="cas4"
                     /locus_tag="VCF75_000907"
     CDS             10733..11407
                     /gene="cas4"
                     /locus_tag="VCF75_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEP2546483.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQI
                     LHTKADNPYIKEKRKDLLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMHEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            11404..12429
                     /gene="cas1c"
                     /locus_tag="VCF75_000908"
     CDS             11404..12429
                     /gene="cas1c"
                     /locus_tag="VCF75_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEP2546484.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGRASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILMTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            12440..12733
                     /gene="cas2"
                     /locus_tag="VCF75_000909"
     CDS             12440..12733
                     /gene="cas2"
                     /locus_tag="VCF75_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEP2546485.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVNYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   12882..13776
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12882..12913
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaat"
     gene            13936..14646
                     /gene="ccdA2"
                     /locus_tag="VCF75_000910"
     CDS             13936..14646
                     /gene="ccdA2"
                     /locus_tag="VCF75_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEP2546486.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNIFYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHILLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            14659..15282
                     /locus_tag="VCF75_000911"
     CDS             14659..15282
                     /locus_tag="VCF75_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEP2546487.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFKKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            15325..16431
                     /gene="msrB"
                     /locus_tag="VCF75_000912"
     CDS             15325..16431
                     /gene="msrB"
                     /locus_tag="VCF75_000912"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP2546488.1"
                     /translation="MEHKWKLWLFIVGVVFLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKVIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVTYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSAAEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVIRYKEDKSFNMTRTEVRSRSGDSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            16519..17259
                     /locus_tag="VCF75_000913"
     CDS             16519..17259
                     /locus_tag="VCF75_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEP2546489.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLRKKLELSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKELGMPFQDYLIQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLIGVTPSQYKKGDQA"
     gene            17256..18989
                     /locus_tag="VCF75_000914"
     CDS             17256..18989
                     /locus_tag="VCF75_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2546490.1"
                     /translation="MKRYPLLVQLISYIFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNSQLIMKTSSDMMAEPWYKAAIDRQAMPILTPARQLSLSS
                     KKEWVVSVTQEVVDAAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIKNGYAKDKSSFVYQRVIPNSQWTLIGVASLDQLHRV
                     QRQIFWSFLGMGLLACLICGLTTILVLRKWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYD
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLMLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDHFTEIQLTLPKMH
                     ELMADDTQENE"
     gene            19062..20141
                     /locus_tag="VCF75_000915"
     CDS             19062..20141
                     /locus_tag="VCF75_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEP2546491.1"
                     /translation="MSLASDYFSRQTPIVEKLVAYGFEKRDNGYFYNERFMEGEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLGRLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKAFPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETNIPNEGYRKNPNIDKLMRLRKRQQYKDGLLSFDLMKEHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            20155..20871
                     /locus_tag="VCF75_000916"
     CDS             20155..20871
                     /locus_tag="VCF75_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEP2546492.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVLLFTEN"
     gene            complement(21037..21510)
                     /locus_tag="VCF75_000917"
     CDS             complement(21037..21510)
                     /locus_tag="VCF75_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP2546493.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATICGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            21652..>22309
                     /locus_tag="VCF75_000918"
     CDS             21652..>22309
                     /locus_tag="VCF75_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP2546494.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     D"
BASE COUNT         6984 a         3990 c         4844 g         6491 t
ORIGIN      
        1 cagttattaa taacatggca caagaggcct gccttatggt ataatggtaa agataatttc
       61 agaaagagga agccaagcat gtcttatgaa aaagaatttt taaaagattt tgaagactgg
      121 gttaaaactc agattcaagt caatcagcta gccatggcaa ccagtcaaga agttgcccaa
      181 gaagatggcg atgaaagggc taaagatgcc tttattcgtt atgaaagtaa gttagacgcc
      241 tacgaatttt tactaggtaa atttgacaat tataaaaatg gtaaagcctt tcacgatatt
      301 ccagatgagt tatttggagc aaggcattat taagaaggag acacgaaata gtggcaaaac
      361 aaaaaaatcg taaaaaagca ttgaaattag aaatgaagcg taatggcttg ttaaaaaaag
      421 caggtcaagt ttttgataag gcagttgaaa gggttgaaac agctgtggat aaaaccattt
      481 ctgctggtaa aaatcttgtg gaaaaaggca gccaaacggt tgaaaacctg acagcaagca
      541 aggaaagact tactcttgat gagtttgcag ggcagccagt tgtagctggt atccgctctg
      601 accttgtcga aaccctatat gcagaaggca ttcattcagc gcaagctttt aaggagtgga
      661 cagaaaaaga cctccttgct cttaagggca ttggcccagc aacggttaaa aaattggtcg
      721 aaaatggcgc aagctttaaa aaatagtcat cttctcgttg cttaatggct ataaatttgt
      781 taaaatatta gcaatcagag gtgcattgag ttggtctgtt acagagagcg gtggtgttga
      841 gaaatccgca cagatgtcaa ggctagtagc tgaggatcag aaatcagata tgaaaagtag
      901 cagacccatt ttatggtttg aaacagtggt ggtaccgcgg aaatcgtaac acgaaattcg
      961 tccctgtcta ggttatctag gcagggatta tttggtttca tttgaaagaa gatgatggct
     1021 tattgttaaa atgttgttag aagtatataa tagaaaaact ttgcccaaag tggaaacaga
     1081 acggctgcta ttacgtcaac gaaaagggga caaggcattg ttccagaagc ggcttcagct
     1141 ctgattgaaa ttggttttac cttacttgat ttgcataaaa ttgagctggg ttgctacgac
     1201 tacaacaagc aaagtcaggc agtggctcgc aaattaggct ttaccttaga agccaatgcc
     1261 cgtgaccgta aggacgttca aggtcgccgc tgtggcgaca tgcgttttgg gttactgaga
     1321 agtgagtggg aggagcagaa acaaaaatga accttatcat cattggtgct caagcgtcgg
     1381 gcaagatgac cattgggcaa gaagttgcca ggcagacagg catgactttg tttcataatc
     1441 acgattctat tgactttgtt ttacggttta tgccttggtc tcaagagagc actgccttga
     1501 ttgaacgtat ccgttttgct ttttttgaaa cctttgctaa gacaggtcaa gacatgattt
     1561 ttactattgt aattgacttc aatgatccaa atgatgtagc aatgcttgaa aagattcagg
     1621 ctgtttttca gtcttatgac caagaggttc tctttgtcga attaaaaaca gatatagagg
     1681 aacgtcttaa acgcaatcgc acagaaaatc gtctcaaaca taaacctctt aaacgcaaca
     1741 tcgagtggtc agaacaagat attcaatcca cgatggcata tgctgttttt aatccagaag
     1801 aaccacccaa aacgctcacg cattaccaaa aaatcaataa tacccagctc accgctgctg
     1861 aaacagcgca gcttattatc caaaaaatga ctcatatcaa ggagaactaa gatgacagaa
     1921 ctatcaccta aatacaatcc tgccgaggtt gaggcaggtc gctatcaaaa atggcttgat
     1981 gcagatgttt tcaagccatc aggagaccaa aaagcaaaac cgtattctat cgtgattcca
     2041 ccgccaaatg tgactggtaa gttgcacttg ggacatgctt gggacacaac gcttcaagat
     2101 attatcattc gtcaaaaacg catgcagggc tttgatacgc tttggttgcc agggatggac
     2161 catgcaggga ttgcgacaca ggctaaagta gaggagcgtc tgcgcgagca ggggatttct
     2221 cgttacgacc ttggtcgtga caaattccta gacaaggtct gggagtggaa agacgaatat
     2281 gccactacga ttaaagagca gtggggcaaa atggggcttt ctgtggacta cagccgtgag
     2341 cgcttcaccc ttgatgaggg cttgtcaaaa gcagttcgca aggtttttgt tgacctctac
     2401 aaaaaaggct ggatttaccg tggtgaattc atcatcaact gggatcctgc tgccagaact
     2461 gccctttctg acatcgaggt tatccacaag gatgttgagg gagctttcta tcacatgaac
     2521 tatatgttgg aagatggctc acgcgccctt caagtggcaa caacgcgacc agaaaccatg
     2581 tttggagacg ttgccgttgc cgtaaatcca gaagatcctc gttacaaaga tttgattggc
     2641 aaaaatgtta ttttaccaat cgtgaataaa ttgattccaa tcgtgggtga cgagcatgcc
     2701 gatcctgaat ttgggactgg agttgtgaaa atcacgcctg cccatgatcc taacgacttc
     2761 gaggttggtc agcgtcataa cttaccacaa gtcaatgtca tgaacgatga cggtaccatg
     2821 aatgagttag ctggcgactt tgcaggcatg gaccgttttg aagctcgtca agctaccgtt
     2881 gctaaacttg aagaacttgg cgccctagta aacatcgaaa aatgtgttca ctcagtaggt
     2941 cactcagaac gttcaggtgc agccgttgag cctcgtctat ccacccaatg gtttgtaaaa
     3001 atggatgagc ttgctaaaca agctatggac aaccaagaaa cagatgaccg agtagatttc
     3061 tacccaccac gctttaatga taccttcctt caatggatgg aaaatgtcca tgactgggta
     3121 atctcgcgtc agctttggtg ggggcaccaa attcctgctt ggtacaatgc agaaggtgag
     3181 atttacgttg gcgaagaagc accagaaggt gatggctgga ctcaagatga agatgttctt
     3241 gacacttggt tctcatctgc tttatggccg ttctcaacta tggggtggcc tgacacagat
     3301 gtagaagatt tcaaacgtta tttcccaacc tcaaccttag taacaggtta tgacatcatt
     3361 ttcttctggg tgtcacgtat gattttccaa tcgcttgaat ttaccgggcg tcagccattc
     3421 caaaatgtcc tgattcacgg tcttattcgt gacgaagaag gacgcaaaat gtctaaatca
     3481 ttggggaacg ggattgatcc tatggatgtc attgagaagt acggtgccga tagcttacgt
     3541 tggttcctct caaacggctc tgccccagga caagacgtgc gtttctcata cgaaaaaatg
     3601 gacgcctctt ggaatttcat taataaaatt tggaacattt ctcgctatat cctcatgaat
     3661 aatgaagggt tgaccttgga agaagctgaa agtaatgtgg ctaaggtagc tgcctcagaa
     3721 gctggaaatg tgactgacca gtggattctc cacaatctca atgaaaccat cgctaaagtg
     3781 acagaaaact ttgacaagtt tgagtttggt gtggcaggac acattcttta caactttatt
     3841 tgggaagaat ttgccaactg gtatgtggaa ttgaccaagg aagtgctgta tagcgacaac
     3901 gaagctgaga aagttatcac gcgctctgtc cttctttata ctttggacaa aatcttgcgt
     3961 ctccttcacc caatcatgcc atttgtaacc gaagaaatct atgcccaata tgcccaaggt
     4021 tctattgtga ccgcagtata ccctacggtt actcctactt ttgaaaatga ggctgcgcat
     4081 aaaggtgtgg aaagtctgaa agatcttatc cgtgctgtac gaaatgcgcg cgctgaagtt
     4141 aacgtggcac catcaaaacc aatcaccatt ttggtgaaga cagcagatag tgagttggaa
     4201 gacttcttca ccagcaacgt gaactacatc aaacgtttca ccaatcctga aaaattagaa
     4261 atcagctcag ccattgctgc gccagagctg gctatgacca gcatcatcac aggtgctgaa
     4321 atctacttgc cactagcaga tttgcttaac gtggaagaag aactcgctcg cctagataaa
     4381 gaactggcta aatggcaaaa agaattggac atggtcggca aaaaactcgg aaacgaacgc
     4441 tttgtagcca acgccaaacc agaagtcgtc caaaaagaaa aagacaaaca agccgactac
     4501 caagccaaat acgacgcaac ccaagaacgg attgcagaga tgaagaaaat taaatcatag
     4561 tgtttagaac gcagttgaaa aactgtgttc ttttcttact aatagagata ttagattgaa
     4621 gtttaattat gagaaaaacg ctttctttat ttaaagagtt atgttacaat actattacaa
     4681 tcatcgtaca aatagtgtta ttttgagggg catatgagaa tgattttagc acactatgac
     4741 tgtaaaaaag ataaaaagca atctttagat gagcatttat ggcatgtggc ctgttctagt
     4801 cgacaggaag catctataat tggtcaagga gatgtgcttt ttttaattgg tctttaccac
     4861 gacctgggca aagctgatcg aacctttcaa gataaattat taaataatcc aaatcggcat
     4921 gttgatcact cttatgcagg ggcaaaatac ttatgttcta ttattgggcc tcatctaaaa
     4981 aaccgagggg ttgataaaaa tgagagaatg acattcaacg aaatggtggg gtatgtcatc
     5041 tctgctcatc atgggatgta tgatttatgc tactattttg acgatgctga atattatggc
     5101 tttaataagt ttaaaaatcg tatcaataga gacttagatg gttatcacta tcatgaagat
     5161 attaaagggt acgctctaaa attagaaaaa aaattatgtg attatggcta caaagattta
     5221 agggagctta ttgataaagc ttttgataat taccaacaag ccatgtcttc cttaaactgg
     5281 caagataaga gtgagtggaa ttattatcag tcttgtatgg tgagacttta cttgtcactc
     5341 ttaaaaaacg ctgatatttt ggacacagta aatgcctatg gccttaagat aagtcctatg
     5401 gataaaacag agcgatcctt tctaaaacac tcctatttag cggccattga acaaaaatat
     5461 gctagctttg gacagccaaa caatcagttg aacactattc ggacagaaat cgctgagcgt
     5521 gttaaagaaa gaggtaaacg agattccaag gggatttatc gcttagattt accgacagga
     5581 gctggcaaga ctaatcttag tatgcgttat gcgtttcacc aattagttca tcacgacaaa
     5641 tcaaggtttt tttacataac gccctttctt tcggttcttg agcaaaatgc ttccgaaatt
     5701 agaaaagtta caggtgacct tggcgttcta gaacaccatt ccaatgtggt gaaacaggct
     5761 aatgaagatg atgatgacaa ggacagttta ttgtcagctt atcttagtga tagctgggac
     5821 agtcaagtag tcttgacttc tatggttcaa tttttccaaa cacttttcaa aacaaaatca
     5881 gctaatctga gacgtttttc aagtttgatt aatagtattg tgattctaga tgaagttcaa
     5941 tccctgccta ttgaagtcac cactttgttt aatttaacga tgaatttttt aaataaagtt
     6001 atggatacaa ccatcgttct ttgcacagcg acacaacctg cttatgattc ttcagagatt
     6061 gaccatcgta tctgttatgg agggaacttg ggagaattag ctgaaatcgt tgagttaacg
     6121 attgaagaaa aacagatttt ttcaaggaca gagcttagaa aatttgatga tagtgatcag
     6181 aaagttcact tgactgatgt tattaacctt attctaggtg aggaaaactc cgttcttgct
     6241 atttttaata cgaaaaaaac ggttcataac tgctatacta tgctaaaaga catgactgat
     6301 agaccggtct atcagctttc gacaaatatg tgtgcgcagc atagacttga cttgattgct
     6361 aagatcaaaa cggagttaca aaataatatc cctattattt gtattagcac gcaattaatt
     6421 gaagcaggtg tagatgttga ttttcatcgc gtcattcgtt cctactcagg gattgattct
     6481 attgttcagg ctgctggacg gtgtaaccga gaaggcaaac gagataaagg gcaagtcact
     6541 cttgtcaatc tgaccaatga agaggaaaat atttctaggc tgacagaaat aaaagctaaa
     6601 aaagaagcca cagaatctat tcttcataag attgggtctc caattgatat ctcaacttta
     6661 aaccgtgact tttttgagta ttattatgcc aataatcagg gactgatgga ttatcctttg
     6721 gaagacaacc tatcaatcta cgactattta agccttaata tttatcagac ggcaaataaa
     6781 aagttcaaag gtaagttaaa acaagctttt aaaacagcag gagccaaaat gaacctcatc
     6841 aataatgata tgataggaat tctcgtacct tatggcgaag ctgagaaaaa attggcttat
     6901 ttagaagaat tgggtatgtc tcacttttta tcagcagaag attatcaaac gataaaatca
     6961 ttactaaaag agttacaacc ttttacggtt aatgtccgcg agaacgatcc tctctttgag
     7021 acaacaaaat cttatctaaa tggtcagatt ctggttttga cgtcggagta ttatgacacg
     7081 gaaagaggag ttaaatacga ttcagctagc ttttacttct aactcaaaac gaaagcagat
     7141 taacaaaagg ttgttagagg accttgttaa cctgccaatc atcattaata attattatca
     7201 atttagacta tttaataaaa ttagattaca aaaaaacaga aggaggaaag tagcttgtac
     7261 agatctagag acttctacgt gagagtaagt ggtcagcgag ctctttttac aaatccagcc
     7321 acaaaagggg gatcggaacg ctcatcctat tcggttccga ctagacaggc actgaatggt
     7381 atcgttgatg ccatctatta taagccgacc tttactaata tcgtcacaga ggttaaggtt
     7441 attaaccaga ttcaaaccga attacagggt gtcagggctc tgttgcatga ttatagtgca
     7501 gatttaagtt atgtatccta tttgagtgat gttgtttatc tgatcaagtt tcattttgtt
     7561 tggaatgaag atagaaaaga tttgaactca gatagacttc cagctaaaca tgaagccatt
     7621 atggagcgtt ctattcgtaa agggggacgt cgagatgtgt ttttgggtac aagagaatgt
     7681 ttagggcttg tagatgatat cagccaagaa gagtatgaga ctactgtgtc gtattataat
     7741 ggtgtcaata tcgacttggg aatcatgttc cattcctttg cctatccgaa ggacaaaaag
     7801 acaccattaa aatcatactt tacaaagact gtgatgaaaa atggagtcat tacgtttaaa
     7861 gcacagtctg aatgcgatat tgttaacacg ctttctagtt atgcttttaa agcaccagag
     7921 gagataaaat cggttaacga tgaatgcatg gagtatgatg ccatggagaa aggagaaaac
     7981 tgatggattt ttttacttct ctcttgaaga cttatgaaaa agcagagcta gcagacttgg
     8041 ttgatcacca aaaaagaagt aatgagtcgg ttttactgcc gatttatcat acgagtttaa
     8101 agtcaaatgg caaaaatatc atttcagtga aacttgacaa agatggccag tttcacaagg
     8161 cagaatttat ggcagataag caaatgatta tttttcctgt aacggctgat tctgttgcta
     8221 ggtcaggtag tcatcctgca ccgcatcccc tagtcgataa atttgcttat tatagtgctg
     8281 aaatggggca gactcagtat gattcttttc ataagcaact taataactgg attgattatt
     8341 gtgaggaggg tgacgtcaag aggtttttaa ccattgttca gcaatttatt ttgaaaccag
     8401 aatttctgac actgattctc gattctttaa ttggtcctga ttatcaaaat aatcaattaa
     8461 aagtcacatt ttgtgatgcc actggaaaag aaaaattaat tgatttatca gcttgctttt
     8521 tagaattttc aattgatcag ttccagggct ttaaaaatga atcggtttcg acatttaaaa
     8581 ctttacacca atcctatatt tcttttgttg aagccaatcg tgaaaagctt ggtatttgta
     8641 atattagtgg acgagaggaa cagctcaccg ataagcatag aggtttgatg gggaatacta
     8701 aaatcatctc tgttagtaat aaaagagaag cttataaagg acgttttaga gaacgcgaag
     8761 acgtttttag tgttggctat gaaacttccg aaaagattca tttaatgctc aagtaccttt
     8821 tagaaaataa aaataccagt acttggttag ggtcttctca atatttaatc aactggttta
     8881 gtgatgattt aacaaatgat agtcggttgg atatcgtatc accaatcttt gatgacggac
     8941 ttgaagaaga tgatgatgac gatacgcctc ctgttataac attagcaact gaagacaata
     9001 aaagaattgg taaatcattc atcaagggac aaaaattatt tgctaatgat gccacttact
     9061 acgttgctat tttgaataaa accagcaatg ggcggattgc tttaaaatat tttcgtcagc
     9121 ttcaagcgtc ccaattactc accaatctta acaagtggca ggaaacatac agttgggagt
     9181 cgcgatctaa gtttggaaaa agtcgcttaa gaacccctac ttttcatgac atccttaatg
     9241 tgtcctacgg ggttgatagg gatcgcttcc ttgaattaga taatgataac ttcaaaagtg
     9301 atcaaattca aaagttagtg gcaagtttga ttgatggtaa accgatgcca cagtccattg
     9361 tcaaaaagtt aggtaacaat gttaaagaac gacatcgtta ccataagcac tggtatcaag
     9421 ttgagcaggt ctgcttagca attttacaca aacaaaatgg ggaggaattt tcaccgatgc
     9481 tagatcatac caatcaaaat cgttcctatc tttttggacg attattagca atttttgaat
     9541 taatcgagac cttgcgttat ggcttggatg gaaacaataa cgaccgtatt accaatgctg
     9601 aacgttattg gacagcctat actggacaac caacaaaatt gatgatgtta ttggaaaata
     9661 aaattaagcc ttacgaagaa ccattgaaat taaatcgtcg tggcagttgg atgaaattag
     9721 aaaaagaaaa agaagagatt ttagaactgt taaatcctct gttagagaca gaaacaatgg
     9781 aaaaaccctt agattaccgc tttatttttg ggtattatgc tgagaaaaac tattactata
     9841 caaaacaaaa cactgaagta acagaaagtg aggagtaaaa agatgttgga acacaaaatt
     9901 gattttatgg taactcttga agtgaaagaa gcaaatgcaa atggtgatcc cttaaatgga
     9961 aatatgcctc gtacagatgc caaaggatat ggtgtgatga gtgatgtctc tatcaaacgt
    10021 aagattcgta atcgtctaca agatatgggg aaacctattt ttgtgcaagc taatgagcgt
    10081 attgaagatg actttcgttc actagaaaga cgcttttcgc aacattttac agctaagaca
    10141 cctgacaaag aaattgaaga aaaagcaaat gcattatggt ttgatgttcg tgcttttgga
    10201 caagttttta cttatctgaa aaaatcaatt ggggtgcgtg gaccagtttc catcagtatg
    10261 gctaagtcct tggagccaat tgtcatttcc agccttcaaa ttacgcgtag taccaatggt
    10321 atggaagcta agaataatag tggccgctct tctgatacga tggggacaaa acattttgta
    10381 gattatggtg tgtatgtact taaaggttct atcaatgctt attttgctga aaagactggt
    10441 ttttctcagg aagatgctga ggctattaaa gaagttttgg ttagcttgtt tgaaaatgat
    10501 gcgtcgtctg cacgtccgga aggctctatg cgagtttgtg aagtcttttg gtttacgcat
    10561 tcaagcaaac tggggaatgt ttcaagtgcg cgtgtctttg acttgttaga gtatcatcaa
    10621 tcaatagaag aaaaaagcac ttatgacgct tatcagattc atctaaatca agaaaaattg
    10681 gctaaatatg aagcgaaagg gttaacggtt gaaatcctag aaggactcta gtatggtcta
    10741 tgccgaagat gattatttaa tgctatcagg tattcagcat ttccaatttt gtaaacgtca
    10801 atgggcgttg atccatattg agcaacaatg gcttgataat gaagcgacag cgcatggaca
    10861 aattctacat actaaagcag ataatcctta cattaaagaa aaacgaaaag atcttttggt
    10921 ctcacgtgct atgcccattt cttctgcaga acttggactt tcaggaatta tggatgttgt
    10981 ggaattttat aaagatgatc aaggtgtgtc tttgagggga aaacgtggga aatggttacc
    11041 aaaagttgtg gaatacaagc gcggaaaacc taaaaaagat accagagata ttgtccagtt
    11101 ggtggctcag accatgtgtt tagaagaaac gctagactgc gacattaacg aaggttgtct
    11161 ttattaccat agtgtcaatc aaagagtgat tgttcctatg acatcagctt tgcgtcaaga
    11221 agtgaaggaa ttagccgcag agatgcatga ggtttatcag agtcaaatgc tacctaaagc
    11281 agcttatttt aaaaactgtc agctttgttc tttagtcgat atttgtaagc ccaggttgag
    11341 taaaaaaaca aggagtgtgt cgcgttacat caatgaggct atgaccagtg aggagatgga
    11401 cctatgaaga agttgctaaa taccttgtat ttgacgcaag aagattttta tgtcactaaa
    11461 gagggcgata acattgttat caagcaagaa ggtaaggttc tcaaacggtt tccgtttcgg
    11521 attattgacg gtattgtctg tttttcttat ttgggtgtgt cgcctgcttt ggtgaagtta
    11581 tgtacggaga atcagattaa tttatcgttt catacaccac aagggcgttt ttgtggtcgc
    11641 tatattggtt caaccaatgg gaatgtgttg ttgcgtagag aacattatcg tttatctgat
    11701 cgtgaggaat ctttggaata cgcaaagcgg tttattttgg ctaaaatttc caattcaagg
    11761 aaatacttgc tacgctttaa acgagatcat cgtcaacaga ttgataccaa gctttttgag
    11821 gctgttaatg acgaattgat atgggcttta gagatggttc aggcagcaga taataaagac
    11881 tctttaagag ggattgaagg ccaagctgct aatcagtatt ttcgcatatt taatgacctg
    11941 gtgttgacgg acaaaaaaac gttttacttc caaggtcgga gtaaacgacc acccttagat
    12001 tgtgttaatg ccctcttgtc ttttggttac agtttactga cctttgaatg tcaatctgcc
    12061 ttggaagctg tcggattaga cagttacgtt ggtttctttc acacggatcg tcctgggcgt
    12121 gctagtttag cgcttgattt agttgaagag ttccgctcat atattgtaga tcgttttgtc
    12181 ttttcattaa ttaataaagg acaacttcag aaaaaacact ttgaggttaa agaaaatggt
    12241 agtattttaa tgacggaaaa tggcagagct atttttattg atttgtggca gaagcgtaag
    12301 catactgagg tagaacatcc ttttacaaaa gagaaagtaa aacttatgtt attaccctat
    12361 gtacaagcgc agcttttagc taaggccata cgaggagatt tagaaagcta tccacctttt
    12421 atggtttagg agatgttata tgatggtttt agtcacttat gatgtaaata cggaaacacc
    12481 tgctggtaga aaaagattgc gtcatgttgc caaactctgt gtgaactatg gacaacgtgt
    12541 tcaaaattct gtttttgaat gttctgtgac acccgcagaa tttgtggata taaagcaccg
    12601 cttaacacaa atcattgatg agaaaactga tagtattcgc ttttatttat tggggaaaaa
    12661 ttggcagagg cgtgtggaaa cacttggtcg ctcagacagc tatgacccag ataaaggtgt
    12721 cttattattg taaaaatctc ttgtgcgaag ctagctttca cagaaacacc ttgcttgctc
    12781 gcgcaaaaat aacttaaaaa agaagcgaaa tggagataaa aaggcttaaa acacctctat
    12841 gtgtgccatc cgtttctcta taaactgtgt catttggcgc agtctcaccc ttcatgggtg
    12901 agtggattga aataagctaa acctatccta aaaatcaaag gcacagggtc tcacccttca
    12961 tgggtgagtg gattgaaata atgactatta tggggatatc tgccacgata tagtctcacc
    13021 cttcatgggt gagtggattg aaataccaat aattctaaag tcgctatctc tatctacggt
    13081 ctcacccttc atgggtgagt ggattgaaat ttataataca accaatagac ataatctaga
    13141 atcgtctcac ccttcatggg tgagtggatt gaaataaact atgtgactga cactctggac
    13201 tatgtcaaat acgtctcacc cttcatgggt gagtggattg aaattgctgt aggtaaagcc
    13261 gataataact ggggcggagt ctcacccttc atgggtgagt ggattgaaat aaaaaaatag
    13321 ctaagatcgc tactgtgtct gctgtctcac ccttcatggg tgagtggatt gaaatatgta
    13381 atcctgttgt taacttgtca aagtcataga gtctcaccct tcatgggtga gtggattgaa
    13441 ataaaaacaa atcgttatca atttgcgtct ttccagcgtc tcacccttca tgggtgagtg
    13501 gattgaaatg attcccccac gaagcaaaca agaattcaca gtcgtctcac ccttcatggg
    13561 tgagtggatt gaaatacgta aaatattaat cagtgttgat ttaccagtaa ccgtctcact
    13621 tttcatgggt gagtggattg aaataaattt ggcgaggaga gcgtgaggcg aatctagtgt
    13681 ctcacccttc atgggtgagt ggattgaaat attagcactg gttatattaa gtgccgagga
    13741 aaaagtctca cccttcatgg gtgagtggat tgaaagatga acccgacctc tcttttaatg
    13801 aggtcgggtt tttacatgcc tttagtcaac catttatctg atagcgtatc aaattcaagt
    13861 ttttgcatgt tctagtctat ggtttgcgcc actagctttc ggtaaactaa gagtaactta
    13921 agagaggagt tatttatgac actatcactt gtattaatgg tatcggtttt tggggcaggc
    13981 ttgttatcat ttttctctcc gtgtatcttt ccggtccttc ctgtctattt aggcatttta
    14041 ctggatgcag atgattcaaa gacgattact atatttggta aaaaactcta ttggtatggc
    14101 attgtcaaaa ccttagcctt tatttttggt ctatctacta tttttgtgat tttaggttac
    14161 ggggctggtt ttttaggaaa tatcttttat gcggtatggt ttcggtactt actgggagcc
    14221 ttggtcatta tcttggggat tcatcagatg ggcctcatta ccattaagag cttacaattt
    14281 caaaaatcac tgactttcca taataacaag aatcgcaacg gtttgttcaa tgcatttatc
    14341 cttggtttaa cctttagctt tggttggacg ccttgtgtgg gacctgtttt gagttctgtt
    14401 ctagctttgg tggcttcagg gggaaatggt gcctggcaag gtggcgtttt gatgattatt
    14461 tatactcttg gattgggcat tcctttcctg cttatctctt ttgcgtcagg cattgttttg
    14521 aaacagttta acaagctcaa accccacata cttttactga aaaaagtagg aggcgttctg
    14581 attatagtca tgggaatctt gcttatgaca ggaaccttaa ataacttagc acaacttttt
    14641 ggataaagga gaaacacaat gaaaaaagga ctattagtaa caactggttt ggcttgtctc
    14701 gggctactaa ctgcttgctc aacccaagac aatatggcta aaaaggaaat aactcaggac
    14761 aagatgagca tggcagctaa aaagaaagat aagatgtcaa catcaaagga caagtccatg
    14821 atggcagata aatcatctga taagaaaatg accaatgatg gtcctatggc gcctgatttc
    14881 gaactcaaag gcatcgatgg taaaacctat cgcttatcag aattcaaagg taaaaaagtt
    14941 tatttgaaat tttgggcctc ttggtgttca atctgtttat caaccttggc agataccgaa
    15001 gacctggcta aaatgtcaga taaagactat gtggtcttaa ccgtcgtctc gccaggtcat
    15061 caaggagaaa aatcagaagc agactttaaa aaatggttcc aaggaacaga ctataaggac
    15121 ttaccagtct tgttagatcc ggatggcaag ctattggaag cttacggcgt cagatcttac
    15181 ccaacagaag ttttcattgg aagtgatggt gttcttgcta aaaaacacat tggttatgcc
    15241 aaaaaatcag acatcaaaaa gacccttaaa ggtatacatt aggaaggacc agctgatagg
    15301 cattagaaaa aaggagttga gtttatggaa cacaaatgga aattatggct atttatcgtt
    15361 ggagtggtct ttttaatagg cggagcttct gttctcattc agaaaggtca agccaaggct
    15421 tttagtcaag gcaagcctca actaccagct aaaaaagtca aatccactaa agtagatccc
    15481 aatgcccctg ttgatcaaaa agtgatttac ctggctggag gctgtttctg gggcgttgaa
    15541 gaatattttt cacaagtgga tggcgttcta gatgcggtgt caggctatgc caatggtaga
    15601 ggcgatacca ccaactacca gttaattcat cagactggtc atgcagaaac cgttgaagtg
    15661 acctatgatg cgaaccgtat tagcttgaaa gaattattac tgcatttttt ccgaattatt
    15721 gacccaacca gtcttaataa gcaaggaaat gatcgcggta gtcaataccg aactgggatt
    15781 tactatactg acaaagcaga tttagctatt attgatgagg tcttcaaaga aaaagccaag
    15841 gattacaaga aaaaaatagt ggttgaaaaa gcaccattga aacactttat taaggcagaa
    15901 gactaccacc aagattacct caagaaaaat ccaaatggct actgccatat tgacatcaac
    15961 caagcgactt atcctgtgat tgatgaaagc aagtacccaa aaccaagtgc agctgagatc
    16021 aaagcaaaac tgtctgcaga cgagtaccga gtgacgcaaa aaaatgaaac tgaaaaagct
    16081 ttttctaatc gctactggga ttcttttgat gcaggaattt acgtagacgt tgtgacagga
    16141 gaaccacttt tctcttcaaa agacaaattt gaatcaggtt gtggttggcc aagttttagc
    16201 cgccccatca gcccagatgt tattcgttac aaggaagata agagtttcaa tatgacgaga
    16261 acagaggtgc gcagccgttc aggggattct catctggggc atgtctttac tgacggacct
    16321 aaagaccaag gcggccttcg ttactgcatc aatagcttgt ctattacctt tattccaaaa
    16381 gctgatatgg aagctaaggg ctatggttat ttattatcat ctgtagaata gggttaggcc
    16441 taaaaatctg atataataga agaaagacta gtatgagagg gccatgcaag gccctttata
    16501 aagataagga gacaccgtgt gtactcattg ttaattgtag aagacgaata ccttgtgcgc
    16561 cagggtattc gttctttggt tgattttagc cagttcaaga ttgatcgggt caacgaagca
    16621 gaaaatggcc agttggcttg ggacttgttt cagaaagagc cttatgatat tgttttgacg
    16681 gatatcaata tgcccaaatt aaatgggatt caactagcag aactcattaa acaggaatcc
    16741 ccccaaactc atctggtatt tttgacgggc tacgatgatt ttaactatgc cttatctgct
    16801 ttgaaattag gggcagatga ttacttgctc aaaccctttt ccaaggcaga tgtagaagac
    16861 atgttaggaa agctccggaa aaaattagaa ctttccaaga aaacagaaac cattcaggaa
    16921 ttggttgagc agcctcaaaa agaagtatca gcaatagcaa tggctattca tgagcggttg
    16981 gcagattctg atttgaccct aaaaagcttg gctcagcagc ttggttttag ccctaattac
    17041 cttagcgtct tgattaaaaa agagttaggg atgccctttc aagattatct gatacaagaa
    17101 cggttgaaaa aagccaagct tctcttgtta accagtaatc ttaaaatcta tgaaattgca
    17161 gagcaggtcg gttttgagga tatgaattat ttttctcagc gctttaagca actgataggc
    17221 gttaccccaa gtcagtataa aaaaggagac caggcatgaa gcgttatccc ctactggtcc
    17281 agttgatttc ttatatcttt gtgatcgtaa ttgccctcat taccacgctt ggtttgcttt
    17341 attaccagac gagttctcgt aatatcaggc aattaattga acgtgatact cggcagagca
    17401 ttcggcaaag ttcccaattt attgacgctt acatcaagcc tcttaaagaa acaacttcgg
    17461 tgctggcgaa aaatacggaa attcaagcct ttgctagtca aattcatcaa gaaaatgaca
    17521 agcaggtcct tcagctcatg aagatggttc ttgcgaccaa ttccgattta caagcagctg
    17581 ttctggtgac taaggatggt cgaacggtgt ctaccaattc tcagttgatt atgaaaacct
    17641 ccagtgatat gatggcagaa ccctggtata aagcagccat agaccgtcaa gccatgccaa
    17701 tcttaacccc agctcggcaa ttatcccttt cttctaaaaa agaatgggta gtttctgtga
    17761 cccaagaggt agtggatgct gctggtcata atttaggcgt gctaagactt gatattgcat
    17821 acccgaccat taaagcgtct ttagatcagc ttcagctagg ccgccaaggc tttgcattta
    17881 ttgtgaatga taagcatgaa tttgtttacc atcccaaaaa gagtgtttac agttcttcta
    17941 aggagatggc tgcgatgaag ccttatttgg ctattaaaaa tggctacgcc aaggataaat
    18001 catcctttgt ttatcaaagg gtcattccca acagtcaatg gactttgatt ggtgttgctt
    18061 ccttggacca attgcaccga gtacaacgtc agattttttg gtccttcctt gggatgggac
    18121 ttctagcctg tcttatttgt ggccttacga ctattttagt cttacgtaaa tggattcgtc
    18181 ccattcaaca attgcagcaa gttattcttg ctattcaaaa aggagatcgt cagttacgtg
    18241 cccaagaaac gggttctcca gaattgacag accttgccca acagtttaat gcgctcttag
    18301 atcaaattga tagcttgatg gttgccgttg cggataagga aaaggcgatt gggcagtata
    18361 ggttacaagc cttggctagt cagattaacc cgcattttct ctataacacc ttggacacta
    18421 ttatttggat ggcagaattt aatgacagca agcgcgtggt agaagtgacc aagtctctag
    18481 ctaagtattt tcgtttggcc cttaatcagg ggaacgaata cattcgtttg gcagatgaac
    18541 tggatcacgt tagccaatac ctctttattc aaaaacagcg ctatggagac aagctaagtt
    18601 atgacgttca aggcttagat gtctacgcag actttgttat tcctaagctt atcttacagc
    18661 ccttagtaga aaatgctatc taccatggca tcaaagaagt cgatcgcaaa ggcatgatca
    18721 aggttactgt atctgataca gctcagcatc tgatgttgac tgtttgggat aatggtaaag
    18781 gcattgaaga ctcttcactg accaatagtc agagcttgtt ggctagggga ggtgtgggcc
    18841 ttaaaaatgt tgaccagcgg ttaaaacttc actatggtga aggctaccac atgaccattc
    18901 atagccagtc agaccacttc actgaaatac aattaaccct tcctaaaatg catgagttaa
    18961 tggcagacga cacacaggaa aatgagtaag gttacaggaa gtgcatgtca gccatttccc
    19021 taagttaggg tattggagat aagaacctta gaaaggatat catgagttta gcatctgact
    19081 attttagccg acagaccccc atagttgaaa agctagtggc ttacggtttt gagaagcgag
    19141 acaacggtta tttctataat gaacggttta tggaaggaga atttgaggcg cagcttagga
    19201 ttgatgaggc tggcaatatc tgggatcggg ttattgattg tgatttggag gaggattatt
    19261 tgcctttaca acaagcagcc tggcaaggaa cctatactgg gcaggttaga gcagcctatt
    19321 tggaactctt ggggcgctta tcagtagcct gttttgaagt cactcctttt cagtcaatgc
    19381 aagctaatag acttgctaag catatcacca aagaatggtc tgacccaatg gactatcctt
    19441 ttgaaaagca cccagacctt gcaacctatc gggtaggggg caaatggtat gctatgattt
    19501 tttctctctt ggctgataaa ttagaccaga taccagagag attagtagga cagacatgtg
    19561 aagtgatgac tgtcaaagtt aatcccaagg ctttccctca gttactgcag caagaaggca
    19621 tttacccagc gtatcacatg tcaaagaaaa actggattag tattattttg gatgataagg
    19681 taacagatga taagctttgg acgttagtga ctcaaagtcg tcaactagtc aacccaaacg
    19741 gtctttctaa tcctaatggt cctgattatt gggttattcc agctaatttg aaatactatg
    19801 atattgatgc agaatttgca gccaatgatg tgattttatg gacccaaaaa gctggtattg
    19861 ccgtgggaga ctatgtgctg atttatataa cagccccagt caaatccatt cgctatgtct
    19921 gccaagttct agaaaccaat atacctaacg aaggctatcg taaaaatcct aacattgaca
    19981 aactcatgcg tctccgaaaa cgccaacagt acaaggatgg tctcttgtct tttgacctta
    20041 tgaaagaaca tggtgtagct gcagtgagag ggccaagacg attaagccct caattaattg
    20101 catttttaaa ggaaaaagag tattttaaag agaacaacta aaggagaata agagatggtt
    20161 gaatttgtag cagatcaaag tttttgggaa ctttttccag atgccaaact aggtgtcgtc
    20221 ttattaaagg atgttcagaa tcaagcagag tcaccagaag atgtcaaaca gctcttagca
    20281 gatagccatg agttagcaaa agcttattta acagcagaca attttagtga taatcaggtg
    20341 gttcaagtct accgcaaggc ctatcaacac tttaaaacca aaaaaggagc tcgctctagc
    20401 attgaagccc ttctcaagcg ggtgtcaaat ggtcaaagca ttccgtctat taacccacta
    20461 gtggatattt ataatgcggc tagtctacgt tttggtttac ctgttggagc cgaagatagt
    20521 gacagtttta tcggagattt gcgtctgacg ataacagatg gtggtgacga cttttacttg
    20581 attggggatg ctgacaataa cccaaccttg ccaaatgaac tgtgttataa agatgatatt
    20641 ggagcggttt gtcgttgcct taactggcga gatggcgaac gcaccatggt gacagaacac
    20701 accaaaaatg catttctgat tatcgaagct cttgatcaag aaggtcaaaa tagattgcaa
    20761 gaagctcttg acttcattga aggcagcgca aaaatgtacc ttcatgcaat cacttctgtg
    20821 catgtgttgg ataaagacaa tccacacgtg ctacttttta cagaaaacta gattagcagg
    20881 atgagctgtg taaaatagaa ggttaacaaa aaagcgagta aacgaatgtc ctgttcttta
    20941 ggttttcgtt tactcgcttt ttgatgagat cttactgctg ccctagcaaa aggtgaggat
    21001 gaggtcgtat atcacataga ggttttctgt taagccttat ttgttactaa agaagaatgg
    21061 tggtgtgtaa tgactaaggg tctcgtaaca agccttggct tgctcattgt cttcacagac
    21121 aatcaagcaa gtatcatcac cacagatcgt tgctacaatt tctggaattt gcatggcatc
    21181 taaaatggag ccaaaggatt gggctagtcc aggtaacgtt tttaaaataa tttgatgttg
    21241 aactatcttt aacatgacca aagcgtcttc catgtaaaaa cgaagacgat gttcccagcg
    21301 ggtttgtgaa atggctaacg cttcatagtg tgtgtcgttt ccagaagtta ctttgaccag
    21361 gttcaattcc ttcatgtcac gtgatagggt agcctgagtg atcgtgatgc catttttttg
    21421 caaccgttct tgtaattctt gctgtgtatg tatagttgtc tcagagatga gggaacgtat
    21481 gagttggtga cgcgtctctt tcttattcat tgttataatc ttcctttaac gtaattgata
    21541 acaatccgag tatagcaaaa agaggagaag gtgtaaagtt tttgatgaac cgcttacaaa
    21601 atggcttttt tatggtaaaa taaagatgat gtttagaaaa aggagatttg tgtgattcga
    21661 agagaagatt atcaatacct tagaaaacta aacgattttc gctacttttc tattgaacaa
    21721 tttgacaaaa ttgtagggca gatggaattc cgaaaagcta aaaaggacca tatccttttt
    21781 tttgagggag ataaaagaga taagcttttt ctagtgacct ctggttattt caaggtggag
    21841 cagtccgatc aatcaggaac cttcatgtat acggatttca tcagacacgg caccattttt
    21901 ccttacggtg gcctctttac agatgattat taccattttt cagtggtagc catgacagac
    21961 gtgacttatt tttactttcc agtcgattta tttgaagatt attcgcttga aaatcgtcta
    22021 caaatgaaac acttatatag taagatgtct aagctgctcg agttacatga gttgagggtc
    22081 cgaaacttga ttacatcaag tgctagttct cgtgtgattc aatctctggc gattttgctg
    22141 gttgaaatgg gtaaagacag tgatacctta ccttttcaat taacaacaac agatattgct
    22201 caaataagtg ggacgacaag agaaacagtt agccatgtgc ttagagattt gaagaagcaa
    22261 gaattgatta ccataaaagg taagtatttg acttatctag ataaagact
//
LOCUS       DATURV010000025        20454 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DATURV010000025 DATURV010000000
VERSION     DATURV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20454
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.025"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            38..286
                     /locus_tag="VCF75_000919"
     CDS             38..286
                     /locus_tag="VCF75_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP2546495.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            323..934
                     /gene="amaP"
                     /locus_tag="VCF75_000920"
     CDS             323..934
                     /gene="amaP"
                     /locus_tag="VCF75_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEP2546496.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGLTEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            945..1133
                     /locus_tag="VCF75_000921"
     CDS             945..1133
                     /locus_tag="VCF75_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEP2546497.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1146..1685
                     /locus_tag="VCF75_000922"
     CDS             1146..1685
                     /locus_tag="VCF75_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP2546498.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1726..1926
                     /locus_tag="VCF75_000923"
     CDS             1726..1926
                     /locus_tag="VCF75_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP2546499.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            1937..2425
                     /locus_tag="VCF75_000924"
     CDS             1937..2425
                     /locus_tag="VCF75_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP2546500.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTNAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2588..3229
                     /locus_tag="VCF75_000925"
     CDS             2588..3229
                     /locus_tag="VCF75_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2546501.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(3372..3914)
                     /locus_tag="VCF75_000926"
     CDS             complement(3372..3914)
                     /locus_tag="VCF75_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP2546502.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKTVTLDN"
     gene            4032..4733
                     /locus_tag="VCF75_000927"
     CDS             4032..4733
                     /locus_tag="VCF75_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546503.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEIDKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHCTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4748..7384
                     /locus_tag="VCF75_000928"
     CDS             4748..7384
                     /locus_tag="VCF75_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEP2546504.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMGLGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEVSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSTTSLSPYFNLLDC
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNVNHIKSIQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            7476..8072
                     /locus_tag="VCF75_000929"
     CDS             7476..8072
                     /locus_tag="VCF75_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP2546505.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLVNL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            8083..9039
                     /locus_tag="VCF75_000930"
     CDS             8083..9039
                     /locus_tag="VCF75_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP2546506.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVSTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            9136..10476
                     /locus_tag="VCF75_000931"
     CDS             9136..10476
                     /locus_tag="VCF75_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEP2546507.1"
                     /translation="MNEIKCPHCHTLFTINKSEYSQLLEQVRGQAFDEELQKRLINEI
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFAKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALVKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            10592..11476
                     /gene="truB"
                     /locus_tag="VCF75_000932"
     CDS             10592..11476
                     /gene="truB"
                     /locus_tag="VCF75_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEP2546508.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISQFERTSPLNFT
                     EDDLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            11505..12437
                     /locus_tag="VCF75_000933"
     CDS             11505..12437
                     /locus_tag="VCF75_000933"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEP2546509.1"
                     /translation="MEIEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFDKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNRTDSDYLTRNFEGQVYTIEEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            12480..12884
                     /locus_tag="VCF75_000934"
     CDS             12480..12884
                     /locus_tag="VCF75_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEP2546510.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDIDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            12886..13164
                     /locus_tag="VCF75_000935"
     CDS             12886..13164
                     /locus_tag="VCF75_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEP2546511.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSIYQVVKKAKAIEKGFFSLGN"
     gene            13154..13942
                     /locus_tag="VCF75_000936"
     CDS             13154..13942
                     /locus_tag="VCF75_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEP2546512.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVILEDQ"
     gene            13945..15255
                     /locus_tag="VCF75_000937"
     CDS             13945..15255
                     /locus_tag="VCF75_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEP2546513.1"
                     /translation="MMSLPKEFINIYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKESRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKSDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
     gene            15394..16260
                     /locus_tag="VCF75_000938"
     CDS             15394..16260
                     /locus_tag="VCF75_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEP2546514.1"
                     /translation="MKMKKKFFLLSLLALSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            16271..17206
                     /gene="pstC"
                     /locus_tag="VCF75_000939"
     CDS             16271..17206
                     /gene="pstC"
                     /locus_tag="VCF75_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEP2546515.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAVIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            17196..18083
                     /gene="pstA"
                     /locus_tag="VCF75_000940"
     CDS             17196..18083
                     /gene="pstA"
                     /locus_tag="VCF75_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEP2546516.1"
                     /translation="MNAKKVDKVATGTLYIIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSTMTAAK"
     gene            18099..18902
                     /gene="pstB"
                     /locus_tag="VCF75_000941"
     CDS             18099..18902
                     /gene="pstB"
                     /locus_tag="VCF75_000941"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP2546517.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            18915..19673
                     /gene="pstB"
                     /locus_tag="VCF75_000942"
     CDS             18915..19673
                     /gene="pstB"
                     /locus_tag="VCF75_000942"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP2546518.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            19741..20394
                     /gene="phoU"
                     /locus_tag="VCF75_000943"
     CDS             19741..20394
                     /gene="phoU"
                     /locus_tag="VCF75_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEP2546519.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
BASE COUNT         6460 a         3464 c         4248 g         6282 t
ORIGIN      
        1 acaaaaggta caggagtatc taaaaaggag aattatcatg ggacttattt ggactttaat
       61 cgtcggtgcg cttattggtg tgattgctgg agcacttact aaaaaaggtg gttcaatggg
      121 ctggattgca aacatcgctg ctggtctagt tggttcttcc gttggtcaag ctttacttgg
      181 ttcatggggc ccttctttag caggaatgtc tttgatcccg tcagttattg gtgccgttat
      241 cgttgttatg atcacttctt ttgtactaaa caaaactaac aactagtttc ctctttattt
      301 agtaagtact tacaaataga gaatgttaag tagaaaagag gtctgtatgt caaaattgtt
      361 aaagattagt tattgtcttg tcggacttgt tctattgtct gtttttggat gggtagttgg
      421 aataaccgga ggctatatat atttacctta tagttatcgt tggctaagtt ggggtatgga
      481 tagttttccc aatctcttag attcagcttt atcctattat tacttctgga cagcccttgt
      541 attatttgtt ataacatttc ttgccttatt agttattatt ttatacccaa gaatttatac
      601 tgaggtccag ttacgccata aaaataaaaa aggaacactt ttattaaaaa aatcggctat
      661 tgaaagttat gttgcgaccg ctattcaaac tgcaggtttg atgccaaacc caacagtcac
      721 agctaaatta tataagcgga agtttaagat tatcgttaaa ggacgtttag cttctcgtgt
      781 agcagtggca gatcaaatta gtggcgtgaa agaagggatc gaaaaaggtt taactgagtt
      841 cttcggaatt aattatcctg ttaatttcaa agtttatgtt aaagatattg ctgattctga
      901 tcgtaaacat attaccagaa atcgtgtaga ataggagatc tatcatggaa ttttatgaaa
      961 aattcaagta ccctattatt ggtggcttgg tggggctaat tattgccata ttgctcatgg
     1021 cttttggttt attcaaaacg ctactagcaa ttattttcat tattttagga atatatggtg
     1081 gcctgtatgc caaaaaaaca ggtattattg atcaatttct taatcgcaaa taaaggagac
     1141 ttattatgac tgaaacttat attaaaaaca catctaaaga cttaacttca gcaattcgtg
     1201 gtcaattaac atatgatgat aaagtaattg aaaaaattgt tggtttagca cttgaaaatg
     1261 tcgatgggtt actgggtgta aatggtggtt tttttgctaa cctaaaagat aaattagtaa
     1321 acaccgaatc tgtacgtgat ggcgtgaacg tggaagttgg aaaaaaacaa gttgccgttg
     1381 atcttgatat tgtcgctgaa tatcaaaagc atgtgccgac tatttatgac tctattaaat
     1441 cgatcgttga agaagaagtg aagagaatga ccgatctgga tgtgattgaa gttaatgtta
     1501 aagtagtcga cattaaaaca aaagaacaat ttgaagctga aaaagtaagt ttacaagaca
     1561 aagtttcaga catggcacgt tcaacatctg aatttacgag tcatcaagtt gagaatgtta
     1621 aagcttcagt ggataacggt gttgaaaaac ttcaagatca aaaagctgag ccacgtgtaa
     1681 aataattata tgtcgtcaat taggtaagct aaaggagggt ttattgtgtc agatgaaaaa
     1741 tataatgcga aattagatca agctggtggc aagttaaaag aaggatttgg taagatttct
     1801 ggagataaat ctttagaaac tgaaggtaaa gtagataaag ttactggaaa agttaaagag
     1861 gttattgcag acgccaaaga tactgtaaaa ggattagcta aaggcttaga taataaagat
     1921 aaataaaggg gacatgatgg aaaaaactca aattaaaagc actcttactt atgacgataa
     1981 ggtcatcgaa aaaattgtcg gtcatgcttt ggaaaacgtt ggcggattac tagctgtaac
     2041 aggtggattt ttctcaaata ttaaaaataa tttggtgaat tctgagtctg taactgatgg
     2101 tgtttcagtt gaagttggta gcaaggaagt agcagttgat ttagcaatca ttgtagaata
     2161 cggtaaagat attcctgcta ttgcagaatc tattaaagct attgtttctc aaaacgttga
     2221 tagtatgaca catttgaaag ttgttgaagt caatgtgaac gttgttgata ttcgtactaa
     2281 ggaagaacat gaagctgcta gcgttactgt tcaagatcgt gtcactaatg cagcttcaag
     2341 tacttcacaa tttgtatcag aacaaacgga aaaattaaaa gatactatct ctgatactgt
     2401 aaatagtgat gaggcagcta aataagcaat cgtctaggaa gtattattga ctagtggtga
     2461 taagtttgag ctggtattta ccagctcttt ttgtcacggt taaaaagaca cggactattg
     2521 tcatctctct aaaaatatga tttaattaga tagaaatttt tcaaattgtt agaaaggagt
     2581 aaattaagtg ataagtccta aaaaagagat aacaagttca aaatatcaaa aaattgctat
     2641 ttcagtagct cagcgtatcg caaatggcga atatgaagtg ggcgaaaagt taaagtcaag
     2701 aactactatc gcctcgacat ttaatgtatc accagaaacg gctcgtaaag gcctgaatat
     2761 tttagctgac ttgaaaattt tgactttaaa acatggtagt ggagctattg tgctgtcaaa
     2821 agaaagagct attgagttta taaatcaata cgaatcaacc cattccatcg ctgttttaaa
     2881 agaaaaaata cgtgagacta ttaatgatca gggaaaagca atggaaaaaa tggctgtttt
     2941 ggtcaatgat tttttaatgc aaagtcaatc tgttagtaag caatacccat tagcacctta
     3001 tgagattatt tgtagtcaag attcggaaca ttttggcaaa tctattggtg tgttaaatat
     3061 ctggcaccag acaggagcaa ctatcgtcgc tattgaacat gctggtcagt ttatcgtttc
     3121 accaggtcct tattctgtta ttgaaaaagg cgatcatatt tatttcgttg gtgatgagtc
     3181 agtcatttca cgaatgaaga ccttttttaa tttacgaaaa ggattataag tttgagggcc
     3241 cacctcaaac tttttttgtt gctgttgagc attattaaaa gaggcagatc atcatggttt
     3301 gtctttccaa aatatctgtt tagacagttg ttttggattg ggagcatgat ttcagactct
     3361 tttgatgttt tttaattgtc tagagtaact gttttgagaa taatatcggc aacttcttca
     3421 gtggtttctt gatagccatt agcaatccaa tagctgataa tgctctcaat actggcaagg
     3481 taaacttcta aagcataggg ataaggaata tggtattgat tggtcacaat gtcttggaaa
     3541 ccagtaatag tagtcaaaaa ttggtaacag aaatccttga tgagttgcgg aaatttgagg
     3601 taagaaatag tagcgagtgc ggttataaat tctctatgtt ctataaggta ggacaaggtt
     3661 tgattgagga cttgtcgagt gtctgtgtag atttcagcct ggtttaacaa acggtacaaa
     3721 tcatccagag tgtcattttt gaagtgattc atcatgtcaa atttatcagt gtagtggagg
     3781 tagaaggttc ctctattgat accagccttt ttagtaaggt cagagacagt aagagtttca
     3841 aaagattgtt cagttagtaa ggtggttaag gcggtcttta cataagcttt ggtttcagta
     3901 tgtcttttgg tcattctcag gtgctcctta aatcaacagt tgcgtcagat ttgttcttgt
     3961 taaatactta gggccattat ataataaggc tataattaaa tcaacactat gttcaaataa
     4021 tggaggggca gatggctttt attgaattaa aacaggtttc aaaatcttat caggtaggag
     4081 agacgactgt ttttgccaac cacgaagtgt cttttgagat tgataaagga gagctggtgg
     4141 ttattttggg agcatccgga gctggtaaat cgacagtcct caatattttg gggggcatgg
     4201 acacagtaga tgcaggtcaa gtaattattg atggcaaaga cattgctcat tgcacatcta
     4261 aggccttaac tcagtatcgt cggaatgcta ttggtttcgt ttttcaattt tataatttgg
     4321 ttcctaattt gacagctaaa gaaaatgttg aattagcagt tgaaattgta gcagatgctt
     4381 tagatcctgt gaccatttta aaggaagtag gactcagtca tcgtctggat cattttcctg
     4441 ctcagctctc aggtggtgaa cagcaacggg tttcgatagc acgtgcctta gctaaaaacc
     4501 ctaaattgct tctttgtgat gaacctacag gtgcccttga ctaccaaaca ggaaagcaaa
     4561 ttctaaccct cttacaggat atggcacgaa ctaaggggac cacggtagtt attgtaaccc
     4621 acaatgctgc tattgctccg atagctgatc gtgtcatttt tatgcatgat gcacaggtta
     4681 ccaagacagt cattaataag gaacctgcaa gcattgagac aattgattat taagaggaag
     4741 gtgtcgtatg ataaagaaaa ccttatggaa ggatattttg agagctatca agaatagtaa
     4801 agggcgattc atttccttat ttttcctaat gggcttaggc tcctttgctt tagttggact
     4861 taaagtcacc ggtccagata tggaacgaac agccagccgt tacctcgaaa gacaccaggt
     4921 gatggaccta acggtactgg cttctcatca attttcccaa gccgataaac aagagttaga
     4981 tacgttaaaa ggggctcatt tagaatatgg tcatttactt gatgtcagtc taactagcaa
     5041 ccagaaatct cttaggctct atagcgtgcc aaagaaagtg tctaagccag tcttggttaa
     5101 agggagttgg ccaaagcgag agacagattt ggttttatcc tcctcacttg ctaaaaacta
     5161 tcagattgga gatgaactag cagtcacctc acctatggaa ggtttgctga cgacaaccca
     5221 ttttcaagtg gttggttttg cgaattcttc agaagtttgg tccaagtcca atttaggaag
     5281 ttcctcaacc ggagatggaa gtctctacgc ttatgctttt gttaatccaa acgtttttaa
     5341 aagtgccttt aacctgctgc gcatccgttt tagtaatctt cgtctgacaa acgctttttc
     5401 caaagactat caaaaaaggg taacccaaaa ccaagctcat ttggataatc tacttaaaga
     5461 taatggccag aagcgttatg atgacctcca aaatcagtat gaccttgcct taaaaaatgg
     5521 cagagcagca cttgcaaagg aaacagtgaa actagctgcg agtgaggaga acttaacttt
     5581 tttagaagtc tctgctttac aagaagctaa gcatcagatt gaacaaggca aacaagcatt
     5641 agccaaggag gaaaagcagt tagagcaggt gcaggctaca aaagataagc tagaaaaacc
     5701 cagttacctg acttataatc gctcgaccct accaggagga gaaggatatc atacttatgc
     5761 aacttcaacg acctccattt caaatgttgg aaatattttt cctgttgttc tttatctcgt
     5821 agctgctcta gtagccttta ccaccatgac acgttatgtt gatgaagaaa gaacaagctc
     5881 tggtctatta aaagccattg gttattctaa caaggatatc agtttaaagt ttcttatcta
     5941 tgggctttta gccagttttt tagggacaac tttaggtatt attgggggaa cttacctctt
     6001 atctgccttg atttcagaga tcttaacagg agctttgact attggaaaga ctcaccttta
     6061 tagttattgg ttttataatg gcatagctta cttgctggct atgttatctg ctgttttacc
     6121 agcctactta attgtcaaaa aggaattatt cctcaatgca gctcagttat tgctgcccaa
     6181 acctcctagt aagggggcaa aaatctggtt ggaacacctt acttttgtct ggaaagctct
     6241 gtcctttact cacaaggtga ccatacgtaa tatttttcgc tataaacaaa gaatgctgat
     6301 gaccattgta ggcgttgcag gctctgtagc ccttttattt gcaggcttag ggattcagtc
     6361 gtcattagcc aaagtagttg agcatcaatt tggtgattta acgacttatg atattttggc
     6421 tgtcggttcg gccaaagcga cagcgacaga gcaaactgac ttagctagct atcttaaaca
     6481 agaacctatt acagggtacc aaaaggtatc ttatgccagc ttaacccttc ctgtaaaggg
     6541 attacctgat aagcaaagta tttccatttt atcaagttca actacctctc ttagccctta
     6601 ttttaatctg ctggattgtc aggagcaaaa gaaggttccc attccaacct ctggtgtttt
     6661 gatttctgag aaattagcct cttattacaa ggtaaaacca ggtgatcagt tggtattgac
     6721 tgatcggaaa ggacagtctt ataaagtgac gattaaacag gttattgaca tgacagttgg
     6781 ccattacctg ataatgtcta atacctattt taagaatcat tttaaaggat tggaggctgc
     6841 tcctgcctat ctgattaagg taaaagacaa agatagcaag cacataaagg agacagccag
     6901 tgacttgtta accttaaaag cgattagagc agtttcacaa aacgtcaatc atattaaatc
     6961 tattcagcta gtagtcacct ctcttaatca ggtcatgacc ctccttgtct tcttgtctat
     7021 tttattagca atcgttatcc tttataactt aacgactatt aatattgctg agcgtattcg
     7081 agaattatcc actattaaag ttctgggatt ttacgatcag gaggtcacct tatatattta
     7141 tcgagaaact atttcgctat ccctagtagg cattctttta ggtatctatt taggaaaagg
     7201 cctgcatact tatatcatga caatgatttc aactggggat attcaatttg gtgtaaaggt
     7261 tgatgcttat gtttatctag tgccaattct agtaatcctt agcttgttag cggtattagg
     7321 tatctgggtt aatcgccatt taaaaaaggt tgatatgtta gaagctttga aatccataga
     7381 ttgattggtg aaaagacaga tatagacagc acttgaaaag ttaatcatat tatgatagta
     7441 tctaaatact ataatttaat agaaagatac tatgtatgga aaatagcatt gaggcctatc
     7501 tagcagatag cagagaaaag gtaacattag gtacagttgt tgattgtttt aaaggaaaag
     7561 cagtctcaag taaggttgtg ccaggtgatg tgggattggt taatttatct gacatgggca
     7621 ccttagggat tcaatatcac caattaagaa ctttccagat ggatcgtagg cagctcttgc
     7681 gttacctttt ggaagacgga gatgtcttaa tcgcctctaa gggaacccta aaaaaagtct
     7741 gtgtttttca taagcaaaat cgagatgtgg tggcatcatc aaatatcact gtcttacgcc
     7801 ctcaaaaact gttaaggggc tactatatca agttcttttt ggactcacct attggtcaag
     7861 cgctcttgga cgcagcggac catggcaaag atgtgatcaa tctctcaacg aaagaattgt
     7921 tggatattcc aattcccgtt attcctttag tgaaacagga ctatttgatt aatcactact
     7981 tgagaggatt aacagattat catcgtaaac tcaatcgagc agaacaggag tgggaatata
     8041 ttcagaatga aattcaaaaa ggattaggtt aggagtcttg tcatgggttc agttgaacgt
     8101 acattaaaaa tgaccttggc taccattgtt gccattttaa ttgcttacca acttcatctg
     8161 gattatgcaa tgtcagcggg gatcattgcc ctgttaagtg ttttagacac ccgtaagtcc
     8221 agtttggtgg tcgctaggaa tcgcttgcta tcttttttcc tggctttcgg gattgcgatg
     8281 atgtgcttta gcttgtttgg ttttacgaca gtaggtttta tgtgttattt gttaattatt
     8341 attcctttgc tctatcactt tcagatagag gcaggactgg ttcctataac agtacttgtg
     8401 acacatttga ttgctaaaaa gagtattgca ttacctattt tatcgaatga gtttatgctc
     8461 ttttttgtag ggactagtgt cgctttacta tttaatgctt atatgggtcc acaagatcag
     8521 caaattcggt actatcacca aaaagttgaa tcggatctga aaggtatctt ataccgtttt
     8581 gaaagttttc tactggaagg aaaaggtcaa aatgaagggc tcttgatcaa aaaccttgat
     8641 aaaatattag atgaagctct aaaactagtt tatcgagaaa ggcataatca actatttcag
     8701 cagaccaatt atcaagttca ttattttgag atgcgacgac aacaaaatag actattggga
     8761 caaatggcta tcaacgtaag cacgttaatg agacaaagta aggaaagtat tcttttgtca
     8821 catctttttc acgaaactgc ttgtcagcta agcgaacaaa atccggcttt aaccttgatt
     8881 gatgacatcg aacaattgct tgaaaccttt cgtcatggtg atcttcctca aactcgggag
     8941 gaatttgagc gacgtgcggt tttatttcag ctcttacaag acttggaacg ttttatctta
     9001 ttaaaggtag agttttatca ggattatcaa aatgactaaa tctctattgc acacttctta
     9061 tctatgacag cttgggactt aacttcctga gcttttttgc tataataaaa acaagaatct
     9121 cagaatggag acaagatgaa cgaaataaaa tgccctcatt gtcataccct atttactatc
     9181 aataagtcag agtatagtca attattggaa caagtcagag ggcaagcctt tgatgaagaa
     9241 ttgcaaaaac ggctaatcaa tgagatagcc ttgctagaag aaaaagcgaa gcatcaattg
     9301 catgaggttg tcgcaaaaaa agaaacagct attaccagct tgacaaacca attagagcaa
     9361 attgaaaaag aacaagccta cctcagacaa gaagaactgg ctaaaaaaga ccagcttatt
     9421 gcttctttag aagcgaaact tgataaatta gcctcgcaaa atgctttgga actggccaat
     9481 caattagctg agaaggacaa agaagtggtt agtttgacca atcagctgga caaattagca
     9541 cttgaaaaag atgccacgtt ccagtcaaag cttgctacaa tcgaaaaaga acgcgatgga
     9601 atcaaaaacc aattggcgct tcaagctaaa gaaagtgaat tgtcgttggc ttcggtgcgc
     9661 agcgactacg aagcacagtt aaaagcggct aacgagcaag ttgaatttta taaaaatttc
     9721 aaagcgcagc aatctaccaa agcaatcgga gagagtctag aactctatgc tgaaacagag
     9781 tttaacaaag taagaagcta tgcttttccg aatgctagct ttgccaaaga taaccaattg
     9841 tctagtcgtg ggtcaaaagg ggactatatt taccgagagg ttgatgccaa tggtgtagaa
     9901 atcctctcta tcatgtttga gatgaagaac gaggcagaca ccaccaaaac aaaacataaa
     9961 aatagtgatt ttttcaagga gttagataaa gaccgtcgcg aaaaagattg tgagtatgct
    10021 gttttggtaa gcatgttaga agctgataat gattactaca atacagggat tgttgatgtc
    10081 agtcatgagt atcaaaagat gtatgtggtt cgtcctcaac tctttattca gttgataggg
    10141 attctgcgaa atgcggccct gaatagttta cattataagc aagagttggc cttggttaag
    10201 gaacaaaata ttgacatcac tcacttcgaa gaggatttgg accaatttaa gaatgccttt
    10261 gctaagaact atcagtctgc cagcaacaac tttaaaaagg ctattgacga gattgataaa
    10321 tcgattaaac gcatggagga agtgaaacgc tttttaacca ctagtgaaaa tcagttacgc
    10381 ttagctaata ataaattaga ggatgtttca gttaaaaaac tgacccgcca aaacccaacc
    10441 atgcgagaga aatttgaagc cctcaaggac cagtaagcct gaaagtgagt ctttccttgc
    10501 taaaattggt taagcgttta ttttggcaaa actgatgata aagcggtaga atagaatcag
    10561 taacaattac taattagaga gaaaaataga catgataaat ggaatcatta atttaaaaaa
    10621 agaagccggt atgacctctc atgacgcagt ttttaaatta cggaagttat tacaagaaaa
    10681 aaagattgga catgggggga ccttagatcc tgatgtggtc ggtgttctac caattgcagt
    10741 tggtaaggct actcgtgtga tcgaatacat gacagaagct ggcaaagttt atgaaggaca
    10801 agtgactctc ggttactcaa caacgactga ggatgctagt ggagaggtag ttgcgaggtc
    10861 ctctttgcca gctgtgctaa cagaagaact cgttgatcaa acgatgacga ctttccttgg
    10921 gaaaatcaca cagaccccac ctatgtattc agccgtaaag gttaatggcc gtaaattata
    10981 cgagtatgcg cgtgctggtg aaagtgtgga acgtcctaga agagaagtga ccatttccca
    11041 atttgaacgc accagccctt tgaattttac tgaagacgat ctctgccgat tctcttttaa
    11101 agttgcttgt agcaagggaa cttatgtgcg gaccttagca gttgatttag gtcgagctct
    11161 aggggtagag agtcatatgt ccttcttgca gcgatcagct tcagcaggat taaccctcga
    11221 gactgcctat actttgggag aaattgctga tatggtttct aaacaagaga tgtctttttt
    11281 gttaccgatc gagtatggcg ttgctgatct tcctaaaatg gttattgatg atactgaatt
    11341 gactgaaatt tcatttggtc gccgtcttag tctccctagt caagagccac tgttagctgc
    11401 ttttcatggc gaaaaagtaa ttgctatttt agaaaaaaga gatcaagaat acaaacccaa
    11461 aaaagttctt atttaaatag tttgagtaag tagaatggat aattatggaa attgaatata
    11521 tcaaagatta tcgtgatatt aaccaagaag atgatacagt cttggtattg ggatattttg
    11581 atggattaca ccgtggtcat aaagccttat ttgacaaagc aagagaggtt gcaaataaag
    11641 aagggttaaa agtagtcgtt tttacgttta cagagtctcc gaaattggct ttttcgcgtt
    11701 tttctcctga attattgctt catattactt atccaaagaa gcgttatgaa aaatttgccg
    11761 attacggtgt aaataaatta tatttggttg attttacttc taaattctct aaggtctcat
    11821 ctgatcattt tataactcat tatattaaaa acctgaaagc aaagcatatt gttgtcggtt
    11881 ttgactataa gtttgggcat aatcgtacag atagtgatta tttaaccaga aattttgaag
    11941 gacaagttta taccattgag gaaataaaag aagatcatcg aaaaatttct gctacatgga
    12001 taaggaagct tattcaagaa ggtaatgtag ttaaagcaaa ccacttgtta ggctatgact
    12061 tatcgacaag ggggagagtc gttcacgggg atgcgcgtgg ccgtactatt ggtttcccaa
    12121 cagccaatct agctccaatt gacaacacat acttaccagc tgatggagtc tatgttacaa
    12181 atgttattgt tgctaataaa atctatcgct ctatgacgag tttaggtaaa aatgtgacat
    12241 ttggggggaa agagttgcgt ctagaagtta acatttttga ctttgacgaa gagatctatg
    12301 gagaaattat tgagattgtt tggttagata agattcgaga catggaaaaa ttcgagggaa
    12361 tcgaagattt gactgataga ttggaatatg ataaacgaac agcattaaat tggaaaaaag
    12421 atagcaaatt atcttaaatt tttgtataat aaggtaagta aatttgatag aggaaaaata
    12481 tggttacctt atttttatca cctagctgta ccagttgtcg taaagcaagg gcatggttag
    12541 taaaacacga agttgacttt caagagcata atattattac aagtccatta agccgtgatg
    12601 aattaatgtc aatcctctct tttacagaga atggaacgga ggatattatt tcaacacgtt
    12661 caaaggtttt tcaaaaactt gatattgatg tagaagagct ttcaatttca gatttaattg
    12721 atttaattgc taaaaatcca agtcttcttc gtcgccctat tatcatggat caaaaacgca
    12781 tgcaaattgg ttttaatgaa gatgaaatta gagcgttttt atcgagagat tatcgcaagc
    12841 aagaactacg tcaagcaacg ataaaagcag aaattgaggg gtgatatgtc aggtaattat
    12901 tattatccac ttgacttaag ttggagcact gaagaaatat cctcagtgct tcattttttg
    12961 aataaagtgg aattagcata tgaaaaaaaa gtagatgcaa aacaactact agatagttat
    13021 aagacttata aaaccattgt caaaagtaaa gctcaggaaa agcaaatcga ccgtgatttt
    13081 caaaaagtta gtggttattc gatctatcaa gttgttaaaa aagcgaaggc aattgagaaa
    13141 ggattctttt cccttggaaa ctaaatacgc atttgcaagg caaatcatta aagaagcagg
    13201 gctttttatc aagtcaaaaa tgtcagagca gctagatatt caggtgaaaa cacaatttga
    13261 tgatctggtt accaatgttg atcaagaaac tcaacagtta ctgatggatc gtattcatca
    13321 gacctatcct tgtgatgcca ttttagctga agaaaatgac gttcgtcacc ccattaatca
    13381 aggaaatgtt tgggtcatcg atcccattga tggaacggtt aactttattg tgcagggatc
    13441 acagtttgca gtgatgattg cctattatga acaaggaatt ggccaatttg ggcttattta
    13501 tgatgtgatg gcagatcaat tactagctgg tggtggtgat tttgaagtta ctttaaacgg
    13561 tgataagctt ccagcttacc aagaaaaacc tttagagcgt agcttaattg gctgcaatgc
    13621 aggcatgttt gccagaaatg atcgtaacct tgctcatcta attgcaaaga ctttaggtgt
    13681 aagggtttat ggtggtgcag gtatctgcat ggttaaggtc atgaagcagg aattactggc
    13741 ctatttttca tttatccaac catgggatta cgcagcagca aaagtactag gtgataaact
    13801 gggttatgtt ttactaacta ttgacggata tgaacctgat tttcaaacac gtcaaaaaat
    13861 catgtttgtt cctaaatgcc aactcactag gatagcgtct tttctaacaa aggactctgc
    13921 cgtcatactt gaagatcaat agaaatgatg tcattaccaa aagaatttat taacatttat
    13981 caagcaatac taggcaaaga attagaagat ttcttggcaa gttttaacca agagccggtc
    14041 aatgcttttc ggattaaccc cttaaagaat caactaaaaa cctttgaaca ccccattcca
    14101 aataccttat ggggatatta tggtaaacta tctggcaagt ctccagagca tgtgtcgggg
    14161 ttggtttatt cccaagaacc agcggctcaa atggtggcac aagtagcggc tcctcaaaag
    14221 gagagtcgtg ttttagactt ggcagctgca ccaggaggaa aatcaactca tttattagct
    14281 tatttagaca atacaggtct tttagtcagc aatgaaattt ctaagaagcg cagcaaggtt
    14341 ttagttgaaa atatcgaacg ttttggcgct agaaatgttg tggtgactaa tgaatccgca
    14401 gatcgtttag ccaaggtatt ttctcattat tttgatacta tcgtctttga tggaccatgt
    14461 tctggtgaag gaatgtttcg caaggaccct gatgctattc aatattggca tcacgggtat
    14521 cccgcagaat gcgccaagtt gcaaaaatct attttagaag acgccttaac catgttaaaa
    14581 ccaggaggag aattgatata ctccacctgt acttgggctc ctgaagaaaa tgaagatgtt
    14641 gtgcagtggc tcttagagac ttataccttt ttagaattgg tcgatgttcc aaaactaaat
    14701 ggaatggtta gtggtatcgg acttccagag actgctagaa tgtaccctca tcgttatcaa
    14761 ggggaagggc aatttgtggc caagctaaaa gataagcgtc aagaaggaca aagcactaaa
    14821 ctaaaagcac ctaaatccaa cttgaccaaa gaccagttac gtctttggaa aatgtttgaa
    14881 aaagaccatc taaaccttac cctatcagga acactacaaa cctttggtga ttacctatac
    14941 ctcttgccag atggtcttcc tgctttagat ggccttaaaa ttgctcggaa cggtttagaa
    15001 ttaggcgtct tcaagaagaa acgctttgaa ccctcttacg ccttgggatt agctttaaag
    15061 tctgatgagg tccgatcatc tattgagctt agagaggaag actttagacc ttatgtgtca
    15121 ggtaacgtca ttactttgcc ccaatcttat ccaacaggct ggtaccaatt actcattaat
    15181 ggcaatggtc taggatttgc caaggtcgtt gacaagacca tcaaaaataa tttccctaaa
    15241 gggttaagat tttagcgtaa aaaataagat taaaatgtta aatatttaaa gaaaaaatgc
    15301 tttcaaagca taggtagata tgttactata aagaatatgt ggcacttagt ttctacatat
    15361 atacctttta tttttacgaa taggaaataa ataatgaaaa tgaaaaaaaa attctttttg
    15421 ttaagtcttt tagccctatc aactttcttt ttatccgcat gttctagctg gattgataaa
    15481 ggcgagtcaa taaccgctgt aggatcaaca gcactacaac ccttagtaga agcagtagct
    15541 gatgaatttg gaagcagtaa tctaggtaag actgtcaatg ttcaaggtgg tggttcaggt
    15601 acagggttgt ctcaagttca atcaggagct gtccaaattg gaaatagtga tgtctttgca
    15661 gaagaaaaag atggtattga tgcttctaaa ttagttgatc atcaagtagc tgttgcagga
    15721 cttgcagtta ttgccaatcc taaagtcaag gtttccaatc tcagtagtca gcagttgcaa
    15781 aagatttttt caggagaata taccaattgg aaacaagttg gaggagaaga tcttgcgatt
    15841 tcagtgatca accgagcagc aagttctggc tcacgagcaa cctttgacag tgttatcatg
    15901 aaaggggtca acgctaaaca aagtcaagag caagactcca atgggatggt taaatcgatt
    15961 gtttcacaaa caccaggtgc catttcttac ctttcctttg cctacgttga ttcatctgtt
    16021 aaatctttgc aattaaatgg gtttaaggca aatgctaaga atgtggctac aaatgattgg
    16081 ccaatctggt cctacgaaca catgtacacc aaagataaac caacagggtt gaccaaggaa
    16141 tttcttgatt atatgttttc agatgaagta caacagaaca ttgttacaca tatgggatat
    16201 atttcgataa atgatatgga agtggtcaaa tctcatgatg gaaaagtaac aaaaaggtaa
    16261 tggaggaatt atggaaaatc aggaattggc taaaaaatta gcctcaccat caaagaattc
    16321 aagacttgaa acctttggcc gcaccattac gtttttgtgt ctggctttga ttgtttttat
    16381 cgttgcgatg attctgatct tcgttgctca aaaagggttg tcaaccttct ttgttgacaa
    16441 ggttaatctt tttgatttct tgtttggaaa ggagtggcaa ccaagtgtga aaaatgcggc
    16501 cggcattcct tatcttggtg ctctgccaat gattacagga tcctttttgg ttaccatttt
    16561 atctgctatt attgccaccc catttgccat tggcgcagcc gtttttatga ctgaaatctc
    16621 acctaaatat ggcgctaaat tattacagcc tgcggttgag cttttggttg gaattccttc
    16681 ggttgtttat gggtttatcg gtttgcaagt gattgttcct tttatgcgct ctatctttgg
    16741 tggcacaggt tttggaatcc tatctggggt ctgtgtcttg tttgttatga ttttaccaac
    16801 agtgactttt atgacaacag acagtctgcg ggcggtgcct cgtcattacc gcgaagcgtc
    16861 tatggctatg ggagcaacac gttggcaaac catttggcgt gttgtcctta atgcagctcg
    16921 tccaggaatt tttaccgctg ttatttttgg aatggcgaga gcttttggag aagccttagc
    16981 cattcaaatg gttgtcggta actctgctgt aatgccaagt tcactaacga caccagccgc
    17041 aaccttaacg tctgtcttga caatgggtat tggtaacacg gttatgggaa cggtccaaaa
    17101 taatgtgctt tggtcccttg ccctagtctt attattaatg agtctggcct tcaattccct
    17161 tgtcaaatta atcacgaaag aaagaaagag aaattatgaa cgctaaaaaa gtcgataaag
    17221 tagcaactgg tactctttat atcattgctg gaattattgt agctatttta gcttccttaa
    17281 ttctatatat ccttgtccgt ggcttgccac acatcagctg gtccttttta acaggaaaat
    17341 cgtcttcgta cgaagctggt gggggaattg gtatccaatt gtataattcc ttcttcctat
    17401 tgattgttac cttgattatt tccattcctt tatcaactgg agcggggatt tacttggctg
    17461 aatatgccaa aaaagggcct gttaccaact ttattagaac ctgtattgag attctgtctt
    17521 ccctaccatc tgtggttgta gggctctttg gttacttgat tttcgttgtg cagtttgaat
    17581 atggcttttc tattatttca ggggctcttg ctttgacggt ctttaatctt cctcaaatga
    17641 cccgtaatgt tgaagatagt ttactgcatg ttcatcatac ccaaagagaa gcaggattag
    17701 ctttaggcct gtcacgctgg gaaacggttt tttatgtggt tatcccagaa gctctcccgg
    17761 gaatggtaac aggtattgtc ttagcttcag gtcgtatttt tggtgaagcg gcagcgctta
    17821 tctatactgc tggtcaatca gcaccagctc tggattggtc aaattggaat ccacttagtg
    17881 ttactagtcc aatctctatt ttccgtcaat ctgagaccct tgctgttcat atttggaaag
    17941 tcaatagcga agggacaatt ccagatgcta ctcttgtatc cgcaggaagt gcagcagtgc
    18001 tattaatttt tatccttatt ttcaactttt cagctcgctt tattgggaag aaacttcatt
    18061 ctacaatgac agcagcgaaa taagatattg gagaaatcat gacagaatac aattggaatg
    18121 aacgtcatat catcactttt cctgaagaaa cgcttgctct agctaccaag gatttacatg
    18181 tttactatgg tgcgaaagaa gctatcaaag ggattgatat gcagtttgaa aaacataaaa
    18241 taacagcctt gattggtcca tctggttgtg gcaaatcaac ttatcttcgt agtcttaacc
    18301 gcatgaacga taccattgat attgctcggg ttacaggtga gattctttat cagggcattg
    18361 atgttaatcg aaaagacatg aatgtttacg agattcgcaa gcacttagga atggtttttc
    18421 aaagacccaa tcctttcgct aaatccattt ataaaaacat tacctttgcc catgaaagag
    18481 ctggtgtgaa agacaaaaaa gttttagatg aaattgttga gacttcactg aaacaagccg
    18541 cgctttggga tcaagttaaa gatgaccttc acaaatcagc cttcacctta tcagggggtc
    18601 agcaacaacg cttgtgtatt gctagagcca tttctgtcaa acctgatatt ctattaatgg
    18661 atgaaccagc ctctgccctt gacccgattg caacgatgca gctggaagaa accatgtttg
    18721 aattgaagaa gaattacacg attattattg tgacccacaa tatgcaacaa gcagctcgtg
    18781 ccagtgatta cactgccttc ttctatttag gcaacctcat tgaatatgat aagacacgta
    18841 atattttcca aaatgcgcaa tgtcagtcaa caaacgacta cgtttcaggg cactttggtt
    18901 agaaaggtaa tatgatgaca gaacctattt tacagatcag agacctttct gtttactaca
    18961 atcaaaagaa aacgttaaaa gatgtctctt tggaccttta tccaaatgaa ataacagctt
    19021 taattggtcc ttctggatct gggaaatcaa cgctcttgcg ttctatcaac cgtatgaatg
    19081 atttgaatcc tgaagtaacc attacaggct ctatcgtcta taacggacac aatatttata
    19141 gtccaaggac agatacggtt gatctacgca aagaaattgg tatggtattc caacaaccca
    19201 acccatttcc gatgtctatt tatgagaacg tggtttacgg ccttcgttta aaaggtatca
    19261 gagataaatc aatcttagac catgctgttg aaagctctct taaaggggct tccatttgga
    19321 atgaagtcaa agatcgttta catgattctg cggttggctt atcaggcgga cagcaacaga
    19381 gagtttgcat agctagagtt cttgctacca gccccagaat aatcctttta gatgaaccta
    19441 cctcagctct tgatcctatc tcagctggga aaattgaaga aacattattg ttattaaaaa
    19501 aagattatac actggctatt gtgacccgct caatgcagca agcctcacgt ttatcagatc
    19561 gaacaggttt tttcttggag ggtgacttac ttgagtgtgg tcctacaaaa gccatgttta
    19621 tgaatcctaa gcgcaaagaa accgaagact atatttctgg taaatttggt tgagaaagac
    19681 gtatatggtt gacccctctg tgtttttatt ataatgacta gattgtagga agaaaaaatc
    19741 atgttaagaa cgaaatttga agaagaatta gataaattgc ataaccagtt ttattcaatg
    19801 ggaacggaag tattggcaca aatcaataaa actgttcgtg cctttgttag tcatgatcgt
    19861 gaattggcca aagaagttat cgaagaagat gacactatta ataattttga gacaaagctt
    19921 gaaaagaaat cgctggaaat tattgcccta caacagccag tttcgaatga tcttagaatg
    19981 gttattactg ttttaaaggc atcaagtgat attgaacgta tgggtgacca tgctgcttcg
    20041 attgccaaag ccactattcg tatgaaaggt gaggagcgta ttccagtggt tgaagaacaa
    20101 atcaacctta tgggaaaagc tgtgaaacag atggtagaag aagcccttaa tgcatatatc
    20161 aatgctgacg atacaaaagc ttatgaaatc gcagcttctg atgagattat cgatcaatat
    20221 ttccgtaata tccaaacctt agctgttgaa gaaatccgta agtctccaga tgctgttttt
    20281 gcaggaaaag aatattttca agtattaatg tatttggaac gtattggtga ctatgctcgc
    20341 aacatttgtg aatggattgt ttatcttaaa actggaaaaa ttattgaatt ataagaaatt
    20401 atgctataat gagactggga aacagcttgc cagtccctgg ttttatacga aatt
//
LOCUS       DATURV010000026        20398 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DATURV010000026 DATURV010000000
VERSION     DATURV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20398
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.026"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            32..3247
                     /gene="rexB"
                     /locus_tag="VCF75_000944"
     CDS             32..3247
                     /gene="rexB"
                     /locus_tag="VCF75_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEP2546520.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVVVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFLKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELISFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMGQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            3208..6876
                     /gene="addA"
                     /locus_tag="VCF75_000945"
     CDS             3208..6876
                     /gene="addA"
                     /locus_tag="VCF75_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEP2546521.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERYSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLCQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPAFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTDLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDIAHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGNVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNVFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QIPQLLNADDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKAAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKAKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALRGIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            7015..7827
                     /locus_tag="VCF75_000946"
     CDS             7015..7827
                     /locus_tag="VCF75_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP2546522.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAVQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLETPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
     gene            7968..8144
                     /locus_tag="VCF75_000947"
     CDS             7968..8144
                     /locus_tag="VCF75_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEP2546523.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(8272..8634)
                     /gene="mscL"
                     /locus_tag="VCF75_000948"
     CDS             complement(8272..8634)
                     /gene="mscL"
                     /locus_tag="VCF75_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEP2546524.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAV
                     APTQEELLTEIRDLLAQK"
     gene            8764..10578
                     /gene="dnaG"
                     /locus_tag="VCF75_000949"
     CDS             8764..10578
                     /gene="dnaG"
                     /locus_tag="VCF75_000949"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEP2546525.1"
                     /translation="MGFLWGGDDLAIDKEMIFQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNISSSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     ELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            10587..11696
                     /gene="rpoD"
                     /locus_tag="VCF75_000950"
     CDS             10587..11696
                     /gene="rpoD"
                     /locus_tag="VCF75_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEP2546526.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            11932..12270
                     /locus_tag="VCF75_000951"
     CDS             11932..12270
                     /locus_tag="VCF75_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEP2546527.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            12408..13262
                     /gene="rfbD"
                     /locus_tag="VCF75_000952"
     CDS             12408..13262
                     /gene="rfbD"
                     /locus_tag="VCF75_000952"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEP2546528.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            13381..14535
                     /locus_tag="VCF75_000953"
     CDS             13381..14535
                     /locus_tag="VCF75_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEP2546529.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEGNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWTK
                     QKGQLAELINQVDAGFDSDRLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            14525..15457
                     /locus_tag="VCF75_000954"
     CDS             14525..15457
                     /locus_tag="VCF75_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP2546530.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLNPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            15460..16263
                     /locus_tag="VCF75_000955"
     CDS             15460..16263
                     /locus_tag="VCF75_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546531.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGDVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYIVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            16263..17468
                     /locus_tag="VCF75_000956"
     CDS             16263..17468
                     /locus_tag="VCF75_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546532.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            17493..18500
                     /locus_tag="VCF75_000957"
     CDS             17493..18500
                     /locus_tag="VCF75_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP2546533.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTIYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEVSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            18497..20242
                     /locus_tag="VCF75_000958"
     CDS             18497..20242
                     /locus_tag="VCF75_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEP2546534.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMGPYFENFEEKEAVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKTESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSAFETNDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLLVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
BASE COUNT         6824 a         3399 c         3902 g         6273 t
ORIGIN      
        1 agggtagatt cagtttgtat aaaggagtca tatgaaatta atttacacag agatgagtta
       61 ctcgatgaca gagattttgg tgaatgaagc acgaaaagct gctgaccaag gttatcgagt
      121 gttctatatt gctccaaatt ctctttcatt tgaaaaggaa agagaagtct taaccttatt
      181 gcctgaacga gggacttttt cgataatagt tacccggttc gttcaaatgt caaggtattt
      241 tacagttgag agctctcctt ctaagcaaca tctagatgac acgactttgg ctatgatttt
      301 ttatcgggcc ttgatgcaat taaaaccaga agatttgccg agttatggtc gtttgcaaaa
      361 taattcggtt tttattgaac aactagttga gttatataag gaattaaaaa acgcacaatt
      421 aagcgtacat gatttaacag gattagacca tcctcaaaaa caagaagatc ttattaaaat
      481 tattgagctt gcagagacga tcatgattca gcaagattat aatcaagata gtcctctaca
      541 atcttttgca agagcaatta agttaggact tttaaataac caactatcaa aaacagttgt
      601 tgttattgat ggtttttcac gtttttcagc tgaagaggat tatttattat cacttttaaa
      661 taacaactgt caagaggtca tcattggcag ttatgtgagt caaaaagctt atcaaaaatc
      721 ttttattaag ggaaatatct atgaggctag ccttcatttt ttgcaggatt tggctcaaaa
      781 ataccatatc aagccggtat ttgcgacctc taatcaagtg tttaaaccag ccttttcacg
      841 gctaacacaa ttatttgaag caactcatga tttttcacaa gtagactggc aactccaaaa
      901 gaatgatctt gatcacttta gcctttggca atgtcatcat caaaaagagg aaattgaaca
      961 tgtagcaaaa tccattcggc aaaagctata tgaaggttac cgttataagg atatcttagt
     1021 gttgctagga gatatggatg cttatcaatt acaaattggg cctatctttg ataagtttga
     1081 aatcccctac tatttaggaa aagcagagcc tatggctgcc catccattag tccaatttat
     1141 agaatcgctc gaacgaagtc agcgctacaa ttggcgtcgt gaagacattt taaacatgtt
     1201 aaaatcagga ctttttggtt gttttgatga tagtgatatt gatcgctttg aggaatatac
     1261 tcagtttgca gatattaaag ggtttactaa attttcaaag ccttttacca ttaactcttc
     1321 taggcagtat cctcttgact ttttaaatga aatgagacaa gacatcgttt tacctttgca
     1381 agaattgttt aagagtcaaa aacaacttgg tgcttcactt gttgacaaat taatactttt
     1441 tttaaaaaag attagattag ctgagaatat gcaaggttta gcacagtccc aattagaggt
     1501 ggaaaaaaac gaagaagttt ggaaaaggtt tacagatatt ttgacttctt ttcatcatat
     1561 ctttggccaa gaaaaattac ggctatcaga ttgccttgct ttaatcaaaa cgggaatgaa
     1621 atcagctcag tatcgtgttg ttccagcgac attagatgtt gtcactatta aatcttatga
     1681 ccttgttcaa ccacattcaa aacctttcgt ttatgcaatt ggtctaactc aatcacattt
     1741 tcctaagcaa attcaccatt caggactact ttctgatcaa gagcgtgcta gaattaatga
     1801 aataagaaat tatcgtcatt ttgatattgc tagtgccgaa aatagtaaaa aaaaccatca
     1861 aactgcgtta tctctgttta atgctgctac taaagagtta gttcttagtg ttccaacggt
     1921 aatcaacgag acattcgatg atctatctcc ttatcttaaa gaattaataa gctttgggct
     1981 acctcttttg gataaaggca aaaactatct atcttatgat aatagcgata ttggtaacta
     2041 taaagctctc ttgtcacaaa ttattgctat taaccgacaa gatcttatag aaatgtcaga
     2101 tcaagataag atgttttgga ctgttgtctt acgttatctc aggaaacagt taagaaagca
     2161 acagctagag ttacctactt cagattatcg tttatcaaca aagcctttgt ctaaagaagt
     2221 tatcgaggta tgttttccaa agggaatccc tttaaagcta tctgcaactg ctttaaccgt
     2281 tttttataat aatcagtaca actatttttt gaaatatgtt ttgaatttaa acaaaacaga
     2341 atccattcat ccagattcca gaattcatgg tcaatacttg catcgtgtct ttgagcggtt
     2401 aatgaaagat cacacgcaag aaccgtttga taataagtta aaacaagcaa tctaccatac
     2461 caatcaagaa agtttttttc agcaggttta ccaagataat gccgaggctg agtacagttt
     2521 agctatacta gaagatattg ttagatcaac agcacctatc ttgcaactta accaaaacat
     2581 aaaggtcatt gatcaagaaa agaactttca tctggatatg ggaaatgaga tattagtcca
     2641 cgggatcatt gatcgtattg accagctgag cgacggtagc ttgggcattg ttgactataa
     2701 atcaagcgcg aatcaatttg atattgggac cttttataat gggttgagtc cacaactagt
     2761 gacttacctc gcagccttaa aacagatcgc accgcacgat ataaaccaat tatttggggc
     2821 tatgtacctt catttgcaag atcctaaatt ggatctagta acatttaaac aaattgataa
     2881 cacattagtg gagagtatct ataaagcgtt gacctataaa ggtatttttt cagaagtcga
     2941 aaaagagcat ctatctactg gtgcttacca aaccaaaaat gctctttatt ccaatgacga
     3001 attggaaaca cttttaaatt ataacaagta cctctatctt aaagctgcta aacacattaa
     3061 aaagggacat tttttgatta atccttatac aagtgatggt aagacagttc aaggagatca
     3121 attaaaagcc attacccgtt ttgaagcaga tcttgacatg ggacaggcaa ggcgactagt
     3181 gactctccct gctaaggaga aaaaagagtg ctttttaaca ttaatgagaa aggagagcca
     3241 cttgtgattt cttttgcccc atttttaagc cccgaagcta ttaaacattt acaagaaaac
     3301 gaaaggtaca gtgatcagtc tcaaaaacgc acagctcaac aaattgaagc aatttatact
     3361 agtggccaaa atatacttgt atcagcttct gctggttcag gaaaaacctt tgtaatggtc
     3421 gaacgcatac ttgataaaat tttgagaggt gtttcaattg atcggctttt tatctcaacc
     3481 tttactgtta aagcagctac agaactgcgt gaacggattg aaaacaaatt atactcacaa
     3541 attgctcaaa ctacagattt tcaaatgaaa gtttatttaa cagaacaatt gcaatctctt
     3601 tgtcaagctg atattggtac tatggatgct tttgcacaga aagtagtaag tcgctatggt
     3661 tatagcattg gcatttcatc ccaatttcgt atcatgcagg ataaagcaga acaagatgtt
     3721 ttaaagcaag aggtgtttag caaactcttt agtgagttta tgaatcaaaa agaggcaccg
     3781 gcgtttaggg ctcttgtgaa aaatttttct ggtaactgta aagacacttc agcttttaga
     3841 gagttagttt atacttgtta ttcttttagc caatcgacag aaaatccaaa aatatggttg
     3901 caagaaaatt ttctaagcgc tgctaaaact taccaaagac ttgaagatat cccggatcat
     3961 gatattgaac tcttactttt ggcaatgcaa gacactgcaa atcagctaag agatgtgact
     4021 gatatggaag attatgggca gctgactaag gcaggtagcc gatctgctaa atacactaaa
     4081 cacttaacga tcatagaaaa gttgtctgat tgggtgcgtg attttaaatg tttgtatgga
     4141 aaagccggat tggatcggtt gatcagagat gtgacagacc ttataccatc tgggaatgat
     4201 gttacagtct cgaaggtaaa ataccctgtt tttaagacct tgcatcaaaa attaaaacaa
     4261 tttaggcatt tagaaacaat tttaatgtat cagaaagact gttttccctt attggaacag
     4321 ttacaagatt ttgtgcttgc gttttcagaa gcttatttag ctgtcaaaat acaagaaagc
     4381 gcttttgaat tttcagatat tgcacacttt gcaatcaaaa ttttagagga aaatacggat
     4441 attcgccaat cctatcagca acactatcat gaggtgatgg ttgatgaata tcaagataac
     4501 aatcatatgc aagagcgact cctgacctta ctatcgaacg gtcataatcg ctttatggta
     4561 ggagatatca aacaatcgat ctatcgattt cggcaagccg atcctcagat ttttaatcaa
     4621 aagtttagag actatcaaaa aaaacctgag caggggaaag tgattttact caaagaaaac
     4681 tttcgtagcc aatcagaggt gttaaatgtc agcaacgctg tttttagtca cttaatggac
     4741 gaatcagtag gaaacgtctt atatgatgag caacatcagt taatagcagg tagtcatgct
     4801 caaacagtcc cctatctaga ccgtcgtgct cagttattgc tatataatag cgataaagat
     4861 gatggcaacg ccccttcaga tagtgagggt atttcattta gtgaggttac aattgttgcc
     4921 aaagaaatta ttaagcttca caatgataag ggtgtccctt ttgaagacat tacgttactc
     4981 gtttcttcaa gaacaagaaa tgatatcatt tctcatacat tcaatcaata tggtattcct
     5041 atagtaacag atggtgggca gcaaaactat cttaaatctg ttgaagtgat ggttatgtta
     5101 gatacattac gcaccattaa taacccaaga aatgattatg cccttgtggc tttactgcgc
     5161 tcaccgatgt ttgcctttga tgaggatgat ttagcaagaa tagcacttca aaaagacaat
     5221 gagctagata aagattgcct atatgacaag atacaaaggg ctgtgattgg aagaggtgct
     5281 catcctgaat tgattcacga taccttgctt ggcaagttaa atgttttttt aaagacgttg
     5341 aaaagctggc gtcgatacgc taagctaggg tcgttgtatg acttgatttg gaaaattttc
     5401 aatgatcgtt tttattttga ttttgtagct agtcaagcaa aagcagaaca agcacaagct
     5461 aatctatacg cattagctct acgtgctaat cagtttgaaa aatcgggcta taaagggctg
     5521 taccgtttta ttaaaatgat tgataaggta cttgagacgc aaaatgactt agcagatgtg
     5581 gaagtggctg ctcctaaaca agctgttaat ttaatgacca ttcacaagtc taaaggttta
     5641 caatttccgt atgtatttat ccttaattgt gacaagcgct tctcaatgac agatattcat
     5701 aaatcattta ttctgaatcg gcagcacggt atcggtatca agtaccttgc agatatcaaa
     5761 ggtttacttg gtgaaacaac actcaattct gttaaagtaa gcatggaaac cttaccttat
     5821 caattgaaca aacaagagtt acgcttagca actttatcag aacaaatgcg cttactgtat
     5881 gttgctatga cacgagctga aaaaaaagtt tattttattg gtaaagctag taagagcaaa
     5941 agtcaagata tcacagatcc taaaaagtta ggcaaacttt tgccgctggc tttacgagaa
     6001 cagttattga cattccaaga ttggctatta gcaatagcag atatattttc aactgaagat
     6061 ctttattttg atgttcgctt tattgaagat agtgatttga cacaagagtc agtcggacga
     6121 cttcagatac cacagttatt aaatgcagat gatcttaaag ataatcgtca atcagaaaca
     6181 attgcacggg ctttagatat gttagaagca gtgtctcaat tgaatgccaa ttatgaagca
     6241 gctattcatt tgccaacagt tcgaacgcct agccaactta aggcagctta cgagccttta
     6301 ttagaaccca ttggtgtaga tattatagag aaatcttctc gatcgctatc tgattttact
     6361 ttgccacatt tttcaaaaaa agcaaaagtt gaagcaagtc atattggatc agctcttcat
     6421 cagttgatgc aggtgctccc tttgtcaaaa ccgataaatc aacaaacgct tttagacgct
     6481 ttaagaggaa ttgatagtaa cgaagaggta aaaacagctc ttgatctcaa aaaaatagag
     6541 tcgttctttt gtgatacaag cctaggccaa ttttttcaga cttaccaaaa acacttgtat
     6601 cgagaagcgc catttgctat tttaaaagtt gaccctatta gtcaagaaga atatgtccta
     6661 cgtggtatta tagatgccta ctttttgttt gatgatcata ttgtattagt ggactataaa
     6721 accgataaat acaagcagcc cattgagtta aaaaagcgtt accaacaaca gttggagtta
     6781 tatgcagaag ctctcactca aacgtataaa cttcctgtga ctaagcgcta tcttgtttta
     6841 atgggaggtg gaaagccaga aattgtcgaa gtttaaggtt gttgtgacac aagggtattt
     6901 tgcaaatgat aatagtagag ttgaggattt tacgaaatgc ttgaaaagct attgaccttt
     6961 gaataataat acaatataat aagttttatt agcgaaaaaa ggaataaata tactatgaat
     7021 tttaaaaaaa taagatttgg ttttttgctt tgcctcctac tttatccttt agcagcttgt
     7081 aacaagtctg aacaacttaa tcactacgaa agaattaaaa aaacaagaaa attagtcgtt
     7141 gctgtaagcc ctgattatgc tccttttgaa tttaaagctt tagttaatgg aaaagatact
     7201 attgttggag cagatgttca attagctcag gctattgctg atgaattaga cgttgatctt
     7261 gagctttcac caatgagttt tgataatgtt ttgtctagtt tacagactgg taaagctgat
     7321 cttgctattt caggaatctc acatactaaa gaaagagcta aagtatatga tttttcaatt
     7381 ccttactatc aggcagaaaa cgctatagtg atgagagcat ctgatgctaa agtgaccaaa
     7441 aatattagtg acctaaacgg taagaaagta gccgttcaaa aaggtagtat cgaggaaggt
     7501 ttagttaaaa tacaattacc aaaggccaat ctgatttctt tgactgctat gggagaagcc
     7561 attaatgaac taaaagcagg acaagtctat gcagttacct tagaaacacc tgtagctgct
     7621 ggttttttag cccaacataa ggatctggct ttagcaccct ttagcttaaa aaccagtgat
     7681 ggagatgcca aagcagtggc ccttcctaaa aatagtggtg acttaaccaa agccgttaat
     7741 aaggttattg ctaagttaga tgagcaagaa cggtacaagt catttattgc agagacaata
     7801 gcactaacga aaaatactat gaagtaataa ggttaataaa aactttatta gtatccgttg
     7861 gaaaagtgtt tggaataaac aataaaagag gtttgacttt ttttaaaaaa taatgtaata
     7921 taattgggaa cgtgaaaatg ttacgatatc ttgaggaggt gaaacacatg tcaaaaacag
     7981 tagtacgtaa gaatgaatct cttgacgatg ctcttcgtcg cttcaaacgt tctgttacta
     8041 aagctggtac tcttcaagaa tcacgtaaac gtgaattcta cgaaaaacct tctgtaaaac
     8101 gtaaacgtaa atcagaagca gctcgcaaac gtaaaaaatt ctaatggtaa aaaataaaaa
     8161 cagttctcac aattagaact gtttttattt tttatccaga agaagcaagg gagctttttt
     8221 gatatcaaaa aaagcagtta gacatgccta actgctttat caaaaatcgc cttatttttg
     8281 agccaacaaa tcacggattt ctgtcaataa ttcttcttga gtaggagcta cagcttcttt
     8341 ttctttttta ggcatagctt tttcagcggc ttttacaaca aagaaaagac tggtaccgat
     8401 gataagaaaa ttaataacgg ctcctaaaaa gctaccatat ttaacaccat tccatgacaa
     8461 ttgagtgata ttttcaacat ttgcagcttt cagagctggg tttaaaataa gcggtgtgat
     8521 aatatcgttc acaaaagaag tgacaatagc accaaaagct cccccgatga taactgctac
     8581 agcaagttca ataatgtttc ctctaaacaa aaatgctttt aattctttaa ccattaaata
     8641 ttcccccttt taatatctct aagttaagca tagcataatt agtaaacaaa atccaatgga
     8701 atcacttcag tttttaaatt tacaaaaaag agtttcggtg gtataataaa acgtaaaact
     8761 aaaatgggat ttttatgggg aggtgacgat ttggcaattg acaaagaaat gattttccaa
     8821 gtaaaaaata gcgttaatat tgtcgatgtc attggagaag tggtcaaact ttcccgatca
     8881 gggcggcatt atctcgggct ttgcccattt cataaggaaa agacaccctc ttttaatgtt
     8941 gttgaagaca gacaattttt tcactgcttt ggctgtggaa aatcagggga tgtttttaaa
     9001 tttattgagg aataccgcca agtccccttc ttagaaagtg ttcagattat tgccgataag
     9061 actggtatgt cgcttaatat atcgtcaagt caggcagtac ttgctagcca acacaagcac
     9121 cctaatcacg ctttgatgac acttcatgag gatgctgcta aattttacca tgcagttttg
     9181 atgaccacta ccattggtca agaagctagg aagtaccttt accagagagg cttggatgac
     9241 caattaattg agcatttcaa tattggttta gccccagatg agtcagatta tctttatcaa
     9301 gctctttcta aaaaatacga ggaaggtcaa ttggttgctt caggattgtt taacttgtcc
     9361 gatcaatcta ataccattta cgacgccttt cgaaatcgta tcatgtttcc cttatcagat
     9421 gaccgagggc atattattgc cttttcagga cgtatctgga cggcagctga tacagaaaag
     9481 agacaggcca agtataaaaa ttcaagagga acagttcttt ttaacaaatc ttatgaattg
     9541 tatcatctgg acaaggcaag gcctgttatt gccaaaaccc atgaagtatt tctaatggaa
     9601 gggtttatgg acgtgattgc cgcttaccgt tccggctatg aaaatgctgt tgcttcaatg
     9661 gggacggcat tgactcaaga acatgtcaat caccttaagc aagtcactaa aaaagttgtt
     9721 ttgatttatg atggtgacga tgctggacaa catgctattg caaaatcact agaattgctt
     9781 aaagattttg ttgtcgaaat tgtcagaatc cccaataaaa tggatcctga cgaatttgta
     9841 caacggcatt ccccagaagc atttgcagat ttgcttaagc agtcacggat cagtagtgtt
     9901 gaatttttta ttgattacct aaaacctact aatgtagaca atttgcaatc acaaattgtt
     9961 tatgtggaga aaatggcacc attgattgct caatcaccat ccatcacagc tcaacattcg
    10021 tatattaaca agattgctga tttgttgcca aactttgact attttcaagt agaacaatca
    10081 gtaaatgcat taaggattca agataggcaa aagcgtcaag gtcaaatagc tcaagctgtc
    10141 agcaatcttg tgaccttacc aatgccaaaa agtttgacag ctattgctaa gacagaaagt
    10201 catctcatgc atcggctctt acatcatgac tatttattaa atgaatttcg acatcgtgat
    10261 gatttttatt ttgatacctc taccttagaa ttactttatc aacggctgaa gcaacaagga
    10321 cacattacat cttatgattt gtcagagatg tcagaggaag ttaaccgtgc ttattataat
    10381 gttttagaag aaaaccttcc caaagaagta gctcttggtg agattgatga tattttatcc
    10441 aaacgtgcca aacttttagc agagcgcgat cttcacaaac aagggaaaaa agttagagaa
    10501 tctagtaata aaggcgatca tcaagcggct ctagaagtac tagaacattt tattgcgcag
    10561 aaacgaaaaa tggaatagag gaaattatga caaaacaaaa agaaataaca acttttaacg
    10621 ttcaagttgc ggagtttatt cgccatcaca aaaaagaagg aacagcgatt gatgatgacg
    10681 tgaccgaaaa acttgtcatt ccatttgctt tggatgctga tcagattgat gacttacttg
    10741 agcggttaac agatggtggt atctcgatta cggataaaga aggtaaccca tcatcaaaat
    10801 acattgttga agaaccaaaa ccagaagaat taactgatga agaattaatt ggtagtaatt
    10861 cagctaaagt aaacgaccct gtccgcatgt acctcaaaga aattggtgtg gtaccgcttt
    10921 taacaagtga agaggaaaaa gaattggctg ttgcggttgc caagggagat ttaatggcta
    10981 aacaacgttt agcggaagca aaccttcgtc ttgttgtctc aattgcaaaa cgttatgttg
    11041 gacgtggaat gcagtttctt gatttaattc aagaaggtaa catgggactc atgaaagctg
    11101 ttgataaatt tgattattca aaaggattca aattctcaac ttatgcaaca tggtggattc
    11161 gtcaagctat aacacgcgca attgcagatc aagcacgtac gattcgtatc cctgttcata
    11221 tggttgaaac gattaataaa ttagtccgtg aacaacgtaa ccttcttcaa gaacttggtc
    11281 aagatccaac gccagaacaa atcgcagaac gtatggaaat gactccagac aaggttcgtg
    11341 aaattctaaa aattgctcaa gaacctgttt ctttagaaac accaattggg gaagaagatg
    11401 atagtcactt aggtgatttt atcgaagatg aagttattga aaatccagta gattatacga
    11461 cgcgtgttgt tcttcgtgag caattggatg aagttttaga tacgcttact gaccgtgaag
    11521 agaacgttct tcgcctacgc tttggccttg acgatggtaa aatgcgtacg ttagaggacg
    11581 taggtaaagt ctttaatgtt actcgtgagc gcattcgaca gattgaagct aaagccctta
    11641 gaaaacttcg ccatcctagt cgtagtaaac aattaagaga ctttatagag gactaaaatc
    11701 gtccagtgga cgattttagg tcgttccttt gaaataagaa aggagcgaaa atcccaagtg
    11761 gcacttatca gcggtacacc tacaaattag aaaacggacc gaatgtggtg ggtattttta
    11821 agggtagact tgatatctta aacaagccca gagggcaatt cctctaaata ggcaaagctt
    11881 tagtggtttt tttacttatc tacgacatat tcaaaaaatg aaaggaaagc catgtctgat
    11941 acaccaaaat atacacaaga tcaggtgata gctatcaaga atagaattct tgaagcttta
    12001 gaaactgtta ttgatcccga gctaggtatt gatgttgtta atctaggctt gatttatgaa
    12061 attcggttta atgataacgg ttatacagag attgatatga cactcacaac aatgggttgt
    12121 cctttggcag acttgttgac agattacatc catgatgccc tgcaagatgt gccagaagtc
    12181 actaagactg aggtcaaatt agtctggtac cctgcttgga cggttgataa aatgagccgc
    12241 tatgctagga ttgcgctggg tattcgctaa tcattgatag taaagggatg aaatattccc
    12301 ttttttgatg tagaataaaa ggaaatacag gtgaaagcct ttggttaatg ttagagaaca
    12361 ctaagatact gagaaaagtg ttctacctat ggcaaaaagg agaactgatg attttaatta
    12421 caggaagcaa tggtcaatta gggacagaac ttcgatactt attagatgaa cgaggtgttg
    12481 attatgtagc cgttgatgtc gcagaaatgg acatcacaaa tgaagacaaa gtcgaagctg
    12541 tttttgcgca ggtcaagcca acattggtct atcattgtgc tgcttatacg gcagttgatg
    12601 cagcagaaga cgaaggaaaa gccttgaatg aggctattaa tgtaacaggc tcagaaaata
    12661 ttgccaaggc ttgtggaaaa tatggggcta cccttgttta catttcaaca gactacgtat
    12721 ttgatggcaa taaaccagtc ggtcaagaat gggtagaaac agatcgtcca gaccctaaga
    12781 cagaatatgg ccgtacaaaa cgtttaggag aactagctgt tgaacgatac gcagagcatt
    12841 tttatattat ccgtactgct tgggtatttg gaaattatgg taaaaacttt gtctttacca
    12901 tggaacagtt agcggaaaat cactcccgtt tgactgttgt caatgaccaa cacggtcggc
    12961 caacgtggac aagaaccttg gcagaattca tgtgttattt gacagagaat caaaaagcct
    13021 ttggttacta tcacttgtca aatgatgcta aggaagacac cacttggtat gattttgcca
    13081 aagaaatctt aaaagacaag gctgttgaag tggtgccagt tgattcatca gctttcccag
    13141 caaaagccaa acgtccttta aattcaacca tgaatcttga taaggcaaaa gcaacagggt
    13201 ttgttattcc aacatggcaa gaagccttaa aggcatttta ccaacaagga ctgaaaaagt
    13261 aagtattcta aaaagagttg gctttctaag gccaactctt tttcatgggt ttaaggtcgc
    13321 attagccttc taattgtggt ataatatcac tgattatatt ttagaatggg gtagtatgca
    13381 atgcaggatg tttttatcat tggtagcaga gggttaccag cgaaatacgg tggctttgaa
    13441 acctttgtcg aagagttgat tagtcatcaa tcatctaaaa acattcgtta tcacgttgct
    13501 tgtttaagcg atacgaagca taaggttcat tttgattata agggggcaga ttgcttttac
    13561 cttaatcccc caaaacttgg tcctgctaga gttattgctt atgatatgat ggcaattacc
    13621 tatgccctgt cttatagcga ccaacaccag attcagaacc ctatttttta tgtgttgggc
    13681 aatactgtag gcgcttttat tgcgccattt gtgaagcaga ttcacaatcg aggtggacgc
    13741 ttttttatca atccagacgg gttagagtgg aaacggtcta aatggtcaaa accagtgcag
    13801 gcctacctga agttttccga aaagcaaatg actagacagg ctgacttggt catttcagat
    13861 aatattggca ttgaccgcta ccttaaacag gtttatcctt ggtctaaaac atgttttatt
    13921 gcttatggaa cgcagacaca accttcacgt ttgacaacag cagatagcaa ggtcagagct
    13981 tattttcaaa cctttgatat tagagaaaag gattactatc tcattttagg acgctttgtt
    14041 cctgaaaata attacgaaac tgctattaaa gaatttatgg cttcctcaac taagcgtgat
    14101 ttagtgatta tttgtaatca tgagggcaat gcttacttta agcaattact tgctgagaca
    14161 gagtgtgata aagacccacg cattaaattt gttggtaccc tttatgacaa agaactatta
    14221 gcctacattc gagaacaggc ttatgcctat atccatggtc atgaggtagg agggactaat
    14281 ccaggccttc tagaagcact agcccatacc aatcttaatt tggttttagg agttgatttt
    14341 aaccagtcgg tggctaagtc tgctgcgctt tattggacca agcaaaaagg gcaattagca
    14401 gagctgatta accaagttga tgcaggtttt gatagtgatc gtctgggcaa agaagccaaa
    14461 gctattattc aagaacatta cacttgggaa aaaattgtag gggagtacga ggctttattt
    14521 ttaaatgaac attaatattt tactatccac ctacaatgga gaacgcttct tagcagaaca
    14581 aattcaaagt atccaaaggc aaacagttaa cgactggacc ttattgatta gagatgatgg
    14641 ttcaactgat ggcacccagg atattattcg cacatttgtc aaggaagaca aacgaattca
    14701 gtggatcaat gaggggcaga cagaaaatct gggagttatt aagaattttt acaccctctt
    14761 aaagcaccaa aaagcagatg tctatttttt cagtgaccaa gatgatatat ggttagacaa
    14821 taaactggaa gtgaccttat tggaggctca aaaacatgaa atgacagctc ctttattggt
    14881 ttatacagac ctcaaagtgg taacccaaca tttagctatt tgccatgaca gtatgattaa
    14941 gacccaatct ggtcatgcaa acacaagcct acttcaagaa ttaaccgaaa ataccgtaac
    15001 aggcggcaca atgatgatta ctcatgctct ggctgaggaa tggaccactt gtgacggttt
    15061 attgatgcat gactggtatt tagctttact agcttctgct actgggaaat tagtttacct
    15121 tgatattcca acagaattat accgtcagca cgatgctaac gtgttgggag ctagaacttg
    15181 gtctaaacgc atgaaaaatt ggttaaaccc ccatcattta gtcaacaaat attggtggtt
    15241 gattacttca agccaaaaac aggctcaatt actattagat ttaccattaa aaccaaatga
    15301 ccatgaattg gtaacggcct atgtttcttt attggatatg ccttttacca aacgcttagc
    15361 taccttgaaa aggtacggtt ttagaaaaaa tcgcatcttt cataccttta tttttcgtag
    15421 tttagttgtt accttatttg gttacaggag aaagtaagaa tgaatttttt aacaaagaaa
    15481 aaccgtattt tactgagaga aatggtaaaa acggatttta aattacgcta tcaagggagc
    15541 gcaattggct atctatggtc tatcttaaaa ccgcttatga tgtttacgat tatgtacttg
    15601 gtatttattc gttttcttcg cctgggtgga gatgtacctc attttccagt agcgctttta
    15661 ttggcaaatg tcatctggtc ttttttttca gaagcaacta gcatgggaat ggtatctatt
    15721 gtatctcggg gagacttgtt gcgaaaattg aacttttcta agcacatcat tgttttttcg
    15781 gcagtgttag gagctttaat taattttctt attaatttgg ttgttgtttt aatttttgcg
    15841 ttgataaatg gtgtgactat atcagggtat gcttatctct ctctttttct ttttatagaa
    15901 ttagttgttt tagtgcttgg aattgcattg ttattgtcga atgtctttgt ttattatcgt
    15961 gacttagctc aagtctggga agtactatta caagcaggta tgtatgccac tccaatcatt
    16021 tatccgatca cttttgtttt agatagccac cctttggcgg caaagttgtt gatgctaaat
    16081 ccagtagcac aaatgattca agattttcgt tatttattga ttgacagagc caacgtaacg
    16141 atttggcaga tgtcaactaa ttggttttac attgttattc catatttagt accatttgtt
    16201 atattattta ttggcatctt tgtctttaag aaaaatgccg atagatttgc ggagattatt
    16261 taatgattaa tagagaaatt gcagtaaaag tacagcatgt tagtaaaaca tttaaactgc
    16321 ctacagaagc aactaaaagt tttcgcacga ctctggttaa tcgcctaaga gggattaaag
    16381 gatttactga acaacatgtg ctaaaagata ttaactttga tgtttataaa ggcgattttt
    16441 ttggaattgt tgggcgtaac ggctcgggaa aatcaacgct ccttaaaatt atttcccaga
    16501 tctatgttcc agaaaaagga caggttacag tagatgggaa gatggtttct tttatagagc
    16561 ttggagtggg attcaatcca gaattaacag gacgagaaaa cgtctatatg aacggcgcca
    16621 tgctgggatt taccaaagac gaagttgatg atatgtacaa tgatattgtt gattttgcag
    16681 agttgcatca ttttatgaat caaaaattaa aaaattattc ttctggcatg caggtacgtt
    16741 tagcattttc agtggctatt aaagcccaag gtgacgttct tattttagat gaggtgcttg
    16801 ctgtaggaga tgaagctttt cagcgaaaat gtaatgatta ttttatggaa agaaaagaca
    16861 gtggtaagac gactatttta gtgacacatg atatgggagc tgttaagaag tattgtaacc
    16921 gtgcagttct tattgaagat ggtcttgtta aagcatatgg agaaccattt gatgttgcta
    16981 atcagtatag tgtagataat acagagacag ctgaggatgc aatgaatgct gaaaaaatat
    17041 ctgtatctga tattgccaaa gatttgaaag tgtcattaat ctcaaatcca agaataacac
    17101 caaatgacac aatcacattt gaagtttctt atgaagtttt aaaagatgat tttacctaca
    17161 ttgccttttc tttaacggat attgatagaa acatttgggt ttacaacgac aattctttag
    17221 attatccaac acaaggtata ggtagaaaat gcatttccta ccaatgtcaa ctatctcaac
    17281 ttaatgatat aaaactaaaa ttagaagtaa ctgtccgaga taagaacggt cagatgctac
    17341 tcttttcaac agcagaacaa tcaccccaga tagtgattca aagaaacgat attgatcaag
    17401 atgatctgtc agctctagat tcagcaagtg gcttgtatca acgtaatggg aagtgggttt
    17461 ttcattaatg agtaatagct agaagggaat ttatgtataa agtttcaatt atttgcacca
    17521 attataacaa agctccttgg atatctgatg cacttgacag ttttttaagc caagtgacag
    17581 attttgaagt tgaaattatt gttattgatg atgcttctac agatgactca cgagagattt
    17641 taaagagtta tcaaaaaaag tcttcaggaa aaattaaatt actctttaac gaaactaata
    17701 ttggaataac aaagacttgg ataaaagcat gcctttatgc aaaaggcaaa tatattgcta
    17761 gatgtgatgg tgatgattac tggacggata gttttaaact ccaaaaacaa gttgatgtct
    17821 tagaagcttc caaacggtca cgatggtgta atactgattt tgactttgta aatagtcaag
    17881 gcgacttatt atatgctaat gctttttcat ctggtcatac acctttgaca gacacttacg
    17941 aaaaagtttt agccttaaaa gggatgacga tggcatcgac atggttagtt gatgctgatt
    18001 taatgagaca agtaaaccaa aaaatcaatg tagatacacc agacgatact tttgatattc
    18061 aactagagtt atttcaactg actcaactga cctatattaa agactcaacg acgatttatc
    18121 gcatgacaac cgattctgat tcaagaccta ctgatacacc aaaaatgatt catcgtattc
    18181 aaaaactact tgatactcag ttaaattact taaaaaagta taatcaggta gatacaaaag
    18241 aagtgtctga actattatta caacaagatg ctaagcaaga aatacgaatt catgagttga
    18301 gttgctttat tcaagaatta caacaaacta tcgtagataa aacaaagcag caagaaacac
    18361 gtgaagtgga gttgcaaaat gtcatagaag aacaaaaaaa tcaactatct gaattaagac
    18421 aacaatacca tgccattatt aattcacgtc aatggaaata cacgtctaag ctcattgctt
    18481 ttattaggag aaaaaaatga atcgtatttt attatatgtt cacttcaaca aatacaataa
    18541 aataagtgca catgtttact atcaattgga acaaatgaga tcactgtttt ctaagattgt
    18601 ttttatctca aatagtaaag tgagtcatga agatttaaaa cgtctcaaaa accactgtct
    18661 tattgatgag tttttgcaac gcaaaaataa aggttttgat ttttctgcat ggcacgatgg
    18721 cttgattact atgggatttg acaagttgga agagtttgac tcattgacga ttatgaacga
    18781 tacttgtttt ggtcctattt gggagatggg tccttatttt gaaaattttg aagaaaaaga
    18841 ggcagttgac ttttggggaa ttactaataa tagaggtaca aaagctttta aagaacatgt
    18901 ccaaagttat tttatgacgt ttaaaaatca agtcattcaa aacaaagttt ttcaacaatt
    18961 ttggcaatcg ataatagaat atgaaaacgt tcaagaagtg attcagcatt acgaaacgca
    19021 attaacatcc attctcttaa atgaaggctt tagttatcag acggtctttg atactagaaa
    19081 aactgagtcg tcattcatgc cacatccgga tttttcatat tataatccaa cagcaatttt
    19141 aaaacaccat gtgcctttta taaaagtaaa agctattgat gctaaccagc atatcgctcc
    19201 ttacttactg aatctgatac gagaaacaac caattatcct atagatctta ttgtttcaca
    19261 tatgtcgcaa atctctttgc cagatgctaa gtacctttta tctcaaaaat acctaaactg
    19321 ccaacggtta gcaaaacaaa cttgtcaaaa ggtagctgtt catttacatg ttttttacgt
    19381 tgatttgcta gatgaatttt taacagcttt cgaagactgg aacttccatt atgatctttt
    19441 tattacaaca gatagtgata tcaaaagaaa agagatcaaa gaaatacttc aaagaaaagg
    19501 taaaactgct gatatacgcg ttacgggaaa tagaggaaga gatatttatc caatgttatt
    19561 actaaaagat aaactttctc aatatgatta tattggtcat ttccatacga aaaaatcaaa
    19621 agaagctgat ttttgggcag gagaatcttg gcgaaaagaa ttgattgata tgctcgtgaa
    19681 accagcagat agtattctta gcgcttttga gacaaatgat attggtatta taatcgcaga
    19741 tattccaagt ttttttagat ttaataagat tgtcaatgct tggaatgagc atttaatcgc
    19801 tcaagaaatg atgtctttgt ggagaaaaat ggatgttaaa aaacaaattg attttcaagc
    19861 tatggataca tttgttatga gttatggaac atttgtatgg tttaaatacg atgctctaaa
    19921 atcacttttt gatttagaat taactcaaaa tgatattcca agtgagcctt taccacaaaa
    19981 ctctatatta catgcgattg agcggttact tgtctatata gcgtggggga attcttatga
    20041 ttttaggatt gttaaaaatc cctatgagtt aacacctttt attgacaata aactgttaaa
    20101 tttacgtgaa gatgaaggag ctcataccta cgttaacttt aatcaaatgg gcggtatcaa
    20161 aggagcatta aaatatatta tcgttggtcc cgctaaagca atgaagtata tattcttgcg
    20221 tttaatggag aaacttaaat gattaaagac acatttttaa aaaccaattg gttaaatatt
    20281 agccaccata ttacccttct tgttttcggt ttttatttca gtttttatag tttggcgaaa
    20341 gaactagtaa gctccacggc acaaccggta aactattata ctcatttact aaatgttt
//
LOCUS       DATURV010000027        20325 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DATURV010000027 DATURV010000000
VERSION     DATURV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20325
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.027"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..787)
                     /locus_tag="VCF75_000959"
     CDS             complement(<1..787)
                     /locus_tag="VCF75_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2546535.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSF"
     gene            1031..2278
                     /locus_tag="VCF75_000960"
     CDS             1031..2278
                     /locus_tag="VCF75_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP2546536.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            2552..3913
                     /locus_tag="VCF75_000961"
     CDS             2552..3913
                     /locus_tag="VCF75_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2546537.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFTYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            3913..4749
                     /locus_tag="VCF75_000962"
     CDS             3913..4749
                     /locus_tag="VCF75_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2546538.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            4907..5704
                     /locus_tag="VCF75_000963"
     CDS             4907..5704
                     /locus_tag="VCF75_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2546539.1"
                     /translation="MTSKITIKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHI
                     SNLLNYTPNQAARSLVTQQSNSVVLYYKYADSILARAEQLHTISFLSRLLHEQDYQLV
                     CLNEDSIGKFDWADLILTLDIENDTFYKIGKLNFKPLIAIDSFVNDPLFFQINTDYAN
                     LKDQADQFFKDEPYTLLTLPINNQERYEAISTLFPNLHVVDHMRELPSFEKKNLLLID
                     ESLDQFFVNSSQKLYCPRFSPEKGQVILEAIEWAKQRQLNKNHNLLV"
     gene            complement(5889..6020)
                     /locus_tag="VCF75_000964"
                     /pseudo
     CDS             complement(5889..6020)
                     /locus_tag="VCF75_000964"
                     /inference="COORDINATES: protein motif:HMM:NF037788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6626..7486)
                     /locus_tag="VCF75_000965"
     CDS             complement(6626..7486)
                     /locus_tag="VCF75_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2546540.1"
                     /translation="MRKTYVSTSMKRKRFFTQLLTYLYLISLAIVILFPILVTVSSAF
                     RPGNTTAFSFHFDGPWTLSNFKTLFQDTLYLRWYWNTLIVAFFTMLIQVTVITLTGYA
                     YSRYNFFGRKKSLIFFLVVQMVPTMAALTAYFVMAWLFNALNQYWFLILIYVGGGIPM
                     NAWLMKGYFDTVPYDLDESAKLDGSGHFRTFYQIVLPLVRPMIAVQSLWAFMGPFGDF
                     MLAKFLLRAQENYTVAVGLQSFITNDARNPKVTLFAAGAILIAVPISVLFFFLQKNFV
                     SGLTSGGTKG"
     gene            complement(7486..8790)
                     /locus_tag="VCF75_000966"
     CDS             complement(7486..8790)
                     /locus_tag="VCF75_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2546541.1"
                     /translation="MVDNQRQQKPNKAMWLSVIPGLGQLYNKQIVKGGVLLVVFFLEL
                     LEIVVLGIPALTGLYSLGNVPMQDHSLFMLIKGAMQLITLVLFGIFHLVAMRDAKLVA
                     HQMNEGKKVPVTAKETLEAIYEKGFPYLLIIPAYLAMAFAIIFPVLVTLLIAFTNYDF
                     RHIPPYRLLDWVGLKNFLNILELSTFKTAFTSVLSWTLIWTLAATSLQIVIGIATAVI
                     ANQSFIRGKRLFGVIFLLPWAVPAFISIMSFGNFFNDSIGAMNVQVLPFIEKFLPFVD
                     FGIVSWKTDPFWTKVAVIMVQGWLGFPYIYILVSGILQAIPADLYEAATVDGVTVLQK
                     FRHITMPMIFAVAAPTLIAQYTFNFNNFSIIYLFNNGGPGTVGGGAGTTDILISWIYK
                     LTTQTSPQFSMASAVTLIISVIVISVSLITFKKFKAFDMEER"
     gene            complement(8865..11000)
                     /locus_tag="VCF75_000967"
     CDS             complement(8865..11000)
                     /locus_tag="VCF75_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrin glucanotransferase"
                     /protein_id="HEP2546542.1"
                     /translation="MRELHIKTYKLLTKSAVLLGLISFPLTVSAADNASVTNKADFST
                     DTIYQIVTDRFNDGNTSNNGKTDVFDKNDLKKYHGGDWQGIIAKIKDGYLTDMGISAI
                     WISSPVENIDSIDPSNGSAAYHGYWAKDFFKTNQHFGTEADFQQLVKVAHQHHIKVVI
                     DFAPNHTSTAEKEGTTFKEDGALYKNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYH
                     SMYGLADLNNINPKVDQYMKEAIDKWLDLGVDGIRVDAVKHMSQGWQKNWLSHIYEKH
                     NVFVFGEWFSGHTDDDYDMTTFANNSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVL
                     ENRDQVTNEVTDQVTFIDNHDMERFATKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ
                     YATGDKDPNNRGDMPSFNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFE
                     RKFGNHVALVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSF
                     ELGAGQVAVWTYEGEDKTPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEI
                     LSWSDTLITLKVPTVPANYYNISVTTADKQTSNSYQAFEVLTDKQIPVRLLINDFKTV
                     PGEQLYLMGDVFEMGANDAKNAVGPLFNNTQTIAKYPNWFFDTHLPINKEIAVKLVKK
                     DSIGNVLWTSPETYSIKTGHEAQTITIKK"
     gene            complement(11026..12729)
                     /locus_tag="VCF75_000968"
     CDS             complement(11026..12729)
                     /locus_tag="VCF75_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEP2546543.1"
                     /translation="MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGP
                     KYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTT
                     SYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKDDSYVNMGW
                     LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQF
                     GTKYDLQELIDLAHQMGIKIILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEH
                     PSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHD
                     FWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTS
                     KQLAEKLSNILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGI
                     PCLYYGTEVAMEGGYDPDSRRGFPWDDAERDKDFFSKVKAIIALRQQPALQEGAISID
                     YDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQDGYIVFS
                     N"
     gene            complement(12895..14154)
                     /locus_tag="VCF75_000969"
     CDS             complement(12895..14154)
                     /locus_tag="VCF75_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP2546544.1"
                     /translation="MSWNWKKTSVLGTLSLASVLPLTACVSGGGKGVKETDGKTIVVS
                     VDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRV
                     GGLGTEGQIAEVTLGNSKEFDDTVKKLVTIDGKTYGAPDVIETLVTYYNKDLVPQAPK
                     SFTELEVLQKDSKFAFASEPGKSVGFLAKWTDFYYGYGLIAGYGGYIFGDKGTKPSDL
                     GLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASSFK
                     DAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY
                     DKTQEIPANLTARNYASKEGNELTKAVISQFENAQPMPNIPEMAEVWEPGANMFFNVA
                     SGKEEASKAAKEAAKTIKEAIEQKYAE"
     gene            complement(14774..15757)
                     /locus_tag="VCF75_000970"
     CDS             complement(14774..15757)
                     /locus_tag="VCF75_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP2546545.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEHT"
     gene            complement(15788..17038)
                     /gene="dltD"
                     /locus_tag="VCF75_000971"
     CDS             complement(15788..17038)
                     /gene="dltD"
                     /locus_tag="VCF75_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEP2546546.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(17031..17270)
                     /gene="dltC"
                     /locus_tag="VCF75_000972"
     CDS             complement(17031..17270)
                     /gene="dltC"
                     /locus_tag="VCF75_000972"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEP2546547.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(17288..18544)
                     /gene="dltB"
                     /locus_tag="VCF75_000973"
     CDS             complement(17288..18544)
                     /gene="dltB"
                     /locus_tag="VCF75_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEP2546548.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKAFMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(18541..20079)
                     /gene="dltA"
                     /locus_tag="VCF75_000974"
     CDS             complement(18541..20079)
                     /gene="dltA"
                     /locus_tag="VCF75_000974"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEP2546549.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGERLTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELSSGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(20091..20234)
                     /locus_tag="VCF75_000975"
     CDS             complement(20091..20234)
                     /locus_tag="VCF75_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEP2546550.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         6511 a         4138 c         3408 g         6268 t
ORIGIN      
        1 aaaaagataa taactgtacc atacgaatag ccagaacatc accaacaaca accaaggcag
       61 ttgctttaaa ggaagatata gtttccatca atatttctac gctaacagga tccttacgat
      121 caaataacag cattggcttc gtttttaaag atcgcttaaa acaccccttc aaatagccaa
      181 gataacgctc agaagttact tctgatagta aatcatctgt tataaataat atgttgtgat
      241 ggcctttttg ataaaggtgt tctactgctg ttttagccat tagctgatta tcattatcaa
      301 tataagtggt tttattagtg tcaccttgtg gagcacctac aatgacaaat ggtaagttat
      361 ttgtcatcaa atattttttg acaggatcat cttgatcaga ataaagaata ataaaaccat
      421 caactcgttt ttgaagatgc atcagtttca cttgctctat caggtcatca agagatatac
      481 ctgtagcaat cgacacagta aaatgagatt ctttagcttc gttagtaata gtcgacaaaa
      541 tttccataaa gaaaggttca cttaatctat cgctagttgt aataggagga aaaacaagtc
      601 ctatattgtg tgtcaaacca ctcgctagaa tctgtgctgc gacatttgga acatatccca
      661 aatctgccat cgcttttcta accttctctt ttgttttcat agaaatagat cgattatctt
      721 ttaataccct actcaccgtc gaagggttaa cacctgcctt ttgagcgaca tcttttattg
      781 ttaccatatt aacctcgtta ctatatttac caaagataaa ccttatcatg ggtttgtcat
      841 gtatttcgtt tccattatac aattatcttt caaaaaatca acttcaatca agattccctt
      901 gtcattttaa ctcaaattct ctaaactatt tttttaaaaa aacgaccttt tttttaaaaa
      961 aggcttgact ttttctgcaa gcggttgcat aatattaagt gtaagaaata tatcttagga
     1021 ggaaatagct atgaaatcat ggcaaaaagt tatcgtcggc ggagcaagtt tgacacttgc
     1081 aagtaccttg ttagtgggat gtggatcagg ttcaaaagat aaaaaggaag ctggcgctga
     1141 ctcaaagact attaaacttt gggtaccgac tggctctaaa aaatcatatg cagataccat
     1201 tgcaaaattt gaaaaagatt ctggttacac cgttaaagtg gttgaatcag aagatccaaa
     1261 agcacaagaa aaaatcaaaa aagatgcctc aactgctgcc gatgtctttt cacttcctca
     1321 tgatcaactt ggtcaacttg tagaatctgg tactattcaa gaagttccag aaaagtacaa
     1381 taaagaaatt gctgctactt ctactgatca ggcgctcgtc ggtgcacagt acaaagggaa
     1441 aacatatgct ttcccatttg gtattgaatc acaagttctt ttctacaata aatcaaaatt
     1501 agctgctgaa gacgttactt catatgatac aattactaca aaagcaactt ttggtggtac
     1561 ctttaaacaa gctaacactt acgctactgg tccattattc atgtcagttg gtaacacttt
     1621 atttggggaa aacggtgaag atgtaaaagg tactaactgg ggcaatgaaa aaggcgctgc
     1681 tgttcttaaa tggatcgcag accaagcttc aaataaaggt tttgttagct tagatgctaa
     1741 caacgttatg tctaaatttg gtgacggttc agtagcttct tttgaatcag gaccgtggga
     1801 ctatgaagct gctcaaaaag ctatcggtaa agaaaacctt ggtgttgcta tttatccaaa
     1861 agtaactatt ggaggcgaaa cggttcaaca aaaagcattc ttgggtgtaa aactttacgc
     1921 agtcaaccaa gcaccagcta aaggtgatac aaaacgtatc gcagctagct acaaattggc
     1981 atcctacttg actaatgctg aaagccaaga aaaccaattc aagactcgta acattgtccc
     2041 agccaacaaa gaagttcaat cttcagaagc tgttcaatca aacgaacttg ctaaaaccgt
     2101 tatcactatg ggttcttctt cagactacac tgtcgtaatg ccaaaactta gccaaatggg
     2161 cacattctgg actgaaagtg ctgctattct tagtgatgcc ttcaacggta aaatcaaaga
     2221 aagcgactac cttactaagt tgcaacaatt cgacaaagat atcgctgcaa caaaataata
     2281 tgatctcatg tcatcactgc tatactaaaa caagcacaag gtaagtactt ctaaaaagtt
     2341 ctttccactt accttatgaa gagtggaact aggctccatc acattttatt caagcttagt
     2401 ctcactctta cttgtttttg ttttactagt agcttagtgc tacttaaatt tttaactctt
     2461 taaaagttta cgtaacactt agctatttca tcctaacaag agattgaatc tgaactacca
     2521 ctctagtatc ctaaaataaa ggagaatcta tatgactcag tcaaacttga caccaaatat
     2581 atctgtaata gaagccttaa aaagaggcag ttgggatatt aaactttcga gtattatcat
     2641 gggatttgcc aactttgcta acaaacagtt tatcaaagga atcttatttt taataagtga
     2701 acttatcttt ctagtagctt ttgtctccca aatcatccct gctatccgtg gccttgtcac
     2761 tcttggtact caaactcagg gaatgactac aaaaactatt gatgggatta acatccaagt
     2821 tgctgttgat ggcgataact ctatgctaat gcttattttt ggtttagcat cactcatttt
     2881 ttgcctagtc tttacttaca tttactggtg taatctcaaa agtgctcgta acctttatct
     2941 tttcaaacaa aaaggtcaga agataccaag cttcaaagaa gatcttgcca cacttacaaa
     3001 tggtcgcttt catatgactt taatggctat cccactgatt ggtgtgcttt tattcaccat
     3061 tcttcctttg atttacatga tctgtttggc ctttaccaat tttgatcata atcacttgcc
     3121 tccaaaatct ttatttgatt gggtgggact tgctaacttt ggaaatgttt taagcggccg
     3181 tatggcagga accttttttc ctatcttctc atggactttg atttgggctg tttttgccac
     3241 agtaactaat ttcttctttg gtattattct tgctttgtta atcaatacca aaggattaaa
     3301 gtggaaaaaa atgtggcgaa ccatctttgt tatcaccatt gcagttccac aattcatctc
     3361 attattgatt atgcggaact tactcaatga cgaaggccca ctaaatgctc ttctcaataa
     3421 aatcggcttg attaatggct cactgccatt tttatctgat cctctttggg caaaattctc
     3481 aatcattttt gtcaacatgt ggatcggtat tccatttacc atgttgattg cgactggtat
     3541 tatcatgaac cttccaagtg aacaaattga ggctgctgaa attgatggcg ctagcaaatt
     3601 ccaagtcttc aagtctatca cattccctca aattctcttg atcatgacac caaacttgat
     3661 tcaacaattt atcggaaata tcaataactt taacgtcatt tacctcctta ctggtggtgg
     3721 tccaacaaat tcagaatact atcaggcagg aacaacagac ttgctggtca cttggctcta
     3781 taaattaacc gtcacagctg ctgactacaa tttagcttct gttatcggta tcttaatctt
     3841 tacagtttca gctatcttta gcttacttgc ttatacaagg acagcatcct acaaggaagg
     3901 agcggctaaa taatgaaaaa caaacgtcga tttcaacttg gacttgttta tgctacttta
     3961 atcatcctct caatcatttg gctcttccca atcgcttggg ttattcttac tagcttccgt
     4021 agcgaaggaa ctgcttacgt caattatttt attcctaaga cttttacatt aaatcactac
     4081 attaatcttt ttactaatga aacctttcct tttggaaaat ggtttatgaa tactcttatt
     4141 gtagccactt ttacatgtat tatttcaacc ttcataactg ttgccatagc ctactctctt
     4201 agccgaatta aatttaagtt tcgcaatggc tttttaaaac tagcccttat tttaaatatg
     4261 ttccctggtt tcatgagcat gattgccatc tactatatct taaaggcctt aggtttgacg
     4321 caaaccctaa cagcacttgt ccttgtttat tcctcaggtg cagcccttgg tttttacatt
     4381 gcaaaaggat tttttgatac cattccttat tcacttgatg aatcagctat gattgatggc
     4441 gctactcgta tggatatttt ctttaaaata acccttcctt tagcaaaacc aatcattgtc
     4501 tatactgccc ttcttgcttt tatggggcct tggattgact ttatctttgc acaagttatt
     4561 ttaggtgatg ctacaagtaa atataccgtt gctattggac tattttcaat gcttcagcca
     4621 gataccatta acaactggtt tatggccttc actgctggtt ctgtcttaat tgccgtccca
     4681 atcactctcc tcttcatgtt tatgcaaaaa tactatgttg aaggcatcac aggtggttct
     4741 gttaaataag ttacaacata ccaaaccttc taagggcaag ccctttagga ggtttttgat
     4801 aaataaggcc gtagataatc atattagtcc tcacatttat cttcagatca gccaaatgta
     4861 ttaatgattc cttgactttt accatgaatt cggatataac aaaatgatga catctaaaat
     4921 taccattaaa gatattgcac gagaagccgg ggtttctgtg gcgaccgtat cttacgtgtt
     4981 aaaccagcgc aatgatcaaa aaatcagcga agaaacccgc aataaagtgc tacatattag
     5041 taaccttctt aactatacac ctaatcaggc agccaggtca ttagtcacac aacaatcaaa
     5101 cagtgttgtc ctgtattaca aatatgcaga tagcatttta gctagggctg aacaactgca
     5161 taccattagc tttctatccc gcctattgca tgagcaggac tatcagctcg tttgccttaa
     5221 tgaagatagt attggaaaat ttgactgggc tgaccttatt ctgaccttag atattgaaaa
     5281 tgataccttt tacaaaatcg ggaaacttaa tttcaagccc ttaattgcta ttgatagctt
     5341 tgttaatgac cctctcttct tccaaattaa cacagattac gcaaacctaa aagaccaagc
     5401 agatcagttt tttaaggatg aaccttatac cctgctgact ctccctatca acaatcaaga
     5461 acggtatgag gctatctcca ccctttttcc aaacctacat gtggttgacc acatgaggga
     5521 gttgcctagc tttgaaaaga aaaatctttt actcattgat gagagtttgg accaattctt
     5581 tgtgaactct tcgcaaaaac tctactgccc acgtttttcc ccagaaaaag ggcaagttat
     5641 cttagaggcc atcgagtggg ccaaacaacg acaactcaac aaaaatcata atcttctcgt
     5701 ctaatagcac acaaaacccc ttgcacttca actgaagtgc aaggggtttt gatatgacat
     5761 cggtgtcaga gttctcttag gatggtaacg aatctctaaa ctgtgttttg tagaacaccc
     5821 aggtcagcat taataaggta ccaaatactt ggcaattcat aatcaatacg ggatcctgaa
     5881 taaagaaact aagagcaatt gccaatagac ttggccaggc caaacaattg accatcaaac
     5941 caagaccttc tgaaaaattt cgaatcataa acagccgtga tcgtttggtc aaggtcaaaa
     6001 agaaagcgcc tagaccaatc acaataaggg tgcccacata caccatgaga acaaacgtca
     6061 acagaagaaa accaagtaca ctggctttat tactgtcata ccattgctga ttgacaaagt
     6121 ccttcactgc tttaactgtt gtgaggtcgg cgagcgactg ctgctggcct ctaacgtgag
     6181 cacggagttc cttgccttca ggggtaacaa aacgccattc tttttttgtc acaataattt
     6241 gcctggttga ttctgatgcc aatttctcta aatcgacctt tgtcggtaag acagccaaaa
     6301 ccaattcacc atctcgtcga atcagagatt gaccctgaaa ggtattttcc tcaacctttc
     6361 cttgggtcaa tgcgtgatag gttttgtctg tgataggcga caaactctta gttacaatcc
     6421 gttccaaggg atacgttgtc atgttagcat agtgtaacgt aactggaatc attattaagg
     6481 cgttcaaaaa aacaataata agaaggtttt gccaccaagt aaactgagct cggctggcaa
     6541 acattgccct aggagaaagg caatgtgaaa tgtaagaaat aggaaattgt tttttatctg
     6601 gcattaaaca ttactccttg tcaggttacc ctttggtacc accacttgtt aaacctgaga
     6661 cgaaattttt ctgtaggaag aaaaagagaa ctgaaatagg cactgcaatc agaatagctc
     6721 ccgcagcaaa taaggttacc ttaggatttc tagcatcatt cgtaataaac gattgaagac
     6781 caactgctac cgtataattt tcttgcgccc gtaaaaggaa tttagccaac atgaagtctc
     6841 caaaaggacc cataaaagcc cataaggatt gtactgcaat cattggtcgc acaagtggaa
     6901 ggacaatttg gtagaaggtc ctaaagtgtc cagaaccatc taacttagca gattcgtcca
     6961 agtcataagg taccgtgtca aaataaccct tcatcaacca ggcattcatt ggaataccac
     7021 caccgacgta aatcaaaatc aagaaccagt attggtttaa ggcattaaag agccaagcca
     7081 taacaaagta agccgttaaa gccgccatgg taggaaccat ctgtacgact aggaaaaaga
     7141 ttaaactttt tttgcgtcca aagaaattgt aacggctata tgcataacct gtcaaagtga
     7201 tgactgttac ttgtatgagc atggtgaaga atgccacgat taaggtattc caataccatc
     7261 ttaagtataa ggtatcttga aagagtgttt taaagttaga caaggtccat ggcccatcaa
     7321 aatgaaaact aaaggctgtg gtatttcctg gtctaaaagc tgaactgacc gtcactagta
     7381 tcgggaataa aatcacgatc gctaaggaga ttagatagag ataggtcaat aattgggtga
     7441 agaaacgttt gcgtttcatg gatgttgaaa cataggtctt tctcattatc tctcctccat
     7501 atcaaaagct ttaaatttct taaaggtaat caaggatacc gaaatcacta ttacagagat
     7561 gattaaggtt actgccgacg ccatcgaaaa ctgaggtgag gtttgtgtgg tcaacttgta
     7621 aatccaggaa atgaggatat cagttgtccc agctccgccc ccaacagtac caggcccccc
     7681 attattaaac agataaataa tagagaaatt attgaagtta aaagtgtact gtgcaatcag
     7741 cgtcggtgct gcaaccgcaa aaatcattgg catggtgata tgacggaatt tctgcaagac
     7801 tgtcacacca tcaacggttg ctgcttcgta taaatcagct ggaattgcct gtaaaatacc
     7861 tgataccaaa atatagatat agggaaatcc tagccaacct tgcaccataa taacagccac
     7921 ttttgtccag aaggggtctg tcttccagga cacaattccg aagtcaacga aaggaaggaa
     7981 tttctcgatg aaaggaagaa cctgaacatt catggcccca atggaatcat taaagaaatt
     8041 accaaaactc ataatagaga taaatgctgg aacagcccat ggtaacaaga atatcacacc
     8101 aaagagacgt tttcctctga taaaggattg attagcaata acagccgttg caattccaat
     8161 aacaatttga agacttgtcg ctgctaatgt ccagatcagc gtccatgaca aaacagatgt
     8221 aaatgctgtt ttgaaggtgc ttaattctaa gatattaaga aaattcttca accctaccca
     8281 atccaataaa cgatacggag gaatatgcct aaaatcgtaa ttggtaaaag caatcagcaa
     8341 ggtcactaaa actggaaaaa taatagcgaa agccattgct aaataagccg gaatgatgag
     8401 caagtaagga aatccttttt cataaatcgc ttcaagggtc tctttagccg tcaccggaac
     8461 cttcttgcct tcattcattt gatgtgcaac taatttagca tctctcatag caaccaaatg
     8521 aaaaatacca aataagacta gagtaattaa ttgcatggcc cccttaatta acataaataa
     8581 ggagtgatct tgcattggaa catttccaag actgtaaaga cctgttaaag caggaattcc
     8641 tagaactaca atttctagta gctctaggaa gaaaacaact aataagactc cccctttaac
     8701 gatttgttta ttgtatagtt gtcccaaacc tgggataaca gatagccaca tggctttatt
     8761 aggcttctgt tgtctttggt tatctaccat tttttattcc tcttctcata aatgatttaa
     8821 ggttatcctc cttatcatac taacttcctt ttaagctgac taatttattt ttttatagta
     8881 atggtttgtg cttcatgacc tgtctttata ctataagtct caggacttgt ccataaaaca
     8941 ttcccaatac tatctttttt aacaagtttg actgctattt ctttattgat tggtagatga
     9001 gtatcaaaga accagtttgg gtacttggca atggtctgag tgttattaaa tagaggacca
     9061 acagcattct tagcgtcatt tgcccccatc tcaaaaacat cacccatgag atatagttgt
     9121 tcccctggta ctgtcttaaa atcattgatg agtaaacgaa caggaatttg tttatcagtc
     9181 aatacttcaa aggcttggta actattgctg gtttgcttat cggcagttgt cactgaaatg
     9241 ttataataat ttgctggaac cgtcggtact tttaaggtga taagggtatc tgaccaagaa
     9301 aggatctcag cagagatttc tccaaaagtc acttgacctt tagaattacc aaaaccttga
     9361 cctgaaatag taatcttatt tccagcaata cccactgaag catcgacatc tccaagttgt
     9421 ggtgtcttgt cctctccttc ataagtccat acagcgactt gccccgctcc aagttcaaag
     9481 ctactaatag tgccatctgc tccaactatt aattcttgac cgccaagaag accttctaat
     9541 ttgtccttgt agctattttg gggcaaggct gttttagcat tagtaattgt ataaccattc
     9601 gtttgatctc tattaatagc cactagtgcg acatgattac caaatttacg ctcaaatacc
     9661 aaaacatgat cactaatcca acgttgttca gttgttccat aagctaaagc ttgattttgt
     9721 tttcttaaag gagctagctt actaatcact ttataggctt gtgactcttt attaaaactt
     9781 ggcatatcac cacgattatt aggatcttta tcacctgttg catactgctc tgtaccataa
     9841 taaatattag gcacacctct agatgttaaa agcaaagcat aggcttgatt aacagcagtt
     9901 tgattattag ccacttttgt tgcgaagcgt tccatatcat gattatcaat aaaggtcacc
     9961 tggtctgtca cttcattagt cacctgatct ctattttcaa gaaccttgta aaaatctcgc
    10021 atggtaaacg ttgaaaaacc tgtatacaac tgtctaatag cattggcaaa tctaaaatct
    10081 aaaagcccca tcccactatt gttagcaaat gtcgtcatat cataatcatc gtcggtatgt
    10141 cccgagaacc attccccgaa aacaaagaca ttatgttttt catagatatg actcaaccaa
    10201 tttttttgcc aaccttgtga catatgttta acagcgtcaa ctcggatacc atcaacaccc
    10261 aggtctaacc atttatcaat agcttctttc atgtactggt caactttcgg attaatgtta
    10321 tttaaatcag ctagtccgta cattgaatga taaatagaat tttcataagt actaaaatcg
    10381 gtccaagatt catgattaaa aatcttgtct ttatcatctg aaaatttacc aactaattta
    10441 ccgtttttat ataaagcgcc atcttctttg aaagttgtgc cttctttttc ggctgtagac
    10501 gtatgattag gagcaaaatc aataactacc ttaatatggt gttgatgagc tactttgact
    10561 agttgttgaa agtctgcttc agtgccaaaa tgctggtttg ttttaaagaa gtccttagcc
    10621 caatacccat gatatgcagc acttccatta gaaggatcaa tactgtcgat attttcaaca
    10681 ggagaagaaa tccaaatggc agaaatcccc atatctgtca ggtaaccatc cttaatcttg
    10741 gcgatgattc cttgccaatc acctccatgg tattttttaa ggtcattttt atcaaaaaca
    10801 tcagtcttac cattattaga ggtattacca tcgttaaaac gatctgttac aatctgataa
    10861 atcgtatctg ttgaaaaatc tgctttgttg gtgacagaag cattatcggc agcagaaaca
    10921 gttagtggaa atgaaattaa gccaagtaaa acagcacttt tcgttaataa cttataagtt
    10981 ttgatatgta attctctcat cgctttttcc ttttcaaata accttttagt tactaaacac
    11041 aatatatcca tcctggagta attgtttttt gtttttagtt accccattgc ttgtcaaaat
    11101 ctgtcctcct tccttaatag aataagcact ctcaccattg ttaagaacaa gtgaaatgct
    11161 ttgctcacct aagctgcgtt tcagaatgag gcagtcttcg tcataatcaa tgctaatagc
    11221 tccttcttga agagctggct gttggcgaag agcaataatc gcttttactt tgctaaagaa
    11281 atccttgtcc cgctcagcat catcccaagg aaaaccgcga cgcgaatcag ggtcgtaacc
    11341 cccttccatt gccacttctg tcccataata caagcaagga atgcccataa agataaagag
    11401 caatgctaaa gcagctaaca atttatcttt ggaaccctta acttgagtga agaaacgatg
    11461 cgtatcatga gaatccaaaa gattcaagtt catgcgattg acttgccaag tgtttcgcat
    11521 caaaatgttg cttaatttct cagccaattg ttttgatgtc atggtttcaa aagcaaagta
    11581 atccaaacag gctttggtga aagcataatt cattatccca tcatactgat cccctgccaa
    11641 ataaggatag gcatcatgcc agttctcccc aattaaaatg gcatctgctt tttccgcttt
    11701 aaccgcctga cggaaacgtc gccaaaaatc atgagatact tcatcagaaa catctaggcg
    11761 ccaaccatcg atacaaaatt ctttaatcca gtatcgccca atctcaatca gatagtcttg
    11821 cacgtctcta tttgaggtat tccatttggg catatagtta catccagcaa aggtttcata
    11881 gtttactagg tccatggatg ggtgttcatc atgagtcata aaccaatcaa aaaacttaga
    11941 ctctttaccg tatctcaaga catcctgaaa ttccacggca tcactgctgg catggttaaa
    12001 gacagcgtct agaataatct tgattcccat ttggtgagct aaatcaatca attcttgcaa
    12061 atcatattta gttccaaatt gtggatcaat agcgtagtaa tcactaatat catatttgtg
    12121 attactaatg gattgaaaaa ttggcgttaa atagatgaca gtaatcccta aatctttgag
    12181 gtaatctaat ttttctgtaa ttccctttaa atctccacca gcaaaacttt taggagtcgg
    12241 tttttctaac caacccatgt tgacataact gtcatctttt tcccaatccc cacggttaaa
    12301 gcgatcaaca aagatttgat aaaaaaccgc gtcttttgtc caaggaatga cttgatggac
    12361 atcaacagca ttgatataag gcatttggaa gaaattataa aacccatttg aaaaatcgta
    12421 actagtagtt aacccatctt cactaaaata ataggtttga ccttcttctt caattttaaa
    12481 aatgtaggcc aacctcacat cgtctaatgt taaagtagtt tcataataag catgcgtatg
    12541 gtctgtatag gcaactgaca tttccaattc ttcttggaat tcatgataac catatttagg
    12601 cccgtggacc aaattaacag taatacctct atcttctttg gccacgcgta atcggatgac
    12661 aagttccttt tgagatatgg caaagcaata cctcgaatca ggaatatgga gcattccggc
    12721 aacattcatt tctacctcca ttaataaagt attgacgatt acttcttttt ctttaaaacg
    12781 aaaaaatagc agagcgactc atacttctat atttttagta tctgtttcat cttcatcaaa
    12841 ctaacagaaa tagaagctgg gcgtacttct ttatcccagc ctcccttctt tgccttattc
    12901 agcgtatttt tgttcaatag cttccttaat agtttttgct gcttcttttg cagctttaga
    12961 tgcttcttcc ttaccagaag ccacgttaaa gaacatattt gcacctggtt cccaaacttc
    13021 agccatttct ggaatatttg gcattggttg tgcgttctca aactgactaa tcacagcttt
    13081 cgttaactca ttaccctctt tagaagcata gttacgtgct gttaagttag ctggaatttc
    13141 ttgtgtttta tcatagaaac gtttttggtt ttcagcattg gtcacataat caaggaattt
    13201 ttgagcagtc gtttttcctt tagaatagtt tgaaataacc caagctttac cacctgcgaa
    13261 cggttggtat tttttaccat ttgtcaaagt aggaatttcc attaccccaa agttaacacc
    13321 agcatcttta aaggagctag ctgcccatgg accatcaata atgacaccag cttttttaga
    13381 aataaattgt tcagtgataa aatcaccagc ttttttagtg tcttgcattc cttgaggcca
    13441 agtgccatac cactgtttcg cgtagtttaa cccttcaaca gttccgtcgt tacctaggcc
    13501 taaatcactt ggtttggttc ctttatcacc aaagatataa ccgccataac cggcaattaa
    13561 accataacca taatagaagt ctgtccattt cgccaagaaa cctacagatt ttcctggttc
    13621 agaggcaaaa gcaaacttag aatctttttg taagacttct aattcagtaa agcttttagg
    13681 tgcttgaggc actaaatcct tattataata agttaccaaa gtttcaatta cgtctggtgc
    13741 accataagtt ttaccatcaa tggttactaa tttcttaacc gtatcatcaa actctttgct
    13801 gtttcctaaa gtaacctcag cgatttgacc ttcagttcct aagccaccta cacggtcaaa
    13861 aggtgctaaa aagacatctg gtgaagctcc tgttggacca tccgttgata gcttatcaag
    13921 agtatccatc ataccttctt ttttgacttt aacggtgact ttatgttctt tttcaaactc
    13981 acccttaata ctcttaatgt aatcaacata tccctcatca acagagacta caatagtctt
    14041 tccgtctgtt tccttaacgc ctttaccacc accacttacg caagcagtta ggggtagcac
    14101 tgaagctaag gataaagttc caagcacaga tgtttttttc caattccatg acataacatg
    14161 ttcctcccga aatggttttt tcttaaacac aaatcaaatc atctatctac tctcatcata
    14221 ttagcaaata aaagataaca taaggcattt aaaattaata acagtaatag tttcgaaact
    14281 taaacgttta agttaagttt atcgtaatcg ctttcatttg tcaataactt ttttactttt
    14341 cttttctttt cttttaagag catatcagta gttttcctgc taattgaaaa caaggcattt
    14401 actgtcacac aagtctgtcc tttctttttt tctacaactt tatttcgtca gcgtaaataa
    14461 aacaaaagct ctataatctc ttgaatgggt aaatccactg tagagattat agagcttttt
    14521 gaatatcaaa aaagtcccat aagatttata atgaaaagca accaaactct cattagaaag
    14581 actcttatgg aacatatcaa tcatacctta caacacatcg gaattaaaga caaaaatatc
    14641 actctaaaca atgttttgaa ggatgaatct cctactgagc tcgttactac ttatcagaaa
    14701 ggtagatagg aaatcctgat gaagagtgtg tccaagggat tatcaattag gtcttagatt
    14761 acaaaatagt aattcaggta tgctctactg ttttcaacac tagttctgca atttgttttc
    14821 gagcttgctt agcttctctt attggaaaaa taacatacgc atgaatcatt ttttctgcaa
    14881 taatcagctg attagcaatt cgttgtcttg ttaattgatc atgtaaagac ataatgtctg
    14941 ggtaaaaaat ctcttttgtc ccaacaaagg tcgttatttc aggcaaatcc tcataatcac
    15001 catacaaagg actgacataa ggaagttgca tattaccttg accacctgcc catagttcac
    15061 ctatatcacg aagacgacga gcatctaggt aagcttcttc tgcttcaaaa tccttgtagg
    15121 cttgattttt aaaactaaca tctaaccaag gagatataag aattaattgc tttggctggg
    15181 gtgctgtttt agtatctcgt atatacttag caaacgccaa agccataccc ccaccagaag
    15241 aatcaccaat gacactaact tgactcggtc cattagacaa gcaaaattgc tggtaagctt
    15301 caactaattt aggatagaca tcttgataag tagcatacgg tactttagga aagttaggaa
    15361 aaacaacctt ggcattagtc agctgtataa gatgcttaac cgtcttaaaa tgaagttttt
    15421 cgggtcgtcg taaatagaca ctgccataaa gataataaat aacaggctga tgactttgct
    15481 tttgatcatt aataataaaa acttgcatcc cgttcacatc gtattcttga gttgaaaaac
    15541 ctactatctc ttcaggatta tctagatgat aagggatatt acgattacgc tgcgtctcaa
    15601 cccccttata attatcaact tttttagata acttatactt tctataagca aacattttct
    15661 caaccaacca acttgaaaag gagcgctttt gaccatattc aactatcaaa atacctagta
    15721 tcactccaaa aaaaatcacc acagcaataa tgaccactta ttatcctcgt tttctaatat
    15781 tattttacta ttgcaagtct ttcctatttg cccaaatttt actataaaaa taagggttca
    15841 ttttatagtt gggcactggc tgcttcgttt ctagaaatgg ttgcactttc ttatcaaaag
    15901 ctaaccagcc attccaaccg agatggatgg tatcttgcat aaagtaggac tcaccaccat
    15961 cttttgagaa gtcagcaatg cgatgaaatc cttgtgactt taactggaat tttattttac
    16021 gaactgccgc ttgatactta tcttgattta agccggtata atccgcccaa gctttattaa
    16081 caggagttat aacaaagagt acatctgttt ttcgtttagc aaattctgat aataaaagct
    16141 gaaaatcatt gtattctggt gacgccaggt aactataatt aacttggaaa ttcttataaa
    16201 gattgtattt aggtgctatt cgtttacgat aaaatgtatt tttaatccca aaacggttgt
    16261 tggttgttgc taattggcct ctcttagttg ctaatgcatt caattgttta tacgaaaata
    16321 ctttaggtaa tcccttaacg cgaggcaaaa ttcttttttc atagttagta gatttgccta
    16381 aaaaactaaa aagggactct tctctcaatg ctacttgatg ttgacatttc aaaatagctc
    16441 tgtctaaccg actaagagac ttacccttac ttactttttt caataagttt gatttagaca
    16501 caccagggtt aagctcaagc aaacgctttg ctgcaaactg tgattcttta tcagttctag
    16561 cttttagtaa aaattcaatc acttgagtgt tagacaagta catctgaacc gcactaggat
    16621 taatcccttg agcagtaaac cattgaggag atactacaaa gatggctttt ttcttttgca
    16681 tttcattggt aatttgttgc ataccataat aatgcgacaa agatgctgat cctctcttac
    16741 caattaaaaa tggtctatag ctccgcttgt agcgctctgc aagcaccgaa gggtgcatac
    16801 tatccattcg actccattcg ctagaaccaa aaaaaggcac aaaacgacaa gtctcatctg
    16861 ataaagcttg tcttttaatc aaaccatttt taaaagaact atctgtgatc gcaacggcat
    16921 ttgctttttc ctgagctatg gaatgatcaa gttgtgtggg aaaactaaaa atagtaatca
    16981 ctactaaaac aaaggcaata agaagaggac ctagaattaa ccagagtctc ttaagcatgt
    17041 tgcaattcct ctacaccttg aacaatctta gttactgtgt tccaatcatc acgaccaaat
    17101 tctgaaatag gtactttaat attgaacgtt gactctaact caacaatcaa ttcgactgtc
    17161 ccaagactat ccaacacacc tgcatcaaac aagtcttcgt ccatcatctc tgaaacatct
    17221 tccataaaca aacggtcaaa caattcaatt acagtttctt caatagacat ttttatactt
    17281 ccttttatta atgtagtggt ctgttaaacc aaaggtcatt taaaaaacca gagaataaca
    17341 gaagtgaaaa catgacaacg tggaaagtta ccacgataca taagacatgg aatgccctgc
    17401 tttgaaagag gggtgataag ccttttttct tacggtgacg gttgatttct tttttcttac
    17461 gaatccaagc atcattaata ataagtccta taccatggaa aagcccataa gcgatataat
    17521 accaggttaa gccatgccaa aatcccatga ctagcatatt aaccagatag gcaacacctg
    17581 aagtgacatt tcggttttta aaggttttat gcttaatcaa tagatgaacc aatcgcataa
    17641 acacataatc tctaaaccag aaggacaaac tcatgtgcca tctattccaa aaatctttca
    17701 aacttgcaga taagaaaggc atgttaaagt tttctggtgt tcgaataccc aataggtagg
    17761 aaataccaat cgcaaacatc gaataaccag caaagtcaaa aaagagattc aagccataca
    17821 cgtacataac cagaatagtt tccttattaa agtagccacc aaccataaaa gccttatttt
    17881 caaggagagg aagtaagatg ccacccaaac aataggaaat gatatgcttg tatagaaaac
    17941 ctaacatgag atacatgact gccttattga gcattgcaaa ataatcatca cgttcaggaa
    18001 gtttatgata atcttcctga aaacgtctga agcgatcaat ggggccactt gagaaagtgg
    18061 ggaagaaaat catgaagcga ataaaatccg gtaaacgaac ctcttggaga gtcccgtcac
    18121 gcatctcaat aatcattcca atacttttaa aggtcaaata tgaaatccct agaaaactaa
    18181 agagtgtttg agaagaagga cctgttaggg gagcaacttt gacccagata agggggaata
    18241 aggccatcgc aatagctaag taaaacactc ctgcactatt agctttctca cggtatttct
    18301 ggtaagcaaa aacactgatg atttgccaca aaagataaaa gagaaaagca actaattgtt
    18361 gataatgatc tcctccaaac ataaagagaa taaataccaa actcaccaag gtttcataag
    18421 tgcttaagcg cttttgttta aaaatcccaa tgatgactgg taagaaagcc aaaataagat
    18481 agacaaagta aatggggtta ccgtaaggtt ccatgtaggg aatgtggcta aaaaacatca
    18541 tcatcggtta ttgacctcat taatcaaagt tttaatatct atcttaccat ttggagttaa
    18601 tggtagacta tctcgataaa gaaacttaga aggcatcata taactcatca tatgatcctt
    18661 aacagaggct ttgatagctt tggttaactc caattcccta tcaaaacgct ctttaacccc
    18721 atctttgaca acgatatagg ccaagagatt ttgaacctta tgttctttat tgtaccttgg
    18781 aacagccaca gccgatgcta ccattggcga ttgattcaac tgttgggaaa catcttctag
    18841 ttcaatacga taaccagcat atttaatctg aaaatccaaa cgcccaccat acagcaaaat
    18901 attatcttct gtcaatgagc caatgtcccc tgtatgataa gcaggctgtc ctttaaaagt
    18961 aaagaaagct tctgcggttt tctcaggatt gttgaggtaa ccttttgaca cagcaggccc
    19021 tgtaacaata atttcaccct gctcccctga agataactcc ttaccatctt catcaataat
    19081 ataggtcgga gaatctggtt tggggtagcc aatcggcaag cgggtataat tatccaccat
    19141 ttctctggta atttcaatag cggataaggc tacagtcgct tctgttggtc cataggcatt
    19201 gataatcttg gcactcggga aacgttcaaa gagttttcga gccgtagaaa cggttaattc
    19261 ttcgccatca aaataaaaat gcgttaaagc tggcatctta gcctggcaaa aatcatcgct
    19321 aagcatggcc atatcagcaa atgaaggagt cgatgtccaa atgccaactg gaagttgcgc
    19381 aatcgttgta aataactgtt taaagtctgc caccaattct tttggaagtg caaataaggt
    19441 tccacctaaa gccaaggtag gtgcccagta catgacagaa aggtcaaagg aatagggtgg
    19501 ctgcgccagc atttgtggct gtttaggtac atcaaatgct gcatcttcga tcatccagtt
    19561 ggtaaagctc aaaagattgt catgtgaaat ttgcacccct tttggttgac ccgttgttcc
    19621 agaggtgaaa ataatgtaat aattatcatc tcctttaaca gaatgtgttc gctcataggg
    19681 catttctgct aattttgctg actcaatctc tgatagtgag acaagggaaa tcccttcaat
    19741 agtaagaggg aattcctcaa tagcaatgat taaactgggc tttgcaatct caataatcgc
    19801 taaaatacgt tctggtgctg aatgcacatc tactgggata taggcatgac cagatttggt
    19861 taaagctaca aaagtagcta acatatcata agtttgcgcc ccaaaaacca aaactggaga
    19921 ttttgccagt aaagctaagc tatctataaa tgcagcaatg ctatcagaat ctcttttgag
    19981 ttgtccgtaa gtgaggcgtt cccctaaaca atcataaact ggaaaatcag cctgtgtctg
    20041 agcaaactgc tcaatactgt caatcatatc cttaatcatc agtcaatctc ctaaaattca
    20101 ttataaataa agttactttg accatgtcct agataaccaa agaaatagac cagcaacatt
    20161 aaaatcagat aaaataaaat tgtcttgcca ataaaaacag caattcctct cattcttgat
    20221 ttgtttttta tcatacttta ccttcttgta ttagtgaaat aatgtcctaa aaaattctac
    20281 tcgaaaaaac ctaacttgac aagctaaaag tcatattatt aaaaa
//
LOCUS       DATURV010000028        19239 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DATURV010000028 DATURV010000000
VERSION     DATURV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19239
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.028"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..684
                     /locus_tag="VCF75_000976"
     CDS             <1..684
                     /locus_tag="VCF75_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP2546551.1"
                     /translation="RGETGDKGEQGPAGKDGDRGPVGPAGKDGQDGKDGLPGKDGKDG
                     QDGKDGLPGKDGKDGKDGQDGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGK
                     DGKDGQNGKDGLPGKDGKDGQPGKPAPKTPEVPQKPDTAPHTPKTPQIPGQSKDVTPA
                     PQNPSNRGLNKPQTQGGNQLAKTPAAHDTHRQLPATGETTNPFFTAAAVAIMTTAGVV
                     AVAKRQENN"
     gene            884..1321
                     /gene="msrB"
                     /locus_tag="VCF75_000977"
     CDS             884..1321
                     /gene="msrB"
                     /locus_tag="VCF75_000977"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP2546552.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDNFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYDQMEKEGYAQWLTLFDET"
     gene            1451..2470
                     /locus_tag="VCF75_000978"
     CDS             1451..2470
                     /locus_tag="VCF75_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEP2546553.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWCLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKTKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDALHHSNTLNGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSLGVS
                     ADSFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKMGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            2677..3102
                     /locus_tag="VCF75_000979"
     CDS             2677..3102
                     /locus_tag="VCF75_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2546554.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            3121..3612
                     /locus_tag="VCF75_000980"
     CDS             3121..3612
                     /locus_tag="VCF75_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP2546555.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRATDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            3629..4438
                     /locus_tag="VCF75_000981"
     CDS             3629..4438
                     /locus_tag="VCF75_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP2546556.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            4435..5262
                     /locus_tag="VCF75_000982"
     CDS             4435..5262
                     /locus_tag="VCF75_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP2546557.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            5398..7047
                     /locus_tag="VCF75_000983"
     CDS             5398..7047
                     /locus_tag="VCF75_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2546558.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQDLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            7051..7839
                     /locus_tag="VCF75_000984"
     CDS             7051..7839
                     /locus_tag="VCF75_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2546559.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            7833..8879
                     /locus_tag="VCF75_000985"
     CDS             7833..8879
                     /locus_tag="VCF75_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP2546560.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            8994..10391
                     /locus_tag="VCF75_000986"
     CDS             8994..10391
                     /locus_tag="VCF75_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEP2546561.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQGLADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVNLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            10493..12289
                     /gene="uvrC"
                     /locus_tag="VCF75_000987"
     CDS             10493..12289
                     /gene="uvrC"
                     /locus_tag="VCF75_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEP2546562.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            12474..13076
                     /locus_tag="VCF75_000988"
     CDS             12474..13076
                     /locus_tag="VCF75_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEP2546563.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            13201..14610
                     /gene="pepV"
                     /locus_tag="VCF75_000989"
     CDS             13201..14610
                     /gene="pepV"
                     /locus_tag="VCF75_000989"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEP2546564.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDQLADDKHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFAHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLNVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGTLSMNA
                     GVFTFDNQSADNTIALNFRYPKGTDAATLKAGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(14678..16054)
                     /gene="mnmE"
                     /locus_tag="VCF75_000990"
     CDS             complement(14678..16054)
                     /gene="mnmE"
                     /locus_tag="VCF75_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEP2546565.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPADLIPISVLTNHNIDLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    16225..16360
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            16390..16890
                     /locus_tag="VCF75_000991"
     CDS             16390..16890
                     /locus_tag="VCF75_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEP2546566.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            16955..17320
                     /gene="rplL"
                     /locus_tag="VCF75_000992"
     CDS             16955..17320
                     /gene="rplL"
                     /locus_tag="VCF75_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEP2546567.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
     gene            17619..18218
                     /locus_tag="VCF75_000993"
                     /pseudo
     CDS             17619..18218
                     /locus_tag="VCF75_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            18398..18723
                     /locus_tag="VCF75_000994"
                     /pseudo
     CDS             18398..18723
                     /locus_tag="VCF75_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            18714..18991
                     /locus_tag="VCF75_000995"
                     /pseudo
     CDS             18714..18991
                     /locus_tag="VCF75_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            19037..>19239
                     /locus_tag="VCF75_000996"
     CDS             19037..>19239
                     /locus_tag="VCF75_000996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HEP2546568.1"
                     /translation="MTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLEFLLN
                     RANSLEELLLKAKEVNLTIDLKQ"
BASE COUNT         5993 a         3466 c         4038 g         5742 t
ORIGIN      
        1 cgcggcgaaa ctggtgacaa aggagaacaa ggtcccgctg gaaaagatgg tgaccgtggt
       61 ccagtaggtc cagctggtaa ggacggccaa gacggtaaag atggtctccc aggtaaagac
      121 ggtaaggacg gccaagatgg caaagatggt ctcccaggta aagacggtaa agacggtaag
      181 gacggccaag acggtaaaga tggtctccca ggtaaagacg gcaaggacgg ccaaaatggt
      241 aaagatggtc ttccaggtaa agacggtaag gacggccaag acggtaaaga tggtctccca
      301 ggtaaagacg gcaaggacgg ccaaaatggt aaagatggtc ttccaggtaa agacggtaag
      361 gacggtcaac caggtaaacc ggctcctaaa acaccagagg tccctcaaaa accagatacc
      421 gcaccacata ctccaaaaac ccctcagatc cctggccaat caaaagacgt gacacctgct
      481 cctcaaaacc cttctaatag aggtctaaac aaaccacaaa cacaaggtgg taatcagctc
      541 gcaaaaacac cagcagctca cgacacacac agacaattgc cagcaacagg cgaaacaacc
      601 aatccattct ttacagcagc tgctgtagct atcatgacaa cggctggagt tgtagctgtt
      661 gcaaaacgtc aagaaaacaa ctaatcctct ttttggctac ctaactgatg gaaagtaaaa
      721 ctcagagaga acctcaaggt tctctctttt ttgaatcaag tttttgaatc aagatagtag
      781 ctctaggtat aacgtcttgt ctgaggtgct gtgaacaaat cagttaccac tcacctcttt
      841 ttttggtaaa atgtaagcac taaaacatca aaaggagttt tccatggaaa catcagatga
      901 gttaaaacaa cgcattggtg atctttctta cgaggtcacc cagcatgcag caacagaatc
      961 tccctttaca ggagaatatg ataatttttt tgagaagggt atttatgtcg atatcgtcag
     1021 tggtgaggtc ttgttttcat cactggataa atttaattct ggctgtggtt ggcctgcctt
     1081 ttcaaaaccc attgaaaatc gtatggtcac aaaccacgat gactcttctt atgggatgag
     1141 acgagttgag gttaaaagcc gtgaagcagg atcgcatttg gggcatgtct ttagtgacgg
     1201 ccccaaagaa gctggaggct tgcgttactg tatcaattca gcagctctta aattcattcc
     1261 ttatgaccag atggaaaaag aaggctatgc ccaatggctc acattgtttg atgagacata
     1321 acagggtttc aaaatgacta aaaaagaagc gtcagtgatt cccacaagca ctggattggt
     1381 gaggcgtacg ggtcaatttt acaagccttt gtaaaaggtc tataataggg aatgaaaaaa
     1441 ggaggttagt atgtcaactc acttaagaaa acttccaggg ctgttacttt gcttattgtt
     1501 agctcttcca gcctggtgtt tagggcgctt gtttcctatt attggagcac ctgtttttgc
     1561 tattctttta ggaatgttgt tagccttgtt ttatgaacat cgtgacaaga ctaaagaggg
     1621 aattagtttt acatccaagt atattttaca aacagcagtg gttttgcttg gttttggatt
     1681 aaacctaacc caagttatgg cagtaggcat gcagtcttta ccgattatca tttcaactat
     1741 tgcgacagct cttttggtag cttatggctc acagaaatgg ctgcgcttag atgtcaatac
     1801 agccaccttg gtgggtgtag ggtcttccat ttgtgggggg tctgctattg cagcgacagc
     1861 tcctgtcatt aaggcaaagg atgacgaggt tgctaaggca atttcagtca tttttctctt
     1921 taatatgtta gcagctttgc tatttccaag tttaggacaa ttactaggct tatctaatga
     1981 aggttttgct atttttgctg ggacagctgt taacgacacc tcttccgtga ctgcaacggc
     2041 gacggcctgg gatgcccttc accattccaa tacactaaat ggcgcaacca ttgtcaaatt
     2101 gactcgcacc ttggctattc ttccaattac tttaggttta tccctttacc gagcgaaaaa
     2161 agagcacgac atcgttacag aagaaaactt tagccttagg aagtcattcc ctcgtttcat
     2221 cctcttcttt ttattagctt ccctcatcac gacattgatg accagtttgg gagtttctgc
     2281 cgatagtttc cattccctaa aaaccttatc aaaattcttt atcgtgatgg ctatggcagc
     2341 tatcggttta aatacaaacc tggttaaact gattaagatg ggcggtcagg ctatcctttt
     2401 aggagctatt tgctgggtag ctatcaccct tgtcagttta gccatgcaat taagtttggg
     2461 catttggtaa tcagacacga gaaatcagtt tgactgattt ctttttttgt atcttatttg
     2521 tgtagaattg tctacatttt aaagagaata atgcatttta aaaacgtttt catttgataa
     2581 aataaaccta tcgttaaacc agttcagtaa ggacaactgg ttgagataag caattaccgt
     2641 ttactcagcg gtttttgtta atagaggagg ataggaatga aacgaaaatt tctcattggg
     2701 agtcacggca gactagccag tggtttgcaa agctctattg acattttagc aggtatggga
     2761 caggcacttg aaaccattga tgcttatgtt gatgatagtg attatactag ccagattgat
     2821 gactttatcg ctggggtagc agcagatgag cagggcctta tttttactga tttgttaggc
     2881 ggcagtgtga atcagaaaat ggtaaccgct gtaatgaaca gtggcaaaga caatatcttt
     2941 ttaatcacaa acagtaactt agccacttta ttgtcgttgg tatttttaaa accaggcgaa
     3001 gcgcttacta aagatgaaat tgtaaccgtt atcaatgaat cgcaagttca gttagtggat
     3061 ttggtccctg agacaaattc agaagatgac ttttttgatt aatatctagg aggaagacaa
     3121 atgattactc aaattcgtgt tgacgaccgt ttgattcacg ggcaagtggc agttgtatgg
     3181 accaaagagt taaatgcgcc acttttagta gtggctaatg atgaagctgc aaaaaatgaa
     3241 atcacacaaa tgacgcttaa aatggctgtg ccaaacggca tgaaactgct tattcgttca
     3301 gttgaagact cgattaagct ctttaatgat ccacgcgcaa ctgacaagcg tatttttgtg
     3361 attgtcaatt ctgttaaaga tgcttgtgcc attgctaaag aagttccaga cttagaagct
     3421 gttaatgtgg ctaatgtggg gcgctttgat aagtcagatc cagcaagcaa ggttaaggtg
     3481 acgccaagtc ttttattaaa cccagaagaa atggcagccg caaaggaatt agtcagttta
     3541 ccagaacttg atgtctttaa ccaagtctta ccatcaaata ccaaggtcca tttgagccaa
     3601 ttagtcaact aaagagaggt aaaaaagtat gttagtacca gcaacaatgg cagctttagc
     3661 tgtattgatt tgttttgggg gaaattattt aaccggtcaa agcatgatgg aaagacccct
     3721 agtagttgga ctggtcacag gtcttctttt aggagatatg aaggtcggta tcttaatggg
     3781 ggcctctcta gaagctcttt tcctaggaaa tgtgaatatt gggggtgtta ttgcagctga
     3841 gcctgtaaca gcgactgcca tggcaacaac ctttaccatt atttcacata ttgaccaaaa
     3901 agcagccatg accctagctg ttccaattgg aatgttagca gcctttgtgg ttatgttttt
     3961 gaaaaacgtc ttcatgaata tctttgctcc aatggttgat aaagctgcag cagctaacca
     4021 tcaaggtaaa ctagtgatgc tccactatgg cacttggatt atctattact taatcattgc
     4081 ctctatttct ttcattggta ttttagtagg tagtggacct gttaatgcct ttgtggaaca
     4141 cattcctcaa aatctcatga atggcttgag tgctgcggga ggccttcttc cagctgtcgg
     4201 ttttgccatg ctgatgaaat tattgtggac caacaaatta gctgtttttt acctattggg
     4261 ctttgtctta acagcttatt tgaaattacc agcggttgca gttgctgccc taggagcagt
     4321 tatttgcgtc attagttccc aacgtgatct agaattagac gcaattacta gaggtgcaat
     4381 tagtaaacaa acaacctttg actctaaaga atcagaagag gaggacttct tcgcatgaca
     4441 tcacaagata atttgactaa agaagaccgt aaaatgcttc gctcagtctt ttggcgctca
     4501 tggacgatga atgctagtcg tactggggca acacaatacc acgcagtagg ggttatttac
     4561 actttactac cagtcatcaa tcgtttttat aagactgata aggataaagc agaggcactg
     4621 gtccgtcata caacatggtt caatgccacc atgcatatca ataactttat catgggtctt
     4681 gtggcatcga tggaaaagaa aaacagtgaa gatcctgatt ttgatgctag tgctattacg
     4741 gcggtaaaag catctctaat gggacctatt tccggcgttg gagattcctt cttctgggga
     4801 attttaaggg ttattgctgc aggaattggg atttccttgg caagtgcagg ctcagccatg
     4861 ggagctgttg tcttcttact cttgtacaat attcctgctt ttattattca ctactatagc
     4921 ctttatggtg gttattctgt aggtgcagga tttatcaaaa aactttacga atctggtggt
     4981 ataaaaatcg tcactaaaac ctcaagcatg cttgggttaa tgatggtagg ctcaatgact
     5041 gcttcaaatg taaaatttaa aacgattctt acagttgctg cgaaaggtgc caaagaagca
     5101 gcttctattc aagattacct tgaccaattg tttattggta ttgtaccatt aatggtaaca
     5161 ctagcagctt tttggcttct acgaaagaaa gtgaatatca actggattat gtttggtatc
     5221 atgttccttg gcattatcct tggactttta ggtatttgct aataatctcc tagcaataaa
     5281 ggtaagtaag acaagttttc ttgttttgct tacctttttc cttttaagat gatgagactt
     5341 gttttgatga aaaacgtagt agcattaaga aatttgttat aatgaaagga gcttaagatg
     5401 cgaggtgaac aggtggaaga acattttaaa aaacaattac aagatgatat ttctagacat
     5461 ttttcgtacc aatcgttaat gttatcattg ttgctaattg gtctttttat tattttttca
     5521 ttagcgccac aacaattagg cctctatcgt gatatcaatg ccactgcaac ccgttatcac
     5581 cgtttgatta gcaaacaaga agccttgctt gatgacttag ggaaaaatag cttactacct
     5641 tttttaaata aaaacctcag cactgctgat ttaagcaagc actattttca tttgcgccat
     5701 agcagccaaa cgtcaccaga acttttacta ttttcaccta gtcaagacct tttatttgct
     5761 agtaacccac atttaggaaa tgtttttagt aaatctgttt atattcaaga agtcttgaga
     5821 gcagctcatc ctccaaaaac cttgtttaaa gttgctatgg atagtgaaga tggtcactac
     5881 ttgatgatca ttaagccaat gattgatcaa aaccaactaa agggatatgc ttttttagtg
     5941 atgagtggca aagattttct tcatcctaca aaaacattga cgtcagagtt ggtcattgct
     6001 gataagctgg acaatacgtt tacgttttcc aatcgtgagt ttatagcatc tagtctagat
     6061 aagatcaata gccagtattt acaccattat tttgtttttc aagataatcg agcctttatc
     6121 acaagaaagg ttgccttaca aggaggtctt tggctttaca tgtataggcc cttgattcca
     6181 atggtatcgg tgatgttatt ttcacttatt tcatcagctg ttatctttgt gatcttgcaa
     6241 cgtaaatcaa gtggtttagc taaccgaatt gcagctaaaa attcaagagc aatcaatcaa
     6301 atggttagag atatgagtgc gatctctcgg caagaaaaaa gacgtattga ccttgagagt
     6361 caagatgaat ttcaatattt atctgatcaa atcaatcaaa tggtagagcg attgcaacag
     6421 ttacatgata aaacgttaga tttggaaact caaaaattat tatttgaaaa acggatgtta
     6481 gaagctcaat tcaatccgca tttcctctac aatacgctag aaaccatttt gattacgagc
     6541 cattatgatt ctgccctaac agaaaaaatt gttatccaat tgacaaaatt gttgcggtat
     6601 agtctcacgg attctagcaa acctgtcttg ctcaaagatg atttaagtgt catcgagtct
     6661 tatttagtga ttaatcaggt gcggtttgaa gaattgcaat atagcattaa cctatcccca
     6721 gaccttgata gtcttgcagt gcctaaattg ttcttgttac ctttgataga aaatgccatc
     6781 aaatatggct tgaaagaacg acacgatgtc aagattaaca ttgcttgtta ttatcaagat
     6841 gaccacatta ttttttctgt gcgagataat ggttcgggaa ttgatgctca tcaccaaaaa
     6901 gtgattcgag agcaattgga agctggagag tcacaccatg ggttaattaa ctcttatcgt
     6961 cggcttaagt atcatttttc agaagtatcc ttggtttttg atcaaggtga taaacagttt
     7021 aatgttagtt accatgttaa ggagtagtct atgtacaaat tagtcattat tgaagatgaa
     7081 catattatta ggaaatggtt gcgatacgct attgattata aagctttaga tattttggtc
     7141 attggcgagg ctaaagatgg caaagaagga gctgttttaa tcaaagagag tcaaccagat
     7201 atcgttttaa ccgatattaa catgcccatc atgacagctt ttgacatgtt tgaagcaaca
     7261 aaagatcaaa cttacgccaa aattatttta tctggctatg ccgattttcc aaatgcaagg
     7321 tctgccatac actacggtgt acttgaattt ttaacaaaac cgattgaaaa agcagctctt
     7381 tgggaatgtc ttcaaaccat tattgctaaa atagaaaagc aaaaggggtc taatcaaaaa
     7441 acagatgctt gcgtctatat tcccttaccc cagatgactg atcagattcc tgaagtagtc
     7501 aaagatattt tggagtgggt acatgctcat tttcaagaca agatcagcac gagccgccta
     7561 gctcatgact tagggtatag tgagagttat atctatcaaa atatcaaaaa gcatcttcag
     7621 atgcctttaa gtgattacat taatcaatat cggattaatc aggcaattca attgatgcaa
     7681 caagaaccag atttaatggt atatgaaata gcacaagctg taggtattta tgactatcgc
     7741 tattttgata gggtttttaa aaagtatcta ggacaaactg taaaggcttt taaagaagag
     7801 cattttatga aagataccga taggagtaag ccatgttaaa actcaaacgc aaatggctat
     7861 taagcttttt gttagtaata attattctag caatcgttaa cattgcaatg tatatattct
     7921 catcgtcaaa aaaagatagt gctaaagaat tagttatctt gactcctaat agccaaacta
     7981 ttttaacagg gactattcca gcctttgagg aaaagtatgg ggttaaagta agattaatcc
     8041 aaggtgggac gggccaactt attgatcaat taggtcgaaa agataagcca ttaaacgctg
     8101 atattttctt tggtggcaat tacactcaat ttgaaagcca taaagattta tttgaatcct
     8161 atgtttctcc gcaggtttct actgtcattt cagattacca attgcctagt catcgtgcaa
     8221 ccccatatac gatcaatggc agtgtactga ttgttaataa cgaattagca agaggacttc
     8281 atattaccag ttatgaggat ttgctacaac cagctttaaa aggcaaaatt gcttttgctg
     8341 acccaaatag ctcatctagc gctttttcac aattaacaaa catcttactg gctaaaggag
     8401 gctatacgag ctcagaagct tgggagtatg tgaaacgttt attactgtct atgaattcta
     8461 tcagagcaac aagttcttca gatgtttacg aatctgttgc agaaggtaaa atgattgttg
     8521 gtttgaccta tgaagaccct tgcatcaatc tgcaaaaaag cggtgcaaac gtttcgattg
     8581 tttatcctga tgaggggaca gtatttgttc cgtcatcagt cgctattatc aaaaaagcaa
     8641 aggcaatgaa agaagctaag caatttatta attttatttt atcatcagat gtacaaaatg
     8701 cctttgggca gtcaactagt aatagaccta tccgacgaga tgctcaaacg agtcatgaaa
     8761 tgaaaccatt agatgaaatt attactttaa aagaagatta cggatatgtt actagacata
     8821 agaaaaaaat attgaatacc tataatcgac tacatgaagc attagaaaga ggaaagtaga
     8881 ctctaggctt gttgttgcta tactagtagt gttacaaaat agcgtagtca ttttttgaaa
     8941 tcgatttcat aatctggtag actaaggtta tcacgaaaag gaagctatat gtgatggctt
     9001 atcaaactat ttacccttat acaaatgaag tccttcatac atttgataat atgaccgatc
     9061 aaggtttggc agatgtcctt gaaagagctc accttctcta taaaaaatgg cgcaaggaag
     9121 atcatttaga agaacgtaaa gcccagcttc atcaagtggc taatattctt cgtcgtgacc
     9181 gcgataaata cgctgaaatc atgaccaaag acatgggaaa actgttcaca gaagcccaag
     9241 gtgaagtaaa tctttgtgcc gatattgctg attactacgc tgataaggca gatgaatttt
     9301 tgatgtctac gccactagaa acggattcag gtcaagctta ctatttgaaa caatcaactg
     9361 gtgttatcct agctgttgag ccttggaatt ttccttacta tcaaatcatg cgggtctttg
     9421 cgccaaactt tattgttgga aacccaatgg ttttaaaaca cgcgtccatt tgcccacgct
     9481 ctgcccaatc tttcgaagaa ttagttcttg aagctggtgc tgaagcaggt agtattacca
     9541 atttgtttat ttcttatgac caagtatcac aagtgattgc tgataaacgt gtggttggtg
     9601 tctgcttgac aggttccgaa cgtggaggag ctagcattgc agaagaagct ggtaaaaact
     9661 tgaaaaaaac aacccttgaa ttgggtggcg atgatgcctt tattatcctt gatgacgcgg
     9721 attgggatca attagagaag gtgctttact tctctcgtct ctacaatgca ggacaagtat
     9781 gtacctcatc gaaacgtttt atcgtgcttg acaaagacta tgaccgtttc aaagagttat
     9841 taacaaaagt cttcaaaaca gctaaatggg gtgacccaat ggatcccgaa acgacgttgg
     9901 cgccattatc atcagctcaa gctaaagcag atgttcttga ccaaattaag ttggccttgg
     9961 atcatggtgc ggagttggtt tatggtggtg aagcgattga tcatccaggt cattttgtca
    10021 tgccaaccat tattgctggc ctcacaaaag acaaccctat ttactatcaa gaaatctttg
    10081 gcccagtcgg tgaaatctac aaagtgtctt ctgaagaaga agcgattgaa gtggcgaatg
    10141 attctaacta tggccttgga ggtactatct ttagtagcaa tcaagaacat gcgaaagccg
    10201 tagctgctaa aattgaaact ggtatgtctt ttatcaactc aggctggaca agtcttccgg
    10261 aactcccatt tggtgggatt aaacattcag gctatggccg tgaattgagt gagcttggct
    10321 ttacatcttt tgtgaatgaa caccttatct atattccgaa taaaacgaat aatagcaata
    10381 cgaaagtttg atctagattg ttaaaggaga tctgctcgcc ttaataaaca atcaaggcgt
    10441 tacactcttc ttttttaaag tcaaaatttt aaaaatttga taaactagta acatgaacga
    10501 actgattaaa cataaactag aactcttacc agacagccct ggctgttact tacataaaga
    10561 taaagaagga actattatct atgtcggtaa ggccaagaat ttgaaaaaac gcgtccgatc
    10621 ctattttcgt gggagccatg ataccaaaac agagttatta gtatctgaga ttgtagattt
    10681 cgaatatatt gtaacagagt cagataccga agcgcttttg ttggaaatta acttgattca
    10741 aaaaaatatg cctaagtaca acatcaagtt aaaagatgat aaatcttatc cctttctcaa
    10801 aatcacaaat gagtcttttc ctcggttagt gattacaaga tatattaaga aaaatgatgg
    10861 attgtacttt gggccctacc cagattctta cactgctaat gaagttaaaa agttattaga
    10921 taggattttt ccatttaaaa agtgtaaaaa tcctatcaat aaggtctgtt tttattacca
    10981 tttaggccag tgctgtgctc ataccatttg tcatacagac aaggcctatt gggatcgttt
    11041 aattgatgat gttaagcact ttttaaatgg taaagatgac aaaattattg aagatcttcg
    11101 ttctaagatg ttagcagcgt ctgaagaaat ggcatttgaa cgtgccgctg aatatcgaga
    11161 tttgatttca ggtattgcca ccatgcgcac caagcagcgg gtgatgagca aggatttgca
    11221 agatagagat atctttggct attatgttga taagggatgg atgtgtgtcc aagttttctt
    11281 tgtgcgtcaa gggaaattaa ttcagcgtga tgttaacctt tttccctatt ataacgatgc
    11341 agaagaagat ttcttaactt atatggggca attttaccaa gataaacagc atttcatccc
    11401 taaagaagtc tttattccag aagctattga tgaagaactg gtagccgcta ttgtgccaac
    11461 caaaatcatc aaacccaaac gcggtgaaaa aaaacaactg gtagctttgg ctactaagaa
    11521 tgctcgtgtt agtctgcaac aaaaatttga tttattagaa aaagatatca aaaaaacgag
    11581 tggtgctatc gaaaatttag gtcagttact taggattgac aagcctgtgc gcattgaagc
    11641 ctttgataat tctaacattc aagggacaag tccagttgcg gctatggttg tttttgttga
    11701 tggcaaacct agtaagaaag attatcgtaa atttaagatt aaaacagtgg tgggaccaga
    11761 tgattatgcc agtatgagag aagtgctttt tcgaaggtac agtcgggtga aaaaggaggg
    11821 actgcaagct cctaacttga ttattgttga tggaggcgtt ggacaagtca atgtggccaa
    11881 agatgtgata gaaaaacaac ttggactcac tattcctgtt gcgggacttc aaaaaaacga
    11941 taagcaccag acccatgatt tgctttttgg aaacccactt gaagttgtac cgttgccgcg
    12001 ccgttctgaa gagtttttct tactgcatcg gattcaagac gaagtgcatc gctttgcagt
    12061 tacatttcat agacaagtgc gacgtaaaaa ttctttttca tcaacattag atcatatttc
    12121 agggcttggc ccaaagcgaa aacagttatt actaagacac tttaaaacca taacagcgat
    12181 tgcatcagct acctctgagg agattcaagc tttaggtata cctaaaacag tagttgaagc
    12241 aatacagcaa cagataacag acaataaaaa tgatagatcg tctccttaaa taagtgttat
    12301 gttttcagta ctgatgaggt aaaaaggcgc tacttgttac gcacgacatt tcagttcgtt
    12361 attttgaaac tttcagaaag ttgcgaagtg tagtgagttt atgattttaa aaactatttt
    12421 atggtaaaat aaaatggtat taaaaaaaac agatttagat aggaagtttt gaaatgaaat
    12481 ttctagagtt aaacaaaaaa cgccatgcca ttaagacctt taatgaccag ccagttgact
    12541 acgaggattt gcgaacagcc attgagattg caaccttagc acctagtgcc aataacattc
    12601 aaccttggaa atttgtagtc gttcaagaga aaaaagctga gttagcaaaa gggttacctc
    12661 tagcaaataa agtgcaggtt gaacaggctc aatatgtagt ggcgctattt tcagacactg
    12721 atttagcttt acgttctcgt aaaattgccc gtatcggtgt caaatcatta ccagacgact
    12781 taattggtta ttatatggag actttgccac cacgttttgc tgcttttaat gaggttcaaa
    12841 ccggtgagta cttagctatt aatgcaggca ttgtggctat gaacttggtg ttatccttga
    12901 cggatcaaaa gattgcttcc aatattatcc ttggttttga taagtcaacg accaatgaga
    12961 ttttagacat cgatccgcgt tttcgtccag aattattgat tactgttggc tatagtgacg
    13021 agaaaccaga accaagttat cgtttgcctg ttgatgaagt catcgaaaga agatagagtc
    13081 aggacaatta gttaaatgta atgtccagat gcgctactga gcgaccacta ttgttagact
    13141 gatactattt tggcttattt taagttaaac attatcccta aaacgaataa ggagagtata
    13201 atgacaacca ttgattttaa agcagaagtt gacaaacgta aagaagctat gctagcagat
    13261 ttagttgacc tattacgcat taattcagaa cgcgatgacc aattagctga tgataaacac
    13321 ccatttggcc caggtccagt taaggctttg gagcatttcc tcgcaatggc tgagcgtgat
    13381 ggctacaaaa cgcgtaatat tgataactat gcaggtgact ttgaatttgg tcaaggtgat
    13441 gaagtattag gaatctttgc tcaccttgat gttgtcccag ctggtagcgg ttgggataca
    13501 gacccttatg agccagtgat taaagacgat cgcatctatg cgcgtgggtc ttcggatgat
    13561 aaggggccaa ccatggcgtg ctactacgct ctgaaaatca ttaaagaatt gggtcttcct
    13621 gtgtctaaaa aagttcgttt cattgttggt accgatgaag aatctggctg gggggatatg
    13681 gattattact ttgcccataa tggcttgaaa aatccagatt ttggtttctc accagatgct
    13741 gaatttccaa ttatcaatgg tgagaaagga aatatcactg aatacctcca tttcgctggt
    13801 gacaacaagg gcgcttttgt gcttcatcgc ttccaaggtg ggttgcgtga aaacatggta
    13861 ccagaatctg ccacagctgt cattacggca ccgcatgacc ttaatgtgct agaagcagcc
    13921 ttggagcaat ttttatcaga acatggtgtt aaaggttcta tgaaggctac tgatggtaaa
    13981 attgaagtaa ctattattgg taagtctgct cacgggtcga ctccagaagc gggtgttaat
    14041 ggtgccacct tgctggctaa attcctgaat caatttacct ttgaaggggc tgctaaggat
    14101 tatttacatg tggcaggtga ggtgcttcat gaagactttg cggcagaaaa actaggcctt
    14161 gcttataccg atgaccgtat gggaaccttg agcatgaatg cgggtgtctt cacctttgat
    14221 aatcaatcag ctgacaatac catcgccttg aatttccgtt atccaaaagg gactgatgcg
    14281 gcaaccttaa aagcaggatt ggaaaaatta ccaggattaa ctaaggtaag tctttctgag
    14341 catgaacata cacctcatta tgtgccaatg gatgatgaat tagtagctac cttacttgct
    14401 gtttacgaaa aacaaacagg tcttaaaggc tacgaacaag ttatcggcgg aggaaccttt
    14461 ggtcgtttgc tagaacgtgg tgtggccttt ggagcgatgt tcccaggtga tgaaaacact
    14521 atgcaccaag ctaatgaata tatgccttta gaaaatattt atcgctcagc agctatttat
    14581 gctgaagcaa tctacgaatt aattaaataa gatgcttatg acagcaaaaa gagctggaac
    14641 actgttccag ctcttttgtg atgattagag actgttgtta tttaccaaga caaaattggc
    14701 taaagagttg ggtaatgagc tcatcaggag cagcatcccc tgtgatttct ccgagaattt
    14761 cccaagttct ggttaaatca acctgtagta aatccactgg catgccaagg gctaaaccat
    14821 cattgacagc ttccaaactt tggactgcct tttcaatcaa agaaatgtgg cgggcatttg
    14881 acaggtaagt ggcatcttgc tctaccaaac cagcgttgtc aaagaaaagc tggttgatgc
    14941 gatcttcgat caggtcaata ttatggttgg ttaacaccga aataggaata aggtccgctg
    15001 gcaactgttc caattcaatc ttttgttcca aatctgtttt attcagcaat ataatgcggt
    15061 tactgtcttg actgagattt aacaaagccc ggtcttggtc ggtcagtttt tcagaagcat
    15121 tcagcacaag aagaacaagg tcagcttctt gaagagcttt tttagaccgt tcaactccga
    15181 tttgttcgac cagatcatct gtctcacgaa taccggccgt atcgacaagt tttagaggaa
    15241 ctcccttgat attaacgtat tcttcaatga catctcgggt ggttcctgca atatcagtca
    15301 caatagcctt gtcttctcgc aacaaattat taagtaagct tgacttaccg acgtttggac
    15361 gaccaataat agctgttgat aagccctcac gcagaatttt accgcgttta gctgttcgaa
    15421 ggagattttc caaaaggctt tggaattctt gtgttttctc acgtagcaga gcagtggtca
    15481 tttcttccac atcgtcgtat tcagggtagt caatattgac ttcgacttga gctaaggtgt
    15541 tgagaatttc ctgacgggtg tcattgataa gctgggaaag ggagccatcc agttgcttga
    15601 ctgcaatcgt catagccttg tctgttttgg cacgaataat gtccatgaca gcctcagctt
    15661 gagtcaaatc aacacgacca tttaagaagg cacgtttggt gaattcgcca ggctctgcca
    15721 ttctagcgcc ttggcgaatc aatagttgca agatttcatt tgtcacagca ataccaccgt
    15781 gcgtattgat ttcaaccaca ttttcacggg taaaggtttt gggggctagc atgactgaca
    15841 ccataacttc atcgataata gttcctgtct taggatcaat gatatggcca tagttaatag
    15901 tgtgtgaggc gacttgctct aagttctttc ccttaaaaac agattgtgca atggcaagcg
    15961 catctgtccc tgataaacga acaatgccaa tagctccttc accaagaggg gttgaaatgg
    16021 cggtaattgt atcaaattct ttagttatac tcatgccatc tattttaaat caagtcctag
    16081 tagaaagcaa ggtattattt tgcatagaaa ataggactag caccattttg agtttagcta
    16141 atactaaaaa aaataaatgt aaaaaaatca tttttttaat aatttttatt gacaatagta
    16201 ttataaaaga atataatgat ataggttttg ataaaaaact gacctaagac agcaggggag
    16261 catgctcata atattcctgc cgaggcacaa aacgtgatta aagaaaataa cgtatttgta
    16321 ctttcgtgtc taggtttagg tgcgattttt tttgtaccta gcccaaaaat aaaaacggag
    16381 gtaaaactaa tgagtgaagc aattattgct aaaaaagctg aacaagtcga acttattgct
    16441 gagaaaatga aagcagcagc aagtatcgtt gttgttgatt cacgtggtct tacagttgat
    16501 caagatactg ttcttcgtcg ttcacttcgt gaaagtggcg ttgagttcaa agttatcaaa
    16561 aactcaattt tgactcgtgc agctgaaaaa gcaggtcttg atgagttgaa agatgtattc
    16621 gtaggaccat ctgcagtagc attttctaac gaagatgtta tcgcaccagc taaagttatc
    16681 aacgacttta ctaaaactgc tgacgcactt gaaatcaaag gtggtgcaat cgaaggcgct
    16741 gtttcttcaa aagaagaaat ccaagcactt gcaacattgc caaaccgcga aggaatgctt
    16801 tctatgcttc tttctgtact tcaagctcca gtccgcaacg ttgcatacgc tgtcaaagct
    16861 gttgcagaaa acaaagaagg cgctgcttaa gtcgtctcag agtagcttaa ggctacgctt
    16921 aacattttaa attaattatt tggaggaaat cacaatggca ttgaacattg aaaacattat
    16981 tgctgaaatt aaagaagctt caatccttga gcttaacgat cttgtaaaag ctatcgaaga
    17041 agaatttggt gtaactgcag ctgctcctgt agctgctgca gctgctggtg gtgctgaaga
    17101 agctgctaaa gattcattcg acgttgaatt gacatctgct ggcgacaaaa aagttggcgt
    17161 tatcaaagct gttcgtgaaa tcacaggtct tggtcttaaa gaagctaaag gtcttgttga
    17221 tggagcacct gctaacgtta aagaaggcgt tgctgctgca gaagctgaag aaatcaaagc
    17281 taaacttgaa gaagctggag caacaatcac tcttaaataa gaagcagata gcaatttgag
    17341 agcgaaatcc cgatttatca gtcttttaga gtttatagcg atttgaagaa actgataaat
    17401 tgatttggtt tcttgccaaa aatcctgccc aaaaaatctt tggcaggatt tttattttct
    17461 ataaaaatta agctgagtag agtggttatt ctatctatat gtgtctagta ataatttttt
    17521 tatgtgaacg agaacatttt aataagtgtt ctcatttttt tattttcagg aggaaaatca
    17581 tgcaaatgaa tgaacagtca tcacgacaaa tggtgattgc catctatctc atttattcca
    17641 actcaaaatg atagtactcc ttggctgttc caagtcgaaa ttgttagcga ttaagaaaac
    17701 ctacgagact atggtttgat cgatgaagtc caacagtctc ccagtgaaaa agggcgtttg
    17761 gctaataaga tttatttagg aaagttggaa catgattcca ccccagtctt aagttcagac
    17821 ggggccagtc tcaaaaacag accagccccg tgttaaattc agcccctagt gagactgaaa
    17881 agagtgaaac tgaaggtagt gaaactaaag agagtaattt agtcattgag gacgaggagg
    17941 aaagaaagga gtgcactagt gtgaaaaaga ctgagggtca ctttacccgt caagttgatc
    18001 agatcaccaa atatgacaaa gattatattt ggagtttggt tcattcccaa ttacgtgaag
    18061 gtggtttatc acaagctgct agtgacttag tcatgagcta ttttgaagaa cgctatgctt
    18121 atgcgctgga acacatacgg ttcgcaagaa cagctgaagc tattgcggaa tatgtcttta
    18181 atggtgtctt atccgaatgg accaagcaac taaggtgaca agaagcgaag tgaggtgagt
    18241 aagatgtttt ggtggctatt attaggtgtt tggggactaa ggacaatttg gttgattatt
    18301 gaagttatca ctgcaccaga aatggaagat tatagttgag taaggacgtt acataagcag
    18361 taaggtcctt ttattttgtc agaaaggaat gagagatatg aaatttttag atttgtttgc
    18421 aggaattgga ggctttcgat tgggtttaat taatcagtgc catgagtgta ttggcttttg
    18481 tgagattgat aagtttgcga ggcaatctta caaagccatc tatgagaccg aaggagaaat
    18541 agaatttcat gacattagac aagtcacaga ccaagacttt agacaactta gagggcaagt
    18601 ggatatcatc tgcggtggtt tttcccttgt caagcatttt cactcgcagg cagacgactg
    18661 ggatttgagg gcactaggag aactctcttc tttgagatta ctcgagcgac caaacagata
    18721 aaaatcatac ccataatcac atccaatcag cgccattgac cgtaattcag ataaaaaatt
    18781 gatatggaat tatgccttgg aacgaaatct tcgtatgatc tcagaccgta tttctaaaat
    18841 agctggggcg aaaattattg aaaagcattt ctcttaccgt gactatcaaa aatataaagc
    18901 gactagccat aagtttgaac taaagcaacg tctttatttt ttgatgcagc agtcaaagtt
    18961 ctttgatgat tttttagaaa aagcagagta attacatgtt catattgatt ttagtcagaa
    19021 gcatagcaga ttcatgatga cggatcgagt aatgaaaaaa ccaattcgag gccgtcaact
    19081 cagtaaacga gatttatatg atgaagaatt ttttcgtaca cattttgcca agatagaaat
    19141 tgaaagtcga ttagaatttt tgttgaaccg tgccaattca ttagaggagc tactattaaa
    19201 agcaaaagaa gtgaatctaa ccattgactt aaaacaaaa
//
LOCUS       DATURV010000029        17594 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DATURV010000029 DATURV010000000
VERSION     DATURV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17594
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.029"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            436..1785
                     /locus_tag="VCF75_000997"
     CDS             436..1785
                     /locus_tag="VCF75_000997"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEP2546569.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
     gene            complement(2134..3642)
                     /locus_tag="VCF75_000998"
     CDS             complement(2134..3642)
                     /locus_tag="VCF75_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2546570.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIANFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
     gene            4330..5001
                     /locus_tag="VCF75_000999"
     CDS             4330..5001
                     /locus_tag="VCF75_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEP2546571.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGVQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSSRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
     gene            complement(5100..5999)
                     /gene="galU"
                     /locus_tag="VCF75_001000"
     CDS             complement(5100..5999)
                     /gene="galU"
                     /locus_tag="VCF75_001000"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEP2546572.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(6032..7048)
                     /locus_tag="VCF75_001001"
     CDS             complement(6032..7048)
                     /locus_tag="VCF75_001001"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP2546573.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     RNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            7346..7795
                     /locus_tag="VCF75_001002"
     CDS             7346..7795
                     /locus_tag="VCF75_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP2546574.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            7788..9494
                     /locus_tag="VCF75_001003"
     CDS             7788..9494
                     /locus_tag="VCF75_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546575.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPFLFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLVQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            9497..11281
                     /locus_tag="VCF75_001004"
     CDS             9497..11281
                     /locus_tag="VCF75_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546576.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTMTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAVVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            11399..12166
                     /locus_tag="VCF75_001005"
     CDS             11399..12166
                     /locus_tag="VCF75_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEP2546577.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            12276..12722
                     /locus_tag="VCF75_001006"
     CDS             12276..12722
                     /locus_tag="VCF75_001006"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEP2546578.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            12803..14164
                     /gene="radA"
                     /locus_tag="VCF75_001007"
     CDS             12803..14164
                     /gene="radA"
                     /locus_tag="VCF75_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEP2546579.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            14353..14850
                     /locus_tag="VCF75_001008"
     CDS             14353..14850
                     /locus_tag="VCF75_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEP2546580.1"
                     /translation="MSYFEHFMAANQVYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVRKDMAKLRASSLIPDDVVINGAVYDVDTGKM
                     TQVME"
     gene            14981..15691
                     /locus_tag="VCF75_001009"
     CDS             14981..15691
                     /locus_tag="VCF75_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEP2546581.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            15873..17318
                     /locus_tag="VCF75_001010"
     CDS             15873..17318
                     /locus_tag="VCF75_001010"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEP2546582.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NEAGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
BASE COUNT         5344 a         3162 c         3757 g         5331 t
ORIGIN      
        1 ttttaagtat tcaaatgatt gccattttta gataatgaga gtaagtaatg ttaagaagat
       61 agcatgagaa agtatttaca ggtgattggg gcttcaattg cctttgtggg tgtggcttta
      121 tgcattgatc atatgcatga tggggttatt acccgtgttg aaatgataaa atccttaaaa
      181 taagttatta cggtaaagcg gcctcagggc cttttttcat gatattttta ggaaatatga
      241 aatcctattt tctcttaaaa aaaacactat gatgagaata tgcttgcatt gacacatgtt
      301 ttatgtctga aaaaacgaaa accttttcaa aaaacctagc tttttcacag gtgaaaacca
      361 ctaaaaacca ttgaaatatt agatagaaaa ggttatcata ctaatgaaac ttaaaatttt
      421 aagggagcga aactcatgtc acatattaca tttgattatt caaaagtcct cgagtcattt
      481 gcgggacaac atgaaattga ttttttacaa ggccaagtca cagaagctga caagttattg
      541 cgtgaaggaa ctggaccagg ttcagatttc ttgggttggc ttgatcttcc agaaaactat
      601 gacaaagagg aatttgcacg tatcttgact gctgctgaaa agattaaggc agacagcgaa
      661 gttcttgtgg tgatcggtat cggtgggtca taccttggtg ccaaagcagc gattgatttt
      721 ttaaatcatc attttgctaa cttgcaaact gccaaagagc gcaaagcccc acaaattctt
      781 tacgctggaa actctatttc atcaacttac ttagctgacc ttgtagagta cgttcaagac
      841 aaagaattct cagtaaacgt gatctctaaa tcaggtacaa ccactgagcc agctattgct
      901 tttcgcgtgt tcaaagaact tttggttaaa aaatatggcc aagaagaagc taacaaacgt
      961 atctacgcta ccacagacaa agttaagggt gctgttaagg tagaagctga cgctaacaac
     1021 tgggaaacat ttgtggtgcc agataatgtt ggtggtcgtt tctcagtctt gactgctgtt
     1081 ggtttgcttc caattgctgc atcaggtgca gatattactg ctcttatgga aggggcaaat
     1141 gctgctcgta aagacctttc ttcagacaaa atctctgaaa acattgctta ccaatacgct
     1201 gctgttcgta atgtgcttta ccgtaaaggc tacatcactg aaatcttggc taactacgag
     1261 ccatctcttc agtactttgg cgaatggtgg aaacaattag ctggtgaatc agaaggaaaa
     1321 gatcaaaaag ggatttaccc aacttcagct aacttctcaa cagaccttca ctcacttggt
     1381 caatttatcc aagaaggtta ccgtaacctc tttgaaacag ttattcgtgt tgataaccct
     1441 cgtaaaaatg ttatcattcc tgaattagca gaagacctag atggtcttgg ttaccttcaa
     1501 ggtaaagacg ttgattttgt taataagaaa gccacagatg gtgtgcttct tgcccacaca
     1561 gatggtggcg tgccaaacat gtttgtcact cttccagctc aagacgagtt tacccttggt
     1621 tacactattt acttctttga gcttgccatc gctgtttcag gctacatgaa tgctgttaac
     1681 ccatttgatc aaccaggtgt tgaagcttac aaacgtaata tgtttgcgct tcttggaaaa
     1741 ccaggttttg aagcattatc agcagagctt aatgctcgcc tctaatcaac agatagtcaa
     1801 aaagacttct tccttgtaca agagcttaaa agttagccaa gcaatctaag aattggggcg
     1861 gcacttccat catcaagtgg atacaaggtt aagtaaaagc gataataaag tgcgcttcca
     1921 gaacatggga gcgcacttta ttatgccaaa tttcagataa aaccttcaaa aaagggaaga
     1981 agatcctaca agaggagttg aaccgctaaa acagttccag ttctgtttct aagcaaatag
     2041 caatggttaa aaagagagct tatcaaggtt gcaagtgaca accttaaaga cccccctaga
     2101 ctgtgaaagt atctaggagg gactgtcaaa aaattaagaa ggaactctcc aaagttcttc
     2161 taaacgtttt tggtagtggt tctggtgcaa ggctaagatg gttttgatta aatcttcgcg
     2221 ctgctgttgg atacagtcca aactcaaacc aaacaagcgg gtctctttgg gaaaaacacc
     2281 gagttctttt aagaaaggca agagcttctg actggtcaca accagatcta caggtttatc
     2341 atagggataa atattatcag ataagatatt gacccgtgac agttcaactt tgtaagaagg
     2401 gatagttgag gtgatgaaat tagctataac gttaccaaca gtttcttggc tctcaataac
     2461 agtaatattg attggagcaa tagagctttc caagatctgc tcaaggtata aacacatcaa
     2521 gtgtaagtga ccagaactaa tactgccttg acggcctgtg tcataaagcc aatctttgat
     2581 aacggtttca agcttatcta gtagtagcat gttgccttga tattgatcaa aataataacc
     2641 atcaaaggta attaaagttt gtagattgta gagagttctt ttgaaaaaag gaattaagtt
     2701 agggatatgc tcatgaatat cggaatactt tccaactagt gcctgaagac gatggattag
     2761 taaatgataa tcgggatctt tggtgattaa atggtaaagt tgttgtcgct gatgacttgt
     2821 ccagtaccca ctcaagaagg agttatcagt cgttaaataa attaaaaaga gatagtcaaa
     2881 atcatcagac gaaaatgtga cacgagttcg aggtgctagc tcatccacgg caatggtttt
     2941 gatattgtca aaaataggca aggatttgag atgttcaaaa agagctaaat gaggcaaatg
     3001 aacaggatag cgatggcgct tccaagtcaa catcaacagc acatcaaaaa acaagaagcg
     3061 tctatctaaa aaggcagaag gttgtaagtt tttttgtgct gcaaagataa aagcatgaat
     3121 cgtttcaatg tctgcaggtt gtatatcata taatataatg ccgtactctt tatgtaaaag
     3181 agcaatcaaa taccgtattt taagttcatc tccgataaca gtattgtcta caatatttaa
     3241 accaatgtct tgtaaataat gttttaattt atgaataaga cgataagctt ttgactggga
     3301 tatcccttgt tttcgtgtat aaacagcgat tgacttgtgt ttgggattat ctagtaataa
     3361 gaatgtcaac atttttagag tatctgataa ggcaaaaata tcataaaaaa atgcttctcg
     3421 cttatcattt aatacctcac aataaatacg agatttaata agctcaatac gtaagtaccc
     3481 tttaaataag gcgttaaatt gtcgaagata ctgctttatt tttaaagcag tcaaagttgt
     3541 ttgagaacaa gctgctgcca tcgtcagttc tttttctgtc aacaaaatag ccagtaggat
     3601 tttctggtca ataatatcgg attccaaata ataatctaac aatgtaattt ttctctcctt
     3661 aaattaaatt gtataatcct atatattgta gcgaatctac cgtaaaagtc aactaaaatc
     3721 aataggatta ctaatatagt cacaataaag atcatcaaaa agcagcaaac ttcatcaata
     3781 gattgattga tgcgtgtttt tagcccataa ttgagagagc taagaacacg cgggtgatat
     3841 catatttttg ctgttgactt tttaaattta aaaacctaac cagccggtta actggtcagg
     3901 tttaaacaag ggagggcacc taagaagata ggtgcccagg agatttatga aaagggaaaa
     3961 caaggggaaa acctattctc ccaacggttt aggtaaaccg ctttttagga tgttttcatt
     4021 ctacgatagt aaccttaaag ttttcttaaa tgattcttta gaatgccttt atttttaatc
     4081 ttttttgaga gagctagctt cttgtcagcc ttgaaccttt ttcctaattt atggtatgat
     4141 aagaagataa tgaaaaaaga gattctcaaa gaactcttct agtctttacg aactatggat
     4201 gtagacttaa aacgtcaaaa tgatcaagct ttattagtgg atgttgttag caatcaagct
     4261 tatcaacggg ccaatacgat tgccaaatca aacgatttat gatattgctg tttaggaggt
     4321 ttatcttaga tgacacggtt attaaaacga tatcctatta ctattttcct tttagggtta
     4381 acaggcttaa tttttatagc aatgcaagtg gtatatggac atttagcaac tggtgttcaa
     4441 gctatttatc aagttggagg tatgtttgga ttactagtaa aagcaatgcc agatcagttg
     4501 tggcgattgg tgactccgat atttattcat atcggttttg gtcatttttt tgtcaatgga
     4561 ttaacactat attttgttgg tcaaatcgtc gaagacctct ggggctcaag ccgtttttta
     4621 ttactttatg ttttatcagg tgttatgggc aatgccttta ctttttggtt gactcctgaa
     4681 acagtagctg caggagcatc gacgtcgtta tttggacttt ttgctgctat tgtagtattg
     4741 agttttttgg gtaagaacca agcgttgaaa gatttgggca agtcatacca aacgttaatt
     4801 gtagtcaatt tactgatgaa tctttttatg ccaaacgtga gtatggcagg ccatattggc
     4861 ggtgtcgtgg gtggtgcgtt actgagtata gtatttccaa ctaagatgag agtgataaca
     4921 gtgaagaaaa ccaagcgaat gttggcttta gtgagctatg gtattatttt ggttggtgtg
     4981 ttagtgcttg ggtttttata aaaataagtg agtggttagc actgttactt ctcagatggc
     5041 tagtaggatg aaacgactca aaaaccataa aaataaggag ggattttccc tccttatttt
     5101 tatggttttg ttttctctag tgctttgccc agttggatga tgtaggcttt caagtcatct
     5161 ttgacttgag ggtgtttcag ggcataatcc aaagaagttt tcataaaccc aaatttatcg
     5221 ccaacatcat agcgtttccc tttaaactca cgagcaaaga cacgctgtgt cttattgagg
     5281 gtatcaatag catcagtcag ttgaacttca ttgcctgcac caggttcttg tttttctaaa
     5341 atactaaaaa tttctggtgt gagcaagtaa cgtccaataa ttgccaaatc actaggtgca
     5401 tcttgaggtt gtggtttttc aacaaaagta tccacactat agagtccttt tactgctttt
     5461 gcttgaggag caatgatacc gtagttagag acatcctcgt gaggaaccct catgacggca
     5521 atagttgcag catgagtttc ttcatagtca tcgatcaatt gtttggttaa tggttttaca
     5581 tttggatttg taatatccat cagatcatca cctagcatga caacaaaagg ttcattgcca
     5641 acaaaggtct ttgcttgaag aactgcgtct cctaatcctc ttggatgact ctgtcgaata
     5701 aagtgaagat taatagaagt ggtctcgtct actaacttta gaagttcgat ttttccttta
     5761 gcctgtaaat tatattctaa ttcaaaatta gaatcaaaat gatcttctat ggagcgtttg
     5821 gattttccag taacgataag aatttcttcg atacctgatt ttagagcttc ttcaacgata
     5881 aattgaatag ttggtttatc aacaataggt agcatctcct tggcaagggc cttggttgca
     5941 ggtaaaaaac gtgtgcctaa acctgctgcg ggaataatag cttttctgac tttggtcatc
     6001 agcacctcct tttattttag tgacgcatgg cttaatgcca ttcattttca gaacgaaatt
     6061 catttgacat catgccgagg atgctttctt tgatatcagc gccctcgtag atggacttat
     6121 aaatagctgt tgtaattggc atgtaaactc cgaggtcttg ggcaatttca taggctacct
     6181 tagttgttgc gatgccttcg ataaccatac ccatgttgcg ttcaatatct tctaattttt
     6241 caccacgtcc aagggcagct ccagcccgcc agttacgtga atggacagaa gtacctgtta
     6301 caattagatc accaacacca gataacccac tgtaggttaa cgggtcagca ccaagcttga
     6361 cgcctagacg ggtgatttca gctaacccac gagtaattac cgctgctttg gcattatctc
     6421 catacccaag tccatggaga gctcctgctc caacagcaat aatgtttttg agggcaccag
     6481 ctgtttcaac tccgatgacg tcggtattgg tataaagtcg gaagtagtgg ttactaaaga
     6541 gggactgaac atactttgca gcttcaatat cttttgaggc tgccgtgatg agagtaatat
     6601 cgcgaacgat agtttcttca gcatgactag gacctgatac aacgaccact tcactacgaa
     6661 aatgagctgg aatttcttct tctaggatag ttgataaacg ttcgtgggtc tctggttcta
     6721 gaccttttga ggcatgcatg accactactt tatgatctaa aatggcggca acttgtctag
     6781 caactaatcg tgtcacttta gttggcacaa caaaaagcac cgcatcaaca tctgataggg
     6841 cttgacctaa atcaagagtt gccgtaatgt ttttgtctaa cacaatgtct ttgaagtagt
     6901 gtctattggt atgcttggta tttatctctt caatttgatc aggaatattt ccccataaac
     6961 ggacgtcatg gccattgtca ttaagaacct gagaaagggc agttccccaa gaaccaggtc
     7021 ctaaaatagc tactttttgt tttgtcattc taaattctcc ttcaagagct agtcactcta
     7081 ttataacata acaccttgat tagctctatt agaaaaggta tatggtcttg gctaataagg
     7141 cgttcccaag agagctagct atattaataa tgttgtttaa taagcttcat caggtgagct
     7201 gttgtcatgg ctttgccctg ttctttttag ttatttaagt tcggaaactt atttttaaaa
     7261 ataaagatat tttaattgac aatagttatc tagagaacta aaatacttat catagaaact
     7321 attaaagaaa ataaggaggg aagagatgtc acaagtgata ggtgatttac gtgaattgat
     7381 acatcaaatc gaacaaatta gtgatgagat tgcaaaaaaa tatgatgtag agcatctagc
     7441 aggtcctcaa ggttatgttc ttgttttttt agctaaacac caaaatcaag aaatatttgt
     7501 caaagatatt gaaaaacaac ttcgtatctc aaagtcagtt gctagtcatt tagtgaaacg
     7561 tatggtcaaa aatgggttta tcaatgtgat gccttcccaa gtggataagc gttataagca
     7621 agtagtgtta gcgcaggttg gtagagataa attgcctttg ttgcgggagt gtcgtaagga
     7681 tatcgagcac tattttttaa aagaaattac aaaagaagag ttgctgacag cgaaaaaagt
     7741 aattgaacag ctcaagcaaa atatgctaac ttataaagga gacaacgatg cttaaagacg
     7801 caatcttacg ttataaatgg tatgctttag gctcattttt tatggttatt ggggttgttg
     7861 caagtagtct cttgcaacca cattatctta aatctgtgct aagcgctgtt attcaaaata
     7921 atcaagagaa aatttatgag gttgggaaac cattacttat tattgccctt ttagggttaa
     7981 tgtctggagc gattaatacg gttttagctg ctaaaatagc tcaaggtgta tctgctgata
     8041 tgagggagaa gacctttcga aaaatccaag acttttcata tgccaatatt gaagctttta
     8101 atgctggtaa tcttgtggtt aggctaacta atgatatcaa tcaaatccaa agtttagtca
     8161 tgatgatgtt tcaaattctc tttcgtttgc cgttcctttt tattggcgcg tttatcatgg
     8221 cagttcagac attccctcaa ttatggtggg tcattgttgt aatggttatc ttaattgccc
     8281 tcatcatggg tttggtgatg cgtcaaatgg gtcctcgttt tggtaaattt caacggttaa
     8341 tggataagat taatcgcatc gctaaggaaa atctccgtgg cgttcgcgtg gttaaatcat
     8401 ttgtacaaga acaacaacaa tacactaaat ttaaagaaac gtccaatgat cttttggcgt
     8461 taaatttatc tattggttat ggtttttcac taatgcagcc ggctttaatg ttggtgtctt
     8521 atttggcagt ttatgtttct ataaatgttg tttcgaccat ggtcgaaaca gatccaacag
     8581 tgattgggaa tattgcctct ttcatgactt atatgatgca aattatgttt tcaattattg
     8641 tggttggttc catggggatg caagttagtc gtgcttttgt ttctatggct agaattaggc
     8701 aaatattgtc tacagaacct gccatgacat ttgaaaatga aaaggaagaa accatctcag
     8761 gaagcattgt atttgacgat gttagtttta catatcctaa tgatgatgag ccaaccttga
     8821 agcacatttc ttttgctatt gaacctgggc aaatggtcgg catcgttggt gcaacaggat
     8881 ctgggaaatc aacgcttgta cagctcatcc ctcgcttatt tgatcctcaa gatggccaaa
     8941 ttttgttagg aggaaagccg attaaaaccc taagtcaaac aaccctgcgc caatcggttt
     9001 ccatcgtgtt gcaaaaggct attctctttt caggaacgat agctgataac cttcgccaag
     9061 gctcagcaaa agctgatatt gatgctatgc aaaaagccgc tcaaatcgcg caagcaaaag
     9121 aatttattga tcgcatggat agccgttatg agagccaagt tgaagagcga ggtagtaact
     9181 tgtctggcgg acaaaagcaa cgtctgtcta ttgcacgtgg tgtcattaat catcccaaaa
     9241 tccttatatt agacgattcc acttcagctc ttgatgcaaa atctgaaaaa cgcgttcaag
     9301 aagccttaag tcataaatta gaaggtacaa ctacagttat tgttgctcaa aaaatttcat
     9361 cggtagttaa ggcagataag atcttagtcc ttgaccaagg acagttaatt ggagaaggaa
     9421 cacacgcaga gttggtggct aataatgcga tttatcgtga aatttatgag acacaaaaag
     9481 gaagggagga gtagtgatga aaacagcacg ttttttctgg ttttatttta aacgctatcg
     9541 tttctcgttt actgtcattg ctgttgccgt tatcttagca acttatttac aagtaaaagc
     9601 tcctgtcttc ttaggggagt ccttgactga gttgggaaaa atcggtcagg cttattacgt
     9661 tgctaagatg agtggtcaga cacattttag ccctgattta tcagctttta atgccgtgat
     9721 gtttaagctt ttgatgactt atttctttac tgttttagct aatctaatat atagtttctt
     9781 actcacacgt gttgtctcac attcgaccaa ccgcatgcgc aagggcttgt ttggtaaatt
     9841 agaacgttta accgtcgcct tttttgaccg ccataaagat ggggagattc tttctcgttt
     9901 cacgagtgat ttggataata ttcaaaactc gctgaaccaa tccttggttc aagtggtgac
     9961 taatattgcc ctttacatcg gcctggtctg gatgatgttt aggcaagata gccgtttagc
    10021 tttgttaacc atcgcatcaa ccccagttgc tctcattttt ttagtgatta acatccgttt
    10081 ggcaagaaaa tacaccaata tccaacagca agaagtcagt gctttaaatg cttttatgga
    10141 tgaaaccatt tcaggacaaa aggctattat tgtacaaggt gtccaagaag atacgatgac
    10201 agccttttta aagcataatg aaagggttcg acaagccacc ttcaaacgcc gtctgttctc
    10261 aggacaatta tttccagtca tgaatggaat gagccttatt aacacggctg tcgtgatttt
    10321 tgtcggttca acaattgtcc tcagtgacaa atctatgcca gcagcggcag cgcttggttt
    10381 agtggttact tttgtacaat attcccagca atattaccaa cccatgatgc aaatcgcgtc
    10441 tagttgggga gaattgcagc tggcctttac cggtgctcac cgtattcaag aaatgtttga
    10501 tgaaaccgaa gaagttcgtc cacaaaatgc accagcgttc accagcttaa aagaagcagt
    10561 ggcgattaac cacgtcgatt ttgggtatct tcctgggcaa aaagttttat cagatgtgtc
    10621 aatcgttgca cccaagggca aaatgattgc cgtggttgga ccgacaggtt ctggaaagac
    10681 cactattatg aacttgatta accgtttcta cgatgtggat gcaggttcga ttacctttga
    10741 tggccgtgat attcgtgact acgatttgga tagtcttcgt caaaaggtag ggattgtgtt
    10801 gcaagagtca gttctttttt caggaaccat tacggataat attcgttttg gtgatcagac
    10861 cattagtcaa gacatggttg aaactgctgc gcgtgcgacc catattcatg actttatcat
    10921 gtccttacca aaagggtaca atacctatgt ctcagatgat gacaatgtct tttcaacagg
    10981 tcaaaagcag ttgatttcta ttgctaggac gctactgacg gaccctgaag tgttgatttt
    11041 ggatgaggcc acttcaaatg ttgatacggt taccgaaagt aaaattcaac gggccatgga
    11101 agctatcgtg gcaggtcgaa ccagctttgt cattgctcac cgcctcaaaa ccattttaaa
    11161 tgccgatcac attattgtgt tgaaagatgg caaggtcatt gagcaaggaa atcatcatga
    11221 gctattgcat caaaaaggct tttatgccga attgtatcac aatcaatttg tctttgaata
    11281 gatggcatca gctccgcttc ttagcggagt tttttggtga acaataaagc agtcaagctt
    11341 tctttcctga tatggtataa tagcaagcaa gagaaaaaac aattagaaag tagccacaat
    11401 gattgattta caagagattc tcgctaacat gaatcccaat caaaagataa attatgacag
    11461 ggtcatgcaa cagatggcca aggtttgggc taaggaatcc gtgcgtccca gtattttaat
    11521 gcatgtctgc tgcgccccat gctccactta caccctagaa tacctgacgc agtttgctga
    11581 cattacggtt tactttgcca attctaatat tcaccctaag gacgaatacc atcgcagagc
    11641 ctatgtgaca caacagtttg tctccgagtt caacgctaaa acaggcaaca ccgtgcagtt
    11701 tctcgaagcg gactatgtcc ccaatgagta cgttcgacaa gtgcgaggtt tagaagaaga
    11761 accagaaggt ggggaccgtt gccgtgtctg ttttgattac cgactagaca agaccgctca
    11821 aaaagcagtg gaactaggtt ttgactactt tgccagtgcc ctaaccatca gtcctcataa
    11881 aaattcgcaa accattaatg atgtgggcat cgatgtgcaa aaggtttaca ccaccaaata
    11941 cctgccaagt gatttcaaga aaaacaacgg ttatcgcaga tccgttgaaa tgtgtgagga
    12001 gtatgatatt taccgtcaat gctattgtgg ctgtgtctat gctgccaaaa tgcaaggtat
    12061 tgacttggtt caagtgaaaa aagatgccaa agcttttatg gcagacaagg atttggataa
    12121 tgatttcacc cacatccgct ttagttatcg tggggatgag atgtgatggc atgattaaaa
    12181 gtggtccgtt taaagttgac cgcttttttg ctattctttt acgaatagtc tataataaga
    12241 ggacatgatt gacttgaaag gatataccta tcataatgac aaaaattcgt ggtttcgagc
    12301 ttgtttcaag ctttactaat cctgatttac taccaaaacg tgagacgact catgcagctg
    12361 gttatgacct gtctgttgct gaggctgtga ccattgcccc aggggagatt aagttggtac
    12421 caacaggggt taaagcctac atgcaagatg gcgaggtgct ttacctctat gatcgttcgt
    12481 caaatccacg aaaaaaaggg attatcctga ttaattcagt aggtgtgatt gatgcagatt
    12541 attatggcaa tgaggctaat gaaggacata tttttgcaca aatgcaaaac attacagatc
    12601 accctgtgac attagctgtg ggtgagagga tcgttcaggg ggtttttatg ccttttttaa
    12661 ttgctgatgg tgaccaagcc agaggtgaac gaacaggtgg ttttggttcg acagggcagt
    12721 aaagcgattt aacaaaaatg cttatgacag cagacctatt ctttaagagt gggtttgtct
    12781 tgtctagggg agggagatta ctatggcaaa gaaaaaagca acctttatct gtcaggagtg
    12841 tggctaccag tcgccaaaat atttagggcg ctgtccaaat tgctcggcct ggtcttcttt
    12901 tgtggaagaa gtagaggtca aagaagtcaa aaatgcccgt gttagtctag ctggagaaaa
    12961 gtccagaccg gttaaactaa aagatgtgga taatattagt tatcacagaa cacagactga
    13021 catgtctgaa tttaatagag ttcttggagg gggtgtggtt cctggaagct tgattttaat
    13081 tggtggtgat ccgggtattg gaaaatccac cttactcttg caggtatcca cgcagctcgc
    13141 taataaggga acggttctct atgtgtctgg ggaagaatca gcagagcaga ttaaactgcg
    13201 gagtgagcgt ctgggtgata ttgacaatga attttacctc tatgccgaaa ccaatatgca
    13261 agctattcgc acagaaattg aaaatattaa accagatttt ttaattattg actccattca
    13321 gaccatcatg agccctgata ttacaggtgt tcaaggatca gtcagtcagg tgagagaagt
    13381 cactgcagaa ttgatgcagc ttgcaaagac gaacaatatt gcaaccttta ttgtgggtca
    13441 tgtcaccaag gaaggaacct tggctgggcc tcgtatgtta gaacacatgg tggataccgt
    13501 tctctacttt gaaggggaaa ggcatcacac ctttcgcatt cttcgggctg tgaaaaaccg
    13561 ctttggctcc actaacgaga tcggcatctt tgaaatgcag tctggcggct tggtagaggt
    13621 cttaaatcct agtcaagtct ttttagagga acgcttagat ggggcaacag gatcggctgt
    13681 cgttgtaacg atggaaggaa gtcgtcctat cttagcagag gtgcaatctt tagtgacacc
    13741 aaccgttttt ggcaatgcca gacgaaccac tacgggactt gatttcaatc gcgtaagcct
    13801 aataatggca gttttagaaa agcgctgtgg tttactctta caaaatcaag atgcctacct
    13861 caagtcggct ggtggtgtca aactagacga acctgccatt gatttggcag tcgctgtggc
    13921 tatcgcatcc agttataagg aaaaaccaac cagccctcaa gaagctttct taggcgagat
    13981 cgggttaaca ggtgagattc ggcgagtgac ccgtattgag caacggatta atgaagcagc
    14041 aaagcttgga tttaccaagg tctatgctcc taaaaatgcc ttgcaaggca ttgatatccc
    14101 tcaaggtatt gaggttgtcg gagtgactac ggttggtcag gttttaaagg cagtttttag
    14161 ctagaggatc agaaactaag agcaaatgag gaggaaattt gtcaagaaag tatcccaatc
    14221 atttgttttt taaacttttc gaacgatacc gaccgtctaa gaggttaatc cagctatttt
    14281 ccataaaaga tgttaagata agacaaacta acagcaaaaa tagctctgct gtttctcaaa
    14341 aggagtttat tgatgtctta ttttgaacat tttatggctg ccaatcaggt ttatgtggcc
    14401 ttgcatggga cagctcattt gcccttaaaa cccaagacta aagtagcgat tgtgacttgt
    14461 atggattcac ggctacatgt ggcccaagct ctggggctgg ctctaggaga tgctcatatt
    14521 ttaaggaatg ctggcggacg tgtcaccgaa gacatgattc ggtcgctggt tatttctcag
    14581 cagcaaatgg gaacgcgaga gattgtggtt ctccaccata cagattgtgg cgcgcagact
    14641 tttactaatg aaggctttgc caagcatatt catgagcact taggtgtgga tgtttcaggg
    14701 caagatttcc ttcccttcca agatgtggaa gacagtgtcc gcaaggatat ggctaagcta
    14761 agagcctcct ctcttatccc tgatgatgtc gttattaacg gtgccgtcta cgatgtggat
    14821 actggaaaga tgacacaagt aatggaatag ttagtagcca agttgtagag ctgaattcag
    14881 ctctttttta tttttaaaaa ttattgaaaa tattagacaa agaaataggg aagcgttata
    14941 ataattgtat caagttattc atacaaacaa ggagagtaag atggtcgaca atagaatgag
    15001 atttacgatt gatcaaaaca tgcaatttcc actagttgag attgatctgg aacatggggg
    15061 atcagtttac ttacaacaag gaagtatggt ttatcatact gagaatgtaa ccttgaatac
    15121 caaattgaat ggtaagggat ctggtttagg aaaattagtt ggagccatcg gccgttccat
    15181 ggtttctggt gaatcaatgt tcattacaca agctatgtca aatggagatg gtaaattagc
    15241 attagcaccc aatacaccag gacagattgt tgctttagaa ctaggtgaaa aacaatatcg
    15301 tctaaatgat ggtgcctttc tagccctaga tggttcagca cagtacaaaa tggaacgtca
    15361 aaatattgga aaagcacttt ttggtggtca aggcggactt tttgtcatga caactgaagg
    15421 ccttggtacc ttacttgcta actcgtttgg atctatcaag aaaatcacac ttgatggtgg
    15481 cacaatgact attgataatg cccatgttgt agcatggagc agagaacttg attatgatat
    15541 tcatttagaa aatggcttta tgcagtcaat tggtacaggt gaaggagttg ttaatacctt
    15601 tagaggtcat ggtgagattt atatccaaag tcttaacctt gagcaatttg ctgggactct
    15661 taaacgttat ctaccaacta gttcaaatta gtcaaaataa cataaaatca gctttttggg
    15721 gctgattttt ttcgcctaag caagtaaaat atgctataat aagaaagatt ttagaagaag
    15781 tctgaaaagg tcttggcaag ctcaaatcat catttgttgc ttgtttttcg tgccacagat
    15841 ttctgagtga tatatgtaac aggagaagaa gcatgtctaa accaattcgt gtgcgttatg
    15901 caccaagtcc aacagggctt ttacatatcg ggaatgcccg cacagcccta tttaattacc
    15961 tttatgcccg tcgtcacgga ggtactttta tcattcgtat tgaagatact gaccgtaaac
    16021 gtcatgttga agatggtgaa cgttctcaac ttgaaaacct aaaatggtta ggaatggatt
    16081 gggatgaaag cccagaaacg catgaaaatt accgccagtc tgaacgtcta gcactttatc
    16141 aacaatatat tgatcaattg ttagctgaag gaaaagccta taagtcttac gtgactgagg
    16201 aagaattggc agcagagcgt gaacgccaag aagcagctgg cgaaacgcct cgctatatca
    16261 atgaatttat tggtatgtca gctgacgaaa aagctaagta cattgctgag cgtgaagcag
    16321 caggtatcgt tccaacagtc cgtcttgctg ttaatgaagc tggtatttac aaatggacgg
    16381 acatggttaa aggcgatatt gagtttgaag gtggcaatat cggtggtgac tgggttattc
    16441 agaaaaaaga tggttaccca acttacaact ttgcggttgt tgtagatgac catgacatgc
    16501 aaatttcaca tgttatccgt ggggatgacc atatagccaa tacgccaaaa caattgatgg
    16561 tctatgaagc tcttggctgg gaagcgccag agtttggtca tatgactctt atcatcaatt
    16621 ctgaaacggg taaaaaatta tcaaaacgcg acaccaatac cctccaattc attgaagatt
    16681 accgcaaaaa aggttatatg cctgaagctg tctttaactt catcgccctt cttggctgga
    16741 atcctggcgg agaagaggaa atcttctctc gtgagcaact gattgccctt tttgatgaaa
    16801 atcgccttag caagtcgcca gctgcctttg accagaagaa aatggattgg atgagcaatg
    16861 agtatcttaa acatgcggat tttgaaacag tttatgccct ctgcaaacct ttccttgaag
    16921 aggcaggccg tttgacagaa aaagctgaga agctagtaga attatacaaa cctcaattaa
    16981 aatctgcaga tgagattatt ccattgactg accttttctt ttctgatttc cctgaattaa
    17041 cagaagccga aaaagaagtg atggcaggtg aaacagtttc gacagtcttg caagctttca
    17101 aggctaagtt ggaagctatg tcagatgaag acttcaaacc agaaaatatt ttcccacaaa
    17161 ttaaggctgt tcaaaaagaa acaggaatca aagggaaaaa cctctttatg cctattcgta
    17221 ttgcagtatc tggtgaaatg catggcccag aattaccaaa tacgatttat ttattgggac
    17281 gtgataaatc cattgagcac atcaaaaaca tgctataagg ctgtatcgtt cacctttatt
    17341 tttaaacgct ttaaggagtt aaaaagtcac gacaacgaaa gattgaagat taagttcttc
    17401 agtctttttt atcgttatct gagcttgcta ccttggtaaa gtaaaaaata gtatagttta
    17461 tttctatttt ggagattgga tttgaaagag aggtggtgag atgcttacgt gataatgaca
    17521 gcaaacggtg aggttttgat tagtgtgatt taaagaaatg gtttttatga atcaattagt
    17581 gtacaatatt tttt
//
LOCUS       DATURV010000030        17401 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DATURV010000030 DATURV010000000
VERSION     DATURV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17401
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.030"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            77..1486
                     /locus_tag="VCF75_001011"
     CDS             77..1486
                     /locus_tag="VCF75_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEP2546583.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQNHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            1664..3358
                     /locus_tag="VCF75_001012"
     CDS             1664..3358
                     /locus_tag="VCF75_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP2546584.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            3565..4419
                     /locus_tag="VCF75_001013"
     CDS             3565..4419
                     /locus_tag="VCF75_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEP2546585.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            4572..5912
                     /locus_tag="VCF75_001014"
     CDS             4572..5912
                     /locus_tag="VCF75_001014"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEP2546586.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQLLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            5890..6105
                     /locus_tag="VCF75_001015"
     CDS             5890..6105
                     /locus_tag="VCF75_001015"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEP2546587.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            6105..6977
                     /locus_tag="VCF75_001016"
     CDS             6105..6977
                     /locus_tag="VCF75_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP2546588.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            6970..7797
                     /locus_tag="VCF75_001017"
     CDS             6970..7797
                     /locus_tag="VCF75_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEP2546589.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKFKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            7784..8254
                     /locus_tag="VCF75_001018"
     CDS             7784..8254
                     /locus_tag="VCF75_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP2546590.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            8276..9937
                     /gene="recN"
                     /locus_tag="VCF75_001019"
     CDS             8276..9937
                     /gene="recN"
                     /locus_tag="VCF75_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEP2546591.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIVKEY
                     QLLTGDELSSGDLEAELKALEKQLVAAASELSVSRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISKKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTSEERVEEIARMLAGADMTEAALTQARELLAKH"
     gene            10145..11092
                     /locus_tag="VCF75_001020"
     CDS             10145..11092
                     /locus_tag="VCF75_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP2546592.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDR
                     LLNELDELDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPH
                     LRSLSDTALRSGEIRGHLDNKLDTLSVTATKLALTMAQKFDLTTHVYSIGESFSEVLA
                     AHYEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAV
                     AKRKIALPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            11308..12159
                     /locus_tag="VCF75_001021"
     CDS             11308..12159
                     /locus_tag="VCF75_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP2546593.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            12152..12994
                     /locus_tag="VCF75_001022"
     CDS             12152..12994
                     /locus_tag="VCF75_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEP2546594.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            12972..13559
                     /locus_tag="VCF75_001023"
     CDS             12972..13559
                     /locus_tag="VCF75_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEP2546595.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            13658..13933
                     /locus_tag="VCF75_001024"
     CDS             13658..13933
                     /locus_tag="VCF75_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEP2546596.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            14267..16129
                     /gene="cadA"
                     /locus_tag="VCF75_001025"
     CDS             14267..16129
                     /gene="cadA"
                     /locus_tag="VCF75_001025"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEP2546597.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYTSAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAEAASTHPISKAFLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYTLLDDIKI
                     ESKRAIKSLHDMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(16429..17364)
                     /locus_tag="VCF75_001026"
     CDS             complement(16429..17364)
                     /locus_tag="VCF75_001026"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEP2546598.1"
                     /translation="MVSTATQISHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLFFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
BASE COUNT         5561 a         2923 c         3693 g         5224 t
ORIGIN      
        1 tataggagat tacttttctt tttgttaaaa taaaaagaaa tagttgcatt atttgcatag
       61 aagaaaggga tctgttatgg tttcagtgat gtggtttcgt agagatttaa ggattgatga
      121 tcaaaaagct ttagctcatg ctattgctag tcaatcacca accttatgtg tgtttcaatt
      181 taacaaggag caattaaatc tgctacatag tcggaatcaa tctagtttta ttactagcgt
      241 tttggctttt agaaagttat taaaatgtga aggtattgac ttatatctaa tgtatggaga
      301 cttaatgacc tgttttgagc aattattgac acaattaaaa gactggacag atgtgtattt
      361 taactatgat gaatctggat atggtcgttt acgtgatcaa aaagcagcac aatttttcaa
      421 aaaaaatggg attgctgttc atacctacca aaatcactat ttacatggta gtcaagagat
      481 cattaaccaa tcaggtcagc cttataaagt ttttacgccc tactatagga tttggcaaaa
      541 ttatccgaaa gagactccta ttaaagtaga actatcgcaa ggcaggtggt taaatcttga
      601 gacaccagat gatgttttaa ggactgttga gagttttaaa gatgaaaaat atcaggatgt
      661 tgcaactttt gatgaagcgt caaaacaact aaatcgattt attcaagacc aattagcagc
      721 ttatcacgct aatcgagatt ttccagcgca gctagggacg agtcggttgt ctcctttttt
      781 aagaatagga gctattggca ttcgcacggt atatcacgct gtgcgtcaag caccaaatag
      841 tctagggcaa gctacttttt taaaagaatt agcttggaga gacttttaca atatggtcta
      901 tgtggcttat cctgatcaga aaactcagcc tatccaaaaa gccttttctc aaatcgaatg
      961 ggttaataat cctgactggt ttcagttgtg gaaagaaggt aaaacgggtt accctattgt
     1021 tgatgctgcc atgcttcagt tacaaaagac aggatggatg cataatcgtt tacgaatgat
     1081 agtagcttct tttttaacta aagatctttt atgtgattgg cgtttaggag agcagtattt
     1141 tcaacaacaa ctaattgatt atgatgctgc cagtaatatc ggaggatggc agtgggctgc
     1201 ttccactgga acagatgctg ttccttattt tagaatattt aatcctgtta ctcaaggtaa
     1261 gcgttttgat cccaaaggcg agtttattaa agcgtatttg cctcaactag aacacgttcc
     1321 agagaagtat ctccacgaac cgtggaagat gccaaaatac cttcaagaga gtgtatcctg
     1381 tattattggc acagactatc ctcaaccaat tgtcgatcat gctaaacaac gtgaacaagc
     1441 tattgcaaag tatgagtggg caaaagaaaa agcaaaaata gaatgactgt tctcattata
     1501 agagtttaaa caaaaagtta tcatgaatta agattattta atttaaaaaa taagttgact
     1561 ttatttagat tatttgatac tattcagttg tctcccttag agaaagcgat ttcttgtttt
     1621 ggagctgatt tttaccattt ggtcttagaa taggatgagt aatatgactt acaacgaggt
     1681 atatcaagaa tggttgcaca ataatgatct tagtgatgat attaaagcag atttagcagc
     1741 cataaaagac aatgaggctg agattcaaga tcgtttttac aaaacacttg aatttggaac
     1801 agcagggcta agaggaaaac ttggagcagg caccaatcgc atgaatacct atatggttgg
     1861 taaggcagca caagctttag ctaatactat tattgatcat ggacctgaag cggttaaaaa
     1921 gggcattgcg gttagttatg atgttcgcta tcaatctaga acatttgcag aattaacatg
     1981 ctctattatg gcagctaacg gtattaaggc gtacctttat aaagggattc ggccaacacc
     2041 aatgtgttcg tacgctattc gtgctttggg atgtatctct ggtgttatga ttacagctag
     2101 tcacaatccc caagcttaca atggttataa agcttattgg caagaaggtt ctcaaatttt
     2161 ggatgacatt gcggatcaaa ttgcacaaca catggctgca ctaactcagt atgaggaaat
     2221 caaacaaatg ccttttgaaa aggctctgga cagtggactt gttacttata ttgatgagag
     2281 tattgaagaa gcatataaaa aagaagtgct tggtttaacg attaatgata ctgacattga
     2341 taagtcagtc cgagtagtct ataccccttt aaatggtgtt gggaatctac cagtgcgtga
     2401 ggttttaaga cgccgcggtt ttgaaaatgt ttatgtggta cctgagcagg aaatgcccga
     2461 tcctgatttt acaacagttg gctatcctaa tcccgaagtc cctaaaactt ttgcatattc
     2521 agaaaaatta ggtaaggcag ttgatgctga tattttgatt gcaactgatc cagattgtga
     2581 ccgtgtggct ttagaagtta aaaatgcagt cggtgactat gtttttctca atggcaataa
     2641 aatcggagcg cttttatctt actatatctt ttcgcaacga tttgacttag gcaatttacc
     2701 ggctaatcct gttttagtga aatccattgt aacaggagac ttgtcacggg ctattgctag
     2761 ccattatggt attgaaaccg ttgaaacatt gactggtttt aagaatattt gtggcaaggc
     2821 aaacgaatat gatgtgacga aacagaaaaa ctacctgttt ggttatgaag aaagtattgg
     2881 tttttgctat ggcacttttg ttcgtgacaa agacgctgtt agcgcctcta tgatgattgt
     2941 agaaatggca gcttactaca agaaaaaggg gcagaattta ctagatgttt tgcagactat
     3001 ttatgcgaca tttgggtatt ataatgaacg tcaaattgcc ttggagcttg aggggataga
     3061 aggacaaaaa cgcattgcga ggataatgga agatttcaga cagacaccaa tagctagtgt
     3121 tgcagagatg gcgttagaca agacaattga ttttattgat ggctatcaag attttccaaa
     3181 gcaaaactgt ctcaaatttt atttggatga tggttcttgg tatgcactta gaccatcagg
     3241 aacagagcct aaaataaaat tttatttata tactattggc caaacacaag aaaatagtgc
     3301 aacaaaatta gatgctattg aagcagcgtg tcgcacaaaa ataaatcagg ttaattaaac
     3361 atcaaaaatg gataacactt ttacactatt ttccatataa acaactacaa aaggaggtga
     3421 gacttttagg caccttcttt ttcttgtagg tgccactaat caatttaatc gactcaaatc
     3481 aatactaaaa aataagtaag agtataaaga acaggcatct atttttttgt tataatagaa
     3541 aaatgaaatt aaagggagat gtcaatgaca gaactaattg atggtaaagc cttagctcaa
     3601 aagatgcaac aagagttagc agctaaagtc aacaatctaa aacaaaaaaa aggaattgta
     3661 ccaggcttag ccgttattct tgtaggtgat gatcctgcta gtcaggtgta tgtccgtaat
     3721 aaagagcgtg cagctcttac tgtaggtttt aaaagtgaga ccgttagatt atcagaattc
     3781 atttgtcaag aagagcttat tgcagtaatc gaacgttaca atgcagataa cactattcat
     3841 ggtattttag tgcagctacc cctgccaaat catattaatg ataaaaaaat tattctcgcc
     3901 attgatccca aaaaagatgt ggatggtttt cacccgatga atacaggtca cctttggtca
     3961 ggacgtccct taatggttcc ttgtactcca tcagggatta tggaattgct tcgagaatat
     4021 aatgttaacc ttgaaggtaa acatgccgtc attattggca gatcgaatat cgttggaaaa
     4081 ccaatggcac agctcttact agacaaaaat gcaacagtca cgttgacaca ttcaagaaca
     4141 cgtcaattag aagaagtatg tcgctgtgca gatgtgttga ttgtggcaat cggacaaggt
     4201 catttcataa caaaacaata tataaaagat ggtgcgatag tgattgatgt gggaatgaac
     4261 cgtgatgata atggcaagct aattggagat gtggcctttg atgaggtggc agaagttgca
     4321 gcgaaaatca cccctgtacc aggaggtgtc ggtcctatga cgattgctat gttgctagag
     4381 caaacttatc aatctgctct ccgtagtact cataaatagt tatcattaga caattcaaca
     4441 taagagaata taagaagtca gcaatggctt ccttttttgt ttttctgctc actatttatc
     4501 cagaaaaatg gtataattgc attagtatgc aaagggacta tgtcaggtcc aactaagtga
     4561 aaggtggctt gatggcagat tatttaaccg tcactcattt gacgaaatat ttaaaattaa
     4621 aatttgaccg tgacccttat ttggaacggg tttatctgac tggtcaagtg tccaattttc
     4681 gaaaacgacc aactcatcaa tatttttcct taaaagatga aagtgctgtg attcaagcca
     4741 ccatgtgggc aggagtctat aaaaaactag gatttgacct agaagaaggc atgaaaatta
     4801 atgtgattgg gcgagtccaa ctttatgaac cgagtggttc ctattctatc gtgattgaaa
     4861 aggcagagcc agacggcata ggtgccttgg ctttgcagtt tgaacaatta aagaaaaaat
     4921 taactgcaga aggttatttt gagcaaaaac acaagcagct cttaccacag tttgtgtcaa
     4981 aaattggggt cattacaagt ccaagtggcg ctgtgattcg agatattatc acaactgttt
     5041 cacggcgttt tccaggggtt gagattttat tattcccaac taaagtacaa ggtgatggag
     5101 ccgcccaaga agtagtggcg aatattcgaa gagccaacca aagagaggat ttggacttgc
     5161 tcattgttgg ccgtggaggt ggctcgatag aagacctttg ggctttcaac gaggaaatag
     5221 tagttcaggc tatctttgaa tcgcaacttc cagtgatttc gagtgtgggt catgaaactg
     5281 atacaaccct ggcagatttt gtggcagatc gcagagcagc cacaccaaca gcagcagcag
     5341 agttagcaac gcctattaca aaaacagatc ttatgtcttg gatagtagag cggcaaaatc
     5401 gttcttatca ggcctgcttg aggcggatta agcaacggca agagtgggtg gataaactat
     5461 cacaatctgt tatttttagg caaccagaac gattatatga tgcctacttg caaaaaattg
     5521 atcgccttag catgaccctg atgaatacga tgaaagatcg tctgagctcc gccaaagaaa
     5581 acaaggttca gttggaccat gccttggcca atagtcaatt acagacaaaa atcgagcgat
     5641 accaagaccg tgttgctacc gctaagcgtt tactgatggc taatatggct agtcaatatg
     5701 atagtcagct ggctcgcttt gaaaaagcac aggatgcttt gctatcactg gatgccagcc
     5761 gaattatcgc ccgtggttat gcaatgattg aaaaaaatca agcactggta gcctctgtaa
     5821 gtcagataac aaagggtgat cagttaacca ttaagatgcg tgatggacaa ttagatgtag
     5881 aggtaaaaga tgtcaaaaac gaaaacattt gaagaaaatt tacaagattt agagactatt
     5941 gtgaacaaac ttgaaaatgg ggatgtccct ttggaagaag ctatttcaga atttcaaaaa
     6001 ggaatgcttc tctcaaaaga acttcaaaag accttacaag cggcagaaaa aacactcgtc
     6061 aaagtgatgc aagctgatgg cacagaagta gatatggatg attaatggac aaattagcta
     6121 gaattgacga agccattcgt cgctactata agacgactag taacggtgta tctgaagagt
     6181 tgattgatgc tatcttgtat tctgttgaca gtggtggcaa gcgcattcga ccacttatct
     6241 tattagagat gattgaggga tttggggtat cgttacagaa tgctcatttt gatttggctg
     6301 cagcccttga aatgattcat acaggaagtc tgattcacga tgatttgcca gccatggata
     6361 atgatgatta ccgacgtggt agactgacca atcacaaaca atttggagaa gctacggcca
     6421 ttttggcggg agatagctta tttttagacc cctttgggtt aatagctcaa gctgaattaa
     6481 atagcgaggt taaagtagcc ttaatccaag aactttccct agcttcagga acttttggca
     6541 tggttggtgg tcagatgcta gatatgaaag gggaaaatca agctttgagt ctccctcagt
     6601 tgtcactgat tcacctcaat aaaaccggaa aattattaac ttttcctttt aaagcagcag
     6661 ctcttgtaac agaacaggct attactgttc gtcaacaact agagcaggcg ggaatgctca
     6721 tcgggcacgc ctttcagatt agggatgata ttttagacgt gacagctagc tttgaagatc
     6781 ttggaaaaac gcctaaaaag gatttatttg cagaaaaagc tacttaccca agtttactgg
     6841 ggttagaagc ctcctaccaa ctgttgacag agagtttaga tcaggctttg acgatttttc
     6901 agacactcga aagcgatgta ggctttaagc ctcaaataat tacaaaactg atagaagggt
     6961 tacgacttaa tgcctaaaga aagagtagat gtgttagcct ataagcaagg attatttgag
     7021 accagagagc aagctaagcg tggtgttatg gcaggcttag tggtctctgt gattaatggc
     7081 caacgctatg acaagccagg tgacaaaatt gacgatggga ctgagttaaa acttaaaggt
     7141 gaaaaattca aatacgtcag tcgtggtgga ttaaaactgg aaaaggggct gcacgttttt
     7201 ggtgtatcgg ttgctaatca aattgggatt gatattggcg cttcaacagg tggttttacc
     7261 gatgttatgt tacaagatgg ggccaaacta gtctatgccg tcgacgttgg gactaaccag
     7321 ttggtatgga aactcagaca agatccacga gtaagaagta tggaacagta taactttcgt
     7381 tatgctcagc cagaggactt taatgaggga cagcctgtat ttgccagtat tgatgtgtcc
     7441 tttatttctc tcagcttgat tttgccagcc ctgcataacg ttttgtcaga tcagggacag
     7501 gttattgctc tcattaaacc gcaatttgaa gctgggcgcg agcagattgg taaaaaaggc
     7561 atcgtcaaag acaaacagat tcatgaaaaa gtgattcaaa aggtcatgga ttttgcctca
     7621 ggttacggat ttacagttaa gggacttgat ttttccccta ttcagggtgg tcatggcaat
     7681 atcgaatttt tagctcatct tgctaagtca cagacacctg aaacgttagc cccgcatttg
     7741 attcagaaag ttgttgccaa ggcacataag gagtttgaga aacatgaaaa agagtgaacg
     7801 acttgaatta atcaaaaaaa tggttttgac gcatccaatt gagacgcaac atgatctctt
     7861 aagattattg gcagagcatg ggttagaatt aacccaggct actatttcaa gagacatgaa
     7921 tgaaattggt atagtaaaga ttccttctgg cagtggacgt tacatctatg gtctttctca
     7981 agatagtgga aaaaagatcg ttcaaggacc tagatcgata aagagcacta ttttagctgt
     8041 atctgacaaa acaaaaggtt tagaacaaca cctctattta aaagtcgtac ctggcaatag
     8101 taaattaatc aaacgttatt tattagcaga tttttcaaaa gctattttta gtcttattgc
     8161 tgatgatgat agtttactgt taattgcaaa gtctccttca gaagcagata tgatacgcca
     8221 agaaattttg ctttggatgc agggtataac ctagaaaaaa ataggggaga aagtgatgtt
     8281 attagaaatt tctattaaaa attttgctat tattgatgaa atttccctaa attttgaaaa
     8341 tggtatgacg gttttgacgg gtgaaactgg agcaggaaag tctattatca ttgatgctat
     8401 gaatatgatg cttggtgcgc gtgctagtac agaagtgatt cgtcgtggag ctaataaagc
     8461 agaaattgag gggttctttt cggtagatgc taccccagaa ctggttgcgt gtctggaatc
     8521 atcaggtatt gctatggaag aagaactgat tattcgccga gatatctttg ccaatggcag
     8581 aagcgtgagc cgcattaatg gtcagatggt taacctagcc accttaaaac aggtcggaca
     8641 gtttttggta gatattcatg ggcaacatga ccaagaagaa ttaatgcgac cacagctcca
     8701 ccagcaaatt ttggacgctt ttggtgataa ggcttttgag caattgaaag agaactatca
     8761 gcttattttt gatcgttata aaagcttgcg tcgccaggtt attgacaaac aaaaaaatga
     8821 aaaggaacac aaagatcgta ttgatatgtt ggcctttcag atagcagaaa ttgaagctgc
     8881 tgccttgagt cgaggtgagg acgaccggtt aaatcaagaa cgtgatcgct taatgaacca
     8941 caaacaaatt gctgataccc tgaccaatgc ctacgtcatg ctagataatg acgatttttc
     9001 aagtctatcc aatattcgct ctagcatgaa tgacttacta tcaattgagc agtttgattc
     9061 agaatacaaa gggatgtcga cttcgatttc tgaagcctat tatattcttg aagaagtgag
     9121 caagcaatta tcagatacca ttgaccaact ggattttgat ggtgggcgat tacaggaaat
     9181 tgaatttcgc ttggatacac tcaatagctt aacccgtaaa tacggtggta atgtgaatga
     9241 tgtgcttgac tactacgata atatcgtcaa agaataccag ctattaacag gcgatgagtt
     9301 gtcttcaggg gatctagagg cagaattgaa agccttagaa aaacaattgg ttgctgctgc
     9361 ttcggagtta agtgtctctc gccatcaatt ggcggagcaa ttagaagctg agattaaagc
     9421 tgaacttaag gaactttata tggaaaaagc agatttcaaa gttcacttta ccacatcaaa
     9481 attcaatcgt gatggtaatg aaagcttgga attttacatc tccaccaatc caggtgaagg
     9541 attcaagccc cttgtcaagg tggcttcggg cggcgaatta tcacgcttga tgttggccat
     9601 caaggcagct atctctaaaa aagaagataa aacgagtatt gtttttgatg aagtagatac
     9661 aggtgtttct ggtcgagttg ctcaagccat tgcccaaaag atttataaaa ttgggcgaca
     9721 tgggcaagtt ctcgctattt cacacttacc tcaagtcatt gccatcgctg attaccagta
     9781 ttttatttct aaagagagta aagaagagtc cactgtttcg aaggtaaggt tgttaacttc
     9841 tgaagaacgt gtggaagaaa ttgccagaat gcttgctggt gcagacatga cagaagctgc
     9901 tctcactcag gctcgtgaat tactggccaa acattaataa acagaaaaaa ctgtgactat
     9961 tcaatagaat agtcttttga taagaaagag aaaccgtcgg attagcctgt tgttagattc
    10021 tggagcctta gcgctccgat gccatctagc aggacgtttg gcggtttctt tttgctcaac
    10081 tgtcttgaca aacccctgat aaatagtaga atatataaga gaattttaag ctgaggggat
    10141 agaaatgact agtaaaaaag cgtgtttatc aagtatcatt gtgttagcaa gtttaacgtg
    10201 tggaaatgat actgttagtg ccaatcatct ctcagcaact ggagataagt ttgatgattg
    10261 ctcaacactt gttgaaaaag atgtggcccc taaagatgaa cttgagatgt tagcatggtc
    10321 ctcgtctcaa acaactgatg atgctgacag agactatgaa gattttctcg atgatgattc
    10381 ttttaatgat gattctttta tttctcaaaa tgaaactgat aagatgtttg agaatttaac
    10441 tgatgatagg ttattaaatg aattagatga attagatgaa ttagatgaag aagatacaat
    10501 tgagccagag caaaatgtaa taatgcctag tgacgatgag ctatttgatt taactgatgc
    10561 tgttgagaca cgccttactg tttctagtgc tccccattta gaggctgaat tgccgaaacc
    10621 acatttgagg agcctatcag atacagcact gcggtctggt gaaattagag gacatttaga
    10681 taacaaactg gacactttgt ctgtaacagc tacaaagtta gcattaacga tggctcaaaa
    10741 atttgatttg acaacgcatg tctattctat aggtgaaagc tttagtgaag tattagctgc
    10801 tcattatgaa gacagaaaag cagaatcagc tttttctaag aaaaagagat ttcaccttcc
    10861 tattgctact ccagatgttg ttatagagga gttaaggcgc ctagtctctt ctattggaag
    10921 ttcaaaagaa gatgtttcag ttccttataa tcggaagcta ggtatggcag ttgcaaaaag
    10981 aaaaatagcc ctgccacaaa cgggagagga gttctcttat tatccagctt tacttggttt
    11041 aatgatatta ggattaacgc cgattatgat accaaagaag ataaataatt aggtaataag
    11101 ttattgtagt tgtaaaagac ggggcaaatg ttcaagcgtg aaaaggttga cttttgcctc
    11161 gttttgtatc tcgttattgc taaggcattt tcttcgagca gctagtgctc gccataccta
    11221 tctaccgagt gattattcaa tagcactacc agttttgctt tatttagtaa ggagtgttag
    11281 aataaagagg tgagaaacta gtttagaatg gaaaaataca tgggaactat aaaaattgtt
    11341 acagattcat caataactat tgaaccagaa ttaattaaag ctttagatat tactgtagtg
    11401 cctttatctg tcatgattga tagcaaatta tattctgata atgatttaaa agaagaaggt
    11461 cattttttga gccttatgaa ggctagcaaa tcccttccca aaaccagtca gccaccggtt
    11521 ggtctctttg ctgaaactta cgaaaatttg gtcaaaaaag gagtaactga cattgttgct
    11581 attcacctct caccagcttt atcaggtact attgaagctt cccgtcaggg ggctgaaatc
    11641 gctgaggcac ctgttacagt tttagattca ggatttactg atcaagccat gaaatttcag
    11701 gtcgttgagg cagcaaagat ggcaaaagcg ggtgctagtt tgaatgagat tttggctgct
    11761 gttcaagcta tcaaatcaaa aacagagctc tacattggtg tatctacctt agaaaattta
    11821 gtaaaaggag gtcgtattgg tcgtgtgacg ggtgttttga gctcgctact taatgtcaaa
    11881 gttgttatgg cacttaaaaa tgatgagtta aaaacgcttg tcaaaggtcg aggtaataaa
    11941 acatttacga aatggttaga tagttacctc gctaaaaata gtcatcgccc gattgcagag
    12001 atagctattt cttatgctgg tgaagctagc ctggcattaa ccttaaaaga aagaatcgca
    12061 gcttattaca accactctat ttcggtttta gaaacaggct ctatcatcca aacacataca
    12121 ggagaaggag cttttgcggt tatggttcgt tatgaataat cgtcatttat ttagtgggat
    12181 atttttcttt gttattagtt tatgtctggc ctttttattg ctaaatatta ttatccctaa
    12241 gtcaaattca cgtttgaaaa agagtgattt tctgaaaaaa gaacaagtag ctatccaata
    12301 tgttgctata ggagattcat tgacagaagg agtaggtgat ctaactcatc aaggtggttt
    12361 tgttcctttg ttaacgaatg atctcagtga atattttaag gctaatgtta atcatcaaaa
    12421 ttacggcgta tctggtgata ccagtcaaca aattcttgat aggatgataa aacaaaagca
    12481 gatacagtta tctttaaaaa aagcagatat aatgacgtta accgttggtg gtaatgatgt
    12541 tatggcagtt attcggaaga atttagcgga tttgcaagtt tctagtttta gaaagccagc
    12601 tcgtcagtat caaaaacgat taagacagat tatcgagtta gccagaaaag ataataaaga
    12661 tcttcctatt tttattttag gcatctataa tccgttttat ttgaattttc cagaactaac
    12721 tgatatgcaa aaagtgattg atgactggaa taccaaaact aaggaggttg ttggagaata
    12781 cgatcgtgtg tactttgtgc caataaatga cctcttgtat aaagggataa atggacaaga
    12841 aggaattgtc cattcttcag gagatcaaac tacaattgtc aatgatgcct tgtttactgg
    12901 ggaccatttt cacccaaata atactggcta tcaaatcatg tcaaatgcag taatggagaa
    12961 aataaaaaag catgaaaaaa aaatcaaacc ttaattggtg gaagtggagc ttcttatgtc
    13021 tgcttgcttt taatactgcc tttttgatgg ttattgcaag tcgtctcatt caagtaagag
    13081 aacctgagtc agaactgatt gctaaaaaac cagttaaaaa tatcaaaatt ggtacatttg
    13141 tcacaaccag agaacagctt aacgaaactg tagcaagtta tcttaaagat tatcagacta
    13201 aaaaaatgtc ttataaattt tatgcgacat cttcttctat tttgtttgaa ggaacttatc
    13261 aattattagg gtatgaagtc cctttatata tctattttca acctcatcgt ctagaaaatg
    13321 gagctgtcca actacaggtg atctcttttt cagtgggaac tctgccactc ccagaaaaag
    13381 atgttttgca gtatttgaaa tcaagctaca aattgccaag ctttgttaaa gtgatgccaa
    13441 atcaatcagc tatcgttgtt aatttacaag atatccaaaa tgatgctaag gtttatttaa
    13501 aagccaagaa aattgattta tttaatgatg aaatcagttt taacatctat aagaaataag
    13561 ctcaaataac gttaaatact tgacctaatc gcttttttta gatatgataa tatctgttaa
    13621 agcattaagc ttactaatta tttggaggat ttgttaaatg gctaacaaac aagatttaat
    13681 cgcaaaggtt gcagaagcaa ctgaattgac taaaaaagat tcagcagcag cagttgatgc
    13741 cgtgttttct acaatcgaag ctttccttgc tgaaggtgaa aaagtacaat tgatcggttt
    13801 tggtaacttc gaagtacgcg aacgtgcagc tcgtaaaggt cgtaacccac aaactggtgc
    13861 agaaattgaa attgcagctt caaaagttcc agccttcaaa gctggtaaag ctcttaaaga
    13921 cgctgttaaa taattcgtct agaaaaatct tgttgctatc gatgtttatt gatagcgaca
    13981 aggttctttt tttatatttg gggcataaag ttagtagttt tctcttcagt taagccaagt
    14041 tctaaagcat gcactctata atggtcagtc atccttctat tagtatatca tttccgcagg
    14101 tagggggagt ttttttcata tttgcaaata gttgaagtta ggatttttga aagcaaaagg
    14161 taagtttatt tagaatgttt ctaaattact tgacttattt atttagaatt attataatta
    14221 atatatctta aagattgaat aacttttaat tgcgagggaa ctaaaaatga caatcagaca
    14281 atggatggca gaccatcttc atttgatgga gactttagcg tgtttggtat taataataat
    14341 tggcctagcc tttttacact catttccaca agtggcttcc gctattttca ttacagcctt
    14401 cttgattgga ggatatacgt ctgcaaaaac gggtatattg gatttggtga agaacaaaca
    14461 cttgtcagtg gatattttga tgattttggc agctatcggt gctggaatta tcggctattg
    14521 gctggagggt gctctgctta tttttatctt ttcgttgtcc aatacgcttg aagaaatggc
    14581 catggaaaaa agtaaggatg ctatttcagc cttgatgtcc ttgacgccag atacagctcg
    14641 tcaataccaa gaagatggcc atattttaga agttgagacc agatccttga gtgttggtga
    14701 ccgtttacaa gttcgtaaag gcgaagctgt tccaattgac gggcagttgc ttagtccttt
    14761 tggtcaattt gatgaatcta tggtcactgg tgagcctatc actgttgata aggcagaagg
    14821 ccaggatctg attgggggaa ccattaacca aggacaaacc atagatatgt tggttactat
    14881 tgaaaatgac gatactctct ttgccaagat tattaatctg gtggaatctg cccaagaaaa
    14941 gaaaagcaaa actgccacct ttatcgaaag cttagaagat ggttatgtca aatttgtgct
    15001 cgtccttatt cctgccttta tcctctttag ccactttgtg ctttcttgga cttggttggc
    15061 tgctttttac cgagggatga ttctcttaac agtagcttca ccatgtgccc taattgccag
    15121 ttccacacct gctagcttgg ctgctatttc tcgtgcagcc aggaagggat tgattatcaa
    15181 aggaggggat attgtcgata acatgggaga tattaaggct gttgtcatgg ataaaacggg
    15241 aacgctcacc caaggaaaac cttctgttgt gaatgctcat tatttggaag atgagttgct
    15301 cgtgaataga ctggtaaaag gagcagaggc tgctagtacc catcctattt ctaaagcctt
    15361 tcttgaatac actgaaaaat tggagccact gacctttgac cacttagaag aaatttctgg
    15421 gaaaggtttt cagggcttct atcaagggca agaatggcga attggcaaga aaacattcat
    15481 tttggaaaag gttcaagacc tatcagcttt tgaagaaact attcaagtgg aagaaaatca
    15541 agggaaaacc ctaatctttg tttcacgtga ccatcaattg atagcttact ataccctctt
    15601 ggatgatatc aaaatagaat caaaacgtgc tattaagtct cttcatgaca tgggaatcaa
    15661 aacagtcatg ttaacaggtg accaagaacg aaccgccaat tatgtggcac aaaaacttgg
    15721 tattgatgaa gtggtagcca actgtatgcc tcaagataag gtggctaagt tagcagaatt
    15781 aaagaccaaa tatggttttg tggccatggt aggagatggt attaatgatg ctcctgccct
    15841 tgctcaagca gatgtttctt atgctattgg atcaggaaca gatattgcaa tggaaagtgc
    15901 agacagtgtg attatggatg acttgactcg tattccattt tcgattcaac tttcccgcaa
    15961 aatgaagacc attatcaaac aaaatattgt ttttgcctta tctgtgatta ccttattgat
    16021 tttagccaat gtttttcagg tagttaactt gccgcttggt gttgttggac acgaaggctc
    16081 aacgatttta gtgattttaa atggcttgcg tttactttct tttaaataat ttagaaactt
    16141 gcttgaaaac ttgaaagtgt ttataataat agtaagactt atgaaagaga aaaccgccgt
    16201 atgtttacta ttatgatcat tggtttagct gtattttcaa taattaaaat cattctattt
    16261 ttctattttt atcagcaatt tgagcgcaag caaaaaagaa aagttgaatc aaacaaccag
    16321 caaagacagt cccaagaaag tgacgctgtc ccttcaaaaa aattcccatt ttaaaaagct
    16381 tggaaagtgt acaaagtgca catttccaag cttttttgac tattaaggtt aatctttgta
    16441 acgtaagttc ccacgaaaat cgtctaagct ttggtagcct ttttctacca taatcgtttt
    16501 tagttcctta gtgacccgtt caaagatagc tggcccttct tgatgaaggg ctgttccaat
    16561 ttggaccatg cttgcgccac aaagaatatg ttcaaaggca tcgcgtcctg ttttaactcc
    16621 ccctgtacca ataatgtgga tagaaggttt taagcgtttg taaaacgcat ggacattggc
    16681 tagagcagta ggtttgatgt aatctccacc tataccacca aagccatttt taggcttgat
    16741 gaggacttgc tcatcctcaa tgaccagccc atttccgata gaattgacac agttcacaaa
    16801 gaaaaggggg tatttattga aaatcgcagc cgcttgatca aaatgcacaa tgtcaaaata
    16861 aggaggtaat ttgataccaa gaggtttagt gtaataggta aagatgttct ctagaagttg
    16921 atcagtggtt tcaaaatcat aagcaatctg tggttttcct ggaacgttcg ggcaagaaag
    16981 gtttagttcc actagaccct cataatctga agccatgatg gcttttaaaa tggtttcggt
    17041 ttcagtagga gatagcccga caacggagag aaaatgaggt ttatgttgcc cggttttggc
    17101 aagatcactg acaaagtcta agtagtagcg aaaaccatta ttagggagtc ccattgaatt
    17161 gatagaacca agacgagtat ctgcgtagcg aggttctgga ttacctggac gaacttcaag
    17221 cgtccctgtt ttggtgacaa aggaagcggc ttgagacttt tcaacctcca taagctcttc
    17281 ttttgtcatg cagtaaacac cagcagcatt catcaaacag ttatcaaaag agaaatgact
    17341 aatttgtgta gcagtagaaa ccattatttc ctccatattt tagacaaatt ttttctattc
    17401 t
//
LOCUS       DATURV010000031        16467 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DATURV010000031 DATURV010000000
VERSION     DATURV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16467
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.031"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..560
                     /locus_tag="VCF75_001027"
     CDS             <1..560
                     /locus_tag="VCF75_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEP2546599.1"
                     /translation="SEKGIMVIRYSDRYIFTNGGTLRIDLKDFFSGAHSDPRNYLIQE
                     IFRFLNLCEKAGTGIPKIMEAVKESRLKYPKLRTQLDSVELTLWDTSLIDNLYIDNEY
                     EKKILELIIENRFITRETLEEKLKVHKNTILKYLKKLVEKQAIDKFKSGRQYVYFIAQ
                     NQDPEFQKYNHIDAMYSLLEEMKRK"
     gene            655..1551
                     /locus_tag="VCF75_001028"
                     /pseudo
     CDS             655..1551
                     /locus_tag="VCF75_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481078.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1646..1882
                     /locus_tag="VCF75_001029"
     CDS             1646..1882
                     /locus_tag="VCF75_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEP2546600.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            1875..2573
                     /locus_tag="VCF75_001030"
     CDS             1875..2573
                     /locus_tag="VCF75_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEP2546601.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLKQGYHVYGVDKSDKPDLNGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLAEWVARETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            2649..3608
                     /locus_tag="VCF75_001031"
     CDS             2649..3608
                     /locus_tag="VCF75_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP2546602.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLVTSKYEAILGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKTTIQGNKGSLAVLGATNTLPQVQLSLYGHEAQVVNHNKH
                     DHRMYEEFVAFKDMIDQKDFEKVNQALEHSRAVMAVLERAVHS"
     gene            3941..5278
                     /locus_tag="VCF75_001032"
     CDS             3941..5278
                     /locus_tag="VCF75_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2546603.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGILVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISILEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            5451..6833
                     /gene="glmU"
                     /locus_tag="VCF75_001033"
     CDS             5451..6833
                     /gene="glmU"
                     /locus_tag="VCF75_001033"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEP2546604.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGQGSIITNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDNALTAAGSTISKTVPADSIVIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            6864..7418
                     /locus_tag="VCF75_001034"
     CDS             6864..7418
                     /locus_tag="VCF75_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP2546605.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIESVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQIANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            7418..7669
                     /locus_tag="VCF75_001035"
     CDS             7418..7669
                     /locus_tag="VCF75_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546606.1"
                     /translation="MGRPLLTDDIIEKARRMETFESDDAVDFDTKVMTLPEKDEKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            7689..8384
                     /locus_tag="VCF75_001036"
     CDS             7689..8384
                     /locus_tag="VCF75_001036"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEP2546607.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQTLMTFLENLPV"
     gene            8535..8876
                     /locus_tag="VCF75_001037"
     CDS             8535..8876
                     /locus_tag="VCF75_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546608.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGGYRQGLTD
                     EKSKLQYSKDNAKLQLRNILNGYQSDLGKHYSSYYYYNLRTVMGLSSEKDIEEHYKKL
                     KNKLHDMYNHY"
     gene            complement(8975..9622)
                     /locus_tag="VCF75_001038"
     CDS             complement(8975..9622)
                     /locus_tag="VCF75_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEP2546609.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILNFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLNINTELTLTQIDTFAKTYTICYDDKDLVIPENIAKQLYVTAL"
     gene            9768..10700
                     /locus_tag="VCF75_001039"
     CDS             9768..10700
                     /locus_tag="VCF75_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEP2546610.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            10764..11489
                     /locus_tag="VCF75_001040"
     CDS             10764..11489
                     /locus_tag="VCF75_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP2546611.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            11490..12344
                     /locus_tag="VCF75_001041"
     CDS             11490..12344
                     /locus_tag="VCF75_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP2546612.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLGVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(12492..13298)
                     /locus_tag="VCF75_001042"
     CDS             complement(12492..13298)
                     /locus_tag="VCF75_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP2546613.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPEQDITITSIDIVKDYRFKN"
     gene            13515..15920
                     /locus_tag="VCF75_001043"
     CDS             13515..15920
                     /locus_tag="VCF75_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP2546614.1"
                     /translation="MVKRNQRKKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMPKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDRPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKIGVRNIA
                     GYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRMAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGSAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(15990..16343)
                     /locus_tag="VCF75_001044"
     CDS             complement(15990..16343)
                     /locus_tag="VCF75_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEP2546615.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTSLFKLNKKGFKFPDIALLLFA
                     VEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
BASE COUNT         5068 a         2794 c         3599 g         5006 t
ORIGIN      
        1 agagcgaaaa agggataatg gtaattagat attctgatag atatatattt acaaatggcg
       61 ggacacttag aatcgaccta aaggactttt ttagtggggc acattctgat ccaagaaact
      121 acttaataca agaaattttt aggtttctaa atttatgtga aaaagcggga actggtatac
      181 ctaaaataat ggaagcagtt aaagagagtc gtttaaaata tcccaaatta cgaactcaat
      241 tagactcagt tgaacttaca ttatgggata cttcccttat agataaccta tatattgata
      301 atgaatatga gaaaaaaata ttagagttaa taatcgaaaa ccgattcata acaagagaga
      361 ctttggaaga aaaactaaaa gtccataaaa atacaatttt aaaatattta aaaaaattag
      421 tggaaaaaca agctatcgat aaattcaaat ctggtagaca atacgtatac tttattgcac
      481 aaaaccaaga tcctgaattt caaaaatata accacattga tgccatgtat tctctactgg
      541 aagaaatgaa acgcaaataa tggtcttctc gtaagcttaa gcatgatata aatccaatct
      601 aacaccgttt tgataggagc ttgcctgctg atgctgatgg aaaggtttat gatcatgaaa
      661 ataacagaag ttaaaaagaa agatggtgct attgtctacc gtgctagtgt ttgccttggt
      721 attgatcagg taacaggtaa gaaagtaaaa atcagtgtaa cgggtaagac gcaaacagag
      781 gttaagcaga aagccaaaca cgcgcagttt gattttatat ttaatggatc tacaatatgt
      841 aaagaagcac agattaggaa ttatcaggag ttggcagaac tatggctaaa gagctatcag
      901 ctcactgtta agccacagac atatgtagcg actaagagaa tgctttacaa tcatttaata
      961 cctgtttttg gatctatgaa aattgataaa ttaactgtca gctatatcca acactttata
     1021 aatgacttat caagtcagtc agaccattac ggtgtagtgc attcgacaaa tagacgtgtt
     1081 ttacagtatg gtgtatctct ccaacttatt ccaaagaact acagtaggtt attaaaacta
     1141 ttaggtacac caaagagtaa agcaagaatt agggcaatca gtatcgacaa gaaaacagtt
     1201 aatatgttaa agctttacaa gaagagacag cgacaactgt ttttagaagt cggcggttgt
     1261 tctcctaagg tggtttttgc tactccaacc aaagaatatc aaaacatggc gacaagccag
     1321 gagtgtttaa acagatgatg caaggaaata gatattcctc gctttacctc tcacgctttt
     1381 agacatactc atgctagttt attggtaaat gctggtatca gctataaaga attacaatac
     1441 agatcaggtc atgccacttt ggctatgact atggatagtt atagtcactt atcaaaagac
     1501 aaagaaaaag aaccagtatc ttattatgaa aaagcaataa atagcttcta ggtgaactaa
     1561 aaggcgacca aatgtttaaa attacatttt ataacattca caaaatctat tatatcaacg
     1621 gatatagtca ataaaggaga acaatatgac atttgaagaa attctaccag gtttaaaagc
     1681 caagaaaaaa tatgtacgta caggttgggg tggcgctgaa aattatgtgc agttgtttga
     1741 tacccttgaa gtggatggga aagtactaca ggcgacgcct tattttctga ttcatgttac
     1801 tggtgcaggt gaaggttttt ccatgtgggc accgacacca tgtgatgtgt tagcagagga
     1861 ttggatcgag gtcaatgact aaagttgttt tagtgacggg ctgtgctagt gggattggtt
     1921 atgcacaggc ccgctatttt ttgaaacaag ggtatcacgt atatggtgtg gataagtcgg
     1981 ataagccgga cttaaacggt aattttcact ttatcaaatt ggatttatcc tctgagttat
     2041 ccccactatt taaagtggtc cccagtgttg atattttgtg taataccgca gggattttag
     2101 atgcctacaa gccactttta gacgtcagtg atgaagaagt ggaacaccta tttgatatta
     2161 atttttttgc gacggtgaag ttaacacgtc attatcttag acgtatggtg gaaaagcagt
     2221 caggtgttat catcaatatg tgctcaatag caagttttat agcgggtggc ggtggtgttg
     2281 cctatacgag ttctaagcat gcccttgcgg gattcacgcg ccagctggct ttggattacg
     2341 ccaaagatca gattcacatc tttggtatag cgccaggtgc tgtcaaaact gctatgacgg
     2401 ctagcgactt tgagcctggt ggcttagcag aatgggtagc tcgtgagacg ccaattgggc
     2461 gttggactaa accaagtgaa gttgctgaat tgacgggatt tcttgcttca ggaaaagcga
     2521 gatcaatgca aggtgaaatt gtcaaaattg acggtggctg gactttgaaa tgatcacatg
     2581 ccttagcttg agctggcgca gtaagcccta gaggtgtgac gagtcaaaca gtcaaaggag
     2641 atgaacaaat gaaattagcg gttttgggga caggtatgat tgttaaggaa gttttgcctg
     2701 tcttgcagaa aattgatggt attgatttag ttgctatctt atcaacagtt agaagtttaa
     2761 agacagctaa agacctcgct aaggcatatc acatgcctct agttacgagt aaatatgagg
     2821 ctatcttagg caatgaggag attgacacag tttatattgg attgccgaat cacctgcatt
     2881 ttgcctatgc taaggaggct cttttggctg gtaaacatgt catttgtgaa aagcccttta
     2941 ccatgacagc aggagaatta gatgaactgg tggtgatcgc taaaaaacga aaactcattt
     3001 tgttagaagc gattacgaat cagtatttaa gtaacatgac ttttatcaaa gagcatcttg
     3061 accaacttgg cgatatcaaa attgtagaat gcaattattc acaatattct tcacgctacg
     3121 atgccttcaa acgaggtgat atcgcgccag cttttaatcc taaaatgggt ggtggggctt
     3181 tacgtgattt aaacatctac aacattcatt ttgtggtagg attatttggt cgccctaaaa
     3241 cggttcaata tttagccaat gtcgaaaaag gcattgacac atcaggcatg cttgtcatgg
     3301 actatgagca gtttaaagtg gtgtgtattg gggccaaaga ctgcactgcg gaaatcaaaa
     3361 caaccatcca aggtaataaa gggtcactgg cagtgcttgg agcaacaaat acccttcctc
     3421 aggttcagtt aagtctgtac ggtcacgaag cacaagtggt taatcacaac aaacatgacc
     3481 accggatgta tgaggagttt gtcgctttta aagacatgat tgaccaaaaa gactttgaga
     3541 aagtgaatca ggctttggag catagccgag ctgtcatggc tgtgttggag cgtgctgttc
     3601 attcgtgact gtaatctggt agccattgcc ttttgtgcag ttgctatggt aatggttgaa
     3661 agacgtgttt tcacgagggt taaccccctc gtgctttttt ggcaaaaata acttgttcag
     3721 gatttgggcc ttctttgtga gatgagagat gcctgacgaa ttgatttgat taaaataatg
     3781 ataaagccaa acgatttaag aaatgaccgt tcggaaattc tgccccaaaa ggtctctaga
     3841 agcagtgaaa tcgaatttgt aagcgttgac actactgcat aactctgtta cactaagaga
     3901 atgtcatgac aaaaattaca gtgtaggagt ttaattgttt atggaagagg attataacaa
     3961 aagagaacct gagaagttta cgcaatttct gcgccgtcaa aaggttgtct tttttgtcgc
     4021 attttttggt tatgtgtgtg cttacttggt acgcaacaat ttcaaactaa tgtcaaacac
     4081 tattatggtg caaaatggtt gggacaaggc tcaaattgcg accttgttat cgtgtttgac
     4141 cgtttcttat ggcttagcaa aattctatat gggagcctta ggggatcgtg ttagcttgag
     4201 aaagctcttt tcgatcagct tgggtgcaag cgctttgatt tgtattttaa tcggtttttt
     4261 caatagttca atggtggtgt tagggatact gctagtcttg tgtggtgtcg tacaaggtgc
     4321 cttggcacct gcctcacaag ccatgattgc caattatttt ccaaataaaa cacgcggcgg
     4381 agccatcgct ggatggaaca tttctcaaaa catgggatca gccctcttgc cattaaccat
     4441 cgccttgctc accagtatgg gcttggtggt accagctaat ggcaatattt tacttgcctt
     4501 tttaatccct ggcattttag tgttcttgtt tgctttgtgt tgctggaagc ttggcggtga
     4561 taaccctgag tctgaaggac ttgactctct tcgaacgatg tatggtgatg cgggcgagtc
     4621 agctgttgcc agtgaagaag aaaagcataa cctgtcttat tggcaactca tctggaaata
     4681 cgttttttgt aacccgtcgc tcttacttgt tgctgctgtc aatgtagccc tttattttgt
     4741 tcgttttggg attgaagact ggatgccgat ctacttgtca caagtagcca atatgtcaga
     4801 ggctcatatc cattttgcga tttcaatatt agagtgggta gctattccag gctcgctggt
     4861 atttgcgtgg ttagcggttc gttatcctaa taaaatggcc aagattgggg ctattggact
     4921 ttttgtgttg gcggctattg tgtttgtcta tgaacgcttg actgccacag gtgctccaaa
     4981 ctatttcttg ttgcttgtta ttgcaggtat tttagggtca ttgatttatg gcccacagtt
     5041 gatcgtgaat attttaacaa tcaactttgt tcctttaaat gttgcaggaa cagcgattgg
     5101 ttttgtagga gtaacagctt atctcatcgg caatatggga gcaaactggc tgatgccgat
     5161 tttggcagat ggttttggct ggttttggtc atatattgtc gttgcagcct tatctgcttt
     5221 ttcagcggtt ggttatttga ttttagccaa acgtgaggaa gaaatcatca aagattagct
     5281 aggcaaatgc ctaagaaaga cagacttatt gctgaagtac tgtctttttt agtcatcaca
     5341 agccgcaaat gacactataa aagataagaa aatgctagga tgtttcagga aaaaaagaga
     5401 aaatatgcta taatgagaag gtatttaaat tatatagtaa ggaaaaacaa atgacaaatt
     5461 atgccattat tttagcagcg ggcaaaggga ctcgcatgac ttctgatctc ccaaaagttc
     5521 ttcataaagt atctggacta accatgcttg agcatgtttt tcgtagtgta aaagcgatta
     5581 gtcctgaaaa atccgtgacg gttattggtc acaaatcaga aatggtacgt gctgtcttag
     5641 cagatcaatc agcctttgtc catcaaacag agcagttagg aactggccat gctgtgatga
     5701 tggcagaaac acaactagaa ggactagaag ggcatacctt ggtgattgca ggagatactc
     5761 ctttgatcac tggagaaagc ctgaaaagtc tgatcgattt tcatgtgaat cataaaaatg
     5821 tcgccactat tttaacagct acggctcaag atccatttgg ttatggtcgt attgttcgca
     5881 acaaagatgg cgaagtgatc aagattgttg agcaaaaaga cgccaatgag tatgagcaac
     5941 aattaaaaga aatcaataca ggaacctatg tgtttgataa caagcgtctc tttgaagcgc
     6001 tcaaatgcat cactaccaac aatgctcaag gagagtatta cctgactgat gttgtggcta
     6061 tttttagagc aaacaaagaa aaggtaggag cctatatcct gagagacttc aatgaaagtc
     6121 ttggtgttaa tgatcgtgtg gccttagcaa cagctgaaac agtgatgcgc caacgcatca
     6181 cccaaaaaca tatggtcaat ggggtgactt tccaaaatcc tgaaaccgtt tatattgaaa
     6241 gcgatgttga gattgctcca gacgtcctta tcgaaggaaa tgtgaccttg aaagggcgta
     6301 cacatatcgg ctcaggaact gtcttgacga atggtactta tattgtggat tctgaaattg
     6361 gtcaaggaag tatcatcacc aactcaatga tcgaatcatc tgttttagct gcaggagtta
     6421 cggtaggccc ttacgcccac cttcgaccag gaacgacctt agatagggaa gtccatatcg
     6481 gtaactttgt tgaggtcaag ggttctcaca ttggtgaaaa aacaaaggct gggcatctga
     6541 cttatattgg aaacgctcag gttgggtctt cagtcaatgt tggggctgga accatcactg
     6601 tcaactacga cggccaaaac aaatatgaaa cagtcattgg agatcacgct tttattggga
     6661 gcaactcgac tctcattgca cctttggaag ttggagataa tgctttaact gcagctggct
     6721 caacgatttc taagacagta ccagctgata gcattgttat tggtcgtagc cgtcaagtca
     6781 caaaagaagg ttatgccaag cgcctagcgc atcacccaag tcgaagcaaa taattcaaga
     6841 tctatttagt aaggaggcca ggcatgaaat ttgaagaaaa aacgcttaag agacaaacag
     6901 tttttgatgg tcatattttt aaagttgttg tggatgatgt tgaacttccc aacaatctgg
     6961 ggcaatccaa gcgagaatta atttttcatc gtggtgccgt tgctgttttg gctattactc
     7021 ctgaacgtaa gattgtcctt gtcaaacaat accgtaaggc tattgaaagt gtctcatacg
     7081 agatcccagc gggtaaacta gagattggtg aggaaggctc aaagctaaaa gctgctgcac
     7141 gtgagctaga agaagaaaca gcctatacgg ggacattgac gtttttgtat gaattttata
     7201 cagcgattgg tttttgcaac gaaaaaatca cacttttctt agcgacagac ctaacacaaa
     7261 ttgccaatcc caaaccacaa gacgacgatg aagttatcga ggtcttagag ttgacatatc
     7321 aagagtgcat ggacttagtc gctcaaggta aacttgctga cgctaaaacg ttgattgctt
     7381 tgcaatacta tgccttacat tttggaggtg agcagtgatg ggaaggccat tgttgaccga
     7441 tgatatcatc gaaaaagcga gacgaatgga aacatttgag tcagatgatg ctgtagactt
     7501 tgacacaaaa gtaatgacgc taccagaaaa agatgaaaaa gcccgcattt ataaaagccg
     7561 tcggattgaa aatgccaaac gcagccaatt gcaatctaaa ttaaatgtga ttttgatagc
     7621 tgttatgctg ttaattgcca tcttggtata tgctatcttt tatctttaaa aaaacgtaag
     7681 gaaatgatat gaaaatagga attattgctg ctatggagga ggagttgtcc ctgctcttag
     7741 caaatctttt ggatgctcaa gaacatcagg tgctttcaaa aacttactat acgggtcgtt
     7801 ttggcaagca tgagctcatt ttggtacagt caggtgttgg aaaagtgatg tctgctatga
     7861 cagttgcgat cttggttgag catttcaaag cccaagctat tatcaataca ggatcagcag
     7921 gggcagtcgc ttcacattta gcgatcggag atgtggtggt tgcagatcgg ttagtgtatc
     7981 atgatgtgga tgctactgca tttggttatg cttatggtca aatggctggt caaccattat
     8041 actatgattg tgatccacag tttgtggcta tcttcaaaca agtcctaaaa cacgaaaaga
     8101 caaatggcca agttggtttg attgcaacag gagacagttt tgttgctggt caggacaaga
     8161 ttgaccagat caaaacagct tttagcgacg tgttagcagt agaaatggaa ggcgcagcta
     8221 ttgctcaagc agcacatact gcaggtaaac ctttcatcgt tgttcgtgcg atgagtgata
     8281 ccgcagcgca tgatgccaat attacttttg atcaatttat tatagaagct ggcaagcgtt
     8341 cggcccagac attaatgaca tttttagaaa acttacctgt ttaactcacc ttatcgtgag
     8401 acaattagct ggataatctc tattttagga gtctggtcac ctcttgtttt gaaacctaaa
     8461 aataagaatt gcaatcactt gaccaaacgc tataatgggt taaggaacga tagattacta
     8521 aaaggagtct aaaaatgaca ctaaaaaaac actattatct tctcagtctg ctagctcttg
     8581 taacggttgg tgctgccttt aacacaagcc agagtgtcag tgcacaagtt tatagcaatg
     8641 gaggatatcg tcaaggtttg actgatgaaa aatcaaagct gcaatatagt aaagacaatg
     8701 ccaaacttca attgagaaat atccttaacg gctaccaaag tgacctaggg aaacactact
     8761 ctagctatta ttactacaac ctaagaaccg ttatgggact atcaagtgag aaagacattg
     8821 aagaacacta taaaaagctt aagaacaagc tacatgatat gtacaatcat tattaagtca
     8881 aaggcttaaa aatggcgact agcccctcaa aaaaagaggc cacactcatt taaatttaaa
     8941 tgagtgtggc ctctttttga ggagacgcaa ggggttaaag agctgtgaca tagagttgct
     9001 tagcaatatt ttcagggatg acaaggtcct tgtcgtcata acaaatcgta taggtttttg
     9061 caaaggtatc aatttgagtt aaggttagct cggtattgat attgaggtga tgtgcttcta
     9121 gatactggat taaatcaaag tggtcgtgga ttctgcttag gcgaaaccgt cccaactccg
     9181 tgatggtgtt gagagttgta gtgttcattt cgacaagagg ctgaccatag cgaggaatag
     9241 tccctccgtg tgggcagaaa tctggaaaat taaggatctt gtctaaacgg tcgataaagg
     9301 tgtctgagac agtgtgctct aagacttcag cttcttggtg cacttcttgg gtattatagc
     9361 ccagctggtg aatcaaaaat acctcaatca agcggtgttt gcggtagagg ttagcaacaa
     9421 gagcatagcc tttgtctttt agcaaatagc cttttgcttt atctttgaca atccagcctt
     9481 gtgaaatcat ttttttaatc atctctgaaa cggcaggtgc cgaaacgtgc atcttttctg
     9541 caaccatttt atttgtaatc ttgggttctt gttctccaat ttcataaata catttcaagt
     9601 aatcttcttt attaggcgtc atatcgtttg gcctttctct tatttataat gattattata
     9661 tcagaatttt taggaaaaag cttgacaaaa agcctaaaag aggatatcct tatagcataa
     9721 ttaactaaac ttaattaaat aaataatata tcctaaggag agaaatgatg ggaaagagaa
     9781 tgagcctcat tttgggtgct tttttatctg tttttctttt agtagcttgt tcgtcaactg
     9841 gcactaaaac tgctaagagt gataaattaa aagtcgtggc aaccaattca attattgccg
     9901 acatgacaaa agctattgct ggtgataaaa tcgatctgca cagcattgtg ccaatcggtc
     9961 aagatcctca tgagtacgaa ccattaccag aagatgttga aaaaacaagt aatgctgatg
    10021 tgattttcta taatggtatc aatctagaag atggtgggca agcttggttc accaaactag
    10081 tgaaaaatgc tcaaaaaacg aaaaacaaag attactttgc cgtgtctgat ggcattgatg
    10141 tgatttactt ggaaggtgca agcgaaaaag gaaaagaaga tccacatgct tggttaaatc
    10201 tcgaaaacgg aatcatttat tcaaaaaaca ttgccaaaca attgattgca aaggatccta
    10261 aaaacaaaga aacttatgaa aagaacctaa aagcttatgt ggctaaattg gaaaaactag
    10321 acaaagaagc caaatcaaaa tttgatgcta ttgcagaaaa taaaaaattg attgtgacta
    10381 gtgaaggctg cttcaagtac ttttcaaaag cttacggtgt cccatctgct tatatctggg
    10441 aaattaacac cgaagaagaa ggaacaccag atcaaatttc atcattgatt gaaaaactaa
    10501 aagtcatcaa gccatctgcg ctttttgtag agtcaagtgt cgatagacgc cctatggaaa
    10561 ctgtttctaa agatagtggt attcctattt attctgagat ctttacagat tcaattgcta
    10621 aaaaaggtaa acctggcgat agttattatg ctatgatgaa atggaacctt gacaaaattt
    10681 ctgaaggtct agcaaaataa gataacaata aagcacagcg attttagtgt tgtgctttgt
    10741 tttatttatg taaaggagac aaaatgatta caactaacaa tctttgtgtg acctacgatg
    10801 gcaacagtaa tgctttagaa gcgattaatg tgacaattga aggtccttct atcgttggta
    10861 ttatcggtcc taatggagct ggaaaatcaa cctttatgaa agctatctta aatcttattg
    10921 attatcaagg tcacgtaacg gttgatggca aagatggtcg taagcttggg catacagtag
    10981 cttacgtaga acaacgaagc atgattgact ataattttcc tattactgtt aaagaatgtg
    11041 tggcgctagg gacttatagt aaattaggcc tttttcgccg agtaggaaaa aaacaatttg
    11101 agcaggttga taaggttctc aaacaagttg gtttagaaga ctttggccat cgaccgataa
    11161 aatccttatc tggtggtcag tttcagcgga tgttggttgc gcgttgcttg attcaagaat
    11221 cagactatat tttcttagat gaaccgtttg ttggcattga ctcggtcagc gaaaaaatca
    11281 ttgtagatct tttgaaagag ctcaaaatgg ctggcaaaac gattttaatt gtacatcatg
    11341 atttgagcaa agtagagcat tattttgaca aactcatgat tttaaataaa catttggtgg
    11401 cttatggcaa cgtttgtgag gtatttactg ttgatacctt gtcaaaagct tatggcaatc
    11461 atttgatttt aggaaaggag atggtgtaaa tgtctatgaa attttttgag ggcttgatgt
    11521 cataccactt tttacaaaat gcactgataa cggctgtagt cattggtatc gtctcaggtg
    11581 ctgtagggtg ttttattatc cttaggtcaa tgtctcttat gggtgatgcc atctcacacg
    11641 ctgttttacc aggggttgct ttgtcattta ttttaggagt caattttttt attggagcga
    11701 ttatttttgg gttattagct tctgttatta ttacttatat caaggaaaac tctgttatta
    11761 aaggagatac ggctattggt atcaccttta gctctttttt ggcactggga gtcatcttga
    11821 taggggtagc caatagttcg acggacttat ttcatatttt gtttgggaat attttagctg
    11881 tgcaagatag cgataagtgg attactattg gtgtttcgat ttttgtttta ggggttatta
    11941 gtctcttttt caaagaacta ttattaacat catttgatcc tatcttagcc aaatcaatgg
    12001 gtgtgaaggt caatgcgtat cattatttgt tgatggttct attaacttta gtggctgtta
    12061 cggcgatgca aagcgtgggt actatcttga ttgttgcctt actgattacg ccagcagcga
    12121 cagcttattt atatgctaat agcttaaaag taatgttagt gatgtcatct ttactaggtg
    12181 ctttagcatc ggttttaggg ctttatttgg gctatacctt taatgttgcc gcagggtcaa
    12241 gtatcgtact gacttctgcg atgatgtttt tgatcagttt ctttgtttca cccaagcaag
    12301 gctacctcaa aagatggatg caaaaaaaag aaaaaacacc ttaaactttt cgctttaaac
    12361 accttagtgg tttgcaaata gtggcaaatg acatcaaagg gtgtttttta tatgtcatca
    12421 aaagaccata aaaaacctta tcaaaccaag gtatttagcc tattatctga taaggttttt
    12481 ttaagcatga tttagttttt aaagcgataa tctttgacaa tgtcaattga ggtaatcgta
    12541 atgtcttgtt ctggtttatc attttggttg atagaagtcg cggcaatttt atcgacaaca
    12601 tccataccat caatgacttg accaaataca gtataaccgc catctaagct tggattgccg
    12661 ccatgctcat aggcagagat gataggtttt gggtagttgg tacttgataa tcccttgctt
    12721 tgatttttct tgttttggtt gatataaaat tggctaccgt tcgtattagc gccagcattt
    12781 gccatggcaa gagcaccgcg aatatgatat aaaaatggag agatttcgtt gacaaagcca
    12841 ttgccagcat cttttttagg atccttgccc ttccaaatcg attcaccacc tgtgccatct
    12901 cctttggggt cacctgattg aatcataaag tcgttgatca cacgatggaa ggtaaggtta
    12961 tcatagtagc cttttttagc atgggtgagg aagttctcaa cagctaaggg agcatatttt
    13021 gggaataact taagggtgat gtccccttga cttgtcctca ttacgacctt agcttcttct
    13081 ttgccgactt ctttggttag ttgtggaaat tggctagcat cagctttgag agctttttga
    13141 atgctttctt cataggcttt agaagctgct tcgctttctt ctttggcagt tttttcatca
    13201 atgtatttat cgccttttat agcgcggtca acagattcac aagcactcaa actaagtaaa
    13261 ctaatggcta ctaaactgag agataataat tttttcatgt ctactcctaa gaatgatttt
    13321 ctttatttta tcataaaatg caacagaaaa tagtagtaaa gagttaaaaa cgcctatttt
    13381 tgtgttttat gatctaacct tggttaaaag gctgctacga ctgttattat agcacaagat
    13441 gctatagaaa aacttaaaac accttggttt tttagagcta tttcctaata gttaaaaaat
    13501 ggtataatag tgttatggtt aaaagaaatc aaagaaaaaa aagtgcgcct aagaagcgtt
    13561 taacaaaagc agaagtggaa aagcaacgtg ccattaagag gatgatttta tctgttttaa
    13621 tggctttgtt gcttattttt gcgatgcttc gcttgggcgt ctttggcgtg acaacttata
    13681 atatgattcg ttttttggtg ggtagtttag cctatccgtt tatgtttgcc tggttaattt
    13741 acttattttg ttttaaatgg ttacggcaaa aagatggaat gattgcggga gttgttattg
    13801 cctttttggg attgctagtt gagtggcacg ccttcttatt tgcgatgcct aggatgcttg
    13861 atcaagatat ttttctaggc actgctcggc taattacaag agatttacta gccctgcgcg
    13921 tgactgaatt tgttggtgga ggaatgcttg gtgcattact ttataaaccc atagcctttt
    13981 tattttcgaa tatcggctct tactttattg gttttttgtt tatcttatta ggattatttc
    14041 taatgacgcc ttgggatatc tatgatgtga gtcactttgt gaaagaggct gttgataaac
    14101 tagcagtggc ttatcaggaa aataaagaaa agcggttcat caaacgagaa gagcaccgct
    14161 tacaagctga aaaagaggct ttagagaaac aagcacaaga ggaagaaaaa cgtctagcag
    14221 aattgacggt tgatcctgag acgggagaga ttgttgagga tagtcagtct caagtcagct
    14281 atgatttggc tgaggatatg ccaaaagagc cagagattct tgcttatgat agtcacctca
    14341 aagacgatga ggcaagctta tttgaccaag aagacttggc ttacgctcat gaggaaattg
    14401 gggcttatga ctctttatct gctttagcat cttctgaaga tgagatggat atggatgaac
    14461 ctgttgaagt tgatttcaca ccaaaaaccc atcttcttta taaacttcca acgatagatt
    14521 tatttgcacc agataggccc aaaaaccagt ccaaagagaa aaacttagtc cgcaaaaata
    14581 tcaaggtgct tgaagatacc ttccaaagtt ttgggattga tgtcaaggta gaacgtgctg
    14641 aaattggacc atcagttact aaatatgaaa tcaagccagc tgtcggggtc cgagttaatc
    14701 gtatttcaaa tcttgctgat gatttggcac ttgctctggc tgctaaagat gttcgtattg
    14761 aagcgccaat tcctggaaaa tccttaatag gtattgaagt tcctaactca gaaattgcaa
    14821 cggtttcttt ccgcgaactt tgggaacaat ctgatgccaa tcctgaaaac cttttagaag
    14881 taccactagg taaagctgtt aatggcaacg ctcgcagttt taacttagct agaatgccgc
    14941 atcttttggt agctggttca actggttcag gtaaatctgt ggcagttaat ggcattattt
    15001 caagtatttt gatgaaggca cgtccagatc aagttaagtt tatgatgatt gaccccaaaa
    15061 tggttgaatt atctgtttat aatgatattc cacatctatt aatcccagtt gtaaccaacc
    15121 cgcgtaaagc cagtaaggca ctccaaaaag tggttgatga gatggaaaac cgctacgagt
    15181 tatttagcaa aattggtgtg cgtaatatag caggttataa tacaaaggtt gaagagttta
    15241 atgcttcttc tgagcaaaaa caaataccgt tgcctttaat cgttgtcatt gtagatgaat
    15301 tggctgactt gatgatggtg gctagtaaag aagttgaaga tgctattatt cgtttgggac
    15361 aaaaagcacg tgctgcaggt atccatatga ttcttgcaac tcaacgtccc tctgtagatg
    15421 ttatttctgg tttgattaaa gcaaatgttc cgtcgcgtat ggcatttgct gtttcaagtg
    15481 gtactgatag ccgtacaatc cttgatgaaa atggtgctga aaagctcttg ggacggggtg
    15541 atatgctctt taagccgatt gatgaaaatc accctgttcg cttgcaaggg tctttcatct
    15601 cagatgatga tgtggagcgt attgtcaact ttatcaaaga ccaagctgaa gcggattatg
    15661 atgacgcctt tgatcctggt gaagttagcg acaatgatcc tggtttttct ggaaatggcg
    15721 gatctgctga aggtgatcct ctttttgaag aagcaaaagc cttggtctta gaaacacaaa
    15781 aagctagtgc ctcaatgatt caaaggcgtt tgtcggttgg atttaatcgt gccacgcgct
    15841 tgatggatga attggaagaa gcaggtgtta tcggtccagc agaaggaacc aaacctcgta
    15901 aggtattgca aacaaactaa aagaacctga cgtctcatga tgagatgtca ggttcttttt
    15961 ttgtgatgtc aggccagttg atgagatgcc tatgctaatg taaaaataaa gataactaga
    16021 ataaggtaga aaataacggt gactaaaaag ccaactcgcc aaaatagctt cataaagcga
    16081 tggtaagaaa acgactgagt tcggatgact agcagtagtg aaatgacgat ggctagcagc
    16141 gacatgataa taaggtaata aggcagtaaa ttatgggtga aaaattttcc tgagacaagc
    16201 acaatttcta ccgcaaaaag taaaagtgcg atatctggaa atttaaatcc ttttttatta
    16261 agtttaaata aagaggttac aatcatggca aaaagaggcg ttactatcaa gaaagctaag
    16321 gcagttaatt tatagatcat cataggtcta ttttatactc aaatccctta cttgtaaact
    16381 gatacttacc tgttgtgata gaaaatgatc tttgagaggg aaagcgccta cttcaattta
    16441 aacattgcaa aaggcatcag ctatttt
//
LOCUS       DATURV010000032        16053 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DATURV010000032 DATURV010000000
VERSION     DATURV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16053
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.032"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(11..727)
                     /locus_tag="VCF75_001045"
                     /pseudo
     CDS             complement(11..727)
                     /locus_tag="VCF75_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
     gene            689..2161
                     /locus_tag="VCF75_001046"
     CDS             689..2161
                     /locus_tag="VCF75_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2546616.1"
                     /translation="MLIVMENRNMTFLSIKNQETHLLTSTGKMLASHQAIKELLLNNQ
                     PNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPNPKNIGKPFQGGDEEAVLAGKKVI
                     STAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTTLNDVALTSKRNYTLSLLLCLLIS
                     LLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLDQIEAAVFVVDKAGILQLCNQAGQ
                     KLIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQLFRYGEEDYLLAISPICVKNDHR
                     GHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHKFMNQLHVIYGLVDIAYYDQLKIY
                     LDSILEPENEILTSLSVLVKEPLLASFLIGEQEKYQELNVHLKIDVLSEIPHSATKNQ
                     LNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHLISHYTLTDNWIDLERVQPIFDLP
                     YFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGGQHERFNH"
     gene            2142..2807
                     /locus_tag="VCF75_001047"
     CDS             2142..2807
                     /locus_tag="VCF75_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEP2546617.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRTQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(2862..3935)
                     /locus_tag="VCF75_001048"
     CDS             complement(2862..3935)
                     /locus_tag="VCF75_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP2546618.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKVPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDGIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(3928..4704)
                     /locus_tag="VCF75_001049"
     CDS             complement(3928..4704)
                     /locus_tag="VCF75_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546619.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            complement(4701..5495)
                     /locus_tag="VCF75_001050"
     CDS             complement(4701..5495)
                     /locus_tag="VCF75_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546620.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            complement(5479..6633)
                     /locus_tag="VCF75_001051"
     CDS             complement(5479..6633)
                     /locus_tag="VCF75_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546621.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            complement(6679..7566)
                     /gene="murB"
                     /locus_tag="VCF75_001052"
     CDS             complement(6679..7566)
                     /gene="murB"
                     /locus_tag="VCF75_001052"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEP2546622.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     VYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSVGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            complement(7716..8216)
                     /gene="folK"
                     /locus_tag="VCF75_001053"
     CDS             complement(7716..8216)
                     /gene="folK"
                     /locus_tag="VCF75_001053"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEP2546623.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKLLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            complement(8213..8572)
                     /gene="folB"
                     /locus_tag="VCF75_001054"
     CDS             complement(8213..8572)
                     /gene="folB"
                     /locus_tag="VCF75_001054"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEP2546624.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGGKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            complement(8579..9379)
                     /gene="folP"
                     /locus_tag="VCF75_001055"
     CDS             complement(8579..9379)
                     /gene="folP"
                     /locus_tag="VCF75_001055"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEP2546625.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESARPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWIDPGFGFAKSVQQNTELLKGLDRVCQLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            complement(9388..9972)
                     /gene="folE"
                     /locus_tag="VCF75_001056"
     CDS             complement(9388..9972)
                     /gene="folE"
                     /locus_tag="VCF75_001056"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEP2546626.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEHHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKQPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            complement(10007..11284)
                     /locus_tag="VCF75_001057"
     CDS             complement(10007..11284)
                     /locus_tag="VCF75_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP2546627.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLQDRYP
                     RLTPDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIVDMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRRYWKKHIEKSVLLTH"
     gene            11616..12578
                     /locus_tag="VCF75_001058"
     CDS             11616..12578
                     /locus_tag="VCF75_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP2546628.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            complement(12694..13929)
                     /locus_tag="VCF75_001059"
     CDS             complement(12694..13929)
                     /locus_tag="VCF75_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP2546629.1"
                     /translation="MIKRWAILFFVVLTCSGLSKIVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDRVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVADSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVDRTYCRRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(14087..15274)
                     /locus_tag="VCF75_001060"
                     /pseudo
     CDS             complement(14087..15274)
                     /locus_tag="VCF75_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106750.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15277..15498)
                     /locus_tag="VCF75_001061"
     CDS             complement(15277..15498)
                     /locus_tag="VCF75_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEP2546630.1"
                     /translation="MNIGFLEHTARNIIREAKRIAIKKFEEAREIDQNAVQLSKSPFD
                     NRRLGIAPKDIVEELVGILLSEESLESEN"
     gene            complement(15799..16004)
                     /locus_tag="VCF75_001062"
                     /pseudo
     CDS             complement(15799..16004)
                     /locus_tag="VCF75_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         5019 a         3464 c         2697 g         4873 t
ORIGIN      
        1 ttcatatatt ctatgattta aggactaccg acctgacgct tttagcgaca atctcagcaa
       61 ctccttcttt aaaggcatct ggaataatat tagtagtgct caaagcgtca cctggaacaa
      121 gactggcaat accttttgcg gcagcaattt gcatctcaac agtaatggtt tttgcccgtg
      181 catctaatgc tcccctaaaa atacctggaa aggcaaggac attattaatc tggttaggaa
      241 aatcactacg gccagtacct acaatgtaag cccccgcttc aagggcttca tcagggtaaa
      301 tctctggaat agggttggcc atggcaaaaa ttactgggcg cgctgccatt ttactaatcc
      361 actctgcttt taagactcct ggtactgata cgccaatgaa gatatctgct ccttcaagag
      421 catcttctaa ggtacctgat ttaaactcac gattagtgac tttcgctata tccaaatggt
      481 gtggtgctaa ctgatctgcc tcttgttcat tgataatgcc aaatttatcc actactgtca
      541 ctttcgtagc acctgctgct aataatttac gggtaatgga taatcccgca gatccaccac
      601 cgttcaccac gatggatacc tcatcaaggg atttttgaag aagtttcaaa ctattaaaga
      661 tggctgctaa caccacaata gctgttccat gctgatcgtc atggaaaaca ggaatatgac
      721 attcctttcc atcaaaaatc aggaaaccca tttgctaacg tcaactggaa aaatgttagc
      781 aagtcatcaa gcaattaaag aattactcct aaacaaccaa ccaaatgcta aaacaacagc
      841 ctataccaac tccattgcct ccatctataa ccttgattat gtggtcgtga tgaacatgaa
      901 agggattcga ctcacccatc ctaacccgaa aaatatcgga aaaccttttc aaggaggtga
      961 tgaggaggct gttctagcag gaaaaaaggt tatctctacc gccaagggca ctttaggaaa
     1021 gtcacttcgc tatctagtac ctgtgtttga cggtgacaaa cagattggtg ctattgcggt
     1081 tggcattaaa ctaactaccc taaatgatgt ggctttaacg tcaaaacgca attataccct
     1141 gtctttactc ctttgtcttc ttattagttt actggtcacc agttttattt cttttcgact
     1201 caagcgtcaa ctgcaccagt tagaaccaag tgaaatttac cagctctttg aagagcgtaa
     1261 tgccatgttg gatcaaatcg aagccgccgt atttgtggta gataaagctg gtatcttaca
     1321 gctctgcaat caggctggac aaaaattaat tgctcggaaa tgccagttgg gaaaacctac
     1381 tggcaacagt ttcaatcacc tctttcctga ttttccaaaa cttagtttgc aagaggggca
     1441 tgaacaactc tttcggtatg gtgaggaaga ctacttgtta gccatttcac ccatctgtgt
     1501 caaaaacgat caccgaggcc acattatttt catgcgagaa gcagttaagg ctattgatac
     1561 cttggatcag ctagcctata ccactgccta tgcttctgct ctacaagccc aaactcataa
     1621 attcatgaat caattgcatg ttatttatgg cttagtggat attgcttatt atgaccaatt
     1681 gaaaatttat ttagacagta ttttggagcc cgaaaatgaa attctcacta gcttatctgt
     1741 ccttgttaag gagcctcttc tagctagttt tctcattgga gagcaagaga aataccaaga
     1801 attaaatgtc catctcaaga ttgatgtttt aagcgagatt cctcattctg ccaccaagaa
     1861 tcaacttaat aatggtttga tgatttaccg ctttatccat actaatcttc taaccacact
     1921 gagacccaaa tcactcgttt tgagtattca acatgatcag aatcatttaa tcagccatta
     1981 caccttgaca gacaattgga ttgatttgga gcgggtacag cctatttttg acttacctta
     2041 ttttcaacag ctcttgaccg atacaaattc ccaattccat caacatcaaa ccgtaaccga
     2101 gttacagttc tcagtgacaa caccttatgt aggagggcaa catgaacgtt ttaatcattg
     2161 aagatgatcc tatggtggat tttatccatc gcaattacct cgaaaaacta aatctttttg
     2221 atcgcattat atccagcgac tctatgaaag ctgttcaaag catacttact gattacgcaa
     2281 ttgacctcat tttactggat attcatatta cagatggcaa tgggattcaa tttttggaga
     2341 aatggcgtac tcagcatatt ccttgcgaag tcattattat ttcagccgca aatgatggga
     2401 atattatccg agatggtttt catttaggaa ttatcgacta cctcatcaag ccctttacct
     2461 ttgaacgctt tcaagaaagc atccaacagt ttgttacaca tcgagaacat ttggccaatc
     2521 agcaactaga gcaggcccaa attgaccaac tgaaatgttt gactagcaaa aaagacacta
     2581 aaaacaaaca attgttagaa aaaggcttat ctgaatccac tttccagtgg attatggaaa
     2641 atatcaaagc ctttgatcaa ccttttacta tccaagaatt agcttccgct tgtcacttat
     2701 cccatgtctc tgtccgtaaa tacattgctt atttagaaga aaataagcaa ctgaacagcc
     2761 aacaaatttt taccaaggtt ggccgtccct atcgcgtgta ctggtgacgt catctgtcga
     2821 tacattaaaa aggctggaga aataccagcc tttttgagtg cttatttgcg atacatttta
     2881 aattggaggt ataaatcatt ataaatcccc aaccatcttg accctaaatt atcataaact
     2941 tccaattttt tgataatgcc atctgttgga taaaaagcag gatcattttt tatctcatct
     3001 ggaagtaagg ccttggcttt tttatttggt gtcgcataac caatatatgc agcattttgc
     3061 gcagcatttt caggacgatt gataaagttc aaaaaagcat aagcttcttt ttcatgtttc
     3121 atggtttttg gtagtaccaa attatcaaac caaaggttag acccttctga aggcacgatg
     3181 tagtgaaggt gttcgttact atctaacatc tcactggctt caccagaaaa ggtaattcca
     3241 atagcagcgt caccttgaat catgtagcct ttcatctcat ctgctacaat ggctttaaca
     3301 ttcggcgtca actgttgcag ttttctctcg gctgcctgca actgctctag atttttagaa
     3361 ttcacactat aaccaaaagt tgttaaacca acccctagca tttcacgcgc tccatcaatc
     3421 agcataatac tatttttata ttctgggcgc cacagatctt cccagtgcat aggcacctta
     3481 tcaactaatt gatcattata aacaatccca acggttcccc agaaataagg caaagaatag
     3541 tcgttttgtg ggtcaaagct tttccctaaa aattctttcc cgatattatc catgccaact
     3601 aattttgact tatcaagctt attgagtagg ttttctttga tcattttatc aatggtgtaa
     3661 tcactaggaa cagcaatgtc gtaagtggtt ccgccctgct tgattttagt gtacatggct
     3721 tcattggaat cgaaagtttc atactgcact tcaatgcccg tttctttggt gaattttttg
     3781 agcaaagctg gatcaatgta atctccccag ttataaataa ctaatttatc cgattgacta
     3841 ccagaacccg attttttctg caagatgaaa gaaagacttg ttaaaataac aataacaccc
     3901 aaaactcctg ctagaaagga ataaagttta cgcatgtttt tcctccttgt cctgtgacat
     3961 ataataataa ccgatcacta agaggatgga gaaaaagaaa acgatggttg acaaagcatt
     4021 gatatccaag gaaattccct gacgagcccg cgaataaatc tcaacagata aagtagtaaa
     4081 accatttcca gtcaaaaaga aagtcactgc aaaatcatct aaggaatagg taaaggccat
     4141 aaaataacct gcaataatcc ctggtgttaa gtatggcagc atgacttctt tgagcatttg
     4201 gaaataatta gctcccaaat cataagcggc gttgaccatg tcttgattca tctctttcaa
     4261 gcgcggcaac accatcaaaa ccacaatagg aatcgaaaaa gcaatatgac ttaataaaac
     4321 tgaagacatg cccagctgaa acttcaatga tgtaaaaaga attaaaaagg aagccccaat
     4381 catgacatct ggtgatacca tcaagacatt attggctgat agcatggcat tttggtactt
     4441 acctctaaca tggtggataa agatagctcc aaaggtccca ataatcgttg ctagtagagc
     4501 gctactaaaa gcaaggacaa aggtttgcag taagattgtc atgagacgac tatcctcaaa
     4561 catggtttgg taatgctcaa gggtaaatcc tgtaaaacca ttcatatccc cacctttgtt
     4621 gaaagaatag aaaatcaaat aaaaaatagg aatgtagagt aaaacaaaga cactcgctaa
     4681 ataaagattg gcaaattttt tcatttactt ttctcctttg tgagccacat aatagcaacc
     4741 atggtcaaaa tcaagacgac accaatggtt gatcccattc cccagttttg ggtggtcaaa
     4801 aaatgttgtt caatggctgt accaagtgta atcacgcggt ttccaccaat caaacgggtt
     4861 aacataaaga gactcaaact tgggataaag acagactgaa cacctgccct aaccccattt
     4921 aaagaaaggg gaaaaataac ttttgagaag gcctgaaatt cactagctcc tagatcgcga
     4981 ctggcattga tgacattatg gtcaatatca tccaaagcat taaaaatcgg taacatcata
     5041 aaaggaagct caatgtaaga ggctacaaaa atgaaggaga aatccgtgaa aaggatttgc
     5101 tgcgggccaa tccccataaa agttaaaaag ctgttaatcc ctccttgttg accaaagatt
     5161 cccataaagg cataggcttt tagcaataag tttacccaag ttggcaaaat aatgagcata
     5221 agccacaact gcttgtgctt taggcgcgtt aaaaagagag ccgtaggata tgaaattaag
     5281 agcgtgacga gtgtgataat accagcgtat aaaatagaat tcacactcat tctcaaatag
     5341 gtccaagagc taaaaaaggt ttcataattg gctaaggtca cgcgcccttc tatgtcaaaa
     5401 aaggacttgt aaaacaagag agtgactggt gccacaacaa aaaagagaat ccataagaag
     5461 taaggaatcg aaaaaagact agaggttttc ttcattacgc tcctcctcaa tggcattaat
     5521 caatccatct tcaggctcat ccatttccac atattcttca atacgggcat caaattcctc
     5581 ttcagtctca ttaagacgca tgatatgaag atcttcaggg gtaaagtcta atccgataac
     5641 ttctccctcg atagctttgc gggtagaatg aatcatccat tcattaccca attcatcata
     5701 ggcaataatt tcgtagtgaa ccccgcggaa taattgggta tcaaccttaa cttgtaattt
     5761 cccttcttct ggcaaagtaa tttgaagatc ttcaggacga ataaccactt caacaggctc
     5821 attagggcgc attccaccgt ccacagattc aaattctttc ccgttaaagg agacaagata
     5881 gtcttcaatc atggtaccgt taataatatt agattctcca ataaaattag caacaaaatg
     5941 attaattggc tcatcgtaaa tatccactgg tgttcccgac tgaacaattt caccttcatt
     6001 catgacaaaa atccaatcac tcatggccaa ggcttcttct tgatcgtgag taacaaaaac
     6061 aaaggtaatg cctagacgtt gttgcaattc acgtaattca tattgcatct ctgttcttaa
     6121 tttcaaatca agggctgaga gtggttcgtc aagtaagacc acacgcggtt gattgataat
     6181 cgcacgcgca atggcaacac gttgacgttg accaccagat aacttctgga tagaacgatt
     6241 ttcataccct tctaattgaa ccattttcaa ggtttctttc acgcgcttag caatctcttt
     6301 cttgtccact ttcttcagct tcaaagcaaa ggcaacattc tcaaaaacag tcatatgagg
     6361 gaagagggca taattttgga aaacggtatg aatgtcacgt ttattaatcg gcaaatcatt
     6421 aatacgttcc ccatctaaat aaatgtctcc gctgctggca tctagtaggc ccgccataat
     6481 atttaaaata gttgattttc cagagccaga agctccaagc agggtgtaaa attttccttc
     6541 ttcaaggtca aagttaatgt tctttagaac ctgtgttcca ctgtcctcaa atgtttttga
     6601 aacattgtta aatgtgataa ttggcttagt caattgtcat aaatcctcca cggtactttc
     6661 tttaattatc aataatggtt acaggttttc cccaataatc cgaacttctg gttcaagcct
     6721 gacaccagaa tggttttcaa ctgtctcaat gacataagca atcaaatctt cataatcttt
     6781 agctgtgcca tctgccacat taatcataaa accagcgtgt ttttcggaaa cttccacacc
     6841 accaatccga tgccctttaa gatttgcttc catgattaat tggcctgcaa agtgtcctgg
     6901 cgggcgctta aacacagatc cacaagatgg aaattctaaa ggttgtttta gttggcgaag
     6961 gtgatttaac cgattcattt cttggctgat cgtatcataa ttacccggtt taagagcaaa
     7021 cttagcagaa atgacaatgt cacctgtttc ttgaatggca gagtgacggt aaccaaaagc
     7081 catatcccta gctgaaatgg ttttgatctc tccacttgag gtaagcacct tagcggataa
     7141 gaaaatatgt gatatttccc caccataggc acccgcattc ataaaaacag cacccccaac
     7201 gcttccagga atgccacatg caaattcaaa ccctgttaaa ctatggaatt tggcaatctt
     7261 cgttgtttca atcagattgg caccagcttc ggcttctaag gtataaccat ttaagtgcac
     7321 tgcatttagc ttatcaaaca tgatgacaaa gccacgaata ccaccatctc gcacaatgag
     7381 attgctggca tttcccaaaa caagccaagg catattttct ttgttggcat aaacaacgat
     7441 gcgtgatagc tcgtagtgat ttctaggaaa agccaagaaa tctgctgggc cacctacctt
     7501 agtgtaagtg taatgtttta ggggttcatt ctctcgaata tcaatcccat gaagttcagt
     7561 tatcatagtt atcttctttc ttttatcact tcttatacgc gtaaaaaaat cggcataaag
     7621 ccgatactat tatatcaaat tttagaaaaa ataacgatga atttggcaaa actttatgga
     7681 ttggcttata gcaggcatta ctccgaaaac agagtctaga actctgtctg ttgagctgaa
     7741 aagagcgtga tatctgattg atctaaagct gcaagataat cccttaaaag cttatgatta
     7801 ggaggcaatt ttaaatctgg ctgcaattcc aataagggga ttaaaacaaa agcacgctca
     7861 gtcatataag ggtgtggcac tttcaattcc tttgtgtcat aaacctcttc tccaaatagc
     7921 aagatatcaa tgtcaatagt tcttgatccc catttttcat gcctcacacg accaagagat
     7981 tgctcaatag cttgtgtttc ttttaagaaa tcagccgcgg ttaattgtgt atctaattga
     8041 caggccatat tgagaaaatc agcctggcct gtcttgcccc aagcagtcgt ttcataaatg
     8101 gatgattgag caagcaaccg tgtttgaggt aagtcagcta aagcctcaag agctttttgc
     8161 aagtaagctg ctcggtcccc catattggtg cctaaactta aataaacaat ggtcatctct
     8221 gtctctccaa ttcaatacca actgccttat agtggcctgc aatcggtggg ttttcctttt
     8281 taatggccac tttgatggct tcaataggtg gaaattcgtt aaaaagctgt tcacaaatcg
     8341 cacctgctaa acgttcaatc aaaataaact ttcccccttc cactaactga cggacactat
     8401 caaacaccat cccgtaatgg accgtatctg ttaattggtc tgacaaagaa gctgcttgca
     8461 aatcgactga caattctaaa tcaaccagaa aaatttgccc aagggtctgt tcttctttaa
     8521 aggctccatg gtagccataa aaacggcaac cttctaatac gattttatcc ataagtcctc
     8581 acatcaactg gctcaacaca gccacaatgt cttgattagc cttgacatcg tgtacgcgta
     8641 caatctgaca gccttttcca agggcataag cagacaaggc tgctgtcgct ccgtctcgct
     8701 ctttagcttt ggtgttgccg cctaacaagg catctacgac acgctttctc gaaataccaa
     8761 acaagacagg atagcccaac tgacagacgc ggtccaatcc ttttaataac tccgtattct
     8821 gttgaacaga tttggcaaat ccaaatcctg gatcaatcca aatattgttt tttggcacgc
     8881 cagcatcaag agctgcttgt gctctattgc ctagaaaatc acaaacgtct tgtgttacct
     8941 cttgataaac ttcttcgtct tggttatgca tcaagatgat aggcgcatcg tactcggctg
     9001 ctaaggcaaa catttgaccg tcgtacaaac ctgcccaaac atcattgaga atatcggcac
     9061 ccgcctctaa agctgctcta gctgtttcgg ttttataggt atcaatgctg attagaatat
     9121 catagttttc tttgatggcc ttgatcacag gaaccacccg gtcaatttca tcggtagcgc
     9181 ttacaaattg gcaacctgga cgtgctgatt ctccaccaac gtcgatgatt ttagccccat
     9241 cagcaatcat ttgctcaaca tgatctaatg ctttttgcac agtagtgtaa gaccccccat
     9301 ctgaaaaaga atctggagtc acatttaaaa tccccatgat agccgcattg ccctcaatca
     9361 caaactttcc aattttcata agttctccta atcttttgtc attagagaaa taacttcttg
     9421 tctctcagca cgactttcct tgtataagcc tctggcagtg gtggtaatgg ttttactacc
     9481 tggctttttg atgcctcgca tggtcatgca catgtgctct gcttctacca taactaaagt
     9541 ccctttagga ttgagggctt caactaaagc atccgctatt tgagaggtca aacgctcttg
     9601 gagttgaggt tgtttactag ctacttcaac tgctcgcgct aatttactca aacctgttac
     9661 tcgaccatca cttggtaaat aagcgatatg agccttgcca taaaaaggaa ctaagtggtg
     9721 ttcacatata gaataaaaac taatgtcttt gacaatcacc acatcctcat ggtgttcttt
     9781 aaagacagcc gtaaattcct ctttaggatc ttttcccagt cccaaaaaca tctccgcata
     9841 cattttagca acccgtttag gcgtgtcaag aagaccttct cgatttggat tttcaccaat
     9901 agcctctaaa aactgataaa tggctgcttc tgctttttcc ttattaatag acattagtct
     9961 ttcccttttc attagttttt tccttttcct tttcattttc tcttttttaa tgggttaaaa
    10021 gaacgctttt ttcaatatgt tttttccaat accgtctaat ttctgaaata aaatatagtg
    10081 accctgtaat cacgaaaaag tcatctgctt tagcatgctt acgcaaggcc aagaaatctt
    10141 tgaaatcagc aaccctacca aaacgttctg ggtatttttc caatggatag gcgttagggt
    10201 aatgaaagct agtaacctgc aagtcaccta tttgctcaag agcaaccaac atatctacaa
    10261 taggcttggt gtcgatcgca ccaaacaaaa tatgaagctt tttatcattg tagttatttt
    10321 ttaagacggc taccaaagca gcaacgcttt cattgttatg agctccatca atcattagat
    10381 ttggtgctaa taattcggta cggcctagcc acaaactatt tgccagacct tcccttatat
    10441 gatctggagt tagcctagga tagcggtctt gcaaggttaa gcaagtcata atagctaaag
    10501 ctgcattaga cacttggtgg tgtcctggca tagcgatgtg aatatctgaa ataactccta
    10561 aaggactagt aaaacgatag ccagatgcgt tctcagccat ttgaaactgc tcttgccatt
    10621 cccagattga agcaccaacc tgctcagctt ttgttaaaaa aacctctcga gctgacgggt
    10681 tttcaacggc aaaaacaagg gtctcgcccc cttctaaaac accagctttt tgggcggcaa
    10741 tatcagcgta agtctctccc aaaatagctt ggtgatctaa accaattgag ggacagacca
    10801 caaccattgc ttgaaaaaca ttggtcgaat cataaagacc gccaaggcct gcttcaataa
    10861 tagcaatatc tacaggatgc atatcaccaa aatagaggaa catgatcaaa gtaatcactt
    10921 caaattcagt tacctctcca aaatcagtct cttgaactag acgctctgtc aaaggacgaa
    10981 tacgattagc agcaataacc aaatcttttt ctgaaatcat tcgaccattt atgctaatac
    11041 gttctttgaa atccataata taaggagagg taaaggtccc cacttcataa ccagctgtcg
    11101 taaagatgtg ttgtaaatga ttgaccgtgg aaccttttcc gttagttcca acgatatgca
    11161 ctcccttgac atttttttgg ggatttccta actgtccaag aacccaaagc atacgtttta
    11221 agcctggctt gatgccgaaa actaagtgat catgaatcca ttctagagtc tcttcataag
    11281 tcatttcata cctcacatct atctttagta taccatatat aaaaaaaagt gacagcagat
    11341 aacctagagt agtacccttt tttttcttat cctcaccaaa cactcaaata agttctaaag
    11401 tacactacac ttttatcttc attgcttttt ttacgtgata agaataccag tcccacatag
    11461 tctttgctac ctcctaaagt gttatctgtg acaacaattt ttttactcct taatacacgt
    11521 tcttccaaaa aacaagaaaa aaggctgact tcaagccttc ttttctctaa aaaatgttac
    11581 aatagaggta cttatatcaa aaaaaggagc aagtaatgaa caatagacat aaacggcgcc
    11641 aaaaaagaaa agccttcact ctcattaacc ttatactatt atttgcctgc attcttggtt
    11701 tgaccctcat tttctattta tatcaagggt ctaacaaatt cggcttgtca gatgtctctc
    11761 aatttgtacc taaaataagt ctttttcatc acaaaaagac ggcaaaaaaa gaaacgacaa
    11821 aacttaagaa gactcatttc gattcctcaa aatctcaaaa aaaggcccat tctaaattaa
    11881 cttggaccaa gcaagaaaca cctgtcaaga tcccaatttt aatgtatcat gctattcatg
    11941 taatgtcccc tgaagagacg gccaatgcta atttgattgt caacccagat ctctttgatc
    12001 aacaacttca aaaaatgaaa gacgagggct actatttttt gagtcctgaa gaggtctacc
    12061 gcgctctttc aaataatgaa ttgcctgcta aaaaagttgt atggctaaca tttgatgaca
    12121 gtatgattga tttctacaac gttgcttacc caatcttaaa aaaatatgat gctaaagcca
    12181 ccaataatgt tattacagga ctaaccgaaa taggaagcgc tgccaatctg actcttaagc
    12241 agatgaaaga aatgaaacaa ttcggtatgt ctttccaaga tcatacagtg aatcatcctg
    12301 atttagagca ggctagtcct gatgttcaaa caactgaaat gaaagattca aaggactatc
    12361 ttgataagca attaaaccaa aacactattg ccattgctta tccatctggt agatataatg
    12421 acactacctt acaaattgca gctagactta actataaatt aggtgttacc actaatgaag
    12481 gaatagctag cgcggctaat ggacttcttt ctcttaaccg gattcgtatc ctcccaaaca
    12541 tgagtcctga aaatctttta caaacaatgg aaccataaca tcaatttttt tatcattcca
    12601 aatcatactc actgtaaact aaaaagacag ttatgaaaaa attctttcat aactgtcttt
    12661 tttgattgtt ttaaaattat tttattctta tctttaacgt cttttttcaa gaaaatctcg
    12721 ccaccacagc tgtaagtcag aggctttttt aagagattgg ctagctttaa gaggaatacg
    12781 cttaggttcc tttaaatacc tcactgactg atctgttatt cttggagacg tcaaataagc
    12841 aaccacttca cctttagtaa tcggtgctag taaagaagtc tctgatatca taaattgttt
    12901 aggtttggga acttgttcac cctgttgcaa aatgatggtt acatcttcgg caacagatac
    12961 cggtagttct tttacttggc catcttgaat agggagcgat cgctccgaga caagacttcc
    13021 ttttttaaga atcttacgac gacaataagt tctatccaca tagtccaaca aacgatttgc
    13081 ttgctcaaat cgcgtgtttt ggtttttatc agatccatcg gcatttagaa taacggtaat
    13141 cactcgcata ccattttcaa tagaagttac cacaagacaa tgacctgcca agtctgttgt
    13201 cccagtcttt aaaccatcca ctcctgctct acccttagcc atacctggta agagttgatt
    13261 aaaactttta atagaattgc cagcaaaatt aacttctgtt tgtttggtga tctctaaaat
    13321 ctcaggaaaa tcttctaaca aatgcatcgt cacaactgca acatcttgag cactcaaggt
    13381 attttcttct tccaacgcag aaccaggata acgattgtcc tttaacacct catttggcag
    13441 acctgaggcg ttgaggattt taccagaagt aatcccccaa tgagatagct gttctctcat
    13501 ttgatttaca aaatgaagct ctgaatccgc cactttttcg gctaacgcaa tagcagcgct
    13561 atttgagctt gctaccaaag tagctgtcaa caggtcttga actgaataag tttttttgtc
    13621 aaaaggtaca ttacttaaag acctatcttt tgttaactcg aaggcataat ctgagagatt
    13681 aactcgatct gataattgca gctggcctga tttaacctta tctaaaacca gataagccgt
    13741 caccaatttg gtcaaagagg caatagcatc tggcgtctta gcgtctttct catacagaac
    13801 tctcccagag tctgcctcta cgacaatcgc atgttctgct cccacttgaa aatcagctgc
    13861 gaggactatt ttactaagcc ctgaacaagt taataccaca aaaaaaagga tagcccacct
    13921 ttttatcatt atatctttca tagttactat tataacaaaa attctcagaa atttggaagg
    13981 aagtatagtg gcacacacct ttagtagctt aaacaagctc ataaagcaag taccctgtaa
    14041 caataacaca tcctgttata tgggcgatat aataagcttc gttatatcaa ttagttgttt
    14101 ggcaggaatt ttggtataaa atggaatcta atcctgaact tattacattt tgggttattt
    14161 cttcagatac tgttttatct aatgcattaa accagtgact atatgtattc aaagttgtag
    14221 acttatcagc atgtcccata cactttacaa cgtacagaat gtcttttttc aaaacattaa
    14281 taagatagga agcatgacta tgtcgcaaga cgtttcccgt tatcactggt actcctactt
    14341 cttgagcttt gtgcttaata actcgacaaa tcgttgattt acaaaatggt tcaccaaacc
    14401 gtgaaataat gaaatctgtg tcagtattga ctacttgatt tcttctccaa ttttccaata
    14461 ctactattgt cacatcatct aactcaataa gacgttctcc tgctggcgtt tttgtttggt
    14521 ctttacgata ccaatttcca ttttcatcct tttccaaggt tgtatgaact ttcaaaaatt
    14581 tttgttcgaa atcgatatct tcccatcata atgataaacc ttcacttaca cgaacacctg
    14641 tcatataata tagccaaata gttgtaaatc tttgtagctc ttcataatct tgcaaatcaa
    14701 acgattttat aaaattctga aactcaatat aagtccaaaa tggagtatcg ggatgtttcc
    14761 ctttaggatt atccaaagcc ttacacggca cttttgaaat atagtctaac ctttcagcat
    14821 accccatgca agctttgaat cgcgaccata aattctttgc ataattttca ataatatgta
    14881 aactaaaaga ttcacaatct caagctgtta ttccctttaa aagtttagaa ccaaaatact
    14941 gaataaataa cttatgatgt ggtaaggcta tttcataagt tacagcctga accttttgct
    15001 tatatgctct taggtaaata gtattcatat agttttcaaa cgatatattg tcattttcaa
    15061 tactaagatt attactaaac tcatacctaa tccgaaccag ttcatcatac gcttctttca
    15121 ttgtaccaaa tggctttcca tactaattct ttctggcttt cttttgaact cgtttacctg
    15181 ttactggatc tacaccaaga tcagcttgat aaaatatctt accattctta tcacaataaa
    15241 cacctttgta ctttttttga ttttttatag tcatctttaa ttctcacttt ctaaactttc
    15301 ttcagagagt agaataccta ctaactcttc tacaatatct ttaggagcaa taccaagtct
    15361 tctattgtca aagggcgatt tacttaattg taccgcattt tggtcaattt cgcgagcttc
    15421 ttcaaacttt tttatagcta ttctttttgc ttctctaata atatttcttg cagtatgttc
    15481 gagaaaacca atattcatca agctttttat atgttactat tttcacacct ccactccttt
    15541 caaaaaaatt gcaacctccg tgggtatgac tggttaaatc actcatggga atctcaccca
    15601 ctggcctttt acgctggcag cctgaacgtt cagaagtatc attgtcccta tttgtggtca
    15661 tggcagatag agaaccactc tattttatag ctaataatta tcgctcctat cacggcgtat
    15721 ctcactaaat tgctcgacaa acaagaaagt cccacctcgg tttattcagt tgtcaatgaa
    15781 caaatcaagg cgagactttt atttattatg aaaacgatac ggataatctg tttgccctaa
    15841 taaataatca atactgacgt tatagagttt agaacatctg ccattaaggc aacctctcct
    15901 ctctcaattt ttgcataatt tgcatgagag aaggaaagta aatttgcaac aaacttttga
    15961 gtgaaatcgt tatcttctct caaattccta atacgcgggt acatctaaca ttctcctaat
    16021 atcagtataa aggagatttg attaaaaaat gtt
//
LOCUS       DATURV010000033        15607 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DATURV010000033 DATURV010000000
VERSION     DATURV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15607
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.033"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            70..723
                     /gene="grab"
                     /locus_tag="VCF75_001063"
     CDS             70..723
                     /gene="grab"
                     /locus_tag="VCF75_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G-related alpha2-macroglobulin-binding protein
                     GRAB"
                     /protein_id="HEP2546631.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALADQA
                     DALQSEEAAVVQSDTAASDALEALADQTDAVQSEEASVVKADTAASDAWEKAATPTAL
                     DVKKTKDTTPVVKKEERQNVNTLPTTGEESNPFFTAAALAIMVSTGALVVSSKCKEN"
     gene            961..1503
                     /locus_tag="VCF75_001064"
     CDS             961..1503
                     /locus_tag="VCF75_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2546632.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1512..2795
                     /locus_tag="VCF75_001065"
     CDS             1512..2795
                     /locus_tag="VCF75_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP2546633.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCENSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2811..3671
                     /locus_tag="VCF75_001066"
     CDS             2811..3671
                     /locus_tag="VCF75_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEP2546634.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3673..4638
                     /locus_tag="VCF75_001067"
     CDS             3673..4638
                     /locus_tag="VCF75_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEP2546635.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4749..6032
                     /gene="aroA"
                     /locus_tag="VCF75_001068"
     CDS             4749..6032
                     /gene="aroA"
                     /locus_tag="VCF75_001068"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEP2546636.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            6025..6516
                     /locus_tag="VCF75_001069"
     CDS             6025..6516
                     /locus_tag="VCF75_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP2546637.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSVIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6724..8175
                     /locus_tag="VCF75_001070"
     CDS             6724..8175
                     /locus_tag="VCF75_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP2546638.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDTPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8224..9615
                     /gene="rlmD"
                     /locus_tag="VCF75_001071"
     CDS             8224..9615
                     /gene="rlmD"
                     /locus_tag="VCF75_001071"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP2546639.1"
                     /translation="MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVD
                     NALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQ
                     LTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
                     SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVHRGHYSGE
                     MMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTI
                     TDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFA
                     KTVKAVYGVEVIEAAVRDAQHNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVD
                     PPRKGLTESFIQASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQT
                     HHVECVSLLEKRS"
     gene            9767..10548
                     /locus_tag="VCF75_001072"
                     /pseudo
     CDS             9767..10548
                     /locus_tag="VCF75_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            10633..11034
                     /locus_tag="VCF75_001073"
     CDS             10633..11034
                     /locus_tag="VCF75_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEP2546640.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(11139..12623)
                     /locus_tag="VCF75_001074"
     CDS             complement(11139..12623)
                     /locus_tag="VCF75_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEP2546641.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIKASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            12997..14217
                     /locus_tag="VCF75_001075"
     CDS             12997..14217
                     /locus_tag="VCF75_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2546642.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLGL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            complement(14337..14702)
                     /locus_tag="VCF75_001076"
     CDS             complement(14337..14702)
                     /locus_tag="VCF75_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEP2546643.1"
                     /translation="MTIYSHTYETRPNHSAKAMSSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            14817..15548
                     /locus_tag="VCF75_001077"
     CDS             14817..15548
                     /locus_tag="VCF75_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP2546644.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKTVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVVGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
BASE COUNT         4850 a         2734 c         3307 g         4716 t
ORIGIN      
        1 ttaattataa tttccaaatt atcggtaatt taatatgcta atgcatataa aaataaaaaa
       61 ggagaaacaa tgggaaaaga aataaaagtg aaatactttt tgcgtagatc agcttttgga
      121 ttagttgcgg tgtcagcatc agtattagtc ggttcaacag tatctgctgt tgactcacct
      181 atcgaacagc ctcgaattat tccaaatggc ggaaccttaa ctaatcttct tggcaatgct
      241 ccagaaaaac tggcattacg taatgaagaa agagccattg atgaattaaa aaaacaagct
      301 attgaggata aagaagctac gacagctata gaagcagcaa gttcagatgc cttagaagca
      361 ttagcggatc aagcagacgc tttacaatca gaagaagctg cagtagttca atcagatacc
      421 gctgctagtg atgccttaga agcattggcg gatcaaacag acgctgtaca atcagaagaa
      481 gcttcagttg ttaaagcaga taccgctgct agtgacgcct gggaaaaagc agcaactcca
      541 accgctttag atgttaagaa aactaaagat acaacacctg tagttaaaaa agaagaaaga
      601 caaaacgtta atacccttcc tacaactggt gaagagtcta acccattctt tacagctgct
      661 gcgcttgcaa taatggtaag tacaggtgcg ttagttgtaa gttcaaagtg caaagaaaat
      721 tagttgctat ttgttctagc aaatgaaaca agagaatcga aagattctct ttttctgata
      781 tttaaaaatg ctacagttta taataccttg aaagactaaa tgttctcaaa ctcattagaa
      841 caagctttaa taaaactaac ccagttgtat caaaggatag ataaagacta agactgttat
      901 agaatatttg caaaagattt gttataatgg acccaataca ggtattgtga aaggtataat
      961 atggatattt ggacaaagct tgcagtgttt gctttttttg agactccgaa agtaatatta
     1021 cggccttttc gctatgaaga tcattgggat ttttacagca tggttaacga cactaaaaac
     1081 ctttattatg tttttccaga acaaaaaact aaggcagcaa gtgactatct tttagtacat
     1141 agttttataa agtttccttt aggtcagtgg gcaatagaag ataaagcaac ccaccaagta
     1201 ataggttcta ttagaattga gcattatgat gctaaaacgc gttgtgctga tattggctat
     1261 tttttaaact atgccttttg gggacaagga attatgacag aagtcgtaat aaaacttgtt
     1321 tatttatctt ttcacgaatt tggtctcaaa acgttgcgta ttataactca tttagaaaat
     1381 aaggccagcc agaaagtagc taaaaaagca ggttttcaac taaaaacctg ttttaaagga
     1441 agtgatcgta atactcataa aatctgcatt tataaaatgt accaactaac taatgatagg
     1501 tgataatcat tatgagcaaa catcaagata ttttagatta tttagaaaaa cttgctattg
     1561 gtaaaaaagt gagtgttaga agcatttcaa accacttaaa agtaagcgat ggaacagcct
     1621 accgtgctat taaagaggca gaaaatcgag gtattgtcga aaccaaacca agaagtggaa
     1681 ctgttcgtat tgaaaaaaaa ggacgggttc gtattgaccg tctgacctat tccgaaattg
     1741 ctcggatcag tgattcggaa gtgctagcgg gacatgctgg tttaggacat gaattcagta
     1801 ggttttcaat tggtgctatg actcaacaaa atattcgtcg ttacttggtc aaaggagggt
     1861 tattaatagt tggggatcgt gaaacgattc aactcttagc tcttgaaaac cataatgcca
     1921 ttttggtgac gggcggattt ccagtatcca aacgtgttat tgaaatggca aataaccaac
     1981 gaatcccagt tatggtaact cattatgata cctttactgt ggcaacaatg attaatcacg
     2041 cgctatctaa tattcgtatt aaaacagatc tcaaaacagt tgaacaggta atgataccta
     2101 ttacagatta tggttacctt tgcgaaaaca gctctgttga agaattcaat acattaataa
     2161 aaaaaacacg acaagtcagg tttcctgtat tagattataa aagaaaagtg attggtgtag
     2221 ttagtatgcg tgatgtggtg gatcagttgc caacaactaa gcttaccaag gtcatgtcta
     2281 aaaatccaat aacggctaga ccaaatacta gtcttgctaa tattagccag aaaatgatct
     2341 ttgaagattt aaacatgtta cctgtcactg acgaagaaaa caatttactt ggtatgatta
     2401 ctcgtagaca agcaatggaa aatttgccaa atcaccaacc caataacccc tatacttata
     2461 gtgagcaaat actttctaac cttgaagaaa cggttgatta ttaccaagta gtagttgagc
     2521 caacaatgat tgatagtgct ggtaacatgt caaacggtgt tatttcagag ttcttaaagg
     2581 aaattagtat cagagccctc acgaaaaagc accaaaaaaa tatcattatc gaacaaatga
     2641 tggtatactt tttacatgct attcaaattg aggatgaatt aaaaatatat cctaaaatca
     2701 ttactgaaaa tagacgaagc agcaccattg atattgaaat ttttgtggat gatcaagtga
     2761 ttgctaaagc tattattaca acgaaaataa attagaggag taacaaaaca atgataacat
     2821 taaaatcagc ccgtgaaata gaagcgatgg atagagcagg agacttctta gcaggtatcc
     2881 atattggctt aagggatatt attaagcctg gtgtggatat gtgggaagtg gaagcatatg
     2941 ttcgtcgccg ttgtaaagaa gacaacgttc ttcctttgca aataggggtt gatggtcaca
     3001 tgatggacta tccttatgcg acctgttgtg gtctaaatga cgaagtagca catgcctttc
     3061 cccgtcatta cattctgaaa gaaggggatt tgcttaaagt tgacatggta ctcagtgagc
     3121 ctttggataa atcaattgtt gatgtagcag ctttagattt tgataatgtg cctgagatga
     3181 agaaatggac aggatcttat actggtggtt tagctgactc ttgttgggct tatgctgttg
     3241 gaacaccgtc tgacgaaata aagcaattaa tggatgtcac caaagaagcc atgtaccgtg
     3301 gtattgaaaa agcagtaatt ggcaatcgta ttggtgatat tggcgcagct gttcaagaat
     3361 acgctgaaag cttcggttat ggtgtcgtgc gtgatcttgt tggtcacggt gttggtccta
     3421 caatgcatga agagccaatg gtaccaaact atggaacagc aggtcgtgga cttcgtctaa
     3481 aagaaggcat ggtcttgact gttgagccaa tgattaatac aggaacttgg gaaattgata
     3541 ccgacatcaa gacaggttgg gctcataaaa ccctagatgg tggtttatct tgccaatacg
     3601 aacaccaatt tgtgattact aaagatggtc ccgttatttt aacaagtcaa ggagaagaaa
     3661 gaacttacta aaatggcaga aaaaaaatgg tttgataagg tactgtccaa gtggcagtat
     3721 gaacccattc aagtctttat gaggcattta caaagtgcag aaatggattt atctgccatt
     3781 gcggttgcgt attaccttat cttaacggcc tttccattaa ttgttattgc agccaatatt
     3841 tttccttacc tcaatattga tattgctgat ttgttacgtt taatgaagca aaatttgcct
     3901 aaagatattt ttagaccggc ttctgcaatt gtagaaaata ttttctctaa accgtctgga
     3961 agtgttttgg gagtagcgac tttgacagga ttatggacaa tgtctcgaag tttgacatcg
     4021 ctgcaaaaag ctattaataa agcctatggc gcttctcagc atcgagattt ctttatcgga
     4081 cacttggttg gtttgttgac cagtttaatt attttatttt tattagcctt tgccttaatc
     4141 ttttcaacat tttctaaagc tgctattcaa gtgctggata agcattacca tttgagtgat
     4201 aatatcacaa cgattttctt actcctcatt caacctatta cagttttgat tatttttgtt
     4261 ggcttgatgc tactatattt tttattaccc aatgttaaga ttaaaaaaat tcgctacatt
     4321 ttaccaggaa ccctatttac atcttttgtg atgacatttt tgagtaatct ggttggcaat
     4381 tacgttgtgt ataatgttga gcggatggta gatattaaaa tgtttggttc agttatgatt
     4441 tttatcatca tgctatggtt tattttctta gcaagaatcc tgattttagg agctatattt
     4501 aatgcgacct atcaagaaat gtccttgggg aaattagaag gtcgtagtgg tgatatgatc
     4561 gctatcctta aaaaaacact tgggaatgat tcagatctat ctccttcaca gtctatcgaa
     4621 gatagtcaca ctgattgata caaaaaagat aataagtcgt ggagtgagaa actattttct
     4681 tgcttcattt tttatgagga taaatagaaa atcattgatc ttagcaaata gaaaggcaga
     4741 taaaacggat gaaattgcga acaaatgcag gaccattaca gggaacgatt caagttccag
     4801 gagacaagtc cattagtcac cgggcagtca tattaggagc tgttgctaaa ggggaaacac
     4861 gagtaaaagg actcttaaaa ggagaagacg tgctttccac gattcaagcc tttaggaatt
     4921 tgggggtccg tatcgaagaa aaagatgacc agcttgtgat agaaggtcaa ggttttcaag
     4981 gattgaccgc tccttgtcag acacttaata tgggaaattc tggaacctct atgcgcctca
     5041 ttgctggctt attggcaggt caacctttta gtgtcaaaat gattggcgat gaaagcttat
     5101 ccaaacgtcc tatggatcgg attgtttatc cgttaaagca aatgggggtt gagatatctg
     5161 gagaaacaga taggcaattt cctccactac aattgcaagg taatcgtaat ttacaaccaa
     5221 tcacatatac tttgcctatc tcttctgctc aggttaagtc agctattttg ttagcagctt
     5281 tgcaagcaaa aggaacaaca caggttgttg aaaaagagat tactcgaaac catacagaag
     5341 aaatgattca acaatttggt ggacgattga tagttgatgg taaacgcatt actttggtgg
     5401 gaccccagca gctcactgct caagagataa ctgtccccgg agatatttct agtgcagctt
     5461 tttggcttgt cgccggctta attattcctg gttcggagct ccttttaaag aatgttggag
     5521 tcaatccgac acgaacaggg attcttgaag tggttgaaaa aatgggagca cagattgtct
     5581 atgaagatat gaataagaaa gagcaagtaa ctagtattcg agtggtatat tctcacttga
     5641 aaggaactat catatcagga ggtcttatcc ctcgattgat tgatgaactt cccatcattg
     5701 cacttcttgc aacccaagca caaggaacaa cctgtattaa agatgctcaa gaattacgag
     5761 tcaaagaaac agatcgtatt caagtagtaa ctgatattct taacagtatg ggagctaata
     5821 tcaaagcgac agcagatggc atgattatca aaggccctac ggtactatat ggggctaaca
     5881 caagtaccta tggagatcat cgtattggta tgatgacagc aattgcagcc ttgcttgtta
     5941 aacaaggaca ggtccatttg gataaagaag aagccatcat gacgtcttat ccaacgtttt
     6001 tcaaagattt agaaaggctg tgtcatgact aaagtgttat tgggatttat gggagtgggg
     6061 aaaacaacgg tttcgaaaca cctaagtatg cactgtaaag atatggatgc tattattgaa
     6121 gctaaaatcg gtatgtctat tgcagctttt tttgaacagc atggagaaat agcatttcga
     6181 acaattgaaa gtcaagtgtt aaaagatcta ttgtttgcaa atgataatag tgtcatcgta
     6241 acaggtggcg gtgtagtggt tttacaagaa aatcgacaat tactgagaaa aaatcaccag
     6301 cataatattt tattggtagc ttcttttgaa actttgtacc agcgtttaaa acatgataaa
     6361 aagtctcaaa gacctctatt tcttaagtac tctaaggaag cattttatga attttatcaa
     6421 caacgtatgg tattttatga gggactttca gatttagtaa tcagagttga ccatcgtacg
     6481 cctgaagaag ttgccaacat cattgagggt tattagtagg tcattgtcgt aatctgatat
     6541 ttatggtttt aaacaggtat ctgtctaaag cacttcttat ggcaagttgg ctttattaaa
     6601 tattaggaaa taatcttgat tattctataa tgattaaaat tttgagagga caatctggct
     6661 aatataggtt ataatggtag tattcgttaa aagtgcaatt gtaatactaa gagatgataa
     6721 gatatgacaa aataccccat gggtggcctt agtcaccatg aagaattacg ttatttttat
     6781 ctcctaagga atttgagtta ccttagtgag aatgaaaaaa aagagtttgc ttttttaaaa
     6841 tctaaattgg aaattggtcg agcttatgct ccttctaaac agcattatag gaagtcaaaa
     6901 cggcaagaac cttactttga agatgactac tataacgact actcaccaaa tgatttacta
     6961 gaagatgatg acgtcaatca tgactcttct tttgttccat atggtttacc gatatatcct
     7021 aaagaagaca gatatttaaa caaaaaaaca aaacttacag ctcgtagacc tatagatact
     7081 ccgcagccta ttgatgaaga cgatgctttt ttaacagagt ctgttgctcg ttgtgctctt
     7141 cctagatctc aaaaaagaaa gcataagaaa aaaggatgta tgaaatggtt ttttaacatt
     7201 cttggcttat tgctaatgac tgtgcttatg gggttagggt tgatgtttgc aaaaggggtt
     7261 tttgatattt ctactaataa agctaattac aaaccagctg tttcccaagc ttttgatggt
     7321 caagaaactc aagatggtac gaacattctg gttttgggtt ctgaccaacg agttactcaa
     7381 ggttctacag atgctcgcac agataccatt atggttgtca atgtcggcaa ccatgctaaa
     7441 aaaataaaaa tggtctcttt tatgcgtgac actttgatta acattccagg atacagttat
     7501 aatgataatt cgtatgatct taaattaaat agtgcattta atcttggaga gcaagaagat
     7561 caccacggag cagagtacgt tcgacgggcc ttaaaacata attttgatat cgacattaag
     7621 tattatgtga tggtagattt tgaaacattt gcagaagcta ttgacacctt gtttccaaat
     7681 ggtgttaaga ttgatgccaa atttgcgaca gtaggtggtg tagctgttga ctccgtagaa
     7741 gttccagatg acctgcgaat gaaaaatggg gttgttccca accaaaccat cgaagttgga
     7801 gaacaacgca tggatggtag aaccttgctc aactatgctc gtttccgtaa agatgacgaa
     7861 ggagatttcg gacgaaccgt ccgtcagcaa caggttatgt cagctgttat gtcccaaatc
     7921 aaagacccaa ctaaactctt tacagggtct gcggctattg gtaaaatcta tgcgctgact
     7981 tcgaccaatg tttcttttcc atttgttgtt aaaaatggtg tttctgtttt aggaagtggg
     8041 aaaaatggag ttgagcacgt gaccatccca gaaaacgggg attgggttga cgaatacgat
     8101 atgtatggag gacaagccct ttacatagat tttgataagt atcagaaaac gttagccaaa
     8161 ttaggtctgc gctaagatat ggtataatgg agagtaacta aagttgctct tcttttttga
     8221 gcaatggtta gccctagaaa aggaaaaaga ataagaatgt tgaaaaaaaa tgatattatc
     8281 caagttgcta tttcagattt aagccatgag ggagcaggtg tcgctaaaca cgatggtttt
     8341 gttttctttg ttgataatgc cttgccagaa gaagtgattg acatgcgtgt cttaaaggtc
     8401 aataaaaaca gcggttttgg caaagtagaa gcctatcatt acctgtcgcc agctagaaat
     8461 gcagatgtca atttaactta tttgcgaaca ggcattgcag accttggaca tctaacttat
     8521 gaagaccaac tgactttcaa aaagaaacaa gttcaggaca gcctctataa aattgctgga
     8581 atcagtgacg tgacagtcga aagcacgatt gggatgacag aacctttagc ttaccgaaat
     8641 aaggctcaag ttcctgttag acgtgttaat ggtcagctag aaacaggctt tttccgcaag
     8701 cattctcatg acttgattcc tatttcggat tattatattc aggacaaaga gattgatcgt
     8761 ctcattaatt ttacccgtga tttattacgc cgctttgata ttaaaccgta tgacgaaaca
     8821 gaacaaacag gtctcttgag aaatattgtt gttcaccgag gccattactc tggagaaatg
     8881 atgctggtct tagtgactac acgcccaaaa gttttccgtg ttgaccaagt cattgaaaaa
     8941 atcgttgaag cttttccagc agtggtgtct attattcaaa atattaatga taagaacacc
     9001 aatgcaatct ttggtaaaga tttcaaaacg ctttacggca aggataccat tactgatagc
     9061 atgcttggaa acaactatgc catttctgcc cagtctttct accaagtgaa tacggttatg
     9121 gcagaaaaac tctatcaaac agctatagcc ttttcagacc tttctaaaga tgacattgtg
     9181 attgacgcct actcaggtat cggaaccatt ggcctatcat ttgctaaaac agttaaagct
     9241 gtttatggtg ttgaagtgat tgaagcagct gttagagatg ctcaacacaa tgccgctctt
     9301 aatggcatca ccaatgccta ctttgtcgca gataccgcag aacatgccat ggcaacatgg
     9361 gcgaaagatg gcattaaacc aagtgttatt ctggtagacc cgccacgaaa aggcttaaca
     9421 gaaagtttca tccaagccag cgtggcaatg ggaccacaaa aaattaccta cgtttcctgc
     9481 aacccagcca ccatggcgcg tgacatcaag cgttatcaag agttgggtta caaactggcc
     9541 aaagtacagc ccgtagattt gtttccccag acgcatcacg tggagtgtgt aagtttactt
     9601 gagaaacgga gttaaagagt ccgcctcact ctgttatttc aaggtgagac gctcaaaagg
     9661 tccactggac cttttgactc ccgcagaccc atcacgtcga gtgcgtagtg ttgctaatca
     9721 aggagtaagg cagctgcctc cacatgacaa aaataaggag aaacgaatgc ctattaccaa
     9781 acacgagatt ccccttttag aatgtgatag taatccatca gccgtcatta tacctacaca
     9841 tgaaggtcta cagctaagtg tgtctatgct ttcttagaag acgagattga agcttttgct
     9901 ttagaccatc aaggtaaaaa agtagcagaa ttcatttcag ccacaaaggt ttaccctgtt
     9961 tatcagatac cttataaagg ccaagacatt tgcctagctc aagctcccgt tggctcagct
    10021 cctgcggcgc aattcatgga ctggctgatt ggttatgggg ttaaagaaat gatcagtaca
    10081 ggaacctgtg gcgttttggt tcctatcgca gaaaaccgtt ttttagtgcc agttaaggct
    10141 ttgcgagatg aaggaacaag ttatcattac gtggcaccat cttgttatat tgatattgat
    10201 cctaagatgc ttagaccgat agagaaaacg ctttcagcac aggggttagc ttatcaagag
    10261 gtcatcacct ggtcaacaga tggtttttac cgagaaacca aagaaaaagt ggcttatcgc
    10321 caggaagaag ggtgttcggt tgttgagatg gaatgttctg ccttagcagc agtagctcaa
    10381 ttaagaggca tcctttgggg acaactctta tttacagcag atactctagc agatgtagag
    10441 gtttacgatc aacgcaaccg gggcgctgat tcctttagtt ttgcgcttca tttatgcttg
    10501 gaagtgttca atacgctaga aaaggatgga aaggctactg atttttgaaa catgagctct
    10561 gccttctgtt cctatttgta gtataatgag gcttgtattg cttttagact aagcttagaa
    10621 aagagaaaaa caatgtcaga tttaacacaa gagatgacct taaatgttat tagtattttt
    10681 gataattacc aaattgaact tgctgaaaaa ggacacctta ttttaagtac tgaagtgact
    10741 gagacggctt tgaattacta tgggaatgct catggtggtt atcttttcac tctatgtgat
    10801 caagtaggtg gtttggttgc tagaactact ggcgtagaat ctgtcacctt acaggccaat
    10861 gctaactatt taaaagcagg gcacaaggga gacaagctga tggttgaagg tcgattggtt
    10921 catggaggtc gaaccacgca ggtggttgat gtgacgattc ataaccaaac aggagctctt
    10981 ctaacaaaaa caagcttgac catgtttgtg actggccgac gtcataacgg gtgattaatg
    11041 aatcaataaa ggaaaaaagt cccaactctc tgagaattgg gacttttttc ctttattgag
    11101 agataccata aatcacccgt tagacgggtg gttatagttt atttacggta aagtttgtga
    11161 tgttggtaaa cagggtcaaa agtaatattt accccaagtt ttcggagtac atttttatct
    11221 tcttcggtta agataacggt agaatgggct tcgctacctt tcaaattgcc gagttctttc
    11281 atggctagat tagcttcttc actagtcata gcagtaatgg ctaacgcaat cagaatttca
    11341 ttggaatgaa gccgcggatt gtgactgcct aagtggttca ctttgagccc ttgaataggt
    11401 ttcacgtatt cgggttcaat caaatgagtg gttttatcaa tctttgccaa ggttttaatc
    11461 gcattgatga taacagctgc agttggtcca aagagttcag atgttttacc tgtcacaatt
    11521 tgcccatttg gtagttggag agcaagggca ggttggcctg tttgctctgc tttttgatga
    11581 gcagctacag taacgtgacg atcgtcaggg gtgacaccga tatcattcat caagagctca
    11641 atttttttga cagcagattc tgtgacccgc tctgccttaa aatccacgag ggtttggtaa
    11701 taacgacgga tgatttcttg tttagaagct ttgatagcag cttcttcatt gacgatagaa
    11761 aaaccaacca tgttaacacc catatcggtt ggtgaggcat aaggagactt gctcaaaata
    11821 cgctcgaagg tccgatttaa gacaggaaag acctcgatat cacggttgta attaacggcc
    11881 gttttgccat aagtttccaa atggaagggg tcaatcatat tcacatcatc taggtctgcc
    11941 gtggctgcct cataagccag attgacaggg tgatgaagag gaagattcca aactgggaag
    12001 gtttcaaatt tagcatagcc agacttaacc ccattgattt ggtcgtgata catttgggac
    12061 atgcaagtgg ctaattttcc agacccaggg cctggtgctg taacaacgat aaggttacga
    12121 ctcgttttga tgtaatcatt tttccccatc ccctcagatg agataatatg attaatatct
    12181 gtcgggtagc ccttgatagg ataatgcagg taagaagcaa tgccattttt ttcgagttgc
    12241 ttgcgaaagg catctgcagc gggttggtta ttgtattgag taataacgac agaacctacg
    12301 taaatatcta acgtgttaaa tttgtcaatt aagcgaaaga cttcttggtc ataagaaatg
    12361 cctaaatcac cacgcgcttt agaatgttca atattattag cgttaatggc aattacaatt
    12421 tcgacttgtt ctttgagctc tttgagtaat ttaatcttat tgtcaggttc atagccagga
    12481 agtacacggg cagcatggta atcttctaac attttaccac caaattccat gtaaagtttg
    12541 ccatcaaatt ggctgatacg ttctaaaata tgatctcttt gcaagtttaa atacttgtta
    12601 gaatcaaaag caatagtctt cattttttca cctctgtaaa gcattataac aaagaaatac
    12661 caagaaagaa aaggtttttt gcagggatac ctcatttagt gactttttaa aaaaacttag
    12721 gcttggttct tagaagaaaa atgtatccgc tatgttcaaa agaacttgac aattaaaatg
    12781 agctttacta tactagtaac aattaaatac tactttaaac atgttccgtg aagcatgaaa
    12841 gtcttaacag taaagagagt ttttttgtca tctcttatta tttagtgaac tttgatggac
    12901 ttgcggactt gtttccaagt ctttttttga cctttgggca aagggtattc aattaattaa
    12961 ggtagtcagt gagctactat taagaaggag acaattatgt caacattatc attggacacc
    13021 acaaataagc gagctttagt ggcagctatt gcagcttcag gaacggatga cttgaatgtc
    13081 atgtttcttg ccttttccat gtcttccatt atgacagact tgggtcttag tggcactcag
    13141 ggtggctgga ttgcgaccat taccaattta ggaatgttgg tgggaggcct cttattcggg
    13201 ttgctggctg ataggcacca caagtttaag gtctttaaat ggacgatttt actgttttca
    13261 gtagcaacag gattaattta ttttacccag tctttgccct acctgtacct gatgcgattt
    13321 attgctggca taggagttgg aggtgagtac ggtgtagcca ttgccataat ggcagggatt
    13381 gtaccaccag aaaaaatggg tcggatgtct tctttaaatg gtattgctgg ccagcttggc
    13441 tccattagtt cggctctttt agcagggtgg ctggctccaa gtctaggctg gcgtggcctt
    13501 tttctctttg gattacttcc gattctcttg gtgatttgga tgactttagc catagatgac
    13561 caaaaaattt gggatcatta tgggcaagag gaggaagaat gcagtcaacc aattaaaatc
    13621 aatgaactat tcaaaacaaa atccttaaca gctcaaaccc ttgctttaat ggtcatgacg
    13681 actgtgcaga ttgcgggcta ttttggaatg atgaattggt tgccaaccat tattcaaaca
    13741 agcttaaacc tttcagtaaa aagttcgtcc ttgtggatgg tggctactat tgtcggcatg
    13801 tgtttgggga tgctgtgttt tggtcagctt ttagattgct ttggtccacg tctgatttat
    13861 tccctctttt tattagcatc atctatttgt gtttaccttt ttcaatttgc caattccatg
    13921 gcgagtatgg ttattggagg tgctattgtc ggtttttttg tcaatgggat gtttgctggc
    13981 tacggggcta tgattaccag actttatccc catcacattc gatccacggc taataatgtt
    14041 attttgaatg ttggccgtgc tctaggagga ttttcgtccg ttgctattgg tagtatttta
    14101 gatgcctcag gtatttcaat ggtcatgatt ttcctagctt ctctgtatgt gattagtttt
    14161 ggagccatgt ggtctattgg tcagttaaaa gcagaacgct accaacaatt gcgttagcaa
    14221 agagatgggt gttagtattt gagttacttt cactcacaaa gctattttat tgattgagat
    14281 aaccaaaaag gtaagcactt agggattgct tacctttttg gttcttttct tttgttttac
    14341 tccgctactt tggccataaa atgtttactg ttcaccctaa ctctagtatg acaggctttt
    14401 ccaatgagtt gttctcccac aaaagcttcc aagcggaaat cgtattttcg accttcttct
    14461 ttaagggctg taatgatgat tgtaacagga tctccaatgg ctgaagctgc caaatgttgg
    14521 atagctatct cgctgccaac cgttgtaaag tcactgtcga gctgttcttg ggcaaataag
    14581 taagaagcat tttccataaa agcaacaaga gcaggagtag ctaggacatt cagcgttcct
    14641 gagctcatgg ctttagccga atgattggga cgggtttcat aagtgtgaga ataaatagtc
    14701 ataagttaat ctcctcgttt ctataagcct attataatat attaagcgct tacagtcatg
    14761 aaaccactgt ttttttatgt tatacttaat aatgaatttt ctttgaaagg gttatgatgc
    14821 gtttagataa atttttagtg gcaactggtg ttggaactag gagccaagtc aaacttctct
    14881 taaaaaagaa ggctatcttc gtcaatcaaa aggttgaaac ctctgccaaa gcacacattg
    14941 atgaatacaa ggatttggtg acgtatcaag gcacgccctt ggtctatgaa tcctttgttt
    15001 attacctgtt aaacaagccg tcagggtatg tgtcggcaac ccaggacaga cagcaggcta
    15061 ctgttatgga gttgttagat gacacggcta ggcaaaaaac agtttttcct gtgggtaggt
    15121 tagataagga tacccgtgga cttttgctct tgaccaataa tggacaacta gcccatgacc
    15181 ttttatcccc taaaaaacat gttacgaaag agtacttggc taaagtagta ggcatcatga
    15241 ctgaagctga taaagattat tttgctagag gtatttcctt aaaggatcac cagtgcctac
    15301 ctgctcattt agaagtgctt gctagtgatt tacagcagca aacaagtttg gttaaaatta
    15361 ccatacaaga aggaaaattc catcaggtga aacgcatggt agcagcctgt ggaaagggag
    15421 tgcttgactt gcagcgtctc agcatgggac cattaaaact agaccctagc cttgcagaag
    15481 gggagtttag gcgcctgacg cctgaggaat tgcaaagcct ggcaccttat tgtcagagct
    15541 cactataaaa agaaacctcc cctgtcagta ctgaccccaa aaagttggac aaataattat
    15601 tgaaagg
//
LOCUS       DATURV010000034        15599 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DATURV010000034 DATURV010000000
VERSION     DATURV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.034"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(92..2083)
                     /gene="uvrB"
                     /locus_tag="VCF75_001078"
     CDS             complement(92..2083)
                     /gene="uvrB"
                     /locus_tag="VCF75_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEP2546645.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            2276..4450
                     /locus_tag="VCF75_001079"
     CDS             2276..4450
                     /locus_tag="VCF75_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP2546646.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGNFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATISDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            4450..5190
                     /locus_tag="VCF75_001080"
     CDS             4450..5190
                     /locus_tag="VCF75_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2546647.1"
                     /translation="MTELKIDVQGLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEAAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(5338..5490)
                     /locus_tag="VCF75_001081"
     CDS             complement(5338..5490)
                     /locus_tag="VCF75_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546648.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(5487..6866)
                     /locus_tag="VCF75_001082"
     CDS             complement(5487..6866)
                     /locus_tag="VCF75_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP2546649.1"
                     /translation="MSHFSDKFMDISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLDTGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(7044..7493)
                     /locus_tag="VCF75_001083"
     CDS             complement(7044..7493)
                     /locus_tag="VCF75_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEP2546650.1"
                     /translation="MIISAEGDVSIHHCFEAMLISLQEDYRQNTLRRLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANHFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(7490..7825)
                     /locus_tag="VCF75_001084"
     CDS             complement(7490..7825)
                     /locus_tag="VCF75_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP2546651.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRCES"
     gene            complement(7828..8139)
                     /locus_tag="VCF75_001085"
     CDS             complement(7828..8139)
                     /locus_tag="VCF75_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2546652.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(8162..10156)
                     /locus_tag="VCF75_001086"
     CDS             complement(8162..10156)
                     /locus_tag="VCF75_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP2546653.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVSQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNIFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTTRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTEMFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVAICREPLEWDKVHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQTLLEEPNYSKFMTVFTPLIHD"
     gene            complement(10262..11356)
                     /locus_tag="VCF75_001087"
     CDS             complement(10262..11356)
                     /locus_tag="VCF75_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEP2546654.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAERSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEQIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGCIIIDNDRYHRYKGELQIALKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            complement(11365..12765)
                     /locus_tag="VCF75_001088"
     CDS             complement(11365..12765)
                     /locus_tag="VCF75_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP2546655.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSDAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGNPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            12990..13685
                     /locus_tag="VCF75_001089"
     CDS             12990..13685
                     /locus_tag="VCF75_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEP2546656.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            complement(13690..13848)
                     /locus_tag="VCF75_001090"
     CDS             complement(13690..13848)
                     /locus_tag="VCF75_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546657.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(13917..15230)
                     /gene="obgE"
                     /locus_tag="VCF75_001091"
     CDS             complement(13917..15230)
                     /gene="obgE"
                     /locus_tag="VCF75_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEP2546658.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDESDLVDEEAYYGFAEAEKEFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDLVRIGKFEFEFVD"
     gene            complement(15287..15415)
                     /locus_tag="VCF75_001092"
     CDS             complement(15287..15415)
                     /locus_tag="VCF75_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEP2546659.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIASALSVLM"
BASE COUNT         4700 a         3338 c         2794 g         4767 t
ORIGIN      
        1 aaaaagttaa cttaaaattg tcctaataaa aagaattatt tgactttaac ttatatatgt
       61 taataagtat aatataagct ttattcaaaa cctagtccat taatttaagt tctaaaatca
      121 agtctcgcat ttgagctgcc aattcgaaat caagtaattc agctgcctct tgcatttgtt
      181 tttgcagagc attgatcgct tcttttcgtt ctcctcttga catactttca taatccatct
      241 cttctttcga tatatcattg tggcttgttt tacttatact aataaggcct ctgatgtctt
      301 tttttatagt ttgtggaacg atgccgtgcg ctttgttata tgctatttga atctcacgac
      361 gacgagccgt ttcgtcaata gcccgctgca tagaatctgt catcttgtcc gcatacataa
      421 tgacatgacc atctacgttt cgagctgctc taccgatggt ctgaatcaag ccgcgttcat
      481 tacggaggaa accctcttta tcagcatcca aaatggctac aagactaact tctggcacat
      541 caatcccttc acggagcaga ttgattccaa tcaagacatc aaaaacacca agtcgtaaat
      601 cacgaataat ttctgtccgc tctaaggttt taatatcact gtgcatatac ttgaccttga
      661 cacccatctc ttttagataa tcagtcaaat cttctgccat ttttttagtt aaggttgtga
      721 tgaacgtccg ttcatcgcgc gctacgcgct ggttaatttc tcccaacaaa tcatccattt
      781 gccccatact tggccttaca tcaatttcag gatcaagtag cccagtcgga cgaataattt
      841 gctcaataat tgtattagtc tgtgacattt cgtattcacc aggcgttgct gaaacataga
      901 caatttggtg cacatgactt tcaaattcct cgcgtcttaa gggacgatta tcaagagctg
      961 atggcaatcg aaaaccatag tcaactagca tttgttttct ggcttggtca ccattataca
     1021 tgcctttgat ttgtcccatt gtcatgtgac tttcatcaat cataattaaa aaatcttctg
     1081 ggaagaaatc taacaaggta taaggaggtt ctcctggtga cctgccatcc atatgacggg
     1141 aatagttttc gactccgcta gtatagccca tttcacgtaa catttcaata tcatattccg
     1201 ttctttggcg taatcgctgt gcttctagta gtttaccttc agattcaaat aactgtaact
     1261 gctctgctaa ctctgcttga attttggcta ttgattgctc catatgctca tcatttgtta
     1321 caaagtgagt agctggaaat aaaaccaaat ggtcaacttc tccaatagtt ttcccagtta
     1381 aactttcgat ttcacatata cggtcaatct catccccgaa aaattccacc cgaaaagcat
     1441 gttcatccct ggatgctgga aagacctcaa caacatcgcc acgtacacga aaacagccgc
     1501 gttgaaaatc aatatcatta cgctcaaatt gaatatctac taattgattt agcaaagtgt
     1561 cacgtgatat ctcctgacct ggacgtaggc tgactgctga atctgcgtac tcttttggcg
     1621 aacctagtcc ataaatacaa gatacagaag ctaccactat gacatcgtta cgctcaagta
     1681 aagaagaggt ggctgaatgg cgaagtttat cgatttcatc attgactgaa ctatcttttt
     1741 cgatataggt gtcgcttgat gggacatatg cttcaggttg gtagtaatca taataagaaa
     1801 caaaatattc cacagcattg tctggaaaaa attctttaaa ttcaccatat aattgtcctg
     1861 ctaaggtttt attgtgagct atcactaagg taggtttatt tactttacta attacttggc
     1921 tcatggtgta tgtttttccc gttcctgtag cacctaggag aatctgagct ttttctccgc
     1981 cctcgatatt atcaactagg ctttcaatag cttgtggctg atcaccagac ggtttatact
     2041 ttgattttag tttaaatggt ttatcatcac gtttatcaat cattttatac cctctaatag
     2101 tcttactctc ttatagacta ttttaacata gataagccag aatcacctcc aataagcgta
     2161 aaaaacatgt catttacatg ttatgtttta tgacatataa ataacaaatt tcttgcttaa
     2221 tgttcaatat tttgttataa ttaaccatat tttttagttt tataaggaga ttagtatgac
     2281 gcacaaaata aaagtattgc tgcttgcgat aatgtctatt tttttgacat gcaatattgc
     2341 aagtgctgaa actattgcta ttgtttcaga tacagcttat gccccatttg aatttaaaga
     2401 ctcagatcaa atttacaaag gaattgacgt tgatattatt aatgaagtag ccaaacgtca
     2461 atcttgggat ttcagtatga gtttcccggg ttttgatgca gctgtaaatg ctgttcaatc
     2521 tggtcaagcg agtgctctaa tggccggtac aaccattacg aatgctcgta agaaagtctt
     2581 tcatttctca gagccatatt acgataccaa aattgtcatt gcgacacgta aagccaatgc
     2641 catcaaaaaa tacagtgact taaaaggaaa aacggtcggt gttaaaaatg gaacagcggc
     2701 tcaagccttt ttgaataact ataaaaaaaa gtatgattat actgttaaaa catttgacac
     2761 aggtgatctt atgtataata gtttatctgc tggttctatt gccgctgtta tggatgatga
     2821 ggcggttatc caatacgcaa tcagccaaaa ccaagatatt gctattaaca tgaaaggaga
     2881 gcccattgga aactttgggt ttgctgtcaa aaagggaagc ggatatgatt atctagttaa
     2941 tgatttcaat acagctctta aagctatgaa agctgatggt acctaccaag ctatcatgac
     3001 caagtggtta ggcacagatg ataaagctac caccagtcag gcaacgggaa atccatctgc
     3061 caaagctaca cctataaagg acagttataa aattgtctct gattcgtctt ttgcaccttt
     3121 tgaatttcaa aatggtaagg gcaaatacgt tggtattgac atagaattaa tcaaagctat
     3181 tgctaaacaa caaggtttca aaattgaaat cgctaatcca ggtttcgatg ctgccttaaa
     3241 tgctgtgcaa tctagccaag cagatggggt cattgctggt gcaactattt ctgacgctcg
     3301 taaagctatc tttgattttt ctgatcctta ttatacttct aatataattt tagctgttaa
     3361 agctggaaaa aacatcaaga actatgaaga cttagacaga aaaacagtcg gtgctaaaaa
     3421 cggcacttca tcttactctt ggttaaaaga aaacgctcct aaatatggtt ataatgtcaa
     3481 ggcatttgat gatggttcta gcatgtatga tagcttaaat tcaggttctg tagatgctat
     3541 catggatgat gaggcggttc ttaaatacgc tatctctcaa ggtcgtcgct ttgaaacacc
     3601 tcttgagggc atttctactg gtgaagttgg ttttgctgtc aagaaaggaa ccaatccaga
     3661 attaatcgaa atgttcaaca atggcttagc tgctctcaaa aaatctggtc agtatgatga
     3721 cattatagat aaataccttg actctaagaa agctgcaact ccttctgaaa aaggtgctga
     3781 tgagtctact atttcaggcc tattatcaaa taactacaaa caactattgg caggacttgg
     3841 aaccacgctc agtttaaccc ttatttcatt tgctattgct ataattatcg ggatcatctt
     3901 tgggatgatg gccgtgtcac caactaaatc acttcgactc atttcaacgg tctttgtgga
     3961 cgttgttcga gggattcctt tgatgattgt ggctgccttc attttctggg gagtaccaaa
     4021 ccttatcgag agtatgaccg gccaccagtc accgattaat gatttcttag ctgctacaat
     4081 tgcactgtca cttaatggcg gagcctatat tgctgaaatt gttcgcggtg gtatcgaagc
     4141 tgttccagca gggcaaatgg aagctagtcg aagtcttggt ttgtcttacg gaaccacgat
     4201 gagaaaagta attctcccac aagctgtgaa actaatgtta cctaacttta tcaatcagtt
     4261 tgttatttca ttgaaggata caacaatcgt ctcagcaatt ggtttagtgg aactcttcca
     4321 aacaggtaaa atcattattg ctagaaatta ccagtcgttc cgcatgtatg ctattttagc
     4381 aattatttac cttatcatga ttacactctt aacaagactt gcaaaacgtt tagaaaagag
     4441 gcttaactaa tgacagaatt aaaaattgat gttcaaggtt tacacaaatc ttatggtcaa
     4501 aatgaagtct taaaaggaat tgatgccaaa ttctacgaag gtgatgtcgt ttgtatcatt
     4561 ggtccttctg gttctgggaa atcaaccttt ctacgtaccc tcaaccttct agagacgatt
     4621 accagtggga aggtcatggt ggacggtttt gaattatcag atcctaagac taatattgac
     4681 aaggcacgtg aaaatatcgg gatggtattc caacacttca accttttccc tcacatgact
     4741 gttcttgaga atattatctt tgctcctgtt gaattaggta aagagtctaa agaagctgct
     4801 aaaaagcacg gcatggcact tctagaaaaa gtcggacttt ctgataaggc cgacgccttc
     4861 ccaggaagtt tatccggcgg gcaaaaacag cgtgtcgcca tcgccagaag tttagccatg
     4921 aacccagata ttatgctctt tgatgaacca acttctgctc ttgaccccga aatggttggg
     4981 gacgttttga acgttatgaa agacttggca gaacaaggca tgaccatgtt aattgttact
     5041 cacgagatgg gatttgctcg ccaagtagct aaccgtgtta tctttacaga cggtggtcaa
     5101 ttcttggagg atggtactcc tgaagagatt tttgatcatc ctaagcaccc tcgtctcatt
     5161 gaatttttag acaaggtctt aaacgtctaa tagagcagac accatactaa cttccaaaaa
     5221 ataataaggc aaggaaacct gtttccttgc cttttttggt tactgttatt attcaaacca
     5281 gcaaaatagg gatactctta tgactctctc cttcataaga gtgtccccta tgctttttta
     5341 tgggcgttta tgtttagtga ggtaataacc taatcccgta agagctagtc ccgtcactat
     5401 gtgtaagtat ttgttaccaa ccatcggact aatggcaatg agaaaaaagc caacagccat
     5461 taaaaaagtt gctccatttt tttctgtcat gcaaggtcac tcgctttttc tttattggcc
     5521 aaaatgacaa atggtgccca gataaggaag gcaacaaaga ggttaaacag tgaaaccaaa
     5581 cctgccataa tattacctcc agtatctagg taagcataaa gtccaacagg ggtaatccaa
     5641 ggtactgata agaataccgg tggaatcaaa cctattgcag tagcaaaata agctatgcta
     5701 gcacaaattg gtggcacaat tagccatgga atcacaaagg ttggattcaa aacaattggc
     5761 atcccaaatg taattggttc gttgatattg aaaacaccca ttggtgcgga caatttggca
     5821 atggttttat gctcttctcg ttttgagaat atgaagatag caataatcaa tgccaaggtt
     5881 actcctgaac cacccatttg agcataagca tcaaaggacc cgcgtgtcca aaggtatggt
     5941 aaattagcag cgctatgcgc tgtattgtag gcagctgtat tttctgttaa agctaccata
     6001 taaataccat ccatcactgg ggctaagaca ttatgaccat gcaagccgaa gaaccagagc
     6061 agttgaacta agaaggttaa caagataact gaaccaatac cctgtgataa gcctagcaat
     6121 ggtaattgga tataggttga aatgacatca cttagggaag aacctgttaa tgcgaagatg
     6181 aggtaggcga aaattgccga agcgtaaatc gcaactgttc ctggaataat ggcagcaaag
     6241 gctttattaa ccgcaggtgg tacattatct ggtaaggtaa tagtaatatt acgtttggtc
     6301 aaagccgcat acacaaaact tgataaaaag ccaataatta aagctgtgaa caagcctgtg
     6361 gcaccgacat actttagagc tattgctccc cattgactag tttcaatact tgaagctcca
     6421 cccgcagtaa cgactgaaag ccccatttct tttaaattgc taattaggtt agcagaggca
     6481 ttttcaagtg gtgtcgaaat ggttaaagtc tgaggtaggg tggcaataaa tgagccaaaa
     6541 gaaatcaatc ctcctgccaa tggattaatc ttatgcataa cagctaaatt gtaaccgaaa
     6601 gcaaaggcaa aaaagagcgc catgatacca attgtcccaa aataaacata gccattgatg
     6661 tcgataacag gttgcatagc ttttgcaaac cctgtccatc ccatattgtt tggaatatcg
     6721 cgtaaaaaaa cgttcaataa gacagcaact gaacctgcca tggtaatagg catcattgaa
     6781 ataaaggaat cacgaatagc tactaaatgc cgttgtgacc caatttttcc tgaaatatcc
     6841 ataaacttat cagaaaaatg cgacataaat cttctcctct ttttattttt ttaatgtaac
     6901 aaaggtaacc ttaaaagcag cggtgtctgc ctaacacaat tatctcaaaa tcatccttgt
     6961 taaacgtgat gcttagagat aaccggtaat cgactcttat ccaaatgaac ccttctctct
     7021 catccaaggt ctgtgaataa tcttcacaaa gacgatggtt gctgcaactg tttagcatat
     7081 tggataaggg ctgctaactg agcttgagat tggcgttcta ggcttttttt gaacagtttg
     7141 ccaacaataa aatgatttgc tttttgaaaa atatctagag gatgatagtc ataagaatag
     7201 gtaattctag tccgattctc ccccagagcc tctaatcgat aagccaattc ctttatccca
     7261 tgattagaac taaaacaagc agtatataat tgcggagctt ctagggccgt aataagcacc
     7321 ctcactgtac gctctttgta tttaccataa tgtttaacat agtttatccc cgctttgata
     7381 tcatctcttg acaaccgtct caaggtattt tgtcgataat cttcttggag cgatatcagc
     7441 atggcttcaa aacaatgatg aatggaaaca tctccttctg ctgaaatgat catgactcac
     7501 acctctgtat tgctgacatt ttgtcgtaaa cggtaaccaa ttccttggct aaatcaataa
     7561 aggctaaggc agtcatcatg tgatcttgcc catgcaccat caataaagac aattcaaccg
     7621 catcaccagc tgcttcttgg gacagcaaat gtgtctgcga tttatgggca atatttaacg
     7681 agtttgtcgc ttcttgaagt aagtgctgag cctcctcaaa ctgcccatct cgtgctgcat
     7741 gaatagcctc tacggccttg cccttggctt cgcctccata catgattaag cccataatag
     7801 cttctaagtt ggatggttcc gtcatgccta ctcctccatc atcgctaggg ctgtttcaag
     7861 gactttttca ccgttcataa gaccataatc agccatgtta atggcatcta ctttaatatc
     7921 tggttcaaac ttatctttaa aatcctttag taagaatttg acttgtggac ctaacaagag
     7981 aacgtcaatg ctattagcag ccacttcgtt gtcagcttca ctcacaggaa ctgcccaaat
     8041 agaaacttca aggtcacgcg cttgggctgc tttttgcatc ttagtcacca acatactagt
     8101 tgacataccg gcattacaaa ctaacataat agatttcata aggtttctcc ttttaatcgt
     8161 tttaatcatg aataagtggg gtaaacactg tcatgaattt ggaataattt ggttcttcta
     8221 gtaacgtttg ctgaagctcc acctgattga caaaagatgc caaacctggt gatacatatt
     8281 ttaacctatg attatgccct ttagacgggg ataatagaaa gacaaaatga acagcttgat
     8341 gcaccttgtc ccactcaaga ggctctcggc aaatagccac tactacctgt tcagaataag
     8401 tcatgggatt agcaggatgc ggaaaggcaa gcacttctcc atagatgact gggctgtaat
     8461 tttccctcag taccatttga tgatagaagt cctctacaaa catttctgtt cccgcctcgt
     8521 caagacaagc aatcatccgt aaaagcaagt cttcctttga gattttctcg tctaaataca
     8581 gaaaacgatt agggctaaaa acacctgtta gaagttgctc tgcttttgct aaactcatct
     8641 gtgacggtaa actgacctct ttcttgatac cttcttgttc tccaataaat tgtcgaatag
     8701 tttcaatatc ctgatttgac aaaaaaacac tgacagtaat cactggtgtt aaaaacatca
     8761 gattggcaag gctgatagag gaaataatca aatcaacggt tttcagtcgc tcttcggtta
     8821 tttcatagta actgataaca tcgacaatac gcaaacgtcc ttcaaattcc ttttccaagc
     8881 gatttttcaa catcatggca ctaccataac ctgtggcaca aactactaga accctcaatt
     8941 tatgacggtt tgaataacgc tcaatggccg ccattaaatg caaactaata taagcccatt
     9001 catcatctga gacatcattc ttttgacata caagcaaatc cgaaaaggtc tgcttggtaa
     9061 gggtaaatat ttcaggatac tggcttttaa tttcctcagt taaaggatta gtcagctgaa
     9121 tatggttttc aagacgtgtg gtcaagggca tcatgtgggc taataaacct tgaaataaat
     9181 tggtatctgt ttctaaagtc atgcctgtca attgactaat cttggcaata catgctttta
     9241 aatggtcctg caacttttca tcggtattat catcttgcca atgcttacca acagaatgtt
     9301 tgacctttag atgcagtgca atataattag cctcctcttt aggaaaacgt atccccatca
     9361 cttcttccac tcgatacaaa atgcgcagag ctacttgata ctcatcagac tgacgaatag
     9421 ctattggaat ggaaaacagt tctaatggac accccgatcg aatacgctga accattaagg
     9481 caatatgcaa gactagatta ttcatgacaa aatcagaaag tttcaactta gcatcccgac
     9541 attcatccaa gacaataatc atgatttctg caaaattaat accttcaaga aagatattac
     9601 ctaccatggt ggacaaggta ttatcaaaac tagtaacaaa aaaatagtcc atgataaaat
     9661 gcctaatatc ctcttcttta cctcttattt gaatatgttg gtgagttatt tcaagctcta
     9721 actggtaagg tattatgcgc tccttaatgc ctgctaaaac atgttttaaa gttgtccgac
     9781 tgatatacaa ttcttgacaa agctcttcaa aatcctgaac ggcatcttca aaaaagaatt
     9841 tatgaagcac ataatcttct cgatcaaccg attcttccac ttgagacaca tctgccaaac
     9901 gttttttaga cgtcacactt tcttgccaaa agatgtcaaa tgccatagaa tggtcaattt
     9961 ctaagcagta acctcgacct tgtttagaaa tgatgtgagc tccgtgttca ggaaggctac
    10021 tgatcagttc tttgaggtat ttcctaaccg tacggtcact cataccaata acctcagcca
    10081 gagttgcact agagacaaac tgctctttgt gatggattag aaatgttatg atggccagtt
    10141 ctttttttga taacattagt cctccttact actttagaaa acgctttcta ccctctttta
    10201 gtgtaccaaa tatttcctga aaaaggtgac catcattttc cgcattgatt gtgaaaagaa
    10261 tctatgatgg gtcaatctcc aaacaaaatt cctgccaagg tcgcaaatca tctaacaaac
    10321 taagatagtc ctctctgacc tcagcaacca cattagcttt tgaactaacc gtaaaatttt
    10381 tcaaagcaat ttgcagttct cccttatatc gatgatagcg gtcattgtca ataataatgc
    10441 aacctcgttt gacctcctta gattggtctc ttggagcaat cgattcctga gcataaacta
    10501 gacgtggcat ggttgatcga atcacataat cagatacatc gccacgataa caatgcggat
    10561 agccaatgat ttgctcctca acttctgtca aatcgatgat aggcctaacc ttgatcgttg
    10621 tgactggtct tgctaaagcc tgagtacatg ctgccaattc ttcttccgaa atgaattggt
    10681 tagatatgag gatattatca atggttccta tcgctttcat taattctact tgcaggccaa
    10741 tgggcaagtg cctatggtct tccactgtcg gcaagccttc tgccagtagc caagggcctt
    10801 cagatgctga ctgagcggta ataaaagcag ccgtttcaat atcatgctca tgataaaaac
    10861 gtgacatatc cttaaaatgt tgccatgaca aaccggtaaa ttcatgtgga taaaaattat
    10921 ggcaaccaat aatattgctc cgttctgcat ctgtcgctag caatgacatc aataattgtt
    10981 tatcagtgct catattaagt tcaattttaa gcccaaaggg atttcttgtt aaagttacaa
    11041 tctctgctag agggagagcc tcgtctaagc gtaagcccgc taacccgaag gcatgagctc
    11101 gttcaataag ctgatctgac caaccagctt ggctaataaa gcttggacta acgtcagcaa
    11161 tcactcgaat gcctaattga tttgcataag caatgagctc tgcgtagcaa tgaaacatct
    11221 ggtgatcagc tggtgccagc tgtaagaggc tcataaacaa gcgcttggct ccatagctat
    11281 gacataagtc aatataagct ttactcttgg taacatcata ccgctcaggg tatagagaaa
    11341 atcctaaatc aaccatattg cctcctattt atcaaaacca tttgactcgc tcaacgcctt
    11401 aaaccagtaa cctgattttt ttatggttcg cttttgactc tctaaatcca aagctaccaa
    11461 accatatcgg tttttataag catttaacca agaccagcaa tcaataaaag tccacaagag
    11521 gtagcctttg cagttggctc cttctcccaa agccttatga agctggataa gatgatcctc
    11581 gataaaggtg atgcggtagt catcttgtat ctggccatct gctaaaaaag cttcttcacc
    11641 ttcgacaccc ataccatttt ccgtgactag ccattcgatg ttaccatagt gttcttttaa
    11701 attaagggca atatcataaa gtcctggttc ataaatttcc caaccccggt gaggattgac
    11761 ttttttacca ggcatatcat aaggttcaaa ataaatatct aagataaggg ggttcccctc
    11821 ttgctgtgac ttactaggtg cctgaacccg caaaggctga taataattaa ctcccaaaaa
    11881 atcaacagta ttgtttttga taatctctaa ttcatcagca ctatattgag gtaataaatc
    11941 agcttcctct aaaatggaaa ctaactctgc tgggtaaaca cccaaaactg atggatctag
    12001 gaaggatttg gtttggaaaa gctctgcgat tctagcagcc ttgacatctt caggagcatc
    12061 actccgagga taagcaggtg tcatgttaag cacaatacta atcttgtggt cgggatgaag
    12121 cttatgacaa gctttgaccg ccagtgaact ggccagttgt gtgttgtaag ccacttgaac
    12181 tgctgccttg gcatctactt tacaagggta gtgataataa cctaaatagc cacattcgac
    12241 tggaacaatc ggttcattaa aggtaatcca agtgtttact aaatcaccaa aaagctcaaa
    12301 acaggttttt gcgtaggttt cataagccca gacagtagct ttgttttccc agccaccttt
    12361 ttcttgcaag gcatagggga ggtcaaaatg atagaggtta acaatcaatt taataccttg
    12421 agcaataatg tcttgaaata cttctcggta aaatgtcacc gcctttggat taacctcccc
    12481 gactccttct ggaatcaatc gagaccactg gattgaggtc cgaaaaatcg tgtggcctgt
    12541 ttctttcaaa agggcaatgt cacccttata gttttcataa aaggttgagg ttttttctgg
    12601 accaatttgg tgatgaaact tagctgactc aatactaaac cagtaatccc agttgctagg
    12661 tcctttgcca tctccaggaa ccgttccttc gctttgaggc ccagatgtgg aacttcccca
    12721 taaaaagcca tctggaaatt gataacggtg ttttctttgt ttcatatcct ttgtcctttc
    12781 tttgctcact tctcttttta ttataagaag aaaaacaggt gatgtaaacg ctttaaaaaa
    12841 tctgtttttt ccttatcatc acggaaaata agtgatttaa agcttgacat attttattat
    12901 cttaaagagc ctatctaacc tgccctgcta cttcttatca aaaccaagcc cctattgaca
    12961 gatgccttag cctatgtttg aataggggta tggcttattt aaactacttt acaaaaaccg
    13021 agtgggcact ttggttaagc tctgtcttgg ccattttaat tagcgccctt ttattcggta
    13081 agcaagctcc cttagcttta atagcttctt tgattggtgt gacctctctc atttttagtg
    13141 ctaaagctaa tcctattggt caagggctcg tcattatttt ttccattatc tatgcctatc
    13201 tttcattacg aaacagttac tatggagagt taatgaccta tcttttcatg accctcccta
    13261 tgactatttt ttcacttttt acttggttaa accatccttt tgaagggaaa aaatcacaag
    13321 tgactatttc acgattaaca cccactgatc gaagatgtct tttcgttttt actatactgg
    13381 tcactcttat tttctacggc atcttggctt ctattcaaac agcctattta cttgtctcca
    13441 ctttatcgat tgccacttct ttctcagccg tttatctcag ctataagcga agcccttatt
    13501 ttgctttggc ttatggactc aatgatctag tcctcattct cttatggatg catgctgctc
    13561 aaacagatgc cagccaatat gctgtggtca tctgctttgc cacctttctt attaatgata
    13621 gttacacttt ttatagttgg ctaatgctcc aacgttacca agaaaaaaag gtcaaaaaag
    13681 gatgagggcc taagcctcat cctttttcaa tttttcacgt tctaagcgca aacgtctatt
    13741 gggatttaaa agattaaaca attcttctaa tttctcagca ttccaaacat ctgctgccga
    13801 tacaaaattt ccatccttat ccttaaagga tatcggttta tcacccataa tcagaaacct
    13861 ccatcttaaa gataaaacac aaggctgcta tggcaatgcc atttctaaag accacattaa
    13921 tccacaaatt caaattcaaa tttaccaata cgaacaagat ccccgtcttt tgctccacgt
    13981 tcacgaaggg cttcgtcaac ccccataccg cgcaattgac gggcaaattt catgatggat
    14041 tcgtcacgtt ccatattggt catgacaaag agacgttcta acttctcacc agacaagacc
    14101 caagtagcgt catcgtcacg tgtgatctca aattctttct cagcctcagc aaaaccatag
    14161 taagcttcct cgtccaccag atcagattca tcatacaata agaattcatc cgtcttagca
    14221 agtaattctg ccgttgcttc taacaagttc tctaagcctt gatgtgctag gcttgaaata
    14281 gggaaaatca ttggcaaatc atcaaactcg tcatattggg cagccaattt tttcttgaag
    14341 gctttcaagt tttcttgagc ctctggcatg tccattttat tagccacaat aatctgtggg
    14401 cgctccatca atcgcaaatt ataggtttcc agctcgttat taatagaaac ataatcttcg
    14461 taaggatcac gaccctcact agcagacatg tcaatcacat gaagaataac ccgtgtccgc
    14521 tcgatatgac ggaggaattg agttcccaaa ccaacccctt ggctagcacc ttcaatcaaa
    14581 cctggcagat ctgccatagc aaagctatca cctgactttg tacgaaccat tccaagatta
    14641 ggaacaatag tggtaaaatg ataggcacca attttcggtt tagctgatga cacaacactt
    14701 aacaaggttg atttcccaac agatgggaat ccgaccaagc caacgtcagc taagattttg
    14761 agttctaact ctaactgacg ttcctcacct ggctcaccat tttcagcaat ctctggcgca
    14821 ggattacgag gagttgcaaa acggatattc ccacggccac cacgaccacc cttagcaatg
    14881 accacttctt gaccatgttc tactaagtct gtaattactt ttccagtttc agcatcacga
    14941 accgttgtcc cttgaggaac aaaaacaatc aaatcttctg caccacggcc gtgcatgcct
    15001 ttggtcatac ctttttcacc agatttcgcc ttgaatttac gattatagcg gaaatccatc
    15061 aaggtacgca aaccttcgtc cactcggaag ataactgagc cacctttacc gccgtctcct
    15121 ccccaaggac cgccatttgg gacgtacttt tcacgacgaa aagctaccat accatcgcca
    15181 ccacggcctg cttggacact gatttttgcc gtatctaaaa acatactcat acttttttta
    15241 ttctctaact ttcttttact ttaaaaaaac gcctttcagc gtttccttac attaatactg
    15301 atagggcact cgcaataagc ccgccaacag ttacaagaac cattaggata acaacgacca
    15361 ttgtcacttt ttcaaaggtt gtttttttgc gaggtccatt ttctccaaat gccacgttaa
    15421 atacctcatc tattgatttt tcttcttata tcttaacatg aaagatcgtt ttaggcaaat
    15481 accagatgaa tatttacgct atgtaggcta ttactgagta ttgtatcttg tgaatttcat
    15541 atgaatatga tacattagca tttgcaaaat aatgttacag ataaaaacct cctcgtctg
//
LOCUS       DATURV010000035        15543 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DATURV010000035 DATURV010000000
VERSION     DATURV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.035"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(17..643)
                     /locus_tag="VCF75_001093"
     CDS             complement(17..643)
                     /locus_tag="VCF75_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEP2546660.1"
                     /translation="MTYDKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRL
                     HQAAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTW
                     QFDQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDN
                     YSQASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
     gene            complement(938..1285)
                     /gene="ssrA"
                     /locus_tag="VCF75_001094"
     tmRNA           complement(938..1285)
                     /gene="ssrA"
                     /locus_tag="VCF75_001094"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1377..1991
                     /locus_tag="VCF75_001095"
     CDS             1377..1991
                     /locus_tag="VCF75_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEP2546661.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            complement(2122..2907)
                     /locus_tag="VCF75_001096"
     CDS             complement(2122..2907)
                     /locus_tag="VCF75_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546662.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNIN
                     TSADSFPFTEQKTIYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(2917..3615)
                     /locus_tag="VCF75_001097"
     CDS             complement(2917..3615)
                     /locus_tag="VCF75_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546663.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(3615..3986)
                     /locus_tag="VCF75_001098"
     CDS             complement(3615..3986)
                     /locus_tag="VCF75_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2546664.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            4171..7281
                     /locus_tag="VCF75_001099"
     CDS             4171..7281
                     /locus_tag="VCF75_001099"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEP2546665.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELTDCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQELTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKIMDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            7361..8374
                     /gene="pfkA"
                     /locus_tag="VCF75_001100"
     CDS             7361..8374
                     /gene="pfkA"
                     /locus_tag="VCF75_001100"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEP2546666.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            8437..9939
                     /gene="pyk"
                     /locus_tag="VCF75_001101"
     CDS             8437..9939
                     /gene="pyk"
                     /locus_tag="VCF75_001101"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEP2546667.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            10157..10714
                     /gene="lepB"
                     /locus_tag="VCF75_001102"
     CDS             10157..10714
                     /gene="lepB"
                     /locus_tag="VCF75_001102"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP2546668.1"
                     /translation="MVKRDFIRNILLLLIVIIGAILLRIFVFSTFKVSPETANTYLKS
                     GDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYL
                     EKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLI
                     NASQIKGLVTFRVLPLSDFGFVEVE"
     gene            10890..12704
                     /gene="glmS"
                     /locus_tag="VCF75_001103"
     CDS             10890..12704
                     /gene="glmS"
                     /locus_tag="VCF75_001103"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEP2546669.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSRESTYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLTTTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            12900..13235
                     /locus_tag="VCF75_001104"
     CDS             12900..13235
                     /locus_tag="VCF75_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEP2546670.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            13342..13983
                     /locus_tag="VCF75_001105"
     CDS             13342..13983
                     /locus_tag="VCF75_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP2546671.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            13993..14622
                     /locus_tag="VCF75_001106"
     CDS             13993..14622
                     /locus_tag="VCF75_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2546672.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQNVDAKEKALDLLTRLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            14638..15474
                     /locus_tag="VCF75_001107"
     CDS             14638..15474
                     /locus_tag="VCF75_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP2546673.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     ALLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQSGKFQAISEKWFGDDVATANI
                     KS"
BASE COUNT         4875 a         2548 c         3179 g         4941 t
ORIGIN      
        1 atatattacg cattttttat tcttcaagtc ctacctgaga catttctgtt aactgattat
       61 tttcaaaaac gagagaaata ttagctccgc ttgtatctgt ctttaagcca ctaacccaaa
      121 tcgcttgcaa agtttgatga tcactagatg acgcttggct ataattatca ggttctgtta
      181 agatcttttt aacgtcttca taagacattc ctttttgtaa ttgttggtat tccttttgcg
      241 ataagcctaa ctctcttgca aaggtaaaat tagagatggt tttaacaata ctactatttt
      301 gataaagatt aacagtgaga gtaacttgat caaactgcca agtataagcg tcgattgtta
      361 cttcgcctgc tgtttttgga tcatgttggc taggctctcc aaagagtgaa ataagttctt
      421 caaggcttgt tcctcctgta aatgaggaat caacagtcgc aagtttaatt ttttcaaagt
      481 tagcgcgttt atcttgatga gcggcttggt gtagtcgggg agtaatgatt tctgaatcag
      541 acggttgagg ctctttatct ttcgttgttg aacaagctac caaaaagcta gtaaaaaaag
      601 agatgagaag aagtgttagt aagtgttttt tgtcatatgt tatttcctaa actatttttg
      661 ctgtattata tcataaatta taaacaagat atagctatga ttccccataa cgcatgtgct
      721 atggggaatt tagtactatt ttgattcttt tttttagata ttcatgaaaa aaatggtcaa
      781 aaagctctga tatgacaagg ttttgagtag tattgatgga gtatcggtca gcgtgatggt
      841 atggctatag ggataacgga aactcatgaa caagacaaaa agaaaaaacc ttgatgtaac
      901 aaggttttag taagttatga ttacttacgg taagcattga tggagccggt gggagtcgaa
      961 cccacgtcca aacacctgcc aacatatttg tctacgtcca taggttatgt cttcatttaa
     1021 caaactaaag acacataact caagccatta gtttgcgagt ctatcaatct cttgtcacga
     1081 aactagacaa tttcgtaacc gtagcttgct attttgagac ttctaacagg acacaagcaa
     1141 tcttgtagaa gtcacgctag ctggttttta ggcagctaaa gcgtaagagt ttgtattttt
     1201 tgcagttata tttaactggc aatttacatc tgccgatggg acgcaaaaca tgcctcataa
     1261 tgcctgtcga atccgtaaca accccaaata gataattaac caatttctta cactaactat
     1321 tctatcaaaa ctttttttaa atagcaaaat tttacagctt atggtatgat tagtctatga
     1381 caaaacgtaa ttcaaaagcc tatatattat ggcaaaaaat tatcaaaatt ttaggaatta
     1441 ttgcattaat tggtactttt ttcctcgctt tttggctgta tagactaggt atattaaacg
     1501 atagtaatgc tcttaaagac ttagtgcagc gctatagatt atgggggccg tttgttttta
     1561 ttgtcgtgca gataatacag attgtttttc ctgtgattcc tggaggcctt acgacagttg
     1621 caggtttttt aatctttggc cctgtaacag gttttattta taattatgta ggcatcatta
     1681 ttggcagtat agtcctcttc ttgcttgtta agacttatgg tcgtaaattt atcttactct
     1741 ttgtaaatga caaaacattt tacaagtatg aaaggcgttt ggagacacca ggatatgaaa
     1801 aattatttat tttttgtatg gcatcgcctg tttctccagc tgatattatg gttatgatta
     1861 cgggattgac ggacatgtct ttaaagcgct ttgtaactat tcttttaata actaaaccaa
     1921 tttctattat cggctacagt tatttattta tttttggaaa agatgttata tcttggttct
     1981 tacagcatta aggtttggtg ttaatttgat aaaccgcttc tatttgatgg aagacggttt
     2041 ttttatttac tatgaacact gaaaaaaact ttcttttaaa ctatagtgtg tcatagttta
     2101 aaagaaagtc ttaacgctta cttactgtag gttgagttta tttttgataa taaaattagt
     2161 ggcgaggtaa aacattatca tttcaataaa ttgttccaaa ataagaagat aaattgtttt
     2221 ttgctcagta aatgggaaac tatcggcact tgtattaata ttaaagatgt gactgtgaac
     2281 atatgttgca gcaacgctaa tcagaataac taatataaaa tatgcaataa aggccatcaa
     2341 gcctcgccga ttagaaaaaa gttgacctat agaaatggaa agataaatta ataagatact
     2401 cgtaaatgtt gataatagga cataggctag tacagttagc atattgatga aataatccat
     2461 cttaaaacta ttaaagaatc cttctaatag ttcgttgatg ttaaacattg gtacaattac
     2521 aatagcgata gcaaatgcaa ggatgatagt attgaaaaca ctgcaaataa aagaagctag
     2581 tagttttgat aaaataattt gatgcgaatt aacgggtaaa gtcaacgtta aatatccttc
     2641 ccgtccaaaa acacttttgc taaaacgttt gataatgata agtaatgttg ataagaggga
     2701 accagcgatc agggagccaa aagttaaacc taatgtcaga ggcaacatct tattggttag
     2761 tactccaaat agtccatcac tattgctttg agcaaacagt tttatcgtaa atgacaaaat
     2821 ggcagcgatg gcaataacga aagcattaag cgcaaagtac cattttccaa tagatctaaa
     2881 ttcgtatttt aataattttc caaacatgat atcctcctaa accttataag tttccctaaa
     2941 gagggaatca atggattgct ggtattcttg tcttagatca tcagcattgc cagataagaa
     3001 aagtcttcct tgtttcaaga aaatgacttc gtcaaggata ggttcaatgt ccgaaatcaa
     3061 gtgggttgag ataataacag aagcattttc acaataacta ttaatgatgg tttttaggat
     3121 gtagtctctt gcggcagggt caactccacc aataggttca tcaagtacat agagtctggc
     3181 tttacgactc atcacaagaa ttaattgtac tttttctttg tttcctttgg atagagtttt
     3241 gaagcggtct tcagggtcta gttctaaatc tcttagaagg cttgttgctt tggatttgtc
     3301 aaagtcgctg taaaaatcac taaagaactc caacatgtcc ttaattctca tattttcatt
     3361 gagataagta gtatctggta aataagaaat aatttttttt gtctcaacac agggacggta
     3421 gccatcaatg acaatttctc ctttatttgg ttgtaaaagg ccattaatga gtttaattaa
     3481 agttgtttta ccacttccat ttggacctaa taatccgata attttaccgt tcgggatagt
     3541 aatggttaag tcatcaatag cttttttttc acggtaagat tttgaaacat gatgtagttg
     3601 caataggtgt gccattattg tgaatcctcc tttaaaaagc tcgtcaaaac agggataatc
     3661 tcagtgtgac taaatcccat ttttgtcata ttggtgataa atgattctaa ttcactaatt
     3721 gctaactctc gtcttttacg agcaattaat ttttgatcgt cagttacaaa acggccagca
     3781 gtacgttgcg aataaaccat tccttcccgt tcaagttctg taaacgctcg ttgcatggtg
     3841 ttaggattga cacctgcgat ttctgcatat tcacgaacag ttggaagttg atcaccactc
     3901 ttgatttctt ggctaataat ttgcatcatg acatgttgtg ctatctgcgc ataaatagga
     3961 gatttttcat caaatttcca agacatgtgg tctcctttct gagtaatata gatatttgta
     4021 ctaattaaat ggtacagaaa aaaaagggga atgtcaagtt ttatgccatt tttcactctt
     4081 tgagaagtag acgtagatta gagagaaaat tgaagcaaga aaaccttttt catagtataa
     4141 tagggaacaa gataaccaaa ggaggaactt atgtttgctc aacttgatac taaaactgta
     4201 tactcattta tggatagttt aattgactta aatcattatt ttgaacgagc aaagcaattt
     4261 ggttaccaca ccataggaat catggataag gataatcttt atggtgctta ccattttatt
     4321 aaaggttgtc aaaaaaatgg actgcagcca gttttaggtt tggaagtaga gattctctat
     4381 caagagcggc aggtgctcct taacttaatc gcccagaata cacaaggcta tcatcagctt
     4441 ttaaaaattt ccacggcaaa aatgtctggc aagcttcata tggattacct ctgccaacat
     4501 ttggaaggga tagcggttat tattcctagt aagggttgga gcgatacatt agtggtccct
     4561 tttgactact atatcggtgt tgatcagtat actgatttat ctcatatgga ttctaagagg
     4621 cagcttatac ccctaaggac agttcgttat tttgcgcaag atgatatgga aaccctgcac
     4681 atgttgcatg ccattcgaga taacctcagt ctggcagaga cccctgtggt agaaagtgat
     4741 caagagttaa cagattgtca acaactaacc accttctatc aaacacactg ccctcaagct
     4801 ctacagaatt tagaagactt agtgtcagga atctattatg atttcgatac aaatttaaaa
     4861 ttgcctcatt ttaatagaga taagtctgcc aagcaagaat tgcaagagtt gactgaggct
     4921 ggtttgaagg aaaaaggatt gtggaaagag ccttatcaat cgcgcttact gcatgaattg
     4981 gtcattattt ctgacatggg ctttgatgat tattttttga ttgtgtggga tttacttcgc
     5041 tttggacgca gtaaaggcta ttatatgggt atgggacgtg gctcggcggc aggtagtcta
     5101 gtggcttatg ctctgaacat tacagggatt gatccagttc aacatgattt gctatttgag
     5161 cgctttttaa acaaagaacg ttatagcatg cctgatattg atatcgatct tccagatatt
     5221 taccgttcag aatttctacg gtatgtccga aatcgttatg gtagcgacca ttcggcgcaa
     5281 attgtgacct tttcaacctt tggacctaaa caggctattc gtgatgtttt caaacgattc
     5341 ggggttccag aatacgaact gaccaatctc actaaaaaaa ttggttttaa agatagcttg
     5401 gctactgtct atgaaaagtc aatctctttt aggcaggtta ttaatagtag aactgaattt
     5461 caaaaggctt ttgccattgc caagcgtatc gaaggaaatc caagacaaac gtccattcat
     5521 gcagctggta ttgtgatgag tgatgatacc ttgaccaatc atattcctct aaaatcgggc
     5581 gatgacatga tgatcaccca gtatgatgct catgcggtcg aagctaatgg cctgttaaaa
     5641 atggattttt tggggttaag aaatttgacc tttgttcaaa aaatgcaaga gaaggttgct
     5701 aaagactacg ggtgtcagat tgatattgca gccattgatt tagaagaccc gcaaacgttg
     5761 gcactttttg ctaaagggga taccaaggga attttccaat ttgaacaaaa tggtgctatt
     5821 aatcttttaa aacggattaa gccacaacgt tttgaagaaa ttgttgccac taccagtcta
     5881 aatagaccag gggcaagtga ctataccact aatttcatta aacgaagaga aggacaagaa
     5941 aaaattgatt tgattgatcc tgtgattgct cccattttag agccaactta cggtattatg
     6001 ctttatcaag aacaagttat gcagattgca caggtttatg ctggttttac gttaggcaag
     6061 gccgacttgt taaggcgtgc catgtctaaa aaaaatctac aagaaatgca aaaaatggaa
     6121 gaagacttta ttgcttctgc taagcaccta gggagagctg aagaaacagc tagaggactt
     6181 tttaaacgga tggaaaaatt tgcaggttat ggttttaacc gcagccatgc ctttgcctat
     6241 tcagctttag cttttcaatt ggcttatttc aaagcccatt acccggctgt tttttacgat
     6301 atcatgatga attattctag cagtgactat atcacagatg ctctagaatc agattttcaa
     6361 gtagcgcaag ttaccattaa tagtattcct tacactgata aaattgaagc tagcaagatt
     6421 tacatggggc tgaaaaatat taaggggttg ccaagggatt ttgcttattg gattattgag
     6481 caaagaccat ttaatagcgt agaggatttt ctcactagaa ctccagaaaa atatcaaaaa
     6541 aaggttttcc ttgagcctct gataaaaata ggtctgtttg attgctttga gcctaaccgt
     6601 aaaaaaatta tggacaattt ggatggttta ctggtatttg ttaatgagct tggttctctt
     6661 ttttcagatt cgtcctttag ttgggtagat acgaaagatt actcagcaac tgaaaaatat
     6721 tctttggaac aggagatcgt tggggttggc atgagcaagc atcctttaat tgatattgct
     6781 gagaaaagta cccaaacttt tactcccatt tcacagttag tcaaagaaag cgaagcagtc
     6841 gtactgattc aaatagatag cattaggatc attagaacca aaacaagtgg gcagcaaatg
     6901 gcttttttaa gtgtgaatga cactaagaaa aagctcgatg tcacactttt tccacaagag
     6961 tatgccattt ataaagacca attaaaagaa ggagaattct attacttaaa aggtagaata
     7021 aaagaaagag accatcgact gcagatggtg tgtcagcaag tgcaaatggc tattagtcaa
     7081 aaatattggt tattagttga aaaccatcag tttgattccc aaatttctga gattttaggt
     7141 gcctttccag gaacgactcc agttgttatt cactatcaaa aaaataagga aacaattgca
     7201 ttaactaaga ttcaggttca tgtaacagag aatttaaagg aaaaacttcg tccttttgtt
     7261 ctgaaaacgg tttttcgata aaaatggaca attagtgaag tattttgcaa atgaatgtgc
     7321 tataataaga ccgttaaatt aattaaagga gtaccagaaa atgaaacgta ttgctgtttt
     7381 aaccagtggc ggcgacgctc ctggtatgaa tgctgctatt cgtgcagtcg ttcgtaaagc
     7441 aatttcagaa ggaatggaag tttatggtat caaccgtggc tatgcaggta tggttgatgg
     7501 cgacattttc ccactgggtt caaaagaagt tggtgataaa atctcacgtg gtggaacttt
     7561 cttatactca gctcgttatc cagaatttgc tcaacttgaa ggtcaattgg ctggtattga
     7621 gcaacttaaa aaacatggta ttgagggtgt tgttgttatt ggtggggatg gttcttatca
     7681 tggcgctatg cgcttgacag agcacggctt cccagcggta ggtatcccag gaacaatcga
     7741 taacgatatt gcgggaactg actacactat cggatttgat acagcagtta acacagcagt
     7801 tgaagctatt gataaacttc gtgacacctc atcaagtcac ggacgtactt ttgtggttga
     7861 agtgatgggt cgcaatgcgg gtgatattgc tctttgggca ggtattgcta gtggtgctga
     7921 tcaaatcatt gttccagaag aagagtttga tattgaaaaa gtggcatcaa caatccaata
     7981 tgattttgaa cacaaaggta aaaatcacca cattatcgtc ttagctgaag gtgttatgag
     8041 cggtgaagct tttgctcaaa aactgaaaga agctggcgat aagagtgacc ttcgtgtgac
     8101 aaatcttggt cacattcttc gtgggggttc accaactgct cgtgaccgtg tcattgcttc
     8161 ttggatgggt tcacatgctg ttgagctact taaagaaggc aaaggtggtt tggctgttgg
     8221 tattcacaat gaagaacttg ttgaaagccc aatccttggt actgcagaag aaggtgcttt
     8281 gttcagcttg actgaagaag gcaagattat tgttaataat ccacacaaag ctcgtttaga
     8341 ctttgcagct ttgaatcgtt cattgtcaca ataagtttag ttaaaattgt taaatcgtca
     8401 attattgaca aaaaaataag ggagtttcag agtaaaatga ataaacgcgt aaaaatcgtt
     8461 gcaacacttg gtcctgcggt tgaaatccgt ggtggtaaaa aatacggaga agatggctac
     8521 tgggctggtc aacttgatgt tgaagaatca gcaaaaaaaa tcgctgaatt aattgaagct
     8581 ggtgcgaatg ttttccgttt caatttctca catggtgacc acaaagaaca aggtgaccgt
     8641 atggcaactg ttcgccttgc agaagaaatt gctcgtcaaa aagttggttt ccttttggat
     8701 actaaaggtc ctgaaatgcg tacggaattg tttgcagatg atgctaaaga gtttagctat
     8761 gttactggag aaaaaatccg tgtagcgact acacaaggta ttcaatcaac tcgcgatgtt
     8821 attgctttga acgttgctgg tagccttgac atctacgatg aagtagaagt aggtcatact
     8881 attcttatcg atgatggtaa acttggtttg aaagtgatcg acaaagatat cgcaacacgt
     8941 caatttatcg ttgaagtaga aaacgacggt attattgcta aacaaaaagg tgttaatatc
     9001 cctaacacta aaattccttt cccagcactt gcggaacgcg acaatgctga tatccgtttt
     9061 ggtcttgaac aaggacttaa ctttattgca atctcatttg ttcgtacagc caaagacgtt
     9121 gaggaagttc gtgaaatctg tcgcgaaact ggtaacgacc atgtgcaatt atttgctaag
     9181 attgaaaacc aacaaggtat cgataatctt gatgaaatca tcgaagctgc tgatggtatc
     9241 atgattgcac gtggcgacat gggtatcgaa gtaccatttg aaatggttcc agttttccaa
     9301 aaaatgatta tcactaaagt gaatgcagct ggtaaagcag ttatcacagc aacaaacatg
     9361 cttgaaacaa tgactgaaaa accacgtgcg acacgttctg aagtatctga cgtcttcaat
     9421 gctgttatcg atggtactga tgcaacaatg ctttcaggag agtcagctaa cggtaaatac
     9481 ccggttgaat ctgttcgtac aatggcgact attgatcgta atgcacaaac tcttcttaac
     9541 gaatatggtc gtttagattc atctgccttc ccacgtacca ataaaacaga tgttattgct
     9601 tcagctgtta aagatgccac tcattcaatg gacatcaaac ttgttgtgac tattactgag
     9661 acaggtaata cagctcgtgc gatttctaaa ttccgtccag atgcggatat cttagctgta
     9721 acatttgatg aaaaagtaca acgtgctttg atgattaact ggggtgttat tcctgttctt
     9781 gctgaaaaac ctgcatctac tgatgacatg tttgaagtag cagagcgtgt tgctgtggag
     9841 gcaggcttag ttcaatctgg ggataatatt gttattgttg ctggtgttcc agttggtact
     9901 ggtggaacga atactatgcg tgttcgtaca gtaaaataaa tgtgttataa ctatttacct
     9961 aaaggttaga aggcaagctt ctaacctttt ttgatatcaa aaatatcatg ttaattcact
    10021 agtgttaatt tactttaatg agtctaagtt tgtattttat acagaatgag caacgtttat
    10081 tggtattgat caaaaattgt ttactttttt tctagcttag aacatggtat aataagacaa
    10141 tagactagga gttaatatgg taaagcgaga ttttataaga aatatattat tgttattaat
    10201 tgtgattatt ggggctattt tgttgagaat ctttgttttt tcaactttta aagtttctcc
    10261 agaaacagct aatacttatt taaagagtgg tgatttagtc acaatcaaaa aaaatattca
    10321 gcccaaatat aaagattttg tggtttatag agttggaaaa aaagattatg tcagtcgagt
    10381 cattgctgtt gaaggcgata gcgtaactta tatggatgat attttttacc tcaataatat
    10441 ggtagagtca caggcttacc ttgaaaagat gaaagcacat tacttgaatc atgcaccgtt
    10501 tggcacattg tatacagatg attttacagt tgctaccatc acagctgata agtatcaaaa
    10561 agttcctaag gggaagtatc ttcttttgaa tgataaccgg aaaaatacga atgacagtcg
    10621 tcgatttggg ttaataaatg cctcgcagat taaaggttta gtgaccttta gagttttgcc
    10681 tctcagcgat tttggatttg tagaagtaga gtagttgtca ggctactcta ctttttttat
    10741 aattagacta taccaatttt atgtttgaca agacattgtg gatattatat actgtaaaca
    10801 tactgttttt atcggtataa ttagactata ccaattattg aaattatgag agaaagtagc
    10861 aagttaacga acttgttaag gtaattaaga tgtgtggaat tgttggagtt gttggaaatc
    10921 gcaatgcaac ggatatttta atgcaaggcc ttgaaaagct tgaataccgg ggttatgatt
    10981 cagcaggaat ttttgtggct aatgccaatc aaacaaactt gattaaatca gtggggcgga
    11041 ttgctgattt gcgtgccaag attggcattg atgttgctgg ttcaacaggg attggtcaca
    11101 cccgttgggc aacgcatggc caatcaacag aggataatgc ccatcctcac acgtcacaaa
    11161 ctggacgttt tgtacttgtt cataatggtg tgattgaaaa ttaccttcac attaaaacag
    11221 agttcctagc tggacatgat tttaaggggc agacagatac tgagattgca gtacacttga
    11281 ttggaaaatt tgtggaagaa gacaagttgt cagtactgga agcttttaaa aaagctttaa
    11341 gcattattga aggttcctat gcctttgcgt taatggatag ccaagcaact gatactattt
    11401 atgtggctaa aaacaaatct ccattgttga ttggacttgg tgaaggttac aacatggttt
    11461 gttcagatgc catggccatg attcgtgaaa ccagtgaatt tatggaaatt catgataagg
    11521 agctagttat tttaaccaaa gataaggtaa ctgttacaga ctacgatggt aaagagctga
    11581 tacgagattc ctacactgct gaattagact tatctgatat tggcaaaggg acttatcctt
    11641 tctatatgct gaaagaaatt gatgagcaac caaccgtaat gcgtcaatta atttcaactt
    11701 atgcagatga aactggtaac gtacaggttg atccagctat cattacctct atccaagagg
    11761 ctgaccgtct ttatatttta gcggcaggga cttcctacca tgctggtttt gcaacaaaaa
    11821 atatgcttga gcaattgaca gatacaccag ttgagttggg agtggcttct gagtggggtt
    11881 accacatgcc tctgcttagc aagaaaccaa tgtttattct actaagccaa tcaggagaaa
    11941 ccgcagatag tcgtcaagtt ttagtaaagg caaatgctat gggcattccg agtttgacag
    12001 taactaacgt tccaggatca accttatcac gtgaatcaac atacaccatg ttgattcatg
    12061 ctggacctga aattgctgtt gcatctacaa aagcttacac tgcacaaatt gctgcccttg
    12121 cctttttggc taaggcagtt ggtgaggcaa atggtaagca agaagctctt gactttaact
    12181 tggtacatga gttgtcattg gttgcccaat ctattgaggc gactttgtct gaaaaagatc
    12241 tcgtggcaga aaaggttcaa gctttgctaa ctactactcg taatgctttt tacatcgggc
    12301 gtggcaatga ttattacgtt gcgatggaag ctgctttgaa attaaaagag atttcttata
    12361 ttcaatgcga aggctttgcg gctggtgaat tgaaacatgg aaccatttca ttaattgagg
    12421 aggacacgcc agtaatcgct ttaatatcgt ctagtcagtt ggttgcctct catacgcgtg
    12481 gtaatattca agaagttgct gcccgtgggg ctcatgtttt aacagttgtg gaagaagggc
    12541 ttgaccgtga gggagatgac attattgtca ataaggttca tcctttccta gccccgattg
    12601 ctatggtcat tccaactcaa ctgattgctt actacgcttc attacaacgt ggacttgatg
    12661 ttgataagcc acgtaatttg gctaaagctg taacagtaga ataaatgatt ataatggata
    12721 aggcaaccat ctgttaggtg gttgcttttt gtttttagga gaatagcaga gcttagaaaa
    12781 tggccttgag aaaagctata caagaagaat agtcatcttc tgatgagaac gtattcatct
    12841 tgtcaaatca ttgattctaa agtaaaatgg taacattaag aaatgtttag gagtgtaaga
    12901 tgtcattacc aaattgtttg caatgccagt ctgaatatgt ttacgaagat ggtcttttat
    12961 taatctgtcc aatgtgtgct tttgaatgga caccaggcga agaagcgact gaagaagaag
    13021 gacttgttgt tttagatagc aatggtgtcc gactgtctga tggtgatacc attactgtgg
    13081 ttaaagattt gaaagtaaaa ggcgctccaa aagacctgaa acaagggact cgtgttaaaa
    13141 atattcgcct ggtggaaggt gatcataaca ttgattgtaa gattgatggg tttggcgcga
    13201 tgaaattgaa atcagaattt gtcaagaaaa tctaataata atacgcaacc agctgatctg
    13261 gttgtatttt tataaaaaaa cctctcccca aatacgttca aatttggtat aatagtaaaa
    13321 tattcagttt taggaggcaa catgacctat attcagcaag ttttacccag cttattagat
    13381 ggtgccttgg tgaccttaca agtattcttt attgttatca ttctttctat acccttaggg
    13441 gctattttag ctttcttgat gaagattccc tttaaaccgc tacagtggtt tttgacccta
    13501 tacgtgtgga tgatgcgagg gacaccatta ctacttcaat tgattttttt ctattatgtt
    13561 ttgccaagtg tggggattag ttttgatcga atgccagctg ctattttggc gtttactttg
    13621 aattatgctg cctactttgc tgaaattttt agaggtggga ttgaggctat tccaaaaggt
    13681 caatatgaag cagctaaagt attaaagtta aaacctcttc aaaccattcg ttatattatt
    13741 ttgcctcaag tgtttaaaat tgtgttacca agtgttttca atgaagtcat taatttggtc
    13801 aaagattctt cccttgtcta tgtactcggt gtaggagatc ttttattagc aagtaagacg
    13861 gcagccaata gggatgcaac cttagcccct atgtttatcg ctggtcttat ctatttgctt
    13921 ttaattggac tggtcacgat tatttcaaaa caagttgaaa aacggtttaa ttattatcag
    13981 taaggaggct tcatgttaga actcaaaaat atttccaaac agtttggtca aaaaaccatt
    14041 tttgatggct ttaatctaac cgttcaagac ggagaagtgc tctctttggt aggtccatca
    14101 ggtggcggta agacaacact cttacggatg ttggctggtc ttgaatctat tgattcaggt
    14161 caagtctttt acaatggaga agatgtcggt attgatcatt tggaaaatcg taacttgctt
    14221 ggatttgttt tccaagattt tcaattgttt cctcatctta ccgttttaga caacctaacg
    14281 ctgtctccaa caattacaat gggcaagcaa aatgttgacg ctaaagaaaa ggctttggat
    14341 ttactgacac gtttaggatt aaaagaacat gcccaagttt atccctattc gttatctgga
    14401 ggtcaaaaac aacgggttgc tttggcgaga gccatgatga ttgatcctca aattatcggt
    14461 tacgatgaac cgaccagtgc cctagatcct gaattacgtc agacggtaga agccttgatt
    14521 gttcaaaacc gtgaaatggg aataactcaa attgtagtca ctcacgatct tgtttttgcc
    14581 gaagccatct cagatcgtat tattagggtt aatcctaaat agaagaggac ccaaaagatg
    14641 attataaaaa aaagaaccgt agcaatttta gccatagcta gtagcttttt cttggtagct
    14701 tgtcaagcta ctaaaagtct taaatcagga gatgcttggg gagtttacca aaagcaaaaa
    14761 agtattacag ttggttttga caatacgttt gttcctatgg gctataagga tgaaagcggc
    14821 aaatgcaaag gttttgatat tgatttggct aaagaagttt ttcaccaata tggactcaag
    14881 gttaactttc aagctattaa ttgggacatg aaagaagcag aactaaacaa tggtaaaatt
    14941 gatgtaatct ggaatggtta ttcaataact aaggagcgtc aggataaagt tgcctttact
    15001 gattcttaca tgagaaatga acaaattatt gttgtcaaaa aaagatctga tattaaaaca
    15061 atatcagata tgaaacataa agtgttagga gcacaatcag cttcatcagg ttatgacgcc
    15121 ttgttaagaa ctcctaaact gctgaaagat tttattaaaa ataaagacgc taatcaatat
    15181 gaaaccttta cacaagcttt tattgattta aaatcagatc gtatcgatgg aatattgatt
    15241 gacaaagtat atgccaatta ctatttagca aaagaagggc aattggagaa ttatcggatg
    15301 atcccaacga cctttgaaaa tgaagcattt tcggttggac ttagaaaaga agacaaaacg
    15361 ttgcaagcaa aaattaatcg tgctttcagg gtgctttatc aaagtggcaa atttcaagct
    15421 atttctgaga aatggtttgg agatgatgtt gccactgcca atattaaatc ttaaaaagga
    15481 cacttgtcct ttttttgttt aacaggtgag atctagcaga caaagtcagc tagacgattt
    15541 aat
//
LOCUS       DATURV010000036        15433 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DATURV010000036 DATURV010000000
VERSION     DATURV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15433
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.036"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            108..1109
                     /locus_tag="VCF75_001108"
     CDS             108..1109
                     /locus_tag="VCF75_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP2546674.1"
                     /translation="MKKTLKSTLILLGLTLAIGASTQVDASSRSWKSWFIEKYFWLKG
                     DKGYYSEQDETKFDQYVNSAGEASNKGFDLKEEQVNGTLVTKTVDNMQVHTWNENPSN
                     KANQKVIFYLHGGSYLNNPTTYHIDMLKTLSTSLDAKIILPIYPKAPTYSYSDAMPKL
                     VDLYRDTLKETTAQNITIMGDSAGGGLALGLALALKNESLKQPKDIVLLSPWLDVTMS
                     NPDIESYESNDPILSAWGLRKVGEIWARRDPNNTKHPYVSPINGDVSGLAPITMFVGT
                     HEIFYPDVKKFDDILTAKGHQHELIVADKMNHVYPIYPIEEAKTAQYQIIDTIQKAY"
     gene            complement(1162..1512)
                     /gene="rbfA"
                     /locus_tag="VCF75_001109"
     CDS             complement(1162..1512)
                     /gene="rbfA"
                     /locus_tag="VCF75_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEP2546675.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDKKD"
     gene            complement(1718..4579)
                     /gene="infB"
                     /locus_tag="VCF75_001110"
     CDS             complement(1718..4579)
                     /gene="infB"
                     /locus_tag="VCF75_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEP2546676.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            complement(4599..4901)
                     /locus_tag="VCF75_001111"
     CDS             complement(4599..4901)
                     /locus_tag="VCF75_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEP2546677.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAVADAGFSKKMRTLME"
     gene            complement(4894..5190)
                     /locus_tag="VCF75_001112"
     CDS             complement(4894..5190)
                     /locus_tag="VCF75_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEP2546678.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            complement(5206..6363)
                     /gene="nusA"
                     /locus_tag="VCF75_001113"
     CDS             complement(5206..6363)
                     /gene="nusA"
                     /locus_tag="VCF75_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEP2546679.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV"
     gene            complement(6538..7074)
                     /gene="rimP"
                     /locus_tag="VCF75_001114"
     CDS             complement(6538..7074)
                     /gene="rimP"
                     /locus_tag="VCF75_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEP2546680.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            complement(7182..7268)
                     /locus_tag="VCF75_001115"
     tRNA            complement(7182..7268)
                     /locus_tag="VCF75_001115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7232..7234),aa:Ser,seq:gga)
     gene            complement(7333..7968)
                     /gene="trmB"
                     /locus_tag="VCF75_001116"
     CDS             complement(7333..7968)
                     /gene="trmB"
                     /locus_tag="VCF75_001116"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEP2546681.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFFNKGQVIYRVEANF"
     gene            complement(7968..8759)
                     /locus_tag="VCF75_001117"
     CDS             complement(7968..8759)
                     /locus_tag="VCF75_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEP2546682.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            complement(8823..9857)
                     /locus_tag="VCF75_001118"
     CDS             complement(8823..9857)
                     /locus_tag="VCF75_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546683.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISIKVKERAYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNMLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            complement(9860..10585)
                     /locus_tag="VCF75_001119"
     CDS             complement(9860..10585)
                     /locus_tag="VCF75_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546684.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            10657..11076
                     /locus_tag="VCF75_001120"
     CDS             10657..11076
                     /locus_tag="VCF75_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP2546685.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQSATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEEDGISIQYTTHEPDFPVLEKLARQINQEVSS"
     gene            11073..11429
                     /locus_tag="VCF75_001121"
     CDS             11073..11429
                     /locus_tag="VCF75_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEP2546686.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISRDLAHKWRYFNQETQAHLTEIQNRMAKYQENSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(11540..12823)
                     /locus_tag="VCF75_001122"
     CDS             complement(11540..12823)
                     /locus_tag="VCF75_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP2546687.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     KLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDYTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(12832..13356)
                     /locus_tag="VCF75_001123"
     CDS             complement(12832..13356)
                     /locus_tag="VCF75_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2546688.1"
                     /translation="MVQQSLVIEEGVPQDAKAVLSLVTAAAKETDFITGVETILEATP
                     QELSGFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQAEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            complement(13331..13792)
                     /gene="tsaE"
                     /locus_tag="VCF75_001124"
     CDS             complement(13331..13792)
                     /gene="tsaE"
                     /locus_tag="VCF75_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEP2546689.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHGSAKSRY"
     gene            complement(13946..15406)
                     /locus_tag="VCF75_001125"
     CDS             complement(13946..15406)
                     /locus_tag="VCF75_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEP2546690.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSGVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDMSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIIVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
BASE COUNT         4780 a         3208 c         2622 g         4823 t
ORIGIN      
        1 atttgtaaat atataaagtt tctaatgatt ttatttctta agttgacaat tttttcattt
       61 atgatatgat tatatgataa aaaaatgata ataataagga gaactcattg aaaaagacac
      121 ttaaaagcac tcttatttta ctaggactta ctctagcaat aggagctagc actcaagttg
      181 atgccagttc tagatcttgg aaaagctggt ttatagagaa atacttttgg ttaaaaggag
      241 acaaaggcta ttattcagag caagacgaaa ctaagtttga tcaatatgtc aactctgctg
      301 gagaagcttc aaataaaggc tttgatctta aagaagaaca ggtaaatggc acattagtca
      361 ctaaaactgt tgataatatg caagttcata cttggaatga gaaccccagc aataaagcca
      421 atcaaaaggt cattttctat ttacatgggg gctcttacct aaacaaccca actacttatc
      481 atatcgacat gctaaaaacc ttatcaacta gtcttgatgc taagattatc ttaccaatct
      541 atccaaaagc acctacttac tcttacagtg atgctatgcc aaaattggtt gacctttaca
      601 gagatactct taaagaaaca actgcacaaa atatcactat tatgggtgat tcagcaggtg
      661 gtggactagc gcttggttta gcattagccc taaaaaatga aagtttaaaa caaccaaaag
      721 acattgttct cttatcacca tggttagatg taaccatgtc aaatccagat attgagagct
      781 atgaaagtaa tgatcccatt ctttcagctt ggggcctacg taaagttggg gaaatttggg
      841 cacgccgtga cccaaacaac accaaacatc catatgttag tccaattaat ggagatgtat
      901 cagggctagc acctattact atgtttgttg gtacacatga aattttctac ccagatgtta
      961 agaaatttga tgatatatta actgccaaag gtcatcaaca tgaacttatc gtcgctgata
     1021 aaatgaatca tgtttaccca atctatccaa ttgaagaagc caagactgca caatatcaaa
     1081 tcattgatac gattcaaaaa gcttactaaa aacaaaaaac ccttaaggtg actctaataa
     1141 gataccttaa aggtttttaa tttaatcttt tttatcaagc tcacgtaaca gttgatctat
     1201 tttattacca taggcgatgg aattgtcttt ttcaaacacc aaatctggaa ttttgtacat
     1261 ggtcaattgt ttgccaagtt cacgtttaat cgttccagtc gctttttcaa gccctgtttg
     1321 tgctttttga ttatcagatg ccaagtcact cataatagtg taataaactt tagcaagtga
     1381 caagtcaccc tgcatttgca cttctgtaat cgttactccc tgaacgcgtg gatcacgcac
     1441 ttttttctga agaatatcat ttacttcgcg cttgatttcc atcccaacgc ggtcaatacg
     1501 atgattagcc atagccattt cctttccaca tttttaaaac aaaagctagg ctgaaagccc
     1561 agcttatgac attgctacct gttttgataa cgacaactaa agggaatctg agaattccat
     1621 gacgagactg gaaggaatgc cccaagtagc tcatctggta gaacaagtca ttatttccga
     1681 cagaggaaaa acgtcacatc tcagaccttc ttagccccta ttttctcaca atttcttcca
     1741 taatataggc ttcaatagta tcgtcaactt taagatcgtt aaagttttca atcatcaacc
     1801 caccttcttg agcattaccg acttctttaa cgtcatcttt gtaatgtttc aagctggcca
     1861 gtttaccatc aaaaataacg acactatcac ggataacacg aacactcgaa tcacgtgtca
     1921 ccttaccgtt gattaccata aagccaccaa ttgttcccac tttagaaact ttgaaggttt
     1981 cacgaataat ggcttcacca agaatttttt cttggtaaac agggtcaagt ttccctttca
     2041 tggcttcttc tacttcttca atgactttat agataatgct atgaagacga atttccacat
     2101 catccgtatc tgcttgttga cgagcttgtg gggtagggcg aacgttgaaa ccgataataa
     2161 cagcatttga tgcttcagca agggtcacat cagactcatt gatagcacct acagcagagt
     2221 gaacgacatt gacacggaca ccttccactt caatctttac aagagatgct gcaagggctt
     2281 caacagaacc ttgaacgtct gctttgatga tgacattaac agtcttgatt tctcctgctt
     2341 taagggtatc aaagagatta tcaaggctaa cacgctgtgt attttgacgt tgtttgagga
     2401 gggcacgttt tgatcgctcc tcaccagctg cacgcgcagc tttttcatca gcgtaaacag
     2461 caaagtgatc accagccatt ggtgtttcgt tcaaaccagt aatggaaact ggtgttgatg
     2521 gctccgcaga tttaacacga cgcccaaggt catttaccat ggcacggaca cgaccaaagg
     2581 tattaccaac aacgattggg tcttgaacat gaagagttcc ttgttggaca agaagcgtag
     2641 cgatagcacc tttaccttta tctaagcgtg cttcaatcac agtaccaata gcacgaaccg
     2701 tcggatcagc tttcaattct tctacttcag caaccaaaag gactgtttct aatagttcat
     2761 caatattttt attaaattta gcagatatct ctacaaattc gcactctccg ccccaagcag
     2821 ttgagatgat accatattct gccaattctg cgataacacg ttctggatta gcacctggtt
     2881 tatcaatttt gttgatagca acaataattg gaacaccagc agcttttgag tgattgatcg
     2941 cttcaatcgt ttgtggcata acaccatcat cagctgcaac aatcaaaatg gtgatatcgg
     3001 taacggaagc tccacgtgcg cgcatgcttg taaaggccgc gtgtccggga gtatccaaga
     3061 aggtaatttt cttgccagct tcttcaatct ggtaagcacc aatatgctga gtaattcctc
     3121 cagcttctcc tgttgccaca cgtgagttac gcaaagtatc aagcaaagtt gtcttaccgt
     3181 ggtctacgtg tcccatgata gttacaacag gtgcgcgttc tacaatattt tcaggattga
     3241 ggtaattttc atcctcaaag aagcgctcga tatcagcatc atctacttct accttagcct
     3301 tagcctcaat accataatct accatcaata attcgatagt atccccatct aaagactggt
     3361 tttgagttgc cataacaccc atcataaaca gctttttaac aatttcagcg ggttcgcgtt
     3421 tgatacgttt tgcgatttcg gcaactgtca tgccttcagt atattcaaat tcttttggca
     3481 attcatggaa tttacgctcg gttactggtt ttggagcggt gttagtattc ttaacatttt
     3541 tgcctttttt ggtcttttta tttttattcc agttactatt tttttgattt ctcacttggt
     3601 tttgactatt ccaactcttc ttatttttct tttgtccttc ttgtgaatga tgagttaact
     3661 cacgattctt ttcaggacgt gcttgttttt tacggcgatt atcagcagta gatttagcac
     3721 taatatttga tgactttaat ttagtttctg cttcttcaat agatttcagt gctgcttcac
     3781 gatggctcat ctcctcagct tgtgctttta aagccgcttc tttagcttct tgtcttgcca
     3841 actcagctaa acgcttagct tcttgctgtg cacgaaaacg tgtttcactt tggcgtgagt
     3901 attcagcatt ttgttcagct tttaaggcag cagcacgagc tttgaaatca attttaggac
     3961 ttttcgattg aggctcttgt cgtccttgct ttttctggtt tgtgaatcga ccattcttgt
     4021 gattctcctt agcaaaggaa cggttacgat caccaactag ctgatgatta tcatgtctag
     4081 gtgatacatt cgatgctttt cgctcaatac gcttatcgtt tgtatcacct tgagaacgat
     4141 gggtaatcct cttatctcct tgttcgttag tttgatgatt attgggctgg cgataacctc
     4201 ctcgacgttc ttgagaacga tgagaagagc catttgcttg ttttcgagca gcctgttcct
     4261 ttgcacgagc ctctctttca gctttaaaat tacgactctt tggcttagag acctgtgttt
     4321 tctctacatg ctcagaacct gtttctttga ccactgttac agagggttga gcaacttctt
     4381 tgggcttgac tgtttgagaa gctgtcacat caggtttaga agcctttgaa aaacttgaaa
     4441 taatcttttt cgcatccgct tcttcaacac ttgatgcatg gcttttcaca tctaatccta
     4501 agtatttagc gtgttcaacc acctctttac tacttttacc aatttccttg gcaatttcat
     4561 gtaatctttt ctttgacaat ttgtgtcctc ctattcatct attccataag agtcctcatt
     4621 ttctttgaaa atccagcatc tgcaacggca gcaaccttcc ttggtttacc tagtgcagca
     4681 cttaattcca gtgcattcaa cactgtggag acttctacat tataataatt acttttatct
     4741 gtcacttttt ttgtcacatt aggaccagca tcattggcta aaaatactaa tattacctgc
     4801 tggtgttgaa tagcttttac tactagttct tcacctgaaa tcacttttcc agcccgttga
     4861 gctagaccaa taagactaga taatctttct aaattagtca agacctaact ctcttctttt
     4921 gattttgtga tcaacataag caatcaagtc atcgtaaaaa ctttctggaa tgtccattga
     4981 gaaactcctg ttgaaaactt gttttttctt ggccattaag gcctcttgat tgtctaattt
     5041 gatataagca ccacgaccat tttgtttacc agttggatca atgaaaactt gaccatcctt
     5101 agtttttaca atcctaagaa gatctcgttt agcaataatt tcaccagaaa caagtgattt
     5161 tcgtaaaggt atttttttga ctttagacat caaacacctc ttttcttaca cttcctcggc
     5221 agtcataaca tcgtcaacaa ctggctcttc cacaactgtt gctgcttctt tttccgcctc
     5281 aagacgatca tattcgctag ctgatttgat atcaatacgg tagccagtca aatgtgccgc
     5341 taaacgaacg ttttggccgc gacgcccgat agctaatgat aatttgctat caggaactac
     5401 taccgtagca cgtttaagat cttcatcgtc aaaaagtacc atgtctactt cagccggagc
     5461 aatggcattg tagatgaact cagctggatc atctacccat tgaatcacgt caatattttc
     5521 ttcaactgga atttcaagcc ccgtcttagc atctacacgt tttggatgga acttgctgat
     5581 gaccttttta atattacttc caccacgtcc aacaattgtt ccgatggcat caacgttagg
     5641 attatgactg cgcacagcaa ccttggtacg gtcacctgct tcacgagaga cactcatgat
     5701 ttcaacagta ccatcaaaga cttcaggaat ttcttgctcc ataatacgtt tgatgaattc
     5761 tggatggcta cgactaacaa agacgttaac tcctttagga ttattttcaa ctttgtagac
     5821 ataaacgtca atacggtcat gtgatttgaa agtttcacct ggaatctggt cttggtgaga
     5881 caattgtgct tctaaggaac caagattgac ataaataaaa cgttgatcaa accgttcaac
     5941 tgttcctgtc ataatttcac cttcatgctc tttgtattca ttgaacatca cttcacgcat
     6001 ttggcggcgc atcttttcca taatagtctg cttagctgat tgagctgcga cacgtccaaa
     6061 ttcattgaca gattcctcaa agcgaatctt atcacccaat tcataggcag aactgatagc
     6121 aagagcatct ttcaaactaa tctcaagccg gctgtcaaaa acttcttcaa caacctcacg
     6181 aaccgtgaat acttgaaaat cagctgtttt ttcattaaat tcaataacac atgattctga
     6241 ttgaccatag cgacgtttat aggcagactt taatgattct gtcactgcgt caataatatc
     6301 tgctttatca atgtgttttt cttcttccaa aatacggaag gcttctagca tttctttgct
     6361 cataatgtta tctaccttgc ttgtaaagct tggcaaaaat cctttcttaa attaatacga
     6421 tcaatagccc cctaaaggct cccttaattc cttttgttta gcttgtataa actctatagg
     6481 tgttgtttca aatttgctta agcgttccta aaaaagttta gtgctttgaa ataaaagtta
     6541 taatttgaca gccattctca ccttagcaac tgcttgatat ggtatattca cgatcttatg
     6601 acgtgtttta tcaagataat caatggtcaa cgtttcacca tcaaaggcta ataagtctcc
     6661 ttgaaaaact ttcaccttat caatagcttg ataaagactc acattgatat aagagccaac
     6721 tgctgctttt aaactgtcag ctgtcttgag cggacgctcc aaacctggac tagagacttc
     6781 tagcatatat tggtttggaa aaggatctgg atcaatcgta tctaacagcg gactaataat
     6841 attcgtcaag tcagatgtat cttccactgt gattccacct tccttatcga ctaaaatact
     6901 aaggatgtag tcactaccca ttttgtcata ctcaacgtct actaattcat aaggtgcctt
     6961 aatctcaggt gttactgtct tggttacgat atcgataata gatgtatttg ctattttgat
     7021 tacctcctct atttttatgc tcttctctaa gataattggt ccttgggaat ccacttaata
     7081 caaaagtggt gagacatatc ccaccacctt tctgtattat ttaagcctat tataccataa
     7141 tttaagagga atgcaaggat atgcactttg ttttactcac aaggagagga ggggattcga
     7201 acccccgagc cccgttaagg actacacgct ttccaagcgt gcgcactaag ccactatgcg
     7261 acctctccat tactaaagaa gttaatactg catcaactcc tttatgttat catattttga
     7321 aacaaaatac tactagaaat ttgcctcaac tcggtaaatg acttgacctt tattaaagaa
     7381 cttctcttcg tattctgtca tgacatttcc ttcgtagtta ctagcgtgta agtcaagcca
     7441 gatctgtctc aaggtcatgc cgtattgaga aaaactagct aaactgtact caaacaagcc
     7501 ccgattatcg gttttaaaat gaatttcacc gtgttctggc aagatacgct tgtaagtgtc
     7561 taagaaatcc ttgtaggtca aacgacgttt ttcatgcttg gtttttggcc aaggatctga
     7621 aaagttgaga tacatcatat ccacctcacc atcttcaaaa tagttggtta aacttgatcc
     7681 atccactcgc agaagtttga cattgggtac ttctgaagct aataccttat ccaaggcgta
     7741 gcttaggaca gataattgaa tatcaatgcc aatgtagtta atatcaggat ttttcaaggc
     7801 catccctgtg ataaatccgc cctttcctga accaacttcg atatgaatgg gacggtcatt
     7861 tccaaaaacg tcatgccaac ggcccttggc atcttctgga tttaatatca catagtgtgg
     7921 gttattagct aagtgctcct cagcaccttt tcgttttcta actcgcatta tttctttcta
     7981 aatatttctc taaattttct gagggcataa atctcctgat tcacatgctc catgtcacgc
     8041 ttgtcaaaac acttgagaat ttgtgtcagg tgagaaaatt gaccatacca aataattttt
     8101 tgcataacct tgtcattatt tttatagcca taataagaca gccattctga ccaacgagac
     8161 cgtggaatat agtggctcaa caggtaagca acatcataca tccgatcagt cagacgaaca
     8221 gaatcccaat ctactaaaaa aatcatacca ctagtagtaa tcacccaatt gctatgttta
     8281 atatctccat gcacaatcgt tgctacttct gatttgaact ctggtaagct ccgttttaat
     8341 tctttaacga tagcttgtaa gtatgaattt tgctgaattt gcaagggtgc attttgctca
     8401 aaatcaacca ataaatcgta tgggttttca atcttataat tgagctgaag cagttgattg
     8461 actaattttt tagatttgtg aaggcgcaat agaatatgaa tgatttgctt actgttcata
     8521 tcttctttgg tcaatgtacg gccgttaagc cattcttggg cactcatcat atcaccattg
     8581 cccatgcgtt tggcccaaag taactgtggc gcaatctgtt cttttgctaa ggcaggtaga
     8641 ataggggtcg tatttaattt tataaagaca cattccccat ttggataagt gcctttataa
     8701 gctttgccac ttttcccacg taagggagtc aaggtaagtt cttgttccgt cgttgtcacc
     8761 ttatcgccct ccttttctat cttttatagt cgtttacccc tattttactt gttttaggct
     8821 acttagtcaa tgatcttctt taacttataa ggtagatata tacaagctat gaaaaccata
     8881 cctacaatca aaatgagtgc tttgggcgct gatgagcaac acagcatatt gacgattaac
     8941 attaaaaagc ttaaacctcg taaaaaactt aacatatttt tcttcttgct ccgactattt
     9001 tcaggataaa gactagtcat ataatggtaa tcgtagtgat aataaagtga aagtaattgg
     9061 aatacaacta aatagttaaa aatgagtgct aaacttactg acaaataaag attatggata
     9121 aaaaatacag ataatgaact taatagcatt agtcgtaaaa ataagcccaa ataatctgaa
     9181 ctacgtaaaa aggctctagc ataaagggac agccaaaggt ttgaaggagt ttgcttaact
     9241 aattttaaaa gaggatttag ataagctctt tctttcactt ttatagaaat tcctttgact
     9301 gttgtaaaaa gagaataaaa ctttaaaata ctttgcttgc gattgctttc aaatgcgaca
     9361 gcttttgccc aatctaaacg atttcctctc aaaaaatatc ttgtctttcg actaagcact
     9421 actcttttta gtgccaataa tatcaaaact aagataataa aaataaataa agacagacct
     9481 agacgtctaa aaataggata taaaaagagc aatacagcag tctgcaaagt tccccaaagc
     9541 aaaaaagaga gtcgctcagc ttgtttgata taagaaatca cttcttcttc ttttggaagt
     9601 aagaaatgct gatctgcctc ttctagatac gttgctatag atcccatact gagcaataat
     9661 gggatcaata tcccaagaca aacctgaata ggtagatgat tcgttggaaa atggtttagt
     9721 agctgaccat actgcaccat agcaaagcct aataaaaaca tcaacaccaa aacaaagtga
     9781 tcatttaaga cgtaacgaag gtatttgttt tgttgttttt gaaagtcttg acggcgtttc
     9841 agaaataatg ctttcatact taatcctctt ttgttaatgc aagatagata tcatttaagc
     9901 tcgctgaacg gtcaccaaaa gcttcttgca agtcagcaag agttccttgg gcacgaactt
     9961 gtccatgatg caaaatcaca aagcgatcac acattctttc agcagagtcc aaaacatgtg
    10021 tactcatcaa aatagatttt ccttttgctt tttcgacttc tagcgtttgg attaagtctg
    10081 aaatagctaa agggtctaac cctaagaatg gttcatcaag aataaataga cttggatcaa
    10141 tgacaaaggc acaaataatc atgacttttt gtttcatccc tttagaaaaa ttgacaggaa
    10201 accattcaag tttgtcagtt aaacgaaaca tttccaagaa aggttgtgct cgtttttgtg
    10261 ccacttctaa gtcgatatca taagccatag ctacggtgtt gatatgctct gataaggtca
    10321 attcttcata gagactagga gtttctggga taaaacctat tttttgacgg taggcaactg
    10381 cattttctgc tagagttaaa ccatcaatag aaatacttcc ttgataaggc ttcaaaaagc
    10441 caataatttc gttgatagtt gttgactttc cagcaccatt tagcccgatc aaaccgacta
    10501 actctccatt gtctacagaa aatgagacat cgtttaaaac agggatattg tgatatcctc
    10561 ctgttagatt tttaatgttt aacatataat ttctttctag tttttgatat acttataata
    10621 tattataaca aattcaagta gagaaggtga tataaaatgg aaaattgtat tttttgcagc
    10681 atcattcaag gggatatccc ctcttcgaaa gtctacgaag atgagcaggt cctcgccttt
    10741 ttagatatct cacaaactac aaaagggcat actctagtca ttcctaagca acatgttcgt
    10801 aacttactag agatgactgc tgagacagct agtcatctct ttgctcgtat tcctaaaatt
    10861 gctcgtgcta tccagtcagc tactggcgct actgccatga atatcattaa caataacgaa
    10921 gccttagcgg gacaaactgt tttccatgct catgttcatc tcgttcctcg ttataatgaa
    10981 gaagacggta tctcaattca atacaccact catgagcctg actttcctgt cttggaaaag
    11041 ctcgcccgac aaatcaatca ggaggttagt tcatgaaagt taaaaccgtg atagccgtcg
    11101 gattattatc ttttaccgct tacaaagcat atcaaaaacg ttgtaccata aaagagctgt
    11161 tatccatcag tagacaagca aaagatgctg cccaacttga tttagataac attaaagcaa
    11221 accttgacct cattcacagt caaggaaaag taattcaaaa tattagtcgg gacttggccc
    11281 ataaatggcg ctactttaac caagagacac aagcacacct cactgaaatc caaaatcgta
    11341 tggccaaata tcaagaaaat agtgagctaa tttctagtga atcacctact tgccaaaccg
    11401 tcattttaac taacaaccat tccagatagt attatctccc tcatacactt tatttgaaat
    11461 cacagacagc tatcatcttt gatacctgtc ttttttatat tcaaaatcgg atattttaaa
    11521 cataaaaacc ctcagaacgt taggtctgag ggttgatatt agcaggagga actaatgatc
    11581 cagaccctga tgagctagac tcatcagctg ctaaagcatc cgatgaagaa gacggcgtag
    11641 ttggcgtatg gcttgaagca gtatagtcct ggtctgtact agcactctga taagatgagc
    11701 cggtatctac tccacttgaa taattagcgt aagagctatg actatcagaa taagatggag
    11761 cctggcctga agagtcatag ttgttgttta ctgtctgaga cttagttgac ccatacaact
    11821 tttcataaac agtagcattt gtttttaatt gactaacctt atgaagtcct aattctgttc
    11881 ggatacgatt ttggatttct aacaaatgat tagaggtaac aatttggtat gatccaccat
    11941 ctgataaagt ggcatcttct ccttttagtt gataagtctt aatagttcta agtgcgtcac
    12001 gataacctaa tagactaggg atagtgcgag aagagatttc gatattcgtt tgcatattac
    12061 tacttacagc agataaaatc ttccgataag agctaatgct atcaagagca aggatttttt
    12121 tcaatacctt ttgaatgact tcacgttgac gcttttgtcg accataatct ccctcaggat
    12181 catcataacg catacgagca taaaccaaag cttgttcacc gttaattttg tgtgttccag
    12241 gcgcaacagt agcttgatat tcaggttcgt tttcagcaat cgagatagga aaatcaaact
    12301 catttgtaac tgtaatccct ccaactgcat tcacaagatc aataaggcct tgcatattaa
    12361 tttgaacata gttatcaatg gtgatattca aaagatcttg cacggtcata atagccatct
    12421 gagcgccacc tgctgcataa gcagcgttaa gcttagcttc aacaccattc atttcattat
    12481 ttttgggtcc agataacgtg gttaaggtat ctcgttctaa actagtcata gttgttttct
    12541 tggtctttgg attaaccgta accaaaatca tcgaatcact gtttccttcc cacttggagg
    12601 cacgctctga agagcctgta tcaacaccca tcaacaagat agaaaaagct cttgtttgtt
    12661 taatggcatc acttttcttt gaagatgtcg aaaaatcttt aaaggtcttt gataattctc
    12721 ctgttgagaa ggtataagca ctagttagat agacacctaa tgccaagaca gttgttaaca
    12781 caatagctgt gaacattaaa actatttttt ttccaatttt catcgattca cttactgact
    12841 tacagattta cgcatatgat aaacatctaa gtcatctcca ttttttgatt tgatgtcgtt
    12901 ttttcgcata ctttcaatac gaaaaccata ctttttatac aggtaaatag ccttagtatt
    12961 tcttacttga acgtccaact taaggctttc gatataaggg ttttcttcag cccaatcaag
    13021 agcaatctct aacaataatt gaccgatacc ataaccacgg taagtcttag caactaacat
    13081 aaatacatct gcttcagctt ggccttggga taatacttcc ccagaaagat ttaaaagccc
    13141 tactactttt tcatctaatc tagctagaag gcaaaaatca ataaaagagg tttgacttct
    13201 tgataaaaag cctgacaatt cttgaggagt agcctctaat atagtctcta cgcctgttat
    13261 aaaatctgtt tctttggcag ctgccgtcac taaggaaagt acagcttttg catcttgagg
    13321 tacgccttct tcaataacga gactttgctg aaccatgtga cattatctcc agaagctgac
    13381 gagagcgctc accatgcgcc aacaagtcta gttgacgacc tttgtctcgt ttagtaatgg
    13441 taatttgcaa atagtcttgt aataatccct ctcctaataa ttctccccac tcaataacgg
    13501 taacaccatt accaaaaaga aaatcatcta aatcaataga gtctggatca tcaccaacac
    13561 ggtaaatatc taagtgataa agaggtagac gtccttcata ttctctaaca atagtataag
    13621 ttgggctttt gatcatttga ctaatcccca tccctttagc aatacctttt gccaatgttg
    13681 ttttaccggc acctaagtcg ccagacaata caatcacatc tccaatagac aaataagtac
    13741 ctagggtttc tccgtaagct ttaagagtat attcattttc actataaaac atagcaacat
    13801 tatatcagaa aaaaaacata cttccaagca ctacctagaa gatgactcca ataaggtatt
    13861 cgtaaagtaa aaacgcttct ttatattaaa ggttaaaatc aattaaagaa gcggcagtta
    13921 gtagcaaaat aagtaaatta tttggttata agatagttaa tatgatgaaa tttacaatga
    13981 acaagaaggt tgctccccaa ataatagggt gaatatcttt tgtttttccc tcaacaactt
    14041 ttactagaca atagaaaata aaggcagcgg caataccata ggaaatagag taacacagcg
    14101 ccataaagaa agctgcaaaa aaagctggaa gagcatctgc aaatttactc caattaacat
    14161 caagaaaaga agacaccatc atgacaccca caataattaa agctggagcc gtagcagcag
    14221 ctgggacaat acctacaagc ggtaatagca atattgataa taagaagcat actgcggtgg
    14281 agactgctgt tagtccagta cgtccacctt cagcaattcc tgctgctgat tcaacatagg
    14341 tagtcgtatt tgaagttcca accaaagccc caatagaagt accgatagcg tctgcaaaaa
    14401 gcgcacgatc catttttgaa ctaaagccta tactattttc caaagcattc tcatcgtctt
    14461 gagagaaaat acctgttcga cgaccagttc cgataaaggt accaattgtg tcaaaagtat
    14521 ctgatagact aaaagcaaaa atagtcatga aaactagcgg taaacgactt gaatcgctaa
    14581 aaagagaact caaaccatcg aaagctgcaa ggaaagttgt acctaattca gtccaagctt
    14641 caccaatatg gctgatccca aaattgaggt tggacatatc cactattcct aaaggaatac
    14701 ctacaagagt tgttgcgaca ataccaatta aaatagcacc tctaaaattt cgaatcacaa
    14761 gagctgtcgt taaaagtaaa ccaaaaatag caagtaatac accggaatcc gtaaaactag
    14821 aaatcgtagg tacaactcca ccattggcgt ctacaaataa tagaccatct gcaaatgttt
    14881 tagccgatgc tttagccggt tcaacaccat ttaccattac tatattttca gcagagatag
    14941 aaaaagtaat gatatttgcg tttttaaatc ctaaataagc tacaaagaca ccaattcccc
    15001 caccaatagc atgctgtaaa ctaactggaa tcgccttgat gatactttta cgaaacttgg
    15061 ttacggtaat aaaaatattg aataatccac aaatgaaaac cattgccaag gcttcttgcc
    15121 atgaaaaccc taaagcaaaa acaactgtat aagtgaaaaa agcgttaagt cccattcctg
    15181 gtgccaacgc ataaggcaca ttggcaaata gtcccataat taaggttgat atggctgctg
    15241 cgataatcgt agccaaaaag acagcattag agggcatccc cgctgcacct aaaatacttg
    15301 ggttaacaaa caaaatatag gacatggcaa aaaaggtcgt taaaccagcc ataatctcag
    15361 ttgagacagt tgtcccattt tcgcttaact taaaaaactt ttccattgaa atggagtctc
    15421 cttaattaaa tag
//
LOCUS       DATURV010000037        15362 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DATURV010000037 DATURV010000000
VERSION     DATURV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15362
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.037"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            157..840
                     /locus_tag="VCF75_001126"
     CDS             157..840
                     /locus_tag="VCF75_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEP2546691.1"
                     /translation="MEWIKLIGIVIIVLGFILKCDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYGGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            840..1766
                     /locus_tag="VCF75_001127"
     CDS             840..1766
                     /locus_tag="VCF75_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEP2546692.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            1816..2463
                     /gene="pcp"
                     /locus_tag="VCF75_001128"
     CDS             1816..2463
                     /gene="pcp"
                     /locus_tag="VCF75_001128"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEP2546693.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERIAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(2581..3300)
                     /locus_tag="VCF75_001129"
     CDS             complement(2581..3300)
                     /locus_tag="VCF75_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEP2546694.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(3306..3773)
                     /gene="smpB"
                     /locus_tag="VCF75_001130"
     CDS             complement(3306..3773)
                     /gene="smpB"
                     /locus_tag="VCF75_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEP2546695.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQESDIKKQMKHYNAR"
     gene            complement(3776..6109)
                     /gene="rnr"
                     /locus_tag="VCF75_001131"
     CDS             complement(3776..6109)
                     /gene="rnr"
                     /locus_tag="VCF75_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEP2546696.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNGVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINPQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKVEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYNQPSQEKRDHFAQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGIK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(6203..6439)
                     /gene="secG"
                     /locus_tag="VCF75_001132"
     CDS             complement(6203..6439)
                     /gene="secG"
                     /locus_tag="VCF75_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEP2546697.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(6485..6631)
                     /gene="rpmG"
                     /locus_tag="VCF75_001133"
     CDS             complement(6485..6631)
                     /gene="rpmG"
                     /locus_tag="VCF75_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP2546698.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(6628..7821)
                     /locus_tag="VCF75_001134"
     CDS             complement(6628..7821)
                     /locus_tag="VCF75_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEP2546699.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVAPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(7943..9445)
                     /locus_tag="VCF75_001135"
     CDS             complement(7943..9445)
                     /locus_tag="VCF75_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEP2546700.1"
                     /translation="MTTMTIAKGNQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQSPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSEI
                     ESQQLVQSSPFASKYSQSIDRESAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKA
                     QAATKKANRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(9635..10228)
                     /locus_tag="VCF75_001136"
     CDS             complement(9635..10228)
                     /locus_tag="VCF75_001136"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEP2546701.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNNGDMETLIKQVQSALLSLANPR"
     gene            complement(10225..11052)
                     /gene="mutM"
                     /locus_tag="VCF75_001137"
     CDS             complement(10225..11052)
                     /gene="mutM"
                     /locus_tag="VCF75_001137"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEP2546702.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLITKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(11224..12090)
                     /gene="rgg2"
                     /locus_tag="VCF75_001138"
     CDS             complement(11224..12090)
                     /gene="rgg2"
                     /locus_tag="VCF75_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEP2546703.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            12179..12247
                     /gene="shp2"
                     /locus_tag="VCF75_001139"
     CDS             12179..12247
                     /gene="shp2"
                     /locus_tag="VCF75_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEP2546704.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            13161..13430
                     /gene="stcA"
                     /locus_tag="VCF75_001140"
     CDS             13161..13430
                     /gene="stcA"
                     /locus_tag="VCF75_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEP2546705.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(13778..14176)
                     /locus_tag="VCF75_001141"
     CDS             complement(13778..14176)
                     /locus_tag="VCF75_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEP2546706.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDTAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLTHHLLHSDGVLPSLNDKSA"
     gene            complement(14269..15342)
                     /locus_tag="VCF75_001142"
     CDS             complement(14269..15342)
                     /locus_tag="VCF75_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546707.1"
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFFSDQPSQVVLYRGEANTVRFTYTDQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSAREIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGALPDNIK
                     VSINPKGGGLATITYGAGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRD
                     QSLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEV
                     GSWLEPATALPSVEMSAEDRLKS"
BASE COUNT         4491 a         3411 c         2750 g         4710 t
ORIGIN      
        1 ctttttaagc ccgttatacc aaatgataat gacctttcca aaatagtatc accttcctta
       61 acactttaag tagaaaagga actattaggt tgagcctttt tgcaaatcct agcgactaat
      121 gttataatcg tcatactatt gaaaaaggag ttctttatgg agtggattaa attaatcggt
      181 attgtgatta tcgtattagg gtttatttta aaatgtgatg ctatcgctac cgtagttgtt
      241 gcaggacttg ttaccgcgct ggtttcagga atttcgttta ttgattttct agatatttta
      301 ggaaaagaat ttaccaatca acgtttgcta accattttct tcatcacttt accgcttatc
      361 ggcttgtcag aaacttatgg actgaaacac cgagctactc agttgattca acgtgttcaa
      421 gcccttaccg tcggccgttt attgactctc taccttatta ttcgagaatt agcaggtctc
      481 ttttcgattc gactaggggg acatccacag ttcgttcgcc ccttgattca acctatggga
      541 gaagctgctg ctaaagccaa cattggagaa gagttgaccg atgcagaaaa agatgacatc
      601 aaagctatgg cagctgccaa tgaaaacttt ggcaatttct ttgcccaaaa tacctttgtt
      661 ggcgcaggag gtgtcctact tatcgctgga actttagaac agcttggcta tggtggtaac
      721 caagctaaaa ttgcattttc ttctatttta attgctatta tctctattat tatagttgct
      781 atttacaatt acctctttga gaaaaaaatg gaacgtcaac atcagaaagg agataactaa
      841 tggctaacat tgcaaacctt gttttagaaa cgatatatat ccttatcggc ttgcaactgt
      901 ttcacacagc ctactgcacc tttaaagata aaaccaaccc tgtctatttt gggacagctc
      961 tgttctgggg attgctgggt gttaccttcg ttggcggtgc ctttttgcct aacaaagtta
     1021 tcggttttat cgtgattgtc ctagctcttt taaccttgtt taagcaagtt cgtattggaa
     1081 cattaccagc ctttaatgaa caaaaagctg aagaaagtgc tcaccgcatt ggtaactgga
     1141 ttttcttacc agttatgtta atggctatga tttctctact tcttgccttg atccttcctg
     1201 atttcagtaa aagtgccatt gggattgctg gtatcttagc cactattgct attttaataa
     1261 tcactaagca aaaaccatct gctcttttag ctgaaaataa tcggatgaac caacaagtgt
     1321 ccacaagcgg tattttgcca cagctcttag gagctttagg agctattttt gcagctgctg
     1381 gtgttggtga tgtgattgct tctctcattc gggagattgt acctgctgat agccgcttct
     1441 ttggtgttct cgcttatgtg cttggcatgg ttatttttac tatgattatg ggaaacgctt
     1501 ttgctgcctt taccgttatt acaaccggta tcggtgttcc ttttgttttt gctttaggag
     1561 ctgaccctat tattgccggt gctttagcta tgactgctgg tttttgtggt accttattaa
     1621 caccaatggc agcaaacttt aatgccttgc ctgttgcttt gatggagatc aaagatcgaa
     1681 atgcagttat caaaaagcaa gctcctatcg cccttgtttt aattattagt cacattgcct
     1741 taatgtattt acttgcttat tattaacttt tttgctaagt tagtagcgtc agttattcat
     1801 tgaaaggaca ttattatgaa aattcttgta acaggctttg atccctttgg cggcgaagct
     1861 attaatcctg cccttgaagc tatcaagaaa ttgccagcaa ccattcatgg agcagaaatc
     1921 aaatgtattg aagttccaac agtttttcaa aaatctgccg atgtgctcca gcagcatatc
     1981 gaaagctttc aacctgatgc agtcctttgt attgggcaag ctggtggccg gactggacta
     2041 acgccagaac gcattgccat taatcaagac gatgctcgca ttcctgataa cgaagggaat
     2101 cagcctattg atacacctat tcgtgcagat ggtaaagcag cttatttttc aaccttgcca
     2161 atcaaagcga tggttgctgc cattcatcag gctgggcttc ctgcttctgt ttctaataca
     2221 gctggtacct ttgtttgcaa tcatttgatg tatcaagccc tttacttagt ggataaatat
     2281 tgtccaaatg ccaaagctgg gtttatgcat attcccttta tgatggaaca ggttgttgat
     2341 aaacctaata cagctgccat gaacctcgat gatattacaa gaggaattga ggctgctatt
     2401 tttgccattg tcgatttcaa agatcgttcc gatttaaaac gtgtaggggg cgctactcac
     2461 tgactgtgac gctaataaac ctattttaaa aaaaacagag atatgaacta actctgtttt
     2521 ttttgtgcta aaaatgaaag acctagggaa acttttcata ggtctttctc aattgtcatc
     2581 ttaatctaat actacttcta acatcagcgg gtatagtttg ccagtaatta agaaacgttg
     2641 ttgatctaaa tgagcaatcc cattcaaaac attaaggtca gggtaatggg acttatcaag
     2701 atttaaggct tttaacaaag gactaatatc ataggtggct accacctttc cagaatcagg
     2761 ttggagtttg acaatagtat tggtttgcca aatattggca tagagataac catctacata
     2821 ctctaattcg ttaagcattg agatggggac actttctata gcaactagta cggtatccaa
     2881 taaagcaaaa tctttggggt ctcgcttttg caaaaaagca tttccactag tcatccaaag
     2941 acactggttt tccttatcat aggctaatcc ccagccatct ccttcaaatg gataggcccc
     3001 taaacaattg caggtagcct tatcaaactt gtaggctaca ccctctttat aggttaataa
     3061 ccaaaaataa tcctcaacaa ctgttagccc ttctgcaaaa actgtatctg gaaaagctat
     3121 tttttctgaa aagatctctt gagtaaggtc atacacccct acttttgaga aaccataacg
     3181 accggcacta agtaatatat gattgttgtt gagttgttct aatccctgcg tgtataagtt
     3241 gctatcataa gaataagttc gtaacaaacg aacttgtcct ttagttatca taatactcat
     3301 tcaccttacc ttgcattgta gtgtttcatt tgttttttaa tatcactttc ctgatcacgg
     3361 cgtttaatgg tttcgcgttt gtcatactcg tgtttccctt tagctagacc aatcagaact
     3421 ttagcaaagc catccttgag gtaaactttg agaggaacca aagtcatgcc agatccttta
     3481 agttcatttg ctagatgcgt aatctcacgt tttttgagca gcagtttgcg ggttcgttca
     3541 ggatcagcgt tccaaatatt accttgttca aaaggtgcaa tatgcacgtt gaccaaccac
     3601 gcttccccat tcttaatttg ggcaaagcca tctttcagtt gaatacgggc tgcacgaaca
     3661 cttttaatct cagttcccgt taagacaatc cctgcttcaa cggtttcaac aatgtgataa
     3721 tcatgtctag cctttttatt ttgcgccagt atatgacctt ccccttttgc catagttaac
     3781 tccttttacg actcttcttt tttgcttgtt ctttgtaaaa tggtttttta gacccttttt
     3841 tagttttacc cttggttttt gccttggcta cttctttagg ttcttttttc tttatccctt
     3901 tagatgattt tgaggattta cggcgatctc gtcgactagc tttatccgac atcttgattt
     3961 tttcaaccac atcaaaatcg ctaggtaaat actcaaagtc aatgtcacca gtttccttgt
     4021 cagccttaac caatttcaca cgaataggtt gaccaacttt aaagacttta cctgattttt
     4081 caccttgaag gcttagcgtt ctctcgttaa agtgatagta ttccggtaag ctagtgatat
     4141 gaaccaaacc ttcaatcgta tttggcaatt ctacaaaaaa accaaattta acgacactgg
     4201 ccacgatacc gtcaaattct tcgccgacat actctgccat atactcagct tttttcatag
     4261 cttcaaccac gcgttcagca tcaatagcac gacgctctaa ctgtgaagaa gatgttgcca
     4321 gctctggaat gatctgagca aaatggtcgc gcttttcttg actaggttga ttgtactcac
     4381 gaaccatgcg gtgaaccaac aagtcagggt aacgacgaat cggactggta aagtgagtgt
     4441 aatattctgc ggccaaaccg taatgcccat gattatgttc agaatatctt gcttgttgca
     4501 tggaacgtag aagcatcatg ttgagcactt ctgctcctgg ttgaccttcg acttttgcca
     4561 taaaggcttg caaggcttcc tgagaaatct tgttggctgt tccttgaatg tgaataccaa
     4621 aggtgctagc gtagtcaata aattgttgaa gtttttcaac ctttggttct tcgtgaatac
     4681 ggtagataaa cggtaactta gcttttgcaa aatgttctgc cacacactca ttggctgcta
     4741 gcataaagga ttcaatcatc cgctctgcaa ttcccctttg acgaagcacc acatcaaccg
     4801 gcattccttt gtcattaaca ataactttag cttcttgcgt atcaaaattc aaggcgcctc
     4861 gttttgaccg catagcttcc aaaatcctat gcaaagccac catgagggtc acatcatcag
     4921 caatgctagc gaattcttgg agcgcttctt catctcctgc aatcatgtca ttgaccgtac
     4981 tatacgtcat tctgtaagtt gtcttaatca ctgattgaca gatttgataa ttgaccacat
     5041 gcccctgcgg gttaatttcc attaaggctg actgggtcaa acgatcaacg ttaggattca
     5101 aagagcagat gccatttgac aaacgctctg gtaacatcgg aaccacacga tcggtgacat
     5161 agacagaagt gccacgggcg atagcttcct tatcaagggc agatccttct gtcacataat
     5221 atgagacatc cgcaatatgt accccaagtt catagttacc gttatccaat agcttaatat
     5281 ggatagcatc atctaagtct ttggcgtccg caccatcaat agtaatggtc gtttcttgac
     5341 gcaaatccac acggcctatt aaatctttag cagttggtgc ttctgaaata gcattggcct
     5401 cggctaaaac ttctgctggg aactctgaca cgatatccat cgattcaagg acttctagga
     5461 catcaattcc aacgtctcct tgatggccta cgatatcacg cacactggct acaaaataat
     5521 cgtgcccgcg tgtcgggtat ttatcaatat caaccttgat gatttcagtg cctttaagca
     5581 caacaggttc ttttttgata tagatttttt gttgaatctt ttggtttttg gacctgatat
     5641 aaccagcata ttttggtttg tcatcatcta aaataaaggt tccaacagct gtttttaaag
     5701 aacggtcaac aatagcaacg accttagctt cagccgctgt gcctttgaga cggtctgctg
     5761 gctttttaac cactacttcg acagtatcac catcaatagc atacccaaca ccattacgac
     5821 cgataaacat atcgtcttca ttctcatcca catgtaagaa accaaagcct gctttattgg
     5881 cacgaaaaac accctgaaca gtaggttctt ttttcttttc acgctctttt ctaagagcca
     5941 gactgccatc atctgaaaac cgtaacaggc tctgactttc cattttggaa atggttttaa
     6001 taaggctggg aaaatgctta gctccagcca tctccaaggc cgcagccaag tcgttcacat
     6061 tggacttgcc atgctccttg aggtaagtca taattctttc tttcatcata cctaatttct
     6121 atttctgttt tgtttgttgg aggatatcaa tccgtttcgc tgataaaaaa atgagctaga
     6181 ctaactctgt caagctcact ccttatttac ttgataaaat ggcaagcaca agtgcaatcg
     6241 ctagccaaaa aaatactaga actgctgtga atcgttgcat gaatgcttca aagccacgag
     6301 cttttgtacg ttcaaacaag gcttctgaac cgctgctatc aaaaacattg ctacttgggt
     6361 ttttttgagg ttgcataaaa atagcaatca caagcaatac cgacagtact agcaatatcg
     6421 taagtagtaa gttgtacatg ttatttcctc cactattgtc atgaccattc taacacaatt
     6481 cttatcaagt ttcaacatgc agggttactt ttctctcctt ggggcagtat ttgggaactt
     6541 ttattttttc cggatgactg cttttatttt tactggttag ataattgtta ctgccacact
     6601 cactgcattc taaattaatt tttactctca cacaatatcc ttaactttaa tatattttcg
     6661 aaatataatc aaactccata ttaaattaac aaacacaatc aaactagtca cataaaaaac
     6721 agaacggtag cctagcacta cagccacatt tgatccgata aaagggccaa tgacttgacc
     6781 aaggttacta aacatttgat tataggcaaa tactctagaa ataccttctt ttggtgttaa
     6841 cttagttagt aaagagttaa tacttggcat caaagcgccc acaccaaacc cataagcaaa
     6901 gcgtagtact cctaattgaa aactggtctg cgctaaggca ctgctaaaat acatgataaa
     6961 gctataacac aaagcggcta acaataaccg atgattgcca aaacgatctc ctaattttcc
     7021 taagtaagaa ctgctaaata agctagaaaa ccccatggcc gaaactacca ggcctgaagt
     7081 aaacattaag ttgtgagttt gaccaagatg acgaatatac aatgataaaa tgggggccac
     7141 tgattgagct gaaatttgaa taatcatact agtcacaaac aaaccaagca taatctgggg
     7201 actttttacc tgttttaaaa tgacttttgt aggtatcatc tcaaagcgcc taatcggctt
     7261 aaactcttct ttaacataaa cagctgtcat taaagaacat aaaaataata tcacaccaac
     7321 taataagaaa acttgtcgaa ttcctaatag ctctgccaaa atccccccta gtaaaggacc
     7381 aattaaagcg cctgctgtca ctcctgttgc taatgtgcct aaagcataac ctgactcctc
     7441 ttttggggct tggctggcaa tcaaggcagt ggcatttggc acatagcccg caaagacacc
     7501 tgttaataat ctcaaaatga gtaaccaaaa cacattagga ataatagcta aaccacccat
     7561 ggtaaaggtc atgacaaaac tggctcgcag catcataggt tttcggccat aacgatcagc
     7621 caacttcccc caaacaggag caaataaagc tgaagcaagt gccgtcacag caaccgccag
     7681 accagcatag tattctacta attctgtcgg agtacctaaa ttttccacat atagagccat
     7741 aaaaggcata acaagtgaaa agcttgcccc agtaaagaag ttaccaagcc aagccacttt
     7801 tagattttgt cgccagttca ctgtcgttac cgctttatca ctaatagatc atcatcttct
     7861 ttccattggg cctttgatta ttataacaca tttctgccaa aaacagtgtc ccataatagt
     7921 gctaagtctc atctgatagc cttcatcgtt tacgtaaact acccagtaaa cctctgacca
     7981 gttctcgccc aattgttcta accgtcgtac taataaacgc atctgttgct ttttcgacga
     8041 ctgtttttcg tggacgccca acagaacgcc gattagcttt tttggtagct gcttgcgcct
     8101 tgatggcttc tttagcttct ttttcgcgtt gtgctgtcgc tatcgcttct tgggccagtc
     8161 ggttgtcttc taaaacctta gcagctaatt tttcataagc agattcacgg tcaatggact
     8221 gactatattt actggcaaaa ggtgaggact gcacaagctg ctgactctct atctctgaca
     8281 aaacagcaaa gctactctta ggcggcatga tataggcacg ttcaacgatg gatggttgtc
     8341 ccttatcatt caaaacagaa atcaaggcct caccgacttc tagttctgta atcacccttg
     8401 ccacatctaa gtcaggattt tgtcggaaag tatccgcagc gacacgaacc gccttttgtt
     8461 ctttcggtgt ataagcgcgc aaggcatgct gaatacgatt tccaagctga gccaaaaccg
     8521 tttctggcaa atcaagtggg ctttgagtca caaagaaaat cccaactccc tttgaacgaa
     8581 taagtcgcac aatctgttca actttttcca agaaaacttt aggggcatct ttgaagagca
     8641 agtgagcttc atcaaaaaag aaaaccatct taggtttgtc caaatcccca acttctggca
     8701 ataattcgta caactcagac aagagccaaa gtaagaaggt tgtgtaaagg gtaggagatt
     8761 gaaaaagctt agtcgctgac aaaatgttaa tggctccata gcccgaagca acatctaatt
     8821 gcatcaaatc agccacatct aaagcaggct ctccaaaaaa ttggtgggct ccttcttgtt
     8881 ctaatgtcaa gagacttctt tggatagccc cgatagattg cttggcgata ttaccgtaat
     8941 ggctggagta gtctgaggca tggtcaccaa cttcttttaa gatagcctgc aagtctttca
     9001 aatcaatcaa aagccagcct ttctcgtcgg cgattttaaa gacgatgttt aagacacctg
     9061 tctgggtatc attaagattc aataaacgcg acaacatcat cggtcccatt tcagaaatcg
     9121 ttgtccgtaa gggttgaccg ttttgaccaa aaacatccca cattcttaca ggaaaggcct
     9181 gaggctggta atcacttacc ccgattgtgg ctagacgcgc agccaattta tcagtcacct
     9241 ctcctgcttt agtaagatta gacaggtctc ctttgatatc tgccaaaaag actggcacac
     9301 cagctaaact taattgctct gctagcactt ttaaggtcac agttttacct gttccagtcg
     9361 ctccagcaat caaaccgtga cgatttagca tccctaattg taaggctatc ggttgattgc
     9421 cttttgcaat tgtcattgta gtcataggct tcctcttttt tgatgatcat acttgtcgat
     9481 atgtgtttac ccataagtta aagtgtttag aagggtctcg ctcttaatac tattataaca
     9541 tataaccctt gatgcgctaa tttaaaaggg accagctagt tttagacaga aatagcttga
     9601 ttttgacagc tatgatacga atctctcagc ttagctacct cggattggct aatgacaaca
     9661 acgcagattg tacttgcttt attaacgttt ccatgtctcc attattatca atcactagac
     9721 tggcataggg ctttttttcc tctattggta actgactggc tattcgctgc cttgcttttc
     9781 ctttgtctaa acgatttcta gccattaagc gctgcaattg tgtctgagca tctacataaa
     9841 ccagccaaat cgcatcaaac cagtcttggt agcccaattc cattaaaagg ggaatatcca
     9901 tgaaaaaaat ggcttggctc tgtgctaagt ggtcacgctt agccgccaat tcttccttaa
     9961 tgatttgatt ttgaatggct gaggatgttg ccatattatc tgggtttgaa aaaagcattt
    10021 ctgacagctt tgtgcgatct aactcaccat ctgcttttaa aatctgattc ccaaaagctt
    10081 ctctaagagc ctcgtaaagg cgaccgcctt tttcttgcaa gtcatggacc acttgatccg
    10141 catcaatcac ctgataacca gcttttctaa tgaccttaac cacagtcgat tttcctgatg
    10201 caatgcctcc tgtgatgcca ataatcatgg tctttttttc tggcattttg gacaaatgtg
    10261 cgtccccctt cctccaactt ttaatttgac aatagcctgt ccacaacgag gacacggttt
    10321 cccagtttga ccataaacct gcaaatagtc ctgcatggtg ccatcagctc ccaaagcatt
    10381 acggtaagta cggacggtcg aaccgccctt ttcaattcct aaagctagga ttcgtatggt
    10441 ttcatcatgc agccgtttta tctccgcctt attcaaccta gaagacgcag tttctggatg
    10501 aaccttagca gcccaaagta cctcgtccac ataaatattc cctaaaccag caaccaaggt
    10561 ctggtctaac agatgtggtt ttatgggttt ttgggaggat agcagagcag cctcaaaggt
    10621 ttttaactta aaggtctctt tctttggttc agggcctagt tttcgtttgg caaaaaacgc
    10681 tgataattga gattttgtta tcaaatcaaa ggttccaaat ttacgcacat cttggtaaac
    10741 caaggtcgaa ccatttttca attcaaaaaa gacatgaaag tgtttgttat cagggacctc
    10801 atctggaaaa agcaaatatt tgccttccat ccgcaaatgt gataccaaca ctaattgccc
    10861 caaatcaatt aataaatatt ttccccgacg gccaactgac tgaatgattt gaccaggtag
    10921 ggtcagggca aacgtctcta agtcggtttt aaccattttt ggcactttaa gcgtaacagc
    10981 cacaatttct tggccaagca ctaaagtctc aagccctcgt cggaccgttt caacttctgg
    11041 taattctggc ataatccgtt atttaaagga cagctagacc ctttgctgct acaaagaata
    11101 aactcagcat aaaaaagtca ctcaatagac tcaggtctag gatactgact gcgtttcaaa
    11161 aaacacagct gtccttctcc tttcttattt cttattttac gaacacatct gatagaaagc
    11221 aactcataaa tctgcctcag accagtcctc atccccctta cctcccagca ctatttggcg
    11281 gtaatgttcc ttataactat aatacaatga gtcctctcca agatacttaa aaatctgcaa
    11341 ggcctgccgc atgtcctctt ctccagacat ttcttcctgc tttaatttat aatacccatg
    11401 cacatataag aaaacagtct tttcataaaa attgagctcg tctcgcagca aaagatcaat
    11461 tttgttaatc aaatagcgac tatgttcaaa gcaagaatgg tcaatactga taatgagaca
    11521 attaatggat aactgagtca ctaacatttt attggtttta ttttgttccg aataagcaaa
    11581 agatgctacc atttccttag tcaataaaaa tagggtattg taattcatga agcgcgagca
    11641 attccccaga agaatgattt cataatatcc ccactgttcc actttaaata aataatctgt
    11701 gaggcgagtc aattcttctt gactaggtgc aattgaggag tcaatagaaa aaagaatagc
    11761 tttaatcatc gttcgttcat aatctttatg ggcataatcc ttcaacagtt tggtcaattt
    11821 aaccacgttt ttctcagcat aacattttct cgcctgactt aagagcgtga aaaaatgcgt
    11881 atgcgtcttg gaatgagccg aaacaaattc gtcaatcgta atattcagtt tatccaaaag
    11941 attaagcaag cgactgcagg tgatttctga ttcccctcgt tcaaatcttg aaatttgtga
    12001 cttggacagg tattcatctg ctaaaaaaga aatgctaacc tgcttaccct tacgtaatcg
    12061 ccttaaggtt tttccgagtt ctttttccat ttttcccact ttcacaacaa aaaattaaaa
    12121 tctgctaaat ccattatata atacaagtac aaaatagaaa atcagaaagg agacagtcat
    12181 gaaaaaagtt aataaagctt tgctttttac attgatcatg gatatcctga ttatcgttgg
    12241 tggataacct ttcgcttaaa aacaatccac ttttgggaaa cccttatatt tagtttgtaa
    12301 caggaaaact aatagatatt aaaaagagtg ttagcgacct agcggcagct ttactcttaa
    12361 gtgcgtttgc tgccattgac agcaaggtca aagctgatga aacagcaggg gcataccgtt
    12421 atgatgtgtc taaacaaaac acttactatc aaactatcag ctccattaac cagcaacgac
    12481 ctggtgccac tctcgacgaa tgaggtgatt attatggaac ccttacttca tttacttccc
    12541 aaagtcgtac cagtacttat acacttggta gacaaagcca acgtatcatg gttaacagtg
    12601 atattacagt tacctatact cttaaagaca atttggtagc ccttcataag gaatataaaa
    12661 cctttgtcaa aaaaatatta cagataaacc gatcaccagt gactacgaca aggctaaggt
    12721 tatttataac catttaatca acgaatacac ttacgccaca aaataactta gcaccaatcg
    12781 caaaaccgta agcagcatca acaatcatac tcctaaagct ctttatcgta gcaagtgtgg
    12841 tgtttgtcaa gcttatgccg tcatgtttaa agatatggca gaagctgcag gcttatcagc
    12901 ttggtatgtg ataaaaaagg attgaatgag caccatgctt gaaacatcgt taccattcat
    12961 ggtgttaatt cctacgttga tgctacctgg aatgattcag catacaccac aaggtatttc
    13021 cttgctggaa aagacgtgag gaatactgag taccgtttag atagccagta caaccacttg
    13081 gctaatgcta ttttaaatat cagctactaa aaaggctaaa ctaacaacaa ctacattttt
    13141 caactagaaa ggcctaaaca atgaccaaaa aacagtttta tcttatctta gctattagca
    13201 tactgtgttt tattcttgcc gtcagcccta aaccaccaat tcctaaacac actattgaaa
    13261 ggaaaaggac tgcgatgacc ataggccact caaaacaaaa ccctcacaaa aaagaagccc
    13321 ttaaacacaa aaaaaataaa aataaggctc tatttcttcc aagtggggac agcgctatca
    13381 aatctgttat tagcatgaca gttagactcc tgttagatga ccaatcttag ctctcctaaa
    13441 atcagtggac actaccattt cggtagtgtt cttttttaag ctaatctcat caccacgctt
    13501 tcttcaagct aaaaaactga tcatgaaaga gcaaccccat caccctaaca gcccactttg
    13561 cccgcacttt cttaaaaagt taggagaaaa cgatatacca attattttct gatcccagct
    13621 ccctatcatt aacaagatgt gtagcacgga cctacataca ggtaagctag agtcattctc
    13681 gctatttcgg acaaaaagag gccggtagtt ccctgacctc ttctttattt atcttgtaac
    13741 actcctcttc ttagcgagac ttactagcac ataattctta agcactctta tcatttagac
    13801 taggaagaac accatctgaa tgaagcaaat gatgcgtcag atctgccaga gtatcatctc
    13861 cataagtaat ccctgtttgg tgctgtttct gatgaaaatc agatgactcc ttaactttag
    13921 cagtcacctg ctggtcactt ggatgaaaat taatttctct cttggcttca gccgcaactg
    13981 gctcgaggaa caacttattg acattacggt cttcctccaa atgattaagg atagcagtat
    14041 catcttctaa cgaaagaacc ttcttgggta acaaaaaggg gttatttagc cttggctcag
    14101 taacgacttc cttggctact cgtgatagtt tattattgtc ttcctcaggt atcaaacggt
    14161 taggcaatcg atgcaattct tccggctatg aggcgctcac agctacttca gaagaaacct
    14221 ccaagccctt tcctgtttgc ctgtctaaag caaccggcaa attattaact aactttttaa
    14281 cctgtcctca gcgctcattt caacactagg aagagctgtc gctggttcta gccagctacc
    14341 aacttctgct ggtcttacct taacatcgct ggcaggcttc tccttctcat cttcattagg
    14401 tctaaaaggc tcaaaccgcc ctgttgattt ttcatctatg acctttatat tgtcttttac
    14461 tacatgagac agtggttcga catttaccat agcttcatgc tcgttaaggc tctggtctcg
    14521 tttgaattta ccaggaatac caggagtcgg tgttccgaca gattgatcgg cttcagtagg
    14581 ctcttttacg gagccttttt tccataaaac tgctggagga ataatatccg tagagccagc
    14641 cccataagta atcgtggcca gccccccccc ctttggatta atagatactt taatattgtc
    14701 cggtaaggct ccactacctc ctgtggttaa aagggcaccc gcatttttaa tgagagttgt
    14761 cattcgttca ataccagcaa cgccaatatg gttcaagtca tctactggta actgatcaac
    14821 agatttcatg gtgtatttcc aagcttgcgg ttgaacaaga aaaacagttc ctttatacat
    14881 tgtgttatgc atatcatcaa tgactttgaa ataccttgta tatttggttt gtacattagg
    14941 gttagcaacg ggaacagtgc tttcaggaac tcctgacatc gtaaaaatct ctctagcaga
    15001 agtttgaaag ccctcataaa catgacccat accaggaaca gtctgagcgg tcaaagactt
    15061 accagaacca tccaaagcaa tgcgaatatc tttcatcaga tacatctgat cggtataggt
    15121 aaacctaacc gtgttagcct cacctcgata caaaaccact tgggagggct gatccgaaaa
    15181 aaactgagag gttgtttctt ccttatagcc aactccaaaa cgtctatcag acgaatggac
    15241 tgaatctgcc gcaactgtac tactcaaact gactagtaaa gtagccgcac aaccaactaa
    15301 taaactgctc gttttaactt taacatctct taacatcatc atgttccttt cattattttt
    15361 ta
//
LOCUS       DATURV010000038        14859 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DATURV010000038 DATURV010000000
VERSION     DATURV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14859
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.038"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            45..749
                     /locus_tag="VCF75_001143"
     CDS             45..749
                     /locus_tag="VCF75_001143"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEP2546708.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENMIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVATHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKCWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            873..4598
                     /locus_tag="VCF75_001144"
     CDS             873..4598
                     /locus_tag="VCF75_001144"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEP2546709.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDITEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFENYKADDFTAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHSGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRLSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGEIIVDLERRFLDTNGVRVVVDVKVVDKDLTV
                     PEARTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKDNFSQFEAIQAQHKIT
                     AASAAKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQTDVLEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEVAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            4759..6213
                     /locus_tag="VCF75_001145"
     CDS             4759..6213
                     /locus_tag="VCF75_001145"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEP2546710.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPNFIGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCNRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     HLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6241..7263
                     /locus_tag="VCF75_001146"
     CDS             6241..7263
                     /locus_tag="VCF75_001146"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEP2546711.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGFASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     ASVVIK"
     gene            7431..7985
                     /gene="purN"
                     /locus_tag="VCF75_001147"
     CDS             7431..7985
                     /gene="purN"
                     /locus_tag="VCF75_001147"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEP2546712.1"
                     /translation="MKIAVFASGNGSNFQVIAEHFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLAGYMKIVGETLLLAYERRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8169..9716
                     /gene="purH"
                     /locus_tag="VCF75_001148"
     CDS             8169..9716
                     /gene="purH"
                     /locus_tag="VCF75_001148"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEP2546713.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLDWDIISTGGTKVALDDAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDVDAAIRIIRDFKDRPTVVVLKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKIHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEAIALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(9774..10898)
                     /locus_tag="VCF75_001149"
     CDS             complement(9774..10898)
                     /locus_tag="VCF75_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEP2546714.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFIVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            11152..12417
                     /gene="purD"
                     /locus_tag="VCF75_001150"
     CDS             11152..12417
                     /gene="purD"
                     /locus_tag="VCF75_001150"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEP2546715.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKIDGDIITYYAGAKFAENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDPTGLFYRNDIGSKAIRE"
     gene            12695..13186
                     /gene="purE"
                     /locus_tag="VCF75_001151"
     CDS             12695..13186
                     /gene="purE"
                     /locus_tag="VCF75_001151"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEP2546716.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            13173..14246
                     /gene="purK"
                     /locus_tag="VCF75_001152"
     CDS             13173..14246
                     /gene="purK"
                     /locus_tag="VCF75_001152"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEP2546717.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRISQNR
                     IVEKDFLANKAGVTVAPYKVVTSSLDLGGLDLTKTYVLKTATGGYDGHGQKIIRSAED
                     LPEVQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKTAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLE
                     AKHNRKMGHVTLFAEDADEVEEF"
     gene            14248..>14859
                     /locus_tag="VCF75_001153"
     CDS             14248..>14859
                     /locus_tag="VCF75_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546718.1"
                     /translation="MYLKYIFKIKDRNLVSDYESLVRNLTINLQKLVIKDRTALIGDK
                     QLPITIYYRGENEKFPTGIVKVESDSVEGLELFDKTIKEIVGNESRFGKFTCIYDGIS
                     TYYSTEIYSELHEIERRTQEFLREAFFLSDKSGELKEYQATIKKMDSSDFSFSKLINE
                     IFKASDITDTFIDHVRACVEFGTVPNGDLLPKSFWDSLDNSLLD"
BASE COUNT         4268 a         2917 c         3480 g         4194 t
ORIGIN      
        1 tatgttactc ttaaatgaaa acagaaagaa aggcgaataa tcaagtgact aaccagctta
       61 tttataaagg aaaagcgaaa gatatttata gtacaaaaga tgaaaatatg attagaacgg
      121 tctataaaga ccaagctact atgttgaatg gagctcgtaa ggagactatt gatggcaagg
      181 gagcactaaa taatcagatt tcatctctta tttttgagaa attaaataag gctggtgtgg
      241 cgacacatta tattgagcaa atttcaaaaa acgaacaatt gaataaaaaa gttgatatta
      301 ttccgcttga ggtggtgtta cggaatgtga cagcaggctc attttcaaaa cgttttggag
      361 tggaagaagg acgtgttctt gaaacgccaa ttgtggaatt ttactacaaa aatgatgatc
      421 tgaatgatcc ttttatcaat gatgagcatg tgaagttttt gggtattgtt aatgatgagg
      481 agattgctta tctgaaagga gagactcgcc gtattaatga actcttgaaa tgttggtttg
      541 ctcagattgg gcttaacttg attgacttca agttggagtt cggatttgat caagaaggta
      601 caattatctt agcagatgaa ttttcgcctg ataactgtcg cctatgggat aagaatggta
      661 accatatgga caaggatgtt tttagaagag accttggcaa cctgacggat gtctatcaag
      721 ttgtacttga gaagttgata gcattgtaac tagaagagga aggaattgaa tccgttcggg
      781 aaactccgaa aaaaagatgg aacttactga aatctcaatt tcagatgtcc tcctattttc
      841 cagtcgctcc cttgtcggac ttggtatctt atatgaataa acgtattttt gttgagaaaa
      901 aggctgactt tggtattaaa tcggctagtc ttgtgaaaga gttgacgcat aatctacaac
      961 tgacctcttt gaaggctttg cgtattgtgc aggtctatga tgtcttcaat ttggctgagg
     1021 atttgctggc gcgtgctgag aagcatattt tctctgagca ggtgacagac tgtcttttga
     1081 cggaaactga aatcactgcg gagcttgata aggttgcctt ctttgccatt gaggcgcttc
     1141 ctggtcaatt tgaccaacgt gctgctagtt cgcaagaagc tttgctatta tttggaagcg
     1201 acagtcaggt taaggtcaat acagcccagc tttacttggt caataaggat attacagaag
     1261 cagagcttga agccgttaag aactatcttt tgaaccctgt tgattcgcgt ttcaaggaca
     1321 ttactttgcc gcttgaagag caggctttct ctgtgtctga taagacgatc cctaatcttg
     1381 atttctttga aaattataaa gctgacgatt ttacggccta taaggcagag cagggcttgg
     1441 ctatggaggt cgatgacctt ctcttcatcc aagattattt caaatcaatc ggacgtgtgc
     1501 caactgagac tgagttgaaa gttttggata cttactggtc agaccactgc cgtcacacaa
     1561 cctttgaaac tgaattgaag aacattgatt tttcagcttc taaattccaa aaacaattgc
     1621 agacaactta tgacaaatat atcgccatgc gtgatgagct tggtcgttct gaaaagccac
     1681 aaacacttat ggatatggcg actatttttg gtcgttatga gcgtgccaac ggtcgtctgg
     1741 acgatatgga agtctcagat gaaatcaatg cctgctcagt tgagattgaa gtagatgttg
     1801 atggtgtgaa agagccttgg ctcctcatgt ttaagaacga gactcacaat cacccaacag
     1861 aaattgagcc attcggtgga gcggcgactt gtatcggtgg tgctattcgt gacccattgt
     1921 cagggcgttc atacgtttat caggctatgc gtatttcagg cgcaggcgat atcacgactc
     1981 cgattgcgga aacacgtgct ggtaaattgc cacaacaagt tatttctaaa actgcggcgc
     2041 acggctattc ttcatatggt aaccaaattg ggcttgcgac aacttatgtg cgtgagtact
     2101 tccactctgg ctttgtagcc aaacgtatgg agcttggagc tgtggttggt gctgcaccta
     2161 aggaaaatgt ggttcgtgaa aaaccagaag caggtgatgt ggtcatcttg ctcggtggta
     2221 aaacaggtcg tgatggtgtc ggcggtgcga caggttcatc taaggttcaa acggttgaat
     2281 ctgtggaaac agcaggagca gaggtacaaa aagggaatgc catcgaagag cgtaagattc
     2341 aacgtctttt ccgtgatggc aatgtcactc gtctgatcaa gaaatcaaat gacttcggtg
     2401 caggtggtgt ctgtgttgcc atcggtgaat tggctgacgg tcttgaaatc gatttggaca
     2461 aggtgcctct taaataccaa ggtcttaatg gtactgaaat tgcaatctca gaatctcaag
     2521 agcgtatgtc agtcgttgtt cgtctaagtg atgtggatgc cttcatcgca gcctgcaaca
     2581 aggaaaatat cgatgcagtc gttgttgcga ccgttactga aaaaccaaat cttgtcatga
     2641 cttggaatgg cgaaatcatc gttgatttgg aacgccgttt ccttgatacc aatggtgtcc
     2701 gtgtcgttgt tgatgttaaa gtcgttgaca aggacttgac agttccagaa gcacgtacaa
     2761 catcagcaga gacacttgaa gcagatacgc ttaaggtctt gtctgacctc aaccacgcta
     2821 gtcaaaaagg tctccaaacc atctttgact catctgttgg tcgttcaacc gttaaccacc
     2881 caatcggtgg tcgttaccaa atcacaccga cagaaagttc tgttcaaaaa ttgccagttc
     2941 aacatggtgt gacaacaact gcatctgtta tggctcaagg ttacaatcct tatattgcag
     3001 agtggtcacc ttatcacggt gctgcctatg ctgtcattga agcgacagct cgcttggtag
     3061 caacgggtgc tgactggtct cgtgcacgtt tctcttacca agagtacttt gagcgtatgg
     3121 ataaacaggc agagcgtttt ggtcagccag tatcagctct tcttggttct attgaggctc
     3181 agattcaact tggtttgcca tcaatcggcg gtaaggactc tatgtctggt actttcgaag
     3241 acttgacagt accaccaacc ttggtagctt tcggcgtgac aacagcggac agccgcaagg
     3301 ttctctctcc tgagtttaaa gcggctggcg aaaacattta ctatatccca ggtcaagcta
     3361 tttcagaaga tattgatttt gaccttatca aggataactt tagccagttt gaagctattc
     3421 aagctcaaca taagattaca gctgcctcag ccgctaaata cggtggtgtc ctagaaagtc
     3481 ttgctctcat gacttttggt aaccgtatcg gtgcttctgt tgaaattgca gagcttgaca
     3541 gcagcttgac agctcaactc ggaggttttg tctttacatc agctgaggaa attgctgacg
     3601 cggtgaaaat cggtcaaact caggcagact ttacagtcac agtcaatgga aatgaccttg
     3661 ctggcgctag ccttctagca gccttcgaag gcaaattgga agaggtttac ccaacagaat
     3721 ttgaacagac agatgttctt gaagaagttc ctgctgtggt atcagatact gttatcaagg
     3781 ctaaggaaac aattgaaaaa ccagtggttt acattccagt cttccctggt accaactcag
     3841 aatacgattc agctaaggcc tttgaacagg ttggagctag tgtcaacttg gtaccatttg
     3901 taaccttgaa tgaggttgct attgctgagt cagttgacac tatggttgct aatattgcta
     3961 aggcaaatat catcttcttt gctggaggtt tctcagcagc ggatgaacca gatgggtctg
     4021 ctaagtttat cgtcaatatc ttgcttaacg agaaggtccg cgcagctatt gacagcttca
     4081 tcgaaaaagg tggccttatc atcggtatct gtaatggttt ccaagccctt gttaaatcag
     4141 gtcttcttcc atacggaaac ttcgaggaag ctggtgagac aagtccaact ctcttctata
     4201 acgatgctaa tcagcatgtt gccaagatgg ttgagactcg tatcgcaaat accaactcac
     4261 cttggttggc aggagttgag gtcggcgata ttcatgccat tccagtttca catggtgaag
     4321 gtaaatttgt tgtcagcgct tctgaatttg cagagctaag agacaatggt caaatctgga
     4381 gccaatatgt ggactttgac ggacaaccat ctatggattc taaatacaat ccaaacggct
     4441 ctgtcaatgc catcgaaggg attaccagca agaatggtca aatcatcggt aagatgggac
     4501 actcagaacg ctgggaagac ggactcttcc aaaatatccc tggtaacaaa gaccaaaccc
     4561 tctttgcaag tgctgtaaaa tactttacag ggaagtaaaa aggttcagtg aaccttttta
     4621 gccctcgcct tgaattagtg aagggcgagg ttagaaagaa tctcgcttaa tttttgagaa
     4681 atagtaaaca cgggcgataa gttgtgcgaa aaactttcta ttttcgcttt acttgacagc
     4741 ctttgtatcg aggtaaaaat gacatacgaa gtaaaatctc taaatgaaga atgtggagtc
     4801 tttggtatct ggggacatcc tcaggcagct caagtcactt actttgggct tcatagcctt
     4861 caacatcgcg gtcaagaagg agctggtatt gtttcaaatg acaatgggaa actctatggt
     4921 tatcgaaatg ttgggctcct ttctgaagtt tttaagaatc aatctgaatt agataattta
     4981 actgggaatg cggctattgg acatgttcgg tatgctactg caggttctgc agatattcgc
     5041 aatattcagc cttttcttta taaatttcat gacgggcaat ttgctttatg ccataatggt
     5101 aatttgacaa atgctatttc cttaaggaaa gaattagaaa agcaaggtgc aattttcaat
     5161 gcctcctcag atactgaaat tttaatgcat ttgattcgtc gaagccataa cccaaatttc
     5221 ataggaaagg taaaagaagc tctaaataca gtaaagggtg gctttgccta tctactgatg
     5281 acagaaaata aactcattgc tgctcttgac cctaatgcct ttcgtccttt gtcaattgga
     5341 caaatgcaaa atggtgcctg ggttatttcc agtgagacct gtgcttttga ggtggtaggc
     5401 gcaaaatggg ttagagatgt tgaacctggc gaagttattc ttatagacga tagtggtatt
     5461 caatgtaatc gttatactga tgaaacgcaa ctggcaattt gttcgatgga atatgtctat
     5521 tttgcaaggc cggattcaac tatacatggt gttaatgttc atacggctcg aaaaaatatg
     5581 ggaaagcatc ttgcacaaga atttaaacag gatgctgata ttgtaattgg tgtcccaaat
     5641 tcgtccttat cggctgctat gggctttgct gaagaatccg gattaccaaa tgagatgggt
     5701 cttgtaaaaa atcagtatac gcagcgaacc tttattcaac cgacacaaga attaagggaa
     5761 caaggtgttc gaatgaaact atcagcggta tctggtgttg tcaaaggaaa gcgcgttgtt
     5821 atgattgatg actcaattgt aagaggaacg acttctagaa ggattgtcgg attattaaga
     5881 gaagcaggag ctagtgaagt acatgttgct atagctagtc cagaattaaa gtatccttgt
     5941 ttttatggta ttgatattca aacaagacga gaattaattt cagcaaatca cgctgctgac
     6001 gaagtgtgtg atattattgg cgcagatagt ctgacctatc tctcactaga tgggttgatt
     6061 gaatcaattg gactcgaaac aaaagcacca aatggtggtt tatgcgtagc ttactttgat
     6121 ggacattatc caacaccgct ttatgattat gaagaagaat acctcagaag tttagaagaa
     6181 aaaacaagtt tttatattca aaaagtgaaa taagctctgc taattatata aggagaaaac
     6241 atgtctgaaa aaaatgctta tgcaaaatct ggtgttgatg ttgaagctgg ttatgaagtg
     6301 gttgaaagaa taaaaaaaca cgttgctcgt acagaacgtg cgggagtcat gggagctcta
     6361 ggtggctttg gtgggatgtt tgacttgagt aaaacaggag ttagagagcc tgttttggtc
     6421 tcagggacag acggtgttgg cacaaaactc atgcttgcta tcaagtacga caagcacgac
     6481 acaatcggtc aagactgtgt tgccatgtgt gtcaatgata ttattgcagc aggtgctgag
     6541 cccctttact tccttgacta cgtagcgaca ggaaaaaata atccagtcaa atttgaagaa
     6601 gttgtttctg gcgttgcaga aggttgtgtt caagcaggag cagcactcat tggaggtgag
     6661 accgctgaaa tgcctgggat gtatggcgaa gatgattatg accttgctgg ctttgctgtt
     6721 ggtgtggctg aaaaatctca gctcatcgac ggttcaaaag taaaggaagg cgatattctt
     6781 ttgggatttg cttcaagtgg tatccattca aacggttatt ctttggtacg tcgtgtcttt
     6841 gctgactata ctggtaaaga gctgcttcca gaacttgaag gaaaacaact taaggatgtc
     6901 cttcttgagc caactcgtat ttatgttaga gcagctctgc cattaatcaa ggaagaacta
     6961 gtcaacggta tcggccacat cacaggtggt ggctttatcg agaatgttcc tcgtatgttt
     7021 gcagatgatt tggctgcgga aatcgatgag gacaaggtgc cagtgcttcc gattttcaag
     7081 gcgcttgaaa aatatggtga catcaagcac gaagaaatgt ttgaaatctt caatatgggt
     7141 gtcggtctta tgctagctgt tagtcctgaa aatgttaacc gcgtcaaaga gctcttggat
     7201 gaaccagttt atgaaattgg tcgtatcatc aagaaagcag acgctagtgt ggtgattaaa
     7261 taagtcacta aattttataa gaaaggagtg tttagttagt ttgcctaact gaacacggga
     7321 ttaaatttct aggaaaaaag ataaaatgac ttagacgcaa gcgtcgttgt tcgttttcct
     7381 atttttctac gaaatttttc ggcaagccga atcatccctt tgtatcgaat atgaaaatcg
     7441 ctgtttttgc ttctggtaat ggttccaact ttcaggtcat agcagagcac tttccagtta
     7501 gttttgtctt ttcagatcat cgtgatgcct atgttttaga gcgtgctcag aatttagcca
     7561 ttccaagctt cgcttttgaa ctcaaggagt ttgagaataa ggcggcttac gagcaagcca
     7621 tcgttgattt gttggacaaa cacgagattg acttagtctg tctggcgggc tatatgaaga
     7681 ttgttggaga aaccttactc ttggcctatg agaggcgtat tatcaatatt cacccagcct
     7741 acctgcctga atttccaggt gcccacggta tcgaggatgc ttgggaagct ggtgttgacc
     7801 agtctggcgt gaccatccac tgggttgact ctggtgtgga taccggtcag gtcatccaac
     7861 aagtgcgcgt gccacgccta gcagacgata gcctagaaag ctttgaaacc cgtatccatg
     7921 aaaccgaata tcaactctac ccagctgtct tagatagctt gggagtggag aggaaagtaa
     7981 tctagccttt atcttaaaat tttaggtaaa gttaggaaag atagtaaaga agaattaacg
     8041 ttccaaaaag aaaggaattg aacccgtctg gaaggcacag aaaaaagata aacatctcct
     8101 aggcgcaagc gtcttcgtcg agtttcctat ttttccttgg ccttctagtc ggacttggta
     8161 tcttaaatat gactaaacgt gctttaatct cagtgtctga caagtcagga attgttgact
     8221 ttgcaaaaga attgaaaaac ttggattggg atattatctc aactggtggg actaaggttg
     8281 cccttgacga tgctggtgtt gagaccattg ccatcgacga tgtgactgga ttcccagaga
     8341 tgatggacgg tcgtgttaag accctccacc caaacattca cggtgggctt ttggctcgtc
     8401 gcgacgctga cagccacctt caggctgcta aggacaacaa tatcgagctg attgacctcg
     8461 tggttgtcaa cctctatccc ttcaaggaga ctatccttcg tccagacata acctatgatt
     8521 tggcggtgga aaacatcgac atcggcggtc catcaatgct tcgctcagcc gctaaaaatc
     8581 acgctagcgt gaccgttgtg gttgacccag cggattatgc gactgttttg ggagaattgg
     8641 ctgacgctgg tcagacgaca tttgaaacgc gtcaacgctt ggcagctaag gtctttcgtc
     8701 acacagcagc ctacgacgct ttgattgctg agtacttcac aactcaagtg ggagaggcta
     8761 agcctgaaaa attaaccatc acttacgacc ttaaacaggc tatgcgttac ggagaaaatc
     8821 cacaacaaga cgctgatttc tatcaaaaag ccttgccaac agactactca attgcttctg
     8881 ctaaacagct caacggaaaa gaattgtcct tcaataatat ccgtgatgtt gatgcagcaa
     8941 tccgtattat ccgtgatttc aaagaccgtc cgacagtggt tgtcctcaaa cacatgaacc
     9001 catgtggtat cggacaggct gatgatattg agactgcttg ggattacgct tatgaagctg
     9061 acccagtttc aatctttggt ggaattgtcg tccttaaccg tgaagttgac gcagcgacag
     9121 ccaagaagat acaccctatc tttttggaaa tcatcatcgc accatcatac tcagaagaag
     9181 cgctagctat tctcacaaat aaaaagaaaa acttgcgaat ccttgagttg ccgtttgatg
     9241 cccaagctgc cagcgaagtg gaagctgagt acactggcgt agttggtgga cttttggtgc
     9301 aaaaccaaga cgttgtggct gaaaatccat ctgactggca agtggtgaca gaccgccaac
     9361 caacagaaca agaggcgatt gcccttgagt ttgcctggaa ggctatcaag tatgttaagt
     9421 ctaacgggat tattattact aacgatcaca tgacgcttgg actcggtgca ggtcaaacca
     9481 accgtgtcgg ctcagtcaag attgctatcg agcaggctaa agaccacctt gacggtgccg
     9541 ttctagcatc agatgccttc ttcccatttg cggacaacat tgaagaaatc gctgccgcag
     9601 ggatcaaagc aatcatccaa ccaggtggtt cagttcgtga ccaagactct attgacgccg
     9661 caaacaaaca tggcttgacc atgatcttca caggtgtgag acattttagg cattaataaa
     9721 aaaatgtggt ctaattaaaa ccacattttt tattaaaaga aaatataagt taactataat
     9781 ttgttagctt tatcagcatt tacccatgtt ccacgagatc cgatttgaac ataaatacca
     9841 ttacttggtt gatcaatttt taatacttta taactaccag tgacgataaa atattcaccc
     9901 acacggagaa tttgatctcc taaaactttt ccttggttat ctgtttcatc taatggtgtg
     9961 ggatcaatcc agtttagtgg agttgggtct cctccggcta attctttatt aacgatcaaa
    10021 ttattaacaa gctgatctac acgaaaaacg ccagggaagg taacagtatc accgacctta
    10081 atggaaggtt ttgtattgtc attttgaacc acagactgtg cgggctcttt aatgggaata
    10141 tagcgtcggt ttccagaaaa actgaggtag ctaagccatg tataacctcc agcagtcacg
    10201 actttatcgt aataaacaga ttgacctgct tcatagtaag caagatcagg gctatcgata
    10261 ctggtttgtc ctttgactgg taatctggtt gtaaagtgat aagttccaga ggggtcaaat
    10321 gaagcttgag aaatgtgttt tagttgtctt gggtaggaat gacttgtctg agatgataag
    10381 tctttaaaat ggatataacc acttacctga gattttggaa tttgacgctt atggtatctt
    10441 tcagggcctt gtccagcgtt gtaattatac tcttcgatag tgacagtgtc tccacggata
    10501 tcagctaccc atgctacatg accgtaagca gcatttgact gataagcgtt tttatcaaac
    10561 caagcgatag cccctatgct tggtgtctta ttcacaggat aaccctgatt tttcgcgata
    10621 tgtccccacg tgcaggcatt accgtagcct ttaggtaact gaaaaccatt agcagagctt
    10681 aaacgaaaag ctgcaaaaga agtgcattgg cggatataca tgttccacga atcgattcca
    10741 ttgccttttt tccacttact cggatagtta tcgcctaaaa cagctgcatg aacaagattt
    10801 tcctgttgcg aaataagtgt agatggcata gtaggtacta gaccattaaa acctaaaagg
    10861 attactcctg agaccaaaaa acgatgaaat tttttcatta attacctcct gtaacttatt
    10921 attcgaaagc aagaaacaaa aaatgttatt tttagatgat tattttgaaa attggcttta
    10981 cttgatatta aataactgtt tgattggcta atagagtgtt ccttatttca agaattgtgg
    11041 tctactatca catttttaca atgtttcaat tattgataac taatacatta tgtaaaaaaa
    11101 gcgaacgttt atgatataat atctaattat aatacgaaag aggtggatgt tttgaaatta
    11161 cttgttgttg gttcaggtgg gcgtgaacat gcgattgcta agaagttgtt agcgtctaag
    11221 ggtgtggatc aggtttttgt ggcacctggt aatgatggta tgaccttgga tggtctggac
    11281 ttggtgaata tagtagtttc cgaacattct aggttgattg cctttgctaa ggagaatgag
    11341 atttcttggg cctttattgg tcctgatgat gcgctagcag ctggtatcgt tgatgatttc
    11401 aacagtgctg gactcagagc ttttggtcca acaaaggcag cagcggagct agagtggtca
    11461 aaagactttg ccaaggaaat catggtcaaa tacaatgtcc caacagcagc ctatggcaca
    11521 ttttcagact ttgaaaaagc caaagcctac atcgaagagc agggcgcacc tatcgtggtc
    11581 aaggctgatg gcttggcgct aggtaagggt gtggtcgtgg ctgaaaccgt tgagcaggcg
    11641 gtagaggcgg cgcaagagat gcttctggac aataagtttg gcgactcagg agcgcgtgtg
    11701 gttatcgagg aattcttgga tggcgaggag ttttccctct ttgcctttgt taatggtgat
    11761 aagttctaca tcatgccgac agctcaggat cacaagcgtg cctttgacgg agacaagggg
    11821 ccaaacactg gtggtatggg tgcctatgcg cctgttcccc acctgcctca gagcgtggtg
    11881 gatacagcgg ttgagaccat cgttaagcct gtccttgaag gtatgattgc cgaagggcgt
    11941 ccttatctag gcgtcctcta tgcggggctt attctgacgg ctgatggccc taaggttatt
    12001 gagttcaact cacgtttcgg tgaccctgaa actcagatta tcctccctcg cctgacttct
    12061 gatttcgctc agaacatcga cgatatcatg atgggcattg agccttacat cacttggcag
    12121 aaggacggcg tgactctggg cgttgtcgtt gcctcagaag gctatccgct cgattacgag
    12181 aaaggtgtgc cactgcctga aaagatcgac ggcgacatca tcacctacta tgctggagct
    12241 aagtttgcgg aaaatagcga gctacttctc tcaaacggag gacgtgtcta tatgcttgtc
    12301 accacagaag acagcgtcaa agcagggcag gacaaaatct atacccagct agcccaacaa
    12361 gacccaacag gtctcttcta ccgaaatgac atcggaagca aagctataag agaataagac
    12421 taaccattca aatattagcg tcagcaatca acgaccgaag gtcgctccaa aaagaaagaa
    12481 gaaaggaact gaacacggga ctaatttcct aggaaaaaaa taaaactacc tggacgcaag
    12541 cgtcatcgtt agttttctgc gtcgtaggcg tctgatgcta aagcacctag tcgccctgac
    12601 gtgggttcgt cttcgaaatc aaagatttcg gacttaccgc atatttttct acggaaattt
    12661 tcgacaagtc gaaacgcccc tttgtatctt aattatgaaa actccaatta tttctattat
    12721 catgggctcc aaatctgact gggcaaccat gcaaaaaacg gctgaagttc tagacaactt
    12781 tggcattgct tacgagaaga aagtcgtctc tgcccaccgt acgccagacc tcatgttcaa
    12841 gcatgctgag gaagcacgtg gtcgtggcat caaaatcatc atcgcaggag caggtggggc
    12901 agctcacttg cctggtatgg tggcagctaa aacaaccctg cctgttatcg gtgtgcctgt
    12961 caaatcacgt gccctttcag gtttggattc actctattcg attgtgcaga tgcctggcgg
    13021 tgtgcctgtg gcaaccatgg ctatcggaga agcgggagcg actaacgctg ccctgacagc
    13081 cctccgtatt ctctccatcg aggatcaaaa tctggctgat gcgctagctc atttccatga
    13141 ggaacaagga aaaatcgcag aggagtcaag tggtgaactc atttaagacc attgggatta
    13201 tcggtggtgg tcagctgggg cagatgatgg ccattgcggc tatctatatg ggccacaagg
    13261 tcatcacgct ggatcctgct agcgactgcc ctgcctcccg cgttagcgag gtaattgtgg
    13321 cgccttacga tgatgttgag gctttgggac aattggctgc gcgttgcgat gtattgactt
    13381 atgagtttga gaatgttgat gcggacggtt tggatgccgt tgtgtcagct tgtcagctac
    13441 cgcaggggac cgatctgctc cgcatttccc aaaaccgtat cgttgaaaaa gactttctgg
    13501 caaataaggc tggtgtgaca gtcgctccct ataaggtggt gacgtctagc cttgacctag
    13561 ggggacttga tttgaccaag acctatgtcc tcaagacagc gacaggtggt tatgacggtc
    13621 atgggcaaaa gattatccgc tcagcagaag atttgccaga ggtgcagcaa ttagctaact
    13681 cagctcagtg tgtcttggaa gagtttgtca atttcgacct tgaaatctca gttatcgtgt
    13741 ctggaaacgg agaggatgtg acggtctttc ccgttcagga aaatatccac cgcaacaata
    13801 tcctgtcaaa aaccatcgta ccagctcgca tctcagatca actggctgat aaggctaaga
    13861 aaacggcggt gcagattgcc aagaaactcc agctgtcagg aactctctgt gtggaaatgt
    13921 ttgcgaccgc agatgatatc attgtcaatg aaattgcccc acgtccccac aactcagggc
    13981 actactcaat cgaggcttgc gacttttcac agtttgacac ccacattttg ggcgtgctag
    14041 gagcgccgct cccaactatc cacctccatg ctccagccgt catgctcaat gtcctcggac
    14101 aacacgtcca gcaggcgatt gactatgtga ctgaaaaccc tagcgcccac ctccacatgt
    14161 atggtaaact agaagcgaaa cataaccgca aaatgggaca tgtgacacta tttgcagaag
    14221 atgcggatga ggtggaggag ttttaggatg tatctaaagt atatatttaa aataaaggat
    14281 aggaatttag tatcggatta tgaatcttta gttcgaaatt tgacaatcaa tcttcaaaaa
    14341 ctagttatta aagatagaac tgcattaata ggggataaac aacttccaat tacgatatat
    14401 tatcgtgggg agaatgaaaa gtttcctacg ggaatagtaa aagttgaatc agactcagtt
    14461 gaaggattag agttatttga taaaacgata aaggaaatag ttgggaatga aagtagattt
    14521 ggaaagttca catgtatata cgatggaata agtacatatt attcaacaga aatttattct
    14581 gaacttcatg aaattgagcg tcgaacacaa gagtttttaa gagaagcatt ttttctgagc
    14641 gataaaagtg gagaattgaa agaatatcag gcaactatca aaaaaatgga tagtagtgat
    14701 ttctcattca gcaaattaat taatgaaatt tttaaagcaa gcgatataac tgatacattt
    14761 attgatcatg ttagagcgtg tgttgaattt ggaacagttc caaatggaga tttattacca
    14821 aaaagttttt gggatagttt agataactct ttactagat
//
LOCUS       DATURV010000039        14597 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DATURV010000039 DATURV010000000
VERSION     DATURV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.039"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            83..1447
                     /gene="ftsA"
                     /locus_tag="VCF75_001154"
     CDS             83..1447
                     /gene="ftsA"
                     /locus_tag="VCF75_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEP2546719.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            1471..2790
                     /gene="ftsZ"
                     /locus_tag="VCF75_001155"
     CDS             1471..2790
                     /gene="ftsZ"
                     /locus_tag="VCF75_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEP2546720.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            2793..3463
                     /locus_tag="VCF75_001156"
                     /pseudo
     CDS             2793..3463
                     /locus_tag="VCF75_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
     gene            3475..4131
                     /locus_tag="VCF75_001157"
     CDS             3475..4131
                     /locus_tag="VCF75_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEP2546721.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            4136..4390
                     /locus_tag="VCF75_001158"
     CDS             4136..4390
                     /locus_tag="VCF75_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEP2546722.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            4390..5181
                     /locus_tag="VCF75_001159"
     CDS             4390..5181
                     /locus_tag="VCF75_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEP2546723.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            5191..5949
                     /locus_tag="VCF75_001160"
     CDS             5191..5949
                     /locus_tag="VCF75_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEP2546724.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSMTEPEVNLSETQTFKLNI"
     gene            6222..9023
                     /locus_tag="VCF75_001161"
     CDS             6222..9023
                     /locus_tag="VCF75_001161"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEP2546725.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQESN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRNDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNTKENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGIAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(9288..9590)
                     /locus_tag="VCF75_001162"
     CDS             complement(9288..9590)
                     /locus_tag="VCF75_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEP2546726.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(9641..10096)
                     /locus_tag="VCF75_001163"
     CDS             complement(9641..10096)
                     /locus_tag="VCF75_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP2546727.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(10224..12506)
                     /locus_tag="VCF75_001164"
     CDS             complement(10224..12506)
                     /locus_tag="VCF75_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP2546728.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPTNSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            12805..13035
                     /locus_tag="VCF75_001165"
     CDS             12805..13035
                     /locus_tag="VCF75_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEP2546729.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            13162..13848
                     /locus_tag="VCF75_001166"
     CDS             13162..13848
                     /locus_tag="VCF75_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP2546730.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISISVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMITTVMAQLLAVL
                     ERHMAQGGNH"
     gene            13848..14582
                     /locus_tag="VCF75_001167"
     CDS             13848..14582
                     /locus_tag="VCF75_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2546731.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
BASE COUNT         4481 a         2511 c         3108 g         4497 t
ORIGIN      
        1 tggtataata ttttctgaat gattctgttt tttagcagtt tttagaatag caaaagtttg
       61 gaaagtaagt gaggtcaagt gaatggctag aaatggcttt tttactggtt tggacattgg
      121 aacaagctcg ataaaagttt tagtagcaga atttatttct ggtgagatga acgtcattgg
      181 tgttagtaat gttccaagta ccggcgtaaa agatggcata ataatcgata tagaggcagc
      241 tgcgactgcc atcaaaactg cggtagaaca agcagaagaa aaagcaggga tgacaattga
      301 aaaggttaat gttgggctac cggcaaacct tcttcaaatt gaaccaacac aaggaatgat
      361 tcctgtccca agtgagtcta aagagataaa agatgaggat gttgatagcg ttgttaaatc
      421 ggctttaaca aaaagtatca caccagaacg agaggttatc tctttagttc cagaagagtt
      481 cattgtggat ggctttcagg gcattcgaga tccacgtggt atgatgggga ttaggttaga
      541 gatgcgcggg cttatttata ctggaccaag caccatttta cataatctgc gtaaaacggt
      601 agaaagagca ggcattaaag ttgaaaacat cattatttct ccgttagcta tggctaaaac
      661 cattttaaac gaaggtgagc gcgagtttgg agctactgta attgatatgg gaggtggaca
      721 gacaactgtc gcttctatgc gagcacaaga attgcagtat accaatatat atgccgaagg
      781 cggcgaatac attactaaag atatatcaaa agtattaaaa acgtctttgg ctattgcaga
      841 agcacttaag tttaattttg gtcaagcgga gatatcagaa gctagtataa ctgaaacagt
      901 aaaagttgat gtggtaggta gtgaagagcc tgttgaggta actgaacgtt atttatctga
      961 aattatttca gcgcgtattc gtcatatttt agatcgtgtg aagcaagatt tggaaagagg
     1021 tcgtttacta gacttaccag gaggcattgt tttgattggt ggcggtgcaa tcatgcctgg
     1081 agtggtagaa attgcacaag aaatctttgg agtaactgta aagctccatg ttccaaatca
     1141 agtcggtatt agaaatccaa tgttttcaaa cgttatcagt ttggtagaat atgttggtat
     1201 gatgtctgaa gtagacgttt tagcacaaac tgcagtttca ggagaagaac ttttgcgacg
     1261 caagcctatc gatttcagtg gtcaagaatc ttatttacca gattatgatg attcaagaag
     1321 accagaatcg accattggct atgaacaaca agcgtcacaa acagcatatg attcacaagt
     1381 tccgagtgat cctaaacaaa aaatttcaga acgtgttcgt ggcatatttg ggagtatgtt
     1441 tgattaaaag taataaagtg aggaaataaa atggcatttt catttgatac tgcatcaatt
     1501 caaggtgcaa ttataaaagt aattggagtc ggcggaggtg gcggaaatgc cattaatcgt
     1561 atgattgatg aaggtgttgc tggtgttgag ttcatcgcag caaatacaga cattcaggca
     1621 ttaagctcat caaaagctga aacggttatt caactaggcc ctaaattaac tcgtggactt
     1681 ggtgctggag gacaacctga agtaggacgt aaagctgctg aagaaagcga agaaatttta
     1741 acagaagctc ttacaggagc ggacatggta tttattactg ccggtatggg tggtggctct
     1801 gggacagggg ctgcaccggt tattgctcgt atcgctaaaa gtttgggagc cttgacagta
     1861 gctgttgtta ctcgcccgtt tggttttgaa ggtaacaaac gtggtaattt tgctattgaa
     1921 ggtatcgaag aactacgtga acaagttgat actttgttaa ttatttcaaa taataacctt
     1981 cttgagattg ttgataaaaa gacaccttta ttagaagcac ttagtgaagc tgataatgtt
     2041 ttacgtcagg gagttcaagg gataaccgac ttaattacta gtcctggcct tatcaatctc
     2101 gattttgccg acgtgaaaac agttatggca aataaaggga atgccttgat ggggattggg
     2161 attggttctg gagaagagcg cattgttgag gcggcgcgta aggcaatata ttcaccccta
     2221 ttagaaacga ctattgatgg tgcacaagac gttattgtga acgttacagg aggtctcgac
     2281 atgacactta cagaagctga agaagcctct gaaattgttg ggcaagctgc tggtcaaggc
     2341 gttaacattt ggttaggaac atctattgat gatactatga aagatgacat ccgtgtgact
     2401 gtcgtagcaa ctggagtgcg ccaagaaaaa gccgaacaag tttcaggttt tcgtcagcct
     2461 aggactttta cccaaaccaa cgcgcagcaa gtagcgggtg cacaatatgc atcagatcaa
     2521 gcaaaacagt cggttcaacc agggtttgat cgtcgctcaa attttgattt tgacatgggg
     2581 gagtctcgcg agataccaag tgcacaaaag gtaatttcta atcataatca aaatcaaggt
     2641 tcagcttttg gaaattggga tttgagacgt gataatattt ctcgtccaac agaaggtgaa
     2701 ttagataacc atcttaatat gtcaacgttc tcagctaacg atgacagtga tgatgaatta
     2761 gaaacgcctc cattctttaa aaaccgttaa taatggattt actgacaaat aaaaagaaga
     2821 tttttgagac tatccgctta tctacagagg cagcaaatag gactaatgat agtgtttcag
     2881 ttattgctgt aacaaaatat gtggatagta caattgcagg tcagcttatc gaagcaggaa
     2941 ttgagcacat tgccgaaaac cgtgttgata aatttcttga aaagtatgat gcgttaaagt
     3001 atatgccagt aaagtggcat ttaatcggta ccttacaacg tcgtaaggtt aaggaagtta
     3061 tcaattatgt tgattatttt cacgctctag attctgtgag attagctttg gaaatcaaca
     3121 agagagctga ccatcctgtg aagtgttttc tacaagttaa tatttctaaa gaagagagta
     3181 aacatggttt taacatttct gagattgatg aagcgattga agaaataggt aagatggaga
     3241 agatacagtt agttggttta atgactatgg caccagcaaa tgccagtaaa gaaagtatta
     3301 taactatttt tcgacaagca aatcaattaa gaaaaaactt gcagttaaaa aaagaaagaa
     3361 tatgcctttt acagaattga gcatgggcat gagtaacgat tatccaattg ctattcaaga
     3421 aggctcaact tttattcgga ttggtagagc tttctttcac taatggagaa taagatggct
     3481 tttaaagata catttaacaa gatgatttct tattttgaca cggatgaggt taacgaagtt
     3541 gaagaagatg ttgcagcatc aactgataac gttattccaa gatcacaaca atcagtcaga
     3601 gcaagtagtc atccaaaaca agaacctaga aacaatcacg tacaacaaga tcatcaagcg
     3661 agatcccaag aacagacaag gtcacaaatg catccaaaac atggtacttc tgaacgctat
     3721 tatcagcagt ctcagccaaa agaaggccat gaaatggttg acagaagaaa acggatgagc
     3781 acttctggta ttgcaaatcg ccgtgagcag tatcaacaat caacttgttc agatcaaaca
     3841 actattgcct taaaatatcc tcgtaaatat gaggatgctc aagaaattgt ggatctttta
     3901 atagttaatg aatgcgtttt gattgatttt cagtttatgc tagatgctca ggctagacgg
     3961 tgtttagatt ttattgatgg tgctagtaaa gtgctctatg gtagcttaca aaaggtcggc
     4021 tcttcaatgt acttactggc tccgtcaaat gtatccgtca atatagaaga aatgactatc
     4081 ccacatacta cacaagatat tggctttgat tttgatatga aaaggcggta aataaatgat
     4141 attaatacta tctattcttc ttcgtctgat caaagtttac acttatttat tgattgctta
     4201 cgcattaatg tcatggtttc ctggggcata tgattcaaaa attgggcgtt tgattagtgg
     4261 tatcgttgaa ccaattttaa aaccttttag agcatttaat ttacaatttg caggtcttga
     4321 cttcactatt tttgtcgtca ttattagttt gaatttttta gctcaagttt tggtccgtgt
     4381 gtttatttaa tggttagtca tagtaagatt tatcagcatt ttcaccaaga agaatatcct
     4441 tttattgata gaatgtctga tatgattaat agagttgaag attactatct tttagaagtt
     4501 actgagtttt taaatcctag agaagtgatg attttaaaaa gtttgattgc tttaacagat
     4561 ctaaaaatgt tcgtatcaac agattactac ccaagcgaat atggtcgtgt cattattgca
     4621 cctggttact atgacttaga acaaagtgat tttcaaatag ctttagtaga gataagttat
     4681 caggcaaagt ttaatcagtt gacacatagt caaattttag gaactttaat taatgaatta
     4741 ggagtaaagc gaaatttatt tggagatgtt tttgttgaaa tgggatatgc ccagctcatg
     4801 attaagcggg agttattgga ttatttttta ggaacaatta ctaaaatagc taaaactagt
     4861 gtgaaattaa gagaagttaa ctttgatcag ttaattaggt ctattgataa cagccagacc
     4921 ctggatattc tagtttctag ttttcgatta gatggtgtag ttgctactat cttaaaaaaa
     4981 tctcgaacgc aagttatagc attaattgaa gcaaataaga ttaaggtaaa ctatcgactc
     5041 gctaataaag cttcagataa tctagtcata ggggatatgg tgagtatcag aggtcacggg
     5101 cgttttactc ttttagcaga taatggagtg accaaacatg gcaaacaaaa aataacacta
     5161 agtaaaatga tacataaata aggagataca atggcactta caacgctaga aattaaagat
     5221 aaaactttta agacaaaatt ccgaggatac tgtgaagaag aagtcaatga attccttgat
     5281 attgttgttg atgattacga agctcttgta cgtaaaaatc gtgataacga agcaagaatt
     5341 aaagatcttg aagaaaaatt atcttacttt gatgaaatga aagagtcact tagccagtct
     5401 gtaatcttag ctcaagaaac agctgaaaaa gtgaaagcaa cggctaatgc ggaagcaaca
     5461 aacttggtta gtaaagcgac ttatgatgct cagcatttat tagatgaatc taaagctaaa
     5521 gccaatcaaa tgttgcgtga tgcaactgat gaggctaagc gagttgcgat tgaaacagaa
     5581 gaactaaagc gtcaaacacg tgtgttccac caacgtttaa tctcatctat tgagtcacaa
     5641 ttaagcttat caaattcacc tgaatgggat gaactgctac aaccgactgc aatctatctt
     5701 caaaattctg acgatgcttt taaggaagtc gtgaagaccg ttcttaatga agacattcct
     5761 gaatctgatg atagtgcctc ttttgatgca acacgtcagt ttactccaga agagttagaa
     5821 gaattgcaac gtcgtgttga tgaaagcaat aaggagttag aggcttacca acttgactct
     5881 caatctgatt ctatgactga gccagaggta aatctcagtg aaacacaaac gtttaaatta
     5941 aatatctaaa taaagaaaaa caagatgatt tgcttataat cacagcaagc gggagatgat
     6001 gagagtctcg tattccctaa gtattatcat tatcattttc taaaattttc gctgaactac
     6061 agtaagcgag gaggtttgtt caccttacga gcaaagaggg agcggtaagt cttatgatat
     6121 attatcgctc tgaactaagg tggtaccacg agctttcgtc ctttttgaga cgaggctttt
     6181 ttcgttatag tcgcaagttt gtttaaaagg agtttgaaat aatgaaatta aaagagacac
     6241 ttaatctagg aaaaacagcc tttccaatgc gcgcaggtct tcctaataaa gagccacaat
     6301 ggcaagcagc ttgggaacaa gcagagcttt acaaaaaacg tcaagaatcg aatgcaggta
     6361 agcctgcctt tcatcttcat gatggacctc catacgctaa cggcaatatt cacgttgggc
     6421 atgcccttaa taaaatttca aaagatatca ttgtgcgttc taagtccatg tctggctttc
     6481 aagcgcctta tgtacctggt tgggacacac acggccttcc aatcgaacaa gtcttggcta
     6541 agcaaggaat caaacgtaag gagatggatt tagcagaata ccttgagatg tgtcgtcaat
     6601 acgctttaag tcaggttgat aaacagcgaa atgatttcaa acggttaggt gtttcggcag
     6661 actgggaaaa tccttatgtc actttggacc cacagtttga agctgatcaa attcgtgttt
     6721 ttggagcgat ggctgaaaaa ggttacatct atcgtggtgc taaacctgtt tattggtcat
     6781 ggtcttcaga atcggccttg gcagaagctg aaattgaata ccatgacatc gattcaacct
     6841 ctttatacta cgccaataaa gttaaagatg gtaaaggcat ccttgatact aatacgtata
     6901 tcgttgtttg gacaacaaca ccatttacgg ttacagcatc acgcggtttg actgttgggc
     6961 ctgatatgga ttatcttgtg gtgaaacctg ctggttcaga ccgtcaatat gttgtggcag
     7021 aaggtctttt ggatagcctt gctggaaaat ttggctggga atcttttgaa actttagcca
     7081 gccataaagg agctgattta gagtacattg tgacagaaca cccatgggat actgacgtag
     7141 aagaattggt tatccttggt gaccatgtta cccttgagtc agggacaggg attgtccaca
     7201 cagcaccagg ttttggtgag gatgactaca atgttgggac aaaatacaaa ctggaagttg
     7261 ctgtgactgt tgatgaacgt ggcctaatga tggaaaatgc aggtccagat ttccacggac
     7321 aattttataa caaggtaacg ccgattgtca ttgataaact tggtgatctt ttattggcac
     7381 aagaagtgat caatcactct tacccatttg actggcggac gaaaaaacca atcatctggc
     7441 gtgctgtacc gcaatggttt gcttctgttt ctgatttccg tcaagatatt ttggatgaaa
     7501 ttgaaaaaac aaccttccat ccatcatggg gtgaaacacg cctttacaat atgattcgtg
     7561 atcgtggcga ctgggttatt tctcgtcaac gtgcttgggg agttccgctt cctatcttct
     7621 atgccgaaga tggcacagct attatgacta aagaagtgac agatcatgtg gctgatttgt
     7681 tccaagaaaa tggatcaatc atttggtggc aaaaagaagc taaagaccta ttaccagagg
     7741 gcttcactca tccagggtca ccaaatggcg agtttacaaa agaaactgat atcatggacg
     7801 tttggtttga ctctggttct tcttggaatg gagtcatgaa tactaaagaa aatctttcct
     7861 atccagcgga tctctactta gaaggctctg accagtatcg tggttggttc aattcatcct
     7921 taattacatc tgtggctgtt aatgggcatg ctccatataa ggctatttta tcgcaaggct
     7981 ttgttcttga tggtaagggc gaaaaaatgt ctaaatcaaa aggcaatatc atttctccta
     8041 atgatgtggc taaacaatat ggtgcagata ttcttcgact ttgggtagct tctgtggata
     8101 ccgataatga tgtccgtgtt tctatggaga tccttggtca agtctctgaa acttaccgta
     8161 aaatccgtaa cacccttcgc ttcttgattg ctaatacatc tgatttcaac cctgctacgg
     8221 atacagtagc ttatgcagat ttaggagctg ttgataagta catgacaatt gtctttaatc
     8281 agttggtagc aacgattact gacgcttatg agcgttatga ctttatggct atttacaaag
     8341 cagtggtgaa ctttgttacg gttgatttat cagccttcta tcttgatttt gctaaagacg
     8401 ttgtctatat tgaagctgcc aatagcttag aacgtcgtcg catgcagaca gtcttctatg
     8461 atatcttggt taagattacc aagttattga cgccaatctt gcctcacact actgaagaaa
     8521 tctggtctta tctagagcat gagtcagaag catttgttca attggcagaa atgcctgtgg
     8581 cagaaacttt ctcagctcaa gaggatattt tagaagcttg gtctgctttc atgactttgc
     8641 gtacccaagc tcaaaaagct ttggaagaag cgcgaaatgc taagattatc ggtaaatcat
     8701 tggaagccca tttgaccatc tacgctagcg aagaagtgaa aaccttattg actgctttag
     8761 acagcgatat tgctttgctt ttgatcgtgt ctcaattaac cattgctgac ttggcagatg
     8821 cgcctgcgga tgcagtggca tttgaaggta ttgcctttat ggtagaacat gccataggtg
     8881 aagtttgtga gcgttcacgc cgcatcgacc caactactcg catgcgttct tacaatgcat
     8941 ttgtctgtga tcacagcgct aaaatcattg aagaaaattt cccagaagct gtggctgaag
     9001 ggtttgaaga gagtggcaaa taagtcttat caagacttga agtgacactg agtaagaaga
     9061 caaccatcgg gtcttaggac ctgatggttt tttagtagag ctgattacgt ccattagcca
     9121 gctttaatga gagacaaagt aggagaaatt tagactactt tttttatttc ttttaagaaa
     9181 attgctggct tcatataagg actattaaga tagagttcac ctttcgctaa taaaaaaact
     9241 tagctctttt agaagggcta agttttgtct gaatagctag ctgctgttta tacagaagtt
     9301 tgaaatgaaa tttcaataag gttattcgtg tgaagtgttt ttgattgtcc aggaataaca
     9361 attttaggat caattttacg ttttaaaaaa aattcgcgaa tgatatcaag ttcatcctcc
     9421 ttgatggcac ggtatttatt tgaaataagt agttcgtaat gttttggtgc ttgggtaaaa
     9481 atgacatctg tatttcctgc tgagtatact tcgactaaat aggcatcggt gttttttagc
     9541 tgatttctga tgagttctgg gtgactgcta gtggtattga tgagtctcat aaatgtcctc
     9601 cttagtattc ctgtacctat tatagcacta atttaaagcc ttagttagta gaatgatgct
     9661 ttttttgcca ttccttgact ccccattgat ggctctctcg ttttaattta gcaattgcct
     9721 cgataggaga aaaccaacct aagttattaa aatcttctaa gggcttagaa acggcttgaa
     9781 atgcagttac ttcataaaga taggcaggat ggtagaagtg ggtatcacga tggcgagagt
     9841 agaaatattc atctgcctga ccgtaatatg aaccaatagt agcagaaaaa ccaagttctt
     9901 caatcagttc acgctctagg gcctgaagct gaccttcacc tgcttcaatt tcaccaccag
     9961 gtaaaaacca ggaaccatta ggagcttgaa caagaataat ttttgtttgt tcgtgattgg
    10021 gaatgatagc atagacaccg taacgtgtga catagtcttt atgtgcattt ttatgtccaa
    10081 atgttggaat catcatttta tttacctttc ctttttcttc attatactaa atttttgcta
    10141 attattctta tcaatttgtt tagtttaata aaaaattgat agaaaaagag agagtctcct
    10201 tatagaaact ctcttagcta acgttaatcg agagactctt cgttaagaaa agaatctccc
    10261 tctttaacag ctttaatcgt gatgttttca ctgtttgtca tcagtgcacg taactgtttt
    10321 tcagttgggt gttctaaata atagtctgta atggcatctt cgatataatc ttggatagta
    10381 cgacggagcg gtcgagcgcc cattttagga tcatagccta aatctactaa tttttctttg
    10441 accttttgtg tcacatcaag atgaatccca ttatagccta agcgctcatt aacatcttcc
    10501 agcattaaat caacgatatg cagcaaatgc tctttagata aagctttgaa ttcaataata
    10561 ccatcgaaac gattcataaa ctctgggctg aagaagttgc ttaattcacc aagaacagaa
    10621 cttgtccgtc cttctctagc agcaccaaat ccgacagaag cttcgctttt acctgttccg
    10681 gcattagagg tcatgataat aatggtgtcc ttgaagctga ctgttcgtcc ttgaccatct
    10741 gttaaacggc catcatcaag aacttgtaag aacatgtgca tgacgtcagg atgggctttt
    10801 tccacctcat ctaagagaat aagtgaatat ggattgcgac gaacttgttc ggttagctgt
    10861 ccagcttctt catagccgac ataacctgga ggagccccga ctaatttggc gacagcgtgt
    10921 ttttccatgt attcggacat gtcaaagcga atcatattgt ttgtcgaacc aaagagttca
    10981 atggctagtt gtttagacaa ttcagtttta ccaaccccag tcggtccaac gaataagaaa
    11041 gaaccaatag ggcgatttgg agttcctaat cccacacggt tacgacgaat agccttagcg
    11101 attttatcaa cagcatcgtc ttgcccgatc acgtgtgctt tcagatcatt ggctaagttt
    11161 acgagttgag actgttcctt ttcttttaag tccccaactg gaatattagt tttttgctct
    11221 acaatagctt caatggtttt ttcagtaatg atgggaatat cttgctcatc gactttttga
    11281 gcctgcattt ctttgtattt tgtaatttga tcgcggaaat aagctgcgcg ttcatagtct
    11341 tcgtctcgag tagcttgcgc cttgagattc tcagcttcaa taagacgttt gtcaatttct
    11401 ttaggatcaa caaagttgag ggttagattc attttggaac cagcttcgtc cagaagatca
    11461 atagccttat caggaagaaa acggtcttga atatagcggt tagataaatg agcggcagct
    11521 tcaatagcgg ctgggctata tttgacatga tgatagtctt cgtatttcgg ttggatacct
    11581 tttaaaatcg tgatggtttc ctcaacagaa ggttcatcaa ctttaacggg ttgcatacgt
    11641 cgctccaagg cagcatcttt ttcaataata cggtattcat ttaaggtagt agcaccaacg
    11701 agttgcaact caccacgggc caaggctggt tttaaaatat taccagcatc catattgccg
    11761 tctcctgcag aaccagcacc gacaatctca tgaatttcat caataaagag aatcacatcc
    11821 ttgcgattgc gaatttcttc cattaatttt tgcatgcgct cttcaaactg accacggata
    11881 cctgttccct gaacgaggct aaccacatca agacgaatca cttgcttgcc ttggagtttt
    11941 tgaggaacag taccatcaat aattttttga gccaaacctt ctacaacagc agttttacca
    12001 actccaggct caccaattag cacaggatta tttttagtac ggcggttgag gatctcgata
    12061 acgcgtgtaa tctcttcgtc acgaccaata acaggatcaa tattaccatt tcttgcaata
    12121 tctgtgacat taatcccaaa ctcttctaat aagccctttg cttgctgatt tggtgtttgt
    12181 ggctgagcag gtcgttgtcc gttgtagtta ccaccatagc gtcctccgcc atttccattt
    12241 tgccctgcct gagtaggtgg ggtatttgga agattaccaa aagctctaaa attattcaaa
    12301 tcaccaaaaa agtcatcaaa gaaaggactt gtggatctat cttgtgctct atatcctggg
    12361 gttaggccat ttaaaataga attagtagga tcggatttca tgatttgata acaattttga
    12421 cagagatcaa cctgtctttg ttttccatta acacttgtat aaagatgaat agtagattcg
    12481 tttaaattac aattttgaca cagcataata tacctccgag ggtttatcta aaagtcaaag
    12541 tcatttagtc aaaaatggtc aaactttata ttcatattat agcaccacgg cttgggaaat
    12601 caagtaaaaa gtttatcgct ttagcgttag cttttagaga gtaacatgac agagtaactt
    12661 tattaagttt ccaatgagca aatatttaaa atcattgaaa agagtaaaaa aaagtaaaat
    12721 atcaataaat atattacttt ccttgttagt ttcccttgtt ttgtgttaaa ataagaggga
    12781 taaaaaacga ataggagaaa cgaaatggaa tcacatttgg tgagaatcat caatcgtttg
    12841 gagttgatgg tgacagatgg cggtaatctc aagcgaaatt ttgaacgcga tggtgttgtt
    12901 gttgcagaag ttgccttttc aaatgatcct gaaaatggac ctgttttcac attgcgtgat
    12961 gttgaagcgc gtgagtcata ttcctttgat agcattgact tgattgcaat ggaaatctac
    13021 gatttattat attaagcttg acatagtaaa gagacttgcc tgaggcaggt ttttttattg
    13081 tcaaaaagta ttgtattaat attcatcaga tggtataatt atctttattg taatgaacct
    13141 aggacttatg aggagatata aatggatttg tcatttttgc ccaaatactg ggcctacttt
    13201 aactacggtg tacttgtcac cattatgatt tcaatcagcg ttgtcttttt tggaaccctt
    13261 attggtgtct tggtaaccct gattaagcgt agtcatgtga agccgttgac ctgggtcgtt
    13321 aatctttacg tttggatctt tcggggaaca cctatggtgg ttcaaatcat gattgccttt
    13381 gcttggatgc attttaacaa tatgcctact attggttttg gggttttaga cttggacttt
    13441 tcaagactac ttcctggaat tattatcatt tcattgaata gcggtgctta tatttcagaa
    13501 attgttagag caggtattga ggctgtacca aaagggcaat tagaagcagc ttattcacta
    13561 ggtattcgtc ctcaaaatgc catgcgttat gtgattttgc ctcaggcctt taaaaatatt
    13621 ttgccagcct taggaaatga atttattact attattaagg atagtgctct tttacaaacc
    13681 attggagtga tggagctttg gaatggtgcc caatcagtgg taacggctac ttattctcca
    13741 atttcccctt tactggtggc tgctttttac tacttaatga tcacaacagt gatggcacag
    13801 ttattggcag tcttagaacg tcacatggcg caaggaggta atcattgatg actgaggcta
    13861 ttatttcaat taaagatttg cacaagtatt atgggcacaa tgaagtgctt aagggaattg
    13921 atttagacat tatgccagga gaggttgtgg tgattattgg cccttctggt tctggaaaat
    13981 cgaccttact tcgaactatg aatcttttgg aagtaccaac taagggacag attaggtttg
    14041 aggggattga tattaccgat aagaagaatg atattttcag catgcgtgaa aaaatgggaa
    14101 tggttttcca gcagtttaac ctctttccca atatgactat tttagaaaat atcactttat
    14161 cgccaatcaa aactaaggga atggctaaag cagaggctga caaaacagcc ttgagcttgt
    14221 tggacaaagt tggattatca gaaaaagcca aggcttatcc tgctagtctt tctggtgggc
    14281 aacagcagcg gattgcgatt gcgcgtggac tggctatgga tccagatgtt ttactctttg
    14341 atgaaccaac ttcagctcta gacccagaaa tggtgggtga ggtcttggct gtcatgcaag
    14401 atttggctaa atctgggatg actatggtta ttgtgactca tgaaatgggc tttgctaaag
    14461 aagtcgcaga tcgtgtgatt tttatggatg gcggcgttat tgtggaagaa ggaagtccta
    14521 accaactttt tgatctgact aaagaagaac gtacaaaaga ttttttgagt agagtattat
    14581 aaatctgttc tatgaat
//
LOCUS       DATURV010000040        14562 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DATURV010000040 DATURV010000000
VERSION     DATURV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.040"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            25..291
                     /locus_tag="VCF75_001168"
     CDS             25..291
                     /locus_tag="VCF75_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP2546732.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSTP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            325..1059
                     /locus_tag="VCF75_001169"
     CDS             325..1059
                     /locus_tag="VCF75_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546733.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            1064..2692
                     /locus_tag="VCF75_001170"
     CDS             1064..2692
                     /locus_tag="VCF75_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546734.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQEKHPKENFKAYKSMM
                     RQQALMIAIFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGTVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVVQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKVKQTKKPVNRPHQNQSLAQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     EFMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISVLLSCLTLMVLGLAQLWIQKTFWKSLERL"
     gene            2757..3578
                     /gene="proB"
                     /locus_tag="VCF75_001171"
     CDS             2757..3578
                     /gene="proB"
                     /locus_tag="VCF75_001171"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEP2546735.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKSPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            3571..4821
                     /locus_tag="VCF75_001172"
     CDS             3571..4821
                     /locus_tag="VCF75_001172"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEP2546736.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNCFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAMAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDAFHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            4841..5056
                     /locus_tag="VCF75_001173"
     CDS             4841..5056
                     /locus_tag="VCF75_001173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546737.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFVFLEMVC
                     WSAGSNVVSKFNQLLIDLQVTLQNCRR"
     gene            5099..5263
                     /locus_tag="VCF75_001174"
     CDS             5099..5263
                     /locus_tag="VCF75_001174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546738.1"
                     /translation="MSWDKIFDIWLDNLLTDCCVIKEAKNLLQAVETLEAAAKSVLAK
                     LADSEQKEQD"
     gene            5287..6300
                     /gene="rsmH"
                     /locus_tag="VCF75_001175"
     CDS             5287..6300
                     /gene="rsmH"
                     /locus_tag="VCF75_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEP2546739.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLQSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPTFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            6305..6628
                     /gene="ftsL"
                     /locus_tag="VCF75_001176"
     CDS             6305..6628
                     /gene="ftsL"
                     /locus_tag="VCF75_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEP2546740.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            6632..8887
                     /locus_tag="VCF75_001177"
     CDS             6632..8887
                     /locus_tag="VCF75_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP2546741.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGTGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            8889..9899
                     /locus_tag="VCF75_001178"
     CDS             8889..9899
                     /locus_tag="VCF75_001178"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEP2546742.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLAGGLMFYFLHVSPSGISSINVFGYQLSLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGTMIGALLGFFL
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            10057..11400
                     /locus_tag="VCF75_001179"
     CDS             10057..11400
                     /locus_tag="VCF75_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2546743.1"
                     /translation="MSFKDYHFKQYIQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDETKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            11595..12398
                     /locus_tag="VCF75_001180"
     CDS             11595..12398
                     /locus_tag="VCF75_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP2546744.1"
                     /translation="MTSVFLTSGWAFYDHLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKCLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            12398..13141
                     /locus_tag="VCF75_001181"
     CDS             12398..13141
                     /locus_tag="VCF75_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2546745.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            13245..13469
                     /locus_tag="VCF75_001182"
     CDS             13245..13469
                     /locus_tag="VCF75_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEP2546746.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            13533..14450
                     /gene="trxB"
                     /locus_tag="VCF75_001183"
     CDS             13533..14450
                     /gene="trxB"
                     /locus_tag="VCF75_001183"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEP2546747.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDSEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
BASE COUNT         4499 a         2547 c         3092 g         4424 t
ORIGIN      
        1 agtacaaaaa ataaaggagt ataaatggaa aagaaacgac agcgcttata cgatgtgatt
       61 agacaggctt atgattatcc agaaaaccgt gaaaatgtgg ccttgtcgca gctactttta
      121 gcagcttcta atagattaat taaacacagt accccacttc ttgttgccta ccaactaaac
      181 caagatgtgg ataattactt acttgataat gacattttac ttccaaaatc gctttgccgt
      241 tttaagcagt cattggaaaa gtatatttta gaggaagcct catcagtcta gggagaaaaa
      301 gagaagaaaa ggaaaaagga taccatgata gaatttaaac atgtttcaaa gttgtatggc
      361 gacaaagaag ctctcagcga tttaaacgtc accatcaatg acggtgaaat ttttggcctt
      421 atcggacata atggtgcagg taaaaccacg accatcagca ttttgacttc tattatcgaa
      481 gcgagttatg gcgaagtatt tgtggatggt caacttctga cagaaaatag agaagctatc
      541 aaaaaacaaa ttgcttatgt gccagattct cctgatattt tcttgaatct aactccaaat
      601 gagtattggc aatttttagc caaaatttat ggggtgtcag atgaggaccg agaagaacgg
      661 ttagcacagc tgacaacact ttttgaactg aaggaagaag ttaatcaaac cattgatagt
      721 ttctcacatg ggatgcgcca aaaagtgatt gtgattggtg ctcttgtatc aaatcctaac
      781 atctggattt tggatgaacc attaacaggt ttggatcctc aggcttcttt tgaccttaaa
      841 gaaatgatga aagctcatgc agcttctgga catacggtct tattttcgac tcacgtgcta
      901 tcagtcgctg agcagttatg tgatcgtatc gggattctaa aaaaagggaa attgattttt
      961 gttggaacga ttgatgagtt gaaagaacac catcctgaca aagacttgga gagcatctac
     1021 cttgaactcg ctgggcgtaa ggcgcaagaa gaggggtgat gggatgaatt ggtctactat
     1081 ttgggaactt atcaagataa acattcttta ttccaatcca caaagtttag ctaacctcaa
     1141 aaagcgtcag gaaaaacacc cgaaagaaaa cttcaaggcc tataaaagca tgatgaggca
     1201 acaggcctta atgattgcca tatttttggt gatttacctt tttatgttta ttggtgttga
     1261 tttcagtcat tatccaggac ttttctcctt tgacgttgct atgtttttta tcatgtcaac
     1321 cttgacagcc tttagctctc tttacaccat tttttatgaa agtaatgacc taaaacttta
     1381 tattcactta ccagtaacgt cagaagaact ctatattgca aaaattgtct catcgctagg
     1441 gatgggtact gtctttttga tgccccttat ctctcttctc ttaattgcct attggcaact
     1501 cttgggaaat cctttgtcta ttttagtagc tatcgtgctc tttttagtgt tactagtgag
     1561 ttctatggta ttagctattt atatcaatgc ttgggtaggc aaaatcattg taagaagtcg
     1621 caaacgaaaa ctcatttcaa ctatcatgat gtttgtctca acttttggcg ctttcgtctt
     1681 aatttttgcg attaatatta gtaataacaa gcgtacgatg acggatggcg tatttactga
     1741 ttacccaact attccctatt tcaaaggatt ctatgatgtt gttcaggcac cgttttctac
     1801 tgcggccctc cttaactttt ggttgccatt gttacttatc ctagctatgg tatatggtat
     1861 tgtcacaaaa gtgatgccaa cttattatcg tgaggctttt tatattagta acgagaacaa
     1921 agtcaagcaa actaaaaaac cagttaatcg tcctcatcag aatcaatcac tggcgcagtt
     1981 gttgcgaaaa catcacctat taacgttaca aaatgcaact ttactgacac aaacctatct
     2041 catgcccctg atgtatgtga tgctttttat cggtccaagt ttgtcacgtg gcacaggttt
     2101 ctttaagcat atttccccag attactttgg ggtggcctta ttatttgggg tcagtttggg
     2161 tgtcatgtgt gcaacaccaa ccagctttat tggagtaggt atttcacttg aaaaggataa
     2221 ctttaccttt attaaaagct taccaataac gttaaaaaaa ttcttgctgg ataaattttg
     2281 cttgcttgta ggtctgcaac tgattgtgcc tatggtgatt tatcttgtgt ttggtctctt
     2341 tgtgttgcac ctgcatcctc ttctaacgat tgctttttgt cttggctacg ccctgtctct
     2401 gattgtgcaa ggggagttta tgtatcgtcg tgactaccgg cttctggatt taaaatggca
     2461 agacatgaca caactcttta ccagaggaaa tgggcagtgg ctaacaatgg gccttatctt
     2521 tggtaattta atagtcacag gtgtagtagg ttttggagct gttatcattg ccaatattat
     2581 ccaacaacct ctgttgatta gtgttctact gagttgtctg acactaatgg ttttaggtct
     2641 tgcgcaatta tggattcaaa aaaccttctg gaaaagctta gagaggcttt aacgaagagg
     2701 gatcattatt cggaaaaaca gtcctctggg gctgtttttt atgctataat ttgggtatga
     2761 tgaaacgaca atttgaagat gtaacacgta tcgtaattaa aattggcact agttcattag
     2821 tgctaccaac aggtaaaatc aatttagaaa aaatcgacca actggctttt gtgatttcaa
     2881 gtttgatgaa taagggcaag gaagttattt tagtatcatc gggagcgatg ggatttggac
     2941 tagatatttt gaaaatggaa aaacgtccga ccaatcttgc taaacaacag gcagtttcca
     3001 gtgttggtca ggtggccatg atgagtcttt attcacaaat ttttgcacac taccaaacca
     3061 acgtgtcgca aattttattg acgagagatg ttgtggtttt tccagaaagt ctagccaatg
     3121 ttaccaatgc ttttgaaagt ctgattagtt tgggcattgt gccgattgtt aatgaaaacg
     3181 atgcggttag tgtggatgaa atggatcatg cgactaaatt tggagataat gaccgtttat
     3241 ctgctgtcgt tgctggtatt acaaaagctg atttattaat catgttgtct gatattgatg
     3301 gcctttttga taaaagccct actatttatg aggatgcgca gctacgcagc catgttgcgg
     3361 tcattacgca agaaatcatt gcgtcagcag gtggtgcggg cagtaagttt ggcacaggtg
     3421 ggatgcttag caaaattcag tctgctcaga tggtatttga aaacaaagga cagatggtct
     3481 tgatgaatgg agctaatccc agagatattt taagagtgct tgaagggcaa ccactaggca
     3541 cctggttcaa gcagatagaa gaggtaacac atgactgata tgagaaggtt agggcaaagg
     3601 gcaaaacaag ctagtctttt aatagcacct ttgtcgacgc aaattaaaaa ttgcttttta
     3661 agcaccttag caaaggcatt agttgacgat acacagactc tcttagccgc taaccaaaaa
     3721 gatttagcga atgctaaaga acatggtatt tctgacatca tgatggatcg cttgcgctta
     3781 acttcagaac ggattaaagc gatggcacag ggagttcaac aggttgctga cttagctgat
     3841 ccaatcggac aagtcattaa gggctatact aacctcgatg gccttaaaat cctccaaaag
     3901 agagttcctc taggggtgat tgcgatgatt tttgagagtc gcccgaatgt ttcagtggat
     3961 gcttttagtc tggcgttcaa aaccaataat gccatcatcc taaggggagg aaaagatgcc
     4021 tttcactcaa ataaagcttt ggtgaagttg atccgacaaa gtctggaaaa atcagggatc
     4081 accccagatg ctgtccagct agttgaggat ccctcacatg ctgtcgctga agaattgatg
     4141 caggcaacgg attatgtaga tgttttgatt ccacgtggtg gtgctaaatt aattcagacc
     4201 gttaaggaaa aagcaaaagt gcctgtcatc gaaacaggtg tggggaatgt gcatatctat
     4261 gtggatgctc aagctgactt agatatggct actaagattg ttatcaacgc taaaacaaag
     4321 cgcccaagtg tttgcaatgc agctgaggga ttggtaattc acgaagctgt agcagcaagg
     4381 tttattccta tgcttgaaaa agcgatcaat caggttcagc cagtagaatg gcgggcagat
     4441 gacaaggctt tgccgttatt tgagcaggca gtgcctgcaa aagcagagga ttttgagacc
     4501 gaatttttag attatatcat gtctgttaag gttgtttcct ctttggaaga agctatttct
     4561 tggataaatc agcatactag tcaccattcc gaagctatca tcacaaggga tattaaggcg
     4621 gcagagacct ttcaagattt agtggatgct gctgctgttt atgtcaatgc ttcaacacga
     4681 tttaccgatg gttttgtttt tgggcttggt gctgaaattg ggatttctac tcaaaaaatg
     4741 cacgcccgtg ggccaatggg cttagaagcc ttgacaagca ctaaatttta tattaatggg
     4801 gatggacata tcagagaata agctactaga aggtccctta atgactaatg aaagagaaaa
     4861 agaagaagca aaggttgctt tagaagaact tttacatcag attcaggtga ttcatttgga
     4921 attgaaaatt gtccgaaatt ggggctattt tgtttttttg gagatggtat gctggtcagc
     4981 tggatcaaac gtcgtgagca agtttaacca attattaata gacttacaag ttactcttca
     5041 aaactgccga agatagctgg cagacgtcga taggctatta ttagctgact taccgaatat
     5101 gagttgggat aaaatctttg atatttggtt agacaacctg ctgacagatt gttgtgtgat
     5161 taaggaagct aaaaatcttt tacaagcagt tgaaactcta gaggcagcgg ctaagtcagt
     5221 gcttgctaag ttggctgata gcgaacaaaa agaacaagat tagagaagag gaagattccc
     5281 acgagaatga aatccttttc tcttactttt tcatttctaa accttttgaa gtatggtaca
     5341 attaaagtta tgacaaaaga atttcatcac gtgaccgtac tccttcacga aacagtggac
     5401 atgcttgaca taaagcctga tgggatttat gttgatgcga cgctaggtgg ctcagggcac
     5461 tcagcttatt tgttgtccaa acttggtgaa gaagggcacc tctattgttt tgaccaagac
     5521 caaaaggcta ttgacaatgc acaagttacc ctccaatctt atattgacaa aggacaggta
     5581 acttttatta aagataattt tagacacctc aaagcacgtt taacagcgct tggagttgat
     5641 gaaattgatg gtatcttata cgaccttggt gtttccagcc cgcaattgga tgaaagagaa
     5701 cgagggtttt cttataaaca agatgctcca ttggatatgc gcatggatcg tcagtcgctc
     5761 ttaacagctt acgaagtggt gaatacctat ccattcaatg atttggttaa gatttttttc
     5821 aaatatggtg aagataaatt ctccaagcag atcgctcgaa aaattgaaca agcaagagct
     5881 attaagccta ttgagacaac aacagagttg gcagaattga ttaaggcagc aaagccagct
     5941 aaagagttga agaaaaaagg ccatcctgct aaacagattt ttcaagctat tcgcattgaa
     6001 gtcaatgatg aattgggagc ggctgatgaa tctattcagg acgctatgga attattagcc
     6061 cttgatggtc gtatctcagt cattaccttc cattctctgg aagatcgcct aaccaagcag
     6121 ttgtttaaag aagctagtac ggtggatgtg ccaaaagggc ttcctctaat tcctgaagat
     6181 atgaaaccga catttgaact tgtttcacgt aagccgatct tacctagtca ttcagagtta
     6241 acagctaata aaagggcaca ctcagccaag ctacgtgttg ccaaaaaaat tcggaaataa
     6301 gcctatgaca aatgaaaaaa gaactcaggc agttacaaat gccttgcaaa aacgaataaa
     6361 aactttctct agaattgaga aggcttttta tactgctatc attgtaacag cgattacgat
     6421 ggcagtaagt attatttacc tgcaaagtcg taagttgcag ctacagcaag aaatcactag
     6481 tttaaatagt catatttcgg atcaaaaact agagcttaat aatgccaagc aagaagtaaa
     6541 tgagttatct cgccgtgatc gtattataga tattgctggt aaagcaggct taagcaaccg
     6601 taataataat atcaagaaag tcgagtaatc tatgaaaaaa tggcaaaaat atgttttaga
     6661 ttatgttgtg cgcgatagga gaactccagt cgaaaatcgc gttcgagttg gacaaaatat
     6721 gatgctctta actatcttta ttttctttat tttcattatt aatttcatga ttattattgg
     6781 aacagatcaa aagtttggag ttagtttgtc agaaggggcg aagaaggttt atcaagaaac
     6841 cgttacgatc caagctaagc gtgggaccat ttatgatcga aatggtacag ctattgcagt
     6901 ggattctacg acttatagca tatacgcaat tttggataaa tcatttgtct cggcttcaga
     6961 tgaaaagtta tatgtacaac cttctcagta tgaaacagta gctgatattt taaaaaagca
     7021 tctgggaatg aaaaaaacag atgtaattaa acagcttaag cgtaaaggac ttttccaagt
     7081 ctcgtttgga ccgtcagggt ctggcatttc atatagtact atgtctacta ttcaaaaggc
     7141 tatggaagat gccaaaatca agggaattgc tttcacaact agtcctggtc gtatgtatcc
     7201 aaatgggaca tttgcttcag aatttatagg cttagcatct ctaacagaag ataaaaagac
     7261 aggtgttaag agtttagttg gaaaaacagg tctagaagct tcttttgata aaattttatc
     7321 aggtcaagat ggtgttatta cttatcaaaa agatcgaaat gggaccacac tcttgggtac
     7381 aggtaagact gtcaagaaag ctattgatgg caaagatatt tacacaacgc tatctgagcc
     7441 tatccagacc ttcttagaaa cccagatgga tgtttttcaa gccaaatcaa atggtcagtt
     7501 ggccagtgca acacttgtta atgctaaaac tggtgaaatt ttggcaacaa cacaacgccc
     7561 cacttataat gctgatactc tgaaaggact tgaaaatacg aactacaaat ggtacagtgc
     7621 acttcatcaa ggaaattttg agccaggctc aaccatgaaa gtgatgactc tggcagcggc
     7681 tattgatgat aaagttttca acccaaacga aacctttagc aatgctaatg gtttaacaat
     7741 tgcagatgct actattcaag actggtctat taacgaaggc atttccacag gacagtacat
     7801 gaattatgca caagggtttg ccttctcaag taacgttggg atgactaaac ttgaacaaaa
     7861 aatgggtaat gcaaaatgga tgaattattt gacgaagttc cgctttggtt ttcctactcg
     7921 ttttggttta aaagatgaag acgcaggtat atttccttct gataatatcg tgactcaagc
     7981 tatgagcgct tttggtcaag ggatttctgt aacccagatt caaatgctta gagcttttac
     8041 tgctatttct aataatggtg agatgttaga gccacaattt atcagtcaaa tttatgatcc
     8101 taacacagca agttttagaa cggcaaataa agaaattgtt ggaaaacctg tatcaaaaaa
     8161 agccgctagt gaaacaagac aatacatgat tggtgtagga acagaccctg agtttggaac
     8221 actctattca aaaacatttg gaccaattat taaagtgggt gatttacctg ttgctgttaa
     8281 atcaggaaca gcacaaattg gttcagaaga tggaactggt tatcaagatg gtggattgac
     8341 taactatgtt tattcggttg tggcaatggt gccagctgat aaaccagact ttttgatgta
     8401 tgttactatg actaaaccac aacattttgg tccccttttt tggcaagatg tggttaaccc
     8461 agtattggaa gaagcatact taatgcaaga tacactaact aagccagtag tatcagatgc
     8521 taatcgtcaa acaacttata aattaccaaa ctttgtagga aagaatcctg gtgagacatc
     8581 aagcgagttg cgtcgaaatc ttgtccagcc agttgtcctt ggtactggca gcaagatcaa
     8641 aaaagtatcg catcagcccg gtcaaacgtt aacagaaaac caacaagttc tcatattatc
     8701 agaccgtttt gtggaggtac cagacatgta tggctggaca aaatccaatg ttaaaacctt
     8761 tgctaaatgg actgggatag acatcagctt taaaggaaca gattctggtc gtgttatgaa
     8821 acaaagtgtt gatgttggta agtccttgaa aaaaataaaa aaaatgacca ttactttagg
     8881 agattaacat gtttttaacc cttattgcag caatcatatc ctttatggta tctgctttca
     8941 caatgcctta ttttatcaaa ttctatcaat tgaaaaaaat tggtgggcaa caaatgcatg
     9001 aagatgtcaa gcaacattta gctaaagctg gaacaccgac tatgggggga actgtttttc
     9061 ttttagtagc tacggcagta tcgttattgg ttagtctatt ttcgatcaaa aacactcaaa
     9121 gtttagcttt gatttcaggc attttatcaa ttgttgtcat ttacgggatt atcggatttt
     9181 tggatgattt tttaaaaatt tttaagcaaa tcaatgaagg attgacagcc aaacaaaaat
     9241 tagccttgca gttggctgga ggattgatgt tctatttttt acatgttagt ccaagtggca
     9301 tttcttcgat caatgtgttt ggttatcagc tgtcgctggg aattttctac ctattctttg
     9361 ttctattctg ggtggttggt ttctcaaatg ccgttaattt gacggatggt atcgatggct
     9421 tagcttctat ctcagtggtg attagtttag tgacttacgg cgttattgcc tacgttcaga
     9481 gtcaatttga tgttttgtta ctgattggaa ccatgattgg tgctttactt ggtttcttcc
     9541 tatttaacca caagcctgcc aaagtattta tgggagatgt aggtagttta gcccttggag
     9601 ctatgttggc agctatttct attgcgcttc gtcaagaatg gaccttactc attatcggaa
     9661 ttgtctacgt tcttgaaaca agttctgtga tgttgcaagt gtcctatttc aagtacacca
     9721 agaaaaaata tggagaaggt cgtcgtattt ttagaatgac gcctttccac caccatttgg
     9781 aattgggagg cttgtctggt aaaggcaaga agtggtcaga atggcaggta gatgcttttt
     9841 tgtggggagt aggaagtcta gctagtttac tagttttagc tatcttatac gtcttctaag
     9901 ggggattatc aagaatagtc accaatagca ttttgattaa catcatcaat aagagagtgg
     9961 agatgaccca caaatactga ttttgtgagt gttgtcccac tccttttgct tgtctctaac
    10021 aataatgata taatatggaa gaaaacgagg taaaacatgt catttaaaga ttatcatttt
    10081 aaacaataca tccagcaagc actggaagaa attggttttg tcaatccaac agaggtccag
    10141 aagcggctga ttcctattgt taactccggt cgtgacttag ttggcgaatc aaaaacaggt
    10201 tcagggaaaa cccacacctt tttgttgccc atttttgaaa aacttgatga aactaaagca
    10261 gaagtgcaag tcgttattac agcaccaagc cgtgaattag caactcaaat ttttgatgct
    10321 tgtaaacaga ttgctaagca cttccaagaa gagattcgtt tggccaacta tgttggaggc
    10381 acagacaaac tccgtcagat tgaaaaacta aaaggcagcc agccgcatat tgtcatcgga
    10441 accccaggtc gtatttatga cttggtcaag tctggtgatt tagcgattca taaagcaacg
    10501 acctttgttg tggatgaggc cgatatgacc atggacatgg gctttttgga tacggtagat
    10561 aagattgcag cttcgcttcc aaaatctgtt cagattttgg tgttctcagc aactattcca
    10621 cagaaattac aacccttttt gaaaaaatac ttgaccaatc ctgttattga acaaatcaag
    10681 acaaagaccg ttatcgcaga taccattgat aactggcttg tgtcgacaaa agggcgtgat
    10741 aagaatggtc aactgttaga gattctcaaa accatgcagc cttatatggc tatgcttttt
    10801 gtcaatacca aggaacgcgc agatgatttg catgcctttt taacagctaa tggcttaaaa
    10861 gtggctaaga ttcatggggg cattcctcca agagaacgta agcgcatcat gaaccaagtc
    10921 aaaaaactgg actttgaata cattgttgcc acagacttag cagcgcgtgg gattgacatt
    10981 gaaggggtca gccatgtgat caatgacgcc atcccacaag acttgtcttt ctttgtccat
    11041 cgtgtaggcc gaacaggtcg aaatggtatg gcaggaactg ccattacact ttaccagcca
    11101 agtgacgact cagatatcaa agagctggaa aaaatgggga ttgcctttac accgaaggtg
    11161 ctcaaaaatg gagaatttca agatacctat gaccgtgatc gccgtcaaaa ccgtgaaaaa
    11221 gcctatcaaa aattagacac agaaatgatt ggtttggtga agaagaaaaa gaaaaaagtc
    11281 aaaccaggtt acaagaaaaa aattcagtgg gcggttgatg aaaaacgtcg caaagaacgt
    11341 cgtgctgaaa atcgtgctaa gggccgcgct gagcgtaagg ccaaaaaaca acatttttaa
    11401 gaacaaagag caagcaagtg atcaattggc atctaatctt gcttttttta cacctgctac
    11461 ttgtattgtt taccatttgt taacacaaaa agtgatcata gcttttgctt attgctataa
    11521 tacggattta ctattttaca tgtgggtcta agagtgttag aatattaaga caaacaaata
    11581 caaaggggaa atacatgaca tcagtgtttt taaccagtgg ttgggctttt tatgatcacc
    11641 tcatctcacc gattccacat gggaaacttt ttagttggca tgctgttttt gatgccattc
    11701 caaatattat ccaacggctt ccaattacgc ttggtttgac actatcagga gcaacctttg
    11761 gcttggtttt ggctttgatt tttgcccttg tcaaaattaa taaagtgaag ctactatacc
    11821 ctattcaagc tatttttgtg agtttcttgc gaggaactcc tattttggta cagttgatgt
    11881 tgacctacta cggtattccc ctctttctaa aatgtctcaa tcagaagtat ggctttgatt
    11941 ggaatgttaa tgcaattccg gcttctattt ttgccattac agcctttgct tttaatgaag
    12001 cagcctacgc tagtgaaact attcgggcag ccattttatc agtagataca ggtgaaattg
    12061 aagcagcaaa aagtctaggc atgacttctg tgcaggttta ccgtcgtgtc attattccta
    12121 atgcaactgt tgtggctatt ccaaccttga ttaatgggtt aattggctta actaaaggaa
    12181 catcgcttgc ctttaatgca gggattgtcg aaatgtttgc tcaggctcaa attctaggcg
    12241 gatcagatta ccgttatttt gaacgttata tctctgttgc tcttgtctat tggtctatca
    12301 gtattttgat ggagcaagta ggtcgcttga ttgaaaacaa gatggccatc aaagcaccag
    12361 aacaagctag aaatgaaaag ttaggagaat tgcgttgatg attacaatta gaaatttgag
    12421 caagacattc tcagggcaaa aagttttaga tagtttggct cttgatattg aaaaaggtca
    12481 agtgattgct ttagttggtg cttcaggagc agggaaatcg accttccttc gtagcctaaa
    12541 ctatttggaa aaaccagatt ccggctccat tagcattggt gactttacgg ttgattttga
    12601 gaccattacc acagagcaag tccttatttt acgtcggaaa cttgccatgg tttttcaaca
    12661 atttaatctt tttgagcgtc gtacagccct tgagaatgtc aaagaaggtc ttaaggtggt
    12721 caaaaaactg tctgaccaag aagcgactaa actggcgcaa gcagaattgg ctaaggttgg
    12781 tttagcagat cgaaaacatc attacccacg tcatttatca gggggacaaa aacaacgggt
    12841 agctttagca agagctctgg ctatgaaacc agatgtttta ttactagatg agccgacctc
    12901 ggctcttgac ccggaattag ttggcgaagt ggaaaagtct attactgatg ctgctaaatc
    12961 cggacagacc atggttttag tgagtcacga catgaatttt gtttaccaag tggcagatag
    13021 agtactcttt ttagatcaag ggaaaatctt ggaacaagga acacctgaag aagtctttag
    13081 acatcctcaa aaagaacgaa ccaaagaatt ttttgcaagc tattcaaaaa catacattta
    13141 atgttattct tttgttataa taaaaaggtc taggtattcc tgggcttttc ttgttatgtt
    13201 ttttagaaaa acttatggga aagaaataat gactaggtgg aagtatgtta gtagaaatct
    13261 ttagccttta tgtaaaaggt ctcattctct caagccttac tatgatttta atttgtttaa
    13321 tatggacttt atggcgagca aaaaccaaaa gagataaaac attgattgaa agacaagctt
    13381 ttctttatga gatgctaatg gttgctattt taaccatccc aattttatcg tttgctttta
    13441 tgagtatttt ggttgtcata aaatcctaat cattgcaaga agggattatc attgctaaaa
    13501 tattggcaag agtagattaa aggagaaaaa tcatgtacga tacattaatt attggttcag
    13561 gaccagcagg aatgactgcc gctttatacg ctgctagaag taatttatct gttgccatta
    13621 ttgaacaagg ggcacctggt ggtcagatga acaacacttt tgatatagag aattacccgg
    13681 gatatgatca tatatctgga ccagaattag ctatgaaaat gtacgaacct ttagagaagt
    13741 ttaacgttga aaacatttat ggtattgttc aaaaaataga aaattttggt gactataagt
    13801 gtgtgttgac tgaagatgct agttatgaag ctaaaacggt tattattgca acgggagcta
    13861 aataccgtgt actaggtgtt ccaggtgaag aatactatac tagtcgagga gtttcttatt
    13921 gtgcagtatg tgatggtgcg tttttccgtg accaggatct tttggtcgtt ggtggtggtg
    13981 actcagcagt tgaagaagct atttatctta ctcagtttgc taaaaaggta accgttgttc
    14041 atcgccgtga tcaactaaga gctcaaaaaa tactgcaaga tcgtgctttt gccaatgata
    14101 aggtcgactt tatctgggat tcagtcgtca aagaaattca aggcaatgat attaaggtct
    14161 ccaatgtcct aatcgaaaat gtgaaaacag gccaagtcac tgaccacgct tttggaggtg
    14221 tgtttatcta tgttggtatg aatcctgtga caggtatggt aaaggatcta gaaattaccg
    14281 attcagaagg ttggattatc acagatgatc atatgaggac aagtattcct ggtatttttg
    14341 cgattggaga tgttcgtcaa aaagaccttc gccaaatcac gacagctgtt ggagatggtg
    14401 caattgcagg gcaaggtgtt tatcactacc ttgaaagttt cccttcataa ggttaaaact
    14461 ccttcatttc tccttgtctt tcccttaaaa aagctaaatt gcataagacc tttaagaaat
    14521 ataaagaaca gttctcctta atatctttcc tagataaatg tt
//
LOCUS       DATURV010000041        13436 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DATURV010000041 DATURV010000000
VERSION     DATURV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13436
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.041"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..893
                     /locus_tag="VCF75_001184"
     CDS             <1..893
                     /locus_tag="VCF75_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP2546748.1"
                     /translation="KSEKVISTYHVTDDLKPFQVRDLVSGHLERIQEEVEKKYPNAGF
                     NSDKYNGLKESNSLLSDGFEVKSGNLIFDKKLTIPLTTLFDVINPDFLANSDRAAYDN
                     YRTYKEQHPKKLVALTFDDGPDPTTTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKR
                     VSDAGHEIANHTWDHPNLTNLSVSEIQHQVNMTNQAIEKACGKKPRYLRPPYGATNAT
                     VQQSSGLTQMLWTVDTRDWENHSTDGIMTNVKNQLQPGGVVLMHDIHQTTINALPTVM
                     EYLKAEGYECVTVSELYAHQ"
     gene            complement(1003..2088)
                     /locus_tag="VCF75_001185"
     CDS             complement(1003..2088)
                     /locus_tag="VCF75_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2546749.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2186..2812
                     /gene="udk"
                     /locus_tag="VCF75_001186"
     CDS             2186..2812
                     /gene="udk"
                     /locus_tag="VCF75_001186"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEP2546750.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            2892..3155
                     /locus_tag="VCF75_001187"
     CDS             2892..3155
                     /locus_tag="VCF75_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEP2546751.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERKLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3208..4017)
                     /locus_tag="VCF75_001188"
     CDS             complement(3208..4017)
                     /locus_tag="VCF75_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEP2546752.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     TEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4162..4659
                     /locus_tag="VCF75_001189"
     CDS             4162..4659
                     /locus_tag="VCF75_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEP2546753.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKESHLIGVLDLDSSLVADYDEVDQEYLEAFVDLLLEKTTFTFNM
                     FGVKN"
     gene            4659..6329
                     /gene="dnaX"
                     /locus_tag="VCF75_001190"
     CDS             4659..6329
                     /gene="dnaX"
                     /locus_tag="VCF75_001190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEP2546754.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISMLALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAQKEQILSQANLSGELISEIETLKNELAQLKQQLSQLQSRPD
                     SLARSDKTKPKTTSYRVDRVTILKIMEETVRNSQQSRQYLDALKNAWNEILDNISAQD
                     RALLMGSEPVLANSENAILAFEAAFNAEQVMSRNNLNDMFGNIMSKAAGFSPNILAVP
                     RTDFQHIRKEFAQQMKSQKDSVQEEQEVALDIPEGFDFLLDKINTIDD"
     gene            6424..6627
                     /locus_tag="VCF75_001191"
     CDS             6424..6627
                     /locus_tag="VCF75_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEP2546755.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKAANSPKKKD"
     gene            complement(6602..7543)
                     /locus_tag="VCF75_001192"
     CDS             complement(6602..7543)
                     /locus_tag="VCF75_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEP2546756.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEK
                     RAIAIDLTDQGHLIVQFDNGDLQTLRSGEISLSSWEN"
     gene            7747..10125
                     /locus_tag="VCF75_001193"
     CDS             7747..10125
                     /locus_tag="VCF75_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEP2546757.1"
                     /translation="MKTKKVIILVGLLLSSQLTLIACQSRGNGTYPIKTKQSRKGMMS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQQAASQGTSKVADSHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAHVADQYDLAKKAEKPAETHHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVEIQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNKM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKRLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLRQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPKLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            10661..11857
                     /locus_tag="VCF75_001194"
     CDS             10661..11857
                     /locus_tag="VCF75_001194"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEP2546758.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQICQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKIDALVE
                     AVKTVL"
     gene            12031..13290
                     /locus_tag="VCF75_001195"
     CDS             12031..13290
                     /locus_tag="VCF75_001195"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP2546759.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
BASE COUNT         4276 a         2316 c         2808 g         4036 t
ORIGIN      
        1 aaaaatcaga aaaagttata agtacatatc acgttacaga cgacttgaaa ccgtttcagg
       61 taagggatct agtatcagga catttagaaa gaatacaaga agaagttgaa aaaaaatatc
      121 caaatgctgg ttttaatagc gataagtaca atggcttaaa agaatctaat tctttattaa
      181 gcgatggctt tgaggtaaaa tcgggaaacc ttatttttga taaaaagcta acgatacctt
      241 tgacgacatt atttgatgtt attaatccag attttttagc aaatagcgat agagctgcgt
      301 atgataatta taggacctac aaagaacagc atcccaaaaa actagttgca ttaacgtttg
      361 atgatggtcc agatccgacg acgactcctc aagttttaga tattttggca aaataccagg
      421 ctaagggaac tttctttatg ataggttcaa aagttgtgaa taatgaaaac cttactaaac
      481 gtgttagcga cgctggccat gaaattgcta atcatacttg ggatcatccc aatctgacta
      541 atctttcagt cagcgagatt caacatcaag ttaatatgac gaaccaggct attgaaaaag
      601 cttgtggcaa gaaacctcgc tatttacgcc ctccatacgg agctacaaat gcaactgttc
      661 agcaatcctc aggattaaca caaatgcttt ggacggttga tacaagagat tgggaaaatc
      721 atagcactga tggcattatg actaatgtca aaaaccaatt gcaaccggga ggagttgttt
      781 taatgcatga tattcatcaa acaactatca atgctttgcc gacagttatg gaatatctaa
      841 aagcagaagg atacgaatgt gtgactgtat cagaactcta tgcgcatcag taagtaatat
      901 cgttatgttt ctttagaatt gatatactaa acaatccaat tactttgaag taattggatt
      961 gttgttttta gtagcccttg attaagtttt gttttttaac aactagttga tgaaatgaag
     1021 ttgttgattt ttgagggaga tttcagagac tttagcaaat tttttcaatt ttttgaggtc
     1081 ttcagggtgg ctaacaaagg taataacgat accagatttt cccatacggc cggttcgtcc
     1141 agctctgtga gtataatttt ccttatcacg agcaacatca aaatgaatga catagtcaag
     1201 attatcaata tcaatgcccc gagcaacaag atccgtagct aacaataagg agagttggtg
     1261 ggatttgaat ttttcgagaa tggttttacg gaatttgaca ttgatgtcac tcgctaagga
     1321 aaccgcagca gctccattat attggagacg ttcttccgta gcacccaaat cagataaact
     1381 gttgaaaaag actaaagctc taaaatgagg aatattagca aattttcgta ataaatcggt
     1441 acgttcgcgc ttgtcaacca tgaggtaaaa atgctgaata gcatcgttgg tttgctctga
     1501 aagatcgatg cagaatgtat ttggagctaa ggaagtttga tccaccttat tagtagcact
     1561 catgtatacc atttgatgat ctcgaggaac atagtggcta attttttgaa caaaatcata
     1621 ttgggaatca cctaatagtt cgtcatattc atctaaaaca atggtgttga cagacatcat
     1681 cttgattttt ttcaatttaa tcagttcaaa aatgcgtcca ggggtcccaa tcaggatttc
     1741 aggacctttt ttgaggcgtt cgatttgacg tttttgactg gttccagaaa taaagagttg
     1801 tgccgttaag cctagtggct gtgcccaatc ctttgtgact tcaaaaattt ggccagctaa
     1861 ttcggtatta ggggctaaaa tgagtagttg ttgggacttt ttaggcgtca gtgccaatag
     1921 actaggaaag acataagcta aggtcttgcc cgtcccagta gggctaatac caaggaaatt
     1981 tttaccatca acaataggtt gaaaggcctg ttcttggatt ggcgttaaat gggtaaatgc
     2041 aacttggtca agtttttctt gccattgagg gggaaattta gtgatcatgc gtcttccttt
     2101 tactattgtt tctatattca ttatagcatt atagtctcaa ttttggtaaa atagtgagaa
     2161 agacttttta gatttggagt ttaaaatgct taaaaaaccg attattattg gtgtaactgg
     2221 tggttcaggt ggcggtaaaa caagtgtgtc acgcgctatt ttggacagtt ttccaaatgc
     2281 tcgtattgcg atgattcaac acgattctta ctataaagat cagtctcaca tgagttttga
     2341 agaaagggtc aaaaccaatt atgaccatcc tctagctttt gatacagatt ttatgattca
     2401 gcaactaaaa gaattattag ctggtagacc tgtagatatt cctatctatg attacaaaaa
     2461 gcatacccgc agtaatacga cctttcgtca ggatcctcag gatgttatta ttgttgaagg
     2521 tattcttgtc ctagaagatg agcgtttgcg agatttgatg gatatcaagc tctttgtaga
     2581 cacagatgac gatattcgta ttattcgtcg tattaagcgt gacatgatgg aacgtggcag
     2641 aagcttagaa agtattattg atcagtacac aagcgtggta aagcctatgt atcatcagtt
     2701 tattgagcca agtaaacgct atgctgatat tgttattcca gagggagtta gtaatgtcgt
     2761 tgctattgat gttattaata gtaaaattgc gagtattctt ggagaagtat agtggaagtg
     2821 gctgagtaga ttatttaaat ctgctctttt attgttgtgc tcttcataga gaaagggatc
     2881 ataaaataga aatgttatta atttggcttg gattactatt tgtttggaat gaactcgtgt
     2941 ttgtcatata tgcctttgat aaaagaaaag ccataaaaaa gaaacggcgt attagtgaaa
     3001 gaaaattatt agtgataaca gttttgtttg gaggttttgg agcattactt gcagctaaaa
     3061 agtaccacca taaaaccaga aagtggtact ttgtcatcac ttggtatact agtattttgt
     3121 tgacattact agtaacttat atctttatga agtgagtcac ttaaaagacc cttaatcagt
     3181 attatttagt caaggctctt ttatagttta aaaaagcaca tcatcgtcaa gggtgttgat
     3241 tttttgaaaa agatcaccaa agattaaaag gattaatgtt cctagtaaaa ctgtcatgcc
     3301 aggaatgctt gttagaggag agttccagat gaaatgtaga accagaggac ttaataacca
     3361 gaaaatttgt tgctgttttg aaaaaaggtt agaacccttt aataataagt atgctccaat
     3421 ggtaaaaata gctgtatgta cccaatgtga agtcacagca gtgctaatac gttcaatagc
     3481 agtcggtacg agactatcta tagattttgc ttgaatcaaa taacggatat cttctgttag
     3541 ttgaaagccc atgccagaaa gtaaagcaac aactaatttt tctttaagag taagtctagg
     3601 aaataaagta attactaaaa tagccgaaga agccttaatg ggctcttcga caataggggc
     3661 agttaaagca tctccccaaa gctttaaagc gtctttagaa gggatgatgt atgaccaaaa
     3721 ttgtcctacg agatggttcc cacaagaagc taaaaaacct gaaatataaa gtccgctaag
     3781 acagctaaca ataataacgt tcaggttcat atgaaatttt ttacttaaat aaaaaatagc
     3841 tgctgcaaat ggaattaagt aaatgcctaa tagactaatg gttagtatta gagcaaaagc
     3901 ttctttaaag gaaaggttgt tagctgtcag tcctaacaat tcaagttcaa taccatttaa
     3961 aactaaagca ataaaaagac cgtatcgtaa ccatttctgt tgagtgattt gtttcacgaa
     4021 aacctcctaa tattaagatg atattaataa tattgaaaat tatatcataa acaacctcaa
     4081 aaaccatttt aaaaactgac tgaagcgaaa gatagtcgta aaaaatactc gaatagaccc
     4141 agatatgcta taataaaagg tatgaataag tctaaaaaaa ttgagcaata ccaactaatg
     4201 atagcgcagg caaaagagct gtttgcgaat gaatcaaatg ctttggctaa tttgtcaaat
     4261 gccagcgccc tgttaaacat gacactaccc aattctgttt ttgcgggatt ttaccttttt
     4321 gatgggcagg aattaattct tggccctttt caaggtaggg tttcttgcgt ccatatcaag
     4381 cttggtaaag gtgtttgtgg cgaatcagct cagtctcgtc gaaccattat tataaatgat
     4441 gtgaagcagc atgccaacta tatttcctgt gatgcggcag ccatgagtga aattgttgtg
     4501 cctatggtga aagaaagcca tttgattggg gtgctggact tggattctag ccttgtagct
     4561 gattatgatg aggttgatca agaatattta gaggcctttg tggacctact tcttgaaaag
     4621 accacgttta cctttaacat gtttggagtt aaaaactgat gtatcaagct ctttatcgga
     4681 aataccggag ccaaacgttt gacgaaatgg tgggacaatc ggttatttcc acaactttaa
     4741 agcaggcagt tgaatctggc aagattagcc atgcttattt attttcaggt cctagaggga
     4801 ctgggaaaac aagtgcggca aagatttttg caaaggccat gaattgtcct aaccaagtcg
     4861 atggtgaacc ctgtaatcaa tgcgatattt gccgagatat cacgaatgga agcttggaag
     4921 atgtgattga aattgatgct gcctcgaata atggtgttga tgaaattcgt gacattcgag
     4981 acaaatcaac ctatgcgcca agtcgtgcga cttacaaggt ttatattatt gatgaggttc
     5041 acatgttatc aacaggggct tttaatgcgc ttttgaaaac tttggaagaa ccgacagaaa
     5101 atgttgtttt tatcttggca acaacggaat tgcataaaat tccagccact attttatctc
     5161 gtgtgcaacg ctttgaattc aaagctatta agcaaaaagc tattcgagag catttagcct
     5221 gggttttgga caaagaaggt attgcctatg aggtggatgc tttaaatctc attgcaaggc
     5281 gagcagaagg aggcatgcgt gatgctttat ctattttaga tcaggctttg agcttgtcac
     5341 cagataatca ggtcaccatt gcaattgccg aagaaattac aggttctatt tccatgcttg
     5401 ctctgggtga ctatgttcga tatgtctccc aagaacaggc tacgcaagct ctggcagcct
     5461 tagagaccat ttatgatagt gggaagagca tgagccgctt tgcgacagat ttattgacct
     5521 atctgcgtga tttattggtg gttaaagctg gcggcgacaa tcaacgtcag tcagctgttt
     5581 ttgataccaa tttgtctctc tcgatagatc gtatattcca aatgataaca gttgttacta
     5641 gtcatctccc tgaaatcaaa aagggaaccc atcctcggat ttatgccgaa atgatgacta
     5701 tccaattagc tcagaaagag cagattttgt cccaagcaaa cttgtcagga gagttaatct
     5761 cagagattga aacgctcaaa aatgagttgg cacaacttaa acaacaattg tcgcagctcc
     5821 aatcgcgtcc tgattcactg gcaagatctg ataaaacgaa acctaaaacc acaagctaca
     5881 gggttgatcg ggttaccatt ttgaaaatca tggaagaaac ggttcgaaat agccaacaat
     5941 ctcgacaata tctagatgct ctaaaaaatg cttggaatga aattctagat aacatttctg
     6001 cccaagacag agccttattg atgggctctg agcctgtctt agcaaatagt gagaatgcga
     6061 ttttggcttt cgaggctgcc tttaatgcag aacaagtcat gagccgaaat aatcttaatg
     6121 atatgtttgg taacattatg agtaaagctg ctggtttttc tcccaatatt ctggcagtac
     6181 caaggacaga ttttcagcat attcgtaagg aatttgctca gcaaatgaaa tcgcaaaaag
     6241 acagtgttca agaagaacaa gaagtagcgc ttgatattcc agaagggttt gattttttgc
     6301 tcgataaaat aaatactatt gacgactaaa actgaaatac ataagggtaa ggttttgccc
     6361 ttacccacgt tttatctcgt tacttttttt catagtacag tatttttttg ttataataaa
     6421 gctatgacta ttcgacaatt tctttttatg gcctttgtat gtgcctttga aacctatttt
     6481 tttaatgacc tgttgctctc tggaaattat ctcttcgcct tattttgggg cttgttgctc
     6541 tttagggatt taagaagagt gcataccatt aatcaattga ctaaaaccat tttaaaagcg
     6601 gctaattctc ccaagaagaa agactgattt caccgcttcg caaggtttga aggtcaccat
     6661 tgtcaaattg gacaataagg tgtccttgat ctgtgagatc aatcgcaata gcacgttttt
     6721 cttgactatt ttctataaag gtcacttgtt tattaagaac aagtgatttt tctttgtaaa
     6781 ctttgacatg gtccttgaca ggaatagata agaataattt ccagatgtca atgataaggt
     6841 cattgcgcgt gatagtcggt ttttcggtaa agagagaacc tgccttttga gcaatcgctt
     6901 ctgggaagtc ggtgacaaaa aagttgagac caaccccaat aatgacatcg gtgattaaac
     6961 cagtttcaac agaagtaatg gcttctgtca gaatccctgc aactttatgg ttacctagat
     7021 agatgtcgtt tacccatttg atttctgtat caatgcctgt taatcgcgag atagctttga
     7081 caatactgga agcaaccatc atcgtataag gaggcatatc agcataaggg acattgggtt
     7141 ttaggtacat ggacatgtaa ataccaccgg tgctagctga aaaaaattgc cgatcgaggc
     7201 gtcctttggc agcttcttgg ctaggagcta ggtaaagcct tggagcgctg ttatgagctt
     7261 cgattccttg tttggcatct aattgggtag attggctgtg aggggtatag gtgactggca
     7321 tcccaagccc ttggctaatg gttttgggta ataaaatatc accttgtacc atgcggtagc
     7381 ccttgtgttt taaagaatca atttgaatcc cctgattttc gagacttttg atggatttcc
     7441 aaacggatgt acgagagata ctgagttgat ctgctagata ttccccactg acgaagtcat
     7501 cggtttgtga cagtaattga taaatttttt ctgatgtttt catatgtata gtatattata
     7561 aaagatctta ttttcaaagg aaaagaaaag ttagtttttt tgtgtaatgc tactaacaac
     7621 agaaactatt gtttgttgaa gcttcagagc tccaactcgt caacaactaa tagttatcct
     7681 aaaaagagct tgaggaaaaa caagtgatgt gatatactta accagttaaa taaaaaggag
     7741 ggttgaatga aaacgaaaaa agttattatt ttagttggtc tattgttatc atctcagttg
     7801 actttgatag cttgtcaatc acgaggtaat ggtacatatc ccattaaaac gaaacaatca
     7861 cgtaagggaa tgatgtcaaa caaaattaaa ccgattaaaa aaagcaaaaa gacaaacaag
     7921 actcacaaag gtgtggcggg tgtcgatttt cctacagatg atgggtttat tttaaccaaa
     7981 gactcaaaaa tcttatcaaa aacagatcag ggaatcgttg ttgaccatga tggtcattcg
     8041 cattttattt tttatgccga tttaaaggga agtccatttg aataccttat tccaaaagga
     8101 gcaagtttag ctaagccagc tgttgctcag caagcagcta gtcaagggac ttctaaagta
     8161 gcagattctc atcaccatta tgaatttaac ccagcggata ttgtggctga agatgcttta
     8221 ggctacacgg ttcgccacga tgatcacttc cattatattt tgaagtcaag cttatcaggt
     8281 cagacacagg cacaagctaa acaggttgct actcgcttgc cacaaaccag tagccctgtt
     8341 tcaacagcta cagctaatgg tattccaggc ttgcatttcc caacctcaga tggttttcaa
     8401 tttaacggtc aaggtattgt tggggtaaca aaagacagta ttttagtgga ccacgatggt
     8461 cacttacatc ctatttcttt tgcggacctt cgtcagggtg gctgggcaca tgtggcagat
     8521 caatacgatc tcgctaaaaa agcagaaaag ccagcagaaa cccatcacac accagagcta
     8581 tctgaacgtg aaaaggaata ccaagaaaaa ttagcttatt tggcagaaaa attggggatt
     8641 gatccatcaa ctattaaacg tgtggaaata caagacggta aacttggttt ggaataccct
     8701 caccatgacc acgcacacgt attgatgtta tctgatattg aaatcggaaa agacattcca
     8761 gatccacatg ctattgagca tgcccgtgaa ttggaaaaac ataaggttgg aatggatacc
     8821 ttgcgtgcct tagggtttga tgaagaagtg attttggata tcgttcgcac tcacgatgct
     8881 ccaaccccat tcccatcaaa tgaaaaagat ccgaataaga tgaaagaatg gttagcaacg
     8941 gttattaaac ttgacttggg cagccgtaaa gatcctttgc aacgtaaagg actttcactg
     9001 ttacccaact tagaaacttt aggaattggc tttacaccaa tcaaagatat ctcacctgtt
     9061 ttgcaattta aaaaattgaa acggttacta atgacaaaaa caggggtgac tgattataga
     9121 tttttggata atatgccaca gttagaaggc attgatattt cacaaaacaa tctcaaagat
     9181 attagtttct tgagcaaata taaaaactta actctagtag cggctgctga taatggtatt
     9241 gaagatatta ggccgcttgg tcaattacca aatctcaaat tcctcgtatt gagtaataat
     9301 aagatttctg atttaagccc actggcatcg ttacgtcaat tgcaagaatt gcacattgat
     9361 aataatcaga ttacagattt aagccctgtt tctcataaag aatcattgac ggttgttgat
     9421 ttatcaagaa atgctgatgt tgacttagca acacttcaag cccccaaatt agaaacgtta
     9481 atggtcaatg ataccaaggt ttctcatttg gatttcttga aaaataatcc taatctatct
     9541 agcctatcta ttaaccgtgc gcaattgcaa tctcttgagg gtattgaagc aagtagcgtc
     9601 attgtcagag tagaagcaga aggtaaccaa attaaatcgc ttgtgcttaa agacaagcaa
     9661 gggtcactta ctttcttgga tgtgacaggc aaccagttga cttctctaga aggtgttaat
     9721 aattttacag cacttgacat tttaagcgtg tctaaaaacc aattaacaaa tgtcaaccta
     9781 tctaaaccca ataagacagt tactaacatt gatattagtc ataacaatat cccattggca
     9841 gaccttaaat tgaacgagca acatattcca gaagccattg cgaaaaactt cccagcggtt
     9901 tacgaaggtt ctatggtagg taatggaaca gctgaagaaa aagcagctat ggctactaag
     9961 gcgaaagaaa gtgctcaaga agcatcggaa tcacatgact acaaccataa tcatgcctat
    10021 gaagatgaag aaggtcatgc tcacgagcac agagacaaag atgatcacga ccatgaacat
    10081 gaggatgaaa atgaagctaa agatgagcaa aaccatgctg actaaactaa tctaagaata
    10141 acccgatcca actgaatcgt cttaatgaca ccattgttga ctatcaagaa aacctcaaga
    10201 ataaccttga ggtttttttg aaataaatag gataatgagt gaaaagaatg gcacctaaat
    10261 gactaaaaca tgttataata gattgttatt gagtcccgat aagacggtct gttgaacgaa
    10321 atgttttgag acgttgttga cttcatttgg ggaacagtcg ttctctcttt agctaaatta
    10381 cttcgtctaa ggcttatttt gtggtgacat cctctcaaca aggagaccgg attctttgta
    10441 actcaacgct attgtcagac attctttgtc tggttaatgg acgctagcca ctaggtggac
    10501 tagctagttg ccttactagt aaaaccgtaa ctgattagct aagttggttt tggccacttg
    10561 acttgctcaa tcagctagga agaaaggggt aggcaacttc ttgctttgtg gtcaatgtga
    10621 ctgaaaaagg aagaatcaac atattagggg ggacattttt atgtcagaac gtaaactttt
    10681 cacgtctgag tccgtatctg aagggcatcc agataagatt gcagaccaaa tttcagatgc
    10741 tattttagat gccattttag cagaagatcc agaagcccat gtggctgcag aaacttgtgt
    10801 ttacacaggt tctgttcatg tttttggaga aatctcaaca acagcataca ttgatattaa
    10861 ccgtgtggtc cgcgacacca ttgcagaaat tggttataca gaagcagaat atggcttttc
    10921 agcagaatca gtgggagtac atccatcact tgtagaacaa tctggagata tcgctcaggg
    10981 ggttaatgaa gctctcgaaa gcagagaagg tgatactgat gaccttagcc atattggagc
    11041 gggtgaccaa gggctcatgt ttggttttgc catcaatgaa acaccagaat tgatgccttt
    11101 accaatttcc ttatctcacc aattagtgcg tcgcttggcg gaattacgta agtcaggtga
    11161 gattagttat ctacgtccag atgctaaatc tcaagtgaca gttgagtacg atgaacacga
    11221 taaacctgtt cgtgtggata cagtcgttat ttctactcag catgatccag aagctactaa
    11281 tgaccaaatt tgtcaggatg tgattgaaaa agtgatcaag gctgtaattc cagcagatta
    11341 tttagatgat gacacaaagt tcttcattaa tccaacagga cgttttgtta ttggaggtcc
    11401 gcaaggagac tctggattga ccggacgtaa gattattgtg gatacctatg gaggctattc
    11461 acgtcatggt ggcggtgctt tttcaggaaa agatgcgaca aaagtggacc gttcggcttc
    11521 ttacgctgct cgttatattg ctaaaaacct tgtggcagca ggccttgcga cgaaagctga
    11581 agtccaacta gcttatgcga ttggagttgc tcagcctgtg tctgttcgcg tggatacatt
    11641 tggaacttca acggttcctg aagctgtttt agaagctgct gttcgtcaag tctttgattt
    11701 acggccagca ggcattattc aaatgcttga tttaaaacgc ccgatttaca aacaaacagc
    11761 tgcctatggt cacatgggga gaacagatat tgatttgcca tgggagcgtt taaataagat
    11821 tgatgcttta gtagaagctg taaaaacagt attataaacg acaactttat taaaaaagta
    11881 gagtgagtga aaaaatctta ctctgctttt ttgttattaa ataagccttt aaaagggatg
    11941 attaaaaatt aagacgtcaa taattgtcag gcttcatgat ttatgataaa atgaaaaggt
    12001 tgaattctag tataagaaag ttttgaatgt atgcgtaaaa ttattattaa tggcggaaaa
    12061 gccttatctg gagaagtggc agtttcaggg gcaaaaaaca gtgttgttgc gcttattcca
    12121 gcaatcatct tagccgatga tattgtgatt cttgatggtg tacctgctat tagtgatgta
    12181 gatagtctga ttgaaatcat ggagcttatg ggagcaacag taaactatca tggtgatact
    12241 ttagaaattg accctagagg ggtgcaagac atcccgatgc cttacggaaa gattaatagt
    12301 cttcgagctt cctattattt ctacggtagt cttttaggcc gttttggaca agcagttgtt
    12361 ggcttacctg gtggttgcga tttaggacca agaccaattg acttgcattt gaaggccttc
    12421 gaggctatgg gagttgaagt gtcttatgag ggtgaaaata tgaatttatc gacaaatggt
    12481 caaaaaattc atggcgctca tatctatatg gacaccgtta gtgtcggtgc tacaattaat
    12541 actatggttg cggcaacaaa agctcaaggt aaaacggtta ttgaaaacgc tgcacgtgag
    12601 ccagagatta ttgatgtggc tactcttttg aataacatgg gtgcgcatat tcgtggagca
    12661 ggtacagata ttattacgat acaaggtgtt cagaaattgc atggtactag acaccaagtt
    12721 attcctgatc gtattgaagc agggacttat atcgctttgg cggcagctat tggaaaaggt
    12781 gttaagatca ctaatgttct ttatgagcat ttagagagtt tcattgctaa gttagaagaa
    12841 atgggtgtcc gtatgacggt agaagaggat gctatctttg ttgaaaagca agaatccctc
    12901 aaggctatta caattaaaac ctctccttac cctggttttg ccactgattt gcaacagcca
    12961 ttaacgccct tgttgttaaa agcagatggt cgtggaacga ttattgacac tatatatgaa
    13021 aagcgcatta accatgtgcc agagttgatg cgcatgggag cagatatctc agttattggt
    13081 ggtcagattg tttatcaagg gccgtctcgc ctgacaggag ctcaggtcaa agccactgat
    13141 ttacgtgccg gagcggctct agtgactgct ggtctaatag ctgaaggcaa gacagaaatt
    13201 actaatattg aatttatctt acgtggctat gctagtatca ttgcaaaatt aacagcacta
    13261 ggggcagata ttcaattaat cgaagactaa agagaagcta acgcttctct tttttatatt
    13321 acatgtgctt aggctaataa tacttaggtg catactttgt tgcattttat ggctttttaa
    13381 ctgtcataaa aaatataaaa atgatatcac taactaatat tattaatttt aatgct
//
LOCUS       DATURV010000042        13296 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DATURV010000042 DATURV010000000
VERSION     DATURV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.042"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..845)
                     /locus_tag="VCF75_001196"
     CDS             complement(<1..845)
                     /locus_tag="VCF75_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546760.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAETSTLTP
                     QGRQEKIKKIKDHITQYPKINEDRFWDRNWLRNSFKDEDYLKAVQNFTEQALQEILDL
                     IEKGATKGDKGDAGPRGERGPQGPEGKQGKAGERGAQGPKGDRGEQGIQGKPGEKGDK
                     GETGERGEKGETGATGAQGPQGEAGKDGAPGEKGEKGDRGETGAQGPVGPQGEKGETG
                     EQGPAGPQGEAGKPGEQGPAGPQGEAGKPGEKAPEKSPEVTPTPEMPEQPGEKAPEKS
                     PEVTPT"
     gene            1227..1709
                     /gene="nrdI"
                     /locus_tag="VCF75_001197"
     CDS             1227..1709
                     /gene="nrdI"
                     /locus_tag="VCF75_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP2546761.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(2103..2921)
                     /locus_tag="VCF75_001198"
     CDS             complement(2103..2921)
                     /locus_tag="VCF75_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEP2546762.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPNIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETDYRTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLDWR"
     gene            complement(3004..5190)
                     /locus_tag="VCF75_001199"
     CDS             complement(3004..5190)
                     /locus_tag="VCF75_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP2546763.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDHNTQATPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKVGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGALIPEVNMSQLT
                     SKPTPAKDFKHVTEDVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTTEIQDVTLTSLGNQPAKTKVATVEL"
     gene            complement(5547..6296)
                     /locus_tag="VCF75_001200"
     CDS             complement(5547..6296)
                     /locus_tag="VCF75_001200"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEP2546764.1"
                     /translation="MQQYFIKGKAEKEVTITDKDTIKHMFQVMRLADEAEVILVFDDG
                     VKYLAKVTNSMAHELEIIETLPDQVELPVEVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEQKAEFLKSLSS
                     FDHIFIAYEETAKAGQLATLAREVKEVKSGVKLLFIFGPEGGISPAEITQFEAAGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(6296..7249)
                     /locus_tag="VCF75_001201"
     CDS             complement(6296..7249)
                     /locus_tag="VCF75_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEP2546765.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIEQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQILRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMIQGEWNALVFKKTDDISGVIGG"
     gene            complement(7320..7790)
                     /locus_tag="VCF75_001202"
     CDS             complement(7320..7790)
                     /locus_tag="VCF75_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEP2546766.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKADFFLEWNAQAFEEGQQGLEEAASYPYPRY"
     gene            7991..9748
                     /gene="pabB"
                     /locus_tag="VCF75_001203"
     CDS             7991..9748
                     /gene="pabB"
                     /locus_tag="VCF75_001203"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEP2546767.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKSLDQVGPVIENVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDEFAVISASPELFFKQEGNQLTTRPMKGTTKRGVNTWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQIGSVCVDRLCEVERYSTVWQMTSTIVGDLKADCDL
                     IDILKALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVQQKTVFLYRHKQIFDLKTTAKVEHQKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKDNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQVFYNQAGQLLETSIGN
                     LFIQLGQTLYTPPVAVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            9781..10347
                     /locus_tag="VCF75_001204"
     CDS             9781..10347
                     /locus_tag="VCF75_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEP2546768.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVFSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            10380..11648
                     /locus_tag="VCF75_001205"
     CDS             10380..11648
                     /locus_tag="VCF75_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEP2546769.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLKTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            11737..11933
                     /gene="ssrS"
                     /locus_tag="VCF75_001206"
     ncRNA           11737..11933
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VCF75_001206"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11934..12006
                     /locus_tag="VCF75_001207"
     tRNA            11934..12006
                     /locus_tag="VCF75_001207"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11967..11969,aa:Lys,seq:ctt)
     gene            12147..12587
                     /locus_tag="VCF75_001208"
     CDS             12147..12587
                     /locus_tag="VCF75_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2546770.1"
                     /translation="MKIIEYKGNYRDNLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            12642..13148
                     /locus_tag="VCF75_001209"
     CDS             12642..13148
                     /locus_tag="VCF75_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEP2546771.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVVHLDQLHFSSNWQERS
                     DHDMIADLSTCLLNQDWIIEGNYANCLYEERMSEANHIIYVNFSRFHCLYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVIQKYPHKTIVLTNQKQL
                     NHYMNTIN"
BASE COUNT         3698 a         3108 c         2632 g         3858 t
ORIGIN      
        1 gttggtgtca cttctgggct cttttctgga gctttttcac ctggttgctc tggcatttct
       61 ggcgttggtg tcacttctgg gctcttttct ggagcttttt cgcctggttt gcctgcttca
      121 ccttgtgggc ctgctgggcc ttgctcacct ggtttgcctg cctcaccttg tgggcctgct
      181 gggccttgtt cgcccgtttc acctttttca ccttgtgggc ctactggacc ttgagctccg
      241 gtttcaccgc ggtcaccttt ttcacccttt tcgcctggag cgccatcttt accagcttca
      301 ccctgtgggc cttgggcgcc agttgctccg gtttcacctt tttcaccgcg ctcaccggtt
      361 tcacctttgt cgcctttttc accaggttta ccttggattc cttgctcacc gcggtcacct
      421 ttaggacctt gggcgcccct ctcaccagct ttaccttgct tgccttctgg accttgtggg
      481 ccgcgctcgc ctctcggacc agcatcacct ttatcgccct ttgttgcacc tttttcaatc
      541 agatctagaa tttcttggag tgcttgttca gtaaaatttt gtactgcttt taaataatct
      601 tcatccttaa aagagttacg gagccagttc cgatcccaaa atctgtcctc atttattttt
      661 ggatattgcg taatatggtc tttaattttt ttaatttttt cttgtctgcc ttgtggcgtc
      721 agagtagaag tctcagcctt aacgctgctt gccccgcctc caaaggctag aaggacggca
      781 gcggctgagg ttagcccgta gcgccagacc agtttgttga gattatggtg ttttgatgtc
      841 aacatatgtt gttctctctt tctctattag atgatgtgtt ttagataaaa ataacatggt
      901 agctagacct gattatttat aacagtatta taatagagtg tgaaaacgat tgtaaagttc
      961 tcttttgttg ttcatctttt agattttgac tgattgctct tttgtggaga ttagagggcc
     1021 actttggcac ttgcctgcct cgtttttaag tcgagggcaa gcggatcctt tgtttaaggt
     1081 cactcttttt taaaatgtta ctttttttga ctgcttgtac cttcttgtct ggttggatac
     1141 cgctaggttt cttctttact tgtttgttgg tcttgattgt ggtaagctta tgaagaaacc
     1201 ttgtaggata aaaaaaggag acacttatgc ctcagattac ccttgttttt attagcctca
     1261 gtggcaacac ccttagcttt gtcaagcggc tatcccttta tctagcagac aattacgatt
     1321 accatgtcaa acagattaac attaaagatt tgaaacacga gacttttccc gtcaaggaag
     1381 agtttgtggc gattctaccc acttatctag aaggcggaaa tggcgttgac tctggtgagg
     1441 tggagatcct caccactcct ttgggagagt ttattgctgc ccatggcaac gcccagcgtt
     1501 gtcttgggat tattggctca ggcaacaaaa actttaacca tcagtattgc ctgacagcca
     1561 aacagtatgc caaacgcttt ggctttccac tgctaggtga ttttgagctg cgagggactc
     1621 ctgatgacat ttcgcggtta gcccaggtca tcatggaggc cagctccaga cactcgtcta
     1681 acgacacaca gacactgcct aacagctaga cagtctatag atgatcatag ctgttttaaa
     1741 aaagagcact tttaggacac caactaggga aggcccctac cccacgcctt gtgatttctc
     1801 agccttttaa aagagcgatc acagtgcttc ttggggcttg ggatcacacc aagcactcct
     1861 agaaatatcc ccatgcagac tttagcaagg caactgtacg cttggtggta ctttctgtga
     1921 agctcagatg gttgggggca aaaaagccct ggttttttag ggttactgac atgactaaag
     1981 aggtctaacc agctcaaggt agagctggtt agacctcttt gtgttctcct aatcccccta
     2041 gacgatcgca gtttcttatg tgtcttggct tctcctatgt ggtgtgcctg ttagcacatg
     2101 ctctaacgcc agtctaatgt cacttctaaa ccatagtggt cactgacgac tggcgcatcc
     2161 ccgccttcaa aggtaatctt agaagatcgg atgatgaagt ccttactggt aaagacatga
     2221 tccaccttga gggcttcttt gttaccttgc cagccatcaa tatcagccac gatgctatga
     2281 tcaccaaaca cgtggtcagc aaccttatgg ctatcttgta ggtctagggg gctacccatc
     2341 atcacttgat aaccgtcttg atccgttgga ttgttaaaat ctcccataag tagcaaggga
     2401 cagtttagcg tgagcagctc tttttctaat ttctcccact cccctaaaaa ccccttgtca
     2461 aaccatgaca agtgcacatt gacaacagcg acttctttgc catctaacgt ggttttggct
     2521 atgagtgagc gtctggtacg gtagtctgtt tcatcagcca tcgctgacac gagaatatct
     2581 gaaacatgaa taggttgttt tgataagatt gccacgcctt cctggtaaat gtcatagcca
     2641 atatggttat aagcccaaga ccaataatag tgctgccctc gtttttgtaa ataatggatc
     2701 aagagaagag caaaatggtc tttatgaatg tttggtgtcc ctgaaagagc ttgatatctt
     2761 ggtaaatctg tggccagctc gctctcgatt aactgattga tttcttgcaa gcaaatgata
     2821 tcgtattttt cagctaaaat atgctcagcc aaagcaacta attttttaag agtgttagct
     2881 tgcatccacg aatgggtatt cagtgttaat accttggtca ttaatcctcc tttataagac
     2941 aagaagagcc atagggccta aaccagctat ggctattacc tatcactatc tcgtgatgtt
     3001 ttgttaaagc tctacggtgg ctactttagt cttagcaggt tgattgccta aagaagtcaa
     3061 ggtcacatct tggatctctg ttgtgttggt aaaggctacc acgatagtgg tctcacgctc
     3121 tgctgattta atagcagcaa gatctgctac gactaatagg tcacctgcat gaacccgttg
     3181 cccttctgag actttgactg aaaaaggcac accatttaag gcgacggtat caagtcccac
     3241 gtgcaccaac acttcaagac cattatctgt cagtaaacca aaggcatgct tggtcggaaa
     3301 gacactggtc acaaggccag ctacgggtgc atagatatta ccatgggttg gttcaaccgc
     3361 aaacccatct cccatcatct tagccgcaaa cacctggtct ttcacacctg tgattgggag
     3421 aaccatcccg tctgccactg atagcacatc ttctgtcacg tgtttaaaat cttttgcggg
     3481 agtcggtttg ctagtgagct gtgacatgtt gacttctgga ataagagccc ctgagtccag
     3541 caaatcttga atatctgatt tcaaaatatc tgctttaggg ccatagaccg cttgaacgcc
     3601 gttgcctttt tggatcaagc ccatagcgcc agcttttttc caatcgtcct cagcgccaac
     3661 cttagcgggg tctttgaccg tcactcgtaa gcgcgtcata caagcatcaa catcatcaat
     3721 attgcctgcc ccaccaagga gattgatgat ttgtacgact tgagagttgc tatctgctac
     3781 ttgggtcggt gttgcttgag tgttatggtc acccagtata tcggcatcgt agttgcctaa
     3841 acgaccagct gttgcgagat gcattttctt gatcatcata tctgcaataa agtacatgat
     3901 aactgcaaag aggatagaaa cccaaacaaa gttaatcaca tccatgccta ggcctgcttt
     3961 aagggccata ggggtacggg tcaataattc aatgttccca aatgagtgaa cacggagatt
     4021 cacaaggtca gccatggcaa atgaagctcc ttgaacaagg gcatagacga tgtaaagtgg
     4081 cattgctgca aacataaata ggtactcaag tggctcagtt acccctgtca aaaagaccgc
     4141 tgccgctgct gagatgaaca tcattttgta tgtgtgtttt ttatcggcat ccacattacg
     4201 gtacatggct aaggcaaccc ccatcaatgt tccggtagct ccaatcattt gtcctacctt
     4261 aaagcgagct ggagtcacac tatccattaa gtgactatag gctgaagcgt ctgatccttt
     4321 aaggtgaaca aggtcagtta cccaagcaag ccacaaaggg tcttgaccaa atacttttgt
     4381 ccccgctgct gcacctgtca tgacctcata agttccacca agagctgtat agttcattgg
     4441 gatcgtcaac atgtggtgaa gaccaaatgg caacaagaga cgttccaagg taccatatag
     4501 gaatggggct aggattggag ctgaatcttg tgaagaggca atccacatcc caaaactgtt
     4561 aatcccagac tggatcaccg gccaaacaac aaccaaaata agggctacaa aaatagaacg
     4621 taaaataacg acaaacggta caaaacgctt cccattaaag aaggtcaaaa cttcaggaag
     4681 tttacggtaa ttatagtatt tgttataggc cgttgctcct acgaaccctg caataatccc
     4741 aacaaaaacc cctgtgttta aagctggaga ctccaagaca ctggtgaaat aatctttaac
     4801 gatcatttga gtgccaagga ggcttgtgat ttttgcttct ggatcagcta gcatagcact
     4861 agacacgcca taaaaagcgc ctgttatccg attgatcaag acaaaagcaa gccctgaggc
     4921 aaaggcccct ccagctcttt ctttagccca actaccacca atggccaagg caaatagcaa
     4981 atgaaggtta acaataacag cccagccaat ctgagcgata atgttgccta gagatgccaa
     5041 aaatgctgag tcatggttaa tcattggaat agagtttccg atactgatca ttaaaccagc
     5101 cgctggcatg acagcaataa cgaccataag acatttccca aatttttgcc aaaattcgaa
     5161 acgaaacagt tgcttgaatg atgttttcat tctatctctc ctttattatc cccttttagc
     5221 catagctgcc cttaaggcgc ttttgtgaca caatgatata gactacaact cagtgatgca
     5281 atcgtttgca ttggtctgat acctttatta tactagcctc gcaacaattt gtaaagcgtt
     5341 ttcttagaca gatgttagtc gtttttcttc attaaatggt agtgtaactt atcaaaaaga
     5401 tgacaagagc ccatttcctt ttttggtgat gagcccatca acgctcttca aaaaaacaag
     5461 aagcaataaa aaaaggccgt tgacacaatc gtgaactgcc tgttcgtttc tttagcctct
     5521 tctttctaaa gtaagcaaca gcttacttat tttgatgcct gcaattccaa agcataacta
     5581 agagcactta aggcatataa cggcgctgtc tctgctcgca taatgcgagg gcctaagccc
     5641 accttgatgg cacctgctgc ctcaaactga gtgatttcag ctggggaaat tcccccttct
     5701 ggtccaaaga taaagaggag ttttacccct gatttaactt cctttacttc acgcgccaag
     5761 gtcgctagct gacctgcctt agctgtttcc tcataagcaa tgaagatatg atcaaagcta
     5821 gaaaggcttt ttagaaactc tgctttctgt tcaaaaaggt gaacttcagg caccctattt
     5881 cgtttgctct gctcagccgc tcctaataca atcttagcca atttgtcttc ttttttagct
     5941 aattttttgc catcccattt gaccactgac cagtctgctg ggtagcccca tagcgcactg
     6001 gctcccaatt cagtcacttt ctgggcaatc gtgtctaact tatctccttt aggaaatcca
     6061 gaagcaatgg tcacctcaac aggcaattcc acctgatctg gcaaggtctc tataatctct
     6121 aactcatgag ccatactatt ggtcaccttg gctaaatact tcaccccatc atcaaagacc
     6181 aagataactt cagcctcatc agccaagcgc atcacttgaa acatgtgctt gatggtatct
     6241 ttatcggtaa ttgttacctc tttttctgct ttgcctttaa taaaatactg ttgcattagc
     6301 caccgatcac tcctgagata tcatctgttt ttttaaagac aagggcgttc cactcgcctt
     6361 gaatcatgtg tgtctcaagg aagaatcctg cgctaaaggc agcttccaga accatatcca
     6421 atttctcaga aatgatgcct gataggataa ggtaaccttc ctttttgacc aaacggtaag
     6481 catcatcagt caataagact aagatatctg ccaaaatatt agccacaatc acatccgctt
     6541 cttggctaac cccttttaaa aggtcgccag cagccacatg aatgttgtct gttccttgat
     6601 tcaaatcaat gttttcttga gccactcgaa cagccacgtc atctaagtca taagcgtaga
     6661 tggttttagc acctagtaag gaactggcaa tcgagaggac acctgatccg gtccctacat
     6721 cgataactgt ctcaccacca cgaaggattt gttccaaggc aaaaaggctc attttagttg
     6781 tgggatgtgt ccctgtccca aatgccattc caggatctaa tttaatcact ttttccccag
     6841 cagaagcgtc ataatccgtc caactaggga caatggttaa atcatgcgtg atgcgagctg
     6901 gttcatagta ttttttccaa ttgtctgccc agtcttcctc agctaattct tgactgtcca
     6961 cagtaacctg accaacctgt aacccaaaac tagctaactc cgctaactgt tcattaatgg
     7021 tcgcaataac atccgccaaa ttagtgctac ttgggtaata ggctgtgata gctatcatat
     7081 cagactgctc cacatctggg tataactccc caaagcggtc cttttgctca atatagtctg
     7141 cgctatctgc gatagcaacc ccttggctgc ctgtctcaat caacacatgt gaaacagcct
     7201 cttgcgcatc acgatgcaca tgaaccgtca cttcttgcca cgtttccata agtatgtcct
     7261 ttctattttg accttttggt tattgtccta atagtgttag caccatctcg tgtgtcacct
     7321 tagtaccttg ggtaagggta acttgctgct tcttctagtc cttgttgacc ttcttcaaaa
     7381 gcttgcgcgt tccactccaa gaagaaatca gccttgctgt catctgctaa gaaatccatc
     7441 aaatcgctgt gcccttcaat agcaacatcg ttgagaaatt gcgcaaagta agctaagaat
     7501 tcacgagaaa agcccttttt ggcgtcaaaa ggaatagtaa ccaaataatc tgctgcatca
     7561 aattgagact tggcagggtt gtaaaagagc acatagtcct caaagacaat atcatcttgg
     7621 gtcacttcgc cactatcatc aatggttttt atggcctctt tattttgagc ttctaaaaca
     7681 aaagtcactt ccaccgcgtg atttttcttg tcccaatcca tagcatagtc atactgaaaa
     7741 tgcttatcca tttcttcttc taatactgat aaaaagccgt attttgccat ctttttttat
     7801 acctaatctt tctaactgtt gctgtataga gctttgtaag acctcattag tttacttact
     7861 ttgacatgct gagcgtactg ttataacttt tattaactga attataacat atttgactaa
     7921 tattgaccat aagagaaggt cttaatggca ggatcacctc tagcaaccgc ctttatgata
     7981 taatttgggt atgcatagaa aaacgattat tgacttcaaa gagctcgggc aacgctatct
     8041 ttttgatgag ccattagtag aattggtagc caagtcctta gatcaggtcg gtccagtcat
     8101 tgaaaacgta caacactacc aacagctggg ctattacgtg gtgggctatc tcagttatga
     8161 agcagctgct ttttttgaca atgccctaca aacccataat gacagactag gcaacgagta
     8221 ccttgcctat tttacagtgc acaaaacctg tcaaaaaaaa gacctacctc ttgattatga
     8281 cagcattact atccctaacc aatgggtcag cgcaactcaa aaagaagcct atcaaaaagc
     8341 cattgaaacc attcaccgtg agatgcaaca gggcaatacg tatcaggtta actacaccct
     8401 tcaattaact caggaactca atgcagctga cagcttagcc atttacaata aactcgtcgt
     8461 tgaacaggct gctggctaca atgcctacat cgcccatgat gagtttgccg ttatttcagc
     8521 tagtcctgaa ttgttcttta aacaggaagg gaatcaactc accaccagac ctatgaaagg
     8581 caccaccaaa cgaggggtta acacctggct ggatcaacaa gaacatgact ggctccaagc
     8641 tgacgggaaa aatcgctctg aaaacatgat gattgtggat ttgctccgca atgacatggg
     8701 gaaaatttgc caaataggca gtgtttgtgt tgataggctc tgtgaggtcg agcgatactc
     8761 taccgtctgg caaatgacct caaccattgt tggtgacctc aaagcggact gtgacctgat
     8821 agacattctc aaagctctct ttccttgtgg ctctatcaca ggggctccta aagtctccac
     8881 catggcaatt atcacatctc tggagccaaa accaaggggg atttattgtg gaagtattgg
     8941 catttgccta cctgacggtc gccgtttctt caatgttccc attagaacca tccagctcag
     9001 tcataatcaa gctacttatg gtgtcggagg cggtatcact tggcaaagca agtgggaaga
     9061 cgaatacgaa gaagtccagc aaaaaacagt ttttctctat cggcacaaac aaatctttga
     9121 cctcaaaaca accgccaagg tggaacacca aaaaatagcc ttccttgagc aacacctcaa
     9181 tcgccttaaa gaagcagcca cttattttgc ttacccttac gatgagaaag ctttgcagaa
     9241 acaactgtca acctatttgg aaaataagga taatgctgcc taccgtttga tgattcgttt
     9301 atccaaagat ggaaagatta gcctctctga tcaacccttg gaacctttgt cagccgattt
     9361 tctgacagct caactctctt tacagaagaa ggacgtgaca gcttcgcctt ttacctactt
     9421 taaaaccagc taccggcctc atattgagca aaaatcttat gaacaggtgt tctataacca
     9481 agcggggcaa ttattggaaa cttccatcgg caatcttttt atccagctgg gtcagaccct
     9541 ctacacacca ccagtggctg tggggatttt accaggactt tttcgccaag agctgctagc
     9601 tactggtcaa gctcaggaaa aagaggtgac actagctgat ttaaaagaag ctagcgctat
     9661 ttttggagga aatgctgtcc gtggcctcta tcctctcaac cttgagctta ctcaccttga
     9721 tgccctctta gctaagagtc aagcctaaac tgccactcat cttttgttat taaggaacct
     9781 atgatactct taattgataa ttacgattca tttacctaca acctcgccca atatttaagt
     9841 gaatttgacg agacgattgt cttgtataac caagacccaa acttatatga catggccaaa
     9901 aaagctaacg ctctagtctt ttcacctggt cctggttggc ccaaggaagc caaccaaatg
     9961 ccaaaactca ttcaagactt ttaccaaaca aaacctatct taggagtgtg tctgggacac
    10021 caagctatcg ctgaaactct aggaggaacc ttacgcttgg caaaacgcgt catgcatggg
    10081 agacaaagca ccattgaaac gcaaggccct gctagtcttt ttcgctccct gccacaagag
    10141 atcaccgtca tgcgttacca ttctatcgtt gtggatcagt taccaaaagg ttttagcgta
    10201 actgctagag actgtgacga tcaagaaatc atggcctttg aacaccacac cctgccactt
    10261 tttgggctac aatttcaccc agaaagcatc ggaactcctg atggcatgac catgatcgcc
    10321 aacttcatcg cagccattcc ccgttaatct catcccccaa aagaaaggag gacagttaca
    10381 tgcctgatca cttagcgctc agaatgcgcc ccaaaaccat ttcagaggtc atcggacaaa
    10441 aacaccttgt tggcgaaggc aaaatcatcc gtcgcatggt ggaagcaaat agactatcgt
    10501 ccatgatcct ctatggccct ccaggaatcg gtaaaacttc cattgcttct gctattgctg
    10561 gtactactag atatgccttt cgtactttta atgccactgt tgatagtaaa aaacgtctcc
    10621 aagaaattgc ggaagaagct aaattttctg gtggcttggt attattacta gacgagattc
    10681 atcgccttga caagacaaaa caagattttc ttttaccttt acttgagaat ggcaccatta
    10741 tcatgattgg cgcaaccact gagaacccct tcttttcagt gacacccgct atcagaagca
    10801 gggtccagat ttttgaattg gaaccacttt ctaacgagga tattaaaaca gccattcagc
    10861 tagcaatttc cgacaaagaa cggggatttc cttttttagt gacaattgat gatgaggcgc
    10921 tagatttcat tgttaccgcc acaaatggag acctacgatc tgcttataat tccttggatc
    10981 tagcagtcat gtctacttct cctaatgaag atagtagccg tcatattagt ctagagacaa
    11041 tggaaaatag tctgcagcgt agttatatta ccatggataa aaacggggac gggcattatg
    11101 atgttttatc tgccttacaa aaatccatta gaggttctga tgtcaatgct agcctgcact
    11161 acgccgctcg tttagtagaa gctggagact tgcctagttt agcaagacga ttgaccatta
    11221 ttgcttacga agatattggc ttagccaacc ctgacgccca agttcatacc gtcaccgcac
    11281 tagatgccgc ccaacgcatt ggttttccag aagctcgtat tcttatcgct aacgtggtca
    11341 ttgatttggc tctatctcct aaatcaaatt cagcttattt agctatggat gcagctttag
    11401 ctgatttgaa aacatcaggt aacctcccta ttccaaggca tcttagagac gggcattatg
    11461 ctggcagcaa ggacctaggc aatgccaaag attacctcta cccacacgcc taccctgaaa
    11521 aatgggtcaa acaacaatac ctaccagata aattagtagg acatcattat tttgaagcca
    11581 atgaaaccgg taaatacgaa cgcgcacttg gttcaaataa agagcgtatt gataagctct
    11641 cagactgact gctagtttta aaaatcgcaa ccgatttcaa aaatcgataa ttttgcttga
    11701 aaaaatttta aaaagtggta ttataaaagt acagaaacat tgctgtggca tacgaatcca
    11761 catctaagtg ttgaccgact atttctttgt attttcggga aacaaagact ctttctagtc
    11821 tgcaagccgt ttcacgcgga cgcaacttcc aagaaagagc gcgttaccca cctgcttata
    11881 ctgcgcgggc tcaatacaaa aacgtggatc accggtgcca atacagcatt cttgcctcct
    11941 tagctcagtt ggtagagcag tagactctta atctatgggt cacaggttcg agccctgtag
    12001 ggggtataca actatacatg aaaaaacctt tgtttaaagg ctttttttgc ttgtcagaag
    12061 aggatttgtt ccatcattta tccccgcagt aattttttta tggtatgata ccaattaaag
    12121 cgcttaagat taaggtggag actgttatga aaataattga gtacaaagga aattacagag
    12181 ataacctcat ttttatggtc ttggaagcaa aaaatgcctt aaggagaatt cccagcatta
    12241 acccggatct tttgacaatt gaaacaagtt atttggaaaa aggggattgt ttttggctag
    12301 cggtcaatga agaaagtcgt gttattggct caatcgctta ttcttctatt cccaaaacaa
    12361 atgaggtctt cttgcaccgt ctcttcatca aaccaagctt aaaacatcaa ggcattggca
    12421 gtcaacttct gacgtttgca gaagattatc taaggaaaat cggcaagagt catgtccgta
    12481 tccacttagg tcaacctaaa gaacaatggt ttgagtctta tcggttttat cctaaacatg
    12541 gttatcaatt ttatgatgac acgcatctca tcaagaaact aatctaaaca catttcctac
    12601 cctttatagt catttcatac gtcattttta ggaggtatcc attgaaaatt gccatcattg
    12661 gacatagtgg ctctggcaag tcaaccttag cacgattttt aggtcaacat taccattgtg
    12721 aggtggtcca tttagaccaa ctacacttct catcgaactg gcaagaacgg tcagatcatg
    12781 acatgatagc tgatttatca acatgcctgc tcaatcaaga ttggattatt gaggggaatt
    12841 atgccaattg tctctatgaa gagcgcatga gtgaggctaa tcacattatc tatgttaatt
    12901 tcagtcggtt tcattgtctc tatcgagcgt ttaagcgtta tttaaactat aggggaaaaa
    12961 cacgtcctga tatggctgac aactgtcagg aaaaatttga cgttgcgttt gtgaaatgga
    13021 ttctactaga tggacgttca agaaaccaat taaaaaaata ccaatccgtt atccaaaaat
    13081 accctcataa aaccattgtc ttaacaaatc aaaagcagct caatcactat atgaatacaa
    13141 tcaactaatt agagtgaaaa actaaaatag ccacgggtaa cccgggctat tcagactgaa
    13201 agcctttgtt taaaggcttt tttgcttgtt tcataactaa tcggcttaat taaaagcgtc
    13261 aatccacttt tagtatcatg tctcaatttt catttg
//
LOCUS       DATURV010000043        13026 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DATURV010000043 DATURV010000000
VERSION     DATURV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13026
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.043"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..335
                     /locus_tag="VCF75_001210"
     CDS             <1..335
                     /locus_tag="VCF75_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP2546772.1"
                     /translation="PQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIVED
                     TRPKLVFHFDNNEPKVEENREKPTKNITPILPATGDIGNVLAFLGILILSVLSIFSLL
                     KNKQNNKV"
     gene            623..1471
                     /locus_tag="VCF75_001211"
     CDS             623..1471
                     /locus_tag="VCF75_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEP2546773.1"
                     /translation="MTERLKNLGILLLFLLGTAIFLYPTLSSQWNAYRDRQLLSTYHK
                     QVTQKKPSEMEEVWQKAKAYNARLGIQPVPDVFSFRDGIHDKNYESLLQIENNDIMGY
                     VEVPSIKVTLPIYHYTTDEVLTKGAGHLVGSALPVGGDGTHTVISAHRGLPSAEMFTN
                     LNLVKRGDTFYFRVLNKVLAYKVDQILTVEPDQVTSLSEVMGKDYATLVTCTQYGVNT
                     KRLLVRGHRIAFHYKKYQQAKKAMKLVDKSRMWTEVVCAAFGVVIAIILVFMYSKMTD
                     KKVIKN"
     gene            1757..4018
                     /locus_tag="VCF75_001212"
     CDS             1757..4018
                     /locus_tag="VCF75_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEP2546774.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMLLVLIGFEGKV
                     RAAHELVEVPVPIFHNPDPQSDYQWYGYEAYTGGYPKYDLFKTYYHDLRVNLHGSKSY
                     QVYCFNVHKHYPRPSQSFDRKWYKKLDGTAENFDSLAMEPRVRKEELTKKLRAVMYNA
                     YPNDANGIMKDLEPLNAIKVTQEAVWYYSDNSPINPDESFKSEALANKISATQISLMR
                     EALKQLIAPDLDEKQSRRVPDGYQLSIFESENKDYQNLLAAEEVPIQPPKPGDKPPLP
                     PVQTKQTSVIIRKYAEGDYSKLLEGATLKLAQIEGSGFQEKIFDSNKSGEKVELPNGT
                     YVLSELKPPQGYGVATPITFKVAAGKVLIKNKEGQFVENQNKEIAEPYSVTAFNDFEE
                     IGYLSDFNNYGKFYYAKNTNGTNQVVYCFNADLHSPPDSYDHGANIDPDVSESKEIKY
                     THVSGYDLYKYAVTPRDKDADLFLKHIKKILDKGYKKKGDTYKTLTEAQFRAATQLAI
                     YYYTDSADLTTLKTYNDNKGYHGFDKLDDATLAVVHELITYAEDVTLPMTQNLDFFVP
                     NSSRYQALIGTQYHPNELIDVISMEDKQAPIIPITHKLTISKTVTGTIADKKKEFNFE
                     IHLKSSDGQAISGTYPTNSGELTVTDGKATFTLKDGESLIVEGLPSGYSYEITETGAS
                     DYEVSVNGQNVPDGKVMKELVKEDETVAFENRKDLVPPTGLTTDGAIYLWLLLLVPFG
                     LLVWLFGRKGTKK"
     gene            4015..4536
                     /gene="lepB"
                     /locus_tag="VCF75_001213"
     CDS             4015..4536
                     /gene="lepB"
                     /locus_tag="VCF75_001213"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP2546775.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYIFGVMII
                     NTNDMSPALSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVSFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            4558..5607
                     /locus_tag="VCF75_001214"
     CDS             4558..5607
                     /locus_tag="VCF75_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEP2546776.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKAETAGVVSSGQLTIKKS
                     ITNFNDDTLLMPKATFTFDVKPVDVTGEEKDTASGLKIQKGIAGVASQTIQYDNTDKP
                     TNKEKAVNFDFSTVTFPNVGVYRYEVSEQAGDVKGITYDSKKWTVDVYVVNENNKFTP
                     KYIVSKETTDSSKKPIVFNNELKTTSLTIKKEVTGNSGDKTSDFTFTLLLKENTQFET
                     GQKVKATKKKSGAEEETVEVTIGKQYTFTLKDSEELILDKLPIGITYQVDETDKNKEQ
                     YETSAKMTEDSKEAQGYTLNDLKTTDETADTITVTNKRNLPIPTGVVGILAPFAVLSI
                     VAIGGVIYITKRKKA"
     gene            5623..6348
                     /gene="srtB"
                     /locus_tag="VCF75_001215"
     CDS             5623..6348
                     /gene="srtB"
                     /locus_tag="VCF75_001215"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEP2546777.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNDFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            6365..6952
                     /locus_tag="VCF75_001216"
     CDS             6365..6952
                     /locus_tag="VCF75_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEP2546778.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSVALESIDAMKTIEEITIAGSGKASFSSLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDGTLVAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPDIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSKL"
     gene            complement(7111..8316)
                     /locus_tag="VCF75_001217"
     CDS             complement(7111..8316)
                     /locus_tag="VCF75_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEP2546779.1"
                     /translation="MVIFDLKHVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQATQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNISLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            8707..12084
                     /locus_tag="VCF75_001218"
     CDS             8707..12084
                     /locus_tag="VCF75_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HEP2546780.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGSFEIKKVDQNNKPLPGATFSLTPKDGKGKPGQTFTSNDKGIVDAQNLQPGTYTLK
                     EETAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLEKSTMSV
                     VSKYGEQEKTSNLADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDQRLNPKGI
                     SQDIPKIIYDSANSPLAIGKYDADSHQLIYTFTDYIAGLDKVRLSAELSLFLENKEVL
                     ENTNISDFKSTIGGQEITYKGTVNVIYGNKSGDSNNYVKNILSNVGGSIESYNTQTGD
                     FTWYVYVNPKKQSINYSILNLWGFGRARSNTNNLETNANTSSAQLEKVEIYEVKEDYT
                     LPTSYGVDITKLTLRTDITVGNKDGFPMTKRQRIDFKDNLKDGKRFVVKVTGKTDQSG
                     KPLVVQSNLASFSTNQDYVDLKISGGNVYFQNEIALSPSKGSGSGKSEFTKPSITVAN
                     LKRVAQLRFKKMSTDNVPLPEATFELRSSTSDSQKVEAKSNEQGEVHFKDLNSGTYDL
                     YEKQAPKGYQQVTEKLATVTVDTTKPAEEMVKWEKTHPFVKVEVNKEVTIVNHKETLT
                     FSGKKIWENDRPDQRPAKIQVQLLQNRQKMSDKVQEVTKDNDWSYHFKDLPKYDDKNQ
                     EYKYSVEEVKVPDGYKVSYLRNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKIID
                     EDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKNLK
                     KYNGTGNDIMYSVKEVTVPTGYDVTYSANDIINTKREVITQQGPNLEIEEMLPLESGA
                     SGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATMEL
                     RDSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQVTVNG
                     KATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDVIIGGQG
                     QIVETTEDTQTGMHGDSGCKTEVEDTKLVQSFHFDNKESESNSEIPKKDKPKSNTSLP
                     ATGEKQHNMFFWMVTSCSLISSVFVISLKTKKRLSSC"
     gene            complement(12350..13015)
                     /locus_tag="VCF75_001219"
     CDS             complement(12350..13015)
                     /locus_tag="VCF75_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546781.1"
                     /translation="MKRCNKYLFTSLLAVSILFSSYKSVHAHDNIDEKGKVHLYWQGN
                     YYVDNYVDYTKTLVDNNNSIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPKEITYEHHY
                     WDGTVDSKTRSNTQWHYDWENQKTNFNQEFDKFPGYTGWSPSLDKFYKLKNDGKFSHV
                     LVDTYGRQSHTYFSHKMVWKFKTELEEGYKDKWNKLPFIAGIKQNNPLAASFPSYKGE
                     FGE"
BASE COUNT         4488 a         2109 c         2583 g         3846 t
ORIGIN      
        1 ctcctcaggt tgagacagaa gatacgaaag agccaggagt gttgatggga ggtcaaagtg
       61 agtctgttga atttactaaa gacactcaaa caggcatgag cggtttcagt gaaacagtga
      121 ccattgttga agatacgcgt ccgaagttag tgttccattt tgacaataat gagcccaaag
      181 tggaagagaa tcgggaaaag cctacaaaaa atataacacc tatccttcct gcaacaggag
      241 atattggaaa tgttttggcc tttcttggaa tccttatttt gtcagtactt tctattttta
      301 gccttttaaa aaacaaacaa aacaataaag tctgacgtaa aagtgttcca taagaaataa
      361 tgcgcaaata tgctgaatag tggataactg ctacagtcaa gacaagtgtt aattcaaaag
      421 gtactactta tacatctgat aataataagg tgacagataa ttcagagcaa gtaggatggc
      481 taaaaaatga tgtattctat gtataataga cttacaagaa atcaagttat tgttaaacgt
      541 cgtaaagctt aacgctttca aaagtcgaaa tcaatcgaga ctgtctttag gcagtctcga
      601 tttttattga taaggaactg caatgacaga aagactaaaa aatctaggga tactcttatt
      661 atttttattg ggaacagcca tttttcttta ccctacgcta agtagtcagt ggaatgccta
      721 tcgtgatcgt caattgttat caacttatca caaacaagtg actcaaaaga agccctcaga
      781 aatggaggag gtgtggcaaa aggctaaggc gtataacgct aggcttggga ttcagcccgt
      841 tccagatgtt ttttcattta gggatggcat tcatgacaaa aattacgaaa gcctacttca
      901 aattgaaaac aatgatatta tgggatatgt tgaagtgccc tctattaaag tgacgttacc
      961 catataccac tatacgacag acgaggtgct aactaaggga gcaggtcact tagttggaag
     1021 tgccttacca gttggtggcg acgggactca cacggttatt tctgctcata gaggccttcc
     1081 atcggcagaa atgtttacta atttgaattt agtgaaaagg ggagatactt tttactttcg
     1141 tgtgttaaat aaagtattgg cttataaagt tgatcagata ctaactgttg aaccagatca
     1201 agtcacatct ctttctgagg taatgggaaa agattatgct actctggtta cctgcacaca
     1261 atatggtgtt aataccaagc gtctattggt cagaggtcat agaattgcat ttcattacaa
     1321 aaagtatcaa caggctaaaa aagcaatgaa actcgtggat aaatcaagaa tgtggacaga
     1381 agtagtatgt gctgctttcg gggtggttat agctattatt ttagtattta tgtattcaaa
     1441 gatgactgat aaaaaagtga ttaaaaacta acttgccctg aatcttcatt atcgtactga
     1501 aactgtcgta aaagtaaaat aatctggtag ttactatccc ctttgatgaa gtttaattaa
     1561 atctcatcaa gagaaaaata ttaggcggtt acaaatgatt ttcccaaaaa ttattttttg
     1621 gaacgcataa tatggaaaaa aagatgataa gaacaactgt tttaaacatc aaataaatga
     1681 tatagtagct atgaaatagc attgtgactg tccggtaaag ggatgggact gaagatttta
     1741 ttcagaagga gagggaatga ataacaaaaa attgcaaaag aagcaagatg ctcctcgggt
     1801 atcaaacaga aagccaaaac aattaactgt cactttagtg ggagtatttt taatgctctt
     1861 agtattgata ggatttgaag gaaaagttcg agcagctcac gaattggttg aggtaccagt
     1921 acctattttt cacaatccag atccacagtc agactatcag tggtatggct atgaagccta
     1981 tacagggggg tatccgaagt atgatttatt caagacatac tatcatgatc tgagagtgaa
     2041 tttgcatggg agtaaaagtt atcaagtata ttgttttaac gttcacaaac actatcctcg
     2101 tccctcccag agttttgatc ggaagtggta taaaaaatta gatggaactg ctgagaattt
     2161 tgattctctt gctatggaac caagagttcg taaagaggag ttaaccaaaa aactccgagc
     2221 tgttatgtat aatgcctatc ctaatgatgc taatggcatt atgaaagatt tagagccttt
     2281 aaatgctatt aaagtcactc aagaagctgt atggtactat tcggacaatt ctcctattaa
     2341 cccagatgaa agttttaaat ctgaagctct ggctaataaa attagtgcta cacaaatatc
     2401 tttaatgaga gaggctttga agcaacttat tgctcctgat ttagatgaga agcaatctag
     2461 gagagttcct gatggatatc agttgagtat ttttgaatct gagaataagg attatcaaaa
     2521 tcttttggct gcagaagagg ttcctattca acctccaaaa ccgggggata agccaccact
     2581 accgccagtg caaacaaaac aaacatcagt cattatcaga aaatatgcgg aaggtgacta
     2641 ctctaaactt ctagagggag caaccttaaa gcttgctcaa attgaaggaa gtggttttca
     2701 ggagaaaatt tttgatagta acaagtctgg agagaaagta gagctaccta atgggactta
     2761 cgttttaagt gagctaaaac ctcctcaagg atatggagtt gccacaccca ttacttttaa
     2821 agttgcagct gggaaagttt taataaaaaa taaagaaggt caatttgtag agaaccaaaa
     2881 taaggaaatc gcagagccat attctgtaac agctttcaat gactttgaag agattggtta
     2941 tttatctgat tttaataact atggtaagtt ttactatgca aaaaatacta atggaactaa
     3001 tcaagttgtc tactgtttca atgctgattt acactcacca cctgactcat atgatcacgg
     3061 agcaaatatt gatcctgatg ttagtgaaag taaagagata aagtatacac atgtttctgg
     3121 ctatgatttg tataaatatg cagtcacacc aagagacaaa gatgctgatt tattcttaaa
     3181 acatatcaag aaaattcttg ataaaggtta taagaagaag ggggatacct ataagacatt
     3241 aactgaagct cagtttagag cagcaactca attagctatt tattactata cagatagtgc
     3301 tgatttaaca acacttaaga catataatga taacaagggt tatcatggtt ttgataaact
     3361 ggatgatgca acattagctg tggttcatga gttgatcacc tatgctgagg atgtcacctt
     3421 accaatgact caaaatctag atttctttgt tcctaatagc agtagatacc aggcacttat
     3481 tggaacgcag tatcatccaa atgaattgat tgatgttatt tctatggaag ataaacaggc
     3541 tcctattatt cctattactc acaagttaac tatttctaaa actgttactg gaactattgc
     3601 agataagaaa aaagaattta actttgaaat acatttaaaa tcttctgatg gacaagctat
     3661 aagtggaaca tatccgacaa actctggaga actcacagtt acagatggaa aagctacctt
     3721 cacattaaag gatggagaat cattgattgt tgaggggcta ccttcaggtt actcttatga
     3781 aattaccgaa acgggtgctt cagattatga ggtaagtgtt aatgggcaga atgtacctga
     3841 tggaaaagtg atgaaagaac tagttaagga agatgagact gtagcttttg aaaaccgaaa
     3901 agatcttgtc ccaccaactg gtttgacaac agatggggct atctatcttt ggttgttatt
     3961 acttgttcca tttgggttat tggtttggct atttggtcgt aaagggacta aaaaatgact
     4021 aattacctaa atcgcttaaa tgagaatcca ctatttaaag ctttcatacg gttagtactt
     4081 aagatttcta ttattggatt tctaggttac attctatttc agtatatttt tggtgtcatg
     4141 attattaaca ctaatgatat gagtcctgct ttaagtgcag gtgacggtgt tttatattat
     4201 cgtttgactg atcgctatca tattaatgat gtggtggtct atgaggttga taacactttg
     4261 aaagttgggc gaattgccgc tcaagctggc gatgaggtta gttttacgca agaaggagga
     4321 ctgttgatta atgggcatcc accagaaaaa gaggtccctt acctgacgta tcctcactca
     4381 agtggtccaa actttcccta taaagttcct acgggtacgt atttcatatt gaatgattat
     4441 cgtgaagaac gtttggacag tcgttattat ggggcgttgc ccatcaatca aatcaaaggg
     4501 aaaatctcaa ctctattaag agtgagagga atttaaaaaa atttggagag agaaaaaatg
     4561 aaaaaaaaca aattattact tgctactgca atcttagcaa ctgctttagg aacagcttct
     4621 ttaaatcaaa acgtaaaagc tgagacggca ggggttgtta gcagtggtca attaacaata
     4681 aaaaaatcaa ttacaaattt taatgatgat acacttttga tgcctaaggc tacatttacc
     4741 tttgacgtta aaccggttga tgtaacagga gaagaaaagg atacagcttc aggcttaaag
     4801 attcaaaaag gtattgctgg agtagcatcg cagacaatcc aatacgataa tactgataag
     4861 ccaacgaaca aagagaaggc agtaaacttt gatttttcta cagtaacttt tccaaacgta
     4921 ggggtttacc gatatgaagt ttcagaacaa gctggtgacg tgaaggggat tacttatgat
     4981 tctaaaaagt ggacagtaga tgtctatgta gtaaatgaaa ataacaagtt cacacctaaa
     5041 tatatcgtct caaaagaaac cacagactca agcaaaaaac cgattgtctt taataatgag
     5101 ttaaaaacaa cttctttaac tatcaaaaaa gaagttacgg gaaacagtgg agataaaaca
     5161 agtgatttta catttactct cctcttgaaa gaaaatactc agtttgaaac aggtcagaaa
     5221 gtcaaggcta ctaagaagaa gtctggtgct gaagaagaaa cagtagaagt cacaattggt
     5281 aagcaatata cctttacttt gaaagatagt gaggagctga tactagataa attaccgatt
     5341 ggtattacct atcaggtaga tgagactgat aaaaataagg aacagtatga aacatccgca
     5401 aagatgacag aagattcgaa agaagcacaa ggctatacgt taaatgattt aaaaacaaca
     5461 gatgagactg ctgatacaat cactgttacc aataagagaa acttgcctat cccaactggt
     5521 gttgtaggca tccttgctcc atttgcagtt cttagcattg tggctattgg tggagttatc
     5581 tatattacaa aacgtaaaaa agcttaagtg attagtcaaa gaatgatgat gacaattgta
     5641 caggttatca ataaagccat tgatactctc attcttatct tttgtttagt cgtactattt
     5701 ttagctggtt ttggtttgtg ggattcttat catctctatc aacaagcaga cgcttctaat
     5761 ttcaaaaaat ttaaaacagc tcaacaacag cctaaatttg aagacttgtt agctttgaat
     5821 gaggatgtca ttggttggtt aaatatccca gggactcata ttgattatcc tctagttcag
     5881 ggaaaaacga atttagagta tattaataaa gcagttgatg gcagtgttgc catgtctggt
     5941 agtttatttt tagatacacg gaatcataat gattttacgg acgattactc tctgatttat
     6001 ggccatcata tggcaggtaa tgccatgttt ggcgaaattc caaaattttt aaaaaaggat
     6061 tttttcaaca aacataataa agctatcatt gaaacaaaag agagaaaaaa actaaccgtc
     6121 actatttttg cttgtctcaa gacagatgcc tttgaccagt tagtttttaa tcctaatgct
     6181 attaccaatc aagaccaaca aaggcagctc gttgattata tcagtaaaag atcaaaacaa
     6241 tttaaacctg ttaaattgaa gcatcataca aagttcgttg ctttttcaac gtgtgaaaat
     6301 ttttctactg acaatcgtgt tatcgttgtc ggtactattc aagaataacg aaaggaggag
     6361 acttttgaga aaatattgga aaatgttatt ttctgtcgta atgatattaa ccatgctggc
     6421 ctttaatcag actgttttag caaaagacag cactgttcaa actagcatta gtgtcgaaaa
     6481 tgtcttagag agagcaggcg atagtacccc attttcggtt gcattagaat caattgatgc
     6541 gatgaaaaca atagaagaaa taacaattgc tggttctgga aaagcaagct tttcctctct
     6601 gaccttcaca acagttgggc aatatactta tcgtgtttat cagaagcctt cacaaaataa
     6661 agattatcaa gcagatacta ctgtatttga cgttcttgtc tatgtgacct atgatgaaga
     6721 tgggactcta gtcgcaaaag ttatttctcg aagggctgga gacgaagaaa aatcagcgat
     6781 tacttttaag cccaaacggt tagtaaaacc aataccgcct agacaacctg acatccctaa
     6841 aaccccatta ccattagctg gtgaagtaaa aagtttattg ggtatcttaa gtatcgtatt
     6901 actggggtta ctagttcttc tttatgttaa aaaactgaag agtaagctat aaaacccatt
     6961 tttaagagtc acacattttt agttgctcta tttttataat gaaacttatt gacatctcca
     7021 tagtcttgct tgactttgga gatgttttct atgctgatag gtttttgtac catataagac
     7081 aatgattgat gaaaagagtg tcttgaattc ttaaatatta tctcggtatt tagcaagata
     7141 gtcttttgaa gaaatacccg tgtatttttt aaagatttta tgaaaatggg tagtgtcata
     7201 aaaatataag cttttagcca cttctccaac aggaattcct cgttttaaga gtagttgagc
     7261 ttctttgatt tttgtactta gaatataatg ttgtaaggaa atatttgagt attttttaaa
     7321 gactgaacgt aagtgagatt cggaaacata taggcgctta gcgatatcag atacctttaa
     7381 tttggaatac aggtgactat cgacataatg aagcacacta ttgtacattt gacgtttatc
     7441 agaaatactt ttattggtta atgattcgga aaaatggatg atagctgcac agcgaattct
     7501 caaaactttg agcaagtcac tcgcttcttc atttaaggcc atgtagtatt tgacaaggct
     7561 ataatgcttt gctacgtcag tattttctac aaaactgaat tccagtaacc tagttaaata
     7621 gataacagtg aggttctttt ccgcccttag agcagaaata gaagatgagg tgatacttga
     7681 taaggggata cgattgattt cttgcttgag caattgtgga ttgcctagct ttaccagatc
     7741 cataatatga tttaggtgct ctaaaacagg cggtaattgg ctagatgctt tgctaatatg
     7801 cttaaggcga tgaatgatat caggatcagc agtcatctgt tttgaatgct gcaacaattg
     7861 atgaattgtt tgttggcaag tcgtctcaaa ttgtgtgtca aaaaaagcgt caatagctat
     7921 caaaaggttc cgcacattat taattggaaa atgtgggaca agtgccatat aggataagag
     7981 ctcttctttg ctacgatgag aagaatgttt taaaaaatta ttagctaatt gttcctgata
     8041 gtctttatta agtgaataag ggtagaaagg tccaatggca ataatgtagt gacaaagagg
     8101 aaaaatcata aaggactctt caaataaacc ctcataaaaa ataacatctt gtgtggcttg
     8161 aggaatagct agatgcttta aaaattgata gtaacataat aaatagtcgt cattaccata
     8221 tacttgaata agtgccttat cttgtgacat cactgaaata ggtaattgag acaagctgtg
     8281 taatgtttgc acatgtttta aatcgaatat aaccatttga tagattgccc cccatcctta
     8341 gttgtatcat tttccttaga cttgtaaaag ctttagtaaa tgttaatgat gaaaattcct
     8401 atttggtagg cacactaaaa ggtcttccta atagtaaact ctctctccat catctcctgc
     8461 cttaattata ctatgaatag ctaagcagat aaagataaag gacttgtacg ttaagcgctt
     8521 gaaaaagaaa gagttacaga taatgacata aaaaacgcta aaaaaccatc aaaataaata
     8581 ctctaaccat aagatgtaga cttgacaact gaaaatggta atggtaaaat aactatttga
     8641 cagttgtcct gtagtcttta gttttggaca taggctgtcg cttatgaatg tggagagaga
     8701 aaataaatga cacaaaaaaa tagctataag ttaagcttcc tgttatcctt aacaggattt
     8761 attttaggtt tattattggt ttttatagga ttgtccggag tatcagtagg acatgcggaa
     8821 acaagaaatg gagcaaacaa acaaggatct tttgaaatca agaaagtcga ccaaaacaac
     8881 aagcccttac cgggagcaac tttttcactg acaccaaagg atgggaaggg aaaacctggt
     8941 cagacattca cttcaaatga taaaggtatt gtagatgctc aaaatctcca accagggact
     9001 tataccttaa aagaagaaac agcaccagat ggttatgata aaaccagtcg gacttggaca
     9061 gtgactgttt atgagaacgg ctataccaag ttggttgaaa atccctataa tggggaaatc
     9121 atcagtaaag cagggtcaaa agatgttagt agttctttac agttagaaaa atcaacaatg
     9181 tctgttgtat ccaagtacgg tgaacaggaa aaaacatcaa acttagcgga tttctaccgt
     9241 aaccatgccg cctattttaa aatgtctttt gagttgaaac aaaaggataa atctgaaaca
     9301 atcaacccag gcgatacctt tgtgttacag ctggaccaac gtctcaatcc taaaggtatc
     9361 agtcaagata tccctaaaat catttatgac agtgcaaata gtccgcttgc gattggaaaa
     9421 tacgatgccg atagccatca acttatctat actttcacag attatattgc aggtttagat
     9481 aaagtccggt tgtctgcaga attgagctta ttcctagaga ataaggaagt gttagaaaac
     9541 accaacattt cagattttaa gagtaccata ggtgggcagg agatcaccta taaaggaacg
     9601 gtcaatgtta tctatgggaa taaatcggga gacagcaata attatgttaa gaatatattg
     9661 agcaatgtgg gtggaagtat tgaaagctac aatacacaaa caggcgactt tacttggtat
     9721 gtttatgtca atcctaaaaa gcaatctatc aactattcta tcctaaattt atggggattt
     9781 ggaagggctc gttcaaacac aaacaactta gaaacaaatg ctaatacaag tagtgcccaa
     9841 ttggaaaaag tagaaattta tgaagttaaa gaggactata cgctacctac tagctatggg
     9901 gttgatatta caaaacttac tttaagaacg gatatcacag tggggaataa agatggtttt
     9961 ccaatgacta aacgtcagcg gattgatttt aaagataatc ttaaagatgg aaaaaggttt
    10021 gttgttaaag taacagggaa aacagaccaa tctggtaagc cactagtcgt tcaatccaat
    10081 ttagccagtt tttcaactaa tcaagattat gttgacttaa aaataagtgg agggaatgtc
    10141 tacttccaaa acgaaattgc cttgtctcct tctaagggta gtggttctgg gaaaagtgaa
    10201 tttactaagc cctctattac agtagcaaat ctaaaacgag tggctcagct tcgctttaag
    10261 aaaatgtcaa ctgacaatgt gccattgcca gaagcgactt ttgagctgcg ctcatcaact
    10321 agtgacagtc aaaaagtaga agcaaaatca aatgaacaag gagaggttca ctttaaggac
    10381 ctgaattcgg gcacctatga cttgtatgaa aaacaagcac ctaaaggcta tcagcaggtg
    10441 acagagaaat tggcgaccgt tactgttgat actaccaaac ctgctgagga aatggtcaag
    10501 tgggaaaaga cacatccgtt tgtaaaagta gaagttaaca aagaagtcac gattgtcaac
    10561 cataaagaaa cccttacgtt ttcagggaag aaaatttggg agaatgacag accagatcaa
    10621 cgcccagcaa agattcaagt gcaactgttg caaaatcgtc aaaaaatgtc cgataaagtt
    10681 caagaagtaa cgaaggataa cgattggtct tatcacttca aagacttgcc taagtacgat
    10741 gacaagaatc aggagtataa gtactcagtt gaagaagtaa aagttccaga cggctacaag
    10801 gtgtcgtatt taagaaatga tatatttaac accagagaaa cagaatttgt gtttgaacag
    10861 aataacttta accttgaatt tggaaatgct gaaataaaag gtcaatctgg gtcaaaaatc
    10921 attgatgaag acacgctaac gtctttcaaa ggtaagaaaa tttggaaaaa tgatacggca
    10981 gaaaatcgtc cccaagccat tcaagtgcag ctttatgctg atggagtggc tgtggaaggt
    11041 caaaccaaat ttatttctgg ctcaggtaat gagtggtcat ttgagtttaa aaacttgaag
    11101 aagtataatg gaacaggtaa tgacatcatg tactctgtta aagaagtaac tgttccaaca
    11161 ggttatgatg tgacttactc agctaatgat attattaata ccaaacgtga ggttattaca
    11221 caacaaggac cgaacctaga gattgaagaa atgcttccgc tagaatcagg tgcttcaggt
    11281 ggtaccacta ctgtcgaaga ctcacgccca gttgatacct tgtcaggttt atcaagtgag
    11341 caaggtcagt ccggtgatat gacaattgaa gaagatagtg ctacccatat taaattctca
    11401 aaacgtgata ttgacggcaa agagttagct ggtgcaacta tggagttgcg tgattcatct
    11461 ggtaaaacta ttagtacatg gatttcagat ggacaagtga aagatttcta cctgatgcca
    11521 ggaaaatata catttgtcga aaccgcagca ccagacggtt atgagatagc aactgctatt
    11581 acctttacag ttaatgagca aggtcaggtt actgtaaatg gcaaagcaac taaaggtgac
    11641 gctcatattg tcatggttga tgcttacaag ccaactaagg gttcaggtca ggttattgat
    11701 attgaagaaa agcttccaga cgagcagggc cattctggct caactactga aatagaagat
    11761 agcaagtctt cagacgttat cattggtggt caggggcaga ttgtcgagac aacagaggat
    11821 acccaaactg gcatgcacgg ggattctggt tgtaaaacgg aagtcgaaga tactaaacta
    11881 gtacaatcct tccactttga taacaaggaa tcagaaagta actctgagat tcctaaaaaa
    11941 gataagccaa agagtaatac tagtttacca gcaactggtg agaagcaaca taatatgttc
    12001 ttttggatgg ttacttcttg ctcacttatt agtagtgttt ttgtaatatc actaaaaact
    12061 aaaaaacgcc tatcatcatg ttaagataag ctgttggtga caatagcgaa aaagggtcca
    12121 cttgttagtg gacctctttt tctggaatcg atcgtctcgg ttcactagat aagtcatttt
    12181 aagtagtcaa aataggcaga gccttaaaag gttttctgcc tttttaatct tgttgggcac
    12241 ttacgaaagt atttcggcca cttcaagcat aataaaaaga gtctgagcaa tcatgttgtc
    12301 tcagactctg actgtaatta tgcaaaagat gatggactgg cagcttcatt tactcaccga
    12361 actccccttt ataacttggg aatgatgctg ctaatgggtt attttgttta atacctgcta
    12421 taaacggtaa tttattccat ttatctttgt aaccttcttc aagctcagtt ttaaacttcc
    12481 aaaccatttt atgagaaaaa taagtgtgtg attgacggcc gtaggtgtct actaaaacgt
    12541 gagaaaattt tccgtcgttt tttagtttgt aaaatttatc tagagaagga ctccaaccag
    12601 tataaccagg gaatttatca aattcttgat taaaatttgt tttttggttt tcccaatcat
    12661 aatgccattg tgtattactg cgtgttttag aatcaactgt accgtcccaa taatgatgct
    12721 cataagttat ctccttagga gcttttacac ccttaggaag aaacactgag aaatcagggt
    12781 aaacccattg ttctttagct gaattaaagg ttacagtcca ttcgatactg ttattgttat
    12841 cgaccaatgt tttagtatag tcgacatagt tatcaacata gtaatttccc tgccagtaaa
    12901 ggtgcacttt acctttctca tcaatgttat catgggcatg tactgattta tatgaactaa
    12961 acagaatcga aactgctagt agtgaagtga agagatattt attacatcgt ttcatatcat
    13021 gaaaaa
//
LOCUS       DATURV010000044        13024 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DATURV010000044 DATURV010000000
VERSION     DATURV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.044"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            126..551
                     /gene="rplK"
                     /locus_tag="VCF75_001220"
     CDS             126..551
                     /gene="rplK"
                     /locus_tag="VCF75_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEP2546782.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            657..1346
                     /gene="rplA"
                     /locus_tag="VCF75_001221"
     CDS             657..1346
                     /gene="rplA"
                     /locus_tag="VCF75_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEP2546783.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            1669..2397
                     /locus_tag="VCF75_001222"
     CDS             1669..2397
                     /locus_tag="VCF75_001222"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEP2546784.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            2426..2983
                     /gene="frr"
                     /locus_tag="VCF75_001223"
     CDS             2426..2983
                     /gene="frr"
                     /locus_tag="VCF75_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEP2546785.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            3092..3949
                     /locus_tag="VCF75_001224"
     CDS             3092..3949
                     /locus_tag="VCF75_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP2546786.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            4022..4531
                     /gene="msrA"
                     /locus_tag="VCF75_001225"
     CDS             4022..4531
                     /gene="msrA"
                     /locus_tag="VCF75_001225"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEP2546787.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPKQIAYKDLVELYWAQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            4528..4743
                     /locus_tag="VCF75_001226"
     CDS             4528..4743
                     /locus_tag="VCF75_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEP2546788.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            4899..6068
                     /locus_tag="VCF75_001227"
     CDS             4899..6068
                     /locus_tag="VCF75_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP2546789.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQVSATSQSTVPMAPSATPSDVPTTPFASAKPDSSVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEVSSAAPAQAPAEKEETSAPAAQKAVADTTSVATSNGLSYAPNHAYNPMN
                     AGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQVAQYA
                     IDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFNA"
     gene            6382..8154
                     /locus_tag="VCF75_001228"
     CDS             6382..8154
                     /locus_tag="VCF75_001228"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEP2546790.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEGKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            8313..9365
                     /locus_tag="VCF75_001229"
     CDS             8313..9365
                     /locus_tag="VCF75_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEP2546791.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            9411..9986
                     /locus_tag="VCF75_001230"
     CDS             9411..9986
                     /locus_tag="VCF75_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEP2546792.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            10145..10642
                     /gene="ybeY"
                     /locus_tag="VCF75_001231"
     CDS             10145..10642
                     /gene="ybeY"
                     /locus_tag="VCF75_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEP2546793.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            10623..11030
                     /locus_tag="VCF75_001232"
     CDS             10623..11030
                     /locus_tag="VCF75_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEP2546794.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            11150..12046
                     /gene="era"
                     /locus_tag="VCF75_001233"
     CDS             11150..12046
                     /gene="era"
                     /locus_tag="VCF75_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEP2546795.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            12067..12543
                     /locus_tag="VCF75_001234"
     CDS             12067..12543
                     /locus_tag="VCF75_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP2546796.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
BASE COUNT         4064 a         2392 c         2826 g         3742 t
ORIGIN      
        1 tattttaaca aaaagacttg atttattttg gcaataagct ataatagtaa ggtatgcaat
       61 attgcatatc tgtgggaggt aaaaatctca attaccgcca aaaccacaac aggaggattt
      121 ttaaaatggc taaaaaagtc gaaaaacttg taaaacttca aatccctgct ggtaaagcta
      181 caccagctcc accagttgga ccagctcttg gtcaagcagg tatcaacatc atgggcttca
      241 ctaaagaatt taacgctcgt acagctgatc aagctggtat gatcatccca gttgttatct
      301 cagtttatga agacaaatca tttgatttca tcactaaaac accaccagct gctgttcttt
      361 tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taacactact aaggttgcga
      421 cagttactcg tgcacaagta caagaaattg ctgaaactaa gatgccagat ttgaacgctg
      481 caaacattga agctgcaatg cgtatgatcg aaggtactgc tcgttctatg ggattcactg
      541 ttactgacta gtcagtcaag aacccctatt acccgcacga cttcatcata agagatgagt
      601 gacgtgggag attttaaatc aaatcgatat gaccacatta caaggagaat tttaaaatgg
      661 ctaaaaaaag caaacaaatg cgtgctgcac ttgaaaaagt agatagcaca aaagcgtaca
      721 gtgtagaaga agctgtagca ttagtaaaag aaacgaactt cgcaaaattt gatgcgtctg
      781 ttgaagttgc ttacaacttg aacattgacg ttcgtaaagc agaccaacaa atccgtggcg
      841 caatggtatt gccaaacgga actggtaaaa cacaacgtgt tcttgttttt gcacgtggtg
      901 caaaagctga agaagcaaaa gcagctggtg cagacttcgt aggtgaagac gaccttgttg
      961 caaaaatcaa tggtggatgg cttgactttg acgttgttat cgcaacgcca gacatgatgg
     1021 ctatcgtagg tcgtcttgga cgtgtccttg gacctcgtaa cttgatgcca aaccctaaaa
     1081 ctggtacagt aacgatggat gttgctaaag ccgttgaaga gtctaaaggt ggtaaaatca
     1141 cttaccgtgc tgacaaagca ggtaatgttc aagctcttat tggtaaagtt tcatttgatg
     1201 ctgacaaatt ggttgaaaac ttcaaagcct tccacgatgt aatggctaaa gctaagcctg
     1261 caacagctaa aggaacttac atggcaaacg tctcaatcac atcaacacaa ggtgttggta
     1321 tcaaggttga tcctaactca ctttaagaaa aaaattaggg catcgcctaa tcatagcaaa
     1381 aagacaaatt tctgaagaca aatcttcact agaggtttgt cttttttatg gtttaaaatg
     1441 gaatgcaaac aaaaaagtgt aggcgataat accatgaaag gagttgattc tcttaatgag
     1501 atgtggtgtt aacacatcat gagctcacaa aaaatgataa aggaagtatc ggtggtgtta
     1561 aaaaaaggga ttttaattga tatttattag cctctaaaat gtcctttccc tattcataat
     1621 tgagggtttg tggtaaaata gtaaggacaa actaaggaga acatagtagt ggaacctaaa
     1681 tatcaacgta tattaattaa gttatctggt gaagcattag cgggtgaaaa aggagttggc
     1741 attgacattc caactgttca ggctattgca aaagaaattg ccgaagttca tgtgtcggga
     1801 gtacagatcg ctcttgttat tggtggaggc aatctctggc gtggagagcc tgcagcagac
     1861 gctggaatgg accgtgttca ggctgattac actggcatgc ttggaacggt gatgaatgcc
     1921 ctagtcatgg ctgatagtct tcaacattat ggtgttgata cccgtgtgca aacagctatt
     1981 cctatgcaaa atgtagcaga accttatatt cgcggacgtg ccttgcgcca tctagaaaaa
     2041 aatcggattg ttgtttttgg agctggtatt ggctcgcctt atttttcaac agacaccacc
     2101 gcggcactgc gtgctgctga gattgaagca gatgctattt taatggcaaa aaatggtgtg
     2161 gatggggtct ataatgctga tcctaaaaaa gatgctaatg cggttaaatt tgatgaatta
     2221 acacatggtg aagtgatcaa acgtggtctc aaaatcatgg atgcaacggc atcgacctta
     2281 tcgatggata acgatattga tttggtggtt ttcaatatga atgaagctgg caatattcaa
     2341 cgtgttgtct ttggagaaca tattggaact actgtatcaa ataaagtttg tgactaaaag
     2401 ttagaaaaga aagagagtaa ttatcatggc aaatgcaatt attgaaactg caaaagaacg
     2461 ttttgcacaa tcccatcagt ctttatcacg tgaatatgcc agcattcgtg cgggtcgtgc
     2521 caacgctagt cttttagacc gtatccaagt tgattattac ggggcaccga cgccattaaa
     2581 tcaattggct tcaatcactg tgccagaagc gcgtgtctta ttgatctcgc cttttgataa
     2641 atcttctatc aaagatatcg agcgcgctct taacgcatca gatttaggca ttacacctgc
     2701 taacgatggc tctgtcattc gtttggttat tccagctttg accgaagaaa cacgtaaaga
     2761 attagccaaa gaagtgaaaa aagttggtga aaatgctaaa attgcaatcc gtaatatccg
     2821 ccgtgatgct atggatgatg ctaaaaagca agaaaaagca aaagaaatca ctgaagatga
     2881 gttaaaaaca cttgagaaag atatccaaaa agcaaccgat gatgctatca aagaaattga
     2941 ccgcatgacg gctgaaaaag aaaaagaatt gctctcagtg taacaggatt tctacgaaaa
     3001 atagacgact atagacacaa aaagataggt gaccatttca gaagcgtttg gttttgattt
     3061 gtccctattt ttttaaagaa agaaaaaaga tatgaatgat ttactagcaa cagtcattac
     3121 tgggcttatt aaagaagaaa acgctaatga ctattttatc caaaaagagg ggtttacctt
     3181 taccttgtct aaagcagaag gagaacgcca gattggtgat atggtcacag gctttgccta
     3241 tacagacata aagcaaaagg cacgtctcac aaccaaagag atccgttcaa cacggacaag
     3301 ttatggctgg ggggaagtga cagaggttcg tcgtgaccta ggcgtttttg tcgatacagg
     3361 aattccaaat aaagaaattg tggtttcttt ggacgtgttg cctgaaatga aagaactatg
     3421 gcctaaaaaa ggggataaac tttacattcg tttggatgtg gataaaaaag accgtatttg
     3481 gggacttcca gcagagcctg aggtctttca aaagatggct agccctgcat acaataatat
     3541 gcaaaatcaa cattggccag caattgttta ccgcctaaaa ttaacaggaa cgtttgttta
     3601 cctgcctgaa aacaacatgt taggctttat tcacccaagt gaacgctacg cagaaccgcg
     3661 cttaggtcag gttttagatg cccgtgtgat tgggtttaga gaggtcgatc gtaccctaaa
     3721 cttgtcgttg aaaccacgtt cttttgagat gttggagaat gacgctcaga tgattgtcac
     3781 ctatcttgaa gccaatggtg gtttcatgac cttaaatgac aaatcaagtc cagaagagat
     3841 taaagcaacc tttggtattt caaaaggcca gtttaaaaaa gccttgggtg gattgatgaa
     3901 agctaaacgc atcaaacaag acgccacagg aactgaatta ataggataaa aatgctttgc
     3961 cactggcaaa gttttttgtt acaatgaaga ccattaactg aagaaaaagg ggatatagag
     4021 aatggaaaga gccatttttg caggaggttg tttctggtgt atggtgcaac cttttgagga
     4081 gcaagcaggt attttgtcag tcagaagtgg ctacacaggt gggcatctcc caaatcctag
     4141 ctatgaacaa gtttgtgcca aaacaacagg acatacagaa gctgttgaaa ttatttttga
     4201 ccctaagcaa atcgcttata aagatttggt agagctctat tgggcgcaga cggatccaac
     4261 agacgccttt gggcaatttg aagatcgtgg ggacaactac cgtcctgtca tttactatac
     4321 aacggaaaga caaaaagaaa ttgcagagca atcaaaagca aacttgcaag cttcaggacg
     4381 ttttgaccaa cccattgtca ccactattga accagcagag ccattttatc tcgcagagga
     4441 ttatcaccaa ggattttata aaaagaatcc caagcggtat gcacaaagca gtgctattcg
     4501 tcatcagttt ttagaggaga attggtcatg agaaaatcat tttacagctg gctgatgaca
     4561 caacgtaacc ctaaatcaaa tgaacctgtg gcgattttag cagatttggt gtttgatgac
     4621 accacttttc caaagcatac caatgatttt gaactcatta gtcggtatct agaagatcaa
     4681 gctagttttt cctttaattt aggtcaattt gatgaaatct gggaagatta tctagcacat
     4741 taatacgtgg ataaaaaaga gtcatatcga ctcttttgaa gggttatcac gttattatta
     4801 caaaatgaca tcactttctt tcaaaaaaat gacaagctac tttttttctt aaaaatatgc
     4861 tatgatatag cttagaaaaa aataaaaagg aagcagagat gattattact aaaaagagct
     4921 tatttgtgac aagtgtcgct ttgtcgttag cacctttggc gacagcacag gcacaagagt
     4981 ggacaccacg atcggttaca gaaatcaagt ctgaactcgt cctagttgat aatgttttta
     5041 cttatactgt aaaatacggt gacactttaa gcacaattgc tgaagcaatg ggaattgatg
     5101 tgcatgtctt aggagatatt aatcatattg ctaatattga cttaattttt ccagacacga
     5161 tcctaacagc caactacaac caacacggtc aggcaacgac tttgacggtt caagcgcctg
     5221 cttctagtcc agctagcgtt agtcatgtac ctagcagtga gccattaccc caagtatctg
     5281 ccacctctca atcgactgtt cctatggcac catctgcgac accatctgat gtcccaacga
     5341 caccattcgc atctgcaaag ccagatagtt ctgtgacagc gtcatctgag ctcacatcgt
     5401 caacgaatga tgtttcgact gagttgtcta gcgaatcaca aaagcagcca gaagtaccac
     5461 aagaagcagt tccaactcct aaagcagctg aaacgactga agtcgaacct aagacagaca
     5521 tctcagaaga ttcaacttca gctaataggc ctgtacctaa cgagagtgct tcagaagaag
     5581 tttcttctgc ggccccagca caagccccag cagaaaaaga agaaacctct gcgccagcag
     5641 cacaaaaagc tgtagctgac accacaagtg ttgcaacctc aaatggcctt tcttacgctc
     5701 caaaccatgc ctacaatcca atgaatgcag ggcttcaacc acaaacagca gccttcaaag
     5761 aagaagtggc ttctgccttt ggtattacgt catttagtgg ttaccgtcca ggtgatccag
     5821 gagatcatgg taaaggtttg gccattgatt ttatggtgcc tgaaaattct gctcttggtg
     5881 atcaagttgc tcaatatgcc attgaccata tggcagagcg tggtatttca tacgttattt
     5941 ggaaacagcg attctatgcg ccatttgcaa gtatttacgg accagcctac acatggaacc
     6001 ccatgccaga tcgcggcagt attacagaaa accattatga tcatgttcat gtctccttta
     6061 atgcttaatt catgctatca aaaaaactca cgtttaggcg tgagtttttt gacaattctg
     6121 acagagtaag ccctataaga ggctgagata cgcttttaat acctgttgtt tttctcaccc
     6181 tttagacagt tttaacgtta tgtctaaaag agatggtttt tattagcgca aatatgggtt
     6241 taggggtaaa ataaaggtaa ttaacctgat atgatcatga taaaggtaga agaataccta
     6301 ttatttcgtg acaactgagc aatctgcctt acctttggag tgatgatatc actttgcttg
     6361 actattcaag gaggtcttga aatgtattat actagtggta attacgaagc ttttgcaaca
     6421 cctcgaaaac ctgaaggggt agatcagaaa tcagcttata ttgttggtac tggtttagct
     6481 ggtttagcag cagctgtttt ccttattcgc gatgggcata tggctgggga acgcattcat
     6541 ctgtttgagg aattgccttt agcaggtggt tctttagatg gtattgaaaa gcctcatctt
     6601 ggttttgtga cccgtggtgg tcgtgagatg gaaaatcatt ttgagtgtat gtgggacatg
     6661 tatcggtcta ttccctcact ggaaattcct ggtgcgtctt atttggatga attttattgg
     6721 ttggataagg atgatcctaa ctcatccaac tgtcgtttga ttcacaagag aggaaatcgt
     6781 gtggatgatg acggccagta tacgctcggt aaacagtcaa aagaattaat ccatttaatc
     6841 atgaagacag aagaatctct aggagatcaa accattgaag agttcttctc agaagatttc
     6901 tttaagagta atttttggat ctattgggca accatgtttg cttttgaaaa atggcattct
     6961 gctgtagaaa tgcggcgcta tgcgatgagg tttatccacc atattgatgg tttgccagat
     7021 ttcacctctc tcaagttcaa caaatataac caatatgatt ctatggtcaa accgattatt
     7081 gcttacctag aatcacacga cgttgacatc caatttgaca caaaagtcac tgatattcag
     7141 gtagaacaaa cagctggtaa aaaggtagca aaaaccatcc atatgacggt gtctggggag
     7201 ggtaaggcga ttgagctaac acctgatgat ttggtttttg tgaccaatgg ttctattact
     7261 gaaagtagca catacggtag tcatcacgaa gtggctaagc caaccaaagc gttaggtggt
     7321 tcttggaatt tatgggaaaa tctagctgct caatcagatg attttggtca tcctaaagtg
     7381 ttttaccagg acttgcctgc tgaaagctgg tttgtgtctg ccacagcaac cataaaacac
     7441 ccagctatcg agccttatat tgaacgtttg acccaccgtg atttgcacga tggcaaagtg
     7501 aacactggcg gcatcatcac tattacagat tccaactgga tgatgagctt tgctattcac
     7561 cgtcaacctc attttaaaga acaaaaagat aatgaaacca ctgtctggat ttacggtctt
     7621 tattccaata gtgagggcaa ttacgtccac aagaaaattg aggagtgtac aggtcaagaa
     7681 atcacagaag aatggttgta ccaccttggg gtacctgttg ataaaatcaa ggacttagcg
     7741 agtcaggact atatcaatac agttcctgtt tacatgcctt atattacgag ttactttatg
     7801 ccacgcgtca aaggagaccg tccgaaagtt atcccagatg gttcagtcaa cttggccttt
     7861 attggtaact ttgcggaatc tccatctcga gatacggtct ttacgactga gtattctatt
     7921 cgtactgcca tggaagcagt gtatagcttc ttgaatgtgg aacgaggcat tccagaagtc
     7981 tttaattcag cctatgatat tcgtgaattg ctcaaagcct tttattacct taatgataaa
     8041 aaggcaatca aggatatgga tttgccaatt cctgcactga ttgagaaaat cggacataaa
     8101 aaaatcaagg atacctttat cgaagaattg ctcaaagatg ctaatcttat gtaacatgct
     8161 ttagagtagt taaagctgat aagtttaggc ttatcagctt ttttgatgat ttatagaaaa
     8221 gtgataaagg aaaagtctga cgtgaacgtc acataagaat ttaaaatttg ttataatgta
     8281 agcaataaag atgaaagaaa gagggactct atttgcaaga gtattctatt gacattacct
     8341 taacccatcc tgatgatgtt ttagctcttt ttggtagcaa cgagcgtcat ttgaagctaa
     8401 tagaagctca tttaggagtt attgttcacg caagaaccga gcgagtccaa gtgattggtg
     8461 atgatgaaga agctgtggaa ttggctcgat tgaccatcaa agccctactg gttctagtgg
     8521 gtcgtggcat ggtggtcaac acatcagatg tggtgacagc tttatctatg gcagaatctc
     8581 atcagattga ccaatttatg gcgctttatg aagaagaaat cattaaagat aactatggta
     8641 aggcaatacg agtcaaaaca ttaggtcaaa aaacctatgt tgatagtgtc aaacgtcatg
     8701 atgtggtttt tggtgtagga cctgcaggga cagggaaaac cttcttagct gttacgcttg
     8761 cagtcactgc tctgaaaaga ggacaggtaa agcgtattat tttgacgcgt cctgcggtag
     8821 aagctggtga aagcctaggt tttttaccag gcgatttgaa agaaaaggtg gatccttacc
     8881 tcagaccggt ttacgatgcc ctttatcaca ttttaggcaa ggaacaaacc acacgcctga
     8941 tggaacgaga tgtgattgag attgctccgt tagcttatat gcggggtcgt actctggatg
     9001 acgcttttgt tatcttagat gaggctcaaa acacgactat catgcaaatg aagatgtttt
     9061 tgacccgtct tggttttaat tctaaaatga ttgtcaatgg agatacgagt cagattgacc
     9121 tccctcgaaa tgtcaagtct ggtttgattg atgctactca gaaattacaa ggcattaaac
     9181 agattgattt tgtctatttc tctgctaaag atgttgtccg acatcctgtt gtggctgata
     9241 ttatcaaggc ttatgaaacg tcctcagagg agatgaagga cttactaaaa cctaaggcta
     9301 gtgacgttga agctgactac cagccaggct tgacaaaata ccctgtgatc ggtcaagagc
     9361 actaagacct ttacgatgat gtttaatatg gcctttttaa ggaggattct atggatgatt
     9421 taaccaaagc tattatggca gatgaggcta atctctccta taccgaaaga ggtatttttc
     9481 ctttatatga tgctcctcaa acagctagga ttattattgt tggccaagct cctggaatag
     9541 tagctcaaga aaccaaactt tactggaatg atcgtagtgg catccgctta cgagactggc
     9601 taggtgtgga caatgacacc ttttaccact caggattgtt tggcattatc cctatggatt
     9661 tctattatcc tggtaaggga aaatccggtg acttaccacc tagagaaggt tttgcggcta
     9721 agtggcaccc gccattaaga gcattgatgc cagaagttga attgacgatt ttggttggtc
     9781 gctatgctca ggacttttac cttggtaaca aggcttacaa aaccttaact gaaacagtcc
     9841 gtcactttga ggattacttg ccagactatt ttccacttgt tcatccttct ccaagaaacc
     9901 aattgtggtt agctaagaac ccctggtttg agcaggattt attacctatc ttgcaaaaac
     9961 gtgtggaggc aattctaacc aaataggccc tgatttacct agcattccca tttttaaggg
    10021 gcttgtggta taatgggtaa ataacaaatg ctgattgaaa ggataaggga aattatctgt
    10081 cagataataa tgggtgccca acataccaaa tgaggtgggc agcccttccc taacaattaa
    10141 ccttatgtat atcgagatga ttgacgaaac gggacaagtt tcgcaagaga ttatggagca
    10201 aacacttgat ttgctcaatt ttgctgctca aaagacgggc aaagaagaaa aagaaatgtc
    10261 tgtgaccttt gtgaccaatg aaagaagtca tgaactcaac ttagaatacc gagatactga
    10321 tcgtccaaca gatgtgattt ctctagaata taagccagaa acgcctattt tatttagtca
    10381 agaggatttg gctgctgatc ctagtttggc agagatgatg gcagagtttg atgcttatat
    10441 tggcgaattg tttatttcta ttgacaaggc gcgtgagcag tcccaagaat atgggcactc
    10501 ttttgagcgt gaaatgggat ttttagctgt tcatggattc ttacatatta atgggtatga
    10561 tcactacacc cttgaagaag aaaaagagat gtttacctta caggaagaga ttttgactgc
    10621 ttatggcctt acacgacaat aataccacca aacggaaatg gaaaaacaga accattacct
    10681 caagcctgga gtttgccttg acaggggttt tcacagcttt taaggaagaa cgcaatctca
    10741 gaagtcacct tttgtctgcc tgtttagctt gtgttgctgg tttatttttt agcatttcag
    10801 ccatcgaatg gttattttta cttctagcca tttttttggt cattacctta gaaattgtca
    10861 attctgctat tgaaaatgta gttgatttgg ctagtgacta tcacttttct atgctagcta
    10921 aaaatgctaa ggatatggca gcaggtgctg ttttgatgat ttctggctat gctgtcttga
    10981 caggacttat tatttttatt cctaaaattt ggaatatttt cgttcattaa aaagaaagga
    11041 atgggctatc ctatgtgatg atggcatagg agaataagga aaggacaaga cggttaagct
    11101 tagtgacaag cttgatctga agccctaccg ttaaagcccg taattatcta tgtttaaatc
    11161 aggctttgta gccattcttg gtcgtccaaa tgttggcaaa tccacctttt taaatcacgt
    11221 catggggcaa aaaattgcca tcatgagtga caaggctcag accacccgta ataaaattat
    11281 gggcatttac accacagaaa cagaacaaat tgtctttatt gatacgccag gtattcataa
    11341 accaaagacc gcccttggtg attttatggt tgaatctgct tatagtactt tacgtgaagt
    11401 tgagacggtt cttttcatgg ttcctgccga tgaaaagcgt ggtaagggtg acgacatgat
    11461 tatcgagcgg ctaaaagcag ctaagattcc cgttatttta gtcatcaaca aaattgataa
    11521 ggttcatcca gaccaacttt tggaacagat tgatgacttc cgtagccaaa tggattttaa
    11581 agaagtggtc ccaatttctg ccctagaagg taataatgtg ccaaccctaa tcaagttatt
    11641 aactgataat cttgaagaag gtttccaata ttttccagaa gatcagatta ctgatcatcc
    11701 agaacgtttc cttgtgtcag aaatggttcg tgagaaggta ctgcatttga cgcaacaaga
    11761 ggttccacat tccgtagctg ttgttgtcga atccatgaag cgagatgaag agactgataa
    11821 agtgcatatc cgtgcaacca ttatggtgga gcgcgatagc caaaaaggca tcatcattgg
    11881 aaaacaagga gccatgctca aaaaaatcgg aaaaatggcg cgccgtgata ttgaactcat
    11941 gctaggtgac aaggtctact tagaaacgtg ggtcaaagtc aagaaaaact ggcgggataa
    12001 aaagcttgac cttgctgact ttggttataa tgagaaagaa tactaacata gtaaagcgag
    12061 catcatatgc cacaagacta catttcttat atccgctcca aagttgggca tgataagatt
    12121 attcttaact ttgctggtgg gattttgacc aatgatgacg gcaaggtgct gatgcagttg
    12181 cgtggtgata agaaaacctg ggctattcct ggtggcacta tggaactggg tgagagctct
    12241 ttagagactt gcaaacgtga gttcttagag gaaaccggaa ttgaggttga agctgttcgc
    12301 ttactgaatg tttacactca ttttgaggaa gtttatccca acggtgatgc tgttcagact
    12361 attgtcttta tctatgaact aacggctgtt tctgatatgg ctattgataa ctttcataat
    12421 gaggaaacgc tcaagttaca gtttttctct catgaggaga tagcagagtt agagagtgtc
    12481 tcagccaaac atcgcctgat gttagaggaa tattttagtg atagctttgc tatggggcat
    12541 taagaactct ctaggacagt tggagtattt ttggagattg gcttgttaaa aaaatttttg
    12601 aaagacgatg tttcgagagc ttatgctctc tttttttgct tccaattttt ctgattaagt
    12661 ctgaataatt ttgttaaatg ttaacaggat tttctttcca attgatacta gcagacatca
    12721 aacatttgtt ttagagttat gacgaattgc cacctagcca tgcatgactt aggcaagttg
    12781 aacctaactg tggcaataga cttggaaggg gtgacaagaa tcatttccta aaagaattga
    12841 tgaagtgcct atccattatt cgaattgtaa agttcgtcta agtaaagttg aatttgatct
    12901 cgaatggact ttaatgattt atgacttgtt gtgtcaataa caccaaaaac tcgataatga
    12961 tttttaaata tttcctcgta attaatatga tttccgatta gaataatatg agcatgagga
    13021 taac
//
LOCUS       DATURV010000045        12596 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DATURV010000045 DATURV010000000
VERSION     DATURV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12596
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.045"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            102..1094
                     /locus_tag="VCF75_001235"
     CDS             102..1094
                     /locus_tag="VCF75_001235"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEP2546797.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            1217..1756
                     /gene="rsmD"
                     /locus_tag="VCF75_001236"
     CDS             1217..1756
                     /gene="rsmD"
                     /locus_tag="VCF75_001236"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEP2546798.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            1746..2237
                     /gene="coaD"
                     /locus_tag="VCF75_001237"
     CDS             1746..2237
                     /gene="coaD"
                     /locus_tag="VCF75_001237"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEP2546799.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            2224..3261
                     /locus_tag="VCF75_001238"
     CDS             2224..3261
                     /locus_tag="VCF75_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEP2546800.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNSPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            3683..4660
                     /locus_tag="VCF75_001239"
     CDS             3683..4660
                     /locus_tag="VCF75_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2546801.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDRVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTTYVCDSQNFDFESIQ
                     LEASSNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            5084..5728
                     /locus_tag="VCF75_001240"
     CDS             5084..5728
                     /locus_tag="VCF75_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEP2546802.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            5732..6811
                     /gene="rlmN"
                     /locus_tag="VCF75_001241"
     CDS             5732..6811
                     /gene="rlmN"
                     /locus_tag="VCF75_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEP2546803.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILDDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(6893..7519)
                     /locus_tag="VCF75_001242"
     CDS             complement(6893..7519)
                     /locus_tag="VCF75_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEP2546804.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQLQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            7747..8274
                     /locus_tag="VCF75_001243"
     CDS             7747..8274
                     /locus_tag="VCF75_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEP2546805.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            8416..8619
                     /locus_tag="VCF75_001244"
     CDS             8416..8619
                     /locus_tag="VCF75_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEP2546806.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            8624..9595
                     /locus_tag="VCF75_001245"
     CDS             8624..9595
                     /locus_tag="VCF75_001245"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEP2546807.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            9605..9994
                     /locus_tag="VCF75_001246"
     CDS             9605..9994
                     /locus_tag="VCF75_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEP2546808.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRAKHILGARNFSAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            10176..12017
                     /gene="typA"
                     /locus_tag="VCF75_001247"
     CDS             10176..12017
                     /gene="typA"
                     /locus_tag="VCF75_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEP2546809.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            12139..12390
                     /locus_tag="VCF75_001248"
     CDS             12139..12390
                     /locus_tag="VCF75_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEP2546810.1"
                     /translation="MFYLIMAVLIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
BASE COUNT         4134 a         2113 c         2596 g         3753 t
ORIGIN      
        1 caaaatatta gaataaacaa aagtggacaa cgtataaaga gaactgctaa gtcgttgagc
       61 tcattagtcc caaaaagatt accaaaaaag aaggaagaac tatgaagaaa agttttattc
      121 atcaacaaga ggagatttcc tttgttaaga atacgttcac tcagtatttg attgctaagt
      181 tagatgttgt tgaagtgcaa ggacccattt taagtcgggt cggggacggt atgcaagata
      241 atttgtccgg tactgaaaat ccagtatctg tcaatgtctt gaaaattcct aatgcaacct
      301 ttgaagttgt gcattcatta gcaaaatgga aacgtcacac cttagcccgt tttgggttta
      361 atgaaggtga aggccttgtt gttaatatga aggctctccg acctgatgag gattcacttg
      421 accaaaccca ttctgtctat gttgaccaat gggactggga aaaagtcatc cctgatggca
      481 aacgtaatct cgcttacctc aaagaaaccg ttgaaaccat ttacaaggtc attcggctaa
      541 ctgaattagc tgtagaagct cgttatgata ttgaggctgt tttacctaag aaaataacct
      601 tcattcatac cgaagaattg gtagctaaat accctgattt gacgcccaaa gaacgtgaaa
      661 atgccattac taaagaattt ggagcagtct ttttaattgg tattggcggg gttttacctg
      721 atggtaaacc acacgatggc cgcgctcctg actatgatga ctggacaaca gaaactgaaa
      781 atggctatca tgggcttaat ggtgatattt tggtttggaa tgaccaactt ggttcagcct
      841 ttgaattatc ctcaatgggg atccgtgtgg atgaagaggc cctaaaacgt caggtggaaa
      901 tgactggtga ccaagaccgc ttagcctttg attggcacaa atctttgttg aatggacttt
      961 tccctctaac gattggtgga ggaatcggtc aatcacgtat ggtcatgttt ttgttgcgta
     1021 aaaaacatat tggagaagtt cagacctccg tttggcccca agaagttcgt gatagctacg
     1081 ataatatttt ataagtaatc acatcatttt gctaagtttt ccacttgtat aagagacttt
     1141 aaataaatga tcagaaatat tgaaaaaaga aatcattttt aaggtttaga gctagaaatt
     1201 tgctataata aatgttatga gagttgtatc aggtgaattt ggtgggcgtc ctttaaagac
     1261 gctcgatgga aagataacac gccctacttc agacaaagtt agaggtgcga tttttaatat
     1321 gataggacct tactttaatg gtggtcgtgt tttagatttg tttgcaggtt caggtggatt
     1381 agccattgag gctgtttctc gtgggatgtc tgctgcagtt ttggttgaga agaatcgtaa
     1441 agcgcaagct attattcaag acaatattat aatgactaaa gctgagaatc ggtttacctt
     1501 gttaaaaatg gaagctgagc gagctattga ttgcttgact ggtcgatttg acttagtctt
     1561 tttagaccct ccttatgcca aagaaacaat tgtggcaact attgaagctt tagcagctaa
     1621 aaacttattg agtgaacagg ttatggttgt ctgtgagaca gataaaacag tcttgttgcc
     1681 aaaagagatt gcaacgttag gtatttggaa agaaaaaatt tatggaatta gtaaggtaac
     1741 agtttatgtt aactaaaata ggactttata cagggtcatt tgatcctgtt acaaacggtc
     1801 atctcgatat tgttaaacgg gcaagtggcc tatttgatca aatatatgtt gggatttttg
     1861 ataatcctac caaaaaaagc tattttaaac tagaagttcg taaagcaatg cttactcaag
     1921 ctctggctga ttttacaaat gtaattgtag taacttcgca cgaacgttta gctattgatg
     1981 tagctaaaga attacgagta actcacttga ttagaggctt gcgaaatgca actgactttg
     2041 agtatgaaga aaatttggaa tattttaatc accttttagc tccaaatatt gagaccgtct
     2101 atttgatatc aagaaataaa tggcaggccc ttagctctag tcgtgtgaga gagctaattc
     2161 attttcagtc ttccttagaa ggtttggttc ctcagtcagt tattgctcaa gtggagaaaa
     2221 tgaatgaaaa gacttaaaaa aatcaaatgg tggttagtgg gtctgctagc tttaatctct
     2281 ttattgctag cgttattttt tccgctacct tattatattg aaatgcctgg aggcgcttac
     2341 gatattcgga ctgtcttaca agtcaatggc aaagaagaca aacgaaaagg agcttaccag
     2401 tttgttgcag tgggcattag tcgtgccagc ctcgcgcagc tattatatgc ttggctgaca
     2461 ccgtttactg aaattagtac agcagaagat acaacaggcg gatacagcga tgctgatttc
     2521 cttcgaatta atcaattcta catggaaaca tcacaaaatg cagctattta tcaagcttta
     2581 tccttagctg gaaaaccagt tacattagat tataaaggcg tatatgtttt agacgtaaac
     2641 aacgaatcta cttttaaagg aacgttacac ttagcagata ctgtaacagg tgtaaatggt
     2701 aaacagttta ctagttcagc agaacttatt gactatgttt ctcacctaaa actaggggat
     2761 gaagttacgg ttcagtttac gagtgataat aagcctaaaa aaggagttgg ccgtattatc
     2821 aaactgaaaa atgggaaaaa tgggattggc attgccttga ctgatcatac aagtgtcaat
     2881 tcagaagaca cagtgatctt tagtactaaa ggagtaggag gacctagtgc tggtctaatg
     2941 tttactcttg atatatatga tcaaataact aaagaagatt tacgcaaggg ccgtacaatt
     3001 gcaggtacag gaactattgg caaggatggc gaagtaggag atattggtgg tgcaggtctt
     3061 aaagtagttg cagcagctga agctggtgca gatatatttt ttgttccgaa tagtcctgtt
     3121 gataaggaaa ttaaaaaagt taatccaaat gctataagta attacgaaga agccaaacgg
     3181 gcagccaaac gactaaagac caaaatgaag attgttcctg ttacgactgt tcaagaggca
     3241 ctggtttatc ttcgcaaata aaataactat gaatgtatta tattcaatta tattcaatta
     3301 tattcacgct attaactcat aaaaaaacgg ttgaaccaga tagacagatc cttgaccagt
     3361 cttttgggtt caatcatttt ttacataaaa cataactaac ttgacagcgt aaaaaagatg
     3421 ttataaaagt ctagtggtaa aaaggcaata gagaattgag taaactttag tcgattatct
     3481 ggaaatagag catactaaaa acacctagtg aaaattgatt tttttcacta actcatccaa
     3541 gaacttgcgc agataatcat gagtccattc catgtctcta ttattcaaag ccttgcaata
     3601 gacaagttaa cgttataaca actaaattaa aaacttgaga ttgaaaaatc cccataaact
     3661 tattttacaa ggaggtaagg ctatggtaac cattaaacaa gttgctgaag aagcaggtgt
     3721 ttctagatca acagtctcca ggtatatttc tcaaaaagga tatgtatctg atgatgcaag
     3781 gcataaaatc aaagcagcga tagcaaaatt acattacact ccaaatgtct tggcgcagtc
     3841 attgaagacg aaaaaaaatc aactagtcgg tttattatta ccagacattt ccaatccttt
     3901 ttttccgagg ttagcaaggg gagcagaaga atatttaaaa gaaaaggggt atcgggttat
     3961 gctaggaaat atttcagaca gcgaagctct tgaagaagaa tacgtccatg tccttcttca
     4021 aagcaatgct gctggcatta ttactaccca tgactttaca aagcggtatc ctactcttgc
     4081 tattccagtt gtagtggtag atcgtgttga ccaagagacc cagtacggtg tcttttcaga
     4141 taatagggca gggggcttat tggcggctca gactgtctgg caagcagggg caaaagaagt
     4201 tttgttaata agaggtccgc tggataatgc tgaaaacatt aatgagcggt ttgaagctag
     4261 cttttcttac cttcaaaaac aagacgtgac tacgtatgtg tgtgatagtc agaattttga
     4321 ttttgaaagt attcagctag aagctagttc caacctcaag tgttacccaa caatagacag
     4381 tattatagca ccttctgata tccatgctat tgcttatatt catgaactgc attctcaagg
     4441 taaaaagatt cctcaagatg ttcaaataat aggttatgat gacattttaa tgagtcaatt
     4501 tatttatcct tctttatcga ctattcatca atcctcttat ttgatgggcc gttatgccgc
     4561 cgaattagtt tatactattg ctagtcaatt aactgtgaaa gcaaatcgga ttaaattgcc
     4621 tgttcactac gttgagcgag aaacaataag gaaaagataa gtaataatat tagtccagat
     4681 tttagcaggt atgatgaaga tttattggga tttaccttct actttaaaag atgttatctg
     4741 tcattacttt caccgctatc agtcaaaacg cattcttatg caattcttaa ccttaagctt
     4801 aaggtctttt tgataacata gaggaatgtt acacgacgat gacgcaataa agcctaaata
     4861 tagtaattgt cgtgatttta tagctagtga gtaatgagat agcaagtctc tcatttactt
     4921 acaaaagtca aaaaatagtg tataatggtt gagttacaga taacactgca aaaaagataa
     4981 caagtgtcca taattatcta agactataaa ggcgatatag acagatataa aaaagatgac
     5041 gaatcagtta tcactggaca attaaccgat ttagttacaa ctaatggatt tttcagaaag
     5101 gtgtcaaatg aaaaaagaaa tttcgccaga aatgtacaac tataataaat ttccaggtcc
     5161 caaatttatt cattttgaag agcaagttaa ggctgaaggc attgatttgt tactcttaga
     5221 agatgttaag aacgcttttg atacgactag ctttggtcaa cgttatacag aagttctcct
     5281 aaagtatgac tatattgttg gcgattgggg aaatgaacag cttcgtctta aagggtttta
     5341 taaggatagt gacgatatca agaaaacgaa tcgcatctca cgtttagaag attatattaa
     5401 agaattttgc aattttggtt gtgcttattt tgttttagaa aatcttcatc cacaagatat
     5461 taaatttgaa gaggagcgcc aaccaagacg aaagaaatca cctaaatcaa aatcaaatcg
     5521 tcgcaagcca aactattcaa atcagcagcc cgcaacacct aagagtaaat cgaagcgggt
     5581 atcaaaagaa aagcaacctg aaaaccaagc gtttaccagt caaaaacgtc gaagtaatac
     5641 taagcataaa gaaaagtcaa aacgtaatca gactagtcaa cttaatacca aaattagtca
     5701 ttttatcatc agaaagaaag ataaataatc catgaaacca tcgatttata gcttaacacg
     5761 cgatgaactg atcgcgtggg cagttgaacg cggccaaaaa cagtttagag ctactcaaat
     5821 ttgggattgg ctctataaaa aaagagttca atcttttgag gagatgacga atatttctaa
     5881 agattttgtt tctattttag atgatagttt ctgtgtcaat cctttaaaac agcgagttgt
     5941 acaagaatca gctgatggta cagtaaaata tttatttgaa ctgcccgatg gtatgttaat
     6001 tgaaacagtt ttaatgcgtc aacattatgg tcattctgtt tgtgtgacga ctcaagttgg
     6061 ctgtaacatt ggctgtactt tttgtgctag tggattgatc aaaaagcaac gtgatctaaa
     6121 cagtggggaa attacagctc aaatcatgtt agtccaaaaa tattttgacg atagaaagca
     6181 aggtgagcgg gttagccatg ttgtcgtaat gggcattggt gaaccttttg ataactacaa
     6241 aaatgtcatg tgctttcttc gcgtcatcaa cgatgataat ggcttagcaa ttggtgcccg
     6301 tcatattaca gtctctacct cagggttagc tcacaagatt cgggattttg caaacgaggg
     6361 agtacaggtt aatctagcag tctcgttgca tgcaccaaat aatgatttac gttcaagtat
     6421 tatgcgagta aatcgctctt ttccgctgga aaagctcttt tcagctatag aatactatat
     6481 tgaaaaaacg aatcgtcgtg tcacttttga atacattatg ctaaatgaag ttaacgatag
     6541 cattaagcaa gctcaagaat tagcagactt aactaaaact attcgtaagt tatcttatgt
     6601 taacttgatt ccgtataatc ctgtttcaga acatgatcag tatagtagaa gtcctaaaga
     6661 gcgagttcta gcgttttatg atgtgctaaa gaaaaatgga gttaactgtg tggtgcgtca
     6721 agagcacgga acagatattg atgcagcctg tggccagtta cgttctaaaa ccatgaaaaa
     6781 agatcgcgaa aaggttacag cgacaaaata aagcccaaaa aatatctttg attgacaatg
     6841 cttgatgaca aaatattcct agtcactaaa atgactaggt tttttatctg gcctaaaaat
     6901 gctctagaag cacaatccaa aataatccaa aactaagata aggaataaaa ggtatgcgct
     6961 tgatgcctaa tagtaagcaa gcagtaatac ctgctaaact agcaagttga attagccaaa
     7021 tcaacgacgt tagatccaat actaaagcta agctagcaag gtagaaaaaa tcgcctgctc
     7081 caattgaaaa aggtcttaaa tgagctaata aacctagtag cagtaatatc aatgataaat
     7141 aattgatcga ataaaacagg agtagactta caaagctaaa aagccacaag ataaaaggat
     7201 attcttgaga acgccaatca aataaactaa ataataaaga aaagagtaac aagtaacagt
     7261 aactagcact aacccaatgt accatactag cccataagat aattattaga cttatctcta
     7321 agctaaagaa gattttttgg ctagttataa aatggttttg gagctgccga aaaacgcgtt
     7381 tggcaaaagt tagttggtca caaacaatga cagtttgttt acttagctgt tcttgaccat
     7441 tatcaggaaa ataaagtagt aaacagtaaa aaagatgggt caataagatg cccgaacata
     7501 tactaaaaaa agctatcata cttatttatt cgaaaagaaa tcaaatactt gcaatctttc
     7561 cttaatcatt gtagaatata gatataaaac gataatgatt ctaattaagt atctaatcat
     7621 ccaattagga ttattccgtt attgtcgaat aggattaggt ccttaaccct ttcttatgtt
     7681 tatcacgtta gggttagttc cccctccatt cttgcccttt aagctgaaaa gtaaggagat
     7741 gttattatga caaacacact cgttgaaaat atttatgctt cagtaactca caatatttct
     7801 aaaaaagaag catcaaaaaa tgaaaaaact aaagctgttt taaaccaagc tgtcgctgac
     7861 ttatctgtgg ctgcatctat tgtgcaccaa gtacactggt atatgcgtgg tccaggtttc
     7921 ttgtaccttc acccaaaaat ggatgaatta cttgatagct tgaatgctaa tttagatgaa
     7981 atgagcgagc gtttgatcac tattggcgga gcgccttatt ctaccctagc tgagttttct
     8041 aaacactcta aattggacga agcaaaaggt acttacgata aaacagttgc tcagcattta
     8101 gcaagattgg ttgaagttta cctttacctc agcagccttt accaagttgg tttggatatt
     8161 accgatgaag aaggcgatgc tggaacaaac gatttgttta cagcagctaa gacagaggct
     8221 gaaaaaacca tttggatgct ccaagcagag cgtggacaag gcccagcact ctaatggatt
     8281 atgccttagg ataaaagaag tcaatgataa aattggcttc ttttttatgg aatcatcgat
     8341 tcattttcag aaacatagaa agcgattcca taattgtctt gaaatctgcc gtagaatttg
     8401 ctaaaatcta cttatatgaa aacactatat gatgtgcaac aattgctaaa aaactttggc
     8461 atttttgttt accttggcaa acgtctctat gatattgaaa tgatgaagat tgaacttcag
     8521 cgtctctatg atagcggctt attggataag agggattact taaatgcgga gttaattttg
     8581 cgacgtgagc atagattaga attggaaaaa gaaggataga caaatgagtc aaaaattact
     8641 ggggattgat ttaggtggta ccaccattaa atttggaatt ttaacagcag caggagaagt
     8701 ccaagaaaaa tgggcaattg agactaatat tttagaagga ggcaaacata ttgtccctga
     8761 tatcatcgct tccatcaaac atcgcttaga cttgtatggt ctaagtagtg ctgattttgt
     8821 gggaattggt atgggatcac caggagcggt tgaccgtgat actaatacag ttactggggc
     8881 attcaacctt aattggaaag aaacccaaga agtcggttcc gttgttgaaa aagaattggg
     8941 cattcctttt gccattgaca atgatgccaa tgtggctgcc cttggtgaac gttgggtagg
     9001 tgctggtgaa aataacccag atgtcgtctt catgacactt ggaacaggtg ttggtggagg
     9061 cattattgct gatggtaact tgattcatgg tgttgcagga gcaggtggtg aaatcggcca
     9121 catgattgtt gagccagaaa atggctttgc ttgtacttgt ggctcacacg gctgtttgga
     9181 aacagtagct tcagcaacag gagttgtcaa agtggcacgt ttactggcag aagcctacga
     9241 aggggattca gccatcaaag cagctattga caatggtgaa ggtgttacca gtaaagacat
     9301 tttcatggcg gctgaagcag gggattcctt tgctgattct gttgtggaaa aggttggtta
     9361 ctaccttggc cttgcttcag caaatatttc caatatcttg aatccagact cagtggttat
     9421 cggtgggggt gtgtcagcag caggagaatt tctacgctca cgtattgaaa aatactttgt
     9481 gaccttcact ttcccacaag tgaggtattc aactaaaatt aagattgcag agcttggcaa
     9541 tgatgctgga attatcggtg cagctagcct tgctcgacaa tttatcacaa aataggaggg
     9601 ggatatgtct ccaatcacac ttatcttatg gttacttctt gtcggcattg ttggttatta
     9661 tacatggaac tatttctctt ttcgaaaaat ggcaaagcag gttgataacg agacttttaa
     9721 agacgtgatg cgtcaaggtc aattgattga tttacgagag ccagcggctt ttagagctaa
     9781 gcatatttta ggagctcgaa atttttcagc gcaacaattt gatgctgcta tcaaaggtct
     9841 tcgcaaggac aaacctgttc ttatttacga aaatatgcgt ccccagtacc gtgtgcctgc
     9901 ggtcaaaaaa cttaaaaaag ctggctttga agacgtttat gtcttaaaag acggtattga
     9961 ttattgggat ggcaaagtta aacaaacaac ctagttaaga gtaacgtgga gttaagggga
    10021 ataaaggcag tcactgtctc aaaaacctta attccttttt tgctgtatcc agacttgctg
    10081 aaagtctgaa aacatttaca attgattaaa accagttttt taaaacattt tgtgttatac
    10141 ttatctagtt aaaatatatt tacttagagg aacaaatgac taacttaaga aacgatatcc
    10201 gtaacgtagc gattattgcc cacgttgacc acggaaaaac aacacttgta gatgaattat
    10261 taaaacaatc ccatactctt gatgagcgta aagagcttca agagcgtgcc atggattcca
    10321 atgaccttga aaaagaacgt gggattacaa tccttgcgaa aaatacggca gtagcctata
    10381 acgatgttcg tattaacatc atggataccc caggacacgc ggacttcggt ggtgaagttg
    10441 aacgtatcat gaaaatggtt gacggggttg ttcttgttgt ggatgcctac gaaggaacaa
    10501 tgccccaaac gcgtttcgta ttgaaaaaag cacttgagca aaaccttatc ccgatcgttg
    10561 tggtgaacaa gattgacaaa ccttcagctc gtccagcaga agttgtagat gaagtgcttg
    10621 aattattcat cgaacttggt gccgatgatg agcaattgga attcccagtt gtttacgcat
    10681 cagctattaa tggcacatca tcattatcag atgatcctgc tgaccaagag cataccatgg
    10741 cacctatctt tgatacgatt attgatcata ttccagcgcc agttgataat tcagatgagc
    10801 ctttgcaatt ccaagtgtca cttttggatt acaacgattt cgtaggtcgt atcggtatcg
    10861 gtcgtgtctt ccgtggtact gttaaagttg gtgaccaagt aactctttca aaacttgatg
    10921 gtaccactaa aaacttccgt gttacaaaac tgtttggttt cttcggtttg gaacgtcgtg
    10981 aaattcaaga agctaaagca ggtgacttga ttgctgtttc aggtatggaa gatatctttg
    11041 ttggagaaac cattacacca actgattgtg tggaagctct gccaattctt cgtattgatg
    11101 agccaacgct tcagatgact ttcttggtca ataactctcc ttttgcaggt cgtgaaggta
    11161 aatggatcac gtcacgtaag gttgaagaac gtcttttagc agaattgcaa acagacgtgt
    11221 cacttcgtgt tgacccaaca gattcgccag ataagtggac ggtttcaggg cgtggagaat
    11281 tgcacttgtc aattcttatc gaaaccatgc gccgtgaagg ctatgaactt caagtatcac
    11341 gtccagaagt tatcatcaaa gaaattgatg gtgtcaaatg tgaaccgttt gagcgtgttc
    11401 aaattgatac accagaagaa tatcagggtg caatcatcca gtctctttca gaacgtaaag
    11461 gggatatgct tgatatgcag atggttggta atggtcaaac gcgtttgatt ttcttgattc
    11521 ctgcacgtgg tttgattggt tattcaacag agtttctttc aatgacacgt ggatatggta
    11581 tcatgaatca tacttttgat cagtatctac cggttgttca aggagaaatt ggtggtcgtc
    11641 atcgtggtgc cttggtttct attgaaaatg ggaaagcaac gacatattca atcatgcgta
    11701 ttgaagaacg tggaactatc tttgtcaacc caggtacaga agtttacgaa gggatgattg
    11761 ttggtgaaaa ctcacgtgat aacgaccttg gtgttaacat tacaacagct aagcaaatga
    11821 caaatgtccg ttcagcgact aaagatcaaa ctgcagttat caagacgcct cgtatcctaa
    11881 cgcttgaaga atcattagag ttcttgaatg acgacgaata catggaagta acgccagaat
    11941 ctatccgctt gcgtaaacaa atcttgaata aagctgcgcg tgataaagct aataaaaaga
    12001 aaaaatcagc cgaatagctt ttgaagttaa actaactaaa taaaatagca aaaagatatt
    12061 taagaaaagt cattttttaa aggtcttttt gcgtttacaa gttcctatgg aatataaacc
    12121 aaaataaagg aggacggaat gttttacctt attatggctg tcttgattat atcttattat
    12181 ctatacatgg ctcctaaaag tgtccgtaac acccttggga tgattggttt agttggcttg
    12241 gttgctctgc ttattgtttt agcgggcttg agttttatca aaatcatgca aacccctcct
    12301 gaatttttta ttgggatggg aatggtggca ttgggctatt ttgctttaaa agatgtgcgt
    12361 aaaatgacca aaaaaccgag agttaaataa aagtacttaa agattgatga ttttggtttt
    12421 tatacgataa aaggactgat ttgttttaga caatatcagt ccttttttaa tagttcattt
    12481 tttctgaaag aaagctttta gccgattttc attgagtgtg caactaaaaa gagatgcttg
    12541 gtagtgacag ttaagacctc tgactagtat tttatcttat tttacttaaa aaaacg
//
LOCUS       DATURV010000046        12593 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DATURV010000046 DATURV010000000
VERSION     DATURV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12593
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.046"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(86..655)
                     /locus_tag="VCF75_001249"
     CDS             complement(86..655)
                     /locus_tag="VCF75_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2546811.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(713..1258)
                     /gene="coaC"
                     /locus_tag="VCF75_001250"
     CDS             complement(713..1258)
                     /gene="coaC"
                     /locus_tag="VCF75_001250"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEP2546812.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(1251..1943)
                     /gene="coaB"
                     /locus_tag="VCF75_001251"
     CDS             complement(1251..1943)
                     /gene="coaB"
                     /locus_tag="VCF75_001251"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEP2546813.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNKRLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            2124..2975
                     /locus_tag="VCF75_001252"
     CDS             2124..2975
                     /locus_tag="VCF75_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEP2546814.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDRNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            2972..4171
                     /locus_tag="VCF75_001253"
     CDS             2972..4171
                     /locus_tag="VCF75_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEP2546815.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVINQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNIAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            4168..5166
                     /locus_tag="VCF75_001254"
     CDS             4168..5166
                     /locus_tag="VCF75_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEP2546816.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            5208..5540
                     /locus_tag="VCF75_001255"
     CDS             5208..5540
                     /locus_tag="VCF75_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEP2546817.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            5533..6345
                     /locus_tag="VCF75_001256"
     CDS             5533..6345
                     /locus_tag="VCF75_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEP2546818.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            6338..7219
                     /locus_tag="VCF75_001257"
     CDS             6338..7219
                     /locus_tag="VCF75_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEP2546819.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            7266..8285
                     /locus_tag="VCF75_001258"
     CDS             7266..8285
                     /locus_tag="VCF75_001258"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP2546820.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KSTYQPAIEALHHLGATGVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            8413..10083
                     /locus_tag="VCF75_001259"
     CDS             8413..10083
                     /locus_tag="VCF75_001259"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP2546821.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            10168..11745
                     /gene="cls"
                     /locus_tag="VCF75_001260"
     CDS             10168..11745
                     /gene="cls"
                     /locus_tag="VCF75_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEP2546822.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGRWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLKQRP
                     WYQKLIGLLVRTIAPLL"
     gene            12084..12422
                     /locus_tag="VCF75_001261"
     CDS             12084..12422
                     /locus_tag="VCF75_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546823.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT         3912 a         2162 c         2623 g         3896 t
ORIGIN      
        1 tttccatatt ttcaagtagc taatgtaaga aagttaaaat aaccaagttt ttaaaagaga
       61 aaaactcggt tattgtgaaa gctaattacc tttttagtct tgatagtgtt ggaataagta
      121 caaaggtaat aatagctgag atgatcatct caacaatagc gtttgatgag atgatggctg
      181 ttagtagtgc tttaatattt ccatcaaaaa ctgtcgcgaa aaaaataaat atcccagata
      241 atacaaatat agtatttgtt agagaaccaa tgataccgga tactattaaa ccaaactgat
      301 taggaatcaa tttatagcaa taataagggg taataccaat caaaatacgt gggataatag
      361 caattattaa agaagcgaaa gttccatgat cgacaaaagg tgaaaagaga taatttgtag
      421 gcagtagaat gatggtattt gtaatgacac ttataatccc cattaatcct cctaaaatag
      481 cacctatcct tgggccatat aagacagaag caataataac tgggatatgg acaagcgttg
      541 gtttgattgg aataggccaa atattaaaga caagactgct cacaaaatga atgactaaca
      601 tgatggcaaa aaagatagca atacgagaaa tatctgctga tttacggtga ttcattttac
      661 tctccaaaaa atataatact taattctaaa aaggtgatca gaaaaacgac tgttaaagtt
      721 tccaaatcgt atcaatagta gctaagataa catcaatatc agccaaagca ccaggtcctt
      781 tatctccaca ggctaataag ctagattttg gaggaatttc tgtaaaccct attgtcgata
      841 agcgtttaat attttcttgg gtaatagggt tttgatacat tttggtattc atggctgggg
      901 ctattaattt tggtgtggta gctggtaggg caagagcaac acttgttaca atattatcag
      961 cgaatccata tgctagatga gcaatagtat tagcagaggc aggtgcaaca ataaataaat
     1021 ctgtgcgttt tgctaattcg atatggttga taacttttgg gtcatgctca tccataacat
     1081 ctaaatgaat agcatttttt gataacactt gcagggttaa tggtgtaata aattgtgtag
     1141 cagcctgagt cattatgatg tgaacatcat agccaatttt agttaattga ctcgtaagat
     1201 ctgctgcttt ataggcagat atactaccag aaacagctaa ggtaatatgt ttagtcatgt
     1261 ttggtcatcc tttcatataa taagtcagca attgcctctt tggtatctgc agatgcaact
     1321 tcgttattgg atatcaaaag agccttgtgc atacctgttt ggatatcaac taaatcattg
     1381 gcgagaatat aatcagcatg gtttttagct agacttgctc tggctacttt aattaactct
     1441 tcttgaggca cattaactag aagtttaaag ccgactagtt taatattagg attccactgt
     1501 ttaacataag agataacttt gggtgttttt ttcaaaaaaa gaacctgata atcagatgca
     1561 gatgagattt tcggctcact gttatgttca catagaaaac aattaaggtt atcagcttga
     1621 gagacttgtt ccaaatcagt catgtagact ggagtatagt cagaaacagc catactatga
     1681 attagaatgt catggtgagg aacctgatct ttgagtgcag ccattaagtc gttaactgtt
     1741 tccacttcaa taatccttag gcgcttattt ggcaggggtt tagtggctgt tttagtagtg
     1801 actagggtaa cgtcatgatg atattgtaaa aatcgctctg tgattaattt tccaagttgc
     1861 ccagttgagt gattagtgat acctcgtact gcatctatag gttcagttgt tccacctgat
     1921 gttattatta atttcatagt catattttat caaaaaagga gctgatagta aacggaagat
     1981 aatgacgcca gacattgtct tgcgtttgta ttaatatgga atagtaatag atttctagta
     2041 attaaaacga agttctggaa agcaagacac tatagtagct aaaatctaaa ttttaaggta
     2101 aaatagagca agcggtttca agtgtgaaag gaagaattgt gataactatt agagatttag
     2161 cgtcactgcc gatttttgtt tacggtgata gaaataaaaa agttccagga gctttgagtc
     2221 cttttgtgtg gacagaggtg tttttaaaaa ctatcaatca agaacctaat caactgatct
     2281 tacatatttg gccgatgaca agaacagtta tattagggat gttagataga cagcttccct
     2341 actttgagct ggctaaaaca gagattggca ataatggtta tgttcctgtt actcgtaata
     2401 ttggcggttt ggctgttgtc gctgatgatg ggattttgaa tttttcgctt gttattccag
     2461 accatttttc ggaatctatc agtattagta atgcttattt aataatggtt gatgtcatta
     2521 gagagagttt ttcggattat tatcagcgta ttgaatatca cgaaattaaa aattcttatt
     2581 gccctggtaa ttttgatctt agtattgcag gccgcaagtt tgcaggtatc gcccagcgtc
     2641 gtatcaaaaa gggaattgtt gtgtctattt acttgagcgt ttgtggggat caagcagcgc
     2701 gtggtcaact catcaaggat ttttatgagg ccggaacaca aggagaagtt accaaggtta
     2761 attaccctca aattgatcca gaatgcatgg caactctttc agaactcctt gagacgcctt
     2821 ttacagtggc agaagtcctt gaacgtcttc gattgacctt gcgacagctt ggcttttccc
     2881 tcacggaaaa aagtccagat caagccttac tgactaattt tgatgctgtc tatgaacgga
     2941 tgcagctaga agtcgtcaga aaggaaggca aatgaaagaa agatttagtc ctttatttga
     3001 gcctttgact ttgccaaatg ggagtcaatt ggataatcgc tttgttttgt ctcctatggt
     3061 gaccaactca tccacaaaag acggttatgt cacccaagat gatgtatcct atgctttgag
     3121 acgagcagct tctgcccctc tacaaattac aggagcggcc tatgtggatc cttatggtca
     3181 attgtttgaa tatggtttta gtgttacaaa agatgccgat atttctggct tgaaagagct
     3241 agctcaagcg atgaaggcaa agggagctaa agctgtctta caactgaccc acgctggccg
     3301 gtttgctagc catgccttga ctaaatatgg ctttgtctat gggcctagtt acatgcagtt
     3361 gagatctccc caacctcatg aagtcaaacc tttaacaggt cagcaaatag aggaattgat
     3421 tgcagcttac gctcaggcca ctcggcgggc aattcaagca ggctttgatg gcgtggaagt
     3481 atcttcggcg cagcgcttat tgattcaaac gttcttttcg actttttcga acaagaggac
     3541 agatagttat ggttgtcaaa cactttttaa tcgctctaag ttaactttag ctgttttgca
     3601 agctgttcaa caggtgatta atcaagaagc tcctgatggg tttatttttg gatttcgtgc
     3661 tacaccagaa gaaactaggg gaaatgatat tggttatagt attgacgagt ttttgcagtt
     3721 gatggattgg gtacttaata ttgcaaaact tgattattta gcgatagcaa gttggggccg
     3781 tcatgtattt cgaaatactg ttcgttctcc tggcccttat tatggaaggc gagttaatca
     3841 ggtagttaga gattatttaa gaaataagct acctgttatg gctacagggg gaatgaacac
     3901 acctgacaaa gctattgaag cactagctca tgctgatttt attggagtat ctacaccttt
     3961 tgtcgtggat ccagaatttg ctcataaaat caaagaaggg tgtgaagaga gtattcattt
     4021 aaggattcga cctgctgatt tgaaaagttt agcgattcct caggcttctt tcaaagatat
     4081 tgtacctttg atggactacg gggaatcatt gcctaaagaa tcaagaactt tatttcgtag
     4141 tcttactcac aattataagg agattaaatg aaagtcagta ttcttgatta tggggtgatt
     4201 gataaagaaa aaacacccca agaagcttta ttagaaacgc gctgcttagc acaagtagcc
     4261 gacaaacttg gttttcatcg tttttgggta gcagagcatc ataatattta tgcctttgca
     4321 ataagcagtc cagagctgtt gatgatgcat ttagcagatc ataccaaaca gattcggata
     4381 ggttcaggag gtattatgcc tctacattat agtagcttta agattgcaga atggataatg
     4441 actttagagg ccttgcaccc taatcggatt gatttaggta ttggcaactc tttaggaact
     4501 acgttagttc aaagagctct atcaagtatc cattgcaaag acagctatag tcaggttgtc
     4561 acagaattat accagtatct taatcctgat catttgtcac cactaccgat ttttgttaac
     4621 cctaggggta atacttaccc tcagatttgg acgctaagca atagtttgga gacagcagaa
     4681 ctggctggtc aattaggttt aggctatacc tttggtattt tcccctatat accaaaagat
     4741 cctataacag aagctaaaag agtatcggca cattatagaa aggcttttag gccttcaaag
     4801 ctattaaaaa tccccaagtt aatattagca gtttttattg tcctttctga taccgacgag
     4861 gaagcagaag ctttggctaa accgttggac atttggatgc ttggtcagca agattttaat
     4921 gaatttaaga cctaccctga tgttgaggag gctagaaact atcacttaac agagaaacaa
     4981 agagaagcca ttgcagcaaa tcgtagtcgc atggttattg gatcacctca cacagtcaag
     5041 aaacaattgg ataggctgat agaagcttgt caagctgacg aattattagc catcccctta
     5101 gttcctgaat ttgccaatag acaacgaacc ttagagctgc tagcagactt atacgtgacc
     5161 atttaacctt accaaaaaca attccctaaa ctgaaaggaa agaaacaatg aaaaaaattg
     5221 caaattactt attaattgag aaaacagatg accgctatac cattagcatg acaccagaat
     5281 tacaagacga tattggtact attggttacg ctgaatttac ggataacgac caccttgccg
     5341 ttgatgacat tatcttaaat ctagaggctt ctaagaccgt catgtctgtc ttgtcaccat
     5401 tggctggggc agttgttgag cgcaatgagg cagccacact aacaccaacc cttttgaatt
     5461 cagaaaaagc tgaggaaaat tggattgttg tgctaacaga tgttgatcaa gcagcttttg
     5521 atgccctaga agatgcctag ttcatttgat ttattagggg aaatgattgg tctgttgcaa
     5581 acagagcagc ttactagttc ttgggcctgt cccttgccta atgctttaac aaagcgacaa
     5641 gacttgtggc gggcgctcat taaccagcgc cctgctttgc ccttatcaaa agattacttg
     5701 aatctggaag atgcttatct agatgattgg cgtgcttctt ttgttcctgt atcagtcaag
     5761 gattgccaaa aaactaatta taccagtctt tttctttacc atggggatat taggtacctt
     5821 gctgtggatg ctattgtcaa cgctgctaat agtgagttat tgggatgttt tattcctaac
     5881 catggctgta ttgataatgc gattcatacg tttgccggaa gtcgtttacg cttagcttgt
     5941 caggcaatca tgactgaaca aggccgtaag gaagccattg gacaagcaaa gcttaccagt
     6001 gcttatcatc taccagcctc ttatattatt cataccgtag ggccaagaat taccaaagga
     6061 caccacgttt ctccgattcg agctgatttg ttagcacgtt gttatcgttc tagtcttgat
     6121 ttggcggtta aagcaggatt gactagccta gctttttgct ctatttcaac aggagaattt
     6181 ggctttccta aaaaagaagc cgctcagatt gctattaaga cagttttaaa atggcaagca
     6241 gaacatccag aatctaagac cttaactatc atttttaata cctttacatc agaagacaag
     6301 gccttatacg acacttattt gcaaaaggag aataactgtg agtaattgga caacctatcc
     6361 tcagaaaaac ctcacccagg cagaacaact ggcccaatta atcaaagaag cagatgcact
     6421 tgtagttggt attggagctg gcatgtctgc agctgatggt tttacttata tcgggccgcg
     6481 ttttgagacg gcattcccag attttattgc taagtatcag tttttggata tgctgcaagc
     6541 tagtcttttt gattttgaag attggcaaga atattgggct tttcaaagtc gctttgtcgc
     6601 tttgaattat cttgatcagc ccgtaggcca gtcttatctt gatttaaaag agattttgga
     6661 aaccaaagac tatcatatta ttactactaa tgctgataat gctttttggg tggctggtta
     6721 tgatcctcat aacatttttc acattcaagg ggaatatggt ttgtggcaat gtagccagca
     6781 ctgccatcag caaacttata aggacgatac ggtcatccgt caaatgatcg cagagcaaaa
     6841 aaacatgaaa gtcccaggtc aactgattcc tcattgccct gaatgtgagg cgccttttga
     6901 aatcaataag cgtaatgaag aaaaaggaat ggttgaagat gctgactttc atgcccaaaa
     6961 agcacgttat gaggcttttt tatcagaaca taaagagggt aaagtgcttt atctagaaat
     7021 tggtgtggga catacgacgc ctcagtttat caaacacccc ttttggaaac gtgtctcaga
     7081 aaaccctaat gccttattcg ttaccttgaa tcacaaacat taccgcatcc ctctgtcaat
     7141 aaggcgacaa agcctagaac tgacagaaca cattgcacaa ttgattagcg ccacaaaaac
     7201 catttaccaa aaatcgtaac caaagccaat agacggttaa ttaagagata tagaaggaga
     7261 ctaacatgta ccttattgaa ccgattcgta acggtaaacg tatcaccgat ggagctgttg
     7321 ctctagccat gcaagtttat gttcaagaaa atctattttt agatgatgat attttatttc
     7381 cctactattg tgatcccaaa gttgaaattg gaaaattcca gaatgcggtt gtagaaacta
     7441 atcaagagta tttaaaagag catcatatcc ctgttgttcg ccgagatact ggcggtggtg
     7501 ccgtttatgt agattctggt gctgttaata tttgctactt gattaatgat aatggtattt
     7561 ttggtgattt taaaagtacc tatcagcccg ctattgaagc ccttcatcat ttgggagcaa
     7621 caggggttga aatgtctgga cggaatgact tggttattga tgggaaaaag gtatctggcg
     7681 ctgcgatgac tattgctaat ggccgtgttt atgggggata ttcccttttg ctagatgtgg
     7741 actttgaagc catggaaaaa gcattaaaac ctaatcgcaa aaaaattgaa tcaaaaggca
     7801 tcaggtcagt tcgtagtcgc gttggaaaca tcagagaaca tttagcacca caataccaag
     7861 gtattacgat tgaagagttc aaagatttaa tggtttgcca attgttacag attgagacga
     7921 ttagccaagc taagcgttat gatttaacgg aaaaagactg gcaacaaatt gatgctttaa
     7981 cagagcgtaa atatcacaat tgggaatgga attatggcaa cgcgcctcaa taccgttacc
     8041 atagagatgg ccgttttacc ggtggaacag tagatattca tttagatatt aaaaaaggct
     8101 atattgcagc ttgtcgcatt tacggagatt tttttggcaa ggcagacatt gcagaacttg
     8161 aaggtcactt gataggaaca cgaatggaaa aagaggatgt cttagctacc ctcaatgcaa
     8221 ttgatttagc cccttattta ggtgcgatta cagcagaaga attaggagat ttgatatttt
     8281 cttgataaaa aagatacctc agaccggtaa agtgcgatta aaaacgaaca ataattgttt
     8341 taattcaaat aaatgtacgt atgatatgaa ttgtgatata atgacgtcat tacgtaaaat
     8401 tgaggagtca tcatgaaatc agatatcgaa attgcccaaa gtgtcgccct acaaccaatc
     8461 actgacattg ttaaaaaagt tggaatagac ggcgatgaca ttgagcttta tggcaaatat
     8521 aaggctaaat tgtcttttga gaaaatgaaa gctgtcgaag ctaatgagcc tggaaaatta
     8581 atcttggtaa ctgctattaa ccctactcca gcgggagaag gaaaatcaac catgtctatt
     8641 ggactggcag atgccttaaa tcaaatggga aaaaaaacca tgcttgcctt acgtgaaccc
     8701 tcccttggtc ctgttatggg gattaaaggc ggggcagcag gtggtggtta tgcccaagta
     8761 ttgcctatgg aagacattaa tcttcacttt actggtgaca tgcatgccat tacaactgct
     8821 aataatgcat tatctgcttt gattgacaat catttgcagc aaggaaacga tttaggaatt
     8881 gatcctcgcc gcattatttg gaagcgcgtg ttggacctta atgatcgcgc tcttcgtcag
     8941 gtaattgttg gcttaggtag tccagtgaat ggtgtgccac gcgaagatgg atttgatatc
     9001 acagtagctt ctgaaatcat ggcaatttta tgtttggcaa ctgacttaaa agatctaaag
     9061 aaacgtcttg ctgatatcgt cgtggcttat acctatgatc gtaagcctgt ctatgtgcgt
     9121 gacttgaagg ttgaaggagc attgacatta attctaaaag atgctatcaa gccaaacttg
     9181 gtgcagacta tttatggaac accagctctt attcatgggg ggccatttgc taatattgct
     9241 catggttgta attctgtcct tgctacctca acagctttgc gattagcaga ttacactgtt
     9301 accgaagcag gattcggggc cgacctagga gctgaaaaat tcctaaatat caaagtacct
     9361 aatcttccta aagcaccaga tgctatcgtc attgttgcaa ccttgcgcgc ccttaaaatg
     9421 catggaggtg tcgccaaatc agatttagcc gctgaaaatt gtgaggctgt tcgcttagga
     9481 tttgccaact taaaacgtca cgtggaaaac atgcgccaat tcaaagttcc agttgtggtg
     9541 gctatcaatg aatttgtagc agataccgag gctgaaattg ctaccttaaa agcgctttgt
     9601 gaagagatta aggttccagt agaattagcc agtgtttggg caaatggagc tgagggaggc
     9661 ttagcattag ctaaaacagt ggttcgtgtg attgatcaag aagctgctga ctacaaacgt
     9721 ctctattcag atgaggatac tttggaagaa aaagtgatca atatcgttac ccagatttat
     9781 ggtggcaaag ctgttcaatt tggccccaag gccaaaacac aattgaagca gttcgcagaa
     9841 ttcggttggg ataaattgcc tgtttgtatg gctaagaccc aatacagttt ttcagataac
     9901 ccatccttgc ttggagcgcc aaccgatttt gatattacca ttcgtgaatt tgttcctaaa
     9961 acaggggcag gttttattgt tggtttaaca ggtgatgtga tgaccatgcc tggtttgcct
    10021 aaagttccag ctgccatggc aatggacgta gcagaaaatg gcacagccct cggtctcttt
    10081 taggaaaaat tgctcgaata attcgagcaa gcgctccttt cttcccgctt tactgtgata
    10141 taataaggta aatgagattt tggagggatg attatcaaaa agaaagcaaa agtaaaatat
    10201 ttgctccata aagggaaaca tggattttta agaggaattt ttagtagaac aacaatcatt
    10261 gtattattaa ttattttgca attagttttc ttatttcaat cttatgcctg gatggagcag
    10321 taccgtgttt ggataacgat tcttgaaagt gtctttgcta ttactattgt tttatattta
    10381 gttaatagtg atatggatgc tatttcaaga atgacgtggt taattttgat tatgattgct
    10441 ccattactag ggtcactgtt tttaatttat acaaaattgg attggggtta cagagggcta
    10501 aaacaaagaa taaatcatct tgtagatctg tctgcacctt accttagcga tgatgacgct
    10561 atattagagg tattaaagga tagcacatca accacatatc atttagtaca gtacttagaa
    10621 agaagtcgtg gcaactttcc gatttataat aatacaaggg taacttactt cccaacaggt
    10681 gaaacttttt ttgatagttt aaaagaacag ttattcttag ctaaaaagta catttttctt
    10741 gaatttttta ttattgcaga aggccaaatg tggggagaaa tccttagtat tctagaaaaa
    10801 aaagtcagtg aaggtgtaga agttagggtg ttgtttgatg gcatgaacga actatcaacg
    10861 ctatcatcag attacgccaa gagattagaa caaataggga ttaaagctaa atcattttta
    10921 ccgatttcac cctttatctc tacctattac aactatcgag atcaccgaaa aattgtcgtt
    10981 attgatgggg aggtatcatt cactggaggt attaatctag cagatgagta cattaatgaa
    11041 gtagagcgtt ttggccgctg gaaagatgct ggtttaatgc ttgagggtga agcaacagac
    11101 agctttttaa ttttgttttt acaaatgtgg tctatcacag aaaaagaact gattattgat
    11161 ccttatcttt cagatcattc tttaaaactt ccttcagatg gctatgttat tccctacggt
    11221 gattccccgc ttgatactga taaaataggt aaaaatgttt atatagacat tttaaatcat
    11281 gctaaagagt acgtttatat catgacacct taccttattt tagatagcga gatggaacac
    11341 gctttacgat ttgcatcaga acgtggagta gatatccgaa ttattatgcc aggagtgcca
    11401 gataaggggg taccatatgc cttagctaaa acctattata aagctttgat gacttctgga
    11461 gtaaaaattt atgagtatca accagggttc gttcattcaa aggtatttat ctctgataat
    11521 acaaaagcag ttgtcggaac aattaactta gactatcgta gcctttatca tcattttgaa
    11581 tgcgcgactt atttatatcg tgtgtcagtt attgctgata ttgtgaatga ctttaatgaa
    11641 gctcaaaagc aatcactatt gatgacatca gatcatttga agcaacgtcc ttggtatcaa
    11701 aaattgatag gattattagt acgaacaatt gcccctctct tgtaattttc gtaaaatatg
    11761 ttataatatc tgtatgcgat gagtcgattg tgactgcaaa gtcacttttg cgcaggggag
    11821 gtcattaagg cgggaagccg actttgataa tctgtgtgaa ccattttatc acaatcttat
    11881 ttattaagaa tgccccttgg attgagatat tctcaatcct tttttaattt tattatctta
    11941 gttaaaattc agaatgtatc agtgattact gtgtttcaaa ataagaaagt agtttttcgg
    12001 gtatgagtgc tatgacttga caaatgctaa taacatttgc ataatgtaag tagagatgaa
    12061 aacttatgaa aagagatgat cttatgcaaa ttattaagcg tgatggccac gttgctgatt
    12121 ttgatccaga taaaatttat caagcaatta ttaaagcagc aaggacggtc tatgttattg
    12181 atgacacgtg gcgacaaaac ttaacacatg tcactaaaaa agtagttatt gatttagaag
    12241 aagcacatgt agaacgtcct actatcaata tggtccaatc ccttgtggag aatcgtttga
    12301 tggatgctgg atatattacc attgcagaac attacatttc ttatcgtctt cagcgagatt
    12361 tagagcgcaa tggctatggt gataagattg tggttcactt gcgttttgaa caaataaaat
    12421 aaaaacgctt ctaatggcat tttagtgtct ttgaggggat aaggtttaaa tacctaacag
    12481 ataaaaatct gttaggtatt tttttgctac tattaggtgt attctatctc acatagaact
    12541 taagcattca agctttaatt taactttctg tttatttttt gctacatttt ggg
//
LOCUS       DATURV010000047        11008 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DATURV010000047 DATURV010000000
VERSION     DATURV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.047"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            108..815
                     /locus_tag="VCF75_001262"
     CDS             108..815
                     /locus_tag="VCF75_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEP2546824.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLRVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            878..1933
                     /gene="prsA"
                     /locus_tag="VCF75_001263"
     CDS             878..1933
                     /gene="prsA"
                     /locus_tag="VCF75_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP2546825.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            2320..4938
                     /gene="alaS"
                     /locus_tag="VCF75_001264"
     CDS             2320..4938
                     /gene="alaS"
                     /locus_tag="VCF75_001264"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEP2546826.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRN
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            5278..5493
                     /locus_tag="VCF75_001265"
     CDS             5278..5493
                     /locus_tag="VCF75_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2546827.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            5490..6251
                     /locus_tag="VCF75_001266"
     CDS             5490..6251
                     /locus_tag="VCF75_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEP2546828.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVFSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            6270..6965
                     /locus_tag="VCF75_001267"
     CDS             6270..6965
                     /locus_tag="VCF75_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP2546829.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIVSTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(7057..8214)
                     /locus_tag="VCF75_001268"
                     /pseudo
     CDS             complement(7057..8214)
                     /locus_tag="VCF75_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558257.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8292..9602)
                     /locus_tag="VCF75_001269"
     CDS             complement(8292..9602)
                     /locus_tag="VCF75_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEP2546830.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTIFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            9826..10698
                     /locus_tag="VCF75_001270"
     CDS             9826..10698
                     /locus_tag="VCF75_001270"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEP2546831.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEAESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         3445 a         1928 c         2350 g         3285 t
ORIGIN      
        1 tacattttag gattaatggt agcattaagg ttaaagaaat gatgtcctat aaaaaattta
       61 aaagtaaacc ctagcaaagc ctagggtttt tttatataat taggtttatg gttaaatcat
      121 acagtaaaac agcaaatcat aatatgcgtc gtcctgttgt caaagaagag cttgttcatt
      181 atatgcgaac acggcagaag caaaccacag gttttttagc agagttagag caattcgcaa
      241 gacaagaaaa cattcctatt attcagcctg aggtcgtggc ttatttcagg tttttattgc
      301 aaagcttaca gccaaaacac attttggaga ttggcacagc cattggtttt tctgcgcttt
      361 taatggctga aaatgcacca gatgcgacga ttgtgactat tgatcgtaat cgagagatga
      421 tcgattttgc taaagcaaat tttgctaagt acgacagtcg ccaacagatc cgcctgttag
      481 aaggagatgc agcagatata ttatctacac ttgaaggaaa ttttgacttt gtgtttatgg
      541 attccgccaa atccaaatat attgtctttc taccagagat tttaagactg ttaagagtcg
      601 gtggtgtagt cattttggat gatgtctttc aaggaggtga cattacaaaa ccgatcgaag
      661 acattcgacg aggtcagcgt actatttatc gtggcttaca gtctttgttt gatgccacct
      721 taactcatcc aaacctaaca actagcctgg tccctcttag tgatggatta ttaatgattc
      781 gtaaaaatca agcagatatt gtattaccag attaagtaac attgataaat ttatgttata
      841 ataaattagt taaagataaa aaggagctca gaagtaaatg aaaaactcaa ataaactcat
      901 tgctagtgtt gtgacattgg cctcagtgat ggctttagca gcttgtcaat caactaatga
      961 caatactaag gttatttcga tgaaaggtga tacaattagc gttagtgatt tttacaatga
     1021 aacaaaaaac acagaagtat cgcaaaaagc gatgctaaat ctggtaatta gtcgtgtttt
     1081 tgaagctcaa tatggtgata aggtttcaaa aaaagaagtt gaaaaggcgt atcataaaac
     1141 agctgaacag tatggcgctt cattctctgc tgctttggca caatcaagct tgacacctga
     1201 gacttttaag cgtcagatcc gctcttcaaa attagtagaa tatgcggtta aagaagcagc
     1261 taaaaaagaa ttgacaacac aagaatataa gaaagcatat gaatcttata ctccaacaat
     1321 ggcagtcgaa atgattactt tagataatga agagacagct aaatcagtct tagaggaact
     1381 aaaagccgaa ggcgcagact ttacagctat tgctaaagaa aaaacaacaa cacctgagaa
     1441 aaaagtgacc tataaatttg attcaggcgc gacaaatgta ccgactgatg tcgtaaaagc
     1501 ggcttcaagt ttgaatgagg gtggaatatc agacgttatc tcggttttag atccaacttc
     1561 ttatcaaaag aagttttaca ttgttaaggt gactaaaaaa gcagaaaaaa aatcagattg
     1621 gcaagaatat aagaaacgtt tgaaagctat cattatagct gaaaaatcaa aagatatgaa
     1681 tttccaaaac aaggttattg caaatgcatt ggataaagct aatgtaaaaa ttaaagacaa
     1741 agcttttgct aatattttgg cgcaatatgc aaatcttggt caaaaaacta aagctgcaag
     1801 tgaaagttca acaaccagcg aatcatcaaa agctgcagaa gagaacccat cagaatcaga
     1861 gcaaacacag acatcatcag ctgaagaacc aactgagact gaggctcaga cgcaagagcc
     1921 agctgcacaa taagtaggat tttattgact aatcagttaa aaaaacttat aataatttag
     1981 ttaagaattg tttatcaagg attagataca gaaaagtgac ggttgctgcg agtcattgat
     2041 aattagataa acatgctact taactttaca acaattgata tcgtaaatta agaatgacaa
     2101 gttcattgaa tagaggtggt accgcggttt tcgccctctg tgagatggac ttgttttgta
     2161 ttgaggacta tttgaaatca acgaaaggaa tagagtagac taggctgcca ggcttatagc
     2221 tgatctccta gcaagaaaga cgagctgtgt caactggtgt gattacatta ctagcttcag
     2281 cataacactg tttttcttac tctttgtatc taaattttta tgaaagaatt atcgtctgca
     2341 caaatccgcc aaatgtggtt ggatttctgg aaatccaaag gacattgcgt tgagccttca
     2401 gctaacttgg ttcctgtgaa cgacccaacg cttctttgga tcaactcagg tgttgcaacc
     2461 ttgaaaaaat attttgatgg ttcagtgatt ccagaaaatc cacgtattac caatgcacaa
     2521 aaatcaattc gtactaatga tattgaaaat gttggtaaaa cagcacgtca ccatactatg
     2581 tttgaaatgc ttggtaactt ctcaattgga gactatttcc gtaatgaagc tattgagtgg
     2641 ggatttgaac tcttgacaag tccagactgg tttgatttcc ctaaagacaa gctctacatg
     2701 acttattacc cagatgacaa ggattcgtat aaccgttgga ttgcttgtgg cgttgaacca
     2761 agtcacttgg tgccgatcga ggataacttc tgggaaatcg gtgctggtcc ttcaggtcca
     2821 gatacggaga ttttcttcga tcgtggtgaa gatttcgatc cagaaaatat cggacttcgc
     2881 ctcttggctg aagatatcga aaacgatcgt tacatcgaaa tctggaacat cgttctctca
     2941 caattcaatg ctgacccagc cgtaccacgt tcagaataca aagaattacc aaacaaaaac
     3001 attgatacag gtgctggtct tgaacgtctt gcagctgtta tgcaaggggc aaaaacaaac
     3061 tttgaaactg accttttcat gccaatcatc cgtgaagtag agaagttgtc aggtaaaact
     3121 tacgatccag atggcgacaa catgagtttc aaggttatcg ctgaccacat ccgtgcgctt
     3181 tcatttgcta tcggtgatgg tgcgcttcct ggaaatgaag gtcgtggtta cgttcttcgt
     3241 cgtcttctcc gtcgtgcggt tatgcatggc cgccgtcttg gcatcaacga aactttcctt
     3301 tacaaattgg ttccgactgt tggacaaatc atggaaagct actacccaga agtgcttgaa
     3361 aaacgtgatt ttatcgagaa aatcgttaaa cgtgaggaag aaacatttgc tcgtactatc
     3421 gatgcaggta gcggtcactt agattcattg cttgcgcagc ttaaggctga aggtaaggat
     3481 actcttgaag gtaaagatat cttcaaactt tatgatactt atggattccc ggttgaattg
     3541 acagaggaat tggcagaaga tgcaggctac aagattgacc acgaaggctt taagtcagcc
     3601 atgaaagaac aacaagaccg tgcgcgtgca gctgttgtta agggtggttc aatggggatg
     3661 caaaatgaaa ccctagctgg tattgttgaa gaatcacgat tcgaatacga cacatatagt
     3721 cttgaatcaa gtctttcagt catcatcgct gataatgaac gtaccgaagc tgtttcagaa
     3781 ggtcaagctc ttcttgtctt tgctcaaaca ccattctatg ctgaaatggg tggacaggtt
     3841 gctgacacag gtagaatcaa aaatgataag ggtgacacag ttgctgaggt tgttgatgtt
     3901 caaaaagcac ctaatggtca acctctacac actgtaaacg ttttggcatc actttcagtt
     3961 ggaacaaact acacacttga aatcaacaaa gagcggcgtt tggctgttga gaaaaaccac
     4021 acagctactc acttgctcca tgcagctctt cacaatgtta ttggtgaaca cgcaactcag
     4081 gctggttcat tgaacgaaga agaattcttg cgctttgatt ttactcactt tgaagcagta
     4141 agcaatgagg aacttcgtca cattgaacaa gaagttaatg agcaaatttg gaacgctctt
     4201 acaatcacaa cgactgaaac tgacgttgaa accgcaaaag agatgggagc aatggcactt
     4261 tttggtgaga aatatggtaa agtggttcgt gtggttcaaa ttggtaatta ttctgttgaa
     4321 ctttgtggtg gaactcactt aaataattct tcagaaatcg gtctcttcaa gattgtcaaa
     4381 gaagaaggta ttggttcagg cactcgtcgt attattgcag ttactggtag acaagctttt
     4441 gaagcttatc gtaaccaaga ggatgcccta aaagagatcg ctgctactgt aaaagctccg
     4501 caattgaaag atgcagcagc taaagtacaa gctcttagcg actcacttcg tgatcttcaa
     4561 aaagaaaatg cagaacttaa agaaaaagca gcagctgcag cagctggtga tgtctttaaa
     4621 gatgttcaag aagctaaggg cgtgcgcttc attgctagtc aagttgatgt tgcagatgca
     4681 ggggcacttc gtacatttgc tgataactgg aaacaaaaag actactctga tgtgcttgtt
     4741 ctcgtagcag ctattggtga gaaggttaat gtccttgttg caagcaaaac caaagatgtc
     4801 cacgctggta acatgatcaa agaattggca ccaattgtag caggtcgtgg tggaggtaaa
     4861 ccagacatgg ctatggcagg tggtagcgat gcaagtaaaa ttgcagagct gctagcagca
     4921 gttgctgaaa cagtgtaaac ccataaaaaa ccaagttatt tagaacttgg ttttttatat
     4981 ttgaaatgac tattatcgtt gtcaaaaaga aaaagatatg ttacaatgat agtaacaaat
     5041 agacatgagg aggtgttaga gaaaatgaca catggtatca aacttaagag gcagatttgc
     5101 ttatgggcaa gtatcctgac tcttttcatt ttaagttatc atcttatcag ggtaatctgg
     5161 tttggagtac ccttgagtta tctggttttg ggctttacag gactagcctt gctgttgaat
     5221 actttttctt attttagaaa gtaagttata agttaaagaa gaatgaagga gatggcaatg
     5281 gaggttattc ttaaaaatcg gctcaaagaa ttacgagcca gagatggaat taatcaaacg
     5341 gaaatggcta aactggcagg agtatcgagg cagaccatca gtctcattga gcgtaacgag
     5401 tacaccccat cagttataat tgccatgaaa attgcaaagg tatttcagga accagttgaa
     5461 gaggtgtttc gtctagtgga ggtcgaggaa tgaaaaagaa gaagaatagt tttttacgct
     5521 tgttaaaaat gttattgcta tcaagtttgg cgggagggat tataggtggc atggttggag
     5581 cctttttagg ttaccatgga gagagattag atcagctcac gttcctaaaa gatgatgtga
     5641 ttaacctgat tatcttatta aatcgtctag tagttgtcac aggcctcaca ctttcttttg
     5701 tttttttaac gcaactgaaa aaagagacag ctgtttataa cacaattgaa gaggatgatt
     5761 attctgaaaa tggttaccgt cagctgaata aaaagcatgc ttataccatg ttattgattg
     5821 ccgtagcttc tattttatcc atgtgcaatg tgctacttgg tctgacctta actaatgatt
     5881 cacagcatgc catgctggcc attccgcttc tggatatcct tttattatta atggtcattc
     5941 cttttcaagc tttagccatg aaacgttaca atgccattcg gggaaccgat gttccttatt
     6001 ttccaaattt gaaagaatta aaacataata tcatggctct agatgaggca gagttacaag
     6061 cctatcataa gacatcgttt gaaagtgtgt tttcattaaa tggagtcatc atcccttcct
     6121 tgtatgtgat tttatttttt gtctatctat tcactggtca agtggagtta acagccatct
     6181 tagttttggt gttaattcag ctctatttac tcgttaagag tgcaacaatg accagacaat
     6241 tttaccgtta gcctaaaagg agactgttta tgaaaggatt cattaattat ttaaaaatag
     6301 cagtgctcat tattctggct atggttttca atgtccttcc gatgatttta ttgcaaaagc
     6361 aacacgatat tcctatggta cttaattggg gaattggtat tttctacttg gttattgttg
     6421 gaagtgtcct tattgtatta tggcgtcttt atcaagctaa gcaagacacc cctattaaac
     6481 agcaaaaaat gagattggtt gactggggtt atttagcatt attttggtta atcatccgtg
     6541 tgatagctat tgtaagtacc cttgttaacc agctatggtc tggtcaacaa gtgagtgcta
     6601 atgatgctgc aatacatacc ttagctagac ttatcaaagg tggtttcccg ctttatactg
     6661 ccctatttgt acttgtgata gcttttatcg ctcctattat ggaagaacta gtctttagag
     6721 gatttcctat gattgatctc ttcaaaggaa aatcacttaa ggtggcaggt ttagtgacct
     6781 ctcttgtttt tgctttacca catgccacca atagtgttga atttatcatg tacagctgta
     6841 tgggcatttt tctctttgtt gcctatcaaa gacgaggaaa cttaaaagat gctatcttgt
     6901 tacatatttt taataacttg attgaagtca ttttgttaat gtcaataggc ttaggagtca
     6961 tataataaaa agcttcggat ggatgaactg caccccaaaa gttggacaaa aaaactaaca
     7021 tttggttgga cttgtcaaga aaaaatagac ataaagttaa gctaatgagt aactattttt
     7081 ttcaaattca acaggtgaga gataccctaa agctgaatgc agacgtttgg ggttatagta
     7141 agtctcacta tatttaaaaa tctcaagctg agcttgctca atagttgcga aatgagcatc
     7201 attgacaagc tctcttttta atgttttata aaaggattcc atgagtgcat tatcgtatgg
     7261 attacctttt cgactcatcc tggacttaca ttttttctgt ctaagtaggt cctgaaattc
     7321 tgctccggta tattgagaac cctgatctgt atggacgatt accccttctt taggcttttc
     7381 tctatttata agcttgattg aaagcttctg ttaccaactt gtcttgcata cgttagccca
     7441 ttgcccaacc aataaccttt ttactataaa cgtctataaa gacagaaaca taaagtattc
     7501 cttcttgtgt aggaatgtag gttaggtctc ctagccataa tttattctta ccagtcgctt
     7561 ggaaacattg attaacaaga ttgggacgag ttaatgaaga acgtcgacgg ttatagtatt
     7621 tatattgata tcgacttcct ttagcataga gacctagctg atgaaggagt ctaccaacac
     7681 gtttatgatt gacatggatg ccacgtctac aaagttcttg ggtaattcgt acgcttccgt
     7741 aacgctcttt atgttcatag aaaatggctt taatcatttc cttcaaagct tcattctcaa
     7801 catgccttga agagggacga cgttttaaat aagcatagaa acctgaacgg gagacttgaa
     7861 gagtttgaca ggtgtgatga ctattcaagc taacactatt ttccttcaga aactgaaatt
     7921 ttatttccgg tttggcttca agaagacctg gaactttttt agaagagcta attcctcctg
     7981 tagcagtttg ttctcttttt caagtttttt tatctcaaat tgagcatgac gaagtgcgct
     8041 cccatgtcct gggaacgcac tttctccata tttttcatat tcttgaatcc atcgataaag
     8101 actattggga tgaatttcta aagttgaact aaccattttt acaggctgct cttcctcaag
     8161 gatgagtttt acagcagaga gtttgaaggc tttatcaaag gtttttcttg gcataatgac
     8221 tattcctcgt tttcacttaa ttttgtgtct actttagtat accaagtcca gccttaagtt
     8281 agacaataaa tctaacttaa ggaagctagc agcatgagaa tccagatggt caagagtgcc
     8341 aaacttaagc tgatgggcac acccagaaga atttgttttt tgaaagcaag actgcgttcc
     8401 tctatatggg gaagggagag ttgaatgaga gaaccagctg atgaaaaagg agagatatta
     8461 gtagatagag cgccaataat agtggctgtt gtgagtaagc gaatatcaat gtgaggactt
     8521 tgagcactga tggtagcaat gatgggaaag atagttgggg ctacaacgga tagggtggaa
     8581 ctaaagagtg acattactcc ggctatcaca caaaagaaca gaggtaacca gaaatgagga
     8641 atggttgttg ttatgaggtg ccctatcagt gtaactaaac ctgacttgac cgctagagac
     8701 attagtaagc tcatgccgca gagcatgata attatagccc agggaacctt agccaaaatg
     8761 gcttcttgct tccctaattt gagccttaag gcgaggcaga ccatgagtat tgagacaaag
     8821 ccaatatcaa atgtttgtcg ataagtagct atccatgcga tgtttgggaa aatgagatgc
     8881 aacaagggaa aaagccaaac caaaaccatg ctgctgatca tgagcaaggt ggtttgtctt
     8941 tggaccttgc tgaggactgg tggttggtca atagtcaagg atgagtttgt tcttccttta
     9001 ctgtagcgga tgtaacaaga taataaaagc aagacgatga gtgggtagat aatgctgacg
     9061 ataaagatat gattgccaag tgaaaaagct tgctcttccc atcccatttg cttaaacagg
     9121 ccttgaaaga caatgcctga gccactggtt atcaaattag cccctcctga agctccccaa
     9181 ttgacggctt gagctccaat caaagggtgt ttgtccgctt tttgacagag ggtaatcgct
     9241 agaggacagc aaacggccat agtagtgaaa aatccagcac ctaaagcaga caaaagggtt
     9301 gctatcaggt ataaaatcat gtagagggcg ttagggtggg tgcgtgtgcg gtagagaatg
     9361 tgttgagcca aaacatcaag agtgccgtta gttgttgcaa cgttataaaa gagagagacg
     9421 ctaaaaatgg taaaaaagag tgaggttggc caaaaatgaa gaagttcttt ggggcttaat
     9481 cccatgagag tggttgcgat gaggtaagaa aaagcaatag ccagcaggcc aatattgatt
     9541 ttggtgcggt aaccaattcc aatggctaga gcaatggcgc taattattat taaatgaatc
     9601 attgaattgt cctttagtta gaatatgaaa ataggttggc aattttggcg ctcaaaagac
     9661 cacctgtttc ctattggtac ttgactatta tacacaaaaa aattacaatt gtcttgacac
     9721 ttgttgggat ttaaaatgag gtattcaggt ttgtgatttc ctacggaagt taatagcaat
     9781 tgcctctagt gcttacaagt gatattaaaa atagaggact tagtgatgtc aatcatttca
     9841 actgatttaa ccccttttca aatagatgat acattgaaag cagccttgcg agaagatgtt
     9901 cattccgaag attacagtac caatgccatt tttgatcatc atggccaagc caaggtgtcg
     9961 ctttttgcca aggaagctgg tgttttagcg gggctaaccg tttttcaaag ggtttttacc
    10021 ctatttgatg ccgaggtgac cttccagaat cctcatcaat ttaaggatgg ggatcgtttg
    10081 actagtggcg atttggtttt agaaatcata ggctcggtga gaagcctctt aacatgtgaa
    10141 cgcgttgcct tgaatttttt acaacattta tcagggattg cttcgatgac agctgcttat
    10201 gtagaagcct taggcgatga tcgcattaag gtatttgata ctcgaaaaac cactcctaat
    10261 ttacgtcttt ttgagaaata tgccgtgaga gttggcggtg gctataatca tcgctttaat
    10321 ttatcagatg ctatcatgct aaaagacaat cacattgcgg cagtaggtag tgttcaaaag
    10381 gcgattgctc aagcgcgtgc ctatgcccct tttgtgaaaa tggtcgaggt ggaagcggaa
    10441 agccttgctg ctgccgaaga agctgcggcg gcgggtgttg atattatcat gttggataat
    10501 atgtcattgg aacagattga acaggccatt actctaattg caggacgttc tcggattgaa
    10561 tgttctggaa atattgatat gaccactatt agccgttttc gtggtttagc gattgattac
    10621 gtctccagtg gtagtttaac ccatagtgct aagagtctcg atttttccat gaagggttta
    10681 acctaccttg atgtctaagt tgcaaaataa actaactttt taaaggatgt ctttcctcta
    10741 gaacgagttt tatgtcagat agtttaaacg ccttttcaaa tactttccct gttttcacct
    10801 gattcgtgtc cgcttattat accaattcca agcattcacc tgaaaaaacg atagggagag
    10861 gttagtgttt ttattgtaaa agttccacgc caaatccatg aagaaaagtt tgttttcaga
    10921 aatcacgatt catcataata aaatcaatca aagtcgttaa ctaggagtgt actgacccca
    10981 aaaagttgga caaataatta ttgaaagg
//
LOCUS       DATURV010000048        10389 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DATURV010000048 DATURV010000000
VERSION     DATURV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.048"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            272..709
                     /locus_tag="VCF75_001271"
     CDS             272..709
                     /locus_tag="VCF75_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546832.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKNSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIAAE
                     TVEESLGQAKEKVEGIGEATSQTIQSKMEKLTSDKTDADDDEK"
     gene            complement(759..1670)
                     /locus_tag="VCF75_001272"
     CDS             complement(759..1670)
                     /locus_tag="VCF75_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP2546833.1"
                     /translation="MKTVRIFYNPNSGKKEDQLAGQVKDYFCQHGFSEDSVEVITPKG
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIKQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(1787..2437)
                     /locus_tag="VCF75_001273"
     CDS             complement(1787..2437)
                     /locus_tag="VCF75_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEP2546834.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(2434..2874)
                     /locus_tag="VCF75_001274"
     CDS             complement(2434..2874)
                     /locus_tag="VCF75_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEP2546835.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQAGSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLSQEVTQ"
     gene            3093..3941
                     /gene="ylqF"
                     /locus_tag="VCF75_001275"
     CDS             3093..3941
                     /gene="ylqF"
                     /locus_tag="VCF75_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEP2546836.1"
                     /translation="MAMIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFREYY
                     PNRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GNMDAD"
     gene            3931..4722
                     /locus_tag="VCF75_001276"
     CDS             3931..4722
                     /locus_tag="VCF75_001276"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEP2546837.1"
                     /translation="MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKA
                     IQAELAEEERLEAMLSYEKALYKKGYQAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            4787..5623
                     /gene="dprA"
                     /locus_tag="VCF75_001277"
     CDS             4787..5623
                     /gene="dprA"
                     /locus_tag="VCF75_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEP2546838.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQKQQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQKLKIEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVITEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            5730..7859
                     /gene="topA"
                     /locus_tag="VCF75_001278"
     CDS             5730..7859
                     /gene="topA"
                     /locus_tag="VCF75_001278"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEP2546839.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(7934..8818)
                     /locus_tag="VCF75_001279"
                     /pseudo
     CDS             complement(7934..8818)
                     /locus_tag="VCF75_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8831..9376
                     /locus_tag="VCF75_001280"
                     /pseudo
     CDS             8831..9376
                     /locus_tag="VCF75_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            9391..10383
                     /locus_tag="VCF75_001281"
     CDS             9391..10383
                     /locus_tag="VCF75_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEP2546840.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESPLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGRKVVGYDIYQSDAAKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNS
                     DLFKSFKKGAILMNMARGAVIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
BASE COUNT         3387 a         1820 c         2088 g         3094 t
ORIGIN      
        1 gtcactttga caaccattta ttggtccttt tagataagac aattttgtgg ctcttcagtt
       61 gaaaggatgt gttgttgatg ttagaggaaa caatcatgtt aaattaactc aggcttaaag
      121 accaaaaaag gagtgaagtc tattatgaaa aaaagaaatt ttaaacttac agaaatgtta
      181 caattggttt tattagtggt tcctgctgta aaacttgcta aaaaaatgtt taacgatagt
      241 aaaacaccgt ctaaaagaaa gtaggtctac aatgactaaa aaaaatcatg gcgttttttg
      301 ggggttgttg cttggatcag cagcagctag cattgcttac ttaagtttat cgtcttccaa
      361 aaaagatcaa ctcttgaaaa atagtgctaa aaaaatagat gatctcaacg cttacttgca
      421 agataaaagt aagcaagttc tggatgcagt atcggaaaaa gtacaagaat ccaaagatgc
      481 tgttgaagtt tatggtggta ttgccgcaga aaccgttgaa gaaaagtggc aagataccaa
      541 agatgctgtt gaagtttacg gtggtattgc cgcagaaaca gttgaagaat ctttgggaca
      601 ggctaaagaa aaggtagaag gaattggcga agctactagt caaactatcc aatctaaaat
      661 ggaaaaactc accagcgata agacagatgc tgatgatgat gaaaaatagg ttttaaaata
      721 cctttaagat aagacaaaaa gtgccattag gcactttttt aatcttctgt tggaacaata
      781 atcgaaatgg cttttggaat gatatctaat tgaatgggca gatagtcact tttatcccca
      841 tcaatcctgg tacgtggtaa tatcgcttgt tttttagctt gtggtagtaa gctaagcgta
      901 ttggcttgaa aatgactgat ttcctctgct ttggaaaaat caccctgttt aaaatcatta
      961 atatgccata aaaatttaaa gaaagagact tttttcatgg tataaacttg gaaatagcca
     1021 tcgtcagctt gagcaccagg agaaaaagaa ggaaaaccag caatggtatt tgtcatcgtg
     1081 attaagagaa acttcgtttt taggtgaata cgatggttgt ggctaattaa agagatggca
     1141 tagctgcgat ttcgtttcag tattcgaata ctatctttta aaaatgctag aggaccaaag
     1201 cgtcgttttt cctcagccgt tacgtcggca gcaatatcag ctagtaatcc taaggttaaa
     1261 ctggaaatca tgtattgctg gttagctttg caaatatcaa cttgcttgat ttgtccagtc
     1321 aagatagtat ccaacgcctc agtaatttgt aaagggatat gcatagcctt ggcaaaatta
     1381 ttgactgtcc ctgagggcac taagcctatt aaacaatgag ctcctccttc atagatgcca
     1441 ccgatgattt tatttagtgt cccatcgcca ccaagaggga taaccaagtc aatcttgtcc
     1501 ttagcagctt gttttgctaa ttgaaaagcc tggtcagcac ctttaggggt aataacttct
     1561 acagaatctt cggaaaaacc atgttggcaa aaatagtctt tgacttgccc agcaagttgg
     1621 tcttctttct ttccagaatt aggattgtaa aaaatccgaa cggttttcat caggtctctc
     1681 ctttgacagc ttattacttg ctatccatta taccacttct aagaattgat gtcttacacc
     1741 aactcatcac attaagcctc tcaatcagtc tgagcatcta tttatcttaa agcataaaga
     1801 aagtgatagc gataaattgg agggcagagg ccaataaaat aaagagatgc caaatcatgt
     1861 ggaagtaggg tctttttttg gcataaaata ccgctccaat ggtataagac aagccaccaa
     1921 ataacatgag aagaccaaaa gcaagactag ttttttgaat gatgactggc aagataaaca
     1981 caacgagcca gcccataacg atataaagca tcaaactgaa tttttcattg atacgtttgg
     2041 caaaaatttt gtataaaatc ccaaaaagcg taatgcccca ctgcaaaaca ataatgatat
     2101 agcctagcca accagaaaca agggataagg caacaggtgt ataacttcct gcgatagcaa
     2161 tgtaaatcat actgtgatca ataatgcgta gaatgtattt gtgaaccgaa ccataagcca
     2221 tggagtggta aatcgtagag gacaaaaaca tcaaaaagag actgatgaca aagatggaga
     2281 taccaatagc ggctttaagg tcataagttt gataagcata gctggctgaa ataggaagta
     2341 gaatgagcat agcaaatgct ccgatagcgt gtgtcacgct gtttgctact tcctcactaa
     2401 aagataaggg taaactttgt ttaaaggttt ggttcattgg gtcacctcct gtgataaata
     2461 aatagttttg tggtaatctt tgaccgtttg acaggcaacc tgcacaatcg gtggaatgtc
     2521 ctcctcaatg ggagctttct gattccaaca ggttacaaaa gtattgtaga gactttcaga
     2581 accagcctgt gcctgaagtt cctccaatac gcgactaata tcttgaatag gaaataccgt
     2641 tttaaacaaa ctgatggcaa ttaagcgcgc tagttgttgc ttttggtatt ttttcttgat
     2701 aggtttagtc acatacccat gcttgacgta attattaatc atagatgccg ttagtgattt
     2761 attatctgac acttctgaaa aatcagtgca ctggttgaca taaagtaaca cttgatccaa
     2821 atataagtct aaatcaggga ggtccttcca ataaggtaat gatggaaaaa tcatagtttc
     2881 gtctctctat ctagttttga taactagatt atattattaa tgaaaactta ttgcaagcaa
     2941 aaactgtaaa gacttaaaag aggaaagtag aaatggcgaa gtgaagctaa tcatgctata
     3001 atgttaaaga attgattggg atagatgcct tctgtcgcaa tcagactatt ttatcacgaa
     3061 gaagatgatg ctacgaaagg aatgatacac tcatggcaat gattcaatgg tttcctgggc
     3121 acatgtccaa agccagaaga caggttcagg aaaatgtaaa gcatgttgac tttgtaacta
     3181 ttttagtgga tgcacgctta ccactttcta gtcaaaatcc catgcttaca aaaattgttg
     3241 gtgataaacc taaattaatg attttaaata aagctgactt ggcagatgca actcgaacta
     3301 aagagtggaa ggcttattat gaaagtcaag gtattaaaac attagccatc aattccaaag
     3361 aacaatcaac ggttaaaaaa gtaacagaag cagccaagga actcatggct gataaaattc
     3421 aacgcttgcg agagcggggc attcaaaaag aaaccttgcg aaccatgatt attggcattc
     3481 caaatgctgg taaatctacc ttaatgaatc gcttggctgg taagaaaatt gctgtagttg
     3541 gcaataaacc tggtgtgact aaaggacaac aatggttgaa gtccaataag gaattggaaa
     3601 ttctagacac tcctggtatt ttatggccaa aatttgagga cgaattggtg ggcttaaaat
     3661 tagctttaac aggtgctatc aaggaccaat tactaccaat ggatgaagtg actatctttg
     3721 gcttgaatta ctttagggaa tactacccta atcgtttaac caaacgtttt aagaatattc
     3781 ctcttgagga agaagctcct gaaatcatta tgaccttgac acgccaactt ggatttaaag
     3841 acgattatga ccgtttttac actttattcg ttaaggaagt tcgtgatggt aaattaggtc
     3901 aatacacctt agatcaggtg ggaaatatgg atgccgacta gtattaaagc tattaaagaa
     3961 agcttagagg ccgttactag cctcttggac cccctctttc aagaattggc aaccgacact
     4021 aggtcaggcg tccaaaaagc tctaaaaagc cgacaaaagg ctattcaggc cgagttagca
     4081 gaagaagaac gattagaagc catgctttct tatgaaaaag ctctttataa aaaaggttat
     4141 caagccattg caggtattga tgaggtggga cgtggtccct tagcaggtcc cgttgtggca
     4201 gcttgtgtga ttttacctaa gtattgtaaa attaaaggcc ttaatgattc taaaaaaatc
     4261 cctaaagcta agcatgagac catttatcag gcagtgaaag aaaaggcttt ggctatcggt
     4321 atcggtatta ttgacaatca gctgattgat gaggtcaata tttatgaagc aaccaaacta
     4381 gctatgctag aagccattaa acagttggag ggccaactca cacaaccaga ttatctcttg
     4441 attgatgcca tgacattgga tattgctatt tcgcagcagt ctattcttaa aggcgatgcc
     4501 aattccttgt ctattgcagc agcatcaatt gtagctaagg tcaccagaga tcagatgatg
     4561 gctaactatg atcgcatttt tcctggttat ggcttcgcta aaaatgcagg ctatggcacc
     4621 aaagaacatt tacagggatt aaaagcttac ggcataacgc ctatccatcg taaaagtttt
     4681 gaacctgtta aatccatgtg ctgcgattca actaatcctt aaaaagttct gtttttagca
     4741 gaattttttt cttttttacg aataaatgaa gtaaggaggt aacttagtga atcattttga
     4801 actttataag ctaaagaaag ctggactgac taataaaaac attctcaata ttcttgacta
     4861 ccaaaaacaa caggaaaaat cgttgtcact tcgagatatg gccgttgttt ctggctgtaa
     4921 gcatccgtct cactttatag aagcctataa gcagttagat attcaaaaat taaaaataga
     4981 atttaaacaa tttcctagta tttctatctt agataagcat tacccaatgg cattgaaaga
     5041 aatatacaat ccacctgtcc tcttgttttt tcagggaaat ttagaccttc ttgagaaacc
     5101 taaattagcc attgtcggct ccagacgctc aagcgatacc ggagtaaagt ctgtccgtaa
     5161 aattcttaaa gaactcggga accgttttgt gattgttagc ggacttgctc gaggtatcga
     5221 cactagtgct catttagcct gccttaaaaa tgggggacaa accatcgcta ttattggaac
     5281 agggttggat cgcttttacc ctaaagaaaa tagggagttg caaactttct tagggaaaaa
     5341 tcatcttgtg ataacagaat acggtccagg agaagaagct ttatcttatc actttccaga
     5401 acggaatcgt attatcgcag gccttagccg aggtattctt gtcgttgaag caaaaaatcg
     5461 ttcaggttcc ttgattactt gtcaaattgg gatagaagaa ggccgagaca tttttgctgt
     5521 cccaggaaac attttggacg ggaaatccga aggttgcctt cagttaatta aagagggagc
     5581 aacatgcgtc acatcgggca tggatatcct ttcagagtac taaaaaagtt aaagtttctt
     5641 cctatatagc atttttttat tgacagtttc ttaaaaatgg gatattatgc tataagcttt
     5701 aaacaaactg ttataggaag tgtgtcgaat tgggaacaaa aactacaact aagccaaaaa
     5761 cgggtactaa aaagtccacc actaaaaaga aatcaacagc gaaaaaaaat ctcgttattg
     5821 ttgaatcacc tgccaaagct aagactattg aaaaatatct tggacgcagt tataaagttg
     5881 ttgcttcggt aggacatatt cgtgacttaa aaaaatcgtc tatgtctatt gattttgata
     5941 ataattatga gccacaatac attaatatcc gtggtaaggg accccttatc aattctttaa
     6001 aaaaagaagc caaagctgct aaaaaagttt atttagctag tgacccggat cgtgagggag
     6061 aggctatttc ttggcatttg tcacatatct taggacttga tcctcaagat aataaccgag
     6121 tggtttttaa tgaaattacg aaagatgcag ttaaacacgc ctttgtggag cctcgtcaga
     6181 ttgatatgga cttggtagat tctcaacaag cacgtcgtgt tttggaccgt attgtgggat
     6241 attccatttc accaattctt tggaaaaagg tcaaaaaagg actttcagca ggacgtgtcc
     6301 agtcggttgc tttaaaactc atcattgacc gtgaaaatga catcaaagca tttgttccaa
     6361 aggaatactg gtctattgat ggtcttttta aaaaaggaac taaaaagttt caagcgactt
     6421 tttacggtat taatggcaaa aaaacaaagc ttgataataa caacgatgtc aaagaggtat
     6481 tagcaaagct aacaaatgaa gattttcttg tcagcaaggt tgacaagaaa gagcgacgtc
     6541 gtaatgctcc cttaccttac acaacatcat ctcttcaaca agatgctgct aataaaatca
     6601 atttcagaac gcgtaaaacc atgatggtag cccaacaact gtatgagggg attcatttag
     6661 gagaaaatgg aactcaaggt ttaattacct atatgcgtac ggattctact cgtatcagtc
     6721 cagtagcaca aaacgatgct gctcaattta ttatcaatcg ctttggagct aattattcaa
     6781 aacatggtaa tcgtgtcaaa aataccagtg gtgcccaaga tgctcacgag gcgattcgtc
     6841 catctagtgt taatcatacg ccagactcta ttgccaaata ccttaacaag gatcagttaa
     6901 aactttatac gcttatttgg aatcgttttg tggctagcca aatgacagca gctgtttttg
     6961 ataccgtaaa ggtgaacttg gagcaaaatg gcgtgatttt tgttgccaat ggtagccaaa
     7021 tgaagtttga tggttacatg gctgtttaca atgattccga taaaaacaaa atgttacctg
     7081 agatggcaga aggagaaacg gttaaaaaaa tatcgacctc tccagaacag cactttacgc
     7141 agccaccagc tcgttattct gaagcaactc tgattaaaac gctagaagaa aatggtgttg
     7201 gacgtccatc aacttacgct ccaaccttag aagtcattca acgcagatac tatgttaaat
     7261 tgtctgctaa gcgctttgag ccaactgagc ttggggaaat tgtcaacaag cttattgtcg
     7321 aatttttccc agatattgtt gatgttgcct ttactgccga gatggaaggt aagctagatc
     7381 aagttgaaat cggagaagaa caatggcaac atgttatcga tcaattctat caaccctttg
     7441 tcaaagaatt gaataaggct gagtctgaaa ttgaaaagat tcaaatcaaa gatgaaccag
     7501 ctggcttcga ctgtgatgtt tgtggtcacc caatggtcat taagttagga cgttttggga
     7561 aattttacgc gtgtagcaac ttcccagagt gccgtaatac aaaagccatc actaaagaaa
     7621 ttggtgttac ttgtccggtt tgtcataaag gacaagttat cgagcgtaaa accaaaaaaa
     7681 ataggatatt ttatggttgt gatcagtatc cagattgtga gtttatctcg tgggatttac
     7741 ctattggacg tgcttgccct aagtcaggtg actatcttat tgagaaaaaa gttcgtggtg
     7801 gcaaacaagt aatgtgtagc aatgagacct gtgattacaa ggaagaaaaa attaaataaa
     7861 aaaagaattg gaactccttg tcttgttcca attctttttt tatttacaaa ggtattttta
     7921 actacaaaaa tcgctagttc gtttttaaaa ggcttaaaaa ttcctcaata actggactga
     7981 aactcgcatc atctcggtaa gcaatcccaa tagggtaaaa attattttta tcttgcaaag
     8041 gaatccaaac taagttatct acctgattaa gactcttaaa atcactcggg aggatagtca
     8101 aggtatttaa atcttgcaaa ctatgaataa gaacttccca atcattgtgt ttaaagacaa
     8161 tatctggttc aaaaccgaga gctcggcatt ttctaggaag catttcgcct agcatgtagt
     8221 gatcgtttaa agaagcaatc ttatagcctt ttaaatcttt aagttttaac tgaggatgtt
     8281 gcgccaaagg gtgctgtttg aggagaacga tactgacctt atagcctttt gtggacgttt
     8341 gtagtagctc aatggtaata tccttttgaa tacttagaaa ggagaggaga ccaatatcga
     8401 tttttccttg ggacaataat tcttataatt tgcgagaacc atcctgtata agcgataatt
     8461 caacatttga gtgagtagcc ataaagattg aaatttgttt catgaattgt atagcaaaca
     8521 gtgatgttaa ttcaacacga atcatctctt tttttcttga tttaattgct gaatatccgc
     8581 aacaatttga ttaaagtcat ccacaaggtc tttgcctttg tgataaagaa tatctccagc
     8641 ttcggttagt agtagctgtc aatcactttg tgtaaaaagc gtcgtgttca aactaggact
     8701 ctagcctttt aattgattga gaaagagtat gttgcgttac aaacaaactt ttagcagcgt
     8761 gggaatgatt ttttgtttca gcaactgcaa taaaataggt taattatcta atatccatag
     8821 tagatccttc atgattggtc agggaattgc gtctctactt ggtttacaac ctattttgat
     8881 gtctctcctt attgccatga ttttctgctt ccttattgtt tctccaattg caacggtagg
     8941 aattgctctt gcgattaact tatctggtat tggttcaggt gccgcaagct ttggactttg
     9001 cttagcaggt tgggctgtta attcaaaagg aacttcactt gctcacgtct tgggatcacc
     9061 gaaaatttca atggccaacg ttttatcaaa accaaaaatc atgttgctaa tgttatgttc
     9121 tgcagctgta cttggtgtta ttggtgctat ctttaatatt caaggaacac ctgctagtgc
     9181 aggctttgga attagtggtt tgatcggacc tatcaatgcc cttaatttag ctaaaggcgg
     9241 atggtctcca gtaaatattc tacttatcat tgtcattttt gtaggggcac caattgttct
     9301 caatatgatc tttaactacc tttttatcaa ggtgcttaaa gtgattgatc caatggatta
     9361 caaacttgat atctaagaaa ggaacttttg atgaaattaa aattatataa tgttagaggc
     9421 gaagaagcag tcttagcaaa aaaatgggct gatgacaatg ggattgagat ttccttgacg
     9481 gagtctccct taactcctga aacggttaaa gaagctgaag gctttgacgg gattgccaat
     9541 gcccaaattg gtcctttaga tgatgcgatt tatcctctct taaaggaaat ggggattaaa
     9601 caaatcgctc agcgcagtgc tggtgtcgat atgtataacc ttgatttggc caccgaaaat
     9661 gacattatta tcacaaatgt tcctagctat tctccagaat ccattgcaga atttacggtt
     9721 actattgccc ttaatttaat tcgtcatgtg gagttgattc gtgaaaatgt taaaaaacaa
     9781 aatttcactt ggggactccc tatccgcggc cgtgttttag gtgatatgac agttgcgatt
     9841 atcggaactg gacgtattgg tctagctact gctaaaatct ttaaaggttt tggccgcaag
     9901 gtcgttggat atgatattta ccaaagcgat gctgccaaag ctgtcttgga ctataaggaa
     9961 tctgtagaag aagcgattaa agatgctgat cttgtttcct tacacatgcc accaactgca
    10021 gagaacacac accttttcaa ttctgattta tttaaatcat tcaaaaaagg ggctattttg
    10081 atgaatatgg cgcgtggtgc cgtcattgag acgcaagatt tactggatgc tttggatgca
    10141 ggcttactga gcggagctgg tattgacact tacgaatttg aaggacctta tatacctaag
    10201 aattttgaag gtcaagaaat tacagattca ctatttaaag ctttgattaa ccatcctaag
    10261 gttatttata cgcctcatgc agcttattat actgatgaag ccgttaaaaa cctagttgaa
    10321 ggtgccctta atgctacagt agagattatc aaaactggga caacgacaac tcgtgttaac
    10381 taacaactg
//
LOCUS       DATURV010000049         9758 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DATURV010000049 DATURV010000000
VERSION     DATURV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.049"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            10..675
                     /locus_tag="VCF75_001282"
     CDS             10..675
                     /locus_tag="VCF75_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEP2546841.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     ADD"
     gene            668..1383
                     /locus_tag="VCF75_001283"
                     /pseudo
     CDS             668..1383
                     /locus_tag="VCF75_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
     gene            1529..2875
                     /gene="trmFO"
                     /locus_tag="VCF75_001284"
     CDS             1529..2875
                     /gene="trmFO"
                     /locus_tag="VCF75_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEP2546842.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAKSVTAELENHPLIEVIRDEITEIPNDAITVIATGPLTSDALAEKIHAVNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            3028..4434
                     /locus_tag="VCF75_001285"
     CDS             3028..4434
                     /locus_tag="VCF75_001285"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEP2546843.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQARQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            4477..4791
                     /locus_tag="VCF75_001286"
     CDS             4477..4791
                     /locus_tag="VCF75_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546844.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            4791..5141
                     /locus_tag="VCF75_001287"
     CDS             4791..5141
                     /locus_tag="VCF75_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEP2546845.1"
                     /translation="MKRYEVTVNGQVYEVSLRELADGETVEVSQPAAPATEKEMNANA
                     AGGGIQVKTPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            5151..6281
                     /locus_tag="VCF75_001288"
     CDS             5151..6281
                     /locus_tag="VCF75_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEP2546846.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKVMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKVGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(6482..7366)
                     /gene="citG"
                     /locus_tag="VCF75_001289"
     CDS             complement(6482..7366)
                     /gene="citG"
                     /locus_tag="VCF75_001289"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEP2546847.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGCFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(7359..8054)
                     /locus_tag="VCF75_001290"
     CDS             complement(7359..8054)
                     /locus_tag="VCF75_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2546848.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKKTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(8212..9615)
                     /locus_tag="VCF75_001291"
     CDS             complement(8212..9615)
                     /locus_tag="VCF75_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEP2546849.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
BASE COUNT         3004 a         1927 c         1988 g         2839 t
ORIGIN      
        1 gggactctta tgacatatat agcgcttatt ggagatatta tccagtccaa acaattgacg
       61 gatcgttcca aagttcaaaa aactttagca gcttatttag acgacctcaa caaaaccttt
      121 gctccttata ttatttctaa attatcccta acgttgggag atgaatttca aggacttttt
      181 caggtggata ctcctatttt ccatctgatt gatttgatta atcatcacat ggatatccct
      241 attcgatttg gagtaggagt aggttctatt ctaacagaca tcaatcctga tattagtatt
      301 ggggctgatg gacctgctta ttggcatgct cgtgaggcta ttcgttatat tcatcaaaaa
      361 aatgattatg gtaatactac attagctctt cggactggtc atcacaacca ggatgatgta
      421 ttaaacagtt tactagcagc tggagatgct attaaagcca actggcgagc tagtcaatgg
      481 gaaatttttg acacgctttt agatttgggc atttatgagg aatactttga tcaacaacga
      541 ctaggaaagc aattatctct tagctctagc gctttgtcaa aacgtctaaa aagtagtcat
      601 gttaaaattt atctgagaac acggcagagt gcgctaaact gtctcaaaca aatcaaggag
      661 gaagctgatg attaatggtg tctctcacta cctagctcaa acccctacac ttaccctgtt
      721 tctcatttgt catgtgttat cagactacca gttacaaagt cagcaagtag ctgatttaaa
      781 agaaaaacat ttgacttatt taggttacca cttgattgga gttagcattc ccctaatttg
      841 tttaaccctc attatcccac aagcatggtt gatgagccta ttggttatga taagtcacgc
      901 gcttattgac tggctaaagc caaagatggc taactctcta aaatggaaaa gagagtggat
      961 ttttctgctg gatcaatgct tacatatcgc tattagttca tttgctgggc taaggttagc
     1021 agggatgacc ttgcctaatt ggcttccgat atctattttg atgaccgttt tatttatcct
     1081 actcattacc aaaccaacta atattgtctt taaactcttt ttcagcaaat accagcctga
     1141 ccaaggagaa aaaatggaca cgatcatagg agcggggcta ccataggaat tctagagaga
     1201 attgttattg gggtgtgtat gatcatgggg caatttgctt caataggtct ggtctttacc
     1261 gctaaatcta ttgctcgcta taataaaatt tcggaaagtc cagcttttgc agaatactac
     1321 ctaattggtt ctttatttag tattctcagt gtttttatcg cagcatggat ttgtttcttc
     1381 tagttgaaaa gggttcccgt agaaagaggc tacacatagc ctcttttgct tttttcccct
     1441 ttctgttata ataagtcagg taactacatt agaaagattt gtgggtctgt catctaagag
     1501 atgtcattcc cgaagaggta ttagtgactt gtctcaatca actgcaactt atattaatgt
     1561 tattggagct ggactagctg gttctgaagc tgcctatcag attgctaagc gcggtatccc
     1621 cgttaaattg tatgaaatgc gtggtgtcaa agcaacaccg caacataaaa ccactaattt
     1681 tgccgaattg gtctgttcca actcatttcg tggtgatagc ttaaccaatg cagtcggtct
     1741 tctcaaagaa gaaatgcgtc gcttggattc cattattatg cgtaatggtg aagctaatcg
     1801 tgtacctgct ggaggagcaa tggctgttga ccgtgagggg tatgcaaaga gtgtcactgc
     1861 agagttggaa aatcatcctc tcattgaggt cattcgtgat gaaatcacag aaattcctaa
     1921 cgatgccatt acggttatcg cgacgggacc gctgacttcg gatgccctgg cagaaaaaat
     1981 tcacgcggta aatggtggcg atggattcta tttctacgac gcagcagcgc ctatcattga
     2041 taaatctact attgatatga gcaaggttta cctcaaatct cgctacgata aaggcgaagc
     2101 tgcttacctc aactgcccta tgaccaagga agaattcatg gctttccatg aagctctgac
     2161 aaccgcagaa gaagccccgc tgaatgcctt tgaaaaagaa aagtattttg aaggctgtat
     2221 gccgattgaa gttatggcta aacgtggcat taaaaccatg ctttatggac ctatgaaacc
     2281 cgttggattg gaatatccag atgactatac aggtcctcgc gatggagaat ttaaaacgcc
     2341 atatgccgtc gtgcaattgc gtcaagataa tgcagctgga agcctttata atatcgttgg
     2401 tttccaaacc catctcaaat ggggtgagca aaaacgcgtt ttccaaatga ttccagggct
     2461 tgaaaatgct gagtttgtcc gctacggcgt catgcatcgc aattcctata tggattcacc
     2521 aaatctttta acggaaacct tccaatctcg gagcaatcca aaccttttct ttgcaggtca
     2581 gatgactgga gttgaaggtt atgtcgaatc agctgcttca ggtttagtag caggaatcaa
     2641 tgctgctcgt ttgttcaaaa gagaagaagc acttattttt cctcagacaa cagctattgg
     2701 gagtttgcct cattatgtga ctcatgccga cagtaagcat ttccaaccaa tgaacgtcaa
     2761 ctttggcatc atcaaagagt tagaaggccc acgcattcgt gacaaaaaag aacgttatga
     2821 agctattgct agtcgtgctt tggcagattt agacacctgt ttagcgtcgc tttaacacta
     2881 aaaaagagtt ccttttcagt tgagttgctg aagaggactt ttttatttgc tgatttgagc
     2941 gctagctatg caaaagaatg cacaaaaaat acatgaatag actatggtca ctagaagcaa
     3001 tattatataa tgaatagtac tcattagttg attcagtttg gaggttttag catgcagcag
     3061 caagtcgcaa taacagaaac agttttacgg gacggtcatc aaagtttgat ggcaacgcgt
     3121 ttatctattg aggatatgct tcctgtcctt accatcttag acaagattgg ttattattcc
     3181 ctagagtgtt ggggaggagc aacctttgat gcctgtattc gttttttgaa tgaagaccct
     3241 tgggaaaggc tgcgcacgtt aaaaaaaggc ttacctaata cccgtttaca aatgttatta
     3301 agaggccaaa accttttagg ctaccgtcat tatgcagatg acatcgttga caaattcatt
     3361 agtttatcag ctcaaaatgg gattgatgtt ttccgcattt ttgatgccct aaatgaccca
     3421 cgaaatatcc agcaagcctt aagagctgtg aaaaagacgg ggaaagaggc tcaactgtgt
     3481 attgcctata caactagccc agtgcacacg cttaactatt acctttcttt agtgaaagaa
     3541 ttggttgaga tgggagctga ttctatctgc atcaaagata tggctggtat tctaactccc
     3601 aaagcggcta aagagttggt atctggtatc aaagccatga ctaacttacc tcttattgtg
     3661 catacccatg caactagtgg catttctcag atgacctatc tagcagcagt agaagctggt
     3721 gctgatagaa ttgataccgc tttgtcacct tttagcgaag gcacttcaca acctgctaca
     3781 gaatccatgt atttagcctt aaaagaagca ggttatgata tcacattaga tgaaacgcta
     3841 ctggaacagg ctgctaatca ccttcgacaa gcccgtcaaa agtacttagc agatggcatt
     3901 ctggatccca gtcttctctt tccagaccca aggacactac aataccaagt tcctggaggc
     3961 atgttatcta atatgctatc tcagctgaaa caagccaatg ccgaatcaaa attagaagaa
     4021 gttttagcag aagttcctcg ggtaagaaaa gacttgggtt atcctccctt agtaacaccg
     4081 cttagccaaa tggtcgggac tcaagctgct atgaatgtga ttttaggaaa accttaccag
     4141 atggtgtcta aggaaattaa acaatacttg gcaggcgact atggcaaaac accagcacca
     4201 gtcaacgaag acttaaaacg aagtcaaatt ggctcagctc ctgtcaccac aaatcgtcct
     4261 gcagatcaac taagtcctga attcgaagtg ttaaaagcag aagtagcaga tttagcccaa
     4321 acggacgaag atgtgctgac ttatgccctc ttcccaagcg tagcaaaacc gtttttaaca
     4381 accaaatacc aaactgatga cgtcattaaa gtgactgcct ttatcaaagc ataataggct
     4441 atcttaatca gatagaaaca aaaatatgga gatcaaatga cctattcctt tattgatatc
     4501 ttacaagtta ccttagtatc cttgttctta gtgttaacct gtttaacagg gattatggga
     4561 ttaatgatgc taagtggtaa attattgtca caagaaaaaa aaactctctc tcaacaagct
     4621 tccagcattc ctcaagagca agaagctgac aagttgagtg gagaagctct cgaaactctt
     4681 ttagccaaag acgaattggc tagaattgct gttatgacta gtcttgctta tgctagccaa
     4741 gaagagtcag gaaaacggtt tgaagttatt gccgtggaaa ggaaatccta atgaaaagat
     4801 atgaagtgac tgtcaatgga caggtttatg aagtgagttt acgtgaacta gctgatggag
     4861 aaactgttga agttagccag ccagcagcac ccgctactga gaaagaaatg aatgccaacg
     4921 ctgctggcgg tggcattcag gtaaagactc caatgtctgg aacagtcttg tctatctttg
     4981 ctacagaagg aaaagctgtt aaaaaaggag aagctgttct ggtcttagag gccatgaaaa
     5041 tggagaatga aattctagca cctgcagatg gtctggtttc aaaaattcat gttgtggcta
     5101 accaaacggt cgagtctgaa caagttttga tttcattcta ggaggaaacc atgctagacg
     5161 tactcaatca aatggtccaa tcgtcaggat tggctcatct aactgttaat aatcttatca
     5221 tgatttgctt ggcctgtttc tttttatacc ttggtatcaa aaaggaatat gagccttacc
     5281 ttatggtacc aatcgccttt gggattttac tcgttaattt gcctatggcg ggattgatgg
     5341 accaccctgc caatggtaat cctggtggcc ttctttatta cctatataag ggaactagcc
     5401 tagggattta cccaccttta atttttcttt gtttgggggc atcaactgac tttggtccct
     5461 tgattgccaa tcctaaaacc attttactag gaggcgcagc acaagttggg attttcttag
     5521 cctttttcct tgctattatg ttaggcatga cacctcaaga agcggcttct gtcgggatta
     5581 ttggtggtgc cgatggacca acagctattt atgtgacgac aaagttggcg cctgatttac
     5641 tctctactat cgccctagca gcttattctt acatggctct ggtacctatt attcaacctc
     5701 ctatcattaa actcttgact accaaggcgg aacgccaagt gaaaatgact caagcaagaa
     5761 ctgtttcaca aaaagaaaaa atcattttcc cgattatggt aacaatcttt gtcagccttt
     5821 tggttccaag tgctacaacc ttggttggct gtttgatgct gggaaatctg gtcagagaaa
     5881 ttaaaattgt tcctaaaatt gtagagaatc tccaacaagt ggttatgttt tgcattacta
     5941 tcattttagg gttaaccgtt ggtgctaagg ccaatggaga tctcttttta tcagccacaa
     6001 ccttgaaaat tattgccctt gggctaatcg cctttgctgc tggtacagca gggggtgtct
     6061 tgatggggaa ggtcatgtac tatttatctg gtaacaaagt gaaccctatg ataggagcag
     6121 caggtgtttc agctgttccg atggcagcgc gagtggtcca aaaggtcgga caagaagaag
     6181 atccaagtaa tttcttactc atgcacgcca tggggcccaa tgtagctggc gttattggct
     6241 cagccattgc ctcaggtgcc ttgctggcct tctttggcta attgtgaaaa aggattagta
     6301 aattaatctt aataaatcat tatcaggagt tggaagcaat ctagctcctt ttttagtaga
     6361 aagctcgagt gataaactat tccaaaataa ttataaaaaa agactgtcct gattgtacgg
     6421 actatatcaa ttgactttta tagtctagct gaaatagcag tgcaaaacta agacactcgt
     6481 tttaaagtag tttttctaaa aaagcaaaat aaaaggtcag agccaataaa tctgccgcgc
     6541 ccccagggct taaatgctga ttaatgaggc attgattgta ggaggacaaa gccaatctta
     6601 ggtcagttgt tgataaatct tgttgcagga gtagtcgcat atctgcttta acgcctttcc
     6661 atgcttcaat ccctccacga tgaatcagat ttccatcttc aacaatagac atcaaataaa
     6721 caagaagacg aagctgactg gtttcggggt cattttgact tatcatctga cgaaagtagg
     6781 gcaaggcatg gtctgttaag gtcgtgaatc cctcgcttgc ctcaccccga ggtcctttga
     6841 tgccataagt tacaaagagt tgctccccat aagtgaactc ttttttagtg ttcaaatgac
     6901 caaggtctgt ttgaatcaaa tgtgccgtca taggttttac taaacgacag atagctagag
     6961 tatcttcctt gctaaaacac cctaaatcag tcattaagtg aggtgttctt gctagatagg
     7021 ccccagttgc accaagaagt aaagccatgg aaaaattcag gcctttatga gtgttaattc
     7081 cctgagtagc acaaaacatg gtttcctcgg ccttttgacc tagttgtcta agacggttaa
     7141 acagaagaag tggctcttct tttgcataag caaaacctgt ttcgatataa gcctgaaaaa
     7201 aaggagagag tgctatcata ctgtcgataa aggtcataaa ggacatgtca tcatgggcac
     7261 cgttatcaaa tcgatcaact aaaccaggtt ttggagatag tgagacttca taaagcaaag
     7321 cttttaaggc cagttgacta atacttgtta gaactgcctt agtcattttg ctcaggcctc
     7381 atttctttta agataaattc aagtgagcta ttcaagtgtt catgtgtaat caacgtaatt
     7441 tgctcatgat ctttgttctt catacctcgg taaatcatcc agtgttcaga tagagctcgt
     7501 ttacggcgtt caactgaaga aatcgaaatt tttctaaagt agaccaaata agcttgcaat
     7561 tctgtcacaa tttcttttaa gcggaccatt tggctatagc tgtaaatcaa attgttaaag
     7621 gcattaaaat tatccaaaat acgattggtg tcatcctctg cgataaaggt ttcacagtct
     7681 tccaacaagt tatgcataac tttaaaatct tcttctgtca ttaaatgcat cgcttgcact
     7741 gttgcgagag tctctagagc ttttcgaatt tcaaaaattt cacaggcatc cttgatacta
     7801 actcctttga caataatgcc ttttttagga atgtgctcta caaggtgttc ttcagacaat
     7861 aatcccaaag cgtagcgaat aggtgtcctg ctaatattaa gcgcaatgga aaactctttt
     7921 tcattaatgc ggcttccaac aggaatttgt cgcaaaataa tggtcttttt aagagcgtta
     7981 taaaaggcta ttttcaaagg gatatttctt gataggtcaa gattttgttt aacggcttcg
     8041 ataataggat tcatgtcatc tccttaatgc tagtgtcctc actttattat acttaaattt
     8101 gtgaaacatt ccaataggac tagatgagtt ctgaaaaatt aaagccatgc tcacaaaagg
     8161 acatgacttt aatcatctca taaggaataa ggtagtagtg ttatcgttgg tttattgtgg
     8221 aatgtaaagt ggcatttgcc ccatggcaat aatggttacc acaaagataa caaagatgat
     8281 aagggcatat ttagcagcct ctttttgcca ttcccccatg tcaagacctg tcaagcgaag
     8341 aagaagataa atgaaggcaa ctaatggact aagtaagtgg aaggcttgtc ccatgaggga
     8401 agcaagtgcc atagccatgt tactgaaacc atattcagca ccagcttctg caagaacagg
     8461 caagatacca tagtaaaaac catcattgga gataaagaag gtaccaggtg cagaaactaa
     8521 ggcaataaca agaccccaga aacctgccag ttgttttgga ataatggttg caaaactttg
     8581 tgcaagagcg ctagccatac cagaaccttg aaaaagaccc ataaagatac cagcagcaaa
     8641 gacgagaata accacttgaa ccgcatcacc agcattgtca ccaatgcgtt ttgattgatc
     8701 tttaagtact gggtaattga ccatcaaggc gatacaagtt cccaccgcaa aaagaagaag
     8761 cggtgccata gcaatagatt tgttgaaaga gccagcaact aaccaagtga ttaatccaat
     8821 ggttaggata gcattgaaaa caaagttttt aggacgacga ataaaaaggg tatctgggtc
     8881 agtaatgtct gtgagttgac gcatgtcttc ttcagacaag tcaataacac caagtcttgc
     8941 acgttcttta cgccccatgc ttggagcaac ccaacaaatc acataaagaa gagataaaat
     9001 catacccggt gcaagatagc caagaatttc aggaccgaca ccaagaacag acatcgcacg
     9061 agcagtaggg ccaccccatg gcagtaagtt cataatagta ttttgaagaa taatcaagac
     9121 acctaggttc atgattttca tgtccaattt tttatagata ggtaagaaag cagagcaaca
     9181 aattaaagta gtggttgttc catcaccatt aagagatact gcagcagcaa cgacagctgt
     9241 tgcgataagc accttcattg gatcaccttt agcaaagcga atcatctttt cagtaatcgg
     9301 atcaaacaat cctgcatcta acatgacaga gaagaacaaa atggcaaata gcaacatgat
     9361 acctgtttta gcagtattgt tgataccata cataaccatt ggtccgatag ctgttaggtc
     9421 tttagcaatt ccaccgtcac caacaaaggc aacaaatttg gcatctgcat tgaagtcagc
     9481 tgaaccagtc agtataacag caatcgtcat gattaatgga atcatgacca aagcagtaaa
     9541 aggggtcatt tttttcttca ttaccacgta cataaagaca ataatcatgg cataagccag
     9601 cattgttaat aacatcattg ttatctcctt tttcaagtta tgatttcagt atagatgaaa
     9661 gcgcttataa aataaatact tctttgtgca gaaagacaca aaaaaaacaa tttgaaaatt
     9721 aattttgaaa accattttta ttacatcaga tgacttta
//
LOCUS       DATURV010000050         9446 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DATURV010000050 DATURV010000000
VERSION     DATURV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.050"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            64..4170
                     /gene="cas9"
                     /locus_tag="VCF75_001292"
     CDS             64..4170
                     /gene="cas9"
                     /locus_tag="VCF75_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEP2546850.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASRVDAKAILSARLSK
                     SRRLENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPEFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRSRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSDILKE
                     YPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWKQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVRKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNFGA
                     PAAFIYFDTTIGRNRYKSIKEVLDATLIHQSITGLYETRIDLSQLGGD"
     gene            4170..5039
                     /gene="cas1"
                     /locus_tag="VCF75_001293"
     CDS             4170..5039
                     /gene="cas1"
                     /locus_tag="VCF75_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEP2546851.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNDKEMYLTNIISDYTKKVVKA
                     LNNEGKGVPEFRI"
     gene            5036..5377
                     /gene="cas2"
                     /locus_tag="VCF75_001294"
     CDS             5036..5377
                     /gene="cas2"
                     /locus_tag="VCF75_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEP2546852.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            5367..6029
                     /gene="csn2"
                     /locus_tag="VCF75_001295"
     CDS             5367..6029
                     /gene="csn2"
                     /locus_tag="VCF75_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEP2546853.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYKYEEDSEL
                     KFFDHKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLVVFVNSGAFLTKEEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLITKN
                     MV"
     repeat_region   6132..6431
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6132..6167
                     /rpt_unit_seq="gttttagagctatgctgttttgaatggtcccaaaac"
     gene            6544..7005
                     /locus_tag="VCF75_001296"
                     /pseudo
     CDS             6544..7005
                     /locus_tag="VCF75_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            7075..8907
                     /gene="lepA"
                     /locus_tag="VCF75_001297"
     CDS             7075..8907
                     /gene="lepA"
                     /locus_tag="VCF75_001297"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEP2546854.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDAKK"
     gene            9178..>9446
                     /locus_tag="VCF75_001298"
     CDS             9178..>9446
                     /locus_tag="VCF75_001298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEP2546855.1"
                     /translation="MLTFGGASAVKADNQQLELAKKKNDLMTELHSNLEQMKSGTLPS
                     SNGDVEESTVKKILKYLEERDRLEDQWREKLFEGMQNKLFDGQDGA"
BASE COUNT         3129 a         1500 c         1979 g         2838 t
ORIGIN      
        1 tattagtgaa atcatcaaac tcattatgga tttaatttaa actttttatt ttaggaggca
       61 aaaatggata agaaatactc aataggctta gatatcggca caaatagcgt cggatgggcg
      121 gtgatcactg atgattataa ggttccgtct aaaaagttca aggttctggg aaatacagac
      181 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
      241 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
      301 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
      361 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
      421 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga
      481 aaaaaattgg cagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg
      541 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt
      601 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac
      661 cctattaacg caagtagagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca
      721 agacgattag aaaatctcat tgctcagctc cccggtgaga agagaaatgg cttgtttggg
      781 aatctcattg ctttgtcatt gggattgacc cctaatttta aatcaaattt tgatttggca
      841 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg
      901 gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct
      961 attttacttt cagatatcct aagagtaaat agtgaaataa ctaaggctcc cctatcagct
     1021 tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt
     1081 cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat
     1141 gcaggttata ttgatggagg agctagccaa gaagaatttt ataaatttat caaaccaatt
     1201 ttagaaaaaa tggatggtac tgaggaatta ttggcgaaac taaatcgtga agatttgctg
     1261 cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt gggtgagctg
     1321 catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag
     1381 attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat
     1441 agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa
     1501 gaagttgtcg ataaaggtgc atcagctcaa tcatttattg aacgcatgac aaactttgat
     1561 aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg
     1621 gtttataacg aattgacaaa ggtcaaatat gttactgagg gaatgcgaaa accagaattt
     1681 ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta
     1741 accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa
     1801 atttcaggag ttgaagatag atttaatgct tcattaggca cctaccatga tttgctaaaa
     1861 attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt
     1921 gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat
     1981 gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtagccgtta tactggttgg
     2041 ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata
     2101 ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat
     2161 gatagtttga catttaaaga agatattcaa aaagcacaag tgtctggaca aggcgatagt
     2221 ttacatgaac atattgcaaa tttagctggt agccctgcca ttaaaaaagg tattttacag
     2281 actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc
     2341 gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgtgag
     2401 cgtatgaaac gtattgaaga aggaataaaa gaactaggaa gtgatattct aaaggagtat
     2461 cctgttgaaa acactcaatt acaaaatgaa aagctctatc tctattatct ccaaaatgga
     2521 agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat
     2581 cacattgttc cacaaagttt cattaaagac gattcaatag acaataaggt actaacgcgt
     2641 tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg
     2701 aaaaactatt ggaaacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat
     2761 ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc
     2821 caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg
     2881 aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa
     2941 tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac
     3001 aattaccatc atgcacatga tgcgtatctt aatgccgtcg ttggaactgc tttgattaag
     3061 aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt
     3121 aaaatgattg ctaagtctga gcaggaaata ggcaaagcaa ccgcaaaata tttcttttac
     3181 tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa
     3241 cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat
     3301 tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa
     3361 gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt
     3421 attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta
     3481 gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc
     3541 gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt
     3601 gactttttag aagctaaagg atataaggaa gttagaaaag acttaatcat taaactacct
     3661 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa
     3721 ttgcaaaaag gaaatgagct agctctgcca agcaaatatg tgaatttttt atatttagct
     3781 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg
     3841 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt
     3901 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac
     3961 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaattttgga
     4021 gctcccgctg cttttatata ttttgataca acaattggac gaaaccgtta caaatcaatt
     4081 aaggaagtct tagatgcaac cctcatccat caatccatca ccggtcttta tgaaacacgc
     4141 attgatttga gtcagctagg aggtgactga tggctggttg gcgtactgtt gtggtaaata
     4201 cccactcgaa attatcctat aagaataatc atctgatttt taaggatgcc tataaaacgg
     4261 agctgatcca tttatcagaa attgatattt tgttattaga aacgaccgat attgtcttgt
     4321 ccactatgct ggtaaaacgg ctagtggatg agaatgtcct tgtcatattc tgtgatgata
     4381 aacgattacc aacagctatg ctgatgcctt tttatggtcg tcatgattcg agtctacagc
     4441 ttgggaaaca aatgtcctgg tcagaaacag tcaaatcgca ggtttggacg acgattattg
     4501 ctcaaaagat tttgaatcaa tcttgctatt taggagcatg ctcctatttt gaaaaatccc
     4561 aatctattat ggatttatat catggtttgg aaaattttga tccgagtaat cgagaagggc
     4621 atgcagcgag aatttatttt aatacacttt ttgggaacga tttctcaaga gatttggagc
     4681 atccaatcaa tgcaggtctg gattatggtt atactttatt attgagtatg tttgcgcgtg
     4741 aagtggttgt gtctggatgt atgactcagt ttgggcttaa acacgctaat cagtttaatc
     4801 agttcaattt tgctagcgat attatggaac catttaggcc tttagtggat aagattgttt
     4861 atgaaaatcg aaatcagcct tttcccaaaa taaagagaga gttatttact ttgttttcag
     4921 atacattttc atataatgat aaagagatgt atctcacgaa tattattagc gattatacta
     4981 aaaaagttgt caaagctctg aataatgaag ggaaaggagt tcctgaattt aggatatgag
     5041 ttatagatat atgagaatga tacttatgtt tgatatgccg acggacaccg ctgaggaacg
     5101 aaaagcctat cgaaaatttc ggaaattttt acttagtgaa gggtttatca tgcatcaatt
     5161 ttctatttat agtaagttgc tgttgaataa tacagctaac aatgccatga ttggtcggct
     5221 gagggagcat aatcctaata aaggaaatat tacattacta acggtcacgg aaaaacagtt
     5281 tgcacgaatg atttatttac atggtgaaag aaataattgt attgcaaact ccgatgaaag
     5341 acttgtattt cttggggagg cttttgatga atcttaattt ttccttacta gatgaaccaa
     5401 ttccattaag aggcggtaca attcttgtgc tcgaagatgt ctgtgtattt tcaaaaatag
     5461 tgcaatattg ttacaaatat gaggaagatt ctgaacttaa attttttgat cacaagatga
     5521 aaaccatcaa agaatcagaa atcatgcttg taacagatat tttaggattt gatgttaact
     5581 cctcaaccat tttaaaattg attcatgcag atttagaatc tcaatttaat gagaaacccg
     5641 aagtgaaatc gatgattgac aaattggttg ctacgattac agaactgatt gtctttgaat
     5701 gcttagaaaa tgaattagat ttagagtatg atgaaatcac aatcctggaa ttgattaagt
     5761 ccttaggagt aaaagtagaa acacaaagtg atactatttt tgaaaaatgt ctagaaatac
     5821 ttcaaatttt caaatatctc accaagaaaa agttagtcgt ctttgtcaat agcggagctt
     5881 ttctaacaaa agaggaagtg gctagtttac aagagtatat atcattgaca aatttaacag
     5941 ttctcttttt agaaccacgt gaactatatg attttccgca gtatatttta gatgaagact
     6001 atttcttaat aactaaaaat atggtataat attcttaata aatgcagtaa tacaggggct
     6061 tttcgagatt gaagtctagc tgagacaaat agtgcgatta cgaaattttt tagacaaaaa
     6121 tagtctacga ggttttagag ctatgctgtt ttgaatggtc ccaaaactat cactaactcc
     6181 ctattgcgtg gggaactgtt ttagagctat gctgttttga atggtcccaa aactgtttct
     6241 tgcaatcgca atatttggga tgagttttag agctatgctg ttttgaatgg tcccaaaact
     6301 tagaggccta tttcacagac aaagacatcg ttttagagct atgctgtttt gaatggtccc
     6361 aaaacgactc atcccaacta ccgtaagccc taaaggtttt agagctatgc tgtcttgaat
     6421 ggtctccatt caacattgcc gatgataact tgagaaagag ggttaatacc agcactcgga
     6481 taccttccta ttctttctgt taaagcgttt tcatgttata ataggcaaaa gaacagtaga
     6541 gtgatggaac aaacattttt tatgattaag ccagatgggg ttaagcaagg ggggttagtt
     6601 ggagaggttt tgcggtggat tgaacgccta agacttacgt ttaagtgatt cgagctaaga
     6661 caagctagtt cgaaatactt ggctaagcac gacgaggcct tggtgataaa ccttttgatc
     6721 ctaaacttaa agcttacatg acaagtggtc ctgttttaat taggacactt cttggggact
     6781 aaggtggttt tgtctttttg aaccatgatg agagtaacca atcctacgga tgctctttgc
     6841 ggaaccattc gtgggaattt tgctcaggct cctcgtgatg atggaggtgt ttttaacatg
     6901 gcgtatgggc ctcattcaag agactctgct aggcgtgaga ttttcctttg gtctcatcaa
     6961 agtaatctcg gtagctccgc tattctctca caagataatc cgtaagaaaa gataggcaaa
     7021 ggcctacctt ttttgttata atagaaggaa agactaaatg tataggaaaa tagaatgaac
     7081 agtcaagatt taaaaaaacg tcaggagaag attcgtaatt tctccattat tgcacatatt
     7141 gaccatggca aatctactct cgctgaccgc attttggaaa agacagagac ggtttcgtct
     7201 cgtgaaatgc aggctcagtt attggactcc atggatttgg agcgtgagcg tgggattacc
     7261 atcaagttaa acgccattga gcttaattac acagctaaag atggggaaac ctacattttc
     7321 caccttattg ataccccagg gcatgtggac tttacttatg aagtatcgcg ttcgttggca
     7381 gcctgtgagg gagcgattct agttgtggat gcagcgcagg gcattgaagc acagaccctt
     7441 gccaatgttt acctagccct tgataatgat ttggaaattt taccggttat caataaaatt
     7501 gatttgcctg ctgctgaccc tgagagagtc cgccatgaag tagaagatgt cattggactt
     7561 gatgcttctg aggctgttct ggcgtcagcc aaggctggta tcgggatcga agagattctt
     7621 gagcaaattg ttgagaaggt tcctgctcct acgggtgatg tggatgcgcc tttacaagcc
     7681 ttgatttttg actctgtgta tgatgcttat cgaggcgtta tcctgcaagt tcggattgtg
     7741 aatggtattg tcaaacctgg cgataaaatc cagatgatgt ctaatggaaa aacctttgat
     7801 gttacagaag ttgggatttt cactcctaag gcagttggac gagatttcct tgcaacggga
     7861 gatgttggtt atgttgcggc ttctatcaaa acggtagcag atacccgtgt gggggatacg
     7921 gtgactttag ctaataaccc tgctaaggaa gccttgcatg gctacaagca gatgaacccg
     7981 atggtctttg cagggattta tccgattgaa tccaataaat acaatgactt gcgtgaagcg
     8041 cttgaaaaat tacaactgaa cgatgccagt ttacagtttg aacccgaaac gtcacaagcg
     8101 cttggctttg gttttcgatg cggcttttta ggcttgcttc acatggatgt gattcaagaa
     8161 cgtttagagc gtgaatttaa cattgatttg atcatgacag caccatctgt agtctaccac
     8221 gttcacacaa cagatgagga catgattgaa gtgtctaacc cttcagaatt tccagaccca
     8281 acacgggtgg cttttattga agagccttat gttaaagcgc aaatcatggt gccacaagag
     8341 tttgtagggg ctgttatgga attatcgcag cgcaagcgtg gtgattttgt gaccatggat
     8401 tacattgatg ataaccgtgt caatgtgatc taccaaatcc cgctggctga aattgtcttt
     8461 gatttctttg ataaattgaa atcctcaacg cgaggctatg cgagttttga ttatgacatg
     8521 tcagaatacc gcaggtcaca attggtgaaa atggacattc ttttgaacgg tgataaggtc
     8581 gatgcgctca gctttattgt tcataaagaa tttgcttatg aacgcgggaa aattattgtt
     8641 gagaaattga aaaaaatcat tccacgtcaa cagtttgaag ttccgatcca agccgctatt
     8701 ggtcaaaaaa ttgttgcgcg ttcagacatc aaggcccttc gtaaaaacgt tttggcgaag
     8761 tgttatggtg gtgacgtgtc tcgtaaacgt aaattgctcg aaaaacaaaa ggctggtaaa
     8821 aaacggatga aggctattgg ttctgtcgaa gtcccccaag aagccttctt gagtgtcctt
     8881 tcaatggatg atgatgccaa gaaataacca agcctaaccg cttagccttc agaggagaac
     8941 tcttgtgggt ctacatgacc atcttataaa aaaagaactt tacaatcatt tgcacataat
     9001 gttataattc tattatagtg ttatctcatt attaggtgag cctacattta atcttattaa
     9061 agagaaagag gaaacaaaaa ctgtgaacaa aacaaaacaa aacaaaacaa aacaaaacaa
     9121 aacaaaacaa aacatagcct tctttgtcgc tatggcttaa cctcagcggc tgctcttttg
     9181 ctgacctttg gaggtgcaag tgcggttaag gcggataatc agcaactaga gttagcgaaa
     9241 aaaaagaatg atttaatgac tgaactccat agtaatcttg aacagatgaa aagtggaact
     9301 ttacctagtt ctaatggtga tgtggaggaa agtacggtta aaaaaatact aaaatatctt
     9361 gaagaaagag atcgattaga agaccaatgg agagaaaaat tatttgaggg aatgcaaaat
     9421 aagctttttg atggtcaaga tggtgc
//
LOCUS       DATURV010000051         9299 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DATURV010000051 DATURV010000000
VERSION     DATURV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.051"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(118..633)
                     /locus_tag="VCF75_001299"
     CDS             complement(118..633)
                     /locus_tag="VCF75_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEP2546856.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
     gene            714..1322
                     /gene="recU"
                     /locus_tag="VCF75_001300"
     CDS             714..1322
                     /gene="recU"
                     /locus_tag="VCF75_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEP2546857.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            1309..3474
                     /locus_tag="VCF75_001301"
     CDS             1309..3474
                     /locus_tag="VCF75_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP2546858.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDTSNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(3939..5276)
                     /gene="pepC"
                     /locus_tag="VCF75_001302"
     CDS             complement(3939..5276)
                     /gene="pepC"
                     /locus_tag="VCF75_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEP2546859.1"
                     /translation="MSALTETFTEQLFAHYEASAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQY"
     gene            complement(5461..6285)
                     /gene="nadE"
                     /locus_tag="VCF75_001303"
     CDS             complement(5461..6285)
                     /gene="nadE"
                     /locus_tag="VCF75_001303"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEP2546860.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQAVALQQAGIEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALEVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            complement(6282..7742)
                     /locus_tag="VCF75_001304"
     CDS             complement(6282..7742)
                     /locus_tag="VCF75_001304"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEP2546861.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPENFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAIIGGADATSNVRAGKRFGIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKAYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMALIDNIRKDTHGKSDLK"
     gene            complement(7913..9292)
                     /locus_tag="VCF75_001305"
     CDS             complement(7913..9292)
                     /locus_tag="VCF75_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP2546862.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
BASE COUNT         2968 a         1915 c         1627 g         2789 t
ORIGIN      
        1 tactctccga acggtcaatc tttttaccaa aattatattt cagtattatt attaatgata
       61 cagaaaaata gttcaaaaag caagtattta aataccaata cccctaaaaa tacaatgcta
      121 gctttgtgac atatcattta gccgatcaaa ggttagcaat agcaactgat aatgcggcat
      181 atcttttgct ttcttgagaa aatattttaa gttggtctcg ttttctgtgt cataaaaaac
      241 ataagcccct tctgtctttt ctaataaaaa ctgataatat tgactaaatt gcgtcggttg
      301 ttcgtaattt ggaaagtaat attttacaaa gtcaacagcc ctaaactgac ttaaaacttc
      361 ttgatttttt tcattccacc tatctccatg cgtttcaaac aagaatatag tagctatctg
      421 caagggatac tcctctttta gctcattagc tacttccaaa gcccattgtt caaaacccaa
      481 gttccctgta aagataaacc aatccacacc gttttccaaa taacctatta aatctttacg
      541 aatagcctgt ttaataattg aaactcgtgg gtctttatgg tcaaaaatcc ctatttcaaa
      601 gcttcggtaa cctgtaatta gaatcgcagt cattaggtgt tatcctttca aaaaatatgt
      661 tataataaaa gtgcttaaat ttttagcatt aactagataa tattaggaga acaatggtta
      721 attatcctca taatcttatt cgacaaaaag taagttccgt tcaaaagcaa acaaagcaaa
      781 ataaggtgga cttcgccaat agaggtatgt cctttgaagc tgctatcaac gcgacaaatg
      841 attattattt atctcgtcaa atagctgtca ttcacaaaaa acctactcct gtccaaattg
      901 tcaaagttga ttaccctaag cgcagtcggg caaaaatcgt tgaagcatac tttagacaag
      961 cttcaacgac tgactattgc ggcgtttata aaggacatta tgttgatttt gaagcaaaag
     1021 aaaccaggca aaaaacagct atgcccatga aaaatttcca tttacatcag attgaacata
     1081 tggcttgcgt tttacatcaa aaaggaatct gctttgtcct tcttcacttt tccacactga
     1141 aggagactta ctacctacct gctcaagcct tgatttcatt ttatcagata gataatggca
     1201 gtaaatcgat gcccattgat tatatcagaa aaaatggctt taaggtagca tttggagcct
     1261 ttccacaagt cccttattta aacataattg aacaaaattt tttaggcggt gattacaatt
     1321 aaaaatccaa aaatccttaa gtggctaaag tatgtattaa gtgcaattct tagccttatt
     1381 atccttgtta ttattattgg tggtcttttg tttaccttct acattagcag tgctccgaaa
     1441 ctgtcagaag cccagttaaa atcaacaaac tctagcttgg tttatgacgg taataacaat
     1501 ctgattgctg atttgggttc tgaaaagcgt gaaaatgtaa cagctgatag tatccctatt
     1561 aatctagtta atgctattac ctcaattgaa gataaacgtt tctttaacca tcgtggagta
     1621 gatctttatc gtatttttgg tgctgccttt cataatctaa cgagtcagac cactcaaggg
     1681 gggtcaacgc ttgatcagca actcattaaa ctagcctatt tttctactaa tgaatctgat
     1741 caaaccttaa aacgtaaggc tcaagaagtt tggcttgctc ttcaaatgga gcgaaaatat
     1801 actaaacaag aaatcctgac tttttacatc aacaaagtat atatgggtaa tggcaactat
     1861 ggtatgctga cagccgctaa gtcttattat ggcaaggatc ttaaggattt atcttatgcc
     1921 cagctagccc tattggctgg aatccctcaa gctcctagtc aatatgatcc ttaccttcat
     1981 cctgaagctg ctcaaaatcg ccgtaacgtc gtgttgcaac agatgtacat ggaaaaacat
     2041 ctgacgaaag cagaatatga aactgccatc gcaactcccg tcgctgaagg tctacaatca
     2101 ctccaacagc gctcaactta tccaaaatat atggataatt atctaaaaca agttattgaa
     2161 gaagtcaaaa aagaaacaaa taaagatatt tttaccgctg gtttaaaagt ttatactaat
     2221 attatccccg atgcgcagca gactctttat aatatttatc attctggtga ttatgtttac
     2281 tatccagacc aagatttcca agttgcttca acgattgttg atgtgacaaa tggtcatgtt
     2341 attgctcagc ttggcggacg taatcaagat gaaaatgttt catttgggac taaccaagct
     2401 gttttaactg atcgtgactg gggttctacc atgaagccaa tcacagccta tgctcctgct
     2461 attgaatctg gtgtttatac ttctactgct cagtcgacta atgactcagt ctattattgg
     2521 cctggaacca ctacccaatt gtttaactgg gaccttagat ataacggatg gatgacaatc
     2581 caagctgcta ttatgctatc gcgaaatgtc ccagcagtcc gagcactgga agccgcagga
     2641 cttgactatg ctcgatcttt cttaagcagt ttaggtatta actatcccga aatgcactac
     2701 tcaaacgcta tctcaagtaa taacagtagc tcagataaaa aatatggtgc aagtagtgaa
     2761 aaaatggccg ctgcatacgc tgcttttgca aatggtggta tttatcataa accaaggtat
     2821 gtcaataaag tggaatttag tgatggtaca agtaaaactt ttgatgaaaa aggaaaacgt
     2881 gccatgaaag aaaccacggc ctatatgatg acagatatgt taaaaactgt tctcacttat
     2941 ggtacaggca ccgctgctgc cattcctggt gttgcgcaag ctggtaaaac agggacttct
     3001 aactacactg atgaggaact agctaaaatt ggtgaaaaat acggccttta tccagattat
     3061 gttggtacat tagcgccaga cgaaaacttt gttggcttta ctaagcgcta cgccatggct
     3121 gtttggacag gttacaaaaa ccgcttgacc ccagtctacg gatcaagtct agagattgca
     3181 tctgacgttt atcgtagcat gatgacttac ttaacaaatg gttacagtga agattggacg
     3241 atgccaaatg gtctttatcg cagtggtgga ttcctctact taagcggaac ctatgcgagc
     3301 aacaccgact atactaattc ggtttacaac aatctttaca gcaataacac gacaacagct
     3361 tctagccaaa cgacttcaga tgatactagt agtagcaatg atacaagtaa ttcaaccaat
     3421 acagacaaca atagcaatca tccatctacc gatgataaaa agacaactca ttaatctcta
     3481 actctactta ttgtctgtac ctacctttta ttacatatcc atttacttac ttttaatatc
     3541 taccttaagt gataatctaa ataaaaagcg agtccacgtt ctgtgaggac tcgtttttat
     3601 atgacacaaa tgacctacca catacaatat aaaacaaaaa acagaattct ttaatcgtat
     3661 taaagaaaag gtacaacgtt aagggaatta gcaactgatt tttcaaacgt taacctaaaa
     3721 taaaatagac ttatttaaaa ttgttataaa tagcaactaa tgccataaga gagtatacat
     3781 gaaaaaaaca cgtttccata taccgaagac gtgttttttc atatgtcatt ttcttagcat
     3841 cataattttt ttcatgttca ctttcttatg cggagtccaa tccgtttgct gttcgttttg
     3901 tccagctttt agatttcata cagtcgcaaa gaaaggaatt agtattgtgc taaagcaccc
     3961 attggatccc agggagctag tacttgtggc tctttttcat aagccgctaa ctcgtcagct
     4021 gttaagaatt ctttacggac aacaatttga taggtatatt catccatcca agcatctgaa
     4081 gcaacaaagt aacctttgtc accaaccttc tcaccccatg aattttcaac cttccatttt
     4141 aatggttttc ctgtttcatc taagtcaaca cctgttaaaa ccatagcatg tgtcatcaaa
     4201 ctttcactat aatccaaacg tccagcctta tcttgactaa gattgatatc catactagct
     4261 tcaaaatcgt aagtattagt tgccaaaatg cctttttgac gatctgaaac ttgacccaca
     4321 tcagagccaa accaaacgga ctcaccagcc tgcatctgct tgatagctag ttctttgaaa
     4381 cgtttcatat caaggttgag gtagcgaacc tcacgactac cgaccacatt tcctagcatc
     4441 tcaacagtgt atgatttgcc ataaggctta tcagcagttg gcgcattaat aacagacaca
     4501 taatcagaaa gctttaagcc aacaaatttg tcgtaaaacg cttgcggtgt caaacctttc
     4561 tcaacatgat agtggtcatc tttatcacgg taggcaaaat caaaatgacg aggtggtaaa
     4621 ccaagggtca tggccaagaa gttaaaaatt tcctgaagaa gctctgcttt tcgatcctca
     4681 acttggtctg ccttagcgcc tgacgcaatc aggtcacgaa gaatttgagc atcttggcgc
     4741 aataatttat tcaaatactg gtttaattca cgactgttac ttgatgagat tgattctggg
     4801 taaacagatt ttggcaccac tccatacttc tcaaacaagg aaactaccat gtcccactga
     4861 ccaccatctt gttgtggaac atccaagaga aacttaactt tacgactggt taattcttgg
     4921 tcagctgtag caatgacctg ctccataaac cagttagctt tttcatactt atcccaaaag
     4981 aaagtatgag cttgtgacag ttcaaaattt tctaatttaa actctgtgat taatttgtgt
     5041 cggaaagtgt tcaaggcagc aaacatccag caacgaccag aagccttttg gttagatacc
     5101 ttatctttag ttaaatcaat agaaaacaca aaatcattgt ccacttcact ttgacgggtt
     5161 tctaatgatt ttagcaaacc attatgcgta acggcattct caatagctga aaattttgca
     5221 ctggcttcgt aatgagcaaa tagttgttct gtaaatgtct ctgttaatgc tgacattatt
     5281 gtcctcctaa aatattaaga taagcttcac ctgaaataat attggcaaga tggttattat
     5341 ccagccattc tattctaaag gattacttct attatagacc ttatttgatt ttcttcaaga
     5401 aaaaagttag gataacctaa ctttttttgt tttctttttt tactctttga acatcatctc
     5461 ttatttccaa aaatcatcaa aaatggtgat tggcaggtga cgcttgtgtt gtcctttatg
     5521 ccaccatttt tcaatagttg cttgagctac tttggaaatc agcttccctt ccaaatagtc
     5581 gtcgatgtct tgataggtca cctctagagc aacctcatca gccaagcctg gtttttgatc
     5641 ttctaaatca gcagttggaa ctttttcata caaagcagca tctgctccta aaactttcaa
     5701 aagagccttt ccttgacgtt tgttaagcct aaagagggga agaatatctg ctccaccatc
     5761 accaaattta gtaaagaaac cagtgatgtt ttctgccgca tgatccgttc caataacagc
     5821 accagccatt tggccagcaa tggcatattg acttatcatt cgctgacgtg ctttaatatt
     5881 acctttattg aaatcagaaa tctcgatgcc agcttgttgc aaggctactg cttgaccgtc
     5941 cacagctgcc ttgatattaa tggtaagggt ctgatctggt gcaataaaag ccaaggcctt
     6001 ttgtgcatct gcttcatcag cctgaacccc gtaaggcaag cgaacagcta taaattggta
     6061 agcttgatca cttgtttcct cccgtaactc tgcaatggcc atctgggcta gctttcctgc
     6121 gagggtagaa tcctgacctc cagaaatgcc taagacataa gttttcaaaa atgagtgctt
     6181 acgcagataa gcttttaaaa aatcaactgt tttccgaatt tcttcttgtg ggtcaatgct
     6241 agccttgacg cctaattgtc ggataatctc ttcttgtaag gtcattttaa atcactcttt
     6301 ccatgagtat ctttacgaat gttatcaatt aaggccatct tattttgcca aacatctcgt
     6361 gccaagtcta ctgggtagtc ttgaggattt aagacacgct tgtattcatc ccaaagcttg
     6421 tcaaattctt ttttagcata agccttgatt tcagataagg tcggtagctg gtaaactaat
     6481 tctcccttga caaaaatatc aactaagaga ggaattgcat caaaatttct gacggttttc
     6541 ttaatatagg tataggtcgg atggaacatc tcaatctcag ttaattcatt aacattaata
     6601 tcagtaaacg tgatatagtc gccttctgat ttgccttttt cacgactggt gatgcgccaa
     6661 acttgttttt tacctggtgt ggatactttt tctgcattat ttgataattt gatcgtatca
     6721 cgcatgctac catcttcttg ctcaatagag acaatcttat aaacagcacc taaggcaggc
     6781 tgatcatagg ctgtgatcag tttggtccca acaccccaaa catcaatttt ggccttttgc
     6841 atttttaagt tgaggatggt attttcatcg aggtcattag aagcatagat cttagcctcg
     6901 gtaaaaccag cgtcgtctaa ctgctgacgg actgtttttg agaggtaagc caagtcacca
     6961 gagtccaatc gaacacctag aaaattaatc ttgtcaccca tttctttggc tactcggata
     7021 gctgttggca cacctacttt aagcgtgtca taggtatcaa caagaaacac acaatctttg
     7081 tgggtcttag cataagccat gaaagcatca taatcattac cataggcttg cactaaagca
     7141 tgagcatgcg tacctgaaac agggatacca aagcgtttac ctgctctcac attacttgtt
     7201 gcatctgctc caccaataat cgctgcgcgg gttccccaaa tcgcagcatc tagttcctgt
     7261 gcacgacggg tgccaaattc caaaaggggt tcatcctcaa tcacagaacg aatacgagca
     7321 gcctttgtag caatcaaggt ctggaagtta acaatgttta aaagagctgt ttcgaccaac
     7381 tgacactgac ctagagggcc ttctacttgc acaatcggct cattagcaaa aaccaaatct
     7441 ccttctttag ctgaacggat ggtcaattct aatcgtaact ctttaaggta ggtcaaaaag
     7501 ttttctggat aacccagttc ttcaaggtaa gccaaatctg tttctgaaaa ttgaaactgc
     7561 tctaggtact ccaccatgcg ctgtaaaccg gcaaaaacag cataaccatt gttaaaaggt
     7621 tccttgcgga aatagacttc aaacaccgca tgacgattat gaatgccctg ctcaaaataa
     7681 acttgcatca tattaatctg atacaaatca gtatggagag ttaaactatc atctttatac
     7741 ataaagggga ctcctttgaa ttattgttac tattataaca tattttaaaa aggactcttt
     7801 ggctaggatt tagaaataac ttaagttatc gctaaagtaa aaaaagaact gatccatcat
     7861 gacatcctca aataagaaag agttaatgga ttcagttcta tatcgctaat tattatttaa
     7921 atttgacatt gctataaatc ccaaaaacaa taatccaaat cgttgctcca atggcgccga
     7981 tataggtgga ttcttgtaag aaaagagaga taaagacaaa agcaaagaaa gcaagggtca
     8041 aaggcgttgt cactttatat tttggcatca aataaccatc tgccataaag tccttagatt
     8101 gacggtactt ccagtgagca atcatggtta aggcgtagat agcaatgtat acacctgatg
     8161 aggaggccgt aatgagtgag aacgcatctg caactcctgg taggatatta atcaaagctg
     8221 agataccaac cacaaccgct gaagcaataa tggcacgact tggcacccct tgtcgagata
     8281 aagtattaat ctttaagcga ttggtcaaag cgtttggagt ctcattagca atctgataga
     8341 gatgacgacc ggttgaataa agggttgagt taagcgctga cgctgctgat gtcaagacca
     8401 caaagttaat caaggctgct gcccatttaa taccaatcaa tttgaacacc atcacaaaag
     8461 gagattcatc aactggtaac tgatgccatg gcacaattgc cataatagag accaaggctc
     8521 ccacataaaa gatcacaata cgggttggaa tttcttgaat agcctttggt aaaacttttc
     8581 ttgggttagc cgtctcagaa gtggtaatcc ccacaaattc gatagcttga taagcaaaga
     8641 aaaccatttg gaaggccata aagaacttta gtttcccatt tgggaacatg gaaaaatggt
     8701 caaaaatatt ggaaaggcta gcatggcctg tgtgagtttc aaaacctgtc aataccatga
     8761 aaatagctgt tgcaatcaaa gctaaaatag ctaaaatctt aatcatggca aaccaaaact
     8821 ctgtttcccc aaaaacgcgc acagcaatta agttgatcga actaagtaag actaagaaaa
     8881 ccaactgaat caaccaggct ggccagcttg ggaaccaaaa ttgcacataa gcacctactg
     8941 ctgtgatttc tgccattcca ataaaaataa gggaaatcca ataggataga cccgaaaaat
     9001 aaccccagcc tggaccaatg tatttggaaa taaaattgat aaaggtgtgt tgatctggat
     9061 cataatagag catttcaccg atggcacgca tcatcataaa catgaaggca cctgtaatca
     9121 tatacacaaa aataatagat ggtcctgtta aggcaataga gcgtcctgcc cctaaaaaga
     9181 gacctgttcc aatcgttcct gcaatcgcaa ttaactgcac atgacgattt tctaagcccc
     9241 ttaccattcc attttctagt tcgttattgt ctgtttgttc ttttatagac attctaaac
//
LOCUS       DATURV010000052         9035 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DATURV010000052 DATURV010000000
VERSION     DATURV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.052"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            108..2525
                     /gene="hylA"
                     /locus_tag="VCF75_001306"
     CDS             108..2525
                     /gene="hylA"
                     /locus_tag="VCF75_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEP2546863.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQTPTTTDSEKKVVQSQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASANITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTTPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTFVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYCSYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATTFSNHSLSHQGFYSFPH
                     PVKQNQQ"
     gene            complement(3200..4963)
                     /gene="lpdA"
                     /locus_tag="VCF75_001307"
     CDS             complement(3200..4963)
                     /gene="lpdA"
                     /locus_tag="VCF75_001307"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEP2546864.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAM
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(5290..6699)
                     /locus_tag="VCF75_001308"
     CDS             complement(5290..6699)
                     /locus_tag="VCF75_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEP2546865.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPTVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKVAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLACKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            complement(6884..7882)
                     /locus_tag="VCF75_001309"
     CDS             complement(6884..7882)
                     /locus_tag="VCF75_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEP2546866.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            complement(7941..8909)
                     /locus_tag="VCF75_001310"
     CDS             complement(7941..8909)
                     /locus_tag="VCF75_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEP2546867.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
BASE COUNT         2707 a         1912 c         1618 g         2798 t
ORIGIN      
        1 gccattattt atgtaagggt ttataaaaat actttaacgt ggtatatttt tacttagtaa
       61 accaatctac ttttattatc tcatttttat attggaggaa tttatcagtg aatacttatt
      121 tttgcacaca ccataaacaa ttactacttt attcaaacct attccttagc tttgctatga
      181 tgggccaagg aactgccatt tatgccgata cactgacttc aaattcagaa cctaataata
      241 cttactttca aacgcaaacg cccactacta cagatagcga aaaaaaagta gtacagtcac
      301 aacaaaaaga ctactatact gaattgttag accaatggaa cagtattatc gcaggcaacg
      361 atgcttatga taaaaccaat cctgacatgg tcacttttca taataaagct gaaaaggatg
      421 ctcaaaacat tattaaaagc tatcaagggc ctgaccacga aaatagaact tacctttggg
      481 aacatgcaaa ggattattcc gcttctgcta atatcacgaa aacttaccgc aatattgaaa
      541 aaatagcaaa acagatcact aatcctgaat catgctatta tcaagatagt aaagctattg
      601 ctattgtaaa agacggtatg gccttcatgt atgaacacgc ttataatcta gatcgtgaaa
      661 atcatcaaac aactggaaaa gaaaacaaag aaaattggtg ggtttatgaa attggaactc
      721 ctcgtgctat taataatacc ttatccttga tgtatcctta ttttactcaa gaagaaattc
      781 ttaaatacac aactccaatc gaaaaatttg tgcctgaccc tactcgtttt agggttcgcg
      841 ctgccaattt ttcacctttt gaagccaata gcggaaattt aattgatatg ggacgtgtta
      901 aactcatttc cggtattctt cgtaaagatg atctcgaaat tagtgataca atcaaagcaa
      961 ttgagaaagt tttcacgcta gttgacgaag gaaatggttt ttaccaagac ggttctttaa
     1021 ttgatcacgt agttactaac gctcaaagtc cactttataa aaaaggcatt gcttacactg
     1081 gagcttacgg taatgtgctt atagatggct tatcgcaatt aattcctatt attcaaaaaa
     1141 caaagtctcc tatagaagcg gataaaatgg ctactatcta tcattggatt aaccattctt
     1201 ttttccctat catcgttcgt ggagaaatga tggatatgac tcgagggcgt tctatcagtc
     1261 gttttaatgc tcaatctcat gttgctggca ttgaagcact tcgtgctatt ttacgtattg
     1321 ctgacatgtc tgaagagcct caccgtttgg cacttaaaac acgtataaaa acactcgtca
     1381 cacaagggaa tgctttttac aatgtctatg ataatttgaa aacctatcac gatatcaaac
     1441 ttatgaaaga actactaagt gatacttttg ttccagtcca aaaacttgat agttacgtag
     1501 ctagtttcaa tagtatggat aaattggcac tatataataa taaacacgat tttgcttttg
     1561 gcctatcaat gttttcgaat cgaactcaaa attatgaagc tatgaataat gaaaatcttc
     1621 atggctggtt tacttctgat ggaatgtttt acctatacaa taacgattta ggacactaca
     1681 gtgaaaacta ttgggcaacg gtaaatccct accgcttacc tggaaccaca gaaactgagc
     1741 aaaaaccact agagggaact cctgagaata ttaaaacgaa ctatcaacaa gttggcatga
     1801 ctagtctctc tgatgacgct tttgttgcaa gtaaaaaact taataacaca agtgctctag
     1861 ctgctatgac cttcactaat tggaataaaa gcctcactct caataaaggg tggtttatct
     1921 taggaaacaa aataatcttt gttggtagca atatcaaaaa ccaatcatct cacaaggcgt
     1981 atacaactat tgaacaacga aaagaaaatc aaaagcaccc ttactgttct tatgttaaca
     2041 atcaacccgt tgacttgaat aatcagctag ttgattttac aaacactaaa agtattttcc
     2101 ttgaaagtga tgatcccgct caaaatattg gttactactt cttcaagcca acaacactta
     2161 gcataagtaa ggcacttcaa acagggaaat ggcaaaacat aaaagctgat gacaaatcac
     2221 cagaagccat caaagaagtt tcaaatacct ttatcactat catgcaaaac catactcaag
     2281 atggcgatcg ttatgcttat atgatgcttc caaatatgac tcgtcaagaa tttgaaacct
     2341 atattagcaa gcttgatatc gacttgttag aaaacaatga caaactggcc gctgtctacg
     2401 atcatgatag tcaacaaatg cacgtcattc actatgaaaa aaaagcaacg acgttttcaa
     2461 atcatagtct ttctcatcaa ggcttttata gttttcctca tcctgtcaag caaaatcagc
     2521 aataaaagct tgctcatcaa ggaattgcag ctaaaaataa tactcttaac tctcataaaa
     2581 ttccccacaa aagacaacga cgcttaccgc atactgggta tcagtcctcc tcattagaat
     2641 tcttaggtgg agctttagtt gcttctttta accatatcac aaaacccttt cgtaagaaag
     2701 acttaaggat ttagctgtta ttggaaagta agggttaaca atgaaacgtt attaaagaca
     2761 aaaaaactgg aaactgcact gagccccaaa agttggctac tctatttgaa gtaaaggatt
     2821 tagttctgtg ttgtagagga ctaagtcctt ttcgttttat tttgatacgg ttattggtgc
     2881 aataatcgat gataataata ggtagaacga gtcaatttag ccgtccttaa aagaaaatct
     2941 aatcagaact cgtctttatc cattctttat aatggttttt tgtcttcttc ttttcagaga
     3001 tgcttctttc aaacggagtt ctctcggttt ttttagcgct aaatgtctag cttatcttca
     3061 tagcttaatg tcatacaaaa acaccccaaa cgttagggtt ttatatctaa cgtttggggt
     3121 gcagttcata gttgtgctag tcttttttgt gatctgttat gtgatgatat ttttcattag
     3181 ttaataagac aaagacgttt tacttacgtt ttggtgggtt atggatagct tcgccaagca
     3241 catctgcaaa ggcttcgtac atcacttcag agaaagttgg gtgtccatga attgataata
     3301 acaattcatc aacggttaat tcagattcca taatagtagc tgcttcgtta atcatctccg
     3361 ctgcagctgg accaataata tgaacaccta agatttcgtg gtatttggca tcagcaataa
     3421 ctttgacaaa accatgggct tcatttgaag caatcgcgcg tccgttaccg gtaaagctat
     3481 tttttccgat aagcacatcg ccatattgct cacgcgcttg ttcttcagtt aaaccaacca
     3541 ttgccacttc agggtgtgtg taaacagccg ctggtgtgta tttaaggtta gctttacgag
     3601 ttgtgttgcc atgcatagca ttttctgcag ccacttcacc catacggtaa gcagcatgag
     3661 caagcatttt cgttccgtta acgtcacctg gtgcatagat acctggaatt gatgtctctt
     3721 ggtagtcatt aactttgata cggttacgat ccatttcaag gttaaggttt tccagaccgt
     3781 tcatttgtgg tacacgaccg atagaaagaa gagctttttc agcgacaact tcttcaccat
     3841 tgttaagttt caaggtcaat tggttatttg cttcaacaat ttcagagaca ccaacagatg
     3901 ttttgatctt catgcctttc ttagaaagga ttttttgaag ttcaagagag acttccttat
     3961 ccatagctgg gataatgcgg tcagccattt caataacggt aacatcaaca ccgtaagatg
     4021 cccaaacaag tccaagctcg ataccaacaa caccaccgcc cataactgct agtgattttg
     4081 gcatttcacg aaggtcaagg atatcgtcag acgttaagac aagtttagag tcaattcctg
     4141 ggatattgat acgtgatact ttagaacctg tagcaaggat aacgttgcga cccttaatgg
     4201 tttgtgaccc aatggttaca gtcttatcag ggttgacttg accaagccca ttaaagatag
     4261 tgactttatt agcttttaga agaccttgaa caccacctgt taaggtttta acaacggtgt
     4321 ttttaaagtc aactgttttg tccatgtcaa tggtatagtt agttgaggca aggttaatcc
     4381 cacgtcctgc tgcaatctta ataccatcaa ggatttcagc atttttaagg taagttttgg
     4441 ttgggataca ccctacgttc aagcaagttc caccaaattc agatttctca acgatggcaa
     4501 ttttaccacc aagttgagca ccacgaatag ctgcataata acctgcaggg ccaccaccaa
     4561 caacgatgat gtcatactca tcatcagcaa gtgcagcttt tggtgcagct gaagcagctt
     4621 gagctggtgc ttttggcact tcaagtccag cagcttctaa atcttctgtt gtacgagcaa
     4681 cattcacatc tgaagcagct gggctagaaa cctcaacaga ttcaccttca gcaccgatat
     4741 atccgataac ttctgttaca ggtactgttt cacctgcttg acgtgtaatt ttaagaagaa
     4801 cacctgagtc ttctgcctca agttccatat tggttttatc tgacatgatt tcaagaagaa
     4861 tatcgccttc attgacagta tcaccttctt gttttttcca ctcgatgatt tcaccttctt
     4921 gcatgtcaac accgagtttt ggcataataa tttcaacagc cataatttaa gataccaaga
     4981 gcaaggagaa agcgattgaa tttttgcggt aagtcagaaa tctttgattt cgttgacgca
     5041 cccacgtcag gacgactagg actttccgaa acaaaaattg aggaaagttc agacgtctac
     5101 gacgctaaga ttagccaact cttaccattt ttatccgagc ttttcgctcg gattcaattg
     5161 cttatcactc atttgcatga gagctcctcg ttctacatcc tttctatatt tttgagaaaa
     5221 gggcaccaag tctaaactta gcaaaatccc ttttattatt gaatgctttt cttgtttaaa
     5281 actgattgat cagattaaca attcaaatgg attttccatc aatttcttga gatcaaccat
     5341 gaattttgca ccgttcatac catcaactaa acggtggtcg atggttaggc acatcgccat
     5401 aattggacgg gcaacaatct caccatccac aaccgttgga gttgggatag ttgctccaac
     5461 accaagaatt gctgagtttg gttggttaat aattgggtta aatgtcttcg taccaaacat
     5521 tcccaagttt gtgatagaga aggttgatcc agacatttca gctgccttca acttaccagt
     5581 ttgcgctttt ttaatcacat cttttgaagc aagcacaaaa tctgacaagc tcatcttgtc
     5641 agccccatgg ataacaggaa cgataagacc atcatcaaga cctacggcaa taccaaggtt
     5701 cacaaagcgg tgcaattcaa tatcattagc atcattgatg agggaagcat tcatgtactc
     5761 atgctcaggt ttcatcaagg ttttaaccac tgccatacca atcaagtctg tgaagctgac
     5821 tttaaggcct gttttagcca tgattgggtc aattaacttc ttacgaaggg caatcatctc
     5881 tgtcatgtca atatcgtagt taagcgtaaa ggtaggagca gtaaggtaag agtttgtcat
     5941 acctttagag attgccttac gcatagctga cattggcttg tgttccacac cttctggaag
     6001 atcaacaact ttttcttctt tagctgctgg ggcttttgct tcagctggtt tagccgcttc
     6061 aagaatagcc atgatatctt ctttcatgac cttaccgtta aagccagtac ctgatacggt
     6121 tgccaaatca atacctttgt ctgctgcaat tttacaagca agtggtgaag ctttcggttg
     6181 agcaccttta aagttttcaa catcttcttt atggacacgt ccttttggtc ctgttcctgg
     6241 aacttggcca aggtcaatgc ccatttctgc tgctacctta cgtgcagctg gagtagcacg
     6301 aacttttccg ccatttcctt gcggaatagc tgtcgctgcc acttgaggag ctggacttgc
     6361 cacattttct tttggagcaa ctgttggtcc agcatctgct gaagcaggaa ctggaatttc
     6421 tgttgttttt tcacttgaag caatagtatc tactgattca ccctctgcac caatgtaacc
     6481 gataacttca gttacaggta cagtatcacc agctggacgg acaattttca atagcacacc
     6541 tgagtcttct gcctcaagtt ccatattggt tttgtctgac atgatttcaa gaaggatatc
     6601 accttcattg acggtatcac cttcttgttt tttccactcg atgatctcgc cttcttgcat
     6661 gtcaacgccc agctttggca taataatttc aaatgccatt tctacatcct ttcacgattt
     6721 gataagcgcc atcaacatcc ccaccttgct acgaaaacat acgaaaggtg aggtgccgcg
     6781 acacgggata agacaactca gttcttctaa aaactttcaa aatagttttt agctgagacg
     6841 ttactgataa acatagcctt tatcggacca cttgccttat cttctagttg cctttgtttg
     6901 ccattttcac aatagcagct ttgattttct caacgtctgg taagatagct tgttcaagta
     6961 cacgtgcata aggtactgga acatcttcac ttgcaagacg aacgattgga tgatcaaggt
     7021 aatcaaaggc ttcactttcg gtaatcatgg ttgcaatttc cccaatgaat ccaccggtct
     7081 tgtaggcatc gttgacaagc atcaatttac ctgttttctt aacagattca ataatcaact
     7141 ctttatcaag tgggatgagg gtacgtgggt caacaacttc aacgttaata ccatccgctg
     7201 ctacttcttc agccgcttga aggacacgct ccaacatacg accatatgac acaattgtca
     7261 aatcagttcc ttcacgtttg atgtcacctt tgccaagtgg gatatagaag tcaggatctt
     7321 ggttaacttc ttctttttta ccataaagcg cttttggttc catgaaaaga acaatgttat
     7381 tatcacggat agctgatttt aataagcctt tagcatcgtt ggcattacca ggagcaacaa
     7441 ctttgattcc tggaatgtga gtcaaccatg cttcaagaga ttgtgagtgt tgcgcagcgg
     7501 aaccaatacc agaacctgat gccacacgga aggttacagg ggtaataagg cctccaccga
     7561 acatgtaatt atttttagca ccattattca cgatagcgtc catcatgatg gtaaggaaat
     7621 ccatgaaggt aacgtcgaca attggacgta gtcctgtaat cgctgcacca atagccgcac
     7681 cagagatagc agcttctgaa attggggtat ctttaacacg tttaggacca aattcttcaa
     7741 tcattccaac agaagtcccg aagtctcctc cgtaaacccc tacgtcttct cccataagga
     7801 aaatgttttc gtccttgcgc atttcctcag tcatagcaag gttgactgct tcacgcaaag
     7861 ccattaattt tgtttctgac atcattttta cttctccatt tattattcaa atacgattgc
     7921 ttcttactga caaggtgatc ttagtcaacc caaacgtctt cgaaggctac tgaaagttct
     7981 ggatctggac tgttttgtgc aaattcataa gcatcgtcaa cttctttttt gacttgtgct
     8041 tgaatagcat ccaattcgtc atcagtggca atgccttcgc ttgtcagata agtacgatat
     8101 ttgatcattg gatctttttc tttccactca tccacttctt ctttagtacg gtatttacca
     8161 gcatctgcag tcgagtgacc aaaccaacgg tatgattcta cctcaacaat agctggacca
     8221 tttccaccac gaacatgttc aacggctttg cccattgttt catagacagc catcacgtca
     8281 ttgccatctt cacagtagaa accaggaatt ccgtaagctt ctgcacgtgt gtataaatgt
     8341 ggtgtatttg ttgcattatt gatgctcatt gaaattccat aacggttgtt gatgatgaag
     8401 aaaataacag gaagtttcca tgttgcagcc atattaacag actcgtggaa tgatccttcg
     8461 ttagtagcgc catcacctga gaaagcaaca gctatattgt tagttccttt gtattgttga
     8521 gtgagggcag caccaactgc aagagcgtaa ccaccaccaa cgataccatt ggtaccatag
     8581 tttccttttt caaaatcagc caagtgcatt gagccaccac gccctttaga aacaccagtt
     8641 gctttaccag caagctcagc catcatttta ttaaggtcca tgtctttagc gattgattgt
     8701 ccatgtccac ggtggtttga aaaaataatg tcatcgtaag acaagtgagc caccgctcca
     8761 acattggctg cttcttcacc aactgagaag tgagtcatcc cttgtacaaa tccacgacgg
     8821 acgagtttat taatacgtga atcaaattca cgaatacgtt ccattttaag gaacatatct
     8881 aaatgttgtt ctttagaaac tgttaccatt tcggcctcca tattatcata ttactttttt
     8941 atgataaaac tttttttcac aatttgcaaa taattaaatc gctttctacc ttaccttatt
     9001 tggtttttta gaaatgcaat agtttgtgaa attac
//
LOCUS       DATURV010000053         8798 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DATURV010000053 DATURV010000000
VERSION     DATURV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.053"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            58..540
                     /locus_tag="VCF75_001311"
     CDS             58..540
                     /locus_tag="VCF75_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEP2546868.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            629..1681
                     /gene="citC"
                     /locus_tag="VCF75_001312"
     CDS             629..1681
                     /gene="citC"
                     /locus_tag="VCF75_001312"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEP2546869.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYTLGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAVVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDRSLIPFSVRKRLIQEGLAHLDNVIYHETGPYLISQ
                     ATFPAYFQKEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWELLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(1791..3185)
                     /locus_tag="VCF75_001313"
     CDS             complement(1791..3185)
                     /locus_tag="VCF75_001313"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEP2546870.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFI
                     QKSIENGIDIVRIFDALNDSRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEDIKEKII
                     GDEAVITCRPADLIEPQMIYLRDEIAPYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(3200..3778)
                     /gene="citX"
                     /locus_tag="VCF75_001314"
     CDS             complement(3200..3778)
                     /gene="citX"
                     /locus_tag="VCF75_001314"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEP2546871.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEAFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            complement(3771..5303)
                     /gene="citF"
                     /locus_tag="VCF75_001315"
     CDS             complement(3771..5303)
                     /gene="citF"
                     /locus_tag="VCF75_001315"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEP2546872.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSIREAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPI
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHP
                     SAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINP
                     NRQDLADHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            complement(5306..6193)
                     /gene="citE"
                     /locus_tag="VCF75_001316"
     CDS             complement(5306..6193)
                     /gene="citE"
                     /locus_tag="VCF75_001316"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEP2546873.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSVMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIV
                     DVEAVIERVERENGIEVGRTRMMAAIESAEGVLNARDISKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTKKEIDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            complement(6181..6489)
                     /gene="citD"
                     /locus_tag="VCF75_001317"
     CDS             complement(6181..6489)
                     /gene="citD"
                     /locus_tag="VCF75_001317"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEP2546874.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEQTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGIDQYDWKEIDSWNV
                     "
     gene            complement(6677..7798)
                     /locus_tag="VCF75_001318"
     CDS             complement(6677..7798)
                     /locus_tag="VCF75_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEP2546875.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIETELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            complement(7843..8238)
                     /locus_tag="VCF75_001319"
     CDS             complement(7843..8238)
                     /locus_tag="VCF75_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEP2546876.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVGEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            complement(8295..8618)
                     /locus_tag="VCF75_001320"
     CDS             complement(8295..8618)
                     /locus_tag="VCF75_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546877.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSAIAAGDRPNSQFKVKRILKRNSEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
BASE COUNT         2580 a         1816 c         1779 g         2623 t
ORIGIN      
        1 cataccattt ttagacattt tgttataata aaaagaaacg acttgaggag ataaaagatg
       61 ccaaagagaa taagaaaagc tctaggagta gttgggaaac tgatgtcaat tgttcctgac
      121 actactgaaa ttattggaaa aacgattgat aacagtcgac caattatcga aaaacgtatg
      181 gaacaaaaac atgaaaaaga gatgcaacta cgcacgatag atgatgtgat taatcttcct
      241 gttgatcagg cacaagctca tttggaacaa cttggctttg tagtagccac tatacctgct
      301 aagcctcata aaaaatggct tcatagtaat ctcaatgaag tggttgctat gtctccaaaa
      361 tctggtaaat ataagatagg aagcctgatc aaactctact acattactgt tgatgttttg
      421 gaaaaaagtc aagacttatt agaccaagaa aacttgcgga ctgtggaacg caatcaaaaa
      481 atcgctgata cttttgagtc tgtcaaacac attcgatttc cttttaaaaa atggcgttga
      541 taaaaaacag agttgccttt agatagatca gcctgttttg gtactaagtt atgttatgat
      601 tcaataaaat taaagataag gagaagaaat gccttactac actatttcaa aagtctttcc
      661 ttcagataaa acgacaatgg ctagtgtaaa gaacctactt catcaagaag gtattcgttt
      721 ggatgctcat ttagattata cttgtgccat catgaatgcg caaaatgatg tgattgctac
      781 cggttcttac tttggaaata gcttgcgctg tctttgcgta tccagtgcct atcaaggtga
      841 aggacttctt aataggattg tgagtcatct cattgatgaa gaatataccc ttggcaacta
      901 tcatcttttt gtttatacga agacctcttc tgctgctttt ttcaaagatc ttggttttac
      961 tgaaattgtc catatcgaca atcacatcag tttcttggaa aataaaaaaa ctggttttca
     1021 agattatctg atgacactta ataagcctga gcaaacacct ggtaaagtcg ctgctgtcgt
     1081 tatcaacgct aaccccttta ccttagggca tcaattttta gtagagaaag ctgcaagaga
     1141 aaatgattgg gttcatctgt ttatggtcag tgaagaccgc agtctaattc ctttttcggt
     1201 gagaaagagg ttgattcaag aaggtctagc tcatcttgat aatgtcatat atcatgaaac
     1261 aggtccttat ttgattagcc aagcgacttt cccagcctat ttccaaaaag aagacaatga
     1321 cgtgattaag agccaagctt tgctggatac cgctattttt ctaaagattg ctcaaacctt
     1381 acagattaca aaaagatacg ttggagaaga gccaaccagc cgagtaactg ctatttacaa
     1441 tgaaattatg gcagagcagt tgcagcaagc tggtatcctc ctagatattt tgccaaggaa
     1501 agctatcaat cagcagcaag atcctatcag tgcctcaacg gctaggcagg cattgaaaga
     1561 taatgattgg gaacttctgg caaaactcct tcccaaaacg tctttagatt atttttgttc
     1621 gctagaagcc caacccatta ttaaaaagat acaagccact tcatctgtca aacattacta
     1681 agataaaaaa cctatctgat tatcaaaatc aggtaggttt ttggtattgt tcttacagca
     1741 aatgcgtttc accaaggaga ttaatggtta agaggattag tcattccctg ttagtcttga
     1801 atgtccagtt gtacagtaac ttcttgaacc ggaacatcat agaaaggatc ttcacggcgt
     1861 cccaagaaat ctctggcttg ctgtgggaaa ctcgcatagc ttaacacgtc ttcttctgaa
     1921 cgagcgtatg gagcaatctc gtcacgtaga taaatcattt gaggctcgat taggtcggct
     1981 ggtctgcaag taataactgc ttcgtcacca atgattttct ctttgatatc ctctgctaat
     2041 ggggctggtg attgaccgta tagccctcga acatagtctt tgatttcatt tggaactacc
     2101 ttgtagcgtt cccctgagat gatattcatc aaggcctgtg tgccaaccat ttgtgataat
     2161 ggcgttacaa gtggtggata tccaagatcg gcccttactt taggtacttc tgctaagact
     2221 tcttcgtatt tatcagctag gccttgctcg gttaattggc tcaataggtt tgatagcata
     2281 ccacctggaa cttggtaaat caatgttttt ggctcagtgt ctttgacttt aggatttaaa
     2341 atccctattt ctcgatagtg gtcacgaata gtgttgaaat attctgcaac tttggccacc
     2401 tcttgcatat ccaagccagt gtcgaagccc aaatccgtca aggcaatcgc cattgactct
     2461 gtggcaggct gactggttcc ccctgaaaaa gaggaaatcg ccgtatcaat aatatcagct
     2521 cctgcttctg ctacttttaa gtaagtcatt tctgaaatac cactggtagc atgggtatga
     2581 acctcaagag ggatagttgt attctccttg atacatttga ccaattggta acctatttca
     2641 ggagttaaga caccagccat gtctttgata caaatggagt cagctccgat agcttggtaa
     2701 gctttcgcca attcaacaaa gtagtcaatg gtatgtaccg gacttgtcgt gtaagaaatg
     2761 gcaacttgag catgccctcc aaatttttta gtcgctgaaa cagctgtttg caagttgcgt
     2821 gagtcgttta aagcatcgaa gatacggaca atatcaatcc cgttttcaat ggatttttga
     2881 ataaaggatc tgaccacatc gtcagcataa ttgcgatatc caagaaggtt ttgtccgcgt
     2941 aagagcatct gaagtttagt ctttttcact gctttacgga tggctcttag ccgttcccaa
     3001 gggtcttcat ttaaaaagcg taggcaggaa tcaaaggttg ctcctcccca catttcaagg
     3061 gcatgataac cagcattgtc tagagtttct agaataggaa tcatttcctt ggttgtcata
     3121 cgtgtggcaa tctgactttg ttgaccatca cgtaaaactg tttccgttat acggattttc
     3181 gtcataattg ttctcctcat caacctgtct tatctgatga cgatgattgg ttgtctttat
     3241 tgaaaaatga atggagtaat tttgaaatag cagtctgcat ctcctcgaca ctgtgcttac
     3301 gtaggcgacc gcagactttg gcctctttag aacagataaa acattgccta ggggagcctc
     3361 ctaatacggt tctgctaatt gaatgaggca gatcattttg caagaccaag acatctaagt
     3421 ccatgagacg acctattggc aactctgtct ctaaggctat catttttaac ttcaggtcgc
     3481 ggctaggtag acttgtgatg aggtaatact cataacccgt tgtaggcagt aatcgcaact
     3541 ggtaacaaat cttatcgtca gctaaggcag tttgaatggc cttaatcact atatcaaaag
     3601 cttcaagaag tttaggagag gttttaattg gtccagggat attcatggtg accgataata
     3661 agctaccttc tgggtactcc tttaataaat gcagctgacg cagagctctt tcttcgcggg
     3721 ctcttaacat atcactaagt tggatagctt cgcctgaaaa ataagtgtct ttacacattg
     3781 taaaccacat ccatgaggct accatcacgg tattcaataa gagcaacaac cttatcacca
     3841 tattgaatac gctcaggtgt gccaacgata gcgtaagcct tctcttttag ctcctcaata
     3901 ctaaattgtg gcacattgag ggatttgaaa tggtctgcta agtcttgacg atttgggtta
     3961 atggcaattc ccacttctgt gacgaccaca tcgacacttg ttcctggtgt aatgaccgta
     4021 ttaacctcat ctacaaaagt tgggatgcgt ccgcggataa gtggtgaaat aaccaaactc
     4081 atcttagccg caaaggcagt gtcgcagtgt cctccagaag cgccacgaat cacgccgtca
     4141 gatcctgtca tcacattaac attgaagtta gtatcaactt caagggctga taaaatacaa
     4201 gtgtctaatt gattgataac agcacccttg ctcaaaggtg aggcatacat gttggcgtca
     4261 atttcataat gttcagcatg cttaccaaga gaaacagctg atgggtgatc aaagtcttgg
     4321 acatcaagaa ttttttcaac cagtccttct tcgagcaact ccaccatagc attggtaata
     4381 ccaccaagag caaagctagc tttgatattt tctttaatca tagcttcacg catgaaacgg
     4441 gttactgcca aagaagcgcc acctgttcca gtttggaaag agaatccttc tttgaaataa
     4501 ggagaattag tgatgacctt agccgcgtat tctgcaatta agagttcttt aggatttttc
     4561 gtaaaacgag ttgccccttt ggcgattcct tgaggatctc cgatagcatc cactgttacc
     4621 acatagtcaa catctgtttg aggaatgctg attggggtat taggataagg aaccaaatta
     4681 tctgtcaaga taaccacctg atctgcatat ttggcatcaa tcatggcata gcccaaagaa
     4741 ccacaggttg ctttcccttt tgtcccatta acatttccgt aagcatctga acttggggcg
     4801 cccagaaagg caacatcaat atggatatcc ccactagcaa tggcacgagc gcgaccacca
     4861 tgggagcgaa taaccacagg attttccatt agtccttctg aaatggctgc ccctactttg
     4921 tcacgaagac cagatgaagt gatgttggta accacaccat ttttgatgtg atcaatcaaa
     4981 ggctcatgta cattagcgat ggaacttgga gcaatagata ggtttttaat gcccattttg
     5041 gcaatttcct ccaagaccat attcataatg aaatcccctt cacggaaatg gtggtggaat
     5101 gaaatggtca taccatctgt taagccagtt ttttcaatcg cttcacggat agagcctaat
     5161 aatttagaat ctccaggttt aactggttta atacggcgac ttgattcgtc atactgtttg
     5221 atatttgcga gttctccttc aaagacacca tactggtcgg cgtatggttg aggaatgtcg
     5281 cgaccgagtt tatttgttac catctttaaa tgtcctcctc agataaaaca cctgctgctg
     5341 ttgctagggc aatgacacgt tctgcacgtt caacgattgg tttatcaacc atttttccat
     5401 ttaaggaaat aacgccagag cctttgcttt cagcttcacg aattgcccag atgacttgtt
     5461 tggcatggtc aatttctttt tttgttgggg tataaatctc attgaccaga ggaatttgac
     5521 gagggttgat tactgattta ccgtcaaacc ctaactgttt gatcatgcga acttcgtttt
     5581 ggaacccttc ggtattattg acatcagaat aaacagtatc aatggcagca attccagcag
     5641 cacgagcagc gtgtaaaatc atgctacgag caaagaataa ttcttgacca tctgggtaac
     5701 gacgcgtttt catattcgtg acatagtctt ctgctccaag agcaatacca atcaagcgct
     5761 tagaagcttt ggaaatgtca cgagcattta gaacaccttc ggctgactca atagctgcca
     5821 tcatgcgtgt gcggccaact tcaatgccgt tttcgcgttc gacacgttca ataacagctt
     5881 ccacatcaac aatatcttgg gcagtctctg tttttggaag acgaatcacg ttgacacctg
     5941 ccaaaacaac agcttcgatg tctaaagccc cacaagaatc aagaccattc acacgaacaa
     6001 ccgtttcaac gcttgaataa tcaaaagttt taagcgcaaa atggacgaga gctctagagg
     6061 tgtctttttc tttgagtgaa acagaatctt ccaagtcaaa catgacggaa tcagcgccaa
     6121 ataaaggagc atctcgaagc atggctgcat tggcaccagg cacaaacatc attgttcttc
     6181 ttaaacgttc catgagtcaa tctccttcca atcatattgg tcaataccag cagcgcgatg
     6241 aacagctgcg atggttctcg cttgaatggt gcaatctaaa gcacctttat caacagcctc
     6301 tactttggca gccgtcacct tcaaattcac tagggtttga tgaatgagtt gacgaatgtg
     6361 attgccaaat tgtttttcaa cactactgtc tagggttatt gtaatggttt gctcgtcatt
     6421 aggggatact gtaatcatta ggtcacttga ttcaagtgac ccagcaacgg cagtttgttt
     6481 aatatccatt gcgtttacct ccttgatttt cttaaatgat attattattt actaactggg
     6541 aaaagtttga tcaggatctc tgcgacaaga tagagaatgc ctaacacgat aaagacacct
     6601 gccataccaa gcgccattag ctcaaaggaa ctaatcaaat ggtctatatt aattgtcatt
     6661 aggtttcttt cctttcttag ctaaagtaag ctagcaacaa accacctgcg ataacagagg
     6721 cgatttggcc agagacattg gcacctacag catacatgag gataaagttt tgcgggtctt
     6781 catctgttgc cattttttga atgacacgac ttgacattgg aaaagctgaa atacccgcag
     6841 cgccaatcat tggattaatc ttctcttttc ggaagagatt gagaaatttg gcaaacatga
     6901 ccccaccgat agaatccatg ataaaagcaa gtaaaccaaa gacgataatt aagagtgtct
     6961 gtacatttaa gaacaggtct gcctgcattt taatagagat ggtcaatcca agcaagatac
     7021 taataatgtt taccaattca ttttgagcag tttgtgacag gcgatcgagc acaccgcact
     7081 cacgtaataa gttaccgaac atcaaaaagc caactaaagg tagagaaatt ggggcgatga
     7141 accctgctac taaggtaatg atgattggga ataagatttt agtcatttga gatacattct
     7201 cagctttgta agtcatacgg atacggcgct cttttttcgt tgtcactaat ttaatggcaa
     7261 atggttggat aataggaacc agagccatgt aagaataggc tgcaaccgta attggaccca
     7321 gtaaatcctt ggccagttgg ttggcaacaa agatagaagt cggtccatct gctgctccga
     7381 taatgccgat tgaagctgct tctttaatgt caaagccagc cagtacagca acaacaacaa
     7441 caaagaaaat cccaaattgc gctgcagcac caaacaagag catgaatggg ttttgaagca
     7501 agggaccaaa gtcaatcatg gcgccaatac caatgaaaat caacagtggg aaaagttctg
     7561 tttcaatccc aaaattgaag agagcctcaa aaaccccttc ttgctcgact ccgttaacca
     7621 cttgggttaa aactcctgaa ccagggaagt taacgagaat ggttccaagt cccatgggga
     7681 caagtagagt tggttcgtat tcttttttaa ttcctagata cattaagagg gcaccgatga
     7741 ccatcataac aatttgagga atggtgatgg aggttattcc tgcgattaaa gtgtccacaa
     7801 attcacttcc ttttctgtct atttattcta gttatagtac tattaaccaa ttgtaatgag
     7861 accatcacca ggattgacca cttgacctgg accgacgtgg atagctgtaa tggttcctgc
     7921 tgatgaggcc acaatctcat tttccatctt catggcttct aaaatcaata gtggctgatt
     7981 ttcagttact tggtctccca ctgctactaa gacttttaag atggttccag gcattggtga
     8041 tggaatggca tccgctcccg ctgcagctac tggctgtggt gcttgcgctt cccccacttg
     8101 tgggcttgct tcagtaggga caggtactgg agtggatatt ggttgagcgg gggcagctgc
     8161 ttgggcagga gcgccgattt cttccatttc tacaagatat tctttaccgt caatggtaat
     8221 tttaaatttt cgtaacatca tttttctcct atatgttatt gtgtcatgag ttcgtctaaa
     8281 gtatcgttca ccgactacct tttgcaatag atggatgtca cccgaaactg actttctggg
     8341 taaacacctg cagcgatact tgaagcaatt aaagaaacag tgattgcctc agaattgcgc
     8401 ttgagaatgc gtttaacctt gaattgacta ttaggtctat cgccagcagc aatagcactg
     8461 gtaattaaag taaccttttt gtaatcactt ttgtctgcag gaatataagc gggaatcttt
     8521 tcccagtttt cagaaggttc tatttctgta ctaaggggcg aggattcttt caacttctct
     8581 tttttagagt gtttaaacca tttttgtaaa aactgcatga gctacctctt tttcacattt
     8641 acctatttag ggaactagta agtttattat atactaccaa tcttttttga gatggctttt
     8701 ttgtgttctt tctaagagcg ctttcaatgt ttcataaagt ttttcaacag ttacctttta
     8761 ttttattttt aggtaactgt tgaaaaactg agatcaag
//
LOCUS       DATURV010000054         8794 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DATURV010000054 DATURV010000000
VERSION     DATURV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.054"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            537..1064
                     /locus_tag="VCF75_001321"
     CDS             537..1064
                     /locus_tag="VCF75_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEP2546878.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1064..2182
                     /gene="yqeH"
                     /locus_tag="VCF75_001322"
     CDS             1064..2182
                     /gene="yqeH"
                     /locus_tag="VCF75_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEP2546879.1"
                     /translation="MEELFCIGCGIQIQTEVKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGNKDVITTSRFPGTTLDKIEIPLDDGTFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPGKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2207..2515
                     /gene="yhbY"
                     /locus_tag="VCF75_001323"
     CDS             2207..2515
                     /gene="yhbY"
                     /locus_tag="VCF75_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEP2546880.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2584..3216
                     /locus_tag="VCF75_001324"
     CDS             2584..3216
                     /locus_tag="VCF75_001324"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEP2546881.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDYYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3213..3806
                     /gene="yqeK"
                     /locus_tag="VCF75_001325"
     CDS             3213..3806
                     /gene="yqeK"
                     /locus_tag="VCF75_001325"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEP2546882.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            3830..4183
                     /gene="rsfS"
                     /locus_tag="VCF75_001326"
     CDS             3830..4183
                     /gene="rsfS"
                     /locus_tag="VCF75_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEP2546883.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILILDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            4231..4974
                     /locus_tag="VCF75_001327"
     CDS             4231..4974
                     /locus_tag="VCF75_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP2546884.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTKTSKRWFFVAYK"
     gene            5228..6334
                     /locus_tag="VCF75_001328"
     CDS             5228..6334
                     /locus_tag="VCF75_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEP2546885.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFISVQSADYFAQGAIDILCQLGIQQLAFGTENVID
                     YQKLIKVYEKKSKQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            6816..7532
                     /locus_tag="VCF75_001329"
     CDS             6816..7532
                     /locus_tag="VCF75_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2546886.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            7735..8577
                     /locus_tag="VCF75_001330"
     CDS             7735..8577
                     /locus_tag="VCF75_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP2546887.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
BASE COUNT         2857 a         1336 c         1861 g         2740 t
ORIGIN      
        1 ggtaaatcta tgaaaaaagt gtaatcattt tttgatttac agttattatc atgtgttatc
       61 tggcgactgt cctgtttaca agtaagttca caagtatggg ataaaatagt ataaactata
      121 gtaaatactg aatagtcaaa gaaaaatatc ggcataggtc agaagagctg atatgtttaa
      181 tactttttgc ggatatctac tgtatttaaa tgtatttgat atccttgtca aggatatacg
      241 tctaaacaag ttcaaaacga aatcatttgt gattatctta tcaggagttg actcttgata
      301 agatttttgt atatcagtac tgtggtacgt ttagatagct tggcagtgaa tttatagagt
      361 gtcaaacaac gagtgttttg tgttacacta accattagaa gagtctattg ttgagcatat
      421 caaggtgtta tcttttcgag ttgaggtatc atatctagta tatttaacta tggttttaaa
      481 tgctatttca aaaaaaatat gataaaatag aaagtactaa ttattttgga gatgacatga
      541 gtatcgatga ttataggccg acctatatgg tagaggctat ttatgattta agagcaaatg
      601 atttgttgcg tcatggtatt acggctgttt tagtggattt agataatacc ttaattgcat
      661 ggaataatcc tgatggcaca ccagaggttc gcgcttggct tgatgaaatg actatcgcgg
      721 atattagcgt ggttgttgtt tcaaataata aacattctcg tgttgagcgt gccgtttcac
      781 gctttggtgt tgactttatt agccgggctt tgaagccatt tgcttatggt attgaaaaag
      841 ccattgctcg ttatggattt gatcgcaacg aggttatcat ggttggagat cagctaatga
      901 ctgatatcag agctagtcat agagctggaa taaaatcagt tttagtcaaa ccacttgttg
      961 cttctgatgc ttggaatact aaaattaacc gctggcgtga acgccgtgtg atggcaaaat
     1021 tagaagagaa atatggtaaa ttatcatatc aaaaaggaat ttaatggaag aattattttg
     1081 tataggctgt ggtatccaga ttcaaactga agttaaagaa aaagctggtt ttacgccagc
     1141 tgctgctttg aaaaagggta tggaaacagg agaactctat tgtcagcgct gctttagatt
     1201 gaggcattat aatgaaatta cagatgtcca tattaccgat gatgaatttt taagacttct
     1261 tcacgaagtg ggtgatagtg atgctcttgt ggttaatgtg attgatattt ttgactttaa
     1321 tggttctatt attccaggtc tatctcgctt tatttctggc aatgatgtct tactggttgg
     1381 gaataaaaaa gatattttgc caaagtctgt taaggatggt aaagttactc aatggttgac
     1441 ggaacgtgca cacgaagaag gtcttcgccc tttagacgtt atgttaacaa gcgctcaaaa
     1501 taaatatgcg atcaaagact tgattgagcg tatcaatgag ttacgtaatg gtcgagatgt
     1561 ttatgttgtg ggggtcacta atgttggtaa atcaaccttg attaatgcga ttatccaaga
     1621 gattactggt aacaaggatg tcatcacgac gtctcgtttt cctgggacaa ctcttgacaa
     1681 aattgaaatt cctttggatg atgggacgtt catttttgat acaccaggaa ttattcatag
     1741 gcaccagatg gctcattatt tatcccctaa agagctaaaa atagtgagtc ctaagaagga
     1801 aatcaagcct aagacgtatc aattaaaccc agaacaaact ctctttttag gtggtttgtc
     1861 tcgctttgac tttatcaatg gggagcgtca agggtttaca gctttttttg acaatcaact
     1921 agaattacat cgtacaaaat tagctggagc agatgctttt tatgataagc atgttggaac
     1981 tttgttaact cccccaggta agaaagagtt gacagcattt cctaaattag ttcgtcatga
     2041 atttacaatt gatcagaaaa tggatattgt tttttctggt ctaggatgga ttcgtgttaa
     2101 tggtcaaaaa gactcaaaag cgatagttgc tgcatgggct cctgaagggg tcgctgtcat
     2161 tgttcgtaaa gcgattattt aaaaataaaa aagaagttgg aaaaaaatgc ttacaagtaa
     2221 acaaagagct ttcttaaaat ctgaggctca tagcttaaaa ccgattgtac aaattggcaa
     2281 aaatggatta aatgaccaca ttaagactag tattcgtcaa gctttggatg ctagagaatt
     2341 gataaaggtc actttgttgc aaaatactga tgaagatatt catgaagtag cagagatttt
     2401 agaagaagaa attgggtgtg atacggtttt gaaaattggt cgtatcttga ttctctataa
     2461 ggtttctgct aagaaagaaa accgcaagtt gtcccctaaa gttaaagcaa tctagtcctt
     2521 ttatttgtat aggggctggt cttatgagac gaggactatt gtcgcgagaa ttttggagaa
     2581 agtatggcat tagaattgtt gacgcctttt acgaaggtgg aattagagga agaaaaaaaa
     2641 gaaagcaacc gcaaacagat tgggatttta ggagggaatt ttaatcctat tcacaatgca
     2701 caccttgtgg ttgcagatca agttcgccag caattaggat tagatcaggt gctattgatg
     2761 cctgaatgta aaccaccgca tgtggatgcc aaagagacta ttgatgagaa acatcgtctt
     2821 cgcatgttgg aattggctat tgaagatgtt gaagggctag ctattgaaac ctgtgaactt
     2881 gaacgtcagg gaatcagcta tacctatgat acgatgcttt atttgactga gcagcatcct
     2941 gatgtcgatt attattttat catcggagct gatatggttg attatttgcc aaagtggcac
     3001 cgtattgatg aactggtcaa actcgttcaa tttgttgggg ttcaacgtcc aaaatataaa
     3061 gctggaacct cttacccagt catttgggta gatcttcctt tgatggatat ttcttctagt
     3121 atgattaggg attttattaa aaaaggtcgt cagcccaatt atctcttacc taagcgggtt
     3181 ttagactaca ttactcaaga aggtttgtat caatgacata tgaagattat cttccttata
     3241 gcaggaccga actgttagca aaaattgcag agcagatgag ccctaaacgg tttaaacatg
     3301 tacttggcgt ggaaaaggca gccttgtcct tggcagagtg ttatggttgt aacccagaca
     3361 aagcagggtt agcagctctt ttacatgatt atgccaagga gtgccctgac caggtctttt
     3421 tagacttaat tgacaagtac caattatctc cagaattagc taaatggaat aataatgtct
     3481 ggcatggtat ggttggtatt tataagattc aagaagacct agggctaaaa gacaaagata
     3541 tcttgcgagc tattgaaatt cacactgttg gtgctgctga gatgaccttg ctggataagg
     3601 tcttatacgt tgctgattat attgaagaag gtcgcatttt tcctttggtt gatgacgcta
     3661 gaaaaatcgc gaagctcgat ttgaatcagg ctgttgccta tgaaacagtg aacactgtcg
     3721 cttatttagc ctcaaaagcg cagcctattt tcccacaaac tctggatacc tataatgcgt
     3781 tttgcagcta tttaaaggaa tactaatgga taatgatatg aggaataaaa tgaaaaaaga
     3841 agaactctta aaaattgttg tggaagcaac tgatgaaaag cgtgctaaag atattttaat
     3901 cttggattta gaggggttaa ctagtttgac tgattatttt gtgattgcga gtgcaacaaa
     3961 tagtcgtcaa ttagaggcta ttgcggataa tattcgtgaa aaggtaaaag aggctggtgg
     4021 agatgctagc catgttgaag gtaatagcca agcaggctgg gtcttgcttg atttgaccga
     4081 tgtggtggta catctttttt tagaagacga gcgttaccac tataatcttg aaaaattgtg
     4141 gcatgaagcg cctgctgtgg ctctagatgc ctatttagct taatgttaca aagagacctt
     4201 gtcaatacga cagggttttt gaggatgata atggaacata attatgaaaa atttgcgtca
     4261 gtttatgatg ctgtgatgga tgattctcta tatgacttgt ggacagattt ttcacttcga
     4321 catttaccaa agtctaaggg tcgaaatcgt ttgttagagt tggcctgtgg aaccggtatt
     4381 cagtcagttc gttttgctca agcaggtttt gatgtgactg gtttagactt aagtcaagac
     4441 atgctggcta ttgctaaaaa gcgtgcgcaa tctgctaaaa agaaaattga ttttattcaa
     4501 ggtaacatgc tggatttgtc acaggtgggg cagtttgatt ttgtaacctg ctattcggat
     4561 tccatttgct atatgcaaga cgaagttgat gttggcgatg tttttaaaga agtttatgat
     4621 gttttagcaa acgatggcat ctttattttt gatgtgcatt caacctatca aacggatgag
     4681 tgtttccctg gttattccta tcatgagaat gcagatgatt ttgcaatggt ttgggatacc
     4741 tatgctgacg aggctcctca ctctgttgtt cacgaactca ctttctttat ccaagaagac
     4801 gatggacgtt tttctcgctt tgatgaagtg catgaggaac gaacgtatga gttattgact
     4861 tatgatattt tgttggaaca agcagggttc aagtcattta aggtctatgc tgattttgaa
     4921 gataaggagc caactaagac cagcaagcgt tggttttttg tagcatataa atagaagaaa
     4981 aaagtagcct ttttagctat aggatttaat gaattatctt gaatgttact tatatgaaaa
     5041 gagttcatta ggcactaaat gctattatca aagcttagaa gtattggaga cattacgatt
     5101 gacactatgt ctgaaggttg ctaccaactt taaagaaagc attttctatt atacgttgtg
     5161 cttaagcatt tgatttaact cttattagcc ctataaaaga ttatttagta caatttgagg
     5221 agattctatg actgttaccg gaattatagc tgagtttaat ccgtttcaca atggacacaa
     5281 atatttgcta gaaacagcag agggactaaa aattattgct atgtcaggaa attttatgca
     5341 aagaggagag cctgctctta ttgataaatg gattcgatca gagatggctc ttaaaaatgg
     5401 tgcggatatt gttgtggaat tgcccttttt catttcagtt caatcagcag actattttgc
     5461 tcaaggagct attgatattc tttgtcaatt aggaattcag caattagcat ttgggacaga
     5521 gaatgtgatt gattatcaaa aactgattaa ggtctatgag aaaaaatcaa agcaaatgac
     5581 tgcctattta tcgacgcttg aagatacact tagctatcca caaaaaacac aaaaaatgtg
     5641 ggaaatcttt gctggagtta aattttcagg gcaaacgcca aatcatattt tggggttaag
     5701 ttacgcaaag gctagcgctg gaaagcatat acaactatgt cctatcaagc gtcaaggggc
     5761 tgcctaccat tcgaaagaca aaaatcatct gctagcttct gcaagtgcca ttagacagca
     5821 cctgaatgat tgggatttca taagtcattc agtaccaaat gcaggtttac ttataaacaa
     5881 tccccatatg tcatgggacc attatttttc attcttaaaa tatcaaattt tgaatcattc
     5941 tgatttaact agtatttttc aggtcaatga tgaattggcg agtcgtatta aaaaagcgat
     6001 aaaagttagt caaaatattg accacctagt tgacactgtt gctaccaaac gctatacaaa
     6061 agctcgtgta agacgtattt taacctacat tttggttaat gctaaggaac ctactttacc
     6121 taaggggatt catattctag ggtttacctc aaaaggacaa gcacatttaa aaaaattaaa
     6181 aaaaagcagg cctctgatta ctagaattgg agcagaaact tgggatgaga tgacacaaaa
     6241 agcagacagc atctatcaac ttgggcatca ggatatccca gaacaaagtt ttggaagaat
     6301 acctattata ataaaaaatg agaggctcaa ttaaactcta attgagtttt tttgggggct
     6361 tgactctaac attacgttga tggtttatac ttcagttaat ccaatgagga aattttataa
     6421 caataattaa aaaagtcagt caaaagatat gctttcgaag aacaggctaa cgtttttaca
     6481 agattatatc agtaatcatt ttgacaattg tagtaatgga atgttgccaa aatttagaat
     6541 gttgtgtgtt atgaatgaca agattttaaa ggaatactaa ttaaacggga ggtcgtgaac
     6601 cggaagctgt tgtcactcaa gcaccaaaat ctgtctgtaa cataatattg atgtatgaga
     6661 acgcaattat ttggttttta ttgtgatgct taaaacgatt ttatatttct attttaccaa
     6721 agcttttcaa agtgctataa taagggagtt agagtcaata ttagtaacgt taatgttgac
     6781 aatattcaat aaaatactat taaaaggaga caaaaatggg acgtaaatgg gcaaatattg
     6841 ttgctaaaaa aactgctaaa gatggagcaa catcaaaggt ctatgccaaa tttggtgttg
     6901 aaatttatgt ggctgccaaa caaggcgagc cagatccaga gttaaataca gctcttaaat
     6961 ttgtcattga tcgtgcaaag caagcacaag tgccaaagca tgttattgat aaagcgattg
     7021 ataaagctaa aggaaacaca gatgaaactt ttgtagaggg acgctatgaa ggttttggtc
     7081 caaatggttc aatgattatt gtggatactt tgacatcaaa tgttaaccgt acagcggcaa
     7141 atgtacgtac ggcttacggt aagaacggtg gcaatatggg agcttcagga tctgtatcct
     7201 acttatttga taaaaaaggg gtcatcgttt ttgctggtga tgatgctgac agtgtctttg
     7261 agcaattact tgaagcggat gtagacgtag atgatgttga agcagaagag ggaacaataa
     7321 cagtttatac cgccccaaca gatcttcata aaggtatcca agcacttcgc gataatggtg
     7381 tagaagaatt ccaagttact gaacttgaaa tgattcctca atcagaagta gtattggaag
     7441 gtgatgacct tgaaactttt gaaaagctta ttgatgcact cgaatcagat gatgatgttc
     7501 aaaaagtgta tcataatgta gcagattttt aaattgacat agaataataa aaagagttga
     7561 aatctatttc agctcttttt aagtgatgct tttataagta agtaccaggt aacatagtcg
     7621 ttgttagaag ataatgaaca atagtaaatc agtatcaact tgataggaat caattattag
     7681 tgaactctaa ttaaaaatgg taaactagac acaagaacaa aaaggagaga taggatgatt
     7741 aaaaaacaat atttaggaag agcaacaatt gccctagcat caacccttgt gctagcagct
     7801 tgtgggtcat caaaaacagc tgagtcagga aatcagggga gttctaaaga agtcttattt
     7861 gcaacggttg gaacaacagc tcccttttct tatgaaaaag gtggtcaact aactgggtac
     7921 gatatcgagg tggctaaagc agtctttaaa ggatcggatg attacaaggt gtcctttaaa
     7981 aaaacagaat ggtcttctat ctttacaggt ttggactcag gaaaatacca aatgggtggt
     8041 aacaacattt cctttaccaa agagcgttca gctaagtacc ttttttctta tccaatcgga
     8101 tcaacacctt ctgttttagt tgttccaaaa gacagtgata tcaagagctt tgatgatatc
     8161 caagggcata cgacacaagt tgttcaaggg actacaagtg ttgcacaatt agaagacttt
     8221 aataagaaac acagtgacaa cccggttact ctaaaattca ccaatgagaa cattactcaa
     8281 atgttaacca atttgagtga aggcaaggca gatttcaaga tttttgacgc tccaacggtt
     8341 aacgccatta ttaaaaatca agggttagat aatctcaaaa ccattgaact cacctcaaca
     8401 gagcaaccat ttatttattt tatctttagt caagatcaag agaaattgca aagctttgtt
     8461 aacaagcgta ttaaagagtt gactgctgac ggaactctta gcaagcttgc caaagagcac
     8521 cttggcggtg attacgtgcc atcagataaa gagttgaaac ttcctacagc taactaaacc
     8581 atctattaac cagttattca tctagaagac tggtttttct gatcggaaac aatattatag
     8641 tttatgaggt tatgttataa aaattgcaaa tagccttaat caaaatgaga tgttaaagtg
     8701 ctttttgaga agtgaatatt gtaagaagta tgctttgggt tttagtcctc aaataccaag
     8761 ttttttgttg actaaacaaa taaggatggt tagt
//
LOCUS       DATURV010000055         8640 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DATURV010000055 DATURV010000000
VERSION     DATURV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.055"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            1..1967
                     /locus_tag="VCF75_001331"
                     /pseudo
     CDS             1..1967
                     /locus_tag="VCF75_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
     gene            complement(2049..2459)
                     /locus_tag="VCF75_001332"
     CDS             complement(2049..2459)
                     /locus_tag="VCF75_001332"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP2546888.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLNLTGLTAQICQHELDHLEGILI"
     gene            3174..3251
                     /locus_tag="VCF75_001333"
                     /pseudo
     CDS             3174..3251
                     /locus_tag="VCF75_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3349..5226)
                     /locus_tag="VCF75_001334"
     CDS             complement(3349..5226)
                     /locus_tag="VCF75_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP2546889.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRIGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDATNLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKISVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGNYSDYLDEKAFEKETVEADLAKAIVTEEMPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(5223..6431)
                     /locus_tag="VCF75_001335"
     CDS             complement(5223..6431)
                     /locus_tag="VCF75_001335"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEP2546890.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            complement(6556..7404)
                     /locus_tag="VCF75_001336"
     CDS             complement(6556..7404)
                     /locus_tag="VCF75_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP2546891.1"
                     /translation="MKLAVITDSTATLPIDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDRTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            complement(7404..7784)
                     /locus_tag="VCF75_001337"
     CDS             complement(7404..7784)
                     /locus_tag="VCF75_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEP2546892.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            complement(8030..8187)
                     /locus_tag="VCF75_001338"
                     /pseudo
     CDS             complement(8030..8187)
                     /locus_tag="VCF75_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
BASE COUNT         2716 a         1779 c         1379 g         2766 t
ORIGIN      
        1 ctagttttac tctattagtt acagacgttc aagcagatca agttgatgtg taattccttg
       61 gcgtcaatga ttttcacggc gctcttgata ataccggaac agcttacaca ccaagtggta
      121 aaataccaaa tgctgggacg gctgctcaat taggtgctta tatggatgac gctgagatag
      181 atttcaagca agcaaatcaa gacggaacaa gtatacgtgt tcaagctgga gatatggtcg
      241 gagccagtcc tgctaactct gcacttttac aagatgagcc tactgtgaaa gtctttaaca
      301 aaatgaaatt tgaatatggc actcttggta atcatgaatt tgacgaagga ctagatgaat
      361 ttaaccgtat catgacaggt caagcgcctg atcctgaatc aacaattaat gatatcacca
      421 aacaatatga gcacgaagct tcgcatcaaa ccatcgtcat cgctaatgtc attgataaaa
      481 aaaccaatga tatcccctat ggttggaaac cttatgctat aaaagacata gccattaatg
      541 acaaaatcgt taagattggc ttcattggtg ttgtgactac agagattcca aatctcgttt
      601 taaagcaaaa ctatgaacac tatcaatttt tagatgtagc tgaaaccatt gccaaatatg
      661 ctaaagaact gcaagaacaa catgttcatg ctattgtggt tttagctcat attcctgcaa
      721 caagtaaaga tggtgttgtt gatcatgaaa tggctacggt tatggaaaaa gtgaaccaaa
      781 tctatcccga acatagtatt gatattattt ttgcaggaca taatcatcaa tacactaatg
      841 gaactatcgg taaaacacgt atcgttcaag ccctctctca aggaaaagct tatgcagatg
      901 tccgtggtac gctagatact gataccaatg attttattaa aactccatca gcaaatgttg
      961 ttgctgtagc accaggtatc aaaacagaaa attcagatat caaagctata ataaatcatg
     1021 ctaatgatat tgttaaaaca gttactgaac gaaaaatcgg aactgcaact aattcttcaa
     1081 ctatttctaa aacagaaaat attgataaag aatcccctgt cggtaactta gtaacaacgg
     1141 ctcagcttac tattgctaag aaaacttttc caactgttga ctttgctatg accaataatg
     1201 gtggtattcg aagtgaccta gttgtcaaaa attaccggac catcacctgg ggagctgcac
     1261 aggctgtaca accatttggt aatatccttc aagtcattaa aatgactggt caacacattt
     1321 acgatgtcct aaatcagcaa tacgatgaaa accagaccta ttttcttcaa atgtcaggtt
     1381 taacatacac ttatacagat aatgatccta agaactctga tacccccttc aagatagtta
     1441 aggtttataa agacaatggt gaagaaatta acttaacaac tacttacacc gttgttgtca
     1501 acgactttct ttacggtggc ggtgatggct tttcagcatt taaaaaagct aaattagtcg
     1561 gagctattaa cacagatact gaagctttca tcacatatat cacaaattta caagcatcag
     1621 gtaaaactgt taatgctact atcaaagggg ttaaaaatta tgtaacttca aaccttgaaa
     1681 gatcaacaaa aattaatagt gctggcaaac acagtatcat tagtaaggtt tttagaaatc
     1741 gtgatggcaa tatagtgtct agtgaagtca tttcagacct tttgacttct actgaaaaca
     1801 ctaataacag ctttggcaaa aaagagacaa caacaaacaa aaatactatc tctaattcca
     1861 ctcttccaat aacaggggac aattataaaa tgtctcctat tatgacaatc cttgccttga
     1921 taagcttagg tgggctaaac gctttcatta aaaaaagaaa atcctagcgt ttactagtaa
     1981 ttgatcatcc ttatataatc aggcattttc gccaaaaaaa cctaaagtgt cctactctag
     2041 gttttttgtt aaattaaaat accttctaga tgatctaatt cgtgctggca tatttgagca
     2101 gttaggcctg ttaagtttaa acgctttggt cgccaattat ggtccaaata ctcaacagta
     2161 atcttatcat accgctgtgt tttgcgatag ccagataatg acaaacaaga ttctttagtt
     2221 tggtaaattc cctttttaga aaccatcaca ggattaaaca tcaccaaatc aataaatccc
     2281 atactcacaa taacaatgcg cttttgttca ccaatcatat tggcagccat cccaagacat
     2341 tcctgtctat aaaaagctaa ggtatcttgt aaatcttggc caatccatag atcttctttc
     2401 ttggcgactt gcgctttttg ctgtaataaa aaatgatctg ttatgatttc tctaatcatt
     2461 cttatctcct taaaccttct ttttaattca taaactacat gttcaatgga ctattatcac
     2521 catatgatag acttagtatc atctgtttaa aacatcaggt gattccttat ttcaagatat
     2581 cttgggcaac tttatgaaat tctgcaatat gagtaccaaa taaaaattga cctgcatcag
     2641 cagattgttg ctaagttctc atcacaatat tatcgaattt actgtctgct tcttcaaatg
     2701 tctaagagag atagttattg ctttgtgaga ttttcaaacc agatactgct ttctcttgca
     2761 tttacagcct tgataggtcc aaacaaaaca cctgctgttt gaaatgcttt aatagctgat
     2821 atgttgttaa tagttagtat tataaatttt ctgaatattt ctgtcaagtt tttcagttga
     2881 cttaactttt ttacttgatt tttcctcacg taataataga aaagaagcgc cataatgtaa
     2941 actacacccc aaacgttaga taaaaaatct aacgtttggg gtgtttttgt atgacattaa
     3001 gctatgaaga taagctagac atttagcgct taaaaactga gggaactccg tttgaaagaa
     3061 gcatctctgc aaagaagaag acaaaaaacc attataaaga atggacaaag acgagttctg
     3121 attagatttt cttttaagaa cggctaaatt gactcgttct acctactatt atcatcgatt
     3181 attgcaacaa taaacgtatc aaaataaaac gaaaagaact tagtcctcta caatacagaa
     3241 ctaaatcctt tacttcaaat agagtagcca acttttgggg ctcagtgcaa gagcacttct
     3301 tttctattat cacttgttta ttataaacat aacattacca tagtatactt atatgtctag
     3361 tccactcaaa tactcaaacc gttcatacgc caataataaa tcattattgc gctgatcgag
     3421 ttccttttgt aattgagcta actggccata atcactaact acagttaaca tttggttttc
     3481 aatttcttca atattagcct caatagttgc tattttatct tctatctgag cccattcttg
     3541 tttttcaagg taggacatgc gctttttctc ctctttcgga ggaagtggca tttcttcagt
     3601 gactattgct ttagctaagt cagcctcaac tgtttctttt tcaaaagctt tttcatcaag
     3661 gtaatcactg taattcccat aaaaaatacg aatatcgcct tcttcaaagg ctaaaatctt
     3721 agtggctact ttgtctagga aataacgatc gtgactaacc gtaatgactg gtcctgcaaa
     3781 gttagctaaa aagttttcta agaccgtcaa ggtggcaatg tctaaatcat ttgttggctc
     3841 atcaaggaga agaacatttg gcttttcaat gagcagtttt agcaagtaga gccgtttttt
     3901 ctccccacca gataatttag caatcagcgt gccatgactt gatctcggaa aaagaaattg
     3961 ctctaaaagc tcactgatac tcgttgttcc cacgcttatt ttaacctcat cagccacttc
     4021 ttgaaggtaa ttaatgaccc gtttggtctc atccatatct ttgagctgct gagagaaata
     4081 acctacacga atagtatctc caatatcaag cttcccagaa gttggtttga gatcaccgtt
     4141 gatgatattt aacagggtag attttccgac gccgttatct ccaacaattc caatacgatc
     4201 tttattttga ataatcaagt taaaatcctt aatcaactgt cggtcgccat aagcaaaaga
     4261 aaggtcttca aagtgaatca ccttcttccc aattcggctc gtttcgaaag ccatctccaa
     4321 tttatctgca gaactgtctt ggtgaacttc ttttttcaag tcagtgaaac gattgattct
     4381 agcctgctgt ttggttgcac gcgcctgtgg ctgcgtcctc atccaagcta attcttgttt
     4441 atagagttgc ttcttcttgt gtaaattagt agcatctcgt tcgtcttgtt ctgcttttaa
     4501 gcgcacatag tcttggtaat tgccttgata ttctgtcagt cccgctttat ccaattcaaa
     4561 aatacgggta gccacatgat caaggaaata acggtcatgg gtaataaaaa ggacggtttt
     4621 tttagctgtc ttgagatagg ttgttaacca agcgatggtg tcaatatcga gatggttggt
     4681 cggctcatcc aacaagagta agtcagcagc tcctagcaat acttgagcta actggacacg
     4741 tcttctcata ccacctgata ggtctcccac tttttgttcc aaatccgtga tgcctaattt
     4801 tgataggact gttttgacat cactttcaat agaccaagca tctaagcgat ccatttcaga
     4861 catgagggac tctaaggtct cttgtttatc ttcagtataa ttcgccatca atagttcata
     4921 ctgacgaatc agctgaatcg ccttaacatc agatgacaat accgtatcta aaacagagga
     4981 tgcatcgtta aattcaggat cttgtgttaa gtaagcgatt ttgtaatcat tggctttaga
     5041 aaatggtgaa tggtctccgt caaaaccaat ccttcccgac aagacatcta aaagagttgt
     5101 ttttcctgtt ccattaaccc caataatccc aatacggtca aagtcatgaa taataaagga
     5161 aatatcctga aagactgttt tatctcccac cgtttttgtc aatttctcca ccaaaaaatg
     5221 gctcatgttg ctaattcctc tctaacaaat gcttcaattg tagtgaaatc attatctagg
     5281 cgtccctcta caatcgctaa ttcaactttc tcaagcataa gccctagctg aggacctggt
     5341 ttcatcccaa aatccttaat caggtgagaa ccattaagga cgatatcgta tttgttatga
     5401 atggctagcg cttgatctaa agcttcaatg tggtccatgt caacagataa gctttgagct
     5461 tttcggaggc cttccactag acttgccatt tccttgccgt actggtaaat atccaatttt
     5521 tcaaaagatc tttcctgacg aattctataa agagcaatca actttgtgac ataacgctga
     5581 aaatcatttg aagtcttcca tgcttttagg aagcttttag gatcctcaat ggctaaacta
     5641 atcatcacat aagcccaagc ttggtggtga tcactaaagg taaagttatc tttcaaagaa
     5701 actatcagat gattcaagcc tgcttcctgc tgcttcaagc ctggtaaata gtcataagcc
     5761 tgacaagcaa tcatggcaga aatccctttt cgccagtgcg gtgccatcaa taatttatca
     5821 aattcagtaa aagaacgttc cacagaaatc ttttccaata gaggtgaatg gctcctcata
     5881 gcttcaaaag ttgcagcttc aatgtcaaaa tccagactcg ccgcaaatcg aaagcctctc
     5941 ataatgcgta gtgcatcttc ttcaaaacgt tcctctgctt tacccactgc tcgtaaccgt
     6001 ttttgctcca aatcaatgag tcctctaaat ttatcaataa cttgaccatt ttcatccaag
     6061 gccaaggcat tgacggtaaa gtctcttcgt tttaagtctt cctctaaaga gcgcacaaaa
     6121 gaaacctgac taggacggcg atagtcaaca tagacatctt ctgtcctaaa ggttgtgatt
     6181 tcatactcac cgccattttc aagcacgagc actgtaccat gttcaatgcc aacatcgaca
     6241 gttctgttaa aaatcgcttt agtctcttca ggataggaac tagttgcaat atctacatca
     6301 tgaatgggac gctcgagtaa aacatctctg acgcttcctc ccacaaagta ggcttcataa
     6361 ccagcttctt ttattttagt caatatgggt aaagccttct gaaactcaga aggcattgtc
     6421 attaatttca tacgttgttt tctaacctat catccaacta gcccaatcta aagtgtgatt
     6481 aggctacttg ggttctatca cattttgtta ttgttttagt gattcttgca attaactatt
     6541 ttttctaatc gacaattaaa gcctcggtgt tacgccaaat gcaatggctc cttctcctaa
     6601 atgagtagca atcaccgctc caaaatgaac ctcttcaatg tcatattgat aaccactgtc
     6661 ttgtaagagt cgtttcaaat aatcagcttt gtcttgtgct tttgagtgaa taatagacac
     6721 attatactga ccgtctgcta tcaagtcatt taaaatctca accaagcgtt tcatggcttt
     6781 cttttctgtt ctcacttttt cataaacaac aatttttccc tcttcatcaa agcgtaaaat
     6841 aggtttaata ctcaaaagat ttccaagaag agcagaccca tttgacaggc gtccgccttt
     6901 aaccagatga ttaaggtcat caaccataat aaaggctgtc gtcctatcaa tctgttcttg
     6961 cagcttgttt aaaatagctt gaaatgtcat tccttgacgt gaccaatcta gcacattttt
     7021 taccatacta cctaaaggag cagatgtgat cttactgtct ggaaatgcca tttcaatctc
     7081 aggatgttct tcagctaaaa attgaatatt ttgccaaaag cctgagattc ctccagctaa
     7141 gaacaagcca ataacatgtg tataaccctt agaagacaaa agtcctaaaa gattatctag
     7201 ttcagataaa cttggctgac ttgtcttggg aagattttgg gagtcagcca tcttttgata
     7261 aaaatcatct atactcaaat ttcgaccttc aaaataggtc tcatcgtcaa tgataactgg
     7321 tatatcaagg ctaaaaatag ctttatcttg tttcagatct attggtagtg ttgctgtact
     7381 gtcagtaatt actgctaatt tcattaattt ttaaactcca aatgaatacc tggacgatcc
     7441 aaggcaattt ctgtgacttc ataagtcaat cgcttaacca tgtctagcaa aggagcagct
     7501 aaggattcaa cttcctcttg cgtaaattcg cttggtttat ccactaaacg atctaaaatg
     7561 cgaaccattt gtgagatgac cccactaata acaaattctt ctttgacaat aacaaattgc
     7621 aatacagata caattactgt ttctttttgc tgctcatctt tatcaatcaa ctgaaaagtc
     7681 acttcaaaat tggtctctgg tgttccgttt tctttttccc actctaaatt tctagcatca
     7741 tagtggtatt gattgacaaa ttctttttca cgaactaatt gcatttttta ttctccttgg
     7801 tatcaatatg atccattata gcatagattt gaagattatt aaagtagccc ctaaactact
     7861 attttcgcat aacgttttaa acagtgctat ctctcgaaca gatatctatt ttttcaccta
     7921 tttatattta gtcgtcctta tactgacatt atatcaaaaa acacccgtta gtggtcttgt
     7981 actgcacctc aattaaaaca gaaccactgg tcaggtaagt tttaagtcaa ggataaaaag
     8041 gtttatcatt aaggcctcat catgcttagc caagtatttc gaactagctt gtcttagctc
     8101 tagtcgctca aacgtaaatc ctaggcgttc aatccgccgc aaaacctctc caactaaccc
     8161 atacttaaac ccatcgggct taatcatcgc tccgcccttc ttcttttatc cattataaaa
     8221 taaaaacgtt ttaacagaca aaaaatagga aagtaccaga ctattggatt tattttttta
     8281 tctcaaactc cattaacgat gttgagttaa aggcattcaa aacaacatag atcgtaagct
     8341 aacaagattt tttatcaatt gaacctatcg tccccagtta taccacgacc tcttctggat
     8401 gaggtctttt tttatttgtc taatttcatc aacgcttctt cccttctttc ttctatcaca
     8461 gctctcctat tgctcacttc ctcatgcagc aacatatcat caaaaaaccg ttcattttta
     8521 agcacaacca ctactgtatc tcttgtgatg tctccattgc aatcgcttgt ttaattttat
     8581 taaaaattat ttaaaaaatt aaatattatt ttaagaactt gcaataatat atgtctatat
//
LOCUS       DATURV010000056         8538 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DATURV010000056 DATURV010000000
VERSION     DATURV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.056"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..1443
                     /locus_tag="VCF75_001339"
     CDS             <1..1443
                     /locus_tag="VCF75_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEP2546893.1"
                     /translation="IYSTFLTLLLTVILYANSLNLPLKFISLLFVVSTLLIVFLPKKL
                     EVATIIFILVFGTISAIISPINDIPDEYVHYSRTVYISEGDINLTNNNKKLRISKDVD
                     KLIKQSGKTFITSNLKATKHSTREYSYPYIKGTNAYYSFSYIPQALGILVGNALDLPI
                     LLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLPMNIYLAASFNQDGFAIGL
                     VLVTIGLFINLLSSKDKSNYNTKFFLYLVLCGLLVLSKFTYFLLVCLPLFIPNEKFGK
                     NTKLVILKKLGGLLLIFLFAAMWFRLYGQVKTPYVADFLKEVNVSQQVKNMLESPIVY
                     SSIIIRHMVINLINMNNIFQFGALSYGITNLFPLYVCFFFFVYISNASKITINIVEKM
                     GIIFVISAIIGATVLAMYLTWTPVGSSTVLGVQSRYLIGIIPLVLLLFSSQQQKFKQI
                     EDILSDKLAIHISLLFILAMLMSTIFRYYH"
     gene            1537..2760
                     /gene="pepT"
                     /locus_tag="VCF75_001340"
     CDS             1537..2760
                     /gene="pepT"
                     /locus_tag="VCF75_001340"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEP2546894.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALQLAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            2801..3289
                     /locus_tag="VCF75_001341"
     CDS             2801..3289
                     /locus_tag="VCF75_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEP2546895.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHVEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(3276..3473)
                     /locus_tag="VCF75_001342"
     CDS             complement(3276..3473)
                     /locus_tag="VCF75_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEP2546896.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            3522..3998
                     /locus_tag="VCF75_001343"
     CDS             3522..3998
                     /locus_tag="VCF75_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP2546897.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKTSSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            4013..4693
                     /locus_tag="VCF75_001344"
     CDS             4013..4693
                     /locus_tag="VCF75_001344"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEP2546898.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPISFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWV
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    4717..4840
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            4855..5385
                     /gene="infC"
                     /locus_tag="VCF75_001345"
     CDS             4855..5385
                     /gene="infC"
                     /locus_tag="VCF75_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEP2546899.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            5427..5624
                     /gene="rpmI"
                     /locus_tag="VCF75_001346"
     CDS             5427..5624
                     /gene="rpmI"
                     /locus_tag="VCF75_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEP2546900.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            5683..6042
                     /gene="rplT"
                     /locus_tag="VCF75_001347"
     CDS             5683..6042
                     /gene="rplT"
                     /locus_tag="VCF75_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEP2546901.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(6333..8504)
                     /locus_tag="VCF75_001348"
     CDS             complement(6333..8504)
                     /locus_tag="VCF75_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEP2546902.1"
                     /translation="MKKFKTLITGFINTRLGFIITLLFCYWIKTLWAYHTDFSLDLGN
                     IYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNILLISNSIYYREFSD
                     FITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSITKKIKNDQRPFNKR
                     AAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFTLYSGNQTYQAQ
                     KERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLESFQQFLIDYKLKEG
                     DKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSLFGLNSGSFMVNYG
                     GENTQFATPSILAQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYFFDSSYFSKQNSKN
                     SFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGESSEEGFPLAKTDD
                     ETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGISNSRNSSLAPLLGK
                     DSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLLHILGIDTSQFVQL
                     GQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNPDEMTIAKTKEIRS
                     AVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKDISAKLGSESTSLY
                     SKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNTKNRE"
BASE COUNT         2780 a         1383 c         1635 g         2740 t
ORIGIN      
        1 atttattcga cttttttgac attacttctg acagttattt tatatgccaa tagtttaaat
       61 ctacctttaa aatttattag tcttcttttt gtggtgagta ctttactgat agtgttttta
      121 ccaaaaaagt tagaggttgc tacgatcatt tttattttag tatttggaac gatatctgct
      181 atcatatcac ctataaatga tattcctgat gaatatgttc attattcaag gacagtttat
      241 atctcagaag gtgatattaa tcttaccaac aataacaaaa agttaagaat atccaaagat
      301 gttgataagc ttattaaaca gtcaggaaaa acatttataa catcaaactt gaaagcaact
      361 aaacacagca caagagagta tagctatcct tacatcaaag gaactaatgc atattatagt
      421 ttttcttata taccacaagc tttgggtatt ttagtaggaa atgcgctgga tttacctata
      481 cttttaacat attatttcgg aagattgtgc aaccttatta gctacgctat gcttgctttt
      541 atagctatca agttgtctgg ttcttttaaa caagtgatag ctgttgttac tttactacct
      601 atgaacattt atttagcagc atcttttaat caagacggct ttgctatcgg tttagtttta
      661 gtaacaattg gattgtttat taacttgctc tcatcaaaag ataaatctaa ctataatacc
      721 aaattcttcc tttatttagt tctatgtggg ttactagttc tttcaaaatt cacatacttt
      781 ttattagtat gccttccatt atttattcct aatgaaaaat tcggaaaaaa taccaagtta
      841 gtcatcttga aaaaattggg aggactatta ctcatttttc tttttgctgc aatgtggttt
      901 agactttatg gacaggttaa aaccccatat gtcgcggatt ttctaaaaga agtaaacgtt
      961 agccagcaag ttaaaaatat gctagaatcc cctatagtct atagcagtat tattattaga
     1021 catatggtaa ttaatctgat taatatgaat aatatttttc agtttggcgc attaagctat
     1081 ggtataacga accttttccc gctttatgtg tgtttcttct tttttgttta tattagtaat
     1141 gctagtaaaa taactattaa tattgtagaa aaaatgggta taatctttgt tatttcagcg
     1201 attataggtg caactgtatt agctatgtac ctgacttgga caccagtagg gtcctcaact
     1261 gtattaggtg ttcaaagtcg ttatctgata ggaattatcc cattagtgtt gcttcttttt
     1321 agtagtcaac agcaaaagtt caaacaaata gaggatatcc tttctgacaa actggctatt
     1381 catatttcgc ttttgtttat tctcgcaatg ttaatgagta ctatttttag atattatcat
     1441 taaactctta attagaggct ttaaaaaact tagcttgaat aagtatttta aagcctttta
     1501 tgctataatt aaattgagaa attattgagg tggcctatga agtacgataa cttgttagat
     1561 cgatttataa aatatgtcaa agtgaataca agaagcgatc cagacagtga gactactcct
     1621 agtacggaga gtcaagaagc atttgcctta acgattttaa agcctgaaat ggaggcaatt
     1681 ggtttacaag atgttcatta taatccagtt aatggctatc ttattggaac tctgcctgcc
     1741 aataatccaa ccttgacgcg taaaattggt tttatagccc atatggatac ggctgatttc
     1801 aatgctgaaa atgttaatcc gcagattatt gacaattatc aaggagggga cattacactt
     1861 ggcagttcta attataagtt ggatccaaaa gcatttccaa atcttaataa ttatatcggt
     1921 caaacattga ttaccactga tgggactaca cttttagggg cagatgataa atctggtatt
     1981 gctgaaatca tgacagcaat agaattctta acatcacaac cacaaataga gcactgtgac
     2041 attaaagtgg cttttggccc tgatgaagag attggggtcg gagctgataa gtttgaggtg
     2101 gctgattttg aggtggattt tgcttatact atggatggcg gtcctttagg cgaattgcaa
     2161 tatgaaactt tcagcgctgc agctctagaa gtgacttttt taggcagaaa tgttcatcct
     2221 ggtacggcta aggatcaaat gattaatgcc cttcagttag caatagattt tcatgagaag
     2281 ctacctgcta aagatcggcc tgaatacaca gatggttatc aaggtttcta tcacttaacg
     2341 ggactaactg gaacagttga agaggcacgc gcatcttaca ttattcgtga ttttgaagaa
     2401 gcatcatttg aagctagaaa agtgaaagtt gaaaatatag ctcaatcgat gaatgctcag
     2461 ctaggaacaa aacgtgtctt ggtagagctt aatgaccaat actacaatat gaaaaaagtt
     2521 atcgaaaaag atatgaccgc tattgagtta gctaaagaag taatggaaga gcttgctatc
     2581 aagcctgtaa tcgaacccat acgtggagga acagatggct ctaaaatttc atttatgggt
     2641 attcccactc ctaatatttt tgcagggggt gagaatatgc acgggcgttt tgagtttgtt
     2701 agcttacaaa cgatggagag agctgtcgat gtcattattg gacttgtttg caaagcctaa
     2761 ttatcatgga attgatattt agtggaaggg gcttataaaa atgattaaac tatttggtaa
     2821 aataaggtat cactggcagc cagagttgtc ttggtctatt atttattggt ctattgcgtt
     2881 tgcacctatt tttgtaggtt tatctttgtt gtatgagcgc actgaaatac ctagtcgggt
     2941 ttttattcta tttgctattt ttgctgtcct agttggcatt ggattacatc gttatttcat
     3001 tattgaaaat aatggtattt taagaatcgt atcttttaaa ttgtttggtc cacgtaagct
     3061 acttatttcc actattacta agattgaggt gaccaaatca acactttgtc tgcacgttga
     3121 agataaaagt tatctctttt atatgcgcaa atggcctaaa aaatattttc ttgatgcctt
     3181 agcggttaat ccatacttcc aaggagaagt tatattaagt gataacttta ttaaattaga
     3241 ctattttgaa gtttatcagc atgataaaaa agcccttact cgagggtaag agctttagtt
     3301 gggcaagatt tgacagctaa aatagcgtcc ttatctgaag gacaaattga ctgacttgtt
     3361 tcagatgagc ttgaaaatgt gacgatacca ttgtcatgat aatcaaaaag tgatgaatag
     3421 gtttggcata gaccacaagc gatgcatttt tctggaatga tagatacttt cataactatt
     3481 attctaatat gattttgtta aattaacaag gggggacgat catggctaaa gaaccatggg
     3541 aagaaaaaat tgttgatgat actataggga cacgaacacg taaatcaaga aatgctttca
     3601 ttagcacgcc ttggttgact gctttattaa gtgtattctt tgtcatcatt gttgctatac
     3661 tttttatttt cttctataca tcaaatagcg gtagtaatag acaagctgaa acaaatggtt
     3721 tttatggagc atccactcat aaaaaaacaa ggaaagcttc taacgctaaa aaaacatcaa
     3781 gtagttcaac aactacagac acaacacctt ctagcgaaga aacacttgct tctagtgaag
     3841 gaactggcga aacccttact gtattggcag gtgagggggc agcttctatt gcagctcgtg
     3901 caggtatttc tgtggagcag ttacaagcac ttaatccaga gcacatgact caaggatatt
     3961 ggtatgccaa tccaggagat caagtcacta ttaaataatt gaagaggaaa aaatgaaagc
     4021 aattaaaatt gcgattgatg ggccagcttc aagtggaaaa agtacagtag ccaagattat
     4081 tgcaaaaaat cttggctata cttatctaga tactggtgct atgtatcgct cagcaactta
     4141 tatcgcactg acacatggct atacgggtaa agaagtagcc cttattttag aggaacttga
     4201 gaaaaatcct atttccttta aaaaagcaaa agatggctcc caattagttt ttcttgggga
     4261 tgaggacgtg accttagcca ttaggcaaaa tgatgtgact aacaatgttt cttgggtctc
     4321 agctttgcca gaaattagag aagagctggt tcatcaacaa agacgcattg cacaagctgg
     4381 tggcattatc atggatggtc gtgacattgg aacggttgtt ctcccagatg cggaactaaa
     4441 aatcttttta gtggcttctg tcgaagagcg ggcagagcgc cgctataaag aaaacctaga
     4501 aaaaggaatt gaatcagatt ttgagacgct aaaagaagag attgcagctc gtgattacaa
     4561 ggatagtcat cgtaaggttt cacctctaaa agcagctgaa gatgccctta tttttgatac
     4621 cacaggtgtt tctattgatg gcgtggtgca atttatccaa gaaaaagcag aaaaaatagt
     4681 tgacatgtcc tagagcctat gttagaatag taacaatgag aaaagtagaa gtgagagctt
     4741 ctcgccttgc gacttaggtt gtctggccct aacatgtcaa attcctatgg aatattatat
     4801 gtgcgggctt gatttatcaa gtccgctttc gtttttctct aaaaaaataa agaggtgaag
     4861 atcatagcta aaaaggatct attcattaat gatgaaattc gcgttcgcga agttcgtctc
     4921 gttggtcttg aaggtgaaca attaggtatt aaaccattgt ctgaagcgca atcattggct
     4981 gatgcttcaa atgttgattt ggttttaatc cagccacaag ctgttcctcc tgttgccaaa
     5041 cttatggact atggaaagtt caaatttgag tatcaaaaga aacagaaaga acaacgtaaa
     5101 aaacaaagcg ttgtgactgt gaaagaagtt cgtctcagtc cagttatcga taaaggtgac
     5161 tttgaaacaa aacttcgtaa cggtcgtaaa ttccttgaaa aaggaaataa agttaaggtt
     5221 tctattcgtt tcaaaggacg tatgattact cataaagaga ttggtgccaa ggtactagct
     5281 gactttgctg aagcgactca agatattgct atcattgagc aacgagcaaa aatggatgga
     5341 cgccaaatgt ttatgcaact tgcaccaatt tcagacaaga agtaattgtc acagtttcat
     5401 tatatcttta agaggagaat actaaaatgc caaaacaaaa aacacaccgc gcatcagcta
     5461 aacgttttaa acgtacaggt tcaggcggtt tgaaacgctt ccgcgccttt acatcacacc
     5521 gtttccacgg aaaaactaaa aaacaacgtc gtcatcttcg taaagctggt ttggttagct
     5581 caggagattt caaacgtatc aaagcaatgg ttactggtct ttaattttaa aggcagtaga
     5641 catttacgag tattataaga attatttgaa ggagaactat taatggcacg tgttaaaggt
     5701 ggcgttgttt cacgcaaacg tcgtaaacgt attttaaaat tagctaaagg ttactatgga
     5761 gcaaaacaca tcttgttccg tactgcaaaa gagcaagtga tgaactctta ctactatgca
     5821 taccgtgatc gccgtcagaa aaaacgtgat ttccgtaaac tatggatcac tcgtatcaat
     5881 gcggctgctc gtatgaacgg tttgtcatac tcacaattga tgcacggttt gaaacttgct
     5941 gaaatcgaag taaaccgtaa aatgcttgct gatttagcag tagctgatgc agcagctttc
     6001 acagctcttg ctgatgcagc caaagcaaaa cttggcaaat aagaaacgct atacctaaaa
     6061 aataactttc tattacctat agaaagttat aaagaaacaa aaatgaagga gacgatggac
     6121 gtcttctttt tattatactc aatataataa aaagaagttt cccatgtttt attaccagta
     6181 atgtgggata tttagatggt agcaagaagt tttatagttg atttgttttc tttaggtcta
     6241 attagcatat tttgattact gataaacttg aatatcgcca ataaaaaaga agcaaaatta
     6301 ttattttgct tctagtcaga tcattaaatt gattattctc tatttttggt gttatcctct
     6361 tttagttcag aagttgccgc gtcagcttct acgggattta gttctaagta agatggtgct
     6421 ttaaaaagtt tctgagttga tttatgacca tttttactgt ataaacttgt tgactctgat
     6481 ccgagttttg ctgaaatatc tttcagttgt tttaactgct tagtgtagat aaactggtta
     6541 ggatcaatag cttttagacc attttgagta tcaaagcgca ggagatcacc cgtttgaata
     6601 gcgtcgctag ctgctagttg ttgagcaaca gcagagcgaa tttccttagt tttggctata
     6661 gtcatttcat cgggattagt gatttctaaa cctgtttgcg tgttgtacag acgtccacta
     6721 tagttagtgt attcaggagt catataagtc ccagatgttc gttgtgccac aatctggcta
     6781 ttttgaggtg ataataaatc ttgtcctaat tgaacaaact gactagtgtc aataccaagt
     6841 atgtggagta aagtaggaag agcatcgatt tcaccgccaa aggtttcttt gatactacca
     6901 ttcgtataac ctggaatatg gatcatatac gggactcgtt gtagcatagc attatcatat
     6961 tcagaccaag tttcagaatc tttaccaaga agtggagcaa gactagaatt acgagaattt
     7021 gaaataccat aatggtcacc atataaaaca aaaatagaat tgtcgtacaa accagtggct
     7081 ttcaagtaat caaaaaaaga tttaagggca gcgtctaaat aattggcagt agcaaagtaa
     7141 ccattgatcg tttcatcgtc tgttttggct aaaggaaaac cttcttcact actttcgcct
     7201 tttagactag tataaggata gtgattactc acagtgataa acttggtata aaaaggttgt
     7261 tgcatctgtt caagatattt tatggaatcc ttaaacatgt acttatcatt taacccatat
     7321 tgaaaagagt tcttactatt ttgtttagag aagtagctag aatcaaaaaa ataattatag
     7381 ccccattgtt tataagcatt attgcgattc cagaaagttc caacgttacc atgaaagaca
     7441 gcgctggtat agccaccttt ttgggctaaa atacttggag tagcaaattg tgtattttca
     7501 ccaccgtagt tcaccataaa agaaccacta tttaaaccaa ataaggaatt ctccatcatg
     7561 gtttcagcat cagaagtttt acctgcttta acctgatgaa aaaagttagg gaatgctaag
     7621 gtagcatttg agtgataaag cgagttgata aaaggcgtta cctcatattc tttatctcct
     7681 tcttttagtt tataatctat taagaattgt tgaaagcttt ctaaatgaag aacaataata
     7741 tttttgcctt ttccaattcc aaaatactgg ggatcaggtg ctgcataatg tcctttgaca
     7801 taagttttaa catcaattaa ttcttctgca gtagctccat ttcgttcttt ttgagcttga
     7861 taggtttgat tgccgctata gagggtaaac gctggtaatc ctaaagctct cacaatataa
     7921 gtatttgaaa agccacgtgt taatagttcc ggacgatcaa tttctgctaa aaaaagatta
     7981 atagaaagca atagactaga taaagcggtg atggcaaatg ctgcgcgctt gttaaaagga
     8041 cgttgatcat ttttgatttt tttagtgata gataaactaa ttagaataat aaaatcaaga
     8101 atatagataa tatcccagat gcgtaaaagg tttaaagcag agtcccctaa tccagcggag
     8161 actttactgc tagctagcat ggcactcact gtaataaagt ctgaaaattc tcggtaatat
     8221 atagaatttg atatcaagag aatgttaagg ataatataaa ccacccaaga acagatataa
     8281 aaggctcggg tattcttaac gtaaagagcg acccccaaaa gtaaaaaagc tagtgggatt
     8341 ggattgataa ttgttagaaa gacttggtat atatttccca aatctagtga aaagtctgta
     8401 tgatatgccc acagagtctt tatccagtaa caaaagagta gagtaattat aaaacccaat
     8461 cttgtgttga taaaacctgt gataagtgtt ttaaatttct tcacagtagc aacttcctta
     8521 tcgttttttc ctaaagat
//
LOCUS       DATURV010000057         8460 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DATURV010000057 DATURV010000000
VERSION     DATURV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.057"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..89)
                     /locus_tag="VCF75_001349"
     CDS             complement(<1..89)
                     /locus_tag="VCF75_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2546903.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLK"
     gene            complement(191..289)
                     /gene="ffs"
                     /locus_tag="VCF75_001350"
     ncRNA           complement(191..289)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VCF75_001350"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            489..1721
                     /locus_tag="VCF75_001351"
     CDS             489..1721
                     /locus_tag="VCF75_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEP2546904.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANMPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDHLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEPLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(2335..2850)
                     /locus_tag="VCF75_001352"
     CDS             complement(2335..2850)
                     /locus_tag="VCF75_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEP2546905.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(2850..3629)
                     /locus_tag="VCF75_001353"
     CDS             complement(2850..3629)
                     /locus_tag="VCF75_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP2546906.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGINSKNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(3769..4308)
                     /locus_tag="VCF75_001354"
     CDS             complement(3769..4308)
                     /locus_tag="VCF75_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEP2546907.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(4312..4623)
                     /locus_tag="VCF75_001355"
     CDS             complement(4312..4623)
                     /locus_tag="VCF75_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546908.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(4841..5983)
                     /gene="tgt"
                     /locus_tag="VCF75_001356"
     CDS             complement(4841..5983)
                     /gene="tgt"
                     /locus_tag="VCF75_001356"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEP2546909.1"
                     /translation="MTDYPIKYRLIKTEKHTGARSGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVVKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            6202..7065
                     /locus_tag="VCF75_001357"
     CDS             6202..7065
                     /locus_tag="VCF75_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEP2546910.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGLILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGSSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
     gene            7151..>8460
                     /locus_tag="VCF75_001358"
     CDS             join(7151..7601,7601..>8460)
                     /locus_tag="VCF75_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP2546911.1"
                     /translation="MKFNQETKVKIYELRQMGESIKSISKKFDMAESDLKYMIRLIDR
                     YGVTIVQKCKNNYYSPELKQEIINEVLIDGQSQKQTSLDYALPTSSMLSRWIAQYKKN
                     GYTILEKPRGRPSKMGRKRKKNLEEMTEVERLQKELEYLRAENAVLKKPERIPLEGRS
                     KAQRATEIIQALRNQFPLEMLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDE
                     HKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMSLAARIRRKRKYSSYKGEVGKKADN
                     LIKRHFEGSKPYEKCYTDVTELALPEGKLYLLPVLDGYNSEIIDFTLSRSPNLKQVQT
                     MLEKTFPADSYSGTILHSDQGWQYQHQSYHDFLESKGILPSTSRKGNSPDNGMMDSFF
                     GILKSEMFYGLETTYQSLDKLEEAITDYIFYYNNKRIKAKLKGFS"
BASE COUNT         2666 a         1729 c         1557 g         2508 t
ORIGIN      
        1 tcttttaaat tttcatctgc ataagctaat tgacttccat agctaaaaac acatgataat
       61 atgataattg tcaaaatgtt tgttttcatt taaaactttt tatgtcctgt tttatacctc
      121 ttaacctaaa taaaaatatc aatcggtttc aacaactgat gacactatag aaggaagaga
      181 ttactttagt aaagtcaaca aaaaaagagc acacacccga aaatcgctta gggctgctgg
      241 attcctcccc tgacccactt cacgcacaag tgttgctccg cctattatta tatcatatta
      301 cggacagttt gcaagttttc caaataagta aaatcacttt caagtgagca tagcttcatt
      361 ttgggacaag atggactgaa tacctagaaa aatcatgacc ctttccaaac acgattttgc
      421 tttttgaaaa tctttccctc tccccattcc agctattctc tatgttataa taagattatt
      481 agattagaat gggagttatg atgaaacaaa agattaaaat cttaacagta attggcctta
      541 tgacagttgg tatgtctgcg tgtcacaaca ctagcaaacc ttctaatacc gattctgttt
      601 tttctttaac cggaaaaaag cgccaacaaa tcgtcaaaca agtaagacaa cggtattatt
      661 tccaacaatt atccaaaacc gaacaagaaa attacctcac tttatacgat agtctagcac
      721 aatttcgtga aatcatcagt ttgacgccgg cttctaaaaa aagcttaatt aaaacgattg
      781 acgcctttgt gatggataat cccgaatttt actggattac atctgccgat tatcgatttg
      841 agttttctga tcaaacggtc tttgtcacct ttcctatccc agaagatgcc aagaacgttt
      901 atcaagactt gcaagcaatc ggtaatgata tcgtcgcaaa catgccatca aaggatcgct
      961 atgagcaggt caaatatttt tatgaggtca tcattcgaga cacagactac aataaaaaag
     1021 cctttgaagc ctatcaatca ggcagtcaag cccaggtcgc ttctaatcaa gacattaaaa
     1081 gtgtttttat tgatcattta tctgtttgta atggctatgc ccaagccttt cagtttcttt
     1141 gtcaaaaagc tggcattcca gtcgcctata tccgtggaac tggtacatct cagcaacctc
     1201 agcaatcttt tgcacatgca tggaatgctg ttcaaattaa taacacttat tatggcgttg
     1261 atgtcacttg gggtgaccct gtttttgata accatctctc acatcaaaag caaggaacta
     1321 tcaattacag ttttctatgc ctgccagacc atctaatggc cttatcacat cagccaagca
     1381 aagacattgc ttttaatact aaagaacgtt ttgaaaatgt ttggactata ccttcttgta
     1441 cagatgattc tttgctttat tctaaacgtc atcaaagcta tatctcaacg tttgacagtg
     1501 acgccatcct tgcaagcctt gaaaatcagt tgttaaatag acaagagcca ctgtctctac
     1561 aatttgctca tcaagatgat tatcaacaaa tggtaactga tttaacgacg aatcaaacag
     1621 gttatcataa ccttttcaat cagtattgga ataattatac gggctttacc tatggactct
     1681 taccagagac tcttagtatt tcatttgcta gtaggaacta ggtttagaca catgaaacac
     1741 aaagttaaga tgattatctt tgtcaaacat tacaagaaat tgatatacta acacaattga
     1801 aggcgattat tattctaacc gtcttcaata gatataacct tttcagattg agcacatcta
     1861 atgcttgtaa cattagcaac agtgatgagc tcttttttaa tgctttagca aaatatatca
     1921 gctaagacaa aagattaaat aactcaagcc atgcctataa gtcacttgtt tttaatccct
     1981 gtgtcagtgt caatctacgc cacagataac tgacagctct gagccctaga agtctaagct
     2041 tgataagttt actaaaaaca aaaataaggt gagccattac tatctgtttt tagatagtaa
     2101 atctttttaa gaatgactaa ctcatctagt catcacgtgt tttatttttc gattgttttc
     2161 atctcgattg ggtctgaaaa agagcgcttc tagtacttgg aaacgccctt ttgtatattc
     2221 tatcttcgta tgttgaatct aacaaccatt ggaagaatgg tctgacgttt cactcagtat
     2281 tttgacaggc tacccttcac caaaaatgca tgtgataaca aactccttaa aactctagtc
     2341 aaagggatct gactgttcct tgatcaaatg cttagctatt tttttgcgct ctcgcccttg
     2401 gcggaaaaag gtttgcatga tgttagcaca gtctgctgct agcaggcctc gttcaacttg
     2461 cacgcgatgg tttaagcgct catctgtcaa aatctggtaa aggctgtctg ctccaccaaa
     2521 tttttgattg ctggctccat aaataacatg cggaatcctt gctagcccaa tagcaccact
     2581 acacatcaca caaggctcga tggtcacaaa gagtgtggtg tctaataaac gccagttgcc
     2641 ttcatgagcg ttagcctcgt taatagccat catttcagcg tgcataatgg cttggttact
     2701 ctcttcacgc gcattgtgcc cacgaccaat aatctcacca tccttgacaa tgacacagcc
     2761 aatggggatt tcagctttct gtaaggattt ttcggcttct tttaaagcct cttgcatgaa
     2821 ataagtttgc tcttctaaac tatatggcat caagaaagac tccccaagtt ccaagatttg
     2881 tgtggtaagg caaggtcaac aggaatatta cctgcatagg cctgtgtttc ttgcctcaac
     2941 acttctaaat ggtttttgga attaatacct tctggaggaa ccggtggcat gtgggtcaag
     3001 acaagcttct taacacctgc ctggctagcc attagtcctg cttccttact ggtcaaatgg
     3061 gccatatgat tttcattgcc ttcgtataag taaacatccg ctaagaaaag gtctgcttct
     3121 tttgcgaagt ctgccaatcc ttcaaaataa ccactgtctc cagtaaagac aagtacctga
     3181 ccagtcgctc tttctacaat tcgaaaggca taacacacaa ctggatgaac tgttttaaga
     3241 aaggtaatgt caaaaggacc aatctgttca actccagtaa catcataggc tctaccttct
     3301 gaaacatcag gaatgcttaa cttagcaaat tcgtgtcggt cctcatcatg cccataaatg
     3361 ggcaatactt taggctgcca caggtgttta ggataaagtt gaaaataatg gcgcaacacg
     3421 cctaaatcgg ccacgtggtc tggatggtaa tggctaataa tcactgcatc caaatcgaga
     3481 ggactgattt ctttttccag ctcattgagc gctcgactac cagcatccat caataactga
     3541 aagccgtcat gtcctgtgac caaataagag gttgtgcctg catcttgata gggataggct
     3601 ccccaagacc ctaaggtggt taatttcatc tcaaaccctt ctttcttgct ttaactctta
     3661 ttataacaaa aattagtgtc aaagaggatt gagacccacc aaaaacaggt cctagcagat
     3721 aaatctgcca agacctgttc aacacaactt aactatttga tgattagatt actgttttgt
     3781 attcaaaaaa ctatctttgt atttaacagc gataatggtc gcaatgattg ctttgatggt
     3841 atctcctgga ataaagacca agtttgacaa aagcgatgtc acaagagaca tgccagtgta
     3901 ggctgccaac cagatagcac ccagaacatc aaccagtaca actcctgaga ttaaaagagc
     3961 taggaatgct aatggcgtgc tttttgagaa ccagttgcgg taaagtaatg aaaagacaat
     4021 aggcacaaga aggtaggcaa taacatagcc agcagatggc cccatcaata ctggaatggt
     4081 tgttcttgag cctgagaaaa caggtaagaa aaggccaatc actaaaaata agaaaacaga
     4141 taaggttccc tttttaccac ccagcatcaa tccagccaac atgacaccta agttttgtaa
     4201 aacaataggg actgggataa agcctagcgg gatagcagga ataaagccta aaataataat
     4261 caaagtggtc atcatagcca ccttaacaag ttctttggtt gtaaacatca gttacgactc
     4321 ctttaaaaaa tagatatcct tgtgcctatg acaggctgga ttaaagggac tttgacaata
     4381 aggacagcat ccacaaccat actcagctag gctcaagagc tttcgacaat gcccacaaat
     4441 aacaggagat gtttcttgat gtcctgttgg cacaaaggca tgatctgtta actggtcatg
     4501 gcaatggtaa caagcataat aagtctgaca aaaagcacat ttgagcccaa caatatcaag
     4561 cggggtatga taatgtaggc accgatactc ttggtcaagg tcaattccaa aacagtccgt
     4621 catatctcat cctcttgtaa tagtttaatc aaaagcttat gatttgtcaa ctctattttt
     4681 acaaaagggt aactgttttc tgaaaaagaa cacgtaaaac aagaactttc ctttaaaaag
     4741 tgacctatca gttgaaagca ataggacatc cgtctataca aaaaaacctg aaaccaaaga
     4801 cgatagatcg ccctcagttc caggatcata gcatttttaa ttagaaatta cggtttgatt
     4861 tattataacc ataccgttca agaaaatctt ggcggaattc aagtaagtta tcgtccataa
     4921 tagcctgacg tacttttttc atcaagttaa ccaagaaata aagattgtgg tagcttgtca
     4981 aacgaatacc aaaagtttcg tccgctttta gcaagtgacg aatgtaggca cggctatagt
     5041 tttgacaagt gtagcagtca cagtcatgat caagtggggt aaaatcttca gcgaatttgg
     5101 catttttaac cacaaggcgc ccctcactag tcatacaggt cccattacgg gcaatacgcg
     5161 ttggcaagac acagtcaaac atatctacac cacgaatcac accatcaatc aagctatctg
     5221 gtgctcccac tcccatcagg taacgaggtt tattttctgg taagagggga gtcgtaaagt
     5281 caaggacggc attcatttct tcatggctct ccccaacagc taaaccacca atagaataac
     5341 ctgggaaatc catagccacc aaatctgccg cagactgacg acgaaggtcc tcaaatccag
     5401 ctccttgcac aattccaaat aatccttggt catgaggacg acgatgggct tttaagccac
     5461 gttctgccca acggctagta cgttcaatgg attttttcac gtaatcataa ggttggtaaa
     5521 attgcgggca ctcgtcaaaa ctcatcatga tgtctgagcc aaggttattc tgaatggaaa
     5581 tggctttttc gggtgataga aacatttttg agccattcaa gtgatttttg aaagtcactc
     5641 cttcttcagt gatgtttcgt gaatcggcta aagagtaaac ttgaaaacca cccgaatcgg
     5701 ttaagattgg ctgatcccag ttcataaact tgtgcaaacc accagagcga gcaatcaact
     5761 catctcctgg acgcaaccag aggtgataag tgtttgataa aataatccct gatccaattg
     5821 cttttaactc ttctggagac tgtgttttaa cagtagcctg agttcccact ggcataaaca
     5881 ttggtgtcgg aaaggttccg tgaggggtga taatttcgcc cgagcgtgca ccggtgtgct
     5941 tttctgtttt gataaggcga tacttaatcg gataatctgt catgtgcttt cttccttgct
     6001 atcaagataa acagtcttga gcttttttga tctgcctgag tttccatcat aatgaagggc
     6061 ttcaagatga catgttgttc tagcgcctat cattttatca aaaaatccat aatttgtcat
     6121 gctttcccaa aaaagtatag tcaaaataat ggttagctgt ctcattttgt tttatggtat
     6181 aatgaaataa aggagtctgt tatgtcaatt aaagcaatca aaggccaggc tagagacacg
     6241 ttaaaaaatc tctctggcaa gtatttacta tttttaattc caacccttct ctttatgttt
     6301 catttcggta ttgagattca tcaaggttac gtcctatcta gtggtattga agtctcgcta
     6361 gcggctagtt atttcccatt actactagga cttatcctta gccttttcat tttatcagca
     6421 tcctttacaa tgattgatgt tgttaggcat tttcgtcaga aagtttcttt tgccgaatca
     6481 acaacagcct tctcaaaaga attctttggg aacctgctgg ttttagcgat cactaagtgg
     6541 ctattctttc tcatctggtc actaatttgg ttttttggcc tctttatttt cctttccgga
     6601 ctttcagcgt ttttggtcaa tgctaaatca ggtagctcaa cagtcatttc actgatcttt
     6661 ttactatttg gagcagtttt gtccttaatt ggttttggta tttatatcaa ccgttattac
     6721 gcttatagtc tatcagaata ccttctctac gataaggtta aagaggggac ttatctagga
     6781 gctatagccg tcattgaaac aagtgtagct atgatgaaag gttataaatg gaagcttttt
     6841 ttcctacaac ttagcttcac cggttggttt ctactgaata ttgttacttt tgggttactt
     6901 aatatctatc ttttaccata ttttacaaca gccaatgtca ttttttatga tcaactcaag
     6961 aaacgtttca aagacaaaga tgacccaatt gaaggagaac atttaagcct tcatcagctt
     7021 aataaaaagc ctactcctat gatgtatgac gatcaaaaag actaaataaa tcctccatcc
     7081 ataaaaatag cccttgaact gcaccccaaa agttagacac aaaatctaac gattggggtg
     7141 ctattttttt atgaaattta atcaagaaac gaaagttaag atttatgagt tacggcaaat
     7201 gggagagtcc attaaatcca tatcaaaaaa gtttgatatg gcagaatccg atctcaaata
     7261 tatgattcgc ttgattgata ggtatggggt aaccattgtt caaaaatgta agaataatta
     7321 ttattctcca gaactgaagc aagagataat aaatgaagtc ctgattgacg gccaatctca
     7381 gaaacaaaca tccttagatt atgctttacc aacttctagt atgctctcaa ggtggatagc
     7441 gcaatacaag aaaaacggtt atactattct tgaaaaacca agagggagac cgagtaagat
     7501 ggggcgtaaa cgtaagaaaa atttggaaga gatgacagag gttgaacgtc ttcagaagga
     7561 attagaatac cttagagcgg agaatgctgt gctaaaaaag ctgagagaat accgcttgag
     7621 ggacgaagta aagctcaaag agcaacagaa atcattcaag cattaaggaa tcaatttccc
     7681 ttagagatgc tacttgagat ccttgattta tcacgctcca cctattacta tcaagtgaag
     7741 cgactagctc aaggagataa ggatatagaa ctaaagcatg tgattcgaga gatttatgat
     7801 gagcataaag gtaattacgg ttatcgtcgt attcatatgg agttgcgtaa ccgaggattt
     7861 gtggtcaatc ataagaaagt tcaacgtctc atgaaagtca tgagcctagc agcccgcatt
     7921 cgtcggaagc gcaagtattc ttcttacaaa ggagaggttg gtaagaaggc tgataatctg
     7981 attaaacgtc acttcgaagg ctctaaacct tacgaaaaat gttacactga tgtgacggag
     8041 ttggccttac ctgaaggaaa actctactta ttacctgttc ttgacggcta taacagtgag
     8101 attattgatt tcactctgtc tcggtctcct aacttgaaac aggttcaaac catgcttgag
     8161 aagacttttc cagcggattc atacagcgga actattcttc atagtgacca ggggtggcaa
     8221 taccaacacc agtcctacca tgatttttta gagtctaaag gtattctgcc atccacgtcc
     8281 cgcaaaggga atagcccgga taacggcatg atggattcct tctttggcat tctcaaatct
     8341 gaaatgtttt atggactcga gacaacttat caatcacttg ataagcttga agaagctatt
     8401 acagattaca ttttttacta caacaacaaa cgaattaaag caaaactaaa aggatttagt
//
LOCUS       DATURV010000058         8432 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DATURV010000058 DATURV010000000
VERSION     DATURV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.058"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            67..2427
                     /locus_tag="VCF75_001359"
     CDS             67..2427
                     /locus_tag="VCF75_001359"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP2546912.1"
                     /translation="MEFVDKKLSEITPYKNNPRNNDEAVGPVAESIKEFGFKVPIVVD
                     KNGEIVNGHTRYKAAQKLGLETVPVIVADDLSEEQIKAFRLADNKVGEIADAYEVKAT
                     RERNGNYSLYIKYPLDGVFASVFKEEMKIKSDAGRRTKWQTFEINRVLRNSKDHIEIF
                     ARHISMRTQDIALKPFVNGASVGAESALEIWKKNLVGDDKFDVKSDILTLGSFNWEID
                     KIGNARGALGGVAGSILDVYGGEYEFDNRTIILRKQMGRKAPTVLEYGRNIVSVEEER
                     LLDGNYTSIYPYVRYTPQPKPQEEPPGKPHVGEHKQPEEQLVTLPEFILDGQYLSLYA
                     QRRIQMVDLSSHFNDDKNKKEPTIEEIRKLAQKYLKDNNVGAPKVSIEVDYIDLSQTL
                     DYQDFRVMEEVELCDIVPLYYPKFGITTESEKVVEIVYDVYTDSNHTIKLGTIGQSIS
                     KSLTGGVSERINALENNQKVITNSQKQFELNLPKYLNDLNGKRVWYEKPDDNIEHKIG
                     DYWFEKNGKYQRTWIWDGNQWVKVLDTEDLNPNQRAFDAAIAELEKAKKAQEEINQRT
                     DKELEEFRATLKNLALPEEAIKKITEAIKVDDIPSIKQSFDDLKNRVSETSEESRLTA
                     EILGNNGKTRYNKNLLVGEPNRTKSYDQDYIEVEANDGGFKRGETYTISFSQTCEPLK
                     KVAVTLTQANNKGVKLVLTPTKAKMEAQTFEVAKDKQSIEVYPLSYTAVLTGDWYKSK
                     QIDLNASEVRELALEMDYKEIADAKGATITGAWSDSPQIILDGGKK"
     gene            2424..3446
                     /gene="hylP"
                     /locus_tag="VCF75_001360"
     CDS             2424..3446
                     /gene="hylP"
                     /locus_tag="VCF75_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase HylP"
                     /protein_id="HEP2546913.1"
                     /translation="MSENIPLRVQFKRMTASEWARSDVILLESEIGFETDTGFARAGD
                     GHNRFSDLGYISPLDYNLLTNKPNIDGLATKVETAQKLQQKADKETVYTKAESKQELD
                     KKLNLKGGVMTGQLKFKPAATVAYSSSTGGAVNIDLSSSRGAGVVVYSNNDTSDGPLM
                     SLRTGKETFNKSALFVDYSGKTNAVNIAMRQPSTPNFSSALNITSGNENGSAMQLRGS
                     ERALGTLKITHENPSLEANYDKDAAALSIDIVKKQKGGKGTAAQGIYINSTSGTTGKL
                     LRIRNLNDDKFYVKPDGGFYAKETSQIDGNLKLKDPIANDHAATKAYVDGEVEKLKAL
                     LTAKQM"
     gene            3459..5345
                     /locus_tag="VCF75_001361"
     CDS             3459..5345
                     /locus_tag="VCF75_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEP2546914.1"
                     /translation="MSRDPTLTLDESNLVIGKDGRVHYTFTASQDNQTVRLASNCLGT
                     AHFNQLMIERGDKATGYVAPVVVEGTGNPTGLFKDLKEISLELTDTANSQLWSKIKLT
                     NRGMLQEYYDGKIKTEIVNSARGVATRISDDTDKKLALINDTIDGIRREYRDADRKLS
                     ASYQAGIEGLKATMANDKIGLQAEIKASAQGLSQKYDDELRKLSAKITTTSSGTTEAY
                     ESKLAGLRAEFTRSNQGTRTELESQISGLRAVQQTTASQISQEIRNREGAVSRVQQDL
                     ASYQRRLQDAEDNYSSLTHTIRGLQSEVGSPTGKIQSRLTQLAGQIEQRVTRDGVMSI
                     ISGAGDSIKLAIQKAGGINAKMSGNEIISAINLNSYGVTIAGKHIALDGNTTVNGTFT
                     TKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAITDLLEGKV
                     IKARNGAMLIDLNSAKLDFNSNATINFNSRDNALVRKDGTHTAFVHFSNATPKGYTGS
                     ALYASIGITSSGDGVNSASSGRFAGMRCFRHATGYNHTAAVDQTEIYGDSVLIADDFN
                     INRGFKFRPDKTKKILDMNNLYDAVVALGRCWGHLRNAGWDTARSNFIAAVTRELSNY
                     IDII"
     gene            5357..5785
                     /locus_tag="VCF75_001362"
     CDS             5357..5785
                     /locus_tag="VCF75_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEP2546915.1"
                     /translation="MDLTLKNKDLNTFYSVLDKIKITNMRANRGRAKLLAKVVDKINE
                     YAKDEADIIDLYALKNKDGKFVIDEHKNIKIEDPKKIDELNDLLTELGNEDITIKGHE
                     YSKRFIDFLEYLAESEDEFTSEEIVLIDNILEQFEESKGE"
     gene            5788..6405
                     /locus_tag="VCF75_001363"
     CDS             5788..6405
                     /locus_tag="VCF75_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEP2546916.1"
                     /translation="MRNWKVTGKYPQYDSTGAVASTHIIITAEDGSVISQPIKQDLTS
                     TNDTEIIKAALEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQRMINLQTIHVLTSGGKVEPDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEPFTYEAQTLEELESEDKVTVIKYAELVKQD"
     gene            6416..6715
                     /locus_tag="VCF75_001364"
     CDS             6416..6715
                     /locus_tag="VCF75_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546917.1"
                     /translation="MHFDLLQIGAACTALLSILGVWGFIVNPFKKAMEANEFAMAQLK
                     DSIKELAYELKNLDRDREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            6712..6897
                     /locus_tag="VCF75_001365"
     CDS             6712..6897
                     /locus_tag="VCF75_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546918.1"
                     /translation="MNKWFKKVAIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCVLMNVSQIKEEE"
     gene            7010..8212
                     /locus_tag="VCF75_001366"
     CDS             7010..8212
                     /locus_tag="VCF75_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEP2546919.1"
                     /translation="MAFLDKIKSAVIAEWHTHKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRAYDSWDESIADHGQFLVDNPRYE
                     AVIGETDYKKACYAIKAAGYATASSYVELLIQLIEENDLQSWDREALKNNKEETMTTA
                     NEIVQYCVNLANSGMGVDKDGAHGTQCCDLPCFVAKNWFGVDLWGNAIDLLDSASAQG
                     WEVHRMPTEANPKAGATFVQSVPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYV
                     GAPARFNTRDFTGVIGWFYPPYQGDTVTQPVSTEPQTSDTIVETAKTGTFTLDVAEIN
                     IRRWPSLASEVVGIYKQGDTVSFDSEGYANGYYWISYVGGSGMRNYLGIGQTDKDGNR
                     ISLWGKLN"
     gene            complement(8280..>8432)
                     /locus_tag="VCF75_001367"
     CDS             complement(8280..>8432)
                     /locus_tag="VCF75_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2546920.1"
                     /translation="EIGTKDGKHEQIDLFDSPNEGTRSDIFAKYKDNRIINMKNFSHF
                     DIYLEK"
BASE COUNT         2735 a         1620 c         1967 g         2110 t
ORIGIN      
        1 atctatggtt aaccaattag tcatcacatt gtggtgactt tttattatgt aaaaaaggaa
       61 taaaacatgg agtttgtgga caaaaaatta agtgaaataa caccttataa aaataaccct
      121 agaaacaatg atgaggctgt tggaccagtt gctgaatcta ttaaagagtt tggttttaaa
      181 gtgcctattg tcgttgataa aaacggcgaa atcgtaaacg gccacactcg ttataaagca
      241 gcccaaaaat tagggctaga aacagttcct gttattgtcg cagatgactt atctgaagag
      301 caaattaaag cgttcagact tgctgacaat aaagttggtg agattgcgga tgcttatgag
      361 gttaaagcca ctcgtgagcg caatggtaat tactcactgt acatcaaata tccgctagat
      421 ggtgtctttg cctcagtttt taaagaggaa atgaagatta agtctgacgc tggtcgtaga
      481 accaaatggc agacttttga gattaatcgg gtactacgaa atagtaaaga ccacatcgag
      541 atttttgcgc gtcatatctc tatgcgcaca caggatattg ctttaaaacc gtttgtaaac
      601 ggtgcgagcg taggagccga atcagcttta gaaatctgga agaaaaacct tgtcggtgat
      661 gataagtttg acgttaaaag cgacatctta acgcttggta gctttaactg ggaaattgat
      721 aaaatcggca atgcccgtgg tgctctagga ggtgtcgctg gctctatcct agatgtttac
      781 ggtggtgagt acgagtttga caaccgtaca atcatcttac gcaagcaaat ggggcgtaaa
      841 gctcccacgg tattggagta tggccgtaat atcgtcagcg tagaggagga gcgattgcta
      901 gatggcaatt acacctctat ctatccttac gtaagatata cgccacaacc aaaaccgcaa
      961 gaggaacccc ctggtaagcc gcatgtaggc gagcataaac aacccgaaga acagctagtg
     1021 acattgcctg aatttatcct agatggtcag tatctcagct tatatgctca gcgcagaatc
     1081 caaatggttg atttatcaag tcattttaac gatgacaaaa ataaaaaaga gccaacgatc
     1141 gaagaaatcc gaaagctggc tcagaaatac cttaaggata ataacgttgg tgcaccaaaa
     1201 gtcagcattg aggttgatta tattgacttg tcacaaacgc ttgactatca agattttaga
     1261 gtcatggaag aggttgagct ttgcgacatt gtaccacttt attatccaaa gtttggcatc
     1321 acaactgagt ctgaaaaagt cgttgagatt gtctatgacg tctatacaga tagcaaccat
     1381 acaataaaac ttggcacgat tggtcagtcc atctctaaga gtttgacagg tggggtctca
     1441 gagcgtatca atgctttgga aaataaccaa aaggttatca ccaacagcca gaagcagttt
     1501 gaactcaatc tgcctaaata cctcaatgac ctcaatggta aacgcgtttg gtacgaaaaa
     1561 ccagatgaca atattgagca caagataggc gactattggt ttgaaaaaaa tggcaagtat
     1621 cagcgtactt ggatttggga tggcaatcaa tgggtcaagg tactagatac agaggattta
     1681 aaccccaatc aacgagcttt tgatgccgca atagccgaac tcgaaaaagc aaaaaaagcg
     1741 caagaagaaa tcaaccagcg tactgacaaa gagcttgaag aatttagagc caccctcaaa
     1801 aacctagctt taccagaaga agcgattaaa aaaatcacag aggctatcaa agttgatgac
     1861 atcccgtcta ttaaacaaag ctttgatgac ctcaaaaata gagtgagtga gacaagcgaa
     1921 gaatctcgtt taactgccga aattttagga aacaacggta aaacccgcta caacaaaaat
     1981 ttattggttg gtgagcctaa tcgcacgaag tcttacgacc aggattacat cgaggtagaa
     2041 gccaacgacg gtggtttcaa gcgtggcgag acctatacga ttagctttag ccagacatgt
     2101 gagccactca aaaaagtggc tgtcacgctg acacaggcta acaacaaggg agttaagtta
     2161 gtgctgacac ctaccaaggc taagatggaa gcacagacgt ttgaggtcgc taaggataaa
     2221 cagtctatag aggtttatcc tttgagctac acggctgttt taactggcga ctggtataaa
     2281 tctaagcaga tagatttaaa cgcgtcggag gtgagggaat tggctctgga gatggattat
     2341 aaagagattg cagatgccaa aggtgcaact atcacagggg catggtcaga cagtccacaa
     2401 attatattag acggaggtaa aaaatgagtg aaaatatacc gctgcgagtc caatttaagc
     2461 ggatgactgc tagcgagtgg gctcgtagtg atgtcatctt actggagagt gagataggct
     2521 ttgagacaga cacaggtttt gccagagcag gtgatggcca caatcgattt agtgaccttg
     2581 gatacattag cccactcgat tacaatctac tgactaacaa gccaaatatc gatggattag
     2641 cgacaaaagt cgagaccgct cagaaactac aacaaaaagc agataaagag accgtctata
     2701 caaaagctga atcgaagcaa gagcttgaca agaaattaaa tctcaaaggt ggcgttatga
     2761 caggtcaact aaaatttaag ccagccgcca ctgttgctta ttcctcgtca acgggtgggg
     2821 cggtcaatat tgacttgtcg tctagcagag gtgctggtgt tgttgtctat tctaacaatg
     2881 ataccagtga tgggccgcta atgagcttgc ggacgggtaa agagaccttc aataaatcgg
     2941 cgctttttgt cgattacagc ggtaagacta atgccgttaa tattgcgatg cgccagccaa
     3001 gcacacctaa tttttcctct gcgcttaata ttactagcgg caatgaaaat ggtagtgcga
     3061 tgcagctacg agggtcagaa agagcgctag gaacgctaaa aattactcat gagaacccaa
     3121 gtcttgaagc gaattatgat aaagatgcgg cagcgttatc tattgatatc gttaaaaaac
     3181 agaaaggcgg aaaaggtact gctgctcaag gaatctacat taactcaaca tcaggcacga
     3241 cagggaagtt gcttaggatt agaaacctta atgatgataa gttctacgtc aagcctgacg
     3301 gtggttttta tgccaaggaa acttcgcaga ttgatggcaa cctgaaactc aaggatccca
     3361 tagcgaatga tcatgcggca accaaagctt atgttgatgg tgaagtcgaa aaattaaaag
     3421 cactcttaac ggctaagcaa atgtaaaaag gaggagatat gagtagagat ccaacgttga
     3481 cattagacga gtcaaatctc gttattggta aggatggacg tgtgcattat acatttactg
     3541 ctagtcaaga caaccaaaca gttagactag ccagcaactg cttaggcaca gcgcacttta
     3601 atcagctcat gattgagcga ggggataaag cgactggcta tgtggcgcct gtggttgtcg
     3661 aggggacagg taatccgact ggactattta aagacctcaa agagattagc ttagagctga
     3721 cagatactgc taattcccag ctttggtcaa aaatcaagct gactaaccgt ggtatgttgc
     3781 aggaatacta cgacggtaag atcaagaccg agatagtcaa ctccgccaga ggtgtcgcta
     3841 cacgtatcag cgatgatact gataaaaagc tagcgctcat caatgacacc attgatggta
     3901 tcaggcgtga gtatcgagat gctgatagga agctatccgc aagctatcag gcaggcatcg
     3961 aggggctaaa agccacaatg gccaatgata aaatcggttt acaagctgag attaaagcct
     4021 cagcacaagg gctatcgcaa aagtatgatg atgagttgcg caagctatcg gctaagatca
     4081 caacaacctc aagcggcact acagaggcct acgagagtaa gcttgcgggc ttacgtgctg
     4141 agtttactcg ctcaaatcaa ggcacgagga cagagctcga gtcacaaatt agcggactaa
     4201 gagcggtaca acagacaacc gctagccaaa tctcacaaga gatacgtaac cgcgaaggtg
     4261 ctgtcagccg tgtgcaacag gacttagcta gctatcagcg gcgattgcag gacgcagagg
     4321 ataattacag tagcttaacc catacaatta gaggtttgca gagtgaagtg ggatccccga
     4381 ctggtaaaat ccaatcacgc cttactcaac tagcaggaca aattgagcag cgggttacta
     4441 gagatggtgt catgagtatt attagtggcg ctggagacag cattaaatta gctatccaaa
     4501 aggctggcgg cattaatgcc aaaatgtctg gtaatgagat tatctcagca attaacctca
     4561 actcctacgg agtaacaatc gcaggtaaac acatcgctct cgatggcaat acgacggtta
     4621 atggcacctt taccacaaaa atagccgagg ctatcaagat tagggctgat cagattattg
     4681 caggcacgat tgacgctgct aggattagag tgattaacct taacgcaagt agtatcgttg
     4741 gtttagacgc taactttatc aaagctaaaa ttggctatgc tatcactgat ttgctcgagg
     4801 gcaaagtcat caaagctcgt aatggcgcta tgcttatcga ccttaacagc gctaagcttg
     4861 attttaacag taacgcgacc atcaacttta acagccgaga taacgctcta gtgcgtaaag
     4921 acggtacaca cactgccttt gtacacttta gtaatgccac accaaaaggt tatacaggtt
     4981 cggcactata tgcctctatt ggtatcacct catctggtga tggggtcaac agtgcgtcat
     5041 ctggacgttt tgcgggtatg cgttgcttta ggcacgctac aggctacaac cacactgcgg
     5101 cagtcgacca gacagaaatt tatggtgata gtgtacttat tgcagatgac tttaacatca
     5161 atagaggctt taagtttaga ccagacaaga cgaaaaaaat ccttgatatg aacaacttgt
     5221 atgatgctgt tgttgcactc ggacgttgct ggggacatct gaggaatgcc gggtgggata
     5281 ccgctcgcag caattttata gctgctgtca ctagagagtt gagtaattac attgatatta
     5341 tttaaaagga gacaacatgg atttaacact aaaaaataaa gacttaaaca cgttttatag
     5401 cgtacttgac aaaatcaaaa tcactaatat gcgtgccaac cgtggacgtg ccaagctact
     5461 ggcaaaagtg gtcgataaaa tcaacgagta cgccaaggat gaggctgaca ttattgactt
     5521 gtacgctcta aaaaataaag acggcaagtt tgtcatcgac gagcataaaa atatcaagat
     5581 tgaggaccca aaaaaaatcg acgagctcaa cgacttactg acagagcttg gtaatgagga
     5641 catcactatc aaaggccatg agtattcaaa acgctttatc gatttcttgg aatatctagc
     5701 cgaatcagaa gatgaattta catcagagga aatcgtctta attgataata ttttggaaca
     5761 atttgaagaa agtaaaggag aataattatg cgcaattgga aagtaacagg gaaatatcca
     5821 caatatgaca gcacaggagc agtcgcaagc acacatatta ttatcactgc tgaggatggc
     5881 tcagtcatct ctcaaccaat caagcaggac ttaacctcaa ctaatgacac agagattatc
     5941 aaagccgctt tggaagaatt taaaaaatct gaatacgttg aaattgctat gggcgaagca
     6001 gtccaaaaag ttgatgactt ggaaaaaatc tcacaagaaa ccgctaagac tgctaaaaca
     6061 gcccaaacag ccgctggact agctaaggtg tccgcagagc gtacacagcg gatgattaac
     6121 ttgcaaacca tccacgtatt gacaagtggt gggaaagttg aacctgatat ctacaaaggt
     6181 atgttagagc taatcgagcc agccaaaaaa ggtgagtatc aagcttatga cgtgtttacg
     6241 gtggttgaca gtactaaaga ggaagatggt gaagcaggcg aagggaactt agtctttgta
     6301 catgttaacg agccatttac ttatgaggca cagaccttag aggagcttga gtcagaggac
     6361 aaagtaacag tcatcaaata tgcggaatta gttaaacaag attaggggtg gttagatgca
     6421 ctttgattta ttgcagatag gagctgcgtg taccgctctc ttgtctatct tgggtgtttg
     6481 gggatttatc gtcaacccct ttaaaaaagc catggaagct aatgagtttg ccatggctca
     6541 gctcaaggac tcaattaagg agttagctta tgagcttaaa aaccttgatc gtgaccgtga
     6601 gattaccaaa aaaattatcg atcgccacga agagcgttta gggcgtgtcg aagacgaagt
     6661 tattatcaac aaagaacgta ttattacgct atttaaaaaa ggagaagaaa aatgaataaa
     6721 tggtttaaaa aagtagcaat caaaacaatc aaaacaatgg cacaaacagc tgttggtctt
     6781 atcgggtcaa gcgtgttgat tacggatatt aactggccaa cgatgttgtc agcggtatta
     6841 ctatcaggac taacttgtgt cctgatgaat gtgtcacaaa ttaaagaaga ggagtaacca
     6901 tgagaaaagc aatcacacaa ctagccgtca tcatagctat catagtgcta tattttccac
     6961 tggccgtgat tgctttgatt ttggctccat ttataggaga ggatgataga tggcattttt
     7021 agacaaaata aaaagcgcag ttatcgcaga gtggcacact cataagattt tgccatctct
     7081 gacagctgct caagccattt tagagagcgg gtggggcaaa catgccccac acaacgctct
     7141 gtttggtatt aaggcagata gctcttggac tggtaaatca tttgatacca aaacccaaga
     7201 ggaatatcaa gcaggtgttg tcacggatat tgtggaccga tttagggcgt atgatagttg
     7261 ggatgagtcg atagctgatc acggacaatt tttagttgat aatccacgct atgaggcagt
     7321 tattggggag actgactata aaaaggcttg ttacgctatt aaagcagctg gatacgctac
     7381 ggcaagtagc tatgtcgaac ttttaatcca actgattgag gaaaacgact tacaaagttg
     7441 ggatagagaa gctcttaaaa ataataagga ggaaacgatg acaaccgcaa acgaaattgt
     7501 acaatactgt gttaaccttg ctaattcagg catgggtgtt gacaaagacg gtgctcacgg
     7561 gacgcaatgc tgtgacttgc cttgttttgt cgctaaaaat tggtttggtg ttgatctttg
     7621 gggcaatgcg attgatttat tagacagcgc aagtgcgcaa ggctgggaag tccatcgtat
     7681 gccaacagag gcaaacccaa aagcaggcgc tacatttgtc caatcagtgc cgtatcatca
     7741 atttggacat acgggaattg ttatcgagga tagtgacggt tacaccatgc gcactgtcga
     7801 gcaaaacatt gatggcaatc ctgatgcttt gtatgtcggt gcaccagctc gttttaacac
     7861 tcgtgacttt actggcgtga taggttggtt ttacccacca tatcaagggg atacagtcac
     7921 gcaaccagtc agcaccgagc cgcaaacttc tgacactatc gtagagacag caaaaacagg
     7981 cacctttacc cttgatgttg cagagatcaa tatcagacgc tggccaagtc tagccagcga
     8041 ggttgtaggt atctacaagc aaggtgatac tgtcagcttt gatagcgagg gctacgctaa
     8101 tggctattat tggattagct atgttggagg ctcaggtatg cgtaactacc taggtattgg
     8161 acagactgat aaagatggga atcgcatcag cctttggggt aaattaaatt aggaggtaaa
     8221 gctccgagat aagacaaaac cgctcagatt attctgggcg gttttttgtg tatgatgaat
     8281 tatttttcaa gataaatatc gaaatgacta aagttcttca tattgataat tctattatct
     8341 ttatattttg caaaaatatc tgatctagtc ccttcatttg gtgagtcaaa taagtctatt
     8401 tgctcatgtt tcccatcttt tgtgccaatt tc
//
LOCUS       DATURV010000059         8324 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DATURV010000059 DATURV010000000
VERSION     DATURV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.059"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            248..946
                     /locus_tag="VCF75_001368"
     CDS             248..946
                     /locus_tag="VCF75_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2546921.1"
                     /translation="MFSGHQLKTARLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
     gene            complement(1063..2625)
                     /gene="ffh"
                     /locus_tag="VCF75_001369"
     CDS             complement(1063..2625)
                     /gene="ffh"
                     /locus_tag="VCF75_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEP2546922.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQLFGKGFKGKIGQFTMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(2640..2981)
                     /locus_tag="VCF75_001370"
     CDS             complement(2640..2981)
                     /locus_tag="VCF75_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEP2546923.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            complement(3070..3768)
                     /locus_tag="VCF75_001371"
     CDS             complement(3070..3768)
                     /locus_tag="VCF75_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2546924.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            complement(3851..5284)
                     /locus_tag="VCF75_001372"
     CDS             complement(3851..5284)
                     /locus_tag="VCF75_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546925.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            5451..7013
                     /gene="guaA"
                     /locus_tag="VCF75_001373"
     CDS             5451..7013
                     /gene="guaA"
                     /locus_tag="VCF75_001373"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEP2546926.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            complement(7055..8278)
                     /locus_tag="VCF75_001374"
     CDS             complement(7055..8278)
                     /locus_tag="VCF75_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP2546927.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLILINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
BASE COUNT         2482 a         1581 c         1484 g         2777 t
ORIGIN      
        1 gtaacttcag gcacagaatt tgtttagctc agaactaaat gataatttta tctttaaagt
       61 aattaactct ttatcagata tagacctgct agtacacgaa tctagtcgct atgttatgtt
      121 ttaggtgttc cccctttttg acagtgttac tatcacatga attaattgta gaaaatgtta
      181 cacttgcttt cgttttattt ttttgatact attagtaaag ttataacttt actaaaggag
      241 ctaccaaatg ttttctggac atcaactaaa aacagcacga ttatcaaagg gaataactca
      301 atcagaattg ggaagattgt tgcatgtcaa taaaatgaca atatctaatt gggaaaaagg
      361 taagaatata ccaaatgaaa aacatttaaa tgccttattg catctattca atgtgacatc
      421 tgattatttc gacccaaact atagattgct aacgccttat aaccagctga caatatctaa
      481 taaagaaaaa gtaattggct attcagagcg attgttaaat catcaaatag acaaaaaatc
      541 taaagatctc atagataaac catcacaatt atatgcttat cgggtctatg aaagtttatc
      601 tgctggtact ggttactcct attttggtga tggtaacttt gatgttgtct tttacgatga
      661 acaattagaa tacgattttg cgtcttgggt ttttggagat tctatggagc caacttattt
      721 aaatggtgaa gttgttctta taaaacaaaa tagttttgat tacgatggag caatttatgc
      781 agtcgaatgg gatgggcaaa catatatcaa aaaggtattt cgtgaagatg agggattacg
      841 tctagtgtcc ttaaataaaa aatattctga taagtttgct ccctatagcg aagaacctcg
      901 cattattggc aaaattatcg ctaattttag gcccttagaa atttaagtta gattgataat
      961 gaactcatca tggtatgtaa cacggtcttc cgtaatttta ataatcttgt atacaagata
     1021 aatgaatttc acatcaaaaa gagacgaaaa ttttcgtctc ttttattttc gttttttctt
     1081 tgcttttttt agtttgttag cctgccgttt catggcctgt ttcatggtaa attggccaat
     1141 ttttccttta aaccctttac caaatagttg actcatatcc atatcaccac cgaggccaga
     1201 taaatcaggc ataccacctt gtcccatcat tccttctaag gaagacatat ctggcatacc
     1261 agcaggcatg tttttaggaa gattattggg attaataccc atatctttca tcattttaga
     1321 catatcgccc gacataaccc cttgcatcat gcttttggct tggttaaagt ctttgataaa
     1381 tttattaact tctacaaagc tgttcccgga accagctgca atacgacggc gacgactcgg
     1441 gtttagtaag tcaggatttt cacgttcagc gggtgtcatt gaagagacaa tagcacgttt
     1501 gcgagcaatt tggttttcgt caactttgat attggccaaa gctgggttac cagccattcc
     1561 tggaatcatt ttcaagaggt cttccatagg ccccatattt tgcacttgat ctaactgttc
     1621 aataaaatca ttgaaatcaa aggtgttttc acgcattttt tcagctaact ctaaggattt
     1681 tttctcatca tattcctggc tagctttttc aatgagggta agcaaatccc ccattcccaa
     1741 aatccgactg gacatgcggt ctgggtgaaa tgtttcaata tctgtgattt tttcaccaat
     1801 acctgtaaat ttaattggtt ttccagtgat ttcacggaca gatagcgccg cacctccacg
     1861 tgtatcacca tcaatctttg ttaacacaac ccctgtgatg ctcaactgat gattaaactc
     1921 gtaggctaca ttggcagctt cttgaccaat catgctatct acaaccaaaa gaatttcatt
     1981 tggttgtgct aaagccttga catcgcgcag ctcttccatc agtttttcat caatttgcaa
     2041 gcgacccgca gtatcaatca agacatagtc attgcggttt tcacgagctt gttccaaacc
     2101 ttttctgaca atatcgactg ctgagtggtc tgtccccata tcaaaaacag gtacattgat
     2161 ttgttgcccc agtgttttta actgatcaat ggccgctgga cggtaaatat cggcagcaat
     2221 catcagtggt cgcgcgtttt cttctttaat taatttatta gcgagttttc ctgcaaaagt
     2281 tgttttacca gccccttgca aaccgaccat catgataata gttggaattt tgggagattt
     2341 atcaatctct gcagtttctg agcctaaaat actcgttaat tcttcattaa caatcttaag
     2401 aatttgttgt gttggatcaa gagtatcaat aatctcatgg ccaatagcgc gctcacgtac
     2461 gcgcttaata aaggttttaa caactggtaa agcgacatcg gcttctaaaa gagctagacg
     2521 aatctctttt gtcacttctt gaacatctga ttctgataat ttctttttac cgcgaatgtg
     2581 tttaaacaca tcttgcaaac gttgggttaa gctttcaaaa gccatagtca aaactcctct
     2641 tactctctat tgtcaatact ggttagaatg gaaatttttt cttgcaaata ctcatcatga
     2701 gggtagtgag caatcatgtc gtcaaagatt tcacttcgaa caacataatc cgaatacata
     2761 tgaagtttca tctcataagt ctctaaaatt ttttccgtac gcttaatatt atcatagaca
     2821 gcctgacgac tgacaccaaa ttcatcagca atctcagcta agctataatc atcggcataa
     2881 tacagttcaa tataattcat ttgtttatct gttagcaaag ctgcataaaa ttcaaaaagg
     2941 gcattcatcc tgtttgtttt ttcaatttcc ataatgttca ttttatcata ctatgattgt
     3001 ttttaacagt agcaaattaa ttgccttatg ctgttcgtgt aaaaaaggaa gtcataaatg
     3061 acttctttac taattatttt ctaagtaaaa ttcaaaacgg tcaccgatat actgactgcg
     3121 gacatactca aaaggtttgc cgtctgtaaa gtaagacacc tgagttagag ccaaaatggc
     3181 atgtccttta gcaacttcca agtaggaggc tacacgctca cttgctagct tagcgtagat
     3241 tgtctgttta cttttaccaa tctcgtaacc atttgctatc aatgagtgaa aaaagtgttc
     3301 tgtaatatca gctcgtttaa ccgtttttat aaatttttct gggattgaag caatttcgta
     3361 caccaaaggg acactgtctg catagcggat acgttccatt cgaatgacca agtctgtttt
     3421 gtctaagttc aattctttaa cttcggtatc actagctaat tgcttttgat aggataataa
     3481 tttagaggag ggttgtcgtc cttgggagcg tataatttct gtgaaacttg ttgttcctcg
     3541 cattttttct tgaacacgat gactggcaac ataagtacca cttcctatcc gcctttcaag
     3601 gatcccttct tctactagta atgtaatagc ctgccgtaga gtcattcgac taacggtgaa
     3661 atgttccgct aaatgtcgtt cacttggtaa gcgactacca attggccaga tgcccagatc
     3721 aatgtctttt ttaattgcat catgaatttt tatataagct ggtaacatgc cacactcctc
     3781 aagtcggtta ttcatctatt ttatcatatt tttgatgaga attgaattgt aaatattgag
     3841 gtatttggca tcaattaatg atattacctg cgacaataac tggtgaattt ggtggaaaat
     3901 gaataggttc cataaaccaa ctagtgagct tcgctttagt aacaatggat gaggtaaaaa
     3961 cattatttaa aaccctaata actcctacct ttgaggaatg ctgaccaata gaaccactat
     4021 aggctctcag gcaaccagtc ggatctatat aaggtaaaaa ttgattttgg cttacagtaa
     4081 tattgtgaca tagatgtcga gtttggttaa atgcttcata gtttggagca atattactaa
     4141 gtagacctcc atcccatgca aaatgatgaa cagcagcatc aaactgaatg acttctgaaa
     4201 aaaaatcatg cccttgagcc tttgatagca gctgttggtc ttgtactaat tctggcgcat
     4261 agccaataaa ttggtttttc tcaaacaatg aattttgtag agatcctaaa tcaaaaatat
     4321 gactattacg cttatgaacc atagtaaatt tgttatcgta aatatgccaa tcagtaccat
     4381 gatcagccat aatcataaag tggtctcctt ttttcaaatc atttgctttg aaatgcactc
     4441 cagtcaacac gagattacga acaccttgtt cagcctttgg tcctgtaggg aatccaaacc
     4501 acaacatttt accgtgaata atgaactctg tctgatgacc aataatagct gtatcagatg
     4561 gaagctcaaa ataatctttt tgtgaaggca aagagccaat gtgaaattgc ccctttggaa
     4621 aagatataac gctatttgga tgctgctttg cataagcgaa agcttttcgc aaaagacgat
     4681 tgttttgctc aggggaattt cctttattga acctaatata ataaggttta acaaaatctt
     4741 gctctgaaat tccatctaat cttagattat ctttcaggtt aatatcgtac tgatatcctc
     4801 ctaacgaata gtcatagcgt tttttaactt gtttaatagt cgctaattga ccttgataca
     4861 cttttatatg tggcttttta gttaatgaag ataatttaac gatatctcct tttttaaacc
     4921 tcggtttatc aggctctttc aggttatgtt ctggcaaaga aacagtgact tttcctattg
     4981 gaaataccac ttcataatga taactcactc ggtaattttc ggctatatct tctacctgtt
     5041 tattaatagt tccaatccat tgatgatatg gaccactttt ttgtacttga atagcttgtc
     5101 caatgtgata gcgaggctgt tgatctttgc ttcgttccac tattccgaca gttgctaata
     5161 tattgtgaag tgttgcaaaa ctattggtcc acgcaaggta cgaaacaact aaactcaaca
     5221 ataatcctag gaaaattaat tcaacaggtc gaaataaacc caaaattctc ttttttctta
     5281 gcataggccc tccctgttgt cttaattccc tttcatagcc tctattgtaa cataacgatt
     5341 acgttttttt ccaacgagct agagaaattg taatgtcttt tcgatgaaaa tatgctacaa
     5401 taagttgaca actatcagcc tgacggttgg tatagaatga aaggattata atgactgaaa
     5461 tttcaatttt gaatgatgtt caaaaaatta tcgttcttga ttatggtagc cagtacaatc
     5521 agcttattgc tagacgtatt cgagagtttg gtgttttctc cgaactaaaa agccataaaa
     5581 tcaccgctca agaacttcgt gagatcaatc ccataggtat cgttttatca ggagggccta
     5641 actctgttta cgctgataac gcctttggca ttgaccctga aatctttgaa ctagggattc
     5701 cgattcttgg tatctgttac ggtatgcaat taatcaccca taaattaggt ggtaaagttg
     5761 ttcctgctgg acaagctggt aatcgtgaat acggtcagtc aacccttcat cttcgtgaaa
     5821 cgtcaaaatt attttcaggc acacctcaag aacaactcgt tttgatgagc catggtgatg
     5881 ctgttactga aattccagaa ggtttccacc ttgttggaga ctcaaatgac tgtccctatg
     5941 cagctattga aaatactgag aaaaaccttt acggtattca gttccaccca gaagtgagac
     6001 actctgttta tggaaatgac attcttaaaa actttgctat atcaatttgt ggcgcgcgtg
     6061 gtgattggtc aatggataat tttattgaca tggaaattgc taaaattcgt gaaactgtag
     6121 gcgatcgtaa agttcttcta ggtctttctg gtggagttga ttcttcagtt gttggtgttc
     6181 tacttcaaaa agctatcggt gaccaattaa cttgtatttt cgttgatcac ggtcttcttc
     6241 gtaaagacga gggcgatcaa gttatgggaa tgcttggggg caaatttggc ctaaatatta
     6301 tccgtgtgga tgcttcaaaa cgtttcttag accttcttgc agacgttgaa gatcctgaga
     6361 aaaaacgtaa aattattggt aatgaatttg tctatgtttt tgatgatgaa gccagcaaat
     6421 taaaaggtgt tgacttcctt gcccaaggaa cactttatac tgatatcatt gagtcaggaa
     6481 cagaaactgc tcaaaccatc aaatcacatc acaatgtggg tggtctcccc gaagacatgc
     6541 agtttgaatt gattgagccc ttaaacactc ttttcaaaga tgaagttcga gcgcttggaa
     6601 tcgctcttgg aatgcctgaa gaaattgttt ggcgccaacc atttccaggt cctggacttg
     6661 ctatccgtgt catgggagca attactgaag aaaaacttga aaccgttcgc gaatcagacg
     6721 ctatccttcg tgaagaaatt gctaaggctg gacttgatcg tgacgtgtgg caatacttta
     6781 cagttaacac aggtgtccgt tctgtaggcg tcatgggaga tggtcgtact tatgattata
     6841 ccatcgccat tcgtgctatt acgtctattg atggtatgac agctgacttt gctcaacttc
     6901 cttgggatgt cttgaaaaaa atctcaacac gtatcgtaaa tgaagttgac cacgttaacc
     6961 gtatcgtcta cgacatcaca agtaaaccac ccgcaacagt tgaatgggaa taacagaata
     7021 aacattaaaa gactcgctca ttttggcgag tcttttaatg tttattcttt aactgttttc
     7081 gtattttgta catggtatta gcaatatgat aaagtgggct gacagggaga ttaaattctc
     7141 ctataaattc ttctattgtc ggagcaaagt tggccttaaa tttggtaagt ccatcatcaa
     7201 gcgagccttc tactcctccc atgttagccc aaataatacc atcttgataa gcatcctgaa
     7261 aaactttagg atacagcaga tactgaggat aaaacttctt aaaatcatca ttcatcccag
     7321 cataaagcat ttccataaca tttccataag aaatagataa tatacctgct acaacaactt
     7381 cctcaggata ttggtctgta aagccttcaa attcagttat ataacgttct aaagaagctt
     7441 tttgatcttc taattttttt aaacgcttct tttgatgagc ttgggtgcga gtaatatctt
     7501 ggttaataag aattaattgc tggcggtatt gatctagttt ttgaggaata ttcacttttg
     7561 ctagatgtaa gtaggcctta tccccataag tagtcatcaa cttttgaaag taagcttcgt
     7621 tacgcaaaga aatattttta cgtttttctg ttaaggagac aatcttggaa aatttgtgaa
     7681 gttctgattg actggcacga tatgtttcta caccacgctg cttagcatct tttattaaac
     7741 gtctggtatg cttaggaaat tgcatctcaa ggttttcttg agtgtaaata ttagcttgga
     7801 aacgaggttg gatactatct gcaatctcca ttgttaaacc agtccaatga acaccagctt
     7861 cttggagatt cttaatagct gctaaagcta aaggtttttc ttcttcttcc tgtcccagtg
     7921 cgtattgttt taacaggata gctggatcat atttgataaa gagggccttt ttagttttac
     7981 cataatcctt tagtgttttc atggtaaatg ccaccaaatc acggtcaaga taatccatga
     8041 ctggccctct tggaatataa ataatgcttc ttccaagtgg caatagctta atcaaaagtg
     8101 ataaagaggc aacctgcttt tcctctttat agataccaat tcgctcattc tgccattgat
     8161 ttttgatttt tgcccaatca ctaccttgta aaacgctaat ttgctgatgt tctttaacaa
     8221 aagagtcatg ctcttcttct gaaataccga tttttgcata aaacgttaaa ttcgtcattt
     8281 attgtcccca tctattcgta atcttagcag atgtacttac taat
//
LOCUS       DATURV010000060         8106 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DATURV010000060 DATURV010000000
VERSION     DATURV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.060"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            16..195
                     /locus_tag="VCF75_001375"
     CDS             16..195
                     /locus_tag="VCF75_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEP2546928.1"
                     /translation="MLTYDEFKQAIDNGYITADTVMIVRKDGQIFDYVLPGEPVRLWE
                     VATEEKVEGVMRELW"
     gene            1025..1594
                     /locus_tag="VCF75_001376"
     CDS             1025..1594
                     /locus_tag="VCF75_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEP2546929.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVATFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            1595..3547
                     /gene="gyrB"
                     /locus_tag="VCF75_001377"
     CDS             1595..3547
                     /gene="gyrB"
                     /locus_tag="VCF75_001377"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEP2546930.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEIIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            3915..5639
                     /gene="ezrA"
                     /locus_tag="VCF75_001378"
     CDS             3915..5639
                     /gene="ezrA"
                     /locus_tag="VCF75_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEP2546931.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(5771..6229)
                     /locus_tag="VCF75_001379"
     CDS             complement(5771..6229)
                     /locus_tag="VCF75_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEP2546932.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQTPEKTKSFWKRFLKK"
     gene            6462..7769
                     /gene="eno"
                     /locus_tag="VCF75_001380"
     CDS             6462..7769
                     /gene="eno"
                     /locus_tag="VCF75_001380"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEP2546933.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
BASE COUNT         2565 a         1329 c         1711 g         2501 t
ORIGIN      
        1 ataaggaggt aatctatgct aacatacgac gagtttaagc aagcgattga caatggatat
       61 atcacagcag acacagttat gatcgtgcgt aaagacggac agatttttga ttatgtgtta
      121 cctggtgagc ctgtaagatt gtgggaagtt gcgacagagg agaaggtgga aggagtgatg
      181 agggaattgt ggtgaaaagg gtgcaaaaaa ggggcaaaac ggattaaaaa tgaaactatt
      241 ttaaactttt tactataata attacctttt aaatcaacac ttgaactatt gtgaaccatt
      301 gtgtacaata gtagtagatt ccggcagggg tcattattgt cactgaaaaa gcctttacat
      361 caaaggcttt tttgtattac tcaattaatg ttaggagcac aaaagaggca gttcgattat
      421 taaccaattc ctccctattt acagatgtat ggctataaat ggacaatctt aagttagggt
      481 ctgagtgact aaacctgtcc ctaaccactt ttggtggtaa tcctttttca gctaaaagcg
      541 aaatgtgcga gtgtttgaat aagtgagtat ggtaatcacc ataaattttt aggcgcttat
      601 tgatgtgtgc atttaaaatt ggtaaacctt ttgaatatgg aaagctgtta gcactctgct
      661 tgtgattaat ctatcgtatg ttaaaagtga catttaaatt aaatcatttt ttattatttc
      721 aaaaggattt atcatattgg aaaatagcgg tgcttctatt ggcattttac tattgacagg
      781 aatttccctg ccttttgtct cctaaagaag atcagctttg atttctaact tgattagagt
      841 gggcttggta ctgtttatga attatcagtg catactttct ggagatcaaa aaggaaagga
      901 gcagctaaag aggtgtatgt gttaccatta gaggaataga acatcgcaag actcgtttat
      961 tcagtcatta cgatacctgt tttatttggg aaataactat aatattaagt gaatttgtga
     1021 tataatgaaa ggtatgaatt atcaagatta tatatgggat ttgggaggta ccttactcga
     1081 taattacgag ctttcaaccc aagcttttgt ccaaacgcta gcttttttca gccttccagg
     1141 agatcacgat gctgtttacc aaaaattaaa agaatcaacc gctatcgctg tggcaacgtt
     1201 tgctcctaat gagccagaat ttttgcatgt gtataggcta agagaagcag acaaattggc
     1261 acaacccatt tggtgtttgg gagctaaaga aattttggaa aagatagcga cttcaggttc
     1321 gcgaaatttt ttaatttctc atcgagattg tcaggtgaac caacttttag agcaagctgg
     1381 gttgttaatc tattttacag aagtcgtgac agcttcaaat ggctttgctc ggaaaccaaa
     1441 tcctgaaagt ttgttttatt taaaagaaaa gtatgatatt aatagtggat tagtaattgg
     1501 agatcggcta attgataaac aagcaggaca agcagcgggt tttaatacct tgcttgttga
     1561 cggtagaaaa aacctattgg agatagtaac gtagatgatt gaagaaaata aacattttga
     1621 aaaaaaaatg caagaatacg atgccagtca aattcaggtt ctagaagggc tggaggctgt
     1681 ccgaatgcgt ccagggatgt atattggctc gacagctaaa gagggtttgc atcatttagt
     1741 ctgggaaatt gttgacaact caattgacga agcattagca ggttttgcct ctcatattaa
     1801 agtctttatt gaagcagata attccattac agtagtagac gatggccgtg gaattccagt
     1861 tgacatccaa gccaagacag gacgtcccgc cgttgaaaca gtttttacag tcttacacgc
     1921 aggtggtaaa tttggcggag gcggctataa ggtttctgga ggactacatg gtgtagggtc
     1981 atctgttgtt aatgctttat caacacaatt agatgtgcgt gtttataaaa acggccaaat
     2041 tcattaccaa gaatttaaac gcggggctgt tgtagcagat cttgagatca ttggaaccac
     2101 tgatgtgact ggaacaacgg tacactttac acccgatcca gaaattttta ctgaaacgac
     2161 tcagtttgat tacagtgttt tagcaaaacg tattcaagag ttagcctttt tgaatcgtgg
     2221 tttaaaaatt tccattacag ataagcgctc aggtatggaa caagaagaac atttccatta
     2281 tgaaggtgga attggttctt atgttgaatt tttaaatgat aaaaaagatg ttatctttga
     2341 aacgcctatc tatacagatg gtgaattaga aggtattgca gttgaagtag ccatgcaata
     2401 cacgactagc tatcaagaaa cagtcatgag ttttgctaat aatattcata ctcatgaagg
     2461 tggaacgcat gaacaaggct ttagagcggc tcttactcgg gtcatcaatg actacgctaa
     2521 gaaaaataaa attcttaaag aaaatgagga caatttgaca ggagaagatg ttcgtgaagg
     2581 tttgacggcg gtaatttctg ttaagcatcc aaatcctcaa tttgaaggtc aaaccaaaac
     2641 aaaattgggc aactcagaag tggttaagat cactaatcgt ctctttagtg aggcctttca
     2701 acgttttctt ttggaaaacc cacaagttgc tcgtaagatt gtggaaaaag ggattttggc
     2761 ttctaaagct agaattgcag ctaagcgagc ccgcgaagtc acccgcaaaa aatcaggctt
     2821 agaaatttca aacttacctg gaaaattagc agactgttcg tcaaatgatg ctaaccaaaa
     2881 cgaacttttc atcgtcgaag gagattcagc gggtgggtcg gccaaatcag gtcgtaaccg
     2941 agagtttcaa gctatcttgc ctattcgcgg taaaattttg aacgtggaaa aagcaactat
     3001 ggataagatt cttgccaacg aagaaattag aagtctcttt accgctatgg gtacaggttt
     3061 tggtgcagac tttgacgtgt caaaagctcg ctaccaaaag ctggttatca tgaccgatgc
     3121 cgatgtggat ggcgctcata ttagaacctt gcttttaact ttgatttacc gctttatgag
     3181 gcctgttcta gaagctggct atgtttacat cgcccagcca cctatttatg gtgttaaggt
     3241 cggtagtgag attaaagagt atattcagcc aggtattgat caagaagacc aattaaaaac
     3301 agctcttgaa aaatatagta ttggtcgttc aaaaccaact gttcaacgtt ataaaggtct
     3361 tggggaaatg gatgaccatc aactttggga aactactatg gatcctgaaa atcgtttgat
     3421 ggcgcgtgtg acagttgatg atgccgcaga agcagataaa gtatttgata tgttaatggg
     3481 agatcgtgtt gaaccaagac gtgatttcat tgaggaaaat gcggtttata gtacactgga
     3541 tatttagtca cctttttagt ttcacaagga atctgctacc aaagaggacg aattgactaa
     3601 aaatcatgat gcatgatgcc aacagctttg ttagttagta cgtcttcatc ttgagggaca
     3661 gcgttaatgc ataggtattg agaccgcccc tttataccta tgttagtcct tattaagaaa
     3721 gccgtgtgag tcactggtac gagattgttg aattagtcaa atatattttg aaatgcattt
     3781 taaagtatgt tataatcgta atgttatagt gtattttaaa taaaatttta ataaaaggcg
     3841 attgaggaat ttcagttttt agttattact ggaacaatgt tgataaatcg tgtctaaata
     3901 ataaggagat tcagatgtca agcggaatta tcttgctgat tgtggctata gtcctactag
     3961 tgattatcgc ctatctagtg ggtgtcatca tacgaaagcg taatgattcc ctaattacca
     4021 gtctagaaga acgaaaacag gccttgtttg ctttgcctgt taatgatgaa attgaagaag
     4081 taaagtcact tcatttgata ggacagagcc aaacctcttt tcgggaatgg aatcagaaat
     4141 gggtagactt aactgtaaat tcgtttgcag atattgaaaa tcatattttt gaagctgaaa
     4201 atttgaatga tacgtttaat tttattcgcg ccaagcatga gattaatagc gttgaaagtc
     4261 agcttaatct tgttgaagaa gatatcgctt ctattcgtga ggcacttaat atcttaaaag
     4321 agcaagaaga aaaaaatagt gctcgtgtca cccatgctct tgatttatat gaaaagctcc
     4381 aagcttctat ttctgagaat gaagataatt ttggttcaac tatgcctgaa attgacaaac
     4441 agatgaaaaa cattgaaact gaattttcac agtttgtcgc actcaactca tcaggtgatc
     4501 ctgtagaagc ttctgaagtt ctggatagag cagaagagca tactattgct ctagggcaga
     4561 ttacagaaca gattccagct attgtggcca agttggaaga tgattttcca gatcagttag
     4621 atgatttgga aacaggatat cgccgtttac ttgaagaaaa ctatcatttt ccagaaaaaa
     4681 acattgaggc acgttttcag gagattcgtg agtctattcg tgctaattca tcagagttag
     4741 ttacacttga cttggatcgt gctagagaag aaaacacgca tattcaagaa cgcattgatt
     4801 ctctttatga agtctttgag cgcgaaattg ccgcttataa ggtagctgct aaaaatagta
     4861 agatgctacc tcgttaccta gcacatgtga aacgtaataa tgaacaactc aaagatgaaa
     4921 ttgcacgttt atcacgtaaa tatattctaa gtgaaacgga aagtctgact gttaaggcat
     4981 ttgagaagga tattaaagag atcgaagaca gtacacttgc tgttgcagaa caatttggtt
     5041 tacaagaaaa accattttca gaactgcaag tgacttttga acgcagtata aagacgctta
     5101 ctaatgtcga gtcaggtcaa atggatgttt ttgcagcagt taaagatatt gaaaaaattg
     5161 aatcacaggc acgccataac cttgatgttt atgttactca gcttcatatg attaaacgtt
     5221 atatggaaaa acgtcacttg cctggaattc cacaagactt tcttagtgct ttctttacaa
     5281 cgagttcaca gttggaagct ttgatggatg agcttagtag aggacggatc aatattgagg
     5341 cagtgtcacg tttgtcggaa gttgcgacgg ttgctattgc taatctagaa gatttaacct
     5401 atcaagtggt tcaaaatgct accttgactg aacaattact acaatattct aatcgctatc
     5461 gttcttttga agcaggagtt caaagtagtt ttgaacatgc tttgagactt ttcgaagtag
     5521 aaaatgatta tcaggcttct tttgatgaaa tttcttatgc tcttgaaaca gttgaacccg
     5581 gggtaacaga tcggtttgtt aactcttatg aaaaaacacg agagcacatt cgtttttgat
     5641 acttgaaagt cgtgatattc tcacgacttt ttattagctt tttggaccaa tacgtgtttt
     5701 tgactaaaat cttcttttta atgatcaaaa gaagaagtag taacagtgtt ttttgctatt
     5761 gagaggatag ttattttttc aaaaatcttt tccaaaagga tttggttttt tcaggagttt
     5821 gtttgctaag ttgatcgttt tttgtctgct ttaagatgac ttctaaacgt gtttccttca
     5881 gatttattgc gtggtcggta tgaaggataa acgcaaaagg tgaagtcata tgcgtttctg
     5941 taataatagt aacaggtaaa tggttttctt ttgctaattt catatagagt atttgaagtt
     6001 gaggtgccag cttggaagag atttttaagt gtaaaggttg atagactaac tgaaggctcg
     6061 gtaattctct tttcaaatag tcttttactg ttttagtttg agcttcgtct aataaagcac
     6121 ttaaaacgac tcgttcagca tacgttccta gatagtagcg ttgctgctca ggatttaatc
     6181 ttctttctcc ccaggctcct tttaatagct tatcttctaa attagtcata gataccctcg
     6241 cttctttata ataataatag tttctttatt atatcgattt ctacaataaa ataccaattt
     6301 gaaaattttc ggtaaatgtg ttaaaaaaca agaaagaaag tgatttttct tgcaaaaaac
     6361 aaaggaagct tttcatgaga ttgcttttta agttgattaa aggcctagtt tgtggtatta
     6421 tgaacatgta aaatattaaa actcataagg agagtattac aatgtcaatt attactgatg
     6481 tatacgctcg cgaagtcctt gactcacgcg gtaacccaac acttgaagta gaagtttata
     6541 cagaatcagg tgcattcgga cgtggtatgg ttccttcagg agcttcaact ggtgaacatg
     6601 aagccgttga acttcgtgat ggtgacaaat ctcgttacct tggtctaggt actcaaaaag
     6661 cagttgataa cgttaataac attatcgctg aggcaatcat cggttacgat gttcgcgatc
     6721 aacaagctat cgaccgtgca atgatcgctc ttgacggtac tcctaacaaa ggtaaacttg
     6781 gtgctaatgc tattcttggt gtttctatcg ctgttgctcg tgcagctgct gactatcttg
     6841 aagtgccact ttacacttac cttggcggat tcaacactaa agttcttcca actcctatga
     6901 tgaacatcat caacggtgga tcacactcag atgccccaat tgcattccaa gagttcatga
     6961 tcatgccagt tggtgcacct actttcaaag aaggtcttcg ttggggtgct gaagttttcc
     7021 acgctcttaa gaaaattctt aaagaacgcg gacttgttac agctgtaggt gacgaaggtg
     7081 gatttgctcc taaatttgaa ggaactgaag acggtgtaga aactatcctt aaagctattg
     7141 aagcagctgg ttacgaagct ggtgaaaacg gcattatgat cggttttgac tgtgcatcat
     7201 cagaattcta cgacaaagaa cgtaaagttt acgactatac taaatttgaa ggtgaaggcg
     7261 ctgctgttcg tacatctgca gaacaagttg attaccttga agagttggtt aacaaatacc
     7321 caattattac tatcgaagat ggtatggatg aaaatgactg ggatggttgg aaagttctta
     7381 ctgaacgcct aggcaaacgt gttcaattgg ttggtgacga cttctttgtt acaaacactg
     7441 aataccttgc tcgtggtatc aaagaaaatg cagctaactc aatccttatc aaagttaacc
     7501 aaatcggtac tttgactgaa acgtttgaag ctatcgaaat ggctaaagaa gctggatata
     7561 ctgccgttgt atcacaccgt tcaggtgaaa ctgaagattc aacaatcgct gacatcgcag
     7621 ttgcaacaaa cgctggccaa atcaaaacag gttcattgtc acgtacagac cgtattgcta
     7681 aatacaacca attacttcgt atcgaagatc aacttggtga agttgctcaa tacaaaggta
     7741 tcaaatcatt ctataactta aaaaaatagg cggtaagaca gaaatcggtc attcgtagaa
     7801 tgcgatttcg tcgtcgcacc tccgtcaggc tgactaggtt ggtcacgaca aaaggagtga
     7861 acatccagtc agctacgacg caaaagttaa agaatagaaa gcttgggaaa atcccaagct
     7921 ttttgctttc taaatagtaa aaatttactg tgggctaatt tatcctgagt taatgattcg
     7981 tgagaaaaac ttaacaactt gttaaaaaac tttggttctg ataatacagg accaaagttt
     8041 ttttatttag taaaaacgtc gtggtatagg tttgtccagg tattacatat ttcaaagtag
     8101 tatcta
//
LOCUS       DATURV010000061         7963 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DATURV010000061 DATURV010000000
VERSION     DATURV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.061"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            137..2335
                     /gene="nrdD"
                     /locus_tag="VCF75_001381"
     CDS             137..2335
                     /gene="nrdD"
                     /locus_tag="VCF75_001381"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEP2546934.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            2517..2663
                     /locus_tag="VCF75_001382"
     CDS             2517..2663
                     /locus_tag="VCF75_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546935.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRLQELKARFLEKSK
                     KQDN"
     gene            2692..3627
                     /locus_tag="VCF75_001383"
     CDS             2692..3627
                     /locus_tag="VCF75_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP2546936.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGQAVLYSDVKELPKASLDGVFIHAATSAHAELASVFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKELSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIAASQLSYGELKVELPKD"
     gene            3636..4130
                     /locus_tag="VCF75_001384"
     CDS             3636..4130
                     /locus_tag="VCF75_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2546937.1"
                     /translation="MEIRRPTLEDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLASQGVPAIQYVAFDEKNQALGFLNLRLRLNASLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSCAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEE"
     gene            4145..4759
                     /gene="nrdG"
                     /locus_tag="VCF75_001385"
     CDS             4145..4759
                     /gene="nrdG"
                     /locus_tag="VCF75_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEP2546938.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(4933..5664)
                     /gene="yaaA"
                     /locus_tag="VCF75_001386"
     CDS             complement(4933..5664)
                     /gene="yaaA"
                     /locus_tag="VCF75_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEP2546939.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFHYHPDLSTDHEFVYIKKEA"
     gene            complement(5706..6563)
                     /locus_tag="VCF75_001387"
     CDS             complement(5706..6563)
                     /locus_tag="VCF75_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP2546940.1"
                     /translation="MTYPYNSTISLGTVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTAFLVPDRHSLGLVLNHFLTRGISLEGAAD
                     HGYSEAIYLSDPEDNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKNARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
     gene            6764..7897
                     /locus_tag="VCF75_001388"
     CDS             6764..7897
                     /locus_tag="VCF75_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element IS1548 family transposase"
                     /protein_id="HEP2546941.1"
                     /translation="MIDFIISIDDCAVELDSRQSWKIGYPLSTILFLVFVCQLAGIET
                     WKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVISLVNSDRLKELKVQFEQSLTSLD
                     AVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLR
                     TIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEE
                     LQENAQYYQTVEKSRGQIEVREYWVSSDIKWLCQNHPKWHKLRGIGMTRNTIDKDGQL
                     SQENRYFIFSFKPDVLTFANCVRGHWQIESMHWLLDVVYHEDHHQTLDKRAAFNLNLI
                     RKMCLYFLKVMVFPKKDLSYRRKQRYISVHLEDYLVQLFGERG"
BASE COUNT         2433 a         1311 c         1811 g         2408 t
ORIGIN      
        1 gataacgaca aaaagtttta ttgtttatca gaaaaagtaa tctaatatca atatattgtg
       61 ttcgagcaaa aaacttgaca caatatattg atttttttag ttgtcgtgat ataatagttc
      121 cgtaaggaga tttgatatgg taagtttaga agaagacaag gtgactgttc aacctgatat
      181 taaagtgatt aaacgagatg gtcgccttgt taattttgat agtacaaaaa tctatagtgc
      241 tttattaaaa gcaagcatga aagtaactcg gatgtcgcca cttgttgagg ctaaattaga
      301 ggctatttct gatcgcatta tagcagaaat tattgagcgt tttccaacta atatcaaaat
      361 ttatgaaatc caaaatattg tagagcataa gcttcttgca gctaatgaat atgctattgc
      421 aaaagaatac attaattatc gtactcagcg tgactttgca cgttcacaag caacagatat
      481 caatttttct attgataaat taattaataa agatcaaaca gttgttaatg aaaatgctaa
      541 caaagatagc gatgttttta atactcaacg agatttaact gctggaatcg tagggaaatc
      601 gattggttta aaaatgttac cttcgcatgt tgctaatgct catcaaaaag gagatatcca
      661 ttaccatgat ttggattaca gtccttatac accgatgacg aactgctgtt taattgactt
      721 taagggcatg ttagccaatg gctttaaaat tggtaatgct gaagtggaaa gtcccaagtc
      781 tattcaaact gcaacagctc agatctcaca gattattgcg aatgtagcat caagtcagta
      841 cggcggatgc acagctgatc gcattgacga gtttttagcc ccatatgcgg agcttaactt
      901 taaaaaacat atggctgatg ctaagaaatg gatcgttgag actaagagag aaagctatgc
      961 ttttgaaaag actcaaaaag atatttatga tgcgatgcag tctttggagt atgaaattaa
     1021 tacgctcttt acgtctaatg gtcaaacacc atttacttct ttaggatttg gtttggggac
     1081 gtcttggttt gaacgtgaga ttcaaaaagc tattttgacc attcggatta atggtcttgg
     1141 tagtgaacat cgcacggcta ttttccctaa attaattttc acggttaaac gtggcttgaa
     1201 tttagaacca gattcaccaa actatgatat taagactttg gctttagaat gtgcgactaa
     1261 gcggatgtac ccggatatgt tatcttatga taaaattatt gatttgacag gatctttcaa
     1321 atctccaatg ggatgccgct ctttccttca aggctggaaa gatgaaaatg ggcaagatgt
     1381 gacctcaggc cgtatgaatc ttggggttgt caccctcaat ttacctcgca ttgccatgga
     1441 atcaaatggc gatatggata agttttggga gctgtttaat gagaggatgc taattagtaa
     1501 ggatgcttta atttatcgtg tcgaacgtgt cacagaagca aaaccagcaa atgcccctat
     1561 tctttatcaa tatggtgctt ttggaaaacg tttggagaag acagggaatg taaatgatct
     1621 ctttaagaat cgtcgtgcaa cagtctctct tggctatatt ggtctttatg aagtggcatc
     1681 tgttttttat ggtggtcaat gggaaggtaa tccagatgct aaagctttta ccttgtcaat
     1741 tgtcaaggca atgaaacagg cctgtgagga ttggtcagat gaatatggtt atcatttctc
     1801 tgtttattcg actccatcag aaagtttgac agatcgcttt tgtcgtttag atacggaaaa
     1861 atttggcatt gtgacagata ttacggataa agaatactat acaaattctt ttcactatga
     1921 tgtgcgtaag agtccgacac cttttgaaaa attagatttt gaaaaagatt atccagaagc
     1981 aggtgcttca ggtggtttta tccactactg tgagtatcct gttttgcaac aaaatccaaa
     2041 ggccttggaa gcggtttggg actatgctta tgatcgtgtg gggtatttgg gaaccaatac
     2101 gcctattgat aaatgctata attgccaatt tgaaggcgat tttaccccaa cagaacgtgg
     2161 ttttacttgc ccaaactgtg gcaataatga ccctaaaaca gttgatgtgg tcaaacgtac
     2221 atgtggttac ttggggaatc ctcaggcccg cccaatggtt aacggtcgcc ataaggaaat
     2281 ctctgcgcgt gtaaaacata tgaatggttc tactataaaa tacccaggcc tgtaaactca
     2341 agctggggaa gagccctttt gatggaaata tgatatgctt aaaaggttgc ttcatgcaag
     2401 caatctttta ttgtttaatg gtttcttagc aattgtgaga taagaataac taatccaaaa
     2461 cgactaatta gctgttagca atcggactat aagaagaaag agataaggaa gaaataatgg
     2521 gaaaatatca attagactat aaaggaatgc agcaggtaga acgctttcat gagaagcatt
     2581 ctaaaaaaaa gacggataaa aaatcccgcc ttcaagaact taaggctcgg tttttagaga
     2641 agtcaaaaaa acaggataac taaagtatcc agtaaagaaa gaaggctgag tatgcttaat
     2701 attgggattg tgggactagg tgctatttca caaaaagcct atttaccata tatgagacag
     2761 ctgagtgaca ttacttggca tctatcaacg cggaatgcag cagtacgtca gcaggtcggt
     2821 cagttatttg gacaggctgt tctttacagt gatgtcaagg agttgccgaa ggcaagttta
     2881 gatggcgtct ttattcatgc agccacatcg gcccacgcag agttggctag tgtgttttta
     2941 aatcagggaa tcccagtctt tatggataaa cctatagctg acaattacct gatgacaaag
     3001 aacctctatg acttagccaa agaaaatcag acttttctga tggcaggatt taacaggcgt
     3061 tttacacctc gtgtcaagga attgtcaagt ttgtcaacta agcgtaaagt ggctgttgaa
     3121 aaaaatgact tgaaccggcc aggagacatg acttttaagc tctttgattt ctttatccat
     3181 cctttagata cagccttatt cttgacagaa gggaccttgc taaaagggca ttttcaatac
     3241 catctggaag caggcttact gagtcaagtt atggtaacct tgatgaccga aagcatgacg
     3301 actacagctt ctatgaattt acaatcaggg agtcgccgtg aggtaatgga agttcaacgg
     3361 gctgaagaaa cttatcattt agagaactta gacgagttat ctatttataa aggtactgaa
     3421 aaaagggtgc ttggctttgc ttcttgggac accaccctgc acaaaagagg ttttgaaaca
     3481 atgatcgatg cctttttaga agctattagc acaggcgtca atcctgtcag ccctgagtct
     3541 agtcttttaa gccactggat ttgtcaacag attgctgcct ctcagctttc ttatggagag
     3601 ttaaaggtag agttgcctaa ggattaggaa ttaatatgga aataagacga ccgactttgg
     3661 aagataaaga tgctgtattg tcaatgatta acgaattttt agagcagaaa agtgctacag
     3721 atgggttatg gcattttaac gtcaatgact ttaactatga aacatggttg gaagattcct
     3781 tgcgacagga aatgggtttg gccagtcaag gagtgcctgc tattcaatac gtcgcatttg
     3841 atgagaaaaa tcaagctttg ggatttttaa accttaggtt acgtttaaat gcttcattac
     3901 ttgaaaaagg tgggcatatt ggttactctg tccgtcccag ccagcgtggg aaaggttatg
     3961 ccaaggagat gcttaaacaa gctgtcagct gtgcgatttc caagaatatc acgacaattt
     4021 tggtaacttg tgatgagact aatgttgcaa gccgagcagt tattgtggct aacggcggtg
     4081 tcttggaaga tagtcgaggt ggaacggaac gttattggat tgaagaataa agaggcgata
     4141 agtgatggca gaaaagtgtt ggaataatcc taagccaaag gaatggcagg cagaggagtt
     4201 aagtcaaggc cggattattg actataaggc ttttaacttt gttgatggtg aaggggttcg
     4261 taattccctt tatgtgtctg gttgtttatt ccattgcaag gggtgttata atgctgcgac
     4321 ttggtctttt aaggcgggaa tgccttatac gcaagaactt gaagagcaga taatgacgga
     4381 tttggcacag ccttatgttc agggactgac acttttaggt ggagaaccct ttttgaatac
     4441 gggtatctta attcctctga ttaagcgtat tcggcgtgaa ttgccagaaa aagatatttg
     4501 gtcttggacg ggttatactt gggaagaaat gatgcttgaa acaccagaca aactggaaat
     4561 gttgtcctta attgatattc tggtagatgg tcgttttgat atcacaaaaa agaacctcat
     4621 gttgcaattt agaggctctt ctaaccagcg aattatcgat gttcaaaagt ctttggctgc
     4681 taaagaagtg attatctggg ataagttaaa cgatggggac caaacctttg aacaaattag
     4741 tcgggaggat ttgctttaag aagctgactt atccacatga tttttttgaa aaggtcatgt
     4801 gaaaacaggt cctttaaggg tggattgggg agaaagtaat tgttattccc tggttttccc
     4861 cacattatcc tcatagttat ccacaggttg tgtataagtt gtagaattgt gaataaagac
     4921 taaaaaagag gcctaggcct cttttttgat atagacaaat tcatgatctg ttgataggtc
     4981 tggatggtag tgaaaaccag caaagacaag gcttttaagg ctgtcaacag tttggcaatt
     5041 attttccata caggcagtga gaaatgcacc tcgtgccttt tttgaaatgg tggaatgggt
     5101 tttgaattgg ccctcttttt ctgccataaa cttgggactt atccacaatt gtttacagtc
     5161 tttggaaaag acatcgtcaa attcactgga taaaagggaa atcacttggg gatgctcttt
     5221 ggcaaactga ttataacagg gccgccagta agacttgagg ctttgtcctt caatttttat
     5281 tctggtgtga aaatcatggc ggtgctctgc gataggatga ttagcgggaa tgataccgta
     5341 aaaggatgag gtgatgtaaa cttgctgggt gaggtaagcc tgttcttggg tagtgagctt
     5401 atcgcgcttg atatggcggt acataaggcc attgaacagt tggtaggcag ggtaagccaa
     5461 gctttgttga gaggccatgt cttgccagcg ttgctgttct tttttagctg cctcttcttt
     5521 gatacgatag gatttggcca agtcctccgt ggtcatagct gccatgattt ttaaaatggc
     5581 ttggctgtct tgtggaagga ggtgtgggtg agattcctta gggatcatca tttcttttgc
     5641 ggttggaatt aaaaaggtta gcatagtgtt attgtagtaa agttacttgc tttctgtcaa
     5701 gaaaactagt cttgtccaag gatgacgttc acacagatac cttcctcgtc ttctgtcgtg
     5761 aaagaagaag aatcttcttg taaaatagct aggccgtgta atcttgcgtt tttcagagta
     5821 gctgaaaaaa gcaggggcgt ctcaatgtga atggtcaaga aggctaagcc tggggcacct
     5881 tcttggtgtt ttttgaggta aggggctgac cagtgattga atgcaaggtg atgatggtag
     5941 ttccctgaag caatccagct agcacttgga atggtcattt tatcgcctaa atcaaaaacc
     6001 ttttgataga gaagtgaaga agccagagca tttttgacac tcagatggac atggccaatg
     6061 cgtgtgtctt gagctagtaa aaagcgcttg gggatatctg tcaactgctc caatatgctt
     6121 ttggtatccg tgggctcagt cacaccaata atttgaccat tgtcgcggat atcccaatgc
     6181 tcaacagctt tgtcatgata aatttcaatc ccattgtcct caggatcgct gaggtagata
     6241 gcttcgctat agccgtgatc agctgctcct tctaaagaga tacctctggt taagaaatga
     6301 ttgagaacaa gtcccaagct atgacgatca ggaactaaaa aggctgtgtg ataaagacca
     6361 taagccttat ttgctggtaa aggtgtttgt cgtaactcta agataacggt tttaccgtct
     6421 gtagtcaatt gacgagatgt tgtgtcttgt gagagaacct gtaaaccgat aatacttgtg
     6481 taaaaggttg tcatctttgc tagatcggta acatttagag aaaccgttcc taaagagatg
     6541 gttgagttgt aagggtatgt catcctatta cctccaaaaa ctaattaaat ctatcataac
     6601 caatcagttt caaaaagaaa agtaggcaca aataagtggt ataggcacta aaaagtaact
     6661 catttatcca cgccaaacgc atgaagaaac ttttgttttc agaaaactag attcataatg
     6721 acaaaatcaa tcaaagtagt acactataga tgaggtgact acgatgattg attttattat
     6781 ttctattgat gattgcgcag ttgaattgga tagtcgtcaa tcttggaaaa ttggctaccc
     6841 cttatcaacc attctatttc ttgtcttcgt ttgtcagtta gctggcattg aaacctggaa
     6901 ggagatggaa gattttattg aaatgaatga accattgttt gcgacctacg ttgatttgag
     6961 tgaaggttgt ccgtctcatg ataccttaga gcgtgtgatt agtcttgtta attcagaccg
     7021 tttaaaagag cttaaagttc aatttgagca atcattgaca agcttagatg ccgttcatca
     7081 actgatttca gtggacggta aaacgattcg aggcaatcga ggtaaaaatc agaagcctgt
     7141 tcatattgta acggcttatg atgggggtca tcatcttagt ttgggacagg tagcggttga
     7201 ggagaaaagt aatgaaattg ttgccattcc tcagttattg cggacaattg atatccgtaa
     7261 aagcattgta acgatagacg caatgggcac gcagacggct atcgttgata cgattattaa
     7321 aggtaaagca gactattgct tagccgtcaa aggaaatcaa gaaacacttt atgatgatat
     7381 tgctctttat tttagtgatg tcaacttatt ggaagaactc caagaaaatg cacagtatta
     7441 tcagactgtt gaaaaatcta ggggacagat tgaagttaga gaatactggg tgtcttccga
     7501 tatcaaatgg ttgtgtcaaa accatcccaa atggcataag ttacgtggta ttgggatgac
     7561 tcgtaacacg attgataagg atggtcagct gagtcaagag aatcgttatt ttatctttag
     7621 ctttaagccg gatgtcctca catttgccaa ttgtgtacga ggtcattggc agatagagag
     7681 tatgcactgg ttattggatg ttgtttatca tgaagatcat catcagacat tggataaaag
     7741 agccgcattt aacctaaatc ttatccgaaa aatgtgctta tattttctca aagtgatggt
     7801 atttcctaaa aaagacctca gttatcgtcg caaacaacgg tatatttctg tccatttgga
     7861 agattattta gtccaattat ttggagaaag aggctaagca gatgattgat ttaaaaagga
     7921 atcttttcaa aattgatgtc cgggcacaaa aggatgaaga gaa
//
LOCUS       DATURV010000062         7618 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DATURV010000062 DATURV010000000
VERSION     DATURV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.062"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            15..1733
                     /locus_tag="VCF75_001389"
     CDS             15..1733
                     /locus_tag="VCF75_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP2546942.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENTALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(1951..2907)
                     /locus_tag="VCF75_001390"
     CDS             complement(1951..2907)
                     /locus_tag="VCF75_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546943.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(2909..3973)
                     /locus_tag="VCF75_001391"
     CDS             complement(2909..3973)
                     /locus_tag="VCF75_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546944.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            complement(3966..5498)
                     /locus_tag="VCF75_001392"
     CDS             complement(3966..5498)
                     /locus_tag="VCF75_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546945.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGVPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVLPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            complement(5637..6689)
                     /locus_tag="VCF75_001393"
     CDS             complement(5637..6689)
                     /locus_tag="VCF75_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP2546946.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            complement(6783..7172)
                     /locus_tag="VCF75_001394"
     CDS             complement(6783..7172)
                     /locus_tag="VCF75_001394"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEP2546947.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
BASE COUNT         2491 a         1582 c         1277 g         2268 t
ORIGIN      
        1 gaaggagtat ttcaatgact tacacagaaa attttcaaaa atggttagat tttgagcagc
       61 ttcctgacta tcttcgtcaa gaattactat caatggatga aaaaacaaaa gaagatgctt
      121 tttacacaaa cttagaattt ggtactgctg gtatgcgtgg ttatattggt gctggtacaa
      181 atcgtattaa tatttatgtt gtccgtcaag caaccgaagg attagcaaaa ttaatcgaaa
      241 ctaagggaga ggaagctaaa aaacgtggtg tagccatcgc ttatgattcg cgccacttct
      301 ctccagaatt tgcttttgag tctgcacagg tcttggcaca gcatgggatt aaatcttacg
      361 tatttgaagc tcttcgtcca acaccagaac tctcttttgc tgttcgccat ttaaatgcct
      421 atgctggtat tatggttact gcaagccaca atccagcacc tttcaatggt tataaagttt
      481 atggacaaga cggaggtcaa ctaccaccag ctgatgctga tgcactaaca gactttatcc
      541 gtgctattga aaatccattt gcagttgaac ttgctgattt agatgaaagc aaatcatcag
      601 gtcttattca agttatcggt gaagatgttg atatggaata tcttagagag gttaaagatg
      661 ttaatatcaa tcaagattta atcaataact ttggaaaaga catgaaaatt gtctatactc
      721 ctctccacgg aactggtgag atgttgacac gccgtgccct tgctcaagcc ggttttgaat
      781 ctgttgttgt agtcgaatca caagctaagg cagatccaga cttttctact gttaaatcgc
      841 ctaaccctga aagccaagct gcctttgctc ttgctgaaga actcggacga gaagttgatg
      901 cagatgtctt agtagctact gatcctgatg cagaccgcct aggcgttgaa attcgccaac
      961 cagatggttc ttataaaaac ctttctggaa atcaaattgg agcgattatt gcgaaataca
     1021 tcctggaagc tcataaaaca gcaggaacac ttccagaaaa tactgctctc gctaaatcaa
     1081 tcgtatcaac agaattagta acaaaaattg ccgaaagtta tggcgctaca atgtttaacg
     1141 ttttaactgg attcaaattc atcgctgaga aaattcaaga gtttgaagaa aaacataacc
     1201 acacctatat gtttggtttt gaagaaagct tcggatatct tattaaacca tttgtccgtg
     1261 ataaagatgc tattcaagca gttcttttgg ttgctgagat cgcagcttat taccgttcgc
     1321 gtggtttaac acttgcagat ggtattgatg aaatttacaa agaatatggc tattttgctg
     1381 aaaaaacgat ttcagtaaca ctttcaggtg tggatggagc tgctgaaatt aagaagatta
     1441 tggacaaatt ccgtgaaaat ggtccaaaac aatttaacaa tactgatatt gtattattag
     1501 aagatttcca aaaacaaacc gctactaaaa atgatggaac aatatctaac ttaacaactc
     1561 ctccaagtaa tgttttgaag tacacgttag ctgatgattc atggattgca gttcgccctt
     1621 caggaactga accaaaaatc aagttctata ttgccacaat aggtgatact cttgacatcg
     1681 ctcaagaaaa aattgctaac attgaaacag aaatcaatac ctttgttggc taaaacaact
     1741 taagagctat cagtaaaaac gaaagacaag caaaaagaac taagccgctg acttagttct
     1801 ttttagtcta ttcttgcttc tattgtgatg gtgactggca atccaaacgc ttgcggtaag
     1861 tcccaaaaaa gcacccttaa agctagacct aactatcttg ctttaggagt gctgttaaac
     1921 ctcactaaga ggcttactac taatataacg ttatttagat ttaatatagt tgattccatc
     1981 agcttttggt gcaactgctt gaccaaagaa agcagctaaa ataataatcg ttaccatata
     2041 aggtgcaatc tgtaagtaaa ccgttggaat cttttctagt aatggtaatt gagcaccaat
     2101 aacggctaaa ctttgcgata agccaaagaa aagactagat agcatggcgc caactggatt
     2161 ccatttacca aagatcatag ctgccaaagc aataaatcca ggacctaaga tagttgttac
     2221 agcaaagtta acggagattg actgagcata aacagctcca cctattccac caagaaaacc
     2281 tgagatcatc acaccatagt atttcatcaa atagacgtta ataccaagtg tgtcagctgc
     2341 ctgagggtgt tccccaactg atcgtaaacg cagaccaaac ctggttttat aaagcaaaaa
     2401 ccaagcaaag aacgaaaaag caatggcgaa gtaaccaata aggctagtat ttttaaagaa
     2461 aatatcacca atcacaggaa tttgtgataa acctgggaaa tcaaacttcc caaaagattg
     2521 ttgaatgttg tctgtttgtc ccttaccgta catagcttta accaagaaga cagcaaaaga
     2581 aggtgctaac aagttcaaca ctgtaccact gacaatgtgg tcagctcgaa agttgatggt
     2641 tgctacagcg tgaatcaaag aaaaaatcaa accaacaata ccaccgacta aaacggctat
     2701 ccaaggcgtt gcttttccaa atgtttcagc aaactctaag ttgaaaacaa tcccagagaa
     2761 cgctcccatg accatgatac cttctaaacc aacattaaca acgcctgagc gctctgagaa
     2821 agttcctcca atacttgtga aaatgagtgg tgttgcataa ataagcatag atgacaccaa
     2881 taaggcaaaa attgttacta aactcatctt atttaccacc tttcactaat tttttagggc
     2941 gaataatgta acgttcaatg aggtaatgtg aaccaacaaa gaaaatgatt gatgcagtta
     3001 ctactttaac aagttctggt ggaatgcctg caatattcat tcctggagca ccaatattta
     3061 agacaccaaa taggaatgat gagaaaaata ttccaagcgg tgaatttgct gctaataagc
     3121 taactgccat accgtcaaaa ccaactgcta aagaagatcc ttgtacaaaa acattttcaa
     3181 aggttcctag accttcaact actcctccaa gacctgcaag agctccagag ataatcattg
     3241 ataaaatgat agtacgtttt gatgacatcc ctgcgtattc tgaagcatgt gggttgagac
     3301 caactgaacg aatctcaaaa cctaaagtag ttttgttaag caagaaccag attagggcaa
     3361 tggcaataat ggcaaagaaa ataccaatat taatacgaga gttattagtt agggctgata
     3421 accaatgcgt ttgataacta gcattatcac taacttgaat agttgaatca atactttgtt
     3481 tgacactctc aggataacca cgttgaacaa tagcatttcc gacatataaa atgatgtagt
     3541 tcatcataat cgtaacgata acttcacttg taccaagata agcacgaagt atccctggaa
     3601 tagctccagc aataccacca gcaatcattc cgataagagc tgtcataaga atcaataatg
     3661 gacgaggcat atctggattt aagagtgcaa accaacctgc tgaaatccat ccagctaaag
     3721 cctgtccaga caaaccaacg ttaaagaagc ctgcacggct agcaacagta aagccaagcg
     3781 ctattaaaat caatggcccc attgaacgga atatttctcc aatattttta acactaccaa
     3841 aggcaatttg gaataaacct tcataccccc agataggatc ataaccaaag ataaccataa
     3901 taatagcgcc aagtaaaaat cctagcaaga cagaaatcaa tggtacagca attttttgcg
     3961 catttttaga catgaccttc ctccttatga attgaaccac ctgccattaa aatacccaat
     4021 tcttgtttat tggtattttc tggcaatacg ataccttgaa ttttaccatc atgaataaca
     4081 gcaattcgat cagataaatt caatatttca tcaagttcaa aactaacaac gaggacagct
     4141 ttacctttat cacgttcttt aatcaaacgc ttatggatat attcaatagc tcctacgtca
     4201 agaccacgtg ttggttggct aacaataagt aaatctggat cacggtccac ttcacgagca
     4261 ataatggcct tttgttggtt accaccagaa aatcctctag ctggtactag ctcattggct
     4321 ccgcgaacat caaattcttt cattaattga cgagcatagt cattaatttt agtataattt
     4381 aagattccat tttgacttag aggttgtttg taataggttt gtaaggctgt attttcagct
     4441 aaagaaagat ctagtatcaa accatctcga tgacgatctt ctggcacgtg acccactgat
     4501 aactcagtaa ttttgcgtga agacaaatga gtgacttcat tgtttttaat cataatactt
     4561 ccagatttga ctttacgtaa acctgtaata gcttgaatta actcagattg accatttccg
     4621 tcaattccag caatccctac aatttcacca gctcggacat ctagagatag ccccttaact
     4681 gctggaaccc ctctattttc atctacttca agatttttga tggataagac cacatctttt
     4741 ggttcagcag cttttttgct agttgtaaag gaaaccgagc gtcccaccat catctcagct
     4801 aaatcttgtg aagttgctcc tgccacatca actgtttcga tactcttacc acgacgaata
     4861 actgtgacac ggtcggcgac agcacgaatc tcgtccagtt tatgggtaat taaaataatt
     4921 gattttcctt ctttcacaag attcttcatg atagtcatca attctttaat ttctgcaggt
     4981 gttaaaacgg cagttggctc atcaaagata agaatatctg caccacgata caaggttttc
     5041 aaaatctcaa cacgttgttg tgctcctact gaaatatcag aaactttagc tgatggattg
     5101 attgcaaggc catatttttc ggatagtacc ttaatatctt tcgatgcttg gttaagatct
     5161 aagcacccat tttttactac ttcatttcct aaaataatat tctcagcaac cgtgaaggct
     5221 tctactaaca tgaaatgttg atgtaccatt ccgataccaa gcttagctga tttagatggc
     5281 gaatcaattt gaactggctt gtcattaatg acaatttctc cactagtagg ctctaagaga
     5341 ccagctagca tattcatcaa agttgattta cctgccccat tttcacccaa taaggcatga
     5401 atctcacctt ttctgacatt taagttaatg tggtcatttg cgacaaaatc accaaatttt
     5461 ttcgtgattt ctctcatttc aatgacatgc tgtgtcatag gatcatttcc tttcaatgca
     5521 atcttttatt tcagtaaagc ctatgatcta tcacctatca caaactttac tgagataaaa
     5581 attatctcag tttaaaaaga gcgaccgaca aaggtcgcgc ttcttgttct taaacactat
     5641 ttttcaggaa ctttaatgtc accagattta atttttgctt tcgcttcttt aatagcttta
     5701 acagcttctt ttgaaacatt tgtagttgcg atttcaacac cgccatcttt taaaccatag
     5761 acagttgttt ttcctccagg gaatttttta tctgctactt gtttgttgat taactgaaca
     5821 gctttaccaa cttctttgat tgatgatgca agtacaaagt ttgcttcttt gccatcttta
     5881 gaagtgtatt ttccttcgtc tttttgatca cggtcaacac caataaccca aactttatca
     5941 gcttcactac gtttttcatt aatagctttt gcttcattaa atacacctgc tccagtgcct
     6001 cctgctgcct ggtaaataac atcagcacct gctgcatact gagctgctgc gattgttttt
     6061 ccttttgcag cgtcaccaaa tgatccagca taatcaactt taacttggat tgtatcgtca
     6121 acagacttaa ctcctgcttc aaaacctttt tcaaatcgag ttatgacagt tccttccata
     6181 ccgcccacga aaccaactgt ttttgttttt gttgtttttg cagctgcaat tcctgcaaga
     6241 taagcagctt catggtcggc aaaggtaaca ctggctacat tatcttttcc ttcgataata
     6301 tcatcgataa taacaaactt aactccttca ttatctccag ctgctttagc aatagcatct
     6361 ttcaatgcaa agccgatacc ataaatcagt tgataccctc ctgaaactgc tgtatcgaga
     6421 ttagttgcat actcagattc acttgtagat tgaaaataat cgaaacctgt tcctttttga
     6481 aggcccattt ctttacccca agattgcagg ccttcccatg ctgattggtt gaatgatttg
     6541 tcatctacac caccagtatc ggtaaccatt gcaactttta aatcagtttt tcctgatgcc
     6601 ccacctttag aagcaccacg attaccacaa gcagctaaac tcagcacagc cactgacgct
     6661 aaaccaagac caataaattt cttgttcatg aatatgaacc ctcctaaaaa agatttctag
     6721 caaataagtt gctgaaagta aatataaagc caagacggct agataacaga aattaaagca
     6781 gattatatta aatctgtaaa agaatagggg agtaaatctc caacagtcat ttcaacagtt
     6841 tgaccatttt tagcaattaa agtgactaaa gaagaaggag caaaaaattc tgccattacc
     6901 tgacgacaag ccccacaagg agatacaggt tgcatagttt ctccataaat agcaatttca
     6961 accaattctt tatgaccatc agatatggct ttaaatatag ctgttcgctc tccacaattt
     7021 gtcagaccaa aactaacatt ttcaatatta cagccagtat agatcgttcc atcttttgtt
     7081 tttaaagctg ctcctactgg aaaatgagag taagggacat aagcatactc actagcttgt
     7141 acagcacaac taactaaatc agtagtcccc atttaacatc acctttcata atagctacat
     7201 ctattgaaac ccctgtagat actttaacga aatatgcacc cgacttgata gataattaac
     7261 gtgatctaac tttagcactt atatcatctt cgatagactg gtaatttctt tgctttgaag
     7321 taaacgaccg ttcaaaacga tatcaatctc ttcagcactg tttgaattaa catctttaac
     7381 ttcaactagt tcactggtat ccgacgtttc tccaaaaaga aagactataa cggtcccgtc
     7441 agtacattaa cgtcgacacc ttagaagcat agactattta acatggattg atacagatat
     7501 ttgtaaaatt ataaatatct gtatcaatca cttgatcaat ctgtgatcga acgctaccta
     7561 tctttagtag cgtatggttg attgtcacca taacaatgac accttagaag ataatgat
//
LOCUS       DATURV010000063         7538 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DATURV010000063 DATURV010000000
VERSION     DATURV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.063"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..819
                     /locus_tag="VCF75_001395"
     CDS             <1..819
                     /locus_tag="VCF75_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEP2546948.1"
                     /translation="LLSLFADPWLLAVHLLDILIVAYLIYRFIKALTGTKIMSLVQGV
                     IFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEKFGRSTQVFLQK
                     QYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPLNADISSQLLIN
                     IFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAIGLSENSDALTI
                     IVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKKILGGKSK"
     gene            816..1772
                     /locus_tag="VCF75_001396"
     CDS             816..1772
                     /locus_tag="VCF75_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEP2546949.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVSVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            1826..3181
                     /locus_tag="VCF75_001397"
     CDS             1826..3181
                     /locus_tag="VCF75_001397"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEP2546950.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     AKIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            3305..3946
                     /locus_tag="VCF75_001398"
     CDS             3305..3946
                     /locus_tag="VCF75_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2546951.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTQKQDT
                     QNRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQEKLPSLSIMVSQESSKLFYQEQGFVLHAKGCKPGNENDSDVCQLLKYFSKP"
     gene            4009..5139
                     /locus_tag="VCF75_001399"
     CDS             4009..5139
                     /locus_tag="VCF75_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEP2546952.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTITNLKAAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGMSFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            5149..5901
                     /locus_tag="VCF75_001400"
     CDS             5149..5901
                     /locus_tag="VCF75_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEP2546953.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGD
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKLHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESETD"
     gene            5901..6665
                     /locus_tag="VCF75_001401"
     CDS             5901..6665
                     /locus_tag="VCF75_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEP2546954.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQERGIPYLLVTNN
                     TTRTPEMVQSMLANQFHVETSIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            6665..7297
                     /locus_tag="VCF75_001402"
     CDS             6665..7297
                     /locus_tag="VCF75_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEP2546955.1"
                     /translation="MVENTKLLCSWGWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT         2316 a         1327 c         1601 g         2294 t
ORIGIN      
        1 ttattaagct tatttgcgga tccttggctg ctagcagtgc atcttttaga tattctaatt
       61 gttgcttatt tgatttatcg ttttattaaa gcattgactg gaactaaaat catgtcttta
      121 gttcaagggg tcattttctt tttggttctt cgttttattg cagagtggat tggctttacg
      181 acaatcacat acttaatgaa ccaagtcatt acttatggtg taattgcagg agtagtcatt
      241 tttacccctg aaattcgggc aggactggag aaatttggtc gttctacgca agttttttta
      301 caaaaacagt acgtcagcag tgaatctgct ttggtcgatg ctttgattaa atctgtggcc
      361 tatatgggtc cacgcaagat aggtgcactg attgctattg aacagactca aaccttacaa
      421 gaatatattg cgacaggaat tccgctgaat gccgatattt caagtcaact actcatcaat
      481 atttttattc caaatacgcc gcttcatgat ggagctgtta tcgttggtca gaataagatt
      541 gtggcagctt gtgcctactt gcccttgtca gaatcaaagg caatttccaa agaatttggg
      601 acccgtcata gagcggctat tggattatct gaaaactctg atgcactaac tatcattgtt
      661 tcagaagaaa caggagctat ttcagtaact cgcaaaggcc aatttttaca cgatttaagc
      721 acagatgaat ttgaaacggt acttaggacc tacctgatga gtgattcaaa tgttagttta
      781 ccatggtata agaaaattct aggagggaaa tctaaatgaa acgattttta aatagtcgtc
      841 cttggttagg aatggtatca gtcttttttg cgattctcct ttttttaact gcagcatcga
      901 gcaatcataa taactctagc tcgcaaattt atagtccgat tgaaacctat acgcatagcc
      961 ttaaagatgt accaattgat atgaagtatg atagtgacaa atattttatc agtgggtatt
     1021 cttatggtgc ggaagtttat ctaacctcca ctaatcgtat caaattagat tctgaagtga
     1081 acaatgatac acgtaatttt aagattgtgg cggatttaac acatagtcat ccaggtacag
     1141 taagtgtcaa cctaagagta gaaaacctcc cttctggggt gacagctacc gtatctccag
     1201 ataaaatatc agtcacaatt ggcaaaaaag aatctaaggt atttcctgtt agaggaagcg
     1261 ttgatgctaa acaaattgca aatggttatg aaattagcaa aattgaaacg ggggttaata
     1321 aggttgaagt gaccagtgat gaatctacca ttgctttaat tgatcacgtt gtggcaaagt
     1381 tacctgatga tcaagtgtta gataggaatt atagtagtcg agtgacctta caagctgtct
     1441 cagctgatgg aactatttta gcaagtgcaa ttgatcctgc aaaaacaaat ttatcagttg
     1501 ctgtaaaaaa aataacgaaa tcagtcccga ttagagttga ggcagtgggg atgatggatg
     1561 atagcttatc agatattcaa tacaaattgt caaaacaaac agctgttatc tcaggtagtc
     1621 gagaggtctt agaagatatt gatgaaatta ttgcagaagt taacatttct gatgtcacta
     1681 aaaatactag taagaccgtg agtttgtctt ctagtcaggt gtcaattgag ccgtcagtag
     1741 tgaccgttca gttgacaacc accaaaaaat aatggataat aactaggaaa attgaatgga
     1801 ggaagccatt aggcttatat aaattatggg aaaatatttt ggaacagatg gtgttcgtgg
     1861 cgaagcaaat gttgagctga cacctgagtt agcttttaaa ctgggccgat tcggtggtta
     1921 tgttttaagt caacatgaga ccgaaagacc taaagttttt gtggcgcgtg acacccgtat
     1981 ttcaggagaa atgttggagt cagccttgat tgctggtcta ctatctgttg gtattgaagt
     2041 ttataaactt ggcgttttag cgacacctgg cgtctcttat ttagttagaa ctgaaaaagc
     2101 aagtgcaggt gtgatgattt ctgcaagtca taaccctgcg cttgacaatg gcatcaaatt
     2161 ctttgggaat gatgggttta agttagctga tgaccaagaa ttggaaattg aagctctctt
     2221 agatgctccg gaggacacgc ttcctcgccc gagtgcagaa ggacttggaa ccttggtaga
     2281 ttatccagaa ggacttcgta aatatgaaaa gttcttggta acaacaggta ctgacttaag
     2341 cggtatgaca gttgccttag acactgctaa tggagctgct tcagtgtcag cacgtgatgt
     2401 ctttttagat ttaaatgcag aaattgctgt cattggtgaa aaaccaaatg gtcttaatat
     2461 taatgacggt gttggttcaa cgcatccaga acaacttcaa gagttagtta aggaaacagg
     2521 agcagatctt ggtttagcct ttgatggcga tagcgaccgt ctgattgctg tggacgaaac
     2581 tggcgagatt gttgacggtg atcgtattat gtttatcatt ggtaaatatt tgtcagaaaa
     2641 agggcttctt gctcacaata ccattgtcac aacggtgatg tctaacctcg gttttcataa
     2701 agctttagat aaacaaggta ttaataaagc aattacagca gttggagacc gctatgtggt
     2761 tgaagaaatg cgtagctcag gatacaatct cggtggtgaa caatcaggcc atgtcattat
     2821 catggactac aataccactg gagatggtca gttgacagct atccaattag ccaaggtgat
     2881 gaaggaaact gggaaatcac tttcggaatt agctgctgaa gtaaccattt accctcaaaa
     2941 actggtgaat attcgtgtgg aaaacagcat gaaagaccgt gctatggaag ttccagctat
     3001 tgcgaagatc attgcaaaaa tggaggatga aatggctggt aatggtcgta ttctcgtgag
     3061 accaagtggt acagaacctc ttcttcgtgt catggcagag gcgccaacag atgccgaagt
     3121 tgattattat gtcgatacta ttgctgatgt cgtccgtaca gaaatcggat gcgataacta
     3181 acgagttgag tacaaacaag aaaccttatt tctaaggctt ctgtcaacaa ttggaaagag
     3241 gcttggggcg tttagcccaa gtctcttttt ataaattact aaagtagaag agaaaggata
     3301 tcggatgaca aaacagcaaa tcgaaaaact agcggactta ctgcctcctt ttgcagccac
     3361 actttactta gatatgattc ctgaatttca agaaggttat gcttcttatg tttatcaaac
     3421 acaaaaacaa gatactcaaa accgtcgcat caagcagtta actaaaaatt ttgaacgatt
     3481 actagatacc ccatcttttt atgtgatgcc tgtgacaaga aaaacagcag agcaattgct
     3541 agcagataaa ggcctagctt atcatttgtt atcgacagtt gcagctaatc aagtgtttgc
     3601 ggctgctgtt aacgaggatc agacgctttt tcaagtgctt cgaaatggag caagagtagc
     3661 ctttgtgatt cttcaagaaa agggggaatt tctagaaatg actttaggga ttcttgaaac
     3721 agtgacagaa aacgaggttc cagcagctat tgtggaggct gtccagacat atcttctggc
     3781 agatatgcaa gagaaactcc cttctttaag cattatggtc agtcaagaat caagcaagct
     3841 tttttaccaa gaacaagggt ttgttttgca cgctaagggt tgcaagccag gaaacgaaaa
     3901 tgattctgac gtttgtcagc tcttaaaata cttttccaag ccatgaggaa tcaagggaaa
     3961 tggtttattc atagaaacat tttatcatca aagtggtata ataagaccat gtcaaaaaaa
     4021 cctacttcag cctatgtgca cattcctttt tgcacacaaa tttgttatta ttgcgatttt
     4081 tcaaaagtct ttatccaaaa tcaacctgtt gatgcttatt tgaaagcctt gattcaagaa
     4141 tttgactctt atggcattcg agacctaaaa accctttata ttggaggagg tacgcctaca
     4201 gctatcactg ctaaacagtt ggagtatctc ctaaatcacc ttgaacgaaa cctcaattta
     4261 gatgatcttg aagaattcac aattgaggca aatcctggtg atttgacgcc agaaaaaatt
     4321 gcggtgcttc agaggtcagc tgtcaatcgg atttccttgg gggtgcagac ttttaataac
     4381 aagcaattaa agcagattgg ccgtagccat aatgaagaac aaatttatag caccatcact
     4441 aatttaaaag ctgcaggctt tcacaatatt tccattgatt taatttatgc cttgccaggt
     4501 caaaccttag atcaagtcaa ggaaaatgtt gctaaagctt tggcacttga tattccccat
     4561 cttagtcttt atagccttat tttggaacat cacaccgttt ttatgaacaa aatgcggcgt
     4621 ggtaaactta acttaccaac ggaagacttg gaagcggaaa tgtttgagta tattatttca
     4681 gaaatggaag ctaatggttt tgagcattat gaaatttcca attttaccaa accaggtttt
     4741 gaaagtcgcc ataacttgat gtattgggat aatgtggagt attttggatg tggtgcaggg
     4801 gcttcaggtt accttaatgg cattcgttat caaaatcggg tgcccatcca gcattatctt
     4861 aaagcggttg aagcaggaaa cgctagacta aatgaagaag tgctgcgcaa agaggaaatg
     4921 atggaagagg aactcttttt gggcttacgt aaaaagacag gagtctccat ccagcggttc
     4981 caagaaaaat ttggtatgtc ttttgaggag cgttatggaa atattgttag ggagcttcaa
     5041 aaccaaggac tgcttgtcaa agatgacgct tttgtcagaa tgactaaaaa gggtcttttc
     5101 ttaggtgatt ctgttgctga gcggtttata ttagattagg agagtattat gggattaagt
     5161 tatcaggagg agttgacact tccttttgaa ttatgtgatg tcaaatcaga tataaaattg
     5221 ccccttttat tagactattg tttgatggtt tctggtagac agtctgcgca attaggacga
     5281 agtaacaaca atcttttagt cgattacaag cttgtttgga ttgtaacgga ttatgagatc
     5341 actattcatc gcttgccaca ttttcaagaa accatcacca ttgaaacaaa agccctctcc
     5401 tacaataaat ttttttgtta tcgccaattt tatatttatg atcaagaggg ggatctttta
     5461 gtggatatct tagcctattt tgctttgtta aacccagata cgcgaaaagt ggcaactatt
     5521 ccagaagatt tagtagcgcc ttttaagact gattttgtta aaaagctaca ccgtgttcct
     5581 aaaatgcctc ttttagaaca atcaattgat cgtgattatt atgtgcgtta ttttgatatt
     5641 gatatgaatg gtcatgtcaa caacagtaaa tatttagatt ggatgtatga tgtgttgggg
     5701 tgtgagtttt taaaaacgca tcagcctctt aagatgactt tgaaatatgt taaagaagtc
     5761 tcaccaggcg gtcaaattac ttccagttac catttggacc aattaacttc ttaccatcaa
     5821 atcacctcag atgggcagct gaatgcccaa gccatgattg aatggcgagc gattaaacaa
     5881 acagaaagcg agacagacta gtgccttata agggttattt aattgattta gacggaacga
     5941 tttaccaagg gaaaaaccgt attccagcag gggagcggtt tatcaaacgc ctacaagaaa
     6001 gaggcattcc ctatttatta gtgactaata ataccacaag aacacccgaa atggtgcaaa
     6061 gcatgctggc taatcagttt catgttgaga caagcattga gaccatttat acggctacca
     6121 tggcaaccgt cgattacatg aatgacatga accgtggcaa gacagcttac gttattggag
     6181 aaacagggct caaatcagcg attgcagcag caggttacgt agaagaactt gagaatcccg
     6241 cttacgtggt ggttggttta gacagtcagg tgacctatga gatgttggct atagccacct
     6301 tggctattca aaaaggtgcc ctctttattg ggacaaatcc tgatttaaac attccaaccg
     6361 aacgtggttt gatgccagga gcaggtgcat tgaatgcctt actagaagct gccacacgtg
     6421 taaaacctgt ttttattggc aagcccaacg ctatcattat gaataaatca ctagaggtat
     6481 tgggcattca gcgctctgaa gccgttatgg ttggcgataa ttacttgact gatatcatgg
     6541 caggtattca aaatgatatt gcaacgatcc tcgtgacaac aggctttacc agacctgaag
     6601 aggtaccaac cttaccaatt cagccagatc atgtcctatc tagtcttgat gagtggaggt
     6661 tgtgatggta gaaaacacta aattactatg tagctgggga tggttattag cattagctat
     6721 tctcataacg atctattcaa cgtggctgtg gtatcctctt gaggtggacc atctaaagtt
     6781 agaacaagtt gtttttatga gcaaggatgc tatcttgcat aactataacg gactactaaa
     6841 ttaccttacc aatccttttg taacaaggtt ggaatttgct aattttcact cgtcagcaga
     6901 cggactcaag cattttgcag atgtgaagtg gctttttcac ctaactcaag tcgtttttct
     6961 tggcctcctt tacccaaccc ttaagacttt cacacaaagg ttaaaaacca aacgtttctg
     7021 gctccttcaa aaacctttaa tactagcagc tttgtttcct cttatgattg gtctaatggc
     7081 tagtttcatt gggtttgaac atttttttac tctttttcat caggtgcttt ttgtaggaga
     7141 tagcagctgg ctctttgatc ccttgaaaga tcctgtgatt tggatccttc cagaagtctt
     7201 tttcttgcac tgtttccttt tctttatgat agtttacgaa atcatcctgt ggagcttagt
     7261 aggtttagca agatggcagc gcctaaatgt agaatgataa aaggattaag agattaattt
     7321 ccctaaaaat gataaaacaa gcgttttgaa agcgcttgtt ttttagtttg cagtcagagt
     7381 agaatagaag tatcaaaaaa acaccgactc ggtgccactt tttcaagttg ataacggact
     7441 agccttattt taacttgcta tgctgttttg aatggttcca acaagattat tttataactt
     7501 ttataacaaa taatcaagga gaaattcaaa gaaattta
//
LOCUS       DATURV010000064         7520 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DATURV010000064 DATURV010000000
VERSION     DATURV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.064"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            87..1505
                     /locus_tag="VCF75_001403"
     CDS             87..1505
                     /locus_tag="VCF75_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP2546956.1"
                     /translation="MDKKIQRFSCTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMI
                     VVKPEDQPRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATE
                     TITTNSRVLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVA
                     FSDEHDIWWLETIGGHHWIARRVPDDAYVTNPNQFGIDHFEFNNPEDYLCSADLKDFI
                     DTYHLDLTYSHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWC
                     QKPYRKITVEDVKYVLSSHYQDTGYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRP
                     NKPQEIAAIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAAL
                     ADPHYNHHETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQ
                     TLLNKILFDASNLMTNRFSLSD"
     gene            1657..3204
                     /locus_tag="VCF75_001404"
     CDS             1657..3204
                     /locus_tag="VCF75_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEP2546957.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPIYEF
                     TKGIVGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDTDAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGAGHDHHHEDADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTKKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGERKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            3355..4077
                     /locus_tag="VCF75_001405"
     CDS             3355..4077
                     /locus_tag="VCF75_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2546958.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMIENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKVGKTPKTEILS
                     FEQYQVTPYLSGLLEFDPDTEVFELERLRIADDMPLMFERSYIPAQLFQGLSIEHLKR
                     KPMYDLFAEDYQETIRLAEEEFYASIALDYEAGLLGIKKGDPVLHIIRKTYNDNNILI
                     ELTFSIARADQFRYRVQHHPNG"
     gene            4096..5295
                     /locus_tag="VCF75_001406"
     CDS             4096..5295
                     /locus_tag="VCF75_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEP2546959.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFDETRDSLDS
                     FLKRVCNSANRNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQRYLFESVASTDLVAAP
                     DYYLVEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQYNKA"
     gene            complement(5363..5623)
                     /locus_tag="VCF75_001407"
     CDS             complement(5363..5623)
                     /locus_tag="VCF75_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEP2546960.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(5738..6679)
                     /locus_tag="VCF75_001408"
     CDS             complement(5738..6679)
                     /locus_tag="VCF75_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEP2546961.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLAWMSQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            7073..>7520
                     /locus_tag="VCF75_001409"
     CDS             7073..>7520
                     /locus_tag="VCF75_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEP2546962.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSEQFALTGAIKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
BASE COUNT         2359 a         1392 c         1572 g         2197 t
ORIGIN      
        1 taaggaggtg ctgctggaat tatgaaaatt tttgtatcca gttcaatgga tatttgatat
       61 aatcgtctta aaaaggaggt cgtgaaatgg ataaaaaaat acagcgtttt tcatgtacaa
      121 ctatactcgt cggtaaaaag gcatcttatg atggctcaac catggttgct cgaacagaag
      181 attctcaaaa tggtgatttc acgcctaaaa aaatgattgt ggtgaaacca gaagatcaac
      241 cgagacatta ccgttcggtg caatcttcct ttgaaatgga cttaccggac aatcccatga
      301 cttacacgtc tgttcccgat gcgcttggta aagatggtat ctgggcagaa gcaggcgtga
      361 acgaggctaa tgtggccatg tcagcaactg aaaccatcac taccaattct cgtgttttag
      421 gggcagatcc tctagtagct tctggtattg gtgaggagga catggtgacc ttagtgctcc
      481 cttatatccg ttctgcgcgt gaaggggttt tgcgccttgg tgccatttta gaagactatg
      541 gcacttacga gtcaaatggt gtggcttttt cagatgaaca tgacatttgg tggctggaaa
      601 cgattggtgg tcatcactgg attgcaagac gggtgccaga tgatgcttat gtcaccaatc
      661 ccaatcaatt tggcattgat cattttgaat ttaacaatcc tgaagattac ctttgctcag
      721 ctgatttaaa ggactttatt gacacgtatc acttagattt aacctatagc catgagcatt
      781 ttaatccgcg ctatgctttt ggtagtcaac gtgacaaaga ccgtcactat aatacgccac
      841 gtgcttggat catgcaaaaa ttcttaaacc cagaaattgt gcaagacccg cgcagctttg
      901 cccttgcttg gtgtcaaaaa ccttaccgta aaataacagt agaagatgtc aaatatgtat
      961 tgagtagtca ctatcaggac acgggttatg acccttacgg ttcagaagga actcctgtta
     1021 gtaagaaagt ctttcgccct attggcatta accgtaccag ccaaaccgct atcttacaca
     1081 ttcgtcctaa taaacctcaa gaaattgccg ctattcaatg gatggcttat gggtcaatgc
     1141 catttaatac catggtgcct tttttcacac aggtgaagac tattccagat tattttgcca
     1201 acacttacga aaatgtgtct accgacaatt tctattggac caaccgtctg attgcggctt
     1261 tggcagatcc tcactacaat catcatgaaa ctgatttgga caattacttg gaagaaacca
     1321 tggcaaaagg tcatgccatg ttacatgctg tggaagcaca gttattggct ggtgaaacag
     1381 tcgatttgga agaggaaaat caaaaaatga gtgactatgt gcaaggagaa acgcagacgc
     1441 tgttgaacaa gattctcttt gacgctagca atctaatgac caatcgtttc agtttgagcg
     1501 attaatagtg aagctaaggc tgagcaactg cttagccttt tttacgataa ctttttagga
     1561 aaagtaatcg caaaaagaca aaaacttgga aaaaaaatca aagtatgcta tacttaactg
     1621 gtcaattttt aaccagttaa taacaaagga ggaatcatga aaaagaaaat tcttttaatg
     1681 atgagtttaa tcagtgtctt ttttgcttgg caacttactc aggcaaaaca agtcttagca
     1741 gagggtaaag tgaaggtggt gacaactttc tatcctattt atgaatttac aaaagggatt
     1801 gttggtaatg atggcgatgt ttccatgctt atgaaagcag gaacggaacc tcatgatttt
     1861 gagccttcta caaaagacat taaaaaaatc caagatacag atgcatttgt ttatatggat
     1921 gacaatatgg aaacttgggt ttctgatgtg aaaaaatcat tgacatctaa aaaagtgacc
     1981 atcgtcaagg gaactggtaa catgctcttg gtagcaggag ctggacatga ccatcaccat
     2041 gaggatgctg acaaaaagca tgagcataat aaacatagtg aagaaggaca caaccatgct
     2101 tttgacccac acgtgtggtt gtcaccatac cgtagcatta cggtcgttga aaatattcgc
     2161 gacagtcttt caaaagctta cccagaaaaa gcagagaact tcaaagccaa tgccgctact
     2221 tatattgaaa aattaaaaga gcttgacaaa gactatacgg cagcactttc agatgctaag
     2281 caaaagagct ttgtgacaca acacgcagct tttggttata tggcacttga ctatggcttg
     2341 aaccaaattt ctattaatgg tgtcacacca gatgcagaac catcagcaaa acgtattgct
     2401 actttgtcaa aatacgttaa aaaatatggc atcaaataca tttattttga ggaaaatgcg
     2461 tcaagtaaag tcgcaaaaac cctagctaaa gaagcaggag ttaaagcggc tgtgcttagt
     2521 ccgcttgaag gtttgactaa aaaagagatg aaagctggcc aagattactt tacggtcatg
     2581 cgtaaaaacc ttgaaacctt acgcttaacc actgatgtgg ctggtaaaga aattcttcca
     2641 gaaaaagata cgacaaagac agtttacaat ggttatttca aagacaaaga agtcaaagat
     2701 cgtcaattat ctgactggtc aggtagctgg caatctgttt atccctatct acaagatggt
     2761 actttagacc aagtttggga ctacaaggct aaaaaatcca aaggtaaaat gacagcagcc
     2821 gagtacaaag attactacac tactggttat aaaactgacg tggaacaaat caaaatcaat
     2881 ggtaagaaaa agaccatgac ctttgttcgt aatggtgaaa ggaaaacctt cacttacaca
     2941 tacgccggca aagaaatctt gacctatcca aaaggaaatc gcggagttcg tttcatgttt
     3001 gaagctaaag aaccaaatgc tggcgaattc aaatacgttc aattcagtga ccatgccatt
     3061 gctcctgaaa aagcagagca tttccacctg tactggggtg gtgacagcca agaaaaatta
     3121 cataaagagt tagaacattg gccaacttac tacggttcag acttatctgg tcgtgaaatt
     3181 gcccaagaaa tcaatgctca ttaatcttaa ctcactaagc aaatcttctt ttaagtaagg
     3241 aggtttgctt tttctttgtg gagtaaaaaa attaataatt ctcaacatta atttgacgga
     3301 cacttatgat tataatatac tttaagtggt tataacaagt tagaggtgaa taagatgcca
     3361 aaagaacaac cactctatct acagattgtt gatgacttgg aagtgaaaat caaaaaaagc
     3421 atgatagaaa acgataagtt gttgtcagaa cgagaattaa gtgaccttta cggtgttagt
     3481 cgtatcacga ttagactggc tcttaaggaa ttggaattac gagacttaat ttataaaaag
     3541 cagggcaagg ggacttatgt ttcaggaatc aaagaacctg tcactgattt atcaagtact
     3601 tacagcttta cagagcaaat gaaaaaagtg gggaaaactc ctaaaacaga gattctttct
     3661 tttgaacagt atcaggtaac accttacttg tctggtctct tagagtttga tcccgataca
     3721 gaagtttttg aattagaacg cctaaggatt gcagatgata tgcccttgat gtttgaacga
     3781 tcctatattc ctgctcagct ttttcaaggg ttaagcattg agcacttgaa gcgtaagcca
     3841 atgtacgatt tatttgcaga ggattaccaa gaaactattc gcctagcaga agaagagttt
     3901 tatgctagca ttgccttgga ctatgaagca ggtctcttag gcattaaaaa gggagatcct
     3961 gtcttacata ttattcgtaa aacttataat gataacaata tcttaattga attgactttc
     4021 agcattgcaa gggcggatca gtttaggtac cgagtacaac atcaccctaa tggttaagga
     4081 attggaggtt aggacatgtt tacaaaaacg caggaagctt tggaggctct tggtgcagct
     4141 atcactacca aggaaattaa acaagaaccc cgtttatggc aagaaaccat gacgtttttt
     4201 gacgaaacaa gagattctct ggattctttt ttaaagagag tttgtaacag cgctaacaga
     4261 aataacgttc atgtcatctt tacaggtgcg ggtacatctg agtacatcgg taataccatt
     4321 tgcccttact tgaagaaggt ggggaatcgg cagcgctatc tctttgagag cgttgcttca
     4381 accgatttgg ttgcggcgcc agattattat cttgttgagg aagagacggt actattagtc
     4441 tcctttgcta ggagtggtaa tagtccagaa agtgtagcgg ctgttaacct ggtcaatcaa
     4501 ctagtgccaa atagttatca tttaaccatt acctgcgcca aagatggaga actggcgaaa
     4561 aaagcacagc aagatgagcg ttcttacctt tatttgatgc ctgaagacgc taatgatgct
     4621 ggttttgcca tgactggtag ttttacctgt atgatgctag cggctctttt gatttttgat
     4681 gatgctaaca gtatagctca gaaagaagct tatgtgactg atatgattct tgctgggcaa
     4741 gctgttattg accaagaaag acggctacaa gagctagctg atttaggatt tgaacgttta
     4801 gtttatctag gttctgctgg tttagccaag ttaacccaag aagctcaatt aaaaatgtta
     4861 gaattgacag ctggacaagt tgcgaccctc tacgagtcct ctatgggctt taggcatggt
     4921 ccgaaatctt ttattaatga ccgtacctta gtcattggat ttgtcaataa tgatgcttac
     4981 gtcagacagt acgatttgga catgttagag gaaatccaag cagatggcat tgcccttaaa
     5041 atcttggctc tattgcaagg aggggatatc aacttttcag aggaccaatt tcgactagac
     5101 acccgtcact tattgccaga agcttactta gcttttccca tgattttagt cgcgcagacc
     5161 ttggccctct taacggccgt tacattagga aattcccctg acaccccatc tgcgacagga
     5221 acggttaatc gtgtggtcaa aggagtaacg attcaccctt acccgactag tagtcgtcaa
     5281 tacaacaaag cttaaagctt ttgtgctagt aggccaaaag aaaaaccctc ctgatacagg
     5341 aaggtttttt gtattgtctt gcttattttg ctgcgtttgc gtctttgaga ccgtattttt
     5401 tgttgaagcg gtccacacgt ccatctgctt gagtgaactt ttgacgtcct gtgtagaatg
     5461 ggtgtgaatc tgatgaaatt tccacacgga taagtgggta agtttcacct tcaaactcaa
     5521 cagtttcttt agaagctttt gttgagccgc taaggaactg gtaacctgta gttgtatcaa
     5581 ggaaaacaac tggacgataa tctggatgaa tgtcttttct catcttaaaa aattcctttc
     5641 tgccatgaca cttgatgcat catagttatt aactattcta gtgtaacaaa acttgaatga
     5701 cttgacaagg cttttctttc cttatttttt aaaaatccta aatctcttgg caaacagcga
     5761 tgagttctcg gaaaatagct tgattttctt ctaaattagc tgagttggct ccgctagcaa
     5821 ggggatgccc ccctccaccg tgacgcttag caatgccatt aataatcttt tctttactgc
     5881 gcatacgcac acggtagttg ccgtcagtta actcaacaaa aatagcccaa gcttgaacgt
     5941 tatcaatttt accaggagca cagacaatag cagagctttc tgctagggtc acgtcaaaat
     6001 gttttaaggt ttcttggctg acaaggacat aagcagcccc actctcatca attgttaaat
     6061 gctcaaagac gtagctttgc agctttgcta ttttcaaagg aaacgaatcc atttgccttg
     6121 aaatcgcagc aaagtcgaac tcgaaatgtc tgagttggct agcaatggaa agggttttac
     6181 tagtggttga ggcataaaga aatcgccctg tatcaccaac gataccagtg tataagagct
     6241 tagcagcttt gtcagagaga gtaagattct gactaaaggc aaagtctgca atgatttcac
     6301 ttgcgctaga agcgcttgtg tccacataat agaagtcacc atacacatca tcgttgggat
     6361 ggtgatcaat cttaattaag cactgcccca gtgtgtagcg ctcatcatca atccttggtc
     6421 gattcgctgt atctgtaatg atgaccaaag cctctttgta gtctttgtca gtcacctgat
     6481 ccatttggct catccaagct aaactaggct catcaaaacc agtcattaaa acctttttgt
     6541 ctgggaaatt ttgtgcaata atttctttca agccggcctg actaccaaga gcatcagggt
     6601 caggattttg atggcgatgg ataataatag tttggtgagc ttttatttta tctaaaattg
     6661 tttcaaaagt tgttatcatg ctttaatctt tctttttcaa aaagaggaaa ataaccgttt
     6721 aacatagtct tcaaaaaacg gtttgtgtca taaggaggtg tgcgttatgt tcagtggcta
     6781 atgctagaat tttttaagat tgtcaaagac cataaattga cattttatct gaaaatcata
     6841 tcctatttta ttatagcata aaagtaagct ctagataatg gtaaattaat ctaggaagaa
     6901 aagaggttag ttatcagcta tgatactatc aaagctagtc ttgcaaatgc aagtgaaaaa
     6961 atagttttta agtgttttaa gtaatcatat caaccttctc gttaaagaat tagtgctttt
     7021 cctaaaaaag tgtgctaaaa taaacatagt ttagtaattt tgggaggaat ttatggcatt
     7081 agcaaaaatc gtttatgcca gcatgacggg aaatactgaa gaaatcgctg atatcgttgc
     7141 aaataaattg caagaattgg gccatgatgt tgacattgat gagtgcacaa cagttgacgc
     7201 ttcagagttt gagaatgctg atatcgctgt cgttgcgact tatacttatg gtgatggtga
     7261 cttaccagat gaaatcgttg atttttacga agatttacaa gatttagatt tagaaggtaa
     7321 aatctatggc gttgtcggct ctggtgacac tttttatgac tatttttgca aatctgttga
     7381 tgacttttca gagcaatttg cgcttactgg tgccatcaaa ggtgcagagc cagtcaaagt
     7441 agatttggca gcagaagatg aagacatcga tcgtttagag gcctttgcag agcaactctc
     7501 acaggctgtt aataatctat
//
LOCUS       DATURV010000065         7307 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DATURV010000065 DATURV010000000
VERSION     DATURV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.065"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(705..1628)
                     /locus_tag="VCF75_001410"
     CDS             complement(705..1628)
                     /locus_tag="VCF75_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546963.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1621..2691)
                     /locus_tag="VCF75_001411"
     CDS             complement(1621..2691)
                     /locus_tag="VCF75_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546964.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2700..3626)
                     /locus_tag="VCF75_001412"
     CDS             complement(2700..3626)
                     /locus_tag="VCF75_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546965.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3626..5128)
                     /locus_tag="VCF75_001413"
     CDS             complement(3626..5128)
                     /locus_tag="VCF75_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546966.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYKKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMVGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5193..7163)
                     /locus_tag="VCF75_001414"
     CDS             complement(5193..7163)
                     /locus_tag="VCF75_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP2546967.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2283 a         1412 c         1349 g         2263 t
ORIGIN      
        1 ctttttttgt acttttttat ttgcccgagt tgtaaaatga agtgcaacaa aaaagaggaa
       61 aagccttgaa ctacacccca aacgttagac aaaaaaatct aacgttcggg gtatttttta
      121 tgaaattaag tgatgaaggt acgaaataga catcgttaaa aaaaatacct actatccacc
      181 agagataaag caggaaataa tggataacgt tttgatagaa ggcccgccac aataccaagt
      241 atcacttgtt tatggcttgt tacatgcagg aactctccca agttgaatag cacaatataa
      301 gaaaaacagt tatactgttg agaaaacaag agcaagacca tcacaaacag gacgtaaacc
      361 aaaagaaacc tgaagaaatg actaaattag agcggcttca agaagaaaat gaatatctca
      421 gaactgaggt tgcttactta aaaaaaccga gggaactccg tttgaaagaa gcatctctgc
      481 aaaaaagaag acaaaaaacc attataaaga atggacaaag acgagctctg attagatttt
      541 cttttaagaa cggctaaatt gactcgttct acctactatt atcatcgatt attgcaacaa
      601 taaacgtatc aaaataaaac gaaaaggact tagtcctcta caatacaaaa ctaaatcctt
      661 tactaaaaat agagtgttca acttttgggg tcagtgcatc tcttttacaa ttctttttga
      721 tatttttcaa tttctgcttg gtttgcccaa acaaagtgat ttggtttgat ttcaaccatt
      781 gatggcttat ctaatgtata atcatgctgg tctggatgat agacaacaag ttctttttga
      841 cgctctaaaa ttggatctgg gataggcacg gctgataaca aagattgggt gtagggatga
      901 attgggttat taaacagttc ttctgtttct gcaacttcta caataacccc tttatggata
      961 accgcaatac gatctgaaat aaagcgaacg actgaaagat catgggcaat gaagagataa
     1021 gtcaaacctt tttcggcttg catccgtttg agaaggttta aaacctgtgc gcgaacggaa
     1081 acgtccaaag ctgaaatcgg ttcatcagca ataacgaatt cagggttcat tactagggct
     1141 ctagcgatac cgatccgctg acgttgacct cctgaaaatt catgagggta gcgcgtcaaa
     1201 tgctctgata gcaaaccaac ttcggccatc atgttcttaa ttttttcttt acgttcttcc
     1261 tctgttttaa acagattaaa gttataaaga ccttctgaaa tgatgtagtc aacggttgcc
     1321 cgttcattca aactagcagc gggatcttgg aaaatcattt gaattttacg aatgagctca
     1381 ttggcttctg attttgattt tctgccatta attactttcc catcgtataa aatttgacct
     1441 gaactagtat cgttcaaacc aataatagca cgaccaattg ttgttttacc actcccagat
     1501 tctccaacta aagaaaaggt ttctcctttt ttaataaaga aattagcatt tttaactgca
     1561 acaaattttt tctttccttc tccgaaggaa atttctaggt cttttacttc gactaatttc
     1621 tcagacattt ccttcctcct gttgtgacat tttccttaag attttttgat gcaaatcttg
     1681 aatgacttct ggtttttgaa cttttggagc ctctgggtgt aaaagccatg ttttggccca
     1741 atgagtctca gatacgttaa tcgccggagg tgctttttca aagtctaaaa ccatagcata
     1801 ttctgagcga agtgcaaagg catctccgat aattggtgaa taaagtgatg gaggcgttcc
     1861 tggaatagcg taaagttcac cagattcatc tgccaactgc ggtaagctag acagcaaact
     1921 ccatgtatag gggtgtcttg gatcatagaa aatctcttcg actgttccaa attcaacaat
     1981 ttctcctgca tacatgacag ccactttatc tgcaatgctt gccacaacac ctaaatcgtg
     2041 cgtaataaag ataatggtga aatgatattc tcgttgaagc gacttcaata actcaacgat
     2101 ttgagcttga atagtcacat caagggctgt tgttggctca tcacagataa gaatatctgg
     2161 acgacaagct aaagcaatag cgataacaat acgttgacgc attcctcctg aatactcaaa
     2221 tgggtaatct tcaaagcgtt ttttggcatt tgggataccc actttattca tgtaatcaag
     2281 ggccatttct ttagctttgg catgacttac tttttggtgc ttaataatca cttctgtgat
     2341 ttggctaccg atagttttaa tgggactaag actggtcatt gggtcttgga aaatcgttgc
     2401 gatttttgag ccgcgaatct ttgcccactc tttatttgtt tttaaatctg tcaattcttg
     2461 cccacgatag acaattgaac cattagcaat gcgtccatta gactccaaca tccctgtaaa
     2521 ggtctttgtt aaaacagatt tacctgagcc tgattcccct acaaaagcaa ggacttctcc
     2581 ttcaacaagt tccagtgaga cgttacggat agctgttaaa acacgatcac gcacatcaaa
     2641 ttctaccacc acatctttgg cagttaagat tacattattt tcttttgtca ttttaactcc
     2701 tatctatgtg aacgtgggtc actggcatca gccaagtttt gtccgacaat gtatagtggt
     2761 aacgatacta aaatcaatgt tactaaggga atccaaaaga ggtaggcatt tgttgttaag
     2821 ttgcttgaat aattagcaat caaacgtcct aaacttggag tggtggttgg taaaccaatc
     2881 ccaaagaagg ataagaaggc ctcagaagat acataaactg gtagcatttg tgacaacata
     2941 gtcatgataa ctgaaaccaa ttgaggcagg aggttcttaa cagcaatctt gtacattggt
     3001 gttcccaaag tttgactagc aaggttgtat tctaaatcac ggtaacgcaa gatttgaaca
     3061 cggatggagt aggcgacacc aatccatcca gtgatacaga aagctagaat caaattccaa
     3121 aatcctgcac ctaatgaata ggtcaaaaca atgataataa gcatagaagg gatatttgag
     3181 ataatgttat aaatttcaat cataacttta tcaaatgctt tagaaactcc ccatatggct
     3241 cctaacacta ccccaatggt gatattaatt agtgtcgcta taactgagat taaaatagaa
     3301 ttacgtgccc cataccaaac accatcaaac agagattgcc catttttgtc ggttccaaac
     3361 cagtactctg cattcggcca aatataacgc tttgaaaagt cattgatatt actaacgtca
     3421 ttaaagtcgt agttggcaaa cattggataa ataaagctca tcatcaagac tgtcactaaa
     3481 atcacgagca taaagactgt agatttttta gaaaaaaact gacgaaacac tgatttccag
     3541 taagaataag caggggtatc aatcacttcg gaggcttcac tatcgcgctc aacaaatcga
     3601 aatttagatt tatcaatcga ttccattact tacctccttt ttttgttgac aatttaatac
     3661 gtggatcgac aagggtcatg acaatatctc ctagtaaaag tgaaacaatt gacaagacag
     3721 tgaagataaa ggttaatcca acaatcatag aattgttagc agatttaata gaatcaatta
     3781 acattttacc catacccggg aaggcaaaga cagtctctgt taaggttgca ccaccaatag
     3841 caaggataat acttgcagga acccctgaca caattgggac catggcattt ttgaagagat
     3901 gccctctgta aatttcagat tctgataatc cttttgaacg cgcaaaacgt acccaatcgc
     3961 tggcctgaag gtcaacaaga tagcgacgga accagataac cgtagttgga atatttaaaa
     4021 ttcctaaaat aagggctggt aaaatataag atttaggatc agatgccccc agcattggga
     4081 aactatctgg taaaccaacc atgccaccta ggaagcgaac aacatagata acagcaatac
     4141 ttggtaaagc aagcatgaag gtcatggtcg ctgttgagaa acggtcaaaa taggtatttt
     4201 taaaccgagc cataaataaa ccaagaggca aaccaacgat ataggaaaac ataacaccaa
     4261 aaaaaccaat tacaaaagaa ttgtgaatca ttgatgggtc gacataatta ttgatggttt
     4321 tagtgtagga atctccctta ccatagttta ccttggtgat atcatccaat gattttggat
     4381 ttttgtagct acggctatag atatccactg acgatttctt agtgacacct gatgggaagg
     4441 tcacatcttg aagagctgtt cgtccttgcc cttgtgaaat aacctgaaga acaggaatat
     4501 tgctgtatgt tggataagat gttcctaagt ttagggtaac aaagttttgg tgaagataag
     4561 gaaattttcc gttagtatag aggagatatt tatgttttgt ccccgaacca acaagtgacc
     4621 agccaactga tttatctttt tctagacgaa tatacctagc taattttgga ttatctttat
     4681 cctgaatctt ccaagggtga tcaataacaa ctagatttga gaaaaagttc caaacacgtt
     4741 cgtaaatagg aatattgcga attgcataaa actgtttgct agtggggaag cgttttagct
     4801 gccagccatt gccaagagaa tcaatatact tcttatagat agctttgtta gcactagttg
     4861 cttctgtcgt tacactacta tctactttag aagcattatc ttttaattct ttgcttgaaa
     4921 aataattgac ataacccata cgttgaaaag ttagattttc atacgctact tttttatctg
     4981 gtgttgttgt cattttatta taattagggt cttgtttaaa gataaggctg gtaggtacta
     5041 gagtatatac gatggtgtat gttaaagccg ttaccaaaac taccgacact aacgatcgca
     5101 tgatacggtt taaaatgtat tttttcatag ttatcctcaa tagttcaaaa gaactccctc
     5161 acataaacga gaaagttctt ttgaacatcg ttttattttt caacgtgatc agctaacttc
     5221 tcagcatatt ttgcatttga cttagccttt gctttcatcc atttttcttt tgcattttcg
     5281 tattgttttg ctgtgacagg tttgtcttga agtttcattc ctttatatgc tagaggacct
     5341 ttagaccctg cccaagagaa gcccccgcta aatggaacgg ctttagtaac tcgtggagtg
     5401 ccacccaatg ccacaactgg aatatctacg gcattatctg taaggtaggc ttgtgctttt
     5461 gcgtaagctt tatagcgcgc atcgttgtcg tctgtaattg ctgctgcttc atcaagaaga
     5521 gtttggtagg tatcaagacc tgcagctgcc acaacatcct tattttgacc tgctttgatt
     5581 ccaagttttt ggataacaga tccaccacct actggactca tgatgtcaag gtaggtccgt
     5641 ggatcttgat agtctggtcc ccaccatgat gaaatgatat cgtagtcttg ttgttctggg
     5701 gtctcagcat agaagccttg ggcttcgtga gttgatgttt ctgtttcaag aacattgaca
     5761 atgacatttt ctttaccaag agatgcttca acagattgtt tgaaagactg ggcttcctga
     5821 acagttgctg cgtttgcttg gtcaacaggg taatctaatt gaactgggaa ggttacacct
     5881 tcagctgtca aagcttcttt ggcttttgca aactcagctt ttgctttttc aggattatag
     5941 aaaccatctt gagcatcagc taagttaacg tctttccatt catcaccaag ttttgccatt
     6001 tccttttcaa cttctgaacc aaaatcactt tctccaatgg tcacaaatgt tggtggcaca
     6061 agcatgttac gtaaggcttt tgttttggca tcttgacctg cagtttgggc ttggaatgac
     6121 gctcggtcaa aagcaaactg aatagcttga cgaaaatcct tgttgttaag agctttctta
     6181 ccggcatctt gttgtgcagg gtctttctta gtgtttttga aagaagtacg gttcaaattc
     6241 catgttaaat gacggatatc tccagtcaac attccgtaag taatgttatc agcatagttt
     6301 ttcttagctg atttgtaggt agggtcattt gggtaaagtc gtgcaacgct gaactcaccc
     6361 ttgtcaaagt tcttgtagaa cgaacctggg tctgaaccat ctgagtaagt caatttaaca
     6421 gattctatcc caacattctt agcatcccag tagttttcat ttttatggaa ttccatagat
     6481 gattttgagg tgaaggcgct caagaagtaa gcaccattaa caaggattga tgatggatcg
     6541 gttgtaccaa aatctttacc ttttgacttc aaaaatttcg cattaactgg gaaaagcaca
     6601 ctataagttg tttttgaatt ccagtagctt tcaggcttgt tcaaagtata ctgaacagtt
     6661 ttatcgtcaa gggctttgac accaacttct ttaaaatcta cttcaccatt ttggtaagcc
     6721 tttaagtttt ttattgaatc ttcaacaacg taaagagcat ctgatttatc gtcaaccgcg
     6781 tgcttcaaac cagtcacaaa atcttctgct gttactgggg catattcttc accatcagcc
     6841 gtataccaag agacaccatc gcgaagagta taagtataag tcagaccgtc ttttgaaacc
     6901 ttccaatctt tagcaagtga tggtactaaa ttaccatact catcgttttc caaaagacca
     6961 tcaaccattt gtgttatcac atcagtcgtt ccaccgccat tagtcaaaat ataatccaat
     7021 gattttggat cgttaacaaa aacgtacttg taggtttttg tagcttctga gccacctgaa
     7081 gcatttttat taccacaagc tgccaaagcg gatactgaca agatcgcaac acttacagct
     7141 gccaaccatt tacttttctt cataaaagtc acccctaaac tttatttttc attttttggc
     7201 aattgctaaa tgaaattggt taattgaaga cattatatca taaaactttc ttaaagaaaa
     7261 taatgaaatg aaaaattatc tgaatatttc gtagaatttt ctttatt
//
LOCUS       DATURV010000066         7212 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DATURV010000066 DATURV010000000
VERSION     DATURV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.066"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..835
                     /gene="dnaN"
                     /locus_tag="VCF75_001415"
     CDS             <1..835
                     /gene="dnaN"
                     /locus_tag="VCF75_001415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEP2546968.1"
                     /translation="HQVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSI
                     IAETAFAASLQESRPILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVI
                     PSKSLREFSAVFTDDIETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFE
                     TEVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVS
                     QSGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRT
                     N"
     gene            910..1107
                     /locus_tag="VCF75_001416"
     CDS             910..1107
                     /locus_tag="VCF75_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEP2546969.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1437..2552
                     /gene="ychF"
                     /locus_tag="VCF75_001417"
     CDS             1437..2552
                     /gene="ychF"
                     /locus_tag="VCF75_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEP2546970.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2622..3191
                     /locus_tag="VCF75_001418"
     CDS             2622..3191
                     /locus_tag="VCF75_001418"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEP2546971.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            3194..6697
                     /gene="mfd"
                     /locus_tag="VCF75_001419"
     CDS             3194..6697
                     /gene="mfd"
                     /locus_tag="VCF75_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEP2546972.1"
                     /translation="MNILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GISINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLVVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFTDNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYERLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEHEIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQISSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVTRQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVAELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYISDERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKNNSVFK"
     gene            6859..7131
                     /locus_tag="VCF75_001420"
     CDS             6859..7131
                     /locus_tag="VCF75_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEP2546973.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
     gene            7118..>7212
                     /locus_tag="VCF75_001421"
     CDS             7118..>7212
                     /locus_tag="VCF75_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEP2546974.1"
                     /translation="MKKPSIVQLNNHYIKKENLKKKFEEEESQKR"
BASE COUNT         2500 a         1083 c         1395 g         2234 t
ORIGIN      
        1 acaccaagtt gttttaacca gtggtaaatc agagattacc ttaaaaggaa aagatgttga
       61 ccagtatcct cgtctacaag aagtatcaac cgaaaatcct ttgattttaa aaacaaaatt
      121 attgaagtct attattgctg aaacagcttt tgcagccagt ttacaagaaa gtcgtcctat
      181 tttaacagga gttcatattg tattaagcaa tcataaagat tttaaagcag tagcgactga
      241 ctctcatcgt atgagccaac gtttaatcac tttggacaat acttcagcag attttgatgt
      301 ggttattcca agtaaatctt tgagagaatt ttcagcagta tttacagatg atattgagac
      361 cgttgaggta tttttctcac caagccaaat cttgttcaga agtgaacaca tttcttttta
      421 tacacgcctc ttagaaggaa attatcccga tacagaccgt ttattaatga cagaatttga
      481 gacggaggtt gttttcaata cccaatccct tcgccacgct atggaacgtg ccttcttgat
      541 ttctaatgct actcaaaatg gtactgttaa gcttgagatt actcaaaatc atatttcagc
      601 tcatgttaac tcacctgagg ttggtaaggt aaacgaggat ttggatattg ttagtcagtc
      661 tggtagtgat ttaactatca gcttcaatcc aacttacctt attgagtctt taaaagctat
      721 taaaagtgaa acagtaaaaa ttcatttctt atcaccagtt cgaccattca ccctaacacc
      781 aggcgatgag gaagaaagtt ttatccaatt aattacacca gtacgaacaa actaagtaag
      841 aaaaagctcc cttttaggag tttttttgtt attataaata ttaacgataa tataagtgga
      901 gaaaagggaa tgtatcaaat tggatcattt gttgaaatga aaaaacctca tgcttgtgta
      961 attaaagaaa ctggtaaaaa ggctaatcaa tggaaggtac tgagagtagg agctgatatt
     1021 aaaattcagt gtactaactg tcagcatgtg attatgatga gtcgttatga ttttgagcga
     1081 aaattaaaaa aagtactgca accatgaaag tccttttaaa taaataattc tagctatatt
     1141 tgcaaccaat actttcctaa aaaattgcta gtatgccgtt ggaaaattcg ctattctaat
     1201 gttatcgaaa gaagaaagat ggccattgac aatgacgaaa gttgtagcac agttacttag
     1261 tttctcggca aactatatca aaatgagaat aatacaccaa actttgataa tttaaatgaa
     1321 ggtgaaagag tggcataatg ataagcaaat ctgaaatttt cttcttattc ataccgcttt
     1381 ttcttctatt ttttggtata ataatcttga ttgaaatttg aatggagatt cgctaaatgg
     1441 ctttaacagc aggtattgta ggcttaccaa atgttggtaa atcaacctta tttaacgcaa
     1501 ttacaaaagc aggagcagaa gctgctaatt atccttttgc gactattgat cccaatgttg
     1561 gtatggtaga ggttccagat gagcgtctgc aaaaattgac agaattgatt acacctaaaa
     1621 aaacagttcc gacaaccttt gaatttactg atattgcagg tatcgttaaa ggtgcttcta
     1681 gaggggaagg tctaggaaat aaatttttag ctaatattcg tgaagtcgat gctattgttc
     1741 atgtggtacg tgcttttgat gatgaaaatg tcatgcgtga acaaggtcgt gaggatgctt
     1801 tcgttgatcc tatagcagat attgacacta ttaatcttga attaatctta gctgatttag
     1861 agtcaatcaa taaacgttat gcgcgtgttg aaaaaatggc acgaactcaa aaagataaag
     1921 aatcagtagc agagtttaat gttttgcaaa agattaaacc tgttttggaa gatgggaaat
     1981 cagctaggac aattgagttt acagaagatg aagcaaaagt tgttaaaggt ctctttttat
     2041 taacaactaa acctgtttta tatgtagcta atgtcgatga agataaagtt gctaatccag
     2101 atggtattga ttatgtcaaa caaattcgtg actttgcagc tactgaaaat gctgaagtag
     2161 ttgttatctc agcgcgtgca gaagaagaaa tttcagagct tgacgatgag gataaagaag
     2221 aatttttgga agctatcggt cttactgaat caggcgttga taaattaacc agagcagctt
     2281 atcatctctt aggccttgga acctatttta cagcaggtga aaaagaggtt cgtgcttgga
     2341 cgtttaagcg tggtataaaa gctccacaag ctgctggtat tatccattca gattttgaaa
     2401 gaggttttat tcgtgcagta accatgtctt atgatgatct aatgacctac ggttcagaaa
     2461 aagccgtcaa agaagctgga cgcttgcgtg aagaaggaaa agaatatgtt gtccaagatg
     2521 gggacatcat ggaattcaga tttaatgtgt aattatatta aaataatatc agaaggttgg
     2581 aagaacattc cagccctttt ggcattttag aaagagaaaa tatggtaaaa atgattgttg
     2641 gtctgggaaa tccaggctct aaatatgaaa aaacaaagca caatattggt tttatggcta
     2701 ttgacaatat tgtcaagaac cttgacgtta cctttacaga tgataaaaat tttaaagcac
     2761 agataggaag tacttttatt aatcatgaaa aagtttactt tgtgaaacct actactttta
     2821 tgaataatag cggcatagca gtaaaagcat tactaaccta ctataatatt gacataacag
     2881 atttaattgt tatctatgat gatttagaca tggaagtcag taaattacgt ttacgtagta
     2941 agggttcagc aggaggacat aatggcatta agtcaatcat tgcccacatt ggaactcagg
     3001 aatttaaccg aatcaaagtt ggtattggac gacctttaaa aggtatgact gttattaacc
     3061 atgtgatggg ccaattcaat accgaagata atattgctat ttcgttaact cttgacagag
     3121 ttgtcaatgc tgtcaagttt tatttacaag aaaatgattt tgaaaaaaca atgcagaaat
     3181 ttaatggata atcatgaata ttttagaatt atttagtcag aataagaaag tccaatcctg
     3241 gcactctgga ttaaccacct taggaagaca actggtaatg gggttatcgg gttcaagtaa
     3301 agcattggct atagcttccg cttatttaga tgatcaaaaa aaaatagttg tggttacatc
     3361 aactcaaaat gaggttgaaa aattagccag cgatttatct agtttacttg atgaagaact
     3421 tgttttccaa ttttttgcag acgatgtggc tgcagcggaa tttatctttg cgtcaatgga
     3481 taaagctcta tcaagaatag aaaccctgca atttttaagg aatcctaaat ctcagggcgt
     3541 tttaattgtt agtttatcag gcttaagaac tttattgcca aacccagatg tttttacaaa
     3601 gagtcagatt caactaacag ttggagaaga ttatgatagt gatactctta ctaaacaact
     3661 gatgacaatt ggatatcaga aggtctcaca ggttattagt ccgggagaat ttagccgtcg
     3721 aggggatatt ttagatatct atgagattac acaagaattg ccttatcgat tggaattttt
     3781 tggtgatgat attgatagta ttaggcaatt tcatccagaa actcaaaaat cttttgaaca
     3841 actagaaggt atttctatta atccagcaag tgatcttatt tttgaggcta gtgattttca
     3901 acgtggcatt gagcaattag aaaaggctct acaaacagca caagatgata aaaaatctta
     3961 tttagaagat gtattagttg tttcaaaaaa cggttttaaa cataaggata tccgtaaatt
     4021 tcaatcatta ttttatgaaa aagagtggtc attattagat tatattccta agggaacgcc
     4081 aatctttttt gatgattttc aaaaactagt tgataaaaat gcaagatttg atttagagat
     4141 tgctaatctc ttgacagaag atttacagca aggaaaggct ctttccaatc ttaactactt
     4201 tacagataat tatcgagagc ttaggcacta taagccagcg accttctttt caaattttca
     4261 taagggactt ggaaatatca aatttgatca gatgcatcag ctaactcagt atgccatgca
     4321 ggaatttttt aatcaatttc ctttgttgat tgatgagatt aaacggtatc aaaaaaatca
     4381 aacaacggtt attgtacagg tagagtctca gtatgcttac gaacgacttg aaaaatcttt
     4441 tcaagattac caatttcgtc ttcctttagt gagtgctaat caaattgttt cacgtgaatc
     4501 acaaattgta attggagcta tctccagtgg tttttatttt gctgatgaaa agttagcgtt
     4561 aatcacagag catgaaattt atcataaaaa gatcaaacga cgcgctagac gatctaatat
     4621 tagcaatgct gagcgtttga aagattacaa tgagttagca gtaggtgatt acgtggttca
     4681 taatgtacat ggtatcggtc gctttcttgg aattgaaacg attcagattc agggaatcca
     4741 tcgagattat gttactattc aatatcaaaa ttcagaccgt atttctcttc caattgacca
     4801 aattagtagc ttatcaaagt acgtttctgc tgatggaaaa gaacctaaaa ttaataaact
     4861 caatgatggt cgtttccaaa agacaaagca aaaggtcact agacaagtag aggatattgc
     4921 tgatgacctt ctaaaattat atgctgaaag aagtcagcaa aaaggatttt cattttcacc
     4981 agatgatgac ttgcagcgcg cttttgatga tgattttgct tttgtagaaa cagaagatca
     5041 acttaggtct ataaaggaaa ttaaagctga tatggagagc atgcagccta tggatcgtct
     5101 tttagtaggc gatgtaggat ttggtaagac agaagtagct atgagggcag cctttaaagc
     5161 ggtgaatgat cacaaacaag tagctgtctt agttccaacc acagtcttgg cccagcagca
     5221 ttatgaaaac ttcaaagcac gctttgaaaa ttaccctgtt gaggttgacg tcttaagtcg
     5281 tttccgtagt aaaaaagagc aagctgaaac actagaacgt gtacgaaaag gtcaaattga
     5341 cattattatc ggaacccatc gactcctatc aaaagatgtg gtcttttctg atttaggatt
     5401 gattgtgatt gatgaggaac aacggtttgg tgttaagcat aaagaaactt taaaggaatt
     5461 aaaaactaag gttgacgtct taaccttaac agctactccg attcctagaa ccttacacat
     5521 gtctatgtta ggtatccgag atttatcggt tattgagacc ccaccaacca atcgttatcc
     5581 tgttcaaacc tatgttttgg aaaataatcc aggtctcgtt agagaagcta tcattcgtga
     5641 aatggatcgt ggaggacaaa ttttttacgt ttacaataaa gttgacacta ttgaaaagaa
     5701 agttgcagag ctacaagaat tagtcccaga agcttctata ggttttgttc atgggcaaat
     5761 gagtgaaatt caacttgaaa ataccttgat tgactttata aatggtgatt atgatgtcct
     5821 tgtggcaaca acaatcatcg aaacaggagt tgacatttct aatgtaaaca ctttgtttat
     5881 tgagaatgct gatcatatgg gattgtcaac tttatatcaa ctacgagggc gcgtcggaag
     5941 aagtaatcgt attgcttatg cttacctgat gtatcgtcct gataaggtcc taacagaagt
     6001 ctctgaaaag cgtttagagg ctattaaagg ctttactgaa ttaggttcag gcttcaagat
     6061 tgctatgcga gatttgtcta tacgaggagc aggtaatatt ttaggggctt ctcagagtgg
     6121 ctttattgat tcggtcggtt ttgaaatgta ttctcagtta ttggaacagg ctattgctag
     6181 caagcaagga aaaacgactg ttcgccaaaa aggtaatact gaaatcaatc ttcagattga
     6241 cgcctatttg ccagatgatt acatatcaga tgagcgtcaa aaaattgaca tttacaagcg
     6301 tattcgagaa attcaatcaa gagaagatta tctcaatttg caggatgagc tgatagatcg
     6361 ttttggagag tatcctgatc aagttgccta tttgttagag atagctctgc taaaacatta
     6421 tatggacaat gcctttgcgg aattagttga acgcaaaaat aatcaggtca ttgttcgatt
     6481 tgaagtaact tctttaactt atttcttgac tcaggattat ttcgaagcct tatcgaaaac
     6541 ccatcttaaa gctaaaatta gtgaacatca ggggaaaatc gatatcgtct ttgacgttcg
     6601 ccatcaaaaa gattatagaa ttttagaaga attgatgtta tttggagaaa ggcttagtga
     6661 gataaaaatc agaaaaaaca attcagtttt taaataataa tttgaaacaa cttttagact
     6721 gaatagtatt aatgacaatg gaacgaacta gctctttata cttttgtcaa aaatctaaga
     6781 tctttgctgc gaaattagga tcttttctac catttaaaaa ataaaaatga taaaatgaag
     6841 aggattgtag gagaaattat gagactagat aaatatctaa aggtatcgcg ccttattaaa
     6901 cgtcgttcag tagcaaaaga agttgcggat aagggacgaa ttaaagttaa tgggatactt
     6961 gctaaaagtt caacgaatat aaaactaaat gatcacattg aaattagttt tggaaataaa
     7021 ttactgacag taagagtcat tgaaataaaa gatagtacaa aaaaagaaga tgctcttaag
     7081 atgtatgaga taatcagtga aacaaggata acattaaatg aagaagccta gtattgttca
     7141 attaaataac cattatatta agaaagagaa tctcaaaaaa aaatttgaag aagaagaatc
     7201 tcaaaaaaga aa
//
LOCUS       DATURV010000067         7210 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DATURV010000067 DATURV010000000
VERSION     DATURV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.067"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            245..1480
                     /gene="arcA"
                     /locus_tag="VCF75_001422"
     CDS             245..1480
                     /gene="arcA"
                     /locus_tag="VCF75_001422"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEP2546975.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            1580..2008
                     /locus_tag="VCF75_001423"
     CDS             1580..2008
                     /locus_tag="VCF75_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2546976.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQSYGPKCLEDG
                     EYCQSLAKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNN"
     gene            2048..3061
                     /gene="argF"
                     /locus_tag="VCF75_001424"
     CDS             2048..3061
                     /gene="argF"
                     /locus_tag="VCF75_001424"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEP2546977.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3231..4724
                     /locus_tag="VCF75_001425"
     CDS             3231..4724
                     /locus_tag="VCF75_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEP2546978.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            4741..6072
                     /locus_tag="VCF75_001426"
     CDS             4741..6072
                     /locus_tag="VCF75_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEP2546979.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPKGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTEPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYQG
                     DKLEALQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAAQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            6092..7042
                     /gene="arcC"
                     /locus_tag="VCF75_001427"
     CDS             6092..7042
                     /gene="arcC"
                     /locus_tag="VCF75_001427"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEP2546980.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALISTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKTLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYVNFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
BASE COUNT         2180 a         1360 c         1521 g         2149 t
ORIGIN      
        1 gtttttctga cttattttaa tttataagta taaattttta cactaagatg taaaaaacat
       61 acaagtaaac gaatacaaat ctataaaaat aaaaacaacg ctcaaaaaat gtaagcgatt
      121 actcaaaata gctgtgagaa ataccctatt tttatctttt ttaaggagta cactaggaat
      181 ataagcaaga gttaagaatc ttgatggtga attcacaaaa accaattaga aagaggtaat
      241 tcttatgact gctcaaacac caattcatgt ttattctgaa attggaaaat taaaaaaagt
      301 gttgctccac agacccggta aagaaattga aaatcttatg ccagactact tggaacgctt
      361 gttgtttgat gatattccat tcttagagga tgcccaaaaa gaacatgatg cttttgccca
      421 agctttgcgt gacgaaggta ttgaagtctt gtaccttgag accttagctg cagaatctct
      481 tgtcactcca gaaattcgcg aagctttcat cgatgaatac ctaagtgaag ctaatatccg
      541 tggaagagct actaaaaaag ccattcgtga attgttgatg gctatcgaag acaatcaaga
      601 attgattgaa aaaaccatgg caggagttca aaaatctgaa cttccagaga tcccagcatc
      661 tgaaaaaggc ttgactgact tggttgaatc caattaccca tttgccatcg acccaatgcc
      721 aaacctttat ttcacacggg acccattcgc aactatcggt acaggtgttt ctcttaacca
      781 catgttctca gaaacccgta accgtgaaac cctttatggt aaatacattt tcactcatca
      841 cccaatttat ggtggtggca aagtgcctat ggtttatgac cgtaatgaaa ccactcgcat
      901 tgaaggtggg gacgaacttg ttctttcaaa agatgtgctt gcggttggta tttctcaacg
      961 tacagatgct gcttctattg aaaaattgtt ggttaacatc tttaaacaaa accttggctt
     1021 caagaaagta ttggcctttg aatttgcaaa taaccgtaaa tttatgcact tagacactgt
     1081 ctttaccatg gttgactatg acaaattcac cattcaccca gaaattgaag gcgatcttcg
     1141 tgtttactct gtcacttacg acaatgaaga acttcatatt gttgaagaaa aaggtgattt
     1201 agcagaactt cttgctgcta accttggtgt tgaaaaagtt gaccttatcc gttgtggtgg
     1261 tgacaactta gtagcagcag gtcgtgaaca atggaacgat ggttctaata ccttgaccat
     1321 cgcaccaggt gtggttgtgg tttataaccg taataccatt accaatgcca ttcttgaatc
     1381 taaaggcttg aaattgatca agattcacgg aagtgaattg gttcgcggtc gtggtggacc
     1441 tcgttgtatg tcaatgccat ttgaacgtga agatatttaa taagctatgg taaaggtggt
     1501 tgtaggtcag aagccttttt aaagggcagc tagtgtttat cttagcttct gtgtctttgt
     1561 ccttcaatga aattttgtca tgacagataa atttgatgcc aatgacgaaa caagaacggt
     1621 ttatgcagtc gtttatgaca atgaccagcc cgtttcaaca ggacaatttt tagctgaaac
     1681 gaaaatagaa gcacgattga cacgcattgt aaccttagca gattattgtg gttgcggtta
     1741 tggtgccaaa gtcactgaag cgctagaaac ttataccaga cgagaaggct tttaccaact
     1801 aaccattcac agtgaactga cagcacaaac cttttatgaa aacctaggtt atcagtccta
     1861 tggtcccaag tgtttagaag atggtgagta ttgtcagtcc cttgcaaaaa ccattcttaa
     1921 atgggagaag aatatggaca tagcaatgct aattgcgatt gttggtggtc tattaggctg
     1981 ctatctctat ctcacaaaaa ataattaatc aaaacagatt acagttaaca ctcgaaggag
     2041 acaatagatg acacaagtat ttcaaggacg tagcttccta gcagaaaaag attttacacg
     2101 cgctgaatta gaatacctta ttgatttttc agcacatttg aaagatttga aaaaacgtgg
     2161 tgtgcctcat cactacttag aaggtaaaaa cattgccctc ttgtttgaaa aaacatcaac
     2221 tcgtacgcgt gcagctttta caacagcagc cattgaccta ggtgctcacc cagaatacct
     2281 cggtgccaat gacatccaac ttggtaaaaa agaatcaaca gaagacactg ctaaagtatt
     2341 gggtcgtatg tttgatggga ttgaattccg tggctttagc caacgcatgg ttgaagaatt
     2401 ggcagaattc tcaggtgtgc cagtttggaa tggtttgact gatgaatggc acccaaccca
     2461 aatgcttgct gactatttaa cagtgaaaga aaactttggc aaacttgaag gccttaccct
     2521 tgtttactgt ggtgatgggc gtaataacgt tgccaattcc cttcttgtga ctggtgctat
     2581 ccttggtgtt aatgttcata tcttctcacc aaaagaactc ttcccagaag aagagattgt
     2641 gacattggct gaaggctatg ctaaagaaag tggcgctcgt atccttatca cagaagatgc
     2701 tgacgaagct gttaaaggcg cagacgtcct ttacacagac gtttgggtat caatgggtga
     2761 agaagacaaa ttcaaagaac gtgttgaatt gcttcaacca tatcaagtca acatggacct
     2821 tgttcaaaaa gcaggaaatg acaagcttat cttcttgcac tgcttgccag ctttccatga
     2881 cacaaacacc gtttatggta aagacgttgc tgaaaaattc ggcgtgaaag aaatggaagt
     2941 cactgatgaa gtcttccgca gcaaatacgc tcgtcatttt gatcaagctg aaaaccgtat
     3001 gcacactatc aaagcggtta tggcagcaac ccttggtaac ctctttattc caaaagtgta
     3061 ataaggactg gtactcctta actcatttca catatcaatg tgatcccttt aatttaacaa
     3121 tttaactact taaaaacgta ctttaactac atgacaagtc gttctggttt tcgcgtcctt
     3181 gaccggaact agaacggctt atttttatga aaaggtaaga ggtaatcatt atgacagaag
     3241 aaaaaaaacg gggttttaga attccttctt cttacaccgt tctttttatc attatagcca
     3301 tcatggcagt gttaacttgg tttattccag ctggcgccta cgaaaccgct aagggcggtg
     3361 gtgttatttc aggaacctac aaaacagtag catccaatcc tcaaggattt tttgacatct
     3421 tgatggctcc cgttcgaggc atgttaggtg ttgaaggcac tgatggtgct attcaagtgt
     3481 ctttctttat cttaatggtt gggggctttt taggagttgt caacaaaaca ggtgctcttg
     3541 acacaggaat tgcgtcagtg gttcgtaaaa acaaaggtag agaaaaaatg ctgattgcta
     3601 ttttaatccc tttgtttgct ctaggtggaa cgacctatgg tatgggggaa gaaaccatgg
     3661 ccttttaccc acttttaatt cctgttatga ttgcggttgg ttttgacagt attgttgctg
     3721 tagccattat tttgattggt tctcaaattg gatgtctagc ctccactatt aacccatttg
     3781 caactggtgt agcagctgat gctgctggtg tcagcattgc agatggaatg atttggcgtg
     3841 ttatccaatg ggttattctt gtcggcatgt ctatttggtt tgtttacaat tacgctagta
     3901 agattgaaga agacccaagt aaatcattgg ttgcagataa ggaagaagag cataaggaac
     3961 tcttccagtt gcaaaattct ggagaagact taaacaaacg ccaacgaaac gttttgacca
     4021 tttttacttt gacctttgtc attatgattc ttagtttgat tccgtgggaa gattttggta
     4081 tcaaattctt tactaatatc aatacttggt taaccacaat gcctatctta gggggagtta
     4141 ttgggaaaac catgggagca tttggtactt ggtatttccc agaaatcacc atgctcttta
     4201 tcatgatggg tgtcttagtt gctattgttt atcgtatgag tgaagaagac ttttttagct
     4261 cttttttgac tggtgcaggt gaatttttag gtgttgccat gatctgtgct attgcacgcg
     4321 gtattcaagt tatcatgaat ggtggtatga ttacagccac tatcttacac ttaggtgaaa
     4381 caagtctttc tggtttatct tctcaagttt ttgtgatatt agcttatatt ttctacctcc
     4441 caatgtcctt cttgattcca tcaacatcag gacttgctgg ggctacaatg ggaattatgg
     4501 caccgcttgg acaattctca aatgtccctg ctcaccttgt tattacagcc ttccagtcag
     4561 cttctggaat cttaaacatg atttctccaa cttcagcaat cgttatggga gcacttgcgc
     4621 ttggtcgcgt tgaccttggt acttggtgga aattcattgg taaatttatt gtaatggtga
     4681 tgcttgtaag cgtgctatta cttgtagttg caacattctt ttaacagata ggaggttcct
     4741 atggaatcct atatcacacc taaacatcaa gaggcttgtg tagctgccat taaacagatc
     4801 gtttcttacc cttctgtttg tcatgaagga gaaaatggca caccttttgg acaggccatt
     4861 caagatgttc tcgaagcaac cttagactta tgtcaaggtc ttggcttcca tacttatatt
     4921 gatcctaaag gttactatgg ttatgctgag ttaggtgacc aaaaagaggt cttggctatt
     4981 ctgtgccatt tggacgttgt tccagaagga gatcgtcagt tatggaagac agagcctttt
     5041 gactgtgttg aggcagacgg ctgtttattt ggtcgaggca ctcaagacga taaagggcct
     5101 actatgatgg cactatttgc caccaaagcc ttgatagatg caggcgttac ctttaataaa
     5161 cgcattcgtt ttattttcgg aacagatgaa gaaactttgt ggcggtgtat gaatcgctac
     5221 aatgatgtag aagaacaggc tacctttggg tttgctcctg attcaagttt ccctttaacc
     5281 tatgccgaaa aaggcctact tcaggcaaaa ttagtgggga aaggttctga taaactgtca
     5341 cttgaaatcg gacaagccta caatgttgtt cctgcaagag cctcttatca aggtgataag
     5401 cttgaggctt tacaaaaaga actagataag cttggctttg agtacgtcgt caaagaggga
     5461 gaattaacgg tttatggtct cgcccaacat gctaaagatg ctcccgatgg catcaacgct
     5521 atgattaggc ttgccaaagc tttagtggtc cttgaaccta acaaaaccct tgattttctt
     5581 gctaatgtca ttgatgaaga tggcagagca cttaatattt ttggcagaat cgaagatgaa
     5641 ccttcaggca aattaagctt taatgccgca gggcttacct taaccaaaga caaggcagaa
     5701 attcgcctag atattcggat tccagttttg gcagataagg aaaaattagt ccaacaatta
     5761 agccaaaaag cacaagaata cggcctaacc tatgaagaat ttgattattt ggctccatta
     5821 tatgtgccat tagacagtga attggtaaca accttgttat ctgtctatcg gaaaaaaaca
     5881 ggtgatcaaa gccctgccca atcatcagga ggggcaacct ttgctagaac aatgaagaat
     5941 tgtgttgcct ttggtgccct tttccctgat gctgctcaaa cggaacacca agaaaatgaa
     6001 catattgttt tagcggatgc ttaccgcgct atggatattt atgcggaagc tatttaccga
     6061 ctaacgcgat aacaagatat gaggagtaat tatgacgaaa caaaaaatcg tagtcgcact
     6121 tggaggaaat gctattcttt caacagatgc gtcagcaaaa gcacaacaag aagccttgat
     6181 ttccacatca aaatcattgg ttaaattaat taaagaaggt catgaagtca tcgtgaccca
     6241 cggcaatggc ccacaagttg gtaatctctt attacaacaa gcagcggcag attctgaaaa
     6301 gaatccggct atgcctttgg atacttgtgt tgccatgaca gaaggtagca tcggtttttg
     6361 gttagttaat gcccttgata atgaattgca agcccaaggt attcaaaaag aagtggcagc
     6421 tgttgtcact caagtgatcg tggatgccaa agacccagct tttgaaaacc caacaaaacc
     6481 aattggtcca tttcttaccg aagaagatgc caaaaaacaa atggcggagt ctggtgcaag
     6541 tttcaaagaa gatgcaggtc gtggctggcg taaggttgtg ccatcaccaa aaccagttgg
     6601 tattaaagaa gccaatgtca tcagaagtct cgttgattct ggcgttgttg tggttagtgc
     6661 tggtggtggc ggtgtgcccg ttgtggaaga tgctactagc aagaccctga ctggtgtgga
     6721 agctgttatt gacaaggact ttgctagtca aaccctttca gaattggtgg acgcagacct
     6781 ctttatcgtt ttgacaggag ttgataatgt ttacgtcaac ttcaacaaac ctgaccaagc
     6841 caaattggaa gaagtgactg tctctcaaat gaaagaatac atcacacaag accaatttgc
     6901 accaggtagt atgttaccaa aagtggaagc tgccattgct tttgttgaaa acaaaccaaa
     6961 tgccaaagcc attatcacat cgctagaaaa tattgacaat gtactatcag caaacgctgg
     7021 aacacaaatt atcgcagggt aatcaagagg atttttagtt ctgtttaaaa gactcattta
     7081 atggtatttt ctgaagagta cgactgacat aaagagttga tactgttttt aaaggaaaag
     7141 agtgtgccat aataaaaaac aataatagta ttagaaaagg ctaattttat tgagaattag
     7201 ccttttcttt
//
LOCUS       DATURV010000068         7134 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DATURV010000068 DATURV010000000
VERSION     DATURV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.068"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            118..279
                     /gene="sagA"
                     /locus_tag="VCF75_001428"
     CDS             118..279
                     /gene="sagA"
                     /locus_tag="VCF75_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEP2546981.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            501..1451
                     /gene="sagB"
                     /locus_tag="VCF75_001429"
     CDS             501..1451
                     /gene="sagB"
                     /locus_tag="VCF75_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEP2546982.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1448..2506
                     /locus_tag="VCF75_001430"
     CDS             1448..2506
                     /locus_tag="VCF75_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEP2546983.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAINTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSDALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHACTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2526..3884
                     /locus_tag="VCF75_001431"
     CDS             2526..3884
                     /locus_tag="VCF75_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEP2546984.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3859..4530
                     /locus_tag="VCF75_001432"
     CDS             3859..4530
                     /locus_tag="VCF75_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP2546985.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELIFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4527..5210
                     /locus_tag="VCF75_001433"
     CDS             4527..5210
                     /locus_tag="VCF75_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEP2546986.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLMALSYQDFLDV
                     LLSLFQFVVIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5233..6156
                     /locus_tag="VCF75_001434"
     CDS             5233..6156
                     /locus_tag="VCF75_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2546987.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6165..>7134
                     /locus_tag="VCF75_001435"
     CDS             6165..>7134
                     /locus_tag="VCF75_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2546988.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIRLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLS"
BASE COUNT         2219 a         1101 c         1378 g         2436 t
ORIGIN      
        1 caacagtaca attgagctag ccttgtcctt gttgtgttaa ctttattttt aaaataaggt
       61 taaaaataaa cgactcgcgt tcttatcagt tacttattag ataaggaggt aaaccttatg
      121 ttaaaattta cttcaaatat tttagctact agtgtagctg aaacaactca agttgctcct
      181 ggaggctgct gttgctgctg tactacttgt tgcttctcaa ttgctactgg aagtggtaat
      241 tctcaaggtg gtagcggaag ttatacgcca ggtaaataat ctatttagca tctctatgtg
      301 atagtgatat taaggtaatg agtttgctag caactaagtt atctttggta acttctgatt
      361 atagatgaat ggcatagagg tgttagaaaa catgagacaa aagtaatctt cttgatgcta
      421 acgtctcagg taattagcag gtactagata gtacctgcta attactatat gtttagtaaa
      481 atgagatagg aaagatagtt atgtcatttt ttacaaagga acaacaacct aaagagaatt
      541 gtccgccaat aactgttgaa aaagcaaggc aattgtttga atttaataca aaccacttgt
      601 ccttatcgga ttaccatcat caaacggtgc taaaaacgtc aaagcagcta gttgctcaac
      661 atttaatgcc taatgcaacc gataatctta gtcaacattt tttgatgaac tataaagcta
      721 ataataatta tttaggcttc caagctagta ttgtcgactt ttttacagat tctgccgttg
      781 ctaatttttc aagtagttac gtttatgaaa gtcaggaaaa gataattcgt ttaccaaaac
      841 ctaccaagat atcaactgct ctgtcgacat gtattataaa acgaagaagt catcgtcaat
      901 tttcagatag acaaatgcct cttcaagatt tatcaaacat tctttattat gcatgtggtg
      961 ttagttcaca agcatcaatt agagatggag catcagataa gattacactc agaaactgtg
     1021 cttcaggtgg aggtttatac cctattcatt tagtttttta tgctagaaac atcagtaaat
     1081 taatagatgg tttctatgaa tacctaccct atcagcatgc actaaggtgt tatcggcata
     1141 gctctgagga aaacgttaga gattttgcgg aatacggtgc tattaatgct gaaaattgta
     1201 atattattat tatttatgtc taccattaca tgaaaaatac acgtaaatat gggaatcagg
     1261 cgactgccta tgcttttatt gaatcaggag aaatagccca gaatattcaa ttgactgcaa
     1321 ctgccttaac ttatggaagt attgatattg gtggttataa taaggaatat ctccaagaat
     1381 tattagattt agatgggcta ggagagcatg tgattcacat gacactcgta ggaactaagg
     1441 agtctcaatg aaatatcaac ttaatagtaa tgttcgtgtc gtgacatttc aagatacatt
     1501 ttgttttaga aaaggaatct gggactttaa tgaagccatt ctagatttga cacaagaacc
     1561 tcaaaattta aagatagttt atcaagaaat agtttcacag ttggttaagg gagtagccat
     1621 taatactgaa acttatgaaa atactttaga acctgagacg ttcgctaatt taatggaagt
     1681 catttcaggc ttgtattaca atgatatgct gatgcttgag gatgactaca atcttgaaga
     1741 aaatgtcatg aaaattttaa tgggtaattt ccgttttatg gcgcaagcag gtcatatggt
     1801 aagcaatgat cctgttttgt ttatcagtga ttcgacttat gttaatgaat ctgctgagtt
     1861 gctagctgaa caactgcatt tacaattgca agtggctagc gatgacttaa aaatgttaat
     1921 tcagcagaca gatgttagtt cgcgtttaga tgctttggag catcgccgta acatgaattg
     1981 tttatcagat gccttgtgcg attaccaaag tattattatt tgtcaagagc gcttgaatat
     2041 tatgatgttg cgacacttga atgaagttag cgtagctatg aaaaaacaat tagtgattgg
     2101 ttttgtagat ggaccattct tgcacgcttg tactttgaat cctcctcata gtgctgactt
     2161 tgatagctta gaaagaagag ttttggcacg tttacaagac tcaactctct atcaacattt
     2221 tgctaatcag attttaccag caacacaaga tgtgagccag gcctatttac cattgttaaa
     2281 tgttttaatg aatttagttg ttagcgaagc tttcattatt gctcaaacag gtagttctaa
     2341 atttgaggga cgtttattaa gcatttactt accgacgctt gagattcagg tccaagatat
     2401 tttgaaaatg tctaattcac agacacaagg agctttggca aaattaaagt atgaggatca
     2461 gcagatatca acacgtgaga ttgttaaaaa actccttgac gagtaaccta taaggagaat
     2521 ctattatgtt atactattat ccttctttta atcatatttt tgatgaatta aaaagtttaa
     2581 gtggcaatcg aacaggtatc ttaaatcaat ctcaagttcc agtttgcaac catcctcacg
     2641 atgtttactt aaaaagtatt actggccaaa tgccagatta tcataaacaa tttataggtg
     2701 agttgagtca agttagttat cacattattg gttatggcag ttactatgaa gaagcactta
     2761 tcaagtattt aggagaaagt attgaacgct atgctactgt aattgccgga gacttactat
     2821 cagatcgtat tgtctatgct tcttataatg agttaaaact gttacataaa gtgatgcctt
     2881 tagaatattt gcaagtgttt acccaagaac agatcgcatt atcttgtgac ttgcaaatga
     2941 tgatgtgcga taaaatggta actgagaacg atgtattggg ctgggttaaa tgtcccatgt
     3001 tctttgagga cgcagagatg tatgttccag ctcaaatgct ttgtgttggg tataaaacaa
     3061 atgagactgt aggagaaaga cggataatac caggattttc tacgggaaca gcttctcaca
     3121 aaacgttaga agcggctatg tgcaacagtt tgattgaata catccagatc gattcaatga
     3181 tgcttagctg gtatactaag aagccttgcc ccaagattat tgttgatgat cctgatattg
     3241 aagtgatttt agaagaagct agactgggaa aagacagtct atacgacatt attcctattg
     3301 atatgactgt aggagaagat aatcctcttt atacgtttgg aatcattctc aaaaataaat
     3361 atgatgaagg gccttacctc ttgtttggag tgcaggctgg gttagatcct aaacatgctt
     3421 tgctaagagg gatcatggaa gcttcagcta ttagctatag ttactactat aatcttttat
     3481 atcaaaaagc ttcccttgca aatattgaat gcgaagaacc tttgttttta gatttagata
     3541 gtaatgtctt ctattatgca catcccaagg accaagacca taaatggaag gcctttgaac
     3601 ctttgatttc aggagaagtt ctgttaagtg atttggaaga tcattctggt aaagataaaa
     3661 aagaagattt gaagacttta ttagcttatg ctaagaaagt cagtcctaat gctgtctttt
     3721 tagatattac acctcctgaa gctctagaaa aaggctggta cgtcacgcga gttctgatgc
     3781 cagaacttct tgaaatgtgt ataccagcat ttccttttgc taatcatcca cgaatgagac
     3841 agtttggagg tgtaacaaat gcctttgtcc atccaatgcc ttaatctttg ttttttgttg
     3901 gtaacttttt gtccttcaat tcctatgcaa gctatttttg gaaaggaaga tagtggttat
     3961 gcatttaatt taattggttt tctaagagca actctgattt atgacatttt ggctttggta
     4021 agtatttatg ttttgtcacc tcaaataacc ttgtctcttg aaagtattga tagtaagacg
     4081 ttctttatgg gacttgtatt ttgcgtcttg atagtactga ttgaactcat tttcttacat
     4141 ggtttacgtt gttggcaaaa aaagcaatgg cttcctgcaa ctttctcgtt tgtaggaacg
     4201 acaaatgatt ggtctaagat tggctatcct ttattactag ctttatttga agagacgatt
     4261 tatcgttttt tgtggtttaa tatattagct tttcaatggc atttaccaac aattattgtt
     4321 ttaattgtaa ctagtttttg ctatgcttta aatcacttat tgatggggaa atccattttt
     4381 tatgctaagt tggtaacagg catcatttat ggtagtattt acatgctgac tagtcaattg
     4441 tggttggttg tgataatgca tgtaggaggt aacttgctag ttgagtgtct tagccatctt
     4501 caaacaaaaa agaagaagga ggtgacatga tgctattggt tttgctgtcg tttttaggtc
     4561 taactactct ttggttgacc agttatcgaa gacgttgttt ggctagatgg catcttcaat
     4621 ggttaatggc cttatcctat caagattttt tagatgtctt actgagtctg tttcaatttg
     4681 tcgttattat acttgttttg ttcttttact cagctactat taatctagga gaggttctga
     4741 cctttttgac acaaactagc tggcattggc agattctttg ttatttagtg ttgtatctta
     4801 tggccatcat agagatgacg cttttagttt taattcttat ctttgatgtt ttgctccaga
     4861 aagatagtcg attgctcttt aaaaaaataa cttggctgcc ttttagacaa gataaaccag
     4921 tcttaagtat acttttgcta ggcatagtta ttctagtaga cagtctcttt tatttgggct
     4981 tgcttatatt attaggagaa cagtcgctaa caagtttagc aacgcttatt ttaggttatg
     5041 ggatagtcaa ggcttgtcgc tattcaggtt ggttaaatgt actattagct ttttgtcttt
     5101 tcactatagt tggactatgg gcagttacgg caactttatt atatggttgg ctagtaggtg
     5161 cgatgatatt aacggtgact tatttgatga ttagttgcaa agagtattag caatattaaa
     5221 aaggagtgat atatgagttt tgtacaatta acaaatgttg tcaagtccta caaaaacggc
     5281 aagaaagctg tcaatgatgt ttccttgtct attgaagcag gtaatattta tggtttgtta
     5341 ggaccaaatg gtgctggtaa gtctaccttg attaatctta tcttaggctt gatccctttg
     5401 agttccggta aaattactgt tttagggcaa tcccaaaaga ctattcgaaa aataagttcg
     5461 cagataggtt atgttcctca agacattgct gtttatccag acctaactgc ttatgaaaat
     5521 gtagaactat ttgggtcact ttatggctta aagggagctc agcttaaaaa acaagttcta
     5581 aaaagtttag aatttgtggg actacactcc caagctaagc agtttccaag tcaattctca
     5641 ggaggaatga agagacggtt aaatatcgct tgtgcgctag ttcattcacc taaattaatc
     5701 atttttgacg aaccgactgt agggattgat cctcaatcac gtaatcatat tttagagtcg
     5761 attcgtttgc taaacaaaga aggtgccaca gttatttata cgacccatta tatggaagaa
     5821 gtagaggctc tttgtgatta tatttttatt atggatcatg gtcaagttat tgaagaagga
     5881 cctaaatttg aactggaaaa acgttacgtt gcaaatctag caaaccagat tattgtaact
     5941 ctaacagatt cacgtcattt ggaactggca gataagcctg attggtcttt gatagaagat
     6001 ggagaaaaac tcatgttgaa gattgataat agtgatatga catcagttgt tcatcagctc
     6061 acacaggcca atattacttt tagcgagatt agacataacc atttgaattt agaagaaatt
     6121 ttcttacact taacaggtaa gaagttacga gattagaggt tactatggtt ttatttcatt
     6181 taatcaaaaa agaaagttta cagattttta gaaaccgaac agccttattg atgatggtga
     6241 tttttccaat tttgatgatc gttattttaa gttttgcctt taaatcaagt tttaatactg
     6301 cgacaacagt ccctaaattg accattcgtt accaattaga gggtgaaaaa acggattacc
     6361 agaaaaattt tcttgctttt ttaaaagttt taaaccaaaa acttcattta gaggctaaac
     6421 ctagtaattc tcttgaaaaa gatcgacaaa gggtcagtga aggagcctta acggctgttt
     6481 tagaagtgaa gaagaatcag accattaagg ttattactaa taatattaat cagcaaaatg
     6541 cagatttaat caatatgcta gtaaaaaatt atgtagataa tgctaaaact tatgactcga
     6601 tagcagctct ttatcctcaa caattaaatc atatcagaaa gcgaagtgtg gactatgtta
     6661 aggtcagttc aatacagaca agtaaaggaa tgacatcagc tgattattat gctatttcca
     6721 tgtttaccat gattactttt tatagtatga tgtctgcgat gaaccttgtt ttgtcagatc
     6781 gtcaacaacg gattacaaat cgtattcgct taacaggagt ttctccaagt cttttagtct
     6841 ttgggaaatt aataggtgct atgttagcaa caactgttca attgagtctt ttatacattt
     6901 ttacaaggtt tgttttacga gttaattggg gcactaatga gtggatgctt attggtataa
     6961 cagcctcctt agtctatctc tctgtagcta taggtatcgg gctaggcata agcattaaaa
     7021 atgaggcttt tttaacggtt gcatccaata ctattattcc tatattcgcc tttttaggag
     7081 gcagttacgt tccgttgaca acattacaca gctctattat taatcaattg tcga
//
LOCUS       DATURV010000069         7026 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DATURV010000069 DATURV010000000
VERSION     DATURV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.069"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(48..320)
                     /locus_tag="VCF75_001436"
     CDS             complement(48..320)
                     /locus_tag="VCF75_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEP2546989.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            complement(337..1698)
                     /gene="dnaB"
                     /locus_tag="VCF75_001437"
     CDS             complement(337..1698)
                     /gene="dnaB"
                     /locus_tag="VCF75_001437"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEP2546990.1"
                     /translation="MPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDDFY
                     KYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSANA
                     EYYAKIVAEKAMLRDIIARLTDSVNLAYDEILKPEEVIAGVERALIELNEHSNRSGFR
                     KISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGKTA
                     FVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNV
                     TIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKPEN
                     RQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADI
                     VAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIAQF
                     EER"
     gene            complement(1734..2186)
                     /gene="rplI"
                     /locus_tag="VCF75_001438"
     CDS             complement(1734..2186)
                     /gene="rplI"
                     /locus_tag="VCF75_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEP2546991.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLAITEA"
     gene            complement(2183..4159)
                     /locus_tag="VCF75_001439"
     CDS             complement(2183..4159)
                     /locus_tag="VCF75_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEP2546992.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            complement(4249..6147)
                     /gene="mnmG"
                     /locus_tag="VCF75_001440"
     CDS             complement(4249..6147)
                     /gene="mnmG"
                     /locus_tag="VCF75_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEP2546993.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDADYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            complement(6271..6588)
                     /locus_tag="VCF75_001441"
     CDS             complement(6271..6588)
                     /locus_tag="VCF75_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEP2546994.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRTKVNQLAFVVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
BASE COUNT         2152 a         1478 c         1080 g         2316 t
ORIGIN      
        1 taattatatc aatttattgt aaagaaaaag cctctatgag gcaaaaatca atctaaataa
       61 cgaattaatg ttttttctgt caaaatatct gaataggtgt atgattcgac ataagaatta
      121 tcaattgctc ctggagtatc agagctatca ctatactcaa tgataaagag tgaactgtaa
      181 acctcaataa gcctaccaat tttatttttt tcccgtttcc gtccattctc aagggttaat
      241 tctactaact gtccttcatg ggctcttatg tcctctttaa tttttttcat ttttgcaaca
      301 tctgtaaatg catcgctcat tttttgtctc tctttcttat ctttcttcaa actgggctat
      361 acttgagaat ttgttatatt ctttttggaa catcagtttg actgttccac gcgccccagc
      421 tctatttttc tcgaggataa cttcaattgt gttatcttca acagcttctt cagcatcatc
      481 accttcttta cggtaataat cgtcccggta taagaaggct acaatatcgg catcctgctc
      541 aatagatcct gattcacgaa tatctgataa aactggtcgt ttatcttgcc tttgctcaac
      601 gccacgagaa agctgactta gggcaataac tggtactttc aattctttag ctaggatttt
      661 aagctgtctt gaaatatctg aaacctcttg ctgacgattt tcgggtttag ttcctgtaat
      721 caactgtaag tagtcaatta cgataagacc taaaccacca tccacttctt gagacaattt
      781 ccgtgatctt gcgcggattt cagtaatttt aatcccgggc gtatcgtcaa tataaatcgg
      841 tgcttctgcc aaggctccct gagcaattgt tacattattc cagtcctgat ctgtgagttg
      901 ccctgttctc aaactgtgcg aatcaaccat tccttctgct gcaagcatac gatctactaa
      961 actttcagca cccatttcca aagaaaaaat agcaacagtc tttttttgct tagtccccac
     1021 attttgcgca atattaagaa caaaggcagt cttccccact gctggccgag cagctaaaat
     1081 aactaattga tctgggtgta aacctgttgt aatcttgtca aggtctctaa aaccagttgg
     1141 taaacctgta acatttgaag tctgcttaga acgtgcttct aaagcctcgt aattaacttt
     1201 taatacatct gaaattttgc gaaacccact acgattacta tgttcattga gttcaattaa
     1261 agctctctca actccagcga taacctcttc tggttttaaa atttcatcat aagctaggtt
     1321 gacagaatct gtcaacctag caataatatc acgcaacata gctttctcag ctacaatttt
     1381 agcataatat tctgcattag cactagttgg gacactatta actagttcaa caatatagga
     1441 taagccacca atactttgca gatcatcttg atcatctagg attgttctta tagtggttgc
     1501 atcaatggca tcattacgat cgctgagagt aatcattgcc cgaaagataa ttttatgagc
     1561 gtacttataa aaatcgtctg gactgataaa ttctctcact gcaatcagct tatcaggtga
     1621 gataaagatt gacccaagaa cagattgctc cgctagtaaa tcttggggtt gaactcgtaa
     1681 ttcagctact tcaggcaacc tcataacctc caaatcttgt gatgtttttt atattatgct
     1741 tctgtgatag ccaatttaat ctcagctgtt acttccttat gtaatttcac aggaacttca
     1801 attagaccaa tggcgcggat aggatgatct aaaacaatat gacgtttatc aacttttaca
     1861 ccaaattgct tttgcaattc ttctgaaatc tttttagctg tgatggaacc aaatgtacgt
     1921 ccatctggac caactttttc ttgaaattga acacgtgttt tgtcctcatc caaaacagcc
     1981 ttgacagctt gagcttctgc taatatttca gcttgagctt tttcttcagc tttttgtttt
     2041 ccttttaatt caccgatgct ttgactagtt gcttctttag ctaaattttt cttaataagg
     2101 aaattttgag catagcctgt tgggacctct ttgatttctc cttttttacc ttttcctttt
     2161 acatctgcta aaaaaataac tttcatgatt ctacttctcc tgtttctttc attgtcatat
     2221 taatagtttt caataataag tgctttgctt ggggaagact gatatccgtt aactgacagg
     2281 cagcaagatt aaagtggcct cctccaccta atttttccat cacacgttga acattgattt
     2341 tactacgact tctagctgaa atagcaatct tatgagaagc tgtttcaact aatacaaagc
     2401 tagcctcaac gtgagccatt gacagtatcg tatctgcagc tttactagca ataacattgc
     2461 tataaagatg atttttttct cctgcagcta caatgatact gtcaccaaga cgttctcctt
     2521 gtaaaataat ttcatttatt tgcttatatt cttcaaaatc tgttgcggat atattttgaa
     2581 tttcaacact atcacttcct ttacttctta agtaactagc gacatcaaag gtacgacttg
     2641 tcactcgcgt agaaaaattt ttagtatcaa gcataatacc tgccattaaa acactggctt
     2701 ggatcttatt taaacgtttt ttagcatttt gaaattgaat taactcagtg acgagctccg
     2761 cagcactact tgctccactt tcaataaaag ttaagatagc attatcagga aaatcatcat
     2821 ctcttctgtg atgatcaact acaataacat tttgaaactg ttcataaaat tctttagaaa
     2881 gtgttaaaga tatcttagaa tgatcaacca ttactaaaag tgatcttgga gtcactaaac
     2941 ccattgcttg agaaacactg attagacgag tctttccgtc agcctgcaat cgctcaatag
     3001 ccctttcaat atctggactc atctcatctg gattgtaaac ggcaaaacta ttttcaataa
     3061 tgttacctgc gaaaaattgc ataccaacag ctgaacctag tgcatccata tcaagtttcc
     3121 tatgtccaac aataaaaaca ttgtccacca tcttgattcg atctgaaata gctgtcatca
     3181 tagcacgggt acgtgttctt gaacgtttaa ctgtagaaac agaccctccc ccgaaataaa
     3241 ttggattcgt atgatccgca ttttcacgaa tgacaatctg atcaccacca cgaacaagcg
     3301 caatattaag attctcaaga gctacctgtc caatctggct atgattttct tcaccaaaag
     3361 aaattcctat gctgagtgtt aaagggcgct gagcatcttg agcttcttta cgaaactctt
     3421 ctaagacaga aaatttatta tccatcaaat catttaatgt tttaaagtct gtaaagaaat
     3481 agtagcgatc catattaaca cgacggtaaa aaatccgttt tgactccata aactcatcaa
     3541 tgaagttagc tacaaatgaa ttaatttttg aggtatctgc atctgataaa tcatcagtga
     3601 tatcatcata attatcaacc gaaataatgc caacaaccgg tcttaacata ctagcatcag
     3661 ctaattgcct gtttcccaca aagctatcaa aaaaataaaa aattcctgat gatacatcaa
     3721 tataagaagt gtatttatta ccagaaactt caaatgtttg agaaatatct tcacgacgtt
     3781 tttccgtaat aatttgctga atcaaactgt tttgtataaa accctcttca gttgtaaaaa
     3841 ttaattctgc ataaggatta taccattcta cagcgttggt ctcttgatca aattgaacta
     3901 ctccaacagg catattatca agtagcgttt ttaggttatc ttctgtttgt tcatttaaga
     3961 gctcaatatg agctaagtct gataattgat aagcttcttt ttgataccat aaaagagcaa
     4021 caacaaatag caaaacgaga aaaatagcta ataatattaa tatttttgat tgcataatac
     4081 tgacacaaag tgccagtagt ccaaataaaa tcaatcccat cataatcaaa tgaatggttt
     4141 caaaacgaaa ttttttcatc ttataacctc ttaagcacac attataccat aaaaaagccc
     4201 tgaaatacag agcttccact tgctttgaga ttacaatttc atttccattt aatattttcg
     4261 atgagctttt ccgtttccct ctaaataaat catcaagata ctgatgtccg caggattgac
     4321 tcctgaaata cgactagctt gtccaattgt ttcaggatta attttcttaa atttctgacg
     4381 cgcctcagtt gcaatagaat caatagcatc ccaatcaata tttgcaggaa tacgtttttc
     4441 ttccatgcgt ttcatcttag caacctgatc taaagctttg cgaatatagc cttcgtactt
     4501 gatttctgtt tctaaaagtt caatgatttt agcatctaaa tcttcagccg caggaccaac
     4561 aaaagagact gcagtcgcat aatcaatttc tgggcgacgc ataaattctt tagctgtcat
     4621 agcatccgtc aatggtttaa aaccaagttc ctgcacacga tcgttggttg cttttactgg
     4681 ttttaatttg atactgttca gacgttttaa ttcgttatca aattggtttt tcttaatttc
     4741 gaaggccttc caacgctcat catcaaccaa cccaatgtcg cgaccaatct ctgtcaaacg
     4801 catatcagcg ttgtcatgac gtaaaatcaa acgatactca gctcgcgatg tcaacaatcg
     4861 ataaggttcc aaggttcctt tggtaactaa atcgtcaatc ataacgccaa tataggcatc
     4921 gctccgtttc aaaataagtt ctggcttacc ttgaactttc aaagcagcat tgattccagc
     4981 aataagtcct tggccagcag cctcttcata cccagaagtt ccattagttt gcccagccgt
     5041 aaagagtcct gaaattagct tggtttcaag tgtggcacgt agctggtgag gtaaaacaat
     5101 atcatattca atagcataac cagtgcgcat catttcagcc ttttcaagtc ctttgatcga
     5161 atgaatcaaa tctttttgga catcttctgg aagactcgtt gacaaccctt gaacatacac
     5221 ttcttctgta tcacgcccct ctggctctaa gaaaagttga tgacgttctt tatcagcaaa
     5281 gcgaacaatc ttatcttcaa tagatgggca gtaacgaggg cctacacctt taacaatgcc
     5341 agaaaacatc ggtgctctgt aaaggttctg attaatgata tcatgactag tttgattcgt
     5401 ataagtcaac cagcagggaa tttgatcttt gagatagtca gcatcttttg acataaatga
     5461 aaaatgatta ggtttgtcat cacctggctg aatttccgtt tgatcgtaat taatagaaga
     5521 tgctttgaca cgaggaggcg ttcctgtttt gaatctgcca atttctaagc cgagtttttt
     5581 taaattatct gctaaagtta ctgatgccaa actattatta ggcccagaag aatatttgag
     5641 ttctcctaaa ataatttcgc cacgcagagc tgttcctgtt gtcactacaa ctgccttagc
     5701 cgcaaatttt tgtcctgtgg ctgttaaaac acctacaact cgtccatctt caactagaat
     5761 atcatcaatc atcgtttggc gaagagtcaa attagcctgt ttttcaaccg tatgtttcat
     5821 ttcacgcgca tacagggatt tatctgcttg agctcggaga gcgcgtacag ctggtccctt
     5881 acctgtatta agcattttca tctggatata ggttttatca atatttttac ccatttcgcc
     5941 gccaagagca tcaatctctc taaccactat ccctttggca gatcctccaa tagaaggatt
     6001 acacggcata aaagccaaca tatcaaggtt gattgttgcc aataaagttt tacaacccat
     6061 acgactagtg gccaaactgg cttcaacacc agcatgacct gcacctatga caatgacgtc
     6121 atagctttct gtaaattcat gtgtcatttt tttaaaatcc tttatttcag tctattcatt
     6181 taaaatcata cgctttagtt tagccttcaa tgcgaacaat atgtcctggc catttagcca
     6241 actcggtttg taaaaactca gaaacaagat tcatttgatc cagttgattg attggaatcc
     6301 attcacaaac ctgtcgcttg cattctctat catttctagt ttagggtctt ctaaggggct
     6361 taccaaataa tgaaattcaa tattatgcca gaaaacatca tcgatatcaa aatgattctc
     6421 aaccacaaaa gccagttgat taaccttagt tcgaatacca acttcctcta aggtttcacg
     6481 taaaacagtt tcatgcgttt tttcacctac caaacttgta ccaccaatgg tgtaatattg
     6541 gtcagctgca tctctagtta aaaaattctt accatttcta acaatcaagc actagctcgt
     6601 aaactaaagt tatctgacct ctagtgattc taaatcccag tcctattagc catctcaatt
     6661 ctagagctaa accataaaaa gggccaaaac cctagaagat tgcaggacaa aaaaacctac
     6721 aactttcatg agctttgacc atcattccga ttgttattat ctattttaaa aaaatgatta
     6781 ttgattgtca atattgattt catcacagac ctttacagta atgtcaatgt ttttacatct
     6841 ctgtcaatct attgtaatgt ttttaaaatg attacaaaag tatcactatg tctaatctag
     6901 cgaggcgaga aataaaaatg tcatcagtat tatcatacga ttttttgata tcttctaaag
     6961 tagtaaaata cagataccgt tgtttctaag tctacaagta agaacgccga taacaaaacc
     7021 ataaaa
//
LOCUS       DATURV010000070         6848 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DATURV010000070 DATURV010000000
VERSION     DATURV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.070"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(10..2328)
                     /gene="pcrA"
                     /locus_tag="VCF75_001442"
     CDS             complement(10..2328)
                     /gene="pcrA"
                     /locus_tag="VCF75_001442"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEP2546995.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSAEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
     regulatory      2643..2729
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2857..4179
                     /locus_tag="VCF75_001443"
     CDS             2857..4179
                     /locus_tag="VCF75_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEP2546996.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNESVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFVLM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     gene            4299..5534
                     /locus_tag="VCF75_001444"
     CDS             4299..5534
                     /locus_tag="VCF75_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEP2546997.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLSLSVAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMIPEEEIFDNVAKNLYRYEKLILSKVPDYDHYIAKSFQLIDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQHTSIWRRHESWSLLFHQ
                     ITPIAKRQLHPTHYRIVKM"
     gene            complement(5904..6677)
                     /gene="cfa"
                     /locus_tag="VCF75_001445"
     CDS             complement(5904..6677)
                     /gene="cfa"
                     /locus_tag="VCF75_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEP2546998.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTVLMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATINKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKV
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKPFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
BASE COUNT         1924 a         1349 c         1278 g         2297 t
ORIGIN      
        1 atttttttct tattttttct caataggagc aacacttgct agtaatttct ttaaaccaac
       61 ttctggaaat ttgattttca attccatcgt ttttccggag cctgaaacct caagcacagt
      121 cccgtctccc cattttttat ggtgagcgat atcgccaatc tgccaagtga cctcttctgc
      181 agagctatta gaaccaaacg gtaaaactga gctagttgaa gctgatggta tcgtatgtgc
      241 ctgcattggc tgtgctgatt gtcttacttg agcctgggcc tttcgagctt gtaaggcttc
      301 tgataagctc atcccttgtc caaactgtgt gtgtgtttca gtagaaaaac gcacaccaaa
      361 tgaagattgt gctggtcttg ccaatccttt ataagagagt agttcctctg aaatttcttt
      421 caagaagcgt gttggacggt tataactact tttaccaaag agggttctgg tgttggcatt
      481 ggtcataaag agaacttctt cagctcttgt gattccgaca taagctaaac gtctttcttc
      541 ttccaattca tctggatctt cagaggcacg tgataaggga aagacacctt cttccatacc
      601 aattaagaaa acaacaggga actccaagcc tttggcagca tgtagggtca ttaaggttac
      661 ttcagcagct tctgcttggg aatcatctgt atcagcaatc agtgctaaat cgtttaaaaa
      721 acgtcccaag cgatcaacac cggtttcgtc ttcttcttga gaggcacttg actcgtcaaa
      781 atttttggta acagataaga attcttcgat attttcgata cgggcttggc tttccaatgt
      841 attttgcagg cgtaagctat ctaaatagcc tgttttttct aacaaagctt ctgctaagtc
      901 ggtgatactc atctgatcta ggtcttgacg tagctgaccg agaatattgg ccaagtccat
      961 gatagcttgt gctgctttac ctttcaaggg ggacatcaat agatttgaag ctgcttctaa
     1021 taaagactgg tcactctcat aggcaaactg acgcagttta tcaagcgttc ctggaccaac
     1081 acctcgttta ggctcattca caatccgctc aaaagaaata ttatcagcag ggttagcaac
     1141 aatggttaaa taggcaatca aatcccgaat ttctttacgg ctgtaaaact tggtgcctcc
     1201 aaccatggtg taaggaatat tggattttaa gagtgcttct tcaatggtcc gagattgggc
     1261 attggtacgg tataaaacag cgaaatcttt gaagtttttt ccaagctctt gactcatatt
     1321 gctaatggtt gaggccacaa atactgcctc atctcgctca tcatttgccc tgtaataaac
     1381 cagttgttcc ccatccgcat tttgtgtcca aagtttctta ggacggcgat tacggttatt
     1441 gttaatcacg tcgttagctg cttgcagaat tttcttggta gagcgatagt tttcttctaa
     1501 aagcactacc ttagcatctg ggtaatcttt ttcaaaatca agaatatttt gcatgtcagc
     1561 tccccgccaa ccatagatag actgatcagc gtctccaaca acgcaaatat ttttaaagcg
     1621 agaagctagc aatttaatta gctgatattg agcatggttg gtatcttggt actcatccac
     1681 atgaatgtat tggtaacgct gctggtaata agctaacacg tcaggattag cgtcaaacag
     1741 gcgtagcgtc atcatgatta aatcatcaaa gtcaagtgct tcgctacgtc gtaattcttc
     1801 ctgataagct ttgtaacacc tcgctacgat ttgactatac atatcagcag cctgtgcctc
     1861 gtatcccttt tcatcaagca aatcattttt agcgtttgaa atagttccta aaatagaacg
     1921 ttcattccac tttttagggt ctatatttaa ttgttttaaa atgcgcttca tcaacgttcg
     1981 ttgctcacct ggatccacga ttgtgaagtt tcggttgtaa ccaatgtgat cagcctcacg
     2041 acgcagaatc cgtacacaca tggagtgaaa ggtagcaatt aaggtatctt tagtagctgg
     2101 atttaaggcc aaggctcttt ctttcatttc acgcgcagcc ttattagtaa aggtaatagc
     2161 taaaatattc caaggattga caaatttttc atcaattaaa taggcaatac gatgggtcag
     2221 tacccttgtt ttaccagaac cagcccccgc cataatcaat aaggggcctt cagttgtttg
     2281 aacagcttgt gcctgccgat cattcattcc atttaataaa ggattcatgc tttctcctta
     2341 atctcgttca gtgacatctc ttttatcaac cttaaaatta tatcatgttt ccaagaaaaa
     2401 tagcaaagaa gaccccaaaa gtaaagagat tccttttttc tagtttaggt tcagatattt
     2461 aaaaataata gcgatttctt aaagcattcc tcattttttt gctattttgg ttgacttcat
     2521 tcgttttttg aggtatgata gtcctaattt aataaaccga atgatgtcat gcaggagaag
     2581 aatttttttc gccgaaggag ttatactctc aggtgttcag tttttgaacg ggactgtttg
     2641 atggacggac ttctggagag accttattag gcgccgaagg ggcaaggcat actgctcaat
     2701 ctctcaggca aaaggacaga aggtaaaata caaacaccat taagaacagt cttagtcttt
     2761 tttgtgtttg ctgttttatc attgcttcag aagttgtctc aaagaaagag atagcttttt
     2821 tcttttggcg tcttcgatga cttttaggag agaaagatga tagcactcgt taaattaatt
     2881 gataaccttg tttggggacc gcccctctta attttattgg ttgggacggg gatttacctt
     2941 accagtcgtt taggattaat tcaaatctta aaactaccaa gagcctttaa actcattttt
     3001 tcagatgacg aaggacatgg agatatttca tcctttgctg ctcttgcaac tgcccttgcc
     3061 gctactgtcg gaactggtaa cattgttggg gttgccactg ctatcaagtc tggtggtcct
     3121 ggagcgctct tttggatgtg ggttgccgct ttttttggaa tggccactaa gtacgctgaa
     3181 ggggtactag cgattaaata ccgtacaaag gatgccaacg gtcatatttc tggtggtcct
     3241 atgtactata ttgttaatgg catggggaca aaatggaaac ccttagccat tctttttgcg
     3301 ggttcaggga tcctagttgc cctatttgga ataggaacct ttgctcaagt gaactcgatc
     3361 acctcgtctt taggtcatag ctttggctta tccccacaaa tggtcagtat tgttttagct
     3421 atctttgtgg ctgccattat ttttggtggc attcattcca tttccaaagt ggctgaaaaa
     3481 gtcgttcctt ttatggctat tttttatatt ctatctagtt tagcagttat ttttagtcac
     3541 taccagcagc tacttcctgt tattcgttta gtctttcaat cagccttcac tccgacagct
     3601 gctattggag gctttgctgg aagtctcatg aaggatgcca ttcaaaaagg aattgcacgc
     3661 ggtgtcttct ccaatgagtc tggactagga tctgcaccta ttgctgcagc agcagctaaa
     3721 accaatgaat ccgtggagca gggcctgatc tccatgacag gaacttttat tgatacgatt
     3781 attatctgta ccctaaccgg cctatctatt ttagtaacag gccaatggac tgggcagctc
     3841 gaaggggctc ctctgaccca atctgccttt gccactgtgt ttggaaattt agggactttt
     3901 ggcctaacct tttccctagt tctcttcgcc tttacaacga ttttaggatg gagttattat
     3961 ggagaacgct gctttgaatt cctctttggc attacacatc tcacctactt ccgtattgtc
     4021 tttgtcttaa tggtaggcct aggaggcttc cttaagttag aactgatttg ggttttggct
     4081 gatattgtca atggtttaat ggcacttcct aacctgattg ccctcctagc tctctcgcca
     4141 gttgttattt tagaaaccaa gcattacttt attaagtaaa catgacgtta gtactaccca
     4201 gttataccta gtcttcttct gccaagttca agacgctagg cttttttctt aagccccttt
     4261 ttatggtata atataaagtt gaaatcaaag gagttattat gacacaggat ccgattgcaa
     4321 atttaaaact agccagaaag ggcccaatcg ttagtattat tgtctacttg tcgcttagtg
     4381 ttgccaaatt attagctggt tatttgttaa atgcaagttc ccttattgct gatggattta
     4441 acaatttatc ggatattgtg ggaaatgtag ccctgcttat tggtcttcac ttagctagcc
     4501 aaccagccga tgccaatcat aaatttggtc attggaaaat tgaagactta tccagccttg
     4561 tcacttcttt tattatgttt cttgtaggtt tccaagtact gattcacaca attaaaagta
     4621 tctttagtgg tcagcaagtt gatattgacc ctcttggggc gattgtcggt atcgtttcag
     4681 cttttgttat gttaggggtt tatgtcttta acaaacgtct ttccaaacgt gtaaaatcca
     4741 gtgccttagt cgctgcttct aaggataatc tagctgatgc tgttacttct atcggaacat
     4801 caattgctat tatagcagct tctttgcatt taccagttat cgatcatata gctgctatga
     4861 tcattacgtt ctttattctt aaaacagctt ttgatatctt tatggaaagt tcgtttagtt
     4921 tatctgatgg atttgatagc cgtcatttga aaaaatacga aaaagccatt ttagaaatcc
     4981 ctaaaattgt cgccgttaag tctcaacgag ctaggaccta tggtagcaat gtctatcttg
     5041 atattgtact tgaaatgaat cctgatcttt cagtctatga aagtcactct attacagaga
     5101 aagtggagca gttattgagt gaccaatttt ctatttatga cattgacatt cacgttgagc
     5161 ctgccatgat tcccgaagaa gagatttttg ataatgtcgc caaaaatctc taccgctacg
     5221 aaaaattaat tttgagtaag gttcctgact atgaccacta cattgctaag tctttccaac
     5281 tgattgatgc gaatggccaa acagttaact atgaacaatt tttgaaccaa gaaatttatt
     5341 atccaagtaa cttcaaccat tttcagattg aatccattag tcaaaaaacg atgttggtaa
     5401 cttaccaatt aaatggtaat caacatacca gtatttggag gcgtcatgaa tcttggtcct
     5461 tactcttcca ccaaattacc cctatcgcta agagacaatt acatcccaca cactaccgta
     5521 ttgtaaaaat gtaaaaacat cacttctctt ataacataac tatacttcat gatattccta
     5581 aagcgattcc tagtcccaaa atgataagtg aaatagttaa aaaaatccct acaatttgtt
     5641 tttaaacaga tcatagggat ttttttaaga atacacgttt tcatattcaa tcttattagt
     5701 ttaggaggtt aaacaagatt caatagtgac ggttgaacaa taccttaaat tggcacatca
     5761 tgttaaaggc aaaaagggct acaggtgact cattgtttat gagaacatca tctcaaactc
     5821 tacaatggta agatatttaa aatctttacc tattctatat cctaatataa tcatctacct
     5881 atacaacaca ttacttatac actctatgac ttaagagctg tttccaaagc attttgcaca
     5941 gcaataactt catcgtcaac ttgttgaaca gtggttgtcg gatttagttg gacaccaacc
     6001 gcatgtgtga tagctttgtt aagtctattg taaacgtcaa atggtttgat ccctaaaacc
     6061 tttttatctc tttctaaacg agtgttccaa atagctttat ttagtttagc tttagtatag
     6121 atagtagcac ggtctgttgg ctgtaaatca ggataattga taactttttc ctcaagtgct
     6181 ttaatttctt gtacttgaac tttgatagcg tcaacagatg caaatggatc tataatacga
     6241 atgactaatt tagtaatagc aaaccccata tcagtatgcg ctttttctac tttatgagtt
     6301 aattgggttg tggaaaatac gattgctttg atagcatctg ataatgcttc tactcttgcc
     6361 ccaattgaac taaaatcata gatcgtttca gtattgaagc gtatagaact cttcatgtga
     6421 tcaacagcat cgatagcttt gttaatagta gcctcaatat cagtaccttt agcttcatgt
     6481 ttaatagctt ctaattgttg atgcccctca tcaagctttt gcactagagc atctctttga
     6541 agattcgctt ggttgttatt cattaaaaca gtatcgggat ttgaagcatc atcagctaaa
     6601 actgagtgcg tcatagttga aaataaagct aatccagcaa caactgttga aagacagaga
     6661 aggtgtttgg gtttcatatt attcctcctg aatataataa acctatttaa atagttaagt
     6721 gaagttaaat taatttataa gcgcttataa cgttatcata ctacttgttt ttactaaggt
     6781 caatatttct ttaatttcca gaaaataaga ggatttcacg aaaataatca ttttttaatg
     6841 tcattaaa
//
LOCUS       DATURV010000071         5952 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DATURV010000071 DATURV010000000
VERSION     DATURV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.071"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..767)
                     /locus_tag="VCF75_001446"
     CDS             complement(<1..767)
                     /locus_tag="VCF75_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP2546999.1"
                     /translation="MQLRPYQEEARSAVQHEWEDGKKRTLLVLPTGCGKTIVFSKIIE
                     DRVRLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAENTSLGSWFRVVVGSVQTM
                     QREKRLSKFPTNHFDTIIIDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLTLDLSTVSQQAGDFKASEIGTALDPYL
                     EQIADEMVKQCANRKTVVFLPLVKTSQKFRDILNQKG"
     gene            complement(767..1402)
                     /locus_tag="VCF75_001447"
     CDS             complement(767..1402)
                     /locus_tag="VCF75_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547000.1"
                     /translation="MTEYNNNFDRELGWDDEITQDSVGFVQLVPGDYQFTVTNMERAR
                     HTPNPKSPGKLPACNKAVVTIQIETSEGIAQLTHNLFLHTTTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVIGAKGVARINKRKGTGDYADREYDNIKSMIYADEVDWTKVLNKDVAQ
                     PQQMTHQQSAQPTYQGQQAQGTNFAQQPATQSYQQGQMQTPQQPQGGWGGF"
     gene            complement(1424..2158)
                     /locus_tag="VCF75_001448"
     CDS             complement(1424..2158)
                     /locus_tag="VCF75_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547001.1"
                     /translation="MTENIKEALEYAVNTAHEKPKTILGSDGKEYYDRNKYSLVELKT
                     KYYPKTLNLNTLDSLIDYLKSDMNNINSKRLMVIVEGPREVIVCEEDDDDLNRNVLVT
                     VEAIIPNVQFGRYDSPSDFNISLQSLFVNADDRNTVIEFASALKIENGSEIVDDGIGQ
                     TATIKQGVASLAKAKAPNPVTLRPYRTFSEVEQPSSEFIFRINQLANMALFEADGGKW
                     RLDAINNIANYLKEELADQTNITILA"
     gene            complement(2160..2582)
                     /locus_tag="VCF75_001449"
     CDS             complement(2160..2582)
                     /locus_tag="VCF75_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547002.1"
                     /translation="MNKTIKLDLSAIGEGGLQEKVDKELEKVFDNILDPNTETKVARK
                     VTITLTMKPDDARQTVSTAMEVKSTLAPQTGVATTVLVGQKDGKVYANELKSAMPGQT
                     YFDDEAVLRTDIGEPIDHLEKGINEDVIDFNKQKKVGN"
     gene            complement(2622..3713)
                     /locus_tag="VCF75_001450"
     CDS             complement(2622..3713)
                     /locus_tag="VCF75_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP2547003.1"
                     /translation="MQITRGKRARAQRVIIYGPEGIGKSSFAANFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAWIKANPTCCKTLVIDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNMLQELIELGINIVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTSESKKKKATGGQRVLYTQHHPAWDAK
                     NRHGLPDEMPLDYAVIAHLFAQVPTQPVPQTPPVQETPASQTAHESVHEQAEKAPEQP
                     PMQPTSAPVAYPPSMPKALTDLMSAEQVTPDELVAVANIRGHFPPMTPIENFPSDYWN
                     MIVANWLATLEVIKTQVRTVEPPFTVEGA"
     gene            complement(3728..5047)
                     /locus_tag="VCF75_001451"
     CDS             complement(3728..5047)
                     /locus_tag="VCF75_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2547004.1"
                     /translation="MSITINKLEIENVKRIKAVKIEPSATGLTIIGGNNNQGKTSVLD
                     AIAWALGGNKYKPSQAMREGSQVPPTLKITMSNGLIVERKGKNASLKVIDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDSTPKEKADVLLEIIGVGDQLAELELKEKEIYNQRHAIGV
                     IADQKEKFAKEMTYYPDAPKQLVSISELIQQHQAILAKNGENAQKRQNVERIRYDYNQ
                     SILEVDRLRKLLADAEAKTNKLSEDLKIANTDAMDLHDESTAEIEANIADIDEVNRKV
                     RANFDKDKAEEDAKQQREQYNILTNDIESIRQQKRDLLINADLPLEGLSVDDGKLLYL
                     GQEWDNMSGSQQLIVATAIVRKLKPDCGFVLIDKLEQMDNITLEQFGKRLEQEGLQAI
                     ATRVSIGEECAIIIEDGYSVENKAHNFKTAEGGNFAETVAPTWKGEF"
     gene            complement(5047..5274)
                     /locus_tag="VCF75_001452"
     CDS             complement(5047..5274)
                     /locus_tag="VCF75_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547005.1"
                     /translation="MKQQLINDNEFLRDENRRLNNELAEHYFVVTAKANLLDVIIASD
                     FILKSTLDKCIAELDEIDQIALLKAWNKGEL"
     gene            complement(5398..5535)
                     /locus_tag="VCF75_001453"
     CDS             complement(5398..5535)
                     /locus_tag="VCF75_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547006.1"
                     /translation="MKNKKEQWTPKVHCFRKDGSQCEPENITVPISFAGYYQILLELG
                     V"
     gene            complement(5604..5834)
                     /locus_tag="VCF75_001454"
     CDS             complement(5604..5834)
                     /locus_tag="VCF75_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2547007.1"
                     /translation="MTINLKRLKAERIASGMTQCEVAQSMGWKTRTPYAKRENGIVSI
                     GADELAKITLIFGLPIEKIAIFFDKDVPVMER"
BASE COUNT         1523 a         1248 c         1060 g         2121 t
ORIGIN      
        1 aatccttttt ggtttagaat atcacgaaat ttttgggaag ttttaacaag cggaagaaat
       61 acaactgttt tacggtttgc gcattgcttc accatttcat cggcaatttg ttctaggtac
      121 gggtcaagcg ccgtcccaat ctcacttgct ttaaaatctc cagcttgctg actaactgta
      181 gataagtcca aagtcaacgg gatagtaact gcggtaattt tagataagta accagacttg
      241 atggcatcta ctaaggaata ttcataagct aaactatcga aatacttacc tagattttgt
      301 ttgtcaccgc gatctggtgt cgctgtcact cccaaaacat tgctactatc aaagtgttgc
      361 aaaactcttt ggtaaccatc agagatggca tgatgggctt catcaataat aattgtgtca
      421 aaatgatttg taggaaactt actcaaacgc ttctctctct gcatcgtttg aactgatcca
      481 actacaaccc tgaaccatga ccctaaagaa gtattctctg ctttttctaa tgctgtccct
      541 aatccagttg ctgtcatcaa tttatcacta gcctgttcta aaagttcgga cctatgagcc
      601 agaaccaaaa ctctctctcc gagccttaca cggtcttcga taattttcga aaagacaata
      661 gttttaccgc aaccagttgg aaggacaaga agagttctct tcttgccgtc ttcccactcg
      721 tgttgcactg ctgaccttgc ctcttcttgg taaggtctaa gctgcattag aatcctcccc
      781 atccgccttg aggttgttga ggtgtttgca tttgaccttg ttgatatgac tgcgttgctg
      841 gttgttgtgc aaaattagtg ccttgtgcct gttgtccctg atatgtaggt tgagcagatt
      901 gttgatgtgt catttgctgt ggttgtgcga catctttatt taatactttt gtccaatcaa
      961 cttcatcagc gtaaatcata gatttgatgt tatcgtattc gcgatcagca taatcgccgg
     1021 tgcctttacg tttattaata cgagcaaccc ccttagctcc gatgacggta ttccagttca
     1081 ttttaagtgg ttccccatgt ttcttttgac caatagctcc aaagaaagct gatagcatac
     1141 cttctgttgt agtatgtagg aataagttat gagttaattg cgcgattcct tctgatgttt
     1201 ctatttgaat cgtaacgacc gccttgttac acgctggtaa ttttcctgga ctcttcggat
     1261 taggtgtgtg acgagcacgc tccatattgg ttactgtgaa ttgataatca ccaggcacta
     1321 attgaacaaa acctacactg tcttgtgtaa tttcatcatc ccatcccaat tcacggtcaa
     1381 agttgttatt gtattcagtc atttttattt ctcctatttt ctattaagct aaaattgtaa
     1441 tgttagtttg atcagcaagt tcttctttta agtagttagc aatattattg atagcatcta
     1501 agcgccactt tccaccatct gcttcaaaca aagccatatt agcaagttga ttaatgcgga
     1561 aaatgaactc acttgaaggt tgttctactt cagaaaatgt gcggtatgga cgtaatgtaa
     1621 ctgggtttgg cgccttagct tttgcaaggc ttgccacacc ttgtttaatt gtggcagttt
     1681 gaccaatgcc atcatcaaca atttctgagc cattttcgat ttttaatgca ctagcgaatt
     1741 caataactgt gttacgatcg tcagcgttaa cgaataatga ttgcagacta atattaaaat
     1801 cagaaggtga atcatagcgg ccaaattgaa catttggaat aattgcctcc acagttacta
     1861 acacatttcg attaaggtcg tcatcatctt cttcgcaaac aataacttca cgagggcctt
     1921 ctacaatgac cataagacgc ttggaattaa tattgttcat atctgattta agataatcaa
     1981 ttaaactgtc aagcgtgttt aaattcaatg tttttggata atacttagtt ttaagttcaa
     2041 ccaaagaata tttgttgcga tcataatact ctttaccatc actacccaag atagtcttag
     2101 gtttttcgtg agctgtattt actgcatatt ccaaagcttc tttaatgttt tctgtcatgt
     2161 tagtttccta ctttcttttg cttattaaaa tcaataacat cttcattaat acctttttca
     2221 aggtgatcaa ttggttcacc gatgtcagtt cttagcactg cctcatcgtc aaaataagtt
     2281 tgaccaggca tcgcgctttt gagctcgtta gcataaacct taccatcttt ttgaccaaca
     2341 aggaccgttg tagccacgcc tgtctgtggt gctagcgttg acttaacttc catagctgtt
     2401 gaaacagttt gacgggcatc gtctggtttc atagttaacg taatagttac tttacgggct
     2461 acttttgttt ctgtattagg atctaagata ttgtcaaaga ctttttccaa ttccttgtct
     2521 actttttctt gtaagccacc ttctccgatg gcagacaagt ctaatttgat tgttttattc
     2581 attgtttgtt atacctcgct ataatttcta attcccaaaa cctatgcgcc ttccacagta
     2641 aagggtggct cgacagtacg cacttgcgtt ttaatcactt ctaacgtagc taaccaattg
     2701 gccacaatca tattccaata atctgacggg aagttttcaa ttggtgtcat tggcgggaag
     2761 tgtcctcgga tgtttgctac tgccacaagc tcatcaggag tgacctgctc tgccgacatc
     2821 aagtcagtca atgcttttgg catacttggt gggtaggcta ccggcgcaga tgtcggttgc
     2881 ataggtggtt gttctggtgc tttttcagct tgctcatgta cggattcatg tgcggtttgt
     2941 gatgcaggag tttcctgtac tggtggcgtt tgtggcactg gttgagttgg tacttgtgcg
     3001 aatagatgtg cgattactgc atagtcaaga ggcatttcgt ctggtaaccc atgacggttt
     3061 tttgcatccc acgctgggtg atgttgcgtg tatagcacac gttgaccacc agttgccttt
     3121 ttcttttttg attcgctagt cataactact gttttgtagt tggcaaacaa gaccatatca
     3181 gcccattctt tgaccaaggg ggcagtctgt gagcttgtct ttttaccaag tttcaactcc
     3241 caacggtcat aagctcccat ctcgtcaggt tgttcaaatt ttctcatttg tgcatgggca
     3301 gttaatacaa tattgatacc gagttcaatc agttcttgga gcatattcaa gaatcgacct
     3361 acttcctctt ttgtgtaggt ataaccattg ccccaaccaa agtcctcgat acctttcttg
     3421 ccatgttgag cacagacatc atctactatt aagctttctg cccaatcgat tgtgtcaatg
     3481 accagtgtct tgcaacatgt tggattcgct ttaatccatg caatttgatt ttttagcata
     3541 gtgtagcttg ttggtttatc catacgggcc acatccatat tgtctgttga tccctccgta
     3601 tcaatgaaca atggttcagg aaaattagca gcaaaactag atttaccgat tccttctggt
     3661 ccataaatga tgacacgttg agcacgtgct cgtttacctc ttgtaatttg catatttacc
     3721 tctctttcta aaactcacct ttccaagtcg gtgctactgt ttcagcaaaa ttaccaccct
     3781 ctgctgtttt aaagttatga gctttgtttt ccactgagta tccatcttct ataatgatgg
     3841 cacactcttc accaattgat acacgggttg caatagcttg caaaccctcc tgctctagcc
     3901 gtttaccaaa ttgttcaagt gtgatattgt ccatctgttc gagtttatca atcaaaacaa
     3961 agccacaatc tggttttagt ttacgtacaa tagcagttgc tactattagt tgttggctgc
     4021 ctgacatgtt atcccactct tgaccaaggt aaagtaactt tccatcatct actgataagc
     4081 cttcaagcgg caaatctgca ttaatcaata agtctctttt ttgttgacga attgactcaa
     4141 tatcattagt caatatgtta tattgttcac gctgctgttt agcatcttct tcagctttat
     4201 ctttatcaaa gttagcgcgc actttgcggt tgacttcatc aatatcggca atattcgcct
     4261 cgatttcagc tgttgattca tcatgtaaat ccatagcgtc agtatttgct atttttaaat
     4321 cctcgcttaa cttattcgtc ttagcttctg catctgctaa taacttgcgt aaacggtcaa
     4381 cttccaagat agattgatta taatcatagc gaatacgttc tacattttga cgcttttgag
     4441 cattctctcc gtttttggct aagatagctt ggtgttgttg gataagctca gaaatactta
     4501 ccagttgttt aggtgcgtca ggataatatg tcatctcctt agcaaacttt tctttttggt
     4561 ctgctataac accgatagcg tgacgttgat tgtatatttc tttttctttt agctctaact
     4621 ctgctagctg atcaccgaca ccaataattt caagcaagac atctgctttc tcttttggtg
     4681 tactatccat aaatttcggc aagttaatag ccaattcttc tacaaagcta tctagcaact
     4741 gctgaccgcc tttttggccg tttggatcaa ttactttaag actggcattt ttgcctttcc
     4801 gttcaacaat aagtccattt gacatggtaa ttttaagcgt aggtggcact tgcgatcctt
     4861 cgcgcatagc ttgactaggc ttgtatttat taccgcctag tgcccaagca atagcatcta
     4921 atacgcttgt tttgccttgg ttattattac caccgataat ggtcaagcct gttgcagatg
     4981 gttcaatctt taccgcttta atacgcttaa cgttttcgat ttcaagtttg tttattgtta
     5041 ttgacattat aattctccct tgttccatgc ttttaacagg gctatttgat caatttcatc
     5101 caattcagca atgcatttat ctagcgtact tttcaagata aaatcactag caataatgac
     5161 atctaataaa tttgctttag ctgtaactac aaagtaatgt tccgccaatt cattatttaa
     5221 cctgcgattt tcgtctcgca aaaattcgtt gtcgttaatt agttgttgtt tcatactttt
     5281 tttctcatat aagcgtggaa tgcatcatac tgcttttcag atgacaatcg cttagtatcg
     5341 tgtttaatcg gttcctgttt ttttggtttt gtaaaaataa aatctaatag tttcatgtta
     5401 tactcctagt tctaataaaa tttgatagta acctgcaaaa gaaattggca ctgttatatt
     5461 ttctggctca cattgtgaac catcttttcg aaaacagtga acttttgggg tccattgctc
     5521 ttttttgttt ttcatggtaa aattgcctca taagaatctg tctgtcccgt aaactgctat
     5581 tttacagggc ttttttattt gcatcatcgt tccattacgg gaacgtcttt atcaaaaaaa
     5641 attgctattt tttcgattgg taatccaaaa atcaaagtaa tttttgcaag ctcatcagct
     5701 ccaattgaaa caataccgtt ctctcttttt gcatatggtg ttcgtgtttt ccaccccata
     5761 gattgtgcaa cttcacattg tgtcataccg ctagcaattc gctcggcttt tagtcgcttc
     5821 aaattgattg tcatatacat actccttttt ttatttttgt tcccgttttg gaacaccttt
     5881 attataaaac ctacgttcca aattgtcaac gattttttct aaaaaaaata cgtttatttg
     5941 ttttttagcg tt
//
LOCUS       DATURV010000072         5556 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DATURV010000072 DATURV010000000
VERSION     DATURV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.072"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            26..181
                     /locus_tag="VCF75_001455"
     CDS             26..181
                     /locus_tag="VCF75_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lantipeptide"
                     /protein_id="HEP2547008.1"
                     /translation="MSFMKNSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
     gene            263..2566
                     /locus_tag="VCF75_001456"
                     /pseudo
     CDS             263..2566
                     /locus_tag="VCF75_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
     gene            2563..3300
                     /locus_tag="VCF75_001457"
     CDS             2563..3300
                     /locus_tag="VCF75_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2547009.1"
                     /translation="MNQLLLKNVCKRYPNQPNYALDHINLTVRKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISAFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNVDSSKINDLINNYAKRFNLYEQLEKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEETMMILSEINKTEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHLEIDKEDCGETFYDVISQALSDRGE"
     gene            3302..5209
                     /locus_tag="VCF75_001458"
     CDS             3302..5209
                     /locus_tag="VCF75_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2547010.1"
                     /translation="MIWSITKSNIKKNFSLYRIYFLATIGLLSIFIAFLNFISDKIIT
                     EKIGDSGQALVIANGSLVFLTVFLVIFLIYFNNFFVKKRSQELGVLAILGFSKKELTQ
                     LLTLENLVILVLSYLVSLLLGPTLYFLAVLVITHLLELTLEVQWFITANEIIESLGIL
                     VVVFLINVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAIIAITALLLSYILCLAT
                     VFSSTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLLKGNKKRLYRPLSNI
                     IYPKFNYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEYNVES
                     ENGQVNVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFDIINYSDYKELMKA
                     QGRINSIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTHNVFSFSTSVTTL
                     VVSDKLYAKLSSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDVISSYSKEYTVKTA
                     NIATYIFITFLSILFIICTGSILYFTSLIEIMENKEEYGYISKLGYSKKMIHRILRYE
                     TGILFLIPVFIGIVNGGMLLIYYKYLFMDTLVAGNIIMLSLLLCLLFFLIIYGTFYVL
                     TLRSVTSIIKN"
     gene            5249..>5556
                     /locus_tag="VCF75_001459"
     CDS             5249..>5556
                     /locus_tag="VCF75_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547011.1"
                     /translation="MIRIKNITKSKFFGTAIILLQQLIALLILVYNRENLSLLFSEKV
                     ALVMTLIDTAFIWLATILRQKQGDIFKRIIFIISLTIWQYLVSVLTRGTPLFLSSGLQ
                     "
BASE COUNT         1938 a          736 c         1006 g         1876 t
ORIGIN      
        1 tcactacata ctgatagaaa ggagaatgag ttttatgaaa aattcaaagg atattttgac
       61 taatgctatc gaagaagttt ctgaaaaaga acttatggaa gtagctggtg gtaaaaaagg
      121 ttcaggttgg tttgcaacta ttactgatga ctgtccgaac tcagtattcg tttgttgtta
      181 attaaaacaa tctggataag cagactaaaa tttcctgttt agacttagtc taggcaggat
      241 ttttaagtag tggtaggaaa aaatgataaa aagaaatgaa ttaaagttag aatatcagca
      301 acatcagttt tacgagtact ttaactcaat ttttgattac gacatattag atacttctat
      361 tagtgaaaat atttatttgt taagggaaaa gacacataag ttattttata gtgagtttga
      421 aaatcagtta tttgaaacta taatgtttct ttcaatgaaa acactagttc tagatataaa
      481 tcatttttcc aaggaaattg aaaacaagtc aaaggcatat gaacaatata tacagcagat
      541 aagggaagaa aatgggataa gtaatttttt tgacagatat ccatatttgc taaaacaaat
      601 caatagagaa gttggattgg tagtggaatc atactctttg ctatttgata ggtttttaaa
      661 agatttgtct gaaataaggt catgctttaa tattactgag tccttaagta atgttgaatt
      721 ttcacttgga gacagtcatt cccaaaaaca aacagtcgtt aagattgaat ttaaagggaa
      781 atccgtttac taaaagccta aaagctatga ttcatacaac atcttgttgg aacttattag
      841 tctattgaaa tctaataata taccttcgtt cagtcttcct gaaagcttaa ttaaagcgga
      901 ttattgttgg caacttggtg tagcttatac aagttccaat aaagatgaag tagcgaaaat
      961 ttactttaag tatggtgttt tagcagcatt ttctgaaata ttttctatta cggacttaca
     1021 tatggaaaat gtgattgttt caggcggaga tttatattta attgacgttg aaaccttttt
     1081 tcaaagaaag ttgaatgtac aaaatcagaa ttttgaaggg attactgtag acacctatca
     1141 gagaatttat aagacatcac tttcaaatgg tttatttcct gtacaatttg agaaaaacag
     1201 cgctcctaac gtttctggga taagtggaaa aggtgggaaa agaaaaaagg gaaagtatga
     1261 actgataaat aaaaatcgtg gagatatgaa gttagtgaaa actgactatt ttcaggaaga
     1321 tggttataat attccgacat taaatgggaa agtggtagag cctcttgact atgctaatga
     1381 gatcatttca ggatttagag agtgctacat atttcttcta tcacaacgtt caaagattaa
     1441 aggaatagta gaaggttttc cagaattgaa aagcagagcc ctatttcgaa atacttccga
     1501 ctatggaaaa tttttgcaag cttctacgaa tccaaagtat cttttctcag aaaaaaagag
     1561 gaagaattta ttttcgatac tatatgaggc aaaacatatt gaacatttta ttgttgataa
     1621 tgaaattaaa gatttgatga atggtgatat tccttacttt tcaatggata ccagaggaaa
     1681 cgtctataat tctgtaggga cattaattgg aaatctagga gatacaacct cgttatttga
     1741 tagtataact attttgaacg atgaaagatt gaagttcact tgtgaattgc ttgaaatagt
     1801 ctagaaaaaa ccgattaagc attgggagag ggaaaagggc aaatcttatc agtttttatc
     1861 aatctcctca gaacacaatt ttagtgagga aatcttagac agcattagac gaatatttat
     1921 tgatgcagat aagaatagct tttcttcaga ggaggagata acatggctca atattgatat
     1981 tacagagact gagcaatggg taatatctcc tcagaacatt accttctcta aaattgataa
     2041 aactgatttt aggaatatat cggcttttgt atctcaagat tctccaatat ttgatgggga
     2101 tgtgatgtat aacatttcgc taggaagaga atctgttaca gaaaaacaag ttattgaaac
     2161 ctgtaaaagg gtatcactat atgaggatat caggagtatg ccaatgaagt ttcatacccc
     2221 actttttcga gacaatccat cactatctgg ggggcaaaaa caacgaattt ctctagcaag
     2281 agagttagta actaccccta gaatcttagt tcttgatgaa cctacatcag ctttagatgt
     2341 aaaaactgaa agaataatcc aaaaaaatgt tgaggcttta cattgtacga gggttttggt
     2401 tacccataga cttaatacag ttgaaaaagc tgataagatt ttaataatgg ataatgggaa
     2461 aattattgac tatggtagtc atcattgttt atataaaaat aatgagtact atcgtgattt
     2521 atatgattcg tacatgaaca actatcagga ggaagggata aaatgaatca attactattg
     2581 aaaaatgttt gcaaaaggta tccaaaccaa ccgaattatg ctttagacca tatcaatttg
     2641 actgttagaa aaggagaatt tgtagctgtc atgggacgta gtggtagtgg aaaaacaaca
     2701 cttttaaatg ttacttcaat tattgataaa attgatagtg ggagtattta ttgtgcagac
     2761 aaagaaatta gtgcattttc tgataacgag gcaactagtt ttagaaaaaa tgatattggt
     2821 tttgtttttc aagattatat gctgttagac agtctaacca ttagggagaa tatttctgtt
     2881 gctctatctt taaaaaatgt tgattcatca aaaattaatg atttgataaa taactatgca
     2941 aaaagattta atttatatga gcagttggaa aaatatccgt atcaactttc tggaggtcaa
     3001 agacagaggg tcagtattat cagagcaatc attaaagaac cagagattat ttttgctgac
     3061 gaaccaactg gagcacttga cttgaaatct tctgaggaaa caatgatgat tttgagtgaa
     3121 atcaataaaa cagaaaaggt aactatttta atggttactc atgacgtttt atcagcttct
     3181 tatgcggata gagttgtact tctcaaagat ggtaaacttc acctggagat tgataaagag
     3241 gattgtggag agacgtttta tgacgtaatt agtcaagcct tatccgatag aggagagtaa
     3301 tatgatttgg tccattacaa aatctaacat caaaaaaaac ttttcgttat atcgtatcta
     3361 ttttctagct acgattggtt tattaagtat ttttatagct tttctaaatt ttatctcaga
     3421 taaaatcatt acagaaaaaa ttggggatag tggtcaagct ctagttattg ctaacgggtc
     3481 attagttttt ttgactgtat ttttggtgat atttttaatt tatttcaata atttctttgt
     3541 aaaaaaacgt agtcaagagc ttggagtctt agcaatacta gggttttcaa aaaaagaatt
     3601 aacacaatta ctgactttag aaaatcttgt tattctagtt ttaagttact tggtaagttt
     3661 actattggga ccgaccttat attttttagc ggtactggta attactcatc tattggaatt
     3721 aacattggaa gttcagtggt ttattacagc taatgagatt atagagtctt taggaatatt
     3781 agtcgtagtt tttctgatta atgtcatcac aaatggactt atcattagta aacagtcttt
     3841 gattgaattt gttaatttct caagaaaggc tgagaaaaaa attaagataa gaaaagtcag
     3901 agctattatt gctatcactg cattgctatt gtcatatatt ttatgtttgg cgacagtgtt
     3961 ttcatccaca cgaaatatgc tattaagcat agggatggta ccggtttctc tattgataat
     4021 tgtcttagtt gttttaggaa cagtgttcac catcagatat ggattggctt ttgtagtttc
     4081 gttattaaaa ggaaataaaa aaaggttata ccgtcctctg tctaatatca tctatcccaa
     4141 atttaactat cgtattgcaa caaaaaataa attattaaca gtcttgggag gtcttttaac
     4201 agtaaccgtt tcagttgccg gaatgatggt aatgctctat gcttattctc ttaatgggat
     4261 agagaggttg actccatctg ccatagaata taatgttgaa tcagaaaacg gtcaagtcaa
     4321 tgttacaact attttagaga acgaccaagt gagcttggtt gatgttgacc tattgagatt
     4381 gaacactaac ccagaagtaa ctatcacaga atctgggcaa acaattcctt attttgatat
     4441 aattaattac agtgattata aagagttaat gaaagctcaa gggagaataa attctattga
     4501 aggtagtaag tcactcccat tgttaataaa ttattatcca acagaaatta gccttggaaa
     4561 aacctttaac ttaggaaatg catatgatgt tactgtaaaa caagtatcaa cgcataatgt
     4621 ttttagtttt tctacaagtg tcacgacctt ggttgtttct gataaattat atgctaaact
     4681 tagttcttgt tttccagaga aagaaatgac aattaggact tttaatggaa attcgattag
     4741 gtcaagtgaa gcattttaca atcagtttag tatggttcct gatgttatca gtagttatag
     4801 taaggaatac acagtaaaga ctgctaatat tgcgacttat atctttataa ctttcctatc
     4861 catactcttt attatttgta caggtagtat tctgtacttt acaagcctca tcgaaatcat
     4921 ggaaaataaa gaagaatatg gctatataag taagctaggt tatagtaaaa aaatgattca
     4981 tcggattctt cgatatgaaa caggtatact tttccttatt cctgtattca ttgggattgt
     5041 aaatggtggt atgttgctta tttactataa atatttattc atggatacat tggtagcagg
     5101 caatatcata atgttatctt tattgctttg tctgcttttc ttcttgataa tatatggcac
     5161 attttatgta ttgacattgc ggtcagtgac atccataatc aaaaattaat tttaaaaatc
     5221 ttttaacttt aacgattaag taaataatat gataagaata aaaaacataa caaagagtaa
     5281 gttctttggg actgcaataa tcttgttgca acaattaata gccttgttaa ttttggtcta
     5341 taatcgagag aacctgtctc ttcttttctc tgaaaaagta gctcttgtaa tgacccttat
     5401 tgatacagct tttatttggc tggcaacaat attacgccaa aaacaaggtg atatttttaa
     5461 aaggattatt tttatcatct ccttaacaat ttggcaatac ttagtatctg tgctgacaag
     5521 aggaactcct ctctttttaa gcagtggact acagat
//
LOCUS       DATURV010000073         5459 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DATURV010000073 DATURV010000000
VERSION     DATURV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.073"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..765)
                     /locus_tag="VCF75_001460"
     CDS             complement(<1..765)
                     /locus_tag="VCF75_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HEP2547012.1"
                     /translation="MRKYDIQKLLLSGENQSVEFKEAKNSFPKDGMKTICSFANTNNG
                     LLILGVSENTKNKDFYISGVNDSDKVLDELYSLLNNPNKINRNVINEESVEVTNVKGK
                     EIIIIPVNKVDYKDKPIYLNDNIASTYFRQGTGDFRCSQEQINAMLRDSAKESFDSTL
                     VQNFSISDLDRETINRYREKFDDINAEHPFSKLDTEQFLLKINALRRDRTDNKIKPTV
                     AGLLIFGTHNAIKEFIPHYNVEYVLKDFTENNRFKDR"
     gene            1322..1954
                     /locus_tag="VCF75_001461"
                     /pseudo
     CDS             1322..1954
                     /locus_tag="VCF75_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411913.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2049..2300)
                     /locus_tag="VCF75_001462"
     CDS             complement(2049..2300)
                     /locus_tag="VCF75_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547013.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     GLVQNKGRQKVWSLAGLVLAAIAVAVVAYAYYDPTVIPY"
     gene            complement(2534..3094)
                     /locus_tag="VCF75_001463"
     CDS             complement(2534..3094)
                     /locus_tag="VCF75_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547014.1"
                     /translation="MKTKSKRFLNLATLYLALLGTTLLMGRPVNADNADMDGYGSESK
                     VQREQSPYEKGVRDGHDAGYKQGKQDRQQGSPDASPTPPDKLRVPEPESNPYNKDHDK
                     QNNQRYKNGWDIAYRSSYYTGWYSAEGTGHLESDDYPNEEEGEDISDNSPSQGSTGST
                     DDGFSNLIDMVVDITTFLWGLVSNWF"
     gene            3794..3931
                     /locus_tag="VCF75_001464"
     CDS             3794..3931
                     /locus_tag="VCF75_001464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547015.1"
                     /translation="MLTYHELKKAVANRYIKGDMISITRKLVSSLILSFLIELYEYQE
                     F"
     gene            complement(4223..4876)
                     /locus_tag="VCF75_001465"
     CDS             complement(4223..4876)
                     /locus_tag="VCF75_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547016.1"
                     /translation="MKKTLTLLLALFAIGVTSSVRAEDAIKWELDPETSQNSPIAVNV
                     DNANKLRFTLPTGWSVSFKRKKDHYNPLEVNNKLVSETKNRNTSPRIVDYGKNGNPIL
                     AIYNDNQGSSGLPITVTVDKPNEGDELTLLYRADDGFFGGFTKLKDSKTLKKEKKEKE
                     RQLAEEKQIEEERKHVEQKQLEESMLKQIREEDHKPWHQRLSESIQDQWWNFKGLFQ"
BASE COUNT         1658 a         1057 c          945 g         1799 t
ORIGIN      
        1 tcgatcttta aagcgattat tttcggtaaa atcttttaaa acatattcta cgttatagtg
       61 aggaataaac tcttttatgg cattatgtgt tccaaaaatc agtaatccag ccacagttgg
      121 ttttattttg ttgtctgtac gatctcttct tagagcgttt atttttaaga gaaattgctc
      181 ggtgtctaat ttggaaaaag ggtgttcagc attaatatca tcgaattttt ctctataacg
      241 gtttattgtt tctctatcta gatctgatat agagaaattc tgaacaagcg tactatcaaa
      301 actttctttt gcagaatctc ttagcatcgc attgatttgt tcttgtgaac acctaaaatc
      361 accagtcccc tgacgaaaat aggtagatgc tatattgtca ttaagatata ttggtttatc
      421 cttgtaatct actttgttaa cagggataat gataatctct tttcctttta catttgtgac
      481 ctcaacactt tcctcgttaa tcacatttct attaatttta ttagggttat taagtaaact
      541 atataattcg tccaatactt tatcggaatc attaacgcca gaaatataaa aatctttatt
      601 cttggtgttt tctgagacac ctagaattaa cagaccatta tttgtatttg cgaaggaaca
      661 aattgttttc ataccgtctt taggaaaaga atttttagct tctttaaatt caacactttg
      721 gttctcccct gataataaca atttttgtat atcgtatttt ctcatttttt ctccaattaa
      781 ttcgcacacg cattcgcaca cacgtgggat aataaatttg tttcctgttc gaaaaaggcg
      841 ttattaaagg atttattgat aacatcaatt cacacacgca ttcgcacacg cgtgggaacg
      901 tctaaaaatc atctgagtta atttattctt gttttactta taacttctgc cagttattgt
      961 tataccattc ccaaacggca tctcttggga aagctaacct tcttccatat cctctatcaa
     1021 tctgctgtga gatatcttaa cgttctaaac tctccttttt cttcatcaag tataacattt
     1081 tttagcgtaa taaataaaaa aagtacgaaa aagcatcaaa aaagcctacc gactgcaatc
     1141 agagaggctt taagttcaaa atccaccaac aggcaagata ggaactttgg tcagcctgtc
     1201 tgcccctacc aaggatagtc gcgccatttc ccaaccacaa gacatgaact ccctatcttt
     1261 ttagcgcaag aattggtata ataacccttg tcttaaaaaa tgagcaaaag gagagggaaa
     1321 aatgaaaaag acattaacct tgctactggc actctttgcc atcgggataa ctagtagcgt
     1381 caaagcggag gaggtatttg aatttaaacc tatcggtcaa caaacagtac caacagttac
     1441 agtctcacct tttaaaacag gagagcaaac tattgacatc accctccaat caaacggtta
     1501 cgtatatgct tctataaaac ataaaaacgg agaaacacaa aaaattaaaa caaatcttaa
     1561 ataaatcact gttacatgtc cagcagagca aagcacctgt actgagcgta cttggatgat
     1621 aagcggaaat cgtgctgata aatatgggaa ttataccgtc caactacaaa aaccacttgc
     1681 agttggagac gtagttactc tatcctttgc aaatgatggt catttttact tgggtaaact
     1741 agtcttcgaa gatgaaaaaa cacaaaacac aaggttgcaa gcggaaaaag cacaggaaca
     1801 agaagaggca aaaaaacgcg ataatgaatt aagaaaacaa ctagaagaaa gcatgctaaa
     1861 gcagataaga gaagaagacc ataaatcttg gcatcagcgg ttaagcgaaa gcatccaaga
     1921 tcagtggtgg aactttaagg gactgtttca gtgagggttt acgaggctat gaggttggct
     1981 aaagcacaaa aagcacctgt caggggtgct ttttgtggtg ctgattgcgt gcgaagggga
     2041 ggttaaggtt agtaggggat gacggttggg tcatagtagg cataggccac tactgccacg
     2101 gctatagcag ctaagacgag cccagctagc gaccacacct tttgcctgcc tttgttttgc
     2161 actaggccga taagagctag actacttgct acaagcggca agtagagata actataagta
     2221 gcaaaaaaga agatatgatc aagcaggcag acaaaactca ccaccatgct taagacgggc
     2281 aacactttct tttctggcat acatgctcct ttctcaacac acgggtcact acaacctcaa
     2341 aaaaggccac ccctatcaga ggtggcctga ctactaaagg gactatccca attatagcag
     2401 attagtgtgg gaatggcttg agctaaggaa agtatcctca aattaagtat ctcccctgtt
     2461 atttaaaaat aatttattta gtatattatt tctcggtaaa tctaagctac gaagctcaaa
     2521 ctaaagtatc attttaaaac caatttgaca ctagacccca aagaaatgtg gtaatatcaa
     2581 caaccatatc aatgagatta gaaaaaccgt catctgttga acctgttgaa ccctgtgaag
     2641 gagagttgtc agaaatatct tctccttcct cttcatttgg ataatcatcg ctttctaaat
     2701 gacctgtccc ttcagcagaa taccagccag tataataaga agagcggtac gcaatatccc
     2761 agccattttt gtatctctgg ttattctgct tatcatgatc cttattataa gggttactct
     2821 caggctcagg tactctaagt ttatctggag gggtaggaga agcatctggg gatccttgtt
     2881 gtctatcctg tttgccttgc ttataacctg cgtcatggcc atctctcaca cctttttcat
     2941 atggactttg ttctcgttga acttttgact cacttccata tccatccata tccgcattat
     3001 ccgcatttac gggacgtccc attagcaaag tcgttcctag tagggccaag taaagggttg
     3061 ctagatttaa aaagcgtttt gattttgttt tcataaagtc actccatttc attatttttt
     3121 gacacggtta tagtttacta ctcgcattct ttcatttcaa gttcattttt ttatcatttt
     3181 aaatatcaaa tgattagaaa aaaatggcaa gctatcctta tcacagaaag tgcccgcttg
     3241 gagcagttag ataacctttt tgagctttag gaactgtttt tgaaagtatt tgatagttta
     3301 tataagaggg agggactttg catttgataa ggaataaccg ttgggcatag gccactactg
     3361 ccacagctag agctgctaaa acgagcccag ctagcgacca aaccttttgc ctgcctttgt
     3421 tttgcaccag actaataata gctagactac ttgctacaag cggcaagtaa agataactat
     3481 aagtagcaga aagaagatat gatcaagcag ggacacaagg ctcactacca tgcttaatac
     3541 gagcagccct ttcttttctg acataaaaat acctcttgag tagattttgg ttatttagcc
     3601 tgtctactta agacgtatca tatcatcaat cgagggcgat ttttttgttt ttgcacacac
     3661 aaacatcaaa catcttgatt taaaatattt acccgaacat gtattcgcac acgggcatat
     3721 ccgttataat ggactttttt taaatatcta ttttaagaga ttttttacga atagattaag
     3781 taggaggata aaaatgttaa catatcacga attgaaaaaa gctgtagcta acaggtatat
     3841 caaaggagac atgatcagca ttaccagaaa actggtaagc tctttgattt tgtccttcct
     3901 gattgagctt tatgaatatc aagaatttta gtatctttaa ttaaaacatg attagggata
     3961 ctgtccacga acaaactaat cagtcttgct tcatgcgtta tcaggatatc aaaagcaaga
     4021 tttaattcca aacttaattg atttgtgtta tactctttat acatcgtcat ggctttctag
     4081 ttgttttgcc gttattataa ttataaacca tggcagaaga aaacgagcat ctccgactgc
     4141 ggaaatgctc gttttttata tgctaaagct agtttttgcc tagcctcttg tgctttagcc
     4201 aacctcatag cctagcaaac cctcactgaa acagtccctt aaagttccac cactgatctt
     4261 ggatgctttc gcttaaccgc tgatgccaag gtttatggtc ttcttctctt atctgcttta
     4321 gcatgctttc ttctagctgt ttttgttcaa cgtgctttct ctcttcctca atttgttttt
     4381 cttctgctag ctgtctttct ttctccttct tttctttttt tagtgtcttc gaatctttta
     4441 attttgtaaa tccgccaaaa aaaccatcat cggcacgata aagcagtgtt aattcgtctc
     4501 cttcatttgg tttatcgaca gttacagtga ttggtaaacc tgatgaccct tggttatcat
     4561 tataaatagc taaaatagga ttaccatttt taccataatc tactatccta ggactagtat
     4621 ttctgttttt tgtctcagat actagcttat tatttacctc tagaggattg taatgatctt
     4681 tttttctttt aaaagatacc gaccagcctg taggtagtgt gaaccgtagt ttattagcgt
     4741 tatcaacgtt cacagcaatt ggactatttt gagaagtttc tggatctagt tcccacttga
     4801 ttgcatcctc cgctctgacg ctactagtta ccccgatggc aaagagtgcc agtagcaagg
     4861 ttaatgtctt tttcatttca ttctctcctt ttgctcattt tttaagacaa gggttattat
     4921 accaattctt gcgctaaaaa gatagggatt gattggctga ggcgaccgga gaagtccttg
     4981 atctctctga gtagctgctg ctcacttaat gtttcactat tttattggga tatgctaata
     5041 cttcaagata tctttatatc gtctatagtc aagagcctat ttgacacttt atctctatta
     5101 aataatttgc acactctgtt aagtgctctt tttggtcgct tattctctct taaagttgtt
     5161 ttatgtcagt tgaaaacgac gtttcttgag taacctaact tacctgatat ctcgtatcgt
     5221 gatatgttat ttatcaatat tatgaaatag aacaacgata ttaggcattc ttttcaatta
     5281 acttcgaata gtttaataaa tgtttatcat ctgcagtttt tagcataatt taaagaaata
     5341 cgactgtatc ttgataggta ctgttttttg atgtctttaa caatatattc tcaaattttc
     5401 aagaataact accttgctca aagatatatt ttatattttc ccgcgttgta aaatgaagt
//
LOCUS       DATURV010000074         5436 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DATURV010000074 DATURV010000000
VERSION     DATURV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.074"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(113..1255)
                     /locus_tag="VCF75_001466"
     CDS             complement(113..1255)
                     /locus_tag="VCF75_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEP2547017.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFCQTTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILAANLMLLIAQFNK"
     gene            1536..4514
                     /locus_tag="VCF75_001467"
     CDS             1536..4514
                     /locus_tag="VCF75_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEP2547018.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDSQTTSAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVVTTIQKLNAMISQMESYDTPKFKKLKERLEHLNVV
                     FVVDECHRAVTPERQRHLTNTFRNSRWYGFTGTPIFMENKRAQLGDLAQTTERQYGKC
                     LHQYTVKEAIHDKSVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLNSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLIQKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFLEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAYQAFDKLYASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIAYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            4527..>5436
                     /locus_tag="VCF75_001468"
     CDS             4527..>5436
                     /locus_tag="VCF75_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP2547019.1"
                     /translation="MTKSKQPQYRFDGFEGEWEEKRISDIANVFTGGGTPSTQNDQYW
                     TGNIPWIQSSDLSKDEILDVNPKKFITKLGLSNSAAKLIPKNSIAIVTRVGVGKLSII
                     NREYTTSQDFLSISDLKENLKFTVYSLYRLLQKELNQLQGTSIKGITKGYLLSKTILI
                     PSLPEQGAIGELFQMVDQLIQLQDQKLATLKEQKQTFLRKMFPAQGQKVPEIRLQGFK
                     GEWEEKKLGEVSTHRSGTAIEKYFDSEGEFKVISIGSYGTNNLYVDQNIRAVSNELTN
                     SKLVASGELTMVLNDKTANGAIIGRCL"
BASE COUNT         1817 a         1079 c          998 g         1542 t
ORIGIN      
        1 acaaaatgtt tctgtttttc atactatgct aatgagttag ctcgtgcgtc tccttcttca
       61 taggttactt ttaaccgcca aatgaatgct acacaatcga aaatagatga gtttacttat
      121 taaactgagc tatcaatagc ataaggttag ctgctaatat tttgatatta gaaagtgttg
      181 ttgttttatc tatcgctgct tctaaggaaa ttggctgctg ttgtattgaa aaaacagcca
      241 aaaagtcttc tgctgctagt cctgtctcaa tctttacaga accacataga gcaatcgttg
      301 gaacttgata ttttttagcc attcttgcaa tcgcaacagg taccttgcca gattgtgatt
      361 gtgtatccag gcatccttct ccagtaataa ctaaatcaca agactgaagg gaattatcca
      421 gatttagtag ctctgcaatc ctgctaaaac cactagttaa ggtgcctcct aataggacaa
      481 tggcacctcc taatcctcca gctgctccac ttcctggtat tgtctgtaaa tcaatggttg
      541 tttggcaaaa aactttttta gcaaagttac ttgcaatttg atcagtttct tcaatttgac
      601 taaggctccc acctttttga gggccaaaga ccgcagcaaa tccttgaggg ccatgatatg
      661 gattggtcac atcagtcagc cctaatagcg taacaggtga tgctaaagta tcaagataag
      721 atcttcctgt catgaaatca tagtttaaac tttccaaaaa ccctttgcca ccatcagaag
      781 tgcctgttcc tcctagcatg atttcgattt gagtagctcc tttttgaatg gcatctttaa
      841 ctgccaaacc caaaccatag gaagtggctt gtgcataggt tactgaattg ggggtgatct
      901 tatcaatacc aataatactc gcagattcaa taaaagcttg ctttgcgtga cgataataag
      961 cgactttaat cgggcgtctt aatagatcaa tggtttttac ttgatgccac ctccccgcca
     1021 cggtttgtga gagggctgct aaacttcctt ctcctccatc agcgatagct cttgtttcaa
     1081 taactaactg cttatcgaca gaaagaaggg cttgagctac actagtgtta agttctggag
     1141 acgttaccga tcccttaaaa gaatctatgg ctaccaaaat tttcatatct tcaataaatt
     1201 ccttttctgt atattgtagc agattatcgt cctttaaaac cctattaata gtcaagctag
     1261 atctgtctta agttttttat ctattttgag aactcgacct ttttactaat gttgtcttat
     1321 tactttcttc accttaaatt attgtcaagc aaactcaaaa atacaagaac tttctcgatt
     1381 gcatttacat agtcatagct ttgtttagtt gctaggatct taagcattca actaatcaaa
     1441 aaaagactgt ttggaaggtt tcaatagctt tgccattcta aaaaggatcc gccaatgata
     1501 taatattact atatcatgtc gaatggggat taaaaatggg aaatcaaact aaaacggaac
     1561 ttgaacttga aaaagagttg atccatctct tagaaacagg tgaaagtcaa tggacatatc
     1621 gtaaagaatt aaaaactgaa gatgctttat gggataattt ctttaagatc ttagcgcaaa
     1681 ataacacgca atatctgaac gaggagcctt taacagctag tgaaaaagag cagattaaga
     1741 atcaacttaa ctttgtcaat tactatgaag cggctaaatg gttagctggt gaaaacggca
     1801 ttgctaaagt acaagtgcaa cgcgaagatg caaaactagg aacgattcgt ttagaagtcg
     1861 ttaaagcaga caatgtggct ggtggaactt ctgtttatga gattgccaat caagttgctt
     1921 tctctggttc tcgtgataga cgaggtgatg tgacacttct catcaatggc ttacctatga
     1981 tccaaattga attaaaatct cagaatcatc aatgcattga agcttttaac caagtcaaaa
     2041 agtatgataa agaaggccag tttagaggta tcttttcaac cttacaaatg tttgtggtct
     2101 caaataaaac agatacaagg tatatcgctg cagctaaaga aaataaatta aaccctaact
     2161 tcttgaccca atgggtagat caaaacaaca aaccacaaaa agatctcttt gcctttgcca
     2221 aagaagtcct atctatccct cgtgctcacc aaatggtcat gacttactct gtcattgatg
     2281 atgataaaaa agcactaatt ctccttcgtc cttatcagat tcatgcaatc gaagccgtcg
     2341 ctgaagcgag tcgtcaccgc aagtctggct atatctggca cacaacaggt tctggaaaaa
     2401 ccttaacttc ctataaagtg gctcgcaata tccttcaaat accggccgtt gaaaaatcca
     2461 tctttgtcat tgaccgaaag gatttagata gtcagaccac ttctgctttt caatcttatg
     2521 cgcaaaatga tatctttgat gtcgatgaga cagaagatac gagacaactc atcaaaaatt
     2581 tagaatccag tgatcgtcgt gttgtggtca ccaccattca aaaactcaat gctatgatca
     2641 gtcagatgga aagttatgac actccaaaat tcaagaaact aaaagagcgt cttgaacacc
     2701 taaatgttgt ctttgttgta gacgaatgcc accgtgctgt cacgccagaa cgtcaacgtc
     2761 atctcacgaa tacttttcga aacagtcgct ggtatggctt tacaggtact cctatcttta
     2821 tggaaaataa gcgtgctcaa ttaggtgatt tagcccaaac aacggaacgg caatacggca
     2881 aatgtctcca tcaatacact gtcaaagaag ccattcacga taaatcggtt ctaggcttcc
     2941 aagttgagta taagacaacc attcctgata tgcctgaaga cagtattcct gaagaagctt
     3001 atgatcatga agaacatatg ttagctgtct taaatagtat cattaatcaa tcacgtaaga
     3061 agcttggctt taataatggg attggtcaga cattcgaagg cttattaacc gttaagagta
     3121 ttgctcgtgc ccaagcctat tatgatctga ttcagaaagt caaagctggt gaaacggatt
     3181 tagtcatttc taagaaggtc aaagaaaaac tgccagactt tccaaaggta gctattactt
     3241 attctatcac tgaaaatgac aatgcgtcta tcagccgaca agataaaatg accaaaaatc
     3301 tagaagacta taatcacctc tttggcacta actttaccat cgataactta caaggctata
     3361 acagagattt aaatgatcgc ttagcccgta aaaaagataa attcaaagac cgtcatgaac
     3421 aattagacct cgtgattgtg gttgaccgtc tcttaacggg ctttgatgcg ccttgtttat
     3481 caaccatctt catcgatcgc cagcctatga aacctcagca cattattcaa gctttctcac
     3541 gaaccaaccg tatctttgaa agtagaaaac attacggtca ggttgtgact ttccaaacac
     3601 cacttcgttt taaagaagct gttgacaaag cgcttagcct ctactctaat gggggtgaaa
     3661 acgacgtttt ggccccttct tgggaagaag aaaaagcaag attccttgaa aaagtaacag
     3721 tattaaaaaa tatagttcct gatccagacg ctttcccaac tattgagagc gcacagacag
     3781 cttttctcaa acaatacgcc aaagcctatc aagcatttga taagctctat gcatctgtac
     3841 aggtctatag tgactttaat gaaacactgt tgagtgaggt tgggttatca gatgaagtga
     3901 ttgatactta taaaggcact taccaaaatg tcattgcaga aatacgaaag cgaagagaag
     3961 atgacgaagc catcccagaa atcaatatcg actatgagct agaaagtgtt cagatggatg
     4021 atatcaacta tcactatatc ttgaccttaa ttcaagcctt tgtcgatcaa gaacaagaag
     4081 ctcttcaaga aaggctcaat gacaatccca tggatcaata catccaagat ttggcaaaat
     4141 caaaccctgc tatggctgac tcactagctg agttatggca agatatccaa aaagaaccca
     4201 aagcctatga agggaaaagc attgcttatg agttggataa cctcattggt gataaaatcc
     4261 aaagggccat caaacacttt gcggaccaat ggaaggcaga tcctgacaaa ttagcctttg
     4321 tggcgactaa ttatcatagt gctaactcta ccaaacaagt gggcatgtca accttaaaag
     4381 aatccctaga ctaccaagcc tataaagaaa aacaaggtga ttctgccatg aataaactca
     4441 aatacaagag ccaatttgaa cgggagttag ttcaattcat tagagatcaa atccaaccct
     4501 taaaagggca ctaggaaagg aaagagatga caaaatctaa acagcctcaa tatcgctttg
     4561 acggttttga aggggagtgg gaagagaaac gaatttctga tattgcgaat gtgtttactg
     4621 gaggtggcac acctagtact caaaacgatc aatactggac tggcaatatc ccctggatac
     4681 aatcatctga tttatccaaa gatgaaatat tagatgtaaa ccccaaaaaa tttattacaa
     4741 aattgggcct ttctaactct gctgcaaaat taataccaaa aaattctatt gctattgtta
     4801 cacgagttgg tgtaggaaaa ctttcaatta tcaataggga atacacaacc agccaagatt
     4861 ttttatctat ctcagatctt aaagagaact tgaaatttac tgtatattcg ctatatagac
     4921 tcttacaaaa ggaactaaat caattacaag gaacgtcaat taaaggtatc acaaaagggt
     4981 atctcctttc taaaacgata cttatcccct ctctccccga acaaggagcc attggcgaac
     5041 ttttccaaat ggttgatcaa ttgattcagt tgcaggatca aaagctagca acccttaaag
     5101 aacaaaagca aaccttcctc aggaaaatgt tcccagctca aggacaaaaa gtccctgaaa
     5161 tccgtttaca aggttttaaa ggagagtggg aagagaagaa attgggggaa gtatctactc
     5221 atagaagtgg aacggcaatt gaaaaatatt ttgattcgga aggtgaattt aaagtcattt
     5281 caataggtag ttatggaact aataacctct atgtagacca aaatattaga gcagtctcaa
     5341 atgaacttac taattctaaa ttagttgcct ccggtgaatt aacaatggta ctcaatgata
     5401 agacagcgaa tggggcaata atcggaagat gtctac
//
LOCUS       DATURV010000075         5257 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DATURV010000075 DATURV010000000
VERSION     DATURV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.075"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            27..548
                     /locus_tag="VCF75_001469"
     CDS             27..548
                     /locus_tag="VCF75_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP2547020.1"
                     /translation="MTKDRQIKKTKTAIYSAFIALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLELIR"
     gene            763..1281
                     /gene="rimM"
                     /locus_tag="VCF75_001470"
     CDS             763..1281
                     /gene="rimM"
                     /locus_tag="VCF75_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEP2547021.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKFKDMYHINAIEKYKGYTLKVSKANQGDLQEGEFYYH
                     QIIGMAVYEKGRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            1271..2002
                     /gene="trmD"
                     /locus_tag="VCF75_001471"
     CDS             1271..2002
                     /gene="trmD"
                     /locus_tag="VCF75_001471"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEP2547022.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALGQGED"
     gene            2002..2994
                     /locus_tag="VCF75_001472"
     CDS             2002..2994
                     /locus_tag="VCF75_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP2547023.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGAELTENLIKQLSRFEDHTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKASTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEMLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            3172..4230
                     /locus_tag="VCF75_001473"
     CDS             3172..4230
                     /locus_tag="VCF75_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEP2547024.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFED"
     gene            4243..5166
                     /locus_tag="VCF75_001474"
     CDS             4243..5166
                     /locus_tag="VCF75_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEP2547025.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     VVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDEGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
BASE COUNT         1657 a          847 c         1128 g         1625 t
ORIGIN      
        1 tgaacaacat ttagaaagtg tccgatatga caaaagacag acaaatcaaa aaaacaaaaa
       61 cagctatcta tagtgctttt attgctttat tacaaaaaaa agaatattcg aaaataactg
      121 ttcgagatat gattacactt gctaatgtag ggagatcaac cttttatgca cattatgaaa
      181 gcaaagaaat gttactaaaa gaactctgtg aagaactttt tcatcatttg tttaggcaaa
      241 aaagaaatgt gacttttgag gactatttgg tacatatttt aaagcatttt gaacaaaata
      301 aagacagcat tgcgacgttg ttactttcta atgatcccta ttttttatta agatttaaaa
      361 acgaactaga acatgatgtt taccctaact tgcgctgcaa gtatatcgac aaaacgacta
      421 ttccagaagt attcctgaaa caatttgttc tatctagttt tatagaaacc ttaaaatggt
      481 ggctccatca aaggcaaagg atgtctgcaa atgaactatt aaagtattat ttagagctca
      541 taagataatg actagtgagt catcttgcca aattcaattt tttaatacag acggatttta
      601 acaatttaac agagaaagac aaaactgtaa gtgcaaaata gactataacg tgataaaata
      661 ataggtaatt tggttctgaa aaagaagtgt ctatcaaact ttagaatcaa gagaggtcgc
      721 taaagtgacg tgagataaag agttaacgat ttaggagcta ccatggaata ttttaatgtt
      781 ggaaaaattg tcaacacgca aggcctgcaa ggagagatgc gcgtgttgtc tgtcagtgat
      841 tttgctgagg aacgctttaa aaaaggctca caattagctt tatttgatga taaagatcgg
      901 tttgttcaag aagtgacaat tgttagtcac cgtaaacaaa agcattttga tattatcaaa
      961 tttaaagaca tgtatcatat taatgcgatt gaaaaatata agggctatac gttaaaagtg
     1021 tcaaaagcta atcaaggaga cctgcaagaa ggagaatttt actaccatca gattatcggt
     1081 atggctgttt atgaaaaggg ccggttgatt ggctatgtca aggaaatctt gcagccaggt
     1141 gctaatgatg tttgggtagt gaaacgccaa ggaaaacgag acttgctctt accatatatt
     1201 cccccagtag ttcttaatgt agatgttcct aacaagcgcg tggatgttga attgatggaa
     1261 ggcttagacg atgaagattg atattttaac cctttttcct gaaatgtttg cccccctaga
     1321 acattccatt gtaggcaaag caaaagaaaa aggattactt gacattcact accataactt
     1381 tcgagattat gctgaaaaag ctcgtcatgt ggatgacgaa ccctatggtg ggggccaagg
     1441 catgttattg agagcacagc ccatttttga caccattgaa cagattgaag ccaaaaaacc
     1501 aaggattatt cttttagatc cagcaggtaa accctttact caggcttatg ctgaagaatt
     1561 agctcttgag gaagagttaa tttttatttg tggtcattat gaaggttatg atgagcggat
     1621 taagaccttg gtgacggatg aaatttcatt aggtgacttt gtattaacag gaggtgagtt
     1681 ggcggccatg actatggtgg atgcgaccgt caggttaatt ccacaggtac ttgggaaaga
     1741 aagtagccat caggacgatt ctttttcatc aggactttta gaataccctc aatacacaag
     1801 accttatgat tatcgtggta tgacagttcc agatgttcta atgagtggtc atcacgaacg
     1861 tattcgcttg tggcgtctag aagaaagtct cagaaaaacc tatttgagaa ggccagattt
     1921 gttagagcgg tacgattttt ccgaggaaga gcgcaagcta ttggataaga ttaaagaagc
     1981 attgggtcaa ggagaagatt agtgaaagat aaagcatatg atattacgat tattggtggt
     2041 ggtccaatag gtttatttgc agccttttat gcaggtctta ggggcgtaac agtaaagatt
     2101 attgagagtt tgtcagaact tggtggacag cctgccattc tttatcctga gaaaatgatt
     2161 tatgatattc cagcttatcc atctttaaca ggtgctgagt tgacagagaa tttaataaaa
     2221 caattaagcc gttttgaaga tcatacaacc atttgtttaa aagaagaagt attaaccttt
     2281 gacaaggtta aaggtggttt ttcgattagg actaataagg cagagcattt ttcaaaagcc
     2341 attatcattg cttgtggaaa tggcgctttt gcaccaagaa ctttagggtt agaaagtgag
     2401 gaaaactttg ctgatcataa ccttttttat aatgttcacc agttggacca gtttgcggga
     2461 caaaaagttg tgatttgtgg tggtggagat tcagcagttg attgggcttt ggctttagaa
     2521 gatattgctg agagtgtgac ggttgtgcac cgtcgtgatg ctttcagagc acatgaacac
     2581 agtgtggaat tattgaaagc atcaacggtc aacctcttaa caccttatgt gccaaaagca
     2641 ttgaaaggaa ttggaaacct ggctgaaaaa ttagttattc aaaaagtgaa agaagatgaa
     2701 atgttagaac ttgaattgga cagtcttatt gttagttttg gtttttcaac ttctaataag
     2761 aatttgaaaa attggaatct cgactataag cgttcaagta ttactgtttc accacttttt
     2821 caaacaagtc aagaaggtat ttttgccatt ggtgatgcag ccgcttataa tgggaaagtg
     2881 gatttgattg ctactggttt tggcgaagca ccaacagcag ttaatcaggc cattaactat
     2941 atttatccag atcgagataa ccgtgtggtg cattcaacct ctttaattga ctaagggagt
     3001 cggaagtttc aagagcagta gcaacagcta atattcagtt tgtcaggaga ggacaaatgg
     3061 tttttaccta agtcttcgtg acaagtttta tactttgtga taaaatagtc attgtgaaaa
     3121 aagtgtgatg gttttcacag attaacacat tacaatgaaa aggggtctat catgacaaca
     3181 acagataaag agacatttag ttcttttatg aataaggtct tggctggtac agcgattgct
     3241 atcgttgtgg cactcattcc aaatgctatt ttagcgactt tcttaaaacc gcttttgcca
     3301 aacatggcag ctgctgaatt tttacacatt gtgcaagtct tccaattctt cacgccgatt
     3361 atggctggtt tcttgattgg gcaacaattt aagtttaatc ctatgcaaca gttggctgtt
     3421 ggtggagctg cttatatcgg ttctggggct tgggcttata cagaagtcat tcaaaaaggt
     3481 gtggcgacag gaacattcca acttagaggt attggggact taatcaatat gatgattaca
     3541 gctagccttg cggttttagc tgtgaaatat tttgggaata aatttggctc tttaaccatt
     3601 atcttgttac ctatcactat aggaactggc gtaggttata ttggttggaa atttttgcct
     3661 tatgtgtctt acgtgacaac cctgatcgga caaggtatta attcttttac aaccttacaa
     3721 cctattttaa tgtctatttt aattgcagtt gctttctcgc ttatcattgt tagtccgatt
     3781 tcaactgttg caatcggttt agctattggg cttaatggaa tggctgcggg agcagcttct
     3841 atgggaattg catcgacagc agcagtctta gtgtgggcga cgcttaaagt taataaatct
     3901 ggtgtaccaa ttgctatcgc acttggggct atgaaaatga tgatgccaaa cttcttgaaa
     3961 catcctatca tggctattcc gatggtgttc actgcagcaa ttagctcatt aacagtacca
     4021 ttgtttaatc ttgttggaac accagcttca tctggttttg gcttagttgg tgcagtaggt
     4081 ccgattgctt ctttagcagg tggtagctca atacttatta ttatccttgc ttggatcatt
     4141 gttccgtttg cggttgcttt tgcggcgcat aaggtttcta aagatattct aaaactttat
     4201 aaagaagaca ttttcgtctt tgaagactaa taggaggaaa atatgttagt ttatattgct
     4261 ggctcaggag ccatggggtg ccgttttggt taccaaattt caaaaacaaa taacgatgtg
     4321 attttgttag ataactggga agatcatatc aatgccatta aagaaaacgg tttagtggtg
     4381 acaggggatg tggaagaaac agttaagctt cctattatga aaccgactga agccactcaa
     4441 gaagccgatt tgattatctt gtttacaaaa gcaatgcagt tgccacaaat gcttcaagac
     4501 attaaaggca ttattggaaa agaaaccaaa gtgctttgcc ttttaaatgg tcttggacac
     4561 gaagacgtta ttcgtcagta cattccagag cacaacattt tgatgggggt cactgtctgg
     4621 actgctggtc tagaaggacc aggtcgtgct catcttcaag gagttggtgc tcttaactta
     4681 caaagtatgg atccaaacaa tcaagatgag ggacatcaag tagctgactt gctaaataaa
     4741 gctaacttaa atgcgactta tgatgagaat gttgtgccaa acatctggcg aaaagcttgc
     4801 gtgaatggta ccatgaattc gacttgtgct cttttagatt gtacgattgg cgaattattt
     4861 gccagtgaag atggtctcaa aatggtaaaa gaaatcatcc atgagtttgt tatcgttggt
     4921 caagcagaag gtgtcgaact aaacgaagaa gaaatcacgc aatatgtaat ggatacatct
     4981 gttaaagctg ctcaccatta cccatcaatg caccaagacc ttgtgcaaaa tcaccgattg
     5041 actgaaattg attttatcaa tggtgcagtc aataccaaag gtgaaaagct tggtatcaat
     5101 actccatact gccgtatgat tacagaacta gttcacgcta aagaagcagt gttgaacatc
     5161 caataaacgc tatcaaatcg ttatactcaa tattatggct aaagctttaa tattgagttt
     5221 tttgttatgc tgatagtatt tttcttaggc gtcatta
//
LOCUS       DATURV010000076         5094 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DATURV010000076 DATURV010000000
VERSION     DATURV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.076"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            30..248
                     /locus_tag="VCF75_001475"
     CDS             30..248
                     /locus_tag="VCF75_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEP2547026.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            268..2427
                     /gene="nrdE"
                     /locus_tag="VCF75_001476"
     CDS             268..2427
                     /gene="nrdE"
                     /locus_tag="VCF75_001476"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP2547027.1"
                     /translation="MSLKDIGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSNLVKSLFKDHFIPQAEDWKALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            2760..3719
                     /gene="nrdF"
                     /locus_tag="VCF75_001477"
     CDS             2760..3719
                     /gene="nrdF"
                     /locus_tag="VCF75_001477"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP2547028.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINDIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            3694..5007
                     /locus_tag="VCF75_001478"
     CDS             3694..5007
                     /locus_tag="VCF75_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEP2547029.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKADQKLLLACASSAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWSVLAGLILSLFAHAYKHLLEHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
BASE COUNT         1517 a         1033 c          904 g         1640 t
ORIGIN      
        1 aaccattact aattttagaa ggacaagaca tgattacagt atattccaaa aataattgca
       61 tgcagtgcaa aatgactaag aaatttttag aacaacacgg agccaacttc caggaaatta
      121 atatcgatga acatcctgaa aaagtggatt atgtgaaaag tcttggcttc acatctgctc
      181 ctgttatcga agcagataac ctcgtatttt ctggcttcca acctgcaaag ttaaaagaat
      241 tgatttaata agacaaggaa aaataatatg agtctcaaag atattggcga tatttcatat
      301 tttcgcctaa ataatgaaat taaccgtcct gttaatggta aaattccact tcataaagac
      361 aaagaagctt taaaagcttt tttcgctgaa aatgtgctgc caaacaccat gtcttttact
      421 tccattacgg aaaaaattga gtatttaatc tcaaatgatt acattgaatc agcttttatt
      481 cagaaatacc gtcctgaatt tattactgaa ttagatagca taatcaaatc agaaaatttt
      541 cgctttaaat catttatggc agcctacaag ttctaccagc aatacgcctt aaaaacaaat
      601 gatggagagc attatttaga aaaccttgaa gaccgtgtct tgtttaatgc tttgtatttt
      661 gcagatggtc aagaagactt agcaaaagat ttagccgttg aaatgattaa ccaacgttac
      721 caaccggcta ctccttcctt tttaaatgct ggtcgaagcc gtcgtggtga attggtctct
      781 tgtttcttga ttcaagtaac tgatgacatg aattctatcg gacgttccat taactctgcc
      841 ttgcaattat cccgtattgg tggaggtgtt gggattacct tgtctaacct ccgtgaagct
      901 ggcgcaccaa tcaaaggcta tgctggtgca gcctcaggag ttgttcctgt tatgaaatta
      961 tttgaagata gtttttctta ttcaaatcaa cttgggcaac gtcaaggagc tggtgttgtt
     1021 tacctaaatg tttttcatcc tgatatcatt gctttcttat ctactaaaaa agaaaatgcc
     1081 gatgaaaagg tgcgtgttaa aaccttgtca ctagggatta ccgttcctga taaattctac
     1141 gaattagctc gtaaaaacga ggacatgtat ctctttagtc cttacaatgt tgaaaaagaa
     1201 tatggcattc cctttaacta tctcgacatt accaatatgt acgatgagtt agtggcgaac
     1261 cctaaaatta ctaagactaa aattaaagct cgtgatcttg aaacagagat ttcaaaatta
     1321 caacaagaat ctggttaccc ttatatcatc aatattgata cagctaataa agctaatcct
     1381 atcgatggaa aaatcatcat gagcaacttg tgttctgaaa ttttacaagt tcaaacacct
     1441 agccttatca atgatgcgca agagtttgta gaaatgggaa ctgatatttc atgtaactta
     1501 ggttccacta atatcctgaa catgatgacc tcaccagact ttggccgttc tattaagacc
     1561 atgacacgtg ccctaacttt tgttactgat tcatcaagca ttgaagctgt tccaaccatt
     1621 aaacatggca atagccaagc tcatactttt ggccttggag ctatgggact acattcttac
     1681 cttgctcaac atcatattga atatggcagt ccagaatcca tcgagtttac tgatatttac
     1741 tttatgctcc tgaattattg gaccttggtc gaatccaata acatcgctcg tgagcgccaa
     1801 actacctttg ttggctttga gaactctaag tacgctaatg gtagttactt tgataaatac
     1861 gttacaggac actttgttcc aaaatctaat ttggtcaaaa gtctctttaa agatcacttt
     1921 attcctcaag cagaggattg gaaagctctt cgcgacgccg ttcaaaaaga tggtctttat
     1981 catcaaaacc gtctagcagt tgctccaaat ggctctattt cttatatcaa tgactgctct
     2041 gcttctattc acccaatcac acaacgcatc gaagagcgtc aagaaaagaa aattggtaaa
     2101 atctactatc ctgcaaatgg tttgtctacg gataccattc cttactatac atctgcttac
     2161 gatatggaca tgcgcaaagt tattgatgtc tatgccgctg cgaccgaaca tgtagaccaa
     2221 ggcttgtcat taactctatt ccttcgtagt gagttgccta tggagcttta tgagtggaaa
     2281 acacaaagca aacaaaccac tcgtgattta tccatcttac gaaactacgc tttcaataaa
     2341 ggcattaaat ctatctacta tatccgtacc tttacggatg atggggaaga agtgggcgca
     2401 aaccaatgtg aatcttgtgt catttaattg gttaagaagt aatgatgcta aagcatctat
     2461 tacttctacg gcagtcgctt taggcgatct gcctaaacca ctcactaact atgtaaggaa
     2521 agtattatcg actttcctta ctccgtgtcg taattcctcg atatgcccta atgatgctaa
     2581 agcatctatt acttctacgg cagtcgcttt aggcgatctg cctaaaccac tcactaactc
     2641 attcgttacc cttttgaatc tatttaaact tattattacc actagaactt tggtcggttc
     2701 tactggttta tctcgatatc cttaacgcac tttactactc tttaggagat catttttcta
     2761 tgactacata ttatgaagca attaactgga atgaaatcga agacgtcatt gacaagtcaa
     2821 cttgggaaaa gttgaccgaa caattttggc ttgatacccg tatcccttta tcaaatgact
     2881 tagacgattg gcgcaaactt tctcttcaag aaaaagatct tgttggcaag gtttttggag
     2941 gattaaccct acttgatacc atgcaatcag aaactggtgt cgaagctatt cgtgctgatg
     3001 ttcgcactcc tcacgaagaa gctgtcttaa acaatattca attcatggaa tctgttcacg
     3061 ctaaatctta ttcgtctatc ttttcaacct taaacaccaa aaaagaaatt gaagagatct
     3121 ttgagtggac taataataat gagtttcttc aagaaaaagc acgtattatc aatgacattt
     3181 acgctaatgg agatgcctta caaaagaaag ttgcctcaac atatctcgaa actttccttt
     3241 tttattctgg ctttttcacc cctctttact atttgggaaa taataagcta gcaaatgttg
     3301 ctgaaatcat taaactaatt attcgtgatg aatctgtaca tggtacttat atcggttaca
     3361 aattccagct tggttttaac gaactaccag aagatgagca agagaatttt cgtgattgga
     3421 tgtatgacct cctttatcag ctgtatgaaa atgaagaaaa atacaccaaa acgctttatg
     3481 atggcgtagg gtggactgag gaagttatga cctttttacg ctacaatgct aacaaagctc
     3541 ttatgaattt aggacaagat cctttattcc cagatactgc aaacgatgtt aacccaattg
     3601 ttatgaatgg tatttcaaca ggaacatcaa accatgactt tttctcacag gtagggaatg
     3661 gctatcttct tggttctgta gaagctatgt ctgatgacga ttataattat ggactctaat
     3721 agtgctcacg agactgataa tctatcagtc tcttttctta acttttgtta taatagtctt
     3781 atgaaacgac actttttact cttgaccttt tacctctttt taacaggttt aacagctggc
     3841 cttgttgctt ttatcttaac aaaagccatt cacctgattc agtccctcag ttttggattc
     3901 agtcaaggtt cttttagcac tatgattgct agcgttccac cacaaagacg agctctttca
     3961 cttctctttg ctggacttct tgcaggtctt gggtggcacc tattggctaa aaaaggaaag
     4021 gacattcaat ctattcagca aatcattcaa gatgacatct catttagtcc ctggacacag
     4081 ttttggcatg gttggctcca attaacgacc gtatctatgg gagctcctgt cggccgagaa
     4141 ggagcttcac gggaagtcgc tgttaccttg acgtctcttt ggagccaaag atgcaacttg
     4201 tcaaaagccg atcaaaaact tttattagct tgcgcttcta gcgctgccct tggtgctgtc
     4261 tacaatgctc ccttagccac tattttattt atcttggaag ctattcttaa ccgttggtcc
     4321 cttaaaaata tatacgctgc ctgcctaacg agctatgtgg ctgtggaaac agttgcttta
     4381 ttacaaggcc gacatgagat tcaataccta atgcctcagc aacattggac gctaggaacc
     4441 cttatttggt ccgtcctggc tggtcttatc ctttccctct ttgctcacgc ttacaaacat
     4501 cttttggaac accttcctaa ggctgatgcc aaaagtcagt ggtttattcc gaaagttctc
     4561 atcgctttta gccttatcgc aggactcagc attttcttcc cagagatttt aggaaatggc
     4621 aaagctggat tactcttttt tctccatgaa gaaccacatc tgagctatat tagctggcta
     4681 cttgttgcca aagctgtcgc tatctcttta gtttttgctt ctggagcaaa agggggtaaa
     4741 attgctcctt ctatgatgct tggaggtgct agtggcttac tactagctat ccttagtcag
     4801 taccttattc ccttgtctct atcaaatacg ttagccatta tggttggtgc aacaatcttt
     4861 ttaggtgtga tcaataagat acccttggcg gcaccagttt ttctggttga aattacaggc
     4921 caatcgttac taatgattat ccctttagca ttagctaacc tcatttttta ttttagctat
     4981 cagttttatc gtttcatcct taaatgatta gttaattact tttttaataa aaagcttcgg
     5041 atgaattgga cttggtatac taaagtagac acaaaattaa gtggaaacga ggaa
//
LOCUS       DATURV010000077         5066 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DATURV010000077 DATURV010000000
VERSION     DATURV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.077"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            72..1733
                     /locus_tag="VCF75_001479"
     CDS             72..1733
                     /locus_tag="VCF75_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP2547030.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKDKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            1866..2651
                     /locus_tag="VCF75_001480"
     CDS             1866..2651
                     /locus_tag="VCF75_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEP2547031.1"
                     /translation="MANIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPKTLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFAANEKAIADF
                     RAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            2681..3007
                     /locus_tag="VCF75_001481"
     CDS             2681..3007
                     /locus_tag="VCF75_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547032.1"
                     /translation="MKQLLAIILWLPKLIVKLFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            3053..4960
                     /locus_tag="VCF75_001482"
     CDS             3053..4960
                     /locus_tag="VCF75_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP2547033.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDQTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQEALME
                     EWETIAEQIES"
BASE COUNT         1597 a          857 c         1123 g         1489 t
ORIGIN      
        1 tagctacata acagtaattc gacttacgtt gtaaggaccg tttgtaataa taaaatattt
       61 aaaggagtat catgactgat attaaaatga ttgccttggg aggggtccgt gaatacggaa
      121 aaaacttcta tttggtagaa attaacgatt ctatgtttat tttagacgca gggctcaagt
      181 accccgaaaa tgagcaactt ggggtagatt tagtcatccc caatttagat tatgtaattg
      241 aaaacaaaga taaggtacaa gggattttct tgagtcatgg ccatgctgac gccattggag
      301 ctcttcctta tttgttagct gaagtatcag ctcctgtatt tggatctgaa ttgactattg
      361 aattagccaa gctatttgtg aaaagtaata acagcactaa gaaatttaat aattttcatg
      421 tggtagacag tgatactgaa attgaattta aagatggcct agtttcgttc tttagaacaa
      481 cgcattcaat cccagaaagt atgggtattg ttattggaac agataaagga aacattgttt
      541 ataccggtga cttcaaattt gatcaagcag caagagaagg ttatcagacg gatttgctac
      601 gtttagctga gattgggaaa gaaggggttc tagctctttt atcagattca gtcaatgcga
      661 caagcaacga tcagattgct agcgaatctg aagttggtga agaaatggac agtgttatct
      721 ctgatgctga tggccgtgtc attgtagctg cagtagcctc aaatcttgtt cggattcaac
      781 aagtatttga ttccgccact gcccatgggc gtcgtgttgt tttaaccgga acagatgcag
      841 aaaacattgt cagaacagct ttacgtctag aaaaattaat gattacggat gagcgtcttt
      901 taattaaacc taaggatatg tctaaattcg aagaccatga attaatcatc cttgaagctg
      961 gtcgtatggg tgagccaatt aatagcttac aaaaaatggc agcgggacgt caccgttatg
     1021 ttcaaatcaa agaaggagac cttgtttata ttgtaacaac accaagtacg gctaaagaag
     1081 ctatggtggc gcgtgttgaa aacttgattt acaaggccgg tggttctgtt aaattaatca
     1141 ctcaaaactt acgcgtttca ggccatgcta atgggcgtga tttgcaacta ttgatgaatt
     1201 tgttaaaacc ccaatatctt tttccagtac aaggagaata tcgtgattta gctgcccatg
     1261 caaagctagc tgaagaagtt ggtatttttc cagagaatat tcatattctg aagcgtggcg
     1321 atatcatggt gcttaatgac gaaggttttc tccatgaagg aggtgtacct gccagtgatg
     1381 tcatgattga cggtaatgct atcggtgatg tgggtaatat cgttttacga gaccgtaaag
     1441 tcttgtcaga agatggtatt ttcattgttg ctattactgt ttctaaaaaa gaaaaacgca
     1501 ttatttctaa ggctaaagtt aatacgcgtg ggtttgtgta tgtcaagaaa agtcatgata
     1561 ttttaagaga aagtgcagaa ttagttaata caactgttgg caattactta aaaaaagata
     1621 cctttgactg gggcgaatta aaagggaatg tcagagatga tttgtctaaa ttcttatttg
     1681 aacaaaccaa aaggcgccca gcgattctgc cggttgtaat ggaagttcgt tagtgaaaat
     1741 aggaaggtta ggcaaaaact gcctaccttc tttgatttta acaactggta gaccaggaaa
     1801 ctgttcaccc acaaatacag tagatttacg tgataggatt attttggtat aaaggagtgt
     1861 aatgaatggc aaatattgca attgaatacc attcggtagt tttaggaatg gagcgtaagg
     1921 taaatgtgat ttatcctgac cagtctgaaa tccctaaaaa agatcaaggg gataaggata
     1981 ttcctgtttt atatttgtta catggtatgg gaggcaatga aaactcttgg caaaaacgaa
     2041 cagctattga acgcttgttg cggcatacca atttgatagt cgttatgcct tcgacagacc
     2101 taggttggta tacagacacg gcttatggac ttaactatta tcgggcgttg tcacaagagt
     2161 taccgcaggt cttagcagct ttctttccta atatgaccca gaaacgagaa aaaacctttg
     2221 ttgctgggct ctctatgggt ggctatggtg cctttaagtg ggcactcaaa agcaatcggt
     2281 tttcttatgc ggcatcgttt tcaggcgctt tggatttttc tcctaagacc ttgcttgagg
     2341 gaaacctggg agaattagcc tattggcagg gcgtttttgg ccagtttgat gaccctgacc
     2401 ttgataagca ttaccttaaa aatatggtgg cagaaagtga tggaaaaacc aaattttatg
     2461 cttggtgtgg ttatgaggat ttcctttttg ccgctaatga aaaagctatt gctgattttc
     2521 gagctcaagg tcttgacatt gactatcata aaggtcatgg taaacatgag tggtactatt
     2581 ggaaccaaca attagaagtt ctgttagagt ggttaccgat aaactaccaa aaagaagaaa
     2641 ggttatctta accatttcct agtacaacat gaggtgacga atgaaacaat tactggcgat
     2701 cattttgtgg cttcctaagc taatagtgaa actgttttgg catcttatca aagggttttt
     2761 acaaactatc ttattagtaa cgattattat tatagggctg atgtattatt ctaatcacag
     2821 tgattccgtt ttagccaata agatctcgat agttactgag caagtggtgc aaatttttga
     2881 tatcttaaca caaaagccat ctgcaaagac gcgtcatggg agtggtaata gtcattccac
     2941 tttccttgtt agcgctaaag aaggtaatca acgacttaaa aagagttcga tttcttttgg
     3001 aaactaggct gaaaatgtcc tcaaaagggc atttttttgt tagaatgaaa gtatgattat
     3061 tttacaagga aacaagttag aacgctcttt ctcaggagat gttctttttc aaaatatatc
     3121 actgcaagtt gatgaaagag acagaattgc attagtaggg ccaaatggag ctggtaagtc
     3181 aaccttactc aagctattgg ttggggaaga aaccccaacg agcggagaag ttaatactaa
     3241 aaaagaccta accttgtctt atctggctca aaatagccgt tttgaatcag atcaaacaat
     3301 atatgaggaa atgctcaagg ttttcgaagc attacgccaa gatgaaaagc gcctgcgtca
     3361 gatggaaatg gatatggcaa ccgtttcagg tcaagattta acccgactga tgacagatta
     3421 tgatctgctt acagagcatt ttcgtcagca aggcggcttt acgtatgaat ctgacatcaa
     3481 agccatttta aatgggttta agtttgatga gagcatgtgg cagatgacta tcgctgagct
     3541 gtcagggggt caaaatactc gtctggcctt ggctaaaatg cttttagaaa agccagaact
     3601 attggtcctt gatgagccaa caaaccactt agatattgaa accattgcct ggttagagaa
     3661 ctacttggcc aattatcaag gagctctaat cattgtgagt cacgaccgtt attttttgga
     3721 taaggtggca acagtgacct tggatttaac tcctcatgga ttggatcgct acgttggcaa
     3781 ttattctcgt tttatgacct taaaagctga aaaattagta gccgaagaaa agcagtttga
     3841 caaacagcaa aaagaaattg ccaagttaga agattttgtt caaaaaaata ttgtcagggc
     3901 ttcaactaca aaacgtgctc aggcgagacg taagcaattg gaaaaaatag aacgcttaga
     3961 caaaccgact agtgctagaa aatcagctca catgactttt caggcagata agccgtctgg
     4021 caatgtggtg ttgacggttg aaaaagctgc tattggttat aaccaacatg tcttatctga
     4081 acctattaac ctagacgttc acaaattaga tgcgattgct attgttggtc caaatggtat
     4141 tggtaaatcc actctgatta aatctgttat tggacagatt cctttcatca aaggagaagt
     4201 gaaatacggt gccaatgttg aaataggtta ctatgaccaa acccaatcac atttgacgtc
     4261 tagcaacaca gttttggaag aattatggca ggatttttca actacgccag aagtcgatat
     4321 cagaaaccga ttaggggctt ttctcttttc tggagatgat gtcaaaaaat cggtggctat
     4381 gctgtcaggt ggagaaaaag cccgtctgct tcttgcgaag ttatctatgg aaaacaataa
     4441 ttttcttgtt ctggacgagc cgactaacca cctagacatt gacagtaaag aagtgctaga
     4501 gaatgccctc attgactttg acggtacctt gctttttgtc agccacgacc gctatttcat
     4561 taatcgtttg gcaactaagg ttttggaaat taccgaaaat ggctcaaccc tttacttagg
     4621 tgactatgat tattaccttg agaaaaaggc agagctagaa gaattagccc gcctagcagc
     4681 aggagagaca gttgaggaaa caaaagaagc ctctgccact gattatcagc tacaaaaagc
     4741 taatcaaaaa gagcgtcgtc gcttgacccg ccgatatgag gagattgaag caagacttga
     4801 aaccattgaa gagcgtattg gtgctattca agaagacatg catgcctcca atgatacggc
     4861 gcagttaatc gcttggcaaa aggaatggga ccaattggat caagaacaag aagctcttat
     4921 ggaagaatgg gaaaccatcg ctgaacaaat agaatcataa aaataagctt tagcagtttt
     4981 gctaagctta tttttatcgc tttgctactt gtttgaaggt gtgaaagagg ttttaaaatg
     5041 atgtgttttt tatgtcaagg tcggta
//
LOCUS       DATURV010000078         5065 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DATURV010000078 DATURV010000000
VERSION     DATURV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.078"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            26..211
                     /locus_tag="VCF75_001483"
     CDS             26..211
                     /locus_tag="VCF75_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2547034.1"
                     /translation="MWEKIEQLMIERNLNMNKLAKLTGIHKSHFSDLKSGRIKHLSWP
                     NMVKLSTGLKVSLDEFR"
     gene            285..515
                     /locus_tag="VCF75_001484"
     CDS             285..515
                     /locus_tag="VCF75_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547035.1"
                     /translation="MLAKLKSGIEVPYEELWMNDNDLAEFIGKSFDQTQRLLRKMYKD
                     RNYRKYIDKVGGRSTKVKKFEEWRKTQNEKLI"
     gene            499..639
                     /locus_tag="VCF75_001485"
     CDS             499..639
                     /locus_tag="VCF75_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547036.1"
                     /translation="MKSLFNFIFAKPKKEEKPKWTIETHGWEANARRYNQLHGLPGKQ
                     VW"
     gene            649..867
                     /locus_tag="VCF75_001486"
     CDS             649..867
                     /locus_tag="VCF75_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547037.1"
                     /translation="MEQHLINDNEFLRDENRRLNNELAEHYFVVTAKANLLDVIIASG
                     FILQSTIDKCIAELDEIDQMELRKVWNK"
     gene            869..1111
                     /locus_tag="VCF75_001487"
     CDS             869..1111
                     /locus_tag="VCF75_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547038.1"
                     /translation="MRLAKQKIGAQQNGYGRIGCDIEIYNEIAEIADKCGYTLTSLTN
                     ALLGYALENSELTAETKTVEVFKFTIGNEDFDNGNN"
     gene            1095..2414
                     /locus_tag="VCF75_001488"
     CDS             1095..2414
                     /locus_tag="VCF75_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2547039.1"
                     /translation="MMVTINKLEIENVKRIKAVKIEPSATGLTIVGGNNNQGKTSVLD
                     AIAWALGGNKYKPSQATREGSMVPPTLKITMSNGLTVERKGKNASLKVVDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDGTPKEKADVLLEIIGVGDQLAELEFKEKEIYNQRHAIGV
                     IADQKEKFAKEMTYYPDAPKQLVSISELIQQQQAILAKNGENAQKRQNVERIRYDYDQ
                     SILEVDRLRKLLADAEAKTNKLSEDLKIANTDAMDLHDESTAEIEANIADIDEVNRKV
                     RANFDKDKAEEDAKQQRDQYNTLTNEIEVIRQQKRDLLTNADLPLEGLTVDDGKLLYL
                     GQEWDNMSGSQQLMVATAIVRKLKPECDFVLIDKLEQMDNITLTQFGQWLEQEGLQAI
                     ATRVSTGEECAIIIEDGYSVENKAHNFETAAAGSFAETVAPAWQGGF"
     gene            2429..3511
                     /locus_tag="VCF75_001489"
     CDS             2429..3511
                     /locus_tag="VCF75_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2547040.1"
                     /translation="MQITRGKRARAQRVVIYGPEGIGKSSFAAQFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAWIKANPTCCKTLVLDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNSLQELIEFGINVVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTTDTNKKKATGGSRVLYTQHHPAWDAK
                     NRHGLPEEMPLDYAGIAHIFAQQAPSQPVAETPSTQQPTPTQSAHEPAHKQPTQPPVA
                     NESQAPLPDTLPQAMKDLMVPDQVTADELVQVANIRGHFPLGTPIENFPPNYWDMIVA
                     NWSATLEVIKTQVRVEPDLPFTVEGT"
     gene            3706..4299
                     /locus_tag="VCF75_001490"
     CDS             3706..4299
                     /locus_tag="VCF75_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547041.1"
                     /translation="MTEYNNNFDHELGWDDEITQDSVGFVQLVPGDYQFTVTNMERGR
                     HTPDPQNPGKLPACNKAVVTIQIETSEGIAQLTHNLFLYTTTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVVGAKGVCRVNKRKGTGKYADQEYDNIKSMIYADEVDWSKVLNASQPT
                     QNTYQQPQQPTQQQPQPQQGSFFQGNTTQPQGGWGGF"
     gene            4299..>5065
                     /locus_tag="VCF75_001491"
     CDS             4299..>5065
                     /locus_tag="VCF75_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP2547042.1"
                     /translation="MQLRPYQEEARSAVQKEWEEGRKRTLLVLPTGCGKTIVFSKIIE
                     DRVRLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAENTSLGSWFRVVVGSVQTM
                     QREKRLSKFPANHFDTIIIDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLSLDLTTVSQQAGDFKASDIGTALDPYL
                     EQITDEMVKQCANRKTVVFLPLVKTSQKFRDILNQKG"
BASE COUNT         1780 a          905 c         1104 g         1276 t
ORIGIN      
        1 acatgttgac ttttcaatat agattatgtg ggaaaaaatc gaacaattga tgattgagag
       61 gaatctcaat atgaacaaat tagcaaaatt gacagggatt cacaaaagcc actttagcga
      121 tttgaaaagt ggaagaataa aacatctgtc atggccgaac atggtcaagc tatctacagg
      181 attaaaagtc agcttagacg aatttagata acaaaaaagt ccgacggcaa tcggactcaa
      241 atcaaatttt aatttactta gattatacca tgaaaggaaa cgatatgcta gcgaaattaa
      301 aaagcggtat tgaagtacct tatgaagaac tatggatgaa tgataatgat ttagctgaat
      361 ttattggaaa gtcatttgat caaacgcagc gattactaag aaagatgtac aaagacagaa
      421 attaccgtaa gtacattgac aaggttggtg gtcgttcgac aaaagttaaa aaatttgaag
      481 aatggagaaa aacacaaaat gaaaagctta tttaacttta ttttcgcaaa acctaaaaaa
      541 gaagaaaaac caaaatggac aattgagaca cacggctggg aagctaatgc acgtagatat
      601 aaccagttgc atggcttacc tggtaagcag gtttggtagg agggaagaat ggaacaacac
      661 ctaattaatg ataacgaatt tttgcgagac gaaaatcgca ggttaaataa tgaattggcg
      721 gaacattatt ttgtggttac agctaaagcc aatttattag atgtcattat tgccagtggt
      781 tttatcctgc aaagtacgat agataaatgt atagccgaat tggacgaaat tgatcaaatg
      841 gaacttagaa aggtatggaa taaatagtat gagattagca aagcaaaaaa ttggagctca
      901 acaaaatgga tatggtcgca tcggatgtga tattgaaatt tataatgaaa ttgctgaaat
      961 tgcagataaa tgtggatata ccctaacgag cttaacaaat gctcttttag gttatgcttt
     1021 agagaatagc gagctcacag cagaaacaaa aacagttgaa gtttttaaat ttactatagg
     1081 aaacgaggat ttcgataatg gtaacaatta ataaattgga aattgaaaac gttaagcgta
     1141 ttaaagcggt aaagattgag ccgtcggcaa ctggcttgac tattgttggt ggaaacaata
     1201 accaagggaa aacaagcgtt ctagacgcaa ttgcttgggc actaggcggc aataaataca
     1261 aacctagcca agcaacgcga gaagggtcta tggtgccgcc tacgcttaaa atcaccatgt
     1321 ctaatggact tactgttgaa cggaaaggta aaaatgccag tcttaaagtc gttgatccaa
     1381 acggccaaaa aggcggacaa caattactag atagctttgt tgaagagtta gctattaact
     1441 taccaaagtt tatggatgga acaccaaaag agaaagcaga tgtcttgctt gaaattattg
     1501 gtgtcggtga tcaattagct gaattagaat tcaaagaaaa agaaatctat aatcaacgtc
     1561 atgctatcgg tgttatagcg gaccaaaaag aaaagtttgc taaggagatg acatattatc
     1621 ctgacgcacc taaacaactg gtaagtattt ctgagcttat ccaacaacaa caagctatct
     1681 tagccaagaa cggagagaat gctcaaaagc gtcaaaatgt agaacgtatc cgctatgatt
     1741 atgatcaatc tatcttggaa gttgaccgct tacgcaagtt attagcagat gcagaagcta
     1801 agacgaataa gttaagcgag gatttaaaaa tagcaaatac tgacgctatg gatttacatg
     1861 atgaatcaac agcagaaatc gaggcaaata tcgccgatat cgacgaagtc aaccgtaaag
     1921 ttcgcgctaa ctttgataaa gataaggcag aggaagacgc taagcagcaa cgtgatcagt
     1981 acaacacatt gactaatgag attgaggtta ttcgccagca gaaacgagat ttattgacta
     2041 atgcagactt gccacttgag gggcttaccg ttgatgatgg caaactgctg tatcttggcc
     2101 aggagtggga caacatgtca ggaagtcaac aacttatggt agccactgcc attgtacgaa
     2161 aacttaaacc agagtgtgac ttcgttttga ttgataaact cgaacaaatg gataacatca
     2221 cacttacaca gtttggccaa tggctagagc aagaaggatt gcaagctatc gcaaccagag
     2281 tttcaaccgg tgaagagtgc gcgataatta tcgaggacgg ctactccgtg gaaaataagg
     2341 ctcataattt tgaaactgca gcagcaggta gttttgccga aacagtagca cccgcttggc
     2401 aaggtggatt ttagaaagga gaatatcaat gcaaatcaca agaggaaaac gtgcacgggc
     2461 tcaacgtgtg gtcatctatg gtcctgaagg gattggtaag tctagctttg ctgctcaatt
     2521 cccagagcct ctgtttattg acactgaggg atcaacagac aatatggatg tggcccgtat
     2581 ggataaacca acaagctaca ctatgctaaa aaatcaaatt gcatggatta aagcgaatcc
     2641 aacttgttgc aagacacttg tccttgacac aattgactgg gcagaaagcc taatcgttga
     2701 tgatgtgtgt gctcaacacg gtaaaaaggg aattgaagat tttggctggg gtaatggtta
     2761 tacctacacc aaagaagagg ttggacgctt cctaaacagt ctgcaagaac tgattgaatt
     2821 tggtatcaat gtcgtcctta ctgcccatgc ccaaatgcgt aaatttgaac aacctgacga
     2881 gatgggagct tatgaccgtt gggaattgaa acttggtaaa aagacaagct cacagactgc
     2941 tccgctcgtc aaggaatggg ctgatatggt actctttgct aattataaaa ctgttgttat
     3001 gacaactgat actaacaaaa aaaaggctac tggcgggtct cgtgttttgt acacgcaaca
     3061 ccacccggcg tgggatgcta agaaccgtca cgggttacca gaagaaatgc cactcgatta
     3121 tgctggtatt gctcatatct ttgctcagca agcaccaagt cagccagtag cagaaacgcc
     3181 atcaacacaa caaccgacgc ctactcaatc cgcacacgaa cccgcacata agcaaccgac
     3241 acaaccgccg gtagcaaatg aatcgcaggc accactgccc gacacattac cacaagctat
     3301 gaaagatttg atggtacctg atcaagttac agcggatgaa ctagtacaag tggctaacat
     3361 acgcggacat ttcccgcttg ggacgccgat tgaaaacttc ccccccaatt attgggatat
     3421 gattgttgca aattggtcag caactcttga agttatcaaa acacaagtaa gagtcgaacc
     3481 agacttaccc tttaccgtgg aagggactta gattttggga aatagaaata atagcgaggt
     3541 ataacaaaaa tggataaaac aatcaaattt gacttatctg ccattggtga tggtggaaag
     3601 tggcgcttag atgctatcaa taatattgct aactacttaa aagaagaact tgctgatcaa
     3661 actaacatta caattttagc ttaatagaaa ataggagaaa taaaaatgac tgaatacaat
     3721 aacaacttcg accatgaatt gggatgggat gatgaaatta cacaagacag tgtaggtttt
     3781 gtccaattag tgcctggtga ttatcaattt acagtaacta atatggagcg tggtcgtcac
     3841 acacctgatc cgcagaatcc aggaaaatta ccagcgtgta acaaggcggt cgttacgatt
     3901 caaattgaaa catcagaagg aatcgcgcaa ttaactcata acttattcct atatactaca
     3961 acagaaggta tgctatcagc tttctttggt gctattggtc aaaagaaaca tggggaacca
     4021 cttaaaatga attggaatac tgtcgttggt gcaaaaggtg tctgtcgtgt aaataagcga
     4081 aaaggaactg gtaaatatgc tgatcaagaa tacgataaca tcaaatctat gatttatgca
     4141 gatgaagttg attggtcgaa agttttgaac gctagtcaac caacacaaaa tacttatcaa
     4201 caaccgcaac aaccaactca gcaacaacct caaccgcaac agggaagttt cttccaaggt
     4261 aatacaacac aaccacaagg tggatgggga ggattctgat gcaattaagg ccgtatcagg
     4321 aagaggcacg gtcagccgta caaaaggaat gggaggaagg tagaaaacga actctactag
     4381 tcctccctac cggttgcggt aaaactattg tcttttcgaa aattatcgaa gaccgtgtaa
     4441 ggctcggaga gagagttttg gttctagctc ataggtctga acttttagaa caggctagtg
     4501 ataaattgat gacagcaact ggattaggga cagcattaga aaaagcagag aatacttctt
     4561 tggggtcatg gttcagggtt gtagttggat cagttcaaac gatgcagaga gagaagcgtt
     4621 tgagtaagtt tcctgcaaat cattttgaca caattattat tgatgaagcc catcatgcca
     4681 tctctgatgg ttaccaaaga gttttgcaac attttgatag tagcaatgtt ttgggagtta
     4741 cagcgacacc agatcgtggt gacaaacaaa atctaggtaa atatttcgat agtttagctt
     4801 atgaatattc tttggtagat gccatcaaat ctggttatct atcaaaaatc actgctgtta
     4861 caattccact atcactggac ttaacgactg ttagtcagca agcaggagac tttaaagcta
     4921 gtgacatcgg aactgcctta gatccatatc ttgagcagat taccgatgaa atggtgaagc
     4981 aatgtgcaaa ccgtaaaaca gttgtatttc ttccgcttgt taaaacttcc caaaaattcc
     5041 gtgatattct aaaccaaaaa ggatt
//
LOCUS       DATURV010000079         5020 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DATURV010000079 DATURV010000000
VERSION     DATURV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.079"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            500..3526
                     /locus_tag="VCF75_001492"
     CDS             500..3526
                     /locus_tag="VCF75_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEP2547043.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQINSGSQLEQSKESLSLNKRVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINKAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            3546..3947
                     /locus_tag="VCF75_001493"
     CDS             3546..3947
                     /locus_tag="VCF75_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEP2547044.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAQGRMG
                     VPKAVGIIHIERIFNLQSGANAGKEINSESN"
     gene            complement(4127..5002)
                     /locus_tag="VCF75_001494"
     CDS             complement(4127..5002)
                     /locus_tag="VCF75_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEP2547045.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYRTLHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLRESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSSIEVN"
BASE COUNT         1778 a          786 c          952 g         1504 t
ORIGIN      
        1 ctgattaagt gaagctgtaa tgagacttat acgatcaaaa aagttagaat aactttataa
       61 aacagtggtg ttgaagcaag tttagcttct tctcagaaat agattatcta taaaaaatcc
      121 caaaagtagt ttgtttccag ttaatgaact tcttttggga aggctttttt gctggtcgcc
      181 acctcacttg tatatcggga accttcacgc taagatgtat aagttgaatc gtttgtctat
      241 ggcttgatga aaattaatca aaagataatg ttcaactcta taattggtac aagtaaaatg
      301 agataactta ttcattagta agcataagat ataacaagag aaattacctt aaaacaggaa
      361 gtactagagt atttaaaata agtgatagac ttggatatta ttaggtcgac tagtgtcctt
      421 ttcaataatt taatatcgtt attattagta tcagccaatc aaatgtggat aagttggctt
      481 ttagaaaggt tttaccacaa tgaagaaaca tcttaaaaca gttgccttga ccctcactac
      541 agtatcggta gtcacccaca atcaggaagt ttttagttta gtcaaagagc cgattcttaa
      601 acaaactcaa gcttcttcat cgatttctgg cgctgactac gcagaaagta gcggtaaaag
      661 caagttaaag attaatgaaa cttctggccc tgttgatgat acagttactg acttattttc
      721 ggataaacgt actactcctg aaaaaataaa agataatctt gctaaaggtc cgagagaaca
      781 agagttaaag gcagtaacag agaatacaga atcagaaaag cagatcaatt ctggatctca
      841 actagaacaa tcaaaagagt ctctttcttt aaataaaaga gtgccatcaa cgtctaattg
      901 ggagatttgt gattttatta ctaaggggaa tacccttgtt ggtctttcaa aatcaggtgt
      961 tgaaaagtta tctcaaactg atcatctcgt attgcctagt caagcagcag atggaactca
     1021 attgatacaa gtagctagct ttgcttttac tccagataaa aagacggcaa ttgcagaata
     1081 taccagtagg gctggagaaa atggggaaat aagccaacta gatgtggatg gaaaagaaat
     1141 tattaacgaa ggtgaggttt ttaattctta tctactaaag aaggtaacaa tcccaactgg
     1201 ttataaacat attggtcaag atgcttttgt ggacaataag aatattgctg aggttaatct
     1261 tcctgaaagc ctcgagacta tttctgacta tgcttttgct cacctagctt tgaaacagat
     1321 cgatttgcca gataatttaa aagcgattgg agaattagct ttttttgata atcaaattac
     1381 aggtaaactt tctttgccac gtcagttaat gcgattagca gaacgtgctt ttaaatcaaa
     1441 ccatatcaaa acaattgagt ttagaggaaa tagtctaaaa gtgatagggg aagctagttt
     1501 tcaagataat gatctgagtc aactaatgct acctgacggt cttgaaaaaa tagaatcaga
     1561 agcttttaca ggaaatccag gagatgatca ctacaataac cgtgttgttt tgtggacaaa
     1621 atctggaaaa aatccttatg gtcttgctac tgaaaatacc tatgttaatc ctgataagtc
     1681 actatggcag gaaagtcctg agattgatta tactaaatgg ttagaggaag attttaccta
     1741 tcaaaaaaat agtgttacag gtttttcaaa taaaggctta caaaaagtaa aacgtaataa
     1801 aaacttagaa attccaaaac agcacaatgg tgttactatt actgaaattg gtgataatgc
     1861 ttttcgcaat gttgattttc aaaataaaac tttacgtaaa tatgatttgg aagaagtaaa
     1921 gcttccctca actattcgga aaataggtgc ttttgctttt caatctaata acttgaaatc
     1981 ttttgaagca agtgacgatt tagaagagat taaagaggga gcctttatga ataatcgtat
     2041 tgaaaccttg gaattaaaag ataaattagt tactattggt gatgcggctt tccatattaa
     2101 tcatatttat gccattgttc ttccagaatc tgtacaagaa atagggcgtt cagcatttcg
     2161 gcaaaatggt gcaaataatc ttatttttat gggaagtaag gttaagacct taggcgagat
     2221 ggcattttta tcaaatagac ttgaacatct ggatctttct gagcaaaaac agttaacaga
     2281 gattcctgtt caagcctttt cagataatgc cttgaaagaa gtattattac cagcatcact
     2341 gaaaacgatt cgagaagaag ccttcaaaaa gaatcattta aaacaactgg aagtggcatc
     2401 tgccttgtcc catattgctt ttaatgcttt agatgataat gatggtgatg aacaatttga
     2461 caataaagtg gttgttaaaa cgcatcataa ttcctacgca ctagcagatg gtgagcattt
     2521 tatcgttgat ccagataagt tatcttctac aatggtagac ctcgaaaaga ttttaaaact
     2581 aatcgaaggt ttagattatt ctacattacg tcagactact caaactcagt ttagagacat
     2641 gactactgca ggtaaagcgt tattgtcaaa atctaacctc cgacaaggag aaaaacaaaa
     2701 attccttcaa gaagcacaat ttttccttgg ccgcgttgat ttggataaag ccatagctaa
     2761 agctgagaag gctttagtga ccaagaaggc aacaaagaat ggtcagttgc ttgaaagaag
     2821 tattaacaaa gcggtattag cttataataa tagcgctatt aaaaaagcta atgttaagcg
     2881 cttggaaaaa gagttagact tgctaacagg attagttgag ggaaaaggac cattagcgca
     2941 agctacaatg gtacaaggag tttatttatt aaagacgcct ttgccattgc cagagtatta
     3001 tatcggattg aacgtttatt ttgacaagtc tggaaaattg atttatgcac ttgatatgag
     3061 tgatactatt ggcgagggac aaaaagacgc ttatggtaat cctatattaa atgttgacga
     3121 ggataatgaa ggttatcatg ccttggcagt tgccacttta gctgattatg aggggctcga
     3181 catcaaaaca attttaaata gtaagcttag tcaattaaca tctattcgtc aggtaccgac
     3241 tgcagcctat catagagccg gtattttcca agctatccaa aatgcagcgg cagaagcaga
     3301 gcagttattg cctaaaccag gtacgcactc tgagaaatca agctcaagtg aatctgctaa
     3361 ctctaaagat agaggattgc aatcaaaccc aaaaacgaat agaggacgac actctgcaat
     3421 attgcctagg acagggtcaa aaggcagctt tgtctatgga atcttaggtt acactagcgt
     3481 tgctttactg tcactaataa ctgctataaa aaagaaaaaa tattaaattg atttaaggag
     3541 aagaaatgat aacacaagaa atgaaagatc ttattaataa ccaattagca atggttgcga
     3601 cggttgatgc caaaggtcaa cccaatattg gtccaaaacg ttctatgcgt ctctgggatg
     3661 ataaaacatt tatttataat gaaaatacag atggtcaaac tcgtattaac attgaagata
     3721 atgggaaaat tgaaattgcc tttgttgatc gtgagcgcct tttaggttac aggtttgtag
     3781 gaactgctga aattcaaaca gaaggtactt attacgaagc agcgaaaaaa tgggcacaag
     3841 gacgcatggg ggtaccaaaa gcagttggta ttattcatat cgagcgtatt tttaatttac
     3901 aatctggtgc taatgcaggt aaagaaataa acagtgaaag taactaaaat taactgtaac
     3961 agtatttagg acccttattt taaacgctaa aaaacaagca tactcttttt tacagcatag
     4021 tgactatgtt gtaaaaaggg gtatgcttat taggtgctct taatatttct agataactgc
     4081 acctaataag ttttcaatag acttatagca cattaattga cttcaattag ttgacttcaa
     4141 tactagaaag agaagaacag tgatgttggt gttcgtttaa agaagtatcg atttccacag
     4201 tgactgagtt tatattgtac ctttgacaaa tcagtctaat agattttttg caatgttttt
     4261 cagtggtaga ttctctcaag cagcaatgta tagttgctct atgatcaata ccatccatcg
     4321 accaaacatt aagctgatta acagacacta tatgaggaag ttggctgagt tcatggtgca
     4381 aagtgcgata gtctatcgaa tcaggaacac catctaaaaa aatgttagca gttgctacta
     4441 actttggaag agctttagat aatataaaag aagcaatggc aattgataat aaaggatcaa
     4501 gaatgtacca aggtttccat tttaagatca gtgacaatat gataatggct aaccacccaa
     4561 ggatatcttc taaaaagtga aggcttaata tttcttcatt ttttgtctgg ttagagtgaa
     4621 tgatgaagct agcaaatcca ttgattataa ttgcaaaaat agctaaaacg aacataccgt
     4681 cataattaac aatagtagga tgccataatt ttggtatcgt ttcaatcatt actaataatg
     4741 acccgctaat aagtattaga tttgttgtta gtgctcctaa aaggctaaat cgcttatagc
     4801 ctagtgaaaa atttggactc tcttttttaa cagcctttct ttctaagata gctgagatac
     4861 caatagcaat ggcatctcca aaatcgtgga cagcatctgc taaaatagca cccgaaaaga
     4921 ataatgtccc aaaaataaat tcaatgaggg aaaaactaag atttaatata aaaataatgg
     4981 tgactttttt acttgctggc ataattttct ccttttgtta
//
LOCUS       DATURV010000080         4890 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DATURV010000080 DATURV010000000
VERSION     DATURV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.080"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..1033)
                     /locus_tag="VCF75_001495"
     CDS             complement(<1..1033)
                     /locus_tag="VCF75_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEP2547046.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTDAIVQLVKTTICGTDLHI
                     LGGDVPACKEGTILGHEGIGIVKEVGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSS
                     HCEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVL
                     PSHVNPGDNVCIVGAGPVGLAALLTVQFFSPANIIMVDLSQNRLEAAKAFGATHTICS
                     GSSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFN
                     LDELWIKNITLNTGLVNANTTEMLLNVLKTGKIDATRLITHHFKLSEVEKAYETFKHA
                     GANNALKVII"
     gene            1269..2000
                     /gene="aphA"
                     /locus_tag="VCF75_001496"
     CDS             1269..2000
                     /gene="aphA"
                     /locus_tag="VCF75_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEP2547047.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINATKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            2178..3710
                     /locus_tag="VCF75_001497"
     CDS             2178..3710
                     /locus_tag="VCF75_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEP2547048.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVVVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            3873..4487
                     /locus_tag="VCF75_001498"
     CDS             3873..4487
                     /locus_tag="VCF75_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEP2547049.1"
                     /translation="MLEIVSEELRRYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQAYI"
     gene            4646..>4890
                     /locus_tag="VCF75_001499"
     CDS             4646..>4890
                     /locus_tag="VCF75_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP2547050.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVL"
BASE COUNT         1514 a          793 c          942 g         1641 t
ORIGIN      
        1 caataataac tttcaaggcg ttatttgcac cagcatgttt aaaggtttca taagcttttt
       61 caacttctga aagtttaaaa tgatgtgtaa taagtcttgt tgcgtcaatc tttccagttt
      121 tcaagacatt tagtagcatt tcagttgtat tggcattaac aagtccagtg tttaaggtga
      181 tatttttaat ccataattca tccaggttga aatcaactgg tttcccatga accccaacgt
      241 tagcaatatg cccacctact gatataattt tttggcaaat atcaaatgtc gcgggataac
      301 caacacactc catggaaata tcgacaccac gtccattggt tatatcgtca atgatagcct
      361 tgacttcttc actacttcct gaacaaatcg tgtgtgtggc tccaaacgct ttagctgctt
      421 caaggcgatt ttgagataag tcgaccataa taatattggc tggagagaaa aattgaacgg
      481 ttagtagggc tgcgagtcct actggaccgg caccaacaat acaaacatta tctccaggat
      541 tgacatgtga tggcaagaca ccaatttcat atgaagttgg taaaatatca gaaagcatga
      601 caagtgcctc atcatcaatc gtatcaggag catgatagag actaccatct gcatgtggga
      661 tatggacata ttccgcttgt gttccattaa ttaaatggcc aagaatccaa ccgccatctt
      721 cacaatgtga cgaaaggcct cgtttacaat aataacaggt atgacatgat gtgacgcaag
      781 aaataatcac tttatctccg attttaaagt tagtaaccgc atcaccgacc tccttaacaa
      841 ttccaattcc ttcatgacca aggattgttc cttctttaca tgcgggaaca tccccaccta
      901 agatatgcaa atcagttcca caaatagtgg tttttaccaa ctggacgata gcatctgtag
      961 gctttataat gactggtttt ggtttgtcta tgagctgtag atttccggtt gaaaggtaag
     1021 ttgctgcttt catatttaac tccttataag gttgagaggt aatcaaccaa ttcattttta
     1081 cacagatatt ataatagttt aagaaagggt tttcaatatt ttttaaatcg attatgataa
     1141 ttttttagat catctttgtt gaataacttc atccttattc agaacttaaa gggcactatt
     1201 ataagaattt gaaaccgctt tacttaaacc gtgtattttt gtacaataat gattaaggag
     1261 gtttttagat gaaaaaagaa tttaccagta tcctgtttac agttagtttt tgtggaatca
     1321 tagcattgcc tgtcgaagct agtggcccta aagttcctta tacacaagaa ggtataacgg
     1381 ccatttcaaa tcaggcaaca gtaaaattga tttctattgc tgatatcgcg agcagtttag
     1441 aaggacaaaa acctataacg gttagttttg atatcgatga tactttgtta tttactagcc
     1501 aatattttca gtacggcaaa gaatatataa ctccaggctc atttgatttt ttgcataagc
     1561 aaaaattttg ggacttagtc gctaaacggg gagatcagga ttctattcca aaagagtatg
     1621 caaagcaact aatcgcaatg catcaaaaac gaggagataa aattgtcttt attactggcc
     1681 gaactcgagg atcgatgtat aaaaaaggtg agattgataa aacagctaag tcactggcta
     1741 aagattttaa gttagacaag ccaatagcta tcaattatac aggagataaa gctgtgaagc
     1801 cgtatcagta tgataaaaca tattatataa agaaaaacgg ctcacaaatt cattacggag
     1861 atagtgatga agatataaat gctaccaaag aggcaggtgc aagacctatt cgcattttaa
     1921 gagctcctaa ttcaaccaat cttcctttgc caaaagctgg aggatacgga gaagaggttt
     1981 tagaaaattc ggcttattag tcaaatattt gtgtaagaaa ggaagtaaca gtaatttctt
     2041 tcatcacccc actgagtaac caatagccca gtgggttttt tgggttacag aaatcagttc
     2101 taaaaaagaa tcactggtaa cttaaggttg tctttgatac aatggtaaca gtgtatttaa
     2161 aagaaagaga ggacaacatg gaaaaccata aaaatgaatt caccttttcc aacaaatcaa
     2221 ttattgccta tgtttggcgt ggtgttgtgg ttggtattat tgctggtgtt attgttagtt
     2281 tattccggtt gttaattgaa gtgacagctg actgggtgat tgaatggtac cgatacgctc
     2341 atatcaattc gctgttgcta cttcctatac taagtgtcag tttgttagct gttctttttg
     2401 taggtttttt agtaaagtct gattctgata taaaaggatc agggatcccc catgtcgaag
     2461 gagaactaaa ggggctaatg tccccagatt ggtggagtgt cctttggaaa aaatttttag
     2521 gaggaatcat ggctatttct atgggattta tgctcggtcg tgagggtcct tcgattcagt
     2581 taggtgctat gtcagccaaa ggattagcta agtttttaaa atcgagtcgt ttggaaaagc
     2641 gagtgctaat tgccagcgga gcagcagcag ggctatcagc agcttttaat gctccaatag
     2701 caggcctctt gtttgttgtt gaagaaattt accatcattt ttcgcgttta atttggatta
     2761 cagcgctagt ggctagctta gtggctaatt ttatttctct gaatattttt ggattgaaac
     2821 cagtcttagc aatgtcagag gcgatgcctt ttttaggact caatcaatat tggttgttgc
     2881 ttttacttgg attatttcta ggctgcttag gctatttata tgagatagtt atccttaatt
     2941 ttaataaact ttatgttatt ttagggagtt ggttacatct gccagcttat ttttacggta
     3001 ttattatggt ttttctaatt ttaccgattg gctattattt accacagtta cttggaggag
     3061 gacatggttt aatactttct ttatccaatc aacaattgcc tttgatgact atcttctttt
     3121 attttattat tcgatttatt gttagtatgt ttagttatgg aagtggctta cctggaggta
     3181 tttttttacc cattttaact ttaggtgctt tggcaggcct attatttggg caaatagctt
     3241 ctcaattagg tttattgaac caatcttttc tttctctttt tttgatatta gggatggcag
     3301 ggtattttgc ggcaatttca aaagcaccac tgacaggcat gattttggta actgaaatgg
     3361 taggtgattt gaaaccctta atggccatag ctgtagttac ttttgtctcc tatttagtga
     3421 tggatttgtt aaatggacag ccaatttacg aagctatgct agataagatg gcaatgaagc
     3481 atccaactaa tcttgttgaa ccaacattaa ttgaattgac tgttggtgat aagattgcag
     3541 ggaaatatgt taaagagtta aagcttccag aaaatgtctt aattacaacc caaattcacc
     3601 atcaaaaatc tcaagttgtg tcaggtaaca cgcgcctttt atcaggagca actatctttt
     3661 tggtcgttaa tgaagctgat acaggttttg tccgtgaggt attgatgtag gtattaattt
     3721 atcacaattc ggaaagttcg agcttgtctt ttagactaag tctttgaacg ttttactgct
     3781 agagtttcat ctgaaccaaa gtgaaacgct tttgatgaag tcaagtaggc tttttgttat
     3841 actaacaata aagataagct aaggaggctg agatgttaga aattgtttca gaagagctac
     3901 gtcgctatca agaacaaaaa ttgattgagt accgtaacaa aaatcagtta gccccaaaag
     3961 gaggcattgt atttgcaggt gattctctca ttgaattttt tcctttaaaa aaagcttttg
     4021 gttcatgttt gcctattatt aatcgaggta ttgctgggat agatagtcag tggttattgc
     4081 gccatttttc tgtccaaatt actgatttag agcctaaaca tattttttta ttaattggtt
     4141 gtaatgatat cgggcttgga tatgataaat gtcatattgt caagactatt gtagagctta
     4201 ttagccagat tcgtagtcat tgtgtctaca gtcagattta tctcttatct ttattaccag
     4261 tttccaacaa tccccgatat cagaagactg tcaaaataag aacaaatgct atgattgatg
     4321 ctattaataa agatttagca atgattccaa ctatagaatt tattaatctt aatacttgtc
     4381 ttaaagacga aaaaggtggt ttatcagatg agaatactct agatggttta catcttaatt
     4441 ttccagctta tgctaaacta gctgaaatta ttcaagctta tatctagcaa ataaatgtac
     4501 taatagcgat gaggcaagaa gtcagatagc agatggttca tcaatcaatt aagaaaaaag
     4561 ttatcaagat accattaaaa atagttgctc aaagttcttt caaatcaaaa gattatgata
     4621 tcgctatgtt gagttttagt gtataatgat aattaaagga gttaccatga aatttgaaaa
     4681 aaaacaagtc ttctatttag tccttacgtt tatcttatgc tatgggatcc tagcaaactg
     4741 gcgaaatgga acggctatcg tcacgactat ttacaaaaca agccttcctt ttttttatgg
     4801 agcagcaggt gcctatattg ttaatattgt aatgagcgct tacgaaaagg tatatgttta
     4861 tatctttaaa gactggtcac atgtcttaaa
//
LOCUS       DATURV010000081         4798 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DATURV010000081 DATURV010000000
VERSION     DATURV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.081"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            60..1256
                     /locus_tag="VCF75_001500"
     CDS             60..1256
                     /locus_tag="VCF75_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP2547051.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPAAPSAGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYR
                     TGSTPMVGAIAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGV
                     TFIYPH"
     gene            1509..2471
                     /locus_tag="VCF75_001501"
     CDS             1509..2471
                     /locus_tag="VCF75_001501"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP2547052.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            2657..3412
                     /gene="recO"
                     /locus_tag="VCF75_001502"
     CDS             2657..3412
                     /gene="recO"
                     /locus_tag="VCF75_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEP2547053.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            3515..4522
                     /gene="plsX"
                     /locus_tag="VCF75_001503"
     CDS             3515..4522
                     /gene="plsX"
                     /locus_tag="VCF75_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEP2547054.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQMINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            4515..4757
                     /locus_tag="VCF75_001504"
     CDS             4515..4757
                     /locus_tag="VCF75_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP2547055.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHIAIPDEDVEDMVFMGDVLDYLVQRLT"
BASE COUNT         1554 a          738 c          992 g         1514 t
ORIGIN      
        1 atactttttt ttttggtaaa atataagatg tcttatcgaa aaggagtagt ttttttatta
       61 tgaaaaaaag aattttatca gcagttcttg taagtggtgt taccctcgga gcagctacaa
      121 ctgtaggagc ggaggattta agtactaaga ttgctaagca agattctatt atctcaaatc
      181 tgactacaga gcaaaaagct gcacagaatc aagtttcagc gttacaggct caagtaagtt
      241 cactacaatc tgaacaagat aaactgaccg caagaaatac agaacttgag gcgctttcaa
      301 agcgatttga gcaagaaatt aaggctctaa caagtcaaat tgttgctcgt aatgaaaaat
      361 taaaaaatca agctcgtagt gcttataaaa acaatgaaac ttctggttat attaatgcac
      421 ttttgaattc taaatcaatt tctgatgttg taaaccgttt agtagcaatt aatagagctg
      481 tctctgctaa cgctaaattg ttagaacaac aaaaagctga taaagtttcc cttgaagaaa
      541 agcaagctgc taaccaaaca gctattaata ccattgccgc taatatggca atggctgaag
      601 aaaaccaaaa tacattacgt actcaacaag ctaatttggt agctgcaact gcaaatttag
      661 ctctccaatt agcatctgct actgaagata aagctaattt ggtagctcaa aaagaagctg
      721 cagaaaaagc tgctgctgaa gccttagcac aagaacaggc tgctaaagtt aaggcacaag
      781 aacaggctgc acaacaagca gcatctgttg aagcagcaaa atctgctatt actccagcac
      841 cacaagctac tccggcagcg caaagtagta atgctattga accagctgca ctcacggctc
      901 cggcagctcc ttctgcagga ccacaaacat catatgattc ttctaatact tatccagttg
      961 gacaatgcac atggggagct aaatctttag ctccttgggc aggaaataat tggggaaatg
     1021 gtggtcaatg ggcttatagt gctcaagcag ctggttatcg tactggttca acgccgatgg
     1081 taggtgcgat tgccgtttgg aacgatggtg gttatggaca tgtcgccgtt gtagttgagg
     1141 ttcaaagtgc ctcaagtatt cgtgtgatgg agtctaacta cagtggtaga cagtacattg
     1201 ctgaccaccg tggctggttt aatccaacag gtgttacatt tatttatcca cactaacata
     1261 ttatttaggt tagaacagtt gttctaacct ttttgattac tcctattgtg gaatagttct
     1321 gagagtattg acgatatagt taatttataa gagagttgtt tggccaatcg cttatgtata
     1381 atataagaga agtatataaa gtcgataaaa ggtatattta atacattctt taacggtttt
     1441 tcaatttcat ttgataattc acttgaaaag cgttaaaatg gtataggatt aaaactttgg
     1501 aggaaaaaat gtcttattct gatttaaaat tgtttgcttt atcgtcaaac aaggaattag
     1561 cagaaaaagt tgcgtctgca atgggtattc aacttggaaa gtctacggtt cgccagtttt
     1621 cagatggtga aatccaagta aatattgaag aatcaattcg tggtcatcat gtctttattt
     1681 tgcaatcaac tagctcacct gtcaatgaca atttaatgga aattttgatt atggtcgatg
     1741 ctttaaagcg tgctagtgct gaaaaaattt cagttgtgat gccttactac ggctatgcac
     1801 gccaagaccg taaagcgcgt tctcgtgaac ctattacttc aaaattagtt gctaatatgt
     1861 tagaagttgc tggtgtagat cgcttattaa cggttgattt acatgctgct caaattcaag
     1921 ggttctttga tattccagtt gatcacttga tgggtgcacc tttaattgca gactattttg
     1981 atcgtcatgg tttagtgggt gaagatgttg tcgttgttag tcctgaccat ggtggtgtga
     2041 ctcgtgcgcg taaattagca caattcctcc aaacaccaat tgcaattatc gataagcgtc
     2101 gtagtgtgga taagatgaac accagtgaag tgatgaatat tatcggaaat gtttcgggca
     2161 agaaatgtat cttgattgac gatatgattg ataccgcagg gactatttgc catgcagcag
     2221 atgcgcttgc tgaagcgggg gcaacagctg tgtatgcgtc atgtacgcat cccgttcttt
     2281 ctggaccagc acttgataat attcaacgtt cggctatcga aaaactaatt gttcttgata
     2341 ctatttatct gccaaaagaa cgtcttattg ataaaattga acaaatttca attgctgatt
     2401 tggttgcaga agcgattatt cgtattcatg aaaaacgtcc gttgtcacct ttatttgaga
     2461 tgggcaatta aacttaacga agcctgagat aatagatctc gggctttttg acttgaataa
     2521 aatgtaaagg taatgtggta ttaattattg tgggatgaac tatgcaattc taaaggattt
     2581 tgggtataat aagaaagata ataacagttt attgtaaact aagataaaag gtaaaagaaa
     2641 aatacgacga ggtaatatgc aactaacaga atcactaggc attgttcttt ttaataggaa
     2701 ttatcgagaa gatgataaat tagtcaaaat atttactgaa gtagcaggta agcagatgtt
     2761 tttcgtgaaa catattagtc gttccaaaat gtcctcaatc attcaaccac taacgattgc
     2821 tgattttatt ttcaagttaa atgatacagg cttgtcttat gttgttgact atagtaacgt
     2881 taacacttat cggtatatta ataatgatat ttttcgatta gcctatgcta gttatgtctt
     2941 agcattagct gatgctgcga ttgcagataa tgaatcagat tcgcatttgt ttacgttttt
     3001 gaaaaaaaca cttgatttga tggaagaggg cctagattat gaaattttga caaatatttt
     3061 tgaaattcag atattagatc gttttggtat tagtctaaac tttcatgagt gtgccatttg
     3121 tcatcgcact gatttaccgc ttgatttttc ccatcgtttt tcagctgtac tttgttctga
     3181 acattattac aaagacaacc gacgtaatca tttagatcca aatgttatct acttgttgag
     3241 tcgatttcaa aaaatcacat ttgatgattt gagaactatt tcattgaata aagacatcaa
     3301 aaagaagctt cgtcagttca ttgatgagtt gtatcacgac tatgtaggaa tcaaattaaa
     3361 aagtaaaaca ttcattgata atttagttaa gtggggagat attatgaaat gatagggcct
     3421 ctttcgttta tgggaattgg tttcagaata tgctataata aaaatactat taatagattg
     3481 tgtaagaaca caagatataa ttaaaggagt ggaaatgaag agaattgcaa tagatgctat
     3541 gggtggagat aacgctccta aagctattgt tgagggtgtt aatcaagcaa tagaagcatt
     3601 ttcagatatc gaaattcaac tttacggtga tcagacaaag attaactcct atcttattca
     3661 atcagatcgt gttgctatta ttcatacaga tgaaaaaatt atgtcagatg atgaacctgc
     3721 aaaagctgtt cgccgtaaaa agaaggcttc aatggtttta gcagctaagg cagtgaaaga
     3781 aggaaaagca gatgccatta tttcagcagg taatacaggt gctcttttag ctgtaggttt
     3841 gtttgttgtg gggcgtatca agggagttga tcgtccagga ttattatcaa ctattcccac
     3901 agtaaccgga ttaggatttg atatgttgga tcttggagct aatgctgaaa atacggcaaa
     3961 acatttacat caatatgcta ttttaggctc tttctatgct aagaatgtac gtgggattgc
     4021 taatccacgt gttggtctat taaacaatgg tacagaagaa acaaaaggag atccccttcg
     4081 taaggctact tatgaacttt taacagcaga taatacaata tcttttgttg gtaatgtcga
     4141 ggcgcgagaa cttatgtcag gtgttgcaga tgttattgta tcagatggtt tcacgggaaa
     4201 cgcagtgttg aaatcaatag aaggaacagc tattagcatt atggggcaac tgaaacagat
     4261 gattaatagc ggcggtatta agactaaaat tggggctagt ctacttaaaa gtagtcttta
     4321 cgagatgaag aagactcttg actactctag tgctggaggt gcagttttat ttggtttaaa
     4381 ggcacctgtt gtaaagtctc atggttctag tgatgtaaaa gctatttttt caacgataaa
     4441 acaagtacgt acgatgctag acactaacgt ggtcggacaa ttagtagaag aatttgctaa
     4501 ggagactcaa gtaaatgact agacaggaga tttttgaaag gctgattaac ttaattcaga
     4561 agcaaagaag ctatttgagt gtagctatta cagaacaaac acatttaaaa aatgatttag
     4621 cggttgactc tattgaacta gttgaattta ttattaatgt tgaagatgaa tttcatattg
     4681 ctattcctga tgaagatgtt gaggatatgg tatttatggg agacgtttta gattatttag
     4741 ttcagcgttt gacttagtta gaataataaa aagagcgtaa gctcttttta ttattctt
//
LOCUS       DATURV010000082         4669 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DATURV010000082 DATURV010000000
VERSION     DATURV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.082"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(14..1282)
                     /locus_tag="VCF75_001505"
     CDS             complement(14..1282)
                     /locus_tag="VCF75_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEP2547056.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYYKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYSTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPYFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYRNFTLDKSDRIIIT
                     GKKRYLSIAINQQIQSKLNSLIKNTCGKSN"
     gene            1372..2238
                     /locus_tag="VCF75_001506"
     CDS             1372..2238
                     /locus_tag="VCF75_001506"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEP2547057.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLSLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLDYLTKSFKK
                     LLEEKNVISKIS"
     gene            2216..2773
                     /locus_tag="VCF75_001507"
     CDS             2216..2773
                     /locus_tag="VCF75_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2547058.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRIPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            2907..4451
                     /locus_tag="VCF75_001508"
     CDS             2907..4451
                     /locus_tag="VCF75_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP2547059.1"
                     /translation="MSILEVKQLSYGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
BASE COUNT         1507 a          694 c          910 g         1558 t
ORIGIN      
        1 gtacgaagaa aagttagttg ctctttccac aagtattttt gattagcgag tttaatttgc
       61 tttgtatttg ttgattgata gcaatagaga gatagcgctt ttttccagtt attattattc
      121 tatcagattt atctaatgta aagtttctat aagagtctga tgtcgtgtaa tcaatcacta
      181 aatgagtagg ggttagttga tagtgaatat cagggcaatt ttgttttaaa aagagagcca
      241 attgacgttc ccgctgtttt agataacgct taataaagcg aagatttttt tgaaacatcc
      301 cattttctaa ataaagagct aatgcttttt gcattagtaa gttcgtgtct aaatcaatca
      361 gtgatttttg ggttaaaaaa taaggtttta aacctgacgg taaaactaga gctccgatac
      421 ggagtgcagg aaaaacactc attgaaaaag attttaggta gatgattcgg tgatgagtgt
      481 cataataatg aattggtggc tctttaagtt tcacaaaatc tcctaaataa tcatcttcta
      541 aaatataaac ctggtatcgt tgtgctagac gaacaatagc ttccttttcc ttagtgctat
      601 aagaaagtcc cagaggatgt gaaaatcgtg aaatcgtgta aaaaaattta atatcgccag
      661 tttgaaataa agattctaag agttctaaat caaggccatt aaagtgtcta gagatagttt
      721 gatagggtaa tcctaactga gcaacaatag cttccatccg atgataagta ggcttttcaa
      781 ggagaattgt tttgcccgca ttaggaaaag gcatttgtga taaaatataa agcgcttgtt
      841 gcgtcccaga ggtgattaat agttggtctt tgttactgta aacactattt tcagctaagt
      901 aaagaagcaa agcttccctt agttcttcta gtccctcggt tttgtaataa taatgaaaga
      961 ggtatttgtc tttagcactt aaggcctcat ttaagcagag tcggaaatct tcataagcca
     1021 tattattata atcttcaaga cttagattaa gaacattatc gggcatgctt acgttaccca
     1081 aaacataata accactttta ggaacagcat aaattaagtg acgatatttt aattctaaaa
     1141 gagcccgctg aacagtatct ttgctacaat gataaacctt acttagtacc ctaattgaag
     1201 gtagcttgtc cccttttttt agtcgctgtt tctggatatc ttgttcgata ttggaaatga
     1261 ttgtttgata ttttgttgtc ataaattgtc cccatacagt taagtgttat aactattgta
     1321 tcttatgcct ctttttctta caatagttat tatagaaaag aggtaatagc tatgaaacgg
     1381 attattgtgg caaatgatct ggttggtgtt ggtaaggtag ctttgtctgc tagtattcct
     1441 ttaatggctt cttgtttgtt agagcaagtt ttattaccaa cttgcctttt atcaagccat
     1501 actggcggag gacagagccc tgtgaagcat actacatcaa gtatattaga cgttttttta
     1561 acagattggg acaacaaaag aattaagttt gacggcatct ttataggtta ttttacgaca
     1621 acaagagatg ggcaaaagct actagaatgg ctttcaagaa agcaactttc cctaatttta
     1681 gatccaatta tggcagataa tggaaaactt tatcaaggct tcagtcaaaa gcatatagag
     1741 attatgataa aacttactca aaaagctgag cttgttttac ctaatattag tgaggcttgt
     1801 ctattaacgc aaacagctta tttagcaaaa aattatgatg aagaagatat tagacaggtg
     1861 gctagaaaat tagctaagct tggacctaaa caggttgtca ttagcggaat ctcttttgag
     1921 gagggcagca ttggttttgc ttactatcag tcagattcag atcaattaac attccatttt
     1981 gggaagcaat tcccttacca tttctatggt acaggagata tagcatcagc tattataaca
     2041 gcaggattct attatcagct accactaaca caaattctag atcttgttgt ttcatttctt
     2101 gaaaaagtat tgcaaaatac ccttacatta aaaagagatt taatcttagg tgttgcttat
     2161 gaatctcatt tagattactt aactaaatcg tttaaaaagt tgttggagga aaaaaatgtc
     2221 atctcaaaaa ttagctaatt atattgaatt gagtatgtat gctgctttaa tttttatttc
     2281 agtacaagtg ttgcgtatcc ctgtcggtat gcagtttatt catcttggca atgcattagt
     2341 tgttattgca gtcttaatct atggtagtaa aaaaggagct ctagttgcta gtcttggttt
     2401 agggatcttt gatgttttga acggctatgc ttcagtcgtt tggataacta tcttagaatc
     2461 cttagctatt tgtttaattt tgcatttggt atatgaaaga gctatgcaat ctagtaaaaa
     2521 accaaaagcc attataacaa taacagttat tgctgcattg tcaaaattaa ctattaacct
     2581 tataaaatac ttactgttag cttatatagg tactcagcta ccactttcgg tatctttttg
     2641 ggtagctctt ggaaaaattg gtggtagttt cggaacatct tttgtgactt tagttgctgt
     2701 tcctcttttg tatcctgtta tcaaaaactt aaaagaaaag gttacaatcg gacaagcaaa
     2761 aagaggtttt tgatacaagt accttatcga taaaaaagca aggtacactt ttagtcaaat
     2821 attgacaaaa ggttacaatt ttgccataat gaaataacta taaagccgtt ttgacggctt
     2881 ttttgatgac agttaaggaa gaaacgatga gtattttaga agttaagcag ttgagttacg
     2941 gttttgggga tcgggctatt tttgaaaatg tgtcatttcg cctcttaaaa ggcgaacata
     3001 ttggactagt tggggcaaat ggtgaaggaa aatcaacctt tatgagtata gtcacaggac
     3061 atttacagcc tgatgaagga aaggtagagt ggtcgaagta tgtcactgca ggttacctgg
     3121 atcaacatac agtgttggaa tcaggacaaa ccgttcgtga tgtcttgcga actgcttttg
     3181 atgagttatt taagaccgag aatcgtatta atgagattta cgcgtcaatg gcagatgata
     3241 aagctgatat tgctgttttg atggaagaag taggtgagct tcaagaccgt ttagaaagtc
     3301 gtgatttcta tactttggat gctaagattg atgaagtagc gcgtgcgctt ggtgttatgg
     3361 attttggaat ggagtcagat gttacatcct tatcaggtgg gcaacgaaca aaggttttat
     3421 tagccaaatt actattagaa aaacctgata ttctgctatt agatgaacca actaaccatt
     3481 tggatgctga gcatattgaa tggttaaaac gctatttaca acattatgaa aatgcttttg
     3541 tgttgatttc gcatgatatt tctttcttaa atgatgtgat taatattgtt taccatgttg
     3601 aaaatcaaag tttagttcgc tatactgggg attattacca atttcaagct gtttatgaga
     3661 tgaaacaatc tcaacttgaa gcagcctatg aacgtcaaca aaaagagatt gctaacttgc
     3721 aggattttgt caaccgaaat aaggctcggg tagcgacacg taacatggca atgtctcgcc
     3781 aaaaaaaact tgataagatg gatataattg aacttcaagc tgagaaacca aaaccaaatt
     3841 ttgaatttaa gcaagctaga actcccagtc gattcatttt tcaaacaaaa aatcttgtga
     3901 ttggttatga ttacccattg accaaagaac ccttaaatat aacgtttgaa agaaatcaaa
     3961 aaattgctat tgttggggcc aacggtattg gaaaatctac tttgctaaaa agtttattag
     4021 gtgttatcga gcctttagaa ggtcatattg tcacagggga ttttttagaa gttggctact
     4081 ttgaacaaga agtgacaggt gttaaccgac aaactccgct agaagtagtt tgggatgctt
     4141 ttcctgcctt aaatcaggca gaagttcgag cggcactagc tcgttgcgga ctaacatcaa
     4201 aacatattga aagtcaaatt caagtacttt cgggtggtga acaagcaaaa gttcgttttt
     4261 gtttgttgat gaatcgtgaa aataacgtgc ttattttaga cgaaccaaca aatcatcttg
     4321 atattgatgc taaaaatgag ctcaaacgtg ctttaaaagc atataagggt tctattttaa
     4381 tggtttgtca tgaacctgat ttctacaatg ggtgggtaac cgatacttgg gattttagta
     4441 agttaaccta aactattcct taatttttta cacaggaatt actaaaatca gtaaagatat
     4501 agaataaata ctgctagata tgtgatggtt taggtaactg atcaccaacc aaaaaagatg
     4561 tgactacatc tttttttatc taatggaatg gtgtaaagtc gtttaataat agcttatttt
     4621 ttgatgatca gttgttcatg aaattgttgg tgattaacaa gtcatttaa
//
LOCUS       DATURV010000083         4403 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DATURV010000083 DATURV010000000
VERSION     DATURV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.083"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            57..770
                     /locus_tag="VCF75_001509"
     CDS             57..770
                     /locus_tag="VCF75_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP2547060.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKTIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            767..1678
                     /gene="pfkB"
                     /locus_tag="VCF75_001510"
     CDS             767..1678
                     /gene="pfkB"
                     /locus_tag="VCF75_001510"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEP2547061.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETELN
                     GQGPIISQEQLEELKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNAIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            1675..3621
                     /locus_tag="VCF75_001511"
     CDS             1675..3621
                     /locus_tag="VCF75_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2547062.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVAIGAIVSGILFGALRKKA"
     gene            3713..4312
                     /locus_tag="VCF75_001512"
     CDS             3713..4312
                     /locus_tag="VCF75_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP2547063.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
BASE COUNT         1311 a          744 c          977 g         1371 t
ORIGIN      
        1 aaacgatttc agttaggggt ggttctatct taaaatcaaa acgaaaacaa ttaatcatgg
       61 caaagatcac tgaagaaaac tatgtttctt tagaggattt aatgcaatta ctcaactcct
      121 ctgagtctac tatccgtaga gatctaggag agttggagca agaaggacgg ctacatcgtg
      181 ttcatggagg tgctgaactt tttcactctt tacaagagga actttctaac caagaaaagt
      241 ctgtcaaaaa cagtcatatt aaaaaaacta ttgcgcaaag agcctctcag tttatctatg
      301 acaacgatgt gatctttatt gatgctggga caacgactga atttttatta ccttttttac
      361 aagctaaaaa tttaactgta gttactaatt ctattcatca tgctgctaga ttagttgaac
      421 tctctattga gacgattatt gtagggggtt atgtcaaaca aacgaccgat gccagtattg
      481 gtaatgtagc tttggaacag attaggcaga tgaattttga caaggcattt cttggaatga
      541 atggtgttga tgattcttac ctgacgactc ctgatatgga agaagcagtt attaaaaaag
      601 cggttctgtc aaatgcaaaa ttagcttaca ttttagtaga tggcactaaa attggtcagg
      661 tatcgtttgt aaaagtagct cctattaatg atgtgactat tattacatta ggaggatcag
      721 caagtattct taaacaaata aaggaaaagg caaaggtgat tgaattatga tttataccgt
      781 gaccttaaac ccttctattg actttattgt cagaattgac caaataaatc ttgggtcagt
      841 gaatcgcatg gcgagtgatg ataagtttgc tggaggtaaa ggcattaatg tgagtcgcat
      901 cttgcaacgt ttagacattg ctagcacggc gacagggttc ttgggtggtt ttacaggacg
      961 ttttatcgaa gaaagtttga gtgcagaagg cgttaaaact gattttgtaa aaggtgatca
     1021 agatactcga atcaatgtta aaatcaagtc acaagaagag accgaattaa atggtcaagg
     1081 acctatcatt agtcaggaac aactagagga gttgaaaact aagctttccc agctaacagc
     1141 agaggataca gttgtctttg caggttcagc gccagctaat cttggcaatg ctgtttataa
     1201 ggaattactt cctttagtta ggcaaagcgg tgctcaggtg gtttgtgatt ttgaaggaca
     1261 gactttaata gatgcgctta catataatcc tctgttggtt aaacctaata atcatgaact
     1321 agaagctatt tttggtacga ttctgactag tcttgatgat gttgaaacct atgcccgtcg
     1381 tcttttagag atgggtgctc aaaatgccat catttcaatg gctggtgatg gtgctttgct
     1441 ggttactaaa gaagcgactt attttgctaa accgattaag ggagaagtta aaaattctgt
     1501 cggtgctggt gattcgatgg ttgctggctt tacaggcgaa tttatgaaga gtcaaaatcc
     1561 aatagaagcc ttaaaatggg gcgtggcttg tggtacagcg acggctttct ctgatgactt
     1621 ggcgacaatt gcatttatta aggaaacata tcataaagtt gaggtagaaa aacgatgaaa
     1681 attcaagatt tattgagaaa agatattatg attctcgatt tacaggctat ttctaaagaa
     1741 gttgccattg acgagatgat tactaaatta gttgaaaaag atattgtaca tgattttgat
     1801 gtctttaaaa agagtatcat gacacgtgaa gaacaaacat caacagggct tggtgatggg
     1861 attgccatgc ctcattctaa aaatattgta gtagataaac cagcggtctt gtttgcgaaa
     1921 tcaaataaag gcgtggatta taaggcttta gatggtcaac caacggatct cttctttatg
     1981 attgctgcgc ctcaaggagc aaatgatact cacttggctg cgctagcaga attatcacaa
     2041 taccttttga aagacggctt tgctgataaa cttcgtgctg cagcgacacc agaagcagtc
     2101 attgctgttt ttgatgaagc gtcaacagct aaagaagaag tggttgctcc aacaagtggt
     2161 caagacttta tcgttgctgt tacagcttgt ccgacaggaa ttgctcacac ttatatggca
     2221 gaagaggctt tgaaaaaaca agctgcagaa atgggagtag ctattaaagt tgaaacaaac
     2281 ggtgcttctg gtgtggctaa tcgtttaacc gctgaagata ttcagagagc taaaggagtt
     2341 attgttgcag ctgataaagc ggttgaaatg gaccgttttg atggtaaaca attcattgct
     2401 cgccctgttg cagatggtat caagaaaagt caggaattga tttctttgat tttaaacaat
     2461 gaaggaaaca cttatcatgc taaaaatgga aaatctgaaa cagcagtatc aactgagaaa
     2521 acaagtttag gcggtgcttt ctataaacac ttgatgggcg gtgtctcaca aatgttgcca
     2581 tttgttattg gtggcgggat tatgattgct ctagcatttt tattggataa catgcttggt
     2641 gtgccaaatg atcagcttgg aagtcttgga tcctatcatg aaatagcagc tatttttatg
     2701 aacattggtg gagcagcctt ttccttcatg ttaccagtac tagcaggtta tatcgcttat
     2761 tctattgctg aaaaaccggg cttagtagct ggttttgtgg caggtgccat tgcctcaaat
     2821 ggtcttgctt ttggtaaggt accatttgca gcaggaggag aagtgagctt gggcttaacg
     2881 ggcgtgccat caggtttcct tggggcgctt gtgggtggtt tccttgccgg tggtgttatt
     2941 cttgcccttc gtaaattgtt ggcaggccta ccacgttctc tagaaggggt taaatctatc
     3001 ctcctttacc cattacttgg cgtccttgtc actggtttct tgatgctttt tgtcaatatt
     3061 ccaatggcag ctattaacac agcccttaat gatttcttac aaggtctttc aggaagctct
     3121 gctgtcctta tgggacttct tgttggtgga atgatggctg ttgacatggg tggtcctgtt
     3181 aataaggcag cttatgtttt tggtacagga actttagcag ctactgtcgc aaatggcggt
     3241 tcagtcgtta tggctgctgt tatggcaggt ggtatggtac ctcctctagc tgtttttgta
     3301 gccactctct tgtttaaaga taaatttacc aaagaagaac gtgaatcagg tttgaccaac
     3361 attgtcatgg gactttcttt catcactgaa ggagcaatcc catttggcgc agctgaccca
     3421 gcgcgtgcaa ttcctagctt tattgctggt tctgccttga caggagctct tgttggtttg
     3481 gctggtatta aattaatggc gccacatggt ggtatcttcg tcatcgcctt aacaagtaat
     3541 ccaatcttat accttgtctt tgtagcgatt ggtgctattg tatccggaat cctcttcggt
     3601 gcccttcgta aaaaagctta acgttaaaag actttttcct tactaaaact agaatctgct
     3661 aaggttctag ttttttgata tactagagtt aaaggataag aaatgaggac ctttgaaggt
     3721 gaaaaaaagg agaagaaggg ctaaatcatc agtaaatcga cttgtcttag ggttagtatt
     3781 acttaatctg attgttagta tgtggacatt aaaattggga aaccagcgat tagctccgta
     3841 tgcggatcac gaaactctga catttgtcag gaagattagt catgctgctc aatcagtcgc
     3901 tcaaaagaaa cagttataca gttcggtgat gatggctcag gccattttag aatccaataa
     3961 tggtaagtca caactaagtc aaaaacctta ttataatttt ttcgggatta aggggagcta
     4021 taaagaacgg tcagtcattt ttccaacttt agaagacgat ggacaaggga atctttatca
     4081 aattgatgct gcttttcgtt cctatgggag cctgacagct tgttttttag attacgcaag
     4141 agttttgaat gatccacttt atgacaaaac ccacaaaaag ttttggtctc attatcaaga
     4201 tgctactgca accttaacag gcacttacgc cactgataca acttaccata ccaaattaaa
     4261 tgaattgatt gaatggtatc aactcactaa ttttgatggt ctaatgaaat agttatccac
     4321 aattttaaaa aaatcagaag aaatatgtcg atttcaagac aaaagttatc aatctcatta
     4381 aattatctgt ggaaaagtta att
//
LOCUS       DATURV010000084         4403 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DATURV010000084 DATURV010000000
VERSION     DATURV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.084"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            87..500
                     /gene="yaaA"
                     /locus_tag="VCF75_001513"
     CDS             87..500
                     /gene="yaaA"
                     /locus_tag="VCF75_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEP2547064.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRVGDKISLPDQDLIIIIVEPSQEEKEQFAEEMAEKTRVAALVKQMNQANKK
                     TSSKHNNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            502..1608
                     /gene="recF"
                     /locus_tag="VCF75_001514"
     CDS             502..1608
                     /gene="recF"
                     /locus_tag="VCF75_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEP2547065.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH"
     gene            complement(1664..2527)
                     /locus_tag="VCF75_001515"
     CDS             complement(1664..2527)
                     /locus_tag="VCF75_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEP2547066.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIVGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
     gene            complement(2729..4210)
                     /gene="guaB"
                     /locus_tag="VCF75_001516"
     CDS             complement(2729..4210)
                     /gene="guaB"
                     /locus_tag="VCF75_001516"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEP2547067.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT         1491 a          898 c          734 g         1280 t
ORIGIN      
        1 ggaatagtca aatagctctt attcagctat tattatttat ttctgtttta tgctataatg
       61 acttgaatga caaaatggag atgacaatga tatacaaatt atttacagaa tttatcaccc
      121 tacaagccct tcttaaagaa ttaggaatta tccaaagtgg tggcgctatc aagggatttc
      181 tcgctgaaac aaccgttcta tttaatggtg aagatgagaa acgtcgaggc aaaaaaatca
      241 gagtagggga taagatttct ctacctgacc aagacttaat tatcattatt gttgagccta
      301 gtcaagaaga aaaagaacaa tttgccgaag aaatggctga aaagacacgt gtagcggctc
      361 tagtgaaaca aatgaaccaa gctaacaaaa agacttcttc taaacataac aatcgtcaat
      421 ctactaccaa aaaatcacta agagcaacta agaaaactaa aggtaaacca actgcacctg
      481 tccgcttccc aggtatctag tatgtggatt aaagagttag agttaaaaca ttatcgcaat
      541 tatgaccacc tgcttgcctc attttcgtcg ggtttaaatg tctttatcgg taataatgct
      601 caaggaaaaa ccaattttct cgaggcaatc tattttctat cacttactag aagtcatcgc
      661 acgagggctg ataaagaatt gattcatttt gatcactcaa ctgtttctct tactggtaag
      721 attcagcgta ttagtggtac tgttgattta gaaatcaatc tatcagataa aggacgtgtg
      781 acaaagatta atgcgctgaa gcaagcaaaa ttatctgatt atattggaac gatgatggtt
      841 gtgctttttg caccagaaga tttgcaacta gttaagggag cacctagcct tcgtcgcaaa
      901 tttatcgaca ttgaccttgg tcaaattaag cctgtttact tatctgagtt atcccattat
      961 aaccacgtgc ttaaacagcg taatagctat ttaaaaagtg cccaacaaat tgatgccgct
     1021 tttctagctg ttctagatga gcaattagct ggctatggag cccgtgtcat ggaacatcgt
     1081 attgatttta tcaatgcgtt agagaaagaa gcaaatactc atcaccaagc catctcaaat
     1141 ggtttagaaa gtctctctct ctcctatcag tcgtcagttg tctttgacaa aaaaactaat
     1201 atttaccaac aatttttaca ccaacttgaa aaaaatcatc agaaagattt cttccgtaaa
     1261 aacactagcg ttggtccaca tcgcgatgac ttagcctttt acattaacgg gatgaatgct
     1321 aactttgcta gtcaaggtca gcaccgcagc cttattcttt ccttaaaaat ggctgaagtt
     1381 agtttaatga aggccttgac tggagataat cccattcttt tactagacga tgtcatgagc
     1441 gagctagaca atacacgaca aacaaagtta cttgaaacag taattaaaga aaatgtccaa
     1501 acctttatca caacaacaag tttggatcac ctgtcacagt tgccagaagg tatccgtatt
     1561 ttccacgtga caaaaggaac cgttcaaatt gattctgata ttcactaata acatcaaaaa
     1621 agggttcagt taaactgagc ctttttttga tattatgtgg cgattaagat ttgaccacac
     1681 ctaataaaat agcacctacg ataaaacaaa taattcctgt gacaacccaa cgcatttctt
     1741 ttttggtttt ggtttcgcct aggaataaaa tgcctccaac aatcgaaatg atagcaccta
     1801 attgtgagaa actaaaggca atagcaagtc ctgcttttga tgcagctaaa agcataaaaa
     1861 tattcccaat gccccaaagt aatccaacaa cgctattttt aatcacatac tggtcaatgg
     1921 aaattttaaa tgacatgaaa gttatagctc ctaaaaccat acctactgcc atgggtaaaa
     1981 tgactgacaa aacttcaaat ttcatgatgt tattaaataa tacagcatac atcacgtaac
     2041 caattgttga ataagtgagc gccctaaaac cttttgaaaa attatgaagg tgattgactt
     2101 gtgcattagc atcatcttgt ttactagaaa agtaaaaacc aacaatcaga agtaatagtg
     2161 ctagacttcc tactacaaat tgcataggtc ttgtccattc atgaaaaacg aggacaccaa
     2221 tcaaacttcc cagaacaagt tgagagccac ttgacaaagg attggcaacg gagactccca
     2281 tgtattgcat ggcatgaaat tgaccagttt gaccaattga ccagataaaa ccacccaata
     2341 ttccaaaaag ccaaagttgc agtgtcatct caggtctaac aattaaccaa acagctaatg
     2401 aaaataataa agccccaaaa gtcattccta gagtttgttg cgaaggtttt cctccaattt
     2461 tgttactaac aaatccaatg cttccccatg taaacatggg aataagtgca taaaaaatac
     2521 cttccaaaat gttatctcct ttatagtaaa tctttaaaga ttataataat tctatcataa
     2581 aatggtttca tgtaaagact aattatagct tatttactaa gaaaaaacaa taaaagttat
     2641 ccgaaaattg ttattatatt ttatcatttt ttaatataaa aaagagctgg cacaatatgt
     2701 cagcccttaa agttactaaa ttaaattatc agtgcacaga atagtttggt gcttcatttg
     2761 tgatttggac atcatgcgga tgactttcaa tcaagcctgc acctgacatt tcaacaaatt
     2821 gagcattttc atgtaattct tgaatgtcac ctgcgccaac atagcccata cctgagcgga
     2881 taccacctaa catttggaag acaatatctg aagcagctcc tttataagca acacggcctt
     2941 caataccttc tggaactagt ttattagctt catttacgga accttggaag taacgatcgc
     3001 ttgatccttt cttcatagct gcaatggaac ccattccacg ataagttttg aacttacggc
     3061 cttggtagat ttcagtttca cctggagctt catcagtacc tgcaaacatt gatccaagca
     3121 taacagcatt accgccggct gcaagagcct taacaatatc gccagaatac ttgataccac
     3181 catcagcaat aatcgttttg ccatattcac gagcaacagc agcagcatcg taaatagcag
     3241 tcacttgagg aacaccgaca cctgcaacca cacgagtggt acagatagat ccgggaccaa
     3301 tacccacttt aacaacatca acacctgcat cataaagagc acgcgcacct tcagcagtgg
     3361 caatatttcc agcaatcaaa gtacgatttg ggaaatgagc acgaatttca gcaatttttc
     3421 taagaacacc agccgaatga ccatgagctg tgtcaataac aatcgcatct gcaccagctt
     3481 caaacaaggc ctcggcacgc tcaaaagtat ctgaggtaac acctaccgca gcagcgacca
     3541 gaagacggcc aaattcatct ttagcagcat gtgggaattc gattactttt tcaatatctt
     3601 tgatagtgat taaaccagac agacgaccgc tattatcaac caaaggaagt ttttcaatac
     3661 ggtgttcatg aagaatacgt tctgcagttt caagatctgt accaacagca gccgtaacga
     3721 ggtgctcact agtcatatgt tcagagattg gtgcattgta atcagaaata aaacgcatat
     3781 cacgattagt aatgataccg accaacttac ggtttgctaa agtttcaaca attggcacac
     3841 cactgatacg gtagcgctgc attaattctt cagcttctga aaccttgtgt tcaggtgtta
     3901 agaagaatgg atcaatgatg acaccatttt cagaacgttt taccttgcga acttcttctg
     3961 cttgctcagt aatagacatg tttttatgaa taacacctaa accacccgca cgcgcaattg
     4021 caatagccat tttactacca gtaaccgtat ccatagctgc tgtaataata ggaatattta
     4081 atgtcaaatt gtctgcgagt tttgtcttta aatcaacttc atttggaaga acatgactct
     4141 ctgccggaat aagcaagaca tcgtcaaaag tgtagccttt tttcaaaaat ttagtgtccc
     4201 aatttgacat ccagataatc ctcttttctt ttatttttag ggaatattac cctggtctat
     4261 tttttattgt tactatcata tcacgctaaa atcatttgtc aataaaatat tcctacagct
     4321 gaagtcacta tcagcataat atatcagtat tatccgtttt ttattaatca aaaaattcat
     4381 ttgggtctta tttttaatca aaa
//
LOCUS       DATURV010000085         4397 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DATURV010000085 DATURV010000000
VERSION     DATURV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.085"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            1..801
                     /locus_tag="VCF75_001517"
     CDS             1..801
                     /locus_tag="VCF75_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEP2547068.1"
                     /translation="MGLTIFKKLVSLIILLLFFAILKRVTNYLFEKTINKSFAYSRQS
                     EARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAGIAGVAIGLGAQGFLS
                     DVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFDGTLHFIPNRSITVVS
                     NKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIVGKPNILGPQNNSNGQ
                     FTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYSITSKS"
     gene            complement(866..1426)
                     /locus_tag="VCF75_001518"
     CDS             complement(866..1426)
                     /locus_tag="VCF75_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEP2547069.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERYVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(1440..1910)
                     /locus_tag="VCF75_001519"
     CDS             complement(1440..1910)
                     /locus_tag="VCF75_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2547070.1"
                     /translation="MQNSKIRQMSLTGILTALIVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGLKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            complement(1900..2664)
                     /locus_tag="VCF75_001520"
     CDS             complement(1900..2664)
                     /locus_tag="VCF75_001520"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEP2547071.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIISLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            complement(2654..3403)
                     /gene="truA"
                     /locus_tag="VCF75_001521"
     CDS             complement(2654..3403)
                     /gene="truA"
                     /locus_tag="VCF75_001521"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEP2547072.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            complement(3590..4171)
                     /locus_tag="VCF75_001522"
     CDS             complement(3590..4171)
                     /locus_tag="VCF75_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP2547073.1"
                     /translation="MHNEKNNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLRRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGERFKGRRALLRKISPYFKEFAQQL"
     gene            complement(4167..4240)
                     /locus_tag="VCF75_001523"
     tRNA            complement(4167..4240)
                     /locus_tag="VCF75_001523"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4204..4206),aa:Arg,seq:acg)
     gene            complement(4320..4393)
                     /locus_tag="VCF75_001524"
     tRNA            complement(4320..4393)
                     /locus_tag="VCF75_001524"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4357..4359),aa:Asn,seq:gtt)
BASE COUNT         1394 a          971 c          645 g         1387 t
ORIGIN      
        1 attggcttaa cgatttttaa aaagctagtg tctctcatta ttctattact cttttttgct
       61 attttaaaac gtgttactaa ctatcttttt gaaaaaacca ttaacaaatc gtttgcttat
      121 tcaagacaaa gtgaagctcg caaaaaaaca ctctctaagc tcactcacaa cattttaaat
      181 tacttattat attttcttct catttattgg atacttagct tatttggcat acctgtctct
      241 agtcttttag caggagctgg aattgctgga gtagcaatcg ggttaggtgc acagggattt
      301 ttatctgacg ttgtcaatgg cttttttatc ttatttgaga atcaatttga agtaggagat
      361 aacgttacta tctctgacat agaaggcagt gtctttggtg ttggaattag aactactcaa
      421 attcgtggat tcgatggaac actacatttc atcccaaacc gtagcatcac cgttgtcagc
      481 aacaaatctc gtggtaatat gcgtgcctta attgaaattc ctctttattc cactgttaat
      541 ttgagccaag tcactcgaat cattgatgaa gtgaatcaaa aggaattgcc aaaccatcct
      601 caaatcgttg gcaaaccaaa tattttaggg cctcaaaata actctaatgg acagttcact
      661 tttagaattg ctattttcac agaaaatgga gaacaattta agatttacca tactttttat
      721 agactttatc aagaagcttt actcaaagaa ggcattcaat taccgactgc aatcagctat
      781 tctatcacct caaaatctta atataatctt aaaaaatcct tttttctaga aaaaacacca
      841 attgttttat tccagaaaaa agggtttagg atttacggat aatatccata ttgtaatcag
      901 ccctagctcc accaattaat tttggtcgac tagccaaagc tgtcacatga gcacctccta
      961 gctctcgttg acaaggaatc agagggatct gaacacgctt aatatgcatt cctatagcag
     1021 tatcacctat atcaagtcca gcgtgtgcaa taacttcttc tacttcgaca gggtctgaca
     1081 taaattgaaa agcagccatc tgagcactgc caccagcatg aagattcgga accacattga
     1141 caatctctaa ctgcttagat tctgccacat acctctctac taccaaggca cgattgacgt
     1201 gttcacaacc ttgtacagcc aaatgaacac cacgcttatt taattcatct aacacaactt
     1261 caacaactat ttgacctact tctagactag attgttttcc aattctacta cccaaaattt
     1321 cgctagatga aagacctaga acaaaaagat tacctggttg aatagcagag cgttctacaa
     1381 catcaatgac gatttccctt gtttgtttct ctaaattatt caacaacttc tcactctcct
     1441 tatctctttt tttgccttaa tatggcctta aagataataa aaccaacaaa aagccctaat
     1501 gtattttgca tgacgtttcc ccaaataccg gctaaagatc ccgaaaggcc ataaccaagc
     1561 ataagagagc ctaaaaagta ccaaaaaatc atgataaaag aacctatcac aacaccaagc
     1621 caacgcttga gccctctcca tcccgcaaaa tacccttgga cactgtgagc aatcaaacta
     1681 tgaaacatcc actgagggta tcctgccact aaatcgatta aaaaacctga taaccctccc
     1741 acaatagctc cttgagctga tccaaaggaa aagctgacag cataaattcc tgcatccaac
     1801 agcgttaaaa aacctgttgg agtaggtagc atgacaaatc ttcctaaaac aacaatcaat
     1861 gctgtcaata tccctgttaa actcatttgt cgtatcttac tattttgcat aactttgttt
     1921 tactccgtat ctatcagatt gtaaaatagc ttgatagact aactcttttg agtttttcac
     1981 agcttctagt ggcgtttttt tctttactaa ctgactagcg atactagatg caaaagtaca
     2041 gcctgcacca atattatttt tctcaagtac aggacattcc aaggtaacaa tctctttacc
     2101 gtcataaaaa agatcaatag cctttttctg actaaatcga ttaccaccct tgataaccac
     2161 ctgttttgcc ccaagttgat aaaaatattt agcagcttct tgcatatctt ttaaagaaat
     2221 aatctctttt tgagataata gctgagcctc taccaaattt ggagtaacaa cagttacata
     2281 aggcagtaat tgtaaaatct cctgtcttaa aggaactatt ttaacatcat ctatttcttt
     2341 acatgctaga acaggatcta aaaccactgg gattcccaga tgccccttta taaaatccaa
     2401 aactatttca cacatttccg cattaggtaa caaaccaatt ttaatagcag aaattggagc
     2461 gttcgtaaag ctgttcaact gatcacggaa aatttcttta gctaccggaa atagactaaa
     2521 gccctcttca gatctagtag ttaaacaagt tactgccaca aaggcctgta ggtcatacct
     2581 tatgtaagtc gctaaatcag catagagacc acctccactt aaaatatcgt tcccagatat
     2641 agtcacgata taatcagttt tcataacaaa tctccttcaa gtaaagccca tttccagata
     2701 tcgttggtcc agctagctga cgatttttgg agctcaaaat cactttaact tgttcaactg
     2761 gcatttgacc attaccaatt ttcagcaaag ttcccaccat attcctcacc tgcttataga
     2821 gaaaaccatt tcctgaaaaa gtaaacacca aaaaatcagt tttttcatcc cgagataccg
     2881 ttgctttggt aatcgttcgc accttatttt gtaccgaagt tcctgctgct gtaaaaccag
     2941 taaaatcgtg tgtacctacc agaccgttaa tagcttcttg cataagttta atattaagag
     3001 tataagggta atgagtggta tagtgtcgca tcatcggatt tttaggtcga ccattatcaa
     3061 ctaaaaattc ataagttttt aaatgcttct gataacgaca atgaaaatca tcagccactt
     3121 tttctatatt aacaacatca atatcttcag gagtctgtgt atcaagagca aatcgtaatt
     3181 tttcaacttc ttgttcttga ggcaaatcaa aatggatcac ctgtccataa gcatgtactc
     3241 ctgcatccgt acgtcctgca ccatgaatta taatttttgt accgttattt aatttataga
     3301 gtgttttttc aatctcttct tgaaccgtac gtaaatgtct ttgtctttga aaacccgaaa
     3361 aaagagtccc atcataggaa atcgttgctt tgtatcttac catgttacta ttttaacatt
     3421 tcttaaacgt aagagaaact atttttaatc aaactgtacc caaacaaatg aaagacgtat
     3481 tgaagcttac tctaagctac taacgattta aaagctataa gaaagctatt tcctccaaac
     3541 tctctctcct ttttttatag atttaccccc aattccttat aggtcttgct tacaactgct
     3601 gtgcaaattc cttaaaatag ggactgatct tcctaagtag agcacgtctt cccttgaaac
     3661 gttcgcccct gataagtgct tgaaactgca tacgctcttg cgaatttaat tggttctcta
     3721 agctagctat tacctcttga taagccacat agtcgtctag ccataaccct ctcgatggaa
     3781 tggcatgtgc aacctcccct atctcttcat atgctaactt atggaactga cgcttttgac
     3841 tttcttgacg gcgtaataaa tctttcaaat aagatgaaaa ttttgtttta aaatagcgat
     3901 ataaacgctc ttcttcctca attaactctg ggtacctttc tagtaactgt aataagataa
     3961 tatgtccttc ttgtaaccag tcatctctat cccacaattg aatataatag tgtcgcttta
     4021 atttcaaaat aatgggctta actttttcaa acgctgctct tgtctctatc gacattttga
     4081 gtctcctttt ctttttaact atccctagta taaagagaac cacaaaaaaa caacaggaca
     4141 ctaatgtcct attgtttttt tcattatgca ccctagagga gtcgaacctc taaccgcctg
     4201 attcgtagtc aggtactcta tccagttgag ctaagggtgc caacgcatat attgcctcta
     4261 tcttaccaca ctcttctcta aaatgcaaaa aaagatagga cgcttcctac ctctttctac
     4321 tccgccagta ggactcgaac ctacgacatc atgattaaca gtcatgcgct actaccaact
     4381 gagctatggc ggataaa
//
LOCUS       DATURV010000086         4396 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DATURV010000086 DATURV010000000
VERSION     DATURV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.086"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            35..2308
                     /locus_tag="VCF75_001525"
     CDS             35..2308
                     /locus_tag="VCF75_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEP2547074.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKKGDYYMV
                     ITLPEDLSQRATTLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMTKLKESVSQNITKI
                     YTSAVFRSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEELSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     SLQSLSQEDQSKQLEQLKEAVAQIANQSNQALPGASSALTELSTGLAKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTAIGSGVIKLSEGTNALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(2424..3503)
                     /locus_tag="VCF75_001526"
     CDS             complement(2424..3503)
                     /locus_tag="VCF75_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEP2547075.1"
                     /translation="MKNISRKCFITSVLCIILGGILLGAGYATGGLQDIKHQTAPKKV
                     IKTFDQITALDIDSSASTITVETGPVQRPTVTYYTHPKFIDPIVTTVTGKTLSLSQKP
                     KDVVITGGIEILGFTLNNSRQEKNYRSITITVPEKTSLNEVKGSNVPHTTLSNLTVQD
                     MQFDGNLTLLHTKVKKATITGMLEATKSQLTNLELKADYSFSNLTDSSVENGTISLGN
                     GQLTTKDTTLKAVNIQSLHPGGIEAERTTLENVTFTVSKSKEEEENDYYDNDAIFTAH
                     ALTLKGTNTITGGDIDVDITLTKAKAIAYRARTENGKVSLGSQLTPAKIGKESTSDVI
                     SYVAENKAATGNLTVNLNKGDITIK"
     gene            complement(3500..4087)
                     /locus_tag="VCF75_001527"
     CDS             complement(3500..4087)
                     /locus_tag="VCF75_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEP2547076.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATAILRFSGRAFVAFIKWISNKKGAQA"
     gene            complement(4074..>4396)
                     /locus_tag="VCF75_001528"
     CDS             complement(4074..>4396)
                     /locus_tag="VCF75_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP2547077.1"
                     /translation="FPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLYPI
                     LKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGSLR
                     HDKD"
BASE COUNT         1308 a          843 c          897 g         1348 t
ORIGIN      
        1 atttttgaac agtatgtcca ttttaggaga aaatatgtta gaagaattga aaacacttat
       61 taaaaatcca aaattaatga ttacaatgat tggtgtggcc ctagtgcctg ccttatataa
      121 tttatccttt ctaggctcaa tgtgggatcc ttatggtcgg gtcaatgacc ttcccattgc
      181 tgttgttaat catgataagc ctgcaaagag agctgataag tcattgacaa ttgggaatga
      241 tatggtggac aagatgtcta aaagtaaaga tttagagtat cattttgtct cagctaagca
      301 agctcaagag ggtcttaaaa aaggtgatta ttatatggtc attactttac ccgaagatct
      361 ttctcagcga gcaacaacct tattaaatcc tgaaccccaa aaactaacta tccgttacca
      421 aactagtaaa ggacatggaa tggttgctgc taagatgggt gaaacagcga tgacaaaact
      481 gaaagagtct gtttcgcaaa acattacgaa gatttacacc tcagcagttt ttaggagtat
      541 gacagacctc caatcaggat taaaagaagc ctcagctgga agtcaggcac ttgcttcagg
      601 agcgaagacc gctcaggcgg ggagtcaaac actttcgacg aacttagcag ccttaacggg
      661 tgctagccaa cagtttcaac aaggtactgg tcgattgaca tcaggtttga ctacctatac
      721 agatggtgtc aaccaagtca agaatgggtt aggaacatta tcaacggaca tccccaatta
      781 tctgaatggg gtttctaggt tatcccaggg agcttctcag cttaatcagg gtctttcaca
      841 gttgacacaa gcaacaacac tttctgacga gaaagctaag ggaattcaat ccttaattgt
      901 aggactacca gtcctaaatc aaggcattca gcaactaaat acagagctat caacattgca
      961 accccctaac cttaatgctg atgagttagg taatagctta ggagctatcg ctcaagctgc
     1021 caaacaagtc attgctgaag agactgccgc tcagaatgaa gaattatcgg ctctccaagc
     1081 tactagcgtt taccaatcat taactgctga acaacaagga gagttagctg cggccctcag
     1141 tcaatctgat aaaagtcaga ccgtatctgc agcccaaact attttaagtt ctgttcaaac
     1201 tttgtcaaca agtttacagt ctctctctca agaagatcag tcaaaacagt tggagcaact
     1261 taaggaagct gttgcacaga ttgctaatca atccaatcaa gctttgccgg gagcaagttc
     1321 tgctttaact gaattatcaa cgggattagc aaaggtaaat ggtagcttaa atcaacaagt
     1381 tctaccagga agtaatcaat tgacaacagg attagcacaa ttaaacaggt ataatactgc
     1441 cattggttct ggggtaataa aactctcaga aggtaccaat gccttgtcat ccaagtccgg
     1501 agaattacta gatggtagcc atcaattatc agagggtgct actaaactag ctgatagtag
     1561 ttctcaattg agtcagggtg gtcatcaatt aacgagcgga ttgactgaat tatcaacagg
     1621 attatcaacc ttaaatggtt ccttagccaa agcctctcag cagttatcgc ttgtttctgt
     1681 gactgataaa aatgctaaag ctgtcgcaaa acctcttgtg ttaaatgaga aagacaaaga
     1741 tggtgttaag acgaatggga tcgggatggc accttatatg attgctgttt ctctaatggt
     1801 tgtggccctt tcaaccaacg tcatttttgc taattcttta tctggtcgtc cggtcaaaga
     1861 taaatgggat tgggctaaac aaaaatttgt tattaatggt tttatttcga ctatgggatc
     1921 cattgttctc tacttagcta ttcaattatt agggtttgaa gcccgttatg gtatggaaac
     1981 cttaggattt attatgctaa gtggttggac gtttatggct cttgtcacag ctttggtcgg
     2041 ttgggatgat cgatatggct cttttgcttc tttggtcatg ttattgcttc aggttggctc
     2101 ttcaggtggc tcttacccca ttgagttaag tggagcattc ttccaaaagt tacatccttt
     2161 cttaccaatg acttatgtgg tatctggttt acgacaaacc atttcattat caggtcatat
     2221 tggagtagaa gtgaaagtct taactggttt cttactggca tttatggtat tagcactact
     2281 catttatcgt cctaagaaaa cagtctaaat aaaataacct caaaaaaaac agcatctaag
     2341 gcagttacac tagatgctgt tttttgtctt attttgacaa taacttgaca atttctgtca
     2401 agttttttat tgtcttgtta agatcatttg atagtaatgt ctcccttatt gagattaacc
     2461 gttaaatttc cagtagctgc tttattctca gccacataag aaataacatc tgaagttgat
     2521 tccttaccaa tcttagctgg tgtcagctgt gagccaaggg agactttacc attttcggtc
     2581 cttgctctgt aggcgatggc ctttgctttt gtcaaggtta tgtcaacatc aatatcacca
     2641 ccagtaatag tattagtccc tttaagggta agggcatgag cggtgaagat agcatcattg
     2701 tcatagtagt cgttctcctc ttcttctttg cttttagaaa cagtgaaggt cacattttca
     2761 agggttgttc tctcggcttc tataccacca gggtgtaatg attgaatatt gacagctttt
     2821 agagtggtat ctttagtagt tagttgtcca tttcctaagc tgatggtccc attttccaca
     2881 ctagaatcag tcaggtttga aaaagaatag tcagctttta actcgagatt tgttaactga
     2941 cttttagtgg cttccaacat accagtgata gtagctttct tgactttggt atgtaaaaga
     3001 gtaagattgc catcgaattg catatcttgg acagttagat ttgacaaagt ggtatgtggg
     3061 acattacttc ccttaacttc attgaggcta gttttttcag gaacagtaat ggtaatagaa
     3121 cggtagttct tctcttgacg actattattg agagtaaaac ctaaaatttc aattccacca
     3181 gtgatgacaa cgtctttagg tttttgcgaa agagataggg tcttacctgt tactgttgtg
     3241 acgatagggt caataaattt aggatgtgtg taataggtca ctgttggtct ttgaacgggt
     3301 cctgtctcta ctgtaatagt tgaggcagaa ctgtcaatat caagagcagt tatttgatca
     3361 aatgttttga tgaccttttt gggagctgtt tggtgtttaa tgtcctgaag tcctccagtt
     3421 gcatagccag cccctagaag aatgcctcct agaataatac ataacacact ggttataaag
     3481 catttgcgtg agatattttt catgcttgag ctcctttctt attactgatc cacttaataa
     3541 aggcaacaaa agcccgtcct gaaaaacgaa ggattgctgt ggcaataatc caaataagaa
     3601 gaccaccacc aaaacacaac aaggcgctgc cgatgcccat agcttgtgca ggaatagatg
     3661 agccaatcaa ggtcaagctt tcaaacagag tcactcctgc gacaagaata cctgcaagcc
     3721 caaaaatgta ggcggcaaag atagctgtga gcccgagcat taggcaggca ataataaaga
     3781 gaatcaacgc cagcaatacg ggtaaagcaa tcggagcagc aaagagggac aaaatggtta
     3841 atccaataat tgttgcccga tttttgggag ttttctcggg agttgccaag tgttttccta
     3901 gaacatttga aatgatttca ctggcagctt ccttaggatt tcctaaatcg tcgataacct
     3961 ggctttcttt gtcaggacca gcttcatcaa aatattcaat ataatattcc atcgcttcat
     4021 ggtagtcttc tctaggtagt ttgcgcaagt atttatctag ttctgctaga tactcagtcc
     4081 ttgtcatgtc ttaagctccc ttcaataatc ccgtctaagg caaatttata gctctgccat
     4141 tcttttttta gaaaaatcag ctgtgctctc cccgaagaag tgacagcata atatttacgc
     4201 tttcgtccct gatgttcttg actataagtt gtcaaaaaac ctgccttctc caatttttta
     4261 agaataggat agagggtgga ttctttgata ttagccacta gtttaatggt ttgactaata
     4321 tcgtaaccgt aagaatcgtt tttttcgaca atggctaaaa tcagaaattc aatcaaggta
     4381 gccgatactg gaaaat
//
LOCUS       DATURV010000087         4318 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DATURV010000087 DATURV010000000
VERSION     DATURV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.087"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            114..1139
                     /locus_tag="VCF75_001529"
     CDS             114..1139
                     /locus_tag="VCF75_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2547078.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIAILGTVAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            1153..1695
                     /gene="pgsA"
                     /locus_tag="VCF75_001530"
     CDS             1153..1695
                     /gene="pgsA"
                     /locus_tag="VCF75_001530"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEP2547079.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            1697..2536
                     /locus_tag="VCF75_001531"
     CDS             1697..2536
                     /locus_tag="VCF75_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP2547080.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            2512..3354
                     /locus_tag="VCF75_001532"
     CDS             2512..3354
                     /locus_tag="VCF75_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP2547081.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELKEV
                     LKHG"
     gene            3347..4147
                     /locus_tag="VCF75_001533"
     CDS             3347..4147
                     /locus_tag="VCF75_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP2547082.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
BASE COUNT         1382 a          686 c          835 g         1415 t
ORIGIN      
        1 tacaattgga aaagcatttt ttgaaaaagc tgatgcttca gacttcacag tcttcccaaa
       61 atagatgaaa atctgttata atagggttac tatattattt ttgaggtacc tgtatgagtg
      121 aaaaaagtct tggtgaagtg ttaagagaat cgcgtgttgg aaaaaatatc acactagatg
      181 atatagaatt gaagacaggg atttcttccc attacttgct ggcaatggaa ttggatcagt
      241 ttaaaattat tccggaagaa aaatttgatc aatttttaaa agaatatgct gacattgttg
      301 agttagattt caatacttta aaaagacgat accgttatca agttaattcc aaaaaagaca
      361 gtgatataaa atctgtaact gagattgttg aagagaagtt aagtaagaaa cgattacaag
      421 agaagttgtt atcacatcag agcatggggt atggtcaacc gttagaagtt tctaaaaaca
      481 aagaaacctt agttatccct gtaaagactg ttggtttaga tagcaaatcg ccactaaaaa
      541 cgatatcgcc tgttagccaa tttaaatcag ataacaggtt aattcctaaa aaaaatgtgt
      601 cacgtatgag gcgaaatagc actgacagtc gttctaaaac gtcactattt cccatagcta
      661 tattaggaac ggttgctttt gcaattatca gtgttatcat tttaggtgtt tggcaacaat
      721 tcgaaaagag tcaaagagct aaagaagctg aagttgcttt gcttaaagcc tctgagcagt
      781 ctactaacgg tttaaaaaat aattcaaatg atcgcaaaac tcaagtcaca actgaggggt
      841 cgggtaatta tttaatagcg actgttacta aatctaaaga gaccgttgat atcagtgtgt
      901 ctttaactga ggcacaaagt agttggattt ctctaacaaa ttcagaaatt ggtgaaggtg
      961 gtataacatt gactcaagac tcgccaactt atacagcgac tttacctgca gaaataacag
     1021 aatcattgtt gacacttggt gtgacaaaag gtgtatctgt aacaattgat ggacaacctg
     1081 ttgatctatc acctttaaca agtactgact taagttatat cacgtttaag atccaataat
     1141 taaggtagac taatgataaa aaaagaaaat attcctaatc tattaactct tgttcgaatc
     1201 gcaatgattc ctttctttct ttttattact tcctcatcta ataaggtggg gtggcatatt
     1261 tttgcagctg tgatttttgc aattgccagt tttaccgatt atctagatgg gtatcttgcg
     1321 cgtaagtggc atgtagccag taattttgga aagtttgccg atcctctagc agataagatg
     1381 cttgtcatga gtgcctttat catgttagtt ggacttggct tagttcctgc gtgggtgtca
     1441 gctgttatta tttgccgaga attggcagta actggtcttc gattactact tgttgaaacc
     1501 ggaggaaagg tccttgcagc tgctatgccg ggaaaaatca aaacagcgac acaaatgttg
     1561 tctattattt tattactttg ccattggata ttcctaggaa acgtcttact ctatattgct
     1621 ctttttttca ctatttattc tggatatgat tattttaaag gagcaagctt tctttttaag
     1681 gatacgttta aataacatgt cagctattat tgaacttaaa aaagttacat ttaattacca
     1741 taaagaccaa gaaaaaccaa cattagatgg cgtatcgttt catgtgaaac aaggtgagtg
     1801 gctgtctatc attggccata acggatctgg taaatcaacg actattcgat taattgatgg
     1861 attattggaa ccagaatcag ggtctattat tgttgatgga gatctactaa ccataactaa
     1921 tgtttgggaa attcgtcata aaattggtat ggtctttcaa aaccctgata atcagtttgt
     1981 tggagcaact gttgaagatg atgttgcttt tgggcttgaa aataaaggta ttgcacatga
     2041 agatataaaa gagagggtta atcatgcttt agagttagtc ggcatgcaga actttaaaga
     2101 aaaagaacca gcccgtttat ctggtggcca aaaacagcgc gtagctattg caggtgcagt
     2161 tgctatgaag cctaaaatta ttattttaga tgaagctact agtatgcttg accctaaagg
     2221 acgattagag ttaatcaaaa ctataaaaaa catccgtgac gactaccagc tgactgttat
     2281 ttccattact catgacttag atgaagttgc tcttagtgat agagttttag tgatgaaaga
     2341 tggtcaagtg gaatcaacat caacaccaga acaattattt gcaagagggg atgaactact
     2401 ccaattaggt cttgatatcc cttttacaac atctgttgta cagatgcttc aagaagaagg
     2461 ttatcctgtt gactatggat atctcacaga aaaggaatta gaaaatcagt tatgtcaatt
     2521 aatctccaaa atgtaagtta tacttatcaa gcagggactc cttttgaagg gcgtgccctt
     2581 tttaacatta atttagatat tttagatggt tcttatacgg cttttatcgg acatactgga
     2641 tccgggaaat cgaccattat gcaattgcta aatggtttac acgttccgac aacgggaatt
     2701 gtcagcgtag ataaacaaga tataacgaat cactctaaaa ataaagagat caaatctatt
     2761 cgaaaacatg taggattagt ttttcaattt ccagaaagtc aactatttga agagacagtt
     2821 ttgaaagatg ttgcttttgg ccctcaaaat tttggggtct ctccggagga agcagaagct
     2881 ttagcgcgtg aaaaactagc tcttgttggg atttcagaaa atctttttga aaagaatcca
     2941 tttgaactct cagggggtca aatgcgtcgt gttgctattg cgggtatcct tgctatgcag
     3001 ccaaaggttt tagttttaga tgaaccgact gctggtcttg atcctaaagg gcgtaaagaa
     3061 ttaatgacta tttttaaaaa gttacaccaa tctggcatga ctattgtttt agtaacccat
     3121 ctgatggatg atgtggccaa ttacgcagat tttgtatatg ttcttgacaa aggtaaaatt
     3181 attttatcag gaaaacccaa aacaattttt caacaagtca gtttgcttga gaaaaaacag
     3241 ttaggagttc ctaaggtgac taaacttgct caaaggcttg ttgatagagg aatacctatc
     3301 tcttctttgc ctattacctt agaggagcta aaagaggtac taaaacatgg ataagctgat
     3361 tcttggtcgt tatattcccg gagattctct cattcatcgt cttgacccaa gaagtaaatt
     3421 attagcgatg attatttata ttgtcattat cttttgggct aacaatgttg tgacaaattt
     3481 gttgatgttg acttttactt tagcagttgt ttttttgtca aaaatcaagt tgtctttctt
     3541 tttaaatggg gttaaaccaa tgattggtat tatcctcttc acgactttat ttcagatgtt
     3601 tttctcacaa ggaggaaaag taatttttag ttggtggttc attagcataa ctgatttagg
     3661 attgagccaa gctattttaa tttttatgcg tttcgttcta attattttct tttcaacgct
     3721 gttaacgttg acgacaacac ctttaagcct atctgatgca gttgaatcat tattaaaacc
     3781 tttaacaaga tttaaagttc cagctcacga gatcggacta atgctttctt taagtttacg
     3841 ttttgtgcct actttaatgg atgataccac gcgtattatg aatgcacaaa gggcccgtgg
     3901 agttgatttt ggagagggaa atttaattca aaaagttaaa tccattattc ctattttaat
     3961 ccctcttttc gcttcaagct ttaaaagagc cgacgcatta gctattgcta tggaagctag
     4021 aggttatcag ggaggagaag ggcgaactaa atatcgtcaa ttagactggc agctaaaaga
     4081 tagtttagct attggaatag tttctttatt gggtttgcta ctttttttct tgaaaacctc
     4141 tttgtaatat tttttagcac aaaacttttc tttattataa gaagtgaatt gtacctcaaa
     4201 agttagatac aacatctaat gattgaggta cttttcttca tttgaggaat tgaaaaccgc
     4261 tatctcaact atattaatcc tcccaataat aacaaatcga acgaaatcta aaaggact
//
LOCUS       DATURV010000088         4292 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DATURV010000088 DATURV010000000
VERSION     DATURV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.088"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..787
                     /locus_tag="VCF75_001534"
     rRNA            <1..787
                     /locus_tag="VCF75_001534"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            839..911
                     /locus_tag="VCF75_001535"
     tRNA            839..911
                     /locus_tag="VCF75_001535"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ala,seq:tgc)
     gene            1210..4112
                     /locus_tag="VCF75_001536"
     rRNA            1210..4112
                     /locus_tag="VCF75_001536"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4198..>4292
                     /gene="rrf"
                     /locus_tag="VCF75_001537"
     rRNA            4198..>4292
                     /gene="rrf"
                     /locus_tag="VCF75_001537"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1208 a          892 c         1216 g          976 t
ORIGIN      
        1 tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa
       61 cgatgagtgc taggtgttag gccctttccg gggcttagtg ccggagctaa cgcattaagc
      121 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca
      181 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac
      241 atcccgatgc ccgctctaga gatagagttt tacttcggta catcggtgac aggtggtgca
      301 tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaacccc
      361 tattgttagt tgccatcatt aagttgggca ctctagcgag actgccggta ataaaccgga
      421 ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta
      481 caatggttgg tacaacgagt cgcaagccgg tgacggcaag ctaatctctt aaagccaatc
      541 tcagttcgga ttgtaggctg caactcgcct acatgaagtc ggaatcgcta gtaatcgcgg
      601 atcagcacgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga
      661 gagtttgtaa cacccgaagt cggtgaggta acctattagg agccagccgc ctaaggtggg
      721 atagatgatt ggggtgaagt cgtaacaagg tagccgtatc ggaaggtgcg gctggatcac
      781 ctcctttcta aggaaatgga acacgtttat cgtcttattt agttttgaga ggtcttgtgg
      841 ggccttagct cagctgggag agcgcctgct ttgcacgcag gaggtcagcg gttcgatccc
      901 gctaggctcc atcaggatac aatcctacta aacttaatac aagtgaagtt gaacacgcaa
      961 ctcacttcct aggaaaatag acaatcttcc cttgtgtgca aggcccacat ggtcagattc
     1021 ctaattttct acagaagttt cgctaaagcg agcgttgctt ggtatcctat ataatagtcc
     1081 attgaaaatt gaatatctat atcaaattcc acgatctaga aatagattgt agaaaagtaa
     1141 caagaaaata aaccgaaaac gctgtgacta tttaataagt tttctagttt aaaaaaacta
     1201 ggttaataag gttaagttaa taagggcgca cggtggatgc cttggcacta gaagccgaag
     1261 aaggacgtga caaacgacga aatgctttgg ggagctgtaa gtaagcgctg atccagagat
     1321 gtccgaatgg gggaacccac taactaatgg ttagtatcca taactgttaa ggttatgaga
     1381 aggaagacgc agtgaactga aacatctaag tagctgcagg aagagaaagc aaacgcgatt
     1441 gccttagtag cggcgagcga aacggcagga gggcaaaccg aagtgtttac acttcggggt
     1501 tgtaggactg cgacgtggga ctttaaaagg atagaagaat tacctgggaa ggtaagccaa
     1561 agagagtaaa agcctcgtat ttaaaattct tttgagccct agcagtatcc tgagtacggc
     1621 gagacacgtg aaatctcgtt ggaatctggg aggaccatct cccaacccta aatactctct
     1681 agtgaccgat agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg ggaggggagt
     1741 gaaatagaac ctgaaaccgt gtgcctacaa caagttcgag cccgttaatg ggtgagagcg
     1801 tgccttttgt agaatgaacc ggcgagttac gatatgatgc gaggttaagt tgaagagacg
     1861 gagccgtagg gaaaccgagt cttaataggg cgatttagta tcatgttgta gacccgaaac
     1921 catgtgacct acccatgagc aggttgaagg tgaggtaaaa ctcactggag gaccgaacca
     1981 gggcacgttg aaaagtgctt ggatgacttg tgggtagcgg agaaattcca aacgaacttg
     2041 gagatagctg gttctctccg aaatagcttt agggctagcg tcgatgttaa gtctcttgga
     2101 ggtagagcac tgtttgggtg aggggtccat ctcggattac caatctcaga taaactccga
     2161 atgccaacga gatatcatcg gcagtcagac tgcgagtgct aagatccgta gtcgaaaggg
     2221 aaacagccca gaccaccagc taaggtccca aaataactgt taagtggaaa aggatgtggg
     2281 gttgcacaga caactaggat gttagcttag aagcagctat tcattcaaag agtgcgtaat
     2341 agctcactag tcgagtgacc ctgcgccgaa aatgtaccgg ggctaaaaca gtttaccgaa
     2401 gctgtggatg acacaaaagt gtcatggtag gagagcgttc tatgtgtgaa gaaggtgtac
     2461 cgtgaggagc gctggaacgc atagaagtga gaatgccggt atgagtagcg aaagacaggt
     2521 gagaatcctg tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg ccctgggtta
     2581 gtcgggacct aaggagagac cgaaaggtgt atccgatggc caacaggttg atattcctgt
     2641 actagagtat atagtgatgg agggacgcag taggctaact aaaccggacg attggaagag
     2701 tccggctaag cagtgaggtg taagatgagt caaatgctta tctttataac attgagctgt
     2761 gatggggagc gaattttagt agcgaagtta gtgatgtcac actgccaaga aaagcttcta
     2821 gcgtttaatg atactctacc cgtaccgcaa accgacacag gtagtcgagg cgagtagcct
     2881 caggtgatcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta acttcgggag
     2941 aaggggtgct gacttaggtc agccgcagtg aataggccca agcaactgtt tatcaaaaac
     3001 acagctctct gctaaatcgt aagatgatgt atagggggtg acgcctgccc ggtgctggaa
     3061 ggttaagagg agggtttagc gcaagcgaag atctgaattg aagccccagt aaacggcggc
     3121 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg
     3181 aaaggcgtaa tgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt
     3241 gaagatgcag gttacccgcg acaggacgga aagaccccat ggagctttac tgcagtttga
     3301 tattgagtat ctgtaccaca tgtacaggat aggtaggagc cattgacttc gggacgccag
     3361 tttcgaatga ggcgttgttg ggatactacc cttgtgttat ggctactcta acccagatag
     3421 gttatcccta tcggagacag tgtctgacgg gcagtttgac tggggcggtc gcctcctaaa
     3481 gagtaacgga ggcgcccaaa ggttccctca gattggttgg aaatcaatcg cagagtgtaa
     3541 aggtataagg gagcttgact gcgagagcta caactcgagc agggacgaaa gtcgggctta
     3601 gtgatccggt ggtaccgaat ggaagggcca tcgctcaacg gataaaagct accctgggga
     3661 taacaggctt atctccccca agagttcaca tcgacgggga ggtttggcac ctcgatgtcg
     3721 gctcgtcgca tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc
     3781 ggcacgcgag ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt
     3841 aggaaatttg agaggatctg ctcctagtac gagaggacca gagtggactt accgctggtg
     3901 taccagttgt cttgccaaag gcatcgctgg gtagctatgt agggaaggga taagcgctga
     3961 aagcatctaa gtgcgaagcc cccctcaaga tgagatttcc catgatttta tatcagtaag
     4021 agccctgaga gatgatcagg tagataggtt aggagtgtaa gtgtagcgat acatgtagcg
     4081 gactaatact aatagctcga ggacttatcc aaaaagaaat attgacaacg ttacggattc
     4141 ttgttagact atagatattc aattttgagt gggttatgaa aatagcacat ttacaagtta
     4201 agtgacgata gcctaggaga tacacctgta cccatgccga acacagcagt taagccctag
     4261 aacgcctgat gtagttgggg gttgccccct gt
//
LOCUS       DATURV010000089         4233 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DATURV010000089 DATURV010000000
VERSION     DATURV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.089"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..181
                     /locus_tag="VCF75_001538"
     CDS             <1..181
                     /locus_tag="VCF75_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2547083.1"
                     /translation="YAKLIHPYLTTFDINVENLGKMSFKQLLDIINSNEQNLSQTIFV
                     PFSLKQRESVRDISH"
     gene            297..1790
                     /gene="malQ"
                     /locus_tag="VCF75_001539"
     CDS             297..1790
                     /gene="malQ"
                     /locus_tag="VCF75_001539"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEP2547084.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVADGYLQEADLVGVDFGSDPEVVDYA
                     QIFQARRPLLEKAVQAFLATKEGIKELAAFEAEASWLGDFAEFMSLKEHFQNKALQEW
                     DDRAIVKREEAALHHYRELLAKKITYHKVCQYFFYQQWSALKIYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            1825..4089
                     /gene="glgP"
                     /locus_tag="VCF75_001540"
     CDS             1825..4089
                     /gene="glgP"
                     /locus_tag="VCF75_001540"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEP2547085.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNSA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSTAEVEDVELEPSLGNGGL
                     GRLASCFIDSIASLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNYWIEDQSWLVPTNI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEDVDYGLIKDGISFDKTEIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     CLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
BASE COUNT         1382 a          751 c          824 g         1276 t
ORIGIN      
        1 ttacgctaaa ttaattcacc cttatttaac gacatttgat ataaatgttg aaaatcttgg
       61 aaaaatgtct tttaagcaat tacttgatat tatcaattcc aatgaacaga atcttagtca
      121 gactattttt gttcctttta gtttaaaaca aagagaaagc gtccgtgata ttagtcatta
      181 agagcaaaaa aaagtagcat ttttaaggaa tgttactttt tttaggaaac tggttgactt
      241 acgcaagcgc ttgcgttata atctcatcat gaatagattt cacaaggaga aaaatcatga
      301 acaaacgtgc aagcggtatc ttaatgcata ttagttccct gcctgggaaa tttgggatag
      361 gcacttttgg aaaatcagct tttgaatttg ttgattttct agcagagacg aaacaaacct
      421 attggcaaat tctgccttta acaacgacga gttttggaga ttctccttat cagtcatttt
      481 cagctattgc tgggaacaca catttcattg attttgaatt acttgttgca gacggctact
      541 tgcaagaagc tgatctcgtt ggcgttgatt ttggttctga ccctgaggtg gttgactatg
      601 ctcagatttt tcaggcaaga cgccccttat tagaaaaagc ggtccaagcc tttcttgcaa
      661 ctaaagaagg catcaaagaa ttggcagctt ttgaagcaga agcgagttgg ttgggtgact
      721 ttgccgagtt catgtcattg aaagaacact ttcaaaataa ggccctacaa gaatgggatg
      781 acagagcaat tgtgaaacgc gaagaagcag ccttgcatca ttaccgtgag ttgcttgcaa
      841 aaaagattac ctatcataaa gtctgtcaat acttctttta ccaacaatgg tcagccttga
      901 aaatttacgc taaccataaa gggatagaaa ttattggtga tatgcctatt tatgtttctg
      961 cggacagtgt agaagtttgg acgatgcctg agctatttaa agtagatagt gataaaaagc
     1021 cattgtttat tgcaggtgta ccagcagatg gctttagtga agatggtcag ctgtggggaa
     1081 atccaaccta caattggtct gctcatgaaa agtctaattt tgcatggtgg atttatcgta
     1141 ttcaagaaag ttttaaacta tatgatcagc taagaatcga tcattttaaa ggcttctcag
     1201 atttttggga aattcctgct ggtgataaaa ctgctagaaa tggccattgg gcctcagccc
     1261 caggtatagc attattttct gcggttcgtg aggccttagg agagcttcct atcatcgcag
     1321 aaaaccttgg ttatattgat gaaaaagcag agcagctatt agcatcaaca ggctttcctg
     1381 gcatgaagat attagaattt ggtttatttg atataacgag tcaaagtata gatttgccac
     1441 attactatga ccgtaattgt gtcgcttaca cagggactca tgataacgag gtggttaacg
     1501 gatggtatga caatcttagt gaagaacaag tacactttgt aaataactat cttcacaaac
     1561 atgcagatga aagtattaca aaagccatgc taagaacaat ctttgcttct gtttgtgata
     1621 cagccattct ttgcatccaa gatttattag ataaagatgg taagagtcgt atgaatatgc
     1681 caaatacaat tggaggtaat tggcaatggc gtatgcttga cggtgaattg aaccaagatc
     1741 acaaagatta ccttatttat ttaacagatc tttatggtcg cgcaagataa tatttaaatg
     1801 ttacattatt gaaaagagga agacatgaca cgctttacag aatatgttga aactaaacta
     1861 ggaaaatcac ttacacaagc cagcaatgaa gagatttatc tatcattatt aaactttgtc
     1921 aaagaagaag ctagccacaa ggctaaaaat tctgctaaac gcaaagttta ctatatctca
     1981 gcagagtttt tgattggtaa attactatca aacaacctga ttaacctagg aatttacaaa
     2041 gacatcaaag aagaattggc agctgctggc aaatctaccg cagaagtcga agatgttgaa
     2101 ttagaaccat cactagggaa tggtggttta ggacgtttgg catcatgttt cattgattct
     2161 attgcatctc ttgggattaa tggtgaaggg gttggtttaa actatcattg tgggttgttt
     2221 aagcaagtct tcaaacataa tgagcaagaa gctgagccaa actactggat tgaagaccag
     2281 tcatggttgg ttcctacaaa catttcatat gatgtgccct tcaaaaactt taccttaaaa
     2341 tctcgtcttg atcgtattga tgttttgggt tacaaacgtg acactaaaaa ctaccttaat
     2401 ttatttgaca tcgaggatgt tgattatggt cttatcaaag atggcatttc ttttgataaa
     2461 acggaaattg ctaaaaactt gaccttgttc ctctacccag acgattctga taaaaatggg
     2521 gaattgctcc gtatttacca acagtacttt atggtgtcaa atgcagcgca attaatcatt
     2581 gatgaagcta tcgaacgtgg ttctaacctt catgatcttg cagactacgc ttacgtgcaa
     2641 attaatgaca cgcatccatc aatggtcatc ccagaattaa ttcgtctctt gactgaaaaa
     2701 catggctttg actttgatga agcggtagct gttgtgaaaa atatggttgg ttacactaac
     2761 cacactattc ttgcagaagc ccttgaaaaa tggccaactg cttacttaaa cgaagtagtg
     2821 ccacacttgg taaccatcat tgaaaaattg gatgctcttg ttcgttcaga agtgtctgac
     2881 ccagctgttc aaattattga cgaatcaggt cgtgtgcaca tggcccatat ggatattcat
     2941 tttgcaacaa gtgtcaatgg ggtagcagca ctccacacag aaatcttgaa aaacagcgaa
     3001 ttaaaagctt tctatgacct ttacccagaa aaattcaaca acaaaaccaa cgggattact
     3061 ttccgtcgtt ggctagaatt tgctaaccaa gacttggctg attacattaa agaacttatt
     3121 ggcgatgagt acttgactga cgcaacaaaa ctagaaaaat tgatggcctt tgcagatgac
     3181 aaagctgttc atgctaaatt ggctgaaatc aaattcaaca acaaattagc tcttaaacgt
     3241 taccttaaag acaataaaga cattgagctt gatgaacatt ctattattga tacccaaatc
     3301 aaacgtttcc acgagtacaa acgtcaacag atgaatgctc tttacgtgat tcacaaatat
     3361 ttggaaatta aaaaaggcaa ccttccaaaa cgtaaaatca ctgttatctt tggaggtaaa
     3421 gcagcgcctg cttacatcat tgctcaagac atcattcact tgatcctttg cttgtctgaa
     3481 ttgattaaca atgaccctga agtaagccca taccttaatg tgcatctagt tgaaaattac
     3541 aacgtgacag tagcagagca cttgattcca gcaactgata tttctgagca aatttcacta
     3601 gcatccaaag aagcttctgg aactggtaat atgaaattca tgcttaacgg tgctttaaca
     3661 cttggtacaa tggacggtgc taacgtagaa atcgctgagc ttgcaggcat ggaaaatatc
     3721 tacacctttg gtaaagattc tgataccatc atcaaccttt atgcaactgc ttcttatgta
     3781 gcaaaagatt actatgataa ccaccctgct attaaagcag cagtgaactt tattatcagt
     3841 ccagaattgc tagcatttgg caacgaagaa tgtcttgatc gtctttataa agaattgatt
     3901 tcaaaagact ggttcatgac cttgattgac cttgaagagt acattgaagt gaaagaaaaa
     3961 atgttagcag actatgaaga ccaagattta tggatgacaa aagtggtcca taacatcgca
     4021 aaagctggat tcttctcatc tgaccgtacc attgagcagt ataacgaaga tatttggcat
     4081 tcacgataaa aaataataat tgcaatgtgg ggtagcggta ccgtaacatg cttaaactaa
     4141 attataagac agtagtttct gctgtcttat gatttttctt cagacttagg ttaattatta
     4201 cattaagcgt aaagcaagtc tgtttatcga tct
//
LOCUS       DATURV010000090         4204 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DATURV010000090 DATURV010000000
VERSION     DATURV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.090"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(70..1092)
                     /gene="trpS"
                     /locus_tag="VCF75_001541"
     CDS             complement(70..1092)
                     /gene="trpS"
                     /locus_tag="VCF75_001541"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEP2547086.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
     misc_binding    complement(1147..1388)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1511..2383
                     /locus_tag="VCF75_001542"
     CDS             1511..2383
                     /locus_tag="VCF75_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP2547087.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMTSNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     gene            2462..4081
                     /locus_tag="VCF75_001543"
     CDS             2462..4081
                     /locus_tag="VCF75_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP2547088.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATDDSILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
BASE COUNT         1306 a          778 c          736 g         1384 t
ORIGIN      
        1 acaaattagg tcctgctttg ataatagtaa ttatcttatc attactaact tagctttttc
       61 tctaaaacat taaaaataat taatacccat tgctgacttc acttctgata aagtcttggc
      121 tgccacagtt cttgcttttt gactaccttc ttgtaacata cggaacacct ctcccatatc
      181 tttagcgtac tccaaacgtc tttcacgaat aggtgctaat tcacgttcta aaatatctag
      241 aaggtagcgt ttcgttttca catctcctaa accacctctt tgataatgct ctttcattgc
      301 ttcgatatca gcttgatctt cttttctagc aaaaatatcc aaataatgaa agaccatatt
      361 cccttcaatt tgaccaggat cttctatttt aatatgattt ggatcagtat acatgctcat
      421 cacttttttg cgaacggtat ctgcatcatc tgagagatag attccatttc ccaatgattt
      481 agacattttg gcattgccat caagaccagg taagcgtcca gctttttcat tttctggata
      541 aataccttca ggttctacta aacagtctgt atggtaagta tgattaaaac ttctcacaat
      601 ttcacgtgtt tgttcaatca tcggtttctg gtcgttacct acaggtacta aattcgcttt
      661 aaatgctgta atatcagcgg cctgtgatac tggataaacc aaaaaaccag atgggatact
      721 ttcgccaaaa cctttctgag caatttcggt tttaacagtg ggatttcttt ccaaacgtgc
      781 taatgatacc agattcatat aatacatgct caattcagct agctctggaa tctgactttg
      841 aataaaaatc gttgattgct tcggatccaa accgacagag aggtaatcca aggcaacatt
      901 tccaatagat tcttgaatta attcggattc tttagcatga tccgtcagtg cctgttgatc
      961 ggccaagaaa acaaacatct tatacttgtt ttcattttgt aaaaagacac gatttttaag
     1021 acttccgaca taatgaccta aatgtaattt tccagttggt ctatctccag ttaaaataat
     1081 aggttttgtc atcattttct ccctaactat ctcttgatat agcaaaaagt ccccacgcta
     1141 ttaaaacgtg agggcgttat tacaagtaat acgcggtacc acctcaatta gcatctgctt
     1201 tatcaaaacg agatactatc tcaatcttct caatgaaaga agctgcacgg taaggggtgc
     1261 caacctgaaa gttatggttt cctttcaaat aatcattagt ccatttcacc ttttctgttg
     1321 ttagcttgca ccaaccacta actctctaaa aacatgaaaa gaatactctt ctaatttaga
     1381 tttattctat gaaattttaa acttcttgtc aagttcacca gattttttga aaatatgact
     1441 tgacggtctc agtacattta agagataatg agtctactat ttttaaccag ttataaagga
     1501 gattaaaagc atgaaaaaac gagggattga tttactactt gtcacaattg gctcattcat
     1561 aacagctatt ggtttcaata caatgtttgt cgacaatcat attgcatcag ggggtatggt
     1621 tggtattgca gttgttataa aagctctatt tggcattagt ccgtcgcttt ttttaatgac
     1681 tagtaatatc cccttattat taatgtgtta cttctttctt ggaaaacaaa attttattaa
     1741 aacactttat ggttcttgga tatatcctat tgccatccgt tcaaccaatt cattacctac
     1801 tctgacacac aatcagttgt tagcagctat ttttggtggt attatctgtg gcattggttt
     1861 aggaatggtt ttttggggaa actcttcgac aggaggaact ggaattctaa cacaaatact
     1921 tcataaatac tctcctttat ctcttggagt tgcaatgaca attgtagatg gtattagtgt
     1981 ccttatggga tttattgcac tttctgcaga tgacgtgatg tattctacga ttggactttt
     2041 cgtaattggt tatgtgatta gcgttatgga aaatggcttt gactcatcca aaaatgttat
     2101 gattatttcc aaagattatc aagctattcg agaatatatc acaactgtta tggatcgtgg
     2161 agtgactaaa cttcctattc gcggtggtta tactacttct gataaaatta tgttaatggc
     2221 tattgtatca agtcacgaat tacctactct ccaagaaaaa atcctcgaaa ttgacgacac
     2281 agcctttatt gttgtcatgc cagcagctca agttatggga agaggattta gtttgactaa
     2341 acaatacaaa cgtgaagaca aagacgtttt attgcctatg tagtgattag cccttttctg
     2401 attgaaaaag gggactattt taggtacaat gaagcttaga taaataaaat gaggtgacaa
     2461 attgcttaca gtttctgatg tatcactacg ctttagtgat cgaaaactat ttgatgatgt
     2521 taatatcaaa ttcacagccg gcaatactta cggcttaatc ggtgcgaatg gtgccggaaa
     2581 atcaactttt cttaaaatcc ttgcaggaga tattgaacca agtactggtc atatctcttt
     2641 aggacctgac gaacgcttat ctgttcttcg ccaaaatcat tttgattatg aagaagaacg
     2701 tgcgattgat gttgtaatca tgggtaatga gcaactttac aatatcatga aagaaaaaga
     2761 cgctatctat atgaaagcag atttttcaga agaagatggg gttcgtgccg ctgagctaga
     2821 aggtatcttt gctgaattag gtggctggga ggcagaaagt gaagcttctc aacttctcca
     2881 aaatctaaat attccagaag accttcacta ccaaaatatg agtgaactgg ctaatggaga
     2941 taaagtgaaa gtccttcttg ctaaagcgct ctttggtaag cctgacgttt tattacttga
     3001 cgagccaacc aatggtcttg atattcaatc tatttcatgg ttggaagatt tcttgattga
     3061 ttttgaaaat acagtcatcg ttgtttccca tgaccgtcac ttcttgaata aagtatgtac
     3121 tcacatggcc gaccttgact ttggtaaaat caaactcttt gtgggtaact acgatttctg
     3181 gaaacaatct tctgagctag ccgctcgttt acaagctgac cgcaatgcta aagccgaaga
     3241 aaaaatcaaa gaattacaag aattcgttgc ccgtttctca gcaaacgcat ctaaatcaaa
     3301 acaagcaacc tctcgtaaaa aaatgcttga taagattgaa ttagaagaaa ttgttccctc
     3361 aagccgtaaa tacccattta ttaattttaa agcagagcgt gaaatgggaa atgacttcct
     3421 cactgttgaa aatctttctg taacgatcga tggagagaaa atcattgata atatcagttt
     3481 cattttacgt ccaggtgaca aagctgctat tattggtcaa aatgatattc aaacaaccgc
     3541 tcttatgcgt gctttagcag atgatattga ctacgaagga accattaaat ggggagtgac
     3601 aacaagtcgt tcttacttgc caaaagacaa ttcaaaagat tttgcaacag atgattccat
     3661 ccttgaatgg ttacgacaat tcgcctcaaa aggtgaagat gatgatacct ttttacgtgg
     3721 tttcctagga cgtatgcttt tctctggaga cgaagttaaa aaatctgtta acgttctttc
     3781 cggtggcgaa aaagtgcgtg tcatgctttc taaattgatg ctattgaaat caaacgttct
     3841 tattcttgat gacccaacta atcacttaga tttggaatct atttcaagcc ttaatgatgg
     3901 tatcaaagac ttcaaagaat ctgttatctt tgccagtcat gaccatgaat ttattcaaac
     3961 gattgctaac cacatcgttg ttatttcaaa aaatggtgtt atcgaccgta tagatgaaac
     4021 ttatgacgaa ttccttgaca atccagaagt ccaagcccga gttgctgaac tttggaaata
     4081 aaaccttatg ataaatgagg tggctaagaa gatctcttcc tagcctcttt tttaaaattt
     4141 gtaaacgaaa gaccatataa tttatgaaaa ataataataa atggataatt gctggacttg
     4201 ctag
//
LOCUS       DATURV010000091         4204 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DATURV010000091 DATURV010000000
VERSION     DATURV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.091"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(70..1674)
                     /locus_tag="VCF75_001544"
     CDS             complement(70..1674)
                     /locus_tag="VCF75_001544"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEP2547089.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            complement(1931..2506)
                     /locus_tag="VCF75_001545"
     CDS             complement(1931..2506)
                     /locus_tag="VCF75_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEP2547090.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEVEEDEEPVL"
     gene            complement(2723..4006)
                     /locus_tag="VCF75_001546"
     CDS             complement(2723..4006)
                     /locus_tag="VCF75_001546"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEP2547091.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDTSKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
BASE COUNT         1217 a          922 c          649 g         1416 t
ORIGIN      
        1 ttaatagcta gtaatcttta tcagtatctg ccagccaagt agaatgccac aaacttaccc
       61 ttaaaaccac taattgctat ttttaatagc agctgtaaca aaagcggtgt atagctcctc
      121 aggacgattt ggacggcttt gcaattctgg gtggtattga gctgctacaa agaatttttt
      181 ctctttcaat tctacaattt caactaagcg attgtcaggt gatacccctg agaaaacaaa
      241 accagctgct tcaaattctg gacggaattt gttgttgaac tcataacggt gacgatgacg
      301 acgttgcacc acctcttgat tattgtaagc cattgctgct tttgaacctg gtttcaattt
      361 acatgggtac aatcccagac gaagcgtacc tcccatatct tcaatatcaa tttgatcacg
      421 catgatatca atgatggggt atttagtact tggctctaat tcaaaagagt ttgcaccttc
      481 catattgagg acatggcgag caaattcaac acaagtcaac tgcatgccaa gacagatacc
      541 aagcattggc acatcattct cgcgggcata gcgaatcgct tggatcttgc cttctgtacc
      601 acgttgacca aaaccacctg gtacaataat cccatccgca tctcccaaca agtcagctgc
      661 attatccacc gtcacatcgt tagcattgac ccattttagg tcaattgcgg tatcattggc
      721 ataaccagaa tgtttcaagg cttccacaac agataagtag gcatctggca attccacata
      781 tttaccaacc aaggcaattt ttgttgtttt tcttaaattc atcaccttgt caaccatagc
      841 tgaccactcg gtcatatcag cttgtggcgc attcaatttc aaatgatcac aaacaatctg
      901 atccattgat tgagcttgca agttcaatgg aatttgatac aagtgttcta catcacgtga
      961 ctcgataaca gcttctgaat tcacatcaca gaactgagcc aatttatttt taatcccctg
     1021 ctcaactggc tcttccgtac gaataaccaa catattcggt tgaatcccta aaccacgcaa
     1081 ttctttaacg gaatgttgcg ttggcttagt tttcatttca cccgctgcct ttaaatatgg
     1141 caacaaggtg gtgtgaatat acatcacatt ttcagaacca acatcagctt tcatctgacg
     1201 aagagcttct aagaacggta aactttcaat atcgccaaca gtccccccga cctcagtaat
     1261 aattacatca gagtccgttg ttgaagctgc gcgtttgatt ttttctttaa gggcatctgt
     1321 gatatgagga atcacctgaa cagtcgctcc aaggtactcg cctttacgtt ctttacgaag
     1381 gacttcacta taaattttac ctgttgtaac attggaatat ttattaagat tgatatcaat
     1441 aaaacgttca tagtgaccca aatccaagtc ggtttcagca ccgtcatctg tcacatatac
     1501 ttctccatgc tgataggggc tcatcgtccc tggatcaata ttaatataag gatcaaattt
     1561 ttgaatcgtc accttaagtc cacgattttt taacaatcgg ccaagacttg ccgcaacaat
     1621 tcctttgcca attgatgaca caacaccacc agttacaaaa atgtatttcg tcatagtact
     1681 cctttatata agctgaataa aagcattgct ttcttgttaa ttccctatta gttggttcca
     1741 cattggttag gtatcacctt agttttctaa atatagaggc aaactggcat tattctagaa
     1801 aacaaaaata gccccctatt aactagagag cttttgcctg acctcataaa gaggtgcccg
     1861 aataataata taaactataa tagacaattt gtcaaatgat aattacattt ttatttctga
     1921 actaaagaca ttagagaact ggttcttcat cttcttcaac ttcttcgtct tcttcattga
     1981 tatcaacttc ttcaaaatca tcttcaggga taatttcatt caattctgaa tcatatgact
     2041 ccacttctga tttttcatca tctggatctt cttcatcata ttcaacctca gctgattctt
     2101 ctgtgaaatc ttcatcttct ggatcatcat ctctataatc aatagcgtct tcatctccat
     2161 ccatgaaagc attaacgcgt tttttcttac gtttttgtgc gccatcttca tcttcttcta
     2221 gagtaataat ttcttcatca atttcatcaa tagcatacca cgaacgaagt ccccatttgt
     2281 tttcacctag aggaataaat gagccatccg tatttaagtc agtatagaaa aatggtaatg
     2341 catgacgaat acctgcatca gattttccta agtaattttg aatctcattg actaaatcgc
     2401 taaagtacat ctcattgtcg cgaccgcgtt cttccaaaat ggcacgcgcc acttcaatca
     2461 tggaaagctc gcttttttct tgtcctgcaa atacgtctaa tttcaaggca gttctcctta
     2521 ttttatcatt ctctaccatt ttacgctaaa aaaagtaatt cgtcaaaact tttatcaact
     2581 catatcactt cttcttgaca aatctttaaa gctaattcat taaaataacg tttaactaat
     2641 tccttattat ttctttagaa aaagaaaaac cagactagaa agacgttcta atccggcttg
     2701 tacgttttgt tccttaataa gattacttaa cgcttgctgt gcttgtaatc acttcaactg
     2761 cttttttcat tgcaatatca tgtttcaaca tatctgctga aagaagagaa cgaacttggt
     2821 cagctggcat gttatattct gttgcaaggt cattaatttc ttgttcaatt tcactatctg
     2881 ttgcttcaaa accttctgct ttagcaattg cttcaataac aaggtttgtt ttaacacgtt
     2941 tgtcagcttc agcagaatat tggttatgta aatcttcttg agttgtacca gtcaattgga
     3001 agtacatttc aggtgagatt ccttgacgtt gcatgttgcc cataaactca ttcactgaac
     3061 ggttgacttc ttcatgaatc atttcttcgg gtaaatcaac aatttcagca tttgcaactg
     3121 ctagttcaat cgcagctcct tcaacagcat catcataagc agtttcttga gctgcttcaa
     3181 gttctttacg atattttact tttaagtctt caagtgtgtc aacatcttca tcaatatctt
     3241 ttgcaagctc atcatcaagc tctggtactt cttttgtttt gacttcgtga atagttgtca
     3301 taaatttagc ggctttacct gcaagatctt ctgcttggta agattctggg aatgtgacat
     3361 taacttctac ttcatcgcca gctttagcac caactagttg atcttcaaaa cctgggataa
     3421 attgtcctga accaagttca agagagaagt tatctccttt accgccatca aactcaacac
     3481 catcaactga accaacaaag tcaatcacaa cagtgtcacc ttgagctgct tcaccgtctt
     3541 taataatgag ttccgcaagg ttttgacgtt cgcgctcaat tttagcatcc acgtcttcat
     3601 ctgaaacttc ttttgaagta tcaacttcta caacaaggtt tttgtaatca ccaagtttca
     3661 cttcaggttt tgtcacaact tcagcagaaa gtgtccactc tttccctttt tccattgaca
     3721 caacgtcgat ttttggttgt gcaaccacat caagaccaag ttccgtcaca gctgcttcat
     3781 aagcttctgg caatacaata ttcaaagcat cttcataaag aacttcttca ccaaattttt
     3841 ggttgaagac tggacgaggc atgtgtcctt tacggaaacc tggtgcattc aagtcttttt
     3901 tgattttatt aaaagcttta tcaagagctg gttttatttt atcttgactg attgtaaatg
     3961 taatcacgcc acgatttgta gctttgtttt caaatgatgt agacattaag tcatttctcc
     4021 ttaaaataat ttcgatacag ttcattttat cataatttaa agcgttttta aagcttttta
     4081 tgcgtgaaat caaacgcgct attaagagta tctacctgtc attagtctac taacagttat
     4141 tttcaattta taaaggttaa tgttagactt tttcgcttac taactgacta ttttcaagca
     4201 ttat
//
LOCUS       DATURV010000092         4174 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DATURV010000092 DATURV010000000
VERSION     DATURV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.092"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(67..430)
                     /locus_tag="VCF75_001547"
                     /pseudo
     CDS             complement(67..430)
                     /locus_tag="VCF75_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(851..1384)
                     /locus_tag="VCF75_001548"
     CDS             complement(851..1384)
                     /locus_tag="VCF75_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEP2547092.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1464..2240)
                     /gene="recX"
                     /locus_tag="VCF75_001549"
     CDS             complement(1464..2240)
                     /gene="recX"
                     /locus_tag="VCF75_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEP2547093.1"
                     /translation="MKITKIEKKKRLYLIELDNDESLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2355..3710
                     /gene="rlmD"
                     /locus_tag="VCF75_001550"
     CDS             2355..3710
                     /gene="rlmD"
                     /locus_tag="VCF75_001550"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP2547094.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGENIFC
                     QITAVKRNFAEAKLLTVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT         1241 a          685 c          885 g         1363 t
ORIGIN      
        1 tagtaatgga tggtatggag atcttaatgt ggttttaatg gtaattttag cagttatagt
       61 aagacaaaag gtgacttgga aaagcttatg cagacgattc ttaactccca caatatcttc
      121 tgtcagattt tattagactt cttctcagtg tcaattttgt ctgttttacg aacgtgcagg
      181 ctattcaatt gcttcttagc ttctaaagga ttaaaccgag tataagcgta gccattatct
      241 gcgaaaaaac ctgaagacga tgagaataga cattaatcgc tttaaggata atctcagggt
      301 tatggacggt tttcaagttt ctaagaagac gattgaggcc gatggtatcg ttttgtcttg
      361 aatgattcct tattttagtg gtttcatttt caatacacga cgtcaagcgt cctacatact
      421 ttgataaaat tctacctaaa agaggtgact tgtcagtttt gattacaaca tcaagtgcca
      481 aaagactttg cgacttaaca agacacttct actttaacgc aaagaaaaag tagtgagtac
      541 tctctttcgt cggagatttc ctcgctacta aacttagtat tttttgatac tttccttagg
      601 tagtgcttaa ggcagcgact ttctttggaa ttccatgcct tatttttgag atcctaggct
      661 caaaaatgcc aagtggcgtc aacattaaca ttgacgccac ttttaccaca acgaaaaaca
      721 tctgttttgg ctcactccac acgcagaaat aggaaattgg atgtcaaatc agcagagctg
      781 ttcatgcttt ttgagcctaa aactgagttt gtctgcgttc tgagcactcc ttgggagtgc
      841 tggacaactc ttaacgattc ttcagttcaa gataacgttt gtaccaaata gtgatatagg
      901 ccttggaaaa tggtcctttt tcatgattaa tccaatcaac caatattttg acattttctt
      961 ttaggataaa gtccatatcg gctgaatact gcatctcttt tttatgaatc tcgtattcat
     1021 caacatcaag taatctcttt tcaccatccg caaagacttt gacatctaaa tcgtaatcaa
     1081 tgtatttaag ggcctcagtg tccatcatgt atggactagc taagttacag taataagaga
     1141 cgccattatc tcgaatcatg gctataatgt taaaccagta ttttttatga aagtaaacga
     1201 ttgcaggttc gcgggtcacc catcgtctac cgtcactttc tgtgacaaga gtgtgatcat
     1261 taactccaat gagggcattt tcagttgttt ttagtaccat agtatcgcgc caagtacggt
     1321 gcaaactacc atcatgctta taactttgaa ttgtaataaa gtcgccttct ttaggtaatt
     1381 tcatgcctta ccaactttct acaattaaac ttatcctcta tagtctatca taaatgtgtg
     1441 aaaaaatcac ttcttttgtc agtctataaa taatttctta acttgcaatt aatgtcgtcg
     1501 ctgttgtagc cttttcggta gagagcctga tagagctttt gctttaaggt ataaccatca
     1561 tatttgcgac tgagtttacg atattgtttg tctaattctt tgtcaagaag atcttctatt
     1621 tcttgattat cttggtcaaa attaaggtga ttgaggctat gtttagctag atcgtaagaa
     1681 aagcctttgg tcagtaatgc ttgggtgatt ttatctttca aggcttttgg tggcaatttt
     1741 tcttgatatt tgtcaaagag tttttggctt actttttgag cgagttggct aaagtcagtt
     1801 tgagataaga taggatcaat gtcatgactt gcaatgcctt tttgtaataa tttttgtttt
     1861 aagacttggg gacctttatc accatttaac tgattttggc gaatgtaggt atcagccaat
     1921 ttggtgtcgt ctatccattg ttcttcttgg agttgagtga tgatgtcagc aataatgtgt
     1981 tcttcaatct catgcttgcg caggtaatca gcaacttgct tgttgctgcg ttgttgaaag
     2041 gaaagaaaat aaagggctaa atttttgccg taggacagtt gggcaaaatg tttcatgtct
     2101 tcaagctgat cattgtcaag gactttatct ttactcaaca taaaccgaac aatagtatct
     2161 tctgttacat aaagggattc gtcattatcc aattcgataa ggtagaggcg ttttttcttt
     2221 tcaattttag tgattttcat actttgatta tatcaaaaat ggtaaaatag aagatgaaaa
     2281 tactgctaaa aatgtgttgc ttgtgaatgc ttttggtggt gagattagga tattagagga
     2341 agtaagggga agagatggtc gtaaaggtta aacagaaaat tcctttaaaa attaagcgaa
     2401 tgggaattaa cggtgagggg attggttttt atcagaaaac tttggtattt gtccctggtg
     2461 ctttaaaggg agaaaatatt ttttgccaaa tcacggcggt taaacggaat tttgcagagg
     2521 ccaagctgtt aacggtaaac aaggcttcta agaatcgtgt caaaccagct tgttctgttt
     2581 atgaaacttg tggtggttgc caaatcatgc atctagccta tcctaaacag ctagatttca
     2641 aagatgatgt gattaggcag gcccttaaaa agttcaaacc aactggctat gaacaatttg
     2701 agattagacc gactttagga atgaaaaagc cagatcacta tcgggccaaa ttgcagtttc
     2761 agttgcggtc atttggcggg acggtcaagg ctggtttatt ttcccaggga agtcatcgct
     2821 tggttcctat tgacaattgc ttggtacagg atcaactaac gcaagatatt atcaataaga
     2881 ttacgcagtt agtggacaag tacaagttgc ccatttacaa tgagcgcaag attgctggga
     2941 ttcggacgat aatggttcgt aaggcacagg ctagtgatca ggtacagatt attgttgtga
     3001 gtagcaaaga agttcggtta gcgaatttca ttggcgaatt gactaaggca tttcctcagg
     3061 ttaaaacggt tgccttgaat agtaatcgtt ctaaatcgag tgagatttat ggggacgaaa
     3121 cggaaattct atggggacaa gaggcgattc atgaagaagt tttagactat ggatttgcgt
     3181 tatcaccgag agctttttac caacttaatc cacagcagac agaagttttg tatggtgagg
     3241 tggtgaaggc cttggatgta gggagcaaag atcatattat tgatgcctat tgtggggttg
     3301 gctcgattgg ctttgctttt gcagggaagg tcaagtctgt gcgggggatg gatattatcc
     3361 cagaagcgat tgaagatgca caaaaaaatg ccaaagccat gggctttgac aacgcttact
     3421 atgaggcagg gaaggcagag gatattattc ctaaatggta taaacaaggt taccgcgcgg
     3481 atgccatcat tgtggaccca ccaagaactg gtttagatga taaattatta aaaacgattc
     3541 ttcattacca gcctaaacag atggtttatg tgtcttgtaa tacctccact ttggcccgcg
     3601 acttggtgca gctaacaaag gtttacgatg ttcactatat ccaaagtgta gacatgttcc
     3661 cccatacagc taggaccgag gctgtcgtga agttacaaaa acgtgtatga ttgagtggca
     3721 ataacaactc aagttttata agctcaggta atgggtttga gcaatagctt gacatagtag
     3781 ggatgtcaaa aacggtttac taagcatagt catattattg ataactctat gtttatttta
     3841 gaaacattca tggttaaaaa agtttgttag tttgtcacta agtagggtct atttgaagag
     3901 caagtacaca cagacaatat taggggaatt gattgttgaa aaatagctgt tttttgctat
     3961 aaattagaaa actgagtggt caggaggtta cattaccaga agttatttta gtatgctaat
     4021 atgaacgatg atatagagga tttatcctga tttaatcttt taagctgtaa gaaacctttg
     4081 caaaaactgt aggctgtgtg agtctttatg tgttagatat taaagataaa tgcaacactt
     4141 ttttataatg agatcaacaa ctgataaatc tgag
//
LOCUS       DATURV010000093         3978 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DATURV010000093 DATURV010000000
VERSION     DATURV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.093"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            317..580
                     /locus_tag="VCF75_001551"
     CDS             317..580
                     /locus_tag="VCF75_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEP2547095.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            585..2318
                     /gene="ptsP"
                     /locus_tag="VCF75_001552"
     CDS             585..2318
                     /gene="ptsP"
                     /locus_tag="VCF75_001552"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEP2547096.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            2503..3930
                     /locus_tag="VCF75_001553"
     CDS             2503..3930
                     /locus_tag="VCF75_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP2547097.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
BASE COUNT         1239 a          728 c          872 g         1139 t
ORIGIN      
        1 aaagcaagct ataaacttat ctaaaacaca acatattgtg ttgaaataaa aaacagaaac
       61 ctaaatattg tgtcaatctg gattcttatg gttagagctc tagcgttttg gtgatagaag
      121 ctaagcagtt ttaataaagc attaaagtct gaaaaataaa ttgtgaaaaa cgtgaaaata
      181 aaagaaaata atttcacatt ttttgtcgtt taaatattta atcttgaaaa taaaaaagca
      241 ttgtgctata atcggttgtt gtaagggtta tcatgtgatg gctcaaaaat acacaactta
      301 aaaggagaac catactatgg cttcaaaaga ctttcacatt gttgcagaaa caggtattca
      361 tgcgcgtcca gcgactttgc ttgttcaaac agctagcaaa tttgcttcag acatcactct
      421 tgactacaaa ggtaaagcag taaatctaaa atcaatcatg ggtgttatga gtcttggtgt
      481 tggtcaaggt gcagatgtta ctatctcagc tgaaggtgca gatgctgaag atgccattgc
      541 agcaattgaa gaaacaatga ctaaagaggg attggcataa gactatgaca gaaatgctta
      601 aaggaattgc agcctcagac ggcgttgctg ttgctaaagc atatctacta gttcagccgg
      661 atttgtcatt tgagactgtt acagtcgcag atacaaatgc agaagaagct cgccttgatg
      721 ttgcactcca agctgcacaa gacgagcttt ctgttatccg tgaaaatgca gtagaaagct
      781 taggtgaaga agcagcagcc gtttttgatg cccatttgat ggttcttgct gatccagaaa
      841 tgatcagcca ggttaaagaa acgattcgcg caaaacaaac gaatgcagaa acaggtctta
      901 aagaagtgac tgacatgttc atcaccatct ttgaaggcat ggaagataac ccatacatgc
      961 aagaacgcgc agcggacatc cgcgacgttg caaaacgtgt gttggctcac cttttaggtg
     1021 taaaacttcc aaatccagct acaatcaatg aagaatcaat cgttatcgca cacgatttga
     1081 caccttcaga tactgctcaa cttaacaaac aatttgtaaa agcatttgtt acaaatatcg
     1141 gtggtcgtac aagtcactca gctatcatgg cacgtacact tgagatcgct gcggtacttg
     1201 gaacaaatga tattacaaaa cgtgttaaag atggtgatgt gattgccgtt aatggtatca
     1261 ctggtgaagt gattatcgat ccaagcgaag atcaagtact tgcttttaaa gaagctggtg
     1321 cggcttatgc caaacaaaaa gcagagtggt ctctccttaa agatgcgcat actgaaacag
     1381 ctgatggcaa acactttgaa ttggctgcta atatcggtac acctaaagac gttgaaggtg
     1441 ttaatgacaa tggcgctgaa gctgttggcc tttaccgtac tgagttcttg tacatggatt
     1501 ctcaagactt cccaactgaa gacgaacaat acgaagctta caaggcagtg cttgaaggca
     1561 tgaatggcaa acctgttgtg gttcgtacga tggatattgg tggcgacaag gaacttcctt
     1621 actttgacct tccaaaagaa atgaatccat tccttggttt ccgtgctctt cgtatttcca
     1681 tctctgaaac tggggatgcc atgttccgca cacaaatgcg tgcgcttctt cgtgcctctg
     1741 ttcacggaca acttcgtatt atgttcccaa tggttgccct tcttaaagaa ttccgtgctg
     1801 caaaagcagt ctttgatgaa gaaaaagcaa acttgcttgc agaaggcgtt gcggttgctg
     1861 atgacatcca agttggtatc atgattgaga ttcctgcagc tgctatgctt gcagaccaat
     1921 ttgctaagga agttgatttc ttctcaattg gaacaaacga ccttatccaa tacactatgg
     1981 cagcagaccg tatgaacgaa caagtatcat acctttacca accatacaac ccatcaatat
     2041 tacgtttgat caacaatgtg atcaaagcag cgcacgctga aggtaaatgg gcaggtatgt
     2101 gtggtgagat ggcaggtgac caacaagctg ttccacttct tgtcggaatg ggcttggatg
     2161 agttctctat gtcagcaact tcagtacttc gtacgcgtag tttaatgaag aaacttgact
     2221 ctgctaagat ggaagaatat gcaaatcgtg cgcttacaga atgttcaaca gcagaagaag
     2281 ttcttgaact ttctaaagaa tacgtttctg aagattaacg ggttaaagag ttggtttaaa
     2341 ccagctcttt tttgctagct aaaaatacaa ttactttttg gtgtgaaatc atttgagtca
     2401 tcaaatgatt atcagaattt tcagatattt agtgtagcca aatcttgaat tatatcggaa
     2461 aagtgataga ataagattac tatcataaaa ggagataaaa cgttggcaaa acaatataaa
     2521 aatttagtga acggtgaatg gaaactatca gaaaacgaga ttaccattta cgcaccagca
     2581 acaggtgaag agttaggatc agttccagcg atgacgcagg cagaggtaga tgctgtttac
     2641 gcttcagcta aaaaggctct atcagattgg cgcgccttgt cttatgtgga acgtgcagct
     2701 taccttcata aagcggctga tattttagta cgtgatgctg aaaagatcgg cgcgattctt
     2761 tcaaaagaag tagccaaagg tcacaaggca gctgtcagtg aagttattcg taccgctgaa
     2821 atcattaatt atgcagcaga agaagggctt cgtatggaag gtgaagttct tgaaggtggt
     2881 agcttcgaag ccgcaagtaa gaagaagatt gctattgttc gtcgtgaacc agttggttta
     2941 gttcttgcca tctcaccttt taattatccc gttaacttgg caggttctaa aattgctcca
     3001 gcccttattt caggaaatgt tgttgctctt aaaccaccaa cacaaggctc tatttctggt
     3061 ttgttactag cagaagcttt tgcagaagct ggtattccag caggtgtctt taataccatt
     3121 acagggcgag gttctgttat tggggattac atcgttgagc acgaagcggt taactttatc
     3181 aactttacag gttctactcc aattggggaa ggaatcggta aattagcggg tatgcgacca
     3241 attatgcttg agcttggcgg taaggattct gctatcgttt tggaagatgc agatcttgtt
     3301 ttagcagcga aaaatattgt agccggtgct tttggttact caggacaacg ttgtacagcg
     3361 gttaaacgcg ttcttgtgat ggacaaggtg gcggatcaat tggcggctga gattaaaaca
     3421 cttgttgaaa aactaagtgt cggaatgcct gaagacgatg ctgatattac accattaatt
     3481 gatacatcag ctgctgattt tgttgaaggg ttgattaaag atgcaactga taagggagct
     3541 actgctttga cagcctttaa tcgtgaaggc aatcttattt cacccgttct ctttgatcat
     3601 gtgacaactg acatgcgttt ggcatgggaa gagccgttcg gcccagtatt accaattatt
     3661 cgtgtaacca ctgtagaaga agccatcaag atttctaatg agtctgaata tggtttgcaa
     3721 gcttctattt ttacaactaa tttcccaaaa gcttttggca ttgctgagca attagaagtt
     3781 ggaactgttc accttaacaa taaaacacaa cgtggaacag ataatttccc attcttaggc
     3841 gctaaaaaat caggtgcagg ggtacaagga gttaaatatt ctatcgaagc tatgacaact
     3901 gttaaatctg ttgtatttga tatccagtaa taaaaaaaat aaacaaaaag ttaggttaac
     3961 ttaacttttt gtttattt
//
LOCUS       DATURV010000094         3806 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DATURV010000094 DATURV010000000
VERSION     DATURV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.094"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            30..1253
                     /locus_tag="VCF75_001554"
     CDS             30..1253
                     /locus_tag="VCF75_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEP2547098.1"
                     /translation="MPSMKHILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            1312..2118
                     /locus_tag="VCF75_001555"
     CDS             1312..2118
                     /locus_tag="VCF75_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEP2547099.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIITNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            2351..3706
                     /gene="dnaA"
                     /locus_tag="VCF75_001556"
     CDS             2351..3706
                     /gene="dnaA"
                     /locus_tag="VCF75_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEP2547100.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
BASE COUNT         1355 a          652 c          641 g         1158 t
ORIGIN      
        1 ctatggacaa ataattcgga ggacaaaaca tgccaagtat gaaacatatc ttaaaatcct
       61 taagtatttt actaattgga tttttaggag gattaatagc aattattaca ttcaataatc
      121 tctacccaca ttctccttca aaaataaatt caggtaaggc aacaactagt aatatggttt
      181 ttaacaacac aaccaatact actaaagctg tcaaagctgt tcaaaatgca gttgtatcag
      241 ttattaatta tcaagataac ccttcttcat ccctttctaa cccttataca aaactctttg
      301 gagaagggcg ttcaaaagag aataaggatg ctgaattatc tatttttagt gaaggatctg
      361 gggtcattta tcgaaaagat ggcaactccg cttacgttgt tactaataac catgttatcg
      421 acggagctaa acggattgaa attcttatgg cagacggatc taaagttgtt ggtgaattag
      481 ttggagctga tacttattcg gatttagctg ttgttaagat ctcttcagat aagataaaaa
      541 cagtagctga atttgcagac tctacaaaac taaatgttgg agaagttgct attgctatcg
      601 gcagcccact aggaacacaa tacgctaatt ctgttactca aggaatcgtc tctagtttaa
      661 gtcgtactgt aactttaaaa aacgagaatg gtgagactgt ctcaacaaat gctattcaga
      721 cagatgctgc tattaaccct ggaaactctg gtggaccact aattaatatt gaggggcaag
      781 taatcggaat taattcaagt aaaatttctt ctaccccaac tggtagcaac ggtaatagtg
      841 gtgctgttga aggaattggt tttgctatcc catctactga cgttattaaa attattaaac
      901 aactagaaac taatggtgaa gttattagac ctgctcttgg aatatccatg gtcaatttaa
      961 atgacttatc cacaaatgct cttagccaaa ttaatattcc aactagtgta actggtggta
     1021 ttgtagtagc agaggttaag gaaggaatgc cggcatctgg caaacttgct cagtacgatg
     1081 tgattacaga aattgatggc aaaacagtta attcaattag tgatttacaa agtagtctat
     1141 acggacacga tattaatgat actattaaag taacttttta tagaggaact acaaagaaaa
     1201 aagcagatat caaattaaca aaaactactc aagatttgac taaaacgcag taaacaagct
     1261 acctaaatac tattacattt tatcacataa aacagtccaa aggactgttt tatgtttctt
     1321 attctatcca ggaactccct tatgacaaaa gaattattga tagacttacc aattgaagat
     1381 attattacaa atccctatca gccaaggata caatttaatc aaagagaact ccaagaccta
     1441 gcaacttcta ttaaatcaaa cggcctcatt caacctatta ttgttcgaaa atctgacatt
     1501 tttggctatg agttagttgc tggagaacga cgacttaaag cttcaaaaat ggctggtcta
     1561 aaaaaagttc cagctatcat caagaagatc tctacactcg agagcatgca acaagctata
     1621 gttgaaaatt tacaacgttc taaccttaac gctatcgaag aagctaaagc ctatcagtta
     1681 ttagttgaaa aaaaacacat gactcacgat gagattgcta aatatatggg aaaatcaaga
     1741 ccttatatta gcaatacctt acgtctgtta caactcccag caccaatcat taaagcaatt
     1801 gaagaaggaa aaattagtgc gggacacgcg cgtgctcttt taactttgag tgacgataag
     1861 caacaactgt acctcactca taaaatacaa aatgaaggcc taagtgttag gcaaattgag
     1921 caactagtta cttctactcc aagttcgaag ctatctaaaa aaactaaaaa tatttttgcc
     1981 acttctttag agaaacaatt ggctaaatca ttgggactct ctgtcaatat gaagctgaca
     2041 gcaaaccata gtgggtacct tcagatatct ttttccaatg atgatgaatt aaacagaatt
     2101 atcaacaagc taaagtagct tgttgatatt ctgttttttc ttttttagtt ttccacataa
     2161 aaaatagttg aaaacaatag cggtgtcacc ttaaaatggc ttttccacag gttgtggaga
     2221 acccaaatta acagtgttat tttattttcc acaggttgtg gaaaaactaa ctattatcca
     2281 ttgttctgtg gaaaactaga atagtttatg gtagaatagt tctagaatta tccacaagaa
     2341 ggaacctagt atgactgaaa atgaacaaat tttttggaac agggtcttgg aattagctca
     2401 gagtcaatta aaacaggcaa cttatgaatt ttttgttcat gatgcccgtc tattaaaggt
     2461 cgataagcat attgcaacta tttacttaga tcaaatgaaa gaactctttt gggaaaaaaa
     2521 tcttaaagat gttattctta ctgctggttt tgaagtttat aacgctcaaa tttctgttga
     2581 ttatgttttc gaagaagacc taatgattga gcaaaatcag accaaaatca atcaaaaacc
     2641 taagcagcaa gccttaaatt ctttgcctac tgttacttca gatttaaact cgaaatatag
     2701 ttttgaaaac tttattcaag gagatgaaaa tcgttgggct gttgctgctt caatagcagt
     2761 agctaatact cctggaacta cctataatcc tttgtttatt tggggtggcc ctgggcttgg
     2821 gaaaacccat ttattaaatg ctattggtaa ttctgtacta ttagaaaatc caaatgctcg
     2881 aattaaatat atcacagctg aaaactttat taatgagttt gttatccata ttcgccttga
     2941 taccatggat gaattgaaag aaaaatttcg taatttagat ttactcctta ttgatgatat
     3001 ccaatcttta gctaaaaaaa cgctctctgg aacacaagaa gagttcttta atacttttaa
     3061 tgcacttcat aataataaca aacaaattgt cctaacaagc gaccgtacac cagatcatct
     3121 caatgattta gaagatcgat tagttactcg ttttaaatgg ggattaacag tcaatatcac
     3181 acctcctgat tttgaaacac gagtggctat tttgacaaat aaaattcaag aatataactt
     3241 tatttttcct caagatacca ttgagtattt ggctggtcaa tttgattcta atgtcagaga
     3301 tttagaaggt gccttaaaag atattagtct ggttgctaat ttcaaacaaa ttgacacgat
     3361 tactgttgac attgctgccg aagctattcg cgccagaaag caagatggac ctaaaatgac
     3421 agttattccc atcgaagaaa ttcaagcgca agttggaaaa ttttacggtg ttaccgtcaa
     3481 agaaattaaa gctactaaac gaacacaaaa tattgtttta gcaagacaag tagctatgtt
     3541 tttagcacgt gaaatgacag ataacagtct tcctaaaatt ggaaaagaat ttggtggcag
     3601 agaccattca acagtactcc atgcctataa taaaatcaaa aacatgatca gccaggacga
     3661 aagccttagg atcgaaattg aaaccataaa aaacaaaatt aaataacatg tggaaaagaa
     3721 tatcttttat gaaatagtta tccacaagtt gtgaacaacc atttagtctt ggattctctc
     3781 gtttatttag agttatccac tatata
//
LOCUS       DATURV010000095         3782 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DATURV010000095 DATURV010000000
VERSION     DATURV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.095"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            111..1469
                     /locus_tag="VCF75_001557"
     CDS             111..1469
                     /locus_tag="VCF75_001557"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEP2547101.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDEDFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISAILAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYSNFEVRGDEFLATFDCLRG
                     DA"
     gene            1469..2551
                     /locus_tag="VCF75_001558"
     CDS             1469..2551
                     /locus_tag="VCF75_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEP2547102.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFILAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            2552..3700
                     /locus_tag="VCF75_001559"
     CDS             2552..3700
                     /locus_tag="VCF75_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEP2547103.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKTKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLI
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQAHG"
BASE COUNT         1235 a          694 c          786 g         1067 t
ORIGIN      
        1 ttggaaacgg attctaattc taaactcaag aacggtattt ttaaaatgtt gaaaaagcat
       61 ctaaataaag gtaaaataga aatcaaatag aatggatagt gtgagcttag atgaaagtga
      121 taagtaattt tcaaaacaaa aaaatattaa tattggggtt agccaaatca ggcgaagcag
      181 cagcaaaatt attgactaaa cttggtgctt tagtgactgt taatgatagt aaaccatttg
      241 accaaaatcc agcggcacaa gccttgttgg aagaggggat taaggtcatt tgtggtagcc
      301 acccagtaga attattagat gaggactttg agtacatggt taaaaaccct gggattcctt
      361 atgataatcc tatggttaaa cgcgcccttg caaaggaaat tcccatcttg actgaagtag
      421 aattggctta tttcgtatct gaagccccta ttatcgggat tacaggatca aacgggaaga
      481 caaccacaac gacaatgatt gccgatgttt tgaatgctgg cgggcaatct gcactcttat
      541 ctggaaacat tggttatcct gcttcaaaag ttgttcaaaa agcaattgct ggtgatactt
      601 tggtgatgga attgtcctct tttcaattag tgggagtgaa tgcttttcgc cctcatattg
      661 ctgtcatcac taatttaatg ccgactcatc tggactatca tggcagtttt gaggattatg
      721 ttgctgctaa atggatgatt caagctcaga tgacagaatc agactacctt attttaaatg
      781 ctaatcaaga gatttcagca attctagcta agaccaccaa agcaacagtg attccttttt
      841 caactcaaaa agtggttgat ggagcttatc tgaaggatgg aatactctat tttaaagaac
      901 aagcgattat agctgcaact gacttaggtg tcccaggtag ccacaacatt gaaaatgccc
      961 tagcaactat tgcagttgcc aagttatctg gtattgctga tgatattatt gcccagtgcc
     1021 tttcacattt tggaggcgtt aaacatcgtt tgcaacgggt tggtcaaatt aaagatatta
     1081 ccttctacaa tgacagtaag tcaaccaata ttttagccac tcaaaaagct ttatcaggtt
     1141 ttgataacag tcgcttgatt ttgattgctg gcggtctaga tcgtggcaat gaatttgacg
     1201 atttggtgcc agacctttta ggacttaagc agatgattat tttgggagaa tccgcagagc
     1261 gtatgaagcg agctgctaac aaagcagagg tctcttatct tgaagctaga aatgtggcag
     1321 aagcaacaga gcttgctttt aagctggccc aaacaggcga tactatcttg cttagcccag
     1381 ccaatgctag ctgggatatg tattctaatt ttgaggttcg tggggatgaa tttttggcaa
     1441 cctttgattg tttaagagga gatgcctaat gcctaagaag attttattta caggtggtgg
     1501 aactgtaggt catgtcacct tgaacctcat tctcatacca aaatttatca aggacggttg
     1561 ggaagtacat tatattggtg ataaaaatgg cattgaacat acagaaattg aaaagtcagg
     1621 ccttgacgtg acctttcatg ctatcgcgac aggcaagctt agacgctatt tttcatggca
     1681 aaatctagct gatgttttta aggttgcact tggcctccta cagtctctct ttattcttgc
     1741 caagcttcgc cctcaagccc ttttttccaa aggtggtttt gtctcagtac cgccagttgt
     1801 ggctgctaaa ttgcttggta aaccagtctt tattcatgaa tcagatcggt caatgggact
     1861 agcaaacaag attgcctaca aatttgcaac taccatgtat accacttttg agcaggaaga
     1921 ccagttgtct aaagttaaac accttggagc ggtgacaaag gttttcaaag atgccaacca
     1981 aatgcctgaa tcaactcagt tagaggcggt gaaagagtat tttagtagag acctaaaaac
     2041 cctcttgttt attggtggtt cggcaggggc gcatgtgttt aatcagttta ttagtgatca
     2101 tccagaattg aagcaacgtt ataatatcat caatattaca ggagaccctc accttaatga
     2161 attgagttct catctgtatc gagtagatta tgttaccgat ctctaccaac ctttgatggc
     2221 gatggctgac cttgtagtga caagaggggg ctctaataca ctttttgagc tactggcaat
     2281 ggctaagcta cacctcatcg ttcctcttgg taaagaagct agccgtggcg atcagttaga
     2341 aaatgccact tattttgaga agaggggcta cgctaaacaa ttacaggaac ctgatttaac
     2401 tttgcataat tttgatcagg caatggctga tttgtttgaa catcaggctg attatgaggc
     2461 tactatgttg gcaactaagg agattcagtc accggacttc ttttatgacc ttttgagagc
     2521 tgatattagc tccgcgatta aggagaagta aatggcaaaa gataaagaga aacaaagtga
     2581 tgacaagctc gttttgacag agtggcaaaa gcgtaacatt gaatttttaa agaaaaagaa
     2641 gcagcaagct gaggaagaaa aaaaactcaa agagaaatta ttgagtgata aaaaagcgca
     2701 gcagcaagct caaaatgctt ctgaagcagt tgagcttaaa actgatgaga aaactgatag
     2761 tcaggaaatt gagtcagaaa cgacgtcaaa acctaaaaaa accaaaaaag ttagacaacc
     2821 caaggaaaaa agcgcgacac aaatcgcttt tcaaaaatcc ttgcctgttc ttttgggggc
     2881 gctcttactc atggcggtgt ctatttttat gatcactcct tatagcaaaa agaaagagtt
     2941 ttctgtaaga ggaaaccatc aaacgaacct tgacgaatta atcaaagcta gcaaagtcaa
     3001 agcatctgac tattggttaa cgttgttaat ttcgcctggt cagtatgaac gaccgattct
     3061 tcgtactatt ccatgggtga aatctgtaca tctctcttac caatttccta atcactttct
     3121 atttaacgtt attgaatttg aaatcatcgc ttatgcacaa gtcgaaaacg gttttcagcc
     3181 tattttggag aatggaaaac gtgtggacaa ggtcagggca tcagaactac cgaaatcttt
     3241 cttgattctt aatttaaaag atgagaaagc gatccaacag ttagttaagc aattaacgac
     3301 attacctaaa aaattagtca agaatatcaa gtcagtgtct cttgcaaatt ccaaaacgac
     3361 agcggattta ctacttattg aaatgtatga cggtaatgta gttagagtac cgcagtcaca
     3421 actcacattg aaacttccct attatcaaaa attgaaaaaa aaccttgaaa atgatagtat
     3481 agtggatatg gaagttggaa tttatactac aacacaggag attgaaaatc aacctgaagt
     3541 tcctcttacg ccagaacaaa acgcagctga taaagaagga gataagcctg gtgaacatca
     3601 ggaacagaca gacaatgatt cagaaacgcc agcaaatcag agtagtcctc agcaagcacc
     3661 accatcccca gaaacggtcc tcgaacaggc ccatggctag ctaatatcta agttgaaaaa
     3721 gcaatgaaaa cgttagaaat tcaacgattc taacccataa tgaattgcct aaaaaaatta
     3781 ag
//
LOCUS       DATURV010000096         3603 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DATURV010000096 DATURV010000000
VERSION     DATURV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.096"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..660)
                     /locus_tag="VCF75_001560"
     CDS             complement(<1..660)
                     /locus_tag="VCF75_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2547104.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVGY
                     IL"
     gene            complement(1006..3543)
                     /locus_tag="VCF75_001561"
     CDS             complement(1006..3543)
                     /locus_tag="VCF75_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEP2547105.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
BASE COUNT         1177 a          733 c          537 g         1156 t
ORIGIN      
        1 tagaatatac cctacgcttc ttaaagtttg aagcctgtta acaaaacggg taccttttaa
       61 ttttttcctt attttagaaa tgtaaacttc aacaaccgaa atcgtagtat cactatcgaa
      121 tccccataaa cgatcaaaaa tttgtgtctt tggtaaaata acgttttgat tttgcaaaag
      181 atacaccaac aaatcaaatt ctttgccaag taattcaact actttccctt caaccttcac
      241 ctcttttcga gctaagtcga ccactaaatt gccaaaacta atatttttat ctgcaaattt
      301 ccctgttctt tttaacagtg cttgaattcg catttttaac tcttctaaat agaacggctt
      361 tgtcagataa tcatcagcac ctaattcaaa tccgtgacct ttatcatcta aaccctcttt
      421 agctgtcatg atcaacacag gaattttaat atctttttct cgcaattctt ttaagacttg
      481 aaagccattt ttctccggca acataaggtc taacaaaatc aaatcataaa taccactttc
      541 tgcttcgtat aaaccttcat cgccatcaaa aacttgcatg acgtctgcaa aatcatctaa
      601 aaaatcaaaa atggaatttg ataagctcaa gtcatcttct actaataata tctttatcat
      661 gtctctacct caattgttct taaataaagt aactatcact tcatttaact tagtctcatt
      721 tctgataata atttctaacg agactatact acaaaaagct taaggtttac taaaatatta
      781 atactccttt aaggcattta aagcataatg attaaatcat ttataattgt tcttattcac
      841 cacctggtaa aaaaagtatt aagaggctaa tagccttcgc tttaaagtgc aatctccttt
      901 tgaattaaac tatagataat cacttagtta tagacggaac taagaaagga agctagctca
      961 ctacaaaaaa gagctggaaa gtcccccaac tcttttgctt atggtctagt aatgactgag
     1021 agctgcaata actgcttctt tttctttagt gatcaaagca acacgtgctt caatttcatt
     1081 aattcccatg gtaatattac ggctaatagc catatcatct agcttaggtt caaagaaaga
     1141 tttatattct gctagatgtt ttggtgtttt aaagttactt gctggataaa taacaaattt
     1201 atcaaagctc atatctccac caagtgcaga tttaatccaa tcccaatttt ctctagccca
     1261 ttcccaaatg atatcttgag taaagctttg tccaagcaaa gcattatacc aataagataa
     1321 atcctgaggt ttaataatat ctttatcttt taaactaaca agaatacgtc taatagtagt
     1381 tggttgtttt gtttgactaa aagcaaccgt taaatcactg cgtaaattat tatcagtagt
     1441 tgctacataa gtctcaaaat aaatatcaac caagctatct gtttcaaaat atttcatttg
     1501 gttggctaaa accaagcgac gaacagctgc tggaatactt gcaatattgt tttgatatgc
     1561 ttcaaaaata gttctcaatc cgtcaataat cgtctgattt tctgctagcc acaagcggcc
     1621 caaggcaacc tgacgtacca tctcatcttc atctgattcg tcagcttttt tctcaaaacc
     1681 atgctgatta aagtcttctt gatagatggt ggttaccaag cgattaaagg atttttctgc
     1741 taagcttcct tcatcaatga agcgtttcaa accagagacc acttgctgaa ccgcttctgc
     1801 taccatataa gacttagaat cgtccaattg agcaattaag tcaaccaatt cagcatagga
     1861 aatcaagcca ctatctgcca ataaacgacg ttcttgaata acttgtaaag cagaagtatt
     1921 atcaagttgg gcaagttcag tgactaaggc atctaataat gatccttggt aatctgtaat
     1981 gtaatgagct gtattatcaa tattgaaacg tagagctcct tcattttctg ctgccaattg
     2041 actaaagttt ggaatgacca tttctgcctc tgtcaaagtt tcaggaaggc catgccaatt
     2101 agtgtttaaa ggaatcggcc aaaggcgacc tttttcttca ccttttccaa taaaaaattg
     2161 tttttgactc aaaatcaatt gatcgttttc aaggcgagct gttaaaactg gataacctgg
     2221 ttgttcaagc caagcatcca taaaagcagc tacgtctttg cctgaagtat ctgataaaat
     2281 attccataaa tcacgcccga cagtatttcg atattgatat ttttcaaagt agtgatgtaa
     2341 gccaatggca aaatcacgat caccaataaa acggcgtaac atatgcatca aacgactacc
     2401 ttttgcatag acaatagcag gatcaaacaa tgtatttatc tcatctggat gattgacttc
     2461 aacatgcact gattgaacac catcagtagc atcacgtttt aaagcaagag gaataccgcc
     2521 tgtttggaaa tcttctatta ttttccaaga cggctcaata gcctcgatag agacatattc
     2581 catcatgttg gcaaaactct catttaacca cagatcgtcc caccatttca ttgtaaccaa
     2641 gtttccaaac cattggtgtg ctatctcgtg ggcgattaca agtgccactt gttgtcggct
     2701 ttgaacagtc gaattttcat ctactaataa ataaatttca cgataagtga ttaaacccca
     2761 attttccata gcaccagatg aaaaatctgg aagcgcaata ttaagggatt gcgggattgg
     2821 gtaatgtact ccaaaatagt cttcataaaa gttaataaca cgaactgcaa tatccaatga
     2881 aaaatcaagc gagctaagag gatgcgctgt tgtcgcatac acaccaacag ttgttccttt
     2941 tttgctttct acagtctttc catgaagttc tcctagcgca aacgctagca agtaagatga
     3001 cattcttggt gtcgtatcaa atgtccaaag accagtctcc tcgcgacggt caatattgac
     3061 ttctggcata ttcgataacg ctatctcacc aatttcttga tcaaaggtca agcttaaatc
     3121 aaaagtagct tttgcttgtg gttcatcgat acatgggaaa gcttcacgtg caaaatggct
     3181 ttcaaactga gttgaaatga cttctttttt ctcaccattt ttagtgtaat atgaagggta
     3241 aatacctgtc atattatctg taatgctacc tgaaaattca atgacaagcg tcatcattcc
     3301 tgtttcaggt aattcaacac gcacaacttc attgtcgtta tccacttgat aaataacagc
     3361 ttcattatcg agcaaaatag acttaatgtc caaatctttt tggtggaaag ccacatgatt
     3421 atctaaagct tcaccattaa tcgcaacatt ccccgtaaat gttttagtct gacgattaat
     3481 atctaaaaag atgttataat tttcaggcac aaatgtttcg atgagatgtt ctactgtctt
     3541 catataactc cttaattcta ataacaaatc ataatagatc aacagatgat acctaagtat
     3601 cat
//
LOCUS       DATURV010000097         3534 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DATURV010000097 DATURV010000000
VERSION     DATURV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.097"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            158..442
                     /locus_tag="VCF75_001562"
     CDS             158..442
                     /locus_tag="VCF75_001562"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEP2547106.1"
                     /translation="MRLEEIRQEINSIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QVRENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRKYQTQHLTGGDTND"
     gene            435..1697
                     /locus_tag="VCF75_001563"
     CDS             435..1697
                     /locus_tag="VCF75_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEP2547107.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGVAVGVIDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILLSTWVTHLFGASAGREGVAVQIGATISHYCRRFVTSQDAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFACLLGWFKPYLSQKLPNPYIRIAFIGVLLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGII
                     LAPYLGLPVLLVAALGYVTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            1812..2159
                     /gene="rplS"
                     /locus_tag="VCF75_001564"
     CDS             1812..2159
                     /gene="rplS"
                     /locus_tag="VCF75_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEP2547108.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            2407..2478
                     /locus_tag="VCF75_001565"
     tRNA            2407..2478
                     /locus_tag="VCF75_001565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2440..2442,aa:Arg,seq:cct)
     gene            complement(2523..3512)
                     /locus_tag="VCF75_001566"
     CDS             complement(2523..3512)
                     /locus_tag="VCF75_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP2547109.1"
                     /translation="MTLDKNSNQAKNKASIIIKEKIEDRLAIRDHSEMTYGELKREYL
                     NQWIPTVKDSTKRGYLVSDGHIATVLPDDTIISKLTKRDIRQFIDNLLKNHSYHVTHK
                     CRKRLHAILSYAIQMDYLTSNPTDNVLVPKPKEEYMPEKVLYLTSNEVYDLCGRMIAN
                     NEQTLADVVLFMFLTGMRYGELACLTYDKIDFENKEILINATYDFYTKEITTTKTKKS
                     TRKISVSDNILEIINRQNKVSEFIFPNSKGLPILNAYINKRLKIYGNYHTHLFRHSHI
                     SFLAEKGIPLKAIMDRVGHSDPNTTLSIYSHTTVNMKALINNQTAPFMPLKII"
BASE COUNT          997 a          630 c          710 g         1197 t
ORIGIN      
        1 gtcaggggct tccttggcat ttttagcttt ttaacctaaa ataaagggaa tgtcacttca
       61 gggtaggaat tgaaaaaaca atcagcttca ccttgcttct gatcctctta ttaactataa
      121 taaaaagata atctaataca tctagaaaag gaaagttatg cgtttagaag agatacgaca
      181 agaaatcaat agtattgatc accacttggt tgccttatta gaaaagcgga tggcacttgt
      241 agagcaagta acagcttata aactagccaa tcacttgcct gtcttagatc aggtgcggga
      301 aaatcagatc ctagataggg tttcttatct cgttaaagac caagcatttg agccagctat
      361 ccatgagacg tttaaaacca ttatgtctct ttcacggaag tatcaaacgc aacacttaac
      421 aggtggtgac actaatgatt aaatcaattg caacactgcc taaaccctcc ttaaaagaag
      481 cgttaggact aataggcttg gcgcttccca taggggtcgc ggttggagtg attgatgtga
      541 tttttggtaa agggcttttg tttctttccg agtatcgcga tcaccacctt ttcttgcttc
      601 ttccttttct tgccttagca ggccttgtta tcgtttttct ttatgataag ttaggcaaag
      661 aggttcgtca agggatggga ttggtttttc aggtagggca tggtcaaaaa gatcagattc
      721 ctcctattct cattcctctt attttgttat cgacttgggt aacccactta tttggagcga
      781 gtgcgggtcg agaaggggta gccgttcaaa taggagctac catctctcat tattgtcgga
      841 gatttgtgac tagtcaagat gccgcacgac accttttgat tatggggatg gcagccggtt
      901 ttgcaggact ttttcaaacg ccaattgctg ctgtcgtatt tgcccttgag gttttgttgg
      961 taggaacgct tcgctactgc gctttattgc cttccttagt tgctgcttgc gtggcttctt
     1021 ggacctctca ttctttggga ttagagaaat ttacgattgt tcttgaagaa acattgacca
     1081 taactccctt aaccttagtt aaattaattg ggttaggtct tatttttggt ctggtgggaa
     1141 atagttttgc ctgtttactt ggctggttta aaccttacct gagtcaaaag ctaccaaatc
     1201 cttatatcag gattgctttc attggtgttt tgttatctat ctgtttgatg attggtcacg
     1261 ttggaagata tagtggtcta gggactcatt tgattgcggc agctttttct ggccaaacta
     1321 tcctaactta cgactggctt ttaaaaatga tcgtgacggt gattagctta tctgcaggct
     1381 ttcaaggtgg tgaagtgaca cctctatttg ctattggagc ttctttaggc atcattttag
     1441 ccccttactt aggtcttcct gtactgttag ttgctgcact tggttatgtc acggtatttg
     1501 gaagtgcaac aaataccttt tgggccccta tctttattgg gatagaggtc tttggtccag
     1561 agaatgccct agcatatttt gtcacctcag ctgcagccta tatggttagt cacagacatt
     1621 ccatttacag ttatcaaaaa gtagtcatga tcaatttaaa agacatgacg gtaaatcaga
     1681 tggatttaaa aaaatagctt tacaataaag aagctttttg ttagaataaa tctcgtgtgt
     1741 aatggacaca cgaaaattac ggctaatccg ctaagacaag tacttaagat tagtaagata
     1801 ggagaataaa aatgaatcca ttaatccaaa gtttgacaga aggtcaactt cgctctgata
     1861 tccctaactt ccgtcctggt gacactgttc gcgttcacgc aaaagttgtc gaaggaactc
     1921 gcgaacgtat ccagatcttt gaaggtgttg ttatttcacg taaaggtcaa gggatctcag
     1981 aaatgtacac tgttcgtaaa atctcaggtg gtatcggggt agagcgtaca ttcccaatcc
     2041 acacaccacg tgttgacaaa atcgaagtta tccgccacgg taaagtccgt cgtgcgaaac
     2101 tttactactt gcgtgcatta caaggtaaag ctgctcgtat caaagaaatt cgtcgttagt
     2161 ttgaaccgaa acgtaacgta atagaataaa aaagactaga gtctctagtc tttttatgtc
     2221 gtcgtgacaa tgtgacaatt ggtgattaac gctatgctcg taggacactt tgaaagttcc
     2281 ttagcagtta taattagtat attggaataa caaaatgaag caaaataaac tttcagttat
     2341 ctgatatttc atcttgcatt accttatttt ctaggttaaa atagaagaca aggtgaatat
     2401 aagatagacc ccttagttca atggatataa caactccctc ctaaggagta gttgctggtt
     2461 cgattccggc aggggtcatt attgttaact taaaaagcct ttatatcagg ctttttttat
     2521 tactaaatta tttttagggg cataaaaggg gcagtttgat tattaatcaa tgctttcata
     2581 tttacagttg tatgactgta aattgataat gttgtgttgg ggtctgaatg gccaactctg
     2641 tccataatcg cttttagtgg tattcctttt tcggctaaaa acgaaatgtg tgagtgtctg
     2701 aataagtgag tatgatagtt accataaatt ttcagacgct tattgatgta tgcgtttaaa
     2761 attggtaaac cttttgaatt tggaaaaata aattcactca ctttattttg tcgattgatg
     2821 atttctaaaa tgttatcaga tacggaaatt ttgcgagttg attttttagt cttagtagtc
     2881 gtaatttctt tagtgtaaaa atcgtaagtt gcgttaatca gaatttcttt attttcaaaa
     2941 tcaatcttat cataagtcag acaagcaagc tcaccatatc tcatacccgt taaaaacatg
     3001 aataaaacga catctgcgag cgtttgttca ttgttggcta tcattctacc acagaggtca
     3061 taaacttcat tagatgttaa ataaagtacc ttttcaggca tatattcctc ttttggctta
     3121 ggtactaaaa cattatctgt cggattgctt gttaaatagt ccatttggat tgcgtaagac
     3181 aaaatagcgt gtaatctttt tctgcattta tgtgtaacat ggtacgaatg gtttttcaat
     3241 aagttgtcaa taaattgtct aatgtcgcgt ttagtcaact tgctgataat tgtatcatct
     3301 ggcaacacgg ttgcgatatg accgtctgag actaaataac cacgcttggt ggagtctttg
     3361 accgttggaa tccattgatt taaatattcg cgctttaatt caccataggt catctcagaa
     3421 tgatcgcgaa tagcgagtct atcttcaatc ttttctttga taataatact tgctttattc
     3481 ttggcttgat tactattctt atcaagtgtc acagatactt ttttatattt tccg
//
LOCUS       DATURV010000098         3511 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DATURV010000098 DATURV010000000
VERSION     DATURV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.098"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..2279
                     /locus_tag="VCF75_001567"
     CDS             <1..2279
                     /locus_tag="VCF75_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEP2547110.1"
                     /translation="YYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLNDKRFDIWS
                     LTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLCYQGNTFSVALILAVV
                     PISDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVLNALKHLKS
                     NKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAITLVALLAY
                     VITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVTSFVKKSVI
                     VEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIPFSYEKEGK
                     ILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNYSQETIASI
                     AQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPNIKAIKNST
                     TAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHTISEFYQGK
                     NRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLTSDKTVYNNILS
                     LINPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARLLSFTDKET
                     RAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNGTNEKKNHP
                     LINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQNDLKNIQYDV
                     TIGKGYLLKHKTFFKISR"
     gene            2458..3153
                     /locus_tag="VCF75_001568"
     CDS             2458..3153
                     /locus_tag="VCF75_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP2547111.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            3155..3496
                     /locus_tag="VCF75_001569"
     CDS             3155..3496
                     /locus_tag="VCF75_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEP2547112.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFTWLPNILNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSLTNNISLQNDKVKRLTQE
                     VALMKKEKTND"
BASE COUNT         1163 a          518 c          620 g         1210 t
ORIGIN      
        1 cttattattt gagtcgccaa gttagtcgac agctgttttt gaaaactagt tttattgtga
       61 tatcttatct aattgtcagc tattgggtac aaataacaca gcacctgaat gataaacggt
      121 ttgatatctg gtcattaact aaaaatcaat tttatcaatt tcaagctcta ccttctttac
      181 tcattatttt agtgatggcc actttaataa aaatattggt agcatatttt gcaatagaaa
      241 aagatagatt tgggctatta tgctatcaag gtaatacttt ttctgtagct ctgattttag
      301 cagttgtgcc aattagtgac atacatctgt taaaactaat aagttctcga ttttctgaat
      361 tagtaagagc aggtaatagc caaattgcac tgttaaaaat aagtggactg ttgatagttt
      421 tacttgtcat atttgcaaca atcatatacg tggttttaaa tgctctaaaa caccttaagt
      481 caaataaacc ttcattttca gtagcagcta ctactagttt gtttttagca ttagttttta
      541 actatacgtt ccagtatgga gtaaaaggtg atgaagcatt gctaggatat tatgttttcc
      601 ctggagctac tctttttcag atagtagcta ttacactagt tgctctttta gcatacgtga
      661 taacgaatag atattggcca actaccttct ttttgcttat tctgggaaca attatttctg
      721 ttgttaatga tttaaaagaa tcaatgagaa gcgagccgtt attagtaact gattttgttt
      781 ggttacaaga attaggttta gtgacaagct ttgttaaaaa atcggtgatt gtagaaatgg
      841 ttgtaggact tgctatttgt attgtggtgg cttggtatct acatggccga gttttagcag
      901 ggaaattatt tatgagccct gtcaaacggg caagtgctgt attaggttta tttattgtat
      961 cttgtagtat gttaatacca ttttcttatg aaaaagaagg taaaatatta tctggtcttc
     1021 cgattatttc ggctttaaat aatgataatg atataaactg gttaggtttt tcaacaaatg
     1081 ctaggtacaa atctttagca tatgtttgga caagacaggt gaccaagaaa ataatggaaa
     1141 aaccgacaaa ttatagccaa gaaacaatag cgagtatcgc tcagaagtac caaaaattag
     1201 cagaagatat taataaagac agaaaaaata atattgctga ccaaacggtt atttatcttt
     1261 taagtgaaag tttgtcagat cctgatagag tatcaaatgt tactgttagc cacgatgttt
     1321 tacctaatat caaggcaatc aaaaatagca caactgcggg actcatgcag tcagactcct
     1381 acgggggtgg aactgctaac atggagtttc aaacgttaac aagcttacct ttttataatt
     1441 tttcttcttc agtatctgtt ctttattcag aagtctttcc taaaatggcc aaacctcata
     1501 cgattagtga gttttaccaa ggaaaaaatc gtattgcgat gcatcctgct agtgctaaca
     1561 attttaatag aaaaacagtt tatagtaatt taggtttttc caaattctta gctctatcgg
     1621 gttctaagga taagtttaag aacattgaaa atgtcggttt attgactagc gataaaactg
     1681 tctataataa tattttatct ttaattaatc ctagtgaaag ccaatttttc tcagttatta
     1741 caatgcaaaa tcatattcct tggtcatccg attatcctga agaaattgtt gctgaaggaa
     1801 aaaatttcac ggaagaagaa aatcacaacc taacaagtta tgctcggtta ttatcgttta
     1861 ctgataagga aacaagagca tttttagaaa aattaacaca aattaacaag cctatcacag
     1921 tggtgtttta cggagatcat ttacccggtt tatatcctga tagtgctttt aacaagcata
     1981 ttgaaaataa ataccttact gattatttta tttggagtaa tggtactaac gagaaaaaaa
     2041 atcatccgct tatcaactca agtgatttta ctgcagcttt atttgagcat actgattcaa
     2101 aagtatcacc ttactatgct ttgttaacag aggtactgaa taaagctagt gtcgataaat
     2161 caccagatag tcctgaagtt aaagctattc agaatgattt aaaaaatatc caatacgatg
     2221 tgactatagg aaaaggttac cttttgaaac acaaaacttt ttttaagata tcacgttaag
     2281 aaaaatgcta ggctgtccga gtatagacga gcatcaaggt ttggtaattt gctcaaatga
     2341 ttgtcaacag agtgtctagg accaggttta aaagattata attcttaagg tcttattcgt
     2401 ttataaaaaa tgaatactct tttaaaatct taattgaaat gaataggaga agttttcgtg
     2461 aaaaagttaa tcattattcc tgcttacaat gaaagcagta atattgtcaa tactatacgt
     2521 actattgaat cagatgcccc ggattttgac tatatcatta ttgatgattg ctcaacggat
     2581 aatacgttag caatatgtca aaaacagggg ttcaatgtta tttctttgcc cattaacctg
     2641 ggaattggcg gtgcggtgca aactggctat cgttatgcac aaagatgtgg atatgacgtt
     2701 gcagttcaag tagatggaga tggtcagcac aatccatgct atttggaaaa aatggttgag
     2761 gtattagttc aatcttcagt aaatatggta attggatcac gatttatcac aaaagaaggg
     2821 tttcagtcat catttgctcg acgtatcggt ataaagtatt ttacttggct tattgcgcta
     2881 ctaacaggaa aaaaaataac agatgcaacg tcaggtctaa ggttaattga ccgttcattg
     2941 attgaacgtt ttgctaatca ttatccagat gattatcctg aacctgaaac agttgttgat
     3001 gtattggtta gtcattttaa agtgaaagaa attcctgttg taatgaatga gcgacaaggc
     3061 ggtgtgtcat ctatctcgtt gacgaaatcg gtctattata tgattaaagt gactttagct
     3121 attttagttg ttaggttgaa aggaaatcgt taaaatgacg gtaacagcca gtttatttat
     3181 aagttttatt gtattaacct ttgttttctt tttgattaac ttaatcaaaa aagacaaact
     3241 agcgattaag tactctttat tgtggtttat tttagcttta ttaattttac tatttacgtg
     3301 gttacctaac atattaaata aaatgtctca cttcctgggt attcattcac caaccaatat
     3361 gttattcttt ttaggttttt gtttgagttt agctattatt ttttcactga ctaataatat
     3421 ttcacttcaa aatgataaag taaaacgtct gacccaagaa gtggccctta tgaaaaaaga
     3481 gaaaaccaat gattaatcct tcaaaacaaa a
//
LOCUS       DATURV010000099         3287 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DATURV010000099 DATURV010000000
VERSION     DATURV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.099"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            68..1057
                     /locus_tag="VCF75_001570"
     CDS             68..1057
                     /locus_tag="VCF75_001570"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP2547113.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILAKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            complement(1166..1957)
                     /locus_tag="VCF75_001571"
     CDS             complement(1166..1957)
                     /locus_tag="VCF75_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEP2547114.1"
                     /translation="MTYTSLKSPEKTDYLYDLNIAHLYGNLMNTYGDNGNVLMLKYVA
                     EKLGARVTVDIVSLNDAFDQDYYDLVFFGGGQDYEQTIVAKDLPSKKAALADYIAKNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(1957..3276)
                     /locus_tag="VCF75_001572"
     CDS             complement(1957..3276)
                     /locus_tag="VCF75_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEP2547115.1"
                     /translation="MIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDLSKDYDIVV
                     VTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKSERQIAVLE
                     IDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPKATILANGD
                     SPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNTYANLGDFV
                     CLNCQFQRPTLDYQLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALAAVSVAEFF
                     GVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQLADYPFSL
                     SVLLNANYADGIDTSWIWDANFELITHMPITEINAGGVRHSEIARRLRVTGFDDTKIK
                     QAEKLEQIMEAIEKQESKHAYILATYTAMLEFRSLLADRHVVEKEMN"
BASE COUNT         1024 a          598 c          636 g         1029 t
ORIGIN      
        1 tgtcttttat atttgttttc attatactag agaggtagat tacatttttg aaaggatttt
       61 ttctcctatg aaatacatcg ttaacaaaag tcacaatcct gcctttaaca tcgctttgga
      121 agcctatgct tttagagaac tagtggaaga agacgaattg tttatccttt ggatcaatga
      181 gccagctatt attattggca aacatcaaaa taccattcaa gaaatcaata aagaatacat
      241 tgatgaacat ggtattcatg ttgtgcgacg tttatctggt ggtggtgcgg tttatcatga
      301 tttgaataac ttgaactaca ctatcatttc aaataaaaca gcggaaggcg catttgattt
      361 caaaaccttc tcacaacctg tgattgcgac tttagctgat ttgggtgtaa cagccaactt
      421 tacaggccgt aatgatattg aaattgatgg taaaaaaatc tgcggtaatg ctcaagctta
      481 ctacaaaggt cgcatgatgc accatggttg ccttttgttt gatgtggata tgaccgttct
      541 tggcgatgct cttaaggtaa gtaaggacaa gattgagtct aaaggagtga agtctgttcg
      601 tgcccgtgtg accaatattt taaatgaatt accagaaaaa attaccgttg aggaattttc
      661 agataaaatc ttggccaaga tgaaagaaac gtacccagac atgacagaat atgtcttgtc
      721 agaggatgag ttggctaaga ttgaacaatc tgccaaagag caatttggta gctgggattg
      781 gacctacggc aaagcgccag aatatacgat tgaacgcaat gttcgttacc ctgcaggtaa
      841 aatttcaacc tttgcaaatg ttgaaaattc tatcattaaa aaccttaaaa tctatggtga
      901 tttctttgga atcaaagatg tgcaagacat tgagaactta ttaattggtt gtaaatatga
      961 gtaccgtgat gtttttgaac gtttaaaaac gattgatacg acccagtact tctcacgcat
     1021 gaccgttgaa gaagttgcta aagctattgt ttcttagtgg ttttaatcac ttaagaagct
     1081 ctgtcagaag aaaaaactat aaaactccta agcgaactat cgtgtcctac ttaggagttt
     1141 ttgtttgatg acatgaaaga agaacttaaa ctttattaaa ggaagctttg cttttaaggt
     1201 cggcatattc ttcagtgatt tcttgtttca gtatatcatc atagctaggt agtgaaatag
     1261 ctgatccata ctttttttta agagctgttg ttactaagcg gtaagcaaga ttaacattgc
     1321 gtgataagat aggaccgtgg aaataactac catagacgtt tttatagtga acaccttcag
     1381 tttggtcttc tttattatta ccgttgccat aaaccactcg tccaagtggc ttttcgtcac
     1441 cagataagaa ggtacgtcct tgatgatttt caaagccata ataggtttcg ttaaattcgt
     1501 cattatgaat tttaatatct ccaataaagc gattttggtg ctgattaagg gtgtagtgtc
     1561 ccataattcc aagtccatca attttaaccc cattagcttg gacatagtat tgccctagta
     1621 attggaagcc accacagatg gcaagcacca ctttattctt agcgatataa tccgctaaag
     1681 cagctttttt gctaggcaaa tccttagcaa cgatggtttg ctcatagtct tgtccaccac
     1741 caaaaaaaac aaggtcgtag taatcttggt caaaggcatc attgagggaa acaatatcaa
     1801 cggtaactct agctccaagt ttttcagcga catacttgag catcaagaca ttcccattgt
     1861 caccgtaggt attcatcagg tttccgtaga gatgagcaat gtttaggtca tagaggtagt
     1921 cagtcttttc tggagatttt agtgaagtgt aagtcattag ttcatctcct tttcaacaac
     1981 atggcggtcg gctagcaagc tacggaattc taacattgca gtataggttg ctaaaatata
     2041 agcatgtttg ctttcttgtt tttcaatagc ttccatgatt tgctcgagct tttcagcttg
     2101 ttttatctta gtgtcgtcaa atcctgtgac tctaagtctt ctggcaattt cagagtgacg
     2161 gacaccacca gcattaatct cagtaattgg catgtgtgta atgagttcga aattagcatc
     2221 ccaaatccag ctggtatcaa tgccatcagc gtaattggca tttaaaagca cagataagct
     2281 aaatgggtaa tcagctagct gaatcatttc aagcgcttga ctggctccaa caggattctt
     2341 gataaggata agggtgcatg atttatcacc aatagtaaaa gtttcttgtc ttccaaaaac
     2401 cgccttactt ttgttaaagc ccgccttgat tttctcggga gaaacaccga aaaattcagc
     2461 aacagagacg gcagcaagcg cattgtaaat attataaaga ccaccaacat ttatcttgta
     2521 gttttgtcca tcaataacaa attcagaact ttggtgagta atagcggtga gctcagttaa
     2581 ttgataatct aacgttggtc tttggaactg acagtttaag caaacaaagt ctcctagatt
     2641 cgcataagta ttgagacgat actgaagaat atgttcgcat tttggacata aaatcccttc
     2701 tgtgttgtaa tgagctagct gcggagcatg cttagcagta tcaaaaccat aatactgaac
     2761 tggattaact atgtccttag aagagaaaat aggactatct ccattggcta gaatagttgc
     2821 ttttggagca tttcgagcac catctacaat catctgataa gtagtataaa tctctccgta
     2881 gcggtccatc tgatccctaa aaatattagt ataaacaaaa agactaggtt ttaagtaagt
     2941 ggtaattcta ggaagactag cctcgtcaat ttctaaaaca gcaatttgcc tttcagattt
     3001 ccctttttta gcagctaaaa aagtagaagt aattccagta atcatattgg caccacttgg
     3061 atttgtgatt atctcgccaa atgcctcttt caaaataccg acagtaagag cagttgttaa
     3121 cgttttacca ttagtaccag tgacgaccac aatatcatag tctttagaca aatcctttaa
     3181 aatatcttta ttacaagcta gagccaatcg accaggataa gtagatcctc gccctagttt
     3241 tgttaaaatg gattgagcag ctttgccagc aataatgccg agtaatg
//
LOCUS       DATURV010000100         3249 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DATURV010000100 DATURV010000000
VERSION     DATURV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.100"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(274..1047)
                     /locus_tag="VCF75_001573"
     CDS             complement(274..1047)
                     /locus_tag="VCF75_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2547116.1"
                     /translation="MSGSLGNKKIMASNIRRHLDKKNINPKEFSKVMGFKYSTVLDWV
                     NAKTYPRIDKIELMANYFGVEKSDLVEVYSHKSVSSPSNIVVKLDKELKEPRHSDWIK
                     HGENLLSEQNTVEDSKNTVIDLFSYNYYDHAASAGTGQYLNDVQVETIELPVDYDADF
                     VIPVYGDSMEPKYHSGDYVFVKLSIDLSDGDIGVFEYYGDAYIKQLLINAEGAFLHSL
                     NSKYDDIPIDKDSDFRIIGEVVGSYSGNHNFTSYDYNRT"
     gene            1411..1569
                     /locus_tag="VCF75_001574"
     CDS             1411..1569
                     /locus_tag="VCF75_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547117.1"
                     /translation="MNWKKLMLGNLEHTFTSRDGKEKTSVEFEGGVLPALLVLGGITW
                     LIAWFITK"
     gene            1603..1812
                     /locus_tag="VCF75_001575"
     CDS             1603..1812
                     /locus_tag="VCF75_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547118.1"
                     /translation="MEGIEKVHDRRGRPDSLKVEQVIHLSILRGEGTGYDKVRMVEQY
                     YDMDGNLIFELDPCSEGYQEFLGLR"
     gene            complement(1788..2246)
                     /locus_tag="VCF75_001576"
     CDS             complement(1788..2246)
                     /locus_tag="VCF75_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEP2547119.1"
                     /translation="MNFNPISLAKSYPNYIPLTIREDYEEAHAILNLSPKASATLSRR
                     CLQGMIRDFWQVSKRSLSDEIEAINDLVDPSTRKVLDALRKIGNIGAHPEKDINLIVD
                     IEPLEAHKLLKFIELLMQKWYIERHDNETLLQDILDMDTNKQSQRKPKNS"
     gene            2533..2748
                     /locus_tag="VCF75_001577"
     CDS             2533..2748
                     /locus_tag="VCF75_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2547120.1"
                     /translation="MTKMTLKMLRVSKNWNQETAAKKLRISVSKLSNWENAKTFPDAI
                     EINKIEKLYDVNYSDIIFLPTKHGLTV"
     gene            complement(2840..>3249)
                     /locus_tag="VCF75_001578"
     CDS             complement(2840..>3249)
                     /locus_tag="VCF75_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547121.1"
                     /translation="DCYISRVDKGYDWMYDFSISNLSNVSVTIKKIELYNKDGKLISD
                     NGFNPFQKYEADMQNEADDYYGLSMPNYYMPLDYQWESSPFKSDTEVYPSSRENFSYY
                     LDEKPVKIKITTDKRIHKFRKYQLFFPHFDNNS"
BASE COUNT         1039 a          553 c          605 g         1052 t
ORIGIN      
        1 aaaaaacttt ccgtaaccat tgataatatt atagtaattg ttagtgagta gatacaattc
       61 tgctttttct tgattttcaa ttgttaagcc ccgatccctc aatatttgga tctgttcggt
      121 aattgatttg aattctttca aaaagaaaac ctcctccatt ataaaatgaa gaaggtttcc
      181 tccgtactgg tccccgtaga gataccagcg cttattttta gtatcttcat tttatattat
      241 attattattc ttgtcaacaa attatattca ttcttacgtt ctattataat cgtagctggt
      301 gaagttatga tttcccgaat aacttccgac gacttcgccg ataatcctaa agtcactatc
      361 tttatctatc ggtatatcgt catacttgct gttcaagcta tgcagaaatg ccccctcagc
      421 gtttataagc aactgtttga tataagcgtc accgtaatat tcaaacacgc ctatatcacc
      481 gtctgagagg tctatggaca gcttgacaaa tacatagtcc ccagaatggt acttaggctc
      541 catagaatca ccgtagactg ggataacaaa atcagcgtca tagtcgactg gtaattcaat
      601 cgtttctact tgtacatcat ttagatactg acctgtccca gcagaagcag cgtggtcgta
      661 gtagttgtaa gagaataggt cgattactgt gttcttacta tcttctactg tgttttgttc
      721 agacaaaaga ttttccccgt gttttatcca atcgctgtga cgaggttctt tgagctcctt
      781 gtctaattta acgacgatat tggatggaga agaaacgctc ttgtgagaat acacctctac
      841 taaatcagat ttttccaccc cgaaataatt agccattaat tcaattttgt caatccttgg
      901 ataagtctta gcgttcaccc agtctaatac tgtactatat ttaaaaccca ttacttttga
      961 aaattcttta ggattaatat ttttcttatc taaatgtctt cttatatttg aagccattat
     1021 ttttttatta ccgagcgaac cactcataac gacacctcat ttctgattat attataacgg
     1081 aaaaaccgtg tttttgtcaa aaaatatttt aaaaaataaa aaaaataacg tttttgtgtt
     1141 gacaacacgg tttaaccgtg ttaaactata atcaagctta agaaattaag caaaacaaaa
     1201 ggaggtgcag ccgatgaaat ctaggctaaa caaaaagcct aaacacaaag agctagaagt
     1261 cgaaatcaag attctttggt ttaagcttag aataaaatat ctgattacta ggtaatcgga
     1321 taaggggggt gaaactcccc cgccccgttt aggggtaagt ttagtttagc acattggctg
     1381 tatctccgca agaatgaaag gagaggttgc atgaattgga aaaagctaat gcttggcaac
     1441 ttagaacaca cgtttactag tcgtgatggc aaggaaaaaa caagcgttga atttgaaggc
     1501 ggtgtattac cagctctgtt agtgctaggt ggtatcactt ggctgattgc ttggtttatt
     1561 acaaaataaa aactcccaag agggagtgga aaggaggagg aaatggaagg aatagaaaaa
     1621 gtccacgata gacgtggacg gccggattct ttgaaagttg aacaagttat tcacttgtcg
     1681 attttaagag gggaagggac aggatacgat aaggttcgaa tggtggaaca atactatgat
     1741 atggatggta atctcatttt tgaattagat ccgtgctcag aagggtatca agaatttttg
     1801 ggcttacgtt gagattgttt gttagtatcc atatctaaaa tatcttgaag tagtgtctca
     1861 ttatcgtggc gttcgatgta ccatttttgc attaggagtt ctataaattt tagcaattta
     1921 tgagcttcta acggttcaat atcaacaatt aagttgatgt ctttttcagg atgtgctcca
     1981 atatttccga tttttcgcag tgcatctagg actttccgtg tactcgggtc tactaagtca
     2041 tttatagctt cgatttcatc agatagacta cgttttgata cttgccaaaa atctctaatc
     2101 attccttgta gacaacgtct tgaaagggtt gcagaggctt ttggacttag gttaagaata
     2161 gcatgggcct cttcatagtc ctctctgatt gtcagaggga tgtagttggg ataagatttt
     2221 gctagcgaaa tagggttgaa gttcattata cgatttcgga actgtctgcc cttgccaata
     2281 atatcgatag agacatttcg acaattagga caatccacaa gctggatagt tatttcgtca
     2341 cgcttgtgga aattttcatc atccgaaggg acaaattgag ggcggccttt tgaaaaattc
     2401 agattttctt ctcggaaggt atcgtaatgt tttggtacag gacaaccgca gaaaagacaa
     2461 aataaatgtt cagaatccat gattttctcc aatcatttta tttaaattat accatagaaa
     2521 gggggtggga gaatgacgaa aatgacgtta aaaatgctta gagtttcgaa gaactggaac
     2581 caagaaacag cagctaaaaa actaaggatt tcagtttcga aattaagcaa ttgggaaaat
     2641 gcaaaaacat ttccggatgc tattgaaata aataaaattg aaaaactata cgatgtcaat
     2701 tactctgata ttattttttt accaacaaaa cacggtttaa ccgtgtaacg aataaactaa
     2761 cacaaaacta gaaaggataa aagaaaaaat gctactaaca gcagcactta ttttgatttt
     2821 actaacgaac atcgctatct taactattat tgtcaaaatg gggaaagaat aactgatatt
     2881 ttcggaattt atgaatacgc ttatcagttg tgattttgat tttaactggt ttttcatcta
     2941 aatagtaaga gaaattttct cgactagatg gatatacttc agtatctgac ttaaatggcg
     3001 atgactccca ttggtaatct aatggcatat aataattcgg cattgataat ccataataat
     3061 catcagcttc attctgcatg tcagcttcgt atttttgaaa gggattgaag ccattatcgc
     3121 ttattagttt tccatctttg ttgtaaagtt caattttttt aatagtgact gaaacattcg
     3181 ataagttact gatactaaaa tcatacatcc aatcgtaacc tttatctact cttgaaatgt
     3241 aacaatcag
//
LOCUS       DATURV010000101         3232 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DATURV010000101 DATURV010000000
VERSION     DATURV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.101"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(390..1025)
                     /locus_tag="VCF75_001579"
     CDS             complement(390..1025)
                     /locus_tag="VCF75_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEP2547122.1"
                     /translation="MLTTNQIHKLLGVKEVYKAPDTLIKIILDKEKREDLFRQFLKYE
                     TDVSYDWFMQYFEEEQADRKNKKQDFTPKSVSTLLSKIISGNQYYEVAVGTGGILIQA
                     WQEQRLNDSPFTYRPSKYWYHVEELSDKAVPFLLFNMSIRGINGVVVHGDSLTRQVKN
                     IYFLQNTKDDMLSFSDINVMPRTQDIEREFNVKEWIGDGIEHIENPLIEWI"
     gene            complement(1025..1426)
                     /locus_tag="VCF75_001580"
     CDS             complement(1025..1426)
                     /locus_tag="VCF75_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547123.1"
                     /translation="MKNENFLGYDLAELLEEKLVGKRLKSIDDEKIVLADGTVIKIEL
                     NEGCGGCGNGWSELNITTENPNLESAVMGVEYTEKYSEWDDEFKIFVYMTDNSVIEIY
                     GYDGVGNGYYGYGFWVTVKNPGDLIESELED"
     gene            complement(1617..1952)
                     /locus_tag="VCF75_001581"
     CDS             complement(1617..1952)
                     /locus_tag="VCF75_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547124.1"
                     /translation="MDYLMIGIIVLSVVIFIMGFFILYYQAIIVNSMKNDFRRMRKEL
                     REELGFDSYNWSDSFRNMRRDIKSNKELISEINSLQIIKKAKEAKRLEELEQTKANVE
                     KEINKLNGG"
     gene            complement(1955..2227)
                     /locus_tag="VCF75_001582"
     CDS             complement(1955..2227)
                     /locus_tag="VCF75_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547125.1"
                     /translation="MTEEQELIKPLLEEIERKDDRLYMQSNVIAVLMTAVLMLLMISI
                     ALQNHYEPQITGLRTQLSRTQKQLKRASEDRTRQTKRIAELTGNGG"
     gene            complement(2230..2451)
                     /locus_tag="VCF75_001583"
     CDS             complement(2230..2451)
                     /locus_tag="VCF75_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547126.1"
                     /translation="MKIDYINFFEQVVPDWMRESNVKMQEVGFNTEAYWQWANQSIVA
                     ICEKYGNDSLINGQFHLIWEWLEDKANGG"
     gene            complement(2448..2843)
                     /locus_tag="VCF75_001584"
     CDS             complement(2448..2843)
                     /locus_tag="VCF75_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEP2547127.1"
                     /translation="MIEFFLPMDKIPTTTHQQKKVTVINGKPHFYEPESLKNARDKFT
                     SLLAQHVPPSKLDGPIRLTVKWLFPKIKGSTNGQYKTTKPDTDNLQKLLKDCMTELGF
                     WNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            complement(2836..3054)
                     /locus_tag="VCF75_001585"
     CDS             complement(2836..3054)
                     /locus_tag="VCF75_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547128.1"
                     /translation="MEIYKQRMIEEYKQLKKRAEKLSIVLNRYYLDELDFELSCPIEL
                     LQTQWHIMGAYLKILEQRFLVEGIYFND"
BASE COUNT          879 a          692 c          489 g         1172 t
ORIGIN      
        1 tgaggagact taacaagcga ccgactcatt aggttagcgt ctctttcaag ggtctctatg
       61 agatgtggga tagtctttaa ttcttccaaa aaattattag cttttgttgt cggaatgttg
      121 cccatctaac tctccattca tgatataata ttgttgggta tttaatcatg gaggcgttcg
      181 catggacgtc tttttttgtg gagaaaagtc ctctctttcc tttgtttttt gacacaggcg
      241 tacgatgtca gtattagcgc cttaaataat agccagtgac cgataaccag cgttagattt
      301 tgttttggtg taaggaggtc ctcgtttcta tttttcaatt tcggtcaata ccaaccgcac
      361 gagtcgaacg tgcgggatac cgttattggt tatatccatt caattagtgg attttcaatg
      421 tgttctatcc catcaccaat ccactctttg acattaaatt ctcgctcaat atcttgagtc
      481 cttggcataa cgttaatatc actaaaactc agcatgtcgt cttttgtatt ttgcaaaaaa
      541 taaatgtttt taacttgtct tgttaaagag tcgccatgca ccaccacacc atttatccct
      601 cttatagaca tattaaagag tagaaacggt actgctttgt ccgataactc ttctacgtga
      661 taccagtatt tactcggacg ataagtaaat ggactgtcat ttaatcgttg ttcttgccat
      721 gcttggataa gtatcccacc cgtcccgact gctacctcgt agtattgatt accacttatt
      781 attttagata atagtgtact aaccgattta ggcgtaaagt cttgcttttt atttttgcgg
      841 tcagcttgtt cttcctcaaa atattgcata aaccagtcgt aagatacatc tgtttcatat
      901 ttcaaaaact gtctgaataa gtcctcgcgt ttttccttgt ctaaaattat ttttatcaac
      961 gtgtcaggcg ctttgtagac ttcttttact cccagcagtt tgtgtatttg atttgtagtt
     1021 agcattagtc ctccaattca gattctatta gatcaccagg attcttcaca gtaacccaga
     1081 aaccatatcc ataatatcca ttaccaacgc cgtcatatcc gtaaatctca ataacagaat
     1141 tgtctgtcat gtagacaaaa attttgaatt catcatccca ttcactatat ttttctgtgt
     1201 attcaacacc cattacagca gattccaagt ttgggttttc tgtcgtgatg tttaattctg
     1261 accacccatt tccacaacct ccacaaccct cgtttaattc aattttaatt actgtcccat
     1321 cggcaagtac aatcttttcg tcgtctattg atttaagtcg tttccctact aatttttctt
     1381 ctaatagttc agctagatca taacctaaaa aattttcgtt tttcattttg tacctacctc
     1441 acttattttt tgttttatct atcgtcttgc taaaagtaaa cgaaacgatc ccggcggcta
     1501 tataatacag tatgcaagat attacaatta gaatagtcgc aaaaacactt aatataacaa
     1561 cagcaactaa aattttagct aaacagctta ttaaagctaa acatccagat accacttcat
     1621 cccccattca acttattgat ttctttttcg acgtttgctt ttgtctgctc gagttcttct
     1681 agtcgtttag cttctttggc tttttttatg atttgcaaag agtttatttc ggatattaac
     1741 tctttatttg atttaatatc tctacgcata tttctgaaac tgtcagacca gttatagcta
     1801 tcaaaaccga gttcttctct caattcttta cgcattctac gaaaatcatt tttcatcgaa
     1861 ttaacaatta ttgcttgata gtaaagtatg aaaaatccca ttataaaaat tactactgat
     1921 aatactatta ttccaatcat taaataatcc atccttatcc cccatttcct gtcaattccg
     1981 caatccgctt agtctgtctg gtcctatctt cgctagcacg tttaagctgc ttttgtgtcc
     2041 tacttagctg tgtacgtagt cctgtgattt gcggctcgta gtggttttgt aaggctatac
     2101 ttatcatcag taacataaga actgctgtca ttaagacggc aattacatta gactgcatat
     2161 acaatctatc atctttccgc tctatctctt ctagcagcgg ttttattagt tcttgctctt
     2221 cagtcatcgt taacctccgt ttgctttatc ctccaaccat tcccaaatca gatgaaactg
     2281 cccattgatc agactgtcat ttccgtactt ttcgcagatt gcaacaatag attgatttgc
     2341 ccattgccaa tacgcttcag tattaaatcc aacttcttgc attttgacat tactctctct
     2401 catccagtca ggaaccactt gttcaaaaaa attaatataa tcaattttca taactcttcc
     2461 acctttacgt atattcctac tgtatcagcc cagaacttct caataatttc cgaagcaacc
     2521 tgcgcatcat cattccaaaa tcccaattcg gtcatgcagt ctttaagcag cttctgcagg
     2581 ttgtcggtat ctggttttgt cgttttgtac tgtccgtttg tgctgccctt gattttggga
     2641 aatagccact tgactgtcag acggatagga ccatctaact tactgggtgg tacatgctga
     2701 gccaaaagac ttgtaaactt atccctagca tttttcaaac tctcaggctc gtaaaagtga
     2761 ggcttgccat ttatgacagt cactttcttt tgctgatgag ttgtcgtcgg gattttatcc
     2821 ataggcaaaa aaaattcaat cattaaaata aatcccttca accaaaaatc tttgttctag
     2881 aattttcaaa tacgctccca taatatgcca ttgcgtttgt aaaagttcaa ttgggcaaga
     2941 taattcaaaa tctaactcat caaggtagta tctgtttagc acaatagata gtttttctgc
     3001 gcgttttttt aactgtttat attcttcaat cattctttgt ttgtaaattt ccatttttac
     3061 ctcatctcca atttgtgttt tttatatgcg cctttgtcaa atatgggaca aaggacagag
     3121 ggggcgtagc tcagtcgccc ctcttccttt tccctttgac ttttgacgaa ggaaaaacat
     3181 tttataataa ctctgaaaga gttataggct gtattttctt agaaattaac ta
//
LOCUS       DATURV010000102         3176 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DATURV010000102 DATURV010000000
VERSION     DATURV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.102"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..189
                     /locus_tag="VCF75_001586"
     CDS             <1..189
                     /locus_tag="VCF75_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP2547129.1"
                     /translation="GGTQIYVNYSSVEQLKINIPTLKEQEAIGNFFQTLDQQIAQSEE
                     KLTELKALKQTLLNRLFV"
     gene            202..1782
                     /locus_tag="VCF75_001587"
     CDS             202..1782
                     /locus_tag="VCF75_001587"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP2547130.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLLAVCDNLEKHFNTFTEAQKIFEDAYQDEGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDSHIKKILDAYKSRDNFDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(2334..2939)
                     /locus_tag="VCF75_001588"
     CDS             complement(2334..2939)
                     /locus_tag="VCF75_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2547131.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDIILLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(2920..>3176)
                     /locus_tag="VCF75_001589"
     CDS             complement(2920..>3176)
                     /locus_tag="VCF75_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547132.1"
                     /translation="AIKYGDGKDIRLSLTIQSDIIIIEESNQVVEKVHSISYGRGLKS
                     FQETLAAFDGDLELQMDTKQFTIRILLPIDWKLCYEDFIN"
BASE COUNT         1078 a          594 c          485 g         1019 t
ORIGIN      
        1 ggtggcactc aaatctatgt aaattattca tctgttgaac aattaaagat aaatatacct
       61 acattaaaag aacaagaagc cattggtaat ttcttccaaa ctttagacca acaaattgct
      121 caatctgaag aaaaactaac agaactcaaa gcactcaaac aaacattact caatcgttta
      181 ttcgtataag aaaggacgca catggcagag aaaacaactt cactccggca agcactatgg
      241 cattcggccg accaattacg tggtcaaatg gatgccaatg actataaaaa ctatcttctt
      301 ggactcatct tttacaagca cttatctgat aaattactct tggctgtttg tgataatcta
      361 gaaaaacact tcaatacctt tacagaggct caaaaaatct ttgaagatgc ttatcaagat
      421 gaaggcctaa aagatgacct catttctgtt gttactggtg atttaggtta ctttatcgaa
      481 ccaacactga cctttgaaaa actcatccaa gatgtttatc acaatacttt ccaattagaa
      541 tcattagctc aaggttttcg cgatattgaa caaagtggtg aagactttga aaacttattt
      601 gaagatattg acctctactc taaaaaatta ggctctactc ctcaaaaaca aaatcaaacc
      661 atttctaatg tcatgaaaac attaaatgaa attgactttg aggcagtaga tggggatact
      721 ttaggcgatg cttatgagta cttgattggt gaatttgcca gtgagtcagg aaaaaaagca
      781 ggtgagttct ataccccaca agctgtctct cacttgatga ctcagattgt ctttttaggt
      841 cgtgaagatc aaaaaggaat gaccctctat gaccctgcta tgggatctgg atcactcctt
      901 ttaaatgcta agaaatatag taaccaatcc gataccgttt cttactatgg tcaagaaatc
      961 aatacttcta cttataactt ggctcgtatg aacatgatgc ttcatggcgt tgccatcgaa
     1021 aaccaacact taagtaacgc tgataccttg gatgcagact ggccaacaga tgagccaacc
     1081 aactttgatg gggtgttgat gaacccacct tattctttga aatggtcagc gactgctggt
     1141 ttcttaacag atccacggtt ttcaagttat ggtgtattag caccaaaatc aaaagctgac
     1201 tttgcctttt tacttcatgg tttctatcat ttaaaaaata caggaaccat ggcgattgtc
     1261 ttaccacatg gagttctttt ccgtggtgct gcagaaggca aaatccgcca aaaattacta
     1321 gaacaagggg ctatcgatac cattatcgga ctcccatcaa atatctttta taatacaagc
     1381 atccctacga ctattatcat attaaagaag aacagaacca acaaagatgt cttctttatc
     1441 gatgcctcaa aagagtttga taagggcaaa aatcaaaaca caatgacaga tagccatatc
     1501 aaaaagatcc ttgatgctta taagagtcgt gataattttg ataagttttc atatttagcc
     1561 tcatttgatg aaatcattga aaatgactat aacttaaata tcccacgcta tgttgatacc
     1621 tttgaggaag tcccagttaa accactgcca gaattagcta aacagctcag tgacattgat
     1681 caagaaattg ccaaaacaaa tgccaaacta gatcaactca tgaaacaact agtcggaaca
     1741 accaaagaag cacaagacga attggatact tttagaaact aaaagacaaa aaaacaccac
     1801 agtagatgat tactactgtg gtgttttatt taaaattgag tagtgaacct ttattgagta
     1861 cttatcatac aagattctcc ttcttcacct ataaatcaaa atagaatcat ctcatctaac
     1921 tttgtccgtt tctttgtttt atttaaaata gttatttgaa gttacaatga tttctcaaac
     1981 gtttagaaag cttaaacacg gttaagagaa attttcagga caacttttga tccttccaca
     2041 aaaaccatgg tgcgaaccat aaagtagttc caatcgttga tatataagca tatattaacg
     2101 ttctgggagt tgtgaagctt tacgagtttt tttagacctg gtttaaacaa cttttgtttg
     2161 actaaattta aaaagctttt catatcaggc atttccctac tttttataaa agcgttatct
     2221 caatcaattt caatcaaaaa gaactcaaaa acaatgagtt taaatctgag tatttaacag
     2281 gaaatcccta gcaacgacac aagtaaaact tatgtcgttt gattatctgc aactcagaaa
     2341 tccataaaat accctaattg ttcggctttg tagatagcct cttgcttatt atttaccaaa
     2401 agctttgagt agatgtgggt caaatgatta aatactgttc tcctactaat ttgtaacgtt
     2461 tcctctattt cttttataga gaatcctttc ttactcaaat ttaagatagc tatttcctgg
     2521 tcagttaaag attcaacata atcctgagat tcgatttgtt caaaatactt ttttccagaa
     2581 tccacttttt ttaagataga aatgagttgt ttagggtcta tatttttatc tacaaaaccg
     2641 tatgctccta ctttttttgc cctctctcta tagattgatt taacatatcc tgtcaacata
     2701 acaactttca aatgaggagt agactgtatg agttcttttg ctatctccaa tccattttct
     2761 ttagaaatat ttgttaaatt aatatcaagc aatataatat cgtacttaga caaatcaatc
     2821 gtcacatcgt taaaatgaga agtaatcgta tctatgacat ctacttcatc atactgttgg
     2881 aataacagtt ggatactctt tgcaaatagt ctgtggtcat caattaataa aatcttcata
     2941 acacaatttc caatctatgg gaagtaatat tctgatagta aattgtttag tatccatttg
     3001 tagttctaga tctccatcaa aagctgctag tgtttcttga aatgatttca atcctctgcc
     3061 ataacttata gagtgtactt tttccactac ttgattgctc tcctcaatga taataatatc
     3121 agattgaatc gttaaagata aacgaatgtc ttttccatct ccatatttta tagcat
//
LOCUS       DATURV010000103         3127 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DATURV010000103 DATURV010000000
VERSION     DATURV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.103"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            121..774
                     /locus_tag="VCF75_001590"
     CDS             121..774
                     /locus_tag="VCF75_001590"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP2547133.1"
                     /translation="MSSSKKVPGLLEAKPEIKFQFLKKNSVSLNSHHACQTLQVSRSG
                     FYAYLKRRPSSRHVENEALKEMIKAIFYEHKERYGSVRITQELCRRGIHVNYKCVGRL
                     LHQLGLYAKGSRYQYKYYNRRRSSLTRPNLVNQCFQATGKNKLWLGDLTYIPTQEGIL
                     YVSVLIDVYSRKIVGWAMGRRMQDKLVTEAFKQAYNREKPKEGVIVHTDQGSQYTGA"
     gene            814..1029
                     /locus_tag="VCF75_001591"
     CDS             814..1029
                     /locus_tag="VCF75_001591"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP2547134.1"
                     /translation="MNRKGNPYDNALMESFYKTLKRELVNDAHFATIKQAQLEIFKYS
                     ETYYNPKRLHSALGYLSPVEFEKIVTH"
     gene            1158..1310
                     /locus_tag="VCF75_001592"
                     /pseudo
     CDS             1158..1310
                     /locus_tag="VCF75_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1357..1887)
                     /locus_tag="VCF75_001593"
     CDS             complement(1357..1887)
                     /locus_tag="VCF75_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEP2547135.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(1880..>3127)
                     /locus_tag="VCF75_001594"
     CDS             complement(1880..>3127)
                     /locus_tag="VCF75_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HEP2547136.1"
                     /translation="SFSGGKDSGLLLNILLDFRDKYYPDREIGVFHQDFEAQYSLTTK
                     YVQETFTSLEGRKKVSLYWVCLPMATRTALSSYEMFWYPWDDKTEDIWVRPMPSQDYV
                     INLENNSITTYRYKMNQEDLAKQFGRWYKQIHGNQKTVCILGNRASESLHRYSGFINK
                     KYGYQKECWITKQFKDVWTASPLYDWSVEDIWHAYYKFSYSYNELYDLFYKAGLKPSQ
                     MRVASPFQDYAVDSLNLYRIIDQETWVKLLGRVQGVNFSNIYGRTKAMGYKSIALPKG
                     HSWKSYTQFLLSTLPVRLRNNYVRKFNKSIDFWHKTGGGLAEETINELIEKGYRIARN
                     GISNYTTFKHSRVIFLDQIPDDTDDIVTTKDVPSWKRMCFCILKNDHICRTMGFGLTR
                     EQQKIVAFLKEKYKTSLKELESE"
BASE COUNT         1023 a          624 c          469 g         1011 t
ORIGIN      
        1 atggattcaa gaatatgaaa aatatggaga aagtgcgttc ccaggacatg ggagcgcact
       61 tcgtcatgct caatttgaga taaaaaaact tgaaaaagag cgcaaactgc tacaggagga
      121 attagctctt ctaaaaaagt tccaggtctt cttgaagcca aaccggaaat aaaatttcag
      181 tttctgaaga aaaatagtgt tagcttgaat agtcatcacg cctgtcaaac tcttcaagtc
      241 tctcgttcag gtttctatgc ttatttaaaa cgtcgtccct cttcaaggca tgttgagaat
      301 gaagctttaa aggaaatgat taaagccatt ttctatgaac ataaagagcg ttacggaagt
      361 gtacgaatta cccaagaact ttgtagacgt ggcatccatg tcaattataa atgtgttggt
      421 agactccttc atcagctagg tctctatgct aaaggaagtc gatatcaata taaatactat
      481 aaccgtcgac gttcttcatt aactcgtccc aatcttgtta atcaatgttt ccaagcgact
      541 ggtaagaata aattatggct aggagactta acctacattc ctacacaaga aggaatactt
      601 tatgtgtctg tcttgataga cgtttatagt agaaagattg ttggttgggc aatgggccga
      661 cgtatgcaag acaagttggt aacagaagct ttcaaacaag cttataatag agaaaagcct
      721 aaagaagggg taatcgtcca tacagatcag ggttctcaat ataccggagc atgatttcag
      781 gacctactta gacagaaaaa atgtaagtcc agtatgaatc gaaaaggtaa tccatacgat
      841 aatgcactca tggaatcctt ttataaaaca ttaaaaagag agcttgtcaa tgatgctcat
      901 ttcgcaacta ttaagcaagc tcagcttgag atttttaaat atagtgagac ttactataac
      961 cccaaacgtc tgcattcagc tttagggtat ctctcacctg ttgaatttga aaaaatagtt
     1021 actcattagc ttaactttat gtctattttt cttgacaagt ccagggtatg aagtcccctt
     1081 atacacatac tttcaaaaca gctctcaatc ctctgtccgc tacaaaaata cgtcaccgct
     1141 gccctccagc tgcttcattc aacgacaaac ttgaagccac aaacagactc atcaagggca
     1201 ttaagtacaa tgcctttaga tttcagaact ttgacaactt taaaaccaaa atcctcatcg
     1261 ctttgaacat ccaaaaagag agaaccaatc tgattctctc tcgttgttaa tgataagtca
     1321 cccactacag ttgacaatga gtctcttact tcctgtttac tcatttaata gctcttctgg
     1381 ttcccaagat tgattaaact catggtcctt aaaaagagaa gccaatcctg ttatttgttt
     1441 cagtcttaaa atttcatctt tatccattcc taaatgtttt gcaatccagt tatcacttcg
     1501 cccacactca tgaagatcct ttacaatctg actcatcaaa tcaacatcat gacttcctct
     1561 agctctatta tgtcgaatgg tagaagccat tcgatagtct aatgacttat caataacaga
     1621 aacaggtaat ctcccacttt ccctttcata aatatctgaa taatctagca ttatacgata
     1681 tctatgaaaa ccatcaacaa tactataccg atcctcttcc ttatcgtaat agcaaacaat
     1741 cggcatagta taaccatcag attttatact atcatacaat aatttcattt caggcggagc
     1801 aactgaattc ggattataat catttggaga aatcttttca attggaattg atttaatctc
     1861 atagactgga ctaataaaat tactcacttt ctaactcctt taaactcgtc ttatatttct
     1921 cttttaaaaa ggctactatt ttttgttgct ctcttgtcaa accaaacccc atagttcgac
     1981 aaatatgatc attttttaaa atacagaaac acattcgttt ccagcttggt acatcctttg
     2041 ttgttacaat atcgtctgta tcatcaggaa tttgatctaa aaaaataacc cttgaatgtt
     2101 taaaagtggt ataatttgaa ataccatttc tagcaatacg atatcctttt tcaattagtt
     2161 cattaattgt ttcctccgca agtcctccgc ctgttttatg ccaaaagtct attgatttat
     2221 taaattttct aacataatta tttcttaacc tcactggtag cgttgataaa agaaactgag
     2281 tatacgactt ccacgaatga ccctttggta aagcaattga tttatatccc attgctttag
     2341 tacgtccata aatattcgaa aagttaactc cttgaacacg tcccaaaagc tttacccaag
     2401 tttcttgatc tattattcta taaagattta ggctatcaac agcatagtct tgaaaaggag
     2461 atgcaactct catttgtgat ggttttaaac ccgctttata gaatagatca taaagttcgt
     2521 tataagaata agaaaactta tagtaagcat gccatatgtc ttccactgac caatcatata
     2581 agggagatgc tgtccagaca tctttgaatt gtttggtaat ccagcattcc ttttgataac
     2641 catacttttt attgataaaa ccactatatc tgtgtaagga ttctgaagca cggtttccta
     2701 atatacaaac tgttttttga tttccgtgaa tttgtttata ccacctacca aattgtttcg
     2761 ctaaatcctc ctgattcatt ttataacgat aagtagtaat tgaattattt tccaaattaa
     2821 taacataatc ttgagatggc attggtctta cccagatatc ttctgttttg tcatcccacg
     2881 gataccaaaa catttcataa ctactaagtg cagttctagt agccatcggt aagcacaccc
     2941 aatacaaaga aacttttttt ctaccttcta aagaagtgaa tgtttcttga acatattttg
     3001 ttgttaaact gtactgtgct tcaaaatctt gatgaaacac acctatttct ctatccggat
     3061 aatatttatc tctaaaatcc agtaaaatat taagaagtaa cccactatct tttccacctg
     3121 agaagga
//
LOCUS       DATURV010000104         2965 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DATURV010000104 DATURV010000000
VERSION     DATURV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.104"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..774
                     /locus_tag="VCF75_001595"
     CDS             <1..774
                     /locus_tag="VCF75_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP2547137.1"
                     /translation="IQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYSQQLLKQFLT
                     VLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTGKYAKRIHYL
                     LELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTALIPVIGASI
                     GAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAMWVLMAITIG
                     ASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQKKQVS"
     gene            823..1503
                     /gene="radC"
                     /locus_tag="VCF75_001596"
     CDS             823..1503
                     /gene="radC"
                     /locus_tag="VCF75_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEP2547138.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTTFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(1505..2200)
                     /locus_tag="VCF75_001597"
     CDS             complement(1505..2200)
                     /locus_tag="VCF75_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEP2547139.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(2210..2854)
                     /locus_tag="VCF75_001598"
     CDS             complement(2210..2854)
                     /locus_tag="VCF75_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEP2547140.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
BASE COUNT          954 a          484 c          557 g          970 t
ORIGIN      
        1 atacaagaag ttgttaacaa tcttgagcac aataaactat tagcacgtac tattcagtat
       61 attggtggcg atggcaaact tacagagacg attgctaact atagtcaaca gttattaaag
      121 caatttctta ctgttttgac aaatatttta acatctgtca cagttattgc ttctgcaatt
      181 attaatcttt ttattagttt tgttttttcg ttatacgttt tagcaagtaa agaagatctt
      241 tgtcgccagg gaaatacttt agtagatact tatactggta agtatgctaa acgcattcat
      301 tacttgttag agttgttgca tcagcgtttt cacggatttt ttgttagtca gaccttagaa
      361 gctatgattt taggttcgct gactgctagt ggcatgttta tattaagatt accatttgca
      421 gggactattg gcgttttagt agcttttaca gcattgattc cagttatagg tgcttcaatt
      481 ggagcagcta ttggatttat tttaattatg actcagtcca tgtcacaggc cattattttt
      541 atcatttttt taattatttt gcagcagatt gaaggcaatt ttatttatcc gaaagtagtt
      601 ggtggatcga ttggattacc ggctatgtgg gtattaatgg caattacaat aggcgcttct
      661 ttaaagggaa tagttggtat gattattgca gttcctttag cagcgacact ttatcaagtg
      721 attaaagata atattcaaaa aagacaagct attcaaaaaa aacaagtttc ctaaaaggaa
      781 cttgtttttt tgtcgaataa aaagatggag gtaatatgat aaatgtattc tataaaatgt
      841 gatgataata aagccatgcc aagagaacgt ttgatgcgac taggagcaga gtctctaagt
      901 aatcaagaat tattagcaat tttattacga acaggtaata aagaaaagca tgtcttagag
      961 ctgtcatcct atcttttatc gcatttagac agtctggcag attttaaaaa gatgtctttg
     1021 caagaattac aacatttggc aggtatagga aaagttaaag cgattgaaat taaagctatg
     1081 attgagttgg tttcccgaat tttagcgaca gataagacat taactgatag cgtattaacc
     1141 agtgttcagg tcgctgaaaa aatgatggca gctttaggag ataaaaaaca agagcattta
     1201 gtcgtattgt atttagataa tcaaaatcgt attattgaag aaaaaactac ttttattggg
     1261 actgtccgac gttcacttgc agaaccaaga gaaattttat actatgcctg taaaaatatg
     1321 gcgactagtc tcattgttat tcataatcat ccttcaggaa atattgaacc tagttctaac
     1381 gattattgct ttactgaaaa aataaaacga tcatgtgaag atttaggcat tatctgtcta
     1441 gatcacatta tcgttagcta taaagattat tatagttttc gagaaaaatc aacccttttt
     1501 taaatcagaa gtcattgacg aataagcgga acaattgtag atcttcatct cgaacacctt
     1561 ggagaagttc aggatgccac tgaacaccta aaaaaggaat ggcatcgttg gttgatatga
     1621 ctgcttcgat ggtaccatca cgtggatctc tagcgattac ttttaaatcc ttagcaacag
     1681 tttttaaact ttggcgatga aaagaattaa ttaatgtctt atgaccgtaa ataggatata
     1741 agatagagtc tggctcaata atcatttcgt gtgataagaa atcagaaggc gcttcttgcc
     1801 aatgtgagtc aatgtgttgg ttaagatttc ctcctaaggc aacattcatt aactgtgtac
     1861 cacgacaaat ccctaagatt ggttttttta atgttatagc ctccttgatg atagctagct
     1921 caaatgtgtc tcgctctggt gaaaaatcat catcaaaagc agccttctct tcttgataat
     1981 attttgggtc aacgttttgc ccaccaatta aaatgatttt atcaaccata gatacataag
     2041 ttttagcggc ggcttcatca ccaatgggta aaagaagcgg cagcccccca gattgagtga
     2101 ctgcctgtac aaatccagta ggtgcatatg accatgggag attatccaaa gccatattta
     2161 gtctttgatt tgcagttatt ccaataatcg gtttggtcat tatacctcct taatctagat
     2221 gattttgatt cataaaatag agtagtgtct ggagctcact ggtcaaatcg acagattgaa
     2281 caataactcc tttaggaact tgtaggtgaa caggagcaaa actcaagatt cctttgattc
     2341 ctgcttcaat aagttgatca gtaacttctt gagcatgaat gctgggaacc gttaagatag
     2401 ccgtttcaat gtcagtgttc gcaatccgtt ctttgacaga tgagatacca tggactggaa
     2461 tattatcagc tgttttagta ccaacaagag cgttatcatc ggtatcaaaa cccatggcaa
     2521 tttgcatctt attacgatca tggaagcggt aatgtaatag agcacgccct atattaccgc
     2581 atccaactaa gataacgttt gttgtagagt gatcattaag caagtcagca aaaaaattca
     2641 tcaatttggt aacatcatag ccaaacccac gacgccccaa ttctccaaaa taagaaaagt
     2701 cacggcgtac agtagcagag tcaattccca ttgcatctgc aatttgttta gaactggctt
     2761 tttcaacttg gtcagcatgg aatcgtttaa aaatacggta ataaagtgat aagcgctttg
     2821 cagtcgcttt tggaatagat ttgtcaataa ccactatttt actccttgta gatttctcgt
     2881 taaatatgtc tgtctttatt gtaacaaaaa gattgtgaaa aatgcaacat tttgattgtt
     2941 tttgtatgag ttaaaaaagc taaaa
//
LOCUS       DATURV010000105         2900 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DATURV010000105 DATURV010000000
VERSION     DATURV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.105"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            245..916
                     /gene="sdaAB"
                     /locus_tag="VCF75_001599"
     CDS             245..916
                     /gene="sdaAB"
                     /locus_tag="VCF75_001599"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEP2547141.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            928..1800
                     /gene="sdaAA"
                     /locus_tag="VCF75_001600"
     CDS             928..1800
                     /gene="sdaAA"
                     /locus_tag="VCF75_001600"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEP2547142.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKKQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIESQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(2214..2828)
                     /locus_tag="VCF75_001601"
     CDS             complement(2214..2828)
                     /locus_tag="VCF75_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEP2547143.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKEKAKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
BASE COUNT          904 a          524 c          527 g          945 t
ORIGIN      
        1 atattatgat tctttttctt atcagttaac gtacgattga aggtcgtcgt tttcaaaagt
       61 cgatttgaag gtcgattttg aagcgccatc agcgcaaacc ttattataac aattactgcg
      121 tggaatgcca atcattttat tgctcaataa ttttttatta taaaaataat tttttataat
      181 tatattgctg ttttttgata tttttttgtt ataataaaca tagaaataaa aaacggagtt
      241 gcctatgaat acacaaaaat ttcaatcagt ttttgacatc attgggccag taatgattgg
      301 tccatcaagt agtcacacag ctggtgccgt cagaattggt aaggtagtcc attccatctt
      361 tggagatatt cctgatgaag tgacgtttca cctttataat tcatttgcta aaacctacag
      421 aggacatggt actgataagg ctcttgttgc aggaattatg ggtatggata ctgataatcc
      481 tgatattaaa aattctctag aaattgctca tcaaaaaggg ataaaaatct attgggatat
      541 cttaaaagat agtaatgctc cccatcccaa tactgttaaa atcagtgtca aaaaagctga
      601 caaaacactt agtgttactg gtgtttcaat tggaggagga aacattcaag taacagaact
      661 aaatggtttt tctgtctcgt tatccatgaa tactcctact atagttactg tacacaaaga
      721 tattcccgga atgatcgcaa aggtaactga tatcctatct tccaataata ttaatatcgc
      781 cacaatgaat gttacacgtg aatctgctgg tgaaaaagct actatgatta tagaagtcga
      841 ttctagagag tgccaagaag cagccaacca aattgcaaaa attcctcata tttataatgt
      901 caacttcttt gactagaaag gaaagctatg ttttatacta ttgaagaact tgtcaaacaa
      961 gctgaccaac aatttaatgg aaatattgct gaactcatga ttgccacgga agtggagatg
     1021 tctggaagaa atcgagaaga tattatcaaa atcatgtcac gcaatttaca agttatgaaa
     1081 gcagctgtaa cagaaggact aacctcaacc aaatctatca gtggtttgac agggggcgat
     1141 gcggtcaaaa tggataacta cattaaaaaa gggaacagcc tttctgatac tactattctt
     1201 aatgctgtta gaaatgctat agctgtaaac gaattaaatg ctaaaatggg acttgtctgt
     1261 gcaacaccca ctgcaggaag tgcaggatgc ttaccagcag tgctcgcaac agctattgaa
     1321 aaacttgact tatcagaaaa aaaacaatta gagtttttat ttactgcagg agcttttggt
     1381 cttgttattg gaaataacgc ctctatttct ggtgcagaag gtggatgcca agcagaagtt
     1441 ggttctgcag ctgctatgag tgcagctgct cttgttaaag cagctggtgg aacatctcat
     1501 caagctagtc aagctattgc gtttgttatc aaaaatttat taggacttgt ctgtgatcct
     1561 gttgccggcc tagtagaagt gccttgtgtc aaacgcaatg ccctaggagc aagtttcgcc
     1621 cttgtcgcag ctgatatggc tttagctgat attgaatctc aaattcctgt tgacgaagtt
     1681 attgatgcta tgtatcaagt cggctctgcc atgccaactg ctttccgtga aacagcagaa
     1741 ggtggtttag ccgctactcc aactggacga cgttacagcg tagaaatatt tggcgaataa
     1801 atagatagtt agaaaaattc ttagaaatat ttgaaagcgt tttatttttc tgctatatat
     1861 tcactaaaat gactaataga tagctatcaa gctatgttgt tttcccttag aaacaactcc
     1921 tagtaaattg ttaaaactca aaagtgtagt agtgtaaaat actgaaaatt gctaatgacc
     1981 ttataaacat ttaaaagttt ttttcctgct tgttatcagt ataactgcct taaatatcag
     2041 aggcacttaa gtgctggtca gttttcctaa ataatacgag ctatattccc tgataaggca
     2101 atgttttgtt ttgagactaa ttcatcacta caaatcataa agacaattaa ataccaaaaa
     2161 cctattgtta gtaaatacta acaataggtt ttatttcttc cagctatgtt tatttagtaa
     2221 ccccaagctg ataaaccttg tgcactatag gctttcaagg ctgcattgac ttgatcttca
     2281 accgttgctg tagaacccca acctggcatt gtctggaaca accctgatgc cccagaagca
     2341 ttagctgcgt taggatttcc attagattca cgcgcaatta tatgttccca agttgattgt
     2401 ggaacacctg ttgctgctgc catctgtgcc gccgcttgac ttcctacaat accagcagta
     2461 tttccattgg aaagtaccac attgtttgga ttataggcta ctgaagatac ctgctgaaca
     2521 gtttgagtca cttcttgctg aacaaccggt gcttcctctg ttactgtcgg agctacagag
     2581 ttttctactg tgggaatagg ctccgaagca atgctctttg ctttttcttt gctttcttct
     2641 ttagtaatag catgattatt ttttggagcc ttgtcgattg ttaccatttt actttctgac
     2701 ttcttagcat aagacttagt atcagcattt ttacttgaga aggcaaaaat gatggctaat
     2761 attgttagag ctaacgctac taatccaaaa ttaaaataac gttgttttaa attttctttc
     2821 ttaaacattc aataatacct cacttttctc cgttatccat aatatctttt aattattacc
     2881 tcgttattga agatatatta
//
LOCUS       DATURV010000106         2762 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DATURV010000106 DATURV010000000
VERSION     DATURV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.106"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            101..751
                     /locus_tag="VCF75_001602"
     CDS             101..751
                     /locus_tag="VCF75_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP2547144.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETQFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            754..1422
                     /locus_tag="VCF75_001603"
     CDS             754..1422
                     /locus_tag="VCF75_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2547145.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            1431..2663
                     /locus_tag="VCF75_001604"
     CDS             1431..2663
                     /locus_tag="VCF75_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP2547146.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPDTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSMMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
BASE COUNT          917 a          410 c          546 g          889 t
ORIGIN      
        1 aaactataca gatagtttgc agttatatag ttagaaataa agataaccga tcattgcaga
       61 gatttaagca tttgttgaga aaatttgtta taataagcgt atgaaagaaa gactagaaca
      121 ttattctacc agctctatat atagcggatt tgaagtgtac ttgccgttgg tactacagac
      181 aataacagat gtgattattg ctgagaatat caaaagcaaa aaagaaacag ggtttaaact
      241 atacgaacac tttacatccc gtattaaatc ggaagcttcg atgattgaaa aatgtcaacg
      301 caaacagctt cctctaacaa gcaaatctgc gttaaaaatc attaaagata gtatcggtat
      361 tcgcattatt tgtggtttta ttgatgatat ttatcggatg gtagacttgc tcaaaagcat
      421 tcctggtatg tcagttaaca ctgaaaaaga ttatatttta aatgccaagc ctaatggcta
      481 tagatcatat catctcattt tggaattaga aacacaattt ccagatattt taggggaaaa
      541 aaaaggatgt tattttatag aagtgcagtt gcgaaccatt gctcaagact cttgggctag
      601 cttggaacat caaatgaagt acaaacatca agttgctaat gcagaaatga ttacaagaga
      661 gttaaagcgt tgtgcagacg agttggcatc ttgtgatgtt accatgcaaa ccattcgtca
      721 attgattcaa gaaacgacgg aggaagagta accatgaaaa ttttattagc tgaagacgaa
      781 tggcagatgt ctaatgtttt gacaactgcc atgactcatc aaggttatga cgttgatgtg
      841 gtttttaacg gtcaagaggc aattgataaa gccaaagaca atgcatatga tattatgatt
      901 ttagatatca tgatgccgat taagtctggc attgaggctt taaaggagat tcgcgcttca
      961 ggaaattgct cacatattat tatgctgacg gctatggcag aaattaatga ccgtgttacc
     1021 ggactagatg caggagctga tgattatctg actaaacctt tctcattaaa agaactttta
     1081 gctagattaa ggtcgatgga aagacgagta gaaagtttta ctcctcaggt attacaattt
     1141 gctggtgtca cattaaatat taatgagcaa gaactgagtg ccggtaatgc tatacgatta
     1201 gcttctaaag aaggtaaatt aatggccttt ttaatgttaa atcaaggaaa gtacttggat
     1261 actaaaacac tttaccaaca tgtttggtca gaccaagaag attatgatac ttctattgtt
     1321 tgggtttata tttcttatct tcgtcaaaaa ttattagcta ttcaagcaaa tgtcattatt
     1381 gcaggtgata aagatactag ctattgttta ttagaaaaat aggaggtgac atgtttaatc
     1441 gtatccgtat tcgttttatc atgattgctt ctattgccat ttttattatt ttaagttcaa
     1501 ttgttggtat tattaacacg gcacgctgtt accaaagtca acaagaaatt aaccgtattt
     1561 tacatttaat ttcctcaaat aaaggtaaat tacctgacac tacggaaagc tctaaacgac
     1621 ttggaacgaa actgtctgaa gatagcctaa gccagtttcg gtattatagt gtcattttta
     1681 atgctaatgg acatctactt tcttctaata ctgctaatat ttcagcttta gatagagaag
     1741 aagcccaata ttttgctaga ctttttgcta agtctgggga agaaaaaggc agttaccgtc
     1801 accaagatag tgtttattcg tacttgatta ctcagctgcc aaatgaagaa aaactggttg
     1861 ttattttaga tactaccttt tattttcgta gtgtaggaga tttattagct gtttcaatga
     1921 tgttggcctt tggaggattt atcttttttg ttgttttagt gagtcttttt tccggtatgg
     1981 tcatcaaacc ttttgtccaa aattatgaga agcagcgccg ctttattact aatgcaggcc
     2041 acgagttaaa aacaccacta gccattatct cagccaataa tgaattggtc gaattaatga
     2101 ctggagaatc agaatggacc aagagtacaa gtgatcaggt gaagcgattg acaggcttga
     2161 ttaatcaaat gattactttg gctcgtttgg aggaacagcc agatgtggta ttacatatgg
     2221 tagacttttc agctattgct caagatgcag ctgaagattt taagagtctg gttttgaaag
     2281 atggtaaacg ttttgatttg acgattcagc cgaatattat gattaaagcg gaggaaaagt
     2341 cattatttga acttgtgaca attttagttg ataatgccaa taaatattgt gatcctaaag
     2401 gcttggtcaa ggtatcttta accactattg gaagaagacg aaaacgagcg aaattagaag
     2461 tttctaatac ctatttggaa ggaaaatcca ttgattacag ccgctttttt gaacgctttt
     2521 atcgcgaaga cgaatcccat aatagcaaag aaaaaggtta tggaattggt ttatctatgg
     2581 cagaaagtat ggttaagtta tttaaaggaa cgataactgt aaattataaa aacgatgcta
     2641 ttgtttttac agtggtgatt tgacttaaac atttttcagt acctttagct agtaataatg
     2701 tctaggtaaa ggtaataaac gatgcttaac cagcccatga ggctggtttt taacgtttaa
     2761 tt
//
LOCUS       DATURV010000107         2662 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DATURV010000107 DATURV010000000
VERSION     DATURV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.107"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(260..2239)
                     /gene="ftsH"
                     /locus_tag="VCF75_001605"
     CDS             complement(260..2239)
                     /gene="ftsH"
                     /locus_tag="VCF75_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEP2547147.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     AYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMTESESAE"
     gene            complement(2261..>2662)
                     /gene="hpt"
                     /locus_tag="VCF75_001606"
     CDS             complement(2261..>2662)
                     /gene="hpt"
                     /locus_tag="VCF75_001606"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEP2547148.1"
                     /translation="FMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIE
                     GRDIIIVEDIIDTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIP
                     NEFIVGFGLDYAENYRNLPYVGVLKEEVYSK"
BASE COUNT          815 a          560 c          387 g          900 t
ORIGIN      
        1 catcttattg gttgactgaa acttttgcta tcttctgttc ttctattatt ctaagaaaac
       61 actaatagat tttttcaaat ggctttaaca attttctagt tttaaataca aaagtctttc
      121 tcactttaag taataaagaa aaaaagtatg tcatcttctc atttctagca attaggcacc
      181 tattacaatt cataacgttt acaaggatat tgtgttgata aaaaagctga acttaatcta
      241 taagttcagc ttttttatct cattctgcag attcagattc tgtcatttta tttttaattt
      301 cgtcataaga taacgcatga gcttcattat catcagtttc taaatcaact ggcattttac
      361 cagtttcata aattgattta atttgtgcag cgtctagagt ttcatatttg agaagagctt
      421 cagcaatcaa tttatgagta tctcgattct cattgatgat atcagctgct tgattacgag
      481 cttgatttaa gagttcacga acctcatcat caatcatttg agctgtttgg gcagagtaag
      541 ctttttcagg agagatctgt cctggcatca tcgcatggtt accttcatat tgaactggtc
      601 caagcttttc actcatacca tattccgtaa ccatggccct tgctatctga gtagcttgct
      661 caaagtcatt tgacgcacct gatgtttgag cattaaaaac aatttcttca gcaacacgac
      721 cacccatcaa tcctgctaat tgctctttca gatcttcttt agaaagaagc atttgatctt
      781 cttttggaag agcgatcata taaccacctg ctcgtccacg aggtacaata gttaccttgt
      841 gaactacacg cgcattagat aaaactaaac caacaatagt atgaccagct tcgtgataag
      901 caaccatttc tcgctctttt tgcgaaatag tgcgatcttt ctttgatggt ccagcaataa
      961 cacgatcttc agcttcatca atatcactag catcaatttt aattttattt ctgcgagcag
     1021 caactaacgc agcttcattt aaaacatttt caagatcagc acctacaaaa cctggagtct
     1081 gttgggctac tactttcaaa ttaacgtcat ttgcaagtgg tttattttta gcatgaacac
     1141 gtaaaatggc ttcacgtcct ttaacatcag ggcgaccaac taaaacctta cgatcaaaac
     1201 gacctggtct tagtaaagct ggatctaaaa catcactacg gttagttgca gcaataacga
     1261 taatgttctc attaccctca aaaccatcca tttcaatgag taattggtta agggtttgtt
     1321 ctcgttcatc gttaccgcct cccatgccag caccacgacg acggcctaca gcgtcaattt
     1381 catcgataaa gatgatcgca cgctcagctt ttttagcatc ctcaaataaa gaacgaacac
     1441 gactagcacc tacaccgaca aacatttcga caaaatctga acctgaaata ctgaaaaatg
     1501 gcactccagc ttcacctgct acagctttag ctagtaatgt tttaccagta cctggaggcc
     1561 cctctagaag aactcccgct ggaatccgtg ctcctagtga tttatatttt ttaggatttt
     1621 tcaaaaaatc aacaacttct actaattctt gtttttcttc ttcggcacca gcaacgtctg
     1681 taaatctcac tttaacatca ccttttgatt gagattttgc cttatttttg ccgaaactca
     1741 tagcaccacg agctcctcca ccaccttgat tcatcatcat catcatgaaa gcagcaaaaa
     1801 taacaatagg taaaaagctc attaagaagg taatccaagt accacttgaa ctttcctgct
     1861 taactgtaag ctctgttcca tttttatcag ctgctgctgt catttctttc aagatggtat
     1921 cactaggtaa tactaaagat gagaactctg ttacctgtgt tgaagctctt ccacctaaaa
     1981 atgatagtcc tgaattaact gtcacttttt gaggtttctc atatttccct ttaacttcaa
     2041 taatactacc gctaggctga taacttaaag attttatatc tccagcctta agatgtttaa
     2101 ttaatttgga ataactaatc tgttggcttt gtgtgctggt tcccttaaga taaaattgaa
     2161 atcctgtgat gacaacaata atcatcaata tatatataaa agaattttta acaaaaccat
     2221 tatttttatt atttttcata cagtaaacat tgaccttttt ctattttgaa tagacttctt
     2281 cttttaatac acctacgtaa ggaagattgc gataattttc tgcgtaatct aaaccaaagc
     2341 ctacgataaa ttcatttgga atattgtaac aaacatagtc tgcttcaatt ttaacaacac
     2401 gaccttcagg tttatcaaac agtgttgcaa ttttgatagt attagcttta cggtacttaa
     2461 acatatctcg taaatatttt aaggtgcgac cggtgtcaat aatatcttca acaattatga
     2521 tatctcttcc ttcaatattg gtatctacat cttttaaaat tttaacttca ccactgctag
     2581 atgtaccgcc atgataactt gatactacca taaaatcaat ttcaacatga gtatcgatgt
     2641 gtttcattag ttctgccata aa
//
LOCUS       DATURV010000108         2442 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DATURV010000108 DATURV010000000
VERSION     DATURV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.108"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            192..926
                     /locus_tag="VCF75_001607"
     CDS             192..926
                     /locus_tag="VCF75_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEP2547149.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIA
                     SKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIKGLDYKWSENTSIKEALNE"
     gene            919..1905
                     /locus_tag="VCF75_001608"
     CDS             919..1905
                     /locus_tag="VCF75_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEP2547150.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKIFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIFVNAIYIKDLEKHDYSIEILS
                     CINQTVIKIISNLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSELVT
                     GTNTFQIHDDMMDTYISLLEGNAIPEDCYINNAIEITKLLINLYSECEKIYL"
     gene            1915..>2442
                     /locus_tag="VCF75_001609"
     CDS             1915..>2442
                     /locus_tag="VCF75_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEP2547151.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEE"
BASE COUNT          912 a          299 c          473 g          758 t
ORIGIN      
        1 tgatttagga atacattatg tagaactagg taaaggaatt atcgattata aaacaatcat
       61 tcatgaaatc catgaacatt ttatagactt aatttttagt ttagaagtac atgaaaaaga
      121 aatagacaaa aaggcagaag tagtgaggaa gtcttatctg acactaaatg agtatattaa
      181 tggaggctaa aatgaatttg agtatatttg gtttaaagaa tattccttat ttgaaagaag
      241 gggatagtat agaaaagctt attgaagaaa gcattaaaac ttcagaattt tttatcgaag
      301 acaatgatgt gctttgcata gcttctaaag ttgtatctat cgcagaaggc caggtgatgt
      361 ctttaaacga gatacaagtg agtgatgtag caaaagaaat ccacaggaat attcctcgta
      421 aagatcctag aataattgaa attatgctaa atttagtcaa tagagatctg tcacgtttag
      481 atatcaaaaa aaattacatt ggttgtcgct tggaaaatgg tttaaagttg acaagtggtg
      541 gaatcgatag aaaatcagta gatgaagtat ttcttttacc taataatccg gatgcttctg
      601 caaaaagaat ttcggaatat cttaaaaaat cacttggaaa aaatgttgca gttgttataa
      661 cagatagtga tggtagagag gataaaagag gtgctactca agtagcaatt ggtatttatg
      721 gtattcatcc tttgagaaaa acagaagtaa tagattcaca aggagagaca ataaaattcc
      781 aagaagagac tttgtgtgat atgattgcag cttgtgctgg cttagtaatg gggcaacgtg
      841 gaactggaat tccagctgtc ctcattaagg gcttggatta taaatggtct gaaaatacta
      901 gtattaagga agcgttaaat gagtagaaat caggactgta agaatattct aatcattggt
      961 tttggaaaaa ttgggaaaat taaagcgtac aagtggctat cacgcggata tgaggtatat
     1021 gtcaaagatt tgaaattctc cagcatgaaa taccttaata caaatagtga tgtgttggat
     1081 gatttatcac gacaatattt tactattgag atttgtactc cgacaaatca ccaccttacg
     1141 ctattgaaaa ccgttataac aaggtatgtc tattcttata ttagcattga aaaaccactg
     1201 tgtaacaggt tagaagattt agaagaaatg aagtcgctag tagaaagtca cccacatcta
     1261 gcaaaaaaaa tttttgccag tgaacaatac ttcttttcga aaattttgga aaaactgtta
     1321 caagtagatt gtttctctct agataaaatc aaggaaataa cagttaattt ttctaaagat
     1381 aggattgagg ataacgagaa tgggagattt ttagataaag agatattggg ctatggaata
     1441 gagattcctc atatcattgc tgtcatttct tatttaggaa tttcattaga agaattcaaa
     1501 aaaggaatct ttgtgaatgc tatttatata aaagacttag agaaacatga ttattcaata
     1561 gaaatattat cttgtattaa tcaaacagtg attaaaatta ttagtaattt gggaagtttt
     1621 gcaataagag agggcaagat tgaaggaggt gatgctaaaa cagtaagaag agttaccatt
     1681 gatgacaaaa caatactatt tgatccacat cctaaactcg agaggtataa gagtgaattg
     1741 gtgactggga caaatacgtt tcaaatacac gatgatatga tggacacgta tatcagtttg
     1801 ttagaaggaa atgctattcc tgaagattgc tatatcaata atgcaattga gataacaaaa
     1861 ttattaatta acttatattc agaatgtgaa aaaatttatt tatgaggtgt aactatggat
     1921 ttaaaaatta caaacggctt ttatgaccca agtcatttat catacgaagt agtagaacgc
     1981 aaaggcttag gtcaccctga taccttagca gatggaattg ctgagcaaat tgaaattgat
     2041 tactcacttt attgtttgga caagtttggt gttattccac atcataattt tgataagatt
     2101 attatccgag ggggacattc agttcaggat tttggtggaa gtgactttat tgaaccaatt
     2161 aaaattattt ttctaggtag ggcgagcaaa aaatgtttta atagttcaat tccacttttt
     2221 aaaattcaga aaaaggctgc aactaaatat ttaaatagaa ttttacctaa tttagatgtt
     2281 gaaaattatg tagaatttga gacattgacc tcagatttta ctacaaaaac taattggttc
     2341 tcgcctgaag caatagaaga tttaccggag tatctagatg taccaaaggc aaatgataca
     2401 gctacaatga taagttattg gccattaaca atttctgaag aa
//
LOCUS       DATURV010000109         2282 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DATURV010000109 DATURV010000000
VERSION     DATURV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.109"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            13..774
                     /locus_tag="VCF75_001610"
     CDS             13..774
                     /locus_tag="VCF75_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2547152.1"
                     /translation="MRTNSEIISLIQSKVEERKMSMSELARNVGIAKSTMSRYFNKTR
                     EFPLNRADDFAKALNISTEFLLGIDLNSEVDGSELLGIYRELEEQRRVIVLDTAKEQL
                     EEQTKIINFSDKKTDIEDIFEVKGTTFAAAASGFGRGFEADDYDTYTVYTDEEPPHYD
                     YAIGIRGDSMLPKYEQGDMLYIVDKGMSTYSGQLCIVVHNGNTYFKKVYTEHDGLRLV
                     SLNKKYSDIFIGYPPAEDTYIKIYDVVGSFTPIEF"
     gene            801..1166
                     /locus_tag="VCF75_001611"
     CDS             801..1166
                     /locus_tag="VCF75_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547153.1"
                     /translation="MTLKQLLKSKNLGEYFEAKNNAEKMNEIENRGLKQVMKNSVRQT
                     QLNSEIKQVIKKEKEAVRCPKCKSTNVGFMQQGKKTFSVKKAVAGTLLIGGTVMGFLG
                     EKGKKQWHCNECSCIFETK"
     gene            1290..>2282
                     /locus_tag="VCF75_001612"
     CDS             1290..>2282
                     /locus_tag="VCF75_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP2547154.1"
                     /translation="MRKVAIYSRVSTINQAEEGYSIQGQIEALTKYCEAMEWKIYKNY
                     SDAGFSGGKLERPAITELIEDGKNNKFDTILVYKLDRLSRNVKDTLYLVKDVFTANNI
                     HFVSLKENIDTSSAMGNLFLTLLSAIAEFEREQIKERMQFGVMNRAKSGKTTAWKTPP
                     YGYRYNKDEKTLSVNELEAANVRQMFDMIISGCSIMSITNYARDNFVGNTWTHVKVKR
                     ILENETYKGLVKYREQTFSGDHQAIIDEKTYNKAQIALAHRTDTKTNTRPFQGKYMLS
                     HIAKCGYCGAPLKVCTGRAKNDGTRRQTYVCVNKTESLARRSVNNYNNQKICNTG"
BASE COUNT          900 a          325 c          455 g          602 t
ORIGIN      
        1 ggataacaaa acatgagaac aaacagcgaa attatttctt tgatacagag caaagtagaa
       61 gaaagaaaga tgtcaatgag tgagttggct agaaatgttg gtattgcaaa atcaacgatg
      121 tctagatatt ttaataaaac aagagaattt cctttaaaca gggcagatga ttttgctaaa
      181 gcacttaata tatcaacaga gtttttactt ggtattgatt taaatagcga agtagatggt
      241 tcggaactgc ttggaatata ccgcgaatta gaagaacaac gaagagtaat tgttttagat
      301 acagcaaaag agcaattgga agaacaaaca aaaatcatca atttcagtga taaaaaaact
      361 gatatcgaag atatttttga agttaaagga acgacttttg ctgcagcagc aagcggattt
      421 ggtagaggtt ttgaagcaga tgactatgat acttatacag tttatacaga cgaagaacct
      481 ccacattatg attatgctat tggcataaga ggcgatagca tgttaccaaa gtacgagcaa
      541 ggagatatgc tttatatcgt tgataaagga atgtcaacat acagcggaca attatgtatt
      601 gttgtacata acgggaacac ttattttaaa aaagtttaca ctgaacatga tggacttcga
      661 ttagtatcac ttaataaaaa atatagcgat atttttattg gctatccacc agcagaagat
      721 acatacatta aaatatatga cgttgttggt agttttacac cgattgaatt ttaactaatg
      781 tttttagaga ggacaatgat atgacgttaa aacaactttt aaaatcaaaa aatctgggag
      841 aatattttga agcaaaaaac aatgcagaaa agatgaatga gattgaaaac cgtggcttaa
      901 agcaagtaat gaaaaactca gtacgacaga cccaattaaa tagcgaaata aaacaagtga
      961 taaaaaaaga aaaagaggcg gtaagatgtc caaaatgtaa aagtactaat gttggattta
     1021 tgcagcaagg taaaaagaca ttttctgtta aaaaggccgt cgcaggaact cttttaatag
     1081 gtggaacagt tatgggattt ttaggagaaa aagggaagaa gcaatggcac tgtaacgagt
     1141 gcagctgtat atttgagaca aaataaaaaa agccccacgc tctcaaagtt tggcgactct
     1201 gagcgtgagg cgaatctagt atagtaaaaa cctgctttaa gtaggtctct ttactgtact
     1261 cattttaaca aaaaatgagg taaaaaacaa tgagaaaagt agctatttac tctagagtat
     1321 caacaataaa tcaagccgaa gaaggatatt ccattcaagg acagattgaa gctttaacaa
     1381 agtattgtga ggcaatggaa tggaaaattt acaaaaacta ctctgatgca ggtttttcag
     1441 gcggtaaact tgaacgaccg gcaataacag aattgattga agacggtaaa aacaataaat
     1501 ttgatactat tttagtctat aaactagaca ggctatcaag aaatgtaaaa gacacacttt
     1561 acttagtcaa agatgtattt actgctaaca atatccattt tgtcagctta aaagaaaata
     1621 tcgacacttc ttcagcaatg ggaaatctct ttctcactct cttgtcagct attgcagagt
     1681 ttgaaagaga acagattaaa gagcgaatgc agtttggtgt tatgaataga gcaaaatctg
     1741 gaaaaacaac ggcttggaaa acaccacctt atggatatag atacaacaaa gacgaaaaaa
     1801 cattgtcggt caacgagtta gaggctgcta atgtcaggca gatgtttgac atgataatct
     1861 ctggctgctc aatcatgtca attacaaatt acgcgagaga caatttcgtc gggaatacgt
     1921 ggacacatgt aaaagtaaaa agaatattag aaaacgaaac atataaagga ttagtgaagt
     1981 atagggaaca aactttctca ggtgatcatc aagccattat cgacgaaaaa acatataata
     2041 aagcgcaaat agctttagca cataggacag atacgaaaac aaatactaga ccttttcaag
     2101 ggaaatatat gctttctcac atagcaaaat gcggttattg tggtgcccct ttaaaagtat
     2161 gcacaggaag agctaagaac gatggtacga gaaggcaaac ttacgtttgt gttaataaaa
     2221 cagaaagttt ggctagacga agtgttaata attataataa ccaaaagatt tgtaacactg
     2281 gt
//
LOCUS       DATURV010000110         2148 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DATURV010000110 DATURV010000000
VERSION     DATURV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.110"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..>2148)
                     /locus_tag="VCF75_001613"
     CDS             complement(<1..>2148)
                     /locus_tag="VCF75_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEP2547155.1"
                     /translation="DKTILASDAFHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYA
                     LMCYYLGSFFSPLLFFFNLTSMPDAIYLFTLIKFGLIGLAACYSFHRLYPKISAFLMI
                     SISVFYSLMSFLTSQMELNSWLDVFILLPLVVLGLNKLITENKTRTYYLSISLLFIQN
                     YYFGYMIALFCILYALVCLLSLNDFNKMFIAFVRFTAVSICAALTSALVILPTYLDLS
                     TYGENLSPVKQLVTNNAWFLDIPAKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTL
                     ESIPLKIKLANACLLTFIIISFYLQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACE
                     TLSRLKEVTQIKAGFAFIFLIILTSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFS
                     FRNSQIPSTFISAFILIFSLLESGLNTYYQLQGINKEWGFPSRQIYNSQLKDINNLVN
                     SVSKNSQPFFRMERLLPQTGNDSMKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNL
                     NLRYQNNTIIMDSLLGIKYNLSEGPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNV
                     YKDVNLNVNTLDNQTKLLNQLSGKSLTYFNLQPAQLISGANQFNGQISAQASDYQNSV
                     TLNYQINIPKHSQLYVSIPNIIFSNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFAD
                     AKVATFSFVFPKNKQISFKEPHFYSLSIESYLE"
BASE COUNT          820 a          289 c          388 g          651 t
ORIGIN      
        1 cttcaaggta agattcaata gacaaactat aaaaatgagg ttccttaaaa ctaatttgtt
       61 tattttttgg aaaaacaaac gaaaatgtag caactttggc atcggcgaaa tatcctaaat
      121 caaaaaaaga gtaagcgtta tctgtagtat atatgaaatt atgattatct gtctgaatac
      181 gcacctcttt agcatcagga tttgaaaata taatattggg tatgctaaca tagagttgac
      241 tatgtttagg gatgttaatt tgataattaa gggtaacgga gttttgataa tcagaagctt
      301 gtgcagatat ttgtccgtta aattgattag caccagaaat aagttgagct ggttgtaagt
      361 taaaataggt taaagatttc ccacttagtt ggttaagtaa tttggtttgg ttatcaaggg
      421 tattgacatt taggttgaca tctttgtaaa cattacgtgt taatatagct aaagggctac
      481 tatagtgatt ttgataaaga gtagtattcc cgctagtttt tagttttgta aatccaaatt
      541 tatttagagg tccttcgcta agattatatt ttataccaag tagactgtcc ataataatag
      601 tattgttttg gtatctaagg tttaaatttg tgcctttaga ctgaaatccc aatcgatcca
      661 ataaagaact agatagtcta tttcttacag aggaaaattg tgaaatgccg taataattaa
      721 atttcatgct atcgttccct gtttggggaa gtagcctttc cattctaaaa aaaggttgac
      781 tattttttga cacagagttg acaaggttgt taatatcctt taattgacta ttatatatct
      841 gtcgtgatgg gaatccccac tccttattaa ttccttgaag ctggtagtag gtgtttaacc
      901 ctgattcaag aaggctaaag ataagtatga aagcagaaat aaaagtagat gggatttgag
      961 aatttctaaa cgaaaatagt gaaatagtat aacctaataa taaaaaaaca ctaagtaaaa
     1021 aaagagttaa aggtagaaaa ttatattgtt gagaaaagct ataaggaaga gatgtcagta
     1081 taatgaggaa aataaaagca aaacctgctt ttatttgagt cacttctttt agtcgagaga
     1141 gagtctcaca tgcgagtaat aggataacta ttgaaaaaga ccaagcgtag cgatgcaaaa
     1201 acatatttgg tgagtgcatc ccctgccaaa aaagatcaag tggctgtagg taaaaactta
     1261 ttataataaa agttaacaag caggcattgg ctaattttat ttttaaaggg atactttcta
     1321 aagtaaaata aataacacta agcattaggg gaaataatcc tacgtaaatc ataggcagag
     1381 cattaaactt ggtagtatcg tacactccta ttgagagctt agcaggtata tccaaaaacc
     1441 aagcattgtt cgtaactaac tgttttaccg gggatagatt ctctccataa gttgacaaat
     1501 ctagataggt aggaagtatt actagagcac ttgttaaagc agcacatatt gacacagctg
     1561 taaacctaac aaaagcgata aacattttgt taaaatcatt cagacttaaa agacaaacta
     1621 aggcgtaaag aatacaaaaa agagcaatca tgtagccaaa gtagtaattt tgaatgaata
     1681 ataatgatat cgaaagataa taagttctag ttttattttc tgtgataagt ttatttaatc
     1741 caagtacaac aagtggaaga agaatgaaaa catctaacca agaatttagt tccatttgac
     1801 ttgtcaagaa gctcattaag ctataaaaaa ctgagatgga aatcatcaag aaagcactga
     1861 tttttggata taatctatga aaagaatagc atgcagctaa tcctattaac ccaaatttta
     1921 tcaaggtaaa caaatagata gcatctggca tagaggttaa attaaaaaag aaaagtaatg
     1981 gagaaaagaa actgccaaga taataacaca ttaaagcata aaaatttatc cctagtccgc
     2041 ttgtaaaggt ataaaaaaaa ctatcagaac cgtgcatgat gttacgaaag ttctgcgcaa
     2101 aaataacata ctgatgaaaa gcatcactag ctagaattgt tttatcac
//
LOCUS       DATURV010000111         2056 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DATURV010000111 DATURV010000000
VERSION     DATURV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.111"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(108..676)
                     /locus_tag="VCF75_001614"
                     /pseudo
     CDS             complement(108..676)
                     /locus_tag="VCF75_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            993..1209
                     /locus_tag="VCF75_001615"
                     /pseudo
     CDS             993..1209
                     /locus_tag="VCF75_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
     gene            1222..1422
                     /locus_tag="VCF75_001616"
     CDS             1222..1422
                     /locus_tag="VCF75_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEP2547156.1"
                     /translation="MTTMKELTISDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHP
                     VLTGGCVVGFTVGGAYLGYTAN"
     gene            1716..1910
                     /locus_tag="VCF75_001617"
     CDS             1716..1910
                     /locus_tag="VCF75_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEP2547157.1"
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMVGAAQGVIVCAQTGVFIP
                     LQGYLLCGAAGAATNIIWPH"
BASE COUNT          657 a          309 c          417 g          673 t
ORIGIN      
        1 agctatcaag tgtgtcaaaa catactccga atcctttatg aatacgagct ccgaacttaa
       61 tattatagtc ttcgaaaata cttacaagac atcgttctaa ggcttcttca tttggaaaaa
      121 ccaccctcct tcttgctttg tcgcttgatt tctttattga gtgattcaat caagtttgta
      181 gagtaaatac tagaccaaat gtaatgtgga aattgataga aagttaggag attttccgtt
      241 gtttcaagtg atgtcataac ctttttgtaa cgaggcttcc actcagaaac aagtggtgac
      301 catacatgcg ctcaataatg tcactgattt cgcgtgtcgt aacgccagtc tgatagagtt
      361 tgatcaccat ctcttccaag tggtcatctc gtctagcata agatggtaat aaaactggtg
      421 aaaactcgcc gtttcgatct cttgggatga ttaaattgac taagccatac tttgtttcaa
      481 actgacgaga ataagtccca ttacggctat tacctgtgtt gcaaccttct ttttcataag
      541 gttcataacc aagaaaagct gatagttcaa cttgcaagag gtcattcata gctatttcta
      601 aagaggagcg aaaaaattca tcaatatctt gtttttgtgc taggaagtta agtaattctg
      661 tggtaaactg agtcatggga ataaatctct ttctagtaag tttcgtaact ctactataca
      721 ggatttattc ctttttgtgt gtttacacaa gatattttac actaccatca aaaatcttaa
      781 aaggtattta taaaagcatg aatggtatta tttaaacttc ttaattaatt aactattgtc
      841 acagcgtaag tgtagagagg ataaaaagac atttcatgat gaaaaacagc cttttaagat
      901 tttgaaacca tgatactgtt ctttatgata ttctgtacta gtcgaggttg caacgagaca
      961 aatttaagaa ttatgaaaag aaattattcg atatggagat aaagaaactg gaaacatttc
     1021 accaaatgac tatagaaaag cttgccaagg ttgagggtgg taagaataat tggcaggcaa
     1081 atgtcagtgg ggttatcgct gcaggtagtg ctggagctgc tataggtttt ccagtttgcg
     1141 gtgtagcctg gagctaaaac agctattact ctttgggctg gtgtaacagg agctacaggc
     1201 ggattctaga ggaaggaaag gatgacaaca atgaaggaat taacaattag tgacatggct
     1261 agtataagtg gaggaaacgc tccaggagat gctgtaattg gtggtcttgg aggcttagct
     1321 tctggattga aattttgtaa actgccacat ccagtattga caggtggatg tgttgtagga
     1381 tttacagttg gtggagcata tttgggatat acagcaaatt aaatgatcta tacgattttt
     1441 attattatgc atgcgtctat aatgttttta atgattgttg atgccattca taaaaaaatg
     1501 ttgataaatc tataccatat ttagtgtttt taattttcaa tcaattatcc ctttcggaaa
     1561 aatcggtaaa catgttatct atcggagctt tggtagcgtc attatttgtt tttatcgata
     1621 aaagaaaata gtgattatgc caatgaattt agatatcact cttagctttt taaaatacaa
     1681 aagagtgaaa ttaaaatgaa aaagacttca aaaatatgga agtgctaaaa aatggtctgg
     1741 ctatcataaa tggaggcgga aaagacggag caaatatctt tttgacaggg atggtaggtg
     1801 ctgcgcaggg agtgatagtt tgtgctcaga caggcgtatt tatccctttg caaggttatc
     1861 ttttgtgtgg agcagctggt gctgctacaa atattatctg gcctcactaa atatataaat
     1921 gactatctat cactgagtat agtagtagag gagtggtatg aaaactttag atattgttgt
     1981 ttgctataca ataggaatga ttttatcatt aggtcgtctt atttctccta atcactctat
     2041 atttaataaa cttaaa
//
LOCUS       DATURV010000112         1933 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DATURV010000112 DATURV010000000
VERSION     DATURV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.112"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            103..582
                     /gene="rlmH"
                     /locus_tag="VCF75_001618"
     CDS             103..582
                     /gene="rlmH"
                     /locus_tag="VCF75_001618"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEP2547158.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIDLTDERTP
                     DKASDAENQMIMAKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(739..812)
                     /locus_tag="VCF75_001619"
     tRNA            complement(739..812)
                     /locus_tag="VCF75_001619"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Arg,seq:tct)
     gene            977..1740
                     /locus_tag="VCF75_001620"
                     /pseudo
     CDS             977..1740
                     /locus_tag="VCF75_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          686 a          337 c          343 g          567 t
ORIGIN      
        1 actaataaag acctttgtac aaaggttttt attagtagtt gaagaaaaat aacttgtgga
       61 taaattgtgt aaaaagtaat gttcttgtga tacaattaag ctatgaaagt aaaactaatt
      121 tgtgttggaa aactaaaaga aagatacctc aaagatggta tttcagaata tcaaaaaaga
      181 ctttcccgtt tttgtcaatt cgaaatgatt gatttaactg atgaaagaac accagataaa
      241 gcaagtgatg ctgagaacca aatgatcatg gctaaggaag ctcagcgaat ccataaaaaa
      301 attggggaac gtgattttgt aattgcatta gctattgaag gaaaacagtt tccttcagag
      361 acatttagtg aattaatatc aggggttact gttaagggat attcaactat cacgtttatt
      421 attggtggga gtcttggatt agattcaatt attaaaaaaa gagctaatat gctaatgagt
      481 tttggattgt taaccttacc tcatcagtta atgcgtctag tccttactga gcaaatttat
      541 cgtgctttta tgattacaca aggcagtcct tatcataagt aagagagtgt gaactggaat
      601 aactaactct taccacttta ttagataata gtttggtata attaaaagca aatattaaaa
      661 aaagacagac taaatcctat cttttcaata cactaaggta aatacaaaaa aagcactaaa
      721 acagtgcttt tttatctttg atcccagcag gattcgaacc tgcgaccgtt cgcttagaag
      781 gcgaatgctc tatccagctg agctatgaga ccttaaccac atcattttaa caaaaattat
      841 ctaaaactgt caatatttat tttaaaaaaa ctagaaaata acattatatc ccgcgttata
      901 aaatgaagta caacaaaaaa gacatgttcg tctgatatac tagaattccc caattcagta
      961 tggaaagcag atgaacatgt ctaatattaa ttctaccaga aaatcctcct attctcatct
     1021 ttcagccaca gaacggggtg aaattgctgc ttatcttaaa atgggaaaga aacccgttga
     1081 gattgctcga cttctggggc gtcactgttc tacaatctgc cgagaaataa aacgtggttc
     1141 agtagatcag gtaaaggata agaatggaaa acaaaccttc ttcaacgcct atttcgctga
     1201 tagtctatta aaccaatcga tttgtctaag atggttagta tcagaaaacg ttctaagaaa
     1261 gtcatgaaga cgaataagaa gactttaggt aaatctatcg aagaacgtcc agaatatatt
     1321 aatgatcgtt ctgaatttgg gcattgggag attgatttgg ttctcggcaa gaaaaccaaa
     1381 ggtgaagctg ttatgttgac tttggtagag cgccagacac gctatgcact aggagttaag
     1441 ctagaagaca agcagtccca aaccattaac cgtgctgtca cgcatctcat cagtcagtat
     1501 cctattgcat ccatcacagc ggataatggt tctgagttta gtttactctc agacttagaa
     1561 gctgttgacg tttactttgc acatccctat tcttcacatg agagaggaac aaacgagaac
     1621 ttcaatggcc tcctcagaga atatgtccct aaaggaatct cacttaatcc actaacctct
     1681 gaagaacttg acaatgatat tactgccatc aatgagcgcc taagacgact tcttcaatag
     1741 caatcctcaa aattcctgtt tgagctatcc cgaacagctt aacctctgga actctagtta
     1801 tctatgaact cgttgcactt gacttgacaa cttgcggttt aaaaaaaatc aaattttttt
     1861 taaaaaaagt atagacaaaa aaaatcaatc tgctataata gtaagagtgt taaggagata
     1921 tcctccaaaa ctt
//
LOCUS       DATURV010000113         1908 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DATURV010000113 DATURV010000000
VERSION     DATURV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.113"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..622
                     /locus_tag="VCF75_001621"
     CDS             <1..622
                     /locus_tag="VCF75_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547159.1"
                     /translation="SREVSRINNSLQADNDYIKQKYFIEYDIFEKDILARTGLLEIDW
                     ENVSDDIEPLKIHRNFYESISEKTDSEVIEEIKNLEDINFTDEKGNDLDDLTIIVPAQ
                     DEGFKRVFLGQREWYDIRIGKSKRNRIKYIAGYEVAPESGVKYWAEVDKIVPSDNYVG
                     YWKVIFKGEVKEYSEYKKLGNTWPPQNSRYVSKKALDEAKYLSDVF"
     gene            642..>1908
                     /gene="purB"
                     /locus_tag="VCF75_001622"
     CDS             642..>1908
                     /gene="purB"
                     /locus_tag="VCF75_001622"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEP2547160.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDIKVTSCLTQEEIDELFNPIYYTKRVD"
BASE COUNT          614 a          335 c          445 g          514 t
ORIGIN      
        1 gtcacgagaa gtgtcacgca taaataactc cttacaagcc gataatgact acatcaaaca
       61 aaaatatttt atagaatatg atatttttga aaaggatata ttagctagga cagggttatt
      121 agaaattgat tgggaaaacg tttctgatga catagagccc ttaaagatac atcgtaactt
      181 ttatgaatca attagtgaaa aaacagattc tgaagttata gaagagatca aaaatttaga
      241 agatataaat ttcactgatg aaaagggtaa tgatttggat gacttaacca ttattgttcc
      301 tgcgcaagac gaaggattta aaagagtctt tctaggtcaa cgagaatggt atgatatacg
      361 gataggaaaa tcgaaaagga ataggattaa atatatcgca ggatatgaag ttgcaccaga
      421 gagtggtgta aagtactggg ctgaagtgga taaaatagtt ccttcagata actatgttgg
      481 ttactggaaa gtaattttta aaggagaggt gaaggaatat tcagaatata aaaaattagg
      541 aaatacttgg ccccctcaaa acagtagata cgtatctaaa aaggctttag atgaagctaa
      601 gtatttatcg gatgtttttt aattaaagga gaaaaaacaa aatgatttca cgttattcac
      661 gccctgagat ggcggcaatt tggacagagg aaaataaata ccgtgcttgg ttggaggtcg
      721 agattttggc tgacgaggca tgggcagagt tgggtgagat tcctaaggag gatgtggcta
      781 agattcgtga gaaggcggat tttgacattg accgcattct tgaaattgag caggacacgc
      841 gtcacgatgt ggtggctttc acgcgtgcgg tttctgagac gcttggtgag gagcgcaagt
      901 gggtgcacta cggtttgacg tcaactgacg tggtggacac tgcctacggt tacctctaca
      961 agcaagctaa cgacattatc cgtcgcgatc ttgagaattt caccaatatc gtggcagaca
     1021 aggcgcgtga gcacaaaatg accatcatga tgggtcgtac ccacggtgtt cacgccgagc
     1081 caacgacttt cggtcttaag ttggcgactt ggtacagcga gatgaaacgt aatattgagc
     1141 gttttgaaca tgctgccgca ggtgtggaag ctggtaagat ttcaggtgcc gttggtaact
     1201 ttgccaacat cccacctttt gtggaacaat atgtctgtga caaattaggc attcgtccgc
     1261 aagaaatttc aacacaagtt cttccacgtg accttcacgc agaatatttt gcagtgcttg
     1321 caagtattgc aacatctatc gaacgtatgg caacagagat tcgaggtctg caaaagtcag
     1381 aacaacgtga agttgaagaa ttctttgcca aaggtcagaa aggtagctct gctatgcctc
     1441 acaaacgcaa cccaatcggt tcagagaaca tgacagggct agcgcgcgtg attcgtggtc
     1501 acatggtgac agcttatgag aacgtgtcac tttggcatga gcgtgacatt tcgcactcat
     1561 cagctgagcg tatcatcaca cctgacacaa ctatcttgat tgactacatg ctcaaccgct
     1621 ttggcaatat cgttaagaac ttgactgtct tcccagaaaa tatgatgcgc aatatggaat
     1681 caacttttgg tttgatttat agtcaacgtg ttatgctcaa attgattgaa aaaggaatga
     1741 cacgagaaga agcttatgac ttagttcaac ctaagacagc ttattcctgg gacaatcaag
     1801 tggatttcaa accactttta gaagaagaca tcaaagttac ctcttgtctt acacaagaag
     1861 aaattgatga actatttaat ccgatttatt acacaaaacg tgttgatg
//
LOCUS       DATURV010000114         1875 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DATURV010000114 DATURV010000000
VERSION     DATURV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.114"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..452)
                     /locus_tag="VCF75_001623"
     CDS             complement(<1..452)
                     /locus_tag="VCF75_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PriCT-2 domain-containing protein"
                     /protein_id="HEP2547161.1"
                     /translation="MTDDKFDLLPLLDYIDPSKLSYQDWVNVGFALKHEGYTAMDWDI
                     WSQSDSRYKKGECFAKWDSFQGNGLGTVTGATITQLAKENGWTSDYRTSDEAHELDWD
                     STIDRDYKIIDKNWIEAKEIREPTNWSPVHDLITYINTLFESTDKVGYV"
     gene            complement(442..1071)
                     /locus_tag="VCF75_001624"
     CDS             complement(442..1071)
                     /locus_tag="VCF75_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547162.1"
                     /translation="MGEYCHLKELDGLRFGSLTVINRNRNNSKGGNARWNCLCDCGNK
                     TVVIGSKLRSGYTKSCGCARKNDNAKGYSSTRLYRIWKGMMNRCYNHKNDNYKYYGGK
                     GISICDEWLTFINFRTWSLSNGYKESLTIDRINPKGNYTPLNCRWVSMKMQQNNKTNN
                     RYLSYLGQEYTIAEFSEKLNVTYWTVINQLKLGWSVERIVEEARMKNDR"
     gene            complement(1081..>1875)
                     /locus_tag="VCF75_001625"
     CDS             complement(1081..>1875)
                     /locus_tag="VCF75_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2547163.1"
                     /translation="ESKDRAEVLEDFDNDKYNVLCNSMLLTEGWDCPTVDCVVVLRPT
                     KVRALYSQMVGRGTRLAPGKENLLLLDFLWHTERHELCRPAHLIAKTPEVAQKMVDNM
                     TEETEVTFELLEAEEVASKDVVAEREEALAKQLAEMRKRKRALVDPLQFEMSINAEDL
                     VDYVPEFGWEMAPVSDKQKNALEKFGIFTDDIGNAGKATKLLERLAKRRETGLTTPKQ
                     IRMLERYGFKNVGMWKFEVASNMINRIAASGWRLPKGINAREYQPE"
BASE COUNT          502 a          407 c          292 g          674 t
ORIGIN      
        1 acatatccga ctttgtcagt tgattcaaaa agagtgttga tgtaagtaat caaatcgtga
       61 acgggtgacc agttagtagg ttctctaatt tcttttgctt cgatccagtt cttgtcaata
      121 attttatagt cacgatcaat agtgctatcc caatcaagct cgtgggcttc atcactcgta
      181 cggtagtcag atgtccaccc gttttctttc gctaactgtg tgatagttgc gccagtgaca
      241 gtcccaagtc cattaccttg gaaactatcc catttagcga aacattcacc ctttttgtat
      301 ctactgtcag actgtgacca aatgtcccaa tccatagcag tatatccttc gtgttttagg
      361 gcaaaaccaa cattcaccca gtcttgatat gatagttttg atggatcaat ataatcaagt
      421 aaaggtaata aatcaaattt atcatctgtc atttttcatt ctcgcttctt ctactattct
      481 ttctacactc caacctaatt tcaattgatt tattacagtc caataggtaa catttaactt
      541 ctctgaaaat tcagcaatag tatattcttg acctaaatat gataaatacc tattattcgt
      601 tttattgttt tgctgcattt tcatacttac ccatctgcaa tttagtggag tatagttacc
      661 ttttggattt attctatcaa tggttaatga ttctttatat ccattagaga gcgaccatgt
      721 tctaaaatta ataaaagtta gccattcatc acagattgaa ataccttttc caccataata
      781 tttgtaatta tcgtttttgt ggttatagca tctattcatc atgcctttcc aaattctgta
      841 aagtcttgta gatgaataac ctttagcgtt gtcattttta cgtgcacatc cacatgattt
      901 tgtataacca cttcttaatt tacttccaat aacaactgtt ttatttccgc agtcgcaaag
      961 acaattccat cgtgcattgc cacccttaga attgttacgg tttctattaa tgactgtcaa
     1021 tgatccaaat ctaagtccat ctaattcttt taagtgacaa tattctccca tttttgtctc
     1081 ctattcaggt tggtattctc gagcattgat gccttttggt aggcgccatc cgctagcagc
     1141 aatgcggtta atcatgtttg atgctacttc aaatttccac attccaacgt ttttaaatcc
     1201 gtatcgttct agcattctga tttgtttagg tgttgttagg ccagtttctc tcctcttggc
     1261 caatcgctcc aataatttag tagctttacc tgcatttcca atgtcgtcgg taaaaatacc
     1321 aaatttttct aaagcgttct tttgtttatc agaaacagga gccatttccc agccgaattc
     1381 tggtacatag tccactaagt cttcagcatt gatagacatt tcaaattgca acggatcaac
     1441 taatgcacgt ttacgcttac gcatttctgc taactgctta gcgagtgctt cttctcgttc
     1501 tgcgacaaca tctttgctag ctacttcttc agcttctaac aattcaaagg ttacttcagt
     1561 ttcctctgtc atgttatcga ccattttttg agcaacttct ggtgtctttg caattagatg
     1621 agcaggacga caaagttcat ggcgttcggt atgccataaa aaatctaata atagtagatt
     1681 ttctttcccc ggcgctaatc gtgtgccacg accaaccatt tgactataaa gagctcttac
     1741 ttttgtgggt ctcagtacaa ctacgcaatc gactgtcggg caatcccacc cctccgttaa
     1801 aagcattgaa ttgcatagta cattatattt atcattatca aagtcttcta ggacttcagc
     1861 gcggtctttc gattc
//
LOCUS       DATURV010000115         1857 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DATURV010000115 DATURV010000000
VERSION     DATURV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.115"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..589
                     /locus_tag="VCF75_001626"
     CDS             <1..589
                     /locus_tag="VCF75_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEP2547164.1"
                     /translation="KRQFRIKKNLLNTVRKSQLTELQKSINLDPDGFPTVWLAIDLLM
                     FGDIVSILTIMSEKNIKQICQYYSCTPEELVSWLKCLNFIRNVCAHNSNVLDIQITTK
                     PKLRSEWRNYIDTVKIKHNVTKPSNKLSVIIAIVVYLVNTINSKYQWRKIQSSLKALC
                     KEDDSRAKLLGFKDYDSVKAMINGIKCTAFTNSKK"
     gene            774..974
                     /locus_tag="VCF75_001627"
     CDS             774..974
                     /locus_tag="VCF75_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP2547165.1"
                     /translation="MSEEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(1036..1836)
                     /locus_tag="VCF75_001628"
     CDS             complement(1036..1836)
                     /locus_tag="VCF75_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEP2547166.1"
                     /translation="MSKANRRIWQGLAVILIAIITTFTTSTATAAKKIRNFPTTTEIL
                     SGTKATEAPGILPFTGSYKLVLGDLDNLRRPTFAHIQLKDQDEPKIQRKGLKFNPPGW
                     HNYKLTDANGKTTWLMDRGHLVGYQFSGLNDEPKNLVTMTKYLNTGFSDRNPLGMLYY
                     ENRLDSWLALHPNFWLDYKVTPIYHENELVPRQVILQYVGIDEDGNLLQIKLGGEKES
                     VDNYGVTSVTLDNVSPLAELDYQTGMMLDSTQNEEDSNVETEEFEEAA"
BASE COUNT          605 a          303 c          344 g          605 t
ORIGIN      
        1 aaaaagacaa tttaggatta aaaagaattt attgaatact gtaagaaaat ctcaactaac
       61 tgagttacaa aaatcaatta accttgatcc agatggattc ccaacagtgt ggcttgctat
      121 tgatttattg atgtttggtg acatcgtttc aatcttaaca atcatgagcg agaagaatat
      181 taaacagatt tgtcaatatt acagttgtac accagaagaa cttgtttcgt ggcttaaatg
      241 tttgaatttc ataagaaatg tatgtgctca taattcaaat gtactggata ttcaaatcac
      301 aacaaagcct aaacttcgtt cagaatggag aaattatatt gatacagtaa aaattaaaca
      361 taacgtgact aaaccttcta ataagttatc cgttattatt gctattgtag tctatcttgt
      421 aaatactatt aactccaaat atcaatggag aaaaatacag tctagtttga aggctctttg
      481 taaagaagat gacagtaggg caaagctttt aggttttaaa gattatgatt ctgttaaggc
      541 aatgattaat ggaataaagt gcactgcttt tacaaattca aaaaaataga aatttttagc
      601 aagaatcgcc tgacactagc ggctcttgct tttttatttg ttgtgtgtta gtatatattc
      661 ataatcctaa agctttcata aaagctaatt ttccccagtc catgcggctg ggttttttgt
      721 ttgcttatag atgctcaaag tgttaccata gaataaaata aaggaggcat attatgtcag
      781 aagaaaaact aaaagcaaaa gttgaacaag catcaggtag ccttaaagaa ggtgcaggaa
      841 agctaactgg tgataaagag ttagaggcaa aaggatttgt tgaaaaaaca attgccaaag
      901 gtaaagaact agcagatgat gctaaagaag cagttgaagg ggcagtagat gctgtcaaag
      961 aaaaactgaa ataaaaaaac caaccactca gattaatttc tgggcggttg ttttttatag
     1021 tgcagtcaat ttatgttaag cggcttcttc aaactcttcg gtttctacgt tactatcttc
     1081 ttcgttttga gttgaatcta gcatcattcc tgtttggtaa tccaattcag ctaaaggaga
     1141 tacgttgtct agtgtaactg atgttacgcc gtagttatct acactttctt tttcaccacc
     1201 taacttaatt tgaagtagat ttccatcttc atcaattcca acatattgta gaattacttg
     1261 gcgagggact aactcatttt catgataaat gggagtaact ttatagtcta gccagaagtt
     1321 aggatgtaaa gctaaccaac tgtctaatct gttttcgtaa tagagcattc ctaaaggatt
     1381 tctgtcacta aaaccggtat taaggtattt tgtcattgta actagatttt taggttcgtc
     1441 atttaaacca ctaaattggt agccaaccag atggccgcga tccattaacc aagttgtttt
     1501 cccattagca tcagtcagct tgtagttatg ccagcctgga ggattgaatt taagtccttt
     1561 tcgctgaatt ttaggctcat cctgatcttt aagctgtatg tgtgcgaagg ttggtcttcg
     1621 cagattatca aggtcaccca aaactaattt gtagctacca gtaaatggca agattccagg
     1681 tgcttcagtc gccttcgttc ctgacaaaat ctccgtggta gtagggaaat ttctaatttt
     1741 tttggctgcc gtggcagtac ttgttgtgaa agtggttatt atagctatta aaatgacagc
     1801 taaaccttgc caaatacgac gatttgcttt agacatatcc atgtcctcct ttaatta
//
LOCUS       DATURV010000116         1822 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DATURV010000116 DATURV010000000
VERSION     DATURV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.116"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..1800
                     /locus_tag="VCF75_001629"
     CDS             <1..1800
                     /locus_tag="VCF75_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2547167.1"
                     /translation="IDKGIIYKPTKGAYDRTAGQLIQELQKNPNDIGAVLGDYKEEAG
                     AWIRFNPLDGNGVKNDNVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSL
                     HAIVKVDAKDYQEYRKRVDYIYQICKKNGLDIDTQNRNPSRLSRMPGIMRNGHKQFLI
                     DTNIGKTNYEEWYQWIEDLNDDLPEPETLADEWDNLPELAPELIKGVLRQGHKMLIAG
                     PSKAGKSFALIELSIALAEGQKWLGWQCEQGKVLYVNLELDRPSALHRFKDVYDAMGL
                     PPANVANIDIWNLRGKTVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQM
                     AHFTNQFDKVATELGCSVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELE
                     LTEDLIKARSEKAAAKIYQQALQEKALGYYQQEVTLDDLESRYQMQQHFDKAIKDIMV
                     KQPYLEAVKKAQHEVEIATAWRVDGTLREFAKFQPVNMWFSYPKHEVDTTGVLADIQL
                     EDSKPLWKKAKEARKSKEENLKERNQKLETAYSALFDGSTPVTVQEIREYLDLKSNKS
                     VENYIKEHDGYDVKKGVVFQISDNKETEKKENN"
BASE COUNT          638 a          313 c          404 g          467 t
ORIGIN      
        1 atagataagg gcattatcta taaaccaaca aaaggagcct atgacaggac agctggtcag
       61 cttatccaag aattacaaaa aaatcctaat gacattggtg ctgtcctcgg tgactataaa
      121 gaagaagctg gtgcatggat acgatttaac cctttagatg gcaatggtgt caaaaacgac
      181 aatgtaacag actttaggta tgccttggtt gaatccgata gtatggaact tggtaagcag
      241 tatgctttat ttaaagaact agaattgcca atagcgacat tggtccatag cggtaaaaaa
      301 tcattacacg ctattgtcaa agtagatgct aaagattatc aggagtaccg caaacgggtt
      361 gattacatct atcaaatctg taagaaaaac ggccttgata ttgacacaca gaatcgtaat
      421 ccaagtcgtc tatcacgaat gccaggtatt atgcgaaatg gtcacaagca atttttgatt
      481 gatacaaata ttggtaaaac caattatgaa gaatggtatc aatggattga agatttaaat
      541 gatgatttac cagagccaga aacgctagct gatgaatggg ataatttgcc agagctggct
      601 cctgaattaa ttaaaggagt gttacgccaa ggacataaaa tgctaattgc aggaccatca
      661 aaagccggta agtcatttgc attgattgag ttatctattg ctttggctga gggacagaaa
      721 tggttaggtt ggcaatgtga acaagggaaa gtcctctatg tcaatttgga gctagacaga
      781 ccatcagctt tgcaccgctt taaagatgtt tatgatgcga tgggattacc tcccgctaat
      841 gtggcaaaca ttgacatttg gaacctccgt ggaaaaacag taccaatgga taaattagca
      901 cctaaactta tcagacgctc actgaagaaa aattaccaag ctgttattat cgaccctatt
      961 tataaagttc tcactggcga tgaaaacagt gctgatcaga tggcccattt tacaaatcaa
     1021 tttgataagg tggccactga acttggatgt agcgtaattt attgccacca ccattctaaa
     1081 ggaagtcaag gtggcaaaaa atctatggac agggcaagtg gttctggagt atttgctcgt
     1141 gaccctgatg cattgattga cttagtggag cttgagttga ctgaagacct tatcaaggca
     1201 cgctcagaaa aagcagctgc taagatttac caacaagcgt tgcaggaaaa agctctagga
     1261 tactatcaac aggaagtaac gctagacgac ctagaaagtc gttatcagat gcaacaacat
     1321 tttgacaaag ctatcaagga tatcatggtt aaacagccat atcttgaggc cgtcaaaaaa
     1381 gcccagcatg aggtagaaat tgccacagcg tggcgagttg atggaacgct acgtgagttt
     1441 gcgaagtttc agccagttaa tatgtggttt agttatccca agcacgaggt ggatacaact
     1501 ggcgtgttgg ctgatattca gttggaagat agtaaacctc tttggaaaaa agctaaagaa
     1561 gctaggaaaa gtaaagaaga gaatctcaag gaacggaacc aaaaacttga gacagcttat
     1621 agcgccttat ttgatggttc aacaccggtt acagtgcaag aaattcgtga atacttggat
     1681 ttaaaatcca ataaaagcgt tgaaaattat atcaaggaac acgatggtta cgatgtcaaa
     1741 aaaggtgttg tgttccaaat ttctgataac aaagaaacgg aaaagaaaga aaataactag
     1801 aagaattcta aagaaaaatt ac
//
LOCUS       DATURV010000117         1791 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DATURV010000117 DATURV010000000
VERSION     DATURV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.117"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..1250
                     /locus_tag="VCF75_001630"
     CDS             <1..1250
                     /locus_tag="VCF75_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2547168.1"
                     /translation="VWKQLTILALALFFNQFPYLSPLLIDPLLFLVVLRQETKQLFSL
                     KALFLAVAPSVLVDLLSRFMGTIVIPYLFLSSGIYLGHIIFDLLAYLLIFPSFAIINY
                     MIGKDYKMICQSGYSKRSHNFYQTLLIFVLVYYVDIFVILGFTDPFLHFHHSLFVPTP
                     YKLLFLMFILLLVYLLSYFNHSSKEYLKNELRREQQAYMTNLETYGKHLEKLYRDVRA
                     FQSDYLSRIERLGQAIKSESITQIQDIYAQTVHEANDYWDDKHYNISKLRKINISSIK
                     SLLSAKIISAEKSGIDLNVEVPDNIKETYIPELDLLLLMSIFCDNAIEAALEAQQPHM
                     SIAYFLLDDYQMFVVTNTTKKKVDINKIFEEGYSSKGSERGIGLSNAQRILKKYPYLS
                     LRTKSFDKEFSQTLTMPKEEVDR"
     gene            1247..>1791
                     /locus_tag="VCF75_001631"
     CDS             1247..>1791
                     /locus_tag="VCF75_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEP2547169.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLAL"
BASE COUNT          508 a          275 c          321 g          687 t
ORIGIN      
        1 cagtttggaa acaactaacg attttggctt tagccctatt tttcaatcag ttcccgtatt
       61 tgtcgccatt gctaattgat cctctattat ttttagttgt tttaaggcaa gaaaccaagc
      121 aattattttc tttgaaagct ctttttttgg ctgttgcacc tagtgttttg gtggatttgc
      181 tatctcggtt tatgggtaca atcgttatcc cctatctttt tttgtcgagt ggtatttatc
      241 tgggtcatat tatctttgac ttgcttgctt atctgctgat ttttccaagt tttgctatta
      301 ttaattatat gattggaaaa gattacaaga tgatatgcca atccggatat tcgaaacgat
      361 cgcataattt ttatcaaaca ttgctgattt ttgttttggt ttattatgtg gacatttttg
      421 tcattttagg atttacggac ccctttttac attttcatca ttctcttttt gttcccacac
      481 cttataagtt attgtttttg atgtttatac ttcttttagt ttatcttcta tcttatttta
      541 atcatagttc taaagaatac cttaaaaatg agttgagaag agaacaacaa gcttatatga
      601 ctaacttaga gacttatggg aaacatcttg agaaactcta tcgagatgta agagcttttc
      661 aaagtgatta cctgagccgt atagagcgtt taggtcaagc cattaagagt gaatccatta
      721 ctcagattca agatatttat gcccaaacgg ttcatgaagc taacgactac tgggatgata
      781 aacactataa tatctcgaag cttaggaaaa ttaatatctc atcgattaag agcttattgt
      841 cagctaaaat tatcagtgcg gaaaaatcag gtattgattt gaatgtggaa gtgccagata
      901 atataaaaga gacttacatt cctgagttgg acttactatt gttgatgtct attttttgtg
      961 ataacgccat tgaggcagct cttgaagcac agcaacctca tatgtcaatt gcttactttt
     1021 tgttagatga ctatcagatg tttgttgtga caaatacgac taaaaaaaag gtagacatta
     1081 acaagatctt tgaagaaggc tattcttcaa aaggttcaga acgtggtatc ggcctttcta
     1141 atgcgcagcg tattctcaaa aagtatcctt atttatcctt gcggactaaa agttttgata
     1201 aggaatttag ccaaaccttg accatgccaa aggaggaggt ggatagatga tggttatata
     1261 cagtatggct ttattctatg ctaccatctt tattaacagc tgggtcattt ttgcaaaggt
     1321 ttctgctatt aaactaagct ggaaacgggt tagcgttata ggcattagtt ttgtaattgc
     1381 taatatgata tttgacaaag tcattttgat tgaccaactg ttttttatta ttgttagttt
     1441 gttaagtgct ccgaaaaaga aattatttga gcatatgttt aatggtttct ttactatctt
     1501 gattgtcgaa ttactgtttc gcgtcatcgg atctttcttt ttaccagctg tcttaggatt
     1561 tagcattggt caaattaata ataaccttaa gcttttagag ttatgttatc tttttgtatt
     1621 accaatattt tatctgttta gctacatttt cagtattgac ttaagcttaa taaggtttat
     1681 cagtgaagat aagatgaaaa agtgggtatt ttggatgaat actgccatgt tttcttacta
     1741 ctttttcgcc cattttttgg taactgtgca aagcggattt cttgcactat a
//
LOCUS       DATURV010000118         1769 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DATURV010000118 DATURV010000000
VERSION     DATURV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.118"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            295..>1769
                     /locus_tag="VCF75_001632"
     CDS             295..>1769
                     /locus_tag="VCF75_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEP2547170.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDTFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQRLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKASHRKILEDELLENKAAYIVVNRYKNLP"
BASE COUNT          515 a          274 c          308 g          672 t
ORIGIN      
        1 tataaggcta aacctttgca tagatttaga gaattattac agtatgtgaa aatatatcag
       61 aaattgacta aataaaagat tctgttatac ttgttttata atattctttg acgataatat
      121 catttttcaa cttactttgt ttatgcactg tcactttgct atgtgtaata caaagtcatg
      181 ttatttatct catactaaaa aaataatgga taatagctat gttagactag tgacatgaag
      241 aaatattgct tttattgaat cgttagagaa tatgatattt caaggagaaa aatagtgatc
      301 gagttaaacg atgaattcat ccgaaaagag acgatagaac tggccaacga tggcccacga
      361 gtacacacca ctcaatatga aactaaagta cctcggcttc ataaatgcta tctgcttttc
      421 tttagcatta ttatcagttc tttaacaatt gcagtacctt ttttaacaga tgcagccaat
      481 ggtcttcagt cccaaaactt atatatagga atgatgttaa cgaaggggca acttccctat
      541 agtgatactt ttacaacagg agggctcttt tactttgtca tcattgcatt aagttattat
      601 ttgggatcga cactttggct agtctttgtt caggtgtttt gtttttactt atctggttta
      661 tatctttata aactcattaa ttatatgaca ggctttcaaa aagtggcttt aactttttca
      721 attagctact atttgttgtc tgttagtctt ggttttgggg gattgtatcc tactcaactg
      781 gctatgccat ttatattaat atcggcttgg tttttaacta agtattttgc ctgtttagtg
      841 aaagatgagg catttattct ttttggcttt gtaggtgctc ttgcaatgct aattgaccca
      901 agtaccctta tcttttggtc ttttgcttgt gtgacagttt tttcttataa tataagccaa
      961 aagcatcttg caagaggttt ttatcaactg ctagcttcga tttttggaat gattttagtt
     1021 ttttatacag caggatattt cattttgaac ttacaagtgc taaatcctta tttatcacaa
     1081 acgatgattt atccttttac tttttttaaa tcaggaaact tatcgttgct ttttggactg
     1141 gctattcagt tgttcttcgc tttggggctt ggtcttttga cggggatgga gaatgtcatt
     1201 aggcgattta aaaacaattc tgatagggtc gtcaagtggc tatttgtcat ggtcattcta
     1261 gaatctatac ttgtggctat attttcacaa gactatcgcc cctatcatct tttacctctt
     1321 ttaccttttg gattaatttt gactgctatt cctgttggct atcagtatgg tataggatta
     1381 ggtcagagta gtcatcgcag acgtcatggt aaaaatggtg ttggtcgagt aatgatgatt
     1441 tatcttaaga gacactttta tttgccaatt ttaattgtag ggacaatact aatctgttct
     1501 acttattgtt tcattagtag tattcctctt aatcaggagc gtgatcatat tgctagttat
     1561 ttagaacaga gactaaataa aactcaatct atttatgttt gggatgatac ttctaaaatt
     1621 tatttggaca gtaaagctaa atctgtttct caatttagtt ctcctgacat caatacgcaa
     1681 aaagcgagtc atcgaaaaat attagaagat gaactattag aaaataaggc tgcttatatc
     1741 gttgttaacc gctataaaaa cctgcctaa
//
LOCUS       DATURV010000119         1749 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DATURV010000119 DATURV010000000
VERSION     DATURV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.119"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            68..1138
                     /gene="xerS"
                     /locus_tag="VCF75_001633"
     CDS             68..1138
                     /gene="xerS"
                     /locus_tag="VCF75_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEP2547171.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTKAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT          570 a          278 c          321 g          580 t
ORIGIN      
        1 aatacatgag acaatttgcg aaaattcatt tttgaaaaaa ttgttattat taaggaatta
       61 tgatgatatg agacgcgaat tattactaga aaaaattgaa acctataagg ccatcatgcc
      121 ttggtatgtt ttagattatt accaatccaa gttagctgtt ccatacagct ttaccacctt
      181 atatgagtat ttaaaagaat acaaacgctt ttttgattgg ctgatggatg ctgatttaac
      241 gaaggcgcca aagattgctg atattgactt gagcacgctt gagcacctta ccaagaagga
      301 tttagaggcc tttgtgcttt atttgcggga acgaccttct ctcaacacct attccaccaa
      361 agaggggctt tctcaaacca ctattaatcg gaccttgtct gccctatcaa gcctttacaa
      421 gtacttgact gaagaggttg aaaatgacca aggggagcct tatttttatc ggaatgttat
      481 gaaaaaagtg tcaacaaaaa agaaaaaaga aaccctggct tccagagcgg aaaatattaa
      541 gcagaaactg tttttaggtg atgaaactct agctttcttg gattatgtgg ataaggaata
      601 tgagcaaaaa ttgtctaatc gtgccaaatc ttctttccgt aaaaataagg agagggattt
      661 ggctattatt gctctactac ttgcttctgg tgttcgtttg tctgaagcag tgaatcttga
      721 tttaaaagat gtcaatctaa atatgatgat tatcgaagtt attcgcaaag gcggaaaacg
      781 tgattcagtc aatgtagcag gttttgcgaa aggttatttg gagtcttatt tagctgttcg
      841 tcagagacgt tacaaggctg aaaaacagga tcttgctttc tttttaacag aataccgtgg
      901 cgttccaaat cgaatggatg cttctagtat agaaaagatg gttggcaagt actctgagga
      961 ttttaaaatt cgtgtgactc ctcacaaatt gcgtcatacg ctagcgacaa gactatatga
     1021 tgctactaaa tctcaggtgt tagttagtca tcaattaggc cattcctcta ctcaagtcac
     1081 ggatctttat acccatattg tcaatgatga gcaaaaaaat gctttagata acctttaata
     1141 ttacataatt atttttatgt aatattaaag gtaaaaatag cttcaaaatt aactttaata
     1201 gatggctact attaaccgtg acagtttggt gtcaggccgt tttccaaact actcaagcca
     1261 cttagaaaaa ggaatatcac tgctaacttt gtcccttctt tgaatgactt tttttgcgat
     1321 gtttatagat taaatctagt agggttctca tcaaaaagat gagataagcc tgtttttagt
     1381 atgtcagtac ttttgtttag tatgataata cgttattttg taaagtagct tttaggatta
     1441 ttcttgttaa actacttaat gtcattactg ataacttaga gtaatataaa gctaaaactg
     1501 aaggaatgtt agagaatcat gaagacagct tctttaacat caattggttg aaaacaagaa
     1561 tcagataaca ttatcgctag atagactgaa ggaatcaaaa acacagtttg ttacataaat
     1621 aaattcttgt caaaacttgt attttctatt tgaactgata cgattgacaa aggctaaact
     1681 aaaatagctt gactttttta gaatctttgt attttttgtc ggatcccctc ctattttttc
     1741 cttgaattg
//
LOCUS       DATURV010000120         1736 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DATURV010000120 DATURV010000000
VERSION     DATURV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.120"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            112..630
                     /locus_tag="VCF75_001634"
     CDS             112..630
                     /locus_tag="VCF75_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEP2547172.1"
                     /translation="MNEITLSDNLQQIELEISHHKQIAGQSIWEIGRRLKHVKEQNLT
                     HGQFGNWVESIGIAKTEASRFIKIAEEIPNLGTYTNLGTKALYLIATLPDDQKQEQLE
                     RIENGDNPTVRELQEIKRENNHIKHTEAEKATSYLKSLSYGLFTPFETRETPKQKEII
                     ELENKNNGDDAA"
     gene            649..894
                     /locus_tag="VCF75_001635"
     CDS             649..894
                     /locus_tag="VCF75_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547173.1"
                     /translation="MKELESKINELEEMVINMDEVAVVIPWKVAKNLLQRADYLSEEE
                     RRLLQWKFGDSPYQGKRSEKVRNLLEGLRDSGSSDNS"
     gene            complement(853..1062)
                     /locus_tag="VCF75_001636"
                     /pseudo
     CDS             complement(853..1062)
                     /locus_tag="VCF75_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1119..1436
                     /locus_tag="VCF75_001637"
     CDS             1119..1436
                     /locus_tag="VCF75_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547174.1"
                     /translation="MRPKRYPYQKNKLIIQSKNAKTKIELDSGKITINAEEIKVGDLA
                     NEISKVQLAKEKVSETIGQDKKPTETVDFRISRLEKEVEILKEHLKNTRSLAQCAYNL
                     TSR"
     gene            complement(1415..1636)
                     /locus_tag="VCF75_001638"
     CDS             complement(1415..1636)
                     /locus_tag="VCF75_001638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547175.1"
                     /translation="MSKLSPKPTRKKLDRSWKSLDSFLQNVLSDPLFDFALVDLKLYD
                     MDKQDILSELNLIGYDVTDKNNGYLEVRL"
BASE COUNT          678 a          293 c          307 g          458 t
ORIGIN      
        1 agcgatcaca gcaataatta gcgcgatgac gccgataatt gtattaaata attccatttt
       61 tattcttcca atcgttttta ttttaattat accaaattag aaaggataga aatgaatgaa
      121 ataacactat cagataactt gcaacagata gaacttgaaa tcagtcatca taagcagatt
      181 gcaggacagt ccatttggga gattggcaga agattaaagc atgtgaagga gcaaaatctc
      241 actcatggac agtttggaaa ctgggtagag agtattggaa ttgcaaagac agaagctagt
      301 cgtttcatca aaatcgcaga agaaatccca aatttgggaa cgtacacaaa tttgggaact
      361 aaagccctct acctcatcgc cacccttcca gatgaccaaa agcaagaaca acttgaacga
      421 attgaaaatg gcgacaatcc tacagttcga gagttgcaag aaatcaaacg tgaaaacaac
      481 catatcaaac atacagaagc agaaaaagct accagttatt taaaatcact atcttatggt
      541 ttattcacac catttgaaac tcgtgaaaca ccaaaacaaa aagagattat cgaactggaa
      601 aataaaaata acggtgatga tgccgcttag aactagaaag gagaacgcat gaaagaatta
      661 gaaagcaaaa tcaatgaact cgaagaaatg gtaatcaaca tggatgaagt ggctgtagtg
      721 attccatgga aagtagcaaa aaacctacta caaagagcag attatttgtc agaagaggaa
      781 cgcagattgt tgcagtggaa atttggagat tccccctacc aaggcaaacg ttccgaaaaa
      841 gtccgcaact tgctagaggg acttagggat agtggaagtt ccgataatag ctgataattt
      901 taattctgtt gtttggtcat tgtgcataaa aataagagtt tcatttttac gattaggctc
      961 aattcaatga tcaatcacaa agtctccgac aagactatgt aagataacag tttcaaaatc
     1021 atcaatacta tttaatagag gagttaattc ttctaaagtc atttagttac cctccttcct
     1081 aataggataa ctacattata caactagaaa ggaaacacat gagaccaaaa cgttatccat
     1141 atcaaaaaaa taaattaatt atccagtcga aaaacgcaaa gacaaaaatt gaattagata
     1201 gcggaaaaat aactatcaat gctgaagaaa tcaaggttgg tgatttagca aacgaaattt
     1261 caaaagtgca actagccaaa gaaaaagtat ctgaaactat tggtcaagat aaaaagccta
     1321 ctgaaacagt agactttagg atttccagac ttgaaaaaga ggtcgaaatt ttaaaagaac
     1381 acttaaaaaa tacacgttca ttagcgcagt gtgcttataa cctaacttct agatagccat
     1441 tatttttgtc tgtaacatca taaccgatta agttgagttc agataaaata tcttgtttat
     1501 ccatgtcgta aagctttaaa tcaaccaaag caaaatcaaa taatggatca gataagacat
     1561 tttgtaaaaa actatctaaa gatttccatg acctatctag ttttttacga gtcggcttag
     1621 gactcaattt actcataacc ttcccccttc cttgttagat ttaaccaata agaaaagcta
     1681 gtttgagagg ccagcgagag atcttgtttg ttatgagtat attatatcag aaagga
//
LOCUS       DATURV010000121         1720 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DATURV010000121 DATURV010000000
VERSION     DATURV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.121"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            117..758
                     /locus_tag="VCF75_001639"
     CDS             117..758
                     /locus_tag="VCF75_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEP2547176.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWKSIQPNEEVDKVFIVPLRQLLVTDPVYYRLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWRMTAQFTKCLTDILNDESIAKKNPLDIEKSKGR"
     gene            complement(805..1647)
                     /locus_tag="VCF75_001640"
     CDS             complement(805..1647)
                     /locus_tag="VCF75_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEP2547177.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERVISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVIVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKVDWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMTVVLFALGIWLGLWMSQLE
                     EKYK"
BASE COUNT          620 a          306 c          335 g          459 t
ORIGIN      
        1 tataaaaaaa taacatatat atcaaaaatc actaactagg agtaataagt tgaccaaaaa
       61 gctaggattg gttatgatga agataagatt aacggaaaga gaaattaggg aaggctatga
      121 aggacttatt aaaacagtat caggcaaaac cactaggaga ggagaagcgg tacgccgtat
      181 ttttacctct tattcttgtc aatgatgact ggcatgtctt atatgaagtc agaagtcagc
      241 atatttcaca accgggggaa gtttctttcc cagggggaca agtagaaaat caggagacac
      301 ttcaagaagc tgctatccga gaaacagttg aggaattgac tgttgatgcg tcccagatcc
      361 agctatgggg tgaaattgat tatttagtac agtcttctag aactattcat tgtttcgttg
      421 gtcaattagt cgttgatgac tggaaaagca tacagcctaa cgaagaagtt gacaaggtct
      481 ttatagtacc tttacgtcag ctacttgtca ctgatcctgt ctattatcgt ttagaagcca
      541 ctcctattga gacaacggat tttccttttg accgtattcg taatgggaaa tactaccaat
      601 ttagtcaaga gtaccgtagt attccgtttt atgaaaatct agaagaaacc atatggagaa
      661 tgactgccca gtttactaag tgtttaaccg atattctaaa tgatgaatca attgcaaaga
      721 aaaacccctt agatattgaa aaatctaagg ggagatagag cactagtttg ttggcgtcaa
      781 ctttttgtca acaacctgta aaggttattt gtatttttct tctaattgac tcatccaaag
      841 tccgagccaa ataccaagag caaataacac aacggtcata agccaggtta agatacctgc
      901 atgactgtag gaaatggatt cgggactact actatttgga ataagaatta atagcgtgct
      961 ggataataca ataccaatga taaagtggta aacttttgaa tgatggtgct gtaaagcata
     1021 atccatactt ttagaaaaag ccaagatggc taggactcct ccgatagcaa taggtaagaa
     1081 agtccctaat aaatccaaag atttgaatcc tattaacata ggaccataga gtcctaagat
     1141 aagtaagaga tttgacgggc ttagtccagg gaccagtact cctaaagcaa ttaaggcacc
     1201 tgctaaaata aaggttaaaa agttagctgg taaggtaccg attaagtcat ttaagaagta
     1261 aagacctagt cctgaaataa caaaggtaag gactagccat agccagtcaa ctttgtctct
     1321 ttgagactgc ttggttgact ccttaatcag actaggaata gtgccgacaa tagcgccagc
     1381 aaatccccat aagacgataa cttgatagtg cttaagcaaa aactcaacgg ggaaagaaaa
     1441 gagagcaata cctaaaatac caccaattcc tactggtaag aaaaataaga cattttcaat
     1501 aaagttatcc ctcatatgag ctaaaaagga aatcactcgc tcatagattc ctaaaatggc
     1561 tgcgagaaca ccaccggata caccaggtaa aataaaacct agtgcaataa tcatgccttt
     1621 aaaaactctt gaaataaatg aaaccattgt tgcctcctga aaagaatgat tttattgtat
     1681 cataaattta aagaattgct gacacaaagc tgcatatccc
//
LOCUS       DATURV010000122         1643 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DATURV010000122 DATURV010000000
VERSION     DATURV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.122"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            118..1038
                     /locus_tag="VCF75_001641"
     CDS             118..1038
                     /locus_tag="VCF75_001641"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEP2547178.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            1092..1340
                     /locus_tag="VCF75_001642"
     CDS             1092..1340
                     /locus_tag="VCF75_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEP2547179.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1465..>1643)
                     /locus_tag="VCF75_001643"
     CDS             complement(1465..>1643)
                     /locus_tag="VCF75_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2547180.1"
                     /translation="EEKKKIFDRFYRVDKARTRQTGGFGLGLALAQQIVMSLKGNITV
                     KDNDPKGSIFEVKL"
BASE COUNT          577 a          299 c          237 g          530 t
ORIGIN      
        1 aatgtgtggc ttttttgagt taattgtatt aattttcgtg taaatactaa cgaaaaatta
       61 tttatttttt aactaaaatg gtataatgta agcactgtca aatgattagg agctaccatg
      121 tcaaatgaat ttatcaactt tgaaaaaatt tctagagagt cttggaaaac attacatcaa
      181 aaagcaaaag ctctcctcac acaagaggaa cttaaaagta ttactagctt aaatgataac
      241 atcagtatta acgatgtcat tgatatctac cttccattaa ttaatctgat ccaggtttac
      301 aaaattgcgc aagaaaatct ttctttttct aaaagccttt ttctaaaaaa agacattcaa
      361 ttaaggcctt ttattattgg aatttctggt tcagtagctg ttggtaaatc aacaactagt
      421 cgtcttttac aacttttatt atcacgaacg caccccaata gtcaagtcga actggtaacg
      481 actgatggtt ttttatatcc taatcagttt cttattgaac aaggactgtt aaatcgtaaa
      541 ggatttccag aatcttataa catggaactt ctcttagatt ttctagattc catcaaaaat
      601 ggtcaaacgg cttttgcacc cgtctattct catgatattt acgatattat cccaaatcaa
      661 aaacaatcat ttaataaccc tgacttcctt atcgtcgaag gtattaatgt ttttcaaaat
      721 caacaaaaca atcgtttata tatgagtgat tactttgatt tttctattta tattgatgca
      781 gacagcagtc acatcgaaac ctggtacatt gaacggtttc taagcatttt aaaattagca
      841 aaacgtgacc ctcataatta ttatgcccaa tatgcacaat tacctcgatc tgaggctata
      901 gcatttgcgc gcaacgtttg gaaaaccgtt aatttagaaa atcttgaaaa attcattgaa
      961 cctacacgta accgtgctga attaatttta cataaaagtg ctgatcataa aattgatgaa
     1021 atttacctca aaaagtgata aagggttgcc aaacgcatat attttctata taataatgta
     1081 gttagattaa tttggaggtg aaaacattgg caaatattaa atcagctatt aaacgcgctg
     1141 aacttaacgt taaagcaaac gaaaaaaact cagcacaaaa atcagctatg cgtactgcta
     1201 tcaaagcatt tgaagctaac ccatctgaag agcttttccg cgctgcttct tcaagcatcg
     1261 ataaagctga atcaaaaggt ttgattcaca aaaacaaagc aagccgcgat aaagcacgcc
     1321 ttgctgcaaa acttggctaa taaatatcca atgaaaactc cagctggagt ttttttatac
     1381 ggtaaaaact caaaaaatac aataaagcgt tgacagttaa aaataacttt aaaagggctg
     1441 accttattgg tcgccctttt tagcttataa tttgacttca aaaatactac ctttaggatc
     1501 attatccttt actgtaatat ttccttttaa agacatcacg atttgctgag ctaaagccaa
     1561 ccccaagcca aatccacctg tttgccgtgt tctagctttg tcaactcgat aaaaacgatc
     1621 aaaaatcttt ttcttttctt cat
//
LOCUS       DATURV010000123         1601 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DATURV010000123 DATURV010000000
VERSION     DATURV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.123"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(44..349)
                     /locus_tag="VCF75_001644"
     CDS             complement(44..349)
                     /locus_tag="VCF75_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEP2547181.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(361..780)
                     /gene="ruvX"
                     /locus_tag="VCF75_001645"
     CDS             complement(361..780)
                     /gene="ruvX"
                     /locus_tag="VCF75_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEP2547182.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(777..1046)
                     /locus_tag="VCF75_001646"
     CDS             complement(777..1046)
                     /locus_tag="VCF75_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEP2547183.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1160..1558)
                     /gene="spx"
                     /locus_tag="VCF75_001647"
     CDS             complement(1160..1558)
                     /gene="spx"
                     /locus_tag="VCF75_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEP2547184.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
BASE COUNT          476 a          331 c          238 g          556 t
ORIGIN      
        1 aatagccctc acggctactt tttaacatag tgtcactaac aatttagttc tcatctagaa
       61 aactattaaa tacctcttca atcatatccc attcagcatc tgaatcctca ggaatcggtt
      121 gtaaatcccc ttcagtacca tcttcatttt cagtaaatga ataggcttgg atttcaattt
      181 caccagactc atcttcttca gatcccgctg gaaccaagag aacataattt ttaccaaact
      241 cttcacgacc atcaattgtt aataaaattt caaacaaggt ttcatttcct tgttcatcca
      301 caagtgtgat aacttcgtgt tggtggtcgt tttcatgatt atgtgtcatt ttttatctcc
      361 ttaaaaattc cgatttaaat agttctgtaa aatgagttgt gcagctaatt tatcaatgac
      421 tttttttcgc ttaccacgac taatgtcagc ttgctcaata agcatacgct ctgcctcaac
      481 agtcgtcaat cgttcatctt gataatggac tggaagtcca aaaaggtgct ctatgtggtt
      541 tccataagtt atacttgcat caacacgtgg accattggtg ttattcatat tttttggcaa
      601 tccaataaca aactgttcaa cttggtactg cttaacaagc tcttctaaac gtgtaaagcc
      661 aaattctgct ttttcctcat caatcttgat aatttcaagt ccttgggctg taaagccaag
      721 aggatcacta atagctacac caaccgtttt cgagccaaca tcaagtccca ttattctcat
      781 ttaacatcaa ttccatttcc ttgtaaatag tagcgaacaa gttcttctac aatttcatca
      841 cgttcatatt tacgaatctg atttcgagca tcattataac gaggaacata agctgggtcc
      901 ccactcaaga cataaccaac aatttgatta attggattat aacccttttc atcaagtgaa
      961 tgatagactg ctgttaatgt ttcactaatt tgtcgtttgt cgccatcatc caacttaaag
     1021 cggactgttt catctgtaaa tcccatactt acaccttctt tctaaaatta agtgctatgc
     1081 tttattatag cttatttcta ttaaataaac aagaatttca agcgaagaag gtatttaaaa
     1141 aaaagctcta caagagcttt tagagtgcag cacgtaatcg tgcttcagta ttttcaatat
     1201 tacgaataga gcgaggtaaa aaagcacgaa tatcgtcttc tttgtagcct acttgcagac
     1261 gtttatcatc aatcaaaatg ggacttttaa ggatgcgcgg gttatcttga atcaaatcaa
     1321 taacctcact aacacttaat tcttcaatat cgcaatcgag agctttggca taacgatttt
     1381 tagatgaaac aatgctctcc actccatttt cggtttttga taaaattgct aaaatttctt
     1441 ctttagttag cggttctttt cctaagttct gttctttata agcaagctta tgggcattta
     1501 gccaagtttt ggctttcttg cagctcgtac aacttgaaat cgtgtaaatt ttaatcatgt
     1561 tgctgactca ctttctattt cgtgatagct ttattgtagc a
//
LOCUS       DATURV010000124         1547 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DATURV010000124 DATURV010000000
VERSION     DATURV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.124"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            1..623
                     /locus_tag="VCF75_001648"
                     /pseudo
     CDS             1..623
                     /locus_tag="VCF75_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
     gene            629..1318
                     /locus_tag="VCF75_001649"
     CDS             629..1318
                     /locus_tag="VCF75_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEP2547185.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESTILPLLNKVGLGDIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIVLEKEYDGSENLEEL
                     FNNQILFEKRR"
     gene            1324..>1547
                     /locus_tag="VCF75_001650"
     CDS             1324..>1547
                     /locus_tag="VCF75_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HEP2547186.1"
                     /translation="MVQIFLSNLLKIKNTSISKLILIFPIICVMLSLLFSALGGESIL
                     RLTAETTVNQWGVIWINVIIAVNSGLLNKL"
BASE COUNT          553 a          182 c          284 g          528 t
ORIGIN      
        1 gaaaataact tttctttgta tttgtcagta gttcaagatc tattacctca actgattgag
       61 agttgtatta tctctgacat agaagtgagt agctataaac cagaagtaaa tagatatgga
      121 ggacctcatt taataagtta tgcagaggaa attttttgta aagaatctat cttatttctt
      181 aataatacaa tagaattttc agatgatgaa agattagttt gtgctactta cttggttcta
      241 tattatctaa atcacttttt caaagataaa gaaacaaggc gctctttctt atttgagaat
      301 tataccggaa agtataagaa agatttcaaa aatttgccaa ttgatttcct attgagtata
      361 tgaagatttt gaatggagtc acctcagctc ttgatagata tgattttttc caagaaatgg
      421 atagatatct catgtcttat atgaaagagt ataatcgatt tggtggtaca aatgatatct
      481 ataatactaa attcaattta gtaggaagtt ttttacatct atcaatgaat cgattaaacg
      541 ggattgatag agagtttgaa gagaaagtgt attgttttgc ttactataca ctaaacgctc
      601 agcaatatat agaatgggag taactaatat gttgaaaata caaaatctaa aaaaatctta
      661 tggtaagaga accatattga ataatgtcaa tatgaatatt cctaagggaa aagtgtatgc
      721 tttaatcggt ccaaatggtg ctggaaaatc aactattatg aaaattttga caggcttagt
      781 tagtaagaca agtggttcta ttatttttga aggtagagaa tggtcacgtc gggatctgcg
      841 aaaaatcggg agtattattg aagaaccacc actttataaa aatttgagtg cttacgataa
      901 tatgaaggta gttacaacaa tgcttggtgt ttcagaaagc actatacttc cattattaaa
      961 taaagttggt ctaggagata ttgacaagag accagtaaaa caattttctc ttggaatgaa
     1021 gcaacggtta ggtatagcta tctctttaat aaattcacct aaactactta tattagacga
     1081 acctactaat ggcttggacc caattggaat tcaagaatta agggaaatta tagagtcatt
     1141 taaatcagaa ggaatgacaa ttatgatttc aagccatata ctgtcagaag ttgaacatct
     1201 agctgatttt attggattta tctatgaagg aaagattgtt ctggaaaaag aatatgacgg
     1261 ctctgagaat cttgaagagt tattcaataa tcaaatttta tttgaaaaga ggagataggg
     1321 gctatggtac aaattttttt atcaaattta ttaaaaataa aaaatacgag tattagcaag
     1381 ttgattctta ttttccctat tatttgtgtg atgttatctt tattgttttc agcattaggt
     1441 ggtgaaagta tcctacgttt aactgcggaa acaacagtaa atcagtgggg agttatttgg
     1501 attaatgtta ttattgctgt aaattctggt ttgttaaata aattaga
//
LOCUS       DATURV010000125         1536 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DATURV010000125 DATURV010000000
VERSION     DATURV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.125"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            202..813
                     /gene="rpsD"
                     /locus_tag="VCF75_001651"
     CDS             202..813
                     /gene="rpsD"
                     /locus_tag="VCF75_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEP2547187.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(967..1500)
                     /locus_tag="VCF75_001652"
     CDS             complement(967..1500)
                     /locus_tag="VCF75_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP2547188.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFRTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
BASE COUNT          466 a          284 c          272 g          514 t
ORIGIN      
        1 atgatagata gactgacttt acaaaacaac tttcttatgg taaactgttc tttgtgcgaa
       61 ataacagcag aaaaatatga agctcgtcaa caggtggtta tctattcaag taacctttgc
      121 tgtttaggcg aaaaccccct gcacgaatca ggatttttta aaccgttatt tcctcaaaaa
      181 atttagagga ggacatgtaa tatgtcacgt tatactggtc catcatggaa acaatcacgt
      241 cgccttggtt tatcacttac aggcacaggt aaagaattgg cacgtcgtaa ctacgttcct
      301 ggtcaacacg gtccaaacaa ccgtagcaaa ctctcagaat atggtttgca gttagctgag
      361 aaacaaaaac ttcgtttctc atacggttta ggtgaaaaac aattccgtaa cttgttcgtt
      421 caagctacaa aaatcaaaga aggaactctt ggtttcaact ttatggttct tttagaacgt
      481 cgtcttgaca acgttgttta ccgtctaggt ttagcaacta ctcgtcgtca agcacgtcaa
      541 ttcgttaacc acggtcacat ccttgttgac ggtaaacgcg ttgatatccc ttcataccgc
      601 gttgatccag gtcaagtgat ttcagttcgc gaaaaatcaa tgaaagtacc tgcaatcctt
      661 gaagctgttg aagctactct tgggcgtcca gctttcgtat catttgatgc tgaaaaactt
      721 gaaggttcat tgactcgcct tccagaacgc gatgaaatta acccagaaat caatgaagca
      781 cttgtcgttg aattctataa caaaatgctt taattttaag attaaactta caaaaagcct
      841 atttaatagg ctttttttgt atctcataac aaatctattc tgatcccttc attttactat
      901 ggcttctaaa aaagttatca ctaaggctga tgacttgtaa aatgatactt tcgtaagggt
      961 tactatttag gtactcgtaa gcattttgag aacgaattgt gtcatttctt gaggtgattc
     1021 ttttttacct ttagcaatcc aactttgaca gacaccaaaa aaagcatggg ctaagtagat
     1081 actttggtat tctttttccg tttgagataa ctcttcggta cggaacttgt cctgtaaatc
     1141 agtagtgatg agtaggcgaa ctttattgat gataaaggcc tgtatttctt tagtaccgtt
     1201 agcagacaaa agggaagaga gaagctgttc tctttggaga aattcaaata cttcaaggaa
     1261 ggcttgttct ttattttgat attttttctc aaaaatatat tctaatttgt gaaaaaaggt
     1321 ttgctgataa tagtcaatca tttcatattt atctttatag tgtgtgtaaa aactagagcg
     1381 actaattcca gctcttttag ataatttaac tgtggtaata tcgtcaaaac tttcggtttt
     1441 tagtaacata accattgctt ttaaaattgc ttgcttcgta ttttctttac ggttgaccat
     1501 ctaccatctc tccttttgaa cacttttaga cataat
//
LOCUS       DATURV010000126         1533 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DATURV010000126 DATURV010000000
VERSION     DATURV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.126"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..156
                     /locus_tag="VCF75_001653"
     CDS             <1..156
                     /locus_tag="VCF75_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEP2547189.1"
                     /translation="EYNELSKRTKKEKQLAERLKDDNFVKKYARAKYYLSREGEMIYP
                     IPGLLPK"
     gene            291..>1533
                     /locus_tag="VCF75_001654"
     CDS             291..>1533
                     /locus_tag="VCF75_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEP2547190.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPVGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFCSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYE"
BASE COUNT          580 a          234 c          243 g          476 t
ORIGIN      
        1 gagtataatg aattgtcaaa gaggacaaaa aaagaaaaac aattagcaga acgactaaaa
       61 gatgataatt ttgtcaaaaa gtatgctagg gcaaaatact atttatcgcg tgaaggagaa
      121 atgatttatc ctattccagg actattacca aaatgatgat ggacaatatt gtaaaaaaaa
      181 tagaagcatt tcttgctttt tctgataaaa aattagcaga gctgcaacag gaaaatcaaa
      241 aagttaaaga agaaagtcag tatattcaaa aaaactagaa aggagtcaat atgagaaaat
      301 tattagcggc tatgttaatg actttttttc tgactccttt accagtgatt agtacagaaa
      361 aaaaacttat attttcaaaa aatgctgttt atcaattgaa acaagatgtc gttcaatcaa
      421 cacaattcta taatcaacta ccctctaatc caaatcttta tcaagaaacg tgtgcttata
      481 aagacagtga tttaactcta ccagtaggaa gattaggtgt aaatcaacca ttacttatta
      541 aatcgcttgt gcttaacaaa gaatctttac cggtttttga gttagctgat ggtacctatg
      601 ttgaggctaa tcgacaattg atttatgacg atattgtact taatcaagta gatatagata
      661 gctatttttg gacacaaaag aaacttaggc tttattcagc cccttatgtt ttaggtacgc
      721 aaacaattcc ttcttctttt tcatttgctc aaaaagttca tgccactcaa atggcacaaa
      781 caaaccatgg aacttattat cttattgatg ataagggctg ggcatcacaa gaagatctag
      841 ttcaatttga taaccgcatg ttaaaagtcc aggaaatgct cttacaaaaa tataataacc
      901 caaattattc aatttttgta aagcaactca acacacaaac aagtgctggt attaatgctg
      961 ataaaaaaat gtatgctgca agtatctcga agttagcacc actttatatt gttcaaaaac
     1021 aattacaaaa aaagaaatta gcagagaata aaactttgac ttatactaaa gatgttaatc
     1081 atttttatgg agactatgat ccattgggaa gtggtaaaat tagtaaaata gctgataata
     1141 aagattatcg tgttgaagac ctactgaaag ctgtagcaca acaatcggat aatgtagcaa
     1201 ctaatatttt aggttattat ctatgtcatc agtatgataa agctttctgc tcagagataa
     1261 aagctttatc aggtatcgat tgggatatgg agcagcgctt attaacttct cgttcagctg
     1321 caaatatgat ggaagctatt tatcatcaaa aaggccaaat tatttcttac ctttcaaata
     1381 ccgaatttga tcaacaacgt atcacaaaaa atattactgt tccagttgca cataaaattg
     1441 gtgatgctta tgattataaa catgacgttg ctattgttta cggtaatact ccatttattt
     1501 tgtctatttt tacaaataaa tcaacctatg aag
//
LOCUS       DATURV010000127         1433 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DATURV010000127 DATURV010000000
VERSION     DATURV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.127"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..152
                     /locus_tag="VCF75_001655"
     CDS             <1..152
                     /locus_tag="VCF75_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2547191.1"
                     /translation="HSKNRKHEKINLYDAKPNSTRSDVLKKYKDNKTINMKDFSHFDI
                     YLWTK"
     gene            356..667
                     /locus_tag="VCF75_001656"
                     /pseudo
     CDS             356..667
                     /locus_tag="VCF75_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(724..1431)
                     /locus_tag="VCF75_001657"
     CDS             complement(724..1431)
                     /locus_tag="VCF75_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP2547192.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNAYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
BASE COUNT          509 a          255 c          239 g          430 t
ORIGIN      
        1 ttcacagtaa aaataggaaa catgaaaaaa tcaatttata tgacgcaaaa cccaatagca
       61 ccagatctga tgtcttaaaa aaatataaag acaataagac tataaacatg aaagatttca
      121 gccattttga tatttatctt tggactaaat aagctaaaag ctttggataa atatgatcaa
      181 ttgacctata ataacattga taccaaatct aaaactgctc agattgaatt gcgccccaaa
      241 agttagacaa aaaatctaat aattggggtg tttttgtctg gcattaagtt tagctaataa
      301 ccttgcttaa atcacattaa accaataatg gaaaaagaca tctccaaata aatgctatga
      361 tggaactata tgatagagtg atcaaggttg gcaatatcaa cacaactatt atcatcattt
      421 cctaaactct aaaggaattc gtccgtctat gtcacgtaaa agaaacagtc ctgacaatag
      481 catgatggaa tctttctttg gcatcttaaa gtcagaaatg ttctatggtt atgagaataa
      541 ctataaatca ctagaagact tagaataagc gattacaaat gacattgatt attacaacga
      601 taaacatatc aaagtaaaac taaaaggatt tagttctgta caatacagaa ctaaatcctt
      661 tacttaaaat agagtatcca gcttttttgg ggtcagtgca agcagattgt tgttagtgtt
      721 acattaataa gtcatgcgtt tagcccaagc ctcatcaact gctagggtct ccgcatcagt
      781 gtaattacga cgatactcat tccaaacata acccgaagtt gctagaccag tttcttgagc
      841 tgagctatta gcaacatcat aagccgtcaa accataggtt gcaataatat tatttagttt
      901 taagttataa ctggtgtctg tagcgtataa gccagttaga gctgctgtag catcttgata
      961 cgatagcgtg tttgattttc tagcacctat ataagtgcta gaacttagca agtctgcata
     1021 gtcattaagg gagtctgcaa tacttggata agctctaaag gcttgatcaa tcgtataggc
     1081 attgccatta ccatcatctt cccaagttga catcgtcaca gaagaaccgt tataggcacc
     1141 cttaataccg aaaaaattat aataaggtgc ttgacttaga cttgatttac cattacttga
     1201 ttctaaaatc gcttgtgcta tcataacaga cgcataaaga tcacgttctt gaccaatagt
     1261 actagcagtt ggaccaattt catcaataaa atgtcgggtt gaagaactgg cgatagtctc
     1321 agctgacacg tttgaagttt tatgagattg tctcattgca ctataagctc ctaaacaagc
     1381 tgctagaaca aaaagactga taagtacaag cttacctttc ttttttgtca tta
//
LOCUS       DATURV010000128         1356 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DATURV010000128 DATURV010000000
VERSION     DATURV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.128"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            7..>1356
                     /locus_tag="VCF75_001658"
     CDS             7..>1356
                     /locus_tag="VCF75_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP2547193.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLIFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     "
BASE COUNT          398 a          210 c          243 g          505 t
ORIGIN      
        1 gataacatga ctatttttcg taaaaaaaag aaatattcca ataaaacaga aatgcaaagg
       61 cacttcaaag ttattgattt aattttttta ggcctaggtt caatggttgg aacaggtatc
      121 tttacagtta ctggcatagg cgctgctaag tatgcaggtc ctgctttaac aatatcgatt
      181 atcatttccg cgattgctat tggaatttta gccttatttt atgcagaatt tgcgtctcgt
      241 atgccttcta acggtggtgc ttatagttat gtttatgcaa ccttgggaga gtttccagca
      301 tggcttgttg gctggtatat tattatggaa tttttaacag ctatatctag tgttgctgtt
      361 ggctggggaa gttatttaaa aggattactt gccaattata atatagtatt accaaacgct
      421 ttaaatggca cgttcaatct taagaatgga acttacatag atattcttcc tgttttagtg
      481 atgtttttcg tgacagggat tgtcctaatg aattcgaaat tagccctgag attcaatagt
      541 tttttagtta ttttaaaatt ttcagcactg gctttattta tctttgtagg catttttttt
      601 attgatcaca ataattggtc tcattttgct ccatatggtg taggacaaat tactggaggt
      661 aaaacaggta tatttgctgg tgcttctgtt atgttctttg catttttagg atttgaatct
      721 atttcaatgg cagttgatga agtgaaagaa ccacaaaaaa caatccctaa aggcatcatt
      781 ttatctttga ttattgtaac agcactctat atagttgtca ccactatttt aacgggaatt
      841 gtacactaca ctaaattaaa tgttccagac gcggtggctt ttgctttaag aaatattaga
      901 ctttattggg cagctgacta tgtgtcgata gttgctattt tgactcttat tacggtatgt
      961 atttctatga cctatgcatt agctagaaca atttacagta ttagtagaga tggtttatta
     1021 cctaaatcac tttatactct cactaaaaaa aataaagtgc ctcaaaatgc tactcttgtc
     1081 actggactac ttgctatgat ttgtgcggga atatttcctt tatcgagtct agcagagttt
     1141 gttaatattt gtactttagc atatttaatt attctgtcgg gtgcaattat taaactcaga
     1201 agaatagaag gagaaccaaa agcaaatgaa tttaaaactc ctctagtacc attcctacca
     1261 atgttggcaa ttatcatttg tctatcattt atgagccaat ataaagcttt tacttggatt
     1321 gcttttgcta ttgccacaat tattgggaca ttaatc
//
LOCUS       DATURV010000129         1231 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DATURV010000129 DATURV010000000
VERSION     DATURV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.129"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(64..137)
                     /locus_tag="VCF75_001659"
     tRNA            complement(64..137)
                     /locus_tag="VCF75_001659"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Met,seq:cat)
     gene            complement(147..236)
                     /locus_tag="VCF75_001660"
     tRNA            complement(147..236)
                     /locus_tag="VCF75_001660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:tga)
     gene            complement(259..332)
                     /locus_tag="VCF75_001661"
     tRNA            complement(259..332)
                     /locus_tag="VCF75_001661"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Ile,seq:cat)
     gene            complement(353..426)
                     /locus_tag="VCF75_001662"
     tRNA            complement(353..426)
                     /locus_tag="VCF75_001662"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Met,seq:cat)
     gene            complement(432..505)
                     /locus_tag="VCF75_001663"
     tRNA            complement(432..505)
                     /locus_tag="VCF75_001663"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Pro,seq:tgg)
     gene            complement(512..585)
                     /locus_tag="VCF75_001664"
     tRNA            complement(512..585)
                     /locus_tag="VCF75_001664"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(549..551),aa:Arg,seq:acg)
     gene            complement(602..686)
                     /locus_tag="VCF75_001665"
     tRNA            complement(602..686)
                     /locus_tag="VCF75_001665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(650..652),aa:Leu,seq:taa)
     gene            complement(695..766)
                     /locus_tag="VCF75_001666"
     tRNA            complement(695..766)
                     /locus_tag="VCF75_001666"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:Gly,seq:gcc)
     gene            complement(779..851)
                     /locus_tag="VCF75_001667"
     tRNA            complement(779..851)
                     /locus_tag="VCF75_001667"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(816..818),aa:Thr,seq:tgt)
     gene            complement(862..943)
                     /locus_tag="VCF75_001668"
     tRNA            complement(862..943)
                     /locus_tag="VCF75_001668"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(907..909),aa:Leu,seq:tag)
     gene            complement(949..1021)
                     /locus_tag="VCF75_001669"
     tRNA            complement(949..1021)
                     /locus_tag="VCF75_001669"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(986..988),aa:Lys,seq:ttt)
     gene            complement(1053..1125)
                     /locus_tag="VCF75_001670"
     tRNA            complement(1053..1125)
                     /locus_tag="VCF75_001670"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1090..1092),aa:Asp,seq:gtc)
     gene            complement(1143..1215)
                     /locus_tag="VCF75_001671"
     tRNA            complement(1143..1215)
                     /locus_tag="VCF75_001671"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1180..1182),aa:Val,seq:tac)
BASE COUNT          324 a          352 c          273 g          282 t
ORIGIN      
        1 atggagcttc aatccattgc tctaccaact gagctaccga gccaacaact acatacttat
       61 gtattgcggg agcaggattt gaacctacga ccttcgggtt atgagcccga cgagctacct
      121 aactgctcca tcccgcgata gttaaaagga ggatgtggga ttcgaaccca cgcacgcttt
      181 tacacgcctg acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccaaaa
      241 taatgattat ctatgatatg gaccttgtag gactcgaacc tacgaccgct cggttatgag
      301 ccgagtgctc taaccagttg agctaaaggt cccaagtcat ccataaaaat aatagcggcg
      361 aaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca gctgagctac
      421 accgccatta atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta
      481 ctctaccaag ctgagctact tcccgaactg atgcacccta gaggagtcga acctctaacc
      541 gcctgattcg tagtcaggta ctctatccag ttgagctaag ggtgctattt caaaaaatta
      601 atgccgagga ccggaatcga accggtacga tgtaaaccat cgcaggattt taagtcctgt
      661 gcgtctgcca gttccgccac cccggctcct caaaagcgaa cgacgggatt cgaacccgcg
      721 acccccacct tggcaaggtg atgttctacc actgaactac gttcgcaact aatctcaatg
      781 ccggctacat gacttgaaca cgcgaccctc tgattacaaa tcagatgctc taccaactga
      841 gctaagccgg ctaatctttt atgcgggtta agggacttga acccccacgc cgttaagcgc
      901 cagatcctaa atctggtgcg tctgccaatt ccgccaaacc cgcttttatg acccgtactg
      961 ggctcgaacc agtgacccat tgattaaaag tcaattgctc taccaactga gctaacgagt
     1021 ctttccttca aaaaatatga ctactctttg aacggtcccg acgggaatcg aacccgcgat
     1081 cttcgccgtg acagggcgac gtgataaccg ctacactacg ggacctgtat tttccaatac
     1141 tatgggagtt aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag
     1201 ctgagctaaa ctcccattga gtgtgtcatt a
//
LOCUS       DATURV010000130         1203 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DATURV010000130 DATURV010000000
VERSION     DATURV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.130"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            152..>1203
                     /locus_tag="VCF75_001672"
     CDS             152..>1203
                     /locus_tag="VCF75_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP2547194.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQVDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSM"
BASE COUNT          419 a          186 c          197 g          401 t
ORIGIN      
        1 tattactggc cttattttgc agtaaatcat aggagcaagt caattgcttc tttttttgtt
       61 taaccgtaag gaatttaaaa agttatcact cacctagcct gaataaaaat ggtataatcg
      121 taaggatcac actatttatt aaggaaacat tatgaaaatt gttcagggag tacaacttca
      181 tctgattaag accaaacagt ttaaaaccaa tcatattacc tttcgttttt caggtgattt
      241 gaaccaaaaa acagtagcaa aaagagtctt agtggcacag atgttagcaa cagctaatga
      301 gtgttatcca actgttaggc agtttagaga aaaattagca cgactttatg gagctagctt
      361 gtcaactaac gtgttaacaa aagggttagt tcatattgtg gatatcgata ttacttttat
      421 tcaagataga tatgcttgca atggggaaaa aatactagat gaaatgattc agtttttaaa
      481 agatatcttg ttctcacccc tattatccat cgcccagtat cagcctaaag tttttgagac
      541 tgaaaaaaat aatttgatta actacatcga gtcagataga gaggattctt tttactacag
      601 ctctcttaag gtaaaagaat tgttttattg taataagaat ctacaaatgt cagaatatgg
      661 tagcccagaa ctaattgcaa aagagactgc ctatactagc tatcaagaat ttcataaaat
      721 gctcaatgaa gatcaggttg atattttcat tttaggagat tttgatgatt atcgcgttgt
      781 tcagctaatt catcaatttc cacttgataa ccgaaataaa aacctaaatt tctttcattt
      841 acaaaattct gtcaatatta ttaaagaatc tattgaaaaa agagcagttc atcaatctat
      901 tttacaatta gcttatcact tcccttcagt ttttggacaa cgtgactatt atgctttagt
      961 tcttttaaat gggttgttag gctcttttgc acattcccgt ctctttataa aaattagaga
     1021 agaagaagga cttgcttata gtatagggtg tcgttttgat agttatacag gtttatttga
     1081 aatttatact ggaattgaca gtcaacatcg cactaaaacg ttacaactaa tcatccaaga
     1141 attaaatgcc attaaaatgg gacgattctc agaacaattg attaaaaaga ccagatcgat
     1201 gtt
//
LOCUS       DATURV010000131         1185 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DATURV010000131 DATURV010000000
VERSION     DATURV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.131"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..1006
                     /locus_tag="VCF75_001673"
     CDS             <1..1006
                     /locus_tag="VCF75_001673"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEP2547195.1"
                     /translation="TSAAVLAAVTLFALSVDRGVIADSFSANQEIRYSEVTPYHVTSV
                     WTKGVTPTANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNMLHWWLDQ
                     NNEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKAFPGLSAR
                     RIGVMPDLVLDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGDQSKLLTS
                     RHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGNLKAIY
                     VTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDSWNQTN
                     "
     gene            1026..>1185
                     /locus_tag="VCF75_001674"
     CDS             1026..>1185
                     /locus_tag="VCF75_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547196.1"
                     /translation="MSSTDDGILRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEF
                     DFKIRKFLM"
BASE COUNT          414 a          191 c          232 g          348 t
ORIGIN      
        1 aacttcagct gcagtattgg cagcagtgac tttatttgct ctatcggtag atcgtggtgt
       61 tatagcagat agtttttctg ctaatcaaga gattagatat tcggaagtaa caccttatca
      121 cgttacttct gtttggacca aaggagttac tcctacagca aacttcactc aaggcgaaga
      181 tgtttttcac gctccttatg ttgctaacca aggatggtat gatattacca aaacattcaa
      241 tggaaaagac gatcttcttt gcggggctgc cacagcaggg aatatgcttc actggtggtt
      301 agatcaaaac aacgaaaaaa ttgaagcata tctaaaaaaa cacccagata aacaaaaaat
      361 catgtttggt gatcaagaat tattggatgt aagaaaagtt attaatacta aaggtgacca
      421 aacaaatagc gagcttttta attatttccg agataaagct ttccccggtt tgtcagcacg
      481 ccgaattgga gttatgcctg atcttgtttt agatatgttt atcaatggtt attacttaaa
      541 tgtttataag acacagacta ctgatgtcaa tagaacctat caagagaaag atcgccgagg
      601 tggtattttt gacgccgtat ttacaagagg tgatcaaagt aagctattga caagtcgtca
      661 tgattttaaa gaaaaaaatc tcaaagaaat cagtgatctc attaagaaag agttaaccga
      721 aggcaaggct ctaggcctat cacacaccta cgctaacgta cgcatcaacc atgttataaa
      781 cctgtgggga gctgacttcg attctaacgg gaaccttaaa gctatttatg taacagactc
      841 tgatagtaat gcatctattg gtatgaagaa atactttgtt ggtgttaatt ccgctggaaa
      901 agtagctatt tctgctaaag aaataaaaga agataatatt ggtgctcaag tactagggtt
      961 atttacactt tcaacagggc aagatagttg gaatcagacc aattaaagcg tatcaataag
     1021 aaagaatatc ttcaacagat gatggtattc taaggactaa tactattgca gggaattttt
     1081 ttatagatgg gcaacagcaa aaaacattaa taaatcctat aaaaatcgat aaggatattg
     1141 ttacgattca agagttcgat tttaaaataa gaaaattttt gatgg
//
LOCUS       DATURV010000132         1183 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DATURV010000132 DATURV010000000
VERSION     DATURV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.132"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(61..1182)
                     /gene="mnmA"
                     /locus_tag="VCF75_001675"
     CDS             complement(61..1182)
                     /gene="mnmA"
                     /locus_tag="VCF75_001675"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEP2547197.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDEDGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVAVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          351 a          278 c          203 g          351 t
ORIGIN      
        1 tcatatgatg tcaaaggaat tgttgaatgt ccctttcctt ttatcttaat aaaagattca
       61 ttaaatgtac tgacaaggct gcccattttt ataagccatg tcaatcatac caccaccaag
      121 gcattctttt ccgtcataga aaacaacagc ttgaccagga gtaatcgcgc gctgtggctc
      181 tgcaaaaaca acttctgcct tatctcctcg aacatgcacc gccacgtgtg aatcgggttg
      241 acgatagcga aatttagcag tacattcaaa tgtgaactct tctggcatct cacgtgtaaa
      301 atggatgact gaggcgtcaa gactatttga cattagggct tcatggtaga aaccttgacc
      361 aacgtataaa atattttgcg acaaatcttt accgacaaca aaccaaggtt gattatcccc
      421 accatgctga ccaccaatgc ccagaccacc acgctgacca atagtataat acataagacc
      481 ggcatgttct cccatatcgc gaccatcaat agtcatcatg cgaccttttt gtgccggtaa
      541 gtactggctc aaaaactgtt tgaaattctt ttcaccaata aaacaaatac ctgttgaatc
      601 tttcttttta gccgttgcaa gccctgctcg ctcagcgatt tctctaactt ctgacttttg
      661 caaatgacca agggggaata aagtcttttg tagttgttcc tgtgatagtt gacttaaaaa
      721 gtaggtttgg tctttgccat tatcagctcc acgtaacatg tgaaccgtac catcttcatc
      781 acgcttgact tgagcatagt gtcccgttgc tacataatct gcacctaaag tcatggcata
      841 gtcaaggaaa gctttaaatt ttatttcctt attacacatg acatcgggat ttggagtacg
      901 acctgctcgg tattcggcca agaaatattc aaatactctg tcccaatatt ctttttcaaa
      961 attgactgaa tagtaaggaa taccgatttg atcagcaacg gctgctacat ctttgtaatc
     1021 ttcagtagct gtgcaaacac caaattcatc tgtatcgtcc cagtttttca tgaagacacc
     1081 aataacatca taaccttgct cctttaaaag tagagctgtt acagatgaat caacgccacc
     1141 actcatacca acgacaacac gtatctttga gttatctgtc ata
//
LOCUS       DATURV010000133         1136 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DATURV010000133 DATURV010000000
VERSION     DATURV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.133"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..936
                     /locus_tag="VCF75_001676"
     CDS             <1..936
                     /locus_tag="VCF75_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2547198.1"
                     /translation="AQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSDKVL
                     QGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQLNY
                     DVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSNLSR
                     FRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFGLLI
                     GAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIAIFQ
                     SGGTLSQAWPYLLALMGTALALIIFSSLLLKPTRL"
BASE COUNT          348 a          187 c          199 g          402 t
ORIGIN      
        1 gctcaactaa agcaaaacaa aaagtacgac atttttacca aaatcaaaaa agagcatatt
       61 gatcactacc ttcaagataa atctctagaa gcggtattga ctattgataa agggttctca
      121 gataaagtgt tgcaaggaaa atctcaaaaa ctcaatatac gttcgattgc taatagcgaa
      181 attacagagt gggtaaaagc acagaccaat tatctattgg aaaactataa tattataggg
      241 gatgtggctc taggtaacga agatactttt aatagaattc tccaaaaaaa ccaacagtta
      301 aattatgatg ttaagcaagt aactttgaca gaccgatcac gtagcaaagc agtatcttcc
      361 acaacgacag gatttttgct tattctaatg ttaggtagta cgagcgtgat ttacagtggt
      421 attttggcag ataaatctag tcagttatac catcgattaa tgttatcgaa cttatctcgt
      481 tttcgctata tgttgagtta cgtctgtgta ggatttgttg cttttacgat tcaaattgtc
      541 attatgctga gtttgttaaa agtatttaat attagttttt ttgttccgac atcacttctt
      601 ttgattattt ttttcctttt tagtttatta gcgataggct ttggcctgct gattggagct
      661 atcacacaaa attcccaaca aagtagccag ttagctaatc ttattgtaat gccgacttca
      721 atgttggctg gctgtttatg gccattgtct attacgcctt cttacatgca agcaattggt
      781 aagttattac cacaaaattg ggttttatca gccatagcta tttttcaaag tggagggacc
      841 ttatctcagg cttggccata cttgttagct ctaatgggaa cagcactagc tttgattatt
      901 ttttcaagtt tattattaaa acctaccaga ctataattag tcttttgtta aaaaatactt
      961 tcctaattag ctaactacca ggtagccctt ttattggctt atatgcaata aaagggttgc
     1021 tttttgctta tattttctct agtgcactta tcttttgtta aaagcgattg cgatgttact
     1081 gttgttactg tgttttttag agagcatatt atcaaaagcg acctctctag aaagtt
//
LOCUS       DATURV010000134         1087 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DATURV010000134 DATURV010000000
VERSION     DATURV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.134"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(364..645)
                     /locus_tag="VCF75_001677"
     CDS             complement(364..645)
                     /locus_tag="VCF75_001677"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP2547199.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
BASE COUNT          364 a          121 c          219 g          383 t
ORIGIN      
        1 agaatattta aatcgttcat tatttcgata actaaaatga taggctcttt gactactgta
       61 gaggagtaaa acgtttaact tgatggcttt gataaggttg ttgactgttt ctaatttggc
      121 cttagagtag ttgagtttaa gtatattgct aatcttgttt tcttttcttt tcttttagaa
      181 aaattttaaa aattgtctga aaaatgggaa gagcaagagt tgatagagtt aggaataatt
      241 attgaaattc agagatcttg tgagtaagag cttttctttt ctagatgtta gttatgggcg
      301 tacgaaaaat tgggcaataa agctattatt gatgagcttc cgtgaatcat gctgtatgag
      361 ttttcagtaa agtttgagag ttctatacgc gataggtttt atagcagaag tatttatgat
      421 tcggatacga aagcgagtta tagcttgtct aagacgttgg gggatatgaa aacaatcgag
      481 gataatttta gcctgtagaa aaagcatagt aatctcatag tagtgggcac tgagtatgcc
      541 tatggtaata actttgacat gccttctcac ctgtctggta taatgaaaga gatgattatg
      601 aatgcttttt gcgtccccag catttacata atttaaaacc gtgatgctaa ggttttagat
      661 tcctaatgaa attatactat aatcggatta ttctatatga gtctttctga aatgatagtt
      721 tggtcgcttt tcattataga tcgtcatatg gaacttttta catatgttta aaaagactct
      781 ataatctttg aggtggattt acctactaca gaaagtatag aattaaaagt tgtgaaagtt
      841 tgcgagtctt tatgaattgt gattgagtga tggaatacta agatttatag atgaccagtt
      901 ttcgatagtt agcttataaa aaaacaaggt ctgactatac ttatattaat aatgataagg
      961 tcaaaaaaga tgtctaatat tctaattgga aaatggaaag gcataaactg ataatagaaa
     1021 atgtcactat tgtggataat attttaggcg aaaagaagtt attaacctgt ggataagtaa
     1081 ctaaaaa
//
LOCUS       DATURV010000135         1082 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DATURV010000135 DATURV010000000
VERSION     DATURV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.135"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..>1082
                     /locus_tag="VCF75_001678"
     CDS             <1..>1082
                     /locus_tag="VCF75_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP2547200.1"
                     /translation="ENGLTVYFIKKIGFLEKTAMLTVGFGSLDNKLTVDDESRDAPAG
                     IAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQTSFFFSTASKFQENLELLQYF
                     VLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGILQNLFPKTSLANDIAGSKASI
                     QKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLAIQRFQTTLSYPDRKRVTVDPL
                     HYYPVIKSSSVDMDVTTAKLVVGFRGYLTLTQHSLLTYRIALKLFLSMLIGWTSKIYH
                     TLYEDGKIDDSFDVDVEIHHHFQFVLISLDTPEPIAMSNYIRQKLATIKISKEFTNEH
                     LNLLKKEMYGDFIQSLDSIEHLTHQF"
BASE COUNT          369 a          180 c          175 g          358 t
ORIGIN      
        1 tagaaaatgg tttaactgtt tattttataa aaaaaatagg gtttttagaa aaaacagcta
       61 tgttgacagt aggtttcggt tctcttgaca ataagttgac agtcgatgac gaatcacgag
      121 atgctccagc aggtattgca cactttttag aacataaact ctttgaagat gaatcaggtg
      181 gagatatttc attaaagttt acacagctag gagcagagac aaatgccttt acaactttca
      241 accaaacgag tttcttcttt tcaacagcta gtaaatttca ggaaaatttg gaactccttc
      301 aatattttgt tctttctgct aatataacag atgaatctgt tagtagagaa aaaaaaataa
      361 tcgggcaaga aattgatatg tatcaagatg atgctgacta ccgtgcatat agtggaatcc
      421 ttcagaactt atttccaaaa acaagtttag ctaatgatat tgctggctca aaagcatcca
      481 ttcaaaagat cacgaaaata ttattggaaa cgcatcatac ttatttttac caacctacta
      541 atatgagtct attcattgtc ggtgatattg atattgacga aactttttta gctattcaaa
      601 ggtttcaaac cacattatcc tatccagata gaaaaagggt aactgttgat cctttacact
      661 actatcctgt cattaaatca tcttctgttg acatggatgt tactactgcc aagctggttg
      721 ttggttttcg agggtatctg accttaactc agcattcact attaacgtat cgtattgcct
      781 tgaagctatt tttatcaatg cttataggtt ggacttctaa aatctatcat acgctatatg
      841 aagatggtaa aatagacgat tcatttgatg tggatgttga aattcatcat cattttcaat
      901 ttgtattaat atcacttgat acaccagagc ctatcgcaat gtctaactat atccgtcaaa
      961 aattagccac tataaagata tcaaaagagt ttacaaatga gcacttaaat ctattaaaaa
     1021 aagaaatgta tggtgacttt attcagagtt tagactctat agaacattta acccatcagt
     1081 tc
//
LOCUS       DATURV010000136         1037 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DATURV010000136 DATURV010000000
VERSION     DATURV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.136"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            32..>1037
                     /locus_tag="VCF75_001679"
     CDS             32..>1037
                     /locus_tag="VCF75_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEP2547201.1"
                     /translation="MKITVVGIGYVGLSIGLLLAKEHDVTFFDIDNKKIDLINKRQSP
                     LKEAAINKLLCKAKNINATSSEELAYKDATFIILSLPTNLKFNKLDTSIIEISVSNIL
                     KINKKATIVIKSTVPIGFTEYLRNQFHYNDIIFSPEFLREGSTIHDQLYPSRTIVGNE
                     SRNSQLFLDILTDISVEKDSPSLLVGSSEAEAIKLFSNAYLAQKIAFFNELDTFAEMQ
                     NLDSKKIIEAMGYDQRIGNSHNNPSFGFGGYCLPKDIKQLEYHFKEIPAPIITSISES
                     NLLRKIHIAKTILNSSAKTIGIYRINSKKDSDNCRESSTIDVAKLLKSSGKDVIIFEP
                     L"
BASE COUNT          382 a          153 c          169 g          333 t
ORIGIN      
        1 atttataaaa agttttagaa tagaggttaa catgaaaatt acagttgtag gcattggata
       61 cgttggatta tcgatagggc tcctacttgc aaaggaacac gacgtcacct tttttgatat
      121 tgataataaa aaaattgatt taataaataa aaggcaatcc ccccttaaag aagcagctat
      181 aaacaaactt ttatgtaagg caaaaaatat taatgcaact tcttctgaag aattagcata
      241 taaggatgcg actttcataa tcttgtcttt gccaaccaac ctaaaattta ataagcttga
      301 tacttccatt atcgaaattt ctgtaagtaa tattttaaag ataaacaaaa aggctacaat
      361 tgtaataaag tcgacagttc caattggttt tacagaatat ttaaggaatc aatttcacta
      421 caacgatatc attttttcac ctgagttcct tagggaagga tcaactattc atgatcaatt
      481 gtatccttcg agaactatag ttggaaatga atctagaaat tctcaattat tcttagacat
      541 actaacagat atatcggttg aaaaagactc gccatcttta ttagttggct cttctgaagc
      601 agaagcgata aagttatttt cgaatgcata cttggcacaa aaaattgctt tttttaatga
      661 gttggatacg tttgctgaaa tgcaaaattt ggactcaaaa aaaattattg aggctatggg
      721 atatgaccag agaataggaa attcgcacaa taatccttct ttcggttttg gtgggtactg
      781 tcttcctaag gatattaagc aattagagta tcattttaaa gaaattccag caccaattat
      841 taccagtata agtgagtcta atttattaag aaaaattcat atagcaaaaa cgattttgaa
      901 cagctcagct aaaacaatag gaatttatag aattaattcc aaaaaagatt cggataattg
      961 tagggaatct tctacaattg atgttgctaa acttctaaaa agcagtggta aggatgttat
     1021 catctttgag cccttaa
//
LOCUS       DATURV010000137         1002 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DATURV010000137 DATURV010000000
VERSION     DATURV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.137"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            10..>1002
                     /locus_tag="VCF75_001680"
     CDS             10..>1002
                     /locus_tag="VCF75_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEP2547202.1"
                     /translation="MLGEAFLLIIARFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVW
                     YLALTFAKIQKNISLYDYQALTRRKWWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNAS
                     IIVLTAIIMFSISGIGYRWFSAILAMITGLSTVFLGTIAVIGVERVAKIPVFGYVAKR
                     FSAFFNPFHDLTDSGHQLANSYYAMSNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVI
                     EELGLIGAGFILALVFFLILRIMNVGIKAKNPFNAMMALGVGGMMLMQVFVNIGGISG
                     LIPSTGVTFPFLSQGGNSLLVLSVAVGFVLNIDASEKRDDIFKEAELSYRKDTRK"
BASE COUNT          247 a          172 c          224 g          359 t
ORIGIN      
        1 actgtggtta tgttaggaga ggcctttctt ttaattattg cccgcttctt tacaactgct
       61 atcaaaggag cgcatggttg gattgtgatc ggaccagtca gctttcagcc tgctgagtat
      121 ttaaagatca ttatggtatg gtatttggca ctgacctttg ctaagatcca aaaaaacatt
      181 agcctttatg attatcaagc actaactcgt cgaaaatggt ggccaacaca atggaacgat
      241 ttacgggact ggcgtgttta ttctttgttg atggtacttt tggttgctgc tcagcctgac
      301 ttaggaaatg cttccattat tgtattgaca gccattatta tgttttctat cagtggcatt
      361 ggttaccgat ggttttcggc tatcctagcc atgattaccg gtctatccac ggtctttctt
      421 gggactattg ccgttattgg ggtagaaaga gttgctaaga ttcctgtttt tggctatgtc
      481 gctaaacgtt ttagtgcctt ttttaatcct tttcatgact tgaccgattc aggtcaccaa
      541 ttagccaatt cttactatgc tatgagtaat ggtgggtggt ttggccaagg gttaggaaat
      601 tccattgaaa agcgtggcta tttgcccgaa gcacagacag actttgtctt ttcagttgtc
      661 attgaagagt taggtctaat aggagcaggg tttattttgg cacttgtttt cttcttaatt
      721 ttacgtatta tgaatgttgg tattaaagca aaaaatcctt ttaatgccat gatggcttta
      781 ggagtaggcg gtatgatgct catgcaagtc tttgttaata ttggtggtat ctcaggctta
      841 attccatcta ctggtgttac cttccctttt ttgtctcaag gtggaaatag cttacttgtc
      901 ttatctgttg cagtgggttt tgtactaaat attgatgcta gcgaaaagcg agatgatata
      961 tttaaagaag ctgaattatc ttatcgaaag gacactcgaa aa
//
LOCUS       DATURV010000138          919 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DATURV010000138 DATURV010000000
VERSION     DATURV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.138"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..268
                     /locus_tag="VCF75_001681"
     CDS             <1..268
                     /locus_tag="VCF75_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEP2547203.1"
                     /translation="TDYNSKTKGSLIFTLPYDKGWSAQKDGKNLPVKKAQGGFLSVTI
                     PKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSKLL"
     gene            complement(766..839)
                     /locus_tag="VCF75_001682"
     tRNA            complement(766..839)
                     /locus_tag="VCF75_001682"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(803..805),aa:Asn,seq:gtt)
BASE COUNT          329 a          149 c          140 g          301 t
ORIGIN      
        1 cactgattat aattcaaaaa cgaaaggttc tcttattttt acacttcctt acgataaagg
       61 ttggtcagca caaaaagatg ggaaaaatct tccagtcaaa aaagcacaag gaggatttct
      121 atcagttact attcctaaag gaaagggacg tgttatcctt acctttattc ctaatggttt
      181 taaattaggg ttatctctat cttgcgtagg aattatcgct tatatgcttt tgtataagta
      241 catagatata aagtctaaat tactttaatc caaaaaaaat cagatttttt tatcaatttg
      301 gcaaatgagt caatatacta aaagcatctc aagagctaga caaatattct aatacttgag
      361 atgcttttat cgattttact aataaactca aataatataa aggtcagatt tttcgtgagg
      421 gttaatgtta aactactctc tttcaaagat tgaaatgaca ataatcatag ctgatacgat
      481 ttcaatcttt tgactcctta cagtaatctt atattcacca gtattaaatg agaggcatgc
      541 tttcaaaatg ttaaaaacta agcttcagtt aatgtttgta attaaactta atgttatcat
      601 ctatacaatt aaagaacaga acttctattt taatgcaaaa tacaatcctg ttagttcaca
      661 atgctgtatt tagtggttta ataattagta aattgagtat ttgacctgag tatctgtttt
      721 actaaataaa aaaatcctaa gattatctta ggaaatatct tatcactccg gcaataggac
      781 tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc tatgccggaa
      841 aaagaaaaaa attgccaaaa tgacaattgc gatagtccgt acgggattcg aacccgtgtt
      901 accgccgtga aaaggcggt
//
LOCUS       DATURV010000139          918 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DATURV010000139 DATURV010000000
VERSION     DATURV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.139"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..874)
                     /locus_tag="VCF75_001683"
     CDS             complement(<1..874)
                     /locus_tag="VCF75_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2547204.1"
                     /translation="MTIIILQIMRFGVYSSVDSSLVSVSNNASSYANRTMARISSFYF
                     DTENNIIKALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNYQNFHLDKRRLG
                     SIETTSLMNFYGQEEKYHTITVRVHIKNYPAVAYMMAVVNVEQLDRANERYERIIIIV
                     MSVFWLISILASIYLAKWSRKPILESYEKQKMFVENASHELRTPLAVLQNRLESLFRK
                     PNETILENSEHLASSLDEVRNMRILTTNLLNLARRDDGINPQWTHLDTDFFNAIFENY
                     ELVAKEYGKIFYFQN"
BASE COUNT          327 a          169 c          132 g          290 t
ORIGIN      
        1 ggttctgaaa ataaaatatt tttccatatt ctttagcaac tagttcataa ttctcaaaaa
       61 tagcattaaa aaaatctgta tctaaatgag tccactgtgg attaatgcca tcatctcgtc
      121 ttgctaaatt taataaatta gttgttaaga tgcgcatgtt gcgaacctcg tctaaactag
      181 aagcgagatg ctcactattt tctaatatcg tttcgttggg cttacgaaaa agcgattcca
      241 gacgattctg taagaccgcc aaaggggtcc ttagttcatg actagcattt tcaacaaaca
      301 ttttttgttt ttcatagctt tctaaaatag gttttctgct ccacttggct aaataaatac
      361 ttgctaaaat agaaattagc caaaaaacac tcataactat aataataatg cgctcataac
      421 gttcattagc gcggtctaat tgttccacat ttactactgc catcatatag gcaactgcag
      481 gataattttt gatatgaacc cttacagtta tcgtatggta tttttcttct tgtccataaa
      541 aattcattaa actggtggtt tcaatactcc ccaaccggcg tttatctaaa tgaaaatttt
      601 gatagttaga aaacgcatca aaagcattta aaattgttcc attagcacta aacaaaatga
      661 tatctgtatt agctgcaggc gttcctaata acttagaact atctgaatca ggcagcgcct
      721 taataatgtt attttcagta tcaaagtaaa aagaagatat tctagccatc gtacgattag
      781 catagctact tgcattatta ctaacagaaa ctaaactact atcaactgac gaataaacgc
      841 caaaccgcat aatctgtaag ataataatag tcataatgac aaaaatacct gtaaaaaccg
      901 caaaaaaatg aaaaaagt
//
LOCUS       DATURV010000140          913 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DATURV010000140 DATURV010000000
VERSION     DATURV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.140"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            30..842
                     /locus_tag="VCF75_001684"
     CDS             30..842
                     /locus_tag="VCF75_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2547205.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
BASE COUNT          303 a          190 c          161 g          259 t
ORIGIN      
        1 cttggagcaa aatttgctat aatttaatta tgactaaaaa attgattgcc attgatttgg
       61 atggaacctt attacatcat gacaacacca tctctaccta tactcaaaaa accatcaaag
      121 ctgtccaaga caagggccac cacgtcatta tttcaacagg ccgtccttac cgcatggcac
      181 tggactacta tctgcagctc aatttaaaga ccccaatcat tacttttaat ggggccttga
      241 cccacatgcc tgaacaaaaa tgggcctatg aacataatgt cactttggac aaaggctacc
      301 tactaaggtt actaaaatat caggatgatt tccagatgga ttttattgcc agtgagtatc
      361 gaaaaaatgt ctacatcacc atgaccaatc cagaaagtat cgacccgcaa ctctttggtg
      421 ttgatgaaat tactcaggac atggcattag agattaccaa aatcactcga aatccaaacg
      481 ccttattgat gcaaacgcat cacgaagaca agtatgcctt agctaaaaac atgcgtgctt
      541 tcttcaaaga tgaaattgaa attgattctt ggggaggccc tctcaacatt cttgaaattt
      601 cgtctaagaa tgtcaataaa gcttacgctc tgaactatct ccttggaatt tacaatatgg
      661 ataaaaagga tttaatcgcc tttggtgatg aacacaatga cactgagatg ctagcttttg
      721 ctggaactgg ttatgctatg aaaaatgcta gccctgtgct actgccttat gctgatcagc
      781 agcttaattt ttccaacgaa gaagacggtg ttgccaaaaa attggaagaa cttttcttgt
      841 agtagcaaac gaaaccacta tttttcagaa aatggtggtt tttcttctgt caacagctat
      901 caaaaaatga aaa
//
LOCUS       DATURV010000141          890 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DATURV010000141 DATURV010000000
VERSION     DATURV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.141"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..719)
                     /locus_tag="VCF75_001685"
     rRNA            complement(<1..719)
                     /locus_tag="VCF75_001685"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          267 c          167 g          272 t
ORIGIN      
        1 ctccatatat ctacgcattt caccgctaca catggaattc cactctcccc ttctgcactc
       61 aagttctcca gtttccaaag cgtacattgg ttgagccaat gcctttaact tcagacttaa
      121 aaaaccgcct gcgctcgctt tacgcccaat aaatccggac aacgctcggg acctacgtat
      181 taccgcggct gctggcacgt agttagccgt ccctttctgg ttagttaccg tcacttggtg
      241 gattttccac tcccaccatc attcttctct aacaacagag ctttacgatc cgaaaacctt
      301 cttcactcac gcggcgttgc tcggtcaggg ttgcccccat tgccgaagat tccctactgc
      361 tgcctcccgt aggagtctgg gccgtgtctc agtcccagtg tggccgatca ccctctcagg
      421 tcggctatgt atcgtcgcct tggtgagcct ttacctcacc aactagctaa tacaacgcag
      481 gtccatctca tagtggagca attgcccctt ttaaattact aacatgcgtt agtctctctt
      541 atgcggtatt agctatcgtt tccaatagtt atcccccgct atgaggtagg ttacctacgc
      601 gttactcacc cgttcgcaac tccttgaacc ggtgcaagca ccagttctca gcgttctact
      661 tgcatgtatt aggcacgccg ccagcgttcg tcctgagcca ggatcaaact ctctttaatt
      721 tgcgtttgaa cttactcgtt ccttctgtcg ctgacagatt tatttctcgt ttcttgacgg
      781 gtaatatgtc tccatatcac cctcacgttt ggttcgtctt attcagttct caaaggtctt
      841 aacgtctcct aagagacaac ttctttattc tatcaggttt ctcatagcct
//
LOCUS       DATURV010000142          876 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DATURV010000142 DATURV010000000
VERSION     DATURV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.142"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            180..875
                     /locus_tag="VCF75_001686"
     CDS             180..875
                     /locus_tag="VCF75_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
                     /protein_id="HEP2547206.1"
                     /translation="MEHQIERLIAEDIYIKVSFGVKQSGLIFIPNYQLDIRKEENHKK
                     YKVYIRETAQFFIYNKEASELNRYMRGHELICQLTNDSKSIPKRRRQTIDTLKKKIEE
                     ISLLIELDTENKPYQDIKDDIVKDMAQLDLTITELQDHIAHLNKVAEVLLNLNNNDIE
                     NRRLARYDYAKMNLTAAIKIEEVEKEIETSQNELNISIDEYEYLVRRLEKFGEILSDS
                     KIIDTSRNEIQWE"
BASE COUNT          336 a          125 c          182 g          233 t
ORIGIN      
        1 aagattactt taaaaaataa ggaagtcggt gtttcagatc gattagaaag tctacagaaa
       61 caatacatgc ttttcaagaa gaacgagata aagagaaggt atccactgaa gagagtaagg
      121 aagtcttcaa gaaatacaag gggcaacgag ataccatcca tgagtttgaa gttgaacttg
      181 tggaacatca aattgagagg ttaattgctg aggacattta tatcaaggtg tcttttggtg
      241 taaagcagag tggtcttatt ttcattccta actatcaatt ggatattagg aaagaagaaa
      301 atcataaaaa atataaagtc tatattcgtg agaccgcaca attcttcatt tacaacaagg
      361 aggcgtctga acttaatcgc tatatgcgag ggcatgagct catttgccag ttaaccaatg
      421 atagcaaaag tattccaaaa agaagacgac aaaccattga tacacttaag aaaaagattg
      481 aagaaataag tctcctcatt gagttagata cggagaataa gccttatcaa gatattaaag
      541 atgacattgt taaagatatg gctcaattag atttgaccat tacagagttg caggaccata
      601 ttgctcatct caataaggtt gcggaggtct tgcttaatct gaacaacaac gatatagaga
      661 accgccgttt ggccagatat gactatgcca agatgaactt gactgcagct ataaaaatag
      721 aagaagttga gaaagagatt gaaacttctc aaaatgaact taatatatcc atagatgagt
      781 atgaatatct agtaagaagg ttggaaaagt ttggagagat cttgagtgat agcaaaatta
      841 tcgatacttc tcgaaatgaa atacaatggg agtaat
//
LOCUS       DATURV010000143          849 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DATURV010000143 DATURV010000000
VERSION     DATURV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.143"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            48..320
                     /gene="rpsP"
                     /locus_tag="VCF75_001687"
     CDS             48..320
                     /gene="rpsP"
                     /locus_tag="VCF75_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEP2547207.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            330..569
                     /locus_tag="VCF75_001688"
     CDS             330..569
                     /locus_tag="VCF75_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEP2547208.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
BASE COUNT          277 a          141 c          151 g          280 t
ORIGIN      
        1 tagctgtatt tcttatacat tactaaaaga aaagagacta tttaaaaatg gcagtaaaaa
       61 tccgtttaac tcgtatgggt tctaagaaaa aacctttcta ccgtattaat gttgcagatt
      121 cacgtgcacc acgtgatggt cgttttatcg aaacagttgg aacatacaac ccattagtgg
      181 ctgaaaatca aattactatt aaagaggatc gtgttcttga gtggttatct aaaggagcgc
      241 agccttctga taccgttcgt aacatcttgt ctaaagctgg tgttatggct aagttccacg
      301 accaaaaatt ctctaaataa taacagccta tggataccat tgaaaatctt attatcgcta
      361 ttgtgaaacc tttgatttca caaccagata atcttaccat taaaattgaa gatactcctg
      421 actttttaga gtatcatctt gacttggatg ctcaagacat cggtcgtgtt atcggcaaaa
      481 aaggtcgaac tattactgcg ataagatcga ttgtctactc ggttccaaca ctaggaaaaa
      541 aagttcgctt agttattgat gagaaataat cactaacttg agatggattg aaaagcttgt
      601 tgacttaaag tcaacaagct ttttgaatag caaaaatctt aatatttctg aaatgaaaag
      661 attagatttt tctgtcttta actaaaaaga agttaaagaa tggcttagct gattgccttt
      721 ataagagatt atactggttt ttgatagctg ctgtcatgta aaatagctac aattcatctt
      781 acgagattga ccttagcatt gccagtgcag tctttatttt ccaacctgct gtttgttgta
      841 tgtttatag
//
LOCUS       DATURV010000144          798 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DATURV010000144 DATURV010000000
VERSION     DATURV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.144"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            31..717
                     /locus_tag="VCF75_001689"
     CDS             31..717
                     /locus_tag="VCF75_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2547209.1"
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFK
                     GYDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKP
                     FSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYR
                     EYEILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKT
                     IRGIGYKWDV"
BASE COUNT          280 a           91 c          158 g          269 t
ORIGIN      
        1 taagtatttc attatttaaa aggggtaata atgtataaga ttttagccat agatgatgat
       61 aaagaaattc tgaagcttat gaagactgct ttggagattg aaaattacca tgttataacc
      121 tgtcaagaga tagaattacc aatagttttt gatgatttta aaggatatga tttgatctta
      181 ttggatatca tgatgcctaa tataagtgga actgagtttt gttataaaat tcgggaagaa
      241 gttcattctc caattatttt tgttagtgct ttagatggcg ataatgaaat tgtccaagct
      301 ttaaatatag ggggagacga ttttattgtg aaaccattta gcttaaaaca attcgtagct
      361 aaagttaact ctcatttgaa gagagaagag agagcaaaga taaagaatga ggctgaggag
      421 agagtgaagc gaagttttcc acctatagaa atctatctag aggaacgtat gttatatatt
      481 gataaacagc cgttattcct gacttataga gagtacgaaa ttttagaatt actgtcacgt
      541 catccgtata aagttttcac aaaagaagag atatatgaac aagtatatag cgatgaagct
      601 tcagcattgt ttcattctat ttcagaatat atctatcaga ttagaatgaa attttcaagt
      661 tttggaatta atccaataaa aactattcgc gggattggtt ataaatggga tgtttaaaaa
      721 atattcaata aaaaatcgtg tttggcgcgc cgtcgttgaa attgtctttg gagtttgtat
      781 cactgtttta atgattgc
//
LOCUS       DATURV010000145          775 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DATURV010000145 DATURV010000000
VERSION     DATURV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.145"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            62..>775
                     /gene="mf2"
                     /locus_tag="VCF75_001690"
     CDS             62..>775
                     /gene="mf2"
                     /locus_tag="VCF75_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEP2547210.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAARVRTYPNVSHANTH
                     YKNTVSSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVAMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPN"
BASE COUNT          259 a          139 c          144 g          233 t
ORIGIN      
        1 tagaaaagtg caatatttga tccactctaa aagctaaaaa tggaaaggtt taggtgttat
       61 catgaaatta tctaaacaaa aggcaagttt gcttaccgct gttttactgt tactttctct
      121 ttctataacg acaataactg ttgacgcagc tagggtacga acttatccga atgtatccca
      181 tgcaaacaca cattataaaa atactgtttc tagtaagctc ttacctttta ctgctaatta
      241 tcagttacaa cttggtgagt tggataacct taaccgcgct accttttcac atatccaact
      301 gcaagataga cacgaaacta aagatgtacg cactaaaata aattatgacc ctgtgggatg
      361 gcacaactat caatttccat acggagatgg ctcaaaatct tcatgggtaa tgaatcgtgg
      421 gcatctagtc ggatatcaat tttgtggatt aaacgatgaa ccaagaaatt tagtggcaat
      481 gacagcctgg ctaaacacag gtgcttattc aggagcaaat gacagcaatc ctgaagggat
      541 gttatactat gaaaaccgtt tagattcttg gcttgcactt caccctgact tttggctaga
      601 ttataaagtc actcctatat atagtggcaa tgaagtagtg cctcgacaaa ttgaattaca
      661 gtatgttgga attgattcat ctggtgagtt gcttactata aggctaaata gtaataaaga
      721 aagtattgat gaaaatggtg ttactacagt aatcttagaa aactctgctc caaat
//
LOCUS       DATURV010000146          658 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DATURV010000146 DATURV010000000
VERSION     DATURV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.146"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(188..601)
                     /locus_tag="VCF75_001691"
     CDS             complement(188..601)
                     /locus_tag="VCF75_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP2547211.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRPDKHDYK
                     LDLSCGRIELEIFGKVTSDPYYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
BASE COUNT          232 a          128 c           91 g          207 t
ORIGIN      
        1 tattttgctt ttcctatagt tattctatta aaaaacttcg acaagattac tccaaaaatt
       61 gctcttcact ttgaaagtta accaaatcat ttaacgatca aaagagatta atcaagcaaa
      121 agaagataca acaaataact atttttaacg agtttaatag cacgatccaa tcgttaacct
      181 aataagtcta ttcatgtaat tctaaaggca aaccatccgg atcaaaaaag aaagtcatct
      241 tctcacctgt gtaatcatca tgcctaatag gctccactgg aattcctaag ctctttagat
      301 catctacata actctcaatg ttggtaacac gaaaagcaag gtgcctcaaa ccacaggcct
      361 cacttttaaa ttctggttga cttacacgtt taggaggagc ttgatagtag gggtcacttg
      421 tcaccttacc aaaaatctcc aattcaatac gaccacaaga taaatctaat ttgtaatcat
      481 gcttgtcagg acgataattt tctctgataa tttcaaatcc gagtttattc acataaaaat
      541 cttttgacag atgataatca gatacaataa tagcaacgtg gtgaatggca tttagtttca
      601 tcatatttct cctttcaaaa aaagtaggat gagataaatt ttctctatgg tcctactt
//
LOCUS       DATURV010000147          656 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DATURV010000147 DATURV010000000
VERSION     DATURV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.147"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            9..635
                     /locus_tag="VCF75_001692"
     CDS             9..635
                     /locus_tag="VCF75_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP2547212.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRKDKGYYVLRSIKQA"
BASE COUNT          244 a           87 c          129 g          196 t
ORIGIN      
        1 atgatagaat gaaaaagtac cttaaaaaga taggaaaaat gatgacaaag atgtattatg
       61 atgaaaatcc agatagccta cacgatattc atgaagtaaa agtagagctg cttaatcatc
      121 cctttacttt tttaacggat tcaggtgtct tttcaaagaa aatggttgat ttcggtagtc
      181 aagtattatt agagacacta aatttcaagg agaacgaacg tgtactggac ttaggttgcg
      241 gctatggtcc tcttgggatt agtctagcca aggttcaaag ggtagacgca accttagtgg
      301 acatcaataa tcgtgctctt gatttagcta gaaaaaatgc aactaataac caagtggcag
      361 taactatttt tcaatcaaat atttatgaaa atattagtgg ccattttgag catattatta
      421 gtaatcctcc tattagagca ggaaagagag tagttcatag cattattgaa aaaagcatag
      481 attttttagt ggttaatggt gacttaacga ttgtgattca aaaaaaacaa ggagctccta
      541 gcgctaaagc aaaaatggca actatttttg gtaatgttga gatactaagg aaagataaag
      601 gttattatgt attaagaagt ataaagcaag cctaatttaa ataatcaaca attgct
//
LOCUS       DATURV010000148          619 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DATURV010000148 DATURV010000000
VERSION     DATURV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.148"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            94..>619
                     /gene="rgg4"
                     /locus_tag="VCF75_001693"
     CDS             94..>619
                     /gene="rgg4"
                     /locus_tag="VCF75_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEP2547213.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLELYLAYLDIEG"
BASE COUNT          228 a           71 c          122 g          198 t
ORIGIN      
        1 aaaaaacgaa tattttcttt aaaaatatta aaaaggttag tttttaatta gacgctgtga
       61 tataataaag aaaacagttg ttggagagaa accatgttag aacattttgg tggaaaagta
      121 aaagtgttaa gacttgaaaa gaggattagt cgcgaggact tgtgtgggga tgagtctgaa
      181 ctttctgttc gtcaattagc acggatagaa ctaggtcaat ccataccaag tttaagtaag
      241 gttattttta ttgcaaaagc cttaaacgtt agtgtcggtt acttaactga tggtgctgat
      301 ttagaactac ctaagcgtta caaagaatta aaatacctta tcttaaggac accaacttac
      361 atggatgatg gaaaattaca agtacgagaa gagcagtttg atgaaatttt tgaggattat
      421 tatgataaat taccagagga agagaaaata atcattgatt gtttacaggc aactttagat
      481 actttattga gtgagaatac taactttggc attgacttac ttcaagaata ttttaatcaa
      541 ataaagacta aggtacgctt taggcaaaat gatttaatac ttctagaatt atatttagct
      601 tatcttgata ttgagggaa
//
LOCUS       DATURV010000149          610 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DATURV010000149 DATURV010000000
VERSION     DATURV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.149"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..>610)
                     /locus_tag="VCF75_001694"
     CDS             complement(<1..>610)
                     /locus_tag="VCF75_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEP2547214.1"
                     /translation="LVTNDFLISDLRPPICNINSLDYLLSKLEEGTLCTDLTTLKKMI
                     EDYNDLNIGEGRDLLLQIFDKMESIYKYDNGGNYLKVDSLAEYEGDFNILSETARESI
                     ERLAEMFVKLNTNVKRRGGRKNSLEEYQLKFIGKYGENCSIPFVEVINKDAGIGFPEY
                     YKGGEGEAIELSDPIMQMFEKKYEEALLNGGHIEFYSKDLDD"
BASE COUNT          186 a          123 c           64 g          237 t
ORIGIN      
        1 aaatcatcta aatcttttga ataaaattcg atatgccctc catttaatag agcttcctca
       61 tatttctttt caaacatttg cattattgga tcgcttaact ctattgcttc accttctcct
      121 cctttgtaat attcaggaaa acctatgcct gcatctttat ttattacttc aacaaaggga
      181 attgagcagt tttctccata tttgccaata aatttcaact gatactcttc aagtgaattt
      241 ttcctccccc ctcttctctt tacatttgta tttaatttta caaacatctc tgcgagtcgt
      301 tctatacttt cccgagccgt ttcagataga atattaaaat cgccttcata ctctgctaat
      361 gaatcaactt ttaagtaatt tccaccgtta tcatatttat aaatactttc cattttatca
      421 aaaatctgca aaagtaagtc tctaccttcc ccaatattta aatcgttgta atcttcaatc
      481 atcttcttta aagtagttaa gtcagtacat agtgttcctt cttctaattt agatagtaga
      541 taatctaagg aattgatatt acaaattggt ggtcttaaat ctgaaattaa aaaatcattt
      601 gtaactaagt
//
LOCUS       DATURV010000150          591 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DATURV010000150 DATURV010000000
VERSION     DATURV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.150"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..>591)
                     /locus_tag="VCF75_001695"
     CDS             complement(<1..>591)
                     /locus_tag="VCF75_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEP2547215.1"
                     /translation="FLIIYIYTQPKYAIELMTTLGEVALGSQTIFNILFMPAFVMNLL
                     ILFFRPYITQMAIALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSMLYG
                     TNLTDYWVDFMLIMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFV
                     MKYHILGAALSFLITMLVWLGLSIMIYLFIMNRFKK"
BASE COUNT          246 a          104 c           75 g          166 t
ORIGIN      
        1 ccttcttaaa tctattcata ataaatagat aaatcataat ggataatccc aaccaaacca
       61 acattgttat taaaaagctc aaagcagctc ctaaaatgtg atatttcata acaaaaagat
      121 tggtaattaa taacgaaatc aaaaatcctc ctgtatatgg aataagaaga agttgctgtt
      181 ttctcatagc ggttaagata ttatcgataa ctgtcgcaaa actaccaatc gagcctccta
      241 gcataatcaa cataaagtct acccaataat ccgtaaggtt agtaccatat agcattgata
      301 aaaagggaat accaaacaaa ccacttccaa ctaacgctat cagtgaaaaa actcctaaat
      361 aagcaaacaa ttgaacttgt atcttattaa actcttttat ttgacctctg attaaagcaa
      421 tagccatctg tgtaatataa ggtctaaaaa ataaaattag caaattcata acaaaggctg
      481 gcataaataa aatattaaaa attgtttgag agcccaaagc aacttcacct aaagtcgtca
      541 ttagttcaat agcgtatttt ggttgagtat aaatatagat aatcaaaaaa c
//
LOCUS       DATURV010000151          562 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DATURV010000151 DATURV010000000
VERSION     DATURV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.151"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            63..>562
                     /locus_tag="VCF75_001696"
     CDS             63..>562
                     /locus_tag="VCF75_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2547216.1"
                     /translation="MKKTKLIFSLTSIFIAIISRPVFGLEVDNNSLLRNIYSTIMYEY
                     SDTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKAGDKIAVFSVPFDWNYL
                     SEGKVIAYTYGGITPYQKTSIPKNIPVNLWINGKQISVPYNEISTNKTTVTAQEIDLK
                     VRKFLIA"
BASE COUNT          212 a           85 c           84 g          181 t
ORIGIN      
        1 tttcttttga aagtgttata ttaaaacata ataaaataat aactcctgaa aagaggctat
       61 ttatgaaaaa aacaaaactt attttttctt tgacttcaat attcattgca ataatttctc
      121 gtcctgtgtt tggattagaa gtagataata attcccttct aaggaatatc tatagtacga
      181 ttatgtatga atattcagat acagtaattg attttaaaac cagtcataac ttagtgacta
      241 agaaacttga tgttagagat gctagagatt tttttattaa ctccgaaatg gacgaatatg
      301 cagccaatga ttttaaagct ggagataaaa tagctgtgtt ctccgtccca tttgattgga
      361 actacttgtc agaaggaaaa gtcatagcat atacgtacgg cggaataaca ccctaccaaa
      421 aaacttcaat acctaaaaat atccctgtta atttatggat taatggaaag cagatctctg
      481 ttccttacaa cgaaatatca actaacaaaa caacagttac agctcaagaa attgatctaa
      541 aggttagaaa atttttaata gc
//
LOCUS       DATURV010000152          545 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DATURV010000152 DATURV010000000
VERSION     DATURV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.152"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..471
                     /locus_tag="VCF75_001697"
     CDS             <1..471
                     /locus_tag="VCF75_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEP2547217.1"
                     /translation="YTSNILDLDLNYLPTRMAWCYGTPGTAVALFKMCQLIDLNFIDD
                     INESLIAQMRSSDKNVYSLTMCHGLAGVTFIYDLIGKNYSSERLTRFAEELRQQIITS
                     FSTTKVFGYTDREKFGEIEVELESIGILQGVSGIVLFLLNPYSDEELLWQRIFI"
BASE COUNT          185 a           69 c          110 g          181 t
ORIGIN      
        1 tatactagta atattttgga tcttgattta aattatcttc ctactagaat ggcatggtgt
       61 tacggtacgc ctggaactgc agttgccctt tttaaaatgt gtcaattgat agatttaaat
      121 tttattgacg atattaatga aagtttaatt gctcagatga gaagttcaga caaaaatgtc
      181 tattctctta caatgtgcca tggtttggca ggagtaactt ttatatatga cttgattggt
      241 aaaaattatt caagtgagag gctaactcgc tttgcagaag agcttcgcca acaaataatt
      301 acatcgtttt caactacaaa agtatttggg tatactgaca gagaaaaatt tggagaaata
      361 gaagttgaac tggagtcaat agggatattg caaggggtat cagggatagt gttattctta
      421 ttaaatcctt acagtgacga ggagctattg tggcaaagaa tatttattta atttgaaaag
      481 gagaacaaaa tgtataaggt atttccaact ggtatgattc gtattccatt gcaaaaacaa
      541 gaatt
//
LOCUS       DATURV010000153          524 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DATURV010000153 DATURV010000000
VERSION     DATURV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.153"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..>524)
                     /locus_tag="VCF75_001698"
     CDS             complement(<1..>524)
                     /locus_tag="VCF75_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEP2547218.1"
                     /translation="ANIGSGQAGRSFGRFYGLSETVRETIKNLNHQNNTNVELSFVPK
                     QIRLANVIQNYSDESYNTSFFTTSWDSENELRLEDIYIRYSDGKFHFTTIDGKNELKF
                     TMNNMLNSDSQSRVLRLLVDLSEFEYGLSHWSLFPWDILAQERVYIPEILFEDITIAT
                     AQWNLSVIREEIIH"
BASE COUNT          173 a          107 c           65 g          179 t
ORIGIN      
        1 tgatgtataa tctcttctct aattactgat aaattccact gagcagtggc aatcgtaatg
       61 tcttcaaata aaatttcagg aatatatact ctttcctgag ccaaaatatc ccaaggaaat
      121 aatgaccaat gcgataaacc gtactcaaac tcagataaat caaccaacaa tctcaaaacc
      181 ctgctttggc tatcggaatt tagcatatta ttcatggtaa atttcaactc atttttccca
      241 tcgatagttg taaaatgaaa cttaccatca ctatatctta tataaatatc ctccaatcgc
      301 agttcgttct ctgaatccca tgatgtggta aaaaatgaag tattataaga ctcatcacta
      361 taattttgaa ttacattcgc aagtcttatc tgtttaggaa caaatgacaa ctctacattc
      421 gtattatttt gatggtttaa attcttaatt gtttctctta ctgtttcgct taatccatag
      481 aatcttccaa agcttctacc tgcttgtcca gaaccgatat tagc
//
LOCUS       DATURV010000154          512 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DATURV010000154 DATURV010000000
VERSION     DATURV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.154"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(49..231)
                     /locus_tag="VCF75_001699"
     CDS             complement(49..231)
                     /locus_tag="VCF75_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEP2547219.1"
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITG
                     GAAAFVCVASGIAAYQ"
     gene            complement(246..478)
                     /locus_tag="VCF75_001700"
                     /pseudo
     CDS             complement(246..478)
                     /locus_tag="VCF75_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
BASE COUNT          177 a          132 c           51 g          152 t
ORIGIN      
        1 tgggaaagta ttagacaaat taaaccgagt attataacta taatcatatc attgataagc
       61 tgcaattcca gatgctacac atacaaatgc tgccgcacct ccagttattg gacctcctgc
      121 tgcagctata agcatcccac ctaagcaccc tccgatagca gatccaatat ttccttttcc
      181 accagaaata tttactaatt cttcaaactg taattctaaa aaattatcca tttttcacct
      241 caaaattatt ttccattaaa taacatacca ccaacgcaag ttaatccacc cgcaatagca
      301 cctacatgtg cacctgcaaa cgcacctgca actcctccaa cacctaaagt agccaaccac
      361 ctgcaatagc acctgaagta ataccaccaa ttaaatgatt tttacaatct gtagcactat
      421 aacctccaat aatatggaac aactcttctt tttgtatttc ttctgcaaat tttatcataa
      481 ctttaaaaac ctttctaaaa tgttatattc at
//
LOCUS       DATURV010000155          425 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DATURV010000155 DATURV010000000
VERSION     DATURV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.155"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            <1..>425
                     /locus_tag="VCF75_001701"
     CDS             <1..>425
                     /locus_tag="VCF75_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEP2547220.1"
                     /translation="KKYIEQKYKNISFSIDYHYYLTTTGSCIDFGEEGAVGRGNKTHG
                     IISSFRPNTMEAPAGKNCTYFVGKVWGFLSDTIAKEIYEAFNTPCQIIMQLNIGSKLY
                     RPTHLFIQTEESVDQERVLEIVNRHLNNGKQNTNLILST"
BASE COUNT          161 a           54 c           71 g          139 t
ORIGIN      
        1 aagaaatata ttgaacaaaa gtataaaaac ataagctttt ctattgatta ccattattat
       61 ttaactacta caggttcttg tattgatttt ggagaagaag gtgcagttgg tcgaggtaat
      121 aaaactcatg gtatcatatc aagttttagg ccaaatacta tggaagcacc agctggaaaa
      181 aattgtactt attttgtagg aaaggtttgg ggatttttgt ctgatactat tgccaaagaa
      241 atttatgaag cattcaatac tccctgtcaa atcattatgc aattaaatat tggatcaaaa
      301 ttgtatagac caacccattt atttattcag acagaagaaa gtgttgatca agaaagagta
      361 ttagaaattg ttaacaggca tttaaataat ggtaaacaaa atactaattt aatcttgtca
      421 actca
//
LOCUS       DATURV010000156          417 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DATURV010000156 DATURV010000000
VERSION     DATURV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.156"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..417)
                     /locus_tag="VCF75_001702"
     CDS             complement(<1..417)
                     /locus_tag="VCF75_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEP2547221.1"
                     /translation="MVVTRLLTSSDSDIYAFAYSFANMMVVVGLFQVRNYQATDINEK
                     YSFSQYLVARLMTCLLMLAITVIYLTLTKTDSYKSTIVFLVCFYRSTDAFSDLYQGMF
                     QQHERLDIAGKSLAYRNTLIFMVYTAIILYSKNLTLA"
BASE COUNT          153 a           71 c           65 g          128 t
ORIGIN      
        1 tgctaaagta agatttttgg aatataatat tattgctgtg tacaccataa aaataagagt
       61 atttctgtag gctagcgatt tacctgcaat atccagccgt tcatgttgtt ggaacattcc
      121 ctgatataaa tccgaaaaag catctgtcga tctatagaaa cagactagaa agacaattgt
      181 actcttgtag ctatctgtct tagttaacgt taaataaatt actgttatag ctaacattaa
      241 taaacaagtc atcagtcttg ctactaagta ctgactaaag gaatattttt cattaatatc
      301 tgttgcttga taattacgaa cctgaaaaag tccaacaact accatcatat tagcaaaaga
      361 ataggcaaag gcgtaaatat ctgaatcaga cgaagttagt aacctagtaa ccaccat
//
LOCUS       DATURV010000157          399 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DATURV010000157 DATURV010000000
VERSION     DATURV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.157"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
     gene            complement(<1..>399)
                     /locus_tag="VCF75_001703"
     CDS             complement(<1..>399)
                     /locus_tag="VCF75_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2547222.1"
                     /translation="DKLDKGLLIIIFVSITSLFVNRILFNFLFLMVFTILHLYPLLVI
                     VLYYRSFRQQISVVRRSLILFSLLLLVILGSELYGEMLDVNQAFNNLGWYLFPLIMSV
                     IYYFKTIHDKLSFVTQRWLGDYKARIELLFL"
BASE COUNT          170 a           65 c           49 g          115 t
ORIGIN      
        1 agaaataata gttctatacg agctttgtaa tcacctaacc atctttgggt aacaaaactc
       61 aatttatcat gtatggtctt aaaatagtat attacactca ttataagggg aaacagatac
      121 catcccaaat tattaaaggc ttgattaaca tctagcattt ctccataaag ttcactacca
      181 agtattacca gcaaaagtaa agaaaatagt atcaatgaac gtctaacaac tgatatttgt
      241 tgcctaaagg acctataata tagcactata actagtaacg gatacaggtg taaaatagta
      301 aaaaccatca ggaataaaaa attaaataaa attctattaa caaaaaggct agttatagaa
      361 acaaaaataa ttattaacaa gcccttatct aacttatct
//
LOCUS       DATURV010000158          380 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes isolate SH24664A
            SAMEA114250740-rid20193903.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DATURV010000158 DATURV010000000
VERSION     DATURV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114250740
            Sequence Read Archive: ERR11829052
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001982666.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001982666.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 22:06:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,703
            CDSs (total)                      :: 1,663
            Genes (coding)                    :: 1,618
            CDSs (with protein)               :: 1,618
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 28 of 45
            Pseudo Genes (incomplete)         :: 16 of 45
            Pseudo Genes (internal stop)      :: 14 of 45
            Pseudo Genes (multiple problems)  :: 12 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114250740-rid20193903.denovo.158"
                     /isolate="SH24664A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Portugal"
                     /collection_date="2023"
BASE COUNT          140 a           54 c           63 g          123 t
ORIGIN      
        1 ttaagcaaat gatcttagta ctaaggttaa aagattatcg tgaaataaag ttttgacaca
       61 agtgtaatta atagaggaac ttgaagtatc cggtaaaact atcttgaagt gaaaaaacga
      121 gtagacctcc cctgataaat acaatatact agccatttca gagcaattaa gagtttccag
      181 tgaagtgctt ttgatagaga gctaagaaaa aaacagtcaa ggtttaagaa ctgcaaatta
      241 gcatttaaat tattttttgt tgatgacagt tgtctttatt accataacta taacttctta
      301 taatatagtc ttcttattca tcagtggtgg ctcgtctcat atctgacctt aaaatattaa
      361 actgatcatc agatgataga
//
