LOCUS       DATUYR010000001        76548 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.001, whole genome shotgun sequence.
ACCESSION   DATUYR010000001 DATUYR010000000
VERSION     DATUYR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 76548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76548
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.001"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            125..1339
                     /gene="sstT"
                     /locus_tag="VCI33_000001"
     CDS             125..1339
                     /gene="sstT"
                     /locus_tag="VCI33_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEP2830962.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGKQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVSVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(1394..2068)
                     /locus_tag="VCI33_000002"
     CDS             complement(1394..2068)
                     /locus_tag="VCI33_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEP2830963.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(2078..3469)
                     /locus_tag="VCI33_000003"
     CDS             complement(2078..3469)
                     /locus_tag="VCI33_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEP2830964.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSIEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(3539..4252)
                     /gene="rsmG"
                     /locus_tag="VCI33_000004"
     CDS             complement(3539..4252)
                     /gene="rsmG"
                     /locus_tag="VCI33_000004"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEP2830965.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            4402..4959
                     /locus_tag="VCI33_000005"
     CDS             4402..4959
                     /locus_tag="VCI33_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEP2830966.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            5006..5902
                     /gene="htpX"
                     /locus_tag="VCI33_000006"
     CDS             5006..5902
                     /gene="htpX"
                     /locus_tag="VCI33_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEP2830967.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMINALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            6136..6669
                     /locus_tag="VCI33_000007"
     CDS             6136..6669
                     /locus_tag="VCI33_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEP2830968.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            6936..7622
                     /gene="covR"
                     /locus_tag="VCI33_000008"
                     /gene_synonym="csrR"
     CDS             6936..7622
                     /gene="covR"
                     /locus_tag="VCI33_000008"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEP2830969.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            7628..9130
                     /gene="covS"
                     /locus_tag="VCI33_000009"
                     /gene_synonym="csrS"
     CDS             7628..9130
                     /gene="covS"
                     /locus_tag="VCI33_000009"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEP2830970.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHGVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            9345..9839
                     /gene="nrdR"
                     /locus_tag="VCI33_000010"
     CDS             9345..9839
                     /gene="nrdR"
                     /locus_tag="VCI33_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEP2830971.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            9823..10998
                     /locus_tag="VCI33_000011"
     CDS             9823..10998
                     /locus_tag="VCI33_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEP2830972.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGDLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            10999..11901
                     /gene="dnaI"
                     /locus_tag="VCI33_000012"
     CDS             10999..11901
                     /gene="dnaI"
                     /locus_tag="VCI33_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEP2830973.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLPDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            11964..13274
                     /gene="der"
                     /locus_tag="VCI33_000013"
     CDS             11964..13274
                     /gene="der"
                     /locus_tag="VCI33_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEP2830974.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDEIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            13481..16579
                     /locus_tag="VCI33_000014"
     CDS             13481..16579
                     /locus_tag="VCI33_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2830975.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASNTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMKDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNENRASMSIEEIKEILGLN
                     TH"
     gene            16822..17424
                     /locus_tag="VCI33_000015"
     CDS             16822..17424
                     /locus_tag="VCI33_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2830976.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            17464..18792
                     /locus_tag="VCI33_000016"
     CDS             17464..18792
                     /locus_tag="VCI33_000016"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEP2830977.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDTALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            18838..19320
                     /locus_tag="VCI33_000017"
     CDS             18838..19320
                     /locus_tag="VCI33_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2830978.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            19432..21006
                     /gene="mltG"
                     /locus_tag="VCI33_000018"
     CDS             19432..21006
                     /gene="mltG"
                     /locus_tag="VCI33_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEP2830979.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            21079..21561
                     /gene="greA"
                     /locus_tag="VCI33_000019"
     CDS             21079..21561
                     /gene="greA"
                     /locus_tag="VCI33_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEP2830980.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(22181..23104)
                     /gene="yidC"
                     /locus_tag="VCI33_000020"
     CDS             complement(22181..23104)
                     /gene="yidC"
                     /locus_tag="VCI33_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP2830981.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMAFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(23186..23464)
                     /locus_tag="VCI33_000021"
     CDS             complement(23186..23464)
                     /locus_tag="VCI33_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEP2830982.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGK"
     gene            23725..24462
                     /locus_tag="VCI33_000022"
     CDS             23725..24462
                     /locus_tag="VCI33_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEP2830983.1"
                     /translation="MVITSKTNQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            24501..25001
                     /locus_tag="VCI33_000023"
     CDS             24501..25001
                     /locus_tag="VCI33_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP2830984.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            25016..25705
                     /locus_tag="VCI33_000024"
     CDS             25016..25705
                     /locus_tag="VCI33_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEP2830985.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            25883..26125
                     /locus_tag="VCI33_000025"
     CDS             25883..26125
                     /locus_tag="VCI33_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEP2830986.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            26303..27097
                     /gene="racE"
                     /locus_tag="VCI33_000026"
     CDS             26303..27097
                     /gene="racE"
                     /locus_tag="VCI33_000026"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEP2830987.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSVQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLSPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVKHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            27094..28080
                     /locus_tag="VCI33_000027"
     CDS             27094..28080
                     /locus_tag="VCI33_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEP2830988.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQRKIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDIILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            28059..28580
                     /locus_tag="VCI33_000028"
     CDS             28059..28580
                     /locus_tag="VCI33_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP2830989.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            28577..29038
                     /locus_tag="VCI33_000029"
     CDS             28577..29038
                     /locus_tag="VCI33_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP2830990.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            29035..29781
                     /gene="xerD"
                     /locus_tag="VCI33_000030"
     CDS             29035..29781
                     /gene="xerD"
                     /locus_tag="VCI33_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEP2830991.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLIGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEAYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            29781..30485
                     /locus_tag="VCI33_000031"
     CDS             29781..30485
                     /locus_tag="VCI33_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEP2830992.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKTNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            30482..31033
                     /locus_tag="VCI33_000032"
     CDS             30482..31033
                     /locus_tag="VCI33_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEP2830993.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            31155..31877
                     /locus_tag="VCI33_000033"
     CDS             31155..31877
                     /locus_tag="VCI33_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP2830994.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            31874..32137
                     /gene="yidD"
                     /locus_tag="VCI33_000034"
     CDS             31874..32137
                     /gene="yidD"
                     /locus_tag="VCI33_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEP2830995.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            32315..32863
                     /locus_tag="VCI33_000035"
     CDS             32315..32863
                     /locus_tag="VCI33_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEP2830996.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      32972..33096
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33175..33738
                     /locus_tag="VCI33_000036"
     CDS             33175..33738
                     /locus_tag="VCI33_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2830997.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            33740..34393
                     /locus_tag="VCI33_000037"
     CDS             33740..34393
                     /locus_tag="VCI33_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP2830998.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILLIGLSRIYLGVHYPSDILAGFVLGFGILHFIYPFYKQKRFEWRFLLKQD"
     gene            34686..35606
                     /locus_tag="VCI33_000038"
     CDS             34686..35606
                     /locus_tag="VCI33_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEP2830999.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            35645..36199
                     /locus_tag="VCI33_000039"
     CDS             35645..36199
                     /locus_tag="VCI33_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP2831000.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            36332..37666
                     /locus_tag="VCI33_000040"
     CDS             36332..37666
                     /locus_tag="VCI33_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEP2831001.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            37744..38535
                     /gene="pflA"
                     /locus_tag="VCI33_000041"
     CDS             37744..38535
                     /gene="pflA"
                     /locus_tag="VCI33_000041"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEP2831002.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            38666..39601
                     /locus_tag="VCI33_000042"
     CDS             38666..39601
                     /locus_tag="VCI33_000042"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEP2831003.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            39677..40330
                     /locus_tag="VCI33_000043"
     CDS             39677..40330
                     /locus_tag="VCI33_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEP2831004.1"
                     /translation="MITVFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRDDLTLANYFYRLKRGEKPSAEQLDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(40375..41412)
                     /locus_tag="VCI33_000044"
     CDS             complement(40375..41412)
                     /locus_tag="VCI33_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP2831005.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLGLYTSLILLLVSLMGLALSLGESH
                     LSFLDLIHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGTNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(41373..42425)
                     /locus_tag="VCI33_000045"
     CDS             complement(41373..42425)
                     /locus_tag="VCI33_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP2831006.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLANAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(42415..43347)
                     /locus_tag="VCI33_000046"
     CDS             complement(42415..43347)
                     /locus_tag="VCI33_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP2831007.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(43373..44155)
                     /locus_tag="VCI33_000047"
     CDS             complement(43373..44155)
                     /locus_tag="VCI33_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831008.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(44401..45846)
                     /locus_tag="VCI33_000048"
     CDS             complement(44401..45846)
                     /locus_tag="VCI33_000048"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEP2831009.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMV
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIARHYL"
     gene            45934..47568
                     /locus_tag="VCI33_000049"
     CDS             45934..47568
                     /locus_tag="VCI33_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEP2831010.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSAIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFLIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLAAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLMALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYVFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            47736..48365
                     /gene="upp"
                     /locus_tag="VCI33_000050"
     CDS             47736..48365
                     /gene="upp"
                     /locus_tag="VCI33_000050"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEP2831011.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            48589..49179
                     /locus_tag="VCI33_000051"
     CDS             48589..49179
                     /locus_tag="VCI33_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002274529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEP2831012.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLTYGFIDEIMENNELK"
     gene            49674..49949
                     /locus_tag="VCI33_000052"
     CDS             49674..49949
                     /locus_tag="VCI33_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEP2831013.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIVYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKG"
     gene            50198..50833
                     /locus_tag="VCI33_000053"
     CDS             50198..50833
                     /locus_tag="VCI33_000053"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEP2831014.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            50851..51726
                     /locus_tag="VCI33_000054"
     CDS             50851..51726
                     /locus_tag="VCI33_000054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEP2831015.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHVNAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            52229..52552
                     /gene="yabA"
                     /locus_tag="VCI33_000055"
     CDS             52229..52552
                     /gene="yabA"
                     /locus_tag="VCI33_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEP2831016.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            52557..53420
                     /gene="rsmI"
                     /locus_tag="VCI33_000056"
     CDS             52557..53420
                     /gene="rsmI"
                     /locus_tag="VCI33_000056"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEP2831017.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            53447..53839
                     /locus_tag="VCI33_000057"
     CDS             53447..53839
                     /locus_tag="VCI33_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831018.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(53886..54515)
                     /locus_tag="VCI33_000058"
     CDS             complement(53886..54515)
                     /locus_tag="VCI33_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEP2831019.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTSENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDRIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQSLSQLTGTAIVHGTKII"
     gene            54814..55170
                     /locus_tag="VCI33_000059"
     CDS             54814..55170
                     /locus_tag="VCI33_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEP2831020.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGN
                     VLQVGYRKPYQELDLG"
     gene            complement(55244..56071)
                     /gene="xth"
                     /locus_tag="VCI33_000060"
     CDS             complement(55244..56071)
                     /gene="xth"
                     /locus_tag="VCI33_000060"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEP2831021.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRIVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            56305..57486
                     /gene="lctO"
                     /locus_tag="VCI33_000061"
     CDS             56305..57486
                     /gene="lctO"
                     /locus_tag="VCI33_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEP2831022.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYCSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDHKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            57752..62689
                     /gene="scpC"
                     /locus_tag="VCI33_000062"
     CDS             57752..62689
                     /gene="scpC"
                     /locus_tag="VCI33_000062"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEP2831023.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMTTTVAADELTTTSEPT
                     ITNHAQQQAQHLTNTELSSAESQSPDTSQITPKINREKEQPQGLVSEPTTTELADTDA
                     APMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKYES
                     HGMHVTGIVAGNSKEAAATGERFLGIAPETQVMFMRVFANDVMGSAESLFIKAIEDAV
                     ALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDPLA
                     TNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVDFK
                     DIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHGAL
                     GVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVSKA
                     PSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIAGA
                     SLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNIDG
                     AVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQKG
                     RFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRFRD
                     SQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGKHF
                     TGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNNQD
                     FAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTLLG
                     TAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDPET
                     NRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQADG
                     SFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKETL
                     KDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKGLK
                     NNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQYV
                     VTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVREEV
                     FYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVEDRA
                     GNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYYNN
                     SGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQIT
                     AHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRIEG
                     NTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSATAK
                     ALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            63462..64169
                     /locus_tag="VCI33_000063"
     CDS             63462..64169
                     /locus_tag="VCI33_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEP2831024.1"
                     /translation="MSKKKRKHYQVYDGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSVIL
                     TGIAFGMALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            64409..66409
                     /gene="metG"
                     /locus_tag="VCI33_000064"
     CDS             64409..66409
                     /gene="metG"
                     /locus_tag="VCI33_000064"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEP2831025.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEEVFRDEDGKVIGGIAPSGH
                     QVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADFSQLIDAQLADYHKHMEAVDYPRALEAIWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKCYPNEQELVGKKLQIVA
                     NLKPRKMMKQYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            66900..67913
                     /gene="nrdF"
                     /locus_tag="VCI33_000065"
     CDS             66900..67913
                     /gene="nrdF"
                     /locus_tag="VCI33_000065"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP2831026.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRSRVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYDSPFTDEETRISPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            67917..68405
                     /gene="nrdI"
                     /locus_tag="VCI33_000066"
     CDS             67917..68405
                     /gene="nrdI"
                     /locus_tag="VCI33_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP2831027.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQINNLITERTHPCHKPMRHT
                     SH"
     gene            68372..70552
                     /gene="nrdE"
                     /locus_tag="VCI33_000067"
     CDS             68372..70552
                     /gene="nrdE"
                     /locus_tag="VCI33_000067"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP2831028.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECQSCMI"
     gene            complement(70757..71509)
                     /locus_tag="VCI33_000068"
     CDS             complement(70757..71509)
                     /locus_tag="VCI33_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEP2831029.1"
                     /translation="MLKKRYQLAMILLLSCFSLVWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLVYPRAEQEAMAAYTCQQAAPINTSLDKTKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDQKKLHIEAYFKGSL"
     gene            71966..72418
                     /locus_tag="VCI33_000069"
     CDS             71966..72418
                     /locus_tag="VCI33_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831030.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMTRPVKAEVSEIGHDHVTIS
                     SNGQTDEGAAYGRGHDDGSKFGYEAGLQSSWNESEPPSSDKIPEPSVNPYESSNEQDR
                     EDYKEGFRDGYPGGYVAGWRKTHPIEATLQYLWYTVSSWFESLFNNSK"
     gene            complement(72725..73977)
                     /locus_tag="VCI33_000070"
                     /pseudo
     CDS             complement(72725..73977)
                     /locus_tag="VCI33_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            74433..74906
                     /locus_tag="VCI33_000071"
     CDS             74433..74906
                     /locus_tag="VCI33_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831031.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMGQPIKAEAVVSDLNDAVSS
                     MGDTAEETDVNIDGQEDEEDEEDDAYMQGRRLGEKEGYEAGQKDNAPRLFDSSTLEPP
                     TNPYEDAGDTLNSNWFKSGYSDAYGQAYEQGWNDTHPIQSTLIWLWGIVRNLFRF"
     gene            75159..75410
                     /locus_tag="VCI33_000072"
     CDS             75159..75410
                     /locus_tag="VCI33_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831032.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     SLVQNKGRQKVWSLAGLILAALAVAVVAYAYYDPTVIPY"
     gene            complement(75783..76283)
                     /locus_tag="VCI33_000073"
     CDS             complement(75783..76283)
                     /locus_tag="VCI33_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831033.1"
                     /translation="MKKTLTLLLALFTIGVTSSVRAEESSWVPLPRQQKVLDDNRNEI
                     PVHITGNKVTVTLPPGWSAWIDKNTKQRNSNSVTTTVTLDISTSQPPKEFTIYFQGDD
                     GFLAGMMTFIDSETLKNEQYKAFDEEYAENLFENSRLREEQKTWRDKVRDVFQDAWWN
                     FKGLFQ"
BASE COUNT        24091 a        14116 c        15780 g        22561 t
ORIGIN      
        1 caatagaaat tgactgaaaa ttcttagcac aaaaaatggt taagatatgt agctcattcg
       61 atttgacgaa tagatagaat tatattatta taaaaaacat gtaactttag taaggatgta
      121 acgtatgaaa aaaatatatg atttatgggt tagggttagt ctaatcaaaa aaataggtat
      181 cggtgttgta ataggagtca tgttgggcat tttggctcct gatcttacag gttttagtat
      241 tttaggaaag ttatttgttg gtggtttaaa ggctatcgca cctttgcttg tatttgcttt
      301 ggtttcccaa gccatctccc atcagaaaaa agggaaacag actaatatga cattaatcat
      361 tgttctgtat ttatttggta cctttgcgtc agctttagta gcagtactga cagcttatct
      421 attccctttg acgttagtgt taaatacacc tgtaaataca gaattatcac cacctcaagg
      481 tgttgctgaa gtttttcagt cacttctatt aaaattagtg gataatccga tcaatgcttt
      541 agctactgcc aattatattg gtgtgctatc atgggcaatt atttttggac tagcattaaa
      601 ggctgcaagt caagaaacaa agcatcttat aaaaacagcg gcagaagtta cttcacaaat
      661 agttgtttgg atcatcaatc tggcacctat tgggattatg agtctggtgt tcactacgat
      721 ttctgaaaac ggtgttggaa tcttatctga ttatgctttt ttgatactag ttttagtagg
      781 aacaatggtt tttgtcgcct tagtggttaa tccacttatt gcggttttga ttacacgaca
      841 aaatccttat ccccttgtct tgagatgttt acgtgagtca ggtcttacag catttttcac
      901 acgaagttct gcagccaata ttccagtcaa tatgcaatta tgccaaaaaa ttggattgag
      961 taaagatact tattcagtgt caattccact tggagcaact attaatatgg gtggggcagc
     1021 aattaccatc aatgtcctaa cgcttgctgc tgtacacact tttggtattc ctattgattt
     1081 tctaacagcc ctcttactta gcgttgttgc tgctgtctct gcttgtggtg cttctggtgt
     1141 tgctggggga tcgttgttac ttattcctgt agcatgtagt ttgtttggca tctcaaatga
     1201 tcttgcgatg caggtggtta gtgttggttt tattgttggt gttatccagg attcttgtga
     1261 gacagcctta aattcgtcaa ccgatgttct ttttacagct attgctgaaa atgctttttg
     1321 gaaacgtaaa aaagcctagc taggtttaac atagtaaaaa acatccttgg aaaacgccaa
     1381 ggatgttttt ttattattta agatagccaa gataatcaaa tttttcaaag gtgtggtcat
     1441 tagcgatggc tacaatgata gtattcggtt ctaaaggttc aaaaggtttg acattggtat
     1501 ctaaggtttt aacttctttt tgacgcatgc caatcacatt taactcatat ttacgacgaa
     1561 catctagttc ggaaagagat tgtccttccc agcttttagg aatgacaaat tcaatcatgg
     1621 aaatgccatg ttcgaggtag atgatgcttt cgatatgacg tcttaacaga tttgaagcta
     1681 cacgttttcc agaatcacgt tcaggggtga tgactttagt tgccccgatg ccatatagaa
     1741 cttcttcaaa aattttattt ttagccttgg caataatggt tggaacaccg agttttttac
     1801 agtgcatgac tgcaagaaca gaagattcca agttattccc tgaagcgata acgacagtat
     1861 cacaatgttc aatccctaca gctaatagaa attctttgtc agtaatatct ccaacagcgg
     1921 ctttagtgac caagtcggcc acctctttaa catgactttc tctgatatca atagcaataa
     1981 cgtcttgatc aaaattactt aattctctgg caacagtacg gccaaaaata ccaaggccaa
     2041 gaacgccgac agttttacgt tttaacatgt tttctcctta acccactaaa atatcagtag
     2101 tggcatattg aatggttttt tcttttcgtt gaatcagact gatgaggaca gtaattgggc
     2161 caacgcggcc gacgaacatc aagacaatga caataagacg tccagcagta gacaattgtg
     2221 gtgtcaaatc catagatacc ccaactgtcg cgatggcaga aatagattca aataagagtg
     2281 gtataggagc gatatgaggc tccacgctaa gtaataaaat aaaacctatc ataagaactg
     2341 caaaaaagaa gataagaact gtcatggtct gcttaattgt cttatttgcg ataattcggt
     2401 tacgaaaggt aacttctgat tgccctgaaa gttccgcttt aaagagtaag aaagtaatgg
     2461 cagcagtcgt cactttaata ccaccagctg ttccaccagg agcaccacca ataaccattt
     2521 gaatcatata aagaatgtta gtcggtgcta gggtatcgtt ataagagatt gttgcaaagc
     2581 cagctgttct catagtgact gtttgaaaaa atgaaaccat aagttgctga tgtaaactaa
     2641 aattggcaat ggttttgctg ttatcttttt ctaaaaacca ggttaagaag gttccaagaa
     2701 aaagaataac agctgtggtt tgtagtacta gacgagactg attggataat ttacgaaaag
     2761 ttgccccata acaatgaggt ctttcaaaaa agtatttttt aaaagctact cctaaatcaa
     2821 cccaaaccgc aaaaccaagt ccaccagaga taatcaagaa cgtaatgata acatttaatg
     2881 taggatttaa cataaagtct tttaaactag aagatcctaa attgtcaaaa ccagcgttac
     2941 aaaatgccga cacagctaga aaaatactat tgaagatacc atttttccac ccgaagcggg
     3001 ggataaagtc aatcatgata actatcgcag caaaggcttc tatactaaag gttactttat
     3061 aagcaaaaaa aaggtagtgc tttaaatctt tgctatcacc tcgatttaac gctgattgga
     3121 gcaaggtttg atcgctcaat cgcatttttc gcttgagggc aaaagtactc acagctatta
     3181 acgtcaccaa gccaagacca ccaatctgca ttagtgccat agcaatggtt tgtccgatac
     3241 cgttatacac ttcagcgacg ggaacaacag aaagtccagt cacacaaacc atggaaacca
     3301 cattaaagaa atgatcaaga tagacggtat taggaccatt ttgataatgg gtaaagggca
     3361 tggataacaa gagagtccca atcagaatca caatggcaaa actaaatgtt aagcgctgtg
     3421 tgactgaaag agatttgata aaagagcgtt tcatgtttcc tccagacata atatctccta
     3481 ctataataac aaaaataagt taaaaaagct aagtttcatc ttagcctttg gtactatttt
     3541 atagaggttt tttgttaggc aagccagctt ttcttggata cttgttaggt gtttcctttt
     3601 tcttctcaac aatggtgatg aagcgagaat ctccatttgg caattggtag ctatggtttt
     3661 taatgacttt accaaaaaga agacaaagag cattcttagc ttcttctaac tcttggtcag
     3721 cagcttgtgc ctttaaggcg attaattttc ctccaatttt taaaaagggg atggttaatt
     3781 cagatagaac ctgcatccga gcaaccgctc tggcagtaac gacatcaaat tgacctcgaa
     3841 aggccttgtc ctgtccgaaa tcttctgctc taccatgaaa aaaatggaca ttttccaagc
     3901 ccaattcctg agcaaggagt gtcagaaaag aaatccgctt attaagagaa tcaataatgg
     3961 taacctcaag gttcgggaaa agaattttca tgggtaggct aggaaaacca gctccagctc
     4021 caatgtccaa taatttgatt ggttcattag ctaaaaagcc ctgtaaaata ggagcgatgg
     4081 aatcataaaa atgcttgagg taaacctcat tttcttctgt aatagctgtt aaattaattt
     4141 tagtgttcca ttccactagt aatttaaagt aggtgtcaaa ttgttctttt tgtttcgatg
     4201 ataaggaaaa accatcttcc tctagtgttc ggtaaaagtc ttggggtgtc atttgtatcc
     4261 tctgtttttg atagttgtta ccattttatc ataaaatagc ccataacaaa atccaataaa
     4321 tcatcagaaa taacacctca ttacttgcta ttagcctaga aattgctata attgagacac
     4381 aaagattaaa ggagacccac tatgccaaca gttttgatta tcttagtcgt tttaggagtg
     4441 cttgcgctat ggttaatgat tagttacaat agcctggtta aatctcggat gcacacaaaa
     4501 gaagcttgga gtcagattga tgtgcaatta aagcgtcgta atgacttgat tccaaatctt
     4561 attgaaaccg taaagggata tgctagctac gagcaaaaga catttgaaaa aatcactgat
     4621 ttgcgtgcgc gtgttgcgaa tgcctcaact cctcaagaaa ccatggcggc ttctaacgaa
     4681 ttgagtaaac aagtgaccag tttgtttgcc gttgctgaaa attacccaga cttaaaagct
     4741 aacgaaaact tcttgaaatt acaagaagag ttgaccaata cggaaaataa aatctcatat
     4801 tctcgtcaac tctataattc aacaacgtct aattacaacc ttcaattaga atctttccca
     4861 agcaatatcg ctggtaaatt atttggtttt aaaccaagtg aattcttaca aacaccagaa
     4921 gctgaaaaag aagttccaaa agttgaattt aacttttaaa tatgaaatta tcatctagta
     4981 ggcaatcata agagaaagga gaattatgct ttatcaacag atttcacaaa ataagcaacg
     5041 aacagttgtt ttattagttg ttttcttcgc tctcttagca cttattggag cttcagcagg
     5101 ctatctgctg ttagataatt atgccatggg cttggttctt gctcttgtca ttggggtgat
     5161 ttatgctacc agtatgattt ttcaatccac cagtcttgta atgagtatga acaatgctag
     5221 agaagtcaca gaaaaagagg ctccaggctt cttccatatt gtcgaggata tggctatggt
     5281 ggctcagatt ccgatgccga gagtttttat tattgaagat ccttctttaa atgcttttgc
     5341 gacaggatct agccctcaaa atgctgctgt tgcagcaacg acaggtttac ttgaagtcat
     5401 gaatcgtgaa gaacttgaag gtgttatcgg ccatgaaatt agccacatcc gaaattatga
     5461 tatccgtatt tcgactattg ctgtagcttt ggcgagtgct gttaccgtta tttctagtat
     5521 cggtgggcgt atgctatggt atggtggcgg ttctcgcagg caaagagatg acggagatga
     5581 tgatgtttta agaatcatta cattactttt gtcgttatta tctttgcttt tagcgcccct
     5641 agtagcaagt ttgattcagt tggctatttc tcgtcaaaga gaatatctag ctgatgctag
     5701 ttctgttgaa ctaacaagaa atccccaagg tatgattaac gctcttgaaa aattgcagtt
     5761 atctcagcca atgaagcatc ctgttgatga tgctagtgca gccttgtata ttaatgagcc
     5821 tcgcaaaaaa aggagcttca gttcattatt cagcacccat cctcctattg aggagaggat
     5881 tgaaaggcta aaaaacatgt gagcgtttgg ggtttttgct caaacctcac ttttttatgt
     5941 ctttgggcag ataatctaaa atggcaggaa ttatcaaaaa tatctgaaca aaaagaaaaa
     6001 aatgagtgaa acaatggtta aaaatagaat gatttaaatc ttaaaattaa aaaacgtcag
     6061 aaaataaatg caaaactatt gacaaatctt ttagatagta gtaaaaataa atgagagttt
     6121 tattaggaga agatgatgtt agctatttcc gaaatcagaa aacacccaga cggtctctcg
     6181 tttgatcgtt tatgtgatgt caaaagtatg ttacttgagc gtgaccaaca gattattgac
     6241 ataaaggcag ttaaagctgt cggtaatgtt cgctatgata aggggcttta tctgttagat
     6301 tatcaattga gttacgaagt gatattaccg tctagccgtt ctatggttcc ggtctgttta
     6361 agtgaagtcc aacacattca agaattattt atagaggcga cagatttagc tgataagaaa
     6421 gagttggttg aagacaatct ggtattagtt ttagacaaag acgctattaa cctcgaagaa
     6481 agtattgtgg ataatatctt acttgctatt ccgctacagg tcttgacaga ggaagaaaag
     6541 aaatcaaaag agttgccagc aggtcaaaat tgggcagttt tgacagaaga agactaccag
     6601 tgtctaaaag aagaaaagca aaaagaaaat aatccttttg ctagcttgca agggttgttt
     6661 gatgaataaa actgaatatt aaagatgtct gtaattttta tttgaaaaaa cagctattct
     6721 ttgatatact ctttagagaa tatggttact tatctaaagt agaaggtatt ttgaccatag
     6781 agggcaaaga aggtaatgac tattagatgc ttcatatttt attcgctaga agactatttg
     6841 accattagaa ctgaaatagt ctaggatatg agatgaattt catagttttc tttctgggag
     6901 aaaaagatag attaagagga taagggttgg tataaatgac aaagaaaatt ttaattattg
     6961 aagatgaaaa gaatctggct agattcgttt ctcttgagct gcaacatgag ggttacgaag
     7021 tcattgttga ggtcaatggt cgtgaagggt tagaaactgc tttggaaaaa gagtttgatt
     7081 taatcctgct tgacttaatg ttaccagaga tggatggttt tgaagtgacc cgtcgtttgc
     7141 aaaccgaaaa aacaacgtat atcatgatga tgactgcgcg tgattctatt atggatgtgg
     7201 ttgcaggttt agaccgcggt gcagacgact atattgttaa accgtttgcc attgaagaac
     7261 tacttgcccg tattcgtgct attttccgcc gtcaagatat tgaatctgag aagaaagtgc
     7321 ctagtcaagg catttatcga gatctagttt taaatccaca aaaccgttca gttaatcgtg
     7381 gcgacgatga gatttctctc actaaacgtg aatatgattt gcttaatatt ttgatgacta
     7441 atatgaatcg tgtcatgaca cgtgaagaat tattgtcaaa tgtttggaaa tatgatgaag
     7501 ccgttgagac taatgttgta gatgtctata ttcgttatct ccgcggcaaa attgacattc
     7561 caggcaagga atcttatatc caaacagtgc gtggcatggg atacgttatt cgtgagaaat
     7621 aagtcatatg gaaaatcaga aacaaaaaca gaagaaatat aaaaattcgt taccaaaacg
     7681 actatctaat atcttttttg ttcttttttt ctgcattttc tctgccttta cactgattgc
     7741 ctatagttca acaaactatt tcttattgaa gaaagaaaag cagtcagtct ttcaagctgt
     7801 aaatattgtt agagttcgtc tttctgaggt ggactctaat tttacattag agaacttagc
     7861 agaagttttg tacaaaaacg ataaaacaca tctgagaatt gatgacagaa agggcagtcg
     7921 agtcattagg agtgagcgcg atatcacaaa tactctagat gcgaatcaag atatttatgt
     7981 ctataacatt gataagcaga tgatttttac cacagataac gaagaatcat ctcctggctt
     8041 gcatggtcct atcggtcggg tgtatcacga ccatatcgaa gatcagtatc gtgggttttc
     8101 catgacacaa aaggtatatt ctaatcggac tggaaaattt gtgggctatg ttcaagtctt
     8161 tcatgattta ggcaattatt atgtcattag agcaagactg ctgttttggc tactagttgt
     8221 tgagttattt ggcacaagct tagcctattt aatcatctta attactacgc ggcgcttttt
     8281 gaagccttta cacaatttac atggagtaat gcgtaatatc tctgaaaatc ctaataattt
     8341 aaacctgcgc tcagatattt cgtcaggaga tgaaatcgaa gaattgtctg ttatctttga
     8401 caatatgtta gacaaattgg agacacatac taagttgcaa tcacgtttta ttagtgatgt
     8461 cagccatgaa ttacgaacac cggttgcgat tattaaaggg catattggtc tcttacaacg
     8521 ttggggtaaa gatgacagtg atattcttga agaaagtttg acagcaacag cgcatgaggc
     8581 tgaccgtatg gcaatcatga ttaatgatat gttagacatg attcgcgtac aaggctcatt
     8641 tgagggacat caaaatgata tgacagtttt ggaagattct attgaaactg ttgttggtaa
     8701 ttttagagtt ttaagagaag attttatctt tacatggcag tcagaaaatc caaaaacgat
     8761 agcccgtatt tataaaaatc attttgagca ggctttgatg attcttattg ataatgctgt
     8821 gaagtattcc cgtaaagaaa agaaaatcgc gattaacctt tcagtgactg gcaaacaaga
     8881 agctattgtt agagttcaag ataaaggcga aggaatttct aaagaagata ttgaacatat
     8941 ctttgaacgc ttttatagaa cggataaatc acgtaatcga acaagtactc aggctggatt
     9001 agggattggc ttgtctattc tcaagcaaat tgtagatggg tatcatttac agatgaaggt
     9061 tgaaagtgaa ttaaatgaag gttcagtgtt tatcttacat attcctttgg cccagtctaa
     9121 agagagttag agtagcttaa ggcattgtta atgtgacagg tatagtatgt aatcattaga
     9181 taatagtagc agtcttttgc cacaactggt tagaagctga tgctattttt ttagcaatga
     9241 tggatgacat gaaagaaata ctgtcaatcg cgatgccttt tgttatcctt gataatatgg
     9301 taaaatagtg tattagatta atttgataga attggggtgt ttgcgtgcgt tgtccaaaat
     9361 gtaattacca taaatcgagt gttgttgaca gtagacaagc tgaagacggc aacaccattc
     9421 gtcgtcgaag agaatgtgaa cagtgtcata ctcgctttac tacctttgag cgtgttgaag
     9481 aactcccttt attagtcatc aaaaaagatg gcacacgaga gcaattttct agagataaaa
     9541 tattaaatgg ggttgtccaa agtgcgcaaa aacgacctgt ctcgagcaca gatattgaaa
     9601 atgtcatttc tcgtattgaa caagaagttc gaacaaccta cgaaaatgaa gtgtcaagta
     9661 ctgctattgg taatttagtg atggatgagt tggccgaact tgatgaaatc acatatgtac
     9721 gctttgctag tgtctacaag agcttcaagg acgttgatga aatcgaagaa cttttgcaac
     9781 aaattacgaa tcgcgtacgc ggaaagaaaa aacgtttaaa taatgatgaa accaattgat
     9841 acttttacat atctaaaacg caacaaagta actcttgatt cggtgacttt aatacagttg
     9901 tactttccaa ttattggaag tgacgcagtc tctatctatc aatactttat tcactttttt
     9961 gacgatggtt tacaacgtca taaattcagc gatattttaa atcacctaca gtttggtatg
    10021 aaacgttttg aagatgctct tgccatattg acagcaatgg aattagtgag tgtgtatcag
    10081 ctatcagata catatctcat tacgttgcac cagccgctta gccgtgactt gttttttcaa
    10141 catccagctt attctcgatt attagaacaa aagattggag aggtagctgt ctctgagttg
    10201 caggtaactg tcccaagtca agcaagaaat atttctaagc gattttcgga tatttttggg
    10261 gtgcaaggag atttgacgaa cgtacctcaa aagccacaaa agaattttga cctttcaagt
    10321 ttccagcaat taatggttcg tgatgggtta caatttgaag ataatcaaaa agatatcatc
    10381 agcctttaca gtattgctga acagtatgat atgacgtggt ttgatactta tcaaatagct
    10441 aaggcgacgg ctgttaatgg gaaaattcga cctgaacgct tattggctaa aaaaaatcaa
    10501 agcatgacaa aaccaagcaa agaaaatttt tcacaggctg agcaaattat tttgcgagaa
    10561 gcaaagcaag acagtgcttt agtctttttg gaaaaaatta aaaaggctag acgtgcaacg
    10621 attactaagg atgaacgcat tttattgcaa acattggcca aaatgaattt tcttgatgat
    10681 gttatcaatg tcatggtgct ttataccttt aataagacaa aatctgctaa tttacagaag
    10741 agttatgtct taaaaatggc caatgatttt gcctaccaaa aggtgtcaac ggctgaagaa
    10801 gctatagtgg ttttgcgagc cttcacggat cgtcaaagtc gtcggcaatc aaaagtaaaa
    10861 acaagtcaaa gcaatgtgcc taagtggagc aatcctgatt accaagagac aacaagtcaa
    10921 gaagagcagg cgaaactaga tcagttcaaa caggctgctt tgaagaggtt agaaaaccta
    10981 ggaaaagggg gtgactagat ggaaaagatt ggagaaacca tggcgaaatt gggtcaaaac
    11041 actcgtgtta atagcgacca gttgattcaa accattttag cggatcctga agtagcaagc
    11101 ttcatcagtc aacatcactt atctcaagag caaattaacc ttagcttatc taagttcaat
    11161 caatttttgg tggaacgcca gaagtatcaa ctcaaagatc cctcttatat tgctaagggc
    11221 tatcagccta ttctagctat gaatgagggc tacgctgatg tgtcttattt ggaaaccaaa
    11281 gaattggtag aagctcaaaa acaggcagct atctctgaac ggattcaact ggtcagtttg
    11341 ccaaaatcct atcgccatat tcatttacca gatattgatg ttaataatgc gagccgtatg
    11401 gaagctttct ctgctatttt agattttgtg gaacaatacc catcagcaga gcaaaaaggc
    11461 ttatatttgt acggagatat gggaattggc aagtcttact tgttggctgc tatggcccat
    11521 gaattgtctg agaaaaaagg tgtgtcaacg acccttctac attttccgag ctttgccatt
    11581 gatgtaaaaa atgccatttc aaacggctct gttaaagaag aaatcgatgc ggtcaaaaat
    11641 gtaccagtgc tcattttaga tgatattggt gcagagcaag ccacttcttg ggtccgtgat
    11701 gaggtgttgc aggtgatttt acaataccgt atgttagagg aattgccaac cttctttacc
    11761 tctaattaca gctttgcgga tttggagcga aaatgggcaa ccatcaaagg aagtgacgaa
    11821 acttggcagg ccaaacgtgt catggaacgc gtgcgttatt tggctagaga gtttcattta
    11881 gaaggggcta accgccgtta agtttgagat agcaatcaag aagaatagct acacgtatga
    11941 gtgagtgaaa gaaaaggaga atcatggttt tacctaccgt tgccattgtg ggccgtccaa
    12001 atgtgggcaa gtccacctta tttaatcgaa tcgcaggaga gcgcatttca attgtagaag
    12061 acgttgaagg ggttactcgt gaccgtattt atgctactgg ggagtggctt aaccgccaat
    12121 tctcattgat tgatacaggt ggtattgatg acgttgatgc cccgtttatg gagcaaatca
    12181 agcaccaggc tcaaattgct atggaagaag cggacgttat tgtctttgtc gtttctggta
    12241 aagaaggggt gaccgacgct gacgaatatg tgtctaaaat tctgtatcga accaataccc
    12301 cagtcatttt agctgttaat aaggttgata atcctgaaat gcgtaatgac atttatgatt
    12361 tttattcact tggtttaggg gatccctatc cagtttcttc tgttcatggt atcggaacag
    12421 gggatgtctt ggatgagatc gttgaaaact tgccagtaga agaggctgaa gaaaatgatg
    12481 atattattcg cttcagttta atcggtcgtc ctaatgttgg gaaatctagc ttgattaacg
    12541 ctattttagg tgaggaccgt gttatcgcta gccctgtcgc agggacaaca cgtgacgcta
    12601 ttgacacgca ttttacggat gctgacggtc aagaatttac catgattgac acggctggaa
    12661 tgcgcaaatc tggcaagatt tacgaaaata cagagaaata ttctgtcatg cgtgccatgc
    12721 gtgccattga ccgctccgat gtggtcttga tggttatcaa tgctgaagaa ggtatccgtg
    12781 aatacgacaa gcgtatcgct ggctttgcac acgaagctgg taaaggaatg attatcgtgg
    12841 ttaacaaatg ggataccatt gacaaggata atcataccgt tgctaagtgg gaagccgata
    12901 ttcgtgacca attccaattt ttgacatacg ctccgattat ttttgtgtca gctttgacga
    12961 agcagcgcct caacaaattg cctgacttga ttaaacgtat cagtgagagt cagaataaac
    13021 gtatcccatc tgctgtcctc aatgatgtta tcatggatgc tattgctatt aatccaacgc
    13081 caacggataa gggtaaacgt ttgaaaattt tctatgccac tcaagtgtca gtaaagccac
    13141 caacatttgt cgtctttgtt aacgaagaag aattgatgca cttctcttat ctacgcttct
    13201 tggaaaacca gattcgcgca gcctttacct ttgaagggac accgattcat ctgattgctc
    13261 gcaaacggaa ataagaaatg aacatcaact aaaatgctga cctaagaaat aatctcatca
    13321 ttgataagga ctgtgttgtg tatgttacag gagacagtcc tttttataat acctttaagg
    13381 gtgtagacgg aagattagta gcaaacaaag aaaaaggatt ttgacaagaa atatcagtcg
    13441 gtttttgcta taatgaaagg ataagcaaaa aggtggaaaa atgatggcta gattaattcc
    13501 aggacgagta aggaatgaag gtattaagct ttatgagcaa ggtttagtct cattccaaga
    13561 tgataataaa ggcattcttc aaatagaagt ggagacttat caggttcaat atggagcaga
    13621 tgatgaggat atcacttgtc aatgcgacac atttcatatg aaacattatt gcaagcatat
    13681 tgcagcagtc gagtattttt taaaaaatga tcaaaaaggt aagctttttt taaaacagtt
    13741 aacaaatcaa acaaaaatca aagaaacaac aaagaaaatg acatcttttg gtagtctctt
    13801 tttagatagc ttagccatga atgaagatga ttctgttaaa tacaggctgt ctgctcttgg
    13861 tagtcgaagc cctttttcgt cagattactg gtggagtctg aaaatcaatc gtttaccaga
    13921 tgatcgttct tatgtgattc gagatattaa aggctttttg cagcttatta aaaaagaagg
    13981 cttttatcag attggaaaaa attattttga acaactttca tggctgcagt ttgacccatc
    14041 cagtcaagac cttattgagt ttttatggcg cttagcatct aatactgaca aaggggacaa
    14101 cgagaacatt tttccaaatc atgcaaggca tttgcgctta cctagtggtt tttttgaaga
    14161 aggtattcac tatttgacgt cactttatga ctttactttt gaggggcctt ctcaaactta
    14221 tcatcatctt tttgtccgtt ctttggaagc agaggcaggc ctttatgagt ttaaagtgga
    14281 agtgcatcgc aaatctattg aattgcaaat tgctgaaaaa aatgtgcaat acctctttga
    14341 taatgactat cttctttatc aagatacctt ttatcacttg acgttaaagc aacgaaaaat
    14401 ggttcaggct attcgtagtc tgcccattga ggccgacttg gctaaacata ttcattttga
    14461 tttggatgat catgcgaaat tagctgcaag cttatcagat tttaaacaga tcggtctagt
    14521 agaggctcct aaaagctttg ctattagaga ctttgaggtg acttttcaat ttgatttgtt
    14581 aaatcgagat gaaatcagtt gtcagcttat gtttgattat ggcaattacc aagttagtga
    14641 caaagcaagt ttagaagcac ttccttttgc tagtcattta aaaaaagaag aaaaaatcaa
    14701 tcgttcttta cttgcttttg gcttctcgcc acaattttac tcaaaaaagc gactaacaag
    14761 tgcaaaagag ctctatactt tttttgaaga aacagttcca tgttttgagc gcttaggtaa
    14821 tgttgcgttg agtacagcta tccaagcatt acaagtgaaa gaaatgccca agatcgctat
    14881 tagacgcaat cagggattac tagatatttc gtttgatttt tcaaccatta ttgaaaatga
    14941 tattgaccaa gctgttacag ctctttttca aaataatcct tattttgtga gtcaaacagg
    15001 tcaattagtg gtttttgatg atgaaactca aaaagttagc aaaagtttac aagaattaag
    15061 agcaagacag ctaaaaaatg gtcacttgca actggatggc attagagctc tacaagtatc
    15121 caaacttttt gaaggcatga catctgttca tttttcaaag gaattagaag agttagccta
    15181 tcatttgcag catcctgaaa ctttttcaat aaaaccgtta ccagttaagg ctcaaatgcg
    15241 agattatcaa cgcaacggtg ttcagtggtt atcaatgctc aatcattatg gctttggtgg
    15301 cattttagca gatgacatgg gacttggaaa aactctccag acacttgctt ttttagctag
    15361 tcacctgaaa tcagatagta aagtgctcat tttatctcca tctagtctta tctataattg
    15421 gtttgatgaa tgtcaaaaat tcacacctca gctagatgtt gtagtttctt acggacttaa
    15481 acaaatcaga gatcaaatca tcgaagaagg tcatcaaata accataacca gctattcctc
    15541 ctttagacag gattttgaga cctatcaagc gtttcactac gattatctta tcttagatga
    15601 agctcaagtg ataaaaaatg cgcaaactaa gatttcacac tgtttacgtg cgtttaatac
    15661 agccaattgt tttgcgcttt cgggcactcc tattgaaaat aaaatgcttg aaatttggtc
    15721 cattttccaa attgttttgc cagggttgct accgactaaa aaagagtttt taaaattaac
    15781 agctgagcaa gtatcgcgct atatcaaacc ctttgtgatg cgtcgtaaaa aagaagatgt
    15841 cctaccagaa ttacctgatt taattgaaac taattattca aacgagatga cagatgaaca
    15901 aaaagctatt tacttagcgc agttaaggca gatgaaagat caaattcgaa attctagtga
    15961 tgtggatatt agccgtcaaa aaattgagat tttatctggt atcactcgat tgaggcaaat
    16021 ctgtgacact ccaagtctct ttatggatta ccaaggcaaa agtggtaagc tagatagttt
    16081 gcgaatctta ttgacacaaa ttaaagaaaa tggtcatcgg gctttgattt tttctcaatt
    16141 tagagggatg ctggacttgg ccaagcaaga gatgactgct ctaggattga cctcttatca
    16201 aatgaccgga tctacccctg ccaatgaacg tcaagaaatg actagagcct ttaataacgg
    16261 ttccaaggat gcttttttga tttccctcaa ggcaggtgga gttggaatca accttacggg
    16321 agcagacaca gtgattttaa tcgatttatg gtggaatcct gctgttgaaa tgcaggcaat
    16381 cagtcgcgcc taccgtattg gtcaaaaaga aaacgttgaa gtatatcgct taatcactcg
    16441 agggacgatt gaagaaaaga ttttagagct tcaagaaagt aagcgcaatt tggtgactac
    16501 agttttggat ggcaatgaaa accgagcaag tatgtccatt gaagaaataa aagaaatttt
    16561 ggggctaaac actcattaat cttccttgat tttagtgaca ttaaaaaccc tttaaagaag
    16621 atgacaccaa aagcacctta tataacctcg ttaaaacgct tctttttgaa atatctttat
    16681 tgatatagcg attgtcattt gctagtaatg attaaaacat ttagaaaaag acaagtcata
    16741 gaatcatcaa ggaatgcttt gaaaaataac agtatttaag ttataataga ggatgctaca
    16801 agaagggaga atcaaaaaag gatggtaaaa aatcagtttc ctttagttgc agatggcatt
    16861 gctattagtg atccagcaaa gcaaatggct ctgtatgaaa atgaagatct cattaccaac
    16921 atccgtggct attaccagga caaggagtat gatgatattg ctagaaatga ggagtttact
    16981 gctaaagcaa cttctcggca ggccccttct agtaagcgct tttgtagcaa tgatgaaaaa
    17041 caccattatg tcaaagaggc gcgtcaaaaa gcaaagcaag atctcaagga aaaaagacag
    17101 gcttatcttg ccaaagaaat ggcctatgtt cctaaacagg tttctaaaaa gcaacagcct
    17161 gcggattctt ctccttctca aaagcaagca acaacagaaa tgagtcgttt tacaaaaaag
    17221 cttcatcaag acaactatat tttagctgag ttgccaaaag aatataaaga gccaaaaaat
    17281 cttcctcaac aaggaactac aaaaaaaaat aattacgatt ttttaaaatc cagtcaaatt
    17341 tacaataata aagaaatgag acaacaacga gaaaaaacaa ttgcgcaaga gcttaatctt
    17401 agtaggtttg aggatcttcc ttaataaatc tactcagcgt atgcagatga caaaggagtt
    17461 aagatgtcaa aaacgtatca ttttatcgga attaaagggt caggaatgag tgccttggcc
    17521 cttatgttgc atcaaatggg gcataaggtg caaggtagtg atgttgaaaa atactatttc
    17581 actcaaagag gattggaaca agcgggcatc actatcttac cttttagcga ggacaacatt
    17641 acgccagata tggaacttat cgttgggaat gcctttcgtg aaaacaacaa agaagtggcc
    17701 tatgctttga gacaccagat tcctttcaaa cgttatcatg actttttagg tgattttatg
    17761 aaatcattta tcagttttgc agtagcaggt gctcatggta aaacttctac aacgggttta
    17821 ttgtcacatg tcttgaaaaa tatcacagac acatcttatt taattggtga tggtactggg
    17881 cgtggatctg ctaatgcaca gtattttgtt tttgaatcag acgaatacga acgccatttt
    17941 atgccttatc acccagaata ctctatcatt actaatattg attttgacca tcctgattat
    18001 ttcacaggaa ttgctgatgt gcgaaacgct ttcaacgatt atgctaagca ggtcaagaaa
    18061 gctttgtttg tttatggtga agatgacgaa ctaaaaaaga tcgaagcacc tgcacctatt
    18121 tactattatg gttttgaaga gggcaatgat tttatcgctt acgacattac gcgtactacg
    18181 aacggatcgg actttaaggt aaaacaccaa ggagaggtca ttggtcagtt tcacgtacct
    18241 gcttatggaa aacataacat tttaaacgct acagcagtaa ttgcaaactt gtttgtagcc
    18301 ggcattgata cggctctagt ggcagaccat cttaaaactt tttcaggtgt gaaacgtcgt
    18361 tttacagaga aaattatcaa tgacactatt attattgatg actttgctca tcatccaacc
    18421 gaaattgtgg caacaattga tgcggctaga caaaaatacc caagtaaaga aattgtagca
    18481 atatttcagc cacatacttt tactcgcact attgccttat tagaggattt tgcttgtgct
    18541 ttaaatgaag ctgacagtgt ttatttagcc cagatttatg gttctgcacg tgaagtagat
    18601 aaaggtgaag ttaaggttga agatttggct gctaagatta tcaaaccatc acaagtagta
    18661 acggtggaaa atgtctctcc tcttcttgat cacgataatg ctgtttatgt ttttatggga
    18721 gctggagata ttcagctata tgaacattct tttgaggaat tacttgcaaa tttaactaaa
    18781 aataaccaat aacatgtcct gccttaggca ggttttctca ttctaaagga gtttgatatg
    18841 ttgattagac aggttcaagg atccgatttg gaagttattg ctaccattga aagtgacaat
    18901 ttttcaccac aagaagcaac gacaagagca gttcttgaag agcgcatacg gctgatacca
    18961 gatacttttt tagtggcact tatcgatcag gaaattgttg gctatatcga aggtccagtt
    19021 gtcactactc ctattttgga ggattctttg ttccacgggg tgactaaaaa tcctaaaact
    19081 ggaggctata ttgctattac tagtttgtcc atagctaaac attttcagca acaaggggtt
    19141 gggacagctt tgttggcggc actaaaagat ttagtagtag cgcagcaaag aacaggtctt
    19201 attttgactt gtcatgatta cttgatttcc tattatgaaa tgaatgggtt tattaaccaa
    19261 ggaatttcag agtctcaaca tggtggaacc ctttggtacc aaatgatttg gaaactttag
    19321 aatcacagct gattcaactg ttggtttgtt aaaaataagt aacaaatgca tcatttaatt
    19381 gcaattagga tacttttaag ttataatggc ggttgattat atttaggagg attggctttg
    19441 accgatttta aggataaaga ccaacaagat cagcagcgaa gctttaaaga gcagattctt
    19501 gctgagttgg aaaaagcaaa tcaaatcaga aaagaaaaag aggaagaact ttttcaaaaa
    19561 gagttggaag ctaaagaagc agctaggaga acggcccagc tatatgctga atataaaaga
    19621 caagatgctt ttcaaaaaga gtctatagca cataacaata agacagctaa acactttcaa
    19681 gctataaaag gtgcggtaat gacttcagaa gcgcttaaac cgactttact ttctgaaaaa
    19741 gaaaactcat ctttaaaaac gacaaataag agagtcgtgc aggcaaatga gcttcaagag
    19801 actgcctcta aagaatctca agtaccgtta actattgaga aaggtcattc agtgagacga
    19861 aaattaagca aacgccaaca gactgagcga gctgctaaaa agatttcaac cgttttgatt
    19921 agttctatta ttataaccct tttggctgtt actctagcag gagcaggcta tgtttatagt
    19981 gctttaaatc ctgttgataa aaatagtgat gcctttgttc aagttgagat accatctggg
    20041 tcaggcaata aattgattgg tcaaattctt caaaaaaaag gtttaatcaa gaatagcact
    20101 gtttttagtt tttatacaaa atttaaaaac tttacaaatt ttcagagcgg gtattacaat
    20161 ctgcaaaaaa gtatgagtct agaagaaatt gctagtgctt tacaagaagg tggtacagca
    20221 gaacctacca agccatctct tgggaagatc ttgattccag aaggatacac gattaaacaa
    20281 atagctaaag ctgttgagca taatagcaag ggaaagacca aaaaagctaa aacacctttt
    20341 aacgagaagg attttttgga tttagtcaca gatgaggctt ttattcaaga tatggtaaaa
    20401 agatatccaa aattattagc aactatccca actaaagaaa aagctattta ccgtttggaa
    20461 ggttaccttt tcccagcaac ctataactat tacaaagaaa ctaccatgag agaacttgta
    20521 gaggacatgc tggcagctat ggatgctact ttggtaccct attatgataa aattgctgct
    20581 agtggtaaga cagtcaacga ggtattgacc ttggcctctt tggttgaaaa agaaggttca
    20641 acagacgatg acagacgtca aattgcaagt gtcttttata accgccttaa tagcggaatg
    20701 gcactacaat ctaatatagc tattttgtat gcgatgggga aacttggtga gaaaacaacc
    20761 ttggctgagg atgctactat tgacaccacc attaattctc cttataatat ttataccaat
    20821 acaggtctga tgcctggtcc agttgctagc tcgggggttt ctgcaattga agcaacccta
    20881 aatccagcct caacggatta tttatacttt gtggccaatg tccatactgg tgaagtttac
    20941 tatgcaaaaa catttgaaga acactctgca aatgttgaaa aatatgtgaa tagtcaaatt
    21001 cagtaaacac aaacatggtg cttaagcacc atgtttgtgt ttctgattcg ttcaaaaaag
    21061 aaaagagaga aaacaattat ggcagaaaaa acgtatccga tgaccctaac ggaaaaagaa
    21121 caacttgaaa aagaacttga agaattaaaa cttgtgcgtc gtccagaaat agtggagcgt
    21181 attaaaattg cacgttcgta tggagatctt tcagagaact ctgaatacga tgccgcaaaa
    21241 gatgagcaag cttttgttga aggtcaaatt tcaacgcttg aaactaagat tcgctatgct
    21301 gaaatcattg acagtgatgc tgttgcaaaa gatgaagtcg ctatcggaaa aacagttatc
    21361 gttcaagaag ttggaacaac agataaagat acctatcata ttgtaggtgc tgctggagca
    21421 gatatcttct caggaaaaat ttcaaatgaa agcccgattg ctcaagcttt gattggtaaa
    21481 aaaacaggtg acaaggttag aattgaatca ccagcagcta cttatgatgt tgaaatcatc
    21541 agcgttgaaa agacaaatta atcagatgaa gccattattt gcctaaccac taagtgtttt
    21601 ggttgctaat ggcttttcta gtatcttcaa atgaggttga ctgttaggaa ggtcaagtat
    21661 taatcgcatt gattatgagc caagaggact atagatcaaa gatcgttttc gatggttatt
    21721 taatgccaaa agaaaagacc agcaccttaa tgtactgacc ccaaaaagtt ggacactata
    21781 ttttaaggaa gtgtatgatc taaggaaact gacactaatg attgaccgtt ttgcaagact
    21841 ttattaatga tttcttgctt tattttaaat gaataagtct gatacgtttc cttccttaac
    21901 actatcgact ccatactgac ccatcagtca aacggtatat ctcaaacgac tacggtttac
    21961 tccaaattga ttggctagtt gaggccaaga aacacctgat tttttttaat gctaaatttc
    22021 tagcttatct tcatagctta atttcataca aaaacacccc aatcgttaga tttttttgat
    22081 atgtcccctt tcactagatg agtccagtaa aagggggcat atcgtttttt gtctaacttt
    22141 tggggtgcag ttcataattg tgctaggttt tttgtctttc ttaacgcttt cgctgttttc
    22201 cagcattacg attggatttt atttttttag gcaagttagc ctgttccatg ttttgtgaag
    22261 gcgtaacgtc cttacgagaa gaagttgatt ggtatgcttt aggcgggttt ttagcgtatt
    22321 cttctttaat ttgcttatgc aatcgaggtt ttaagaggta ggttgtgatt aactgttgaa
    22381 taatactaaa gaaaccacca actagccagt aaagaccaac accagcaggg agtgagaagg
    22441 acatgaagat catcatgata ggcatggtgt acatcatcgt cttcatttgc tcacgttgtt
    22501 cttctgaaac ggccatcatt gataaccatg attggaagaa gtaaagagca gcgataattg
    22561 ctgttaacac taaactacgg ctaccaagat caatacccat aaaggtgctt gttgatactc
    22621 ctttagtgta ttgagcggca aaatacatgg cagaaaagaa tggcatctgg atgagaagag
    22681 gtagacatcc aatacctcca agaggattaa tcccatgagc acgctgagca gccattaatt
    22741 ctgtttgggc agccattttt tcttcctgac tacttgcctg tttgatacgt ttgttgattg
    22801 gttcaaaaac aggtttcaag aaggccattt tctcagattg ataacttgct ttccaagatt
    22861 gatatagacc tagtggtaag ataagtgttc taacaataat ggttacgatg atgatagcca
    22921 gtccataacc tagtccagca ttgtttgcaa agtaatcgat aaagtatgac atgggttttc
    22981 caagaaattc ccaaatcata cctttgggat ttccgtgagc gtctcttccc acacagcctg
    23041 tcaaggtgaa tagaattgac agtgctagtc ctgaaaagag gatgcgattt aatgttagtt
    23101 tcaatgtgtt tatcctttaa ttttaatata cttatctatt ttacagtttt tttcagaaat
    23161 tcacaatagt tctgaaggcc gatttttact tgcctatttg aaagtcctga aagtcttcaa
    23221 aattagccat ggtgacatcc acataagtga cttttgccca tttggatggt cctttacgaa
    23281 cttcttgaat aaatgtggct attttatctg aatcttttga ttgggccagg atttcaacgg
    23341 tcccatcgtt attattccag acacgaccat aaatatctcc tatatctaga gctaaagtat
    23401 gggtagcgta tcgaaaacca acaccttgta cacgtccaga aacaattaaa cgtacttttt
    23461 gcataagaag ggcctccttg cttttattat agcacatact tagcaggaaa tctctttagc
    23521 taattgtcta cttcctgtca cttttactaa agacaagaat acaaaaatga gatagttagt
    23581 agtactaaca gtaaaagagg tttgtctatt ttaaaaatag tatcaaaaaa aggtctttta
    23641 ggtttaaaac attgaactgc ccccaaattg accatctaag aagttgatgc tttcaaatgc
    23701 tgtcgcgtat ggtataatag gtgcatggtt attacatcaa aaacaaatca actaattaag
    23761 caaacaaaaa aattattgca aaaaaagcat cgaaaacagt cctatttgat tgagggctgg
    23821 cacctttttg aagaagccca aaaatcagga caagtttttc gccacatctt tgttttagag
    23881 gaaatggtgg agcgtctagc aggtgagcaa gaactcgtta ttgttagccc acaagtgctc
    23941 aaggagttga ccgactctcc tagtccacaa ggcattgttg ctgaggtgga gatacctaag
    24001 ttagcctttc catcagatta caaagggaaa tatttggttt tagaggacgt ccaagaccca
    24061 ggaaatcttg ggaccatcat tcgtacagca gacgctgcac ggtttgatgg ggtctttctt
    24121 tctgaaaaat cagcagatat ttacaaccaa aaaaccctac gttccatgca aggtagccat
    24181 tttcatttac caatttggcg aacagatgtc tatcagcttt gtcgagagct ccaagagtat
    24241 aaaactccca tcctagcaac aactctgtca aagaaatctg ttgactataa aagcctcaca
    24301 catcatgaaa ggttggcttt ggtgttggga aatgaaggcc agggcattag tgctgaaatg
    24361 gcagccctag cagaccaact ggttcatatt acaatgcctg gtcaggcgga aagtttgaat
    24421 gttgccgtag cagcaggaat cctcattttc agcttgattt aagctaatat gatataataa
    24481 ccaagtgggg tgattatgtt atgcattata cagaagataa agaatatatg gaacacgtcg
    24541 gtcatttgat cgctcatcct cgctttcaaa aattgagtca tattgttcag caccaacatt
    24601 caacgcgtct tgaacattct atcaatgttt cttattccag ttataaatta gccaagcgat
    24661 ttggttggga tgctaaaagt acagccagag ggggcttact tcatgatttc ttttattatg
    24721 attggcgcgt gaccaagttt aataagggtc atgcctgggt tcatcctcgt attgctgtac
    24781 gaaatgcgaa aaagttaaca gaactcaata aaaaagaaga ggatatcatt ctaaaacaca
    24841 tgtggggagc gaccattgct tttccgcgtt acaaggaaag ttatattgtg acaatggtag
    24901 ataaatactg ggctgtcaag gaagcagtga cacctttaag acaaaaatgg tctaatcgac
    24961 ggtttcttcg ccgcaagacc ctccaaagtc ataatcgtta agtaaaggag ttattatgaa
    25021 cgatcacgtt atttatacac aatcagatgt gggattaaat cagttttttg ctaaaattta
    25081 tagtctagtg ggaatgggag tgggtttatc tgcttttgtt tcttatttga tgctttatcc
    25141 atttagagaa aatttaatct ctattcttgt taatcagccg atgatttact atggtgctgc
    25201 gattattgaa cttattttag tttttgtagc cagtggtgca gcacgaaaaa acacaccagc
    25261 tgcattgccc atttttctta tctattctgc gctaaatggc tttaccctaa gctttattat
    25321 tgtggcatac gctcaaacga cagtttttca agcatttctt tcttcagcag ctgtgttttt
    25381 tgccatgtca atcattggtg tcaagactaa acgggatatg tcaggtctta gaaaggctat
    25441 gtttgctgct ttgattggtg ttgttgtggc tagtctgatt aatcttttta ttggtagtgg
    25501 gatgatgagt tatgtcatta gtgtgatttc cgtgctaatt ttctcaggtc tcatcgcatc
    25561 agataatcaa atgattaagc gcgtttatca agcaacaaat ggccaggttg gtgatggctg
    25621 ggctgttgct atggccttaa gcttgtattt agattttatt aacttattta tcagccttct
    25681 tcgtattttt ggaagaaatg attaacatta aaaagtgagt ataactactc actttttaat
    25741 gttaacagga taagtaaact gtaaggaaga aattcgagat agatataaga tgaggctgct
    25801 aaaagaccta gtgttaattt gtaaactctt tctttttttg gtatactatt aaggattaaa
    25861 tacgaggaga atatagatag atatgtcaac agctatttgg attttattac ttattgttgc
    25921 ccttggggta ggtgtttttg gtgggatttt tattgcccgt aaacaaattg aaaaagaaat
    25981 tggtgaacac ccacgtttaa caccagaagc tatccgtgaa atgatgagcc agatggggca
    26041 aaaaccaagt gaagcaaaaa tccaacaaac ttatcgtaat atcatcaaac aatcaaaagc
    26101 agccgtatcc aaaggtaaaa aataaaagct aattgttata ggggaaacct ttgtgatagt
    26161 tagaagtgaa cagtctttga tctcaatgtt cagtagcact tgtcttatca gttagatttg
    26221 tcaactgttc agggtttaaa aagaaactca gttggctatt tccaactgag tttttcatat
    26281 cgtcagtaag gaagtaaaca taatggatac aagaccaatt gggtttttag attctggtgt
    26341 gggaggtctg accgtcgttt gtgaattgat tcgtcagctc cctcatgaga agattgtata
    26401 tattggtgat tcagcaagag ccccctatgg tcctagaccc aaaaaacaaa ttaaggagta
    26461 tacttgggag ctagtcaatt ttttgctgac tcaaaatgtt aaaatgattg tttttgcatg
    26521 caataccgca acagcggtgg cttgggaaga agtaaaagca gctttagata ttcctgtttt
    26581 aggggttgtc ttaccggggg caagcgcagc tattaaatca acgacaaaag gccaggttgg
    26641 ggtcatcgga accccaatga cagtggcttc agacatttat cgcaaaaaaa tccagctatt
    26701 agcaccatct gttcaagtaa ggagtcttgc ttgcccgaag tttgtaccga ttgtggaatc
    26761 aaatgagatg tgttcgagta tagctaaaaa aatagtttat gacagtctat caccattagt
    26821 cggtaaaata gatacccttg tactaggatg tactcactat cccttgttac gaccaattat
    26881 ccaaaatgtt atggggccat ctgttaagct gattgacagt ggagcagaat gcgtccgaga
    26941 tatctctgtc ttattaaact attttgacat taacggtaat tatcatcaaa aagctgtcaa
    27001 acaccgcttt tttacaacag caaatcctga gatttttcaa gaaattgcct cgatttggct
    27061 gaaacaaaaa ataaatgtgg agcatgtcac cttatgagtg aaaaaattta cgaatacaaa
    27121 gatgaaaata actggtttat cggaaagatg actggtcata atttaatatc gggctgggga
    27181 gtcaaacaca cgacaatcaa aaaaatagat gaccttttag acggtattgc cgcaacgtta
    27241 gattgggaaa atcctaaagg ttacgatgtt tcggtggtgc gttatcagtc tcctttatct
    27301 ctcatcacct ttattatcga catgataaat caagagaccc agcgtaagat taaggtaact
    27361 ccccatgcag gcacgatttt attaatggaa aatgcgaaac tgcttgcagt ttatctccca
    27421 gaaggtggag tttcaacggc tacatttttt gcgactagtg aacagggatt tggggatatt
    27481 attttaatcg ctacacgaaa cgagggaaaa accaaagagt tccgcaatct gtttggccag
    27541 ctaggttatc gtgtggaaaa tctcaatgat tatccagaat tgccagaagt agcagaaact
    27601 ggtactactt ttgaagaaaa tgctcgcttg aaagcagaaa cgatttcacg cctcacagga
    27661 aaaatggtat tagcagacga ttcaggacta aaagttgatg ccttgggtgg tttacctggg
    27721 gtttggtcag ctcgtttctc aggaccagat gccacagatg ccaagaataa tgccaaactc
    27781 ttacacgaat tagctatggt gtttgaccaa aagaagcgct cagctcaatt tcatacgact
    27841 ttggtagtgg ctgctcccaa taaagatagt ttggttgtgg aagccgagtg gccaggctat
    27901 attgctacgc agcctaaggg agaaaatggt tttgggtatg accctgtctt tatcgtcgga
    27961 gagactgggc gtcatgctgc agaattagag gctgaccaaa agaatcagtt gtcgcataga
    28021 ggtcaagcag ttcgaaaatt aatggaggtc tttccagcat ggcaagcaaa acaatcatag
    28081 taatgagtga ctctcatggt gaccgagata tcgttcaagc cattaaagac aagtatcttg
    28141 gtcaagttga tgctattttc cataatgggg attctgaatt aaacagctca gatcctattt
    28201 gggctggtat ttatgtcgtt ggcggtaatt gtgactatga tacaggttac cctgatcgtt
    28261 tagtaactca attaggcaca gttacgattg cccagacaca tggtcacttg taccatatta
    28321 atttcacttg ggataaactg gactactttg ctcaagaagt agcagcagat atttgtctat
    28381 atggacacct acaccgtcca gctgcttggc aagttgggca aaccctcttt atgaatcccg
    28441 gatcagtaac acaacctcgc ggagaaatta acgagaaatt atatgctcgt gttgaactaa
    28501 ctgatactca aattaaagtt gattacttta caagagatca taaactttac ccatcactgt
    28561 ctaaggaatt taaacgatga tcgcaaaaga atttgaaacg tttttaatga gtcatttaga
    28621 taattatctt atcccggaac aagacttggc tatttttatc gacacacata atgcagacca
    28681 tgtgatgtta cttttggtga gcaatggttt ttctagagtt cctgttatca cacgggagaa
    28741 aaaatatgtg ggaaccatta gcatctcaga tattatgatg taccaatcta aaaggcagct
    28801 gacagattgg gaaatgagtc aaacagatat tggcgagatg gttaatacca aaattgaaac
    28861 cattagcatt acttccagcc taacagaaat tatgcacaag ttaattgatt tcccattctt
    28921 accggttgta gatagagcga atcgctttgt tgggattatt acgcgaaaat ctatattaaa
    28981 ggctgtcaac agtcttttac atgattttac agatgattac acgattataa aaaaatgaag
    29041 agttatattg aaccttttat tgctagcaag gctttatctc aaaatagtca aaaagcttat
    29101 cgatacgatt tgcaacagtt ttgtcaactg attggtgaac gtgtcaatca agacaaacta
    29161 ttactttatc aaaatagtat agctaacctc agtttatctg caaaaaaacg caaattatca
    29221 acagctaatc aattcttata ctacttgtat caaataaagt atttaaatag ctattttcga
    29281 ttaacagaca ccatgaaagt gatgcggact gagaagcagc aagctgctat tattaatact
    29341 gatattttct atcaaaaaac cccttttgtg tgggggcaat taatcagctt attaatattg
    29401 gagctgggtt taactccgag tgaagtagct ggtattgaag tagctaactt ggatttaaat
    29461 tttcaaatgt tgacgctaaa gactaaaaaa ggtgtgcgtg tattaccttt gtcgcaaata
    29521 ctaatccctt ttttagaaca acaactcgtt ggcaaagaag cctatctttt tgaacatcga
    29581 ggcatccctt tttcaagaca atggtttttt aatcacctaa aaacctttgt tcgaagtatt
    29641 ggttatgaag gattaactgc tcaaaaatta cgagagcaat ttattttgaa agaaaaacta
    29701 gctggtaaat cgataataga acttagcgat attcttgggc tgaaaagccc tgtgacctta
    29761 gaaaaatact acaaatcata atggatataa aacttaaaga ttttgaaggt cctcttgatt
    29821 tactcttgca cttggtatca cagtacaaag ttgacatcta cgaggttcct attgttgagg
    29881 ttattgagca gtaccttaat tatattgaaa ccttacaggt aatgaaactt gaagtggctg
    29941 gtgactacat gctgatggca agtcagctca tgttaatcaa aagtagaagg ctattaccaa
    30001 aggtggtaga acacatcgaa gaagaagatt tagaacaaga tctgttagaa aagatcgaag
    30061 aatatagtcg ctttaaagca gttagtcaag cgttagcgaa gcaacatgac caaagggcaa
    30121 aatggtattc aaaacctaaa caagaattaa tttttgaaga tgctatctta caagaagata
    30181 aaacggtgat ggatctattt ctagcttttt caaacataat ggcagctaaa agagcagttt
    30241 taaaaaccaa ccatacagtc atcgagcgtg atgattataa gattgaagac atgatggctt
    30301 ctatcaaaca gcgactggaa aaagaaaacg ttattagctt atcagcgatt tttgaggaat
    30361 gccaaacatt aaatgaagtt atcagtatat ttttagctag tctagaattg attaagcttc
    30421 acgtggtatt tgtagagcag ctctctaatt ttggggctat catactacga aaggagaaga
    30481 aatgacgtat ttgtcacaga tagaagcgct gctatttgtt gcaggtgaag aagggttaag
    30541 tttgcgacat ctagcgtcta tgttatcatt aacgccaaca gctcttcaac agcaattaga
    30601 aaaactctct caaaagtatg aaaaagatca acattctagt ctttgtttga tagaaacagc
    30661 taatacctac aggctagtaa ctaaagaagg ttttgcagaa ctcttgcgtg cttatgccaa
    30721 aacaccaatg aaccaaagtc tatctcgtgc gagtttagag gtcttgtcta ttgttgcata
    30781 caaacaacct attacacgaa tagaaattga tgacattcga ggggtaaact ccagtggagc
    30841 tttgagtaaa ttattggcat ttgatctcat tagagaagct ggcaaaaaag atgtggttgg
    30901 gcgtcctcat ttgtatgcga caacagatta ttttctggac tatatgggaa ttaatcattt
    30961 agacgaattg attgaagtat ctgccgttga accagcagat gaagagattg ctctttttag
    31021 gacacaagac tgagttttgc ttgtcatcaa atagcaaatg gcgtttacaa aaggatttgt
    31081 tttaaggcgt tttagttgaa aaaaagcctt aaactaggga caacaaaaag ttgctcaaaa
    31141 gaaagaagaa tataatgcga attaacaaat acattgccca tgcgggtatt gccagtcgac
    31201 gtaaggctga agaattgatt aagcaaggct tagtaacctt aaatggtcag gttattacgg
    31261 atttagcaac cactgtaaaa tcgggagacg tggttgaaat agaaggaagt cctatttaca
    31321 acgaggaaaa ggtttactat ttgcttaata aacctcgtgg cgctatctca agtgtgtcag
    31381 atgacaaagg ccgtaaaacg gtactggatt tactgcctca ggttaaagaa cgcatatacc
    31441 ctgttggacg attggattgg gacacatcag gggtccttat tttaacgaat gatggtgatt
    31501 ttacagatac tatgattcat cctagaaatg aaatagacaa ggtttatctg gcgcgtgtca
    31561 aaggaattgc aacgaaagaa aatcttcgtc ccttaactcg aggaattgtt attgatggta
    31621 aaaagaccaa accagctcgc tacaatattg ttcgtgtaga agctgataag aatcgatcga
    31681 tcgtagaatt gactatccat gaagggcgaa accatcaggt taaaaaaatg tttgagtctg
    31741 tggggctttt ggttgataaa ttatctcgta cacgttttgg gaccgtcgac ttaaagggac
    31801 taagaccagg agaagcaaga cgcttaaata aaaaagaaat aagtcaactg cataacttag
    31861 ctaatacgaa aaaatgatga aaaaactatt aatagtatct gttaaagctt atcaaaaata
    31921 catttctcct ttatcgcctc ctagttgtcg ctacaagcca acctgttcag cttatatgtt
    31981 aactgctatt gaaaaacatg gtactaaggg aatactaatg gggatagcaa gaatattaag
    32041 atgtcaccct tttgttgctg gtggtgtcga tcctgttcca gaagatttta gcctaatgag
    32101 aaataaaaac acgtctaaaa acgctgaaaa ggcatgactt tatcaaaaaa tggcttgaca
    32161 ttgatagctg aagtgttata atggaaaaca agcattcgtt ggtttaaatc aaacctgtta
    32221 tgatttaagt tagcgagtca ccatcaacca cattgtttgc tgagcatgac tccgagcagt
    32281 gtggtttttt aaattaaaaa gagtaaatga gattatgacg acaaaagaac ttatcaacaa
    32341 aaacgacaaa gtaaaaaagg ctcgcaatca tattgtcctg tttcaacccc aaattccaca
    32401 aaatactggt aatattgcta gaacatgtgc tgcaactaat gcacctttac acattattaa
    32461 acccatggga tttcctattg atgaccgcaa aatgaagcgt gccggcttgg attactggga
    32521 caagttagaa ctgcattttt atgatcattt agaacagttt atcaatcaat gtcacggaca
    32581 gcttcacctg attagcaagt ttgcagttaa taattattct caagctactt atgctgatgg
    32641 cgattcccat tatttcttgt ttggacgaga agatacaggc ctgccagaag attttatgcg
    32701 agaacatgct gaaaaagcac ttcgtattcc gatgaatgat gagcatgtac ggagtttaaa
    32761 tgtctcaaat acggtttgta tggtgattta tgaagccctt agacaacagg gctttcaagg
    32821 gttagagtta aagcatactt acgaacacga taaactaaaa taatggactt tttaaaagcg
    32881 tcagtttttg gcaacatgcg ttgtcagtag ctgacgcttt taaaaattta gtcaaagcta
    32941 ttgctatcta ggtttaaatc tgctatgcta gaagtgtctt cagggcaggg tgtgattccc
    33001 gaccggcggt gactaggtaa ctagaagtcc gcgagcgcaa gctgatgtgg tgtaactcca
    33061 caaccgacag tatagtctgg atgagagaag accgggcttt ttgtgtaggc ttattttagc
    33121 tacacaataa cgacggaact tctttcaatt tgaaaaaatt ggaggaattt tttaatgtca
    33181 aaaacacata aaatgattat gattggtata ctttctgcga tatcatttct attgatgcta
    33241 gttagctttg ccattattcc aggcgcagct tttttaaaaa ttgaatttag tattattcct
    33301 gtcttatttg gtttaatgat tatggactta aagagtgctt acttgatttt gttattgagg
    33361 tccttattga aactcttttt gaacaatcgt ggggttaatg attttattgg tctcccaatg
    33421 aatattatcg ctatcgcttt atttgtgacg gcttttgcct tagtttggaa ccgtcaaaaa
    33481 acgcttagtc aatatgtatt tgctagctta ctaggcacag ggttattaac gtttggcatg
    33541 gttgttctca attatacttt tgccattcct ttatatgcta tatttgcaaa tattgatatc
    33601 agagcttata ttggtgttac taagtatatg atgactatgg ttattccgtt taatcttgtt
    33661 gaggggttga tatttgcaat taccttttat tttgtgtata ttgcaagtaa accaatttta
    33721 gaaagatact tacactaata tgatgacaaa caaacaaact cactttttaa tagcttcttt
    33781 tgcattgcta atctttgtta taataggcta tacggtcaaa ttttttcctg aacgtttggc
    33841 acttctagat aatactattc aagcagagat tagaggcaac cttcctatag tgctgacgca
    33901 attctttagg ggtgttactg tatttggaaa tgtcatgaca caagttttgt tagttattgt
    33961 gtcggtccta gttttattct ttatgaagtg gaaaattgaa gccttgttta tcttgtcaaa
    34021 tggtgccatt gcagcatttt tgattacgac tctcaagctc ttttaccaaa gaccaagacc
    34081 tgctattgag catttggttt atgctggtgg ctattcgttt cccagtggcc atgcgatggg
    34141 tagcatgctg atttttggta gcctattgat tatttgttat cagaggctac attcaaaact
    34201 attacaattt gtgactagca tgatctttat aatactaatt ttattaattg ggttatctcg
    34261 tatttatcta ggagtacact acccaagtga tattttagca ggctttgtat taggatttgg
    34321 tattttgcac tttatttatc ctttttataa gcaaaaacga tttgaatggc gatttctttt
    34381 gaaacaagac taatttttct tgagaagaca taggcttatt taaatcattt agaataacat
    34441 tggcaagcat cacccccttg attgccagtg ttattttagt tttagttaga tgatgtgagc
    34501 tgatgattag taaatatgtt tagataaaaa acgattaaag tgctatgtaa cgtcagaaaa
    34561 gacgtgattt ttgttgatct gtaatcatta agtaagctga tgtagaagta gtgaattcta
    34621 atgactgagc tatcttttgc atagatgact atcttgctag taatgatttg tgctacaata
    34681 gaaacatgaa aaaaagatat cagacattaa atgagcatta ccgtcaacta tttggcgcaa
    34741 aaatgtttaa agttccgatt gatgccggct ttgattgtcc caatcgtgat ggaacggtag
    34801 ctcatggagg atgtactttt tgcacagtat caggttctgg agatgctatt gtagcaccag
    34861 atgctccaat taaagagcaa ttttataaag aaattgattt tatgcaccgt aaatggcctg
    34921 atgtcaatcg ctatttggtt tattttcaaa actttaccaa tactcatgat acagttgatg
    34981 tgattcgcga ccgttacgaa caagctatta acgaacctgg agttgttggt attaatattg
    35041 gcacacggcc tgactgtcta ccagatgata ccattgctta cttagcagaa ttatcagaac
    35101 gcatgcatgt gacagtggag ctaggcttac agacaactta tgaagaaact tctaggctaa
    35161 ttaacagagc acattcttat gatttgtata aagaaacggt gaggcgacta aggcattacc
    35221 ccaacattaa tattgtctcg cacttgatta atggattgcc aaaagaaact catgacatga
    35281 tgcttgaaaa tgtgagacgc tgtgtaacag acaatgacat tcaaggaatt aaattgcatt
    35341 tgctgcattt gatgaccaat actagaatgc aaagagatta tcatgaagga cgcttaaagt
    35401 tgttgagtca aaaggactat gtttcgatta tttgtgacca gctagagatt atccctaagc
    35461 atattgtgat tcaccgtatt acaggagatg ccccacgcga tatgttgatt gggcccatgt
    35521 ggagtctaaa taagtgggaa gtattgaatg ccattgataa agaaatggaa agacgaggaa
    35581 gctttcaggg ctgtaaagtt gactaatttg tctcattttg acggatttgg ataggagaaa
    35641 agtgatgcta aaacgcccca ttcatctctc gcatgatttt ttagcggagg ttgtggataa
    35701 gtctagtgtg gttgttgatg ccacgatggg taatggaaat gatactgcct ttttggcaca
    35761 attagctaaa aaggtttatg cttttgatgt gcaagaacaa gctattagaa aaacaagtga
    35821 gcggttagcg cagctaggat tatcgaacgc tgagctgatt ttagcgggtc atgaagcggt
    35881 tgatcagtat gtgacagagc cggtgcgcgc agcgattttt aaccttggtt atttgcccag
    35941 cgcagataaa tctattatta ccttaccaaa taccacactc caagctttat caaaactact
    36001 aacgttatta atggtaggtg ggcgtattgc tattatggtc tattatgggc atgatggagg
    36061 cagtttggaa aaagatgctt tattagattt tgtcaagcaa ttagaccaaa gaaaggtttc
    36121 tgcaatgctt tatcagcctc ttaatcaggt gaatacccca ccttttttaa tcatgttgga
    36181 gaaattagca gacgtttagc tcttatccct gtgtcaagtc tactagaagt tctgcgatta
    36241 tttcggtgga aaaagaagaa atcccatagg ctttggctat aaatgtgata taatgaagtc
    36301 actatattat ttttttagga gttacattaa catggaagac cctgtgagtc agcccttagt
    36361 gattcaattt ttattgttag ttgttttaac cttgttaaat gctttttttt cagccagtga
    36421 aatggcctta gtttctctca atcgttctcg ggtggaacaa aaagcagcag acggtgataa
    36481 aaaatacgct cgtttgttgc gggttttaga ggaacctaat cattttttat caacgattca
    36541 agttgggatt acctttatta gtttactatc aggagcaagt ttatcagctt ctttgggtaa
    36601 ggtgatctca ggttggctag gtaattcagc gaccgcaagg acagctggta ctatcatctc
    36661 cttggttttc ttgacttatg tctctattgt tttaggagaa ttgtatccaa aacggattgc
    36721 catgaacctc aaagacaagt tggcgattgt ttcagcccct attatcattg gattagggag
    36781 actggttagt ccctttgtat ggctcttatc agcttctact aatttactga gccgacttac
    36841 ccctatgacc tttgatgatg cagatgagca aatgacacgt gatgaaatcg agtatatgtt
    36901 atcaaaaagt gaggcgaccc ttgatgctga agaaattgag atgttgcaag gagttttctc
    36961 acttgatgaa atgatggcgc gtgaagtcat ggtcccaagg accgatgctt tcatgattga
    37021 cattaacgat gatccgcttg aaaatattca ggaaatctta aaacaaagtt tttcacgcat
    37081 tcctgtttat gatgtggata aagataaaat tatcggtctc atccacacta agcgtctctt
    37141 ggagtcaggt ttccgccagg gatttgatca gattaacatg cgaaaaatgt tacaagaacc
    37201 tctttttgtt cccgaaacca tttttgtaga tgatctctta cgccagctgc gcaataccca
    37261 aaatcagatg gctattttgc tagatgaata tggtggtgtg gcaggacttg tgactttgga
    37321 agacttgctt gaagaaatcg tcggtgaaat cgatgatgaa accgataaag cagaacaatt
    37381 tgttcatgag attggagaca atacctatat tgttgttggt actatgactt taaatgagtt
    37441 taatgactat tttgataccg aactagaatc agatgatgta gataccattg ctggttttta
    37501 tttgacaggt atcggaacca ttccaagcca ggagcaaaaa gaagcctacg aaatagataa
    37561 caaagacaaa catttagttc taatcaacga taaagtcaaa gatggccgta ttacgaaatt
    37621 aaaattaatc ctgtctaata tagaacagat tattgaggaa gactaggctc catgcctagt
    37681 ctttttgatg aaaagtgtta taatgaaaat gtaaaaacgt ttacaagtaa tgaaggagat
    37741 gcaatgactg agaaagatta tggacaagta acagggatgg tgcattcgac agaaagtttt
    37801 ggttctgtag atggtccagg cattcgtttt atcattttct tgcaaggctg taaattacgt
    37861 tgccaatatt gccataatcc ggatacttgg gagatggaaa ccaacaattc aaaaatcaga
    37921 acagtcaatg atgtcttgaa agaagccctt cagtacaaac atttctgggg aaaaaaagga
    37981 ggaattaccg tatctggtgg tgaagctatg cttcaaattg attttatcac tgccttattc
    38041 atcgaagcta aaaaattagg tatccatacc acactagaca cctgtggctt tacctataga
    38101 ccgacacctg aataccatca ggttcttgat aacttattgg cagtgaccga tttgatttta
    38161 ttggacttaa aagaaattga tgaaaaacaa cataaaattg tcacgcgtca gcctaataaa
    38221 aacatattgc aatttgctag gtatttatcc gacaagcaga ttcctgtctg gattcgacat
    38281 gtcttggttc caggcttgac tgatattgat gatcatttga cacgattggg tgagtttgtc
    38341 aaaaccttga aaaatgtcga taaatttgaa gttttgcctt accataccat gggagaattc
    38401 aagtggcgtg agctaggtat tccttatcag ttagaaggtg ttaaaccacc aactaaggaa
    38461 cgcgttcaaa atgctaaaaa tctgatgcag accgaatctt acacagaata tatgaatcgg
    38521 attcatcagt cataagagct gttatagggt tgaggtttcc tcaacttttt aaagtattat
    38581 aagcagatct tttgtaatct tcctttgatt ttagggggta attttgctaa aataaaatga
    38641 ataaacaaaa tgaggtagaa tgattatgtc aaaaatttta gtttttggtc atcaaaatcc
    38701 tgatacggat gctattgcat catcttatgc ttttgattac ttatcccaaa aagcgtttgg
    38761 tttggatact gaagtcgtag ctttaggaac acctaacgaa gaaacagctt ttgcccttga
    38821 ttattttggt gttgaggcac cacgtgtggt agagtctgct aaagcacaag ggtctgaaca
    38881 agtgattttg acagaccaca atgaattcca acaatccatt gcagatatcc gtgaggttga
    38941 ggtatatggt gtggttgatc accaccgtgt ggctaatttt gaaactgcaa atcctttata
    39001 catgcgtgtg gaaccagtag gatcagcctc atctattgtt taccgtatgt ttaaagaaaa
    39061 tggcatcgag gtgccaaaag cgattgcagg gatgctcttg tctggtttga tttcagatac
    39121 cttgttgctg aaatctccaa caactcatgt gtctgatcac cttgtcgctg aagaattggc
    39181 agagctagca gaagtgaact tggaagacta tggaatggct cttttaaaag caggtacaaa
    39241 ccttgcaagc aaatcagaag tagaattgat tggtattgat gccaaaacat ttgaattaaa
    39301 tggcaatgct gtgcgcgtgg cacaagtgaa cactgttgac attgcagaag tcttagaacg
    39361 tcaagaagct attgaagcag ccattaaaga tgccatggct gcagaaggtt attcagattt
    39421 tgttttgatg atcacggata ttgttaactc aaactctgaa attctagcta ttggagcaaa
    39481 catggacaag gttgaagcag cctttaactt cacccttgac aacaatcatg ccttcttagc
    39541 aggtgccgtt tctcgtaaaa aacaagtggt tccacaattg acagaaagtt ttggagctta
    39601 atgaaaagac gctttaaaag gacttgattt ttttgaccaa gtccttttgt cactcttcta
    39661 tgttataata gaaggtatga taacagtatt tcattcagat aaactcacca gacaaccttt
    39721 ctttcaagac cttatcaatt atctcgacca gcatgatcac gttatattac gagagatcaa
    39781 aaaagccttt cctaatgtga caggtattga caaggccatc gaaagctatg ttcaagctgg
    39841 ctatattcgc cgtgaaaata agcgttatgg aatcaatctt cctttggtga gttctgatca
    39901 gcagctggcc ttagacacta tgctttttgt ggacacctgt tcagctatgt atgaaaatat
    39961 tttagcggtt gtttttgaga ctcagctaac aaaccaaacc aatcacgtga tgatcaagga
    40021 aaagaccaac atcacgagag acgatttgac cctggctaat tatttttacc gtctcaaaag
    40081 aggtgagaag ccatcagctg agcagctgga cttgtatgac ctcttggggg atgtgaatca
    40141 ggaatatgcc cttaaatata tgacaacttt tttgcttaaa ttcacgcgca aagaccttgt
    40201 gatgcaaaaa cgtcctgata tatttgtgga agctctggtt acacttggtt acctaaaaca
    40261 agtggagcct acaacatacc agctgctaat gaccttggat aaagaaagtt taacctttat
    40321 agcaccatag aaaaaagcta ggaacaagga ctgttcttag ctttttgagt gcttttattt
    40381 tgtttttgcc attaagtaaa gaaaataagg ggctccgata agcgacacta aaatccctgt
    40441 ctggatatta gtgccaacca gatacactcg cccgactgta tctgcaacaa gaagtaggat
    40501 cattccgatt aaaatactaa tgggtattgt tattttatga ttactcccta ccaagtatgt
    40561 actaaagtgt ccagctatta agccgataaa actaatatta ccaaccaaaa gaacactcaa
    40621 agcacctaag ccagctgcta agacaagtat caaacgacgc ttacggttta aagggagtcc
    40681 tagcccaata gcagtattat cagctaatcc cataatatta aggaaatggg cttgactata
    40741 agtcaatagc caaaaacaca ataataaagg agcgatgaca ctcagagtag gccagtcgtc
    40801 tcctattaat tggccgctaa gccaattgat gaccaaatcg actttgtagc gattaatatg
    40861 accaacaaga gcaaccatta gacttgaaag catggtagta acagctacac cagtaagaat
    40921 taaccgtgta ggatcaattt gtccttgttt tgtcaaagat agccaataaa cgctaaaggt
    40981 tgttacaaca gcaccaagca tggcaaataa aggcaagtaa tggctgatat gagcttgctc
    41041 aaagaaagaa aaggagacca caatagccaa agagatacct gctcctatag taatacctaa
    41101 tacaccagag tcagctaagg gatttcgtgt taaacgttgt aataatagcc ctgataaagc
    41161 aagagaaccg cctcctagac aagcagctag aattcttggc agacgaatgt taataacgat
    41221 gaaagatatg gcatggctag attttcccaa aaacacatgg ataaggtcca aaaaagatag
    41281 atgagattct ccaagggaca aagctaatcc cataagactt acaagtagca ggatcaggct
    41341 agtatataac cccaaaaggt gtcggtgaga tttcaatagc ggcctcctct tcttattaac
    41401 cataaaaagc atggaaaacc aattaagcta accaatgccc caagaggggt ttcataaggc
    41461 ggattgagat tacgacaagc aatatcaacc cataccatga aactagcgcc agaaactgca
    41521 caaagcggca aaaggtatcg gtaatgatga ggtgtaaagt gtttcataag atgaggaatg
    41581 actaagccga taaaagaaat gcttccagca atggctacag ctgctgatga taaaataaga
    41641 accaaaatca taaagactgc actgatcaag ttggtctttt gcccaagagc ttttgcctga
    41701 gactcactta gactaaggac ggtcaggtga taagataata gctgggctaa acaaagacta
    41761 agaataataa gaggagcaat atagccgatc atttgccaat tgaccccgac aagacctcct
    41821 gcttgccagc cgataacagc atttgccaga tgatagtaat tggtaatacc ttgacctaat
    41881 gctgatagca gtatggaaac catagctcct gctaagacaa ggcggagctg atggtagcct
    41941 ttgccagatt gataagaaag gccaaagact aaggttgctg ccaggctaga ccctaacaac
    42001 gaaagcagaa taatgaggga atagtgcaag tgaggtacaa acgcataagc cagtaccaag
    42061 gctagtcctg ctccagcatt aatgcctaaa aggccaggct cagcgatagg attacgtgtg
    42121 attgcttgca tgatagttcc agaaacagct aacgcagagc cagttaaggt agcaccaaaa
    42181 agtctcggta atcgtatagc gagtaaaaca ttggctttat ggccgttttg ctttccaaat
    42241 aaaatagagt taagatcctg atgtgataaa gcaacggctc caaagcgcag atgacagtaa
    42301 atagcaacac ataagctaaa actaagtagc acaaagacaa gccaaaagat atgtgactta
    42361 gtgattatct gcgttttttt taagcaaaaa cgctcgagac acaaaaaggg atgcttagtt
    42421 ttcacttgat aagattgcct ctcgtaaagt ctctaattga tattctaaag aaagaggatc
    42481 agtaaagtaa aaaacattgg cattgacttt gatcacatgg tgttttttca cagctggaat
    42541 ggattgccat agcttacttt catatagagc agaaccagtc ttatcatctt ctgcagcaac
    42601 gacgacataa tcaccaatat agtctggcaa gacttcctgc gacaatgaca ggtaaccttg
    42661 tttaaaaact tcagtcttca ctttttcagg agcatcataa tggaaagctt gatggataac
    42721 ctctccgcca cgtccccagt ctttaccaaa aaggtaaacg tctttttcat aaagccccat
    42781 aatagtaaag gttgctttgt cacctgtaac ggctttgact tctttttcat aagctgctgt
    42841 tttagttttc caatctttta accatttctt ggctttgcct tctttgttaa agatgcggcc
    42901 gaagtcagat aacacttgta aataatcacg tttgcggtat tcaatcgaaa taacaggcgc
    42961 aatttctgcc aattgtttaa tattttcttc tgtagagcca acaacaatga ggtcaggctt
    43021 aagcgttgta atggcttcta aatcagttgc agcaacttgc tttgcttttt ttacagtctt
    43081 tgctaagatg gggtttttct tgtcataaga agtaaccccg actagattca tatctaattt
    43141 tttgaggtaa ccagtgtaag ttgaagccag actgactact cgcttaggct gtttaggaat
    43201 atctccgtaa taagtcacac cagcaatttg aggcataggc gataaacttt ttgaggcggt
    43261 gttagaatga ttagttgctt gattaccaca ggcgattaag gtaatagttg tcaaacagag
    43321 tgttaatagt agagttaatt ttttcatgta tgctaattct cctagaataa agttattgaa
    43381 gctgataagt cagcactata ggacagttgt ggataggatc atcaaccagt accggtttga
    43441 tttggaagat gtcttgaatg ataggtgaag tcataacatc tgctatagta ccagtataat
    43501 ggatcttgcc atgtttcata gcaatcagat gatccgaata gcgggctgaa agattgaggt
    43561 cgtgaagaac cataacaata gtcttgccgg catccttatt tagggattta acgagctcta
    43621 ggatttctag ttgatgattt aaatctaaat aagtagttgg ctcatctaaa aaaatagtat
    43681 cagtgccttg ggctaatgcc attgcaagcc aaaccctttg acgttgcccc ccagaaagag
    43741 catcaacagg ttgatcagca taggccatga cattggtggc ttgcatagcc caatgaatgg
    43801 cttttttgtc agctggacta agatttccaa agtaagattg atggggaaaa cgtccgtaag
    43861 aaaccaattc gtaaacagtt atgccgttag ttgcttcttg aacttgaggt aaaagagcta
    43921 gttttttagc aacctcttta gtttctaatg tggcaatgtt ttgcccattt aaatatacaa
    43981 ctccctgttt tggtggtaat aatcttgtca gtgcttttaa taaactagac ttcccacagc
    44041 cattggcgcc aataatggtc gtaattttac cttcaggaat ataaaacgat aatttatcga
    44101 tgatggtacg ctgttcatag gcaattgtga ggtcttcagc actaattgtt gtcatatggt
    44161 tgtctccttt ctaaaaagag tcaaagggtt gatgaaagtg cacattaaag tattcatctg
    44221 cccattttaa aatagttgag aagtagctta ttttctcaaa atcttcacta ttatagcata
    44281 gaaaaaaggc ttaacaaagc cttttttgat gtgattttta ggatgtatta aaaaagaaat
    44341 attagtttag aatgattaaa aacaatagcg ataatcattc taaactaacg tcaacatact
    44401 ctagaggtag tgtctggcga ttgctaaatc accagcataa gctacttgtt gtcctttaac
    44461 aatttgataa gcatctgcgc ctttcccagc gataatcaca gcatcttttg ctccttggca
    44521 aagagacatg gctttttgaa tagcttgctc acggtcacta ataatctcta ctgggcgtgc
    44581 gatatggctg gcaatttctt ttgaaatatc ttctggatcc tcgaaattag ggtcatcagc
    44641 agtgagaatg acggtgaggt ttggatgctg atgaatcacc ctaccaaaat cagctcgtct
    44701 gctctcgcct ttatttcctg gtgcacctaa aatcaacatt aatttgcctg tctgatgttc
    44761 ttccacaacg ctgagtaatt tttctaagct gtcaccatta tgggcataat caacaaaaac
    44821 tttagcatga tttgtcatag tgaggacttc catacggcct gggacgcgag tcttagcgat
    44881 acctttttga atatcagcta ggctagcacc caaacgaagg caggcaagtc cagctgccat
    44941 agcattttct tggttaaaat ggccaattaa ttgaatgtca taatggccag ctaattgtcc
    45001 tttagcttca aaggagaagg cttggctagt ggtgatctgg ttgtcagaca agggaccata
    45061 aaacacatgc tcttgatccg ctacctgatc cgctagaaat gaaaaatgat ccatcccgct
    45121 attgatcaca acagctctgc tattctccat taaaagacgc ttatgataga aataatcttc
    45181 aaacgttgga tgctcgattg ggccaatatg atcaggacta atatttagga aaaccccaac
    45241 atcgaacgta agcccataga cacggtcaac gaggtaagcc tgactagaaa cttccataat
    45301 caagtgggtc atgccattag tcacacactc agccatcata gcaaaaagat ccaagctctc
    45361 aggagttgtg agttgtgatt tgaaaaaggt cttgccatct aaggtggtat tcatggtaga
    45421 aaacatggca ggtttatagg attctttcaa catgtggtag gcaaaataag cagccgtcgt
    45481 ctttccctta gtacctgtaa aagctagtag tttgagcttt tcttgagggt tgccatagaa
    45541 aaccatggca attagactca tagctttttt gatatcagtg accaaaacaa cgggaatacc
    45601 aagctcgtaa tctacctctg aaatatagag ttgtaatccg ttagtgattg cttctttaag
    45661 gtagtcagct ttgaaagttg cccctttagc aaaaaaaagg gtcttgccat ctacttgacg
    45721 gctatcgtaa ctcaagcgct caaaactaaa gccttgataa tggtagtggt atccgtcggc
    45781 atcaagaacc tcacgaaaat tatggtcttt ttttaaaatg tctaataatt gttcaatggt
    45841 tatcataccc ttattttatc cttaaagttc ttgttttaca agtcacaagc aaaagtttat
    45901 aataaagcta atagaaacta ggaaagaata gatatgtcga cagaaaaaaa acaactgaca
    45961 caagaagaat tgatggttca gggagcagcc tggtccacag ctggcaattt cattagccga
    46021 cttttgggag tgttatacat tattccctgg tatatttgga tggggcaata cgcgattcaa
    46081 gccaacgctc ttttcaatat ggggtataat gtttatgctt attttttatt gatttcaacc
    46141 acaggactca atgttgcgat tgctaagcag gttgccaagt acaattccat gggacaaact
    46201 gagcatagct atcaattaat ccgaagtacc ctaaaactca tgcttggtct gggccttatt
    46261 ttttcagcca ttatgtatct gggctctcct ttatttgcga gcttatctgg tggtgatgac
    46321 acacttgttc ccattatgca cagcctttct ttagcagttt ttatttttcc tgtaatgagt
    46381 gtcattcgag gtatctttca aggccacaat aatatcaagc cttatgcgat tagtcagatt
    46441 gctgagcaac tcattcgggt tatctggatg cttttgacca ccttccttat catgaaatta
    46501 ggttctggtg attacgcttc agcagtaact cagtcaactt ttgcagcctt tatcgggatg
    46561 gtggctagta tgggtgttct ggggtactat ctttggaaac agggacttct agcagctatt
    46621 tttagtaagc cagatcatac tgtttccatt gatatcaagg gcttgttgct tgaaacctta
    46681 aaagaatcca tcccctttat cgtaacagga agtgccattc aagcctttca attgattgat
    46741 caatggactt ttgtcaatac tatgactctt tttacagatt atagccgttc tcaattgctt
    46801 gttttatttg gttactttaa cgcaaaccca gccaaaatta caatggtttt aattgcagta
    46861 gcagcatcta ttggtggtgt aggcattgcc ttattaactg aaaattatgt caaaaaagac
    46921 atgaaagcag ctgctcgctt aatcattaac aacattgaaa tgttagtaat gtttttgtta
    46981 cccgctctta ctggggcaat tattttagca agacctctat attctgtttt ttacggagct
    47041 agcgaggatc gtgccattca cctctttgtg gcggtcctct tccaaacctt gttgatggcg
    47101 ctttacaccc ttttttcacc gatgcttcaa gctctttttg aaaatcgaaa agcgatttac
    47161 tactttgcct atggtatctt gattaagtta gttttacaga taccgcttat ttatttgcta
    47221 catgcttatg gtcctttact agcgacgacg attgctttag tggtgccgat ttatttgatg
    47281 tatcgacgtc tatatcaggt tactcatttt aaccgcaaac tgttgcaaaa acgtttatta
    47341 ttaaccttga ttgaaacctt attaatggga ctggtcgtgt ttgtggccaa ctggctattg
    47401 ggctatgtct ttaaaccgac aggccgcttg accagccttc tttacctcct cattattggt
    47461 ggcttgggaa tgacggttta cacggcactc accttgctga cgcatcaact ggataaatta
    47521 attggtagca aagctagtcg ccttcgtcag aaattaggct ggcattaata taatgacacc
    47581 aagaacgatt tcgtgagtgc ttatcgaaat cgtttttttc ttaatgatct cgcttagtaa
    47641 aggtagccga taaaaaatca ggatttccca agcaaactac tgatattttt ggtataatga
    47701 taaaagtgta aaaaaataga aggaagtata tcaacatggg aaaatgtcaa gttatttcgc
    47761 atccgcttat ccaacacaaa ctttcaatct tacgtcgtca aaccacatca actaaagatt
    47821 ttcgtgagtt ggttaatgaa attgcgatgc tgatgggata tgaagtgtca cgcgaccttc
    47881 ccttagaaga tgtcgacatc caaacgccag tatcaaaaac ggtccaaaaa cagttagctg
    47941 gtaaaaaatt agctattgtt ccgattcttc gtgctggtat tgggatggtt gatggcttgt
    48001 taagtttggt accagctgca aaagttggcc atatcggcat gtaccgtaat gaagaaactc
    48061 ttgaacctgt tgaatacctc gttaaactac cagaagatat taaccaacga caaattttcc
    48121 ttgttgatcc aatgttggca acaggtggtt cggctatctt agcagtggat tcgttgaaaa
    48181 aacgtggtgc agcaaatatt aagtttgtct gtttagttgc agcaccagaa ggggtgaaaa
    48241 aacttcaaga agctcatcca gatattgata tcttcacagc agcacttgat gatcatttaa
    48301 atgaacatgg ctatatcgtg ccaggattgg gagatgctgg tgatcgttta tttgggacaa
    48361 aataaaagag acaaccatca aatagtagcc tttttaggcc aatcgttctt gattttgtta
    48421 aaaaaatcag agagatttct cttctttaaa ggaaaagcaa gtttatgatt tgacctttat
    48481 tgaccaaaat attataataa agacaatcag taaaaaagag atgacctagt gggttgccgt
    48541 ctctcaaaat gacgctacta aaaacccttg tagattggag aatgtattat gattcctgtt
    48601 gttattgaac aaactagccg tggagaacgt tcttatgaca tttattcacg tttattaaaa
    48661 gaccgtatta tcatgttaac agggcctgtc gaagacaaca tggccaactc tgtcatagcg
    48721 cagctcttat tccttgacgc gcaagacaac acgaaagata tctacctata tgtcaacaca
    48781 ccaggtggct ctgtctcagc aggcttggct attgtcgata ccatgaactt tatcaaggcc
    48841 gatgtgcaaa ctattgtcat gggtatggca gcttctatgg ggacagtcat tgcttcatca
    48901 ggaacaaaag gcaaacgctt tatgttacca aatgcagagt atatgatcca ccagccaatg
    48961 ggtggcacag gcggtggcac gcaacaaacg gatatggcta tcgcagcaga acatctctta
    49021 aaaacacgtc accgtttaga aaaaatctta gcacaaaatg ctgggaaaac gattaaacaa
    49081 attcataaag atgctgagcg tgattattgg atgagtgctg aagaaacctt gacttacggt
    49141 ttcattgatg aaatcatgga aaacaacgaa ctaaaataaa gaggatatct gcaaaagtat
    49201 tctagaaatt tctaggatgc tttttctctt agaaaggaaa ataatgatac agcctactgc
    49261 attaaaacag cttcaagagt taaaacgtca gttcagatcc ctttgcaacc aattatgaga
    49321 tagtttgagg atgatcctta gcagctgtgt gcaagaagtt atgacgattt cataaaggat
    49381 gacctagcca gtctttcttc gtctctttta cttaaagtgt ggtataatca gattatgcta
    49441 gccaaaatta aaaaaggcat tgccattaaa ggtgttgtag atcggcttat cctagatctc
    49501 ataacaatgc tgtagtaccg cccttacctt gctactaaag gcgctgttga agcacaaatt
    49561 gacttgcaaa ggatcttgac tgataggtat atgtctggtg gaatcgccaa agcagcaaaa
    49621 agaccaagac ccttatttgg tctctaccaa aatataatag aaaaggattt agaatgtttc
    49681 aaaaacaaga acgtattgga ctcgtcgttt acctgtatta taaccgagat gccagaaagt
    49741 tatcaaaatt tggtgattta tattaccatt ccaagcgctc tcgttatctc attgtttata
    49801 ttaataaaaa tgatttagac acaaaattag aagaaatgag acgtttgaaa tgtgttaaag
    49861 acattagacc atcagctttt gatgatattg accgccaatt tgtaggtaac cttcatcgcg
    49921 acgaaacaaa caaccatcaa aagggttaac aaccccctag ttactgatta gcgttttaga
    49981 tttagttagc ttattaaagt aagaaggttt tgacagatgc tcacactact tcaagacaat
    50041 gagacaacta gagcattagt gaataactga gttgacggtc acttctttaa taagaacgga
    50101 taggattggt cctaaaatag agcactgcaa agatatgcca gtgcttttta gctcattttt
    50161 tgataaaata gaactactaa aaagtgaagg aaataagatg atcacaggaa aactcataac
    50221 cgttgaaggt ccagatggcg cagggaaaac gactgtttta gagcaactca ttccactgtt
    50281 aaaacaaaaa gtggctcagg agatcttgac aacaagagag cctggaggtg ttgccatttc
    50341 agaatacatt agagaactta ttttagacat caaccatact actatggacc ctaaaacaga
    50401 actcttactt tatattgcgg ctcgaaggca gcatttggtg gaaaaggtac taccagctct
    50461 tgaagcaggc cagttggttt ttattgatcg tttcatcgat agttcagtag cttatcaagg
    50521 tgcaggacgc ggtttgatca aagcagacat tcagtggctc aacgagttcg caacagatgg
    50581 tttagagcct gacttgaccc tttattttga tgttccttct gaaattggtt tagcacggat
    50641 taacgcgaac caacaaagag aagtcaatcg tttagatttg gaaacaatag agatacatca
    50701 acgagtacga aaaggatacc tagccttagc taaggagcat ccaaaacgta ttgtcacaat
    50761 tgatgcgact aaaccattga aagaagttgt ctctgtcgct ttggaacatg tcttagcctt
    50821 gcttttagcc taggataagg gaggggagga atggatttag cgcaaaaagc tcctaacgtt
    50881 tatcaagctt ttcagacgat tttaaagaaa gaccgtctga atcatgctta tcttttttcg
    50941 ggtgattttg ctaatgaaga aatggctctt tttttagcta aggtcatctt ttgtgagcag
    51001 aaaaaggatc agacgccctg cgggcattgt cgctcttgtc aattgattga acaaggagat
    51061 tttgccgatg tgacggtatt ggaaccaaca gggcaagtga ttaaaacgga tgtggtcaaa
    51121 gaaatgatgg ccaacttttc tcagacagga tatgaaaaca aacgacaagt ttttattatc
    51181 aaagattgtg acaaaatgca tgtcaatgcc gctaatagct tgctaaagta tattgaggag
    51241 cctcagggag aagcttacat atttttattg accaatgatg ataacaaagt gcttccgacc
    51301 attaaaagtc ggacacaggt ttttcagttt cctaaaaacg aagcctatct ttaccaattg
    51361 gcacaagaaa agggattatt aaaccatcag gctaagctag tagccaaact tgccacaaat
    51421 accagtcatc tagaacgtct gttgcaaacg agcaagcttt tagaactgat aactcaagca
    51481 gagcgttttg tatctatttg gctgaaagat cagttgcagg catatttagc gttgaaccgt
    51541 ctggtacagt tagcaactga aaaagaagaa caagatttag ttttgaccct tttgaccttg
    51601 ctcttggcaa gagagcgtgc gcaaacgcct ttgacacaat tggaggctgt ctatcaggct
    51661 aggctcatgt ggcagagcaa tgttaatttt caaaacacat tagaatatat ggtgatgtca
    51721 gaataagtgc aattggaatt cgttatgggg aaacgaagct tgtcaaaaga cttgcccgta
    51781 ctcccttttc agcagaagag cacatggatt tttacaactg ttaaaagatt tagcggccga
    51841 ttttaggtca agaagtgagc tgccccagat taacagccaa gaagatttca ttttctatgg
    51901 aggctatgga ccgtgtggca ggccactgtg ctgcattcgt ttttgggaaa atttctgaca
    51961 gtatctatta aaatactaaa aaatcaaggg ttatcattga ctttgggtaa agcaacaagc
    52021 ccttatggcc gtttgctttg ctgtctctat gatgaaggtg gcttttacca ataaagcaag
    52081 aaaaaattcc ctgaatatgg taccatagta gagacaagtg atggccctgc aacagtagca
    52141 gccgttgata tatttacaga tcccattaag gtgcggttaa aagaccagtt gaccttagtc
    52201 acttatgctt tagaggaggt taaagtaggt gaataaaaaa gaattattcg atgcttttga
    52261 tggattttcc caaaatttaa tggttacttt agctgaaatt gaagccatga aaaaacaagt
    52321 ccaatcacta gtagaagaaa acacgattct tcgcctagaa aatacaaaac tacgggaacg
    52381 tttgagtcac ctagaacatg aaacggtagc taaaaatcct tccaaacaaa ggaaggatca
    52441 cttagaggga atttatgatg aagggtttca tatctgcaat tttttctatg gacaacgtcg
    52501 tgaaaatgat gaagaatgta tgttttgcag agaattatta gatagaaagt aagggcatgc
    52561 aggttcaaaa aagttttaaa gacaaaaaaa caagcggaac cttgtatttg gttccaaccc
    52621 caattggcaa tctgcaggac atgaccttta gagcagtggc aacgttaaaa gaggttgatt
    52681 ttatttgtgc agaagacaca agaaacacag gtcttttgct aaaacacttt gacattgcta
    52741 ccaagcaaat cagctttcat gagcacaacg cctatgagaa gattcctgat ttaattgatt
    52801 tattaatatc aggacggtca ctagctcagg tgtctgatgc aggaatgcca tcgatttctg
    52861 atcctgggca tgatttggtc aaggctgcta ttgatagtga tatcgcagtc gttgctttac
    52921 caggtgcctc agctgggatt acagctttga ttgctagtgg actggctcct caaccccatg
    52981 ttttttatgg gtttttaccc agaaaagcag ggcaacaaaa ggctttcttt gaggacaaac
    53041 accattaccc agaaacacaa atgttttatg aatcgcccta tcgcatcaaa gataccctga
    53101 ccaatatgtt ggcttgttat ggtgatcgtc aagtggtgct tgtcagagaa ttaaccaagc
    53161 tttttgagga gtaccaacgg gggtcgattt cagaaatctt gtcctacctt gaggaaacac
    53221 ccctcaaagg ggagtgtctg ctgattgtgg ctggagccca agtagacagt gaggttgagc
    53281 tgacagctga tgttgactta gtgtctctag tgcaaaaaga aattcaagca ggagccaagc
    53341 caaatcaggc tattaaaaca attgctaaag cttaccaggt aaatcgccaa gagctttacc
    53401 aacaatttca tgacttataa gtcatcgtag gttaggagaa aaacaaatga cagaagatca
    53461 agcaactatc cttattttag gagtatacgg aatcatattg gctgtattct tattatcagc
    53521 tttattatgc cttatcgcta actggcgact ctttaaaaga gcaggttaca aaggatggta
    53581 tagtctcatt cctttctatg caggttttgt caaacacaaa atcacttttg gagaagataa
    53641 taaatggttt tactttattg gttgggtcat tgctatttat tattactatg tgcacttttg
    53701 ctatgtccgt gcttttggtg gatcaaaggg tctagcagta tttggcttgt ttttcccagg
    53761 cattgcttct ttgatagtgg cttttggaaa aacttatacc acacaagaat gccaccctgt
    53821 gccacatctt ttaaactaag agaaaaaaga ccatcaattg gatttattga tggtctttta
    53881 ggtgtttaaa tgattttagt cccgtgaaca atggccgttc cagtgagttg actcaagctt
    53941 tggcaattct cagcagtgac gcctccccca atcatgattt caatgcgttt attggcatag
    54001 gtcacaagac ttttaagctg ttccacattg tctgtaatag gagtagcttc aggtgagcca
    54061 tgagttagca cacgcacaaa gccataatca atcagttggt caagcgcttg gtgctggtga
    54121 tcagtcggaa tccggtcaaa agccatgtgg aaaaccagtg gcaagccttg tgtagcaggc
    54181 agtaattgct cgatagcgtc ggtatccagt tgattttctg aggtcagcaa gcctaagact
    54241 agcgcatctg agccagcttc aacagctttt aagatatctt cttccatagc ttttagttcg
    54301 aggtcgttat agacaaaatc gccaccgcga ggtcgaatca tggtcgctac gctaatcttt
    54361 ttatcgtgta agagctgaca ggcttctttg atgacgccat aagatggcgt ggtccctccg
    54421 acagctaggt tatcacagag ttccacacga ctgacttgac cagcatcaag ggttggcagt
    54481 agtgtgaggt tctcagcaca aaattcttta atcattttta gttctccttt atcactaata
    54541 ttatagcacg aatgaagcta gtcatcagga aacatttttg tcaaatcatg gacattatgc
    54601 tatactcact agtattaaaa gtaggccaaa acagcagcaa atactttgaa aaaagattgc
    54661 tatgttctac ggcaatatca gtctttacca acgaactctc aagaagaaaa gaaagacaaa
    54721 gcttagcatt ttaaaaaagc cccccttttt ttgtgaaatg atgaccaaaa acaggcgcct
    54781 cacgcctaaa acagttaccc taaaggagaa caaatgatta cgatttacca atacccaaaa
    54841 tgtagcacct gccaaagagc catggctgaa ttaaagcaac tggttagtga ctttgaagcc
    54901 attgacatca aagctaatcc acccaaagct caggacctca aacattggat ggaaacatct
    54961 ggttatacca tcaaaaattt ctttaatacc agtggtaaca gctaccgcga attgggactt
    55021 aaagataaaa tagaccagct tagccttgat aaagctgctg aacttttagc cacagatggt
    55081 atgttgatta aacggcctat tctgattaag gacggtaacg tcttacaagt tggctatcgc
    55141 aagccatatc aagagttgga tttaggataa tgcgacaaag tctcaaaaaa ggacaaggca
    55201 acattataac gccttgtcct tttttgggat ctgtttttag acattagaga tcaatatcta
    55261 gtaaaattgg cgtatggtct tggcgttccc cagaagaaat catttcagaa cgcttaacct
    55321 tatccacgat tcggtttgaa gctagccaat agtcaattct ccaaccggta ttattgattt
    55381 tgctggtttt actacgttgt gcccaccagg tataaacatg aggaatatcg ccgtgcaagt
    55441 gacgaaaagt atctgtaaag cctcttgcta ataggttagt aaagccttga cgttcttcgt
    55501 cggtaaaacc aggagaacgt cgattgctgc ttggatttgc caaatcaatc tctttatggg
    55561 caacgttgta atctcccgta gccaaaacag gcttttgagc atccagttca gtcaaataat
    55621 cagcgtattt gtggtcccaa atttgacggt catctaaccg tcttagacca tcaccagcat
    55681 taggcgtata aacctgtgtg acgaaaaagt cttcaaattc tagggtaatg atacggccct
    55741 cagcatccat tgttgttggg gcaccaattt ctggaaaagt aatgacaggg tttaacgtgt
    55801 ttttgtacag gaacatggtg ccagcatagc ctttcctagc aggctcaact gaagaacgcc
    55861 acacatgcaa atagtttgga aagtaactaa gtagggtctc aatatgtttt ttggtaggcc
    55921 ccttagcaga taatttagtt tcttggatgg caataatatc cgcatcttga gcgactaaag
    55981 tgtctaaaac agcgcgtgat aaaagcgcac gaggggactc tcctgtgaga gcagcattta
    56041 aggaatcaat gttccatgaa atcagtttca tttggtttct ctttctaaca gatagtcttg
    56101 ttgttattgt aacaaaaaac gaaacgataa gcgactttgg gcacctagcc cccataaaac
    56161 agtctagcca aatgaagcat agcagcgtaa gaacaagaca aggaggtgat cagtgagcat
    56221 atcgtttgac agttaagagt aggattacta taatagaaac ataaatgtaa gcgctaacaa
    56281 aataaaaaat ataggagaat gtcaatggca caaaaaacag tcatcaccga agaaacaacc
    56341 gattttgtta tggatttcaa aacaagtagc gctgagggaa atgttgattt tattaacgtt
    56401 tttgaccttg agaaaatggc acaacaggtc atccctaaag gagcctttgg ttatattgct
    56461 agtggtgctg gtgatacctt taccctccat gaaaatattc gctcatttaa tcataaatta
    56521 attgtccctc actccctcaa aggggttgaa aatccgtcaa cagagattac gtttgatggt
    56581 gatcacttaa cctctcctct tattttagca cctgtagcgg ctcataagct ggccaatgaa
    56641 caaggagaag tagcgtctgc caaaggattg aaggaatttg gaagtatcta caccactagt
    56701 tcctactcaa caactgacct gccagaaatc agtgctgcct taggtgggac accccattgg
    56761 tttcaatttt attgcagtaa agacgatggc attaatcgta atattatgga tcgtgtcaaa
    56821 gcacaaggtt gcaaagctat cgttttgaca gcagatgcaa cagttggagg aaaccgcgaa
    56881 gttgataggc gaaacggctt tgtttttccg gtcggtatgc caattgttca agaatattta
    56941 cctgatggtg caggtaaaac aatggattat gtctataaat cagctaaaca agcgcttacc
    57001 tcaaaagata ttgaatatat cgcaacctac tcaggcctgc ctgtttatgt caaaggacca
    57061 cagtgtgctg aggatacctt gcgggcattg gacgcaggag catcaggtat ctgggtaacc
    57121 aatcatggtg gccgtcaatt agatggtggt ccagctgctt ttgattcctt acaagaagtc
    57181 gctgaagcag ttgatcacaa agttcctatt gtctttgact caggtataag acggggtcaa
    57241 catattttca aagctcttgc ttcaggtgct gaccttgttg ccttggggcg tccagcgatt
    57301 tacggcttgg ctatgggagg aagcattggt actcgtcagg tctttgaaaa gttaaatgac
    57361 gagttaaaaa tggttatgca actggctggg acacaaacta tccaagatgt taaagccttt
    57421 aaccttcgcc acaatccgta tgattcatcg attccatttg atcaaaatgc actacgctta
    57481 gattagaaca gacaaaaacc tctgctatca tgtcacaaat agtagaggtt ttacactgca
    57541 aaaagaggta tgaaaatcca ataacatagt tacaaggctt tcgtttaata ccccccacta
    57601 atagattaaa atgaaagact tcttacatta atagaagttt ctgttgcttt tctcagaaaa
    57661 atgtggttaa ataatcaaat attattaacg aaaaatgagt taatgattaa cattaaactg
    57721 gttttagtta aaacatttag gagggtatca ggtggagaaa aagcaacgtt tttcccttag
    57781 aaaatacaaa tcaggaacgt tttcggtctt aataggaagc gttttcttga tgatgacaac
    57841 aacagtagca gcagatgagc taaccacaac gagtgaacca acaatcacga atcacgctca
    57901 acaacaagcg caacatctca ccaatacaga gttgagctca gctgaatcac aatccccaga
    57961 cacatcacaa atcactccca agataaatcg tgaaaaagag caaccacaag gtctagtctc
    58021 tgagccaaca acaactgagc tagctgacac agatgcagca ccaatggcta atacaggtcc
    58081 tgatgcgact caaaaaagcg cttctttacc gccagtcaat acagatgttc acgattgggt
    58141 aaaaaccaaa ggagcttggg acaaggggta caaaggacaa ggtaaggttg tcgcagttat
    58201 tgacacaggg atcgatccgg cccatcaaag catgcgcatc agtgatgtat caactgctaa
    58261 agtaaaatca aaagaagaca tgctagcacg ccaaaaagcc gccggtatta attatgggag
    58321 ttggataaat gataaagttg tttttgcaca taattatgtg gaaaatagcg ataatatcaa
    58381 agaaaatcaa ttcgaggatt ttgatgagga ctgggaaaac tttgagtttg atgcagagcc
    58441 aaaagccatc aaaaaacaca agatctatcg tccccaatca acccaggcac cgaaagaaac
    58501 tgttatcaaa acagaagaaa cagatggttc acatgatatt gactggacac aaacagacga
    58561 tgacaccaaa tacgagtcac acggtatgca tgtgacaggt attgtagccg gtaatagtaa
    58621 agaagccgct gctactggag aacgcttttt aggaattgca ccagagaccc aagtcatgtt
    58681 catgcgtgtt tttgccaacg acgtcatggg atcagctgaa tcactcttta tcaaagctat
    58741 cgaagatgcc gtggccttag gagcagatgt gatcaacctg agtcttggga ccgctaatgg
    58801 tgcacagctt agtggcagca agcctctaat ggaagcaatt gaaaaagcta aaaaagccgg
    58861 tgtatcagtt gttgtagcag caggaaatga gcgcgtctat ggatctgacc atgatgatcc
    58921 attggcaaca aatccagact atggtttggt cggttctccc tcaacaggtc gaacaccaac
    58981 atcagtggca gctataaaca gtaagtgggt gattcaacgt ctaatgacgg tcaaagaatt
    59041 agaaaaccgt gccgatttaa accatggtaa agccatctat tcagagtctg tcgactttaa
    59101 agacataaaa gatagcctag gttatgataa atcgcatcaa tttgcttatg tcaaagagtc
    59161 aactgatgcg ggttataacg cacaagacgt taaaggtaaa attgctttaa ttgaacgtga
    59221 tcccaataaa acctatgacg aaatgattgc tttggctaag aaacatggag ccctgggagt
    59281 acttattttt aataacaagc ctggtcaatc aaaccgctca atgcgcctaa cagctaatgg
    59341 gatggggata ccatctgctt tcatatcgca cgaatttggt aaggccatgt cccaattaaa
    59401 tggcaatggt acaggaagtt tagagtttga cagtgtggtc tcaaaagcac cgagtcaaaa
    59461 aggcaatgaa atgaatcatt tttcaaattg gggcctaact tctgatggct atttaaaacc
    59521 tgacattact gcaccagggg gtgatatcta ctctacctat aacgataacc actatggtag
    59581 ccaaacagga acaagtatgg cctctcctca gattgctggc gccagccttt tggtcaaaca
    59641 atacctagaa aagactcagc caaacttgcc aaaagaaaaa attgctgata tcgttaagaa
    59701 cctattgatg agcaatgctc aaattcatgt taatccagag acaaaaacga ccacctcacc
    59761 gcgtcagcaa ggggcaggat tacttaatat tgacggagct gtcactagcg gcctttatgt
    59821 gacaggaaaa gacaactatg gcagtatatc attaggcaac atcacagata cgatgacgtt
    59881 tgatgtgact gttcacaacc taagcaataa agccaaaaca ttacgttatg acacagaatt
    59941 gctaacagat catgtagacc cacaaaaggg ccgcttcact ttgacttctc gctccttaaa
    60001 aacgtaccaa ggaggagaag ttacagtccc agccaatgga aaagtgactg taagggttac
    60061 catggatgtc tcacagttca caaaagagct aacaaaacag atgccaaatg gttactatct
    60121 agaaggtttt gtccgcttta gagatagtca agatgaccaa ctaaatagag taaacattcc
    60181 ttttgttggt tttaaagggc aatttgaaaa cttagcagtt gcagaagagt ccatttacag
    60241 gttaaaatct caaggcaaaa ctggttttta ctttgatgaa tcaggtccaa aagacgatat
    60301 ctatgtcggt aaacacttta caggactcgt cactcttggt tcagagacca atgtgtcaac
    60361 caaaacgatt tctgacaatg gtctacacac acttggcacc tttaaaaatg cagatggcaa
    60421 atttatctta gaaaaaaatg cccaaggaaa ccctgtctta gccatttctc caaatggtga
    60481 taacaaccaa gattttgcag ccttcaaagg tgttttcttg agaaaatatc aaggcttaaa
    60541 agcaagtgtc taccatgcta gtgacaagga acacaaaaat ccactgtggg tcagcccaga
    60601 aagctttaaa ggagataaaa actttaatag tgacattaga tttgcaaaat cgacgaccct
    60661 gttaggcaca gcgttttctg gaaaatcgtt aacaggagct gaattaccag atgggtatta
    60721 tcattatgtg gtgtcttatt acccagatgt ggttggtgcc aaacgtcaag aaatgacatt
    60781 tgacatgatt ttagaccgac aaaaaccggt actatcacaa gcaacatttg atcctgaaac
    60841 aaaccgattc aaaccagaac ccctaaaaga ccgtggatta gctggtgttc gcaaagacag
    60901 tgtcttttat ctagaaagaa aagacaacaa gccttataca gttacgataa acgatagcta
    60961 caaatatgtc tcagtagaag acaataaaac atttgtggag cgacaagctg atggcagctt
    61021 tatcttgccg cttgataaag caaaattagg ggatttctat tacatggtcg aggattttgc
    61081 agggaacgtg gccatcgcta agttaggaga tcacctacca caaacattag gtaaaacacc
    61141 aattaaactt aagcttacag acggtaatta tcagaccaaa gaaacgctta aagataatct
    61201 tgaaatgaca cagtctgaca caggcctagt cacaaatcaa gcccagctag cagtggtgca
    61261 ccgcaatcag ccgcaaagcc agctaacaaa gatgaatcag gatttcttta tctcaccaaa
    61321 cgaagatggg aataaagact tcgtggcctt taaaggcttg aaaaataacg tgtataatga
    61381 cttaacggtt aacgtatacg ctaaagatga ccaccaaaaa caaaccccta tctggtctag
    61441 tcaagcaggc gctagtgcat ccgctattga aagtacagcc tggtatggca taacagcccg
    61501 aggaagcaag gtgatgccag gtgattatca gtatgttgtg acctatcgtg acgaacatgg
    61561 taaagaacat caaaagcagt acaccatatc tgtgaatgac aaaaaaccaa tgatcactca
    61621 gggacgtttt gataccatta atggcgttga ccactttact cctgacaaga caaaagccct
    61681 tggctcatca ggcattgtcc gcgaagaagt cttttacttg gccaagaaaa atggccgtaa
    61741 atttgatgtg acagaaggta aagatggtat cacagttagt gacaataagg tgtatatccc
    61801 taaaaatcca gatggttctt acaccatttc aaaaagagat ggtgtcacac tgtcagatta
    61861 ttactacctt gtcgaagata gagctggtaa tgtatctttt gctaccttgc gtgacctaaa
    61921 agcggtcgga aaagacaaag cagtagtcaa ctttggatta gacttaccgg tccctgaaga
    61981 caaacaaata gtgaacttta cctaccttgt gcgggatgca gatggtaaac cgattgaaaa
    62041 cctagagtat tataataact caggtaacag tcttatcttg ccatatggca aatacacggt
    62101 cgaattgttg acctatgaca ccaatgcagc caaactagag tcagataaaa tcgtttcctt
    62161 taccttgtca gctgataaca acttccagca agttaccttt aagatgacga tgttagcaac
    62221 ctctcaaata actgcccact ttgatcatct tttgccagaa ggcagtcgcg ttagccttaa
    62281 aacagctcaa ggtcagctaa tcccgcttga acagtccttg tatgtgccta aagcttatgg
    62341 caaaaccgtt caagaaggca cttacgaagt tgttgtcagc ctgcctaaag gctaccgtat
    62401 cgaaggcaac acaaaggtga ataccctacc aaatgaagtg cacgaactat cattacgcct
    62461 tgtcaaagta ggagatgcct cagattcaac tggtgatcat aaggttatgt caaaaaataa
    62521 ttcacaggct ttgacagcct ctgccacacc aaccaagaca acgacctcag caacagcaaa
    62581 agccctacca tcagcgggtg aaaaaatggg tctcaagttg cgcatagtag gtcttgtgtt
    62641 actcggactt acttgcgtct ttagccgaaa aaaatcaacc aaagattgat aacaaaacgc
    62701 aaagagacaa caaatcatcc cttttaacag tcggaccttt ctttgagatt gagctggcag
    62761 atgatcattt cttaacaaaa gttaaagagt tgagcaaagt aaggaacaga cataaagacc
    62821 ataaaagcaa gccagtttgg cttgctttta tggtgttatt gtcgtgtgct acgatgactg
    62881 aaaaagaaca acgaacaatg acctcgaatc accaaaagga cttgatttga tagcttccct
    62941 atgctatact aagcctattc tttcaagaag gactcctttt gtggaaaaga ctgaaaaaaa
    63001 ggacactaat ctatccaaaa actccagatg agtagctgca aacggatgcc tctgctgtga
    63061 tcaaggggat aaagcagact ggcttgtcac tacctgagca ggtgtcaaaa aaagaagctt
    63121 ttggctttga cattcaacat actgagcttt agtattttag aacggagaca cccccagttt
    63181 tgtactcaaa aagcatgaac agttctgctg atgtgacatc tgatttctac gtgcgcagtg
    63241 agccaaaaca gctgctgttt gctatggaaa aagtggcgcc aatcgagatt tttgagaccc
    63301 taggctcaaa aataaggcat agaattccaa aggaagtcgc tgccgtgagc acaacctaag
    63361 aaaaactatt ccaaaagact ttttctgcaa tttgaaatac tgaatttcag tattttttct
    63421 tttgtaacta agagcaatga atgaggtaaa aaaaggcaac aatgtcaaaa aagaaacgaa
    63481 aacactatca ggtttatgat ggattacgct gtgctatgac gctttgtttt atcagtggct
    63541 atgtcaatgc ctttacctat atgacccaag gcaaacgttt tgcaggtgtt cagacaggca
    63601 accttttgtc atttgccatt cgcttgtcag aacagcaact gaaggaggca ctgcaatttt
    63661 tacttcccat gatagttttt atgcttgggc agtcctttac ttatttcatg caccgctggg
    63721 ccactaaaaa agggctccac tggtacttat tatcaagcgt tatcttaaca gggattgctt
    63781 ttggaatggc cctattcacg ccttttcttc cttccaatgt tacggtagca gctttagcct
    63841 tttttgcctc tattcaggtt gatacgttta aaaccttacg aggagctagc tatgctaatg
    63901 tgatgatgac aggaaatatc aaaaatgccg cctatttact aaccaaaggc ttgtacgaaa
    63961 aaaatcacga actaacacat atcggacgca acaccttaat agtcatcttg gcttttgctg
    64021 ttggggtggt ttgttcgacg ttgctttgca tagcttatgg cgagtatgct ttgatgccta
    64081 ttttaatgcc tttactctat gtcaattacc tgttggctca ggaattttat cacattcaaa
    64141 ccaaaatcaa accgattaaa agaacttaac ggacttgtgg agaaagaaag ttcatttagg
    64201 gtaagttttt acttaccctc cgttgatttg ctggttttgc tgccccttaa aatatgatat
    64261 aatagaccat tgatagctgt tacttaacag aaggctgata aaaaaggttc cattagcctt
    64321 gagggttgaa tttaaacagg aaaaaacgaa atcctacact atgcatctca atcgtacttt
    64381 catttgagta tggttctgaa aggaaactat gatgaaaaaa ccattttaca ttacgacacc
    64441 catttattac ccatctggga aattacacat tggttctgcc tatacaacta ttgcttgtga
    64501 tgtcttggca cgctacaagc gtctgatggg ccatgaggtc ttttacctca caggactaga
    64561 tgaacacgga caaaagattc aaaccaaggc aaaagaagct ggtattacac ctcaaaccta
    64621 cgttgataac atggcaaagg atgtcaaagc cctttggcaa ttgctagata tttcctatga
    64681 cacatttatc cggacaacgg acgattacca cgaagaggtc gttgctgctg tttttgaaaa
    64741 attactggcg caagatgaca tttaccttgg tgaatactct ggttggtatt ctgtctctga
    64801 tgaggaattt ttcactgaaa gccaactaga agaagttttt agagatgaag acggtaaggt
    64861 tattggaggg attgctcctt caggtcacca agtagagtgg gtatcagaag aatcttactt
    64921 cctacgcctt agcaagtacg ctgaccgcct cgtagccttc tttaaagagc gacctgactt
    64981 tatccaacca gacggtcgta tgaatgaaat ggtgaaaaac tttatcgagc caggcctaga
    65041 agatttggct gttagccgta ctacctttac atggggtgta cctgttccat cagacccaaa
    65101 acacgtggtt tatgtctgga tcgatgccct cctaaactac gcgacagctc ttggttatgg
    65161 tcaagctaat cacgctaact ttgataaatt ctggaatgga acggttttcc acatggttgg
    65221 aaaagatatc ttgcgttttc atagcatcta ctggccgatc ttactcatga tgcttgattt
    65281 accaatgcca gaccgcttaa ttgcccatgg ttggtttgtc atgaaagacg gtaaaatgtc
    65341 taaatcaaaa ggcaatgtgg tctatccaga aatgttagtg gagcgctttg gacttgatcc
    65401 tcttcgttat tacctcatgc gttctttgcc agttggttca gacgggacct ttacccctga
    65461 agattatgtg ggtcgtatca actatgagtt agccaacgac cttggcaatt tattgaaccg
    65521 taccgttgcc atgatcaaca agtattttga cggcacggtg ccagcttatg ttgacaatgg
    65581 aactgccttt gatgctgact tcagtcaact gattgatgct cagctagcag actaccataa
    65641 gcacatggaa gctgtagact acccacgcgc cttagaagcc atttggacca tcattgctcg
    65701 taccaacaaa tacatcgatg aaaccgctcc ttgggtgctc gctaaagaag atggtgacaa
    65761 agctcaacta gccagtgtca tggcacactt ggcagctagc ttgcgcgtgg tcgctcatgt
    65821 gatccaaccc tttatgatgg aaacatcagc tgctatcatg gcccagcttg gcttagcgcc
    65881 tgtttctgac ttatcaacac ttgctctagc agattttcct gccaatacaa aggtcgttgc
    65941 caagggcaca ccgattttcc cacgtcttga catggaagct gagattgact acattaaagc
    66001 ccaaatgggt gacagctcag ctatttccca agaaaaagaa tgggtccctg aagaggttgc
    66061 gctcaaatct gaaaaagacg tcatcacatt tgaaaccttt gatgctgtgg aaatccgtgt
    66121 ggcagaagtc aaggaagtgt caaaagtgga aggttcagaa aaattgctgc gctttagagt
    66181 agatgcagga gacgggcaag accgtcaaat cttgtcaggt attgccaaat gctaccccaa
    66241 cgagcaagaa ttagtgggta aaaaactaca aattgttgct aacctgaaac cgcgtaaaat
    66301 gatgaaacaa tacatcagcc aaggcatgat tttgtcagca gagcatggag atcagctaac
    66361 ggtcttaacc gttgattcct ctgttccaaa tggaagtatc attggctgat gctgacctat
    66421 tattcaaaaa cctaagcaag aatatgtgct taggtttttt gattgccttc aagaaagatg
    66481 aataacgaga tgggtgatat ggcatcaaaa gcataactat cctaggacct gtcctagtaa
    66541 gattgctgtg cttttgacaa gtgaacagag aggtcttaaa ctatatcttg tcataaaaaa
    66601 gattgcaaaa cactatatct tgtgttatta tggtttttat aaaagttaag cgtgcttaac
    66661 tttagccaaa caaaagagga cactagagcg tgtcccacaa gacagttgag cccttcgctt
    66721 ttttagtaga gtaggagtgc tcaagtcaaa aagagacagc ccatcaaacc attgctgttt
    66781 agcaggtctt tttgtgttgc tcaaaaaaca gttcctgtct gtttggcgtc tagtgatgac
    66841 ttaggcttag cgctactaga cttttgtttg aaaaatacct caagccttga aggagacaaa
    66901 tgacccaaca ttattatgaa cgttcgcaat ccccaattga atacgcccta tctgaaacac
    66961 aaaaacagtt gcgctccatc aattggaatt accttaatga tgacaaagat ttggaagtgt
    67021 ggaaccgtgt cactcaaaat ttttggttgc ctgaaaaggt tcctgtttct aatgatttaa
    67081 attcttggcg ctccttgggt gaggattggc aacagctcat cacaaggacc tacactgggt
    67141 tgaccttact agatacagta caagcaacgg ttggtgatgt ggcacaaatt cagcattcac
    67201 aaaccgacca cgaacaagtc atctatacga actttgcctt tatggtgggg attcatgccc
    67261 gctcttacgg gacgattttt tcgacccttt gcagcagcga gcaaatcgaa gaagcacatg
    67321 aatgggtggt ctcaacacag agcttgcaag accgttcccg tgtcttgatt ccttactata
    67381 cgggtgatga ccctttgaaa tcaaaagtag cagcagctat gatgccagga tttttgctgt
    67441 atggcggctt ttaccttcct ttttacctat cagcacgtgg taaaatgcca aacacctctg
    67501 atatcatccg cctgatttta cgcgacaaag ttattcacaa ctattacagc ggttacaaat
    67561 accaacaaaa agtagcacgt cttagcccag aaaaacaggc tgaaatgaag gcctttgtct
    67621 ttgacttgct ctatgaactc atcgaccttg aaaaagctta cttgagagaa ctgtatgcag
    67681 gctttgattt ggcagaagat gctatccgtt ttagccttta taacgctgga aaattccttc
    67741 aaaatttagg ttatgattcg cccttcacag atgaagaaac ccgtatctca cctgaagtat
    67801 ttgctcagct atcagcgcgt gcagacgaaa accatgactt tttctcaggc aatggctcat
    67861 cttatgtgat ggggatcacc gaagaaacca cagatgacga ttgggagttt taagaaatgg
    67921 cagagctgat tattgtgtat ttttcatcta agtccaataa cacccatcgc ttcgtccaaa
    67981 aattaggcct gcctgctcag cgcataccag tagataatag gccacttgaa gtttcaaccc
    68041 attacctttt gattgtgcct acctatgcag ctggtggttc agatgccaaa ggagccgttc
    68101 ctaaacaagt gattcgtttt ttgaataatc ctaacaatcg taaacattgt aagggagtga
    68161 tctcttcagg aaataccaat tttggcgata cctttgcctt agcaggccct atcatttctc
    68221 aaaaattgca ggtcccccta ttacaccaat ttgaactatt aggcactgct acagatgtca
    68281 aaaaggtaca ggcgatcttt gcccgcttaa agcatcacac acacgacaag caaaaacaaa
    68341 tcaacaacct gattacagaa aggacacacc catgtcacaa accaatgcgt catacctctc
    68401 attaaatgca ctgacccgtt ttaaaaaacc agacggcagc taccattttg atagcgataa
    68461 agaagctgtt aggcgctacc tggaagaaca tgtctctccc aatcaaatgg cttttaactc
    68521 actagaagat aagctagctt acctcatcaa tgaagggtat tatgagcagg ccatttttga
    68581 tgcctatcct aacgacctta tcaaggaggc ctttcattat gcctatcaac aaggctatcg
    68641 ctttttaaat ctcatgggcg ctatgaaatt ttaccagtcc tatgccttaa aaaccttgga
    68701 cggtaagcag tatctagaaa catttgaaga ccgtgctgtg atgaatgcac tctttttagc
    68761 tgatggagat caaacctttg tctttgacgt gattgatgcc atcttgcacc gacgctttca
    68821 accagctacc ccaacgtttt taaatgccgg aaaaaaacgc cgtggcgaat acatttcgtg
    68881 ctatcttctt cgtgtcgaag acaatatgga atctatctct cgcgccatta gcaccagctt
    68941 gcaactctca aaaagaggag gcggagtcgc cttgtgcctc accaacctca gagaaatcgg
    69001 agcaccgata aaagggattg aaaatcaggc aacaggcatc gttcctgtca tgaaactact
    69061 agaggactct ttttcctatg ccaatcaact aggtcagcgc caaggagcag gtgcggttta
    69121 ccttcatgcc catcatcctg aggtcttgac ctttttagac accaaacgtg aaaatgccga
    69181 tgagaaaatt cgcattaagt ccctagcatt gggacttgtg atcccagata ttaccttcca
    69241 gttagccaaa gaaaacaaag acatggctct cttttcaccc tatgatatca agcgtgccta
    69301 tggcaaggac atgtcagaca tctccatcac agaggaatac gataaactgc tagccaatcc
    69361 agccatcaaa aagacctaca tcagtgctag aaagttcttt cagttaattg cagagctaca
    69421 ttttgagtca ggctaccctt acctcttgtt tgatgacacg gtcaataagc gcaatcctca
    69481 tgccaaaaaa ggcagaatcg tcatgtctaa cctgtgttct gaaatcgctc aagtcagcac
    69541 cccaagcact ttcaaagaag atttgagttt tgaaaccatt ggagaggata tctgctgtaa
    69601 tttagggtct atcaatattg cccaggctat ggcagatgcg ccacactttg aacagctcat
    69661 cacaacgagc atcagagcac ttgatcgtgt ctctagagtg tctgacctga attgtgcgcc
    69721 ttctgttgag acaggcaatg cagccaacca tgcagtagga ttaggcgcca tgaacctaca
    69781 tgggtttttg gcaaccaacc acatctacta tgatacaaaa gaagccgttg actttacaga
    69841 tctgtttttc catgccatgg cctattatgc ttttaaagcc tcttgccaat tagccaaaga
    69901 aaaaggcgct tttgcaggat tttcgttatc aacctatgca gatggcactt attttgccaa
    69961 gtatttgcaa gaagatgcca agccacaaac agccaaggtt gccacattgt tgcaagatta
    70021 cggcttcaca ttgccaacgg tggcggattg gcaagcccta gtagcagata tcaagcagtt
    70081 tggtctagcc aatgcgcact tgctagcggt tgctccgaca ggctccatca gctacttatc
    70141 atcctgcact ccgagtctac aaccagtcgt tgctcctgtt gaggtccgaa aagaaggtag
    70201 cctgggcagg atttacgtcc ctgcctatca gattgaccag gctaactatg cttactatga
    70261 acgtggtgcc tatgaggtcg gcccaaaagc catcatcgac gttgtagctg ctgcccaaaa
    70321 acacgttgac caagccatct cattgaccct gtttatgacc gatcaagcga ccacacgtga
    70381 ccttaaccgc tcctatatcc aagcttttaa gcaaaactgt gcatcgatct attatgtgcg
    70441 agtgaggcaa gacgtcctag caggcagcga acagtatgac gaggatagtc ttgtgacagc
    70501 tcctggagct agtgatgaga ccaccacaga gtgtcaaagc tgtatgatct aacagctact
    70561 agaaagctct cgtgtcccct atcacatgca taggatcagt gcactcgacc tttcaagaca
    70621 agcaagcatc agctcttgct tgtctttttt tggccttaaa gcccgttagt ctgctgctat
    70681 gcgaggcttt ttttgagcat tagaacgtca aaaaaaagga catggagtcc ttttttggtg
    70741 atcggtgttg aggccgtcac aaactgccct tgaaatacgc ttctatgtgg agcttttttt
    70801 ggtcttgtga cacgtaagct ccaacgacct ccagctgaca gcctcttgga aacaagagct
    70861 caacctctga aggcacagcc gaataaggct cgacaaaggc tgccttggcc ccttttttga
    70921 cacagatgcg cacctccacc ggtcgatggg tcatggcgcc gtttttcaag gctgtcgtgc
    70981 tcataaaact gtgcttggtg tagcgtgtgc caagcttgat cttacaaagg atatgagggt
    71041 caaactgatg gttacggtag taagacgtga gatcagcgtg tgaaacaccg atatcacgca
    71101 aaaacgtctc gtacacatag cggtatacca caatgttcca cgggatgact agccggtgag
    71161 tcgcagcatc gagctgggcc acctgatccc ttagctcagg cgtgagttgg ctgagctcac
    71221 ctttggtctt gtctaggctg gtgttgatag gggctgcctg ctggcaggta taagcagcca
    71281 tcgcctcttg ctcagccctt gggtagacga gacccttgcc ccagcgcttt ttaatcagtg
    71341 tctcagcgcc cttttgatcc gaaaaggtaa aataagcagg cgtcccctca caaaccgccc
    71401 gctcatagtg ctcacagaca aaaagctcga ccaagccctc agtttgccag actagactaa
    71461 aacagctaag aagaaggatc atagccagtt gatagcgctt ttttagcata gttccccctg
    71521 acaccagccc aaaaggctga cccttcccct aggccttttg gctagctcat gataccacca
    71581 gtttctcaga caggctaatc tttttgtcat ctctttctcc ctaaacagac tctcttatca
    71641 tgatattatg ctattatgag cgatttgtca atccatagca accgtttttt agccagataa
    71701 gtaagctttt gacctagagg cagacaagag tggcagtacc tgccgtttgc tgtggtggct
    71761 aataaggact aacataaaat atcaaacgct ttcaaaaaca gctcctaagc ttaaaaaagc
    71821 tgatgaacaa ttttttgagt ttaagagaga ttgccatgat tttctaatca tttgatgttt
    71881 aaaatgataa aaacatgaac ttgaaatgaa agaatacgag tagtaaacta aaaatatatc
    71941 aaaaataatg aaatggagtg attttatgaa aacaaaatca aaacgctttt taaacctagc
    72001 aaccctttgc ttggccctac taggaacaac tttgctaatg acacgtcccg taaaggcgga
    72061 ggtgtcagaa attgggcacg atcacgtaac aatcagttct aatggtcaaa cagatgaggg
    72121 tgctgcatat ggtagaggcc atgatgatgg aagtaagttt ggatatgaag caggattaca
    72181 atcgagttgg aatgagtctg aacccccatc atcagataaa atacctgagc ctagtgtgaa
    72241 tccatatgaa agtagtaatg aacaagatag agaggattat aaagaaggtt ttcgtgatgg
    72301 atatcctggg ggctatgtcg caggctggag aaaaactcat ccaatagaag ctactcttca
    72361 atatttatgg tatacggtat ctagttggtt tgaaagctta tttaataata gtaagtagat
    72421 atttgtttgt tgattaaagt tacttaaggt aaaataaaat gtctgtcagc aacttcgtgc
    72481 tagtggcttt tgttttacct ttcccacact aatctgctat aattgggata atccctttag
    72541 tagtcaggcc acctctgata ggggtggcct tttttgggtt ttagtgaccc gtgtgttgaa
    72601 aaaggagcat ttatgccaga aaagaaagtg ttgcccgtcc taagcatggt ggtgagtttt
    72661 gtctgcctgc ttgatcatat cttctttttt gggctctttg tcaactgtag tgggtgactt
    72721 atagctaaac acgtgaggag acgagactcg tcttctcttt tgttatgttc aaagcgaggt
    72781 aaatgcgttt tttaaagtta tcaaagttcc gaaagccaaa agcattgcgt ttgatgtgtt
    72841 tgatgagttt attggttgct tctagtttgg cgtttgaaca aggaagctca atgacattag
    72901 tgacatagtt tttgtatcgc atcagggtgt tcaaagccgt tttaaagatg ttattgagca
    72961 gaagtaacac cttttgaatt aagtcaaaaa atgctcagta ttcttctctt gtaagtgaaa
    73021 aaggagtaac tgataaaggt catagtaatc ttttaattca ggaacgggat taaatagttt
    73081 attgagacat tgtttaggag ttagcgtttc cctaaaggtt ctagagtaga aggcgttgag
    73141 cgagagtttt cggctacctt ttgaatgagt tgacagtagt atttaagaac tctgtattcc
    73201 agagactttt tgtcatattg cttcatgatg tgaatccggg tttgactgag cgctctgttc
    73261 atgtgctgaa caataggaaa cgatctagga cgatgttagc ttgaggaaat agctttttaa
    73321 tgagcgaaat atagcttcct gacatatcaa cggtaacgat tttgacactg tttctagcct
    73381 ctttagagta tctaaagaag tgatttcgaa tggttgtttg tcgccggttg tccagtatgg
    73441 agatgatttt tttggactca aaatcttgag cgataaaggc gagttcgccc ttttgatagg
    73501 aaaatccatc ccaagacaac acctcgggta gtttagagaa gtcttccttg attacaaact
    73561 ggttgagttt tcggtagacg gtagaagatg atatagccga ctgttgagcg atgttagtga
    73621 gagctcctcg attaaccaag gtttgtgcga ctttttgtct gaggggctca gagatctgac
    73681 agtttttttg aaccagactt gtttccgcta cagccacttt tcgacaggat ttgcattgag
    73741 atcttctctt ttttaaacga atgagactag gaaaaccacc aatttcgata aaggggattt
    73801 tagaatgttt ctgggagtcg tatttaatct gaactccttt acagtgttta cacttaggta
    73861 gggatagtca agtgtggcga agacttcgat aagtgtctcg cgttgaaaaa ccttgttcaa
    73921 ggtgatgtgt ttatctttaa ttctaatcaa ttctgtagta tgatagagtt gtctcatatg
    73981 attctttcta atgatggttt ggtcgctttg cattataagt catatgggat tttttgctac
    74041 ttatagttac ctctacttgc cgtttgtagc aagtagtcta gcattgtagg tcttgtataa
    74101 aacaaaagca agtgttcgct ggctgggctt gtagtagcag taacagtata atatgaaccg
    74161 acagctattc cctattaaaa tagacgcaaa tgtccacgct ttcactgtag ttgctagtaa
    74221 ggtctaacac aaaacatcaa aggctttcaa aaaaactcct aagcgcaaaa aggcggacta
    74281 acagccccaa gcaagcactt tctgaggtaa taagagattg ccatgatttt ctaatcattt
    74341 gatgtttaaa atgataaaaa aatgaacttg aaatgaaaga gtgcgagtag taaactatga
    74401 ccgtatcaaa aataatgaaa tggagtgatt ttatgaaaac aaaatcaaaa cgctttttaa
    74461 atctagcaac cctttgcttg gccctactag gaacgacttt gctaatggga caacccataa
    74521 aggcggaggc agtagtatct gatttgaatg atgcagtctc ttcgatggga gatacagcag
    74581 aggaaactga cgtcaatatc gatggtcaag aagacgaaga agacgaagaa gacgatgctt
    74641 atatgcaagg gagaagactg ggagagaaag aggggtatga agctggtcaa aaagacaacg
    74701 ctcccagatt atttgacagt agtacgctag agccccctac taatccttac gaggatgcgg
    74761 gtgatacatt gaatagtaat tggtttaaaa gtggttacag cgatgcttat ggtcaagcat
    74821 atgagcaggg atggaatgat actcatccga tacaatcaac tttaatttgg ctgtggggga
    74881 tagtaaggaa cttatttaga ttttagattt aggtgacggt taccaacaac aaagtaatta
    74941 atattacgtt ataaaaagct agtatcgctt ttgaggatag tgttatttac tcttttgaga
    75001 tatttgaaat actataacac taaaatgaat ccttttatca caagcatttc ccccactaat
    75061 ctgctataat tgggatagtc cctttagtag tcaggccacc tctgataggg gtggcttttt
    75121 tgggttttag tgacccgtgt gttgagaaag gagcatttat gccagaaaag aaagtgttgc
    75181 ccgtcttaag catggtggtg agttttgtct gcctgcttga tcatatcttc ttttttgcta
    75241 cttatagtta tctctacttg ccgcttgtag caagtagtct agctcttatc agtctggtgc
    75301 aaaacaaagg caggcaaaag gtttggtcgc tagctgggct tatcttagct gctctagccg
    75361 tggcagtagt ggcctatgcc tactatgacc caaccgtcat cccctattaa ccttatctcc
    75421 tctttgcacg caatcagcag cacaaaaagc acccttacag gtgcttagat tgaaaaaggt
    75481 cttttgtgat actttttctg aggcggtgcg gacggtagcg acttcctttg gaattccctg
    75541 acttattttt gagacctaag gtctcaaaaa tcccgagtgg cgtcctctcg tattgacgcc
    75601 actttcacca tgacaaaaat catctgtttt ggctcccttt gtgtgtaaaa atcggaaacc
    75661 gtatgtcaca tcagcagagc tgtttatgct ttttgaacat agaactaggt ttgtctaggt
    75721 tctaagcacc tgtaagggtg ctttttgtac tttagccaac ctcctcatag cctagcaaac
    75781 cctcactgaa acagtccctt aaagttccac caagcgtctt gaaaaacatc gcgtacttta
    75841 tcccgccatg ttttttgttc ctctcttaga cgagaatttt caaaaaggtt ttcggcatat
    75901 tcttcatcaa atgctttata ttgttcattt ttgagtgttt ctgaatcaat aaaagtcatc
    75961 attccagcta aaaatccatc atctccttga aaataaatag taaattcttt tggaggttgc
    76021 gacgttgaga tatcgagagt gactgtagta gtcactgaat tgctgtttcg ttgtttcgtg
    76081 tttttatcaa tccatgcaga ccatcctgga ggaagtgtaa cagtgacttt attacctgta
    76141 atgtgaacag gaatttcatt tctgttatca tctaaaactt tttgttgtct tggaagaggt
    76201 acccaagaac tctcctccgc tctgacgcta ctagttaccc cgatggtaaa gagtgccagt
    76261 agcaaagtta atgtcttttt cattttttcc tctccttttg ctcatttttt aagacaaggg
    76321 ttattatacc aattcttgcg ctaaaaagat agggagttca tgtcttgtgg ttgggaaatg
    76381 gcgcgactat tcttggtagg atcagctagt ttttttccta gtcagtcgct agctgttgcc
    76441 attttacctt taaaggtcgt ttgggtatta tgtcatattt tatgagcggt tttatatttg
    76501 gtatcaatat cttatagggc aattgctcat atttattgaa agctctta
//
LOCUS       DATUYR010000002        67429 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.002, whole genome shotgun sequence.
ACCESSION   DATUYR010000002 DATUYR010000000
VERSION     DATUYR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67429
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.002"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1547
                     /gene="shr"
                     /locus_tag="VCI33_000074"
     CDS             <1..1547
                     /gene="shr"
                     /locus_tag="VCI33_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEP2831034.1"
                     /translation="TKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVSPKVRALI
                     GQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVSIEEYQQL
                     LQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLTGEFSLKD
                     PGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVEKGLRALNQKQLRDG
                     IYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKMLGYLGELS
                     YFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKLIEQAKKD
                     GLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENSHLTSTDQ
                     LKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKAEKLKQLV
                     RDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIVALGFIIG
                     RKTLFK"
     gene            1744..2619
                     /locus_tag="VCI33_000075"
     CDS             1744..2619
                     /locus_tag="VCI33_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEP2831035.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDNQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            2603..3487
                     /gene="isdE"
                     /locus_tag="VCI33_000076"
     CDS             2603..3487
                     /gene="isdE"
                     /locus_tag="VCI33_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEP2831036.1"
                     /translation="MIKRCKGIGLALMAFFLVACVNQHHKTAKESEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSANPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHS"
     gene            3489..4511
                     /locus_tag="VCI33_000077"
     CDS             3489..4511
                     /locus_tag="VCI33_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP2831037.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            4508..5344
                     /locus_tag="VCI33_000078"
     CDS             4508..5344
                     /locus_tag="VCI33_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831038.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDLQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            5341..7104
                     /locus_tag="VCI33_000079"
     CDS             5341..7104
                     /locus_tag="VCI33_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEP2831039.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLLLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQAIERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLLQETINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLNTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            7097..8767
                     /locus_tag="VCI33_000080"
     CDS             7097..8767
                     /locus_tag="VCI33_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831040.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     ETCGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGSLKEVNKK"
     gene            8764..9357
                     /locus_tag="VCI33_000081"
     CDS             8764..9357
                     /locus_tag="VCI33_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEP2831041.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPIVLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            9354..10034
                     /locus_tag="VCI33_000082"
     CDS             9354..10034
                     /locus_tag="VCI33_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP2831042.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            9971..11425
                     /locus_tag="VCI33_000083"
     CDS             9971..11425
                     /locus_tag="VCI33_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831043.1"
                     /translation="MERLGGADLDYCYHNHHDTAMISAEQLVFTYHDQKNPACQVSTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQADRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            11800..13815
                     /gene="recG"
                     /locus_tag="VCI33_000084"
     CDS             11800..13815
                     /gene="recG"
                     /locus_tag="VCI33_000084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEP2831044.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENRSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAQNIRKKEVYD"
     gene            14108..15010
                     /locus_tag="VCI33_000085"
     CDS             14108..15010
                     /locus_tag="VCI33_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831045.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
     gene            15023..15727
                     /locus_tag="VCI33_000086"
     CDS             15023..15727
                     /locus_tag="VCI33_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831046.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(16076..17041)
                     /locus_tag="VCI33_000087"
     CDS             complement(16076..17041)
                     /locus_tag="VCI33_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEP2831047.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            17113..18501
                     /locus_tag="VCI33_000088"
     CDS             17113..18501
                     /locus_tag="VCI33_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2831048.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGIAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDIEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            complement(18624..19076)
                     /locus_tag="VCI33_000089"
     CDS             complement(18624..19076)
                     /locus_tag="VCI33_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEP2831049.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            19307..20521
                     /locus_tag="VCI33_000090"
     CDS             19307..20521
                     /locus_tag="VCI33_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP2831050.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISISLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            20739..21521
                     /gene="codY"
                     /locus_tag="VCI33_000091"
     CDS             20739..21521
                     /gene="codY"
                     /locus_tag="VCI33_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEP2831051.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASIIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            21668..22222
                     /locus_tag="VCI33_000092"
     CDS             21668..22222
                     /locus_tag="VCI33_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEP2831052.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            22666..22984
                     /locus_tag="VCI33_000093"
                     /pseudo
     CDS             22666..22984
                     /locus_tag="VCI33_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
     gene            23216..23518
                     /gene="gatC"
                     /locus_tag="VCI33_000094"
     CDS             23216..23518
                     /gene="gatC"
                     /locus_tag="VCI33_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEP2831053.1"
                     /translation="MKISEEEVRHVAKLSKLSFLESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDIAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            23518..24984
                     /gene="gatA"
                     /locus_tag="VCI33_000095"
     CDS             23518..24984
                     /gene="gatA"
                     /locus_tag="VCI33_000095"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEP2831054.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVCIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            24984..26423
                     /gene="gatB"
                     /locus_tag="VCI33_000096"
     CDS             24984..26423
                     /gene="gatB"
                     /locus_tag="VCI33_000096"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEP2831055.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIRLTPDNLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            26420..26635
                     /locus_tag="VCI33_000097"
     CDS             26420..26635
                     /locus_tag="VCI33_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831056.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTVYKKTIIYAKI
                     RLTGIALEPSGVSDVYLMNYHVCLHYY"
     gene            26854..27093
                     /locus_tag="VCI33_000098"
     CDS             26854..27093
                     /locus_tag="VCI33_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP2831057.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            27485..28192
                     /locus_tag="VCI33_000099"
     CDS             27485..28192
                     /locus_tag="VCI33_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP2831058.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFMWLIDPDREKQYIDNGSVTVVEFEDGQFTIKTIGDM
                     SYRYRGREIIEAMSDEA"
     gene            28182..28925
                     /locus_tag="VCI33_000100"
     CDS             28182..28925
                     /locus_tag="VCI33_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEP2831059.1"
                     /translation="MKHNHFKLLLQTLVVLLFVGGIYLFIKPEKQATKTPSSQQASSQ
                     KVVKNSKESTGLPKVSPKDWELILVNRDHMTEELSPELVDINGISVDKRIEQATSDFL
                     AAAQAIDPQEHLISGYRSVAYQAELYQSYIKKEMANDPTLTQEAAEALVQTYLQPPGA
                     SEHHTGLAIDMSTVDTLNASDPSVSKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            28922..29491
                     /locus_tag="VCI33_000101"
     CDS             28922..29491
                     /locus_tag="VCI33_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP2831060.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            29626..30660
                     /gene="hrcA"
                     /locus_tag="VCI33_000102"
     CDS             29626..30660
                     /gene="hrcA"
                     /locus_tag="VCI33_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEP2831061.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKIFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDSSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            30663..31235
                     /gene="grpE"
                     /locus_tag="VCI33_000103"
     CDS             30663..31235
                     /gene="grpE"
                     /locus_tag="VCI33_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEP2831062.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            31416..33242
                     /gene="dnaK"
                     /locus_tag="VCI33_000104"
     CDS             31416..33242
                     /gene="dnaK"
                     /locus_tag="VCI33_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEP2831063.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAVAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            33523..34659
                     /gene="dnaJ"
                     /locus_tag="VCI33_000105"
     CDS             33523..34659
                     /gene="dnaJ"
                     /locus_tag="VCI33_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEP2831064.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            34879..35670
                     /locus_tag="VCI33_000106"
     CDS             34879..35670
                     /locus_tag="VCI33_000106"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEP2831065.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            35747..36181
                     /locus_tag="VCI33_000107"
     CDS             35747..36181
                     /locus_tag="VCI33_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP2831066.1"
                     /translation="MEYDKINQYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            36182..37156
                     /locus_tag="VCI33_000108"
     CDS             36182..37156
                     /locus_tag="VCI33_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEP2831067.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            37217..37441
                     /locus_tag="VCI33_000109"
     CDS             37217..37441
                     /locus_tag="VCI33_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP2831068.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            37643..38614
                     /gene="fabK"
                     /locus_tag="VCI33_000110"
     CDS             37643..38614
                     /gene="fabK"
                     /locus_tag="VCI33_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEP2831069.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            38639..39577
                     /gene="fabD"
                     /locus_tag="VCI33_000111"
     CDS             38639..39577
                     /gene="fabD"
                     /locus_tag="VCI33_000111"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEP2831070.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            39567..40301
                     /gene="fabG"
                     /locus_tag="VCI33_000112"
     CDS             39567..40301
                     /gene="fabG"
                     /locus_tag="VCI33_000112"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEP2831071.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            40316..41548
                     /gene="fabF"
                     /locus_tag="VCI33_000113"
     CDS             40316..41548
                     /gene="fabF"
                     /locus_tag="VCI33_000113"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEP2831072.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMVDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            41550..42050
                     /locus_tag="VCI33_000114"
     CDS             41550..42050
                     /locus_tag="VCI33_000114"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEP2831073.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNSTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            42047..42469
                     /gene="fabZ"
                     /locus_tag="VCI33_000115"
     CDS             42047..42469
                     /gene="fabZ"
                     /locus_tag="VCI33_000115"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEP2831074.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            42501..43865
                     /locus_tag="VCI33_000116"
     CDS             42501..43865
                     /locus_tag="VCI33_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEP2831075.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAH
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAISQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            43874..44740
                     /locus_tag="VCI33_000117"
     CDS             43874..44740
                     /locus_tag="VCI33_000117"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEP2831076.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            44737..45507
                     /locus_tag="VCI33_000118"
     CDS             44737..45507
                     /locus_tag="VCI33_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEP2831077.1"
                     /translation="MTDVSRILKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(45740..47017)
                     /gene="serS"
                     /locus_tag="VCI33_000119"
     CDS             complement(45740..47017)
                     /gene="serS"
                     /locus_tag="VCI33_000119"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEP2831078.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRSYMGGETVISPK"
     gene            complement(47409..47768)
                     /locus_tag="VCI33_000120"
     CDS             complement(47409..47768)
                     /locus_tag="VCI33_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEP2831079.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGIGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(47882..48793)
                     /locus_tag="VCI33_000121"
     CDS             complement(47882..48793)
                     /locus_tag="VCI33_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP2831080.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(48810..49619)
                     /locus_tag="VCI33_000122"
     CDS             complement(48810..49619)
                     /locus_tag="VCI33_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEP2831081.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAATEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(49708..50700)
                     /gene="manX"
                     /locus_tag="VCI33_000123"
     CDS             complement(49708..50700)
                     /gene="manX"
                     /locus_tag="VCI33_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEP2831082.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            complement(51052..51864)
                     /locus_tag="VCI33_000124"
     CDS             complement(51052..51864)
                     /locus_tag="VCI33_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2831083.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKIIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            52128..53588
                     /locus_tag="VCI33_000125"
     CDS             52128..53588
                     /locus_tag="VCI33_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEP2831084.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            53742..54203
                     /gene="tsaE"
                     /locus_tag="VCI33_000126"
     CDS             53742..54203
                     /gene="tsaE"
                     /locus_tag="VCI33_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEP2831085.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            54178..54702
                     /locus_tag="VCI33_000127"
     CDS             54178..54702
                     /locus_tag="VCI33_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2831086.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVAAAAKETDFITGVETILEATP
                     HELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQVEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            54711..55994
                     /locus_tag="VCI33_000128"
     CDS             54711..55994
                     /locus_tag="VCI33_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP2831087.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(56105..56461)
                     /locus_tag="VCI33_000129"
     CDS             complement(56105..56461)
                     /locus_tag="VCI33_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEP2831088.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(56458..56877)
                     /locus_tag="VCI33_000130"
     CDS             complement(56458..56877)
                     /locus_tag="VCI33_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP2831089.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQLATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEKDGISIQYTTHEPNFPVLEKLARQINQEVSS"
     gene            56949..57674
                     /locus_tag="VCI33_000131"
     CDS             56949..57674
                     /locus_tag="VCI33_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831090.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            57677..58711
                     /locus_tag="VCI33_000132"
     CDS             57677..58711
                     /locus_tag="VCI33_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831091.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGYAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAILLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNVLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            58775..59566
                     /locus_tag="VCI33_000133"
     CDS             58775..59566
                     /locus_tag="VCI33_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEP2831092.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            59566..60201
                     /gene="trmB"
                     /locus_tag="VCI33_000134"
     CDS             59566..60201
                     /gene="trmB"
                     /locus_tag="VCI33_000134"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEP2831093.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            60266..60352
                     /locus_tag="VCI33_000135"
     tRNA            60266..60352
                     /locus_tag="VCI33_000135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60300..60302,aa:Ser,seq:gga)
     gene            60460..60996
                     /gene="rimP"
                     /locus_tag="VCI33_000136"
     CDS             60460..60996
                     /gene="rimP"
                     /locus_tag="VCI33_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEP2831094.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            61171..62328
                     /gene="nusA"
                     /locus_tag="VCI33_000137"
     CDS             61171..62328
                     /gene="nusA"
                     /locus_tag="VCI33_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEP2831095.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV"
     gene            62344..62640
                     /locus_tag="VCI33_000138"
     CDS             62344..62640
                     /locus_tag="VCI33_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEP2831096.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            62633..62935
                     /locus_tag="VCI33_000139"
     CDS             62633..62935
                     /locus_tag="VCI33_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEP2831097.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAVADAGFSKKMRTLME"
     gene            62955..65816
                     /gene="infB"
                     /locus_tag="VCI33_000140"
     CDS             62955..65816
                     /gene="infB"
                     /locus_tag="VCI33_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEP2831098.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            66022..66372
                     /gene="rbfA"
                     /locus_tag="VCI33_000141"
     CDS             66022..66372
                     /gene="rbfA"
                     /locus_tag="VCI33_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEP2831099.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDKKD"
     gene            complement(66425..67426)
                     /locus_tag="VCI33_000142"
     CDS             complement(66425..67426)
                     /locus_tag="VCI33_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP2831100.1"
                     /translation="MKKTLKSTLILLGLTLAIGASTQVDASSRSWKSWFIEKYFWLKG
                     DKGYYSEQDETKFDQYVNSAGEASNKGFDLKEEQVNGTLVTKTVDNMQVHTWNENPSN
                     KANQKVIFYLHGGSYLNNPTTYHIDMLKTLSTSLDAKIILPIYPKAPTYSYSDAMPKL
                     VDLYRDTLKETTAQNITIMGDSAGGGLALGLALALKNESLKQPKDIVLLSPWLDVTMS
                     NPDIESYESNDPILSAWGLRKVGEIWARRDPNNTKHPYVSPINGDVSGLAPITMFVGT
                     HEIFYPDVKKFDDILTAKGHQHELIVADKMNHVYPIYPIEEAKTAQYQIIDTIQKAY"
BASE COUNT        20798 a        11635 c        14338 g        20658 t
ORIGIN      
        1 taacaaaact tgattttttc aataacttgc ttactcaggt tgaagaatca gtatttccag
       61 atgttgaaac gcttaattta gatgtgaagt tcaatcagat aaaaagtgtg agtccaaaag
      121 taagagctct tatcggacaa cacaaactga ctccacaaaa acatattgca aaacttgaag
      181 cttccttaga tggcgaaaaa ataaaatatc atcaagcttt cagtctttta gatttgtatt
      241 attgggagca aaaaacaaat tctgccattg ataaagaact agtgtctatt gaagaatatc
      301 aacaattgtt acaagaaaaa ggttcagata ctgtctcttt acttaatgat atgcaagtcg
      361 attggagtat tgtgattcag ttgcaaaaaa aagcttccaa cggacagtat gtgacggttg
      421 acgaaaagct tctctcaaat gatccgaaag atgacttaac gggagagttt tctttaaaag
      481 atccaggtac atatcggatt cgcaaagctt taataacaaa gaaatttgct actcaaaaag
      541 aacatatcta tttgacatct aatgatatcc ttgtggcgaa aggaccacat tcacatcaaa
      601 aagatttagt tgagaaaggc cttagagcat taaatcaaaa acaattgcgt gatggtattt
      661 actatttaaa tgccagcatg ttaaaaactg acttagcatc tgagtccatg tcaaacaagg
      721 ctattaatca tcgagtgact ttggtagtta aaaaaggtgt ttcctattta gaagttgagt
      781 ttagagggat aaaggttggt aaaatgttag gctaccttgg tgaattgagc tatttcgtag
      841 atggttacca aagagattta gctggtaaac cagttggtcg aacaaaaaag gcagaggttg
      901 tgtcttattt cacggatgta actggcctac cattggcaga tcgttatgga aaaaactatc
      961 caaaagtgct gcgtatgaaa ttgattgaac aagcgaagaa agacggactt gtgccattac
     1021 aggtctttgt gcctatcatg gatgccattt caaaagggtc tggccttcaa accgttttta
     1081 tgcgtttaga ctgggcaagc cttacgacag agaaggcaaa ggttgtcaaa gaaactaata
     1141 atccacaaga aaatagccat ctaacttcaa cagatcagtt gaaaggacct caaaatcgtc
     1201 aacaagaaaa aacacctaca agtcctcctt cagcagctac tggtattgct aacttaactg
     1261 atctcctggc taaaaaagca accggccaat caactcaaga aacttctaag acagatgata
     1321 ctgataaggc agagaaattg aaacagttag tgcgtgacca tcaaacatca attgaaggta
     1381 aaacggcaaa agatactaag actaaaaaat ctgataagaa acatcgttcc aatcaacaat
     1441 caaatggtga agaaagtagc tctcgttatc acttaattgc aggtctatct agctttatga
     1501 tcgtagctct gggattcatt attggtcgaa agacattatt taaataagaa acttagtacc
     1561 aagtgaaata acaaaaaagc tagtcagcaa gagctaaaaa catacctatt tccgacagga
     1621 ctcctgatac aggttgaagt agtcgcaaga taggtgtgtt tttcacattt ggagatgtta
     1681 taaaatggga atcatttaaa aaatgaaata agatgtttaa gaaaagatag acaagaggag
     1741 catatgacaa aggtagtaat aaagcaattg ttgcaggtaa tagtagtgtt catgataagt
     1801 ttatcgacaa tgacaggtgt tgtgtatgca gataaaggtc aaatttatgg atgtattatt
     1861 cagagaaatt atcgtcatcc gatatctggg cagatagaag atagcggagg tgagcattcc
     1921 tttgatattg gtcaaggtat ggttgaaggg actgtctata gtgatgccat gctagaagta
     1981 tctgatgctg gaaagatcgt tcttactttt cgtatgagct tagctgatta ttcgggcaat
     2041 tatcagttct ggatacaacc tggaggaact ggaagctttc aggcagttga ttacaatatc
     2101 acccaaaaag gtactgatac caatgggaca acactagata ttgctattag tttgcctact
     2161 gtaaatagta tcattcgagg aagcatgttt gttgaaccta tgggcagaga agtggtcttt
     2221 tacctgtcag cttcagagtt aatacaaaag tattcaggta atatgttggc ccagcttgtt
     2281 actgaaacag acaattctca aaatcaagaa gtaaaagaca atcaaaagcc tgttgataca
     2341 aaactaggag aaagtcaaga tgagtctcac acaggtgcta tgataactca gaataagcct
     2401 aaagccaact catcaaataa caagagtcta tcagataaaa aaattcttcc ttctaagatg
     2461 ggcttaacga cttctttaga gttaaaaaaa gaagataagt ttcggtctaa aaaagactta
     2521 tcaatcatga tttactactt tccaactttt tttctgatgt taggagggtt tgctgtatgg
     2581 gtttggaaaa agaggaaaaa aaatgataaa acgatgtaaa ggaattggtc tagccttaat
     2641 ggccttcttt ttggtagctt gtgtgaatca gcaccataaa acggctaaag agtctgaaca
     2701 gcagagaatt gtagccactt cggttgctgt ggttgacatc tgtgaccgtt taaatttaga
     2761 cctcgttggg gtttgtgata gtaaattata tacccttcct aaacgctatg atgctgttaa
     2821 gcgtgtgggt ttacccatga atcctgatat agagttgatt gcttctttga aaccaacttg
     2881 gattttgagt cccaattctt tacaagaaga tttggaaccc aagtatcaaa aattggatac
     2941 tgagtatggt tttttgaact tacggagtgt tgagggcatg taccagtcca ttgatgattt
     3001 agggaacctt ttccaacgtc aacaagaagc aaaagaattg cgccagcaat accaggacta
     3061 ttatcgtgct ttccaagcta aacgtaaggg gaagaaaaag cctaaagtgc ttattcttat
     3121 gggcttgcca ggtagttatt tggtggcgac gaaccaatct tatgtaggga atcttttgga
     3181 cttggcaggt ggtgagaatg tttatcagtc agatgagaaa gaatttctat cagctaatcc
     3241 tgaagacatg ctggctaagg agcctgactt gattttacga acagctcatg ccattccaga
     3301 caaggtaaaa gtgatgtttg acaaagaatt tgctgaaaat gatatttgga aacattttac
     3361 ggcagtcaag gaagggaaag tctatgattt ggacaatacc ctgtttggca tgagtgctaa
     3421 attgaactac ccagaagcct tggacacctt aacacagctt tttgaccacg tgggagatca
     3481 ttcgtaacat gacagcatca gatttacaaa gggatcaatc cctactggct aaaaaacgtt
     3541 tcagaagtgg tctttatcta gttcttttaa ttctcagtct cctgtttttg gcggttattg
     3601 ccttatccct tggtggtttg gcggtttctt atggggcaat tgtcaaggga ctgtttgtgg
     3661 cttatgaccc ccaagtggct ttgatttatg atttgcgctt tccaagaatt gtcattgctt
     3721 tattagcagg agcagggata gcggtttcgg gagttctttt tcaagcagtg ttgaaaaatc
     3781 ctatttcaga ccctgctatt atagggattt gtagtggtgc tagttttatg gttttagtct
     3841 ctagtttact tctaccgcag ttgctgctgt atggtccaat cgtatccttt ttaggaggcg
     3901 gagtgtcttt cctgctgatt tatggtttag catggaagaa ggggcttaat cctattcggc
     3961 ttattctaac agggattgct attaatgctt tatttatggg gttatcaact gctttaacca
     4021 gctttttcac ctcagctagt cccatggtca atgctctttt agcaggtcat attagtcaaa
     4081 aaacatgggc tgatgtaggg gttttattcc cttatacatt cattggcttg ttactagctt
     4141 tgttattgtc aaaaacctgt aaccttcttc ttttagatga tcaggtgatt cgtcatttag
     4201 gaatcgatgc tacagcgtta cggttgggaa tttccttggt tgctgtttta ttggcttcgg
     4261 tagccacttc gatcgttgga gtggtttctt tcctaggctt gattgtccct catatgagcc
     4321 gcttgctggt tggaagtaag caccaaatct taattccttt ttcagctttg cttggagcct
     4381 ttgtcttttt gctagccgat actttaggaa gaagtcttgc ttacccattg gaaattagcc
     4441 ctgcgattat aatgagtatt gtaggcgggc cttactttat ttacctgcta aggaggtctg
     4501 atattatatg agtaagaaca gcagtcatat tcaggtgcaa aacctttcct ttgcttatga
     4561 ggaaaaactt gttttagatg acctgacctt tagcattcca aaagggaaaa ttaccactat
     4621 tatgggggca aatggcagtg gcaagtcaac cttattacaa ttattaacca aaaaccttcc
     4681 gttaaaacaa gggcaggttt ggcttgagca ggaagaatta gcaacgattt ctctcaaggc
     4741 ttttgcgaaa aaagtggcag tggtacacca ataccatcaa gtggtcgaag gtttgttagt
     4801 gggtgaattg gtagccatgg ctaggcttgc ccgtcgttct ttttttaaaa ctactaccaa
     4861 agaagatgag gaacgccttg cttgggcctt agaagtaacg gctttgagtg attatgttta
     4921 tcgtgacctc caacaccttt ctggtggtga acagcagagg gtttggattg ccatggccct
     4981 tgcccaggat acagatatta tttttttaga tgagccaacg acttatttgg atattaagta
     5041 ccagcttgac attctcaagc tgattactaa aatcaaccaa gagctaggcc taacgattgt
     5101 gttagtatta catgacatca atcaggcctt gtctctttca gaccatctta ttggactgaa
     5161 agatgggcga ctttatgccc aagggcaacc aagagacctc cttgatcaac agtttatcag
     5221 agatgtcttt ggtgttgaat tgccctttgt tgagagaaat ggtcaacagt ttgtcctcac
     5281 ttcattggtc aatgaaacca atgatattag aactcaaagg agagatagga acaagaacag
     5341 atgatggaaa cagaagatac aatatcaagg ggaaaaagga aaagactgtt aaaacgatta
     5401 cgtgaacgaa ttgcaccaaa aaggtatttg ctttatgtgt cagccttttt atcttggtta
     5461 caatttgtga tgaggatgat cagtttttac ctcattgcca aaacgttttc gacctttatt
     5521 ttgggtcatg ctattgcctt aggacgcttg gcaggtttac tcctactttt aaatgtggta
     5581 ggctttgttc ttgctatatt aggcaagcaa ctgcaaggga tcgcttcaca atttgctaga
     5641 gattcactca aacaatcttt ctttgaggct tttattgatt tagacgggca atttgatgct
     5701 catgccagtg atgctgatat tttaacgttg gcttcacagg ggattgatag tttggatact
     5761 tattatggtt actatttgtc tttgtctatg agaaccaagt ggaactgcac aactattatg
     5821 atactagttt ttttaattta tccattggca ggccttgtct ttttaggagt tctgcttctg
     5881 attccacttt caatcgttgc tatgcaaaag cggtctaagc aaattatgag tcattattgg
     5941 tcttcttata tggatgttgg taatttattt atggatgact taaaaggtct taatacactg
     6001 tattcttatc aagcaataga acgttacgag caagaatttt cgggtaaggc agaacagttt
     6061 cgaaaggcta ccatgtcttt gcttggcttt cagttacaag cagttggtta tatggatgca
     6121 gttatgtatc tgggaattgg tttgtcaggt tttttagccg ttcaagcttt ggcaacaggc
     6181 cagttatcct tttttaattt cctctttttt ctcttgattg ctacagaatt ttttacaccg
     6241 attcgtgagc agggttatgg catgcacttg gtcatgatga ataccaaaat ggctgaccgt
     6301 atctttagtt tcttggatag tgttccagca aggaaagata acaagagtaa aacagcaata
     6361 aactttaacc agattgacat ccaaaatatt tctttagctt atgagaaaaa aacagtgctt
     6421 agtggggtta ccatgacatt aacgaagggc caactaacag ctatcgcggg ggtgtcagga
     6481 caaggaaaaa caagtctagc tcaactcctc ttgaaacgcc agtctgcaac gactgggcat
     6541 attttatttg acggtttaga cagtgacaat ttgttgcaag aaactatcaa ccagcaggtt
     6601 ctttatgtat cggaccaatc taccttattg aaccgtagca tttacgataa tttaaggtta
     6661 gcagcaaatc tctctaaaaa agaaatctta gattggatag atcaacatgg tttgttaagc
     6721 ttcatcaact ggctaccaga tggtcttgac acaatagttg gtgaaaatgg taacctgtta
     6781 tcaccaggcc aaaagcaaca ggtgatttgc gcaagagctc tattaagtaa gaggtctctt
     6841 tatatctttg atgaggcaac atctagtcta gatgcagaaa atgaacgtat tattgacaat
     6901 ttaataacga gattagctaa gacagcaata gttattgtga ttactcataa gatgtctcga
     6961 ctgaaaggag ctaaccaagt actcttttta aacacagggc agcctgcttg tttaggcaaa
     7021 ccttgcgacc tttatcggga ccaaccaact tatcgtcacc tagttgatac tcaagcaaga
     7081 ctggaggcaa gtatttatgg ctaaacctaa cagatctacc ttaagcctgg tttggcgttt
     7141 attaacccta atgaaaactc tgctaccttg gattatcttg gctgttggct ttgctgttat
     7201 gggttttgtc atcacggtaa gtattccaac tggaatagct tatttaggac tactagctat
     7261 tagacaagaa gtgattccaa tattagcttt atatcttttg attgctctag cctttttaag
     7321 aggttttgtt cgttatggag agcattattt tggtcatttt gttgcttttc atagtttagc
     7381 agcttttagg aacttgatct ttaagaaact aagagctctt tctccagctt gcttggatag
     7441 tcaagacagt ggttatttgc ttaaaatgat tggagaagat attgaggcat tagaagtttt
     7501 ttttgcccac acaatcgcac ctatttgtac agcaatattg tctgcaggtt taatgttctg
     7561 gtacttttgt caaagtagtt ggcagttagc cttattagct ttggcaacct atgcttgttt
     7621 agccattgtt atacccattt attttgctaa tattttacag gttttactaa aaagccaaaa
     7681 tgaaggtcga aaagattacc tgtcttattt tttagagagt ttacgatcag tcaaagatct
     7741 tttgcaattt caggtattag acgagcaatt tgaacgatta ataaaaaaaa gtaatcatgt
     7801 aaatgctctt gatcgtaacg tagcgcaagc ccaatttttg caaatggcat tgactttttt
     7861 ttggcttggc ttaatgatct tagcattttc ttatatggtt tttgatggga tatgccatga
     7921 cagcttatca tttgataaag gcttattaac ttttattgct ttcactgctt cgttttctcc
     7981 ttttttagaa ctaggacgtt tgccattagg ctttaagcgt gctatgaatg cggctcgaaa
     8041 tatttttgac cttctagacg aaaatgtcat agtagacgaa ggcacaaaac agatagatcg
     8101 tttaaggagt gtttcttttg aggacctcag ttttgcttat ccaaagcgcc aagagctggt
     8161 ttttaaggat ttatcagtta cctttcaaga aaaggggatt attggcatta aaggcgaatc
     8221 aggatctggt aaatcaactt tggtgaaatt aattatgaaa tggtataatt ggaagacagg
     8281 agatattttt cttaatgata ggaatagttg cttactaaat gctgctaaat tacaagatac
     8341 tattgcttat gtgccacaaa cagcgcagct tttccaacag tctatccgtg aaaatcttac
     8401 ctttggccgt caagatattt cagatgagtc catttggaat ttagctgaga cttgtggtat
     8461 gaaagacaga ctgttagctt gtaaggaagg tttggatacg attatcaaga gtccttctga
     8521 cttttcagct ggagaaggtc aacgtttaga gttgatgcga gctttattga aagatgcgag
     8581 ttgctatatt tttgatgaac ccacgtcaaa tttagattct ctaaatgaag ctatcttgct
     8641 tgatttaatt aaaacacatt gtcaaggatt ggtcttctta atttctcaca ggccatcaac
     8701 cctagcttgt gctgatcatc tattttgcgt aaaaaatggt tccctaaaag aggtgaataa
     8761 aaaatgaaac agttaacaat aaaagatatt atgataacag gcgcatttgc cgcgctctac
     8821 tttatttgtg ttggtctagg cacgtttatt aacatctttt ttgatcgctc aggaaatatg
     8881 ttatatgccc ctgcctttgc agctttactg gggggaacaa tttattttct tttgattgct
     8941 aaattgcaaa catttgggcc aataacatta ttaggattga tgatgggagg ttttttcttc
     9001 cttagtggtc acttttttgc cgcgatgttt cccagcatta ttttcggttt aatagcggat
     9061 attattgctt ctttaggaaa atacaagtca aggtttttta atgacctatc ttatatggtt
     9121 tttgcttttg tcaatagtgg ccctattgtg ctgatgtggt tgttgaaaga tgcctatgtt
     9181 gaaagtttgt tagcccgcgg taaaagtatg acctatgtca atcgcattat gtatgatttt
     9241 agtttagggc atgttatttg gtttgtagga attgttcttg caggagcctt attagggtca
     9301 gcttttgcac attatttact aaagaaacat tttatcaaat caggattgtt atcatgaggc
     9361 ttgatgttcg caccaaactg cttttgttgg tacttgccaa tgcctgcttc tttttccgtg
     9421 ttgatggctt tttagaattt ataattgtta tttttctctt attacttttg tctgctttaa
     9481 ataagaaaaa gctagcattc aagttggcgg ttgtttatct gttaatgatc ggtctatctg
     9541 taatcccgct atctattttc ccttcttatc ttgatcactt attgagtttt gtctcaatag
     9601 caggaaggtt agttttccct tctttactag caggtttaat tactattaag acaactacca
     9661 tctacgaatt ggttcatgga ttacgcaaat ggcatttccc agaagtctgg ctattgacct
     9721 tagccgttat gtgccgtttc ataccaatga ttagacaaga atgttgtgtt attcatcgct
     9781 ctttaacaat cagggggatt attttaacaa aatggtctat tttgatcaga cctaaacaat
     9841 acttggagta tttaatggtt ccattgcttt tatctttgat aagaagtagc caagaactaa
     9901 ctatcgctag tctaactaaa gggctagcag tcaacaaagg caccagtgaa tgctttagct
     9961 cacacttaac atggaaagat tggggggtgc agatttggat tactgttatc ataatcatca
    10021 cgatactgca atgatttcag cagaacaact tgtttttacc tatcatgatc aaaaaaatcc
    10081 agcttgtcag gtatcgactt gtcaaattgc ctctggacaa ttcattgttt tgtgtggacc
    10141 ttctggttca ggtaaatcta cctttttaaa acttcttaat ggcataattc ctgattatta
    10201 tgctggaaaa tatgaaggta gactagatgt ggctgattgc caagcagaca gagacagtgt
    10261 tgaaacattt tctagatctg tggcctctgt ttttcaaaat ccagccagtc agtttttcta
    10321 tcgcgaagtc caacacgaac tggtctttcc ctgtgaaaat caaggtttag atgcaaaggt
    10381 tatcatgaag cgtctttgga ctttagcaga agattttgct tttgcagagc tccttaacaa
    10441 ggatatgttt ggattgtcag gagggcaaaa acaaagagtt gctattgcaa cggccattat
    10501 gcaaggtact aatattatgc tttttgatga gccaactgct aacttagata gtgcaggcat
    10561 agctgctgtt aaagcctatc ttacccaatt aaaagcagca ggaaaaacca ttattgtagc
    10621 cgagcatcgc ttacattact tgatggattt ggcagataac tttttctact ttaaaaatgg
    10681 tagattaaca gataagttaa caacacaaga tttgctagcc ttgacagatg aacaacggca
    10741 agatatggga ctacgtcgct tagacttgtc tgacctaaaa cctgtgttag ctggtaagat
    10801 tgagagtcaa cattatcgcc cagatgatag cctgtgtatt gagcatctaa cagttagagc
    10861 gggaagcaaa atacttagat gtattgaaca gcttagtttt gctgcaggaa gtatttctgg
    10921 cattacagga tcaaacgggc ttggaaaatc gcaattagtt tattatatag ctggtatctt
    10981 agacgataaa aaagcaacta ttaagtttca aggcatacct ttatctgcca agcaacgttt
    11041 atcaaagact agtattgtct tacaagaagt ttctttgcaa ctatttgcag aatcggtttc
    11101 aaaggaagtc aacttgggcc atgaacgaca tccacggacg acagaagtaa tagagcggtt
    11161 atctttaacg actcttttag aacgtcatcc agctagtttg tcaggaggtg agcaacaaag
    11221 agtgatgatt gccgctagtc ttttggctga taaagatatc ttaatttttg atgagccaag
    11281 tagcggactt gatttgttac aaatgaaggc tttagctaat cttctcatgc agctgaagac
    11341 ccaacataag gttgtcatcc tcatttcaca tgatgaagaa cttctaagta gtgtctgcga
    11401 taccatttat caaatgctac cttaacgagc ccctagaact gtttaaaagt actaaaaggc
    11461 ttttatctga aaataaatag gaactcttgt ataatagata caagagtcag aacgtagaca
    11521 actccagttt tatgctcaaa aagcatgcat agctctgctg atttgacatt cggtttccga
    11581 tttttacata taaagtgagc caaaacagat gctttttgct gtggtaaaag agtggcgtca
    11641 atacgagatt gacgccactc gggatttttg agacctcagg ctcaaaaata aaggcatgga
    11701 attccaaaga acgtcgctgc cgtaagcacc acctaaggaa agtatcacga caaactttgt
    11761 cttcaatctg acaagagttt ttgtgaaggg aaaaagataa tgatattaac agctcctatg
    11821 tccaacttaa agggatttgg accaaaatca gcagaaaaat ttcagaaatt agatatttat
    11881 acagtagaag atttactgct ttattatccg tttcgctatg aagattttaa atcaaaatct
    11941 gtttttgatt tagtggatgg tgaaaaagca gtcattacag gcttagtcgt tactccagct
    12001 aatgtacaat attatggttt taaacgtaac cgtttaagtt tcaaattgcg tcaaggggaa
    12061 gctgtcttaa atgttagttt ttttaatcaa ccctatttag ctgataaaat agaacttggt
    12121 caagaggtag ctgtttttgg taaatgggat gccactaaat cggctattac tgggatgaag
    12181 gttttagctc aagttgaaga tgacatgcaa cctgtttatc gcgtagctca gggaatttca
    12241 cagtctactt tgattaaagc tattaagtca gcttttgaaa tcgatgcgca tttggaattg
    12301 aaggaaaatt taccagctac tttattggaa aaataccgat tgatgggtcg tagtcaggct
    12361 tgtttagcta tgcatttccc aaaagatatc acagagtata agcaagcgct ccgtcggatt
    12421 aaatttgaag aattatttta ctttcaaatg aaccttcaag ttttgaaagc cgaaaataga
    12481 tctgaaacaa atggtttgcc tattctttat agtaaacatg ctatggagac aaagatttcc
    12541 tctttaccct ttattctaac aaatgctcaa aagcgctctt tagatgacat attatctgat
    12601 atgtcatcgg gagctcatat gaatcgttta ttgcaaggag atgtaggatc aggaaagaca
    12661 gtcattgctg gtctatcaat gtatgcagct tatacagcag gttttcaatc ggctttgatg
    12721 gttccaacgg aaatcctagc tgaacaacac tacattagtc tgcaagagtt atttccagat
    12781 ttatcaatcg ctatattaac ttcgggtatg aaagcagctg tcaagcgtac ggttttagca
    12841 gctattgcaa atggctcggt tgatatgatt gtaggaactc atgctcttat ccaagactcg
    12901 gtacagtacc ataaactggg gcttgtcatt acagacgagc aacatcgttt tggtgttaaa
    12961 cagcgtagaa ttttccgtga aaagggagaa aatcctgatg ttttaatgat gacagccacc
    13021 ccaattcccc gaactctagc aatcacagct tttggagaaa tggatgtttc tattattgat
    13081 gaattacctg ccggtcgtaa acctattatg acacgctggg tgaaacacga gcagctaggt
    13141 actgtgttgg aatgggtaaa aggtgaattg caaaaagatg ctcaagtgta tgtcatttca
    13201 ccgttgattg aagaatcaga agctttagat ttaaagaatg cagtagcatt gcatgctgaa
    13261 ttatctactt attttgaagg aattgctaag gttgctcttg tacatggacg tatgaaaaat
    13321 gatgaaaaag atgctataat gcaagatttc aaggataaaa aaagtcatat tttagtatcc
    13381 acaacagtta ttgaagtagg ggtaaatgtc ccaaatgcaa cgatcatgat tattatggat
    13441 gccgatcgtt ttggattaag tcagttacat caacttcgtg ggcgtgttgg tcgtggatat
    13501 aaacaatcat acgctgtttt agtggctaat cccaaaactg attcggggaa aaaacgaatg
    13561 acaatcatga cagaaacgac agatggtttc gttttagctg agtcggattt aaaaatgcgt
    13621 ggttctggtg aaatctttgg tactcgtcag tctggaattc cagaatttca agtagctgat
    13681 atcgttgagg attatcctat tttagaagaa gcacgcaaag tttctgcagc gattgtttct
    13741 gatcctaact ggatatatga aaaacagtgg caattagtgg cacaaaatat tagaaaaaaa
    13801 gaagtttatg attaacttaa aacaagtcgt tgttttacaa atatttagtt agttttgaga
    13861 taaactgtta taaagtataa agaggtcact atttctggcc ttttttactt atgctttacg
    13921 atgagactgc tatgtgaagc agtattaacc cattttcagt ataacttttt tattgctaaa
    13981 ataatcatat tattaaaatt cttatttata tttttttaat aaaaagaaga acttgtcaca
    14041 accgtttaca gatgttatta tgtgtaagat attgtattaa tcgataagta caaaaaagga
    14101 gcggaaaatg acacagcaat caatgttact aagtattgag caagtaaata aatcctatgg
    14161 taaaaatcag gttttaagtg atatctcttt tgatatttac aaaggagaaa tttgcggcct
    14221 tgtgggtcaa aatggtgctg gaaagacaac gttaatgcgt attttatctg gcctaattgg
    14281 aaaagattca gggcaaatca aacagttaca accctatcga atgggatcaa ttattgagtc
    14341 accgacactt tatcccaata tgacagctca tgataatctt tactatgcag cgttgcaatt
    14401 gagattagct gatgcaaaag agcgcataca tgaggttttg gaacttattg ggttagaaaa
    14461 agtgagcaaa aagaaaaagg tgaaagatta ttctttaggg atgcgccaac gtttggctat
    14521 tggtctttcg atactggatt ttccagaatt tttaatcttg gatgaaccca ttaatggtct
    14581 tgatccagca ggaatcaaag aaatgcgtca gattattttg aacctaagag attgctatgg
    14641 tattacgatt ttgatttcca gtcatatttt atcagaatta gacttagttg ttgatcgtta
    14701 cgtgattatg cataaaggca aagttattaa gagtatggac aaagcagagc ttaaagctca
    14761 agtaaaagta caaattgccc ttcatacaag cgatgatcag ctggtaaaaa atagactttt
    14821 agaattgggg cttagagttg agactgatgg tcagatgcta ctaataaaac caactttatc
    14881 agtaatggag cttatcaaaa ttgttctaga tctgcctgtt gagatttttg acatttatca
    14941 tcatcaagtg agctttgaac attattacct cgatttgtta ggtaaagatg cagcatcacc
    15001 attaatttag aaagaggttt ttatgatttc ctatcttaaa gcagatttat ttcggatacg
    15061 ccaagagcgt ttagctctat tttcttgtat gctacttgtt gtcattgctg ttggtgtagc
    15121 ctatagtttt cgcaagacac ctaaccaagt catcatgata caaatcatta atattattac
    15181 tgattttgtg acgcttttct ttttaacacc agcccgtatc ttttttagtg atgactataa
    15241 ctaccgtacg attaataatg ttgtggtcaa acaacaaaat cgtactttaa ttataatcta
    15301 taaactgcta gttagttttg gcttatctct tttatttatt agtttaactt atcttcttag
    15361 tgctttagca tttcatttat ttactggtca agcatactat ggaatagtca tagatcggat
    15421 gattaaacaa ctgccttttt atattgctat tattgctttg tgtcaatttt tgtttaatat
    15481 acttcgtaaa acttatcagt caatcacagc ttatatactt tatgtccttc tttttgataa
    15541 agtatctgct actttagcaa accatttttt taagttagat attagcccag ttttgatgtt
    15601 taaacaccta tccttgtctc ttggtgataa acacctttct agtactagca tattagtggc
    15661 ctttggttta actctttgtt atcttttagg caatgtcttt cttttttcca aacgagaact
    15721 aaaataaagg gtaatatgtg ctttgtttat tttaaaagac aaagaagttc ttagaaaaag
    15781 gtcgtcttca tcattactaa aaaaggataa gcaagtagtt ttgttattat tcaagtaagc
    15841 tcatacttaa tgagacaaaa taaagacttg tttacccggt atctgattgc taagagttgg
    15901 atttattgga atactggtct caaactgtta tagaaatgat tatgagtgca caatctcata
    15961 gtttgaaaca tagttgttta ttctagacat ttaggagatg tcctaattta aagcttaaag
    16021 tactagaaat gtcccactaa tatgttttag tgggacattt ctaaaggaag ctagattatc
    16081 cttcaatata atcttttagc tcttgaccag taagtccggc gttgagagca attaataact
    16141 ttagacgggc ttttggagca ttcaattctt ttacaaacat aacacctgct tcttgtagca
    16201 tcgcaccacc accttcataa gcatagacag gttctgccat accattgaaa caacgggaaa
    16261 caagaacgac aggaatacct aaagtaatta agtgctcgat ctccccaact gctaatggtg
    16321 ggacatttcc tgcccctaga gcttcgataa ctagaccttg aatgctacct ggagttaata
    16381 gactcaagat actaccatcg cccatacctg cgtatgcttt gataatgggg atggttccag
    16441 aaatgcatct caagtcaaaa cgaatacgag gttcagcagt tttgaaaaaa agtaggtcat
    16501 ttttcatgat gataccaagc ggtccgtgtg tgggtgtttg gaaagttgaa atattggttg
    16561 tatgtgtttt agtaacatat ttagcagcat ggatttcatc gttcataaca acaagtaccc
    16621 ctttttcttt agctttatca ctacttgcaa ctctaagtgc ggtaaggtaa ttatatatgc
    16681 catcactacc aacttcatta gaagacctca tagcacctgt taggacaaca ggtatattag
    16741 taggcaatgc catggtatct aaaaaataag ctgtttcttc taaggtatct gttccatgtg
    16801 tgatgactat accatcaaag acatcagttc tttcttgaat atgatgataa atagataaca
    16861 tgtggtgagg cgtgatgtga ggactcggaa gatttagaaa atcactaacg gtaagctgaa
    16921 tatcttgggc tgcagcatgt attttcgtca taggattatc ttgatttgga acaaccctac
    16981 cagagttatc agcttgcata gaaatggttc caccagtgtg taagactagg atttttttca
    17041 tttgattgtc aaacttcctt ttatttatga tataatctat tctatcataa acacaggagg
    17101 caaaagaagg ttttgacagt aaaagcagtc ttttttgata ttgatgggac ccttctcaat
    17161 gatcgtaaaa atatccaaaa aacaacgcaa aaagccatcc aacaactcaa aaaacaaggc
    17221 attatggtcg gtttagcaac tggtcgtgga ccgggatttg tacaaccttt tctagaaaat
    17281 tttggattag attttgccgt tacttacaat gggcaatata tcttaacaag agataaggtc
    17341 ttatatcaga atcaattgcc aaagtcgatg atttacaaag tgattcgcta tgccaacgaa
    17401 aaaaagcgag aaatttctct tggaacagct tctggattag caggatcgcg tattattgat
    17461 atggggacta gcccatttgg acaagtcatt agtagttttg tgcccaaaag ttgggcaaga
    17521 acagttgagg gtagttttaa gcatttgatt cgccgaatca agcctcagag ttttagaaat
    17581 ttagtaacta ttatgcggga gcctatttat caggttgttc tggtagcgtc acaagctgaa
    17641 actaagaaaa tacaagagaa gttccctcat ataaaaatta caagaagtag tccttattcc
    17701 ttggatctga tctctgttga tcaatcaaaa ataaaaggaa ttgaacgttt gggagagatg
    17761 tttggatttg acttgtctga agtgatggct tttggagatt cagataatga ccttgaaatg
    17821 ttatcaggag ttggtatcgg tattgctatg ggaaatgctg aaactgtggt aaaagatgga
    17881 gcccatttca ctaccgactc taataataat gatgggattt ccaaagcctt agctcactac
    17941 ggtctcatcc attttgatat agagaagagc tttaaaagcc gtgatgaaaa ttttaataaa
    18001 gtcaaagatt ttcatcgtct gatggactca gatactattg aaacaccgcg tagttatacc
    18061 atttcagaag ctggttatag gtcaggcttt aaagtagaag aaattgttga gtttctttac
    18121 gcagcaagca agggagacca acaacaattt acacaggcta ttttcgattt gcatggtgct
    18181 gttgaccaag cagctaataa ggtacaagct aaaaaacatg ttgaaacgcc cctgattggg
    18241 caagttgatg ccttagcgga tttgctttat tttacttatg gttcttttgt actgatggga
    18301 gtagatccac aacctatatt tgaagcagta catgaagcaa atatggctaa aatttttcca
    18361 gatggcaagg cacactttga ccccgttacc cataaaattc aaaaacctga ctattggcaa
    18421 gagcgtcacg cacctgaagt agccataaaa aaagagttag acaaacaatt acaaaaatca
    18481 ctacaacgat tcgataaata gaggtttact cttttaaata tcaagaaata aaaagatatt
    18541 gactagactt agctcagcac aaaaaaacaa gcaatttcgt tgtagatgct gcttgttttt
    18601 tggtgtttga aaattgtaat agactagagt gttttagtgc tatctcggac tacaagtaag
    18661 tcaatcttag cgtgtcgcat aatgtactca gaagatgagc caataaggag gcgttcaaag
    18721 gtattaagac cagtagctcc taccataatt aaatcagcgt tctcacgatc tggaatatcg
    18781 tgagctagta agtttttggg gttgccaaat tcaataattt gtttgatgtt tgtaattcct
    18841 gctatctgtg cttgtttttc aaaatcatct aaaacatcct tagcttcttg ttctaatttt
    18901 tcatagatgt aagtatcaaa agtagctacg ctttgcaagg cacgggtatc aataacgtgt
    18961 actagtagta aggtagcgtc gttacgtagt gctacgttaa cgcctttatt gaaagctaat
    19021 tcagactcat aagagccatc aatagcaacg aggatacgtt tatattttag tgacatagaa
    19081 ctgtcctcct ttaacagttt gacaagaata gcttttattc tttttttcat tatactcttt
    19141 tttgagtcat tttgaaagcg atccattatt tttgctaaac taaagactgt attatcgtct
    19201 cataagacct gtgttaaaac gattagctgt cctaagcttt gatggataaa ttctttctat
    19261 tttaagtatt ttggtttata atatggaata cgaggtgtat gagactatga aaattattga
    19321 aaaatcatca aaattagaac atgttgccta tgatattcga gggcctgttt tggatgaagc
    19381 taatcgcatg attgcgagtg gtgaaaaaat tctgcgttta aatactggca atcctgctgc
    19441 ttttggtttt gaagcaccag atgaagtgat tcgtgattta attgtcaacg cgaggctaag
    19501 tgagggctat tctgacagta aagggatttt ttcagcgcgt aaggctatca tgcagtattg
    19561 ccaactcaag ggcttcccag atgttgacat tgaagatatt tatttgggaa atggcgtgtc
    19621 agagttgata tccatatccc ttcaagcttt gctagataat ggcgatgaag ttttagtacc
    19681 aatgccggat tatcctttat ggacggcttg tgtgagtctt ggtggaggaa aagcggtcca
    19741 ttatctttgt gatgaagaag ctggttggta tcctgatatt gctgatatca aatcaaaaat
    19801 cacgagtcgc accaaagcta ttgtggtcat caatcctaat aacccaacag gagcccttta
    19861 tcctaaggag attttagaag acatcgttgc tttggcaaga gaacaccagc tgattatttt
    19921 tgccgatgaa atctatgatc gtttggtgat ggatggtaaa gagcatattg ctattgcaag
    19981 tttggcacct gatgtttttt gtgtatctat gaatggtctt tccaaatctc atcgtattgc
    20041 tggttttcgt gttggatgga tggtcttatc aggtcctaaa gaccatgtta aaggttacat
    20101 tgaagggtta aacatgcttg ccaatatgcg gttatgctcc aatgttttag cccaacaagt
    20161 tgttcaaact tccttaggag gtcatcaatc agttgatgca ctgttactgc caggagggcg
    20221 tatttttgag caacgtaatt ttatttatga agctattaat gctatccctg gcttatcggc
    20281 agttaaacct gaagcaggtc tttatttatt tccgaaaatt gatcgtcaga tgtatcgtat
    20341 tgatgatgat gaagaatttg tcttacagtt attaaagcaa gaaaaagtga tgttggtaca
    20401 tggacgtggt tttaactgga aagatccaga tcacttccgt attgtttacc taccaagtgt
    20461 agaagaatta gaagatgtcc aagaaaagat tacacgtgtc ttataccagt atagacgtta
    20521 atgcaatgct catctttgat taataaaaat agcgtttctt aaaagaatgt gatcctatgc
    20581 tctaacaagc tagtgcttat ctcctttaaa tgaagtgata ataaggcatc aatagttgga
    20641 attgtagtaa gaatagaaaa ttttctgata attccttgca aaagcgagtt agctttgtta
    20701 taatgaaagc gcaaataatt caaaggaaaa ggaaaaaaat gcctaactta ttagaaaaaa
    20761 ctcgtaaaat cacatctatt ttgcagcgtt ccgtagatag cctagaaaca gaattaccgt
    20821 ataacacaat ggcatctcgc ctagcagata tcattgactg caatgcttgt attatcaatg
    20881 gcggcggcac actgcttggt tatgccatga aatataaaac taacactgat cgtgttgagg
    20941 aattttttga agctaaacaa tttccagata cttatgtaaa ggctgctagc cgagtttacg
    21001 atacagaagc taacctttct gtcgaaaatg aattgactat attccctgtt gagtctaaag
    21061 acacttatcc aggaggtcta acgactattg cgccgattta tggtggaggg atgcgccttg
    21121 gatcactcat tatctggcgt aatgacaatg agtttagtga tgatgatttg attttggttg
    21181 agatctcaag tactgttgtg gggattcaac tattaaatct tcagacagaa aacttagaag
    21241 acaccatccg taaacaaaca gcggtcaaca tggcaattaa tacgctttct tattcagaaa
    21301 tgaaagctgt tgcagcaatt cttggagagt tagatggtaa cgaaggacga ttgacagctt
    21361 ctattattgc tgatcgtatt ggtattaccc gttctgttat tgtcaatgct ctgcgtaaat
    21421 tagaaagtgc agggattatt gaaagtcgtt ctcttggtat gaaagggaca tacctcaaag
    21481 ttatcaacga aggtattttt gctaaattaa aagaatttta aatagaaaaa gacaacatct
    21541 cttgaaaaga gatgtttttt gctgttaatg gtagatgcaa atagatgtct tgatacctgt
    21601 aataaaaact gatacaatgc catatataag agataagggg agaggtttcc ctgtttagga
    21661 gagagtgatg agagcattga tttcaattga ttacacaaat gattttgtag cagatgatgg
    21721 taaattaagt gcaggtaaat ctgctcaggc tattgccact aaaatagcag aggtaactaa
    21781 aacggcattt gatcaaggag actatatttt ttttgctatt gattgtcatg atcaaaatga
    21841 tccttggcat cctgagagta agttatttgc agcacataat atcaaaggaa ctactggacg
    21901 ccatctttat ggtccactag ctgaagttta cagttatatg aagcaacatc ccagagtttt
    21961 ttggattgat aagcgttatt attcagcttt ttcggggact gatttagata ttagactacg
    22021 agaaagaggt atcactcaat tagtgttaac aggagtttta tctgatatct gtgtcttaca
    22081 tactgctatt gacgcttatc atcttggtta tcaattagag attgttaaat cggctgtcgc
    22141 tagccttaca aaagaatctt atgagtggtc tttggctcac tttgagcagg ttcttggtgc
    22201 taagcttatt aacgattact aaaagagcct accaaactat tgattcagtg actaagagtt
    22261 tggtaaccgt aatcattgct ctatgtggtt ttaccgtatg tatgggttag gtagatgagg
    22321 tatgtgacat gctggtcaga attagtcctg atgattcttg tgctgtctaa aaggcaacta
    22381 aactttggac tgtgtcagat agaacacatg tttttttgaa ggagacagga ttttagctgt
    22441 ttgagaaatg atttgagttg gtggtttaga ggatcgtctc acagccatta gacaaactct
    22501 agtcttttca gcattagaat ataaataaag cttcaaactc ttgaatgcta gagtctgtac
    22561 catttttatc ttaggcttat cacatcacat gttgtcacaa tcgttgagga tttggtaaat
    22621 tagaataaag caccccttta ctaccttcgt aaacatatta ccgacttgaa agaacgttac
    22681 tttttctgtg atgttgtagg aaaatacttg gaagaatacg ttgacagggg tatttttgat
    22741 aaggagccgt tccagacctt tgatcagaaa gggattggcc gtctctttag cccgttcggt
    22801 taagcctgag ttgaaacttg gtatttgtgg ggaacatggt ggcgatcctg cttccattga
    22861 cttttaccac agccaaggcc tgacctacgt ttcttgttcg ccatttagag tgccgcttac
    22921 tcgcttggcg gctgctcagg ctgccatcaa agcttcaggc cacagtctta cccaagacaa
    22981 atagagttta gtttgtcaca cttcctgact aaggttttct gaccttagtc aggaagtttt
    23041 ttgctgtata gctttctaga attgcattag tcacctatgg taaatgggcg caattgtgat
    23101 ataataattg agactatgta aaaacgatgt gaggagtttt tccgctacct ttggagaata
    23161 gcagagctgt tctttggaat tggaatactt ctgatcatct tggatgggag aaatcatgaa
    23221 aatttctgaa gaagaagttc gccatgtcgc gaagctatca aaattatcat tcttagaaag
    23281 tgagacaaca acctttgcaa cgaccttatc caaaatcgtt gatatggttg aattgctgaa
    23341 cgaagttgac accgaaggtg tcgccattac aacaaccatg gcagataaga aaaatgtgat
    23401 gcgtcaagat attgctgagg aaggaacaga ccgtgccttg ctctttaaaa atgttcctga
    23461 aaaagaaaat cactttatca aggtacctgc tattttggat gacggaggag atgcctaatg
    23521 tcttttaatc ataaaaccat tgaagagttg catgacctcc ttgtagctaa ggagatttcc
    23581 gcaaccgaat tgactcaaaa aacacttgaa gacatcaagt ctcgtgagga ggctgtcggg
    23641 tcgtttatca ctgtttcaga agaagctgct cttaaacaag ctgcggcaat tgatgccaaa
    23701 ggtattgatg ctgataacct catgtcgggt attccactag ctgttaagga taatatctca
    23761 accaagggca tcctaaccac agccgcttca aaaatgttgt ataactacga gccgattttt
    23821 gatgccacat cagtagcaaa tgcctacgct aaagacatga ttgtcattgg aaaaaccaac
    23881 atggacgaat ttgccatggg tggttcaaca gaaacctctt atttcaagaa aacgaaaaat
    23941 gcttgggacc acacaaaagt acctggtggc tcatcaggtg gttcggcaac agcagtcgct
    24001 tcaggacaag tccgtttatc tcttggttca gacactggtg gttctattcg gcagccagct
    24061 gcatttaatg gtgttgtcgg tttgaaacca acctatggca ctgtttctcg ctatggtctc
    24121 attgcttttg gaagttcact tgatcagatt ggaccttttg ctccgacagt taaagaaaac
    24181 gcccaattat taaatgtgat tgcaagtagc gatgtcaagg atgccacttc agcgcctgtc
    24241 tgcattgcag actacacatc taagattggt cgtgacatta aaggcatgaa gattgccctt
    24301 cctaaagagt atcttggcga agggattgat cctgagatta aggaaacggt tcttgcggca
    24361 gctaaacaat ttgaagcctt aggagctact gttgaggaag ttagtcttcc acatagcaaa
    24421 tatggtgtgg ctgtctatta cattattgct tcgtcagaag cgtcctcaaa cttacaacgt
    24481 tttgatggca ttcgctatgg tttccgtgct gacgatgcta aaaacttgga tgaaatctat
    24541 gttaacactc gtagccaagg ctttggtgat gaggttaaac gtcgtatcat gcttgggacc
    24601 tttagtttgt catcaggtta ttatgacgct tatttcaaaa aggccggtca agtgcgtacc
    24661 ttgattattg aagattttga caaggtcttt gcggattatg atcttatttt aggcccaaca
    24721 acaccgacgg ttgcttttgg cttagacaca ctcaaccacg atcctgttgc catgtatttg
    24781 gccgacttat tgaccattcc agttaacttg gctggtctgc caggaatttc cattccagca
    24841 ggattcgtcg atggcttgcc agtagggctt caattaattg gtccgaaata cgctgaagaa
    24901 accatctatc aggcggcagc agcctttgaa gcggtgacag attatcacaa gcaacaaccg
    24961 attatttttg gaggtgacaa gtaatgaatt ttgaaaccat tattgggctt gaagtccatg
    25021 tggaattaaa taccaattcc aaaattttct caccttcgtc agctcacttt ggtgaggacc
    25081 caaatgccaa cacgaatgtg attgattggt ctttcccagg ggtcttacca gtcatgaaca
    25141 aaggggtgat tgatgcgggc ataaaagctg cgcttgccct caatatggac atccataaag
    25201 agatgcattt cgaccgcaaa aattatttct acccagacaa tcctaaagct taccaaattt
    25261 cacagtttga cgagccgatt ggctacaacg gatggattga gattaaattg gaagacggct
    25321 cgactaagaa aattcgtatt gaacgcgcac acttggaaga agatgctggt aaaaacactc
    25381 atggcacaga tggttattct tatgtggacc ttaaccgcca aggagttcct ttgattgaga
    25441 ttgtgtcaga agccgacatg cgttctcctg aagaagccta tgcttatttg acagctctga
    25501 aagaaatcat ccagtatacg ggtatttctg atgtcaagat ggaagaaggc tccatgcgtg
    25561 tggatgccaa tatttctctt cgtccttacg ggcaagaaca atttggaaca aagaccgaat
    25621 tgaaaaactt gaactccttc tcaaatgtcc gtaaagggct tgaatttgag gtggaacgtc
    25681 aagccaaact cttgcgttca ggtggtgtga tacgccaaga aacgcgccgc tatgacgaag
    25741 cgaataaggg gactattttg atgcgggtca aagaaggggc agcggactat cgttatttcc
    25801 cagaaccaga cctgccatta tatgagattg atgatgcttg gattgacgag atgcgtgctc
    25861 aattacctca attcccagct cagcgtcgtg ctaaatacga agaggaactt ggcctctcag
    25921 cttatgatgc tagccaacta acagctacca aagccttgtc agatttcttt gaaactgcag
    25981 taagtcttgg tggcgatgcc aagcaagtct ctaactggtt gcaaggggaa gttgcccaat
    26041 tcttaaatgc agaaggcaaa accattgaag aaattagatt gacgcctgac aacttagtgg
    26101 aaatgattgc tattattgct gatggtacaa tctcttctaa aatggctaag aaggtctttg
    26161 tgcatttggc taaaaacggt ggctcagctc gcgcctacgt cgaaaaggca ggacttgttc
    26221 agatttctga tcccgctgtt ttggtaccaa ttattcacca agtctttgct gataatgaag
    26281 ctgcagtggc tgatttcaaa tcaggcaaac gtaatgctga taaggctttc acaggcttct
    26341 tgatgaaggc aaccaaaggt caagctaatc cacaagtcgc tcaacaatta ttggcacaag
    26401 aattgcaaaa attgcgagat tgatagttgc tgatagcaat aagttaaagt attttgtctc
    26461 tgaaaaaaag gcggtcggtc tttcacaggc tgcctttttg tatgtcatgt tagtgaccgt
    26521 ctataaaaag accatcatct atgctaaaat aagactgaca ggtattgctt tagaaccttc
    26581 aggagtttct gacgtgtatt taatgaacta tcacgtttgt ctacactatt attagcgatt
    26641 agctgtgtga taaagtttgt taagttattt agtgtgtgaa gacttctagt cgttctcggt
    26701 ctatctacct taatagaagg aattgttttt tctattttac tcaggcacgg aaacatttac
    26761 taaagaagaa ggtaaaggaa tcaactaaag ttaagaacat ttaatggata gcagttcttc
    26821 taaaaggatt ttacaagaaa ggaggtgtta acgatgatat ggtctttaat tgtaggtgcc
    26881 tttatcggca tgatagcagg taaaatgact gaaaaaagcc agtccatggg ttgttttact
    26941 aatattatcg caggtctcat agggtcagct gttgggcaaa ggctttttgg ggattgggga
    27001 attcagttgg caggtatggc tattttcccg tccattttgg gagcggcaat tgtaattgta
    27061 cttgtttcag ctatttttgg aaaaaggtat taaaaaaagg ctgcatggaa gtttccaagc
    27121 agcctttttt gcaacataaa atgtcttatc tttttattgg ttgcatcctc attataagat
    27181 agttgtgtga aaatttaata tcattttctt gacaaatagt tagattttgt tactgttttg
    27241 acatcatttt gtaatgtcag tttagttgta gtgtgggtct gttgttatat atatttgagc
    27301 gaaacgcaca aatgtaatcg tttggaaatt tatgtgatga tttgaattgt agcaatagca
    27361 ttatttttaa taaaatagac aataagtcag tggtctattt gatctcaaat agctatcagg
    27421 aagtgttttg caagtgtttt ctttaaatgc tataataatt aataagacag tttaggaggt
    27481 tgaaatgaca aaaacaaggt tgtatattgc cagacatgga aaaacgatgt ttaataccat
    27541 tggtcgcgct cagggttgga gtgacacacc tcttacaaaa aaaggagaag aaggtattcg
    27601 agaacttggc ttaggtttaa aagatgcaac tataccattt aaggctgcgt tttcaagtga
    27661 ttcaggtcga accatgcaga caatagaaat tattttaaga gaatcagaaa acgaattttt
    27721 gccttatact aaagataatc gtattcgtga gtggtgtttt gggagtctag aagggactta
    27781 tgattcagaa ttatttctag gtgtccttcc aagaacaaaa gcctttgaaa atcgtgataa
    27841 cctacgtgat gttccctatt ctgaactggc agaaagcatc gtggaagtag ataccgctaa
    27901 ttgggcagaa ccatgggaag ttcttcgtaa gcgcatttgg gaggggtttg aagctattgc
    27961 tttgtctatt caaaatgctg gcggcggaaa tgctcttgtg gttagccatg ggatgactat
    28021 tggaacattt atgtggctaa tcgatcctga ccgtgagaaa caatatattg ataacggcag
    28081 tgtcactgtt gtcgaatttg aagatgggca atttaccatc aaaaccattg gagatatgag
    28141 ttatcgctac cgtggccgtg aaataataga agcaatgtca gatgaagcat aatcatttta
    28201 aattattatt gcaaacactg gttgttctgc tctttgtcgg aggtatttac ctctttatta
    28261 agccagagaa gcaggccacc aagacgcctt caagtcaaca ggcaagcagt caaaaagtgg
    28321 tcaaaaatag caaagagtcc acagggcttc ctaaagtctc tccaaaagat tgggaattga
    28381 ttttagtgaa tcgggaccat atgactgagg aattgagccc agaattagtt gacattaatg
    28441 gtatttctgt ggataaacgc attgagcaag ccactagtga ttttttagcg gcagctcagg
    28501 ctattgaccc acaagagcac cttatttcag gctatcgctc cgtagcttac caagcagagc
    28561 tttaccaatc ttatattaaa aaagagatgg ctaacgatcc aacattgaca caagaagctg
    28621 cggaggcttt ggtgcaaacc tatttacagc cgccaggggc tagtgaacat cacactggtc
    28681 ttgctattga catgagcact gtagatactt taaatgccag tgacccatcg gtatctaaag
    28741 cagttcaaaa aattgcacct gattatgggt ttgtcttgcg ctttccagaa ggaaaaaaga
    28801 ctagtacagg ggttgattat gaggattggc attaccgcta tgtaggcaag gcgtctgctc
    28861 gttatatggc tcagcacaac ctaacgttgg aagagtacat tgctgcttta aaggagaaac
    28921 gatgagaaaa cgactaaagt ttccgtattt tttaacgctt ctagcttgct ttttattgct
    28981 gattgtttgt cccttactga gtagccaaag gatagctagt gcagataaag aagtaagggt
    29041 gaactacagc caaaaacaat ttattacaaa aatgggtaaa gaagttaaac cattggcaaa
    29101 atattatggc attagaccat ctattttgat tgctcaaatt cttttggaaa cccatgatgg
    29161 aaaaacatta ctagcgtcta agtatcataa tctttttagc aagaaagcaa ctccaggaca
    29221 agcggccatt accctaaagt cccctaaaca aaccaaccaa aacgtgagat atgctattta
    29281 taaagatgac gctagtgcaa ttagagatta tttacgaatg cttcggcagg gaaaagaagt
    29341 cgataagcgt ttgtatcgta atcttgctac agaaaaaggg tataaagcac cagctaaaag
    29401 tttacaaaag tatttgcatt atactgataa aacctatgct aggcgactga ttcaggtcat
    29461 tgaaagtaac gatttaacaa actatgactg aaattagcac tctttataaa agagtgctaa
    29521 tttttttgag tttttttctt gacatgtgtc ccataaagag tataatagaa ttataaatta
    29581 gcagtctact aaatagagtg ctaaacgaaa gggagtggtt ggtttgtgat tacacaacgt
    29641 caaaatgata ttttgaattt aatcgttgag ctatttacac aaactcatga accagtaggg
    29701 tctaaagctt tacagagaac gattgattct agtagcgcca ctattcgtaa tgatatggca
    29761 aaactagaga agttgggatt gttggaaaaa gcgcacactt ctagtggacg gatgccaagt
    29821 ccagctggct ttaagtattt tgtagagcac tccctcaggc tagatagtat tgatgagcaa
    29881 gatatttatc atgtcataaa aatttttgat tttgaagcat tcaaattaga agacatgctt
    29941 caaaaagcta gtcacatttt agcggagatg acgggctata catccgttat tttagatgtg
    30001 gagccagctc gtcaacgttt aacagggttt gatgttgttc agcttagcaa ccatgatgct
    30061 ctagcagtca tgactttaga tgaatctaag ccagtgacag tgcagtttgc tattccaaga
    30121 aactttttga cacgtgactt gatagcattt aaagctatcg tagaggagcg cttgctggat
    30181 agtagtgtca tagatattca ttataagttg aggacagaaa ttcctcagat cgttcaaaag
    30241 tattttgtga ctacggataa tgttttacaa ctatttgatt atgttttttc agagctgttt
    30301 ttggaaacgg tatttgttgc tggtaaggta aattctttaa cgtattcaga tctatcaacg
    30361 tatcagtttt tggataatga acagcaagta gcaatttctt tgaggcaaag tctgaaagaa
    30421 ggtgagatgg cttctgtcca agtggcagat agtcaagaag ccgcacttgc agatgtgtca
    30481 gttttgactc acaaattttt gattccttac cgtgggtttg gactcctgag tttgattggt
    30541 cctattgata tggattatcg taggtctgtt agtttggtaa acattattgg aaaggtattg
    30601 gctgctaaat taggtgatta ttatcgatac ctaaattcta accattatga agtccactga
    30661 taatggcagt attcaataag ttatttaaaa ggaggcactc agtgtcagaa gaaattaaaa
    30721 aagacgattt acaagaagaa gtggaagcca ctgaaactga agagacagtt gaggaagtta
    30781 ttgaagaaac tcctgaaaaa tcagagttag aacttgcaaa cgaacgtgct gatgagtttg
    30841 aaaataaata cttgcgagct catgcagaaa tgcagaatat acagcgtcgc tctagtgaag
    30901 aacgtcaaca acttcaacgc tatcgttctc aggatctggc aaaagcaatc ttaccatcgc
    30961 ttgacaatct agaacgtgcc ttggctgttg aagggttgac agatgatgtt aaaaaaggtt
    31021 tagaaatgac tcgtgatagc cttattcaag cactaaagga agaaggcgta gaagaagtag
    31081 aagttgactc atttgaccac aactttcata tggcagttca gaccctccca gccgatgatg
    31141 aacatccagc cgatagcatt gcagaagtct ttcaaaaggg atataaattg cacgagcgtc
    31201 tgctaagacc agcgatggta gttgtctata actaaggtaa atgagttttc gtttttgtcc
    31261 gtaatgacag taaactagat agcaagttag aagttatttc gcttgctgat taaactatag
    31321 tgattgctta gaattggaag taaaataatt cgagtgctta ctaagataaa ttgaaataaa
    31381 aagtaataaa gtattataaa ataagaggta ttaacatgtc taaaattatt ggtattgact
    31441 taggtacaac aaactcagca gtagcagttc ttgaagggac tgaatcaaaa atcattgcta
    31501 acccagaagg caatcgtaca actccttcag tagtatcatt caaaaatggt gaaattatcg
    31561 tgggtgatgc tgcaaaacgt caagcagtga caaacccaga aacagtaatc tctattaaat
    31621 ctaaaatggg aacttctgaa aaagtttctg caaatggtaa agaatatact cctcaagaaa
    31681 tttcagcaat gattcttcaa taccttaaag gttatgctga agactatctt ggagaaaaag
    31741 tagaaaaagc agttattact gttccagctt acttcaacga tgcacaacgt caggcaacta
    31801 aagacgctgg taaaattgca ggtcttgaag tagaacgtat cgttaacgaa ccaacagcag
    31861 ccgcacttgc ttatggtatg gacaagactg acaaggatga aaaaatctta gtttttgacc
    31921 ttggtggtgg tacatttgac gtatcaatcc ttgaattagg tgatggtgtc ttcgacgttc
    31981 ttgcaacagc aggtgataac aaacttggtg gtgacgactt tgaccaaaaa attattgatt
    32041 tcttagtggc tgaatttaag aaagaaaatg gcattgactt atcacaagat aagatggcac
    32101 ttcaacgctt gaaagatgct gctgaaaaag ctaaaaaaga tctttcaggt gtgacacaaa
    32161 cacaaatttc attaccgttc atcactgctg gttctgccgg tcctcttcac ttggagatga
    32221 gcttatctcg tgctaaattt gacgatctca ctcgcgacct tgtagaacgt acgaaaactc
    32281 cagttcgtca agccctttca gatgcaggat tgtcattgtc agaaattgat gaagttatcc
    32341 ttgttggtgg atcaactcgt atcccagcag ttgtcgaagc tgtaaaagct gaaactggta
    32401 aagaaccaaa taaatctgta aaccctgatg aagtggttgc tatgggtgct gctatccaag
    32461 gtggtgttat cactggggat gtgaaagacg ttgtccttct tgacgtaaca ccattgtcac
    32521 ttggtattga aacaatgggt ggtgtcttca ccaaattgat cgaccgcaac accactatcc
    32581 caacatctaa atcacaagtc ttctcaacag cagcagacaa ccaaccagct gttgatatcc
    32641 atgttcttca aggtgaacgc ccaatggcag cagataacaa gactcttggt cgcttccaat
    32701 tgactgatat cccagctgca cctcgtggaa tcccacaaat tgaagtaaca tttgatatcg
    32761 ataaaaacgg tattgtttct gtaaaagcta aagaccttgg tacgcaaaag gaacaacaca
    32821 tcgttatcaa atcaaacgac ggactttctg aagaagaaat tgatcgcatg atgaaagacg
    32881 ctgaagctaa tgccgaagcc gatgcgaaac gtaaagaaga agttgacctt aaaaacgaag
    32941 ttgaccaagc tatctttgct actgaaaaaa caatcaaaga aactgaaggt aaaggctttg
    33001 atacagaacg cgatgcagcg caatcagctc ttgacgagtt aaaagctgcg caagaatctg
    33061 gcaaccttga cgacatgaaa gctaaacttg aagcattaaa tgaaaaagcg caagctttgg
    33121 ctgttaaaat gtacgagcaa gctgtagcag ctcaacaagc agcacaaggt gcagaaggtg
    33181 cacaagctaa tgattcagca aataatgatg atgttgtaga tggcgaattt acagaaaagt
    33241 aatgatttag ttatctagta acattaatat ccgaattcag aggttgtacc aaacctctgt
    33301 ttttggctaa ataaaatgta aaaatgctga cgtcaaaata ttttaagaaa ggaatacaag
    33361 ttcgattatt cgaacacagg ctaaagcgtg taaagactct tttgcggatt cgaacacgcc
    33421 tacgactctg cgaaaaaaga gaaatacctc ctagatacga gagtatcttt gtcggatttc
    33481 ctatttttca ttgagtcgtt ttacggcttg gtatcttaca ttatgaacaa tacagaatat
    33541 tacgatcgtc tgggggtttc taaggacgct tctcaggacg atatcaaaaa agcttatcga
    33601 aaaatgtcta agaaatatca tccagatatc aataaagaag ctggagcgga gcaaaagtat
    33661 aaggatgttc aagaagctta cgaaacctta agtgattcac aaaagcgtgc tgcttatgat
    33721 caatacggcg ctgcaggtgc tcaaggcggc tttggtggcg gagcaggcgg ctttggtggt
    33781 tttgacggag gtggctttgg tggttttgaa gatatcttct caagcttctt tggtggcggt
    33841 ggaagccgaa acccaaatgc ccctcgtcaa ggtgacgatt tgcaatatcg tgttaattta
    33901 tcttttgaag aggctgtatt tggtgttgaa aaagaagtta gctataatcg cgaagcaacc
    33961 tgcggcactt gtttaggctc aggtgcaaaa cctggtaccg ctccagtaac ttgtcgtaag
    34021 tgccatggtt caggtgtaat gacaattgac acccaaacac cacttggtat gatgcgtcga
    34081 caagtcacct gcgatatttg tcatggtagt ggtaaggaaa ttaaagaacc ctgtcaaacc
    34141 tgtcacggta ctggtcatga aaagcaagct cataaagtgt ctgttaagat tccagctggt
    34201 gtagagactg gtcagcaaat ccgtttacaa ggtcaaggag aagctggctt taatggcgga
    34261 ccttatggtg atttatttgt tattttgaat gtcttaccaa gtaagcaatt tgaacgcaat
    34321 ggttccacta tttactacaa cttagatatt tcttttactc aagcagcctt aggagatact
    34381 gttgaaattc caacggttca tggcgatgtt gagatggcta tccctgctgg gacacaaaca
    34441 ggtaaaacct tccgcctcaa aggcaaagga gcaccaaaac tccgtggtgg aggtcaaggg
    34501 gatcaacatg ttaccgttaa tattgtgacc ccaaccaaac tcaatgacgc tcaaagggaa
    34561 gccttgcaag catttgcaga agcgagtggt gaaaaaatgt tacatccaaa gaaaaaaggt
    34621 ttttttgata aagtgaaaga tgccttagag gatatttaaa gatgtcaaac gtataatctc
    34681 aggataattt tcccgaagtt atacgttttt ttacgtcaaa acagttagag aaaacgtttt
    34741 cttttataga tatcgttttt ttctcttgtc aaaatggttt atttagtata tcataattag
    34801 gtaattgatt tgattatcaa atgattttaa agagaaataa tttcttagtc aaataaatta
    34861 tcagaaaaga ggattgttat gcagtttaaa cacatcatct ttgatgttgt agatgatctt
    34921 gcgacattga ctttaaaccg tccagaggtt tcaaacggtt ttaatattcc aatttgtcaa
    34981 gagattctgg tagcattagc agaagttaaa cgcgatacat ctgtgcgttt tttattaatt
    35041 aaagccgttg gaaaagtgtt ttcagttggt ggtgacctag ttgaaatgca agaagctgtt
    35101 gcaaaagata atgtgcagtc gctagtcaag attgctgaac tggtacaaga aatttctttt
    35161 gcaatcaagc atttgccaaa accagtcatc ctttgcgccg atggagctgt agctggagcg
    35221 gcttttaata tagctttagc tgttgacttt tgtatcgcta gtactcaaac aaaatttatc
    35281 caagcttttg taaatgttgg actagcaccg gatgccggtg gtttatttct attgactcgt
    35341 gcagtaggac ttaatcgtgc aactcacctt gtgatgacag gtgaaggaat tactgctgat
    35401 aaaggtctcg attatgggtt tgtctaccgt acggcagaat ctgataaact agacaaagtt
    35461 tgtcttcaac ttttaaagcg attgagacgt ggctcttcaa attcttatgc tggaatgaaa
    35521 tcattggtct ggcaaagctt tttcacagga tgggaagatt atgctaaggc agagcttgct
    35581 attcaagagg agctagcttt caaggaagat tttaaagaag gtgtcatagc ttttggagaa
    35641 aggcgccgac caaattttca aggtaagtaa gacagctgtt gcttgcaaaa tactttgacc
    35701 tttgatataa tttgattgtc aaagtttatt gctaggaggt gtttacttgg aatatgacaa
    35761 aattaaccaa tacttagttg atattttcaa ccggattttg gttattgaag aaatgagctt
    35821 aaagacgagt cagtttaatg atgtctcttt aaaagagatg cacactattg aaattatcgg
    35881 caaatatgat caggtgactc caagtgatat cgccagagaa ttaatggtaa cgttagggac
    35941 agtaacgact agcttaaata aattagaagc aaaaggttat atcgcaagaa cgcgatcacg
    36001 ctcagatagg cgagttgttt acctgtcatt aacaaaaagg ggtcgacttt tggaccgcct
    36061 ccatgcaaag ttccataaaa atatggttgg acatgtcatt gctgatatga gtgatgaaga
    36121 gatgcaggca ttggtgcgtg gactagggaa tctacatcaa tttttggagg atttggttta
    36181 aatgattttt tctaaaatca gtcaggtagc ccactatgtt ccacagcagt tagtgactaa
    36241 taatgattta gctagcataa tggacactag tcatgagtgg atcttttctc gaacaggaat
    36301 agcagaacgc catatttcta gagatgaaat gactagtgac ctagctattc aggttgctga
    36361 tcagctttta actcagtctg ggttaaaagc tgatgctatt gattttatta ttgttgcaac
    36421 tatcagccct gatgccacta tgccatcaac agctgcgaaa gttcaagcag ctattgcggc
    36481 tacaagtgct tttgcttttg atatgactgc tgcttgtagt ggctttgtct ttgccttggc
    36541 tatggcagat aagcttattg catcaggtgc ctaccaaaac ggaatggtaa taggtgcaga
    36601 gactttatct aagttagtaa actggcagga tcgagcgaca gccgttcttt ttggagacgg
    36661 agctggtggc gtgctactgg aagccagtaa agataaacat gttttagcgg aaacattaca
    36721 cacagatggt gcgagatgtc agagtttaat ttcaggagaa actagtcttt catctccgta
    36781 ctcaattggc aaaaaagcca tagcgactat tcagatggat ggacgtgcca tatttgattt
    36841 tgcgattagg gatgtgtcaa aaagcatcct cacactaatg gcgcaatcag atattacaaa
    36901 agatgacata gactattgtt tattgcatca agctaatcgt cgtatacttg ataaaatagc
    36961 acgcaagatt gatgttcctc gggaaaaatt ccttgaaaac atgatgcgtt atggtaatac
    37021 tagtgctgct agtatcccaa ttctcttgtc agaggctgtt caaaagggac agattaggtt
    37081 agatggtacc caaaaaatct tactttccgg ttttggtgga ggtttgacat ggggaagtct
    37141 aattgttaaa atctagttat tcatgtgcct agcacattta taaaaacaaa tttatatttt
    37201 ttaaggagaa ataaacatgg cagtatttga aaaagtacaa gaaattattg ttgaagaact
    37261 tggtaaggag acagaagaag taacgctaga aactacattt gatgatttag atgcagattc
    37321 acttgatgtt ttccaagtta tttcagaaat tgaagatgct tttgatattc aaattgaaac
    37381 agaagaagga ttaaatactg ttggtgacct tgttgcttac gttgaggaaa agtcaaaata
    37441 aggataaaag tgacaagtaa catcagaagt ctatgaaaag cttactgctg ttatgttcat
    37501 gttcatcact aaaaaaaaca ataagtatta atcaaaccat tagggtggga ttagactctt
    37561 tttaggatct agctcgctct ttcttcttac tataatagtt tgattatcaa attattctcc
    37621 ttatttttta tagaaagtta ttatgaaaac acgtattaca gaattactta atattgatta
    37681 ccccattttt caaggaggaa tggcttgggt tgctgatggt gatttagcag gtgcagtttc
    37741 taatgctggt ggtttaggca ttataggtgg tggcaatgct cccaaagaag tcgttaaagc
    37801 taatattgat cgtgtcaaag ctattactga tagacctttt ggggttaata tcatgctttt
    37861 atctcctttt gctgatgata tcgttgatct ggtcattgaa gaaggtgtta aagtagtaac
    37921 aacaggcgca ggaaatccag gaaagtatat ggaaagactg caccaggcgg gtataatcgt
    37981 tgttcctgtt gtcccaagcg ttgcgctagc caaacgtatg gaaaagcttg gggtagatgc
    38041 agtgattgct gagggtatgg aagctggagg acatattggc aagttaacga ctatgtcttt
    38101 agtaagacaa gttgttgaag cggtttcgat tcctgtcatt gcggcaggtg gtatagctga
    38161 tggtcatggt gcagcagcag catttatgtt aggagcagag gctgttcaaa ttggaactcg
    38221 ctttgttgtt gctaaagaat ccaatgccca ccaaaatttt aaagataaaa tcttagcagc
    38281 aaaagatatt gatacggtga tttctgcgca ggttgtgggc caccctgtcc gctctattaa
    38341 aaataaattg acctcagctt acgctaaagc agaaaaagca tttttaattg gtcaaaaaac
    38401 agctactgat attgaagaaa tgggagcagg atcgcttcga catgctgtta ttgaaggcga
    38461 tgtagtcaat ggatctgtta tggctggcca aattgcaggg cttgtgagaa aagaagaaag
    38521 ctgtgaaacg attttaaaag atatttatta tggtgcagct cgtgttattc aaaatgaagc
    38581 taagcgctgg caatctgttt caatagaaaa gtagataaac acatgaagga taatgataat
    38641 gacaaagaca gcctttttat ttgccggtca aggtgcccaa aaattaggga tggcaaggga
    38701 tttttatgat aactttgcta ttgtaagaaa aacctttgat caagctagtc aagtattggg
    38761 atacgatttg cgccgtttga ttgatagtga cgagttaaaa cttaatcaaa ctagctatac
    38821 gcaaccagct attttgacat catcaattgc tatttaccgt gttctgggct tacatcacgt
    38881 taaaccggat atggtagctg gtctttcctt aggagagtac tcagctttgg tagcatcagg
    38941 ggcactctct tttgaagata ccctatcctt agtagctaag agaggccgct tgatggagga
    39001 agcagcacca caaggatctg gaaagatggt tgccgttatg aatacagacg tgcaagtcat
    39061 cgaagaagtc tgtcaaatag ctgctaaaca tggagtggtt gctccagcaa actataatac
    39121 tcctagtcaa attgtaattg gtggtcagac agatgctgtg aacgtggcag ttgaactttt
    39181 aaaggaaagg ggagttaagc gtttaatccc tttaaacgtg tcaggtcctt tccacactgc
    39241 tttgttagaa ccagctagcc gcttgttggc taaagagttg gaaagataca acttcagtga
    39301 cttcaagatt cctttggttg gtaataccga agctaatatt atggaaaaag atcgtatccc
    39361 agaactatta gcccgtcaag tcatggagcc tgttcgtttt tatgacagtg ttgcgacttt
    39421 agtagaaagt ggcataacac aattcattga ggtaggacca ggtaaggttt tgacaggttt
    39481 tgtgaagaaa attgataaaa atttactatg tactagtgtt gaaaacatgg taagcttacg
    39541 tttattttta gatagagagg atattcatgg aaattaaagg aaaaaatata tttatcacag
    39601 gctcaacgag aggtatcggt ttagcaatgg ctcatcaatt cgccagttta ggagctaata
    39661 ttgtcttaaa tggtcgctct gctatctctg aggagcttgt tgctagtttt actgactatg
    39721 gtgttacagt tgttaccatc tctggggatg tttcagaagc atcagaagct aaacgtatgg
    39781 tcaatgaagc tatcgaaagt ttaggttcga ttgatgtttt agttaacaat gctggcatta
    39841 ctaatgataa attgatgctt aaaatgacgg aagaggattt tgagcgcgtc ttgaaaatca
    39901 atttgaccgg tgcttttaac atgactcaat cagtgttaaa accaatgatc aaggcccgcc
    39961 aaggagcaat tattaatgtc tctagtgtgg ttggtttaac aggcaatatt gggcaagcaa
    40021 actatgctgc ttctaaggca ggaatgattg gttttaccaa gtctgttgcc cgtgaagttg
    40081 ctgctcgtaa catttgcgtg aatgctattg ctccaggatt tatcgagtcc gacatgacag
    40141 gtgtccttcc tgaaaaaatg caagagcaaa tcttaagtca aatcccaatg aaacgtattg
    40201 gaaaagctca agaagttgct cacttagcaa gcttcttggt ggaacaagat tatattacgg
    40261 gacaagttat cgctattgat ggcggtatga caatgcaata aggagacgta gtgaaatgac
    40321 atttaaaaga gtagtggtaa caggttatgg tctaacatct ccaatcgggc atgatcctga
    40381 aacgttttgg aacaatctga aagctggcca aattggtatt ggtcctatta ctaaatttga
    40441 cacgactgat tatgcagtca agaatgctgc tgaaattcag gattttccat ttgacaaata
    40501 ctttgtgaaa aaagatttga accgtttcga tagatattct ctttatgctc tttacgcagc
    40561 gaaagaggct attaatcatg ctgatttaaa tattgagatg gttgattccg atcgatttgg
    40621 cgttattgtt gcttcaggta ttggtggaat tgcggagatt gaagagcagg tgattcgctt
    40681 acatgaaaaa gggccaaaac gtgtgaagcc aatgacgttg ccaaaagcgc taccaaatat
    40741 ggctgcagga aatgtcgcca tgtctttaaa agctcaagga gtatgtaaat caattaatac
    40801 tgcctgcgct tcttcaaatg atgcgattgg ggatgccttt cgtgccatta aatttgggac
    40861 tcaagatgtg atgatagttg gtgggtcaga ggcggccata accaagtttg ccattgctgg
    40921 cttccaaagt ttaacagctc tctcaacaac tgaagatcca agcagaagct ctattccatt
    40981 tgataaagac cgaaatggtt ttatcatggg agaaggatca ggaatgcttg ttttagaaag
    41041 cctagaacat gcgcaagaac gcggggcgac aatcttagca gaaattgtgg gttatggaaa
    41101 tacctgcgat gcctaccata tgacatctcc aaatcctgag ggattaggag ctcgtaaagc
    41161 catccactta gccttacaag aagctggtat tgaagccagt gctattaatt atgtcaatgc
    41221 gcacggaact tcaactccag ctaatgaaaa aggagagagt caagcaattg tagccgttct
    41281 tggtaaggat gttccggtgt cttcaacaaa atcgtttacg ggtcaccttc ttggagcagc
    41341 aggcgctatt gaagctattg ccactatcga ggctatgcgt cataactatg ttccgatgac
    41401 ggctgggaca caagctttat cagaggatat cgaagcaaat gtcatctttg gagaaggaaa
    41461 agaaacagct atcaactatg ccatttcaaa tacttttgga tttggtggtc ataatgctgt
    41521 attagctttc aaatgttggg aggagtaggt tgaatattca agaaatcaaa gatttgatgg
    41581 ctcagtttga tacctcaagc ctacgtgaat ttttattcaa aacaaatgaa ggtgagctaa
    41641 tctttagtaa aaacgaacaa cacctcaatg catcgacatc aaatcaagaa catgctgtgc
    41701 ctgtaccaca ggtacaactt gttccgaatt caactgcttc ggaagcgtcc agtccagcat
    41761 cagttaagga tgttcctgta gaggagcaac cacaagcaga atcttttgta gcagaaggtg
    41821 atattgttga aagtccttta gtcggtgtag cctatcttgc agctagccct gacaagccac
    41881 catttgtggc agtcggagat actgtaaaaa aaggacaaac ccttgtgatt atcgaagcca
    41941 tgaaggtgat gaacgaagtg ccagcgccat gtgatggtgt gattacagaa attttggtat
    42001 caaatgaaga tgtcattgaa tttggacaag gattggtgcg gatcaaatga tggatattag
    42061 agaaattcaa gcggctttgc ctcatcgcta cccaatgtta ttggttgatc gtgtgttaga
    42121 agtgtcggac gaccatatcg ttgccattaa aaatgtgact attaatgaac ctttctttaa
    42181 cgggcatttt cctcactatc ctgtgatgcc aggtgttctg attatggaag ctttggctca
    42241 aacagctggt gtcttagaat tgtcaaaaga agaaaataaa ggcaagttgg tcttttacgc
    42301 tggaatggat aaagtgaagt tcaaaaaaca agtcgtccct ggtgatcaac ttgtgatgac
    42361 agcaaccttt atcaagcgtc gcggtaccat tgctgtcgtt gaagccaggg cagaagttga
    42421 tggcaagcta gcagcaagtg gaacattaac ctttgcctgt gggcaatgag aatgattgtt
    42481 ctgcggagga agaaaccctt atgtttaaaa aaatcttaat tgctaatcgt ggtgaaattg
    42541 cagtgcgtat cattcgtgct gcacgagaat tagggatttc cacagttgca gtttattcgg
    42601 aagcggataa ggaagctctg cacacgatat tggcggacga ggctatctgt attggtcctg
    42661 cccgttccaa ggaatcctat cttaatatga attccgtctt gtcagcagct attgtgacag
    42721 gagcccaagc gattcaccca gggtttggct ttttaagtga aaactcaaag tttgcgacca
    42781 tgtgtgaaga gatgaatatt aaatttatcg gtccatccgc atcggttatg gataaaatgg
    42841 gtgataaaat caatgctcgt tctgaaatga ttaaggcagg agtgcccgtt attccagggt
    42901 cagatggtga agtttataac gctcaagaag ccttagctat tgccaataag attggttatc
    42961 ctgtcatgct aaaagcatcg gcaggtggtg gaggtaaagg gattcgtaaa gtggagacag
    43021 aagcagattt ggaggcggct ttcaatgctg ccagtcaaga agctttggga gctttcggaa
    43081 atggcgccat gtatttggaa aaagtaattt acccagctag acatattgag gtgcaaattt
    43141 taggagacgc tcacggtaac atcattcact tgggagaacg ggattgctcc ttgcaacgga
    43201 ataatcaaaa agtcttggaa gaaagtccat ccattgctat tggcaatacc ctcagacacg
    43261 aaatggggca agcagcggtt agagcagctg aagctgtagc ctatgaaaat gcgggaacca
    43321 ttgagttttt gttggacgaa gacagcgaga aattctattt catggaaatg aatacccgta
    43381 tccaagttga gcatcctgtc accgaatttg tgacaggagt ggatattgtc aaagagcaaa
    43441 tcaaaattgc agcaggtcag cctttggcga ttagtcaaga agacatcact atcacgggac
    43501 atgctattga atgtcgtatc aatgctgaaa atactgcttt taactttgca ccgagtccag
    43561 gaaagattac agacttgtat atgccaagtg gtggtgtagg actacgagtt gattcagcag
    43621 tttacaatgg ctatgccatt cctccttatt atgacagcat gattgctaaa atcatcgttc
    43681 atggaagcaa tcgatttgac gctttgatga aaatgcaacg tgccttagtt gaattggaaa
    43741 ttgaaggcat tatcaccaat acagacttcc agctcgattt gatttcagat aagcgagtga
    43801 ttgcaggaga ttatgatacc tctttcttga tggaaacgtt cctgccacat tacaataaag
    43861 attaggagaa aatatggctt tatttcgtaa aaaagataaa tacatccgta ttactcccaa
    43921 taattccctc aaaggatctg tcagccataa tgtcccagaa gttccagatg aactctttgc
    43981 gaaatgccca gcttgtaagc acatgattta taagaaagat ttgggattgg caaagatttg
    44041 cccaacctgt tcttacaatt ttaggatttc tgcccaagag cggctgacct taacagttga
    44101 tgaaggctct ttccaggaat tatttacaag tattgaaacc aaggatccgt tacgctttcc
    44161 aggttatcag gaaaaattac aaaaagccaa ggaaacaact ggtctgcatg aagctgtttt
    44221 gacagggaaa gctatggtta aaggacaaca aattgctctt gctattatgg attctcactt
    44281 tattatggct agtatgggaa ctgttgttgg ggaaaaaatt acccgattgt ttgaattggc
    44341 tatcgaagaa aacctgccgg tggtgatttt tacggcttca ggaggcgccc gtatgcaaga
    44401 ggggatcatg agccttatgc aaatggccaa ggtctctgca gctgtgaaaa gacattctaa
    44461 tgcaggcctc ttttacctca ctattttgac agatccaact actggtgggg taacagctag
    44521 ttttgccatg gaaggggata ttattttggc agaacctcaa tccttggttg gctttgcagg
    44581 tcgtcgtgtc attgagacca cagttcgtga aaacttgcca gatgatttcc aaaaagcaga
    44641 atttttacaa gaccatggtt ttgtggatgc tattgtgaaa cgaacagagt tgcgagacaa
    44701 gattgcccac ttagtagcat ttcatggagg tggccaatga cagatgtatc aagaatttta
    44761 aaagaagcgc gtgatcaagg gcgtttaaca actttggatt acgccaacct tattttcgat
    44821 gactttatgg aactgcatgg cgatcgccat ttttcagatg atggtgctat tgtaggtggc
    44881 ttagcttatt tggcgggaca acctgttacg gtcattggta ttcaaaaagg taagaattta
    44941 caggataatt tggcaaggaa ttttggccag cccaatccag aaggttatcg taaagctttg
    45001 cgccttatga aacaggcaga aaaatttgga cgaccagttg ttacgtttat caatactgca
    45061 ggagcctatc caggtgtcgg tgcggaagaa cgaggacagg gtgaggccat tgctaaaaat
    45121 ttgatggaaa tgagtgatct caaggttccc attatcgcca tcattattgg tgaaggaggc
    45181 tctggtggtg cattagcctt agcggttgcc gatcaggtct ggatgctaga aaacaccatg
    45241 tatgcagtcc ttagtccaga aggctttgct tctattttat ggaaagatgg ttcaagagcg
    45301 acagaggctg ctgaattgat gaaaattacc gcaggcgaat tgtatcaaat gggcatagta
    45361 gaccgtatta ttccagaaca tggttatttt tcaagtgaaa tcgttgacat catcaaagct
    45421 aacctcatcg aacaaataac cagtttgcaa gctaagccat tagaccaatt attagatgag
    45481 cgttaccaac gctttcgtaa atattaacaa catccttttg gagtctccct acgtgtttat
    45541 caagctaagg gaggcctttc caaaagggtt tttaactagt cttgtaaata aggaaatggg
    45601 tatactaata agacttttga taaactagtg tgaaaaaaag tgttgttaaa ggggcaatgt
    45661 agtgagtttc aagtgatgtg gtggctcttt aaaatagtaa accccagaaa tcactttaat
    45721 gatctctggg gtgagtttat tatttaggtg aaatgacggt ttcgccaccc atgtaagagc
    45781 ggagtacttc agggatggta acagaaccat cttcattttg gtagttttct aaaatggctg
    45841 ctacggtacg accgacagca agtcctgacc cattaagggt gtgaagaagt ttgaccttac
    45901 cgtcagcttc atcacggtaa cggatttgag cacgtcgtgc ttggaaatct tcagtatttg
    45961 aacatgatga aatttcacgg taggtgtttt gtgcaggaat ccagacttca aggtcatagg
    46021 ttttagcagc tgagaagccc atgtctccag tacaaaggga aatgacgcga tatggcaaac
    46081 caagtttttg aagaatattt tcagcattag cagtcatttt ttctagttct tggtaagatt
    46141 cttctggttt agcaaattta accatttcaa ctttgtggaa ttggtgtagg cggataaggc
    46201 cacgtgtgtc acgacccgca gaacccgctt cagaacgaaa agatgggctc attgcagtaa
    46261 agtaaattgg caattctttg ccgtctagga tttccccacg gtaataattg gtcaaaggca
    46321 cttcagcagt tggaatcagg acaaagttgg tatctgctaa ttcaaaggtg tcctctttga
    46381 actttggata ttgtcctgta ccaaacatgg aatcgtggtt aaccatgtaa ggtgtgatga
    46441 tttcttgata accttctttg atatgttcat ccagcatgaa gttataaaga gcacgctcca
    46501 aacgagcacc aagattttta tagaataaga aacgagcacc agttaccttt gccccacgtt
    46561 cccagtctaa aatatcaaga tcttcgccta gatcccaatg agccttaact tcgaagtcaa
    46621 agtcacgagg cgttccccaa cggcgaattt cgacattgtc ttcttcgtca gctccaacag
    46681 ggacagaatc gtgaggagta ttaggtaaaa cagtgatgat gtcagtaact tgctggtcga
    46741 tagctactag ttgattgtca atagttttga tatctgcaga gactttttgc atatcagcaa
    46801 tttgttgagt ggcatcttct ttttgacgtt ttgcttgggc gatggctgca gaagcaatat
    46861 tacgctctgc ttttaattct tctgattgca ctaatagagc acgacgtttt tcatcaagtt
    46921 ctttaagatg ggtcagggtg tcttcagaaa caccgcggtt ttttaatttg gcagcgacag
    46981 tgtcaaaatc ggtacggata cgtttaagat ctagcataca gaagctcctt taagatagtt
    47041 attagtagct gtcagtgaaa caaggctaag taagacagtt ggatcttgtg attctctgac
    47101 gatagaaaaa gcacagctta tgaagatgct ggagcgcttt ggcgcggttc catccagctt
    47161 cacaatctgt gcacttaatc tctattttat tgatgacacg gtagaatttc atatcatttt
    47221 ccctatctgt tcacagcatc cacagactct ctgaatgaaa aaaaggtatt acttatccgt
    47281 taggactatt ataacaaaaa acaagaaaaa gtaaactcaa atgagggaat catttccact
    47341 aaatagcaat ttagagctac tattctttgc tttcttgaaa tgataaatca gcactgctca
    47401 ttattttttt aatgtcgaaa aaatttgtta ataaaggttt gcatcaaggt tggtaatgta
    47461 atcactttct cattaccaat atgttgacga gtgattttta agatttttcc agagtctcca
    47521 gaagcaaata aaaacttgcc ttggtccgta aaaatttcaa agtggcggct aacttttttt
    47581 cttgaaacat tagccccgat gtgattgata gctgtccaag gaatttgaat ataatcattc
    47641 acatttttat cgttgtaaaa ttcaagggcc ttgtcaccga gtaaaatatg accaactttg
    47701 cctcctattc caaggtagga gacagctttt gtcttgtatt cgacactagt gtttaaagat
    47761 tgagccattt aagttccttt cattgcgtag ttgtagttat tataccatat tttaagtggg
    47821 attttcctga cataaagcat aaaaaagaag cctgagttca tctcagactt ccattggttg
    47881 attacataat acctgcaagg tgagcaagga taccaaatgc aaacaagccg atgatgattg
    47941 cgattggaga aacttttttc ttcaataacc acatgcaaag gaatgtaagg agtaatccag
    48001 ctaaacctgg gataagagag tccaattgtc cttgaagggt ttgagcttgg actttatcaa
    48061 ggctcatacc gccaatagcg ttggcaagga tagttttcaa ctcagcacct ttaacagcgc
    48121 catctgggaa gaccacatag gcaccatctg acaattgttt tccaggaagg tcgatagtaa
    48181 agttgataga tacccaacgt tgaacaagca ctgcaaggat aaacattcca aggattgaag
    48241 cacctttagt aatgtcttgg aggataccgc ctgacatgtc tttagtaatt tcagaaccag
    48301 ctttgtaacc aaattcttga gtataccata agaaggccat acggataagg ttccatccaa
    48361 agaagaacag aagtggacca acgatgttac ctgttgaagc caaagaagca ccaagtgccc
    48421 caaggatagg acgaaccgta aaccagaata ctgggtcacc gataccagca agaggtccca
    48481 tcataccgat tttaacccct tggatagcct tgtcatcaat tggcgtacca tttgcgcgtt
    48541 cttcttcaag ggcaagtgtt acaccaatga ttggagctgc aacgtatggg tgagtgttaa
    48601 agaattccat gtggcgctca agagcagctg cacggtcttc aggagacgta tacaattttt
    48661 taagagcagg gataagtgca taagcccagc ccatgttttg catacgttcg tagttccaag
    48721 aaccttgtaa gaattgtgaa cgccaccaaa cacgttgacg gtctgattta gttaatttaa
    48781 tttgttcagt catttgttct tacccctttc tagtagtctt ctaagatgtc gccgattgga
    48841 tcagctgaac ctgctgcatt accaccgtta ccaccttttt tagaaagatt aaggtagatg
    48901 aaggcaattg ctacaccgat aacacccata gcgataaggg ttaattggct gatagctgca
    48961 agagcaaaac ctagggcgaa gaatggccat acttcacgag tagccatcat gttgataacc
    49021 atagcataac ctacagcaac aaccatcgca ccaccgattt gcataccttc gttcaaccag
    49081 tttggcatag cattcaaaac agattgtacg gctgaagtag gaacagcaac aagaagagct
    49141 gctgggacag cgatacgcaa tccttgaaga agaagggcaa taaagtggaa gcgttcaata
    49201 cctgcgaaat taccttcagt ggcagctttg tcaccagcgt gtacaagggc agttgacaaa
    49261 gtacgaacga tcattgtaag gaaaagacca gcaacagcaa gtgggatagc tgttgagtaa
    49321 gcaaaggtaa ttcctttttg agtaaagtcg ccacttttga ccatgatgat agcagctgca
    49381 acagaggcaa gggcagcatc aggggcaacg gcagcaccga tgtttgccca accaagagca
    49441 agcatttgaa gtgtaccacc aaggatgaca ccagcttcaa ggtgtccagt tacaagtcca
    49501 atcaaagtac aggcaacaag tggttggtgc atttgaaatt ggtcgaggat accttcaaga
    49561 ccagcgaaga aggcaatgat aacgactaaa attgcagaaa taatcgaaat atctgacatg
    49621 ttgtaatcct ttcaaagtat agtaaacgag gactttttgc taataagtta gctagtcatc
    49681 gttttaaaac cttatagcgt ttgaaactta tttaatgtta gctttttgga taagctcaaa
    49741 taagtttttc ttagaatcgt taggaacttt acggacgtcg aatgtaacac ctaggtcgcg
    49801 taatttttca aaagtagcaa cgtcttcttt gtccattgat aaaacgttgt taaccattgt
    49861 tttaccagtt gagtgagcca ttgaaccaac gttcaattcg ttgatttcaa caccaccttc
    49921 aaccgcgcga agggcatctt gaggggtttg gaaaagaata agtgcgtgtg tgttaccaaa
    49981 acgaggatct ttagaagctt caatcaattt gctaattgga acaacgttag ctttgacacc
    50041 accaggtgca gcctgtttga tcaattgttt gcgaaggtca tcttgagcaa cctcatcaga
    50101 agcaacaatg atacggtcag ccttagaagc tggagtccat gctgttgcaa cttgaccgtg
    50161 taaaagacga gtgtcaacac gagcaagatt gatctttaat ttaccatctc cgatgacagt
    50221 tccagcagga atagctcctt gaagtgcgtt aacaactttt tcagttgcag cagtttcttc
    50281 aactgggtta aggtcttcag gaagggcttt gataccgtcc ttagattcct taatgatgtt
    50341 tgcagcaact tgttcaacac ctgcaccagc atccataaga cgttcagtgt aagcttgaat
    50401 aagcataggc aagttgagac cagtgatgat cgccattttg cggtcaggat tttcacctgc
    50461 cacacgacta gcttggttaa atggagaacc actccaaagg tcagctagca caaggatttc
    50521 atcatcagca tcaaattgtt ggatagcatt gttgaagtgt ccatacaaat catctggtcc
    50581 ttcgtttggc atgaaagtaa caacttgaac tttctcttgt tcaccaaaaa tcatagaacc
    50641 agattgatga ataccttcag caaatttacc atggctggca ataataatac cgatacccat
    50701 tcttgttctt cctcctttaa aatatttttc gttgaaatga agaagaataa tctccttcca
    50761 aaactaaatt ttggcaattc tttttcactt aaacgaaatt caagaacgtt tctattttaa
    50821 aacgttttca ctaaaaaatc aagccctttc ggaaaaatag tacaaaacat gaaaaaattt
    50881 tcagaattta ggattaaata ggcagattat tgatattaaa ggtgttttca agctgtttaa
    50941 aacaaaaata tgctgaaaaa tgttgaaatt attaaaatga ttttcatttt ttgatagctg
    51001 ttgacagaag aaaaaccacc attttctgaa aaatagtggt ttcgtttgct actacaagaa
    51061 aagttcttcc aattttttgg caacaccgtc ttcttcgttg gaaaaattaa gctgctgatc
    51121 agcataaggc agtagcacag ggctagcatt tttcatagca taaccagttc cagcaaaagc
    51181 tagcatctca gtgtcattgt gttcatcacc aaaggcgatt aaatcctttt tatccatatt
    51241 gtaaattcca aggagatagt tcagagcgta agctttattg acattcttag acgaaatttc
    51301 aagaatgttg agagggcctc cccaagaatc aatttcaatt tcatctttga agaaagcacg
    51361 catgttttta gctaaggcat acttgtcttc gtgatgcgtt tgcatcaata aggcgtttgg
    51421 atttcgagtg attttggtaa tctctaatgc catgtcctga gtaatttcat caacaccaaa
    51481 gagttgaggg tcgatacttt ctggattggt catggtgatg tagacatttt ttcgatactc
    51541 actggcaata aaatccatct ggaaatcatc ctgatatttt agtaacctta gtaggtagcc
    51601 tttgtccaaa gtgacattat gttcataggc ccatttttgt tcaggcatgt gggtcaaggc
    51661 cccattaaaa gtaatgattg gggtctttaa attgagctgc agatagtagt ccagtgccat
    51721 gcggtaagga cggcctgttg aaataatgac gtggtggccc ttgtcttgga cagctttgat
    51781 gattttttga gtataggtag agatggtgtt gtcatgatgt aataaggttc catccaaatc
    51841 aatggcaatc aattttttag tcataattaa attatagcaa attttgctcc aagataaaga
    51901 gaaaagataa gttaagcgta ttcattagta aaaaagatag gataactgtt tttttaatga
    51961 ataaatgaag ttacagacgt tgaaaagtga acaaaaaaat ggtaacatag tgttattgtt
    52021 cattttaata cgaacaataa caataataat gaggtgtcaa aaagacttat ctagtgataa
    52081 gcgctttttg attttaccta ctatttaatt aaggagactc catttcaatg gaaaagtttt
    52141 ttaagttaag cgaaaatggg acaactgtct caactgagat tatggctggt ttaacgacct
    52201 tttttgccat gtcctatatt ttgtttgtta acccaagtat tttaggtgca gcggggatgc
    52261 cctctaatgc tgtctttttg gctacgatta tcgcagcagc catatcaacc ttaattatgg
    52321 gactatttgc caatgtgcct tatgcgttgg caccaggaat gggacttaac gcttttttca
    52381 cttatacagt tgtttttgct ttagggtttt catggcaaga agccttggca atggttttca
    52441 tttgtggatt attcaatatt tttattaccg taaccaagtt tcgtaaaagt atcatcaagg
    52501 cgattccagt tagtttacag catgctattg gtgggggaat tggtgtcttt gtagcttatt
    52561 taggatttaa aaacgcaaat atcattactt tttctatctc tgctgaaaat atagtaatgg
    52621 taaatggtgt tgaaccggct aaagcatcgg ctaaaacatt tgcagatggt ctattatttg
    52681 tagacgccaa tggtggagtt gtacctacga tttctagttt tacggattct agtgtattac
    52741 ttgctatttt tggtttactt ttaacgacag ctcttgtgat tcgaaatttt agaggtgcta
    52801 ttttaattgg tattgtcgca acaactcttg taggtattcc tttaggaata gtggatgtgt
    52861 ccaacctcaa ttttgggatc agccatattg gtgaagcttg gactgaatta ggtacaactt
    52921 tccttgcagc tttcgatggt ttgagttctc tttttagcga ttcaagtcgt ttaccgctag
    52981 ttttcatgac tatttttgct tttagtctat cagatacttt tgacacaatt ggtaccttta
    53041 tcggaactgg tcgtcgaaca ggtattttct ctcaagacga tgagaatgct ttggaaaata
    53101 gtataggctt tagttcaaaa atggatcgtg cgctttttgc agatgctatc ggtacttcta
    53161 ttggggcttt ggttggaact tcaaatacga ctacctatgt tgaatcagca gcaggaattg
    53221 ctgaaggtgg acgtactgga ctaacagcag tctccaccgc agtatgcttc ttattatcaa
    53281 tattgctatt accgcttgta ggtattgtcc cagctgctgc tacggctcca gctttaattg
    53341 ttgtgggtgt catgatggtg tcttcttttc ttgatgttaa ttggagtaaa tttgcagatg
    53401 ctcttccagc tttttttgca gctttcttta tggcgctgtg ttactctatt tcctatggta
    53461 ttgccgctgc ctttattttc tattgtctag taaaagttgt tgagggaaaa acaaaagata
    53521 ttcaccctat tatttgggga gcaaccttct tgttcattgt aaatttcatc atattaacta
    53581 tcttataacc aaataattta cttattttgc tactaactgc cgcttcttta attgatttta
    53641 acctttaata taacgaagcg tttttacttt acgaatacct tattggagtc atcttctagg
    53701 tagtgcttgg aagtatgttt tttttctgat ataatgttgc tatgttttat agtgaaaatg
    53761 aatatactct taaagcttac ggagaaaccc taggtactta tttgtctatt ggagatgtga
    53821 ttgtattgtc tggcgactta ggtgccggta aaacaacatt ggcaaaaggt attgctaaag
    53881 ggatggggat tagtcaaatg atcaaaagcc caacttatac tattgttaga gaatatgaag
    53941 gacgtctacc tctttatcac ttagatattt accgtgttgg tgatgatcca gactctattg
    54001 atttagatga ttttcttttt ggtaatggtg ttaccgttat tgagtgggga gaattattag
    54061 gagagggatt attacaagac tatttgcaaa taaccattac taaacgagat aaaggtcgtc
    54121 aactagactt gttagcgcat ggtgagcgct ctcgtcagct tctggagata atgtcacatg
    54181 attcagcaaa gtctcgttat tgaagaaggc gtacctcaag atgcaaaagc tgtactttcc
    54241 ttagtggcgg cagctgccaa agaaacagat tttataacag gcgtagagac tatattagag
    54301 gctactcctc atgaattatc agacttttta tcaagaagtc aaacctcttt tattgatttt
    54361 tgcctgctag ctagattaga tgaaaaagta gtagggcttt taaatctttc tggggaagta
    54421 ttatcccaag gccaagttga agcagatgta tttatgttag ttgctaagac ttaccgtggt
    54481 tatggtatcg gtcaattatt gttagagatt gctcttgatt gggctgaaga aaacccttat
    54541 atcgaaagcc ttaagttgga cgttcaagta agaaatacta aggctattta cctgtataaa
    54601 aagtatggtt ttcgtattga aagtatgcga aaaaacgaca tcaaatcaaa aaatggagat
    54661 gacttagatg tttatcatat gcgtaaatct gtaagtcagt aagtgaatcg atgaaaattg
    54721 gaaaaaaaat agttttaatg ttcacagcta ttgtgttaac aactgtcttg gcattaggtg
    54781 tctatctaac tagtgcttat accttctcaa caggagaatt atcaaagacc tttaaagatt
    54841 tttcgacatc ttcaaagaaa agtgatgcca ttaaacaaac aagagctttt tctatcttgt
    54901 tgatgggtgt tgatacaggc tcttcagagc gtgcctccaa gtgggaagga aacagtgatt
    54961 cgatgatttt ggttacggtt aatccaaaga ccaagaaaac aactatgact agtttagaac
    55021 gagatacctt aaccacgtta tctggaccca aaaataatga aatgaatggt gttgaagcta
    55081 agcttaacgc tgcttatgca gcaggtggcg ctcagatggc tattatgacc gtgcaagatc
    55141 ttttgaatat caccattgat aactatgttc aaattaatat gcaaggcctt attgatcttg
    55201 tgaatgcagt tggagggatt acagttacaa atgagtttga ttttcctatc tcgattgctg
    55261 aaaacgaacc tgaatatcaa gctactgttg cgcctggaac acacaaaatt aacggtgaac
    55321 aagctttggt ttatgctcgt atgcgttatg atgatcctga gggagattat ggtcgacaaa
    55381 agcgtcaacg tgaagtcatt caaaaggtat tgaaaaaaat ccttgctctt gatagcatta
    55441 gctcttatcg gaagatttta tctgctgtaa gtagtaatat gcaaacgaat atcgaaatct
    55501 cttctcgcac tatccctagt ctattaggtt atcgtgacgc acttagaact attaaaactt
    55561 atcaactaaa aggagaagat gccactttat cagatggtgg atcataccaa attgttacct
    55621 ctaatcattt gttagaaatc caaaatcgta tccgaacaga attaggactt cataaggtta
    55681 gtcaattaaa aacaaatgct actgtttatg aaaatttgta tgggtcaact aagtctcaga
    55741 cagtaaacaa caactatgac tcttcaggcc aggctccatc ttattctgat agtcatagct
    55801 cttacgctaa ttattcaagt ggagtagata ctggctcatc ttatcagagt gctagtacag
    55861 accaggactc tactgcttca agccatacgc caactacgcc gtcttcttca tcagatgctt
    55921 tagcagctga tgagtctagc tcatcagggt ctggatcatt agttcctcct gctaatatca
    55981 accctcagac ctaacgttct gagggttttt atgtttaaaa tatccgattt tgaatataaa
    56041 aaagacaggt atcaaagatg atagctgtct gtgatttcaa ataaagtata tgagggagat
    56101 aatactatct ggaatggttg ttagttaaaa tgacggtttg gcaagtaggt gattcactag
    56161 aaattagctc actatcttct tgatatttgg ccatacgatt ttggatttca gtaaggtgtg
    56221 cttgtgtctc ttggttaaag tagcgccatt tatgggccaa gtcctgacta atattttgaa
    56281 ttacttttcc ttgactgtga atgaggtcaa ggtttgcttt aatgttatct aaatcaagtt
    56341 gggcagcatc ttttgcttgt cgactgatgg ataacagctc ttttatggta caacgttttt
    56401 gatatgcttt gtaagcggta aaagataata atccgacggc tatcacggtt ttaactttca
    56461 tgaactaacc tcctgattga tttgtcgggc gagcttttcc aagacaggaa agttaggctc
    56521 atgagtggtg tattgaattg agataccgtc tttttcatta taacgaggaa cgagatgaac
    56581 atgagcatgg aaaacagttt gtcccgctaa ggcttcgtta ttgttaatga tattcatggc
    56641 agtagcgcca gtagctaact ggatagcacg agcaatttta ggaatacgag caaagagatg
    56701 actagctgtc tcagcagtca tctctagtaa gttacgaaca tgttgcttag gaatgactag
    56761 agtatgccct tttgtagttt gtgagatatc taaaaaggcg aggacctgct catcttcgta
    56821 gactttcgaa gaggggatat ccccttgaat gatgctgcaa aaaatacaat tttccatttt
    56881 atatcacctt ctctacttga atttgttata atatattata agtatatcaa aaactagaaa
    56941 gaaattatat gttaaacatt aaaaatctaa caggaggata tcacaatatc cctgttttaa
    57001 acgatgtctc attttctgta gataatggag agttagtcgg tttgatcggg ctaaatggtg
    57061 ctggaaagtc aacaactatc aacgaaatta ttggcttttt gaagccttat caaggaagta
    57121 tttctattga tggtttaact ctagcagaaa atgcagttgc ctaccgtcaa aaaataggtt
    57181 ttatcccaga aactcctagt ctctatgaag aattgacctt atcagagcat atcaacaccg
    57241 tagctatggc ttatgatatc gacttagaag tggcacaaaa acgagcacaa cctttcttgg
    57301 aaatgtttcg tttaactgac aaacttgaat ggtttcctgt caatttttct aaagggatga
    57361 aacaaaaagt catgattatt tgtgcctttg tcattgatcc aagtctattt attcttgatg
    57421 aaccattctt agggttagac cctttagcta tttcagactt aatccaaacg ctagaagtcg
    57481 aaaaagcaaa aggaaaatct attttgatga gtacacatgt tttggactct gctgaaagaa
    57541 tgtgtgatcg ctttgtgatt ttgcatcatg gacaagttcg tgcccaagga actcttgctg
    57601 acttgcaaga agcttttggt gaccgttcag cgagcttaaa tgatatctat cttgcattaa
    57661 caaaagagga ttaagtatga aagcattatt tctgaaacgc cgtcaagact ttcaaaaaca
    57721 acaaaacaaa taccttcgtt acgtcttaaa tgatcacttt gttttggtgt tgatgttttt
    57781 attaggctat gctatggtgc agtatggtca gctactaaac cattttccaa cgaatcatct
    57841 acctattcag gtttgtcttg ggatattgat cccattattg ctcagtatgg gatctatagc
    57901 aacgtatcta gaagaggcag atcagcattt cttacttcca aaagaagaag aagtgatttc
    57961 ttatatcaaa caagctgaac gactttcttt tttgctttgg ggaactttgc agactgctat
    58021 attgctcttt ttatatccta tttttagacg tctaggtctg tctttattta tttttattat
    58081 cttagttttg atattattgg cactaaaaag agtagtgctt agtcgaaaga caagatattt
    58141 tttgagagga aatcgtttag attgggcaaa agctgtcgca tttgaaagca atcgcaagca
    58201 aagtatttta aagttttatt ctctttttac aacagtcaaa ggaatttcta caaaagtgaa
    58261 agaaagaact tatctaaatc ctcttttaaa attagttaag caaactcctt caaacctttg
    58321 gctatccctt tatgctagag cctttttacg tagttcagat tatttgggct tatttttacg
    58381 actaatgcta ttaagctcat tatctgtatt ttttatccat aatctttatt tgtcagtaag
    58441 tttagcactc atttttaact atttagttgt attccaatta ctttcacttt attatcacta
    58501 cgattaccat tatatgacta gtctttatcc tgaaaatagt cggagcaaga agaaaaatgt
    58561 gttaagtttt ttacgaggtt taagcttttt aatgttaatc gtcaatatgc tgtgttgctc
    58621 atcagcgcca aaagcactca ttttgattgt aggtatggtt ttcatagctt gtatatatct
    58681 accttataag ttaaagaaga tcattgacta agtagcctaa aacaagtaaa ataggggtaa
    58741 acgactataa aagatagaaa aggagggcga taaggtgaca acgacggaac aagaacttac
    58801 cttgactccc ttacgtggga aaagtggcaa agcttataaa ggcacttatc caaatgggga
    58861 atgtgtcttt ataaaattaa atacgacccc tattctacct gccttagcaa aagaacagat
    58921 tgcgccacag ttactttggg ccaaacgcat gggcaatggt gatatgatga gtgcccaaga
    58981 atggcttaac ggccgtacat tgaccaaaga agatatgaac agtaagcaaa tcattcatat
    59041 tctattgcgc cttcacaaat ctaaaaaatt agtcaatcaa ctgcttcagc tcaattataa
    59101 gattgaaaac ccatacgatt tattggttga ttttgagcaa aatgcaccct tgcaaattca
    59161 gcaaaattca tacttacaag ctatcgttaa agaattaaaa cggagcttac cagagttcaa
    59221 atcagaagta gcaacgattg tgcatggaga tattaaacat agcaattggg tgattactac
    59281 tagtggtatg atttttttag tagattggga ttctgttcgt ctgactgatc ggatgtatga
    59341 tgttgcttac ctgttgagcc actatattcc acggtctcgt tggtcagaat ggctgtctta
    59401 ttatggctat aaaaataatg acaaggttat gcaaaaaatt atttggtatg gtcaattttc
    59461 tcacctgaca caaattctca agtgttttga caagcgtgac atggagcatg tgaatcagga
    59521 gatttatgcc ctcagaaaat ttagagaaat atttagaaag aaataatgcg agttagaaaa
    59581 cgaaaaggtg ctgaggagca cttagctaat aacccacact atgtgatatt aaatccagaa
    59641 gatgccaagg gccgttggca tgacgttttt ggaaatgacc gtcctattca tatcgaagtt
    59701 ggttcaggaa agggcggatt tatcacaggg atggccttga aaaatcctga tattaactac
    59761 attggcattg atattcaatt atctgtccta agctacgcct tggataaggt attagcttca
    59821 gaagtaccca atgtcaaact tctgcgagtg gatggatcaa gtttaaccaa ctattttgaa
    59881 gatggtgagg tggatatgat gtatctcaac ttttcagatc cttggccaaa aaccaagcat
    59941 gaaaaacgtc gtttgaccta caaggatttc ttagacactt acaagcgtat cttgccagaa
    60001 cacggtgaaa ttcattttaa aaccgataat cggggcttgt ttgagtacag tttagctagt
    60061 ttttctcaat acggcatgac cttgagacag atctggcttg acttacacgc tagtaactat
    60121 gaaggaaatg tcatgacaga atatgaagag aagttctcta ataaaggtca agtcatttac
    60181 cgagttgagg caaatttcta gtagtatttt gtttcaaaat atgataacat aaaggagttg
    60241 atacagtatt aacttcttta gtaatggaga ggtcgcatag tggcttagtg cgcacgcttg
    60301 gaaagcgtgt agtccttaac ggggctcggg ggttcgaatc ccctcctctc cttgtgagta
    60361 aaacaaagtg catatccttg cattcctctt aaattatggt ataatgggct taaataatac
    60421 agaaaggtgg tgggatatgt ctcgccgctt ttgtattaag tggattccca aggaccaatt
    60481 atcttagaga agagcataaa aatagaggag gtaatcaaaa tagcaaatac atctattatc
    60541 gatatcgtaa ccaagacagt aacacctgag attaaggcac cttatgaatt agtagacgtt
    60601 gaatatgaca aaatgggtag tgactacatc cttagtattt tagtcgataa ggaaggtgga
    60661 atcacagtgg aagatacatc tgacttgacg aatattatta gtccgctgtt agatacgatt
    60721 gatccagatc cttttccaaa ccaatatatg ctagaagtct ctagtccagg tttggagcgt
    60781 ccgcttaaga cagctgacag tttaaaagca gcagttggct cttatatcaa tgtgagtctt
    60841 tatcaagcta ttgataaggt gaaagttttt caaggagact tactagcctt tgatggtgaa
    60901 acgttgacca ttgattatct tgataaaaca cgtcataaga tcgtgaatat accatatcaa
    60961 gcagttgcta aggtgagaat ggctgtcaaa ttataacttt tatttcaaag cactaaactt
    61021 ttttaggaac gcttaagcaa atttgaaaca acacctatag agtttataca agctaaacaa
    61081 aaggaattaa gggagccttt agggggctat tgatcgtatt aatttaagaa aggatttttg
    61141 ccaagcttta caagcaaggt agataacatt atgagcaaag aaatgctaga agccttccgt
    61201 attttggaag aagaaaaaca cattgataaa gcagatatta ttgacgcagt gacagaatca
    61261 ttaaagtctg cctataaacg tcgctatggt caatcagaat catgtgttat tgaatttaat
    61321 gaaaaaacag ctgattttca agtattcacg gttcgtgagg ttgttgaaga agtttttgac
    61381 agccggcttg agattagttt gaaagatgct cttgctatca gttctgccta tgaattgggt
    61441 gataagattc gctttgagga atctgtcaat gaatttggac gtgtcgcagc tcaatcagct
    61501 aagcagacta ttatggaaaa gatgcgccgc caaatgcgtg aagtgatgtt caatgaatac
    61561 aaagagcatg aaggtgaaat tatgacagga acagttgaac ggtttgatca acgttttatt
    61621 tatgtcaatc ttggttcctt agaagcacaa ttgtctcacc aagaccagat tccaggtgaa
    61681 actttcaaat cacatgaccg tattgacgtt tatgtctaca aagttgaaaa taatcctaaa
    61741 ggagttaacg tctttgttag tcgtagccat ccagaattca tcaaacgtat tatggagcaa
    61801 gaaattcctg aagtctttga tggtactgtt gaaatcatga gtgtctctcg tgaagcaggt
    61861 gaccgtacca aggttgctgt gcgcagtcat aatcctaacg ttgatgccat cggaacaatt
    61921 gttggacgtg gtggaagtaa tattaaaaag gtcatcagca agttccatcc aaaacgtgta
    61981 gatgctaaga cggggcttga aattccagtt gaagaaaata ttgacgtgat tcaatgggta
    62041 gatgatccag ctgagttcat ctacaatgcc attgctccgg ctgaagtaga catggtactt
    62101 tttgacgatg aagatcttaa acgtgctacg gtagtagttc ctgatagcaa attatcatta
    62161 gctatcgggc gtcgcggcca aaacgttcgt ttagcggcac atttgactgg ctaccgtatt
    62221 gatatcaaat cagctagcga atatgatcgt cttgaggcgg aaaaagaagc agcaacagtt
    62281 gtggaagagc cagttgttga cgatgttatg actgccgagg aagtgtaaga aaagaggtgt
    62341 ttgatgtcta aagtcaaaaa aataccttta cgaaaatcac ttgtttctgg tgaaattatt
    62401 gctaaacgag atcttcttag gattgtaaaa actaaggatg gtcaagtttt cattgatcca
    62461 actggtaaac aaaatggtcg tggtgcttat atcaaattag acaatcaaga ggccttaatg
    62521 gccaagaaaa aacaagtttt caacaggagt ttctcaatgg acattccaga aagtttttac
    62581 gatgacttga ttgcttatgt tgatcacaaa atcaaaagaa gagagttagg tcttgactaa
    62641 tttagaaaga ttatctagtc ttattggtct agctcaacgg gctggaaaag tgatttcagg
    62701 tgaagaacta gtagtaaaag ctattcaaca ccagcaggta atattagtat ttttagccaa
    62761 tgatgctggt cctaatgtga caaaaaaagt gacagataaa agtaattatt ataatgtaga
    62821 agtctccaca gtgttgaatg cactggaatt aagtgctgca ctaggtaaac caaggaaggt
    62881 tgctgccgtt gcagatgctg gattttcaaa gaaaatgagg actcttatgg aatagatgaa
    62941 taggaggaca caaattgtca aagaaaagat tacatgaaat tgccaaggaa attggtaaaa
    63001 gtagtaaaga ggtggttgaa cacgctaaat acttaggatt agatgtgaaa agccatgcat
    63061 caagtgttga agaagcggat gcgaaaaaga ttatttcaag tttttcaaag gcttctaaac
    63121 ctgatgtgac agcttctcaa acagtcaagc ccaaagaagt tgctcaaccc tctgtaacag
    63181 tggtcaaaga aacaggttct gagcatgtag agaaaacaca ggtctctaag ccaaagagtc
    63241 gtaattttaa agctgaaaga gaggctcgtg caaaggaaca ggctgctcga aaacaagcaa
    63301 atggctcttc tcatcgttct caagaacgtc gaggaggtta tcgccagccc aataatcatc
    63361 aaactaacga acaaggagat aagaggatta cccatcgttc tcaaggtgat acaaacgata
    63421 agcgtattga gcgaaaagca tcgaatgtat cacctagaca tgataatcat cagctagttg
    63481 gtgatcgtaa ccgttccttt gctaaggaga atcacaagaa tggtcgattc acaaaccaga
    63541 aaaagcaagg acgacaagag cctcaatcga aaagtcctaa aattgatttc aaagctcgtg
    63601 ctgctgcctt aaaagctgaa caaaatgctg aatactcacg ccaaagtgaa acacgttttc
    63661 gtgcacagca agaagctaag cgtttagctg agttggcaag acaagaagct aaagaagcgg
    63721 ctttaaaagc acaagctgag gagatgagcc atcgtgaagc agcactgaaa tctattgaag
    63781 aagcagaaac taaattaaag tcatcaaata ttagtgctaa atctactgct gataatcgcc
    63841 gtaaaaaaca agcacgtcct gaaaagaatc gtgagttaac tcatcattca caagaaggac
    63901 aaaagaaaaa taagaagagt tggaatagtc aaaaccaagt gagaaatcaa aaaaatagta
    63961 actggaataa aaataaaaag accaaaaaag gcaaaaatgt taagaatact aacaccgctc
    64021 caaaaccagt aaccgagcgt aaattccatg aattgccaaa agaatttgaa tatactgaag
    64081 gcatgacagt tgccgaaatc gcaaaacgta tcaaacgcga acccgctgaa attgttaaaa
    64141 agctgtttat gatgggtgtt atggcaactc aaaaccagtc tttagatggg gatactatcg
    64201 aattattgat ggtagattat ggtattgagg ctaaggctaa ggtagaagta gatgatgctg
    64261 atatcgagcg cttctttgag gatgaaaatt acctcaatcc tgaaaatatt gtagaacgcg
    64321 cacctgttgt aactatcatg ggacacgtag accacggtaa gacaactttg cttgatactt
    64381 tgcgtaactc acgtgtggca acaggagaag ctggaggaat tactcagcat attggtgctt
    64441 accagattga agaagctggc aagaaaatta ccttcttgga tactcccgga cacgcggcct
    64501 ttacaagcat gcgcgcacgt ggagcttccg ttaccgatat caccattttg attgttgcag
    64561 ctgatgatgg tgttatgcca caaacgattg aagcgatcaa tcactcaaaa gctgctggtg
    64621 ttccaattat tgttgctatc aacaaaattg ataaaccagg tgctaatcca gaacgtgtta
    64681 tcgcagaatt ggcagaatat ggtatcatct caactgcttg gggcggagag tgcgaatttg
    64741 tagagatatc tgctaaattt aataaaaata ttgatgaact attagaaaca gtccttttgg
    64801 ttgctgaagt agaagaattg aaagctgatc cgacggttcg tgctattggt actgtgattg
    64861 aagcacgctt agataaaggt aaaggtgcta tcgctacgct tcttgtccaa caaggaactc
    64921 ttcatgttca agacccaatc gttgttggta atacctttgg tcgtgtccgt gccatggtaa
    64981 atgaccttgg gcgtcgtgtt aaatctgcgg agccatcaac accagtttcc attactggtt
    65041 tgaacgaaac accaatggct ggtgatcact ttgctgttta cgctgatgaa aaagctgcgc
    65101 gtgcagctgg tgaggagcga tcaaaacgtg ccctcctcaa acaacgtcaa aatacacagc
    65161 gtgttagcct tgataatctc tttgataccc ttaaagcagg agaaatcaag actgttaatg
    65221 tcatcatcaa agcagacgtt caaggttctg ttgaagccct tgcagcatct cttgtaaaga
    65281 ttgaagtgga aggtgtccgt gtcaatgtcg ttcactctgc tgtaggtgct atcaatgagt
    65341 ctgatgtgac ccttgctgaa gcatcaaatg ctgttattat cggtttcaac gttcgcccta
    65401 ccccacaagc tcgtcaacaa gcagatacgg atgatgtgga aattcgtctt catagcatta
    65461 tctataaagt cattgaagaa gtagaagaag ccatgaaagg gaaacttgac cctgtttacc
    65521 aagaaaaaat tcttggtgaa gccattattc gtgaaacctt caaagtttct aaagtgggaa
    65581 caattggtgg ctttatggta atcaacggta aggtgacacg tgattcgagt gttcgtgtta
    65641 tccgtgatag tgtcgttatt tttgatggta aactggccag cttgaaacat tacaaagatg
    65701 acgttaaaga agtcggtaat gctcaagaag gtgggttgat gattgaaaac tttaacgatc
    65761 ttaaagttga cgatactatt gaagcctata ttatggaaga aattgtgaga aaataggggc
    65821 taagaaggtc tgagatgtga cgtttttcct ctgtcggaaa taatgacttg ttctaccaga
    65881 tgagctactt ggggcattcc ttccagtctc gtcatggaat tctcagattc cctttagttg
    65941 tcgttatcaa aacaggtagc aatgtcataa gctgggcttt cagcctagct tttgttttaa
    66001 aaatgtggaa aggaaatggc tatggctaat catcgtattg accgcgttgg gatggaaatc
    66061 aagcgcgaag taaatgatat tcttcagaaa aaagtgcgtg atccacgcgt tcagggagta
    66121 acgattacag aagtgcaaat gcagggtgac ttgtcacttg ctaaagttta ttacactatt
    66181 atgagtgact tggcatctga taatcaaaaa gcacaaacag ggcttgaaaa agcgactgga
    66241 acgattaaac gtgaacttgg caaacaattg accatgtaca aaattccaga tttggtgttt
    66301 gaaaaagaca attccatcgc ctatggtaat aaaatagatc aactgttacg tgagcttgat
    66361 aaaaaagatt aaattaaaaa cctttaaggt atcttattag agtcacctta agggtttttt
    66421 gtttttagta agctttttga atcgtatcaa tgatttgata ttgtgcagtc ttggcttctt
    66481 caattggata gattgggtaa acatgattca ttttatcagc gacgataagt tcatgttgat
    66541 gacctttggc agttaatata tcatcaaatt tcttaacatc tgggtagaaa atttcatgtg
    66601 taccaacaaa catagtaata ggtgctagcc ctgatacatc tccattaatt ggactaacat
    66661 atggatgttt ggtgttgttt gggtcacggc gtgcccaaat ttccccaact ttacgtaggc
    66721 cccaagctga aagaatggga tcattacttt catagctctc aatatctgga tttgacatgg
    66781 ttacatctaa ccatggtgat aagagaacaa tgtcttttgg ttgttttaaa ctttcatttt
    66841 ttagggctaa tgctaaacca agcgctagtc caccacctgc tgaatcaccc ataatagtga
    66901 tattttgtgc agttgtttct ttaagagtat ctctgtaaag gtcaaccaat tttggcatag
    66961 catcactgta agagtaagta ggtgcttttg gatagattgg taagataatc ttagcatcaa
    67021 gactagttga taaggttttt agcatgtcga tatgataagt agttgggttg tttaggtaag
    67081 agcccccatg taaatagaaa atgacctttt gattggcttt attgctgggg ttctcattcc
    67141 aagtatgaac ttgcatatta tcaacagttt tagtgactaa tgtgccattt acctgttctt
    67201 ctttaagatc aaagccttta tttgaagctt ctccagcaga gttgacatat tgatcaaact
    67261 tagtttcgtc ttgctctgaa taatagcctt tgtctccttt taaccaaaag tatttctcta
    67321 taaaccagct tttccaagat ctagaactgg catcaacttg agtgctagct cctattgcta
    67381 gagtaagtcc tagtaaaata agagtgcttt taagtgtctt tttcaatga
//
LOCUS       DATUYR010000003        61671 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.003, whole genome shotgun sequence.
ACCESSION   DATUYR010000003 DATUYR010000000
VERSION     DATUYR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61671
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.003"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            101..2299
                     /gene="nrdD"
                     /locus_tag="VCI33_000143"
     CDS             101..2299
                     /gene="nrdD"
                     /locus_tag="VCI33_000143"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEP2831101.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIIKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            2481..2627
                     /locus_tag="VCI33_000144"
     CDS             2481..2627
                     /locus_tag="VCI33_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831102.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            2656..3591
                     /locus_tag="VCI33_000145"
     CDS             2656..3591
                     /locus_tag="VCI33_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP2831103.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKASLDGVFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLSEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            3600..4109
                     /locus_tag="VCI33_000146"
     CDS             3600..4109
                     /locus_tag="VCI33_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2831104.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSYAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEEQRGDK"
     gene            4109..4723
                     /gene="nrdG"
                     /locus_tag="VCI33_000147"
     CDS             4109..4723
                     /gene="nrdG"
                     /locus_tag="VCI33_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEP2831105.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(4897..5628)
                     /gene="yaaA"
                     /locus_tag="VCI33_000148"
     CDS             complement(4897..5628)
                     /gene="yaaA"
                     /locus_tag="VCI33_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEP2831106.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQNLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(5670..6527)
                     /locus_tag="VCI33_000149"
     CDS             complement(5670..6527)
                     /locus_tag="VCI33_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP2831107.1"
                     /translation="MTYPYNSTISLGTVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTSFLVPDRHSLGLVLNHFLTRGISLEGSAD
                     HGYSEAIYLSGPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKKARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
     gene            6714..7051
                     /locus_tag="VCI33_000150"
                     /pseudo
     CDS             6714..7051
                     /locus_tag="VCI33_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(7330..8043)
                     /gene="treR"
                     /locus_tag="VCI33_000151"
     CDS             complement(7330..8043)
                     /gene="treR"
                     /locus_tag="VCI33_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEP2831108.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            8254..10278
                     /gene="treP"
                     /locus_tag="VCI33_000152"
     CDS             8254..10278
                     /gene="treP"
                     /locus_tag="VCI33_000152"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEP2831109.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QQVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGTVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMVVAIVVPMFLAFFFRKSHIMTKTEDEAKLPETPVSD
                     APVATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGSLPRQIKANDKLAVAMKK"
     gene            10346..11974
                     /gene="treC"
                     /locus_tag="VCI33_000153"
     CDS             10346..11974
                     /gene="treC"
                     /locus_tag="VCI33_000153"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEP2831110.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYAGEVELELPPR
                     YQHGQALISNYEKVSICEKVMLKPYQTLAILADN"
     gene            12182..14077
                     /gene="pepO"
                     /locus_tag="VCI33_000154"
     CDS             12182..14077
                     /gene="pepO"
                     /locus_tag="VCI33_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEP2831111.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGDHIPDDAILKNFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(14243..15283)
                     /locus_tag="VCI33_000155"
     CDS             complement(14243..15283)
                     /locus_tag="VCI33_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEP2831112.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(15417..16184)
                     /gene="rpsB"
                     /locus_tag="VCI33_000156"
     CDS             complement(15417..16184)
                     /gene="rpsB"
                     /locus_tag="VCI33_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEP2831113.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADIQADSIEEIVEVVEGDNA"
     gene            16488..19562
                     /locus_tag="VCI33_000157"
     CDS             16488..19562
                     /locus_tag="VCI33_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2831114.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLKKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYINSQLGSQPVTEEEMEHLYQASQGNPFFLSEYTQTLLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDRIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKNIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVAY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLQIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADYLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(19593..20579)
                     /locus_tag="VCI33_000158"
     CDS             complement(19593..20579)
                     /locus_tag="VCI33_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEP2831115.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHN"
     gene            complement(20765..22306)
                     /gene="hutH"
                     /locus_tag="VCI33_000159"
     CDS             complement(20765..22306)
                     /gene="hutH"
                     /locus_tag="VCI33_000159"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEP2831116.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGVACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKRVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLIEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KPENHELGKGTKVAYDLFRKEINFIEHDKHIEIYDELNKASAVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(22317..23660)
                     /locus_tag="VCI33_000160"
     CDS             complement(22317..23660)
                     /locus_tag="VCI33_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEP2831117.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRAPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVVFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(23880..24476)
                     /locus_tag="VCI33_000161"
     CDS             complement(23880..24476)
                     /locus_tag="VCI33_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEP2831118.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDINLMYDDQYQGQLLPVWSDTPFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCPNQTSPAQTPVAVWAGLWYKT"
     gene            complement(24498..26171)
                     /locus_tag="VCI33_000162"
     CDS             complement(24498..26171)
                     /locus_tag="VCI33_000162"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP2831119.1"
                     /translation="MVLSDIEIANSVTMEPISKVANQLGIDEEALCLYGKYKAKIDAR
                     QLVALKDKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKANLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVALAEQDNQFCFVYEEDDSIETKLT
                     KIVTKVYGGKGIRLTPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFT
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(26189..26815)
                     /locus_tag="VCI33_000163"
     CDS             complement(26189..26815)
                     /locus_tag="VCI33_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEP2831120.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQAIITEIHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     SAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(26826..27725)
                     /gene="ftcD"
                     /locus_tag="VCI33_000164"
     CDS             complement(26826..27725)
                     /gene="ftcD"
                     /locus_tag="VCI33_000164"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEP2831121.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELDIPIFLYEDSATRPERQNLAKIRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEVIGLAPAKALIDV
                     AEYYLQVEDFDYPKQVLENHLLG"
     gene            complement(27813..29843)
                     /locus_tag="VCI33_000165"
     CDS             complement(27813..29843)
                     /locus_tag="VCI33_000165"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEP2831122.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRWRPKDRIYGKPINEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFQSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVTQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTVALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            30135..31400
                     /locus_tag="VCI33_000166"
     CDS             30135..31400
                     /locus_tag="VCI33_000166"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEP2831123.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(31458..32990)
                     /gene="ahpF"
                     /locus_tag="VCI33_000167"
     CDS             complement(31458..32990)
                     /gene="ahpF"
                     /locus_tag="VCI33_000167"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEP2831124.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVATKDIVGEDHVTGLSYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(33011..33571)
                     /gene="ahpC"
                     /locus_tag="VCI33_000168"
     CDS             complement(33011..33571)
                     /gene="ahpC"
                     /locus_tag="VCI33_000168"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEP2831125.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            33978..34048
                     /locus_tag="VCI33_000169"
     tRNA            33978..34048
                     /locus_tag="VCI33_000169"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34010..34012,aa:Cys,seq:gca)
     gene            34567..34770
                     /locus_tag="VCI33_000170"
     CDS             34567..34770
                     /locus_tag="VCI33_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEP2831126.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            34966..35427
                     /locus_tag="VCI33_000171"
     CDS             34966..35427
                     /locus_tag="VCI33_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEP2831127.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            35427..37871
                     /locus_tag="VCI33_000172"
     CDS             35427..37871
                     /locus_tag="VCI33_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP2831128.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSYGKLTFHIA"
     gene            38049..38339
                     /gene="groES"
                     /locus_tag="VCI33_000173"
     CDS             38049..38339
                     /gene="groES"
                     /locus_tag="VCI33_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEP2831129.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            38375..40006
                     /gene="groL"
                     /locus_tag="VCI33_000174"
     CDS             38375..40006
                     /gene="groL"
                     /locus_tag="VCI33_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEP2831130.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            40257..40523
                     /locus_tag="VCI33_000175"
     CDS             40257..40523
                     /locus_tag="VCI33_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2831131.1"
                     /translation="MNHFGEIFKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMSIDEFMYAVHDFHRDDLNELLSKSEGFRNNSR"
     gene            complement(41246..42745)
                     /locus_tag="VCI33_000176"
     CDS             complement(41246..42745)
                     /locus_tag="VCI33_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP2831132.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDPSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVTKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVEEKPQNKKGLNRS"
     gene            42992..44632
                     /locus_tag="VCI33_000177"
     CDS             42992..44632
                     /locus_tag="VCI33_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831133.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDRKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALAPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(44721..45722)
                     /locus_tag="VCI33_000178"
     CDS             complement(44721..45722)
                     /locus_tag="VCI33_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP2831134.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTQEDICVQAKSATFDAVLNAQKARK"
     gene            46299..46679
                     /locus_tag="VCI33_000179"
     CDS             46299..46679
                     /locus_tag="VCI33_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP2831135.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            46718..49054
                     /locus_tag="VCI33_000180"
     CDS             46718..49054
                     /locus_tag="VCI33_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP2831136.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            49103..49255
                     /gene="rpmG"
                     /locus_tag="VCI33_000181"
     CDS             49103..49255
                     /gene="rpmG"
                     /locus_tag="VCI33_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP2831137.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            49269..49445
                     /gene="secE"
                     /locus_tag="VCI33_000182"
     CDS             49269..49445
                     /gene="secE"
                     /locus_tag="VCI33_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEP2831138.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(49682..50455)
                     /locus_tag="VCI33_000183"
     CDS             complement(49682..50455)
                     /locus_tag="VCI33_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEP2831139.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            50581..51327
                     /locus_tag="VCI33_000184"
     CDS             50581..51327
                     /locus_tag="VCI33_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP2831140.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLEVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKIITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSSGGLVLDFVS"
     gene            51343..52323
                     /locus_tag="VCI33_000185"
     CDS             51343..52323
                     /locus_tag="VCI33_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEP2831141.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRKQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            52611..52931
                     /locus_tag="VCI33_000186"
     CDS             52611..52931
                     /locus_tag="VCI33_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP2831142.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            52959..53267
                     /locus_tag="VCI33_000187"
     CDS             52959..53267
                     /locus_tag="VCI33_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2831143.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            53277..54581
                     /locus_tag="VCI33_000188"
     CDS             53277..54581
                     /locus_tag="VCI33_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP2831144.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMELISCFAIGYSYAKNSDVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGIIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            54792..57209
                     /locus_tag="VCI33_000189"
     CDS             54792..57209
                     /locus_tag="VCI33_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEP2831145.1"
                     /translation="MIETKSPYFGHLTDRMAHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWHALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            57222..57890
                     /locus_tag="VCI33_000190"
     CDS             57222..57890
                     /locus_tag="VCI33_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP2831146.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     DSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            57947..59035
                     /locus_tag="VCI33_000191"
     CDS             57947..59035
                     /locus_tag="VCI33_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEP2831147.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGNSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(59117..59626)
                     /locus_tag="VCI33_000192"
     CDS             complement(59117..59626)
                     /locus_tag="VCI33_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEP2831148.1"
                     /translation="MSTDHHLREVSYQLLAERGVSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGNSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            59990..60805
                     /gene="sdaB"
                     /locus_tag="VCI33_000193"
     CDS             59990..60805
                     /gene="sdaB"
                     /locus_tag="VCI33_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEP2831149.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(61046..>61671)
                     /gene="ropB"
                     /locus_tag="VCI33_000194"
                     /gene_synonym="rgg1"
     CDS             complement(61046..>61671)
                     /gene="ropB"
                     /locus_tag="VCI33_000194"
                     /gene_synonym="rgg1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="RopB (Regulator of Protease B) is a paralog of the
                     quorum-sensing transcriptional regulators Rgg2 and Rgg3,
                     which bind short, hydrophobic peptides encoded by genes in
                     their respective vicinities. RopB binds the leaderless
                     short peptide SIP (SpeB-inducing peptide), with sequence
                     MWLLLLFL; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     RopB/Rgg1"
                     /protein_id="HEP2831150.1"
                     /translation="IDMDTAKHYFECRNIEGLNHILDSYKDSKSTKEKNLFALVKVLL
                     ATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESCFIEMVFSKVILNLDK
                     YNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQLNDDCLYERCCVSFFD
                     GIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIKHKLS"
BASE COUNT        18429 a        11795 c        13387 g        18060 t
ORIGIN      
        1 gtaatctaat atcaatatat tgtgttcgag caaaaaactt gacacaatat attgattttt
       61 ttagttgtcg tgatataata gttccgtaag gagatttgat atggtaagtt tagaagaaga
      121 caaggtgact gttcaacctg atattaaagt gattaaacga gatggtcgcc ttgttaattt
      181 tgatagtaca aaaatctata gtgctttatt aaaagcaagc atgaaagtaa ctcggatgtc
      241 gccacttgtt gaggctaaat tagaggctat ttctgatcgc attatagcag aaattattga
      301 gcgttttcca actaatatca aaatttatga aatccaaaat attgtagagc ataagcttct
      361 tgcagctaat gaatatgcta ttgcaaaaga atacattaat tatcgtactc agcgtgactt
      421 tgcacgttca caagcaacag atatcaattt ttctattgat aaattaatta ataaagatca
      481 aacagttgtt aatgaaaatg ctaacaaaga tagcgatgtt tttaatactc aacgagattt
      541 aactgctgga atcgtaggga aatcgattgg tttaaaaatg ttaccttcgc atgttgctaa
      601 tgctcatcaa aaaggagata tccattacca tgatttggat tacagtcctt atacaccgat
      661 gacgaactgc tgtttaattg actttaaggg catgttagcc aatggcttta aaattggtaa
      721 tgctgaagtg gaaagtccca agtctattca aactgcaaca gctcagatct cacagattat
      781 tgcgaatgta gcatcaagtc agtacggcgg atgcacagct gatcgcattg acgagttttt
      841 agccccatat gcggagctta actttaaaaa acatatggct gatgctaaga aatggatcgt
      901 tgagactaag agagaaagct atgcttttga aaagactcaa aaagatattt atgatgcgat
      961 gcagtctttg gagtatgaaa ttaatacgct ctttacgtct aatggtcaaa caccatttac
     1021 ttctttagga tttggtttgg ggacgtcttg gtttgaacgt gagattcaaa aagctatttt
     1081 gaccattcgg attaatggtc ttggtagtga acatcgcacg gctattttcc ctaaattaat
     1141 tttcacggtt aaacgtggct tgaatttaga accagattca ccaaactatg atattaagac
     1201 tttggcttta gaatgtgcga ctaagcggat gtacccggat atgttatctt atgataaaat
     1261 tattgatttg acaggatctt tcaaatctcc aatgggatgc cgctctttcc ttcaaggctg
     1321 gaaagatgaa aatgggcaag atgtgacctc aggccgtatg aatcttgggg ttgtcaccct
     1381 caatttacct cgcattgcca tggaatcaaa tggcgatatg gataagtttt gggagctgtt
     1441 taatgagagg atgctaatta gtaaggatgc tttaatttat cgtgtcgaac gtgtcacaga
     1501 agcaaaacca gcaaatgccc ctattcttta tcaatatggt gcttttggaa agcgtttgga
     1561 gaagacaggg aatgtaaatg atctctttaa gaatcgtcgt gcaacagtct ctcttggcta
     1621 tattggtctt tatgaagtgg cgtctgtttt ttatggtggt caatgggaag gtaatccaga
     1681 tgctaaagct tttaccttgt caattatcaa ggcaatgaaa caggcctgtg aggattggtc
     1741 agatgaatat ggttatcatt tctctgttta ttcgactcca tcagaaagtt tgacagatcg
     1801 cttttgtcgt ttagatacgg aaaaatttgg cattgtgaca gatattacgg ataaagaata
     1861 ctatacaaat tcttttcact atgatgtgcg taagagtccg acaccttttg aaaaattaga
     1921 ttttgaaaaa gattatccag aagcaggtgc ttcaggtggt tttatccact actgtgagta
     1981 tcctgttttg caacaaaatc caaaggcctt ggaagcggtt tgggactatg cttatgatcg
     2041 tgtggggtat ttgggaacca atacgcctat tgataaatgc tataattgcc aatttgaagg
     2101 cgattttacc ccaacagaac gtggttttac ttgcccaaac tgtggcaata atgaccctaa
     2161 aacagttgat gtggtcaaac gtacatgtgg ttacttgggg aatcctcagg cccgcccaat
     2221 ggttaacggt cgccataagg aaatctctgc gcgtgtaaaa catatgaatg gttctactat
     2281 aaaataccca ggcctgtaaa ctcaagctgg gggagagccc ttttgatgga aatatgatat
     2341 gcttaaaagg ttgcttcatg caagcaatct tttattgttt aatggtttct tagcaattgt
     2401 gagataagaa taactaatcc aaaacgacta attagctgtt agcaatcgga ctataagaag
     2461 aaagagataa ggaagaaata atgggaaaat atcaattaga ctataaagga atgcagcagg
     2521 tagaacgctt tcatgagaag cattctaaaa aaaagacgga taaaaaatcc cgcgttcaag
     2581 aacttaaggc tcggttttta gagaagtcaa aaaaacagga taactaaagt atccaataaa
     2641 gaaagaaggt tgagtatgct taatattggg attgtgggac taggtgccat ttcacaaaaa
     2701 gcctatttac catatatgag acagctgagt gacattactt ggcatctatc aacgcggaat
     2761 gcagcagtac gtcagcaggt cggtcagtta tttggtcacg ctattcttta cagtgatgtc
     2821 aaggagttgt caaaggcaag tttagatggc gtctttattc atgcggccac atcggcccac
     2881 gcagagttgg ctagtttatt tttaaatcag gggatcccag tctttatgga taaacctata
     2941 gctgacaatt acctgatgac aaagaacctc tatgacttag ccaaagaaaa tcagactttt
     3001 ctgatggcag gatttaacag acgttttaca cctcgtgtca agaagttgtc aagtttgtca
     3061 actaagcgta aagtggctgt tgaaaaaaat gacttgaacc ggccaggaga tatgactttt
     3121 aagctctttg atttctttat tcatccttta gatacagcct tattcttgtc agaagggacc
     3181 ttactaaaag ggcattttca atatcatctg gaagcaggct tactgagtca agttatggta
     3241 accttgatga ccgaaagcat gacgactaca gcttctatga atttacaatc agggagtcgc
     3301 cgtgaggtaa tggaagttca acgggctgaa gaaacttatc atttagagaa cttagacgag
     3361 ttatctattt ataaaggtac tgaaaaaagg gtgcttggct ttgcttcttg ggacactacc
     3421 ttgcacaaaa gaggttttga aacaatgatc gatgcctttt tagaagctat tagcacaggc
     3481 gtcaatcctg tcagtcctga gtctagtctt ttaagccact ggatttgtca acagattgct
     3541 gactctcagc tttcttatgg agagttaacg gtagagttgc ctaaggatta ggaattgata
     3601 tggaaataag acgaccgact ttaaaagata aagacgctgt attgtcaatg attaacgaat
     3661 ttttagagca gaaaagtgct acagatgggt tatggcattt taacgtcaat gactttaact
     3721 atgaaacatg gttggaagat tccttgcgac aggaaatggg tttgtccagt caaggagtgc
     3781 ctgctattca atacgtcgca tttgatgaga gaagtcaggc tatcgggttc ttaaatcttc
     3841 gtttacggtt aaatgagaga ttacttgaaa aaggtgggca tattggttac tctgtccgtc
     3901 ccagccagcg tgggaaaggt tatgccaagg agatgcttaa acaagctgtc agctatgcga
     3961 tttccaagaa tatcacgaca attttggtaa cttgtgatga gactaatgtt gcaagccgag
     4021 cagttattgt ggctaacggt ggtgtcttgg aagatagtcg aggtggaacg gaacgttatt
     4081 ggattgaaga acaaagaggc gataagtgat ggcagaaaag tgttggaata atcctaagcc
     4141 aaaggaatgg caggcagagg agttaagtca aggccggatt attgactata aggcttttaa
     4201 ctttgttgat ggtgaaggag ttcgtaattc cctttatgtg tctggttgtt tattccattg
     4261 caaggggtgt tataatgctg cgacttggtc ttttaaggcg ggaatgcctt atacgcaaga
     4321 acttgaagag cagataatga cggatttggc acagccttat gttcagggac tgacactttt
     4381 aggtggagaa ccctttttga atacgggtat cttaattcct ctgattaagc gtattcggcg
     4441 tgaattgcca gaaaaagata tttggtcttg gacgggttat acttgggaag aaatgatgct
     4501 tgaaacacca gacaaactgg aaatgctgtc cttaattgat attctggtag atggtcgttt
     4561 tgatatcaca aaaaagaacc tcatgttgca atttagaggc tcttctaacc agcgaattat
     4621 cgatgttcaa aagtctttgg ctgctaaaga agtgattatc tgggataagt taaacgatgg
     4681 ggaccaaacc tttgaacaaa ttagtcggga ggatttgctt taagaagctg acttatccac
     4741 atgatttttt tgaaaaggtc atgtgaaaac aggtccttta agggtgaatt ggggagaaag
     4801 taattgttat ttcctggttt tccccacatt atcctcatag ttatccacag gttgtgtata
     4861 agttgtagaa ttgtgaataa agactaaaaa agaggcctag gcctcttttt tgatatagac
     4921 aaattcatga tctgttgata ggtctggatg gtagtaaaaa ccagcaaaga caaggctttt
     4981 aaggctgtca acagtttggc aattattttc catacaggca gtgagaaatg cacctcgtgc
     5041 cttttttgaa atggtggaat gggttttgaa ttggccctct ttttctgcca taaacttggg
     5101 acttatccac aattgtttac agtctttgga aaagacatcg tcaaattcac tggataaaag
     5161 ggaaatcact tggggatgct ctttggcaaa ctgattataa cagggccgcc agtaagactt
     5221 gaggctttgt ccttcaattt ttattctggt gtgaaaatca tggcggtgct ctgcgatagg
     5281 atgattagcg ggaatgatac cgtaaaagga tgaggtgatg taaacttgct gggtgaggta
     5341 agcctgttct tgggtagtga gcttatcgcg cttgatatgg cggtacataa ggccattgaa
     5401 cagttggtag gcagggtaag ccaagttttg ttgagaggcc atgtcttgcc agcgttgctg
     5461 ttctttttta gctgcctctt ctttgatacg ataggatttg gccaagtcct ccgtggtcat
     5521 agctgccatg atttttaaaa tggcttggct gtcttgtgga aggaggtgtg ggtgagattc
     5581 cttagggatc atcatttctt ttgcggttgg aattaaaaag gttagcatag tgttattgta
     5641 gtaaagttac ttgctttctg tcaagaaaac tagtcttgtc caaggatgac gttcacacag
     5701 ataccttcct cgtcttctgt cgtgaaagaa gaagaatctt cttgtaaaat agctaggccg
     5761 tgtaatcttg cttttttcag agtagctgaa aaaagcaggg gcgtctcaat gtgaatggtc
     5821 aagaaggcta agcctggggc accttcttgg tgttttttga ggtaaggggc tgaccagtga
     5881 ttgaatgcaa ggtgatgatg gtagttccct gaagcaatcc agctagcact tggaatggtc
     5941 attttatcgc ctaaatcaaa aaccttttga tagagaagtg aagaagccag agcatttttg
     6001 acactcagat ggacatggcc aatgcgtgtg tcttgagcta gtaaaaagcg cttggggata
     6061 tctgtcaact gctccaatat gcttttggta tccgtgggct cagtcacacc aataatttga
     6121 ccattgtcgc ggatatccca atgctcaaca gctttgtcat gataaatttc aatcccattg
     6181 ccctcaggac cgctgaggta gatagcttcg ctatagccgt gatcagctga tccttctaaa
     6241 gagatacctc tggttaagaa atgattgaga acaagtccca agctatgacg atcaggaact
     6301 aaaaaggatg tgtgataaag accataagcc ttatttgctg gtaaaggtgt ttgtcgtaac
     6361 tctaagataa cggttttacc gtctgtagtc aattgacgag atgtcgtgtc ttgtgagaga
     6421 acctgtaaac cgataatact tgtgtaaaag gttgtcatct ttgctagatc ggtaacattt
     6481 agagaaaccg ttcctaaaga gatggttgag ttgtaagggt atgtcatcct attacctcca
     6541 aaaactaatt aaatctatca taatcaatca gtttcaaaaa gaaaagtagg cacaaataag
     6601 tggtataggc actaaaaagt aactcatttg tcagccctat cttgtttctg ttctctctaa
     6661 tagtctataa tgggttataa gttacaaaaa gatactaaga ggagctgctg cctatgctga
     6721 aaaaaagacc ggcttgtccc gtagaaacaa ccttatcggt aatcgggaat aaatagaaat
     6781 tattaatatt aagggacttg ctaaagggac cttgcgtttt gggcagttga agtcctcaat
     6841 aggtttggtc agtcaaaagg tacttactgc acaattaagg gctatggaag cagacggatt
     6901 ggttcatcgg gaagtgtatg cagaagtgcc acctcgggtg gagtattcct taacagaaac
     6961 tgggttgagc ttagctcctg ttattgaagc gatgtcggat tggggccaaa cttaccaaga
     7021 aaaacatgcc gatttatttg aagaaggcta gaggaaagag ttgtccgagc atactgagac
     7081 ttagagaagc tgatagtttt taaaaaatga tattatcaaa ttctttacag tttgataaaa
     7141 ttactttttt tctacaaaaa gacttgcgct catctcacga atatgataat ataatatcaa
     7201 gtatgtttga gaaaacagaa gctcaggtca agtaagtgca cacttacaat aacacagttg
     7261 ataatgggga aactcagagt caagaaaaag aaaagcaagc cttgcatttt gcaaggcttt
     7321 tttctgtctt tattttggtc tgcgccgagc aaaatctaaa aatttaaatt tttccagttt
     7381 atggcgactc tcggtgaatt ggaattggtt attattggaa aggtagactt tggatttgac
     7441 ggaaacaaca tgttggtctg atccaaggtc aagtaaaatt ttatcacgat cggttatttg
     7501 gtcaatggta acctctttga gggcaaagtc aatggcgaga tggagttcct tttccaaata
     7561 ttgataaata gaatgctctg cgatttccct tgtcattatt ggcaccaaag ttttactaag
     7621 gtaatcaatg tctaagacgg aagccacacc ctcaacaacg cgttggcgtg taacacgcca
     7681 gaccaagctg tttttgctaa aaccggtcaa ttttgaaagt gtctcatcca caatcagttt
     7741 atcaatggca atgacgttgg tcttagaatc tagatttgag taagagacga gttcctgata
     7801 actagttagt tctgagatag ggaacatgat ctgatgatgt ttaatcactt gggtgccacg
     7861 tccttgtttt ttgaggataa gacctgcctt tgtgagaagt gagagagcct tgcggacggt
     7921 atctcggcta gcttggtatt gttgactcaa ttctatttca gtaggtaaaa aatccccttc
     7981 tttgtaaaag tctttgttta tttttgtttc gaggtcttta taaatacgtt catactttgt
     8041 catagggtaa attacctttc catttgtcta tttttttaca gacaacacct tttattttac
     8101 caaaataatg gcttttttgg cacaaacaac ttgtagacaa atttgcaatc gattacaata
     8161 aaggtgtcag ttagattcta tctaagaatt tgaatatttc attttgacgt tgcttgttaa
     8221 aagcaactag aacaaaggac taggggatgt cacatgggaa aatttgaaca ggatgctaag
     8281 agtcttctaa ctgctattgg cggtaaagaa aacatcaagg ctgtcacaca ctgtgcaacg
     8341 cgtatgcgtt ttgttttgaa tgataataat aaggcaaatg tcaaagagat tgaaaaaatc
     8401 tctgtagtta aagggacatt taccaatgct gggcagtttc aggtaatcat tggtaatgat
     8461 gttccagttt tttataatga ctttacagct gtttctggta ttgaaggggt gtctaaagaa
     8521 gctgccaaat cagcagctaa aagtaatcaa aatgccttac aacaggtgat gaccatgttg
     8581 gctgagattt tcacacctat tattccggcg attattgttg gggggcttat tttaggtttc
     8641 cgtaatattt tggagagtgt gccttttgaa tttcttgggc agcaggtcga aaaagggaaa
     8701 ttagtttttg atgcagctgg ggatcctgtt tggaatacga ttgtgagggt atctcctttc
     8761 tggtcagggg ttaaccattt cttgtggtta ccaggggaag ctattttcca cttcttacca
     8821 gttgggatta cttggtctgt gacgcgtaag atgggaacca ctcaaatttt agggactgtc
     8881 cttggtatct gtttggtgtc accacaatta ttgaatgctt atgcggtagc aggaacgcct
     8941 gctgctgaga ttgccaaaaa ctgggtttgg gattttggtt tctttaccat taatcgtatt
     9001 gggtatcagg cacaggttat tccagccctt ttagctggtc tgtcccttgc ttatcttgaa
     9061 attttctggc gtaaacggat tccagaagtg gtctcaatga tttttgtgcc attcctttct
     9121 ttgattccag ctttgatttt agcgcatacg gtattggggc caatcggttg gactattggt
     9181 aaagggattt cctttgttgt gttagctggt ttgactggtc ctgttaaatg gctattcggt
     9241 gctatctttg gtgccttgta tgctccgcta gttattactg gtttacatca catgacaaat
     9301 gccattgata cccaattaat tgctgatact gcaactcgta caactggttt gtggccaatg
     9361 attgctcttt caaatatcgc tcaagggtca gccgtttttg cttattattt aatgaatcgt
     9421 catgaagaac gtgaggctga aatatcgctt cctgcagcaa tttctgctta ccttggggta
     9481 actgagcctg ccttatttgg ggttaatgtt aaatacgttt atccctttgt agccggaatg
     9541 atcggatcag gtattgcggg tctcttatca acaaccttta atgttcaggc aaattctatt
     9601 ggtgttggtg gcttaccagg tttcatggct atcaatgtga agtacatgat tccattcttc
     9661 atctgtatgg tagtagccat tgtggtgccg atgtttttag ccttcttttt ccgtaaatca
     9721 catatcatga ctaagacaga agatgaagct aaactacctg agacacccgt ttcggatgct
     9781 cctgtagcaa ctgctccaca taagactatg caaggaacag ttatcacttt aacaagccct
     9841 ttaacgggtg aagttaaagc gttgtctgaa gctgttgatc ctgtctttgc acagggagtt
     9901 atgggccaag gtgctcttct tcaaccgaca gaaggggtgt tagtagcgcc ttgtgatgct
     9961 gaagtatcgg tcttgttccc aactaaacac gctatttgtt tggtgacgac tgaaggtttg
    10021 gaattattga tgcatattgg catggatacg gttaacttag atggtcaagg atttgaagct
    10081 ttggtgaagc aaggtgatca ggttaaggct ggacaaacat tgattcaatt tgatatagca
    10141 gctatttctg aagctggata cgccactgaa acgcctcttg tggtgactaa tcaagatgct
    10201 tttacggtaa ctgttgaagg tagtttaccg cgtcagatta aggctaatga taagttagca
    10261 gtagcgatga aaaagtaggt ttactaggga gaaaacgttc aggaactgag atcagttcct
    10321 gaaactattt tagggaggaa aactcatgac aattgataaa aagaaagtcg tctatcaaat
    10381 ttacccaaaa tcctataagg acacgactgg aaatggtgtg ggagacttgc gagggatcat
    10441 tgataaattg ccttacttac aagaactagg aatagatatg atttggttga accctttcta
    10501 ccctagtcca caacgagaca atggctatga tgtttcagat tatacggcgg tcaaccctga
    10561 ttttgggaca atggctgatt ttgaagattt ggtaaaagct gctaaggagc atcagattga
    10621 gttaatgttg gatatggttt tgaatcactg ttccacagac cacgagtggt tccaaaaagc
    10681 tttagcagga gacccttatt atcaggattt ctttatcttg agagatcagc cgactgattg
    10741 ggtttccaaa tttggtggga atgcttgggc gccttttgga gatacaggca aatactactt
    10801 acacttgttt gatgtgacac aggctgactt gaattggcgg aacccacatg ttcgtgagga
    10861 attggctaaa gtggttaatt tttggcgaga taaaggagtg aagggattcc ggtttgatgt
    10921 gattaatctg attgggaaag atgaagagct ggtggattgt ccggtcaatg atggtaagcc
    10981 agcttatacg gatcgtccta ttactcacac ttatcttcat gatctcaatc aagccagttt
    11041 tggtcaggat gattcgttta tgacagtagg ggaaatgtct gccacgacta ttgataactg
    11101 tcttttatac acggctcccg aacgtgagga gctatccatg gcttttaatt tccaccatct
    11161 aaaagttgat tatgagaacg gtcagaaatg gactattatg gcttttgatt ttgcagcgct
    11221 gcgagactta ttccatgctt ggggtgaagg catgagtcag ggcaatgggt ggaatgcctt
    11281 gttctacaat aaccatgatc aaccacgtgc cctgaatcgt tttgttgatg taacacattt
    11341 ccgaaacgaa ggtgcgacga tgttagcggc ttccatccat ttgtcacgag gaacgcctta
    11401 catttatatg ggtgaggaga ttggcatgct tgatccagat tttgatagta tggatgatta
    11461 tgtggatgtg gaaagtctca atgcttactc aagcttatta gtctcaggta aaagtgcaga
    11521 agaagccttt gccattatca aggctaagtc aagggacaat gccagaacac caatgcaatg
    11581 ggatgctagt gaacatgctg gctttacgac tggtaagcct tggttagagg ttggcaaatc
    11641 ttatcgagac atcaatgtcg aaacagaaaa agagggacgt attttccctt tctaccaacg
    11701 cttgattgct ttgcggaagg aactccctat tattgctgaa ggggactatc gggctgcttt
    11761 taaagatagt caggctgtct acgcctttga acgccattta ggtgaccagt gtttgcttgt
    11821 tctcaatcat ttctatgctg gtgaggtcga actggaatta cctccacgtt atcaacatgg
    11881 acaggcctta atcagcaact atgagaaagt ctctatttgt gaaaaagtga tgctgaaacc
    11941 ttatcagaca cttgctattt tagctgataa ctaactaaaa gcctgaggtt aggcctcagg
    12001 cttttggtga ttgtcacgaa aaaagcagtt ttctcatata tttattttta aactcttatt
    12061 ttttctaaat aaaaacaatt gagataagtt attttagcta aattttggta caatggaaaa
    12121 gattataagt agagtttaag ataaagattc tacaaattta aaatgatata aggagatacg
    12181 aatgacaact tatcaagatg atttttacca ggctgtgaat ggaaagtggg ctgaaacagc
    12241 ggttattcca gacgacaagc ctcgaacagg tggattttca gacttagcag atgaaattga
    12301 ggcattaatg ctagacacta cagatgcttg gttagcaggg gatcatatcc ctgatgatgc
    12361 tattttaaaa aattttgtga aattccatcg tttggtggct gactacgcta aacgtgatga
    12421 agttggagta agtccaattc tccctttaat tgaagaatat cagtccttaa aatctttttc
    12481 agaatttgtt gctaatatag cgaaatatga attggcaggt ttaccaaatg aatttccctt
    12541 tagtgtggca ccagatttta tgaatgctca gctgaatgtg ctttgggcag aagcaccaag
    12601 cattcttttg ccagatacaa catactacga agaaggcaac gaaaaagctg aggaattgcg
    12661 tggtatttgg cgtcagtcgc aagaaaagct tttacctcaa tttggttttt caacagaaga
    12721 aatcaaggat cttttggata aggtaattga gctagataag caattggcca agtatgtctt
    12781 gtctcgtgag gaaggctcag aatatgccaa attataccat ccttatgtgt gggctgattt
    12841 taaaaaacta gctccagagc ttcctttgga tagcattttt gaaaagattt taggccaagt
    12901 acctgacaag gttattgttc ctgaggaacg tttttggaca gaatttgcag ccacttatta
    12961 ctctgaagcc aattgggatt tactcaaggc taatttgatt gtggacgcgg ctaatgctta
    13021 caatgcttac ttgacggatg atattcgtgt ggaatcaggc gcttattcgc gcgctctttc
    13081 aggaacacct caagctatgg acaaacaaaa agcagccttt tatttggcgc aaggaccgtt
    13141 tagccaagcg cttggcttgt ggtatgctgg tcaaaaattc tccccagaag ctaaggctga
    13201 tgtggaaagt aaggtagcac gcatgattga ggtttataag tctcgtctag aaacggctga
    13261 ttggttagca ccagccacac gtgaaaaagc cattactaaa ttaaatgtta tcacgccaca
    13321 tattggttac ccagaaaaat taccagaaac ctatgctaaa aaagtgattg atgagagctt
    13381 gtcattggtt gaaaatgcac agaacttggc taaaatcacg attgctcaca cttggagtaa
    13441 atggaacaag ccagttgacc gtagtgagtg gcatatgcca gcccacttag tcaatgctta
    13501 ttatgatcct cagcaaaatc agattgtctt tcctgcggca atcttacaag agccatttta
    13561 ttctttagat cagagttctt cagccaatta tggaggaatt ggtgcagtca ttgcgcatga
    13621 gatttcacat gcctttgaca ccaatggtgc ttcctttgat gaacatggta gcctaaatga
    13681 ttggtggaca caagaagact atgctgcctt taaagagcgt acagataaga ttgtggcgca
    13741 gtttgacggc ttagagtcac atggtgctaa ggtcaatggt aaattgacag tttcagaaaa
    13801 cgtcgctgat ctaggtgggg tagcttgtgc tttagaagcg gcacaatcag aagaagactt
    13861 ctcagcccgt gacttcttta ttaactttgc gacgatttgg cgtatgaagg cccgtgagga
    13921 atacatgcaa atgctagcta gcattgatgt tcatgccccg ggtgaactgc gaacaaatgt
    13981 caccttaacc aactttgatg ctttccatga gacctttgat attaaagaag gcgatgctat
    14041 gtggagagct ccgaaagacc gtgtgattat ttggtaatta ggtagttgat aaaccttcaa
    14101 atgcctgtga ttataaaaat gactgattct gtaaaacaca ggatcagttt tttgcttcct
    14161 tattggtgtc cgttttgaca caacaaaaag acaaccctca ggaaagaagg ttgccttttt
    14221 tagacttatc agaaataagt gattagttgt taagggcagc agccatagta gctgcaactt
    14281 ctgattcaaa gtcgttagct tttttctcga taccttcacc aacttcaaaa cgtgcaaaag
    14341 caatcgcttt agcgtttact gagtcaaggt aagcttcaac agttttgctg tcgtccatga
    14401 tgtaaacttg tgcaagaaga gtgtaagctt ggtcaacttt agtgttgtca agcatgaagc
    14461 ggtccatttt acctgggatg attttgtccc agattttttc tggtttgcct tcagcagcaa
    14521 gttcagcttt gatgtctgct tcagcagcag taatcacatc gtctgataat tgagcttttg
    14581 aaccgtattt caagaatgga agagctggtt tgtcaaccat tgcacgagat tcgttgtcaa
    14641 gttcgatagc gtggttcaat tgagcaagtt catctttgat gaattgtgca tcaagctcag
    14701 tgtatgaaag aacagttggt ttcattgcag cgatatgcat tgatacttgt ttagcaagag
    14761 catcgtcgcc gccttcgaca actgagataa ccccgatacg gccaccatta tgttggtaag
    14821 caccgaagtg ttgctcatct gttttttcaa tcaaagcaaa acgacggaat gagatttttt
    14881 ctccgatggt agcagttgca ttaacgtaag cttcagcaag agtttcacct gaaggcataa
    14941 caagtgcaag tgcttcatcg ttgttagctg gtttgccttc agcgataact ttagcagttg
    15001 cgtttaccaa ttcaacgaat tgagcatttt tagcaacaaa gtctgtttca gcgttaactt
    15061 caacaacagc agcaacgtta ccatgaacgt aaacgcctgt taaaccttca gcggcaacac
    15121 ggtcagcttt tttagctgct ttagccattc ctttttcacg aagaagttca acggctttgt
    15181 ccatgtcacc atctgtttca acaagtgctt ttttagcgtc cataacgcca gcaccagatt
    15241 tttcacgtaa ttcttttaca agcttagctg taatttctgc catgtgtagt atcctccaaa
    15301 tgatatttgt tttggtttca aaaaacgaga cagagcggtt tgctttctgc cccgtttggt
    15361 tttcgattgt taaagacaaa caaagtttgc cataagagtt aggaactctg tgctaattaa
    15421 gcgttgtcgc cttcgacaac ttcaacgatt tcttcgattg aatctgcttg aatgtcagct
    15481 tcaaaagcaa cgtctgcatc ttcaccttga cggccttcga taatagcgtc agctaattta
    15541 gcggtgatta atttaacggc gcggatagcg tcatcgttag ctggaatgat gatatcgata
    15601 tcatctggat cagcgtttgt atcaaccatc gctacgactg ggataccaag ttttttagct
    15661 tctttaacag caatttgttc tttatgtggg tcaacaacat acatcacgtc tgggatacga
    15721 ggcatatctt cgataccgcc caagaatttt tcaagacgag cgcgttgttt gttaagaagt
    15781 gcaacttctt tcttaggaag aacgtcaaag gttccttctt cttccatacg tttgatttct
    15841 ttcaaacgag cgatacgttt ttggatagtt ccccagttgg taagcgttcc acccaaccaa
    15901 cggtggttga tgaagtattg gcctgcacgc gttgcttcgt cagcaactgc ttcagcagct
    15961 tgtttcttag taccaacgaa caagatgaca gcatcgtttg ctgcagcatc acgaacaaat
    16021 tcgtaagctt ggtcagctaa ttttacagtt tgttgaagat cgataacgtg gataccgtta
    16081 cgttctgtga agatgtattt agccatctta gggttccagc gacgagtttg gtgaccaaag
    16141 tgaacaccag cctcaagaag ttgtttcatt gaaattactg ccatgagtag tttctccttt
    16201 ataaaatgtt ttttcctctt ccagacttcg acttgcaggc taacctagcg gcaacaagcc
    16261 cacaattggt ctagaatgag tatttgccgc ctacacgacc ctactatatt atcaaaaaaa
    16321 gaggttaaaa gcaaggagaa atgctatgga ttcccttatt tttgagatga ctagcttctt
    16381 tgtttattgg taagtgttaa gacaagcttt tgctcatttt gggtgacact tgccaaatcg
    16441 ttttatttaa gatatagtga acttgactaa tttttggagg cgttttcatg acgaagggta
    16501 ttcgatttca attgcttggc agtccccaca tttttcttga tgacaaagaa caattttttg
    16561 cctttgccaa agcaaatgct ctcctttatt acttagtggt taatggatct gttagccgcg
    16621 aggtggcggc gagtctactt tgggaaaata agaacacgca aacagcgaaa aagaatttac
    16681 gaaacgctat ctaccaggtc aataaagtct tgcaagctga tgtgattatt tgtcccaatc
    16741 gcaacctgct ggttttgaaa aaaacacttg atatcaaaac agatattaac ctttttctgg
    16801 ctgatccgct agctcactta gaactctacc aaggggagtt tttgcagggc ttttacctta
    16861 aaagtggtga ggaatttgat ctttgggttt caaaaatgcg tatgcagtat gaacaggtat
    16921 acctaaaagc gtgttaccaa aaaattgaag aaaagctctc tttagatgcc attgaagatg
    16981 tcgaggaaca tttaaagcag cttattgaac gtgatgaatt tgaagagaaa aattatcagc
    17041 tcttgatgag gctttatcag caggggaatt gtcctggtaa ggtcatcgaa acttattatc
    17101 agttggctaa tgtgttggat aaggagctag ggattcagcc aagtttgcag agccaacaga
    17161 tttatcagga agtggtcgct aaagatcgta atgaacgcaa aatcaagcat tttttgcgca
    17221 acagtaatca ttttttgggg cggattgatg agattaagca gctggaaaac tattttgcca
    17281 actgtttggc ctgccaagag gtaggagctc tgctcttaat tggtgataca gggattggga
    17341 aacgaaccct agccagacag gtcttagcca atcaaaccca aacgtttcaa attgtcacag
    17401 ctaaatgttt tcgtgaagag gctatggatt ccttattgcc ttggcgtaat atcttagatg
    17461 gcctgggaga tttggtgatt caaaaccgct tattgaccac caaagcttgg aaggctgctc
    17521 ttaaacgctg ttttcctgtg gcaaccattt ttcaagaaga taacaaccaa ccctttatca
    17581 aggaccacac cagcttgttg gtttccttta ttgttgatat tttacaacat ttggcagaaa
    17641 tcaaggcgct ggtaatcctt attgaggatt gtcactggat ggatgaggat agtttgacct
    17701 tgttgcaacg ggtcatgaat caattggtcc actatccgat tgcttttgtc ttgaccaagc
    17761 atctaggaac gactcctgag ctcggtcttt gtttgaatgc tttgatgagt caggggcgtt
    17821 tagaaagcat ttgtttggag ccttttaatc ggcaagaaag tttggcatat attaacagtc
    17881 aactgggtag tcagccagtg acggaagaag aaatggagca cctctatcaa gccagccaag
    17941 gcaacccttt ctttttgtca gaatacactc agaccctact gcgtcacgaa aaatttgtgc
    18001 ctctaacgcc tgccattaag gctaagttgg gtcttaaact agctaaccta agtagtcgtg
    18061 atgacgcatt gttaaactat ttgtcttgtt gtcggaggcc tatccctcta aataccctcg
    18121 ctcaattgat gttactgcct ttagaagaag tgatcgagat ggtggataat ctggggcact
    18181 actatatttt ggtggaagaa agcgtaggag aggaagtgtt gatttcattt cgtcagcgaa
    18241 ttattcaact ctatagttat gaccgtttat ctttgtcaaa aaggcgttta ttgcacggac
    18301 aaattgccaa acggttagaa gatttactgc ctattttgac accaagccct cacttattag
    18361 atgacattgc ttaccattat caggaatcac ggcaggtgat taaggctttg gaatacaacc
    18421 ttaactatct agatgctacc ttgccatttc agcatgagct gtttcccatt tattcaaaga
    18481 gtattggctc cttggaaaag tcagatcgtg accatcaacg cttaatggaa gagcagtttg
    18541 ataggattcg tcaaagcatt gcggatttgg aactgaccta tgataataac cgtgatttcc
    18601 agcagttgct tattcgtttt tcttaccttg aaggccgtta tgatattcga acagggaggt
    18661 atcaagaagg gattaaaaac attcaaaagg tcatcgcttt ggcaaccgag ctaaaacagc
    18721 cctccttctt gctagaaggc tatcgccagt taattcatta ctgtatccaa gtggaaaaca
    18781 agccagaaat gcgttattac actagtcttt cattggaggc agcggttgct gctaatcatt
    18841 ttgaggccat tgcgattagt ttgcggttaa acggtcttta tcatttgatt attggggagt
    18901 taaatgaggc agagcgtctg cttcaacagt cgattgactt ctttaaggtg acgccaggct
    18961 tgcaagctaa ctatgcgatt cagattgctg ctgccttaga ttatttgggg gagatcgccc
    19021 agattcgcta tcagtttgaa aaggccgttg cttaccaaaa acaagccatt gccttgaccg
    19081 aaaacaagcc agctgagtta tcagtgagta ttttttatat tggattaggg atttcctact
    19141 tttatttagc tgattttgaa caagctgagc aaatcttgag tttggctaag gaagctttgg
    19201 tcaatcatag ttatccttgg aaagaaacgc aacttcaaat ttacctggcc atgattcaat
    19261 ggaaaaaggg caattatcag ccagccttga ccctacttga ttacagagaa accttgatgt
    19321 ctaggtatcg caatccgcgt gataagggct tggttttcta tttgatggct gtggtcaagt
    19381 accaattgat atttcaagga gctaccctaa gtcagcagga aaaagaaatg gcagactacc
    19441 tgttgtcaga atcctttgag tattattatg aaattgctag tactaacctc aacccttacc
    19501 gagattgtca tttggtgagt gagttgaacg atttgcgtca acaattatct gctaaaagct
    19561 aaggaaaacc ctctgattca catcagaggg ttttagttat gttgtgccat aatctgtacg
    19621 aggtaaaaga taaaggtggc tgctaaattg gcggtgtggt tatcaatatc gtgtggtggt
    19681 gaaacctcaa ccacatcaaa gccaactaat tttccgctag ctgcaatgtg ttgcaaaact
    19741 aagacggcaa gatttgggtc aacaccgagg gactggatag cactaacccc aggtgcagca
    19801 ccaactgaga aacaatccat gtcgatagtc aggtagaccc tttcttgccc ttctaagaaa
    19861 cggtcaatgg ctcggcagac cttttgatgg cccatttggt agatgtcttg acctgttaaa
    19921 aattgaatgc ccttggattt tgccacaaag tcaaatagga aaaggttgtt attatgttct
    19981 tgaatgccta aaacgaagta tttaaagagg cgtttgtcag caactgcatc gtcaaacatt
    20041 tgtcgaaagc ctgtccctga attagggcct gtttggtcgt aaggccttaa atcaaagtgg
    20101 gcgtccatat tgatgacagc taggtcatca cttggggata ggctttggcg taggcctaag
    20161 tagtggccgt aggcggtttc atggccacct cctaagacga tgggtttgag attgaggtca
    20221 cacatccgtt tgatggcttt gcttaaactg ttttgcagtt gttccaaaga gcgattgggg
    20281 ccgtcaatgt tacccacatc gtaaaccata acttggtttc ctaaatgcca agggaatttg
    20341 gctagctggg ttcgtatggc agctggactt tcgaccgctc cgacacgacc attattgata
    20401 taaacgccct tatcactttt aaagccaatc aaggcaaaat gcgtcccttc aaatggggtt
    20461 agtgagctgt cgttaaggtc aaggaaagtc atgaccatgc cccatttggc agtatataag
    20521 tcatcgtcga tcccaccatg ataatagcta gtggtggatg ggtagtaatc ttctagcatg
    20581 cgcgtcatct cctttaggat tatgatagca aaataaaact tacagtcaac actttcttgg
    20641 cctttgtcac tggtttttag gaatagcaga gcaataaaat cgtctgcttt tggtcccagt
    20701 tagttagtct aaagaggcca tgacctctgc cacagcccct ctagttaaag aagtacctta
    20761 ttcactaaaa ttggatactc aactcaactg cctgctcaac cgcttcgagg aagcttgggt
    20821 cttcaatgac tgctgatgcc ttgtttagtt catcgtagat ttcgatgtgt ttatcgtgtt
    20881 cgataaagtt gatttccttg cggaagagat cgtaggcaac cttagtccct ttacctagtt
    20941 catgattttc aggtttgaga tcaagagctt ggcaggctgc catgatttca gtagccacga
    21001 tgcggcgaga atttttaagg atttcaaagg ccttgcgagc agcggtagtg cccatgctga
    21061 caaagtcttc ttggttttca caagatggga tagaatccac gctggctgga tgtgccaaaa
    21121 ccttgttttc agaagcaaga gaggcacaag cgtactgggt aatcataaag cctgaattga
    21181 gacctgggta tttgacaaga aaggatggca atttgctgag ctggctattg accaaacgtt
    21241 ccacgcggcg ttcagaaaca tttccaattt cagaaatggc gatgcctaag aagtcaaatg
    21301 gctgtgccat tggttctccg tggaaattac ctcctgaaat aacgtggcca tctttacaga
    21361 taattggatt atctgtgaca gaattgattt caatgtcaac tttggatttg acataggcga
    21421 tactgtcttt gctagcccca tgaatttgag gcatgcagcg caaggtgtaa gggtcttgca
    21481 cacgagattg agtagctacg gttgtgtttt ggctgccttc aatgaggttt cggatattgc
    21541 gggctgttgc caactggccg ctttgtgggc gaatggtgtg aaggttttct tcaaatggac
    21601 tagtaatccc gttatgcact tcaagggata gggcaccagc taggtctgac agtttcaata
    21661 attgaatggc gtcataggtt gcaagggcac cgattgcagt gagaacagtt gttccgttga
    21721 taagggcgag tccttcttta gcggctaaag aaatcttgtc aataccagct ttatcaagag
    21781 cttcttgacc agaaagaagc tctcctttgt agtaagcttt ccctagacca agcataggaa
    21841 gcaccatgtg agcaagcggc gccaaatctc ctgaagcccc tagagaccct ttttcaggaa
    21901 tgtaaggatg aacccttttg ttgagcagtt ccaacagctt ttcaatggtg gatagacgaa
    21961 taccagaata ccctttgacg agagagttga tacggatcag catgatagct ctcacggcat
    22021 cttctggcaa tgggtcacca aatccactag catgggtacg aatcaaattt tcttggagtt
    22081 ggactgtatc ttctggtgag atgcttacgt tacaaaggga tccaaaacca gtggtcaccc
    22141 cgtaaaccac ccgtttttca ctgacaatgt catcaacaat tttacgagag gcattcacag
    22201 cttcaatagc actatcatca atgcgacagg ctacgccttg gcgagcaatc gcaatcacgt
    22261 cttcgatagt aaggctttcg ccatctaaat taatcactct tgtcatagtt tttctcctag
    22321 atttgttcct tagggggcat ttggtccatt tggtggttac gtccgtgcat aaggtagtac
    22381 acactacttg caatgatgac accaactgca ccataaacaa agttcattgg gttgtcaccc
    22441 caaatcataa ctaaactaac gataactgca agaattggga taataggacc aaatggtact
    22501 cggaagatca cattagcatc gggatcgtcc ttccgtaatt tcataacggc tagtgctgtt
    22561 ggaatgtatt ggaagaagcg gaaaacaaca ctgagttttg ctaagttttc aaatgagcca
    22621 gtaagtagga gtacaatggc aattgctcct gaaaccaaga tagcaacgag tggtgcgcca
    22681 ttttgatttt gcttggcaat agcagctggt aagagccctt catcagcaat ggcagcaccg
    22741 taacgtggca ccatgatgga ttctcccatg ttaagacctg taatggaaat taaagcaccg
    22801 attgagacca tccaagcgcc agcagggcct atcattttaa caaaggcatc ttgaacaggt
    22861 gcattggtca tcatgatttg agagccaagc atggcaatgg ttccaccgat aatcagcata
    22921 tataagacag agacgatact gattgatcct aaaagagcac gcggtacatt tttttcaggg
    22981 gcacgcattt ccccagcgac aatagagagg gtttcaaaac cgataaagcc atagaagata
    23041 taaacagcag tattggagat agcccctaag aggttagtcc ctggttccaa ctgaacaaat
    23101 ggggtaaagt taggaagccc gtttttgatg aaaaaaaggg tacaggcaca aaaggcaacg
    23161 attggaatta acttagcaat ggtggctgtg atggtgacaa ttttagaggt tttcaaacca
    23221 gcgatattca tcaaagatag taagatgatt aacccaatac taagtggaat gtgccaacct
    23281 tcaaaggcag ggaaagtaat gataaacatt ctggcaaacc cagctgccat ggctgcccaa
    23341 gcgaagatag taacggtcca accaaggaag ccaacgttaa agccaataaa atcaccaaaa
    23401 gctcgtttgg aatattggaa ggctccgccg tttttcccaa aataaccaga gacttctgca
    23461 aaacaaactg ccaacatgat ggtcaaaata gctgttccaa acataacagc gatggatgca
    23521 gggccaaggc ccttgtaaat ggccctaggt aagagaaaaa taccagagcc gataacggca
    23581 ttgatcccgt aaagagtggc accgctcaag ctaaactttg cctgttctct ttcttgttcg
    23641 ttcatgtgat ttgacgccat aataatgctc tcctttttgt ttgcaaattg cgttcaaata
    23701 taatggataa ggtcgctagg aaatgaggag atagtcggtt taggagggtc cttcttgcac
    23761 ctaacgatag tgatgagcta ttggttagcg atgagaaata ggtagccttt tgtgatgatg
    23821 tgtagtgatt tcattggaga agaacaggca tgagtagtgt gatgcctatc tttatcccac
    23881 taagtcttat accataagcc tgcccaaaca gctactggag tttgggcggg gcttgtttga
    23941 ttggggcaaa cttttagatc tgaaaggctg gcctcctgct cgattaccaa caagtgattg
    24001 gcttcaagct cttgattcgc ctgattggtc acagaataag tcagtttgct tagggcataa
    24061 agcagttgac agtccgcttt gggtgcctca aatggtgtgt cagaccagac gggtaataat
    24121 tgcccttggt attggtcatc atacattaaa ttgatgtctt ggcagattcc acgactgacg
    24181 attctggtat cgccttcaaa cgcatagggt tcaaaaggag ttagggaaat ctctttagca
    24241 tgactcaaat cactcaatgt gatgggcttg tctagggtca ttagaatcct gtgatagcca
    24301 tcaagtaagg taaaggttgt ctgtcgtagg gccacagttg actttgagag gcgataagag
    24361 aattctctct ttttgtaatc tccatcttcg ggataaagga acaattggtt ggtttcaccg
    24421 ccagaccagt cagtttggag gtaatcagag ggcgctttga tagttacctt tgtcatttgt
    24481 tctctccttg tttccaatta gaatagtcct gtgatgttgc cctcctcgtc aatatcaatt
    24541 gtttcgctgg caggtacttt tggaaggcca ggcatggtca tgatagcacc cgtcagagca
    24601 acgataaagc ctgctcctgc ggacactttg aggttgctaa tagtcactgt aaagtcagtt
    24661 ggtgcgccaa gttttttggc atcgtctgag aaggaatatt gggttttggc catgcaaatt
    24721 gggtagttgc caaagccgag gcgttctaag tcagccagtt cacgtttggc tgcgggggtc
    24781 aatctaatcc ccttaccacc gtaaaccttg gtaacgattt tagtcagttt ggtttcaatg
    24841 ctatcatctt cctcataaac aaagcagaat tgattgtctt gctcggctag tgccacgact
    24901 ttttcagcta attcacggcc accagctccg ccatttgccc aaacatcaga aatcacaacg
    24961 tcaacgccac gtttgtcgca ggcatcatag acagcttgta attcagcatc ggtatcaagc
    25021 gggaatttat tgatcgccac gacaactgga agaccataga cgtcttggat attagccaaa
    25081 tgtttgtcaa ggttaggtaa accatccaca acggcctgaa cattttcagt agccaggttt
    25141 gcttttggca cacccccatg catcttaagg gcacgtatag tggctactag gacaacggct
    25201 gctgggcgaa ggcctgacat gcgacatttg atgtcaatga atttttcagc tccaaggtca
    25261 gcgccaaatc cagcttcggt aacggcataa tcgccatatt tcaaggctag tttggtggct
    25321 aggacactat tacagccatg ggcgatatta gcaaatgggc caccgtgaat gagggctggt
    25381 gtgtgttcca gggtttgcac gagattagga tgaatcgcat ccttgagcag agcggctagg
    25441 gcaccaccag ctttcaagtc tttagctgtt acaggttcac cttggtagtt gtaaccgatg
    25501 atgatttttt caaggcgggc tttgaggtca gaaatatttt cggataggca caggattgcc
    25561 atgatttcag aggcgacggt aatgtcataa ccatcttcac gaggaacccc gttgaccttg
    25621 ccttgcaagc cgtcaacaat atgacgaagc tggcggtcat tcatatccac cacacgtttc
    25681 caagtgatac gtctagaatc aatgcctagg ctattgccat ggtgaatgtg gttatcaatc
    25741 aaggcagcca ataagttgtt ggcgacaccg atggcatgaa agtcccctgt aaagtgaagg
    25801 ttgatgtctt ccattgggac aacttgcgcg tggccaccgc cagcagctcc tcccttaacc
    25861 ccaaaaacag gaccaagaga aggttcacga agggcaataa ctgctttttt tccaatggca
    25921 gaaagagcat ctaccaatcc aacggaagta gtggttttgc cttcaccagc tggtgttggt
    25981 gagatagcag tgacaagaat gagtttgcca tcaggtttgt cttttaaggc gaccagttga
    26041 cgggcatcaa tttttgcctt gtacttgcca tagagacaga gcgcttcctc gtcaatccct
    26101 aattgattgg caaccttact gataggctcc atagtgacag agttggctat ttcaatatct
    26161 gataaaacca tagttgctcc tttacttgtt aaacaatatc gagaattttc gtgtagatgt
    26221 catctgccaa gtgacagccc ttgtctaata aggcttgtcc tttttggcga tagtcggtaa
    26281 caaagtcttc atctttgatg ccagacagat taatcaaaac attcaaccaa gcgctttgta
    26341 aacctgcttt taagtttaaa gcagcaactc ctaagtcgct agcagcattt gtattagact
    26401 taccgacagc tgttgcagtg atctcaagtg cttcgaccat gagggtcatc atctcaaaag
    26461 gtgattgtgc agctgttttc aaggcctttt gcatggcagt tcgtctagca gccttgtctt
    26521 cgtcagtctc ttttggcata tcaaaaacgg cagaaaccag gttgaaggct tcggtatctt
    26581 tgtcaatggc tgcaagcaag ctagcttgta gagcggtgct tttagcatgg atctctgtaa
    26641 taatagcttg ataatcagca tactttttct taccaagggt caattcacag accatcttgg
    26701 tcaaggaaat gccattagcg ccagagagag cagcagcaga accaccaccc ggagcagggg
    26761 catctgagcc aaggacttta gcgaaatctg ttaagcttaa atcaactaaa cccatagaga
    26821 acctcctaac ctagcaaatg gttttcaaga acctgtttag ggtagtcaaa gtcctcaact
    26881 tgtaagtagt attcagccac atcgattaag gccttggctg gcgctaagcc gatgacttca
    26941 gaaccaataa cattcacacc gtagcgacgc gcttcaaatt tgatggtttc aaaagtacgg
    27001 taaagagaac atttttcaaa gttaaccatg ttcatggaga cttgggcaat atggcggtct
    27061 tctaacatga caccgatagc cttacaatat ttgtagccac cacctgaacc gcggataatt
    27121 ttagcaattt tatgagcaat gtcgatattg tcagtatcca aattaacatt gaaagcaacc
    27181 aatggcatgc gagcaccaac ggcagttacc ccagcagttg ggtgaatctt acgatctccg
    27241 tagtcaggtg cccaatcctc ttccaatagt ttttctggca tgccttcaaa ctgaccttta
    27301 cggattttag caaggttttg acgttctgga cgtgtggcag agtcttcata aaggaagatc
    27361 ggaatgtcaa gttcacggtt aatgcgttct gcgacttgtt tggaaatttc gacacattct
    27421 tgtgtagtga tgtctttgat tgggacgaac gggcagacat cggttgctcc catacgagga
    27481 tgttcaccat gatgcttggt catgtcaatg ttttcagaag cgtatttcac cagttggaaa
    27541 gctgcttctt gaatagactg gtcatctcca actaaggtaa acacgctccg gttgtggctc
    27601 gcatcagaag agtagtcgag aagggtcact ccaggaatac ttttggccgt cgctaccaaa
    27661 ccatcgataa cagcctgatt ttggccttct gagaagttgg gaatacattc aacaattttt
    27721 gccatgttaa tctcctttta tgtcattgag ttactcatta ggggaatagg ttacctactt
    27781 tagataacgc ttacattttt agctagcagt tattagtgaa ataattgatc taccgctgaa
    27841 gtcacaaggt catcatcagc caagtatggg atagtgatgt gatcagtacc tgcatggaga
    27901 cggttgtatt caatagcggt ttcaatagcg tgctcatttc gggcccagtt acggcgagct
    27961 acaccgccca tagtatccca agcaatagca gatttaataa tctcatcaat gcgttcgctg
    28021 ccatctaaaa caagtccgaa accaccattg atggctttac caataccagt tccgccaccg
    28081 ttgtgaagag ccactaaact cataccacgc gcagcgttac cagcgtaaca ttgcaccgcc
    28141 atatcacagg tcacattgga accgtcttta atgtttgagg tttcacgaaa tggtgagtcg
    28201 gtaccagaca cgtcatggtg gtcacgacca atcatgacag gtccaatctt gcctttgcgg
    28261 accaattcat taaatttaag ggcgacagtc acacgaccga tacagtcttg gtaaaggatc
    28321 cgggcttggg taccaactac gagttgattt ttctcagcat cacgaatcca gttgtagtta
    28381 tcacggtctt gatagcggcg gtcaggatcg atagcttcca tggcagcttt gtcagtagcc
    28441 accaagtctt cgtgtttacc actcaagcag acccaacgga aaggtccata gccatagtca
    28501 aaaagcatag gtcccatgat atcttccaca taggatggcc agataaatcc gtctttgtcg
    28561 ttacgaccat tcttagaaat ttctgtaata ccagagtcgt aaacagactt cataaaggca
    28621 ttgccgtagt caaagaaata ggtgccattt tcagttaacg tcttaatagc ttcaaaatgc
    28681 cgtgccaagg tatcatcaac catttggtgg aaggtatctt tatcttctgc caagagccgg
    28741 gttctttcgt caaagctaat acctactggg cagtagccac cgtcataaac gttgtgacaa
    28801 gaggtttggt cagacaaaag atcaacatga atctgcttgt cattgacgta ctctaaaaga
    28861 tcaacgatat taccatggta agcaatcgaa gtggactctt tagcatctat ggctttttgg
    28921 gctaattgca gagcttcttc agggctctca gcaatttggg taacccagcc ttgcgaatga
    28981 cgggtttcaa tccgagattg gtcaacttca gcaataatgg caactgcttt agcaatttca
    29041 gcagccttac cttgtgcccc actcatgcct cctaaaccag atgagataaa gagtttaccg
    29101 gtcaaatcac catcgtcagc caccccaagt ttgagacgac cagcatttaa gagggtatta
    29161 aatgttccat gaacgattcc ttgtgggcca atatacatcc aaccacctgc ggtcatttga
    29221 ccatagttgg tgacacccat ctcttcagcg atttcccagt ctttcatgtt gtcgtattca
    29281 cccaccaaaa ggccattagt aataatcaca cgaggcgctt caggtttgga ttggaaaaga
    29341 ccaactggat gaccagactc aaccaccaag gtttgttcat cagtcatgac ttctaggtat
    29401 tttttaatca aacaatattg catccagttg gcacaaacag aaccagtttc tccataagtg
    29461 actagttcgt aagggtacaa ggcaatctca aaactcaagt tattgtcaat catgacctgc
    29521 atggccttgg cagctgtaca atttcccttg tactcgttaa tgggtttgcc gtaaatgcgg
    29581 tcttttggac gccatctgta accatagatg cgtccccttg ttttgagttc ttccaaaaat
    29641 tctggaatga cttcttcgtg gaacttcgta ggtacatagc gtagggcatt tttcaaggcg
    29701 atttcggtct gagcctgggt caatcgaaaa cctctgtctg gtgctcttcg gataccttct
    29761 ttgaagacag tcttttccgg taaaacatcg tctaacttaa cagtcatagc ggcagctatg
    29821 tcagtttcac tgtaaaatga catagaaaac tcctcctcaa ctccttttta ttgtcgagat
    29881 tagtctaact tagactagtc tataaaaagt caaccacgct ttgaaatgcg aaaaaagtct
    29941 aaaaaaaaat caaaacctca ttttttgact aaaatttgac gcttaatcag tacacttgag
    30001 gcatgacaga taaagtcagt cagagtttaa ttgttgttac caaagttgca gagatcgcaa
    30061 ctattgagtg ggtgaggggg gactagtaat ggctattaat cagttgacaa acgatgttaa
    30121 aaaaggaggt ttttatggtt gctgatgtct tattaacaca ttttaatcaa ctattttgtc
    30181 taaatgatcc aggtcatcct ttaactggtc aagaaatgaa aaaagccact atcgttgaag
    30241 atggctatat agccatcaaa gacggcctca ttgtggctct tggttctggt gaaccggatg
    30301 ctgagcttgt cggtccacag actattatgc gttcttataa aggtaaaatt gcaacgcctg
    30361 gtattattga ctgtcatacc catttggtct atggtggcag tcgggaacat gaatttgcca
    30421 agaaattagc aggggtgtct tacttggata ttttagctca aggtggtggt attttaagca
    30481 cggtgagggc cactcggtca gccagctttg acaaccttta tcaaaaatcc aagagattgc
    30541 tagactacat gttgcttcat ggagtgacta ctgtcgaagc gaaaagtggc tacggccttg
    30601 attgggaaac agaaaaacgc caactggatg ttgttgctgc gttagaaaaa gaccatccta
    30661 ttgatttggt gtctacgttt atggcagccc atgcgattcc tgaagaatac aaagggaatc
    30721 ctaaggctta tctcgatgtg attatcaagg atatgcttcc tgttgttaaa gaggaaaatc
    30781 tggcagaatt ttgtgatatt ttctgtgaaa aaaatgtttt tacagctgat gagtcccgct
    30841 atctgttaag caaagccaaa gaaatgggct tcaagcttcg tattcatgca gatgaaattg
    30901 cgtctatcgg cggagttgat gtcgcagcag agctcagcgc agttagtgca gagcatttga
    30961 tgatgataac agatgatggt atcgctaagt tgattggtgc tggtgttatt ggtaatttgc
    31021 ttccagccac cacctttagt ttaatggaag acacttatgc ccctgctcgc aaaatgatcg
    31081 atgccggcat ggctatcact ctctcaacag atagtaatcc aggcagttgc ccgactgcta
    31141 acatgcagtt tgtgatgcaa ttaggttgtt ttatgttacg tttaacaccg attgaagtct
    31201 taaatgctgt taccattaat gcggcctact ctgtcaatcg tcaggagagg gttggtagtt
    31261 tgacagttgg taaagaagca gatattgcca tctttgatgc cccaaatatt gattacccat
    31321 tttatttctt tgccaccaac ttgattcacc aagtctataa aaaaggacaa ctcacggttg
    31381 atcgaggtcg tattctttaa aaaatttcaa aaagaagctt tgagcgttag ctcaaaactt
    31441 cttttaaata tgctatttta ttgacgaatc aagtagtcaa aggcaccgat ggcagcagta
    31501 gcaccagacc ccatggaaat aatgatttgt ttataagctg aatcagtaca gtctccagca
    31561 gcaaagatac caggaatatt ggttgaccca tgtttatcca caatgatttc accgcggtcg
    31621 gttaggttga caccactatc tttgagccaa gctgtgtttg gaacaagacc aatttgaaca
    31681 aagacccctt caaggtcaag gtgtttgtct tcaccgctat cacgctcagt gtagctaaga
    31741 cctgtaacat ggtcctctcc aacaatatcc ttagtagcaa catttttgat gatggtcata
    31801 ttgttagtct tagctgcgcg gtcttgaagc actttgtcag ctttaagttc aggcaagaat
    31861 tctaaaacgt aaacgtgttt agcaagacca gccaagtcaa gggccgcttc taatccagag
    31921 ttcccaccac cgataacagc aacatctttg ccttcaaaga gaggaccgtc acagtgaggg
    31981 cagtaagtca cccctttatt acggaattca tcttcaccag gaacattaat gttacgccat
    32041 ttggccccaa gagctaagat agcagttttg gcttgtagaa ccgcaccatt agctagagta
    32101 acctcaatgt tttctttttt ctcaatagaa gtagctagtt gtgctttgat aatatcaaca
    32161 tcataagact tagtatgggc ctccacttcg gccattaatt ttggtccttc agtgtaaagg
    32221 gtaccaatca tgttttcgat gccgaccgtt tccatgacct gaccaccaaa ggtttcagca
    32281 agtaaacctg tttttagtcc tttacgagct gcatagatag cagcgctatt gcccgcaggg
    32341 ccaccaccaa taacaaggac atcatagagg cctttatcag caaaggcttc ctcagaaagt
    32401 gggccagcga tttgttctaa gagttgctca atggtagcgc gaccagaagt gaattcttcc
    32461 ccgtctaaaa agacagtagg gacagacata atgccttttg ctttcacttc atcttggaac
    32521 atgccgcctt ctaccatagt gtgtgaaatc ttgtcgttca agacggacat aatgtttaaa
    32581 gcttgaacaa catctgggca gttatggcag gttaagctga cataagtttc aaaatgcaac
    32641 ggacggtcaa tggccttgat gcgatcaata acatcttgat ccacttttgg agcacgtcca
    32701 gatacttgca agagagcaag aataaaggac gtcaattcgt ggcctaaagg aagaccagca
    32761 aagacaaccc cactaccgtg accttttttc gcaaccttaa agcttggttg tcggtctaaa
    32821 gtgatgtttt caatagaaat acgctcagac atagcagcta tttcttccac aaagtctttg
    32881 actttttgag actgctcatt gtcaccaagg gaaacttgca aaacaagatc agcttctaac
    32941 agggtgaggt attgggcaag ttgttcttta atatcaggac ttaatgccat agctttcctc
    33001 ctaaaattta ttaaatttta ccaactaaat caagacttgg tgtcaaagtt tcagcgcctt
    33061 ctttccattt agctggacaa acttcacctg gatgtttacg gacgtattgg gcagcgtgaa
    33121 ttttatcaat caaggtgcta gcgtcacgtc caataccatc agcattaatt tccatcattt
    33181 ggataatacc atctgggtca acgataaatg ttccacgttg agcaagtccg tcttcgccaa
    33241 gcacttcaaa ggcttgtgaa ataaggtgtg aagggtcacc aatcataggg tatgtgatag
    33301 tgccaaccac atctgagtca tcatgccaag ctttatgaac aaaatgagta tcagtagaga
    33361 cagaataaac ttctacacca agagatttca gtgtttcgta ttgctcttga aggtcaccga
    33421 gttcagttgg gcaaacaaat gaaaagtctg ctgggtagaa acaaaaaact gcccattttc
    33481 ctttaacgtc ttcatttgta acagtgatga attttccatc gtgataagct tgagctgaaa
    33541 attcagcaat ttcttttcca attagagaca tatagttgtc ctcctttttt atagttttat
    33601 acttgtatta taaaagaaca acaagattat gtctactcag aaacccttga ttttactggg
    33661 aaatcagcga ttgttttctg acaattcagt tgtgaaataa atcacattaa ttaaaatgat
    33721 tctaaataag ctgaagacgc cgttaaatca aaggttaatt taattataat ctacagaaaa
    33781 tgtaattgtt attaaagcgt ttacaaaaaa acttttttga ttccgttaag gcaacttaat
    33841 attatggtta cctaatcgct aatattgtaa cctattttgg catgagatca gggaatggct
    33901 gttatattaa ttggatatgt cttgtgcaaa tagtgcttga cggatttgtg gaattagggt
    33961 aaaataatct ttgttgtggc ggtatagcca agtggtaagg cacggctctg caaaagcttg
    34021 atcgtcggtt caaatccgtc taccgccttt tatatacaaa acaagccttg attaatttcg
    34081 tgaaaacgtt gttaaatcaa ggtttttatt ttttgcgaca ggataaaaaa agagaaaaaa
    34141 agaaataaag tcggtcaaaa gtcggtcaaa tttcccgaag atgtctttta tagcctgatt
    34201 attaggtatg aaaaatacta tggccaaaat tggtaaacga ttgcaaataa aggtcagagt
    34261 tcttaccccc ttaaacgata tgcgttaaaa gttagttgaa ctgccgtgca cctaacgata
    34321 gacacggtga tgtgagagcg agtagagttg aatctctact tgtttatctt accatagtca
    34381 gctttataaa gtaaccaccc attacaatgc aactatattc ttaatgtttt taagcatgtt
    34441 attgacaagt gttataaaat gtgttactct tagtgagtaa attagttttt gagttagtat
    34501 ttgtgtttca acatctttat ttctctctat ttttacaaat gatttttaga aaggaagtgg
    34561 atgtatatgg cacaaggtac agtaaaatgg tttaacgctg aaaaaggttt tggttttatt
    34621 tcaactgaaa acggtcaaga tgtctttgca catttttcag ccatccaaac taatggtttt
    34681 aaaacgttag aagaaggaca aaaagtggca tttgacgtcg aagaaggtca acgtggacct
    34741 caagcagtca atatcactaa attggcctaa tctggttaaa gtaactaaag agaagcagat
    34801 gttggaaaca acaactgctt ttttgatttc taccagttac ctttaatgat taagagttgc
    34861 gtttaaggtc ttgtatttgt tataataata gtcagtttta gtcaaaatgt attgacgtgg
    34921 tgctactaag tttttagagt agcccgcaag caaaggagtg acagcatgcc aaccaaaaat
    34981 acttcagaca gcattgaaga gtatattaaa gaattattag ctaagtcagg aatagcagaa
    35041 attaagcggt ccatgctagc agattcgttt caagttgtcc caagtcagat caactatgtc
    35101 attaaaaccc gttttacgga gagtcgaggc tatgaagtag agagtaaacg aggtggtgga
    35161 ggctatattc gtattgccaa ggttcatttt tcagacaaac accatctgat tggcaattta
    35221 atggcaacta ttgaagactg tatcagtgag caagttttca cggactcgat acaattgctt
    35281 tttgacgaac acttattgac agagcgtgag ggaaatatca ttttggctgt ggcgtctgat
    35341 gatgttttag gaactgatgg ttcaactatt cgtgcacgaa tgctctatcg cttattgcaa
    35401 cgaattgaca gaaagggaag caattaatga ttatgtattc aacgaagatg caagacattt
    35461 ttagacaggc gcagttccaa gctgctcgct ttgatagcca ttgcctggaa acttggcatg
    35521 ttttgttagc tatggtagct gtagataatt ctttagcaaa tatgatttta agtgaatatg
    35581 atgcccaagt cgccatagaa gaatatgaag ctgcagctat tttagccatg ggcaaaaccc
    35641 ctaaggaaca gttgtctcgt gtagacttca gacctcaatc taaaaccttg actaacttgt
    35701 tagcttttgc gcaggctatt agccaaatca ctagggatca agaagtcggc tctgagcatg
    35761 tcttatttgc tattttattg aatccagata tcatggcgag tcgtttgtta gaaatagcag
    35821 gctatcagat aaaagataac ggcaatgggc agccgcgatt agctgacttg cgaaaagcaa
    35881 tagaacgtca tgcaggttac agtaaggaaa tgattaaggc tattcacgaa ctacgtaagc
    35941 ctaaaaaaac gaaaacacaa gggacctttt cagatatgat gaagccacca agtacagctg
    36001 gtgaattgag tgatttcaca agagatttga ctgaaatggc aagacaaggt ttgttagaat
    36061 cggtgattgg acgtgaccaa gaagtatctc gtatgattca ggtactaagt cgtaaaacga
    36121 aaaacaatcc tgtcttggta ggtgatgcag gtgttggtaa aactgcgctt gcttatggcc
    36181 ttgctcaacg gattgcaaat ggcgctattc cttatgaact taaggagatg cgtgtcctag
    36241 aattagacat gatgagtgtg gtagcaggaa cccgttttcg tggggatttt gaagagcgca
    36301 tgaatcaaat cattgatgat attgaagctg atggtcagat tattcttttt gttgatgaac
    36361 tacatactat tatgggttct ggcagtggta ttgacagtac acttgatgcg gctaacattt
    36421 taaaaccagc attatcgcgc ggtactcttc atatggttgg agcaacaact caagaagaat
    36481 atcaaaaaca tattgaaaaa gatgcagctc tttcgcgtcg ttttgctaaa atattaattg
    36541 aagaacctaa tacagaagat gcttatcaga ttttgatggg cctaaaatta tcttatgaga
    36601 cctaccataa tgtctcgata tcaaatgagg cagttaaaac agctgtaaaa atggcacacc
    36661 gttatttaac cagtaaaaat ctccctgatt cagctatcga tttattagat gaagctagtg
    36721 ctgctgtgca aaacatggtg aaaaaatcag ctcctgagac tttaacacca atagaccaag
    36781 ctcttatcaa tggtgatatg aaaaaagtat ctcgcctctt agctaaagaa gcaaaaggtc
    36841 agatgagaaa accaacacca gtgacagaag atgatatttt ggcaaccttg agtaagttat
    36901 cgggaattcc acttgaaaaa ctgacgcaag ctgatagtaa aaaatacctc aatttagaaa
    36961 aagaactgca taagcgtgtg attggtcagg atgctgctgt tacggctatt tcaagagcca
    37021 ttcgtcgcaa tcagtcaggt attcgaacag gaaaacgtcc tattggatca tttatgtttc
    37081 ttggcccaac aggagtaggt aagacagaac tagcaaaggc ccttgcagaa gttctctttg
    37141 atgatgaagc agcgcttatt cgttttgata tgtctgagta catggaaaaa ttcgcagcgt
    37201 ctaggcttaa tggagcacct cctggttatg tcggctatga tgaaggaggt gaactgacac
    37261 agaaagttag aaataaacct tattcagtct tgctttttga tgaagtggaa aaagcacatc
    37321 ctgatatttt taacgttctc cttcaagtat tagatgatgg tatattgact gatagtcgtg
    37381 ggcgtaaggt cgatttttca aatactatta ttatcatgac cagcaatctt ggcgcaacag
    37441 ccctgcgtga tgataaaacg gtcggttttg gggtcaaagg cattcaccaa gaccatcaag
    37501 ctatggagaa acgtatttta gaagaattaa gaaaaactta ccgcccagaa tttatcaatc
    37561 gtattgatga aaaagtggtc tttcatagtc tgacccaaga taacatgcgc gatgtggtta
    37621 aaatcatggt acagcctctg attactacat tggcagaaaa aggtattacc cttaaaattc
    37681 agcctttggc cttgaaacat ttgtccgagg tcggctatga tgagcatatg ggggcaagac
    37741 cattacgtcg aacgctgcaa actgagatag aagataagct atcagagctt atcctttctc
    37801 gagaattgac aagtgggcat acgctaaaaa ttggattatc atatggcaaa ttaacgtttc
    37861 acatagctta atttccatat aaaaggtgga tcatccttga tgtcaccaaa aaaagaggtt
    37921 attcttacaa aaataacttc ttttttcttg actatttttg accaagtgat aaaatagaat
    37981 ataaagttag cactcgatga acatgagtgc taactttact aaagattctt ttattggagg
    38041 aaaatttcat gttaaaacca ttaggtgacc gtgtggtcgt aagatttgat gatgaaaaag
    38101 agcagacagt cggtggtttt gtccttgcag gcactcataa agagtcaaca cgaaaagcga
    38161 cagtcctcgc tgtcagtgaa acgggtgttc gcactattac aggtgatagc gtgctacctt
    38221 cagtttcagt gggtcaagag gttttagttg aaaatgggca tgatttagaa gtaaccgtag
    38281 atgatgagaa agtttctatc atccgtgaat ctgacatcat tgctattgtt acaaaataaa
    38341 acgacaaatc tctaaataga aagaaggatt gaatatggca aaagatatta aattttcagc
    38401 agatgcgcgt gctgccatgg tgcgcggagt tgatatgtta gcagataccg tcaaagtaac
    38461 gcttggtcct aaagggcgta atgttgttct tgaaaaagct tttggttctc ccttaattac
    38521 taatgacggg gtaaccattg ctaaagagat cgaattagaa gatcattttg aaaacatggg
    38581 agcaaaattg gtgtctgaag tggcttctaa aaccaatgat attgctggtg atgggacgac
    38641 tactgcaaca gttttgacac aagccattgt tcatgaagga ctaaaaaatg tgacagcagg
    38701 tgctaatcca attggtatcc gtcgaggcat tgaaacagca acagcaacag ctgttgaagc
    38761 cttgaaagcc attgctcaac ctgtatctgg caaggaagct attgctcagg tcgctgcagt
    38821 atcatcacgc tctgaaaaag ttggagagta tatctcagaa gctatggagc gtgtgggcaa
    38881 cgatggtgtg attaccatcg aagaatctcg aggtatggaa acagaacttg aagtggtcga
    38941 aggcatgcaa tttgaccgtg gttacctgtc tcaatacatg gtcacagaca atgaaaaaat
    39001 ggttgcagac cttgaaaacc catttatctt aatcacggat aaaaaagtgt caaacatcca
    39061 agacattttg ccactacttg aggaagttct taaaaccaac cgtccattac tcattattgc
    39121 agatgatgtg gatggtgaag cccttccaac ccttgtcttg aacaagattc gtggtacttt
    39181 caatgtggtt gctgtcaaag cgccaggatt tggtgatcgt cgtaaagcta tgcttgaaga
    39241 cattgctatc ttgacaggtg gtacagtgat tacagaggat ctaggacttg aattaaaaga
    39301 tgctacaatg acagcccttg gacaggctgc taagattaca gttgataaag atagcacagt
    39361 aattgttgaa ggttcaggaa gttcagaagc tattgctaac cgtattgcac tgattaaatc
    39421 gcaattagaa acaacaactt ctgactttga ccgtgaaaaa ctacaagaac gtttggcgaa
    39481 attagctggt ggtgtagctg ttatcaaagt aggagctcca acagagacag ctttaaaaga
    39541 aatgaaactt cgcattgagg atgctctaaa tgccacacgt gcagccgttg aagaaggtat
    39601 cgttgctggt ggtggaacag cacttattac ggtgatcgaa aaagtagctg ctcttgagct
    39661 tgagggcgat gacgctactg gacgtaacat tgtgcttcgt gctctagaag agcctgtacg
    39721 tcaaattgct ttaaatgctg ggtacgaagg ctccgtagtt attgacaagt tgaaaaacag
    39781 ccctgcagga acaggattta atgctgcaac aggtgagtgg gttgatatga ttaaaacagg
    39841 aatcattgac cctgtcaaag taacacgatc agcgcttcaa aatgcagctt ctgtagctag
    39901 tcttattttg acaacagaag cagttgttgc taataagcct gaaccagctg cgccagcgcc
    39961 agcaatgcca gcaggtatgg atccaggaat gatgggcggc ttctaagctc tctatagcat
    40021 taaggattat gaaaaagaag ttgagactaa aggtctgctt cttactatga aaaaagaaca
    40081 aagattggat ttcgactcat gatatcccat ctttgttttt ttagttgtga tataaaatgc
    40141 caatctttgt ggttacagac ttttaatgta ttggaaaata tattgttttt ctttgtcatg
    40201 tgctatcatg gagccaatga aaagttattc ccatatatgc gtggtggagg tctgtgatga
    40261 atcattttgg agagattttt aaaaccttta gagaatcaaa gggattacga ttaaaagatg
    40321 tcgcaaaggc tggtatatca acctctcagc tgtcgcgttt cgaaaaaggg gagacggatt
    40381 taaccatatc gacatttatg ctgattttgg atgaaagtaa catgtccatt gatgagttta
    40441 tgtatgctgt ccatgatttt catcgtgacg acttaaatga actcttatca aaaagtgagg
    40501 gatttcgtaa caattcaaga tagtgatggc ttgaagcagt tacttcatgc tcaactagca
    40561 tcaactgata agaaggaaat gttcacggat atcttttggc atatctagca tgtctaagtc
    40621 tgctatggca tgccattttg ggagtccctc acgcgactct gtcagtagct ctccttcaaa
    40681 ggattcacat agaaagtcgt aatagacata gcactctttt tttgtcaggc ttgataaagc
    40741 tggaaatacc ctttaattga aggtttaaag ctgtcagacc tatttcttct tttagttctc
    40801 ataaagccgc ttcaaagaaa gattcacagg gctcgacttt accttctggc tggacccatc
    40861 ctggtaagtt atcgtgttgt ctatggagtc ataaaatcat atcgttatgt ttgacacaca
    40921 tattgcccca gtctctaacc gttgctatca ccagattcac caccttttag tataagaaaa
    40981 aaggggtgaa agaggtttct tgttgttttt ttggcttaag atttctttaa gtcttatcta
    41041 gtatactaat gtcattcctt aataggataa gtatttggtt gtagaagtat cgtcttaaac
    41101 taacgctatc tcaaccgatt cccaacttat tcacccctcc tataccgata aggtctcaca
    41161 aagtgtgaga ccttattttt tgacttcagc tctagttatg gagaaacttg ttgatacaag
    41221 cttttttggg ttatgggtta tggtattatg atctattcaa gcctttcttg ttttggggtt
    41281 tttcttcaac caacttggct tcaatagctt ttaagcgttt gaaggttttt tcagcgcgat
    41341 ctagtgaaat cttatcagct ttttcttgag cagctttggg gtcactgacg actaagtcac
    41401 tgatttcttt ggtgcttttg tcttgttcgg ctatccaggt tttttccaag cctttcatct
    41461 catcaatcac tttagttcca aatggttttg gatgagcagc cacgagatca ttaatgtgtg
    41521 acaccgtcca ataccatgac ttatcgttat attgggtgct tttttcttga taggcttcat
    41581 aggttctact gatattgcca aggtatggca agtaaggggc attacgagga ctgccaatag
    41641 atagccacat gacaccacca cccaattcag caggaatgtc ttttttcagt tggaaaatat
    41701 gagcttccat gacattagga tttgagatgg ggtaagcata acctttaaca gcttttttgg
    41761 attttggttt acccttaccg tctaatgcca tctgatcaag tggtttaaga tccagcccct
    41821 caaaacgatt acgttgcagt ttcatcgcat cttctagact aaaggtctta tcagtgcttt
    41881 ggagcaactc ataattgctg tctttataag ttacttttga atcagggtca agtgatttaa
    41941 tccctgagaa agagcgagag cggttggcat cgtttagtgg tggattgtaa gatttggcaa
    42001 tatgaaattt accatcaact tcggtataag atttggcttt tttagtgact ttttccacat
    42061 cttctgaggc aatcgtattt tccttatcgt tgaaatcgac atgtccgaga tagaaggtat
    42121 ttggaaaaac agcgtattta tcatctggga atttgatggc aacgtattgg tgaccagaga
    42181 gaatttccat gtaccagatg ccatctttgt cagccaaggt aacaatattt ccttcagcag
    42241 ctcctttttc agtgactatt ttagcaatca atgcgacacc ttcacgggct gttttgacac
    42301 ttggtaaaat gacactggcc atagatgatt ccgctaagcc atttttgacg taaggatcaa
    42361 ttttttggat ggcatcatta gctgaagctg atactgttgc agacatagag acgccaaact
    42421 cattaaaacc agcttcgtca taaacgcctt tattaggtgt tacatcagga atcgcactat
    42481 agcgataaga atgctcaggc aatgggtact caaaaccatt agaggggtct ttccattttt
    42541 cgccagcagg attgtccttt gctagacgta caatgaagtt tttattatga tgtggctcaa
    42601 gatcttctgt tcgaccataa agaagagaac cgtctttggt taaatctttg ccaataataa
    42661 aaccagtaca agcataacta acactttgaa gcgaaaaggc tgtcagaata cttaaaactc
    42721 caagtgatat ttttttgttt atcatgcgaa tcctcctagt aaacgataac gctttcacag
    42781 acattataaa atgctctttt taaaaattca agttttttat gaagtgtttt gaaattgtta
    42841 gctatctaaa tattatgaga attctctctt gaattttggc ttaacggccc ctaaaaacgt
    42901 ataggcagtg aactatctag gtttcttcga aaatccatta gccatttatg atataatagt
    42961 aaagaaaaac atagaaagtt ggggtctgtt gatgaccaaa ccatttcatc ataagaaact
    43021 aaaacaaata actataatag ctgcaactag ccttttttta ttcctgatcg gtggtgcctt
    43081 ttactactct aaaaatcatt gtattaatgc ctatcttaaa gctcgatcag cccaatcagg
    43141 accagttttt gaaaatatca aagcatatct agtctgggat gatactaatg agcagatcac
    43201 aaatgacgag gcgatgtata ctaagtttag aaggtatagt caaaaagaat tgaggcaaaa
    43261 aaagcaggat ttaaaagccg ctagtcaaga tagtgccgtt caagtaaagt ctgttggtcg
    43321 tcgtttttgg atttttcctg attatcggat tgccataaaa cccatggatt taacgataaa
    43381 aacgaatgtg cctcaagcag acgttctttt aaatcaaaaa aaagttgctg tttctgattc
    43441 agaacagttc tcagtaaagc ttgatcggct accaacggca gaatataccg caagtatcag
    43501 aggcaaacac aacgggcgaa acattaaagt caacaaatca tatgatggtg ataatcccgt
    43561 gctagatttg agtgtgtctt ttagaacttt tttagtaaca agcaatgcta agcaaggaga
    43621 tctttacttc gatgataacc atattggcac attaaaagat ggtcaattac aagtagaaga
    43681 ttaccctgtt acagaaaacg cacaagctta tatgaaaaca acattcccag atggtgagtt
    43741 aagatcacaa aaatatgctc tagctgatgt tgaagaagga gcaaccctgg agattttagt
    43801 tacagatctt ttagaagagg acaaagcagg ggagctatta gtatcagctt ttgatcagct
    43861 gatgcactac ctcagtacag gtcaagactc atctaactta cgtagcgtct ttgaggcagg
    43921 gtctagcaac gcattttata ggggattgaa agagtccata aaagcgaaat ttcagacaga
    43981 tacaagaaaa gccagccgtc tcaatattcc atctatcctt ttgacaacaa tgactcaagt
    44041 gggtaaaaca acttacgtgc ttgatttcac agctacctac gaatttttgt atgacaactc
    44101 aacagatcct gagcagcata cctctggaca tattaatcaa gacttgactg gaaaagtgac
    44161 tgtaaaaaaa gttggacagc attaccttat cagccagtcc ggctctaaaa atattactgt
    44221 tgttaaagag gacaatcaac tcaaagcgcc atctgtcttt cctgagtcta ttttgggaac
    44281 gtggacaggc caagccaatg gtttaagcat ccatatgtct ctagcatcag atggaacaat
    44341 tacgactaaa gttgaagatc gaaaaggcaa ccgttctaaa gaaactcgga cagctaaaat
    44401 tagtaaagtt gaagacaaag gcaatggttt ttatctttat acgccagatc ctggaagtga
    44461 cataagcgcc ttagctccag aaggaggatt ggggggtgca aatgtcaaat atgcttatgg
    44521 tttcaaaata tctggtaaaa cagcctctcc agtggtgtgg caggcagcat taacacatga
    44581 atttgattat accaagccac tttcgggagt aactttgcaa aagcaaccat aaaggatgca
    44641 aagccacaaa gaacagtaga aacaatgtta tcactcaaat acagtgctat gaaaacagat
    44701 aaactcaaaa ggctaggaga ttatttccta gccttttgag cgtttaagac tgcgtcaaat
    44761 gtagctgatt tagcttgcac gcaaatgtct tcttgggtta gagggtgttt taaacgtaat
    44821 tgataagcat ggagcatcag tcgagcacaa tctttttttc cattagaata caaaggatca
    44881 ccaaataaaa catgaccttg atgggctaaa tggactcgaa tttgatgagt ccgtccagtt
    44941 tgtaattgac aattgagtaa gcttgccgtt ttgtggaagt ttttgactag agttacctca
    45001 gtaatagctt tcttgccatt tatcggatcg acaacacgtt ttctgcggtc atgtcgatgg
    45061 cgaccaattg gatgatggta agttacccta ggactgtcta aggatccgtg aactagtgcc
    45121 aaatattctc gatggatgtc tcttttttct aaaagacgat taaggattgg taagataaaa
    45181 ggtgttttag caaataaaat ggccccactt gtttccttat ccagacgatg aaccacatag
    45241 caggtctgtc ccgtgtaagc agacacgtgg ttgagcagcg ccaactctgt tggatcattg
    45301 ccgtgtgttt tcatcccttc aggtttgtta acaataatga tgtgctcatc ttcataaagg
    45361 caggttactt tttcggcctg ccccatcacg ataatctttt caggataatc ttcgtgatcg
    45421 aaaaaaagtt tgacctgatc accgtattta acacaacttt gccagttaac aggatgacca
    45481 ttaataagaa catgtttttt agttctcaga aaatgcctaa ttttacgggg aattaacaat
    45541 tgttcctcta ataatgcttt gaccgtcaat tcttcaaagg gatttttcag tgtgacagta
    45601 aacccctttg atggtttatt tgttgtcttc attgagccag ttatggtatt tttcaacaag
    45661 gttgatagcc atttgggtgg caagagcagc agagaaagct tcagtacctt cacgctcgtc
    45721 attagctacc tccaagcgag tctcatcgta aatggctttt agactttctg cggataaggt
    45781 ttctttaaaa gcttggaatt tattcattgg ttaaacccgt ccttctatgt ttaatgtgag
    45841 ctcctgatta atgcaaggaa ttcaatgcta ttatagcatg tttctaatca aaacgtgcca
    45901 ctcaaaaagg taaaaacgtt attttgctaa tccatctaat aagaaagtgt cttaaataag
    45961 acctcttgat agagtttgta acagaaatga tatgaaacta ggtgctaacg cagaaataga
    46021 aaacaactaa cgtgttgcga agttggaaca gtcataaaaa aatagcaaga taaaacttat
    46081 caacacacag ttacctaaga cgatgagtca aacaggtatg ttctttcaaa acaaccccat
    46141 gaggttgaac gacgtatcgg aaaaatacgg attagtttgg ccttatgata ggtccttctc
    46201 tatcgtaaaa gaagtcaacc aaaggttttt aatataaaat attaagaaaa attgttataa
    46261 ttatgataaa aacataatcg taaaatagag gaaaaacaat gaaaacaatt agacgttatg
    46321 atgttaacga agatagaggg catacaggcc ttgtagaagc aggagatttc tattatttaa
    46381 actattgtgt cggcaatgtt ggacaagaca ttgaaagtca gattaatgga gcctttgacg
    46441 agatggagcg ccgattagca ttggtggggc tcacattaga tgcagttgtt caaatggatt
    46501 gtctatttcg agacgtttgg aacatacctg tgatggaaaa aatgattaaa gagcgcttta
    46561 atggcagata tcctgctcga aaatcgattc aaacggaatt tgcacatcac ggtggacctc
    46621 aaggactact ctttcaagtg gatggtgtgg cttattcaaa acatatttcg atgacctaat
    46681 caaggttact ttaagatcat agtggtataa taacgttatg agatttctag aacttttaca
    46741 aaagaaattt tttcctaaag catatcagga aaaacaattc ttaatgcatc aaaaaacgcg
    46801 tttaacgcca caacacaatc aaaagcagta ttcgccaaat gccaatcatt tggactcatc
    46861 agctaccaaa aactcagaac aagaccctgc aatagctctg caacgcagta gagcctatga
    46921 aggaagccct aaaagtcggc ccgcttggtt gcaaaagttg gaagctgttt tgccgtctcc
    46981 tcaacatcca attcggcgtt tttggcgccg ctatcacatc ggaaaactgc taatgattct
    47041 gattggaact cttgtcttac tcttaggatc atacttgttt tacttatcaa aaacagctaa
    47101 agtatctgat ttacaagatg ccttgaaggc tacaactgtt atttatgatc acaaaggaga
    47161 gtatgcaggc agtttatctg gtcaaaaagg gagttatgtt gagctcaacg ctatttcaga
    47221 tgatcttgag aatgctgtta ttgccactga ggataggact ttttacagta atagcggtat
    47281 taatcttaaa cgcttcttat tggcggtagt tacggcgggc cgctttggag gtggctcaac
    47341 gattacacag caactggcta aaaatgctta tctctcacaa gatcagacaa ttaaacgaaa
    47401 ggcccgagag ttttttttgg cgttagagtt gaccaaaaaa tacagtaaaa aagatattct
    47461 tactatgtac cttaacaact cctactttgg aaatggagtt tggggagttg aagatgccag
    47521 tcaaaaatat tttggaacca cagctgctaa cttaacactg gatgaagctg ccacattagc
    47581 aggtatgctc aaaggacctg aaatatataa cccttaccat tctctaaaaa atgctactca
    47641 ccgtagagat actgttttag gagcgatggt tgatgccaaa aagattaccc aaacaaaagc
    47701 tcaacaagct agagcagtag ggctaaaaaa tcgcttagct gatacttatg ttggtaagac
    47761 agatgactac aaatacccat cctactttga tgctgttatt agtgaagcaa tagcaactta
    47821 tggtctttca gaaaaagaca ttgttaataa tggatacaaa gtttacaccg agctagatca
    47881 aaattaccaa actggcatgc agacgacttt taacaacgat gaactatttc ctgtttcagc
    47941 ttatgacggt agctctgctc aagcagctag tgttgcttta gatcctaaaa caggaggtgt
    48001 tagaggtctg attggtcgtg tgaatagtag tgaaaatccg actttcagaa gttttaacta
    48061 tgcgactcaa gcaaaacgta gtcccgcatc aacaatcaaa ccactcgtgg tttacgcgcc
    48121 agccgttgct tcaggatggt caattgaaaa agaactacca aataccgttc aagattttga
    48181 tggctatcag ccacataatt atggaaatta tgaatcagaa gatgttccta tgtatcaagc
    48241 attagcaaac tcttataata ttccagcagt ttctacattg aacgatatcg gaatcgataa
    48301 agcctttacc tatggtaaaa catttgggtt agatatgagc tctgccaaaa aagagttggg
    48361 ggtagcttta ggtggcagcg tgacaaccaa tccattggag atggctcagg catatgctgc
    48421 ctttgccaat aatggagtaa tccatcctgc gcacttgatt aaccggattg aaaatgccag
    48481 gggtgaagtg cttaaaacct ttactgataa ggctaaacgt gttgtcagcc agtctgttgc
    48541 agataagatg acagccatga tgctaggtac cttttcaaat ggaacagcag tcaatgctaa
    48601 cgtatatggc tatacactag ctggtaaaac aggaacgaca gaaaccaact tcaatcccga
    48661 cttagcaggc gatcagtggg ttattggtta tacgccagat gttgttatta gtcaatgggt
    48721 aggatttaat cagaccgatg aaaatcatta tctaacggat tcaagtgcag gcacggcttc
    48781 agctattttt agcactcagg catcttacat tttgccttat accaagggca gccaatttca
    48841 tgtagataat gcctacgctc aaaatggtat ttcagctgtt tatggagtca atgaaacagg
    48901 taatcaatca ggagttgaca ctcaatctat tattgatggt ttaagaaaat cagcacaaga
    48961 agcttcgcaa tcactatcaa aagcagtcga tcagtcaggg ttacgtgata aagcccaatc
    49021 tatttggaaa gagattgttg actattttag atagcttgca gtattttaga gatcgtgtta
    49081 aaataatagt tacggaggcg ttatggcaca gaaaaaagcg agtctagcgt gtgtcgaatg
    49141 tggcagtcgt aactactcta tcggagtgag tagcacgcct aaaccaacac gtctagaagt
    49201 aaataaattt tgcaaatact gcaaaacata taccttacac aaagaaacac gttaaaaagg
    49261 agagcctgat gggattcatt agcgggactt ttaaagtctt aaaagacaca acatggccaa
    49321 atcgtaaaca acgttggaaa gattttatct cagttcttga gtacacagct ttctttacgg
    49381 tgattatcta catctttgac cagctcttag caaagagtgt attagcactg attaatttat
    49441 tttaggtcaa gattggtaga aatgataagc ttgcttggaa aagcctaggc tgggagtatt
    49501 cccagccttt tttgtagtat caataatcta attagtaagc ttttttaaag cccatactgg
    49561 tttgagctat tcaaaagcat aaaggtaaag gcaatgtagc aacgatctcc aaaccaagtt
    49621 cactaaatta gcttgtaaca gagtcaacgc ataaacagcc tggcctcact tgcttagcac
    49681 attaaaaata gcaatgaata ttatggttga ggaaaacagc ctgatagtct gctaaatctt
    49741 cgggatgata agccttgacc tcagccatct catactccct acctagaagt ttgtacttgt
    49801 tttctccaaa ttgatggaaa ggtaacagtt gcacttggtc aatctcaagc tgattaaaga
    49861 gctctgagaa ggctttagca tcgtctagtg aatcattaaa ttggggaata acagggatgc
    49921 gcaagacgat ttcttttcca gcttgaaagg cataatgaat atttttgata atcaagtcat
    49981 tacgaacccc agtcactttt tggtgtctga gctgattata atgtttcaaa tcggtataga
    50041 taaagtctac gtagtcaata agggtgacaa attgctcgtg tttggcaaag gcggtggttt
    50101 caattgcggt gtgaagacct gctgctttgg ctgcttttaa cagggctaag gcaaagtcaa
    50161 attgggcaaa gatttcaccc cctgacaggg tcatgccccc acctgactct tcgtagaaat
    50221 caaggtcttt aaggacttct tcgatcacct cgtccacggt tttttcctct ccaactattt
    50281 tagtattaag cccatcactt gttaacatct gctcaggtgc tttttgttgg gattcgggat
    50341 tggcacacca aggacatctc aagggacaac cttttaaaaa aacggttgtc cgaataccag
    50401 ggccgtcatg aatactaaaa tgttggatgt tgaaaacaat accgcgatca gtcataagtg
    50461 tgaattcctt tcgttgtaaa accatcatac cagataaacg taaataaatc aattacgaat
    50521 gaaagtaaat atctcttttc ttttaagaaa ataactggta caatgaaagg ggggtgaatg
    50581 atgaatcgat tagaacgtat tattcaactg gtttcccaga agaaaaaaat agatgttaac
    50641 agtttatcag agcaattaga agtttcgaaa gtaaccattc gaaaagacct tgataaactt
    50701 gaaagtaagg gcttgttgcg acgggaacac ggctatgcgg tgctaaacag tggcgatgac
    50761 ctcaatgtac gcttgtccta caattacaac ataaagcgac ggattgcaga aaaagcagct
    50821 gaattggtgc aagacaacga taccattatg attgaatctg gctcaacctg tgcccttttg
    50881 gcagaagtgc tttgccagac caaacgaaat attaaaatca ttaccaattc gtgcttcatt
    50941 gccaactaca ttcgtcaata tagtagctgt caaatcattt tactgggcgg ttattaccag
    51001 cccaattcag aggtgactgt tggtccttta ttgaaagaaa tgatatccct ttttcatgtc
    51061 aatcgtgtct ttgttggaac agacggtttc aacaaagatc ttggctttat gggcaaggac
    51121 atgatgcgct cagaaggggt gcgttacatg gcagatgctg ctgaggaagt ggtgattttg
    51181 accgattcca gcaagttttc aaaaaccagt ctggtacacc agttgtcatt agctgatgtc
    51241 aatcgcgtca ttacagacca agcactagac aagcaaacac aagagttatt aagttctggt
    51301 ggtcttgttc ttgattttgt ttcttagaaa ataggtggct agatgaaaga agaacgcaga
    51361 cggctccttg ccaaggtggc ttacttgcat tatgttcaag gcaagagcca aaccttgatt
    51421 tcaaaggaaa tgaatatcta ccgaacaacg gtttgtcgga tgttagcaaa agccaaggaa
    51481 gaaggcattg ttcggattga aattgccgac tatgatgctg atttatttgc tttagaagaa
    51541 tacgtgcgaa agcagtacgg cttggaaaaa ctagaccttg tgcccaacca ggttgaagat
    51601 accccaatgg ataccttgac aaatgttgct aaaacagcag ctgaggtttt tagacatgtt
    51661 gttaaagatg gcgataagat cggtctgtcc tggggagcga ctctttcttg cttgatggat
    51721 gagttaaatc caaaagcgat gaaagatgtg tttatttatc ctttggcagg aggacctagc
    51781 catattaatg ccaaatacca tgtcaatacc ctggtttacc gcttggctcg catctttcat
    51841 ggtaatagcg cttttatgaa cgctatggtc attcaagaag acaaacactt ggctaaggga
    51901 atcttacaat ctaaatactt taacgatatt ttaacaagct gggaccagtt agatcttgcc
    51961 ttagtaggaa ttggtgggga gcctaatagc cttgagcaga gtcaatggcg tgatttatta
    52021 accagctcag atcatgacca actcaaatat gaaaaagccg ttggtgaagt ttgttgtcgt
    52081 ttctttgatc aggcaggtca gccagtttac actggcctac aggatcgaac tattggtatt
    52141 tcgctagaac agttgaggcg tgtccctaaa acaatggctg ttgcaacagg taaacacaag
    52201 gctaaggcaa tattggcagc cttaaaagca ggttttatca attatctagt gacggataaa
    52261 gagaccatgc tagctgtttt agcgttagat gaagatattg atcttaataa cgttcttctt
    52321 taatagaaat tcaaagatac tatgatattt atagaggccc aagaccaaac ggtcagggct
    52381 tttttgtttg atgtgttgat aaaacagaga ttgctcaaat gtgcatttaa tgtattggca
    52441 caaaagtgca ttaaacaaaa tatagcacaa aatatattga ccctaaaaac aaaaatgata
    52501 caataaactt accaacgaaa tgtaaacgat tacaaacata ataacatcac cttcgaaaaa
    52561 aaagactcat tgtcgataca tcacatgaaa aagaaaaaaa ggagacacca atgcaagtta
    52621 ttgtaccaga ccaaatcatt atgggcttaa ttttaaatgc aggcgatgcc aaacaacata
    52681 tctaccaagc tctgaaatgt gctaaagagg acgattatgc cactagtgaa aaagaaatgg
    52741 ccttagcaga tgatgcctta ttagaagccc acaatttaca gacccagttt cttgcccaag
    52801 aagctagtgg taataagtca gagattacag ctctttttgt tcactcacag gatcacttga
    52861 tgacgaccat tacagaaatt aatctcatca aagagattat tgaccttcgt aaagaattgg
    52921 caactaaata agttttcatg actaaaaagg agattgacat gattaaaatt ggattgtttt
    52981 gtgcagcagg attttcaacc ggcatgttgg tgaacaacat gaaagtagct gctgaaaaaa
    53041 aaggcattga ctgtcagatt gaagcctatg ctcaagggaa attagcggac tatgcaccat
    53101 tacttgatgt ggcactttta ggcccacagg ttgcctatac tctggataaa tcagaagcta
    53161 tttgtaaaga caatgatatc cctattgcag ttatcccaat ggctgattat ggaatgttag
    53221 acggcaacaa ggtgcttgat ttagccctta gccttgttaa agaataggag tcaatcatgg
    53281 caaaaatgaa tatgcaaaac atcatcaagc caatcatgac atttgtgaat atgcgcggca
    53341 tcattgcctt aaaagacggt atgttagcca ttttaccttt gaccgttgtg gggagtcttt
    53401 tcctgattgc tgggcagatt cctttccaag gggttaatga tgccattgct agtgtgtttg
    53461 gtgctgactg gacagaaccc tttatgcagg tttaccatgg aacatttgct attatggagt
    53521 tgatttcctg ttttgcaatt ggctattctt atgctaaaaa ctcagatgtt gaacctctcc
    53581 cttcaggggt tctatccttg tcagccttct ttattttatt gagatcatct tatgttccag
    53641 cagaaggtga agctattgga gatgctatca gtaaggtttg gtttggtggt caagggatta
    53701 ttggtgctat tgtgattggt ttaacggtcg gagctgttta cacaacattt atccgtcgtc
    53761 atatcgtcat taaaatgcca gatcaagtgc cacaagccat cgccaaacag tttgaagcca
    53821 tgattccagc ctttgtgatc tttaccttgt caatgcttgt gtatattatt gccaagtcag
    53881 taacaggtgg tggcaccttt attgaaatga tttatgatgt cattcaggta ccgctgcaag
    53941 ggttaacggg ttctctttat ggcgcactag gtattgcctt ctttatttcc tttctttggt
    54001 ggtttggcgt gcatgggcaa tctgtggtca atggaattgt cactgctctt ctcttatcaa
    54061 atctagatgc caataaggcc ttgatggcag caggtgagtt atccctagac aagggtgccc
    54121 atattgtaac ccaacaattt ttagactctt tcttgattct atcaggttct ggcattacct
    54181 ttggtttagt ggtagctatg atctttgcgg ctaaatccaa acaatacaag gctttaggta
    54241 aggttgcagc cttcccagcc ctctttaatg ttaatgaacc agttgtcttt ggttttccaa
    54301 ttgtgatgaa tccagtcatg ttcttgccat ttatcttggt tcctgtcttg gcagctctca
    54361 cagtttatgg cgccattgct attggtttca tgcagccctt tgcaggagtg acccttccgt
    54421 ggtcaacacc agccattatc tcagggttca tggttggtgg ctggcaagga gctattgtgc
    54481 aaattcttat actcatcatg tcaacgttgg tgtacttccc attctttaaa atccaagata
    54541 atatggctta tcaaaatgaa caagctagtg aagagtcatg atttagcgtc cacacaaatt
    54601 cttgagacca tctccagtta gaggtggttt tttagaaaaa gaagaacgat ttatcaggcc
    54661 tatattgcac aatagtgcaa aataaagtga ctatctcttc ttatggttaa ggatacgcaa
    54721 aagtcgcaat atatgataaa atagttacga aagaaacatt tataatttcg taagaaagaa
    54781 ggagaccccg catgattgaa acaaaaagcc cttattttgg acatttaaca gacagaatgg
    54841 ctcactatcg tgaagctgtg ttagacaaga aaccttatat tgatgctgag cgtgctattt
    54901 tagccactga agcctatcaa aaacaccaaa ataagccagc aaacctcaaa cgcgcttaca
    54961 tgcttcaaac tattttggaa aacatgacca tctatattga ggatgaaagc ctcattgctg
    55021 gtaaccaagc ctcttcgaac aaggatgcgc cgattttccc agaatacact ctggaatttg
    55081 tcctcaacga acttgatctt tttgaaaaac gagatgggga tgtcttctac attaccgaag
    55141 aaaccaagca acagctccga gacattgccc cattctggga aaacaataac ttacgtgctc
    55201 gctgcggggt cttgctacca gaagaagtgc aagtgtacat ggaaacaggt ttctttggca
    55261 tggaaggcaa gatgaactct ggggatgccc atttagcggt taattaccaa aaactcttgg
    55321 agcacggctt aaagggattt gaagaacggg cacgagctgc caaagctgcg cttgatttga
    55381 ccattcctga aaacattgac aaataccatt tttacgactc tgtttttatt gtcattgatg
    55441 cagtcaaaac ttatgccaaa cgctatgcca agttggctag agaactagca aaaacggcta
    55501 aaccagaacg tcaagcagaa ttgttagaca tcgcgcgcat ttgtgataaa gtgccttacg
    55561 aaccagcgaa aacctttgca gaggctgtgc aatctgtctg gttcattcag tgtattttgc
    55621 aaattgaatc aaatgggcat tccctatctt atggccgctt tgaccagtac atgtatcctt
    55681 atgtcaaagc tgatttggag gctggccgtg aaacagaaga cacaattgtt gagcgtttga
    55741 ccaacctctg gattaagacc ttgaccatta ataaagtacg gagccaagcc cacaccttct
    55801 catcagcagg gagtccactt taccaaaacg tgacgatcgg cggtcaaaca cgcgataaaa
    55861 aagatgcggt caacccactg tcttaccttg ttctaaggag tgtggctcaa accaaattgc
    55921 cacaacctaa cttgactgtt cgttaccata aaggcctaga caataccttt atgaacgagt
    55981 gtatcgaggt catgaaactt ggttttggta tgccagccat gaacaacgat gaaatcatca
    56041 ttccatcttt catcaaaaaa ggtgtctcag aagaagacgc ctatgattac tcagccattg
    56101 gctgtgtgga aacagcagtt cctgggaaat ggggctaccg ttgcacgggg atgagttaca
    56161 tcaacttccc taaaattttg ctgattacca tgaatgacgg gattgaccca gcatcaggca
    56221 agcgctttgc aaaaggacac ggtcacttca aagacatgac atcttatgaa gaattaaaag
    56281 cagcatggga tgccaccctg cgcgaaatta cccgcatgag cgtcattgtt gaaaatgcca
    56341 tcgacctagg tcttgaacgt gaagtaccag atattctttg ctcagccttg acagatgatt
    56401 gtatcggtcg tggcaagacc ttaaaagaag gcggagctgt ttacgactat atctcaggcc
    56461 ttcaagtcgg tattgccaac ctatcagact ccctagcagc gctgaaaaaa ttagtatttg
    56521 aagaaggtcg attaactcca gaagaactct ggcacgccct tgaaagtgac tttgctggag
    56581 agcgcggaga agagatccga caaatgctca tcaatgacgc accaaaatat ggtaatgatg
    56641 atgactacgc agatagttta gttgtcgaag cttacgacac ctacattgat gaaattgcca
    56701 aatacccaaa tacccggtat ggccgtggcc caatcggagg tatccgctat tctgggacat
    56761 catcaatctc agccaatgtc ggacaaggaa aaggaacctt ggcgacacca gacggtcgcc
    56821 atgcaggcac accactagca gaaggctgtt caccagaaca cagcatggac aaaaaaggcc
    56881 cgacttcagt attgaaatct gttgctaaat taccaacaga tgagattgtc ggaggagtgc
    56941 tactcaacca aaaagttaac ccacaaaccc tggccaaaga agaagacaaa ctgaaattaa
    57001 tggccttact tcgtaccttc tttaatcgcc tacacggtta tcatatccag tacaatgtcg
    57061 tgtcacgtga aaccttgatt gatgcccaaa aacatcctga aaagcaccgt gacctcattg
    57121 tccgtgttgc cggctactca gctttcttca atgtgctctc aaaagcaacc caagatgaca
    57181 ttattgaacg tacagaacac acactttagg agaaaataag tatggaatac atgttagaca
    57241 ctctagattt agaagctatt aaaaaatggc atcacatttt gcctctcgca ggtgtgacct
    57301 caaacccttc tattgccaaa aaagaaggtg acattgattt ctttgaacgg attcgagaag
    57361 ttcgtgccat tatcggtgac aaggcttcta ttcatgttca agtgatcgcc caagattacg
    57421 aaggcatctt aaaagatgct gctgaaatcc gcaggcaatg tggagatagt gtgtatgtca
    57481 aagtacctgt aaccacagaa ggccttgcag ccattaaaac cttgaaagcc gaaggctatc
    57541 acatcacagc aaccgctatt tatacgacct tccaaggact attagccatt gaagctggtg
    57601 ctgattattt ggctccttac tataaccgta tggaaaatct caatattgat ccagaagctg
    57661 tcataggaca attagcagaa gctattaacc gcgagaatgc tgatagtaag attttggcag
    57721 caagctttaa aaatgtggct caggtcaata aatcctttgc tctgggagca caagccatca
    57781 ctgctggccc agatgtgttt gaagcaggat ttgccatgcc atctattcaa aaagcggttg
    57841 acgattttgg caaggattgg gaagccattc atcaccgcaa gagcatttaa gaaatagcat
    57901 cagtagtaat cacatcatta ggaatcacag ataaaggagt tcaacgatga aagtatttgc
    57961 aagtccatct cgttacattc aaggtaaaaa tgccttgttt accaatgcta aaaccctaaa
    58021 acaactagga aatagcccca ttttactgtg tgatgacgtg gtgtatggca tcgtcggaga
    58081 aagatttgaa agctatttga tagataatgg catgactcct gttcacgtag cctttaatgg
    58141 cgaagcctca gataatgaaa tcagccgcgt ggttgccatt gccaaggaaa atggcaatga
    58201 tgttattatt ggacttggtg gtggaaaaac cattgacagc gctaaggcta tcgctgactt
    58261 acttgctgtt ccggtgatta ttgccccaac cattgcttca acagatgccc caacctcagc
    58321 cttatcggtt atttataccg atgaaggagc ctttgaaaaa tacattttct attcaaaaaa
    58381 tccagatctt gttttggttg acacacaagt aatttgtcag gcaccaaaac gcttattggc
    58441 gtcagggatt gcagatgggt tagcaacatg ggtagaggcg cgtgctgtta tgcaaaaaaa
    58501 tggagacacc atggcaggtg gcaatcaaac cttggcagga gttgccattg ccaaagcctg
    58561 tgagcagacc ttgtttgcag atggcctaaa agcgatggct agctgtgaca gacaagtcgt
    58621 gaccccggcc ttggaaaatg tcattgaagc caatacactt cttagtgggc ttggttttga
    58681 aagtgcgggt ctggctgctg cacatgccat tcacaatggc tttacagcgc tgacaggtgc
    58741 tattcatcac cttacccatg gtgaaaaagt cgcctatggc acgttgaccc aacttttctt
    58801 ggaaaatcgt tcgcgtgaag aaattgaccg ctacatcgac ttttaccaag ctattggcat
    58861 gccaacaacc ctcaaagaaa tgcatttaga cacggctact caagaggatt tcttaaaaat
    58921 tggccgtcaa gcaaccatgg caggcgaaac catccatcaa atgccatttg ttatcagtcc
    58981 agaagatgta gcagcggctt tggttgctgt tgatgcctat gttacgagtc gataggctaa
    59041 ccaaaaagag atttcttatt tgccagataa cctcaaaaaa acaagtctag ggagtcattt
    59101 cctagacttg ttttacttat tgttggttgg taatagcact tttagcaggg tcattgtggg
    59161 caatacggct ggcagctgca gccgcaatgg cgctaacaat atcatctaaa aaggtatggc
    59221 aggagttgcc atctttgtgg ttgagtttgt caacaatccc cggttttgtc ttgtccagat
    59281 acccataatt ggtaaaaccg atagacccat agaggttaac aatggataag gccaggattt
    59341 catcaatccc atacagccct tggtcggttt tcaagatact aaggagtggc tcagacagct
    59401 ggttggcttc agccaattta tccaattcta ccccagtgat aatagcattt tgcacttctc
    59461 gttttgcaag caccgcttca acgctctcta aacattctgc catggtcaaa ttgggaatat
    59521 aatcattctg caaaaaaagc accagctctg caatagcttc taaagaaacc cctcgttcag
    59581 ccagtaattg ataagaaact tctcgtaagt gatggtcagt tgacatgtga gacctccttt
    59641 ttttctttat tgtaacataa aagattttaa gaaacctcta caacagctaa agaggggata
    59701 gttctttttt aagagatgtt gactactgag accccctact tggtaaaagt caaagaaacg
    59761 atagtaaagt aaccatctta cctaggccta aatacaatca ccacccctag gtgacacaca
    59821 gcacctattt caaaaatgat attagtatgg caaaacaaaa agacaacgcc ttcttttttc
    59881 tccttattat ctcgtttaat tttcatattt tttaaaaaaa ctattgataa actagttaag
    59941 taagcgtata ctatggttag tgagcgaaat tagaaaagag gacaagcata tgaatctact
    60001 tggatcaaga cgggtttttt ctaaaaaatg tcggctagtg aaattttcaa tggtagctct
    60061 tgtatcagcc acaatggccg taacaacagt cacacttgaa aatactgcac tggcacgaca
    60121 aacacaggtc tcaaatgatg ttgttctaaa tgatggcgca agcaagtacc taaacgaagc
    60181 attagcttgg acattcaatg acagtcctaa ctattacaaa actttaggta ctagtcagat
    60241 tactccagca ctctttccta aagcaggaga tattctctat agcaaattag atgagttagg
    60301 aaggacgcgt actgctagag gtacattgac ttatgccaat gttgaaggta gctacggtgt
    60361 tagacaatct ttcggtaaaa atcaaaaccc cgcaggatgg actggaaacc ctaatcatgt
    60421 caaatataaa attgaatggt taaatggtct atcttatgtc ggagatttct ggaatagaag
    60481 tcatctcatt gcagatagtc tcggtggaga tgcactcaga gtcaatgccg ttacaggaac
    60541 acgtacccaa aatgtaggag gtcgtgacca aaaaggcggc atgcgctata ccgaacaaag
    60601 agctcaagaa tggttagaag caaatcgtga tggctatctt tattatgaag ccgctccaat
    60661 ctacaacgca gacgagttga ttccaagagc tgtcgtggta tcaatgcaat cttctgataa
    60721 taccatcaac gagaaagtat tagtttacaa cacagctaat ggctacacca ttaactacca
    60781 taacggtaca cctactcaaa aataatacca aaaggctaga cctctgctca ctaggcctag
    60841 ctttttacat caaaaaaagc aatgactata gaaagtaaaa atactagaaa aagcaatgat
    60901 tgccgtcatt gctttttatg atttgtcaaa aagtaaaaag caaattgaaa aaatcgccct
    60961 ggagctgttg agataaacta caccatttat taagatacca aatcaaaaag ctaacaccat
    61021 aagagcgtta gctttttaaa gagcctcagg acagtttatg tttaatggct tctaggtagg
    61081 tttgaaacat atgatggatc gttttgcaat tgagtaattg gaagatctct aagatctgca
    61141 cacacttttg ctcagccccc tctttaccat ttatgaggcc aataatgcca tcaaaaaaag
    61201 acacacaaca ccgttcatat aagcaatcat catttagctg ataatcttca atttttgcca
    61261 aaatctcaga agctttatca tactcttgtc gctgaataaa caaaatcaac atattgacaa
    61321 acatccgaat cgattcattc ccataatacc ttagggtatt gtatttatcg aggttcaaaa
    61381 taacttttga aaacaccatc tcaataaaac aagactcaaa aataaacata caattattaa
    61441 aaagcacagt ctcatagtga ctccaagttt caatattaat aagatagttt gacaaataag
    61501 tccgctctgt cagacagtct tcctcagtaa gagttgctaa taacaccttg accaaggcaa
    61561 aaaggttctt ttcctttgtt gacttactat ctttataaga atcaaggata tgatttaaac
    61621 cttctatgtt tcggcattca aaataatgtt ttgccgtatc catatcaata a
//
LOCUS       DATUYR010000004        54677 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.004, whole genome shotgun sequence.
ACCESSION   DATUYR010000004 DATUYR010000000
VERSION     DATUYR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54677
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.004"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            135..572
                     /locus_tag="VCI33_000195"
     CDS             135..572
                     /locus_tag="VCI33_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2831151.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGPQEKVTAIDFTTEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGTTDDELGGI"
     gene            572..1771
                     /locus_tag="VCI33_000196"
     CDS             572..1771
                     /locus_tag="VCI33_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEP2831152.1"
                     /translation="MTRSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYGGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPESREAALKAADKLIQRYQDKGGFIQ
                     AWGVLGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPIKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFMNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKTYE
                     AAMHSMLRALIKDYANKNLNPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            1807..2295
                     /locus_tag="VCI33_000197"
     CDS             1807..2295
                     /locus_tag="VCI33_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP2831153.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHHVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            2314..3096
                     /locus_tag="VCI33_000198"
     CDS             2314..3096
                     /locus_tag="VCI33_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP2831154.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGMILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVVIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWAFAFMGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            3083..3904
                     /locus_tag="VCI33_000199"
     CDS             3083..3904
                     /locus_tag="VCI33_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP2831155.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAIVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVILTVIIGVIGSWLGILA"
     gene            3990..5897
                     /locus_tag="VCI33_000200"
     CDS             3990..5897
                     /locus_tag="VCI33_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEP2831156.1"
                     /translation="MSLAFTRFKETVNPNFCRNYLLDYQADSYADRKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LTAKGFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFDALTKQEERLIL
                     ASLEKQLHFLHANYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSICLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQINSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGMFLIIDTIQAQGNHCLVSQFILDNHLEVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKFIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            5961..6956
                     /locus_tag="VCI33_000201"
     CDS             5961..6956
                     /locus_tag="VCI33_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2831157.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(7040..9721)
                     /locus_tag="VCI33_000202"
     CDS             complement(7040..9721)
                     /locus_tag="VCI33_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEP2831158.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTY
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(9951..10337)
                     /locus_tag="VCI33_000203"
     CDS             complement(9951..10337)
                     /locus_tag="VCI33_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEP2831159.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            10419..11720
                     /locus_tag="VCI33_000204"
     CDS             10419..11720
                     /locus_tag="VCI33_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP2831160.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEVLFHTDHEAFTQEIITNPETEINAILVRHALDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSRGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            12176..12985
                     /gene="yidA"
                     /locus_tag="VCI33_000205"
     CDS             12176..12985
                     /gene="yidA"
                     /locus_tag="VCI33_000205"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEP2831161.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHMVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            12985..14211
                     /locus_tag="VCI33_000206"
     CDS             12985..14211
                     /locus_tag="VCI33_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEP2831162.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWRKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGIEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLDIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            14198..15433
                     /locus_tag="VCI33_000207"
     CDS             14198..15433
                     /locus_tag="VCI33_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP2831163.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQNDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(15532..16290)
                     /locus_tag="VCI33_000208"
     CDS             complement(15532..16290)
                     /locus_tag="VCI33_000208"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEP2831164.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(16531..17727)
                     /gene="tuf"
                     /locus_tag="VCI33_000209"
     CDS             complement(16531..17727)
                     /gene="tuf"
                     /locus_tag="VCI33_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEP2831165.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(18081..19385)
                     /locus_tag="VCI33_000210"
     CDS             complement(18081..19385)
                     /locus_tag="VCI33_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEP2831166.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            complement(19525..22323)
                     /gene="ppc"
                     /locus_tag="VCI33_000211"
     CDS             complement(19525..22323)
                     /gene="ppc"
                     /locus_tag="VCI33_000211"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEP2831167.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQAIIAEEVALLKEMLENITRR
                     MIGDDAFAVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINYQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMSSSVGLSTSAWSSPASLENPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQESCVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            22482..24281
                     /gene="pepF"
                     /locus_tag="VCI33_000212"
     CDS             22482..24281
                     /gene="pepF"
                     /locus_tag="VCI33_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP2831168.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVSYENFYQNHENAEIRDKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            24274..24753
                     /locus_tag="VCI33_000213"
     CDS             24274..24753
                     /locus_tag="VCI33_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEP2831169.1"
                     /translation="MPNLYDFTVKAQNGNDLSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(24800..25183)
                     /locus_tag="VCI33_000214"
     CDS             complement(24800..25183)
                     /locus_tag="VCI33_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEP2831170.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQTQTPSVPKADHHVRLVVK
                     EDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNGK
                     MADKGADQITVKDGDSIEFYQEVFK"
     gene            complement(25167..25670)
                     /locus_tag="VCI33_000215"
     CDS             complement(25167..25670)
                     /locus_tag="VCI33_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2831171.1"
                     /translation="MSAKFMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFALYPLSQQFPKYQLGYQ
                     AFLAALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            25995..26843
                     /locus_tag="VCI33_000216"
     CDS             25995..26843
                     /locus_tag="VCI33_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEP2831172.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     regulatory      26971..27067
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27112..27675
                     /gene="thiT"
                     /locus_tag="VCI33_000217"
     CDS             27112..27675
                     /gene="thiT"
                     /locus_tag="VCI33_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEP2831173.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     gene            27765..28244
                     /locus_tag="VCI33_000218"
     CDS             27765..28244
                     /locus_tag="VCI33_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEP2831174.1"
                     /translation="MAKKTKFKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            28585..29208
                     /locus_tag="VCI33_000219"
     CDS             28585..29208
                     /locus_tag="VCI33_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP2831175.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKSDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNTSLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(29316..30218)
                     /locus_tag="VCI33_000220"
     CDS             complement(29316..30218)
                     /locus_tag="VCI33_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP2831176.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            30392..31885
                     /gene="lysS"
                     /locus_tag="VCI33_000221"
     CDS             30392..31885
                     /gene="lysS"
                     /locus_tag="VCI33_000221"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEP2831177.1"
                     /translation="MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            32021..32161
                     /locus_tag="VCI33_000222"
     CDS             32021..32161
                     /locus_tag="VCI33_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831178.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(32258..32470)
                     /locus_tag="VCI33_000223"
     CDS             complement(32258..32470)
                     /locus_tag="VCI33_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEP2831179.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(32688..33974)
                     /locus_tag="VCI33_000224"
     CDS             complement(32688..33974)
                     /locus_tag="VCI33_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP2831180.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIIICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            complement(34076..35002)
                     /locus_tag="VCI33_000225"
     CDS             complement(34076..35002)
                     /locus_tag="VCI33_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP2831181.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            35201..35485
                     /locus_tag="VCI33_000226"
     CDS             35201..35485
                     /locus_tag="VCI33_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEP2831182.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLFAKVIKSLLM"
     gene            complement(35645..36073)
                     /locus_tag="VCI33_000227"
     CDS             complement(35645..36073)
                     /locus_tag="VCI33_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEP2831183.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQDEPVSDTLDKH"
     gene            complement(36066..36473)
                     /locus_tag="VCI33_000228"
     CDS             complement(36066..36473)
                     /locus_tag="VCI33_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEP2831184.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            complement(36495..37274)
                     /locus_tag="VCI33_000229"
     CDS             complement(36495..37274)
                     /locus_tag="VCI33_000229"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEP2831185.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            complement(37271..38203)
                     /locus_tag="VCI33_000230"
     CDS             complement(37271..38203)
                     /locus_tag="VCI33_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEP2831186.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            complement(38568..38840)
                     /locus_tag="VCI33_000231"
     CDS             complement(38568..38840)
                     /locus_tag="VCI33_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEP2831187.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            complement(38904..39341)
                     /locus_tag="VCI33_000232"
     CDS             complement(38904..39341)
                     /locus_tag="VCI33_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEP2831188.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(39328..41460)
                     /locus_tag="VCI33_000233"
     CDS             complement(39328..41460)
                     /locus_tag="VCI33_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEP2831189.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            41694..41849
                     /locus_tag="VCI33_000234"
     CDS             41694..41849
                     /locus_tag="VCI33_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831190.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            41997..42899
                     /locus_tag="VCI33_000235"
     CDS             41997..42899
                     /locus_tag="VCI33_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEP2831191.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKTFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            42899..43714
                     /locus_tag="VCI33_000236"
     CDS             42899..43714
                     /locus_tag="VCI33_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEP2831192.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMVLSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLTEVHQTKEPNNPYVYRVF"
     gene            complement(43813..45237)
                     /locus_tag="VCI33_000237"
     CDS             complement(43813..45237)
                     /locus_tag="VCI33_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP2831193.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADTKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMDFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(45256..47118)
                     /locus_tag="VCI33_000238"
     CDS             complement(45256..47118)
                     /locus_tag="VCI33_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP2831194.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLTLFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFINALIAGAATIVLAFSLTWFMGIDEE
                     SPKQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            complement(47120..47962)
                     /locus_tag="VCI33_000239"
     CDS             complement(47120..47962)
                     /locus_tag="VCI33_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP2831195.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            48290..49657
                     /locus_tag="VCI33_000240"
     CDS             48290..49657
                     /locus_tag="VCI33_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2831196.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGAKLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(49711..51261)
                     /gene="ftsY"
                     /locus_tag="VCI33_000241"
     CDS             complement(49711..51261)
                     /gene="ftsY"
                     /locus_tag="VCI33_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEP2831197.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQNK
                     IEVVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHPSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATHDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KGVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(51261..52085)
                     /locus_tag="VCI33_000242"
     CDS             complement(51261..52085)
                     /locus_tag="VCI33_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2831198.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            complement(52089..52886)
                     /locus_tag="VCI33_000243"
     CDS             complement(52089..52886)
                     /locus_tag="VCI33_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2831199.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYHGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            53190..54316
                     /locus_tag="VCI33_000244"
     CDS             join(53190..53424,53424..54316)
                     /locus_tag="VCI33_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP2831200.1"
                     /translation="MSTVKRYDEEFKQSLVNLYQTGKTQSELCKDYGVSASALAKWIK
                     QYSQVKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHDTTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKI
                     EGTLLFHSDQGSQFKARKLRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KKYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEEN"
BASE COUNT        16567 a        11639 c         9894 g        16577 t
ORIGIN      
        1 ccatttttga ttctttttta gcacaaccgt tcactttttc tttttcaaaa atcttttaaa
       61 aaaagtattg acaaagaatt ttgaaagcgc tacaatattt ttgagtaaac cggtaaacca
      121 aaggagattt atttatgatt gctattatcg ttatgggaca tggacatttt gcgagtggga
      181 ttgtctcatc cctagagtta attgccggac cacaagagaa ggtaactgca attgatttta
      241 ctactgagat gaccgccgct gatgtgcagg accaattatc tcgtgcttta atcccagaag
      301 aggaaacctt agttctttgt gaccttctag gaggtacccc ttttaaagtc gctgcaacct
      361 taatggaatc tcttccaaac acgacgtgta atgttctttc tggccttaac ttagccatgc
      421 tgattgaagc tagttttgca agacaaacag cagcttcttt tgacgactta gtatcaggac
      481 ttataacatg ttctaaagaa ggcattgttg actggaaaac tctttctcag caagaagacg
      541 gtacgacaga cgatgagtta ggaggcattt aatgacacga tctttaaaaa ccattgctct
      601 tgaacctatc aagcagcctg aacgatttac caaagaagac tttctaagcc aagaagacat
      661 tactcaggcc cttgacttag ccctcaaaca agttcggctt aatatggact attttaaaga
      721 agactttcca acgccagcta ccaaagataa ccaatacgcc attatggaca acacggaatg
      781 gaccaatgcc ttttggacag gttgcctttg gttagcttac gaatacggtg gggatgatgc
      841 cattaaagct ctggctcaag caaatgacct ttctttccta gatcgagtaa caagagatat
      901 tgagctagat caccatgatt tgggctttct ttacacccct tcttgcatgg ctgagtggaa
      961 acttttaaaa acacctgagt ctagagaagc tgctttgaaa gcagccgaca aattaatcca
     1021 acgctatcaa gacaaaggcg gctttattca ggcctggggg gtgctgggta aaaaagaaga
     1081 ttaccgttta atcattgatt gtctcctaaa catccaatta ctcttttttg ctagccaaga
     1141 aactggagac aaccgttacc gtgacatggc gatcaatcat ttctacgcct cagccaatca
     1201 tgtcatccga gatgacgcct ctgcttacca caccttctac tttgatccag aaacaggcga
     1261 tcctattaaa ggggtcaccc gacaaggtta tagcgatgat tctgcttggg ccagagggca
     1321 agcttggggc atttatggta ttcctttgac ctatcgtttc ctaaaagaac ctgaactcat
     1381 tcaactcttc aagggcatga cccattattt catgaaccgt cttcctaaag accaggtttc
     1441 ctactgggat ttaatttttg gagatggtag cgagcagtct cgtgatagtt cagcaaccgc
     1501 cattgctgta tgtggtattc atgaaatgtt aaaaacctta ccagatcatg atcctgataa
     1561 aaaaacatat gaagctgcca tgcacagcat gctaagagcc ttgattaagg attatgctaa
     1621 caaaaatctc aatcctggcg ctcctctact acttcacgga gtctattctt ggcactctgg
     1681 aaaaggggtt gacgagggaa atatctgggg agactattac tatcttgaag ctctgcttcg
     1741 tttctacaaa gactggaacc cttactggta acaaacaccg ataccgacat ttaaaaagga
     1801 gattttatga cacaaccaaa cattatcatg acgcgagttg atgagcgctt gattcacgga
     1861 caggggcaat tatgggtcaa attcctcaac tgcaacaccg ttatcgtcgc taacgatgcg
     1921 gtttctgaag ataagattca acaatccctc atgaaaacag ttattccatc cagtattgcc
     1981 attcgttttt tctccattca aaaagtgatt gacatcattc ataaagcaag tcctgctcaa
     2041 agtatcttca tcgtggtcaa agatttgcaa gatgccaaac gcctagttga agggggtgtt
     2101 cccatcacag aaatcaatat cggtaatatt cataaaactg atgataaggt tgcgattacc
     2161 cagtttattt cgctgggaga aacagacaag tctgctattc gttgcttagc ccatgaccac
     2221 cacgtggttt ttaacaccaa aacaactcca gctggcaata gcgcctctga cgttgacatc
     2281 ttagattata tttaatgaat aaaggagatt gatatggata tcaatttgtt acaggcactt
     2341 ttaattggtc tttggacagc cttttgtttc agcggaatgt tacttggcat ctacaccaac
     2401 cgttgtatta ttctgtcttt tggtgtaggg atgatcttag gtgacttgcc aactgcactt
     2461 agcatgggag ctatctccga attagcttat atgggatttg gagtgggtgc cggtggtacc
     2521 gttcctccta atcctatcgg ccctggtatc tttggtacct tgatggctat taccagtgct
     2581 ggtaaagtta ccccagaagc ggccctagcc ttatcaacac caatcgcagt tgtcatccaa
     2641 tttcttcaga cattcgccta taccgctttt gctggggctc ctgaaaccgc taaaaaacaa
     2701 ttgcaaaaag gcaatattag aggcttcaaa ttcgctgcca acggcactat ctgggctttc
     2761 gcttttatgg gattaggcct tggtttatta ggtgccttgt caatggatac cctccttcac
     2821 ttggttgatt atatcccacc tgtcttgctc aatggattga ctgttgctgg taaaatgtta
     2881 cctgctatcg gatttgccat gatcttatca gttatggcca agaaggagtt gattcctttt
     2941 gtactgattg gttatgtttg tgcagcctac ctccaaattc caaccattgg tatcgccatt
     3001 attggtatca ttttcgcctt gaatgaattt tacaacaaac ctaaacaagt cgatgcaaca
     3061 actgtccaag gaggccaaca agatgactgg atctaataaa ttaaccaaga aagattacct
     3121 caaaacagct ctgagagctt tcttcctaca aaatgggttt aattacagta attaccaagg
     3181 tatcggatac gccaatgtca tctatcctgc cttgaaaaaa cattttggca gtgataaaaa
     3241 gggcttatat caagctttag aagataactg tgagttttac aatactaacc ctcacttcct
     3301 tcctttcatc accagtctgc acttggtcat gttagaaaat gatcgtcctg aagaagaaac
     3361 ccgaaacatc aaaatggcac ttatggggcc tttagctggt atcggtgact ccttatcaca
     3421 attttgcttg gcgccactct tttcaacgat tgcagcttcc ttagctagtg atggtttagt
     3481 gttaggtcca attttcttct tcctagccat gaatattatc ctaactgcta ttaaaatcgg
     3541 ttctggtctc tatggctaca aggtcggcac aagctttatt gataaactaa gcgaacaaat
     3601 ggcgatagta tctcgtatgg caaatattgt cggtgttacc gttatcgctg gattagctgc
     3661 tacttctgtt aaaataactg ttcccattac ttttgcggct ggcaaggtgg atgcggccaa
     3721 tacagcacaa aaatttgtga ctattcaagg aatgcttgat aagattgccc ctgcactttt
     3781 accagctctc tttaccttac tcatgtatta ccttattaaa aacaaaaaat ggacaaccta
     3841 caaattggtc attttaactg ttatcattgg tgttattggc agttggttgg gtattttagc
     3901 ttagttgtca ctgagattct gttatgggtt taggaactat ttcctagtca cattaacagg
     3961 tctcttttct tttggaaagg aaacgtccta tgtctcttgc cttcacacgc tttaaagaaa
     4021 cagtcaatcc aaacttttgc aggaattacc tgctagacta tcaggctgac agctatgctg
     4081 atcgaaaaag gatagctgac ctactcttaa ccaatacctt tttatttgaa gataattggg
     4141 atatggagcc ctgccacatc ccttaccact tagatcccat tacttggcaa gaggctgtca
     4201 cagatgatcc tgagtggaat tttatgttaa atcgacagac ctatctccaa acattaatcc
     4261 tcgtctacct tgttgaaaga gatgaacggt atctcttaac agctaaaggt ttcattctca
     4321 attggattga atcagctatt cctttagatc ctaaggggtt agctactaga accttagata
     4381 ctggcatccg ttgttttgct tgggtcaaat gtttgattta ccttaatctt tttgacgcct
     4441 taaccaagca agaggaaagg ctcatcctag cttccctgga aaaacaactt cactttctcc
     4501 atgccaacta ccttgataaa tacagtctca gcaattgggg aatcttgcaa accactgcga
     4561 ttttattagc tgatgcctac tttggttctg accttgatat tgctgcagca acagctttcg
     4621 ctagaaaaga attgacgcaa caaatcgcac tccaaattct cgaagacggc agtcaatttg
     4681 aacaatcaac catgtatcat gtggaagtac tcaaagccct tctggaatta actgctctag
     4741 tccctgatta tcttccacag cttagaccta ctttgcttgc catgagtgat tatctcctca
     4801 aaatgactgg ccctgaccac aaacagatcc ccttaggaga cagtgacttt accgatacca
     4861 gagatattct aacactagca gccactatcc ttgaagaacc acacttaaaa gctgctgctt
     4921 ttcccacact tgatatcgat agtcttctcc ttctaggaga aaaaggtgtc cacacttttg
     4981 aacagttacc cgtccaaacc ttaccaactt ttgcgcatca ctttgaacat tccgggcaca
     5041 ttaccatcaa tcaagaaaat tattatcttt ttttcaaaaa tgggcccatc ggtagttccc
     5101 atacccatag cgaccaaaat agtatctgtc tctattataa aggacaacca cttttctgtg
     5161 atgctgggcg atacacttac aaagaagaac cattacgcta tgccctaaaa agcgctagcc
     5221 atcattctac cgccttctta gaagaacaat tacctgagca gattaatagc tcctgggctt
     5281 acctttctta ccccaaatcc aattattgtc accttcgaca aaatggacac gtttacttta
     5341 ttgaaggtag ctaccagacg caattctctg ataggaacaa ctaccaacat gaccgacaaa
     5401 ttctcatctt accaccagga atgtttctta tcattgatac gatacaggct caaggtaacc
     5461 actgtcttgt cagtcaattt attcttgata atcacttaga agtaaagaca gatcacctta
     5521 gtgacctcag actaatcagt gactgccctt tcactatcga agaaacgatt ttgtcaaaaa
     5581 aatacaacca atacctgact agtcacaaat tcatcaaacg aaaacccttt aaagacaagg
     5641 gttgcaccag taccctgtta gtccctgacg ataccaaagt tactccctta acaccacttc
     5701 aaacaggcaa acgcaaccca attgagaccg ccctttcctg gcatttaaaa ggaaagcagt
     5761 ttgactacag tatctgcgtt ctccaagagg acttaattaa aggagaaaaa ctagttctcc
     5821 taaatggtca taaaatcaga ggaaaggtgg ttgttatcaa ccatatcacg aatgagatta
     5881 ttcgattgaa acactaaatc ttttacgagt ccttatgtta taatatgact aaccattact
     5941 attttaaagg agtgtatgca atgcagcgca aagtaaccat caaagatatt gctgaattag
     6001 cgaaaacttc aaaaacaaca gtttctttct acttaaatgg taggtttgac aagatgtctg
     6061 aagaaactaa aaaccgcatt aaaagcacga ttgaagctac caattataaa ccaagcattg
     6121 ctgccagaag tctaaacgcc aaatctacta aactcattgg cgtggttatt ggtgatatta
     6181 ccaatagttt ttccaatcaa attgttaagg gaattgaaag caaagcccag gaatttggtt
     6241 accaaattat catcggaaac agcaactacg acccttctcg cgaagatgaa ctcattgaaa
     6301 agatgctgaa cctcggagtg gatggcttta tcattcagcc tacctctaat ttcaggaagt
     6361 attctcgcat tatcgatatt aagaagaaaa aagtggtctt ttttgatagc caactttacg
     6421 aacatcgcac aaactgggtc aaaactaata attatgatgc tgtttatgat accattcaac
     6481 aatgtatcga taaaggttat gaacatttta tcatgatcac gggtaacccc aatttactta
     6541 gcactcgtat tgaacgcgct tcaggcttca ttgatgtgtt agaagctaat catcttactc
     6601 atcaagaaat gattattgat gaaaatcaga cttcttcaga ggcaatcgct cagtttttgc
     6661 aaggatcact gactaaaaag agtttggttt ttgtccccaa ttgttgggct cttccaaaag
     6721 tatttacagc tatgaagtct cttaaattta atataccaga gattggacta gtaggatttg
     6781 ataacattga gtggacaaaa ttttcttcac caacactaac aactattatc caacccgcct
     6841 atgaagaagg cgaacaggct actaagattt taatcgatga tattgaaggt cacagtcaag
     6901 aagccaaaca acaaattttt gactgtcaag tcaattggca agaatcaact ttttaatgtc
     6961 aggcatacca atagtcctta tcgttgtaac gattataaaa aaaggtttga gacaattgtc
     7021 tcaaaccttt tagcgtctat taaattgctt tttcatcttg accaagtgat cgttgaacag
     7081 ctttcaccaa ttcaacaaga acaaccatca agagacttcc gataataacc actaaccatt
     7141 gtgtgatcgt caagtatgtc acatggaaga attgattaaa tccaggaaca acaactgttg
     7201 ccatcaatgc tacaaaggcc actggaatag aatagttgaa caatttgttt ttgaacaacc
     7261 caacagtaaa gatggattgg taaacagatt tcacgttgta ggcatgtacc aactgaatta
     7321 aaccgagggt cacataagcc atggtcaagg catctgcatg gacatcatgg tagcttgtgt
     7381 gttctgggaa catcaaggca aaaccataaa ccccaagcac caaaatggtt tggaaggcac
     7441 cttggtaaag aatggcttcc ttcaccccac catcaaagaa gcttgactta cgtccacgag
     7501 gtttgtgttt catcacacca ggctcagcag gttcaacacc aagagcaata gctggtaggg
     7561 tatcagttac caagttaatc caaagcaagt gaactggttg aagcacatcc caaccaaaca
     7621 aggttgcaag gaagatggta aagacttctg ccatgttagc agaaagtaaa tattgaattg
     7681 ttttttgaat atttgagaag accttacgtc cttcttcaac cgcaacgata atggttgcaa
     7741 agttatcatc ggcaagaacc atgtcagaag ctcccttaga aacctctgta cctgtaattc
     7801 ccataccaat accaatatct gccgttttaa gagatggtgc atcgttaacc ccatcgccag
     7861 tcatggcaac aactttgcct tcattttgcc aagccttaac gatacgaact ttatgctcag
     7921 gagatacacg ggcatacacg gagtattgtt taaagacttt ttggaactct tcatctgata
     7981 attcgttgag ttcagcacct gtaaagacat gatcaacgcc atcttcttcg ataataccaa
     8041 gacgtttcgc aatagctttt gctgtatctt ggtggtcacc agtaatcatg attggacgaa
     8101 tgccagcttc cttagctact ttaacagcct gtgctgcttc aggacgttca gggtcaatca
     8161 tccctaccaa accagagaag acaagattag cctcaacaat ttctgtttcc agtgttggaa
     8221 gagcatcaga atatttgtaa gccatcatca agacacgaag agcttgtttg gcaagactct
     8281 tattggtatc aagaatggtt tttttatcag catccgtaat aggacgaatt tgtccatttt
     8341 cttcaatctg tgtgacacgc ttcaacaatt ggtctggcgc acctttaaca gcgatgaaat
     8401 atttgccatc agcttgttta tgaatggttg acatcaactt acggtcagaa tcaaatggta
     8461 attctgccac acgaggttct gccaccatag cctcacgtac gtcaaaattg tggtctaacc
     8521 caaactgtac caaagctgtt tctgttgggt caccaattaa cttccctgaa ggatcaactt
     8581 tagtatcatt agcaaagttc attacacgaa gtgtcgtatt gtcaaaagca atatcagctg
     8641 atgaactttg gagtgtcccg ttggtataaa ctttttcaac ggtcatttga ttcattgtca
     8701 aggtacccgt tttatcagaa gcaatgattt cagttgatcc tagcgtttca acagctggta
     8761 atttacggat aatggcgtta cgtttagcaa gtacttgagt tccaagagaa agcacaacag
     8821 taacaatcgc aggaagtccc tctggaatag ctgcaacggc aagcgctact gaagtcatca
     8881 agccttcaag cgggtgttga ccacgcaaga agactccaac agcaaaagtc accgctgcaa
     8941 tcacaaggat cgcataagtc aaaatcttag aaaggttgtc aaggttttgc ttcaatggtg
     9001 tatctgtctc atcagcatta gcaagcatac cagcaatatg cccaacctcg gtgtacatgc
     9061 cagtgttagt gataacccca atcccacgac catatgtcac atttgagttt tgatatccca
     9121 tattgacgcg atcaccaata ccggcatctt ctgaaacagc cgttgacaag tctttttcaa
     9181 ccggtacaga ttctccagta agcgctgctt cttcgatttt taaggagtta gcttccaaaa
     9241 gacgcagatc agcagggaca acatctcccg cttctagcaa gacaatatct ccagggacca
     9301 attctttaga gtctatctca gtcacatgcc catcacggcg aatacgagcc agtggacttg
     9361 acattgattt caaggcttca atagcagctt cagcttgtcc ttcttggtaa acaccaaaag
     9421 cagcatttag gataacaaca gccaagataa taatggcgtc tgtcaaacct tccatcccct
     9481 ctgtaagaac tgaaagaagg gctgccacaa tcaaaataat aatcatcaaa tccttaaatt
     9541 gatcaaggaa tttcataaat aaacttcgtt tttcgccttc atcaagctca ttacgaccat
     9601 actctgctaa gcgctctttt gcttgggcag acgtaagacc ttcgcgactg gtctctaatt
     9661 gagccagtac ggtctcttca ctttgcgtgt aaaatgcttc atgtctttgt tctttagaca
     9721 aattagtacc tccttggtct ttttatttta gttgttcaaa taaaaagacc tgtttatttt
     9781 cttaaacaag tctcgctgtt taagattgta ccggaaaccg aaattccgta ttgacgatac
     9841 aatcacggct agccgtctgc tactcccttg atataataac cattatagta aaaacctgac
     9901 taaaaaacaa gaataatccc taatcagatg atttactgct atctttttag ttaatcccaa
     9961 ctcagctcaa gatgatagct tgcaaatagc atttctgttt gagcttgaag caaatgataa
    10021 tgaaggtcca atttttgaca agaacaatct aagtggtaat gactggtatc tgtcaaaaat
    10081 tgttgaaccc ccatcggtgt aggtagagca atcaggacct tcttgcccgc aaaaaatttc
    10141 atgattgact gagggttgga aaaacggctc atggtcagtt ctgtctcatt atacttgatg
    10201 acaacctttt ccttgtcagt gttttgatag atgaggtact gatagccccc tttttctcgc
    10261 cactcacaat catgaatttc ttgaataatc tctgtctcat ctccaaagcg aatatggttg
    10321 gttaactgta atttcataac tcttctttct attctaagct cgtctgttta ttctatcaat
    10381 ttctccctat tttatcaatt tttgatataa taaaaattat gaatgaaaaa gtatttcgtg
    10441 accctgttca caactatatc cacattgata atccattaat ttatgacctt atcaatacca
    10501 aagaattcca acgcttacga cgcatcaaac aagtccctac aacagctttc accttccacg
    10561 gcgcagaaca tagccgtttt tctcactgcc taggtgttta cgaaattgcc cgtcgggtga
    10621 cagctatttt tgaagagaaa tacgctgata tctggaataa agatgaaagt ctggtcacta
    10681 tgacggctgc cctcttacat gacattggtc atggagccta ttctcatacc tttgaggttc
    10741 tttttcatac ggatcacgag gcctttactc aggagataat taccaatcct gagacagaaa
    10801 tcaatgccat tttggtgcgt catgctcttg attttccaga caaagttgcc agcgtgatta
    10861 atcataccta tcctaacaag caagttgtgc aactgatttc cagccagatt gactgcgacc
    10921 gtatggatta ccttttaaga gattcttatt tttctgctgc caattatggt cagtttgatt
    10981 taatgcgcat tctacgtgtg attcgacctg ttgaagatgg cattgtcttt gaacatagtg
    11041 gtatgcatgc tgtcgaggac tatattgtca gtcgttttca aatgtacatg caggtctatt
    11101 ttcatcccgc cagccgtgct gttgagctca tcttacaaaa tctcctcaaa agagcccaac
    11161 acttatatcc tgagcaacag gcttatttcc aaaaaacagc tcctggactc atccccttct
    11221 ttgaaaagaa ggctaattta gcagattata tagccctaga tgacggagtc atgaatactt
    11281 acttccaagt ctggatggca agcgaagacc atatcttatc agatttggcc agtcgcttta
    11341 ttaatcgaaa aattctgaag tctgttacct ttgaccaaga ttcccgagga gagttagaac
    11401 gcttgcgcca attggtcgaa tcagttggct ttgatccaga ttattacacc ggtatccata
    11461 tcaacttcga cctgccttat gatatttatc gaccagagct cgaaaatcca agaactcaga
    11521 ttgaaatgat acaaaaagac gggagccttg ctgaattatc tcagttatct cctattgtca
    11581 aagcattaac agggacaacc tacggagacc gtcgctttta ttttccaaag gaaatgttag
    11641 agctcgatga cctctttgct cctagcaagg aaacttttat gagttatatt tccaatgggc
    11701 attttcactt tagtcagtaa agccctttaa gattgcagaa aaagtctttt ttgatacttt
    11761 tcttaggtga tgcttatggc agcgacttcc tttggaattc tatgctttat ttttgagcct
    11821 agggtctcaa aaattccgcg taacgtcact gccagtattg acaccacttt tgccacagca
    11881 aaaagcagct gttttggctc acgtcacacg cagaaataag aaatcagttg tcaaatcagc
    11941 agagcagttc atgctttttg agcatcaaac tgggtttgtc tccattctaa gattgaagaa
    12001 agagtacttt cttcaatctt tttagttact acagaggcga ttgagctaaa ctaaaacgtt
    12061 ttctctcgtt tttccttaag tttcctttct catcccatga caaaagcacc tatttttagt
    12121 aagataggag ggagaacatt tttaagataa accacaaaaa aacaaggagg atgccatgtc
    12181 tatcaaactg gttgctgtcg atattgatgg cacattactc actgatgacc gtcgaattac
    12241 agatgatgtt tttcaagctg tccaagaggc taaagcccaa ggggttcata tggtgattgc
    12301 tactggcaga cctattgccg gagtgatttc attactcgaa caattagaac tcaaccacaa
    12361 aggaaaccac gttatcactt ttaatggtgg cttggtgcaa gatgccgaga ccggcgaaga
    12421 aattgttaaa gaattgatga cttacgacga ttatctggaa acggaatttt tgagccgaaa
    12481 attaggagtt cacatgcatg ccattaccaa agaaggtatt tacactgcca atcggaatat
    12541 tggaaaatac acagttcatg aatcgacttt ggtaaacatg cctattttct atcgcactcc
    12601 cgaagaaatg accaataaag aaatcatcaa aatgatgatg attgatgaac ctgacctttt
    12661 ggacgcggcc atcaagcaaa ttcctcaaca tttctttgat aagtacacca tcgtcaagtc
    12721 aacccctttt tatttagagt tcatgccaaa aaccgtcagc aagggcaatg ccatcaaaca
    12781 cttagctaaa aaactcgggc tagatatgtc acaaaccatg gccatcgggg atgctgaaaa
    12841 tgatcgtgct atgttagaag tcgttgccaa ccctgttgtc atggaaaatg gggttcctga
    12901 attgaaaaaa atagccaaat acatcaccaa atccaacaat gacagtggtg tggcacatgc
    12961 tattcgcaag tgggtattaa actaatgtat acatataaaa tcggaatatc tcctgaagaa
    13021 catgatcagt ttgtcttagc acagccacaa gctggcttac ttcaaagttc aaagtggaga
    13081 aaagtcaaag ataattggaa acacgaacgc atcagctttt atgaaaatgg ggtacaagtt
    13141 gctgctgctg cttgtttaat cagaaaactt cctcttggtt tcactatgat ttatatccca
    13201 cgtgggccaa tcatggacta cgccaatttt gaactccttg atttcgtcat taaaacgctc
    13261 aaaactttcg gaaaaagcaa acgagcgctt tttgtcaaaa ttgatccaag tttagtcatc
    13321 aaacaaactt tagaaggaaa agaaagcaaa gaaaatgatg tgacactctc tattattgct
    13381 tttctaaaaa aacttggtat cgaatggtct ggcagaacaa aagaactcga agacactatt
    13441 caaccaagaa tacaagccaa catctacgcc aaggattttg actttgacag cctgcccaaa
    13501 aaagccaagc aatccattcg taccgctacc aataaaggtg ttaatgtcac gattggtggc
    13561 agtgaattgc tagacgactt ttcagccttg atgaaaaaaa ccgagaatcg aaaaggcatt
    13621 atccttcgag gcaaatccta ctatcaaaaa ttactagata tttatgctgg tcagtcctac
    13681 attactatgg ctagtttaga cttacctgaa caaaaaaaac tcttgatcca acagttggac
    13741 aaggccttag cagaacaagc acgattaaca gataagagta aacctagcaa agtagcagaa
    13801 aatcaaaaaa ccattgcacg gctacaaaaa gatttaacca ttttaagcga gcagctcgct
    13861 gcaggcaaaa cccgcatccc actagccgcc accctaacct tgatttatgg cgagacttct
    13921 gaaaatcttt atgcgggaat ggacgatgat tatcgcaatt atcaagctcc tcttttaacc
    13981 tggtatgaaa cggctaaaga agcttttaaa agaggctgcc gctggcataa tttaggaggt
    14041 gtggaaaatc aacaagatgg tggactctat catttcaaag ctagactcaa ccccaccatc
    14101 gaagaatttg caggtgaatt caacattcct gtcggccttg tttcaagctt agccatcttg
    14161 acctataact taagaaaaaa attaaggagc acaactaatg gcactaattg aaatttcaca
    14221 agaacaattt gaccattact gccacagtct cgtccatcat tcctttatcc aaacctcaga
    14281 aatggccagc ttgatggcta aaagaggggc aaaaccacaa tttcttggac ttgaaaaaga
    14341 tggtgaacta aaggttgctg ctatggtctt tagccaaaaa gtggctggag gttggcgtat
    14401 ggaactcaat gcaggcccta ataccaatca tcccgaagaa ttagaacatt tttacactca
    14461 acttaaagac tatgccaaac aaaatgacgt tatcgaactc attttaaaac cttacgacaa
    14521 ttatcagtca tttgatactg atggcatccc tatttctcat ccaaacactg accttattag
    14581 ccttttaacg gcgttaggat ataaacatga cggtttaaaa acaggctatc ctgagggtga
    14641 acctgtttgg cattacgtta aaaagttaga aggaattgat tcctcacgtt taacccattc
    14701 cttcagcaaa aaaggaaaag ctttaataaa aaaagctaat acttttggta ttaaattacg
    14761 tcaactgaaa cgagatgagt tacaccattt caaagaaatc actgaagcaa cctctgatcg
    14821 tagagattac cttgacaaat cattatctta ctatcaggac ttttatgata gttttggaga
    14881 ctcttgtgaa tttatggttg cgactcttaa ttttgaagat taccttaata accttaaaca
    14941 aagacaacta caactagcaa ccagcatcaa caaagttaaa ggagaccttg gcaaaaatcc
    15001 tcattctgaa aaaaagcaaa atagattaaa agaactatcc agccaattcg aaacctttca
    15061 ggtaagaatt tctgaagctc tccattttct tgaagagtac ggaactaaag atgtgttcct
    15121 tgcgggaagt ctttttatct ataccgaaca agaggccgtc tacctctttt caggatctta
    15181 ccccaaattt aataaatttt atagcccagc tctattacaa gagcatgcta tgctcaaggc
    15241 aattcacaag ggcatcaaac aatataattt tcttggtatt actggaaagt ttgatggttc
    15301 agacggtgtc ttgcgcttta aacagaattt caatggcttt attttgcaaa aaccagggac
    15361 atttagatgt tatccatttc caatcaaata tcatttcatt agattggcaa aaaagctatt
    15421 aaaccgctat taggctaatg catttgcctg tagaatcacc tttattctct catagtaaga
    15481 gttaagtcat ctaaaaaaga aaaggattta accttttctt ttttagatga cttagttaag
    15541 gaaatcaagc aaagcaagga agctatcagc ttcaagtgat gcaccaccta caagggcacc
    15601 gtcaacatct ggacaagcca tgtaatcttt aacgttttca ggttttacag aaccaccgta
    15661 ttgaacgcga actttatcag caacttcttg cccaaagtct gctgctacca cgtcacgaac
    15721 agctttacac atgttttgag catcatcttg agtagctgat ttaccagtac cgatagccca
    15781 gattggctca taagctaaaa caagtgaagc tacttgctct gctgaaagac ctgcaagtgc
    15841 tgctgaaact tgcgcaccta caaactcaac ggctttacca gcttcataag tttcaagaga
    15901 ctcaccacaa caaacaattg gagttaaacc gttagcaaag attgctttgg cttttttatt
    15961 aatgtcttca tctgtttcat ggaagtaatc acggcgttct gagtgaccaa tcacaacata
    16021 gtcagctccc atttcagcaa gcacttttgg tgatgtttca ccagtgaatg cccctgtgtt
    16081 ttcaaagtag cagttttgtg cagcaacttt aagaactgaa tctttagcag cttcaatagt
    16141 ggttaccaaa tcaacagctg gggctgctac ggcaacatca acaaggtcag ttgaaggtaa
    16201 tttgcttgct acagcctcaa cgaatgcttt tgcttcttga gggtttttgt tcattttcca
    16261 gttaccagca ataattggtt tacgtgacat atcacatacc tcttcttttt ttatttatat
    16321 cttctatttt agcacaaatg accgacgatt aaaaggaatt gcttatattt taggaatatt
    16381 ttcacaaata aaaacccttt ttctgaaagc ttttcattct tatttatcag cattttatgg
    16441 agtttaagat agcaaaaagc ccccgaattg ggggcttctt caaacttaag ttgtctgact
    16501 taaattgcta ttctgggaac ttaattaaaa ttaagcttcg atttctgaaa cgatacctga
    16561 accaacagta cgtccacctt cacggattga gaaagtagta ccttgttcta cggcgattgg
    16621 gtggatcaac tcaacgttga ttgtcacgtt atcaccaggc ataaccattt ctgtacctgc
    16681 tggaagttcg attgaacctg ttacgtcagt tgtacggaag tagaattgtg gacggtagtt
    16741 gttgaagaat ggagtgtgac gtccaccttc gtctttagaa aggatatata cttcaccttt
    16801 gaatttagtg tgtgggttga ttgaacctgg tttagcaata acttgaccac gttcgatttc
    16861 gtcacgttga acaccacgaa gaaggatacc tacgttgtct cctgcaagac cttcgtcaag
    16921 ttgtttacgg aacatttcaa caccagtaac aacagctttt ttagtttctt ctttgatacc
    16981 aacgatttcg atttcgtcgt tgacacgaac agtaccacgg tcgatacgtc ctgaagcaac
    17041 tgtaccacga cctgtgattg agaatacatc ttcgactgga agaagcaatg gtttgtcagt
    17101 gtcgcgttct ggttctggaa tgtatgaatc aacagtatcc atcaattcca tgatgatgtc
    17161 ttcaaattta gtgtcgcctt caagagcttt aagagctgaa ccttggataa ctggaaggtc
    17221 atcacctggg aaatcgtatt ctgaaagaag gtcacgaatt tccatctcaa ctaattcgag
    17281 caactcttcg tcatcaacaa ggtcaacttt gttcatgaac acgataaggt gtttaacacc
    17341 aacctgacgt gaaagaagga tgtgctcacg cgtttgtggc attggtccat ctgttgaagc
    17401 aactacaagg atagctccgt ccatttgagc ggcaccagtg atcatgtttt taacgtagtc
    17461 cgcgtgtcct ggagcgtcga tgtgcgcata gtgacgagtt gcagtttcgt actcaacgtg
    17521 tgcagtgttg atagtgattc cgcgttcgcg ttcttctgga gcagcatcga tagaagcgta
    17581 atcttttggt tggttaactg atgaaggcaa gcgacgtgcc aatacagttg tgattgcagc
    17641 tgttaaagta gttttaccat ggtcaacgtg tccgattgta ccaatgttaa cgtggggttt
    17701 actacgatcg tatttttctt ttgccatttt aggaaaagcc tccaataaaa tatattttat
    17761 agatagacgg taggccatac agtctaactt taccttacta ttgtagcaaa ttacaatgaa
    17821 aatgcaagta ttttatgttt tttgccattt ctttattttt taaatagaag aaaaagtctt
    17881 ttttgatact ctccttaggt ggtacttata gcagcgactt cctttggaat tccacacctt
    17941 atttttgcca cagcaaaaag cagctgtttg ggctcatgtc acacgcaaaa ataagaaatc
    18001 agcagagcag ttcaagtttt ttgagcatca aactgggatt gtctatattc taaaatctcc
    18061 ttaaaggaga ttttagaata ttattgaaat tgcttgatat ttacaacttt actattttct
    18121 tttcgagtgt cctttcgata agataattca gcttctttaa atatatcatc tcgcttttcg
    18181 ctagcatcaa tatttagtac aaaacccact gcaacagata agacaagtaa gctatttcca
    18241 ccttgagaca aaaaagggaa ggtaacacca gtagatggaa ttaagcctga gataccacca
    18301 atattaacaa agacttgcat gagcatcata ccgcctactc ctaaagccat catggcatta
    18361 aaaggatttt ttgctttaat accaacattc ataatacgta aaattaagaa gaaaacaagt
    18421 gccaaaataa accctgctcc tattagacct aactcttcaa tgacaactga aaagacaaag
    18481 tctgtctgtg cttcgggcaa atagccacgc ttttcaatgg aatttcctaa cccttggcca
    18541 aaccacccac cattactcat agcatagtaa gaattggcta attggtgacc tgaatcggtc
    18601 aagtcatgaa aaggattaaa aaaggcacta aaacgtttag cgacatagcc aaaaacagga
    18661 atcttagcaa ctctttctac cccaataacg gcaatagtcc caagaaagac cgtagataga
    18721 ccggtaatca tgactaggat agccgaaaac catcggtaac caatgccact gatagaaaac
    18781 ataataatgg ctgtcaatac aataatggaa gcatttccta agtcaggctg agcagcaacc
    18841 aaaagtacca tcaacaaaga ataaacacgc cagtcccgta aatcgttcca ttgtgttggc
    18901 caccattttc gacgagttag tgcttgataa tcataaaggc taatgttttt ttggatctta
    18961 gcaaaggtca atgccaaata ccataccata atgatcttta aatactcagc aggctgaaag
    19021 ctgactggtc cgatcacaat ccaaccatgc gctcctttga tagcagttgt aaagaagcgg
    19081 gcaataatta aaagaaaggc ctctcctaac ataaccacag ttaataccct tgtattcgtt
    19141 aaaaaattta attttaattt atagataaat gtaatagcca ctagacttat tatccaaaaa
    19201 actccctggt taatcaccga tttaaatgga ttagcatgtg cttgaatcaa gctgacactt
    19261 gtggttgagt aaaccattat taagcctatc accgataaaa ttaaataagg caataatata
    19321 gaataattta agaggtgcct tttatcaatc ttcatagttt tccctctact tcttgagata
    19381 atcatttagc attataacat ctttctatat caaacaaaag aaaaagtcaa gaatcctcaa
    19441 aaaaagaagc tccaagggct aacctttaaa attctgatta gtcacttgaa aacttctata
    19501 aataccaatt aatactcaag agcattagcc tgaattacgt aaaccagtcg ctatgccatt
    19561 gatagttgta tggatcaatt tttcatcatt ttcgtcaagt tgattattac gccaacgttt
    19621 aatcaattca atttgaatat agttcaacac attaaaatac ggcaagcgtg acttcaagct
    19681 atgcttaagg gaaggattat cctccaatag atcatcatgg tcttggatag caagaataac
    19741 attttttgtc aattgccact catccaaaat ttcataaaaa acatctcgaa catcctgact
    19801 ttcagctagt tgtgcatact gaaaggcaat attcatgttt gatttagata ataccatatc
    19861 aacatttgat aacaaggaat ggaaaaaagg ccacgtctga tacatgtgtt ggaggcgttc
    19921 aaggttacct tgtgctcggt caatataacg tttaaaggct gaacctaccc cataccaacc
    19981 tgggaacatg atgcgatttt gtgaccatga aaagacccaa ggaatggcac gcaagcctgt
    20041 aatttccgta atcgttttac gggctgctgg tcttgaccca atgttgagac tggaaacttc
    20101 cttaatcggg cttgcctcaa agaaataatc atagaaatgg ggattgtcaa aaaccaattt
    20161 tcgataaatg gtattgctat cagcaacaat gctatccatg atttctcgaa aactatcaat
    20221 ctcattagga tctgttatca tttgagtcac catacgattg atagaagctg aaatcaacat
    20281 ttctaaatgg taataagcca catctttatt gccgtatttg ttttcaataa tttccccttg
    20341 ctctgtcaga cgaatccgat ctttgatgga accaaaaggt tgtgaagtaa tggcatcata
    20401 agaagggcca cctcctcgac caactgttcc tccacgacca tggaagaagg tgattttcac
    20461 tccatgttcc tcaccaatag ccgttaattc attctgagct ttataaagag tccaacccga
    20521 tgccaagtag ccaccatctt tattactgtc agaatagcct aacatgattt cttggtaatt
    20581 acgattcgtt gcaatccaag atttgacaat gtcatggctt aaataggctg ccatgatgtc
    20641 acgggcatta tctaaatcct caatggtttc aaagagggga acaatttgca cacgagcccg
    20701 ctgctggtca accaatccca cttctttaag cataatggcc aactcaaaca tatcagagac
    20761 actttctgta tgtgaaataa tatgttgctt tataacctct tctccaagct tatctttcaa
    20821 ttctcgtgcc gtagtataga tagccagttc tttttctaat aggtctgatt tagcaactgc
    20881 cgctgaggat aaagtccttg gctcctccat taattgttgt aagagaacat cgcatttttc
    20941 agtttctgaa agagagctat aatcgtcaac aatattggct ccttttaata actctgccac
    21001 acaagattct tggacactgg agtcttgcct catgtcaatg cttgctaaga agaagccaaa
    21061 aatatcaaca gcttgcatga cttctcgcaa atccccctca atcaaagcgg agttgccatt
    21121 atcaagtagc gactgctcaa tagcctttag atcagcttta aaatcaacag gagagtcata
    21181 ggctaagatg ggattttcta aactagctgg tgaagaccaa gcagatgtgc ttagtcccac
    21241 actactactc atacgagctg ctggctgatt agttagttga atttgtgttt gtttaagacg
    21301 cgattgaata taatgaaaag ctctgcgata aggttcattt ccacggtaaa tcgattggtc
    21361 ctgagacaaa ctggctaaac gttccacttc cgagttgggt tgtaccaagg tcgacgacag
    21421 tgaaaatgtt cggtaaaggg ctgctaattt gtcaatgtag taattgagga taacctcact
    21481 ttgaaccgta gctgacaagc acaaggtttc agctgtgaca aaggggttcc catcacggtc
    21541 cccaccaatc cacatcccca tggtaatggg tttgggattg tctaactcta gaccgtgttt
    21601 ttgggctaag tttttgtact ctgttgtcaa cttggtcact gcttgaatga gagaaccatc
    21661 gtaatactgc atgacatttt ttatctcgtt tttcacctga agctttttct cgcgaataat
    21721 atctgtctgc atgatcattt caatataacg atatagctcc tgacgccatt tctcaagatt
    21781 aatcacacca gccttagcat cacggtattt gcgcagaagt ttatgaatgt gagttgtcaa
    21841 ttccagaatc gttttacgtt gtacttgcgt cggatgtgct gttaaaacag gaaccacatt
    21901 aacctgctcc aaaatgtctt taccattatc ttttccagca agatccttga tggttagggc
    21961 taattttcct aaataatcag tatccgtatt attttgataa ttgatttcat aggctaaatc
    22021 gacatcttct gaaatattga ttagcaaggg taaaatagaa aaatacctgg aaatcacttc
    22081 catttcttga tttgaaatat tggcgacaac tttttctaat tctatgtaat cttgtttttc
    22141 agataacacc ataatagact cgataactgc aaacgcatca tctccaatca tccgtcgtgt
    22201 gatattttcc aacatttctt tcaaaagagc aacttcttca gcaatgatag cttgattgtt
    22261 actactctct aactttttaa gtggcaaatg accatctcct tctttgctag tttcttctat
    22321 cataccactt tttaccaaaa ataaaagtga tcatgttaaa aacgtttgtt ttttaacaaa
    22381 agtaagcgct ttattttaaa gaatgtttgg ataggtttag tctccatatt ccactaaaat
    22441 tctgttatac tagtcttgac tatattgtta aggagacaac tatggaattg aaaaaacgtt
    22501 cagaattccc tgaaaatgaa ctttgggatc taacagcgct ctacaaagac cgccaagatt
    22561 ttttactcgc tattgaaaaa gccttacaag atattgacct gtttaagcgc aattatgaag
    22621 gccgcttaac gtcggtggac gatttcactc aggccttaat agaaattgag catatttata
    22681 ttcaaatgag tcatattggc acttatgcct ttatgcctca aaccactgac ttttcagatg
    22741 aaagttttgc tcaaattgct caggctggcg atgatttcat gactaaagct agcgttgccc
    22801 ttagcttctt tgacaccgcc ttagccaatg ccgaccttga cgtgttagat acattagaaa
    22861 agaaccctta tttcagcgca gctattcgca tggcaaaaat tcaaaaagaa cacctcttaa
    22921 gtcctgatgt ggaaaaggct ttggctaact tacgcgaggt tatcaatgcg ccctatgata
    22981 tttacaccaa aatgcgggct ggtgactttg acatggagga ttttgaggtg gatggtaaaa
    23041 cctataaaaa tagctttgtt tcttacgaaa atttctatca aaatcacgaa aatgctgaga
    23101 ttcgtgacaa agccttccgc tctttttcaa aaggtctccg taaacaccaa aatacagctg
    23161 cagcggctta cttagccaag gttaaatctg aaaaattgtt agcggatatg aaaggctacg
    23221 cttctgtctt tgattacctc ctagctgaac aggaagtgga tcgtagctta ttcgaccgcc
    23281 aaattgacct catcatgact gaatttggac cagttgctca gaaattctta aaacatgtcg
    23341 cccaagtcaa tggtcttgag aaaatgactt ttgctgactg gaagctagat attgacaatg
    23401 atctcaatcc agaggtcagc attgatggtg cctatgacct cgtgatgaaa tctctagccc
    23461 cattagggca agaatacacg aaagaaatcg aacgttatca gacagaacgc tgggttgact
    23521 ttgctgctaa cgccaataag gattctggtg gctatgccgc tgatccctac aaggttcatc
    23581 cgtatgtctt aatgagttgg actggaagaa tgtctgatgt ttataccctt attcatgaaa
    23641 tcggtcactc tggtcaattc attttttcag ataatcacca aagttacttc aacacccata
    23701 tgtcaactta ttacgtcgaa gcgccatcga cctttaatga attgatgctt agtgattact
    23761 tggaacatca atttgatgac cctcgtcaaa aacgttttgc ccttgctcac cgtttaacag
    23821 acacttactt tcataacttc atcactcacc tattggaagc ggctttccaa cgtaaggttt
    23881 acaccctgat tgaagaaggc ggtacctttg gagctgacca attaaatgcg atgatgaaag
    23941 aggtcttaac agacttctgg ggagatgccg ttgacatcga cgatgacgca gctcttacct
    24001 ggatgcgcca agcgcattac tacatgggct tatatagtta tacctattct gctgggcttg
    24061 ttatttcaac agctggttac cttaacctta aaaacaaccc agatggggca aaagaatggc
    24121 ttgacttcct aaaatcaggt ggcagccgta cgcctctcga tactgcaatg ctcattgggg
    24181 cagacattgc caccgaaaag cctcttcgag ataccattca attcttgagt gataccgttg
    24241 accaaattat caattacacc gaggagatgt cacatgccta atctctatga ttttacggtt
    24301 aaggctcaaa atggaaatga cctttcctta gctgcttata aaaaaaaggt tgttttgatt
    24361 gttaacacag ctaccaagtg tggattaacc ccacaatacc aggccctgca agcactatat
    24421 gacacatacc atgacaaggg atttgaggtc ttagattttc cttgcaacca atttttaaat
    24481 caagctccag gagatgctga ggagattaat cacttttgca gtctgaccta tcacactact
    24541 ttccctcgct ttgctaaaat caaagtgaat ggtaaagacg ctgatccttt gttcacctgg
    24601 ttaaaagaag aaaaaagtgg tccccttggg aaacgcatcg aatggaattt cactaaattt
    24661 ctcattgacc aaaatggtca agtcattaaa cgctattcct caaagacaga tcctaaacta
    24721 attgaagaag accttaaagc acttcttggt taatataaaa tagacttggc gtgccaagtc
    24781 tattttgtat taatagttac tatttaaaaa cttcttgata aaattcaatg ctatcaccat
    24841 ctttaacggt aatttgatcg gcacccttat ctgccatctt tccattcacc ttaaaaagcc
    24901 agtatttgtt agctttcgtg tcttgctcaa taccatcaat tgctgtgata aagccatctt
    24961 tttctttgac ctcataatta tctttgagaa tctcaagcac cgtatctcct tttccaaaag
    25021 aaactttttc atcaatggta ttggtatctt ctttgacaac taagcgaacg tgatggtctg
    25081 cttttggaac gcttggagtc tgtgtttgtt ttgttccttg cgagcaagca acaagtagca
    25141 tagcaacaag cgctagaaat cctgttttaa atcttttgtt caccgattaa cctcctaaat
    25201 aacatcatca caactggaaa aaagacgaga gttgataaag catgcgctat attaaagggc
    25261 atccctgcta aaacataact ccaccaaggc atactataca aatacgcaaa acaagtatct
    25321 atcaacaccc cataaaggag gccacataag gcggccaaga aagcctgata accaagctga
    25381 tatttgggga attgctgaga caaagggtac aaggcaaaac gccataataa aagaacaagc
    25441 acaaaacaag taacttgcca aaagacccaa gggccaaatc ccagcaaaaa ggctgataaa
    25501 aacaagcaca acatcataac taatacagcc tcggctaacc caaagtaaag aagataactg
    25561 agtaaaaatg ccgtcacagg ctgcacatta ggcaggctgc taaaaaccat tctcaacaca
    25621 acacataggg ctgacatgat agcaatgcga ctcataaact tagctgacat aaacttcttc
    25681 ctcgtttttt tctctattat gacagattta cagagcgttt accagtttga agctcactta
    25741 tttatcaaat gataataaca tgagaattaa ataacaaaac agtacttgct gactgtcatg
    25801 atatagtcaa agactaaact aatattgtaa agaatatagc taccaactat aagagatagt
    25861 gacataaact tgacatagtt agagaggatc tcatttgtaa cttataaaag ataatacctt
    25921 aaaatgcaag ataatataag aaaacagtta ggatattttg atttttttta gtaattaggg
    25981 tacaataaca gttaatgaga agaaaaataa aacctattgt tgtgctagtc ttctttattt
    26041 tactggcaat ggtattgatt atcggaaaaa gacaagctaa tcatgcgaaa caaaaagaag
    26101 ttgaagacgc taagagtcat attcctattg caaccagtaa tcctagtaaa gcaaaaacta
    26161 gcacttctga aaccgaagac tttattttaa atcctattgt tgatgtttcc ggctggcaac
    26221 tccctgaaga aattgattat gacacattgt cacgtcacat ctctggcgca attgtacgtg
    26281 tctatggagg atcgcaaatt acagctcata acaacgctgc ttttacaact ggaattgaca
    26341 aatcttttaa aacgcatatc aaagaatttc aaaaacgaaa tgttcctgta gctgtttaca
    26401 gttacgcact tggtcgtagt actaaagaga tgaaagaaga ggcccgagct ttctacaaaa
    26461 atgctgctcc ctataatcca acttactact ggattgatgt cgaagaagcc actatgaaag
    26521 atatgaataa aggcgtaaca gccttccgag aagagcttaa aaaacttggt gctgaaaatg
    26581 ttggtctcta tattggaact tattttatgg cagaacaaga tatttcaaca aaaggtttcg
    26641 atgctgtatg gattccgact tatggtagtg attctggcta ttatgaggct gctcctaata
    26701 caaccttaga ttacgattta caccaatata cctcacaagg ttatctcagt ggttttaaca
    26761 atgctttaga tttaaatcaa attgccgtaa caaaagacac taaaaaaacg tttgagaagt
    26821 tatttggcaa ctccaacaat taagaggtta gcatcatggc taatcttttt ttatagttta
    26881 catcttacca agaaaaactc taggtcaata atttaaaacc ccttatcctt gacaaggaga
    26941 ttatctcatc atataatgaa agcactataa tatttcacaa aggagtgctt tggctgagat
    27001 cgcaattgcg aaatcctgag gacctgatct tgttagtaca agcgtaggga ttgtgaccaa
    27061 taatcaatga ttggaaagcg atactctttt tgtgcacaaa aggagttttt tatgtcacca
    27121 aacaccaatg tcaaatacct tatcgaagct gctatttttg ctgcactggc gatggccctt
    27181 tcatttattc cagattttgc tggctggttt agtccttctt atggtgctat tgcactggtt
    27241 attttctctt tgcgccgtgg gttaaagtac ggcatgttgg cgggtctcat ttgggggctg
    27301 cttcactttg tacttggaaa agtttactat ctcagcatgt cccaagtctt tattgaatat
    27361 atcttagcct ttacctctat gggattagcc ggtagtttct ctgatagttt gattaaaact
    27421 ttaagaagac aacaaacctt ttttgccgtt tttcttgcaa taatggctag tttactagcc
    27481 gttacagtac gttacttgtg gcacttcctt gcaggaatca ttttctgggg cagttacgcc
    27541 cctaaaggca tgtccgctgt ctggtattct tttagcgtta atggtacagc tggtgtccta
    27601 acttttttaa taacatgttt agcgttaatg attgctttac caattcatcc tcaattattt
    27661 gatcccaaag actaattttt agtcatttga atcttacatt aaaaaaatgg tataataatg
    27721 ttgttatacc attttttatg tttagataaa ggtaaggact tgatatggct aaaaaaacaa
    27781 aattcaaaaa aaccttagta gaacaaatcc tcgataaggc taatattgct catcaaggac
    27841 tcaagcttaa tgctttagaa ggcgactttc cagatgatct tcagccatca gatatttaca
    27901 agacacttgc tctgactggt gatcaaacag gtcctctaat tggcattatt ccattaacag
    27961 aacacttatc agaaaaacaa ttggcaaaag tatcaggtaa caaaaaagtg tccatggttc
    28021 ctcaaaaaga cttgcaaaag acaacaggct atattcacgg tgccaataat cctgttggga
    28081 ttcgtcaaaa acattcatat cctattttta ttgaccaaac tgcactggaa aaaggtcaaa
    28141 taatcgtttc agctggtgaa gttgggcgtt ctataaagat ttctagccag gctctagctg
    28201 atttcgttgg cgcaagcttt gctgatttaa agaagagaaa atgaacacta ctgccaactt
    28261 tatctggttt cttttttaag tttttcgcct tgattaagct agtctttgtt tagtattatt
    28321 ttgatctcaa ctgctttaac attgatcctg ttaatctttt tcttttgtaa aggcttgata
    28381 tcacgacaaa ttcaggtcga tttgtcttaa atctaatatg ggctttacac tttaatcaga
    28441 acctagctat tatacactta tactacgctt ttttacttta tcttttcact ataggtatta
    28501 tttttgcttg gtgatgccta aactttttct ggttactttt ttatttttag cgctcgcatc
    28561 agttatctct aaaaggaggc ttaaatgaaa ttatattttg taagacatgg gaaaaccctt
    28621 tggaatttag aaggtcgctt tcaaggcgct gggggcgatt cgccattact tgaagaggca
    28681 aaagacgaaa ttcacttact tggcaaagag ttagctaagg tggcttttga tgccgtttat
    28741 actagcgacc ttcaaagagc tatggcaaca gcagctatta tcttagatgc ttttgaccaa
    28801 cagccaaaac tttaccacac cgaccagctt cgggagtgga gattaggtaa attggaaggg
    28861 gctaaaatag caacaatggc agctatatat ccccaacaaa tgctggcttt tcgggagaat
    28921 ctagcccagt tcaaatcaga tcagttcgag gctgaatcaa tttaccaaac gactcaacgt
    28981 gtctgccatc ttatccaatc gtttaaagat aaacattatc aaaacgttct tattgtagga
    29041 cacggcgcta acttaacagc aactattcgc tcattattag gttttgaacc tgctctttta
    29101 ctcgctaaag gtggcttaga taataccagt cttactattc ttgaaactaa ggattattta
    29161 acttatgact gtttaatttg gaatgataaa tcctttctag aggtgtaaaa ccactagaca
    29221 ataataaaaa aagctaacac attgatttct tactgacaag acactcactt tgctagcttt
    29281 ttttattatt atctagtggg ctattatggt ttcatctatc tggcatattt taagcgcaaa
    29341 actttttctt gctgcttggt taaccgaagc aagataacca ccttgtcaat gctcatgtta
    29401 ctttcaagta aacgctctgc tgccatcatc aagccattat tgattcccat ctgtaaaatt
    29461 ggatttttct tgtcattgac atcaaaaata aatttattat caggtaaatc aaagagtact
    29521 ttaacggcac caaagagctg ttcaaagtta tcaatagcca catcataaac aggcttagtg
    29581 cctggtttta gctgacgacc tctatgatta aaccacatgg aatgccaacc accattaaag
    29641 gcacccatga tatcattgtc ataagagtcc ccaacatata gtgtggtttg tgggttcatg
    29701 tcaaattgtt ctgctgccag gttaaaaatt tctttttctg gtttttgaaa gcctgttgct
    29761 tggctcacaa tgacacgctt aggatcaata taatcataaa gccctaattt acgaactttt
    29821 ttcagctgat gttctgtggg cccattggta ataatcccca taggaacatt ctttgatttt
    29881 aaaaagtcaa gggtcatacg catctcatct aacatggtaa tgttctcaag ttcgtgctca
    29941 tagacttctt gaaaatgtac tcccgcagct tcgtcgattt ctcgatagcc aaactcaagc
    30001 agtgtttcct tgcaacgcca gaatcggaaa tattctgtgg tccactcccc tgccattact
    30061 cttgggaatc caacatctga atagtgacga aagcgaatat aagcttgatt cattactgac
    30121 atatcaaaat ctgggaagca cttttccatg gcgattcgat aaggcgcttg ctggtcgtaa
    30181 attgtgtcat ccacatcaaa aacaatagca gtaatcatga gtctcttttc ttttctagtt
    30241 taatatccca atcattatac tattttccat taggcttttc aatttcattt tcaaaaaaat
    30301 gccccgctgc agtcgcattt tccaacgaaa tataggtgta ttctagcccc ttttatgata
    30361 caatggaaag gaaatcgtat tggaggaaaa tatgtctaat caacacatcg aagaattaaa
    30421 tgaccaacag attgtccgtc gtgaaaaaat gacggcgctg gctgaacaag gaattgatcc
    30481 tttcggtaaa cgctttgacc gcacagccaa ttctgctgaa ttaaaagaaa agtatgctga
    30541 taaaacaaaa gaagaattac atgagttaaa cgaaacagct attgttgctg gccgcttgat
    30601 gacaaaacgt ggcaaaggta aggttggttt tgctcatctt caagaccgtg aaggccaaat
    30661 ccaactttat gttcgcaagg actctgttgg cgaagacaac tatgaaatct tcaaaaaagc
    30721 cgatttaggc gactttatcg gcgttgaggg cgaagtcatg cgtacagaca tgggcgagtt
    30781 gtcaatcaag gcaaccaagt tgactcacct ttctaaatca cttcgtcctc ttcctgaaaa
    30841 attccacgga ttaactgata ttgaaaccat ttaccgtaag cgtcaccttg atttaatttc
    30901 aaatcgtgaa agctttgacc gcttcgtgac ccgttccaaa atgatttcag aaatccgtcg
    30961 ttatttagac ggtcttgatt tcctagaagt agaaacgcct gtgcttcata acgaggctgg
    31021 tggtgctgct gctcgtcctt tcgttactca ccataatgct caaaacattg atatggttct
    31081 tcgtatcgca acagaacttc atttgaaacg tttgattgtt ggtggtatgg aacgtgtcta
    31141 tgaaattggt cgtatcttcc gtaacgaggg aatggatgct actcataatc ctgagttcac
    31201 ttctattgag gtttaccaag cctatgcaga ctatcttgat atcatgaacc tgacagaagg
    31261 cattatccaa catgctgcta aagctgttaa aggtgatggc cctattgatt atcaaggaac
    31321 tgaaatccgt atcaatgaac ctttcaaacg tgtccacatg gtagatgcta tcaaggaagt
    31381 gacaggtgtt gatttctggc ctgaaatgac tgtcgaagaa gctattgcac ttgccaaaga
    31441 aaaacaggtc ccactcgaaa aacacttcac aagtgtcgga catatcatca atgctttctt
    31501 cgaagaattc gttgaagaaa ccttggtcca accaaccttt gtgtttggtc atcctgttga
    31561 agtatcgcct ttggctaaga agaatccaga agatacccgc ttcaccgacc gcttcgaact
    31621 tttcatcatg accaaagaat acgccaatgc tttcacagag ttgaatgatc ccattgacca
    31681 attatctcgt tttgaagcac aagcacaagc caaagaactt ggtgatgacg aagcaactgg
    31741 cattgactac gacttcgttg aagctcttga atatggtatg ccgccaaccg gtggactcgg
    31801 aatcgggatt gatcgtcttt gcatgttatt aacaaacaca acaacaatca gagatgtgct
    31861 tttattccca acgatgaaac cttaactttt ttaagatttg tattcatatc ttaaaaccga
    31921 taagtggcaa tataacataa cgttttatta tctctaacca gtcttcatta ccatgaagac
    31981 tggtttatta acagtagctt atagaaagtg acttaacaag atgaccaaag aatatatctc
    32041 ctacattagt gctgctatct tcttgcttta tggcctttta gctgccaaat ggatttttct
    32101 tgggctaggc ttaattttgg tacttgtcgg ccttgctgat cgcctcaatt caaaaaaata
    32161 atccttctat tcgcccctgt ccttttaacc tagcgatgtc atcaccaggt taaaaggaca
    32221 agggcgaacg agggattatg tttcgattaa ggtaacatta cttacgaaaa gcgtctaaga
    32281 tggttttaaa ttcttcatta gtaagagtaa tgcctttccc cattttcgta tggtctggac
    32341 tccacgtgcg gatatcccat ttcgcttctg cgccattaaa gctgacgcgg ttcaattctt
    32401 tcgtccaacc tttatcattt tctgataagg tgagtaaatg ttcctcaatg ttgaatgtaa
    32461 attctgccat tagttttcct cctcatctat actattcgaa attaaagtgg aaaaaatcat
    32521 ctttttttca ctttttttat gactcattct cctattattc tatcaagttt tgagaaaata
    32581 agctattgtg atatccaatt ttcaaaaaaa aaaaaaaaac aggctctctt ttcccaacta
    32641 tggtcgtata ccatttggaa aaagagggcc tctaatattt cttgttacta tgttctaaca
    32701 gttttactgg tgccatcttt ttgatagagg ttgacaaggc cttccttgca agccctaatc
    32761 atatcccctg gcttaacttc tctcggtaaa gagatggtta agagttccat tggatttggg
    32821 gcacggtcaa tcttttggcc atccgcatca tgtaagtcct taacaaccgt ttcaaaatga
    32881 cggaaacctg gtccataaca ttcaatccga tcgccttcca tgatgacatt acgctgacga
    32941 atggtcgctg tcatgctagc tgagtcaaag gcaactactt ctccgacaaa tttatattgt
    33001 ggaattttgc ggcgagcacc aaataattgt tcattttcag ttgggatacc ataataaaaa
    33061 cctgtagcca actcgcgctg ggcaaccttc cacaactcgt caatcaattc ctctttgata
    33121 gcataaaaag cttctgggct ttccatgtaa gcatctacag ccgccttgta acagttggtt
    33181 acggttgaga cgtagtggat agatttcatt cggccttcaa tttttaagct atcaacccca
    33241 ttttcaatca ggtcaggaat atggtcaatc atacacatgt caacagagga catagaatag
    33301 tcttctggaa tttccccttt taaggagcgg cgctctcctc caaatggcat gtcatacaaa
    33361 tcatacttcc agcggcaaga ctgtgagcag ccgcccctgt tggcatcacg gtgactcatg
    33421 tggtttgaca aaacacagcg gcctgaataa gaaatacaca tggctccatg gacaaaggct
    33481 tcaatttcca catctgtccg cttacggatt tctgctaact cggccatatt aacctcacga
    33541 gctaaaacaa ctcgggtcaa acccatggct ttccaaaatt caaaggtctc gtaattggta
    33601 gatgaagctt gcgttgacaa atgaatttcc aaacctgggg cttctgttga acaaataata
    33661 atcaaggctg gatctgaaac aatgaccgca tcaagcccca tatcacgcag ttgacgaaac
    33721 cactcgcccg caccaatttc gttcccttcg tgggtaacca tgttggcagc aacatagacc
    33781 ttagctccac gcgcatgtgc ataatcaatg ccttcttgca attcttccat agagaagtta
    33841 ccagcgcggc ttcttagacc ataggcctgt cctccaacaa aaacagcatc tgcgccatag
    33901 tcaatcgcaa cttttaattt ttcaagtgtt ccagcaggtg ataagacctc gggacgtttt
    33961 ttcatatgtg acatgtgata ttctttctat tttcaagata ggtagattac ctgtgtaatg
    34021 gtgtcataac cctgtcttgc cctgaaatag taaactcact caagacaagg attacttatt
    34081 taacggtttt tggatcgaac tcataaaagc cagtatctaa tccacggcca gctggatgat
    34141 gggcatgcac tgcttgatca agcttttcag cttcttcttg ggtaaattgc ccagcttcta
    34201 ataaggtttt agcctgaata aacaatttag taatagcgac aaaatcatca cctggacagt
    34261 aaatgccatc tagcttccaa tgagttaagc catgcgcata cagctcaccc aatttagaca
    34321 tcatgtcgat atcgttatta atgaaaatat gagtgccatg attgtcttca taaatggagt
    34381 aatggctgct agcatctcct ggttctgcca agaaaagtcc tcgttcacga ctaacctcat
    34441 catcaatctt tgtaaaatgg taataatttt ctagcaaggg acgtttcgag tgatgaatca
    34501 ccgaggcgcc gtaaactagc acctctgctg gaaattccaa attttcagct aaggtgaata
    34561 actctgctga tgggatttcc cttgctaaaa cactttcaac agcaccatgt tgcccccaga
    34621 aatttacctg acggctagaa gtcacaaaaa cagaggtatc ataaatcagt ttaaagttat
    34681 agccatccct tttattgaca taaaacacac ctgcatcacc aaccacaaga taatcaacag
    34741 caatctctat cattaagtct aaaaagggtt tgatctgatc catcatatct tgatgcatca
    34801 aagcattgca agctactgtc aactctttgc ctgcatcatg cactagtttg gcaatttgtc
    34861 tcaattcatc ataagaaaaa ttatggggaa ggcgtaaccc gtaattggct tcaccaacat
    34921 aaatacgatc aacaccagca gctaataaag ctttgacttg ctcaatgctt tcagctgtgg
    34981 ctgtaataat tattttttcc atataagctt tattatacca aaaattttaa gaccttaagc
    35041 atatttcttg caaaagccca aacgttaagc tagtcccctt actgttatag catgataagt
    35101 cacttgtcat tagtaaatct tttcaaatct tttaaaaaaa gatgacaaga agagactttt
    35161 atgatagaat tacttagtat gttaaggaaa ggagaggact atgcctgcac cgttaaagaa
    35221 gcataattcg gttgaacagg atgattctta ccaagaatct caaagtaaat ttcgagattt
    35281 tcaagagttc aatggccaaa gtgcaaagct aaaagaatta gtcttctttg ctcgtatcgc
    35341 tagtttcagc cttattaccg ttttatttgc atttgtcctg ctcgtaatga atttagcgcc
    35401 aatgtttgct ttcttatttg caagcatcat cagcttaggc attacttcct tattcgccaa
    35461 ggttatcaaa tcattactga tgtaacttcc agagtccgtt ttacgtgact cttttttttg
    35521 tgtcaaaaac aaaaaaagat tgaagccaca atccccccct tcttcagtta gcctaaaact
    35581 aaccatcggg actagtgatc agatccttca atcttttctt cttctatgtt ttaggtcgtt
    35641 ttagttagtg tttatctagt gtatcactaa caggctcatc ttgttcagta tagtcaatga
    35701 tgatgtcttc ttcatttaaa tcaatgataa catcatcttg cttttctttc gcttcaactt
    35761 cggcaagggt ttcttttgtt ttttgcacga aagctgttgt tttatctttg agcaagtcta
    35821 acatgtcctc tttagttaag tcaccagaag ccaacttatc tttcacatca tagaaagtgt
    35881 cacgtgctaa atggctgtat tcactacctt tttctttagc aaattggtgg taatcatctg
    35941 gactttcttt gtaggcttga taagcttttt cagcacgatt tttcaaagcc ttgccttttt
    36001 ctgttgataa gaagtaagct gccgctactc ctgaaacagc tccaatcact aagtttttga
    36061 aacttttatt catgcttttc tccttttttt ccaaatactt ttgaggctac tttaccaact
    36121 aatgctaatt ttccagcttt tgaaacatta cctgtagcat tagttgcttt ttgcccaaaa
    36181 tgccttgctt gactatttaa atctgaaaga ctttctgaca aatcagcaat agcaacaaac
    36241 agtggatcga tggttgctac tttaccatta acatcttcaa caaggatatt agcttttgct
    36301 aaaatatcat tagtttgatg aagtgtcaca ttaacatcac ttgtcaatac agaaatcgtt
    36361 tttttggctt catcaattgt ttctgagact ttctttaata caataatcaa gaaaatcacc
    36421 aaagcaacaa atgcgagtgc aataatcatc agtgagattc caactaagtc cataagtttc
    36481 tcctttttct atccttattc ttgataataa gaaattcctt tttgacggcg tctttttatg
    36541 acaaagatta agccaataat aattaataag gcagacacat attgggagat gcgaatacct
    36601 aaaaacataa gactatctgt tcgcatacct tcaatgacta gtcttccact accataccaa
    36661 attaagtaaa atgcaaagat ttctccatct aatagacttt ttggcttacg acgccacatc
    36721 ataataatga caaatcccaa aagattccaa agtgattcat agagaaaggt cggaatgcgg
    36781 taacttccct ctataaacat ttgcttttga ataaagcttg gtaagtaatt taactggctc
    36841 acagctttac cataggcttc ttggttgata aaatttcccc agcgaccgat agcttgagcg
    36901 accatgacac ttggtgcggc aatatctaaa aaatgaatgg gattgagcac cttgttataa
    36961 cagtaagcca aaagtacaag agctcctgta atgagaccgc cataaatagc aataccgcca
    37021 ttccaaatgg ctatgatttc atctaaatgc ttgacataat aagaccattc aaaaataaca
    37081 tagtaaattc ttgcaccaac aatagcaagt ggaaaagcaa tcagaataaa atcgaaaata
    37141 gcgtcagatg atattccttt tttgggtgct tcttttgatg ctaagtaaac cgccaaaaca
    37201 agtcctgaca agatacatag cgcataccag tggatggcta gtggaccaca ttttaaggca
    37261 atgggattga tcattgactc acctcatttt tggtgattag ttgagttaaa cgatcttcaa
    37321 aagtttttgt agcatcaaag cccatttctt tagcccgatg attcatcgca gcggcttcaa
    37381 taaccactga aacatttcga cctgttttta ccggaatacg aatacgaggg attctcactc
    37441 ctgagaaagt tatctcctca ttaccgtttc cgagacgatc aaaaacttta ccagcctcaa
    37501 agttttcaag gtaaatggcc aattgaactt gtgatgagtc cttcacagca ctagctccat
    37561 aaagcgacat cacatcaata atacctaccc cacgaatttc aagcaagtgg cgcaagatct
    37621 ctgctggctc tccccaaagg gtttcttcat ctttggcata gacatccaca cggtcatctg
    37681 caaccaaacg atgccctcgc ttaactaatt ctaaaccagt ctcactttta ccaattccag
    37741 aatccccttg gataagaacc cccataccat aaatatccat taaaacgccg tgaacactag
    37801 tacgttcagc taaagaagcg tcaagaaaat aagacatttc tccagctaag cggctagttg
    37861 aaacacggct acttaaaagt gaaatacctt cttcttttgc agcttgaacc atttcttttg
    37921 gaatggctaa atttctagag acaaccactg caggagtgtc ctttttgaac atctctttta
    37981 aaacagaata acggttgtga gaagtcatct gtgtaagata agaccactct ttcattccaa
    38041 atagctgcaa cctttctgga gcataataat caaaataacc agtcatttca agacctggac
    38101 gtgaaatatc tgatgttgtt atctcttttg acaacaagtt atctgtagca taaaccacat
    38161 caagtttaac tttttgcact agcatcttta cagtaacggt cataatttct cctttttcaa
    38221 tattataaca caaatcgacc gttagaccct tgcaaatact cattttttca ctactaaatg
    38281 agaacaacga caccaaaaat ggtcttagct ctattaaaaa ctaggtttta tcatttagag
    38341 atatgagtca aaaataagac aatggcaaac aaatacttta ctagtatcat tgagcaatta
    38401 gtgcttatta tggtgtttaa aaaggtatca tcttactaag tcaaaaagca aaaagcctca
    38461 tcatagctga taagacccct cgcttactta catatcaaaa cattttatta tcatcagtta
    38521 atcacatggt tgctcttacg tgatgaatta tcattttaac tgattgttta ccagaaccag
    38581 ccatcttctt cttcatttaa gacatcagcg tctttacgac gacgaggacc tcggccataa
    38641 cgttcttcaa gcaagtcttc cttgtaaggt aagaagatgg ccaacaaaat ataaagcaaa
    38701 actccaaaac cagttccgta aataagaaga gccgctaaga cacgcgctag agcaagatcc
    38761 caaccatact tatctgctaa accagcaata acacctgcaa ccaagcgatt ttttcgctgt
    38821 ttatagaatt tagtttccac gtttctttac ctcctcattc tatgatacta gtttacattg
    38881 tttttcgcaa aataaaacca gtcttacgac tgattttttt ccataagttt cccgtggcaa
    38941 tttccacaaa catatttagc cagattgata cgcctttttc gttgataaac ctgcccacag
    39001 gtttggcaac tataatgatg attagtcttt gatttagagc tagttgggac ataacgaagc
    39061 ccgtttactt gagctagcag atctttgaaa tccctatctt tatgatggta ccctcttcct
    39121 tcaaaataaa gatggtaatg gcagagctca tggcgaacaa ttttccgaaa aataagctca
    39181 ccgtgctcct caagcatcct agggttaaaa tccaaatgac catcttttgg aaaaaagcga
    39241 ccgccagttg tttttaggcg cttgttccaa taagccttgt ggtgaaatgg tttgccaaaa
    39301 tcagccaggg aaacttcttg gacataatta gtgagtgtca cgcggtggca ataggctgag
    39361 attgaccttg tggcggtcca agtcaatctt agagacccaa acagttacca agtcacccac
    39421 tgaaacaact tgactagggt ggttgacaaa ggttttgctc atttcagaaa tgtgaataag
    39481 cccatcttca tgaacaccaa tgtctacaaa agcaccaaag tcaacgacgt ttctcacagt
    39541 tccttcaagc ttctggccaa tttccaaatc tttcaaatca aggatatctt gacgtaagat
    39601 tggtgcttca aagtcatcac gaaggtcacg acctggttta aggagatcag caatgatatc
    39661 tttaagggtt tcctgcccaa tagccaatgt ttctgccatt tggggaactt gaactgctgc
    39721 taaagttgcc ttggcagcgt cgtccaagtc ctgaatacca agtaccttaa agagttcttt
    39781 gactgctggg taggactcgg ggtgaactcc tgtgttatct aaaatatttt tggctcctgg
    39841 aattctgaga aaacctgctg cttgctcaaa cgccttagct cctaaacgcg gcactttttt
    39901 gatgtcagcg cgtgatgtca atgctccatt ttcttcgcgg tatttgacaa tattttctga
    39961 aatcgttttg ttcaaacctg acacatgtgc taataaagat ggactagccg tgttcacatt
    40021 aacaccgact tggttaacca cagtatccac aacaaaacca agattctcac tcaacttttt
    40081 ctgactcaca tcgtgctggt actgaccgac gccaatagat ttaggatcaa ttttaaccaa
    40141 ttctgctagt gggtcttgca agcgacgagc aatagaaata gcagagcgct tctccactgt
    40201 gaggtctgga aactcatgac gggctaactc tgacgctgaa taaaccgaag caccgctttc
    40261 gttaacgatg acataggacg tatttgggaa atctttcaaa acgtctgcta caaaggcttc
    40321 actttcccga ctggctgttc cgtttccaat ggcaataata tcaatctggt aagtctcaat
    40381 gagctgagtc agtgtttctt tagctgcttg aatcttcgtt tggctagctg gtgctacggg
    40441 ataaataacc tgagtagtca acaattttcc agtctgatca acgatagcca attttgcacc
    40501 tgttcgaaag gcagggtcaa acccaagaac cattttccct tttaaaggag acactaacaa
    40561 aagatgacgg agattttcag aaaagaggtg gatagcccca tcttctgcgg catcactgag
    40621 ttctgaacga acccgtcttt ccatagctgg aacaattttc tttttgatgg tctgattgat
    40681 gacctcttca atataagggt tggtttcttt gaaccgcaca ctgaaaaagc gttgcatttt
    40741 ctccaggtta tgctcaaaag acacttttaa gatgcctaac ttttcgccac ggttaagggc
    40801 taaagtacga tatccttgca tgttagacac ttggtcagaa aaatcgtaat agatttggaa
    40861 aacctttttc tcatccaact gctcatcttt aagcgttgat actaaacggc tgtactgcca
    40921 aatctcattg taggtccaag agcgtaattt agcatcttct gacatggcct ccacaagaat
    40981 gtccacagct cctgctagag cttcttgtgg gctggcaaat ccttctgtga caaagggttc
    41041 tgctgctgtt tcaaggtttt gcgcattttg taaaatcaaa cgtgctaatg ggaacaagcc
    41101 tgcttcacgc gcaatcgtcg cttttgtacg gcgtttttcc ttataaggca ggtacaactc
    41161 ttccaaatca gcgagttttt cggttgcttc aatactcgtt cgtaactgat ctgtcagttt
    41221 tccctgttcc tcaattttag cgagaatggt tgctttgcgc tcattcaggg tagtgagaga
    41281 cttatccata tcaataatgg acttaatcac cacttcatct agatttcctg ttacttcttt
    41341 acggtaacgt gcaataaagg gaatggtatt gccttgtgct gttagggcaa gcacctgttc
    41401 aatttgatgg agactaactg acaaagcctc tgcaatgttg tgattattgt tattttccat
    41461 aatattgcta ttataacaca aaccatgtta atgcaaaaaa tgatagcgaa acattgtcct
    41521 aatataaagt tgcgtaaaga atcaaaaaaa gctactttta gtaaataact gacgaaaccc
    41581 taaataacaa tacctaattt taaaaaagaa agaagtaaac agcacttccg ctttggcatt
    41641 gttctcaaat gatagtataa tagacttatc ttgaatgaaa gaaggtcata gatatggcaa
    41701 ttacccataa aaaaaatgat gaacttgaaa aaatgctagc aggctttgca tcaatcccta
    41761 gttttgataa gcctttggaa gtcaatactg atggtaaact agcgaccaaa gaaaaaacga
    41821 cagaacaaac aaaaccaagc aaaccatagc tagtttgtct cattctctta acatatcgtc
    41881 cattcgttta aactgagttg agagattttt agcgatcagg ctaacaaaac gtatcagtcg
    41941 aggctgaaca ttcgtgtttc agcctcctct tttatcatct agaaaggata tggtgaatgt
    42001 cactcttttc caaccttcct cccagtgtct tgcaatggtt tgcgattttt atgtccatta
    42061 tcattgaagc tcttcctttt gtgctcctag gaactatttt atctggttgt attgaagtct
    42121 ttgtaacgcc agaattggtt caaaaatacc tacctaagca aaaatgcttg cgcattctgt
    42181 ttggaacctt tgttggattt gtttttcctt cttgtgaatg cgggattatt cccatcatca
    42241 accgcttttt ggaaaaaaag gtgcctagtt atacggccgt cccttttctg gcaacagcgc
    42301 ctattattaa tcctattgtt ttgttcgcta cttattctgc ttttgggaat tctttgcgct
    42361 ttctgatact tcgcttggtc ggtgctgcac tagtagccat tacacttggg gtaatgttag
    42421 cttttattgt agatgataac attctcaagg ataatgctca acccgtgcat tttcatgatt
    42481 attctcatga aagcttgcca aaacgcattt atctagcact ggtacatgct attgatgaat
    42541 tcttcgacac cggtaggtat ctcgtatttg gaaccttgat tgcctctgcc atgcaaatat
    42601 atgtcccaac tcgtgtgttg acaacgattg gacacaatcc cttgacagcc attttgatca
    42661 tgatgctgat ggctttcatt ctatcattat gtagtgaagc cgatgccttt atcggagctt
    42721 ccttattatc cacctttgga gtggctccag ttcttgcctt tcttttaatt ggtcctatgg
    42781 tggatattaa aaaccttatg atgatggtaa agacttttaa aggacgcttc attgtccaat
    42841 ttatcggcgt atccgttctt atgattgccg tttattgttt acttgtgggg gtgctctaat
    42901 gattcgcttt ttaattttag ctggctattt tgaactaacc atgtatctcc agctgtctgg
    42961 caaactggat cagtacatca atgttcggta ttcttacctt gcttatattt ccatggtttt
    43021 atctttcatt ttagctcttg ttcaactcta tacctggatg aaaaacatta aggtacatag
    43081 ccatttaacc ggaaaaatcg caaggctgac cagtcctttt atcttggtct ttcctgtttt
    43141 gattggtctc cttgtgccga ccgtaacctt agattccacc acggtttctg ccaagggtta
    43201 cacttttcct ttggctgcgg gagcctctaa aacgggagtc agtgatgacg ggaccaccat
    43261 tcaatacctc aaacctgata ccagccttta ctttacaaag tctgcctacc aaaaggagat
    43321 gcgtcaagaa ctccataaat ataaaggcaa gaaaccggtc accattacca ctgaaaatta
    43381 tatggaagtg atggaattga tctatctcta cccagatgag tttcttgatc gtgacatcca
    43441 atacactggc tttgtttata acgaaccggg ccatgacaac taccaattcc ttttccgctt
    43501 cggcattatt cactgtattg ccgactcagg agtgtacgga cttttgacca caggtaatca
    43561 aaccagctac cctaacaaca catggctaac agttaaaggt cgccttcata tggaatatga
    43621 taaaaactta gaacaacatc tacctgtcct tcaacttact gaggttcatc aaaccaaaga
    43681 gcctaataat ccttatgttt accgcgtttt ttaaagacta ataaaaaagc acttgttttt
    43741 tatcaaaaca aatgcttttt gagttttatc agttagcata ggaacagagt caccatctta
    43801 aacaagacct atttaggaaa aattatcctc aaggtaacgt ccattggttt gaatgacttg
    43861 tcggtaccaa tcaaacgatt ttttcttata gcgtgtgagt tgaccagtac catcatcatt
    43921 tcggtcaaca taaataaaac cgtaacgctt acttaattgg gctgttgaca tagagacaca
    43981 atcaatacag ccccaagcag tgtaacccat gacctcaacg ccatcttcaa tcgctttggc
    44041 aacttgcacg agatgctgac tcatgtagtc aatgcgataa tcatcgtgaa ccgtcatgct
    44101 gccatctgcg gtttgcacca gctgatcctt tgcacctaag ccattttcaa caataaacaa
    44161 aggaatctgg tagcggtcat agtactgatt caagaccaat cgcagaccaa ttgggtctat
    44221 ctgccaaccc cactccgaag attctaaata tggattggac aaaccaccta gaatatttcc
    44281 ctgaccagat gtgtaagctt caggattgtg ggcttgtgtg acactcatgt aatagctaaa
    44341 ggatagaaaa tcaaccgtat gttccgctaa taattccttg tctccgtctt caaaaacaat
    44401 ctcaatccca ttttctttaa aatacgactt aatatatgaa ggatacttgc ctctcacatg
    44461 aatatctgaa aataagagat taagattttc aaatgcatgc gcagcaagta tatcacgagg
    44521 atcagaggtc attgggtaag caggcatcgc caaaatcata cacccaactt taacatcagg
    44581 attgatttca tgcgctagct ttgtgacact agcagatgct accaattcat gatgaatagc
    44641 ttggtataaa tcttgcaggc tcagcttttc tttttctgtc atgatgccac cactggtaaa
    44701 gggcatgtgt aaaatcgagt tgacttcgtt gaaggtcaac cagtatttga ctttgtcctt
    44761 atagcgttcc atgactgtct gagcaaagcg ctcaaaaaag ccaatcagac gtctatctgt
    44821 ccaaccatta taggctttgg ccaaatgcag aggtgtctca taatgcgaca aggtcactaa
    44881 gggttcgata ccatagttca acaattcatc gaataagtca tcatagaatt gcaaacctgc
    44941 ttcattgggc tggtcatcgt caccatttgg aaaaatacga gaccaagcaa tagatgttcg
    45001 aaagacttta aagtccatct cggcaaaaag agcaatgtct tctttgtagc ggtggtaaaa
    45061 atcaatcgcc tctaaagtta aattatcgct ggtaggacta tctgtccatt ctcctagtcc
    45121 accatttggt agaacatctt gtacggacaa accttttgtg tctgcctcaa atgctccttc
    45181 cacttggttg gcagctactg cgccgcccca taagaaatct tttggaaaaa ttcccattct
    45241 ccctctcctt ttaggttata aaatgactaa caaagcgtcc tctttagtca ccattttttt
    45301 gtcatcttga ataataatct ccaactgctc agccgaattg gtcacgacca caggactgat
    45361 gagtgaatag ccttttgaag taatgaaatc aatatccatt tttagaagtg cctggcctcg
    45421 tttcacgaca tcgccaacag acactaactg ctcgaaacct tgccctttta actcaaccgt
    45481 atcaataccg acatgaatca agacttccac accacgtgtg ttttttaagg caacagcatg
    45541 cttggttgga aagaaagtga tgacttcacc atcaaagggg gcatagacca caccttcgct
    45601 tggtaatatg gcaaatcctt ctcctaataa cttggaagaa aaggtttcat caggaacttc
    45661 tgatagaaaa agcatctcac ctgtcattgg agcatacaac gtttgtttgg ttgacaagcc
    45721 gctcttaact tgtgacatat ctgctgcaac actcacttgc tttggggact cttcatcaat
    45781 tcccatgaac caagtcaaac tgaaggctaa cacaatcgtc gcagctccgg caatcaaagc
    45841 atttataaaa ttagctccgc ctgatgggtt gatgtactgt ggtaaaccaa taatagaagg
    45901 tacgacaaag gtataagaag caatattgac aaaaccaata aaggctccaa ccagaccacc
    45961 tgaaatcatg gctgcataga gtggcttttt aaatttcagt gtgaccccat aaagtgccgg
    46021 ttccgtgata cctgccagca aagctgaaat ccccgcggca agggcaactt gacgtgtttg
    46081 tttctgcttt gttttaaagg caacagccaa agatgcggct ccttgagcca aattagaagc
    46141 caacattgct ggtaaaatca agacatctgg tgttgcgacc gaagcggcca aaaagattgg
    46201 tgcaaaagcc cagtgcatcc ccgtcatgac aacaagcggc ataatcgctc ccaaaatggc
    46261 aagtgctagc cacggagcca catgataaat agcgagcatg gtattagata acccttgacc
    46321 aataatcaca ccaagaggac ctacaaccac taaagccaaa aagccagaaa tcaaaatgat
    46381 taaggttggc tgtaagaagc ttttcatcac gcttggcacc aagcgattga cccatttttc
    46441 aatgtattgc atcaagtaaa ccatcatcaa aatgggaata acagatgagc catagctagc
    46501 aggagtgact ggtgctccaa ataaagtcaa tggtttacct tctgctacca ttgcaacaaa
    46561 ggcaggatgc aataaaatcc ctgcgatcgt cgctgctaaa acaggtgtca ccttaaagcg
    46621 cgccgctgct gaataggcaa tcatgacagg cataaaataa taagccgcat cgccaaagaa
    46681 gttaataaag gcaacggttt gagagtccgc actcgctaag ccaagcatag gaagcaagat
    46741 tcccaccact ttgagcattc caccaccaag aagggctgga atcagcggtg taatcacgcc
    46801 agcaatggtt tcaatcaagc gctcaaggat acttcctttc gcttttgaag aatgatcttc
    46861 atcttgattg tcaaaatgcc ctaggcttaa aaaggcttgg taataattat tgacatcatt
    46921 gcccaagata atttggtact ggccgttttt gcgcatcacg ccaatcacat ttgaaatggc
    46981 tttgacttgc tgatctttaa ctttctcatc atttttcagt actaagcgta gacgtgttac
    47041 acagtgtgtc acacgttgaa tatttgtttt gccaccaacc gccgctaaaa tagccttggc
    47101 agtttcttga tacgtcatat taagtctcct ttaaatgttt cactaaacgt ttaacatgaa
    47161 ccgtcaaata caaaagttcg cttgaattaa ggtggtagtt gtaccgcttt tttatctcct
    47221 cactaatatc taagacgcac cggtattctt tagggtattt cgttttcacc actaaaagca
    47281 gttccgcatc atcatcatcg taagacatct ctgataacac acgttgagca aataatttaa
    47341 tatgggtcat aaagcggtaa taatcgatgg atgtgtcatc caattctgtt ttgaagtctg
    47401 ttttgatttt ttgctcaata taagaaacaa tctccgtcag gcgatgtggc tccttaaaag
    47461 gagtgtccaa actagcattg acaaaatgca tggcaataaa agctgcttca tcaatagcta
    47521 gagtgactcc tagattacgc tcaattagct ctaaggcttt tacccctaga ctatattcat
    47581 caggataata gcgttgaatt tcccaccgta gtgggttatg aatcaacatt ccttgctcat
    47641 gcctttcaat agcagagtga atatgatccg ttaaattaat atagagaatt tcatctaagg
    47701 ttttgcctaa tttaatttta cctagattga taattctctc tgagcaagca acaacttctt
    47761 gtggcaccgt aataaataac tctgtgaagc gattcatatt gtcactattt tttaaaacaa
    47821 atgatgtttc gatagcatta agctcaatac tatctccaac tttttttcca aaggcaatcc
    47881 cttttcccat caataaaata tctaatcctt gatggttggt cgaaatcgcc gcattatggt
    47941 ttaaaacgcg tttaatcaac ataaaagtcc tagtccttta gtatcacaat aaaaaatgac
    48001 ctatccaaaa gctcgctaaa aagcactttt cgaataggtc cctgctcaca aaactgtgat
    48061 aacatccttt aatattctga ttgttatgtc tctagcctgc ttacttgaag taatcacatc
    48121 tgagaacgct ttaatgttat catctcttta agattctgtc aagcgttttc aaaaaattct
    48181 ctgaaagagc aactattgtt attcgtatct attgatttag caaaaaagag tttctgacaa
    48241 ttttaagatt atatgataca ataacaatcg attagataaa ggagttatca tgtcacatca
    48301 tcaacaaacc gtttcaaaac aaacaattat ggcgattatc gccatagcac tcattggttt
    48361 ttcaggaatt ttgtctgaaa ccagtatgaa tgtcaccttc ccgacactga tgtcagtcta
    48421 tcagttaccc ttaaatagct tgcaatggat gacgaccatt tatttactag cagtggcgat
    48481 tatgatgacc acttcggcta cactgaaaaa aaatgtgcgg gaaagacccc tcttttttat
    48541 ggccacaggt ctttttacat ttggcaccat cttggccgtt ctgacccagt cctttgcgat
    48601 catgttgcta gcccgcattt ttcaaggcat tggtactggt ctggtaatgc ctcagatgtt
    48661 taatattatt ttagagcgtg tcccaatgca taaggtaggt ctatttatgg gatttgctgg
    48721 tcttattatt agcttagcac ctgcttttgg gcctacttat gggggcttta tgattagcca
    48781 ttttagttgg caatggattt ttatctgtat actccctgta ccactgattg caggtattct
    48841 agcttattat tacctcgaag attctccagt aagcgaaaaa gtaccctttg actggttggc
    48901 atttattgca ctatcgatta gcttaacttc tgccttatta gctattacta gtctagaaaa
    48961 cggaagtgtt aatttgtatt acttagggct ttttattctc agctttgtcc tcttcctcta
    49021 caagaatctc acagctaagc aaccctttct tgatattcgc attctcaaaa ttccctctct
    49081 aacctttggc ctgattccct tttttgtctt ccagctgatt aatttaggca taaattttct
    49141 aacgccaaac tttattgtca tggaaaagat tgctaatagt tctcaagctg gtatggtgtt
    49201 actacctggt accttactcg gagctctact agcacctgct tttggtaaac tttatgatca
    49261 aaaaggagca aaactttcgc tttatttagg aaatgcctta tttagtttat cattgattat
    49321 tatgacactt caaacaagac attttatgct tttaccattc actcttttat atattttatt
    49381 cacgtttggg cgtaacatgg gctttaataa tagcttagcc acggctattc gagaattgcc
    49441 tgccgagaaa aatgccgatg ccacggccat ttttcagatg atgcagcaat ttgctggcgc
    49501 tctaggaact gctatggcat cactgatagc aaatagtcaa ccagaattca caagcggtgt
    49561 ccagtctgtc taccttctct ttactatttt tgctctactt gattttatct ttttctttgc
    49621 tatgttttac catttaggga aaaaaggact tgcctaagct gacttactta aaaaccagcc
    49681 tcgttctgtt gaacaagact ggtttttttc ttataaaata ccttctaaaa gacctttcat
    49741 aaagtcttct gagtggaatt ctccaatatc atccactttt tcaccaaatc cgatgaactt
    49801 gactggaatg tctaattctt gacggatggc taggactaca ccacctttgg ccgtgccatc
    49861 aattttcgtt aaaatcaaac ctgttaatgg tgttattttt gaaaattcct tggcttggct
    49921 aagcgcattt tgacctgttg acgcatctaa ggccaaaagc gtttcatgag gggcatctgg
    49981 tagcacgcgc ttaatgatac gacccatttt ttccagctct gccatgagat tttctttgtt
    50041 ttgcaggcga ccagccgtgt caatcaggag aatatctaca ccttttgcca cagctttttc
    50101 catcccgtca aaaacaacag acgctggatc agccttttca gggccagtaa tgactggcac
    50161 atctactcgg cgtccccatt cgactagctg agccacagcc cctgctcgga aggtatctgc
    50221 tgccaccaac atgacttttt taccttcttg tttgtaacgg taagccagct tcccgattga
    50281 agtcgtttta ccaaccccgt taacacctac aaaaagcata accgtcaagc catcttgata
    50341 attaatggct tcattataac gcccgtcttt ttcatagata tctaccaatt tttccacaat
    50401 cacacgtttc aaggcatctg gttttttcgc attttccagt ttggcttcat aacgcaactc
    50461 ctctgtcaaa gttgtcgcca catgtacacc gacatctgat aagatcagca tttcttccaa
    50521 gtcttcaaag aattcttcat ccactcgcct gaaattggca aagaaactgt tcagacgagc
    50581 tgagaagcca gtccgcgttt ttttcaagct acgcttgtat ttttcttgat ctgtctcagg
    50641 aatggtatcg tgggttgcct tgttttgaga agcttctgct gaggtagagg cctgattttc
    50701 ttgtggaact tcttctggca tcactggaac cgttgccgct gctttttctt gaaggctttt
    50761 ttcaagagca gccttacgac gatagtagtc ttccataacc tgttggctac tggattgctc
    50821 ttttgaagtg tctactgctt cttcattcgc cgaatcattt gaggcatcag cttgagcttg
    50881 ttttgttgga aactgttcca caactggctg gtcaacaagg tcttttggtt ctgatgccgt
    50941 agattgacta agttcactta ccttaaggtc tgttttgtcg gcagcaaact cattggtatc
    51001 ttctgatgga tgattactat tgtcacctgt ttcttcctca acctgtgcgt ttactgaagc
    51061 tgcttcggga ccattttcca ccgaacctgc gccctctgaa gaaacgtcct cttgttgaac
    51121 cacctcaatc ttgttttgct cattggcttt ttcagaaagc tcctctttct gagttgtttc
    51181 tgtcaaaaga ttttcttcta acttttcctc agctaccttc ggggtttctt ttttcccaaa
    51241 taaacgatca aataatccca ttaactctcc tcttctaaaa taaatgtctt cacagcttct
    51301 gcgactccag actcatcatt ggttaaggtg gtcacggcat cggcaacggc tttagctcct
    51361 gaaacaccat tagccatagc tactccaagt cctgcccatt ctaacatcgc aaagtcgtta
    51421 gcttcgtctc ccatagccat gacatgtcta gcgtcaaggc caagatgctg gcaaagcaag
    51481 tttaatccaa aagctttatc aatgcctttt ggcatgattt caaaaataat atctcgtgat
    51541 ttaaaggctt caaaatcctc aaataagctt ggggatagct ttataatctg ctgatccaaa
    51601 aagtctggat cggtgactgt cacaattttg ttataggtaa tgtcttttgg tagttccgct
    51661 acagatgtca cctcaataaa ggtcaacagt gggttagcta gatggtactg cgaatgccta
    51721 ccgtccttac ttggaatgct atacacatcc ccaccactga taatatctgt tggtaagcca
    51781 accgcctcta aagcttgctg aatctggcaa acctggtcaa aactgagaga gctcttatcc
    51841 aaaacctccc ctgtatttcg ctgaaccaaa cccccattaa aggtaatact gtagtcatcg
    51901 tggtcaagca agtccaactc ttctagcaag ttaccgatgg cctttagagg gcgacctgtg
    51961 gtaataacca ccttaacccc tttttctcta gccgctgcta aggctttttt attggcatct
    52021 gtgacaattt tttcagtgtt gtagagggta ccgtctaaat caagcgccaa aattctaata
    52081 ttcatgagtt acactagtcc ttttaaatac gttagtactg ctccgtcatt acagtggcca
    52141 atgacttggt ctgaaatcac tttgatttca ggtcttgcat tttccgtagc aatagcccta
    52201 ccagcaaact ccaacatctg gtaatcatta aaattatccc caaaagcaat cgtttctgct
    52261 ttcttaatgc ccaaggcttg acaaagatgc tccataccaa agcccttatt cacctctttt
    52321 aagataatat caatggaatc aaagccggtg gtcactgcag tggcataagg gagggcttga
    52381 ttcaaccaat cgcttccttc taagacggta tggcctgtga agttggtaga aactttaaaa
    52441 atggcgtcat tctccatatc ttcaaaacca ttaataacct taacattagc atagtaatgt
    52501 tttgtttttt gaatgtactc ttctgatgcg ccttttaaaa tataggctgc cttttgccca
    52561 gaaaaaacca tacctgtttc tacatagtga ggattggcta aaaccttttt tgcaacttcg
    52621 gtatactgct ctttggtcat catatctgca aatagaatct caccatggta ttgaaccacg
    52681 gaaccatttt ctgcaataac agcaatctga tccaaaaagg gctcaaagag ctgatcaatg
    52741 gctaagagcg agcggccact cgaaaccgca aataaaatgc ctttttcggc taactttggc
    52801 aagagcgcag ctagttgctc ttgattataa gtaccatctt cagctaggaa ggtaccatcc
    52861 atatctgttg ctattagttt aatcatacaa tgtctccttt ttttactcta ggggggtgca
    52921 aacgccattt agggtgtgca ctttttaacg atttaaataa gccccatcat ttctgcattc
    52981 tgttcaattt ttcttattat atcataaaaa gcagctttca ttccattcgt aaaaaacagc
    53041 cagattatat ttcgataacc tgaccgtttt tgaagtgaac tgctactttt ttatttttat
    53101 aaatgaccac tggatgtata ctataaaagt agacaaaact ttgtgtgtta caatctgttt
    53161 taaaagacac aaaaacgaaa ggacaaccta tgtcaacagt taaacgctac gatgaagaat
    53221 tcaaacaatc tcttgtcaac ctttatcaaa ctggaaaaac tcagtctgaa ctctgtaaag
    53281 actatggggt atccgcttct gcgcttgcaa aatggatcaa acagtattct caagtcaaac
    53341 tcgaagataa ttctgtactt actgccaagc aaatacaaga gctacaaaaa cgtaatgcgc
    53401 agcttgaaga ggagaacctt atcctaaaaa aagcaagtgc catattcatg caaaacttaa
    53461 agtaagactc ctcgctgtct atcggttacg ctttgaacac gacacaacaa ccttatgtcg
    53521 tgttttacgt gtcaatcgct ccacttacta taaatttcta aaacataagc cctcaaaaag
    53581 agaattggat aatcaaattt atagaaaaca aatacttgag atttatacca aagcaaacaa
    53641 aagacttggt gtgaagtcta tcaaggtcat tcttcaaaga gactacgaca caaaaatctc
    53701 tgaaggaaga atttaccgtc ttatgaagaa tatggcgctc cctaaaatgg ctaccgttaa
    53761 gccaaaaaca gctcttaaaa ggactcaaaa aacgtatcct caaaacttac tcaaccagaa
    53821 atttaatcct gacaaaccaa atcaagtatg gtctactgac ttcacctata tttctattgg
    53881 atataagaaa tatgtctatc tctgcgcaat acttgatctc tattctagaa aatgtattgc
    53941 ttggaaactg agtcatcata tggatgcaaa gttagcatgt gacactctag aattagctct
    54001 taataaaaga aagattgaag gaacacttct ctttcattcc gaccaagggt cacaatttaa
    54061 ggccaggaaa ttgagaaaaa taattgatga caacactatc atgcattctt tttctaaacc
    54121 tggatatcct tatgataatg ccgtaactga agcatttttc aagtatttaa agcatagaca
    54181 aatcaaccga aaaaagtatc aaaatatcaa acaggttcaa ttagactgct ttgaatacat
    54241 tgagaatttt tataacaatt acaacccaca tacggctaat ctaggactaa cccctaatca
    54301 gaaagaagaa aattagttta acgtaataaa ataacacggt tttctgtcta cttatttgac
    54361 attagtccaa aatacatcct aatggtgtcg ttttttttat ttcactagac attcgttcta
    54421 gtttaggata taagaaggtc tcaaattttt ttcatgatgt ttattccaaa catccaatca
    54481 cctttctata taactgttca tttagtggta tagacaaaca gaaatactcc gtttctagtt
    54541 tcatagaaga cggagtattt tttagtagag acgtctggat tgatagatag ttggtatata
    54601 gctcatccct actaggcttt cattatagac tatggtgggt ttcgttgaca acaatatgtg
    54661 attattattc taaatga
//
LOCUS       DATUYR010000005        53474 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.005, whole genome shotgun sequence.
ACCESSION   DATUYR010000005 DATUYR010000000
VERSION     DATUYR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53474
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.005"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            101..538
                     /locus_tag="VCI33_000245"
     CDS             101..538
                     /locus_tag="VCI33_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEP2831201.1"
                     /translation="MNQNISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKT
                     LIGRLVEKKALGTYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTHLLLERLYD
                     LPMTLEEIEAFQELLEVKKENAVLEVSCNCLPGQCHCQKKETY"
     gene            538..2769
                     /locus_tag="VCI33_000246"
     CDS             538..2769
                     /locus_tag="VCI33_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEP2831202.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKMVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPPFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGYP
                     NMDSLVALATSFAFAYSLYGVVEISLGQANFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATVVRVWRNSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGPGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADKVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQKLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNAALMGILGIDLSCVQSVCAKASEKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALHQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVIADLRSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            2783..2986
                     /locus_tag="VCI33_000247"
     CDS             2783..2986
                     /locus_tag="VCI33_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEP2831203.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGQAKVT
                     GRPLTFLLKRALKDTKFELHQSS"
     gene            complement(3190..3960)
                     /locus_tag="VCI33_000248"
     CDS             complement(3190..3960)
                     /locus_tag="VCI33_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP2831204.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            4197..4670
                     /locus_tag="VCI33_000249"
     CDS             4197..4670
                     /locus_tag="VCI33_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2831205.1"
                     /translation="MFPNILFTEARTQPELFDLVASYLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            4663..4968
                     /locus_tag="VCI33_000250"
     CDS             4663..4968
                     /locus_tag="VCI33_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2831206.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPTVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            4994..6445
                     /locus_tag="VCI33_000251"
     CDS             4994..6445
                     /locus_tag="VCI33_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEP2831207.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYADHNHLPVLVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            6702..7127
                     /gene="lacA"
                     /locus_tag="VCI33_000252"
     CDS             6702..7127
                     /gene="lacA"
                     /locus_tag="VCI33_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP2831208.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            7142..7657
                     /gene="lacB"
                     /locus_tag="VCI33_000253"
     CDS             7142..7657
                     /gene="lacB"
                     /locus_tag="VCI33_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP2831209.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVNGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLESHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            7667..8120
                     /locus_tag="VCI33_000254"
                     /pseudo
     CDS             7667..8120
                     /locus_tag="VCI33_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            8167..9144
                     /gene="lacD"
                     /locus_tag="VCI33_000255"
     CDS             8167..9144
                     /gene="lacD"
                     /locus_tag="VCI33_000255"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP2831210.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            9341..9958
                     /locus_tag="VCI33_000256"
     CDS             9341..9958
                     /locus_tag="VCI33_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEP2831211.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAVVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            9960..10784
                     /locus_tag="VCI33_000257"
     CDS             9960..10784
                     /locus_tag="VCI33_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP2831212.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(10820..11377)
                     /locus_tag="VCI33_000258"
     CDS             complement(10820..11377)
                     /locus_tag="VCI33_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP2831213.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            11670..12512
                     /locus_tag="VCI33_000259"
     CDS             11670..12512
                     /locus_tag="VCI33_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP2831214.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(12641..13363)
                     /locus_tag="VCI33_000260"
     CDS             complement(12641..13363)
                     /locus_tag="VCI33_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831215.1"
                     /translation="MATMKQYHKGHSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            13565..15196
                     /locus_tag="VCI33_000261"
     CDS             13565..15196
                     /locus_tag="VCI33_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEP2831216.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKLFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADGKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            15313..16461
                     /gene="nagA"
                     /locus_tag="VCI33_000262"
     CDS             15313..16461
                     /gene="nagA"
                     /locus_tag="VCI33_000262"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEP2831217.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLGLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKTPRLEEFDAWQKAAK
                     GLIKKIALAPEREGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            16583..17422
                     /locus_tag="VCI33_000263"
     CDS             16583..17422
                     /locus_tag="VCI33_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP2831218.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDRLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            17554..18087
                     /locus_tag="VCI33_000264"
     CDS             17554..18087
                     /locus_tag="VCI33_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEP2831219.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            18459..19376
                     /gene="glyQ"
                     /locus_tag="VCI33_000265"
     CDS             18459..19376
                     /gene="glyQ"
                     /locus_tag="VCI33_000265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEP2831220.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            19754..21793
                     /locus_tag="VCI33_000266"
     CDS             19754..21793
                     /locus_tag="VCI33_000266"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEP2831221.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQCVIVDAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGNFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYTNKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAVAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRVAILSDLVSKAKTIAAFNQLNTK"
     gene            21947..22204
                     /locus_tag="VCI33_000267"
     CDS             21947..22204
                     /locus_tag="VCI33_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEP2831222.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            22331..22723
                     /locus_tag="VCI33_000268"
     CDS             22331..22723
                     /locus_tag="VCI33_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEP2831223.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            23083..24609
                     /gene="glpK"
                     /locus_tag="VCI33_000269"
     CDS             23083..24609
                     /gene="glpK"
                     /locus_tag="VCI33_000269"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEP2831224.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQLA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            24625..26463
                     /gene="glpO"
                     /locus_tag="VCI33_000270"
     CDS             24625..26463
                     /gene="glpO"
                     /locus_tag="VCI33_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEP2831225.1"
                     /translation="MEFSRETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDETRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGQQEG"
     gene            26465..27166
                     /locus_tag="VCI33_000271"
     CDS             26465..27166
                     /locus_tag="VCI33_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP2831226.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            27275..28621
                     /locus_tag="VCI33_000272"
     CDS             27275..28621
                     /locus_tag="VCI33_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP2831227.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLYFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDGDLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            28611..30110
                     /locus_tag="VCI33_000273"
     CDS             28611..30110
                     /locus_tag="VCI33_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2831228.1"
                     /translation="MKIEDLMDKERKAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYLEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFSQQLSDDILALLVFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVYLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            30236..30880
                     /gene="fsa"
                     /locus_tag="VCI33_000274"
     CDS             30236..30880
                     /gene="fsa"
                     /locus_tag="VCI33_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP2831229.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            31098..33083
                     /gene="tkt"
                     /locus_tag="VCI33_000275"
     CDS             31098..33083
                     /gene="tkt"
                     /locus_tag="VCI33_000275"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEP2831230.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVNHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAVYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNFNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLEGILIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            33276..33542
                     /locus_tag="VCI33_000276"
     CDS             33276..33542
                     /locus_tag="VCI33_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP2831231.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            33576..34310
                     /locus_tag="VCI33_000277"
     CDS             33576..34310
                     /locus_tag="VCI33_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831232.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            34315..35943
                     /locus_tag="VCI33_000278"
     CDS             34315..35943
                     /locus_tag="VCI33_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831233.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQENHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVIQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKAKQTKKPVNRPHQNQSLTQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISILLSCLTLMVLGIAHLWIQKTFWKSLEKL"
     gene            36007..36828
                     /gene="proB"
                     /locus_tag="VCI33_000279"
     CDS             36007..36828
                     /gene="proB"
                     /locus_tag="VCI33_000279"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEP2831234.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            36821..38071
                     /locus_tag="VCI33_000280"
     CDS             36821..38071
                     /locus_tag="VCI33_000280"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEP2831235.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATNIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            38091..38312
                     /locus_tag="VCI33_000281"
     CDS             38091..38312
                     /locus_tag="VCI33_000281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831236.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFDLFGDGM
                     LVSWIKRRKMSKVNQLLIDLQVTLQNCRR"
     gene            38355..38519
                     /locus_tag="VCI33_000282"
     CDS             38355..38519
                     /locus_tag="VCI33_000282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831237.1"
                     /translation="MSWDKIFDIWLDNLLTDCCVIKEAKNFLQVVEILEAAAKSVLAK
                     LADSEQKEQD"
     gene            38543..39556
                     /gene="rsmH"
                     /locus_tag="VCI33_000283"
     CDS             38543..39556
                     /gene="rsmH"
                     /locus_tag="VCI33_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEP2831238.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            39561..39884
                     /gene="ftsL"
                     /locus_tag="VCI33_000284"
     CDS             39561..39884
                     /gene="ftsL"
                     /locus_tag="VCI33_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEP2831239.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            39888..42143
                     /locus_tag="VCI33_000285"
     CDS             39888..42143
                     /locus_tag="VCI33_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP2831240.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            42145..43155
                     /locus_tag="VCI33_000286"
     CDS             42145..43155
                     /locus_tag="VCI33_000286"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEP2831241.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            43313..44656
                     /locus_tag="VCI33_000287"
     CDS             43313..44656
                     /locus_tag="VCI33_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2831242.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERNAKKQHF"
     gene            44851..45654
                     /locus_tag="VCI33_000288"
     CDS             44851..45654
                     /locus_tag="VCI33_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP2831243.1"
                     /translation="MTSVFLTSGWAFYDYLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKFLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            45654..46397
                     /locus_tag="VCI33_000289"
     CDS             45654..46397
                     /locus_tag="VCI33_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2831244.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYSRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            46501..46725
                     /locus_tag="VCI33_000290"
     CDS             46501..46725
                     /locus_tag="VCI33_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEP2831245.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            46789..47706
                     /gene="trxB"
                     /locus_tag="VCI33_000291"
     CDS             46789..47706
                     /gene="trxB"
                     /locus_tag="VCI33_000291"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEP2831246.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDQEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            47875..49254
                     /locus_tag="VCI33_000292"
     CDS             47875..49254
                     /locus_tag="VCI33_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP2831247.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            49425..50879
                     /locus_tag="VCI33_000293"
     CDS             49425..50879
                     /locus_tag="VCI33_000293"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEP2831248.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPGNFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKTYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMTLIDNIRKDTHGKSE"
     gene            50881..51705
                     /gene="nadE"
                     /locus_tag="VCI33_000294"
     CDS             50881..51705
                     /gene="nadE"
                     /locus_tag="VCI33_000294"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEP2831249.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKAAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            51890..53227
                     /gene="pepC"
                     /locus_tag="VCI33_000295"
     CDS             51890..53227
                     /gene="pepC"
                     /locus_tag="VCI33_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEP2831250.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQC"
BASE COUNT        16056 a         9631 c        11685 g        16102 t
ORIGIN      
        1 caacttaaga aataaaatca ttagaaactt tatatattta caaatgtaga taaatatgat
       61 ataatatatc tacaaatgta gattaaaagg ttgtgagttg atgaatcaaa atatttcagc
      121 tgccgaatgg gaagtcatgc gtgtggtgtg ggccagtggt gatattaaaa gtagtgatat
      181 tattactatt ttaaggaaaa aatatcaatg gtcagattct accattaaaa ccttgattgg
      241 gcgtttggtg gaaaaaaagg ctttgggaac ttaccggcaa ggaagagctt atatttatca
      301 agctcttttg gatgagactt tgctacaaaa agaagcacta gcgactgttt tagacggtat
      361 ttgtcagcgg caacacacgc atctacttct tgaacggctt tatgacttac cgatgacctt
      421 agaggaaatt gaggcattcc aagagctact agaagtcaaa aaagaaaatg cagttcttga
      481 ggtgtcgtgt aattgcctgc ctgggcagtg ccactgtcag aaaaaggaga cgtattaatg
      541 gctaaagaga tatttttagt ggagggaatg agttgtgcct cttgtgctct aacaattgaa
      601 aaaatggtca atcaattgcc tgaagtagaa caggctgttg tgaatttagc gaccgagaaa
      661 ctcacggtta cttatcaaga tgacgattta gaaacggcca aagttattca agcggtaaaa
      721 gctgctggtt atggtgcaca agtctttgat gaacatgcaa aacacatgca aacaggtcgt
      781 cgtgacgaag acctaaaggt tatttggtct caggtcttgt ggtctgccct attcaccatt
      841 cctatattat acctagctat gggtcacatg gtgggcttat ggcttcctcc atttctgcaa
      901 ccagatcgct atcctttcgt ttacagcttt agtcagctac tcttgacgat tccagtctta
      961 gtattaaatc gtcattatta ccataatggg tttaaagctt tgtttaaggg atatcctaat
     1021 atggactcct tggtagcttt agcaaccagc tttgcttttg cttatagtct ttatggagtg
     1081 gttgaaatca gtttgggcca ggccaacttt gtgcactccc tttattttga atcggtggta
     1141 gtgattttga cactcattgg tctaggaaag tattttgaaa gtcgttctaa aggccgtact
     1201 tcccaagcta ttcaaaagct cttatccttg aaggctactg ttgtgagggt atggagaaat
     1261 agccaatggc aattgttgcc gttagaagag gttagctatg atgaccttgt cctggttcaa
     1321 ccaggtgaaa aagtttctat tgacggtatt atcgtggaag gacatagcag tttggatgaa
     1381 tcctttttaa ctggggaaag tttgcctgtt gaaaagggag aaaaagataa tgtctttgcg
     1441 ggcagcttga atgggccagg cgccctcacg attaaaccag ataagcttgg gaatgatacc
     1501 ttgttagcac aaattatcca actagttgag aatgctcaag aaaataaagc acctattgca
     1561 gctattgctg ataaggtctc aggtgtcttt gttccggtgg ttttggtatt agctctgtta
     1621 acaggggtgt tttggttggt attcatgcag gaaagtcttc gttttagttt gacaacagcc
     1681 atcgctgttc tagtcattgc ttgcccttgt gcgcttggtt tagcaacgcc gacagcgatt
     1741 atggttggaa cagggcgtgc agctgaaaat ggtattcttt ttaaaggtgg tgacatctta
     1801 gaacaagccc atcaggtaga tacggttgtt tttgataaaa cggggacgat tacggagggg
     1861 aaaccaagtc ttcaaaagct cctaactttt tcgggagatg atcaccttat cttgcaagaa
     1921 gctgcttcct tggaggccta ttctcaacac cccttagggg aggctattgt tcgtgctgct
     1981 caggaagctg gttacgatag tctaccagtg gaagagtttg agtctttaac cggcctagga
     2041 gttactggtc agcttgaagg gcgtcgtttg gcgattggaa atgctgcttt aatgggaatt
     2101 cttggtatcg atttatcttg tgttcagtca gtttgtgcta aggcttctga gaaaggccaa
     2161 acccttgtat attatgctaa agaggggcag ctacgagctc ttttcagtat tgctgacgct
     2221 gtcaaagaag atagtcaagc taccgtcgaa gctttgcatc aactgggaat tcacaccatc
     2281 atgctaacag gtgaccacga tgctaccgct aaggctattg cctctcaagt ggggattaca
     2341 gatgtgatca gtcaagtctt acctgatcaa aaagcaggcg tgatagcaga cctccgctct
     2401 caagggagaa aagttgcaat ggtaggtgat ggtattaatg atgctccggc attggctgta
     2461 gcagacattg ggatagctat ggggtcaggg acagatattg ccattgaatc ggcggatgtg
     2521 attctgatga aacctgatat gctggatttg gtgaaagcta tgtcattgag tcgagcgact
     2581 atgaggattg tcaaggaaaa tttattttgg gcattcattt acaatgtcct catgattcca
     2641 gtggccatgg gcttacttca tctttttgga ggcccattat tgaacccgat gttagcaggt
     2701 tttgccatga gttttagttc agtatcagtt gttttgaatg ccctgcgatt aaaaggaaaa
     2761 accatttaaa ggagaataca aaatggaaaa acattatcag gttacaggaa tgacatgtga
     2821 cggttgcgca agaacggtca ctgaaaaatt atcagctgtc cccggtgtgc aatcggtcca
     2881 ggtaaatttg gaaaaaggac aggctaaggt gacaggacgt cctttaacgt tcctactcaa
     2941 gcgtgctttg aaggatacca aatttgaatt acatcaatca tcttaggtta ttttggtagc
     3001 cttacttcat ctcaaaagag gttccagttt tttaatctgg gcttcttttt ttgtggtcac
     3061 tcataaagac tctgacgtta gcaggtgtgt tccgagcaaa ataaaaacac ctctctggaa
     3121 gcaaatcact tttgccagaa ggtgtttaag gacgatgcgc tgacaggctg tccattcttg
     3181 atgaaacggt tactccttgt tttcgtgggc tactaataat tgtgtgaact gactataacg
     3241 ttcgatgacg tcctcagtta tttgcgaatc tgtaacaaca gcatcaatgt tgtttaaatg
     3301 gtaaaaaatg aagaagtcgt aggcattgaa tttttggtta tctaccaaga ggtatttttc
     3361 aatagaatta ttaaaagcta gcttttgaat ctctccttca gtttcgctat aagttgcaat
     3421 atcgttttta taaataccat tacagctaat aaaagccttg gcaaatttta aggatgagat
     3481 attttgactg gcaagggacc ctacaaaagc tcctgtaata gatcggtatt caccgccgat
     3541 aagaatcagg tcaatggttt cgctgtcctg aaggatgtta aagacaggga ggctattggt
     3601 cacaatgcga atctgacgat tttttaattg ctcagcgaaa cattctaaag ttgttcctgg
     3661 accaataaag attgtttcac catcattgac tagttgtgtc gcatatgaag ctagttccca
     3721 tttttccttt gtttgaacgg tttgtttttc aatatttgac cgctcttttt tattaggcat
     3781 ggtaatgctt tgtgcacctc catggatgcg gatcaatttg ccggcttttt ccagttcatc
     3841 taagtctcta cgaactgtca tatcagaaac gtttaaagtc tgaataatat cattcacagt
     3901 aacaatacct tgctcattga ctatttcggt aatttttaga agtctttctc tttttaacat
     3961 ctaaggggct cctttctata gtttatttac ttatgtttat catagcatat agaccttcat
     4021 atttcaataa aacaaaataa acaaaacaaa aacaaacata tagaacaaaa aatattgcgt
     4081 aaaaatgttg aattatgttg aaggttatac tataatgatt ttgtaaacgg ttaccaattt
     4141 gttgatgatg agatgacagt tgttaaaaat tgatacttat agaaaggcgg tagggagtgt
     4201 ttcctaatat tctatttact gaagcaagga cgcagccaga gctttttgat cttgttgcta
     4261 gctacttgga aaaggttggg tatgtgacgc aagagtatca tcaggcttta gtggagcgtg
     4321 aggcggtatt tccgacagga ctcaaagtgg acctcaaaga tgggtcagat attttgtatg
     4381 cggctattcc gcatacagaa accaagtatt gcttggttga tcaagtggtc tatgttagaa
     4441 atagtcaagc ccttaccttt aagcacatga ttaatccaga ggaagactgt ttggtaacgg
     4501 atttcttctt tattatcaat agtcaaaacg agggtcaaac gacgattcta tccaatctca
     4561 ttaccttctt tattacgaaa ggcaatctta gctatctagc cagtctaaaa gatgataaac
     4621 aagctatttc aaactattta atagaaaaag gagtgtttca gcatgattaa aattctagca
     4681 gcctgcggtg caggggttaa ctcaagccat caaattaaag atgcgattga aactcagatg
     4741 agtgatcgtg gctatgatgt gcattgtgat gcggtgatgg tcaaagacat cacagaagaa
     4801 ttggtgtctc gttatgatat ttttacgccg attgcaaaaa ctgacttggg atttgagatg
     4861 ccaattccca cagtagaagc aggaccgatt ctctatcgta tcccaatcat gagtgagcct
     4921 gtttttgctg aattagagcg cgtgattaaa gaaaaccatt tgcagtgagt taataaaaac
     4981 ataaggagac aagatgatgc aacctttttt agacattatt aacaagattc tgggatttcc
     5041 tattcagttg ggatcaggtg tggtcatgct gattgtcatg acaggtttag ccatgatttt
     5101 cggggttaaa ttcacaaagg cactggaagg cgggattaag ttagctatcg ctcttacagg
     5161 tattggagcc attattggga ttttgacggg ggctttttca gagtctttgc aggcctttgt
     5221 taaaaataca gggatcagtt taaacattat tgatgtcggt tgggcaccgc ttgcaacaat
     5281 tacctggggt tccccttata ccctttattt cttgctggtg atgctggtgg ttaatattgt
     5341 catgattgtc atgaaaaaaa cggacaccct tgacgtcgat atttttgata tttggcattt
     5401 gtctatcaca ggtctattga ttatgtggta tgctgatcac aaccatttgc cagtccttgt
     5461 gtctcttttg attgctactg tggcagttat tcttgttggg gtattgaaaa tcattaactc
     5521 tgatttgatg aaaccgacct ttgatgacct tcttgggaca gggtctcaat caccaatgac
     5581 ttcaactcac atgaactaca tgatgaatcc aatcattatg gtcttagata agatttttga
     5641 caaggtcttt ccggggctag ataaatatga ctttgatgct gctaaattga acaaaaaaat
     5701 tggcttctgg ggctctaagt tctttatcgg aatggctcta ggttttgtga ttgggattat
     5761 gggagatcct catttcactg ttgaatctat caaaaattgg tttggtctag gatttacagc
     5821 tggtgcctgc ttggaacttt tctctttgat tggctcttgg tttatcgcag ctgttgagcc
     5881 tttgtcacaa gggattacca attttgccaa tgctagaatg catgggcgtc gtttcaatat
     5941 cggtttggac tggcctttca ttgctggtcg cgctgaaatc tgggcctgtg ccaatattct
     6001 tgccccaatc atgttgattg aagccgttct cctatcaaaa gttggcaatg ggattttacc
     6061 attggcgggg attattgcta tggggatgac accagctctt cttgtggtca cacgtggtcg
     6121 tttgattcgc atgattattt ttggttccct cttattgcca ttattcctct tgtcaggaac
     6181 tatgattgct ccatttgcga cagagttggc taaaaaagta ggggcttttc cagcaggtac
     6241 aagcgctggt tccttgatta cgcattcaac acttgaagga ccaatggaaa aaatttttgg
     6301 ttatgtgatt ggtcaagcga caacaggtca gattgcctca atcatcacgc taatcatctt
     6361 tgtggccatt tacctaggtc tctttgcttg gtatgctaac caaatgaagg cacgtaacgc
     6421 tgagtatgcg aaaacaatga aataaaatca ggtcaggtga ctgactaaaa aattagggag
     6481 aatagcagat aactgcgtaa ggtatttctg ccttaccccc taacgtgaaa agagtatttc
     6541 tcatgtaagg caaggcgctc tgtgggaact cacaagtgct ttgtcttgaa agaaataatg
     6601 gaagacacta agactactgg caatcatctt gatctaagga caggtaatca tgtccccctt
     6661 ctctagattg gcaagcgttt tgggttagaa aaggaaaagc tatggctatt attttaggtg
     6721 cagatgcaca cggaaatgca ttaaaagaat tgatcaaaag ttttcttcaa gaagaaggct
     6781 atgatattat tgacgtgact gatattaaca gtgattttat tgataacaca ttagcggtgg
     6841 cgaaagccgt taatgaagca gaaggtcgtt taggcattat ggtagatgct tacggagcag
     6901 gtccctttat ggtagccaca aaattaaaag gaatggttgc tgctgaagtg tcagatgaac
     6961 gctctgctta catgacgcgc ggtcacaaca atgcccgcat gatcacaata ggggcagaga
     7021 ttgtaggtcc agaattagcg aagaatatcg tcaaaggttt tgtgactggt ccctacgatg
     7081 gtggtcgtca tcaaattcgt gttgacatgc tcaataaaat ggcctaaagg aggataaagt
     7141 gatgaaaatt gcgattggtt gtgaccatat tgtcaccaac gaaaaaatgg ctgtttctga
     7201 ttttttgaaa tctaaaggct atgacgttat tgactgtggc acttatgacc atacaagaac
     7261 ccattaccct atttttggaa aaaaagtcgg cgaagctgtg gttaatggtc aagcagactt
     7321 gggtgtttgt atttgtggaa ctggagttgg cattaataat gccgtgaaca aggtaccagg
     7381 tattcgttca gcccttgttc gagatatgac aacagcgctt tatgccaagg aagaattgaa
     7441 tgccaatgtg attggctttg gtggtaaaat cactggtgaa cttcttatgt gtgatattat
     7501 agatgctttt atcaaggcag aatacaaaga aactgaagag aataagaagc taattgctaa
     7561 aatagcccac ttggaaagtc atcatgccaa ccaagaagat cctgattttt ttacagaatt
     7621 tttggaaaaa tgggatcgtg gtgagtatca cgattaggaa gaagagatga gtttaacagt
     7681 tatcttaaat ccttctgtcg ctatttccta tcctttagat tgtctggcta tggacacggt
     7741 taatcgtgtt gacagaacgg caaaaacagc tggtgataag gagctaaatg tgaccagaat
     7801 gttagcaaaa gctggtcagt tatggcaaca ggatttagtg gtggtaaact gggtgacttt
     7861 atcattcatc agttacaaga acagggcatt tctaaccaat ttttcaaaat caaaggggaa
     7921 acgagaaatt gtattgctgt tttacatgaa ggcatgcaga caaaaatttt ggaagctggt
     7981 ccctacagtg atgtggacga agccgaagtc tctttgagcc acatgagtac tattgctaag
     8041 ccgtttgatg tcttaacttc tcaggcagtc tgcctaggag actagctact cactattatc
     8101 aagatttgat gactatggca acttttaaac aattatatca gcaacttcaa gtaagagagg
     8161 tatcacatga caatcacagc aaataaaaga cattacttag aaaaagtgag ccatcaagga
     8221 atcatatcag ctttagcttt tgatcaacga ggtgcactga agcaaatgat ggcagcacat
     8281 caagaaggag aagcgacagt tacacagata gaaacattga aagttctcgt ttctgaggaa
     8341 ttgacgcctt atgcttcatc tattttatta gatccagaat atgggttgct ggctactaag
     8401 gtaagagcta atcagacagg cttactgtta gcttacgaaa aaacaggtta tgatgctaca
     8461 accactagcc gtttacccga ttgcttagtg gaatggtcgg tcaaacggtt gaaagcagca
     8521 ggtgcagatg ctattaaatt tttgctctat tatgatgttg atggagatga acaaatcaat
     8581 ttacaaaaac aagcttatat tgaacgcatt ggttcagaat gtacagctga agacatccct
     8641 ttttttcttg aacttttgag ttatgatgag agaatatcag ataacaacag tgcggcatac
     8701 gcaaagctta agccacataa ggttaacggt gctatgtcag tattttcgga taagcgtttt
     8761 ggcgttgatg tgctaaaagt agaggttcct gttaatatgg cttatgtaga aggttttact
     8821 gaaggggaag tccattacag tcaagctgag gctattaaag cttttcaaga ccaagaagct
     8881 gccagccacc tcccttatat ctatttgagt gcaggcgtat ctgccaaact ttttcaagag
     8941 acattatact ttgcagcagc agcaggtgct caatttagcg gtgttttgtg tgggcgtgcc
     9001 acgtgggcag gatccgttcc agtttatatt actaaaggag aggacgaagc aagaaagtgg
     9061 ctatgcactg aaggcttcca aaatattgac gagttaaaca gggtactcga agagacagct
     9121 agcccttgga cggaaaaaat ctaaaacaac cttatcctat caagcagctc tcattgatgt
     9181 gataagataa gtatggggat aattgtttga atcttttgta gcaaggatga aaatgggttt
     9241 tatccttgct tttttatcct tacattttat ctttttctac ttatttttta aaggaaaacg
     9301 tggtaaacta aaagaagact aaaagacaat agggtagctt atgacaattg atatgtggga
     9361 aattttaaat atcatcggga ccattgcctt tgctctttct ggtgctattg ttgccatgga
     9421 agaagaattt gatattttag ggatttttat tttaggtttc gtgacagcct ttggaggcgg
     9481 agccattcga aacaccctaa ttggtttacc tatagaggcc ttatgggggc aaaagccaga
     9541 attcacttgc gctttctttg ctatggtttt gattatgctt tttcctaagc taatagctag
     9601 aggttgggta cgggcagttg ttttgactga tgccattggt ctggcagctt ttagtgtcca
     9661 aggagccttg catgctgtcc gtcttaacca gcctttaagt gctgtcattg tagcagctgt
     9721 gttgactggt gcaggcggtg gtgttgtcag agatattctg gcagggcgaa aacctagtgt
     9781 tttgcgtagt gaaatctatg ctgggtggtc tattcttgca gctattgttt tgcattttaa
     9841 actagcagat tcaacgattg aatgttatgc cttggttgtt ttactcacga ctttacgcat
     9901 gattggcaac cgtaaaaaat ggaacctccc taaaatcaaa tggaaagagg aaagatagga
     9961 tgattcaact aattgcaatt gatttggacg gcaccttatt aaaccaggac aaacagattc
    10021 caaaagagaa tatcacagcc atccaagaag cagctcagtc aggcttaaaa attgtcttat
    10081 gcacaggccg gcctcagtca gggacaagac cctattttga ccaattaggt ttgacacaag
    10141 aggaattcct cattatcaat aatggctgta gtacttatag tagtcctgac tggcaattac
    10201 gccattcaaa aatgttgaag gtttcagata ttgaattgct ggaagagcta agccaaagtt
    10261 ttccagatat ttacctgact ttaactgagg aaaacgatta tttggtgctt gaagaagaag
    10321 tgccagacct tgtgcaggaa gatggagatt tggtatttac cattgtcaag cctgtctcct
    10381 tagcagagct gtcggacact cctcgcctga tttttcaagc catgtactta ggagaaaaag
    10441 ctgcgctaga tgcctttgaa cgtgcggtga gaaatcagct tagtcagagt tttcatgttg
    10501 ttcgtagcca agataatatt ttagaaattc tccctcaggg agtcagcaag gcatcggctc
    10561 tcaaagaatt agtagaagat ttgggtttaa ctgctgacca agtgatggct attggggatg
    10621 ctcccaatga cattgagatg ttaacttatg ctggtttagg agttgctatg gaaaatgctt
    10681 cagcagctat caagccatta gctgacaaag tcactttgac taatgatatg gcgggtgtgg
    10741 cccaggctat ccgtcagttt gcacttgcag ctcaaaaaga ctaactatta tataaaaaag
    10801 gttcccaaaa ggaacctttt tacgttaata gaggcaagcc catagcagcc atttcttcgg
    10861 caggaacgat catgtcatct gaaatccagt ttgtcaagac agaaacaata gcattgctcc
    10921 aaaaaaggcg cttatagtgc gattgttttt tgtcttttaa tttttgataa gctttcagac
    10981 gtttagtgac taatcgtgtt agaagatggt gcaggtgatg tttgaaaatc attttaagaa
    11041 gctgagcttc ttttttagct tcagtaaaaa gatagagcca ggcttgataa gcctgagttt
    11101 taatatcaaa ttgttttagt ccacgaaaaa tacgacggat aacccctact aacagttgtt
    11161 ctataatggc ttctttagag gtataattac gataaaaagc gttacgagat actcctgctt
    11221 tcgtcaccaa ttctgaaatc gtaatatcac ctaaaggttt tgtttctaga agattcagta
    11281 aggcgatttc catagattca cgtgctatgc gtcgtgtttc tttattaaat tgtgttaagt
    11341 tttgaagtga tctttttgag agtgctttat cagtcatcac aaataccttt caagtgttac
    11401 ctctgatgag ttatcagtag caaaaaccga cttaataatg atataatttt atgggaaaag
    11461 tcttagtttg tcaactaaag ttagttatca ggagcctcat tacctccttg tatagctcta
    11521 ttggatgact ttactaacta ttgaaaaggc tagtaataaa acataggatt taatgattag
    11581 ttattaccat tagcctagac atcttagcga aggcttaata atctggtctt tgttaccgaa
    11641 cacaaattag agaaaaagaa ggataaaaaa tgacttggaa aattgtaaca gattcaggat
    11701 gtgatcttag aagtttgact agacaatcca aagagttgcg ctttgaacgt gtgcctttga
    11761 ccctacagat tggcacagaa atttttagag atgatgatgg attagatatc gataatatga
    11821 tgacaactat gtaccagtcg tctaaagcaa cgacatctag ctgtccaagc ccagaagcct
    11881 ttttgcaggc ctatagaggt gcagataatg ttatcgtgat gacaattaca gggaccttat
    11941 ctggcagtca taatagtgca cgactggcta aaaatgaact tcttgaagaa aatcctaatg
    12001 tcaatattca cctaatcgat tctttatcag caggtggcga gatggatttg ctcgtactgg
    12061 agcttgagcg gttgatcaat cttggattat cctttgaaga agttgtcaaa cagatcacag
    12121 cttatcagca aaaaacacgt ttgatttttg tgcttgccaa ggttgataat ttggttaaaa
    12181 atggtcgact gagtaaatta gtaggtaaag taattggctt actcaatatt agaatggttg
    12241 ggaaagctag caataaagga acgcttgaac ttcttcaaaa agcgcgtggt caaaaaaaag
    12301 cagtttctgc tttgatagaa gaaattcaaa aagagggtta tgtaggcgga aaagtttaca
    12361 ttgctcatgc tcaaaatcca aaaatttgtg agcagatcag tgaaaaaatc aaatctttat
    12421 atcctgatgc tgtgattcaa actggaagga cctcaggttt gtgtagcttt tatgctgaag
    12481 acggaggact tttaatgggc tacgaaattt aatgctatct aacaaaacaa aaagctagac
    12541 tcttttgatt tgagctagct tttttaagat atcaggaaga ctaatcacta agatagccgt
    12601 ctaaaacgtt tattgattga ttttacgaaa aatttttgta ttactctttt gatgattgct
    12661 tgtctcctga gtcagaaggt tctgaagagg tctgaggtgg aattggatca ggatgactag
    12721 gttgtggcgt aacatctgaa gctgaagatt ctgagcttgg gtctaaagta ggtgactggg
    12781 aattatcata agtatctgag acgtctcctg ggaaaggtgt ccagtaagag ttatctgtta
    12841 tttctccact ttgtcttgga aattcatcaa taatgggttt tgctggtaaa atagcgtcta
    12901 accgagattg taaagcggct tttttacctt tgttacttaa gtcgttaact gctttttggg
    12961 cgatatcttt aagattttga ttgagagtct cctcagcagc tttaactgct tgagtagctg
    13021 catcttcttg ctcttgcttt gctttggcaa cgtttagctg ttcttgtagc gtttttttct
    13081 tagaagattc tgctaattta tcaacagctt tctgggcttc ttggatagca gctgcagtgg
    13141 ctaaacgatc agcgtcatta actgctttaa ctgctttttt tagtaaacta gcagcacttt
    13201 tagaatgttt agctacctta tcttgcgttt gagggtggga tccaaaaata aggcaggagc
    13261 cacctattag agtgatagtt agaacactca acatccaata aagcgcctct ttttgtgatt
    13321 tagtcatgga atgtccttta tgatattgtt tcatcgtagc catattatac ctctaattaa
    13381 acgaaatgaa aagatttgtg caaaaacaaa tgatgtaaaa tatcggaaaa gcctaacacc
    13441 ttatcaaacc aataatatgc aaatgttgac atcggtatac acagaaacgc ttaaaaaaac
    13501 cttgtgataa agaccgtaat acattaaaat agaatagact aaaaaattga gcgaggacaa
    13561 tattgtgtca gtaaattggc aagacattgc atttcatttc tttggaggct taggtctctt
    13621 tcttttcagt atcaagtata tgggagatgg tctgcaacaa gcagcaggag ataaactaag
    13681 gtattacatt gataaataca ccagtaatcc cttttttggt atcttagtcg gaattgccat
    13741 gtctgcctta attcagtcca gttcaggcgt aactgtgatt acggttggtt tggtttctgc
    13801 gggtttgcta aatttgcgac aagccattgg aattgttatg ggggctaata ttggaacgac
    13861 tatcacttcg tttttaattg gttttaaact aggagattac gccttgccca tgatttttat
    13921 tggtgcagcc tgtcttttct ttacctctaa taaaaagctc aacaattttg ggcgtatcat
    13981 ttttggtgtc ggaggaattt tcttctcgct gaacctaatg ggagatgcca tggatccttt
    14041 gaaatctgtg tcagcctttc aaaattattt agcgacatta ggtgataaac tcttccaagg
    14101 tgtctttatc ggaacggctt tgaccatgtt gattcagtca tcggcagcca ttatcgggat
    14161 tttacagggt ctgttttcag gtggtctttt gactttacag ggagctattc ctattttgtt
    14221 aggctcaaat ataggaacct gcattacagc tgtcttagca gctataggtt caaatattgc
    14281 tgctaaacgt gtggcagcag ctcatgtgtt atttaatttg atcggtacaa ttatttttat
    14341 gattatccta gtcccattca catcattgat gttatggcta caatcaaagt tgagtttaac
    14401 gcctgagatg actatcgctt tttctcacgg ttcttttaat atcactaata ccatcttatt
    14461 aattcctttc attagtctac tagctatgat tgtgactaga ctcattccag gagaagatga
    14521 agtggtcaaa tacgaagccc tttatttaga tcgcttactt attacccaag ccccttctat
    14581 cgcactagga aatgcccaca aggaattggt tcatttagcc tcttatgcga ttcaagcttt
    14641 tgaagcttct tacagttata ttatgacagc tgacggtaaa tttggggaaa aggtcaagcg
    14701 ttatgaaaga gcagttgata ccatcgatga ggagttaacc acttatttgg ttgatatttc
    14761 taacgaagcc ctcagcccaa gtgaaaatga agtcttggct ggtattcttg actcttcacg
    14821 tgatttggaa agaattggag atcattctga gtctttaggc attctaattg aaggcatcat
    14881 atctaaacaa attggttttt caatatctgc tcgtcaagaa ttgacagaga tgtatcaatt
    14941 gacgcattgt ttaacactgg atgccattag agctatcgtt gatagtgata cagacttggc
    15001 gcaaactatt gtgactcgcc ataaagaaat tgaagaaaaa gagcgacgat tacgtaaaac
    15061 ccacattaag cgcttgaatt gtggagaatg tacggcacaa gcaggtatta attttattga
    15121 tattatttcc cactataccc gcatcacaga tcatgccttg aatttagcag agaaagtact
    15181 ttcacaccag ctttaaaaaa gagaaaacgt gaatgatcat ccacgttttc ttttttgaat
    15241 ccaaaaagtg gtatatacca tttacaaagt aaagaataag gcgtataata aggaaaaaag
    15301 ggaggaaaaa agatgacatg ctatcttaaa gcagattgtt tttattaccc aacagaggtg
    15361 aaaccggctg gctacctagg tcttcatgat ggtgtttttg gggagtggac agaaattgtt
    15421 ccggcagatg cgcaaatcat tgactacacc ggttatcaaa ttgcaccagg tcttgtggat
    15481 acgcatatcc atggctatgc gggagctgat gtcatggata attctgccca agggattcat
    15541 caaatgagtg aaggactatt agcaacagga gtaacgtctt ttttaccgac gaccttaact
    15601 tctacctttg agcagttaga aaaagtatca gggactattg cttcagttgc tgatcaggta
    15661 aaaggtgcta aaatacaagg tatctacttt gaaggtcctt attttaccga agaatataag
    15721 ggtgctcaaa acccaagtta tatgaaaacc ccacgcttgg aggaatttga tgcttggcaa
    15781 aaagctgcta aagggctcat caaaaaaatt gcccttgctc ctgagcgtga gggtgtcaaa
    15841 gaatttgtgt ctgcagtgac aaaacaaggc gtaacagtgg ccttagggca ttcaaatggt
    15901 acttatcaag aggctaaaga agctgttcaa gctggtgcta gtgtttgggt tcatgcttat
    15961 aatggcatgc gaggattgac ccaccgtgaa ccaggaatgg tgggagcggt ttataatctt
    16021 ccaaatactt acgcagagtt gatttgtgat ggccaccatg tctcaccaat tgcttgtgat
    16081 attttgatgc agcaaaaagg gcatgatcat gtagccatga ttacggactg tatgcgagca
    16141 ggtggttcac cagatggtga ttacctcttg ggggaattct ctgtggttgt ggcaaatggc
    16201 actgctcgtc ttaaagagag tggaaacttg gcaggatcta ttttgaaatt aaaagacggc
    16261 cttagaaatg tagtggcctg gggaatcgca actccggcag aagccattca tatggcaact
    16321 tatgtgccag ctgtttctgt cggtattgac gacgtatgtg gtcaaatcaa agctgggcat
    16381 gcggctgact ttattgttct tgataaggat ttaacacttg ttgcaaccta tttaaatggg
    16441 caaaaagctt ttgatgccta agagatacaa aaggattgga gtagctatgt ctggcaggcc
    16501 aattcttttt gtattttctt tctttatgat agaatagatt aaagtaatcg cttacagata
    16561 ttgataaaag gaggtttttg tgatggtaac aacagtaaaa atgacaagtg gctatgagat
    16621 tcccgtttta ggatttggaa cttaccaagc agccgatggt gaggaagctt accaatctac
    16681 cttagcagcc atcaaggctg gctatcgtca tattgatact gcagctattt ataaaaatga
    16741 agaaagtgtg ggtcgtgcca tcaaggattc tggggttcta cgagaagact tatttattac
    16801 aactaaactc tggaatgacg cccatagtta tgaaggggca aaagacgctt tagctgcttc
    16861 tcttgaccgt cttggtctgg attatgtgga tctctatctt atccattggc caaacccaaa
    16921 agctctgcgt aacacttgga aagaggcaaa tgcccaagca tggcaatata tggaagaggc
    16981 ggttgaagca gggcttatta aaagcattgg cgtcagcaat tttatggtgc atcatctaga
    17041 agctcttcaa gagacagcta aaataactcc agcaattaat cagattcgat tagccccagg
    17101 ttgctatcaa aaggaagtgg ttgattattg taaggcaaat gagattttat tggaagcttg
    17161 gagtcctcta ggacaaggag agatttttga taatgagact atgcagcagt tagctaataa
    17221 atatgacaaa acagtggcac aagttgcctt agcttggtcg ctggcagaag gttttattcc
    17281 cttgccaaaa tcggtgcatg atgagcgcat caaggaaaat atggctattt ttgatgtgag
    17341 cttgacacaa gaagataaaa aaacaattcg ctatctctca ggaatgtcag cgattcctaa
    17401 ccctgatact acaagttttt aaggaactac aaagatttag ccataattcg ctaaatcttt
    17461 tttgaaaacg gtttgacaaa ggtttatcat ttagctataa tctttattag tgttttgtga
    17521 aagaaactat tattgaaaat aaggagccgt attgtgacca tctttttagt gatgttagct
    17581 atgtttacta ttcgtttagt atttttgaag aaatcgataa caaatgaaaa agccatatta
    17641 gcccaaggag gacaggaatt tggtgctcag aacacgaaat ttttgacgtt acttcacatt
    17701 atgatttatg tgtttgcggt gattgaagcg ctgcttaaac aaattaagtt tgatggcatt
    17761 agttttttag gtcttttgtt gatgttatta tcagtagctg ttttatatga agtgacacgt
    17821 atcttaggtg acatttggac tgtaaaattg atgttagcaa aagaccacaa atatgtggat
    17881 cactggttgt tcaaaacgat taaacatcct aactactttt taaatatcgc ccctgagctg
    17941 gtgggcatag ccttactttg ccatgctaaa attacggcta tgcttctttt cccatgctac
    18001 attgtagtaa tatatttacg tattcgagaa gaaaacaagc tattagcgga agtgattatt
    18061 ccaaacggta ctcgaacaaa accttaagct catgatggac aaactaatgg ctttatgcta
    18121 aactagactc taggaaaaag gatgcaagta tcttatctaa agcgagttcg gggtagttag
    18181 agccgaatgg taggactgca gattggcgct tccgtttggg cagtgtgatt aagtatattt
    18241 gtcaatattg cccaaaaaga tactatataa atgaagtaat aaattagggt ggaaccgcgt
    18301 tttgacgccc ctaggtctta tgatttagga gcatcaaaat gcggtttttt ggtgcctaga
    18361 cctgtaatac atgctagtaa catcgtcagg tattttactg atgacaaaaa aacgattgtc
    18421 aatgatatac cattttacta ctatcaaagg agtacgaaat gtctaaaaaa cttacctttc
    18481 aagaaatcat cttgacctta caacaatatt ggaatgatca gggttgtatg ctcatgcaag
    18541 cctatgacaa tgaaaaagga gctgggacca tgagtcctta cacgttctta cgtgctatag
    18601 gtcctgagcc atggaatgcg gcttatgtag agccatcacg tcgtcctgca gatggccgtt
    18661 acggagaaaa cccaaatcgt ctttaccaac accaccaatt tcaagtggtc atgaaaccat
    18721 caccatcaaa tattcaagaa ctttaccttg cttctttgga aaaattgggc atcaatcctt
    18781 tagaacatga tattcgtttt gttgaagata actgggaaaa cccatcaaca ggatcagcag
    18841 ggcttggttg ggaagtttgg cttgatggaa tggaaatcac acaatttacg tatttccaac
    18901 aagttggtgg ccttgcgaca tctccagtga cagcagaagt aacttatggt cttgagcgct
    18961 tagcttccta tattcaagaa gtggattcgg tctatgatat tgagtgggca ccaggtgtta
    19021 aatatggtga aatcttctta caacctgagt atgagcattc aaaatattcc ttcgaaatat
    19081 cagaccaaga catgctgctt gaaaactttg aaaagtttga aaaagaagcc tcgcgtgccc
    19141 tagaagaagg tttggttcac ccagcttatg attacgtgct caaatgttct catacgttta
    19201 acctgctgga tgcccgtggc gctgtttccg tcacagaacg cgcaggctac attgctcgta
    19261 tccgtaattt ggcacgtgtc gttgctaaga cctttgttgc agaacgcaag aagcttggat
    19321 ttccactgtt ggatgaagca acacgcgcaa tcttgctagc tgaagatgac gaataagtcg
    19381 atttaaagat gatctagttg cgagctttgt aatcttaaga gcgagcaaga agtgggcgac
    19441 cgaaggtcgc cgagtcaagg ttactcccgc tgtagtgaca attcggtaag gttacttatc
    19501 gaacgaggga gttggcgaga ctttaggctg gctaactagg aatggaacac ttttgttttc
    19561 tgaaattatc cactggataa tttcactttg cgtcccccct acctaaggga acaatcaaaa
    19621 agatactttt tagagaaagg aactgaacat gggctacgga ctatgtcaaa aagaccaaga
    19681 cttcctaggt gcaagcacct gcgtcgcttt gctattttga tggtgtccgc ttaacgccct
    19741 tggtatctta attatgagta aaaatctact aatcgaactt ggtctggaag aacttccagc
    19801 ctacgtggtc acaccaagtg aaaaacaact gggagagcgc ttggcgactt tcttaacaga
    19861 aaatcgtctc tcttttgaag atatacaaac cttttcaact ccacgccgtt tggcagtgcg
    19921 tgtgtctgga ttagctgatc agcaaacgga tttgactgag gactttaaag ggcctgctaa
    19981 gaaaattgcc cttgatgctg atgggaattt ctcaaaagct gctcaaggat ttgttcgtgg
    20041 caaaggcttg accacagatg ctatcgaatt ccgcgaagtc aaaggagaag aatacgttta
    20101 tgtgaccaaa cacgaagctg gaaaacctgc taaggaagtg cttctaggcg ttactgaagt
    20161 gttgtcagca atgaccttcc cagtcagcat gcactgggct aacaatagtt ttgaatatat
    20221 tcgtcctgtt cacaccttga cagttctgtt gaatgatgag gccttggaac ttgacttctt
    20281 ggatattcat tctggacgcg tcagccgtgg tcaccgtttt ttaggtactg aaaccactat
    20341 cacaagtgct gattcttatg aggctgattt gcgcagccaa tgcgtcattg ttgacgctaa
    20401 agagcgtcaa gagatgattg tggagcaaat caagacgctt gaagtggagc agggtgtgca
    20461 agttgacatt gacgaagact tgcttaatga agttttgaac ttggtcgagt ttccaacagc
    20521 tttcatggga aattttgagg ctaaatacct tgatgtccca gaagaagtat tggtaacatc
    20581 aatgaaaaac caccaacgtt attttgtggt acgtgatcag gctggtcact tgatgccaaa
    20641 ctttgtatca gttcgtaatg ggaatgatca agccattgaa aatgttatta aagggaacga
    20701 aaaagttttg gtagctcgct tagaagatgg tgaattcttc tggcgtgagg atcagaaatt
    20761 gcaaattgct gatttagttg ccaaattgac caatgtgacc ttccatgaaa aaatcggctc
    20821 tcttgctgaa cacatggatc gtacgcgtgt cattgccgca tcacttgcta aggaagccaa
    20881 cctatctgct gaggaagtca cagctgttga ccgtgctgcc caaatttaca aatttgacct
    20941 tttaacaggt atggttggtg aatttgatga gctgcaaggg attatgggtg aaaaatatgc
    21001 tcttctagct ggtgaagatg ctgctgtggc aacagcaatc cgtgaacact acttacctga
    21061 tgctgcagga ggtgcgctac ctgaaaccaa agttggcgct gtcctggcct tggcagataa
    21121 attggatact ctcttgtcat tcttttctgt tggattgatt ccatctggct ctaatgatcc
    21181 ttatgctcta cgtcgtgcaa cgcagggtat cgttcgtatt cttgatcatt ttggatggcg
    21241 catcccaatg gataagctgg tagatagctt atatgacttg tcctttgata gtttgaccta
    21301 taccaataag gcggatgtga tgaactttat ccgtgctcgt gtggataaga tgatgggcaa
    21361 agccgcacca aaagacattc gggaagctat tctagcaagt tcaacctttg tggttccaga
    21421 aatgttagcg gtagctgaag ctcttgtcaa agccagccat acggaaaatt acaaaccagc
    21481 agttgaatct ttgtcacgtg cttttaattt ggcagaaaaa gcggacgctt ctgttcaggt
    21541 ggaccctagc ctctttgaaa atgagcaaga aaacactttg tttgcagcta ttcaaggctt
    21601 aactctagca ggcagtgctg cccagcagtt agaacaggtc tttgccctta gcccagtgat
    21661 taatgatttc tttgataata caatggttat ggcagaagat caagccctta agaacaaccg
    21721 tgtagctatt ttatcagatt tagtaagcaa agctaagacc attgctgctt ttaaccaact
    21781 aaacaccaag taaatagaaa ggaaagagcc gttggggact tagctagaaa agtgagttca
    21841 gtacccgagt ggaaagaaca aggcctttaa acattgcttt tcacagtgag agtcaagtca
    21901 gttttttaag tcttacactt ttttcccagg ctcttagtgg cttaacatgg atcctaaaaa
    21961 aattgctcgt atcaatgagc tggctaaaaa gaaaaaaacg gtaggcttga ctggacctga
    22021 aaaagtggaa caggcaaaac tccgtgaaga atacattgaa ggctatcgtc gctctgtcag
    22081 acaccatatc gaaggcatca aattggtgga cgaagaagga aacgacgtga cccctgaaaa
    22141 attaagacag gtgcaacgtg aaaaaggctt gcacggccgt tcattagatg acccaaaatc
    22201 ataagacaaa aagcagctct gaactgcttt ttgtttatcg tgatgactgt tagcttttat
    22261 tttaaatggt atgatacttc aaagtgacca cacgttttta tattgtatag tcaaaatatt
    22321 gggaaaaacc atgtgtttga gttataatag aagaaatcaa agaaagaagg tagtacttat
    22381 gacaattaaa aaattctctc tactagccat agccagcctc tctttactca gtcttgcagc
    22441 ctgtgatatg gatgataaag acgatcatat ggataatcaa ccaaagacaa gtcaaactag
    22501 caagaaagtt aaactttcag aagacaaagc caaatcaatt gcgttaaaag atgcttctgt
    22561 tacggaagct gatgctcaaa tgctatcagt tacacaagat aatgaagatg gcaaagccgt
    22621 gtatgaaatc gagtttcaaa acaaagatca agaatacagc tataccattg acgccaactc
    22681 aggggatatt gtggaaaaat cttctgaacc aatcaatgac taatcaagaa ctgtaatgaa
    22741 taagacagcc ttaactcttg tgtccaattg tctttgcctc ttaggcgcgg ctgtttgata
    22801 ccgtgttgcc tttgaggctt agttctcttt atgacccaat gatgactctg gattagcaga
    22861 gtgattgttg ggtcttttga ttttctttga taagaaagat tacctcgtta gaaatgagga
    22921 gacgtggttg tcatcagtgg atttttcaag atcatctagt gggataagct acctatctta
    22981 tcagcttttt tttagcttta aaaattcttg ataatatatt tctgataaaa ttagctataa
    23041 tagaatcaaa taggaaaacg cttacttaaa ggagggtaat gaatgtcgca agaaaagtat
    23101 atcatggcta ttgatcaggg aacaacaagt tcacgagcga ttattttcaa tcaaaaaggg
    23161 gaaaaggtta gcagtagcca aaaagaattc cctcagattt tcccacacgc tggttgggta
    23221 gagcacaatg ctaatcagat ttggaattcg gttcagtctg ttattgcggg tgcttttatt
    23281 gagtccagca tcaaaccaag ccaaattgaa gcaatcggaa ttaccaacca acgtgagacc
    23341 acagtggttt gggacaaaaa aacgggtgta cctatttata atgctattgt gtggcaatca
    23401 cgacagacgg caccaattgc tgagcaactc aaacaagatg gccatacaaa aatgatccat
    23461 gaaaagacag gccttgttat tgatgcctac ttttctgcaa ccaagatccg ttggatttta
    23521 gatcatgttc ctggtgctca agaacgagct gaaaaaggcg agttactttt tggaaccatt
    23581 gatacttggc tggtttggaa attgacagat ggtgctgttc atgtgacgga ttattccaat
    23641 gcggctcgta ccatgctcta taatattaaa gacttaacat gggatgatga gattttagag
    23701 cttttaaata ttccaaaaga tatgcttcca gaggttaaat ccaattctga aatttatggc
    23761 aaaacagctg cctttcattt ttatgggggc gaagtgccta tttcaggtat ggcgggtgac
    23821 caacaggcag ctttgtttgg ccaactagct tttgagcctg gtatggtgaa aaatacttat
    23881 ggaaccggtt ctttcatcat catgaatact ggtgatgaga tgcagctatc gagtaataac
    23941 cttttgacca ccattggtta cgggattaat gggaaggttc actatgcttt agaaggctct
    24001 atttttattg caggctctgc cattcaatgg ttgcgagatg gtcttaaaat gattgagacc
    24061 tcaccagagt ctgaacagct ggcactagca tctaccagtg atgatgaagt ctacgttgtg
    24121 cctgccttta ccggtttagg agctccttac tgggattcta atgcgcgtgg atctgtcttt
    24181 ggcttgacac gtggtaccag caaagaagac tttgtcaaag caaccttaca atctatcgcc
    24241 tatcaagtgc gagatgttat tgataccatg caggtggatt caggtattga cattcaacag
    24301 ctccgtgtgg atggcggtgc agccatgaat aatatgctca tgcaatttca ggctgatatt
    24361 ttaggaattg atattgcccg tgctaaaaat ttagaaacaa cagctctagg agcagctttc
    24421 ttagcaggac ttgctgtggg ctactgggaa gacatggatg ctttgaaaga gttaaatgcc
    24481 acaggccagc tctttaaggc tagcatgaat gaatcgcgca aagaaaaatt atacaagggt
    24541 tggaagcgcg ctgttaaagc aactcaagtt ttcacccaag aagaagatgc agatgacgat
    24601 gcaaaataac tagaggagga tggaatggaa ttttcaagag aaacaagacg cttagctctc
    24661 caaaaaatgc aagaaagaga tttagaccta ctgattattg ggggaggtat tacgggtgct
    24721 ggtgtggcac ttcaggcggc agctagtggc ctagatacgg gtctgattga gatgcaagat
    24781 tttgcgcaag gaacctctag ccgttcaacc aaattggttc acggggggct tcgttacttg
    24841 aaacaatttg atgtggaggt ggtttcagat acggtgtcag agcgggctgt ggtgcaacaa
    24901 attgcacccc acattccaaa accagaccct atgttattac ctgtttatga cgaacctggt
    24961 agtaccttta gcatgttccg tttgaaggtc gctatggatt tgtatgatct tttagcaggc
    25021 gtgtccaata cgccagcggc caacaaggtg ttaaccaaag aagaagtctt aaaacgagaa
    25081 ccagacttaa aacaagaagg cttgcttggt ggtggggttt accttgattt ccgtaataat
    25141 gacgcaaggc ttgttattga aaatatcaaa cgagctaatc gtgatggggc tcttattgct
    25201 agtcatgtga aagcagaaga tttcttgcta gatgacaatg gtaagattat tggtgtgaag
    25261 gcgcgtgatt tgctgtcaga tcaagaaatc attatcaagg ctaaattagt catcaacacc
    25321 acaggtccat ggagtgatga gactcgtcaa ttctctcata agggacaacc gattcatcaa
    25381 atgcgcccta caaaaggggt gcatctggta gtggaccgtc aaaaattacc agtgtctcaa
    25441 cctgtctatg ttgacacagg gttaaatgat ggtcgtatgg tctttgtctt gccacgtgaa
    25501 gaaaaaactt attttggaac aacggacacg gactacactg gagacttgga gcacccacaa
    25561 gttactcaag aagatgtgga ttatttgtta ggcgttgtca ataaccgctt tccaaatgcc
    25621 aacgtgacca ttgatgatat tgaaagcagt tgggctggtc ttcgcccctt gttatcaggt
    25681 aatagtgctt ctgactacaa tggtggcaac agcggtaaag tcagtgatga tagttttgat
    25741 cacttggttg atactgtcaa agcctatatt aaccacgaag atagccgaga agctgttgaa
    25801 aaagctatta agcaggttga aaccagcaca tctgaaaaag aattggatcc gtctgcagtg
    25861 tcacgaggtt caagttttga ccgtgatgag aatggactct ttaccttggc aggtggtaag
    25921 attaccgact atcgcaaaat ggctgaagga gcattgacag ggattattca aatcctcaaa
    25981 gaagagtttg gcaaatcctt caagcttatc aattcaaaaa cctatcctgt ttcaggaggt
    26041 gaaatcaatc cagccaatgt agattcagaa atagaagctt atgctcaatt aggaacactt
    26101 agtggtcttt cgatggatga tgctaggtat ttggcaaacc tttatggttc taatgcgcca
    26161 aaagtctttg ccttaactcg tcaattaaca gcagctgaag ggttaagttt agctgaaacc
    26221 ttgtccttac attatgcgat ggattatgaa atggctctta aaccgacaga ttatttcttg
    26281 agaagaacaa atcacctctt atttatgcga gatagcctag atgctttgat tgacccagtg
    26341 attaatgaaa tggctaaaca ttttgaatgg tctgatcagg aaagagtggc acaagaagac
    26401 gatcttcgtc gcgtgattgc agacaatgat ttgagtgccc taaaaggcca gcaggagggt
    26461 taatatggat atctttggtg aatttttggg tacagcctta ttggttttac taggaaatgg
    26521 tgtggtagca ggggttgtct tacccaaaac gaaaacccat gcttctggct ggatcgtgat
    26581 tgcgactggc tggggaattg cagttgctgt ggcagttttt atcagtggca aagttgcccc
    26641 tgcccacctc aatccagctg ttagccttgc ttttgccatg agtggaacca ttgcttggtc
    26701 aacagctatt gcctatagcc ttgcccaact attgggagct atggttggtt caactctggt
    26761 attccttcag ttcaggccac attatctggc tgctgagagt caggctgata ttttagggac
    26821 atttgcgaca ggtcctgcta ttcgagatac cagctcaaac ctattgagtg aaatctttgg
    26881 gacctttgtc ttgatgcttg gtattttggc atttggctta tatgatatgc cagcaggact
    26941 aggaaccctc tgtgtaggta ccttggttat tgggattggg ttatccttag gaggaacgac
    27001 aggttacgcc attaacccag cgcgtgattt aggacctcgt ctagttcatg ctattctacc
    27061 actcaacaac aagggagatt ctgattggtc ttatgcttgg atacctgttg taggaccaat
    27121 tattggagcg gccttagcag ttttgctttt ccaagtcatg tcctaaccat cactaaaggg
    27181 gttggctatc ataggccagc tcctttgctt tttctttata ggattaggta aaacgctttc
    27241 atatcttaaa aacaactgct ataataatac tattatgaca ttaacgacaa agaccataca
    27301 tgttatcggg gcatcctttg ctggtctagc ctttgttgat aaatataagg acctcaatcc
    27361 agattcccaa atcattctca ttgataagga aagctgtccc aattatattc caaatggtat
    27421 caaccaatta ttcagaggag acattcaaga cctgtctgat gctatgtggg gaagggcctg
    27481 cctagcagca caaatagaat ctaaccaccg gtttattcaa gcagaagtgt tagctatcga
    27541 agctccctca aacaccttgc ttctcaaaga cagtcaagga cgagtttttg aagaaggtta
    27601 tgaaacgctt gtttgtgcca tgggtgctag cccccagtct cattatattg agacgtcaca
    27661 gaccaacaaa gttttggtaa ccaaatatta tgaagaaagc caagctagtt taaaactgat
    27721 tgaggctagc caagaggtct tggttattgg tgccggcttg attggcttag accttgccta
    27781 cagtttgtcc ttacaaggca aacgggtcaa actgattgaa gcagccgaac gtccagattt
    27841 ctatcagact gacgcagaac tgatagcccc tgtaatggca gaaatgtcca ctcaccatgt
    27901 cacctttatt aataataagc gtgttacggc tatccatgag atagagggaa aagttgtcgc
    27961 tcataccgag caaggggaca ccttccaagg tgatttggct attttagcca ttaactttag
    28021 gccaaataca caccttttgc aaggacaagt ggcttgtgct ctggataaga ccattttggt
    28081 caatgaaaac ttacagacca gccaggctaa tatctatgcc attggagaca tggtttcatt
    28141 gtattttggt atattgggaa tggattacta tacacctttg attaaccaag caatgaagac
    28201 aggtcaggct ctggctctgc atttagctgg atatcctatt cctccattac agacggtcaa
    28261 ggtattagga agttctcact ttggttatta tcgcgcgagt gttggtgtga cagaagaaga
    28321 agcagagctt tatatggata cgtgtagcta tttgtaccaa aatggggatt ctaaaaacct
    28381 cttttggctg aaattaatag ctagaaaaac ggatgggatt ttaataggcg ctcagctcct
    28441 atccaaaacc aatgctcttg tcatcgccaa tcaattaggt caggctctgg ctctgaaagt
    28501 tacagacggt gacttagcat ttcaagattt tctcttttta caaggtcaca gtgatttggc
    28561 ttatcatctt catgaagctt gtctcaaatt atttgaaaag aggctccgcc atgaagattg
    28621 aagacttaat ggataaagaa cgaaaagctc aatatcgtct cttggtgacc ctttatcatg
    28681 ctaaagaaac gttaaggcta aaagacctaa tgaggctatc aaacttgtct aaagtgaccc
    28741 tattaaaata cattgacaac ctaaatcatt tgtgtcgaga acaaggttta gcctgtcaat
    28801 tacttttaga aaaagacagt ctgagcttaa aagaaaatgg ccagttccat tgggaggatt
    28861 tggtagctct gcttctcaaa gaatcggttg cctatcagat attgacctac atgtactgcc
    28921 atgaacactt taacatcacc aacttatccg ttgaactgat ggttagtgaa gctactctaa
    28981 acagacaact agctcacctc aaccagcttt tatcagaatt tgatctggcg ctctcgcaag
    29041 gcagacaact gggaagtgag cttcagtggc gttatttcta tttggaattg ttccgtcata
    29101 ccttgaccag acaaggcata gacgccttgg tcaatcagtt agacgcttcg catttggcta
    29161 ccttaattga gcggctgatt ggccagtcat tatcggcaga agccttagaa caattattga
    29221 tttggttggc gatttcgcaa gcgcgaatgt cctttcaaaa aggctacaat gatcattttt
    29281 taagagatag tgactttatg acatctaata ttttctttaa gcgcttagaa agcatgcttt
    29341 tacattactt aagacgctat gcccttgaat ttgatgcttt tgaagctaag agcctctttg
    29401 tctttttgca tgcttatcct ctacttccta ttgccagcat gaagtatagt ttgggttttg
    29461 gaggtcctat tgctgatcac atttcagaag ccttatggct tttaaagaag gctcatgtga
    29521 ttatccatca gacaaaggag gaaatcattt atggcttggg gatatttttt tccaaagctt
    29581 acttttttaa aggagctatc cttagccaac caaccaatag ccaatacctt tatcaattag
    29641 taggcgaaga taaaagagcg cttttaaggg ttatcataaa ccatttagtg ctgcaaatgg
    29701 gtcaggaaac ggatttcagt cagcagttga gtgacgacat tttagcattg ttggtttttt
    29761 ccattgagcg ccaccatgag cctcttttag tagggcttgc cttggggcaa aataaggtgg
    29821 aagcagccat tgcggaattg gccattagac gtcatttggg gcatcgtagg gattttcaat
    29881 taatgcccta tgatcatcaa aaagtttatg actgtctgat tacctatcag actgtctatt
    29941 taccgagaca agatttaccc tattaccggt taaaacagta tagctccccc tatgaactca
    30001 ctgccttaga agcttttctc aaagaccttt tccagcaaaa aaatacacga gaagaggaac
    30061 tctcactcag cccagcagct aagtctagtt ttgcacacaa aactgtgtga tacttggtct
    30121 tttctaagga ctgttacact tttataataa taaagtagga tagtctaaag gtagatgatt
    30181 tgaaaaagct tatcaaatag agcttaacaa aatcgaaaat ttttggaggt aagaaatgaa
    30241 atttttccta gatacagcta atgtagccgc tattaaagcg attaatgaac ttggcgttgt
    30301 agatggtgtc accaccaatc caaccattat ctcacgtgaa ggacgtgatt ttgaaacagt
    30361 tatcaaagaa atctgtgaca tcgtcgatgg cccaatcagt gctgaagtga cagggttaac
    30421 agcagatgct atggtagaag aagctagaag cattgctaag tggcatgaca atgtcgtcgt
    30481 taaaattcca atgaccacag aagggttaaa agcaacaaac attctttcaa aagaagggat
    30541 taaaactaat gtgaccttga ttttcacagt aagccaaggt ttaatggcaa tgaaagcagg
    30601 cgcaacttac attagcccat ttatcggtcg tttggaagat attggcacag atgcttatca
    30661 attaatcagc gaccttcgtg aaatcattga cttgtacgat ttccaagcag aaatcatcgc
    30721 agcaagtatt cgtacaacag cccatgtgga agctgttgct aaattaggcg cacacattgc
    30781 gacgattcca gaccccttgt ttgctaagat gacgcaacat cccttaacga caaatggttt
    30841 gaaaaccttt atggaagatt gggcttcctt taaaaaataa ggctaaagag tctgtggcta
    30901 ctgtgtcaac aggctctctt atattcatcg tccaaaagaa ttcaccaatg atgtccaaac
    30961 tagttttttt ctggcaaaat agagaaaaag aatttaggga ctttggagga ttttctgaaa
    31021 aaagcgtcta tttttgtgat acaatagaca atagaaaacg tttaatttta gttgtaataa
    31081 acagagaggt tttacttatg acatttgatg caattgacca gttggcagta aacactgtcc
    31141 gcaccctatc aatggatgcc attcaagcgg caaattctgg acacccagga cttccaatgg
    31201 gggcagcacc tatggcctat gttctttgga atcacttcat gaacatcaat cccaaaacaa
    31261 gccgtaattg gtcaaacaga gaccgtttta tcctatcagc aggtcatgga agtgccatgc
    31321 tttatagctt gttacactta gctggttatg atttatctgt agaagattta aagaacttcc
    31381 gtcaatgggg ttctaaaaca ccaggtcacc cagaagtgaa tcacacagac ggtgtcgaag
    31441 caaccacagg acctcttggt caagggatcg caaatgccgt tggtatggcc atggcagaag
    31501 ctcacctagc agctaaattt aacaaaccag gctttgacat cgttaatcac tacacctttg
    31561 ctttgaatgg tgacggtgac cttatggaag gggtcagcca agaagcagca agtatggcag
    31621 gacatttaaa acttgggaaa ttggtcttgc tatatgattc aaacgacatc tctcttgatg
    31681 gtccaacctc tatggctttc acagaagatg tgaaaggacg tttcgaagct tacggttggc
    31741 aacatatcct tgtgaaagat ggaaatgatt tagaagaaat tgcagctgct attgaagcag
    31801 ctaaagctga aactgagaaa ccaaccatca tcgaagtcaa aaccattatt ggttttggtg
    31861 ccgaaaaaca aggaacttca gctgttcacg gcgctcctct tggagcagaa ggcattgctt
    31921 ttgccaaaaa agcttaccaa tggactcatc aagattttga agtgcctgct gaagtaacag
    31981 agcgctttgc tcaaggtctt caagcgcgtg gtgaaaaagc agaacaagcc tggaatgact
    32041 tgtttgcagt ttatgaagca gaatatccag aattagcagc agaataccaa aaagccttta
    32101 ctaacgaagc tgctcaagta gaacttgaag cgcacgaact tggtagttca atggcgagtc
    32161 gtgtatccag tcaacaagcc atccaacaaa tctcagaaca agtagcctct ttctggggtg
    32221 gatcagcaga tctttcagca tctaacaaca caatggttaa ggctgaaaca gatttccaac
    32281 caggtcacta cgaaggccgt aacatctggt ttggcgttcg tgaatttgcc atggctgcag
    32341 ccatgaacgg tattgccctt cacggcggga cacgcgttta cggcggaacc ttttttgtct
    32401 tctctaacta tcttcttcca gctgttcgta tggcagcgct ccaaaacttg ccaacagttt
    32461 atgtcatgac acatgattct atcgctgttg gtgaagatgg gccaacgcac gaaccaattg
    32521 aacaattggc aagtgttcgc tcaatgccta actttaacgt tattcgtcca gcagacggta
    32581 acgaaacaaa tgctgcttgg aaacgtgcca ttgctgaaac agatcgccca accatgcttg
    32641 tattgacgcg tcaaaacctt ccagtgcttg agggcacaaa agaattggca gaagatggtc
    32701 ttaacaaagg agcttacatc ttgtcagaag ccaaaggcga ccttgaaggt atcttgattg
    32761 caacaggttc agaagtgaaa ctggcaatgg atactcaaga agctcttgaa gcagaaggca
    32821 ttcatgtgcg tgtcgtatct atgccatcac aaaatatctt tgacgaacag tcagcagagt
    32881 ataaagaaag catcctacca gcagctgtga ctaaacgtct cgctatcgaa gcaggttcaa
    32941 gctttggctg ggcaaaatat gttggcctag caggaaaaac attgaccatt gacacttggg
    33001 gtgcttcagc tccaggaaat cgtatcttcg aagaatatgg tttcactgtg gcaaatgcaa
    33061 ctgagttata caaatcactc taggttacac atcccaaaca gagtaaatca aatgatttac
    33121 tctgtttttt taaaataaaa taaccagttc tttcttttcc tcaaactgcc taatacactt
    33181 cttttgataa gaagtcattg acaaaacgat tcataaaaat tataatttaa ttgttattgt
    33241 attagcgtaa aagtacaaaa aataaaggag tataaatgga aaagaaacga cagcgcttat
    33301 acgatgtgat tagacaggct tatgattatc cagaaaaccg tgaaaatgtg gccttgtcgc
    33361 agctactttt agcagcttct aatagattaa ttaaacacag taacccactt cttgttgcct
    33421 accaactaaa ccaagatgtg gataattact tacttgataa tgacatttta cttccaaaat
    33481 cgctttgccg ttttaagcag tcattggaaa agtatatttt agaggaagcc tcatcagtct
    33541 agggagaaaa agagaagaaa aggaaaaagg ataccatgat agaatttaaa catgtttcaa
    33601 agttgtatgg cgacaaagaa gctctcagcg atttaaacgt caccatcaat gacggtgaaa
    33661 tttttggcct tatcggacat aatggtgcag gtaaaaccac gaccatcagc attttgactt
    33721 ctattatcga agcgagttat ggcgaagtat ttgtggatgg tcaacttctg acagaaaata
    33781 gagaagctat caaaaaacaa attgcttatg tgccagattc tcctgatatt ttcttgaatc
    33841 taactccaaa tgagtattgg cagtttttag ctaaaattta tggggtgtca gatgaggacc
    33901 gagaagaacg gttagcacag ctgacaacac tttttgaact gaaggaagaa gttaatcaaa
    33961 ccattgatag tttctcacat gggatgcgcc aaaaagtgat tgtgattggt gctcttgtat
    34021 caaatcctaa catctggatt ttggatgaac cattaacagg tttggatcct caggcttctt
    34081 ttgaccttaa ggaaatgatg aaagctcatg cagcttctgg acatacggtc ttattttcga
    34141 ctcacgtgct atcagtcgct gagcagttat gtgatcgtat cgggattcta aaaaaaggga
    34201 aattgatttt tgttggaacg attgatgagt tgaaagaaca ccatcctgac aaagacttgg
    34261 agagcatcta ccttgaactc gcaggtcgta aggcgcaaga agaggggtga tgggatgaat
    34321 tggtctacta tttgggaact tatcaagata aacattcttt attccaatcc acaaagttta
    34381 gctaacctca aaaagcgtca ggaaaaccac ccgaaagaaa acttcaaagc ctataaaagc
    34441 atgatgaggc aacaggcctt aatgattgcc atgtttttgg tgatttacct ttttatgttt
    34501 attggtgtcg atttcagtca ttatccagga cttttctcct ttgacgttgc tatgtttttt
    34561 atcatgtcaa ccttgacagc ctttagctct ctttacacca ttttttatga aagtaatgac
    34621 ttaaaacttt atattcactt accagtaacg tcagaagaac tctatattgc aaaaattgtc
    34681 tcgtcgctag ggatgggtgc tgtctttttg atgcctctta tctctcttct cttaattgcc
    34741 tattggcaac tattgggaaa tcctttgtct attttagtag ctattgtgct ctttttagtg
    34801 ttactagtga gttcgatggt attagctatt tatatcaatg cttgggtggg caaaatcatt
    34861 gtaaggagtc gcaagcggaa actcatttca actatcatga tgtttgtctc aacttttgga
    34921 gctttcgtct taatttttgc gattaatatt agtaacaaca agcgtacgat gacggatggc
    34981 gtatttactg attacccaac tattccgtat ttcaaaggat tctatgatgt tattcaggca
    35041 ccgttttcta ctgcggccct ccttaacttt tggttgccat tgctactgat tttagctatg
    35101 gtatatggta ttgtcacaaa agtgatgcca acttattatc gtgaggcttt ttatattagt
    35161 aacgagaaca aagccaagca aactaaaaaa ccagttaatc gtcctcatca gaatcaatca
    35221 ctgacgcagt tgttgcgaaa acatcatctt ttaacgttac aaaatgcaac tttactgaca
    35281 caaacctatc tcatgcccct gatgtatgtg atgcttttta tcggtccaag tttgtcacgt
    35341 ggcacaggtt tctttaagca tatttcccca gattactttg gggtggcctt attatttggg
    35401 gtcagtttgg gtgtcatgtg tgcaacacca accagcttta ttggagtagg tatttcactt
    35461 gaaaaggata actttacctt tattaaaagc ttaccaataa cgttaaaaaa attcttgctg
    35521 gataaatttt gcttgctcgt cggtctgcaa ctgattgtgc ctatggtgat ttatcttgtg
    35581 tttggtctct ttgtgttgca cctgcatcct cttctaacga ttgctttttg tcttggctac
    35641 gccctgtctc tgattgtgca aggggaattg atgtatcgtc gtgactaccg gcttctggat
    35701 ttaaaatggc aagacatgac acaactcttt accagaggaa atgggcagtg gctaacaatg
    35761 ggccttatct ttggtaattt aatagtcaca ggtgtagtag gttttggagc tgttatcatt
    35821 gccaatatta tccaacaacc tctgttgatt agtattctat tgagttgtct gacactaatg
    35881 gttttaggga ttgctcactt atggattcaa aaaaccttct ggaaaagctt agagaagctt
    35941 taacgaagag agatcattat tcggaaaaac agtcctctgg ggctgttttt atgctataat
    36001 ttgggtatga tgaaacgaca atttgaagat gtaacacgta tcgtaattaa aattggcact
    36061 agttcattag tgctaccaac aggtaaaatc aatttagaaa aaatcgacca actggctttt
    36121 gtgatttcaa gtttgatgaa taagggcaag gaagttattt tagtatcatc gggagcgatg
    36181 ggatttggac tagatatttt gaaaatggaa aaacgtccga ccaatcttgc taaacaacag
    36241 gcagtttcca gtgttggtca ggtggccatg atgagtcttt attcacaaat ttttgcacac
    36301 taccaaacca acgtgtcaca aattttattg acgagagatg tggtggtttt cccagaaagt
    36361 ctagccaatg ttaccaatgc ttttgaaagt ctgattagtt tgggcattgt gccgattgtt
    36421 aatgaaaacg atgcggttag tgtggatgaa atggatcatg cgactaaatt tggagataat
    36481 gaccgtttat ctgctgttgt tgctggtatt acaaaagctg atttattaat catgttgtct
    36541 gatattgatg gcctttttga taaaaaccct actatttatg aggatgcgca gttacgcagt
    36601 catgttgcag tcattacgca agaaatcatt gcgtcagcag gtggtgcggg cagtaaattt
    36661 ggcacaggtg ggatgcttag caaaattcag tctgctcaga tggtatttga aaacaaagga
    36721 cagatggtct tgatgaatgg agctaatccc agagatattt taagagtgct tgaagggcaa
    36781 ccactaggca cctggttcaa gcagatagaa gaggtaacac atgactgata tgagaaggtt
    36841 agggcaaagg gcaaaacaag ctagtctttt aatagcacct ttgtcgacgc aaattaaaaa
    36901 tcgcttttta agcaccttag caaaggcatt agttgacgat acacagactc tcttagccgc
    36961 taaccaaaaa gatttagcga atgctaaaga acatggtatt tctgacatca tgatggatcg
    37021 cttgcgctta acttcagaac ggattaaagc gatagcacag ggagttcaac aggttgctga
    37081 cttagctgat ccaatcggac aagtcattaa gggctatact aacctcgatg gccttaaaat
    37141 cctccaaaag agagttcctc taggggtgat tgcgatgatt tttgagagtc gcccgaatgt
    37201 ttcagtggat gcttttagtc tggcgttcaa aaccaataat gccatcatcc taaggggagg
    37261 aaaagatgcc cttcactcaa ataaagcttt ggtgaagttg atccgacaaa gtctggaaaa
    37321 atcagggatc accccagatg ctgtccagct agttgaggat ccctcacatg ctgttgctga
    37381 agaattgatg caggcaacgg actatgtgga tgtgttgatt ccacgtggtg gtgctaaatt
    37441 aattcagacc gttaaggaaa aagcaaaagt gcctgtcatc gaaacaggtg tggggaatgt
    37501 gcatatctat gtggatgctc aagctgactt agatatggct actaatattg ttatcaacgc
    37561 taaaacaaag cgcccaagtg tttgcaatgc agctgaggga ttggtaattc acgaagctgt
    37621 agcagcaagg tttattccta tgcttgaaaa agcgatcaat caggttcagc cagtagaatg
    37681 gcgggcagat gacaaggctt tgccgttatt tgagcaggca gtgcctgcaa aagcagagga
    37741 ttttgagacc gaatttttag attatatcat gtctgttaag gttgtttcct ctttggaaga
    37801 agctatttct tggataaatc agcatactag tcaccattcc gaagctatca tcacaaggga
    37861 tattaaggca gcagagacct ttcaagattt agtggatgct gctgctgttt atgtcaatgc
    37921 ttcaacacga tttaccgatg gttttgtttt tgggcttggt gctgaaattg ggatttctac
    37981 tcaaaaaatg cacgcccgtg ggccaatggg cttagaagcc ttgaccagca ctaaatttta
    38041 tattaatggg gatggacata tcagagaata agctactaga aggtccctta atgactaatg
    38101 aaagagaaaa agaagaagca aaggttgctt tagaagaact tttacatcag attcaggtga
    38161 ttcatttgga attgaaaatt gtccgaaatt ggggctattt tgatcttttt ggagacggta
    38221 tgcttgtcag ctggatcaaa cgtcgaaaaa tgagcaaggt caaccaatta ttaatagact
    38281 tacaagttac tcttcaaaac tgccgaagat agctggcaga cgtcgatagg ctattattag
    38341 ctgacttacc gaatatgagt tgggataaaa tctttgatat ttggttagac aacctgctga
    38401 cagattgttg tgtgattaaa gaagctaaaa attttttaca agtagttgaa attctagagg
    38461 cagcggctaa gtcagtgctt gctaagttgg ctgatagcga acaaaaagaa caagattaga
    38521 gaagaggaag attcccacga gaatgaaatc cttttctctt actttttcat ttctaaacct
    38581 tttgaagtat ggtacaatta aagttatgac aaaagaattt catcacgtga ccgtactcct
    38641 tcacgaaaca gtggacatgc ttgacataaa gcctgatggg atttatgttg atgcgacgct
    38701 aggtggctca ggccactcag cttatttgtt gtccaaactt ggtgaagaag ggcacctcta
    38761 ttgttttgac caagaccaaa aggctattga caatgcacaa gttaccctca aatcttatat
    38821 tgacaaagga caggtaactt ttattaaaga taattttaga cacctcaaag cacgtttaac
    38881 agcgcttgga gttgatgaaa ttgatggtat cttatacgac cttggtgttt ccagcccgca
    38941 attggatgaa agagaacgag ggttttctta taaacaagat gctccattgg atatgcgcat
    39001 ggatcgtcag tcgctcttaa cagcttacga agtggtgaat acctatccat tcaatgattt
    39061 ggttaagatt ttcttcaaat atggtgaaga taaattctcc aagcagattg ctcgaaaaat
    39121 tgaacaagca agagctatta agcctattga gacaacaaca gagttggcag aattgattaa
    39181 ggcagcaaag ccagctaaag agttgaagaa aaaaggccac cctgctaaac agatttttca
    39241 agctattcgc attgaagtca atgatgaatt gggagcggcc gatgaatcta ttcaggacgc
    39301 tatggaatta ttagcccttg atggtcgtat ctcagtcatt accttccatt ctctggaaga
    39361 tcgcctaacc aagcagttgt ttaaagaagc tagtacggtg gatgtgccaa aagggcttcc
    39421 tctaattcct gaagatatga aacctaagtt tgaacttgtt tcacgtaagc cgatcttacc
    39481 tagtcattca gagttaacag ctaataaaag ggcacactca gccaagctac gtgttgccaa
    39541 aaaaattcgg aaataagcct atgacaaatg aaaaaagaac tcaggcagtt acaaatgcct
    39601 tgcaaaaacg aataaaaact ttctctagaa ttgagaaggc tttttatact gctatcattg
    39661 taacagcgat tacgatggca gtaagtatta tttacctgca aagtcgtaag ttgcagttac
    39721 aacaagaaat cactagttta aatagtcata tttcggatca aaaactagag cttaataatg
    39781 ccaagcaaga agtaaatgag ttatctcgcc gtgatcgtat tatagatatt gctggtaaag
    39841 caggcttaag caaccgtaat aataatatca agaaagtcga gtaatctatg aaaaaatggc
    39901 aaaaatatgt tttagattat gttgtgcgcg ataggagaac tccagtcgaa aatcgcgttc
    39961 gagttggaca aaatatgatg ctcttaacta tctttatttt ctttattttc attattaatt
    40021 tcatgattat tattggaaca gatcaaaagt ttggagttag tttgtcagaa ggggcgaaga
    40081 aggtttatca agaaaccgtt acgatccaag ctaagcgtgg gaccatttat gatcgaaatg
    40141 gtacagctat tgcagtggat tctacgactt atagcatata cgcaattttg gataaatcat
    40201 ttgtctcggc ttcagatgaa aagttatatg tacaaccttc tcagtatgaa acagtagctg
    40261 atattttaaa aaagcatctg ggaatgaaaa aaacagatgt aattaaacag cttaagcgta
    40321 aaggactttt ccaagtctcg tttggaccgt cagggtctgg catttcatat agtactatgt
    40381 ctactattca aaaggctatg gaagatgcca aaatcaaggg aattgctttc acaactagtc
    40441 ctggtcgtat gtatccaaac gggacatttg cttcagaatt tataggcttg gcatctctaa
    40501 cagaagataa aaagacaggt gttaagagtt tagttggaaa aacaggtcta gaagcttctt
    40561 ttgataaaat tttatcaggt caagatggtg ttattactta tcaaaaagat cgaaatggga
    40621 ccacactctt gggtacaggt aagactgtca agaaagctat tgatggcaaa gatatttaca
    40681 caacgctatc tgagcctatc cagaccttct tagaaaccca gatggatgtt tttcaagcca
    40741 aatcaaatgg tcagttggcc agtgcaacac ttgttaatgc taaaactggt gaaattttgg
    40801 caacaacaca acgccctact tataatgctg atactctgaa aggacttgaa aatacgaact
    40861 acaaatggta cagtgcactt catcaaggaa attttgagcc aggctcaacc atgaaagtga
    40921 tgactctggc agcggctatt gatgataaag ttttcaaccc aaacgaaacc tttagcaatg
    40981 ctaatggttt aacaattgca gatgctacta ttcaagactg gtctattaat gaaggcattt
    41041 ccacaggaca gtacatgaat tatgcacaag gatttgcctt ctcaagtaac gttgggatga
    41101 ctaaacttga acaaaaaatg ggtaatgcaa aatggatgaa ttatttgacg aagttccgct
    41161 ttggttttcc tactcgtttt ggtttaaaag atgaagacgc aggtatattt ccttctgata
    41221 atatcgtgac tcaagctatg agcgcttttg gtcaagggat ttctgtaacc cagattcaaa
    41281 tgcttagagc ctttactgct atttctaata atggtgagat gttagagcca caatttatca
    41341 gtcaaattta tgatcctaac acagcaagtt ttagaacggc aaataaagaa attgttggaa
    41401 aacctgtatc aaaaaaagcc gctagtgaaa caagacaata catgattggt gtaggaacag
    41461 accctgagtt tggaacactc tattcaaaaa catttggacc aattattaaa gtgggtgatt
    41521 tacctgttgc tgttaaatca ggaacagcac aaattggttc agaagatgga agtggttatc
    41581 aagatggtgg attgactaac tatgtctatt cggttgtggc aatggtgcca gctgataaac
    41641 cagacttttt gatgtatgtt actatgacta aaccacaaca ttttggtccc cttttttggc
    41701 aagatgtggt taacccagta ttggaagaag catacttaat gcaagataca ctaactaagc
    41761 cagtagtatc agatgctaat cgtcaaacaa cttataaatt accaaacttt gtaggaaaga
    41821 atcctggtga gacatcaagc gagttgcgtc gaaatcttgt ccagccagtt gtccttggta
    41881 ctggcagcaa gatcaaaaaa gtatcgcatc agcccggtca aacgttaaca gaaaaccaac
    41941 aagttctcat attatcagac cgttttgtgg aggtaccaga catgtatggc tggacaaaat
    42001 ccaatgttaa aacctttgct aaatggactg gaatagacat cagctttaaa ggaacagatt
    42061 ctggtcgtgt tatgaaacaa agtgttgatg ttggtaagtc cttgaaaaaa ataaaaaaaa
    42121 tgaccattac tttaggagat taacatgttt ttaaccctta ttgcagcaat catatccttt
    42181 atggtatctg ctttcacaat gccttatttt atcaaattct atcaattgaa aaaaattggt
    42241 gggcaacaaa tgcatgaaga tgtcaagcaa catttagcta aagctggaac accgactatg
    42301 gggggaactg tttttctttt agtagctacg gcagtatcgt tattggttag tctattttcg
    42361 atcaaaaaca ctcaaagttt agctttgatt tcaggcattt tatcaattgt tgtcatttac
    42421 gggattatcg gatttttgga tgatttttta aaaattttta aacaaatcaa tgaaggattg
    42481 acagccaaac aaaagttagc cttgcagttg gttggaggat tgatgttcta ttttttacat
    42541 gttagtccaa gtggcatttc ctctattaat gtgtttggtt atcagctgcc gctgggaatt
    42601 ttctacctat tctttgtttt gttttgggtg gttggttttt caaatgccgt taatttgaca
    42661 gatggtatcg atggcttagc ttctatctca gtggtgatta gtctagtgac ttacggcgtt
    42721 attgcctacg ttcagagtca atttgatgtt ttgttactga ttggagcaat gattggagcc
    42781 ttgcttggct tcttctgctt taatcacaaa cctgctaaag tatttatggg agatgtaggt
    42841 agtttagccc ttggagctat gttggctgcc atttctattg cgcttcgtca agaatggact
    42901 ctcctgatta ttgggatcgt ttatgttctt gaaacaagtt ctgtgatgtt gcaagtgtcc
    42961 tatttcaagt acaccaagaa aaaatatgga gaaggtcgtc gtatttttag aatgacgcct
    43021 ttccaccacc atttggaatt gggaggcttg tctggtaaag gcaagaagtg gtcagaatgg
    43081 caggtagatg cttttttgtg gggagtagga agtctagcta gtttactagt tttagctatc
    43141 ttatacgtgt tctaaggggg attatcaaga atagtcacca atagcatttt gattaacatc
    43201 atcaataaga gagtggagat gacccacaaa tgctgatttt gtgagtgttg tcccactcct
    43261 tttgcttgtc tctaacaata atgatataat atggaagaaa acgaggtaaa acatgtcatt
    43321 taaagattat cattttaagc aatacgttca gcaagcgctg gaagaaattg gctttgtcaa
    43381 tccaacagag gtccagaagc ggctgattcc tattgttaac tccggtcgtg acttagttgg
    43441 ggaatcaaaa acaggttcag ggaaaaccca cacctttttg ttgcccattt ttgaaaaact
    43501 tgatgaagct aaagcagaag tgcaagtcgt tattacagca cctagccgtg aattagcaac
    43561 tcaaattttt gatgcttgta aacagattgc taagcacttc caagaagaga ttcgtttggc
    43621 caactatgtt ggaggcacag acaaactccg tcagattgaa aaactaaaag gcagccagcc
    43681 gcatattgtc atcggaaccc caggtcgtat ttatgacttg gtcaagtctg gtgatttagc
    43741 gattcataaa gcaacgacct ttgttgtgga tgaggccgat atgaccatgg acatgggctt
    43801 tttggatacg gtagataaga ttgcagcttc gcttccaaaa tctgttcaga ttttggtgtt
    43861 ctcagcaact attccacaga aattacaacc ctttttgaaa aaatacttga ccaatcctgt
    43921 tattgaacaa atcaagacaa agaccgttat cgcagatacc attgataact ggcttgtgtc
    43981 gacaaaaggg cgtgataaga atggtcaact gttagagatt ctcaaaacca tgcagcctta
    44041 tatggctatg ctttttgtca ataccaagga acgcgcagat gatttgcatg cctttttaac
    44101 agctaatggc ttaaaagtgg ctaagattca tgggggcatt cctccaagag aacgtaagcg
    44161 catcatgaac caagtcaaaa aactggactt tgaatacatt gttgccacag acttagcagc
    44221 gcgtgggatt gacattgaag gggtcagcca tgtgatcaat gacgccatcc cacaagactt
    44281 gtctttcttt gtccatcgtg taggccgaac aggtcgaaat ggtatggcag gaactgccat
    44341 tacactttac cagccaagtg acgactcaga tatcaaagag ctggaaaaaa tggggattgc
    44401 ctttacaccg aaggtgctca aaaatggaga atttcaagat acctatgacc gtgatcgccg
    44461 tcaaaaccgt gaaaaagcct atcaaaaatt agacacagaa atgattggtt tggtcaaaaa
    44521 gaaaaagaaa aaagtcaaac caggttacaa gaaaaaaatt cagtgggcgg ttgatgaaaa
    44581 acgtcgcaaa gaacgtcgtg ctgaaaatcg tgctaagggc cgcgctgagc gtaacgccaa
    44641 aaaacaacat ttttaagaac aaagagcaag caagtgatca attggcatct aatcttgctt
    44701 tttttacacc tgctacttgt attgtttacc atttgttaac acaaaaagtg atcatagctt
    44761 ttgcttattg ctataatacg gatttactat tttacatgtg ggtctaagag tgttagaata
    44821 ttaagacaaa caaatacaaa ggggaaatac atgacatcag tgtttttaac cagtggttgg
    44881 gctttttatg attacctcat ctcaccgatt ccacatggaa aactttttag ttggcatgct
    44941 gtttttgatg ccattccaaa tattatccaa cggcttccaa ttacgcttgg tttgacacta
    45001 tcaggagcaa cctttggctt ggttttggct ttgatttttg cccttgtcaa aattaataaa
    45061 gtgaagctac tataccctat tcaagctatt tttgtgagtt tcttgcgagg aactcctatt
    45121 ttggtacagt tgatgttgac ctactacggt attcccctct ttctaaaatt tctcaatcag
    45181 aagtatggct ttgattggaa tgttaatgca attccggctt ctatttttgc cattacagcc
    45241 tttgctttta atgaagcagc ctacgctagt gaaactattc gggcagccat tttatcagta
    45301 gatacaggtg aaattgaagc agcaaaaagt ctaggcatga cttctgtgca ggtttaccgt
    45361 cgtgtcatta ttcctaatgc aactgttgtg gctattccaa ccttgattaa tgggttaatt
    45421 ggcttaacta aaggaacatc gcttgccttt aatgcaggga ttgtcgaaat gtttgcccag
    45481 gctcaaattc taggcggatc agattaccgt tattttgaac gttatatctc tgttgctctt
    45541 gtctattggt ctatcagtat tttgatggag caagtaggtc gcttgattga aaacaagatg
    45601 gccatcaaag caccagaaca agctagaaat gaaaagttag gagaattgcg ttgatgatta
    45661 caattagaaa tttgagcaag acattctcag ggcaaaaagt tttagatagt ttggctcttg
    45721 atattgaaaa aggtcaagtg attgctttag ttggtgcttc aggagcaggg aaatcgacct
    45781 tccttcgtag cctaaactat ttggaaaaac cagattcagg atccattagc attggtgact
    45841 ttacggttga ttttgagacc attaccacag agcaagtcct tattttacgt cggaaacttg
    45901 ccatggtttt tcaacaattt aatctttttg agcgtcgtac agcccttgag aatgtcaaag
    45961 aaggtcttaa ggtggtcaaa aaactgtctg accaagaagc gactaagctg gcgcaagcag
    46021 aattggctaa ggttggttta gcagatcgaa aacatcatta ctcacgtcat ttatcagggg
    46081 gacaaaaaca acgggtagct ttagcaagag ctctggctat gaaaccagat gttttattac
    46141 tagatgagcc gacctcggct cttgacccgg aattagttgg cgaagtggaa aagtctatta
    46201 ctgatgctgc taaatccgga cagaccatgg ttttagtgag tcatgacatg aattttgttt
    46261 accaagtggc agatagagta ctctttttag atcaagggaa aatcttggaa caaggaacac
    46321 ctgaagaagt ctttagacat cctcaaaaag aacgaaccaa agaatttttt gcaagctatt
    46381 caaaaacata catttaatga tattcttttg ttataataaa aaggtctagg tattcctggg
    46441 cttttcttgt tatgtttttt agaaaaactt atgggaaaga aataatgact aggtggaaat
    46501 atgttagtag aaatctttag cctttatgta aaaggtctca ttctctcaag ccttactatg
    46561 attttaattt gtttaatatg gactttatgg cgagcaaaaa ccaaaagaga taaaacattg
    46621 attgaaagac aagcttttct ttatgagatg ctaatggttg ctattttaac catcccaatt
    46681 ttatcgtttg cttttatgag tattttggtt gtcataaaat cctaatcatt gcaagaaggg
    46741 attatcattg ctaaaatatt ggcaagagta gattaaagga gaaaaatcat gtacgataca
    46801 ttaattattg gttcaggacc agcaggaatg actgccgctt tatacgctgc tagaagtaat
    46861 ttatctgttg ccattattga acaaggggca cctggtggtc agatgaacaa cacttttgat
    46921 atagagaatt acccgggata tgatcatata tctggaccag aattagctat gaaaatgtac
    46981 gaacctttag agaagtttaa cgttgaaaac atttatggta ttgttcaaaa aatagaaaat
    47041 tttggtgact ataagtgtgt gttgactgaa gatgctagtt atgaagctaa aacggttatt
    47101 attgcaacgg gagctaaata ccgtgtacta ggtgttccag gtgaagaata ctatactagt
    47161 cgaggagttt cttattgtgc agtatgtgat ggtgcgtttt tccgtgacca ggatcttttg
    47221 gtcgttggtg gtggtgactc agcagttgaa gaagctattt atcttactca gtttgctaaa
    47281 aaggtaaccg ttgttcatcg ccgtgatcaa ctaagagctc aaaaaatact gcaagatcgt
    47341 gcttttgcca atgataaggt cgactttatc tgggattcag tcgtcaaaga aattcaaggc
    47401 aatgatatta aggtctccaa tgtcctaatc gaaaatgtga aaacaggcca agtcactgac
    47461 cacgcttttg gaggtgtgtt tatctatgtt ggtatgaatc ctgtgacagg tatggtaaag
    47521 gatctagaaa ttaccgatca agaaggttgg attatcacag atgatcatat gaggacaagt
    47581 attcctggta tttttgcgat tggagatgtt cgtcaaaaag accttcgcca aatcacgaca
    47641 gctgttggag atggtgcaat tgcagggcaa ggtgtttatc actaccttga aagtttccct
    47701 tcataaggtt aaaactcctt catttttcct tgtctttccc ttaaaaaagc taaattgcat
    47761 aagaccttta agaaatataa agaacagttt tccttaatat ctttcctaga taaatgttga
    47821 aaattttctg aaaaaaggat ataataaata gtattttagt gaatggagtt tagaatgtct
    47881 ataaaagaac aaacagacaa taacgaacta gaaaatggaa tggtaagggg cttagaaaat
    47941 cgtcatgtgc agttaattgc gattgcagga acgattggaa caggtctctt tttaggggca
    48001 ggacgctcta ttgccctaac aggaccatct attatttttg tgtatatgat tacaggtgcc
    48061 ttcatgttta tgatgatgcg tgccatcggt gaaatgctct attatgatcc agatcaacac
    48121 acctttatca attttatttc caaatacatt ggtccaggct ggggttattt ttcgggtcta
    48181 tcctattgga tttcccttat ttttattgga atggcagaaa tcacagcagt aggtgcttat
    48241 gtgcaatttt ggttcccaag ctggccagcc tggttgattc agttggtttt cttagtctta
    48301 cttagttcga tcaacttaat tgctgtgcgc gtttttgggg aaacagagtt ttggtttgcc
    48361 atgattaaga ttttagctat tttagctttg attgcaacag ctattttcat ggtattgaca
    48421 ggttttgaaa ctcacacagg ccatgctagc ctttccaata tttttgacca tttttccatg
    48481 ttcccaaatg ggaaactaaa gttctttatg gccttccaaa tggttttctt tgcttatcaa
    48541 gctatcgaat ttgtgggaat taccacttct gagacggcta acccaagaaa agttctacca
    48601 aaggctattc aagaaattcc aacccgtatt gtgatctttt atgtgggagc cttggtctct
    48661 attatggcaa ttgtgccatg gcatcagtta ccagttgatg aatctccttt tgtgatggtg
    48721 ttcaaattga ttggtattaa atgggcagca gccttgatta actttgtggt cttgacatca
    48781 gcagcgtcag cgcttaactc aaccctttat tcaaccggtc gtcatctcta tcagattgct
    48841 aatgagactc caaacgcttt gaccaatcgc ttaaagatta atactttatc tcgacaaggg
    48901 gtgccaagtc gtgccattat tgcttcagcg gttgtggttg gtatctcagc tttgattaat
    48961 atcctaccag gagttgcaga tgcgttctca ctcattacgg cctcctcatc aggtgtatat
    49021 attgctatct acgccttaac catgattgct cactggaagt accgtcaatc taaggacttt
    49081 atggcagatg gttatttgat gccaaaatat aaagtgacaa cgcctttgac ccttgctttc
    49141 tttgcttttg tctttatctc tcttttctta caagaatcca cctatatcgg cgccattgga
    49201 gcaacgattt ggattattgt ttttgggatt tatagcaatg tcaaatttaa ataataatta
    49261 gcgatataga actgaatcca ttaactcttt cttatttgag gatgtcatga tggatcagtt
    49321 ctttttttac tttagcgata actcaagtta tttctaaacc ctagccaaag agtccttttt
    49381 aaaatatgtt ataatagtaa caataattca aaggagtccc ctttatgtat aaagatgata
    49441 gtttaactct ccatactgat ttgtatcaga ttaatatgat gcaagtttat tttgagcagg
    49501 gcattcataa tcgtcatgcg gtgtttgaag tctatttccg caaggaacct tttaacaatg
    49561 gttatgctgt ttttgccggt ttacagcgca tggtggagta tctagagcaa tttcaatttt
    49621 cagaaacaga tttggcttac cttgaagaac tgggttatcc aggaaacttt ttgacctacc
    49681 ttaaagagtt acgattagaa ttgaccatcc gttcagctaa agaaggagat ttggtttttg
    49741 ctaatgagcc gattgtgcaa gtagaaggcc ctctaggtca gtgtcagttg gtcgaaacag
    49801 ctcttttaaa cattgttaac ttccagacct tgattgctac aaaggctgct cgtattcgtt
    49861 ctgtgattga ggatgaaccc cttttggaat ttggtacccg tcgtgcacag gaactagatg
    49921 ctgcgatttg gggaacccgc gcagcgatga ttggtggagc agatgcaaca agtaatgtga
    49981 gagcaggtaa acgctttgat atccctgttt caggtacgca tgctcatgct ttagtgcaag
    50041 cctatggtaa tgattatgat gctttcatgg cttatgctaa gacccataaa gattgtgtat
    50101 ttcttgttga cacctatgac acacttaaag taggtgtgcc aacagctatc cgagtagcca
    50161 aagaaatggg tgacaagatt aattttctag gtgttcgatt ggactctggt gacttggctt
    50221 acctctcaaa aacagtccgt cagcagttag acgacgctgg ttttaccgag gctaagatct
    50281 atgcttctaa tgacctcgat gaaaatacca tcctcaactt aaaaatgcaa aaggccaaaa
    50341 ttgatgtttg gggtgttggg accaaactga tcacagccta tgatcagcct gccttaggtg
    50401 ctgtttataa gattgtctct attgagcaag aagatggtag catgcgtgat acgatcaaat
    50461 tatcaaataa tgcagaaaaa gtatccacac caggtaaaaa acaagtttgg cgcatcacca
    50521 gtcgtgaaaa aggcaaatca gaaggcgact atatcacgtt tactgatatt aatgttaatg
    50581 aattaactga gattgagatg ttccatccga cctataccta tattaagaaa accgtcagaa
    50641 attttgatgc aattcctctc ttagttgata tttttgtcaa gggagaatta gtttaccagc
    50701 taccgacctt atctgaaatc aagacttatg ctaaaaaaga atttgacaag ctttgggatg
    50761 aatacaagcg tgtcttaaat cctcaagact acccagtaga cttggcacga gatgtgtggc
    50821 aaaataagat gaccttgatt gataacattc gtaaagatac tcatggaaag agtgaatgac
    50881 atgacattac aagaagagat cattcgacaa ttaggcgtca aggctagcat tgatccacaa
    50941 gaagaaattc gaaaaaccgt tgatttttta aaagcttatt tgcgaaagca ctcattttta
    51001 aaaacttatg tcttaggcat ttctggaggt caggattcta ccctcgcagg aaagctagcc
    51061 cagatggcca ttgcagagtt acgggaggaa acaagtgatc aagcttacca atttatagct
    51121 gttcgcttgc cttacggggt tcaggctgat gaagcagatg cgcaaaaagc cttggctttt
    51181 attgcaccag atcagaccct taccattaat atcaaggcag ctgtggacgg tcaagtagag
    51241 gctctacaag cagcaggcgt tgagatttct gattttaaca aaggtaatat taaagcacgt
    51301 cagcgaatga taagtcaata tgccattgct ggccaaatgg ctggtgctgt tattggaacg
    51361 gatcatgcgg cagaaaacat cactggtttc tttactaaat ttggtgatgg tggagcagat
    51421 attcttcccc tctttaggct taacaaacgt caaggaaagg ctcttttgaa agttttagga
    51481 gcagatgctg ctttgtatga aaaagttcca actgctgatt tagaagatca aaaaccaggc
    51541 ttggctgatg aggttgccct aggggtgacc tatcaagaca tcgacgacta tttggaaggg
    51601 aagctgattt ccaaagcagc tcaagcaact attgaaaaat ggtggcataa aggacaacac
    51661 aagcgtcacc tgccaatcac catttttgat gatttttgga aataagaaat gatgttcaaa
    51721 gagtaaaaaa agaaaacaaa aaaagttagg ttatcctaac ttttttcttg aagaaaatca
    51781 aataaggtct ataatagaag taatccttta gaatagaatg gctggataat aaccatcttg
    51841 ccaatattat ttcaggtgaa gcttatctta atattttagg aggacaataa tgtcagcatt
    51901 aacagagaca tttacagaac aattatttgc tcattacgaa gccaatgcaa aattttcagc
    51961 tattgagaat gccgttacgc ataatggttt gctaaaatca ttagaaaccc gtcaaagtga
    52021 agtggacaat gattttgtgt tttctattga tttaactaaa gataaggtat ctaaccaaaa
    52081 ggcatctggt cgctgctgga tgtttgctgc cttgaacact ttccgacaca aattaatcac
    52141 agagtttaaa ttagaaaatt ttgaactgtc acaagcccat actttctttt gggataagta
    52201 tgaaaaagct aactggttta tggagcaggt cattgctaca gctgaccaag aattaaccag
    52261 tcgtaaagtt aagtttctct tggatgttcc acaacaagat ggtggccagt gggacatggt
    52321 ggtttccttg tttgagaagt atggagtggt gccaaaatct gtttacccag aatcaatctc
    52381 atcaagtaac agtcgtgaat taaaccagta tttgaataaa ttattgcgcc aagatgctca
    52441 aattcttcgt gacctgattg catcaggtgc taaggcagac caagttgagg atcgaaaagc
    52501 agagcttctt caggaaattt ttaacttctt ggccatgacc cttggtttac cacctcgtca
    52561 ttttgatttt gcctaccgtg ataaagatga ccactatcat gttgagaaag gtttgacacc
    52621 gcaagcgttt tacgacaaat ttgttggctt aaagctttct gattatgtgt ctgttattaa
    52681 tgcgccaact gctgataagc cttatggcaa atcatacact gttgagatgc taggaaatgt
    52741 ggtcggtagt cgtgaggttc gctacctcaa ccttgatatg aaacgtttca aagaactagc
    52801 tatcaagcag atgcaggctg gtgagtccgt ttggtttggc tctgatgtgg gtcaagtttc
    52861 agatcgtcaa aaagggattt tggcaactaa tacttacgat tttgaagcta gtatggatat
    52921 caatcttagt caagataagg ctggacgttt ggattatagt gaaagtttga tgacacatgc
    52981 tatggtttta acaggtgttg acttagatga aacaggaaaa ccgttaaaat ggaaggttga
    53041 aaattcatgg ggtgagaagg ttggtgacaa aggttacttt gttgcttcag atgcttggat
    53101 ggatgaatat acctatcaaa ttgttgtccg taaagaattc ttaacagctg acgagttagc
    53161 ggcttatgaa aaagagccac aagtactagc tccttgggat ccaatgggtg ctttagcaca
    53221 atgctaattc ctttctttgc gactgtatga aatctaaagg ctggacaaaa cgaacagcaa
    53281 acggattgga ctccgcataa gaaagtgaac atgaaaaaaa ttattatgct aagaaaatga
    53341 catatgaaaa aacacgtctt cggtatatgg aaacgtgttt tttcatgttt actctcttat
    53401 ggcattagtt gctatttata acaattttaa ataagtctat tttgttttag gttaacgttt
    53461 gaaaaatcag ttgc
//
LOCUS       DATUYR010000006        47464 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.006, whole genome shotgun sequence.
ACCESSION   DATUYR010000006 DATUYR010000000
VERSION     DATUYR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47464
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.006"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            105..953
                     /locus_tag="VCI33_000296"
     CDS             105..953
                     /locus_tag="VCI33_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP2831251.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(1289..2185)
                     /locus_tag="VCI33_000297"
     CDS             complement(1289..2185)
                     /locus_tag="VCI33_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEP2831252.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYLGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            2483..3235
                     /locus_tag="VCI33_000298"
     CDS             2483..3235
                     /locus_tag="VCI33_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEP2831253.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            3220..4170
                     /locus_tag="VCI33_000299"
     CDS             3220..4170
                     /locus_tag="VCI33_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEP2831254.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKAYYPSIVDATVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKGNKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(4350..6677)
                     /gene="pflB"
                     /locus_tag="VCI33_000300"
     CDS             complement(4350..6677)
                     /gene="pflB"
                     /locus_tag="VCI33_000300"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEP2831255.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            6886..7980
                     /gene="dinB"
                     /locus_tag="VCI33_000301"
     CDS             6886..7980
                     /gene="dinB"
                     /locus_tag="VCI33_000301"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEP2831256.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDVVEPMSIDEAYLDVTNNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGITMTNLEDKVADISLDL"
     gene            complement(8073..8555)
                     /locus_tag="VCI33_000302"
     CDS             complement(8073..8555)
                     /locus_tag="VCI33_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831257.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSILNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTMSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(8646..11009)
                     /locus_tag="VCI33_000303"
     CDS             complement(8646..11009)
                     /locus_tag="VCI33_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEP2831258.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMADALAENLGIESGSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QSTNSNPTENSQSDNDDFRLTPENYSTIDSMIGLTESDIALFFQKKS"
     gene            complement(11067..11660)
                     /gene="lepB"
                     /locus_tag="VCI33_000304"
     CDS             complement(11067..11660)
                     /gene="lepB"
                     /locus_tag="VCI33_000304"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP2831259.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(11671..12573)
                     /locus_tag="VCI33_000305"
     CDS             complement(11671..12573)
                     /locus_tag="VCI33_000305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEP2831260.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNIYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHPFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNTISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            12730..13038
                     /locus_tag="VCI33_000306"
     CDS             12730..13038
                     /locus_tag="VCI33_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831261.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            13041..13586
                     /locus_tag="VCI33_000307"
     CDS             13041..13586
                     /locus_tag="VCI33_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEP2831262.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            13735..16074
                     /locus_tag="VCI33_000308"
     CDS             13735..16074
                     /locus_tag="VCI33_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEP2831263.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADRNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRHKHVKHFAYASQNAGGSGATIVTLG"
     gene            16078..16578
                     /locus_tag="VCI33_000309"
     CDS             16078..16578
                     /locus_tag="VCI33_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP2831264.1"
                     /translation="MESYEHFYAKLSQPFRKKPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            16659..16973
                     /gene="trxA"
                     /locus_tag="VCI33_000310"
     CDS             16659..16973
                     /gene="trxA"
                     /locus_tag="VCI33_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEP2831265.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(17025..17613)
                     /locus_tag="VCI33_000311"
                     /pseudo
     CDS             complement(17025..17613)
                     /locus_tag="VCI33_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(17689..18813)
                     /gene="mutY"
                     /locus_tag="VCI33_000312"
     CDS             complement(17689..18813)
                     /gene="mutY"
                     /locus_tag="VCI33_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEP2831266.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFALYPFATPQKKMLETYLKQKNA"
     gene            19011..19304
                     /locus_tag="VCI33_000313"
     CDS             19011..19304
                     /locus_tag="VCI33_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831267.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            19477..19767
                     /locus_tag="VCI33_000314"
     CDS             19477..19767
                     /locus_tag="VCI33_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEP2831268.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            19789..20280
                     /locus_tag="VCI33_000315"
     CDS             19789..20280
                     /locus_tag="VCI33_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP2831269.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            20445..20684
                     /locus_tag="VCI33_000316"
     CDS             20445..20684
                     /locus_tag="VCI33_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEP2831270.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(20817..21473)
                     /locus_tag="VCI33_000317"
     CDS             complement(20817..21473)
                     /locus_tag="VCI33_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEP2831271.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQKAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGISLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(21606..22550)
                     /locus_tag="VCI33_000318"
     CDS             complement(21606..22550)
                     /locus_tag="VCI33_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEP2831272.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            22753..25581
                     /gene="uvrA"
                     /locus_tag="VCI33_000319"
     CDS             22753..25581
                     /gene="uvrA"
                     /locus_tag="VCI33_000319"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEP2831273.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLREGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVIYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            25697..26770
                     /locus_tag="VCI33_000320"
     CDS             25697..26770
                     /locus_tag="VCI33_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP2831274.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRFIKDTSEIDTIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIDKASAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITETGCQVLTLAPKELIVL"
     gene            26804..27271
                     /locus_tag="VCI33_000321"
     CDS             26804..27271
                     /locus_tag="VCI33_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEP2831275.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGIKKITYKAHYRPHPFAIELMEKKGVAYVQHDVPHIILGGKEGPNG"
     gene            27359..27916
                     /gene="efp"
                     /locus_tag="VCI33_000322"
     CDS             27359..27916
                     /gene="efp"
                     /locus_tag="VCI33_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEP2831276.1"
                     /translation="MIEASKLKAGMTFESEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            27962..28351
                     /locus_tag="VCI33_000323"
     CDS             27962..28351
                     /locus_tag="VCI33_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP2831277.1"
                     /translation="MTTEHIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            28344..28796
                     /gene="nusB"
                     /locus_tag="VCI33_000324"
     CDS             28344..28796
                     /gene="nusB"
                     /locus_tag="VCI33_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEP2831278.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(28937..29902)
                     /locus_tag="VCI33_000325"
     CDS             complement(28937..29902)
                     /locus_tag="VCI33_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2831279.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINQKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(29907..31346)
                     /locus_tag="VCI33_000326"
     CDS             complement(29907..31346)
                     /locus_tag="VCI33_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEP2831280.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGHYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            31588..33471
                     /locus_tag="VCI33_000327"
     CDS             31588..33471
                     /locus_tag="VCI33_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP2831281.1"
                     /translation="MDNRQIATEVIEALGGRENVRSVAHCATRLRVMVHDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSTLGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGHLLQYLVTMLVGLGVAFGIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            33702..36716
                     /locus_tag="VCI33_000328"
     CDS             33702..36716
                     /locus_tag="VCI33_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEP2831282.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKAGQESQKVKEILAKAQQADKQAQELAKMKIPE
                     KIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVNGEASDENLKRSIDVFEEIGKLIGPKGADKSRLF
                     IMDSTYMADKNPLIERGAPYIDLLLVQVYGARGEQGEFQNDTKLVTDTPEERWQGYSK
                     YIRPEQYMIGFSFYEERAGSGNLWYDINSRKDEDKANGINTDITGTRAERYARWQPKT
                     GGVKGGIFSYAVDRDGVAHQPKQIAEKDKQNVKNNQPQIPEITDNIFHSDYSVSKALK
                     TVMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKGDLERFNGILRLDNPAIQSLEGLN
                     KFKKLAQLDLIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSL
                     KVSGLTGLKELDLSGFDRETLAGLDVATLTSLEKVDISGNKLDLAPGTENRQIFDTML
                     STVSNHVGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVANGKVDLQSQLLFGTVTNQG
                     TLINSEADYKAYQNQQIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLAT
                     DKEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADPVNARKVFD
                     GQLGSETDNISLGWDSKQSIIFKLKEDGLIKYWRFFNDSARNPKTTNKPIQEASLQIF
                     NIKDYNLDNLLENPNKFDDEKYWITVDTYSAQGERATAFSNTLNNITSKYWRVVFDTK
                     GDRYSSPVVPELQILGYPLPNADTIMKTVTIAKGLSQQKDKFSQKMLDELKIKEMALE
                     TSLNSKIFDVTAINANAGVLKDCIEKRQLLKK"
     gene            36980..37864
                     /locus_tag="VCI33_000329"
     CDS             36980..37864
                     /locus_tag="VCI33_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP2831283.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFLTTTPEETIART
                     IAYFKAFEADLAGMAIGSFGPIDIDPSSETYGHITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     PHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMTQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            37959..38912
                     /gene="manA"
                     /locus_tag="VCI33_000330"
     CDS             37959..38912
                     /gene="manA"
                     /locus_tag="VCI33_000330"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEP2831284.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATIVLDNMVETTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
     gene            39046..41565
                     /gene="secA"
                     /locus_tag="VCI33_000331"
     CDS             39046..41565
                     /gene="secA"
                     /locus_tag="VCI33_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEP2831285.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMVSLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
     gene            41681..42037
                     /locus_tag="VCI33_000332"
     CDS             41681..42037
                     /locus_tag="VCI33_000332"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEP2831286.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWAGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            42034..43134
                     /locus_tag="VCI33_000333"
     CDS             42034..43134
                     /locus_tag="VCI33_000333"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEP2831287.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKAVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            43221..44732
                     /locus_tag="VCI33_000334"
     CDS             43221..44732
                     /locus_tag="VCI33_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP2831288.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSNNKA
                     EAENQVDDKSTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEARTPIQSLSPY
                     VSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKSTGIAYD
                     STRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAFDLDPTQ
                     ASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFTDTTGSG
                     KRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLGYQASSY
                     AWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKAGYALSKTPKVGYAISFAPGQAGAD
                     GTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSKN"
     gene            45222..>47464
                     /gene="shr"
                     /locus_tag="VCI33_000335"
     CDS             45222..>47464
                     /gene="shr"
                     /locus_tag="VCI33_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEP2831289.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKTVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVTITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLKKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEIS"
BASE COUNT        14654 a         8410 c        10009 g        14391 t
ORIGIN      
        1 acttaatgat cattttattt attattttat tacctttaca ttcatcaata agcctgttaa
       61 aatggtattt gttatatttt attatttgga ggaaaccaga aaacatggaa atgacatgga
      121 ctgtgaaata catcacagaa tttatcgcaa cggctttttt gattattttg gggaatggag
      181 ctgttgctaa cgtcgactta aaaggaacaa aaggacataa ctcaggttgg ttagtgattg
      241 cttttggcta tggcttagga gttatgatgc cagctttgat gtttggtaat gtatctggaa
      301 accatattaa tcctgctttc acagttggac tagcagtatc aggcttgttc ccatgggctc
      361 acgtcttaca atacgttgtg gcacaattac ttggagctat ctttggtcag ttggtagtgg
      421 taatggttta taaaccttac tttatgaaaa ctgaaaatcc aaatcatgtt ttaggctcat
      481 tctcaacgat ttcatctctt gataatggtc aaaaagacag ccataaagtc tcttatatca
      541 atggtttctt aaacgaattt gtaggttcat ttgttctttt ctttggtgct ttggctttga
      601 ctaaaaacta ctttggggta gagttagttg gtaaattgat tgaagcaggt tacgaccaaa
      661 caactgcagc aacacaaatt tcaccatacg tgacaggctc attggcggtt gctcacattg
      721 gtattggttt cttagttatg gtcttggtta cttcattagg tggaccaact gggccagcac
      781 taaacccagc gcgtgatttt gggcctcgtt tgttacacca cttcttacca aaatcagttc
      841 ttggtcaagc caaaggtgat tcaaaatggt ggtatgcatg ggttcctgta gttgcaccta
      901 tcttagcggc gatagtagcc gtagcagcgt ttaaatacct ttatatcaga taaaaaaaga
      961 actaaagctt cttttagact caagacaagc tcgcttaagg aacttgtctt ttttatatga
     1021 gttaagcgaa atctaatgca aacttgactg tcttgtatag cgtattttgt aaattttact
     1081 agacttcttg taaaaaagcc tctcctcaga taagatgact aatgcctcta aacggtgtta
     1141 caaataaacg aggcatccct aacttccttt atctggtgtt gaaactattt tgcacggact
     1201 aaatcactga tagagtaaaa aacgattttt catttttgtt gtaaaatatt aaaaacgtct
     1261 ctggttagtg attcccagaa acgttatctt atctatttgt ttgttgctta tgagaggtaa
     1321 aggaggaacg aatcagcatt ccaccagtga agaaaacgat agcgatgacc agaagtttca
     1381 agctattaat atctaaattt gttaagaaaa acacagctag taaaacgcca ataataccac
     1441 cagaaacaat tccagcaaaa ccatcccaac tgacacggtt tgctttgata aactggctac
     1501 tagaagctgt cataatcata gctgcatcta gcatcataac aggcatagca actgttgggc
     1561 taagacccat taatgaaaag aaaatcaatt ctggagcata attgcctaac cccatagtca
     1621 tcaagactcc aacgataaaa ttaaaaccaa ttccaacaaa aagccagatg ccatggaggc
     1681 catgaatggt gtctgaaagg tgttctcctg gatggtagat tattctaatg atcatgatta
     1741 tagctgctat gattaaaaga ctaccaagga ttcgttggac agttggagca tgccagtttt
     1801 ttgtaatatg agtccccaaa taagctccaa taaaggctgc tgcagccata gcaagtaaag
     1861 ttagaacctc aacttttacg acaaaaataa agcaaagaga ttgaatgagc accggaagca
     1921 catgagctac ggtcatagtg ccaggcaatt gtctatcgtc agtgactaat ttagttaact
     1981 taaagcacgt tgtggtagta gcaaaagacc ctatcccaag ggtgtcaagg gcatctgtta
     2041 gcaggccaat ccaaaacccc gtccaaaagc gtttaaaagg attgatctta ttttttttaa
     2101 gatgttttac taacataaag aaaataaaaa gcattgccga aactagcaaa gcttgtatca
     2161 gtctaaggat aagcatttcg ttcataaaga ctagtttaca aaaaaaaacc ttagccgtca
     2221 acgtgattta cttcattaaa acatgccact atgcttagaa gagctttggt tcgatgtatt
     2281 ttgttaaaat taagctaaaa tattaatatt gcatgaaagg ggacgccttc ctttttccct
     2341 tactgagctt agctgttatt gtagtgaatg tttaaaaggc tagtttgtcg ctctttatca
     2401 aaaatctagt aggatgaata cgtataattt cttgtcaaat agaggaattt tgtctataat
     2461 agcagaggat taggaggaaa ttatgacttt aatggaaaat attaccttta aaactccagt
     2521 attacgcgtt aatgatcgcg accttaatat tgcattttat caaaataatt tagggcttcg
     2581 tttagtatct gaagaaaatg cgattgctat cttttcatct tggggagaag ggcaggaatg
     2641 ttttgtaatt gaagagtcac cttcagttag aacgcgtgcg gtagaaggcc ctaaaaaggt
     2701 taatacgatt gtcatcaaaa caaatcaacc taaagaaatc gaacaattat tagctcatgg
     2761 agcacattat gacgctcttt ttaaaggtca aaatggctat gcctttgaga ctatttcccc
     2821 agaaggagac cgattcttgt tgcatgctga acaagatatt aaacatctcc aaggtactga
     2881 tttaccatcg cttgaaaaag atgcaacttt taaaggttta actcaattta agtttgatat
     2941 tattgtttta aatgttatta gtgaagaacg ctcaaaagct ttttatcggg atctgtttag
     3001 tgatcagtta cctattacta tggattttat tcaagaagaa ggtcccgatt tggcaattga
     3061 tccccatatt gcttgggatt tagaaatttt agaatttcaa gtctcaaagg actatgatat
     3121 gaaggtccta aaagcaactt tagaagaaga cgggcacaag gtctacattg ataaaaaaca
     3181 caaagtattg gtcctttctg atccaagtca gatcgaagta tggtttacga aatgactttg
     3241 gcagtcatta aatgtattga aaaccatctt cacaaaaaag tatacaaagg ggccagtctg
     3301 gctctctttc aatctggtcg atggcaagaa taccatatcg gaacaattga tggaagacga
     3361 ccagttgatg ctaacttagt ttatgacttg gctagtgttt caaaagttgt tggtgtggcc
     3421 acaatatgca atattttatt gaataatggt acgttagcat tagatgatcc cttaaaagca
     3481 tattatccta gtattgttga tgcgactgtc actattcgac agttattgac tcataccagt
     3541 ggtcttgatc cttatattcc taacagagat gttttaaatg cacaacaatt aagaaaagca
     3601 cttaatcatc ttactcaaaa aggaaataaa aacttttatt acacagatgt caatttcctc
     3661 ttgttaggct ttatgttaga agagctcttt agtgaatctt tagaccagat atttgacaag
     3721 actatcttta ctccctttgg gatgtatcat acgtcttttg gccctcgtcc agaagctgta
     3781 ccaacattaa agggtgtgag tgatggggaa gttcatgatc ccaaagcaaa aatcttaaaa
     3841 aaacattctg gatctgcggg gctattttca actcttgcag atttagaaag ttttagcaat
     3901 cattatctta atgatccatt ttcagattgt ctgtggcgca actacagcca acaaacaatt
     3961 gagcggtcac tagggtggaa tttagatggc gattggatta gtcatacagg ttacacaggg
     4021 ccgtttctaa tgcttaataa aaaagagcaa acggcagcta tttttttgac caaccgtact
     4081 tacgacgagg atgataaaag taaatggtta aaagaacgtc agttgcttta caatgcacta
     4141 aagcatgatc tgacaacacc cgtatcgtaa cgtctaaagg ggcagtttta gcgatcttat
     4201 tactgttttt aaagtttctt ctaaaaaata gccatttcat ttgtctgatg agattagagt
     4261 gattataagt gaaaaaagaa cagccatctc attttgaatc aaatgagatg gctgttcttt
     4321 tttataagtt attgatcatt tagatactac tatttacttg aaatagcttc agcggcatca
     4381 tccattgaaa gcacttcgtg gaagacacgt tgagtcaatt cagttttttg ttctggtgta
     4441 aggtatttgg tgttgacaca gtaaccagag atacgcacga taacgtcttc gccgttcatg
     4501 attttgtcat aaacatcgtt aaggtccata acgttcaagt taacgtgctg accaccattt
     4561 tcaaagtaac cgtcaagcac tgttaccaag ttgtcaactt gttcgtcaaa tgttttacca
     4621 agagcacgag gtgatacttg agtagtcaat gagataccat cgttagcatg tgagaattcc
     4681 agtttagcaa gtgagttaag gttttgtaac cagccacctt ttgctttgtt agatgggtta
     4741 gcacctggtg agaagaattc cacttggcta gtgttaactg taccatcttc gtttaagaag
     4801 acgccgcggt gaactggaga gttaccagtt tgtttagagt aagcaacgtt tgaagtgatg
     4861 gtaagaagtg atactgaagc ctcagcattc ttgtaaagtt tgtggcttgc aagacgtgtg
     4921 tggtaagctt ccatcaacca tttcgcgatg tcatcaacac ggtcgtcatc ttcaccgtaa
     4981 cgagggaagt cacctgttac ttcgtagtca tagatgtagc cattttcatc gcggatagtt
     5041 ttaacagttg cgtatttaat agctgacaag gtatcaactg tgttagcgaa accacagata
     5101 ccgaatccca tgttagcacg ttggtgtgtt ggcaagaagg ccatttgaac tgcttcgtag
     5161 ttgtatttat cagtcatgta gtggatgatg ttaagcgcat ctacataagt atctgtcaac
     5221 cagtcaagag atttctcaaa gttagccata acttcgtcgt actcaaggac ttctgatgtg
     5281 attggttcaa caacgttaaa cactttgtag tctctgtgaa catcatcgta accaccgtta
     5341 agaccagtaa gaagggcttt gagaacgttt acacgagcac cgaagtattg gatgttatga
     5401 cgttgttctt cattttctgg gtcaagtggt gatacacaac atgaaataca tgacatttca
     5461 ccataacctt ctttagccat tgttgtcaca ccttcatatt ggatagaaga gtgtttgtgg
     5521 ctcattttca tacagtagcg acggaatgtt tctggcagtt ggtcagtcca aagaactgtt
     5581 aagtttggtt ctggagagtt accgatatta tcaagagtgt ttaagaaacg gtagtccatc
     5641 ttagtcacac ggtgacgacc gtcgttaccc ataccagcca tagaagttgt gatgaatgtt
     5701 gggtcacctg agtaaagggc atcgtaagct ttagtacgac caaattttac ggtacggagt
     5761 ttaaggacaa aatcatccac aaattcttgg atttctgatt ctgtaaaggt accgcgagca
     5821 aggtcacgtt ctgcaaagat atcaagaacg attggcacac gtccaagaga agtagcagca
     5881 ccgttgatca cacgacatac agccatgaaa gcgatgttta cccattggat agcttcttta
     5941 acgttttgtg ctgggcgacg aacatcaact ccgtaaaggt caccaagttt aaccacttca
     6001 ccaagagctt ggtattgaag gttaacttct tcgcgaagac gaatggattc ttcatcgatt
     6061 tcagtgatag cattccagtc attgactttt tcttgcatca agtagtcagc accgtaaaga
     6121 gcaagacgag cgtaaacacc gatgatacgt ccacgtgagt aagcatctgg aagaccagtt
     6181 acagtgtggg catgacgagc acgacgaata tttgaagtat aagcgcggaa gataccgtcg
     6241 ttaacagttg tcacgtactt agtaaagatt tcgtgtactg ctgggtctgg ttcatagccg
     6301 ttttctttaa gggtcgtttc cgccatacgg ataccacctt ttggcatgaa gttcaatttg
     6361 aacaattcat cgttttgaat accataaatt aattcatttt ctttatcgat gaaaccagca
     6421 ggaatgtcag caattgaagt tggacgagta tcgtatggga aacgtgtagc ttcatagtgt
     6481 gcttttgttt cttcgataac ttttttgatg tgaagtgaac gttcagtagc accagccaag
     6541 aaagattcat cgccatcgta tggggtgtag ttagcttgta caaaacgtga gacacttgct
     6601 ttttctttcc agtcagttcc tttaaagcct tcccaagctt tttcgaaaac atctgtattg
     6661 gttttaacag ttgccataaa tttctcctta aattctagta acaaagcgat ctgttacatt
     6721 tacaattcta agtttaacat ataggaaacg ttttcccaag caaaattctt gacaaaatta
     6781 agattctttt ataatacaga ttgttgaaac agtcacaaac tgtattagaa tgtatttttt
     6841 tggctttaga tagcctaaaa ggtataatag agaaaggagg gacttatgct tatttttcca
     6901 ctgattaatg acacgtcacg aaaaatcatc catattgaca tggatgcctt ttttgctgca
     6961 gttgaggaaa gggataaccc tgctttaaaa ggaaagcctg ttgtgattgg gaaagatcca
     7021 agagaaacag gtggtcgcgg agttgtttcc acttgtaatt acgaagcgag aaaatatggc
     7081 attcattcgg ccatgagctc taaggaagct tatgagcgtt gtcccaaagc catttttatt
     7141 tcaggaaatt atgaaaagta tcgaacagtt ggggaccaga tccgccgtat ttttaagcgt
     7201 tatactgatg tggtagagcc catgtccatt gatgaggcct atctagatgt caccaacaat
     7261 aagttgggta taaaatcagc cgtcaaaata gccaagctga ttcagcatga tatctggaaa
     7321 gaagtaggat tgacctgttc agcaggtgtg tcttataaca aatttttggc taaattggct
     7381 agtgattttg aaaaacccca tggcctcacc ctagtcttga aagaagatgc cctgtgcttt
     7441 ttagccaaac tccccattga aaagtttcat ggtgttggta aaaaatcagt tgaaaaactg
     7501 catgacatgg ggatttatac aggacaggat ttgttggcag ttcctgaaat gaccttgatt
     7561 gatcattttg gtcggtttgg ttttgacctt taccgtaaag cgagaggcat cagcaattct
     7621 cctgtcaagt ctgatcggat acgcaagtca attggcagtg agagaaccta cgctaaactg
     7681 ctttatcaag aaacagacat caaggcagag atcagtaaaa atgctaagcg cgtggccgct
     7741 ctcttacaag accataaaaa gttaggcaag actattgtac tcaaagtgcg ttatgctgat
     7801 tttaccacct tgacaaaacg tgtcaccttg ccagaattaa ccagaaatgc cgcacaaatt
     7861 gagcaagtag ctggggatat ttttgacagc ttaagcgaaa atcctgctgg tatccgcctg
     7921 ttagggataa ccatgaccaa tttggaggat aaggtagctg atatttcctt ggacctatag
     7981 gagaaagagt gctctatccg tccgcctcat tttggagaaa aaagaagctg attagggatt
     8041 tccctaatca gcttctttgg tccattattt tattacagca tagcttggtc tgtatcatca
     8101 cctttttcgt taagggtttt agccttgtaa aaagcaaagc cataaagagc agcttgtata
     8161 aggataagga tagttccaac cattgacatg gttgttgtga actggttaac caagccgata
     8221 acagaaagga taaggcctaa gatataagga atataactaa ccgttagacc ttgttttaat
     8281 ttagataagt tttgagcaca aaagacaatg atagcaataa tagcaaggac atttaagata
     8341 gataagaaaa tcataaatgg tgtcatgctg ctttgtagct gtgccaattg ctcagttgtt
     8401 aattgggccc gaaatgcttc attcttgagt tggctttgta agtagaaaat ccctgcaaat
     8461 cccattaatg acagtacaac tccaagcccg tttagaataa taatggcaat agtagacgtt
     8521 ttgagtgcct gacgcacttt ttcataagaa atcatataat cctcctagag ttcttaatcc
     8581 gaaattgtac aatgtgttta atacaaaaat attataccat agattaatca aaaatgtccg
     8641 tttttctagg attttttctg gaaaaagagg gcaatatctg actctgtaag ccctatcatc
     8701 gaatcgatag tcgaataatt ctcaggtgtt aacctaaaat catcattatc tgactgagag
     8761 ttctcagtgg ggttagagtt tgttgattga gaggaggcta agtcggattg ttcttggaag
     8821 cgttcgatca agtaggtttg gcgtttatcg ccttcgtgtt taatagcata ctcaaaggca
     8881 gtatattctc ccaacagaat taacttactt ttagaccgag taatggccgt gtaaatcaca
     8941 tttcgttgca agagtcggcc gctttggcgt gtgataggta aaatcaccac ttgaaactca
     9001 ctcccttgcg atttatgaat gctcatggca taggccaggg ttaattttag ccattcattt
     9061 ctagggtacg tgacttcgct accgtcaaaa tctaagatta attcgtcttg cttagattcg
     9121 gtgtatttag caggaatcaa atctgtaata taaccaatat ctccgttaaa gacattcaac
     9181 tgagcatcgt tgactaagtg caggacttta tcacctttac gaaaatgcgt atcattgaac
     9241 agaaattctg tttgtccttg caagggattg agaagttctt gcatgagttg attaagatga
     9301 ttgatgccag cttgtccttt gtacataggc gctaaaattt gaatttcctc agcagggatg
     9361 ccacttttaa tagcagatag gacaattttt tgaatcatgt cagggataaa ggctgcttgg
     9421 gcttcaaaat aagaacggtc agctttttta tcacgaaagt cagcagctaa gattcctcga
     9481 cgcatctggt ctgccaaatt gacaatagta gattcttggg attgacgaaa tatcttttgc
     9541 aaggcgattt ggggcaggct attgactttt aaaagatctg acaggacttg accgggtcca
     9601 accgaaggaa gctggtcact atctccgaca ataatcactt gagttgttga attaatagct
     9661 cctaacaact ggttggcaag ccaggtatcc accattgaaa attcatcgac aatcagcagg
     9721 tcgcagtcta gataatcctc catggcttgg taatcgttgt cgccattgag gcctaagtgc
     9781 ctgtggatgg ttgcgctagg aagtccagtc aactcattca tgcgacgtgc agcccgacct
     9841 gttggagctg ctaacaagat aggaaggtct tttttatcca aatcaatctg atgcaggttc
     9901 gcgtaagcct gcaaaatgcc tcgaataaca gttgtttttc ctgttccggg accacctgtt
     9961 aggagaaaga ccttgctggt taaggcctta gtaatggctt cttgctgcac ttggtcatag
    10021 gtgatggcaa agtctttttg aacggcttgg atggttgttt gaatggtatc atgactaaat
    10081 gactgactta aaggggtatc taagagacga gagatgttgt tagcaatccc ttcttcagca
    10141 aaataaaggc tagcatcaaa cagtttggtg tcactgtttt tgattttgcc ctctatgatc
    10201 aactcgctta gttgttcagc cacagcagca ggatcacatt ctacttgacg tgcatcttca
    10261 aggagtgtaa tggcaaagtc taataattgt cgtgcttgaa cataagtatc tcctcgattg
    10321 atggattctt ccaagagaca gtgaaggagg gctgcacgaa aacgtttcgg agagccgctt
    10381 tcaatcccta agttttcagc gagagcatct gccattttaa acccaaaacc ttgtagatct
    10441 tcgactaact gatagggatt ttctttaaca aggtcaagag cctcttcttt atacttttca
    10501 aacgcttgaa aggcaaaacg gttgctaagg ccaagttcga cgagaccagc aatcagttgc
    10561 tctgtgccgt aattcaattt tagtttagcg acaaaggctt gacgattagc cttagatagg
    10621 ccagagatag tttctaattt gcttgggtct tctaaaatat ggtcaatggt attatgacca
    10681 tatagtgcaa tgattttctc cgctgttttt ttaccaatcc ccttaaaatg gtcgctagag
    10741 aaataattaa ccaaaccaga tgaactaggt ttgatttttt ggtaacggct tagtttgagt
    10801 tgctgtccat atttagggtg ctgggtcaat tctccccaaa aggtgtagtc atctccttca
    10861 ataatgtcag ccatcgttcc tgtgataatg atttcaaagt cgtcaatgtc actgtctgta
    10921 tcctcaatgg caaggaggag aatcttaaag aaattagcct ggttttcaaa aatgatacga
    10981 tcaacagtac ctgtaaaaac gtattccata gtaactcttt ctatttatac aagataactg
    11041 aaaacctgcc caaaggcagg ttttttctaa ttaaagacgg tcattttatt gagtggccaa
    11101 aaacgagctt tcacttcacc gataaggttt tcttttttga aactaccaac ttcgcgacta
    11161 tccctggaaa caatacgatc atcaccaaga agaaggtatt ctccttttgg aacactcatc
    11221 tcaaagcgtg tttgtccctc agaattagtt gtaaaagcat cagatgtttc tgctaactgt
    11281 tggaataggg tattataggc gtaagttttt tggagtttat cgtttttaaa ttgttttaga
    11341 tactcagcca aatacggctc aactgttttt ttaccattaa tataaagtgt gtcatcatta
    11401 taagaaatgg tatcgcctgg caatccaata actcttttta cgatttcttt cttttgtcca
    11461 ttttcttctt cctgagcaac tacaatatca aagcgatcaa tcctagcttg attaaaaacg
    11521 ataaggcgtt cgccatgagc tagagttggg tccatagaat ggccgtctac tttaacagcc
    11581 tgccacaaaa aaagacgaga tagaccaaag agaattaaaa agagagtgaa tgggccccat
    11641 tctttaataa actgtttcat atgattcctt ttatttagtg agaagcgctt gcgctttgtg
    11701 agtgttggcg aagtgaagtt tagcactata ttctaagcta gacataccat aagcggctaa
    11761 gagttggcta gcaactttat ctgaagctga accagcaccg cttgggagtt ggtaacctaa
    11821 atcttgacct aattgatcga gattgtctaa aaatagattt ctagcaatga tagagctaac
    11881 agccacagcc aaatagtgac tttctgcctt ttcttgaaag gttagaggat ttgggaaatg
    11941 gtttttttcc ttttttagat gcttttcata atttgattgg ctagtgaaag catcaataac
    12001 aatttgttta ggttgaatgc ctttttgtaa caacaaaaaa atagcttggt tatgaagagc
    12061 aactttgata gaaatggtat tatatggttt acttttacca accaattcgt tatatttttt
    12121 aggtgatagg agaagtgatt gatgaggtat ttgtttctct aacagaggcg caatctgttg
    12181 aatggtttta tccgataatt ttttagaatc atcaacccca agctttttca ggaagggatg
    12241 atctttagga tcgacaaaac ttgccacaac agcaatccca ccaaaataag agccattacc
    12301 cacctcatca ctgccgatta taggtatatc ttggctattg ttgcttgctt caaaaactgt
    12361 cttagctact ggaagattaa gctcttgagc taaagcattt gcaccgtttc cctggagaac
    12421 taattttccg gatttgtaca gcaaaacagt aaccccattt tttttagcag caaaggctac
    12481 atagatattt tggctactga tggtgtaggg ggctagctgt ttttttaaat gtttactcag
    12541 tatagcatct attttcaaaa ctaaggtatt cataataatt attttaacat aaaatagttg
    12601 actatggtta attcataagt ttatttaaca aagaaaacag agactcgaat gtttagacgg
    12661 gttagggcaa tggtgttgac tattttacga aagtttcatt ttacggtata atgctaatga
    12721 ggtgacccta tgaaaagtat taatcgctat aaattcacct ttggtgaaaa aacattaaca
    12781 ttgacaactg acaaagacaa tctctttatg gaagaagttg agcgggtagc taaagaaaag
    12841 tatcaggctc ttaaaaatca tttaccagaa gctgatgacg agacaatcgc tattttaatg
    12901 gctatcaata ccttgtcaac acaattaagc agagagattg ccattgaaaa aatggaagct
    12961 gaaattttag atttacgtca aaaaaccttg gttggtcttc aagaaaaagc taatcaaata
    13021 gatgtgaatg aggtttaatc atgttatctt tattgatagt gcttgtttta acttggaatt
    13081 tttatattgg ttatagtaga ggtattattc ttcaatcgtt ttatgtctta ggagccctac
    13141 tttctctttt ggtagctaat cgattttata ttggattagc ccataagcta acgctttgga
    13201 ttccttattc taacccggta gaaggaacat cagtcttttt ctttaaatca gttgatatct
    13261 ttgttttaga taaggtttat tatgcaggcc ttgccttttt tatcattttc ttattggggt
    13321 atgcactgag tcgctttctt gggatttttg ttcatttcct tttgctagat tattttgata
    13381 accagtggac aaaatgtttg agtggcggac tagcgcttct agtatcgctt ctttttttga
    13441 acatgttact gtctattttt gcaacggtcc ctatgccttt tttacagcac tatttgcact
    13501 ctagttttct tgctcgtttg gtgattgaac accttccacc attgacaata attattcaaa
    13561 aattgtggat tcaagccatt atttgatatc gttttgtgac taatataaga acctggagtg
    13621 agtttcacct ttaggttctt tatttacgag aaactgttta gaaataaaga gaacgcatgg
    13681 gaacttagtg actttcttgc cacattttcc atcattttag gtataatgaa gagtatgaat
    13741 aacaagattt tagagcagtt agaatttaac aaagttaagg aattgctatt accttatctc
    13801 aagacagaac aatcacaaga agaattatta gagctggagc cgatgacgga ggctcctaaa
    13861 atagaaaaaa gttttaatga aatttctgac atggaacaga tttttgttga acatcactca
    13921 tttggcatag tcagcctaag ttcaatctct gagagtttaa aacgcttaga gctttcagct
    13981 gatcttaata ttcaagaact tttggctatc aaaaaagttt tacagagttc ttcggatatg
    14041 attcactttt attctgattt ggataatgtt tctttccaat ctttggatcg tttgtttgaa
    14101 aatttggaac aattccctaa tctgcaaggg tcttttcaag ctatcaatga tggtggtttt
    14161 ttagaacatt ttgcgagtcc agaattagag cgtatccgtc gtcaattaac aaatagtgaa
    14221 cgacgggttc gtcagatttt acaggatatg cttaaggaaa aagcagagct tttatcagag
    14281 aatctaatcg ctagtcgtag tggacgaagt gtcctaccag taaaaaatac ttatcggaat
    14341 cgtatttctg gtgtggttca tgacatctct tcttcaggaa gtactgttta tattgagcct
    14401 cgtgctgtag ttacactaaa cgaagagata actcagctta gagctgacga acgccacgaa
    14461 gaaggtcgta ttttacacgc attttcagac ttgttaagac ctcatgtcgc cactattaga
    14521 aataatgcat ggattcttgg gcatcttgat tttgtaaggg ctaaatatct ttttatgtct
    14581 gataataagg cgacgatacc taagatctct gatgacagca cgttagcatt aatcaatgtt
    14641 cgtcatcctc ttttaagtaa tcctgtggct aatgacttac attttgatca cgatttaact
    14701 gcaattgtca tcactggtcc caatactggt ggtaagacga ttatgctaaa aacactcggt
    14761 ttagcacaat taatgggaca gtctggtttg ccagtattag cggataaagg tagtaaaatt
    14821 gcagtattta acaatatctt tgcagatatt ggcgatgagc aatctattga acaaagtcta
    14881 tcaacttttt ctagtcatat gacgcacata gtcagtattt taaacgaggc tgaccgcaat
    14941 agtttagttc tctttgatga actaggagca ggaacggatc ctcaagaagg tgctagtttg
    15001 gctatggcta ttttagaaca ccttaggtta agtcatatca aaacgatggc gaccacgcac
    15061 tatccagaat taaaagctta tgggattgag acaaattttg tagagaatgc gagcatggaa
    15121 tttgatgccg aaacgcttag ccccacgtat cgctttatgc aaggagttcc tggacgatca
    15181 aatgcatttg aaattgcttc tcgccttggt ttagctccat ttattgttaa acaagctaag
    15241 cagatgacag attctgactc agatgttaac cgtattattg aacagttaga ggcacagaca
    15301 cttgagacac gtagaagact ggatcatatt aaagaagttg aacaagaaaa tctcaaattc
    15361 aatcgtgcgg ttaagaaact ctataatgaa ttttcacatg aacgcgataa agagttagaa
    15421 aaaatctatc aagaagctca agaaattgta gatatggctt tgaatgagag tgatactatc
    15481 ttaaaaaaac tcaatgataa gagccaatta aaacctcacg aaattataga tgctaaggca
    15541 caaataaaaa aattagcacc tcaagttgat ttatcaaaaa ataaagtctt aaataaggct
    15601 aaaaaaatca aagcagctcg tgcccctaga attggtgatg atattatagt gactagttat
    15661 ggacagcgag gtaccttaac tagtcaatta aaagatggcc gttgggaagc acaagtggga
    15721 attatcaaaa tgacattaac acaagatgaa tttagcctcg ttagagtcca agaagaacag
    15781 aaagtcaaaa ataaacagat taatgtggtt aaaaaggctg atagttctgg accaagagct
    15841 cgacttgatc ttagaggtaa aagatacgaa gaagcaatgc aagagttaga tcattttatt
    15901 gatcaagcat tgcttaacaa tatgggacaa gttgatatca ttcatggtat tggtacaggc
    15961 gttattcgtg agggagtgac aaaatatctt cgtcgtcata agcatgttaa gcattttgct
    16021 tatgcctcac aaaatgcagg gggatctggc gccacaattg taacgttagg gtaaatgatg
    16081 gaatcatatg aacactttta tgccaaacta tctcaacctt ttagaaaaaa accacagtta
    16141 ataattcttt taaatgtttt actaaaaata atcacaggag tgatgtacat actttatcca
    16201 tcttttctga tatttacgct ttggcaaggg atgacatttc aattatggtt aaggttatta
    16261 attattcctg ctgttggctt tatagctttg tcttacatta ggaaacgctt tgattttcca
    16321 cgaccgtatg aaaaatggaa tattaaacct ttaattgata aggatacaaa gggaaggtca
    16381 atgcctagca gacatgtttt ttcagctacg atgattagta tgtgtttgtt acgatattat
    16441 gtttactttg gaatagtttg tttaatatta tcggttttat tggcaatttg ccgtgtgata
    16501 gcaggtattc actatcctaa ggatgttatt gtaggttacc ttattggctt gatgttaggg
    16561 ctttgtttat tgatttagcg agttcgtagc aaatagtttc aaaaacaact aaattcttgt
    16621 acaataggct tatcattaag atgaaatgga gaaaaaagat ggcattagaa gtaacagatg
    16681 ctacatttgt agaagaaact aaagaaggtt tagtactcat tgatttttgg gcaacttggt
    16741 gtggtccatg tcgtatgcaa gcaccaatct tagaacagtt atctcaagaa atcgatgaag
    16801 atgaactcaa aattttgaaa atggatgttg atgaaaatcc ggaaactgct cgtcagtttg
    16861 gtattatgtc aattccaaca ttgatgttta aaaaagacgg agaagtggtt aaacaagttg
    16921 caggtgttca tactaaagac caactgaaag ctattattgc tgaattgagt taactaaaat
    16981 aaagcccagc gtcacgctga gctttatttt atgacctact aacttcaatg gaataaccat
    17041 gtaaacataa aacgacttgc aaaagcaaaa gcaacagtaa tgaggaaaaa tacagtgaca
    17101 ataaggggtt tctgataagg aagttttgct ctttcttctc gtttttcgat ttcgtctaat
    17161 aattttccat ttgaaatagc taggatttcc ttataaggtt gcacatcata ttttttctta
    17221 aaacgttcca cacgattagc ataagtcata gtaattatcg ataataaaac aaaaatgcca
    17281 agtcctagcc actctaagaa atagacttaa tggataactt gaaatcatta atagtatcat
    17341 caatgttacc attactttgg tatctcgttt aaaattcttt ttatcaacct gagtgatggt
    17401 atatttcatt ttctcaatgt ctcctttaat gagttggtct aagctcactt gaaaaatttg
    17461 acttaggagt aagaggctat ggatgtctgg gtaagttttg tcattttccc agttagatat
    17521 gctctgacgg cttacataga ctttctcggc taatttttct tgcgagagcg cttgttgttt
    17581 acggtagcga ataatttgct gaccaatttc cataaatatc accgaaaaac tttacagcgg
    17641 cgtaaaggaa ttgtaaacaa aaagtgaacc gttatagttc acttgcgatt aagcattttt
    17701 ctgctttaag taggtctcta gcattttctt ttgcggtgtt gcaaagggat acagagcaaa
    17761 gtcctcaatt gctacccact ttaggtgagg agcattaggt aaatctgttg ctttgaccac
    17821 cccctctatg agttctattg tccacttctg gtgactaaaa gtgtgcttaa tatttggaaa
    17881 gtgattatca gtccattggg gctttaattg atattctctt tcgaaagttt cattttgggt
    17941 ttgccagatt atggggttgg attgattgtc atcaaataaa tctaattgtt gagaaagagg
    18001 tgacgtttcg ataataggaa atgaccaaaa acctcctaga agtctccctt tagtattttt
    18061 ctccagtaga tattggccat tttggttacg aatgacaaaa gcctgaattc gcattggttt
    18121 aggctttttc tttggatttt ttatggggta tttaccataa gtaccattca aataagcagc
    18181 gttaaagaaa cgaatagggc tttcatcagg cctaggtgtc ttagcggatt cgatgtctgt
    18241 acctagatcc ataagtgcct ggttaaaatc accgggtctg tcagggtcaa ttagtatttc
    18301 cataatagct tgaaaaattt tacgattttt agggtcaccg atatcataat taacttcaaa
    18361 caaacgagcc atgacacgca tcacattgcc atcaacagca ggctctggta agttgaatga
    18421 gatgctggcg atagcacctg cagtataagg accaatccct tttaatgaag caatatcatc
    18481 ataagtgtga ggaaatatgc caccaaaatc aaccatcact tgctgtgcag ccttttgcat
    18541 attacgcacc cgagaataat agcctagtcc ctcccatgcc tttagtaatt gttcttcagg
    18601 agcgtctgcc aaatctttta tttgaggaaa ccactctaga aaacgtttat aataagggat
    18661 taccgtatta acttgtgttt gttgcaacat gatttcagaa acccaaatat aatatggatt
    18721 agtagttctt cgccaaggga gatcacgttt ttcttggtca taccacccta aaagtgtgcg
    18781 acgaaaagaa gcgattgtct cattatccca catattaata ccataatctt ttaattcaat
    18841 catgttgtta ttgtaacaca ggagccttaa aaagagtaaa aaaagatttt aggtatttgc
    18901 gaaaaagaaa aggaaatatt ataataatat taggtttacc tcatctaaat gttgatttag
    18961 aataactgaa attaacgata gaagcagaca ggttttaatt tggaggggac atgttccaga
    19021 aattaaagaa tcttattgat cggtattcgt ggttcgtttt tataatgatg attcttgtgt
    19081 tatttcttaa ctatatttat ccatttgatc cttctttgag cggactacag ttggaatggc
    19141 tgatcatgac gcaagttttt cctggattag gtatcgtatg tgccatggtt gctattattt
    19201 ttaaaaagtt taaccttttt gttttggccc taattttata ttttgcactt tggttaaaac
    19261 ttttccttgc tttttctttt ttcacctatt ttttaggagt ctgactaagt gccaattttt
    19321 aaaactcgtg aaaggaaatg tttgacaaaa cttagtcaag tagaaaaagt tccttgcttt
    19381 taattgacta ttagtgtaca ataagattct gtgagtacac ctcacttact cactccaaaa
    19441 gtagtgagtc attagaccaa aaggaggaaa atatcaatgg ctaaatacga aattctttat
    19501 atcattcgtc caaacattga agaagaagct aaaaacgctt tggtagcacg ctttgactct
    19561 atcttgactg acaacggtgc aactgttgtt gaatcaaaag attgggaaaa acgtcgtctt
    19621 gcatacgaaa tcaacgattt ccgtgaagga ctttaccaca tcgttaacct tgaagcgact
    19681 gacgcagcag ctcttaacga gtttgaccgt ctttcaaaaa tcaatggtga cattcttcgt
    19741 cacatgatcg ttaaacttga cgcttaattc caactaagag ggtagtttat gattaataat
    19801 gtagtactag ttggtcgtat gaccaaggat gcagaacttc gttacacacc aagtcaagta
    19861 gctgtggcta ccttcacact tgctgttaac cgtaccttta aaagccaaaa tggtgaacgc
    19921 gaggcagatt tcattaactg tgtgatctgg cgtcaaccgg ctgaaaattt agcgaactgg
    19981 gctaaaaaag gtgccttgat cggagttacg ggtcgtattc agacacgtaa ctacgaaaac
    20041 caacaaggac aacgtgtcta tgtaacagaa gttgttgcag ataatttcca aatgttggaa
    20101 agtcgtgcta cacgtgaagg tggctcaact ggctcattta atggtggttt taacaataac
    20161 acttcatcat caaacagtta ctcagcgcct gcacaacaaa cgcctaactt tggaagagat
    20221 gatagcccat ttgggaactc aaacccgatg gatatctcag atgacgatct tccattctag
    20281 tcagaatgga ttgaacgaat acgaaattat ttgagttcgg gctaaaaatt tagaacttag
    20341 aatttagatc aactccctag gaaatcaaga tttcttgcca gttttctaaa attcagtcgt
    20401 tttttaagag cccttaacat cataatttat aaaggagaaa atacatggct caacaacgtc
    20461 gtggcggatt caaacgccgt aaaaaagttg acttcatcgc agctaacaaa attgaatatg
    20521 ttgattacaa agatactgag cttcttagcc gtttcgtttc agaacgtgga aaaattcttc
    20581 ctcgtcgtgt aacaggaact tcagctaaaa accaacgtaa agtaacaaca gcaatcaaac
    20641 gtgctcgcgt tatggctctt atgccttacg taaacgaaga ctaataaaca aacagtccat
    20701 ttcggactgt tttttagttg actaggacta gtctaggcgt ataggtgagt tttttctaaa
    20761 agagagaatg atagtaaacg aaaatccttg cgtagacagc aaggatgaaa tgattgttat
    20821 ttgttaacag gtgacatagt gttttgaatg ttgtatttgc gttgtaggta atagcgtaaa
    20881 gcaagggaaa taccaccaat aatgagcaag gcaagaggag gcaactgtgg gttaagagag
    20941 gttggtaaaa aggcagtcgc agagtacaag gcaatccaaa tcaacatggc taatgataag
    21001 gctgcgatga ttttgaacca acttggacga aggctcttat cttttccgag atgacggtaa
    21061 ataaagtaat aagtagcgta catggatgct cctccaccaa aaccaagtgc cagtaaggaa
    21121 atcaatcctg taactgtagc gtttgtgttg aagaaggtca tgatgccatt taaaagggca
    21181 acaattccga tgaatagcaa agaggtatct agccacatca accaaggatt ggtgtttttg
    21241 tcagtttctg ctgctttttg actagggtct tggctaaaag aagctgccca agcagtcgga
    21301 gtgcctaata gatttcgagc ggtgacacct tttttctggt tttctagaat agctggaatg
    21361 gcttcttcga gaatcagctt aatgtcttca tcagacttgc cgtcttggat taatttgttc
    21421 gtagcggtgt ggataaattc ttgatttttt ttggtaagtt cttgtaattc catgggaatc
    21481 ccttctttat atgggttagt taaagtaagt caatgatatt agctagtgtc aaatgagact
    21541 ttggagccag ccgtttgaca cagagttttg tcaggtgtta actacattct acagcttgct
    21601 ccactctaga accatttttt tctaataaag taaattacaa caaaggaact aagtagcata
    21661 gcaatcaagg taatatacca aaaagcatgt tctaggccat ttagtggtaa ccagttgttt
    21721 tggaaattca taccataggc ggagaaaatc acagttggta tatctaaagc catggtcatc
    21781 aaggccagtg ttttcataat agtgttctgg ttgttgttga taatagaagc agtggtttca
    21841 gtcatggcat tcaagacatt ttcataaatg ccagccatct caatggcctg ttgggtttca
    21901 atcaaggtat cttctagcaa atcctcgtcc tcaatgtatt tcttgagaga gcttgttgag
    21961 ctggataatt tcttgacgat gcgttcgttg aatttcaagg aagcctttaa gtagacaatg
    22021 gatttttcaa gttccatcat gtcgataagt tcttcatttc gagtagctgc ttcaagctga
    22081 gcttccaaac gttcgctttg gcgatcaatg gtcctcaagg cagtcagaaa gagctcggcg
    22141 ttgcgatata gaatctgaaa aacaaaacgt gtcttcatga aggtatagaa atttttgacc
    22201 cggcggttgt gaaaatgatc gaaaagggtc atgtcatgca gacaagttgt gataacagca
    22261 ttttccgtta caataatgcc taaaggcata gtaatatagt aacttttatt gttacgttct
    22321 tcgtatatcg ggacgtctac gataattagg gtataatcat cttctacggc aatacgtgag
    22381 gtttcctcca cgtccaaagg ggcgcgcaag tcagaaatat caatgttaaa ttgatcggcg
    22441 atcttcatag actcttcttg ggaagggtta accagtttaa tccaagctcc tggctcgaat
    22501 gtttctattt ctttgaattc aattgccgat gaaagaaaca tttgtttcat gacaagccct
    22561 cctttttgat gattgactgg tagagattga catcaaacct tgataataaa gccttctcaa
    22621 ggattttctg aaaaattctc aatacttatc tattatacca aaattcgcaa agtttggggc
    22681 aataggttat cagaaatttt tgttataata taagaaagct cgatgaggag ggtgccttta
    22741 ggcacattca agatgcaaaa taaaataata attcatgggg caagagccca caatttaaaa
    22801 aatattgacg tggaaattcc acgagacaaa ttagttgtcg tgacagggtt atcagggtca
    22861 ggaaaatcta gtctagcctt tgatactatt tatgcggaag ggcaacgtcg ttacgtagaa
    22921 agtttatcag cttatgctag acagttcttg ggtaatatgg aaaaaccaga tgtggattcc
    22981 attgacggtc ttagccctgc tatttccatt gatcaaaaaa ctaccagtaa gaacccccgc
    23041 tcaacggttg gtacggttac ggaaatcaat gattatttac gtttactata tgcgcgtgtg
    23101 ggcacacctt actgtattaa tgggcatggg gccattacag catcttctgc agaacaaatt
    23161 gtagagcagg tcttagccct ccctgaacgg acacgcatgc agattttagc gcctattgtt
    23221 cgccggaaaa aaggtcaaca caaaaccatt tttgagaaaa ttcaaaaaga tggttatgtc
    23281 cgtgtgcggg ttgacgggga tatttttgac gtgacagaag tgccggagct gtccaaaagt
    23341 aagatgcaca acatcgaggt ggttattgac cgtctggtta ataaggatgg tattcgtagc
    23401 cgtttatttg attcggttga agcagcttta aggctgggag atggctacct tatgattgac
    23461 actatggatg gcaatgaact gctcttttct gagcattatt cctgtcctgt ctgtgggttt
    23521 acggttcctg aattagaacc aagactcttc tcttttaatg cgccgttcgg ctcttgtccg
    23581 acctgtgatg ggctaggcat taagttggaa gtggatttgg acttggttgt tcctgaccca
    23641 agtaagtctt tgagagaggg agctttggcg ccttggaatc ctatttcctc caattattac
    23701 ccaaccatgc ttgagcaagc tatggcaagt tttggggtgg atatggatac accttttgag
    23761 gccttgactg aggaagaaag agatttagtg ctttatggtt caggagatag agaatttcat
    23821 tttcactatg tgaatgattt tggtggtgag cgtaacattg atattccttt tgaaggtgtt
    23881 gtgactaatg ttaatcgtcg ttatcacgaa actaatagtg attatacgcg caatgtgatg
    23941 cgtggttata tgaacgaatt gacctgtgcg acttgtcatg gttaccgttt gaatgaccaa
    24001 gccttgtgtg tgcatgtggg tggcgaagaa ggacctcata ttggtcaaat ttcagaatta
    24061 tcgattgccg accacttgca gttgttggaa gaattggaac tcacagaaaa tgaaagtacc
    24121 attgctaaac cgattgtcaa agaaattcat gaccgcctga ccttcttaaa taatgtggga
    24181 ctgaattatc tgaccttgtc acgggctgct gggactttat caggtggtga aagtcaacga
    24241 atcaggttag cgacacaaat tggttctaac ttatcagggg tgatctatat cttggatgag
    24301 ccttctattg gattgcacca aagagacaat gaccgtttga tcgaaagcct gaaaaaaatg
    24361 cgtgatttgg gtaatacctt aattgtggta gaacacgatg aagacaccat gatgcaggca
    24421 gactggctga ttgatgtggg gcctggggca ggtgagttcg gtggagaaat tattgcctct
    24481 ggaacgccga aacaagtggc taagaataaa aaatccatca cgggtcaata tttgtctggt
    24541 aaaaaattca ttccagtgcc attagagcgt cgttctggta atgggcgatt cattgaaata
    24601 aaaggagcag cgcagaataa tcttcaaagc ctcgacgtga gattcccact gggtaaattt
    24661 attgcggtca caggggtatc aggctcaggg aaatcaacct tggttaatag tattttgaaa
    24721 aaggctgtcg cccaaaaatt aaaccgtaat gctgataagc ctggtaaata tcactctatt
    24781 tctggtattg aacatattga acgcttgatt gacattgacc aaagtccgat tggaaggacg
    24841 ccacggtcaa atccagccac ctatacgggt gtctttgatg atattcgaga cttgtttgcc
    24901 caaaccaacg aagctaagat tcgtggctac aaaaaaggcc gtttctcttt taatgtcaaa
    24961 ggtgggcgtt gtgaggcttg ttcaggtgat gggattatca aaatcgaaat gcactttttg
    25021 ccagatgttt atgtgccttg tgaggtttgc cacggcagac ggtataattc tgagacgctt
    25081 gaggtgcatt ataaagaaaa aaatattgct gaagtacttg acatgactgt cgatgatgcc
    25141 ttggtatttt tctctgctat ccctaaaatt gcccgaaaaa ttcaaaccat taaggatgtt
    25201 ggtttgggtt atgtgacctt gggtcaacca gctactactc tatctggagg agaagcgcag
    25261 cggatgaaat tagctagtga attgcacaaa cgctcgacag gtaagagctt gtatatccta
    25321 gatgagccaa cgactggttt acacacagat gatattgcaa ggctattaaa agttttggaa
    25381 cgctttgtag acgatggtaa tacggttctt gtgattgagc ataatttgga cgttatcaaa
    25441 tctgctgacc atattattga cttaggacca gaaggtggtg tcggaggtgg tcagattgtt
    25501 gccacgggga caccagaaga ggtagctcaa gttaaagaaa gctatactgg tcattatttg
    25561 aaagtaaaac tacaacaata ggattaggag ttcgtttgag ctccttttta atcttttaaa
    25621 tgctctcaaa tcatggtaaa cttaaagtag ttttttcatg ctacaatatt ctcaaaagtt
    25681 accaaaggag ttcgcgatgt caggattttt agagcaacga ttaggtcact gcctaaggca
    25741 gatggcagag aaggggctag aggctcttct agtcacccat ttaaccaata gttattactt
    25801 gacaggtttt tctggaactg cagcaactgt tttgataacg gccaaacgtc gtgttttgat
    25861 cacagattca cgttatacct tgcttgctaa agctagtgtt gagggatttg atattatcga
    25921 aagccgcacg ccgcttaagg ttgtggcaga attgttagag gctgatcaaa tagattgcct
    25981 tggttttgag gaccaggtat cgttttcttt ttatcaggcc atgcaagcag aactgtcagg
    26041 aataaccttg cttgctcagt caggttttgt ggagcattta cgttttatta aggatacttc
    26101 tgaaattgat accattgcta aagcgtgctc gatctcagac aaagcatttg aagatgctct
    26161 tgattttatt aaaccaggga caaccactga acgtgacctg gctaattttt tagattttcg
    26221 tatgcgtcag tatggtgcca gcggcacatc atttgatatc attgtagctt caggctatcg
    26281 ctctgccatg cctcatggac gcgccagtga caaggttatc cagaataaag agagcttgac
    26341 catggacttt gggtgttact acaatcacta tgttagtgat atgacgagga ccattcatat
    26401 tggccaagtt actgatgaag aacgtgagat ttatgctctt gttcttgctg ctaataaggc
    26461 tctcattgat aaagctagcg ctggtatgac ttatagcgat tttgacggta ttccgcgcca
    26521 actcatcact gaggctggtt atggtagtca cttcacacat ggcattggtc atggtatcgg
    26581 gcttgacatc catgagaatc cgttttttgg taaatctgag caacttcttc aagcaggaat
    26641 ggtggtaaca gacgagccag gaatctatct ggataataaa tatggcgtcc gtattgaaga
    26701 tgacttggtc atcacagaaa ctggctgtca agtattgacc ttggcaccaa aggaactgat
    26761 tgtgttgtaa attatcttag ggaaggatta gaaggacgaa gcaatgacaa atcgtttatc
    26821 atggcaagat tactttatgg ccaatgcaga gcttatttcc aaacgctcta cctgtgaccg
    26881 cgcctttgtg ggagctgttt tggtaaagga taatcgtatt attgccacgg gttataatgg
    26941 tggtgtttct gccacagata attgtaatga agcgggccat tatatggaag atggacattg
    27001 catcaggaca gttcatgctg aaatgaatgc tttgattcaa tgtgcgaaag aagggatttc
    27061 gactgacgga actgaaattt atgtaacgca ctttccctgt atcaactgta ccaaggctct
    27121 gttgcaggct gggattaaaa agattactta caaagcacat tatcgtccgc acccgtttgc
    27181 gattgaacta atggagaaaa agggagttgc gtacgttcaa cacgacgtgc ctcacatcat
    27241 tttgggtggt aaggaaggac ctaatggtta gcttgggcga atggtcataa aagatttgag
    27301 cctagggcgc ttttgtggta aaatgaagtg ataatatttt agtaagaggt aaataataat
    27361 gattgaagca agtaagctta aagccggtat gacattcgaa tcagaaggga aattaattcg
    27421 tgtccttgaa gctagccacc ataaaccagg taaaggaaac acaattatgc gtatgaaatt
    27481 gcgtgatgtg cgtacagggt ctacctttga cacaacttac cgtccagatg aaaaatttga
    27541 gcaagctatc attgaaactg ttccagcaca atacctatat aaaatggatg acactgctta
    27601 cttcatgaat actgacactt acgatcagta cgaaattcca gttgctaacg ttgagcaaga
    27661 gttgctttac attcttgaaa actcagacgt taaaatccaa ttctatggaa gcgaagtgat
    27721 tggagtgact gttccaacaa ccgttgaatt gactgttgcc gaaacacaac catctatcaa
    27781 aggtgcaact gtcacaggtt ctggtaaacc tgcaactctt gaaacaggtc ttgtggtcaa
    27841 cgttccagac tttatcgaag ctggtcaaaa actaatcatc aacactgcag aaggtactta
    27901 cgtttctcgt gcttaatcct ttgtcagaac aggttagtct aaagaagaga aaggaaatgc
    27961 tatgacaact gaacatatcg gtgaaatcgt tatttcacca cgagtacttg aggttattac
    28021 tgggattgct accacacaag ttgagggcgt tcattccctt cataacaaaa aaatggcaga
    28081 tagctttaac aaagcaagtc ttggtaaagg ggtttatctc cagacagaag aagatggaag
    28141 tgttacagca gatatttatg tttacttgca atatggagtt aaggtcccga ctgtatctat
    28201 gaatattcaa aaaacagtca aatctgctgt gtacgatatg gcagaagtgc ctatttctgc
    28261 agttaatatc catgttgaag gtattgttgc ggaaaagaca ccaaaaccag atttgaagtc
    28321 actttttgat gaggatttct tggatgacta acagctttca aaattcaaga agagatttgc
    28381 gtgaacgtgc ctttcaggct ttatttaaca tagagatggg agctgagtta ttggcagctt
    28441 ctcagtttgc ctatggttat gataaagtaa caggggagga cgctcaagta ttggagctgc
    28501 cgattttttt attaagtctt gtgacgggtg tcaataatca taaagaagag ttggataact
    28561 tgatatcaac tcatttgaaa aaaggttggt cgttagaacg cttgacacta acagacaaaa
    28621 cgttattacg actaggtctt tttgaaatca agtattttga cgaaacacca gaccgtgttg
    28681 cactaaacga aatcattgaa gtagctaaaa aatattctga tgaaacctct gctaaattta
    28741 ttaatggctt acttagccaa tatgtctcgg aagctccttc agcaaataaa tcataataag
    28801 tcggagcaat cttaggattg tatccgattt tttcaattta tgtacgaata ttaaaagaca
    28861 aacaagcata ctctcgcatc tagaaagagt atgcttgttt gtcttttaga gtaattagtg
    28921 attggttatg ttttgtttaa atacttgctc ctggaagtag cgtaatcggt aaaatatagt
    28981 ctttacttgt tttttctttt ttaatttttt tgatgagtat ttcgacactc aatttagcaa
    29041 tctcatcaat aggttgacga atggtcgcta attgaggaac atattgctgg ataaatgtcg
    29101 ttccatcata acctatgact ttcatatcct ctggaatagt aatgtgaagt tgtttggcta
    29161 ctttcataat gaggatagca gttaaatcat cagaaacgaa aaggccatca ggttttcggg
    29221 ttgccaaaat ggatttaatt tccatttcgc gtctaacagg agataggtta tttggtaatt
    29281 tgatgatttc tgcacttcgt ttgagctggt agttaaaccc tagctggcgc aaccctgtgg
    29341 gcgaatcaga attgtcatta cctgtaatca tgacaatgtt ttgacaaccg tgtttttgca
    29401 aagtttgtgc ggctaattta ccaccttcaa aattatctga agaaataacc ggaatatttg
    29461 gcgccagatt acggtcaaaa gctacaatag gagcttcgac acgctcataa tcctcaattc
    29521 ccaaattatg actggaagaa ataatgccgt ccacttgatt ggccgcaagc atttctaagt
    29581 attcgcgttc tttgacggga ttatgttcac tattgcaaat aatagttttg tagccctgtt
    29641 taaacaattc aatttccaaa tgttcaatta attcagcgta aaagatattg ctaatgttgg
    29701 ggaagataag cccaatgagt tgggttgatt taccttgcaa acttctagcg agattgttag
    29761 gcttgtagcc caattcacgc atggccttat tcaccttatt cactgttttt tgggaaaggt
    29821 agcctttttg gttgattact cgcgaaacag ttgttggact gacacccgcc aaggctgcca
    29881 cgtctgttaa ttttgcgacc acttgcctaa tacctcattt cataataatg tccaaaagct
    29941 tgtccttcta ctacttggat acctgtttgt ccttgggttg ggaaaacacg actagttagg
    30001 actttttgcc cttggttaat aaagatttca agaatggaat tgtccacata gacattaagt
    30061 gtaacatggc cttgtgggat ttggcaagag cgcaccgtac catagtcttg ggcatattga
    30121 acccctgccc gacttcggtc aatgcttaac tgtccttttg ttgtatccac cttcaatctt
    30181 aggccattcc ctttttggtc ggcaaatagg agcaggtcaa gctttttttg gcctggaatc
    30241 gtcaattcca attcataaca gttagaactg tctattttat tatgaaataa ttctgctggc
    30301 cctcgaagat tttgtagggc tgaaacaggt gtttgataga gaattccgtc tttaatcctt
    30361 aattctttca ctaaactaag agctccttga tagtcataac gatctgtcgg ataatcaata
    30421 tctggtaacc caatccaaga cacggctaga accctgccat ctggactact aaaggcttga
    30481 gtggcataag cttcaaaacc aaagtcaaga ttttgaaggg cccccccacc cagcaattgg
    30541 ccagtttctg gattgaagct ttcaaaaatt ttataggtat tggggtagat attttgataa
    30601 tctaaatcag ctttagccag tccttgtggg ctgaaaatca aaacaggctt gtcatcgaca
    30661 aacacaaggt tggggcattc aatcatgtat tccgtgccag aatcgtcaaa gtccaaatcg
    30721 gctataaatc gccagttgtc cacatgattg tccacagctt tgtaaagttt gatttttcct
    30781 ttgccatcta aaccttgggc gccaataata gcatagaatt gaccttgata agaaaaaagc
    30841 tgaggatccc gaaaatgttc tgtaacatca tcgggctgtt caatcaaaac ctgtggtatt
    30901 ttggaaatat ttccttgttt atccatccat gcgccaacct gtaatggtgt tctgacccaa
    30961 ttggcatcgc gaacattacc agtgtaaaag aggaagagct tgtcatcaat agcataggca
    31021 gaacctgagt aagccccatg actatcgtga gcgtggtctg gcagaaggcg gctccttgtt
    31081 tccgtaaaat ggacgaggtc tgtggatgtc atgtgtaccc attgtttcag accatgcgca
    31141 gccccataag gccagttttg ataaaaaaga tggtagtgac cgttgaaata agaaaaccca
    31201 ttggggtcat tgagtaatcc tgtcttaggt tcgacatgaa attggctgtg ccatggggat
    31261 tgggccattt tttcagtaat agcttggtag tcttttgagc tccactcttt gtaagggcgg
    31321 tagcgaatag cttgtggtag atccatcaaa atctccttgt tttatagtcg tcttcaagaa
    31381 tatactaaac gttttctgtt taaaaatcaa gaatttaaag catataaaat aaaaatatgt
    31441 taaacgcttg acataaaaaa tattagtgat aaaatgatag tcaagaagtg aagcggattc
    31501 actagcgttg gtcatgcaca ttcttagcca caaagctggc agaatggatg agggactaac
    31561 accaaatgaa aaaggagact tttaggcatg gataatcgtc agattgcaac tgaagtgatt
    31621 gaggctttag gtggccgaga aaatgtgaga agtgttgccc actgtgcaac tcgccttcgc
    31681 gtgatggttc atgatgaagg aaagattgat aaggaaaaag cagaagctat tgacaaggtt
    31741 aaaggagctt tctttaattc tggtcaatat caaatgattt ttggaactgg taccgtcaat
    31801 aacatttatg acgaagtcgt tgctcttggt ttaccaacat catcaaccag tgagcaaaag
    31861 gcagaagcag gcaaacaagg caatatcttc caacgggcga ttcgtacgtt tggagatgtt
    31921 tttgttccca ttattccagc tattgtagca acgggtctct ttatgggggt tcgtggtttg
    31981 gtgacccagc cagctattat ggatttattt ggggtgcatg agtacgggga aaattttctc
    32041 atgtacactc gtattctaac ggatacagcc tttgtctact tgccagcttt ggtggcttgg
    32101 tcagccttta gggtctttgg gggtaatcct attatcggta ttgttttagg tttgatgctg
    32161 gtgtccaatg aattgcctaa tgcttgggta gttgcctctg gtggagatgt taagccgcta
    32221 accttctttg gatttgttcc tgttgttggt tatcaaggta ccgttttacc tgccttcttt
    32281 gttggtctgg taggagctaa gttggagaaa tggttgcaca aaaaggttcc agaagcttta
    32341 gacttattgg tcacaccgtt tttaacgttt gctattatga gtaccttggg actatttgtg
    32401 attggaccag ttttccattc tcttgaaaac cttgttctgg ctgggacaca ggccgtcttg
    32461 catttgccgt ttggcattgc tggtcttatt gttggtggta tccaacagtt gatcgtcgtt
    32521 actggtattc accatatctt taacttccta gaagcgcagc taattgccaa taccggaaaa
    32581 gatcctttca atgcttacct aacagcagca acagctgctc aagctggagc taccttagct
    32641 gttgctgtta aaaccaaatc aacaaaacta aaaggtctgg cctttccatc aaccttgtca
    32701 gctcttttag ggattactga accagctatt tttggggtca acctccgtta tccaaaagtc
    32761 tttgtttcag gtcttattgg tggtgcctta ggtggttggg tcgctgggct ctttggcatt
    32821 gcaggaactg gttttgggat tacggtctta ccaggaactc tcctttactt gaatggccat
    32881 ttattacaat accttgtgac tatgcttgtc ggtcttgggg tagcctttgg aattgcttat
    32941 acttggggtt atcaagatag agaaaccctt ccgttaccag ctgtcgaagt tgatcaaacg
    33001 gctgatcagc cagccttagc agaggaaact ttatatagtc ctttgaatgg aacagttgta
    33061 gatttagcag ctgtttcaga ccctgttttt tcatcaggtg ctatgggtca aggcttagca
    33121 attaagccag aagataatac cctatactca ccagttgatg gtaaagttga aattgtcttt
    33181 gaaacaggtc atgcctatgc tataacgtca agtcaaggag cagaagtgtt acttcatatt
    33241 ggtattgaca ctgtgtcgat ggcaggagat ggctttgaat ctcttgtagc agtaggtcag
    33301 gcggttaaaa aaggtgatct tcttggacac tttgatccta gcaagattgc agaagctgga
    33361 ctagatgaca ccaccatgat gattgtgacc aatagcgctg actatcaaag cgtggacatt
    33421 cttgctcaag gacatgtttt gattggtgat caagtggcac ttatcaaata aggacaaaag
    33481 agctaactaa gcagctcttt tgtcctgcca ttcatggcag gttggcaaaa aaatgagaaa
    33541 agcctaaaaa ccttaaattt gtgttatttt acctatactt tgtaccttgt tttttttaat
    33601 aaagtgttgt tatacttaag gcgaactata ggaatgcgct tacatagatg gtatatcaac
    33661 tgggaagcca tgacttagta ccaaaaataa ggagtgtcca aatggataaa catttgttgg
    33721 taaaaagaac actagggtgt gtttgtgctg caacgttgat gggagctgcc ttagcgaccc
    33781 accatgattc actcaatact gtaaaagcgg aggagaagac tgttcaggtt cagaaagaat
    33841 tatcttctat agatagcttg cattatctgt cagagaatag caaaaaagaa tttaaagaag
    33901 aactctcaaa agcggggcaa gaatctcaaa aggtcaaaga gatattagca aaagctcagc
    33961 aggcagataa acaagctcaa gaacttgcca aaatgaaaat tcctgagaaa ataccgatga
    34021 aaccgttaca tggtcctctc tacggtggtt actttagaac ttggcatgac aaaacatcag
    34081 atccaacaga aaaagacaaa gttaactcga tgggagagct tcctaaagaa gtagatctag
    34141 cctttatttt ccacgattgg acaaaagatt atagcctttt ttggaaagaa ttggccacca
    34201 aacatgtgcc aaagttaaac aagcaaggga cacgtgtcat tcgtaccatt ccatggcgtt
    34261 tcctagctgg aggtgataac agtggtattg cagaagatac cagtaaatac ccaaatacac
    34321 cagagggaaa taaagcttta gccaaagcta ttgttgatga atatgtttat aaatacaacc
    34381 ttgatggctt agatgtggat gttgaacatg atagtatccc gaaagtaaat ggagaagcta
    34441 gtgacgaaaa tctcaagcga tctattgatg tttttgaaga gattgggaaa ttaattggac
    34501 caaaaggtgc tgataaatcg cgtctgttta ttatggatag cacctacatg gctgataaaa
    34561 acccattgat tgagcgagga gctccttata ttgatttgtt attggtacaa gtctatggcg
    34621 caagaggaga acaaggtgag tttcaaaacg ataccaagtt agttacagat acccctgaag
    34681 aacgatggca aggttatagc aagtatattc gtcctgaaca atacatgatt ggtttttctt
    34741 tctatgagga aagagcaggc agcggcaatc tttggtatga tattaattct cgcaaggacg
    34801 aggacaaagc aaatggaatt aacactgaca taactggaac gcgtgccgaa cggtatgcaa
    34861 ggtggcaacc taaaacaggt ggggttaagg gaggaatctt ctcctatgct gtggatcgtg
    34921 atggtgtagc tcatcaaccc aaacaaatag ctgaaaaaga taaacagaat gtaaaaaata
    34981 atcaacctca gattccagag attactgata acattttcca ctcagattat agtgtctcca
    35041 aggcactaaa gacagttatg ctaaaagata agtcgtatga tctgattgat gagaaagatt
    35101 tcccagataa ggctttgcga gaagctgtga tggcgcaggt tggaaccaga aaaggtgatt
    35161 tggaacgttt caatggcata ttacgattgg ataatccagc gattcaaagt ttagaaggtc
    35221 taaataaatt taaaaaatta gctcaattag acttgattgg cttatctcgc attacaaagc
    35281 tcgaccaatc tgttttaccc gctaatatga agccaggcaa agataccttg gaaacagttc
    35341 ttgaaaccta taaaaaggat aacaaagaag aacctgctac tatcccacca gtatctttga
    35401 aggtttctgg tttaactggt ctgaaagaat tagatttgtc aggttttgac cgtgaaacct
    35461 tggctggtct tgatgtcgct actctaacgt ctttagaaaa agttgatatt tctggcaaca
    35521 aacttgattt ggctccagga acagaaaatc gacaaatttt tgatactatg ctatcaactg
    35581 tcagcaatca tgttggaagc aatgaacaaa cagtgaaatt tgacaagcaa aaaccaactg
    35641 ggcattaccc agatacctat ggcaaaacta gtctgcgctt accagtggca aatggaaaag
    35701 ttgatttgca aagccagctt ttgtttggga ctgtgacgaa tcaaggaacc ctaatcaata
    35761 gcgaagcaga ctataaggct taccaaaatc agcagattgc tggacgtagc tttgttgatt
    35821 caaactatca ttacaataac tttaaagttt cttatgagaa ctataccgtt aaagtaactg
    35881 attccacatt gggaaccact actgacaaaa cgctagcgac tgataaagaa gagacctata
    35941 aggttgactt ctttagccca gcagataaga caaaagctgt tcatactgct aaagtgattg
    36001 ttggtgacga aaaaaccatg atggttaatt tggcagaagg cgcaacagtt attggaggaa
    36061 gcgctgatcc tgtaaatgca agaaaggtat ttgatgggca actgggcagt gagactgata
    36121 atatctcttt aggatgggat tctaagcaaa gtattatatt taaattgaaa gaagatggat
    36181 taataaagta ttggcgtttc ttcaatgatt cagcccgaaa tcctaagaca accaataaac
    36241 ctattcagga agcaagtctg caaattttta atatcaaaga ttataatcta gataatttgt
    36301 tggaaaatcc caataaattt gatgatgaaa aatattggat tactgtagat acttacagtg
    36361 cacaaggaga gagagctact gcattcagta atacattaaa taatattact agtaaatatt
    36421 ggcgagttgt ctttgatact aaaggagata gatatagttc gccagtagtc cctgaactcc
    36481 aaattttagg ttatccgtta cctaacgccg acactatcat gaaaacagta actattgcta
    36541 aaggtctatc tcaacaaaaa gataagtttt ctcaaaagat gcttgatgag ttaaaaataa
    36601 aagagatggc tttagaaact tctttgaaca gtaagatttt tgatgtaact gctattaatg
    36661 ctaatgctgg agttttgaaa gattgtattg aaaaaaggca gctgctaaaa aaataaacaa
    36721 agcaactttc ttagatagca acattcagat taaattaaca aaatgtgact atgataaagg
    36781 tttgctggaa ttgattaacc aaaagactaa aaatctgaga tgaatagtcc cagattttta
    36841 gtcttttata ggttttgatg acataaagct aaataatcgt tagactacca gaaagtggca
    36901 cttgtccgtg agacatggct gtcttgataa gagcctgtgt catcataaag ataaaaaaga
    36961 acaagaaaaa ggagaaaaca tgggaaaatt atatggaagc attgaagctg gtgggacaaa
    37021 gtttgtctgt gccgtgggtg atgaggagtt tacagtggtg gataaaacac aatttctgac
    37081 caccacacca gaagaaacca tcgcgcgaac catcgcttat tttaaagctt ttgaggcaga
    37141 tttggctgga atggcgattg gttcttttgg accaattgat attgatccgt cgtcagagac
    37201 ttacggtcat atcacaacca ctcctaagtc aggctgggct aatgtggatt tgttaggaca
    37261 actttcggct gcttttaaga ttccttttga tgtgacaaca gatgttaata gctctgctta
    37321 tggtgaggtg cttgctcgtc ctggtgtgga aagtttggtc tattacacca taggtactgg
    37381 tattggggcg ggagctattc aacatggcca ctttattggg ggattaggtc atacagaagc
    37441 gggtcatacc tatgtcatgc cccatcctga tgatatggcc aagggatttt taggtgtttg
    37501 cccttttcat aaagggtgtc tggaagggat ggcagcaggt ccaagtattg aggccagaac
    37561 gggagtgaga ggtgaacggc ttgatcagga agctgatgtt tgggacattc aagccttcta
    37621 cattgcccaa gcagcgctgc aagcaactat gctttaccgt cctcaagtga ttgtttttgg
    37681 cggaggtgtc atgacgcaag aacatatggt gttgcgcgtg catgataaat tcactaccct
    37741 tttaagcggt tatttgccag tccctgattt gacagactac attgttactc cagctgttgc
    37801 tgataatggc tccgctactc tgggaaattt tgctttggcc aagttggctg cacaaggaaa
    37861 atagggtctg tttttgcctg cttgtctagt aaaagaaagc gcagtcagct agaacatgct
    37921 ataataaaca gagattaaca tcagaagcga ggaatattat gtcagaacca ttatttctaa
    37981 aatcaaccat gcacgacaga atttggggtg gcaccaagtt gagagatgtc tttgcttaca
    38041 acatcccaag tgacaccaca ggtgagtatt gggctatttc tgctcatcct aatggtgttt
    38101 cgacagttac taatggacgt tatcaaggac aacctttaaa tacattatat gcgcaagagc
    38161 cggccttgtt tgggaatcca aaggaagaag tttttccact gttaactaag attttagatg
    38221 ctaatgactg gctaagtgtc caagttcatc ctgatgatgc ttatggacgt gaacatgaag
    38281 gagaattagg caagacagaa tgctggtata ttatttctgc cgaagaaggt tctgaaattg
    38341 tatacggcca tcaggccaag tccaaagaag accttcgtgc catgattgaa gcaggcgctt
    38401 gggatgattt gctgactcgt gtcccagtca aagcaggcga ttttttctat gttccaagtg
    38461 gcaccatgca tgcgatcggc aagggaattc ttattttaga aacccagcag tcatctgata
    38521 ccacttatcg ggtttatgat tttgaccgta aggacgttaa tggtaatctt cgtgaccttc
    38581 atattgaaaa gtctattgat gtcttaacga ttggtaagcc agaaaatagc gtgccagcca
    38641 ctatagtttt ggataatatg gtagaaacaa ctttagtctc aacgccattt ttcactgttt
    38701 acaaatgggt aactagtcaa atggttgaca tgaaacaagc tgcgccttat cttttagtga
    38761 gtgttttaaa aggtcaagga aaactctatg tggaccagaa ggcttatgag ctagaaaaag
    38821 gcatgcactt tatcttgcct aacgatgtta aatcgtggtc atttgatggt caattagaaa
    38881 tgattgtgag tcaccccaat caactcgttt aataccatgg tatcaatgtg agaaaaaagg
    38941 ataaaattat gactctctgt ttctctgata actttaccaa cctttcttga actgcctaca
    39001 tatttatgtt aaaatgaaat gattagatga tcgaaaggaa agagaatggc caatattcta
    39061 cgcaaagtta tcgaaaatga caaaggcgaa ctaagaaaat tagaaaaaat tgcaaaaaaa
    39121 gtagaatcct acgcagacca aatggtgtca ttgtcggata gagatctaca aggaaaaaca
    39181 cttgaattta aagaacgcta tcaaaaaggt gagaccctcg aacaactatt acctgaggct
    39241 ttcgcagttg ttcgtgaagc agcaaaacgt gtgttgggct tgttccctta tcgtgttcaa
    39301 attatgggag gaattgttct tcataacggg gatgtccctg aaatgcgtac tggtgaagga
    39361 aaaactctta cagccaccat gccagtttat ctcaatgcta ttgctggtga gggtgttcac
    39421 gttattactg ttaatgagta tctttcaacg cgtgacgcta ccgaaatggg tgaagtgtat
    39481 agctggttag gtctttcagt tgggatcaac ctagctgcta agtctcctgc tgaaaaacgt
    39541 gaagcttata attgtgatat tacttattca accaactctg aagtcggttt tgattacctt
    39601 cgtgacaaca tggttgttcg ccaggaagac atggtacaac gcccactcaa ttttgccttg
    39661 gttgatgaag tggactcagt cttaattgac gaggcaagga cacctttaat tgtctctggt
    39721 gctgttagct ctgaaaccaa tcaattgtat attcgtgctg acatgtttgt aaaaactctg
    39781 acgtcagtgg attatgttat tgatgtgccc actaaaacga ttggattaag cgattcaggt
    39841 attgataaag ctgagagtta ttttaacttg agcaatcttt acgatattga aaatgtagcc
    39901 ttgactcact ttatagacaa cgcccttcgt gcaaactata ttatgttgtt agatatcgat
    39961 tatgtggtca gcgaggatgg tgagattctt attgttgacc aatttacagg tcgtacgatg
    40021 gaagggcgtc gcttctcaga cggcttgcac caagctattg aggcaaaaga aggcgttcgt
    40081 attcaggaag aatcaaaaac cagcgcctca atcacctacc aaaatatgtt tcgtatgtac
    40141 aaaaaattgg caggtatgac tggtactgcg aagaccgagg aagaagaatt tcgcgaagtt
    40201 tataacatgc gtattattcc aattccaacc aatcgtccaa ttgcccgtat tgatcacact
    40261 gatttgcttt acccgacctt agaatcaaag tttagagcag ttgtggaaga cgttaaaacg
    40321 cgtcatgcaa aaggacaacc tatccttgta ggtacggttg ctgttgaaac tagtgatttg
    40381 atttcacgca agctggttga agcagggatt cctcatgagg ttttgaatgc taaaaatcac
    40441 tttaaagaag ctcaaatcat tatgaatgct gggcaacgtg gtgctgttac aatcgcaact
    40501 aacatggctg gtcgtggtac tgatatcaag cttggtgaag gtgttcgtga acttggtggg
    40561 ttatgtgtca ttggtacaga gcgtcatgaa agccgtcgta tcgataacca gcttcgtgga
    40621 cgttcaggtc gtcaaggaga tccaggtgaa tcccaattct atctttcatt agaagatgat
    40681 ttgatgagac gtttcggctc tgatcgtata aaagcctttt tggatcggat gaaattagat
    40741 gaagaagaca ctgttattaa atcaggtatg ttagggcgtc aagtggaatc tgctcaaaaa
    40801 cgggttgaag gaaataacta cgatacccgt aaacaggtct tacaatatga tgatgtgatg
    40861 cgtgaacagc gtgaaattat ctatgctaac cgtcgtgatg tcataactgc taatcgagat
    40921 cttggcccag aaattaaagc tatgatcaag cgtactatcg accgtgctgt agatgctcat
    40981 gcacgtagca atcgtaaaga tgctatagat gctattgtaa cttttgctag gacctcgctg
    41041 gtaccagaag agtctattag tgctaaagag ttacgtggtt taaaagatga tcaaatcaaa
    41101 gagaagcttt atcaaagagc gcttgctatc tacgaccagc aactatcaaa acttcgtgat
    41161 caagaagcta ttattgaatt ccaaaaagta ctcattttga tgattgtgga taacaaatgg
    41221 actgaacaca ttgatgcctt agatcaactt cgtaatgctg ttggacttcg ggggtatgct
    41281 caaaataacc ctgtggtgga ataccaagca gaaggtttca agatgttcca agatatgatt
    41341 ggtgctatcg agtttgatgt aacacgtacc atgatgaaag cacaaattca cgaacaagaa
    41401 cgtgaacgtg caagtcaacg tgccacaaca gcagcacctc aaaacatcca atcgcagcag
    41461 tcagctaaca ccgatgattt acccaaagtg gagcgtaacg aagcttgtcc atgtggttca
    41521 ggcaaaaaat tcaaaaattg tcacggtcgt aaatccttct cataagaacg agacacaaca
    41581 ggacaaagtt aggtatatag aaagaagctg agaataattc tcagcttctc actgtttaaa
    41641 tcttctaaca ttaagatgaa ataaggtttg gtggaaagta atgattgttg gacatggaat
    41701 agacttacaa gagatttcag ctattgaaaa agtttatcaa agaaatcctc gctttgctca
    41761 aaaaatttta actgagcagg aattggctat ttttgagagt tttccttata aacgtcgact
    41821 cagctatttg gcgggtcgtt gggcaggcaa agaggctttt gctaaggcta tagggacagg
    41881 tattgggcgg ttaacctttc aagatattga gattttaaat gatgttagag gatgtcccat
    41941 tctgacaaaa tctccgttta aaggaaatag ttttattagc atctcacata gtggcaatta
    42001 tgtacaggct agtgttattt tggaggataa aaaatgattt caagtttcca tcgcccaaca
    42061 gttgcaaggg tcaacttaca agctattaag gagaatgttg ccagtgttca aaagcatatt
    42121 ccactagggg taaaaacgta tgcagttgtc aaggctgatg cttatggtca tggtgctgtc
    42181 caggtgtcaa aagcactcct acctcaagtg gatgggtact gtgtgtcaaa tcttgatgag
    42241 gctttgcaat tacgtcaagc aggcattgat aaagagattt taattcttgg agtcctcctg
    42301 ccaaatgaat tagagttagc agttgctaat gctattactg ttacaatcgc tagtttagac
    42361 tggatagctt tagctagact ggagaaaaaa gaatgtcaag gcttaaaagt tcatgtaaaa
    42421 gttgattctg gtatggggcg gatcgggctt cgttcttcaa aagcagtcaa tttattgatt
    42481 gatagtctaa aagagttggg tgctgatgta gaaggtattt tcactcattt tgccacagct
    42541 gatgaggcag atgatactaa atttaaccag cagttacagt tttttaaaaa gctgatagct
    42601 ggacttgagg ataagcctcg tttagtacat gctagtaatt cagccacaag tatctggcat
    42661 agtgatacca tttttaatgc tgttcgttta ggaattgtta gttatggttt gaatccaagt
    42721 ggttctgatc taagcttacc atttccactg caagaggctt tatctctaga atctagctta
    42781 gtgcatgtca agatgatttc agctggtgat acagtcggtt atggagctac ttatactgcc
    42841 aaaaagtctg aatatgtagg gaccgtccca atcggttatg cagatggctg gaccaggaac
    42901 atgcaaggct tttcggtgtt agttgatgga caattctgtg aaattatagg gcgtgtatcg
    42961 atggatcaac tgaccatacg acttcccaaa gcatatcctt taggaacaaa agtcactttg
    43021 attggcagca atcagcaaaa aaatatttct acaacagata tcgcaaatta ccgtaataca
    43081 atcaattatg aagttctatg ccttttaagt gaccgtattc ctcggatata ttaagaaaat
    43141 cacgtaaaaa taattgcatt gtgcttatct ttaatgttat aataaagtga ggacattaga
    43201 aagaagtgac taattaatat atgaataaaa acaaactatt aagagttgcc atgctactaa
    43261 gtctcttagc cccgacagca gaaagcatga cagtgttggc tcaagatgta atgcttgaga
    43321 cgcataaagc aactacaaat gaaaccagtg attcttcttc aaaagaggaa aataataaaa
    43381 atgcagcacc tacaacatca gataaaactg accaaggtcc ccttgatgct tctgcagaaa
    43441 caaactctaa tagtcttgtt aacgcggatg ataaaaaaag aagcgattct agtcagtctg
    43501 ctgtaggctc ttcgaacaac aaggcagaag cagaaaacca ggtagatgat aaatcaactg
    43561 atcattcgaa accaactgac cagccaaaac cgactgatca gcccaaacca tcaccatcta
    43621 aagttgatac ggcacctgct tcttcattgt cgagacagct gccagaggca agaactccta
    43681 ttcagtcgtt gtccccttac gtatcagatt tagatttgag tgagatagat atcccttctg
    43741 tcaacacata cgcggcatat gtagagcatt ggagtggtaa aaatgcctat acccatcatc
    43801 ttttatctcg ccgttatggt attaaagctg accagattga tagttactta aaatcaacag
    43861 gcattgccta tgacagcaca cgtattaatg gtgagaagct attgcaatgg gaaaagaaaa
    43921 gtgggctgga tgttcgagct atcgtagcta ttgcgatgtc tgagagttct ttaggaactc
    43981 aagggattgc gactttgctt ggagctaata tgtttggcta tgcagctttt gatctagatc
    44041 cgactcaagc aagtaagttt aatgatgata gtgctattgt caaaatgaca caagatacca
    44101 ttattaaaaa caaaaatagc aattttgcac ttcaagattt aaaagcggct aagttttcac
    44161 gaggtcaatt aaactttgca agtgacgggg gtgtttattt tactgatact actggtagtg
    44221 gtaaacgtcg cgcacaaatt atggaagacc tggataagtg gattgatgac catggtggca
    44281 caccagccat tccagccgaa ttgaaagtgc agtcatcagc tagttttgca tctgtgccag
    44341 caggttataa gctctctaag agttatgatg tcttgggtta tcaagcttcg agttatgctt
    44401 ggggacaatg cacttggtat gtgtataatc gcgccaaaga attgggttac caatttgatc
    44461 cttttatggg aaatggtgga gattggaagt ataaagcagg gtatgccctg tcaaagactc
    44521 caaaagtagg ttatgctatt tcatttgcac cagggcaagc aggcgctgat ggcacttatg
    44581 gccacgtatc aattgtagaa gatgttagaa aagatgggtc tattcttatt tcagagtcta
    44641 actgtatcgg cttaggtaag atttcttatc gtacctttac agctcagcag gctgaacagc
    44701 taacatatgt tattggcaag agtaaaaact aataactaat gacttttctt aaggcttaat
    44761 tttcttagcg ggagttaggc cttttaaact gttggtgtaa cacgataaag tttcaagtaa
    44821 ataaatgatt ttgagacaat agcacgatta gggctattgt ctttttttat tgtgatgaat
    44881 atgcagtagt gacatctcat gttgtctcct aacacaatta tggagcttat aacgtgttac
    44941 atgttttgtg aatgaagtaa tgattgaatc agccctagta ataactatcc taaggctagc
    45001 tttatctcag taatattgtt gtagaggaat tcttttatca gagatatcat cattatcaat
    45061 ccaggcttat tgctagtgtc ttggctcaca tagtggagct ttttaacaga caattggacc
    45121 attggaaaca atcttgacaa aataatcaca aagtgataga ttaagttcag ttaataaaaa
    45181 agttagctta ttcataaaaa tttttagaaa gttgattgaa aatgaaaaaa atcagcaaat
    45241 gtgcgtttgt ggcaatatct gcccttgttc tcattcaggc tactcaaact gtaaaatcac
    45301 aagagccttt agttcagtca caactcgtga caacagtagc tttaactcag gataatcgac
    45361 ttttagttga agagataggc ccttacgcta gtcaatcagc tggaaaagag tattataaac
    45421 atattgaaaa ggttattgtt gataatgatg tctatgaaaa aagcctggaa ggcgagcgaa
    45481 cctttgatat taactaccaa gggattaaga tcaatgctaa ccttattaaa gacggtaagc
    45541 atgaattgac tattgttaat aaaaaagatg gtgatatcct aattaccttt attaaaaagg
    45601 gcgataaagt gacctttatt tcagctcaaa aattaggaac aacagatcat caggattcat
    45661 taaaaaaaga tgtgctcagt gataaaacag tgccacaaaa ccaaggcaca caaaaagttg
    45721 ttaaatctgg gaaaaatact gctaacttgt cattaataac aaaattgagt caagaagatg
    45781 gtgcaatttt atttccagaa attgatcgtt attctgataa caaacagata aaagcattga
    45841 ctcagcaaat cacaaaggtt acagtcaatg gtacagttta taaagatctt atttcagatt
    45901 ctgtaaaaga tactaatggc tgggtctcga atatgacagg gcttcatctt ggaacaaaag
    45961 ctttcaaaga tggagaaaat acaatcgtga tatcctcaaa aggatttgaa gacgttacca
    46021 ttaccgttac caagaaagat ggtcaaatcc attttgtatc tgccaaacaa aaacaacatg
    46081 tgactgctga agacagacaa tcaacaaagt tggatgtcac cactttgaaa aaagctatca
    46141 aagaagcgga tgcgattatt gctaaagaaa gcaacaaaga cgcggtcaaa gatctggctg
    46201 agaaacttca agtcatcaag gattcttaca aagaaatcaa agatagtaag ctactcgccg
    46261 atactcatcg actgttaaaa gataccatcg agtcttatca agcaggtgag gtttctatta
    46321 acaatctcac agaaggaacc tatacgctaa actttaaagc taataaagaa aactcagaag
    46381 agtcctccat gcttcaaggt gcttttgata aaagagccaa attagtggtt aaagcagatg
    46441 gtacaatgga aatttccatg cttaatactg ctttaggaca atttttgatt gacttttcta
    46501 ttgaaagcaa agggacctac ccagcagcag tgcgtaaaca agttggccaa aaagatatca
    46561 atggtagcta tattcgaagc gaatttacca tgcctattga tgatttggat aaattacaca
    46621 aaggtgctgt tttggtatca gccatgggag gtcaagaaag tgatttaaac cactatgaca
    46681 aatacaccaa acttgacatg acctttagta agaccgttac caaaggctgg agtggttatc
    46741 aggtagaaac tgatgataaa gaaaaagggg ttgggactga acgtcttgaa aaagttttag
    46801 ttaaacttgg caaagattta gacggcgatg gtaaattatc aaaaacggaa ttagaacaga
    46861 ttcgaggcga gttgcgtcta gaccattacg agttaactga tatttcttta ttgaaacatg
    46921 ctaaaaatat tacagaacta catctggatg gaaaccaaat tacggaaatt ccaaaagagt
    46981 tatttagtca aatgaagcaa cttcgatttc ttaacttaag aagtaatcat ttaacttatc
    47041 tagacaaaga tacatttaaa agcaacgctc aattaagaga actctactta tcaagtaact
    47101 ttattcactc tcttgaagga ggactattcc agtcgcttca tcacctggag caacttgatc
    47161 tttccaagaa tcgtattggc cgactttgtg ataacccatt tgaaggattg tctcgcctga
    47221 cttcattagg tttcgcagaa aatagtcttg aggagatacc tgaaaaagcg ctagagcctc
    47281 taacatcact taactttatc gacttatctc aaaataattt agcactactg ccaaaaacaa
    47341 tagaaaaatt gcgcgcctta agcactattg tggcaagtag aaatcatatt actcgtattg
    47401 ataatatttc atttaaaaat cttcctaaat tatctgtact cgatttatca actaatgaaa
    47461 tttc
//
LOCUS       DATUYR010000007        47283 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.007, whole genome shotgun sequence.
ACCESSION   DATUYR010000007 DATUYR010000000
VERSION     DATUYR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47283
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.007"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            168..962
                     /locus_tag="VCI33_000336"
     CDS             168..962
                     /locus_tag="VCI33_000336"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEP2831290.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGVAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            987..1628
                     /locus_tag="VCI33_000337"
     CDS             987..1628
                     /locus_tag="VCI33_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEP2831291.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVAYLKSVLEQ"
     gene            1657..2658
                     /locus_tag="VCI33_000338"
     CDS             1657..2658
                     /locus_tag="VCI33_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEP2831292.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEANIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMSLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEILAREARKRQV
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADTTMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLHNQRDVQR"
     gene            2663..3298
                     /locus_tag="VCI33_000339"
     CDS             2663..3298
                     /locus_tag="VCI33_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEP2831293.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHGYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGISFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            3594..4244
                     /locus_tag="VCI33_000340"
     CDS             3594..4244
                     /locus_tag="VCI33_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEP2831294.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSEKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            4941..6065
                     /gene="queG"
                     /locus_tag="VCI33_000341"
     CDS             4941..6065
                     /gene="queG"
                     /locus_tag="VCI33_000341"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEP2831295.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVMEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGGWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEMLAFMSHLTLKDEDSQKELELIRRKWQV"
     gene            6134..7232
                     /gene="prfB"
                     /locus_tag="VCI33_000342"
     CDS             join(6134..6205,6207..7232)
                     /gene="prfB"
                     /locus_tag="VCI33_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEP2831296.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            7251..7943
                     /gene="ftsE"
                     /locus_tag="VCI33_000343"
     CDS             7251..7943
                     /gene="ftsE"
                     /locus_tag="VCI33_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEP2831297.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            7936..8865
                     /locus_tag="VCI33_000344"
     CDS             7936..8865
                     /locus_tag="VCI33_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831298.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(9175..9873)
                     /locus_tag="VCI33_000345"
     CDS             complement(9175..9873)
                     /locus_tag="VCI33_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP2831299.1"
                     /translation="MPFIFRHSFFNKALIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            10041..10805
                     /locus_tag="VCI33_000346"
     CDS             10041..10805
                     /locus_tag="VCI33_000346"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEP2831300.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDTVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLVGDDSNYITGQTIVVDGGMVFH"
     gene            10913..13414
                     /locus_tag="VCI33_000347"
     CDS             10913..13414
                     /locus_tag="VCI33_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEP2831301.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLSHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLAYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFTR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            13749..14942
                     /locus_tag="VCI33_000348"
     CDS             13749..14942
                     /locus_tag="VCI33_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP2831302.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            14972..16318
                     /gene="asnS"
                     /locus_tag="VCI33_000349"
     CDS             14972..16318
                     /gene="asnS"
                     /locus_tag="VCI33_000349"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEP2831303.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            16733..17623
                     /gene="rapZ"
                     /locus_tag="VCI33_000350"
     CDS             16733..17623
                     /gene="rapZ"
                     /locus_tag="VCI33_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEP2831304.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            17620..18597
                     /locus_tag="VCI33_000351"
     CDS             17620..18597
                     /locus_tag="VCI33_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEP2831305.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLTPPGDLRNVLLAMSDMPKFYERVFQYRFNESDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            18594..19505
                     /gene="whiA"
                     /locus_tag="VCI33_000352"
     CDS             18594..19505
                     /gene="whiA"
                     /locus_tag="VCI33_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEP2831306.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYVQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            19638..21035
                     /locus_tag="VCI33_000353"
     CDS             19638..21035
                     /locus_tag="VCI33_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP2831307.1"
                     /translation="MACTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMIVVKPEDQ
                     PRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATETITTNSR
                     VLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVAFSDEHDI
                     WWLETIGGHHWIARRVPDDAYVTNPNQFGIDYFEFNNPEDYLCSADLKDFIDTYHLDL
                     TYNHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWCQKPYRKI
                     TVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRPNKPQEIA
                     AIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAALADPHYNH
                     HETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQTLLNKIL
                     FDASNLMTNRFSLSD"
     gene            21187..22734
                     /locus_tag="VCI33_000354"
     CDS             21187..22734
                     /locus_tag="VCI33_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEP2831308.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADVFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGVGHDHHHEEADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            22882..23604
                     /locus_tag="VCI33_000355"
     CDS             22882..23604
                     /locus_tag="VCI33_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2831309.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKVGKTPKTEILS
                     FEQYQVTPYLSGLLEFDLDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            23624..24823
                     /locus_tag="VCI33_000356"
     CDS             23624..24823
                     /locus_tag="VCI33_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEP2831310.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(24920..25180)
                     /locus_tag="VCI33_000357"
     CDS             complement(24920..25180)
                     /locus_tag="VCI33_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEP2831311.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(25295..26236)
                     /locus_tag="VCI33_000358"
     CDS             complement(25295..26236)
                     /locus_tag="VCI33_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEP2831312.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLTWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            26630..27079
                     /locus_tag="VCI33_000359"
     CDS             26630..27079
                     /locus_tag="VCI33_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEP2831313.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSDQFALTGAVKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            27254..27538
                     /locus_tag="VCI33_000360"
     CDS             27254..27538
                     /locus_tag="VCI33_000360"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEP2831314.1"
                     /translation="MRLEEIRQEINGIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLTGGDIND"
     gene            27531..28793
                     /locus_tag="VCI33_000361"
     CDS             27531..28793
                     /locus_tag="VCI33_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEP2831315.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGATVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFAYLLGWFKPYLSQKLPNPYFRIAFIGALLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            28908..29255
                     /gene="rplS"
                     /locus_tag="VCI33_000362"
     CDS             28908..29255
                     /gene="rplS"
                     /locus_tag="VCI33_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEP2831316.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            29502..29573
                     /locus_tag="VCI33_000363"
     tRNA            29502..29573
                     /locus_tag="VCI33_000363"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29535..29537,aa:Arg,seq:cct)
     gene            complement(29616..29873)
                     /locus_tag="VCI33_000364"
                     /pseudo
     CDS             complement(29616..29873)
                     /locus_tag="VCI33_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            30269..30838
                     /locus_tag="VCI33_000365"
     CDS             30269..30838
                     /locus_tag="VCI33_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEP2831317.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVAMFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            30839..32791
                     /gene="gyrB"
                     /locus_tag="VCI33_000366"
     CDS             30839..32791
                     /gene="gyrB"
                     /locus_tag="VCI33_000366"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEP2831318.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYNVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRNFIEENAVYSTLDI"
     gene            33159..34883
                     /gene="ezrA"
                     /locus_tag="VCI33_000367"
     CDS             33159..34883
                     /gene="ezrA"
                     /locus_tag="VCI33_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEP2831319.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(35015..35473)
                     /locus_tag="VCI33_000368"
     CDS             complement(35015..35473)
                     /locus_tag="VCI33_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEP2831320.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQPPEKTKSFWKRFLKK"
     gene            35700..37007
                     /gene="eno"
                     /locus_tag="VCI33_000369"
     CDS             35700..37007
                     /gene="eno"
                     /locus_tag="VCI33_000369"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEP2831321.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(37489..38517)
                     /locus_tag="VCI33_000370"
                     /pseudo
     CDS             complement(37489..38517)
                     /locus_tag="VCI33_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(38797..40338)
                     /locus_tag="VCI33_000371"
     CDS             complement(38797..40338)
                     /locus_tag="VCI33_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2831322.1"
                     /translation="MLHLHLETKLQDKLSLLNILLDVSEVSIDQLCQETELKKQRVYN
                     LLFEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLYQTSIFLKMLCFLIEPGEL
                     SLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNHIVGPEYRIRFLIALLHYQFG
                     MTIYDFDKTSMNKVVSLIINSNQAITLNDASKAPYEFSYFAILISLIWKRRHDNLGIP
                     QTDAFKHLKKLSIYRDIKMTSQEIIGKWYHPDLTDEDLDYIFLCFCTTNNSFHKDKWT
                     PKKVKELFELVMTKTNGKTLKASLRPLLGNNILNSLPFKRILVSFSRLFISNLQVLLP
                     DIHLFHYLRRQQKRNKSFYNTLKTIVEEWMSAEGIVGKLPSYHLLLFTIQLEELLKTY
                     LPPIPVYLLTNNTAALDLMTNALSIYFPPAIATVMPVNVEIIPFKDIVKEKQSVIIAD
                     RQYLNLIQHLYQNQGHLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDEFIVTLLDTYHK
                     NLSSP"
     gene            41694..46718
                     /locus_tag="VCI33_000372"
     CDS             41694..46718
                     /locus_tag="VCI33_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP2831323.1"
                     /translation="MLKKIEVKEGQKNPISITLSEDQAKSLKNKEKLKVSIKQKQSKK
                     TSKDFFFEVGIDPKVKAKQQEKLLELDKVKKQIEDSINGDAWLPEKSEGEKPVQNTNK
                     ELQLQELNKKYQMAKEAIESATTLDDVETQFDKYTKVGDKDKYPDSLRNQYTRGDKDK
                     EIEKAKKSLGDLSDKVNGKIEEDKWLSDEVKKKQQQELEARKQKVNDSLKGSDSLKSL
                     RETVEKASSKNQKKPESFEDVYVPGNEETEKTKVRDILQKTYQKTEQNIETDPWLSPE
                     QKKAQKENAKARLDAGLKAVETTESLDKLKEVESDFLDKEKNPDSIPNQHKAGTPEVA
                     RKTFLDNFEKEAKKEIESIDKDDTLTANAKQVAKDKVAQQLQEATAKVEKAQSFDDLK
                     NIQDKFNSELPHTPGKPLKDQQSDAIAELEKKQQEIEKAIEGDKTLPRDEKEKQIADS
                     KERLKSDTQKVKDAKNADAIKKAFEEGKVDIPQAHIPGDLNKDKEKLLAELKQKADDT
                     EKAIDSDKTLTAKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIEDTHV
                     KGNLEGIKNKAIEDLKKAHTETVAKINGDDTLDKATKEAQVKEADKALAAGKEAITKA
                     DDADKVSTAVTEHTPKIKAAHKTGDLKKAQVDANTALDKAAEKEREEINNDITLTAKD
                     KEQQLKEVETALTKAKDNVKAAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVA
                     KLEEAAKATKDKISADPTLTSKEKEEQSKAVNAELKKAIEAVNAADTADKVDDALGKG
                     VTDIKNQHKTGDPVVARREAHGKQLDRVAQETKDAIEKDPTLTTEEKAKQVKDVDAAK
                     ERGMAKLTEAKNADELDKAYGEGVTDIKNQHKSGDPIEARRGLHNKSIDEVAQATKDA
                     ITADTTLTEAEKETQRGNVDKEATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGK
                     GLDVRKDEHKKALEAVAKRVTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKD
                     ADEADKAYGDGVTAIENAHVIGKGIEARKDLAKKDLAEAAAKTKALIIEDKTLTDDQR
                     KEQLLGVDTEYAKGIENIDAAKDAAGVDKAYSDGVRDILAQYKEGQNLNDRRNAAKEF
                     LLKEADKVTKLINDDPTLTHDQKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGI
                     ENINNQYQHGDGVDVRKATAKDDLEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKN
                     TAIAAVDKATTADGVNQELGKGITAINKAYRPGEAVKARKEAAKADLEKEAAKVKALI
                     AKDPTLTQAAKDKQTAAVDAAKNTAIVAVDKATTAEGINQELGKGITAINKAYRPGEG
                     VKARKEAAKADLEREAAKVREAIANDPTLTKADKAKQTEAVAKALKAAIAAVDKATTA
                     EGINQELGKGITAINKAYRPGEGVEAHKEAAKANLEKVAKETKALISGDRYLSETEKA
                     VQKQAVEQALAKALGQVEAAKTVEAVKLAENLGTVAIRSAYVAGLAKDTDQATAALNE
                     AKQAAIEALKQAAAETLAKITTDAKLTEAQKAEQSENVSLALKTAIATVRSAQSIASV
                     KEAIDKGITAIRAAYVPNKAVAKSSSANHLPKSGDANSIVLVGLGVMSLLLGMVLYSK
                     KKESKD"
     gene            complement(46904..47020)
                     /locus_tag="VCI33_000373"
                     /pseudo
     CDS             complement(46904..47020)
                     /locus_tag="VCI33_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        15110 a         8160 c         9995 g        14018 t
ORIGIN      
        1 taatagctat taagtatagg acaagactaa acaagaatga cagtttggat aagagcactc
       61 ttttaaattt tgaaccgttt tcattaaaat gattgacaaa actagtttac tttgttatag
      121 tcttggtaaa caggtttact taatctaata acaaaaagga gaatcctatg gaaaatatgt
      181 tttcgttaca aggtaagatt gccttgatta ccggagcatc ttacggtatt ggttttgaaa
      241 ttgccaaagc ctatgctcaa gcgggtgcaa ctattgtgtt taatgacatc aaacaagagt
      301 tagttgataa gggggtggct gcctatcgag agttagggat agaagcgcat ggctatgttt
      361 gtgacgtgac tgatgaagct ggtatccaac agatggttag tcaaattgaa gatgaagtag
      421 gtgttattga tattctggtc aacaatgcag gtattattag gcgtacgccg atgcttgaaa
      481 tggccgcaga agattttcgt caagttattg atattgattt aaatgctcct tttattgtgt
      541 caaaagctgt attaccatca atgattgcaa agggacatgg taaaattata aatatctgtt
      601 ctatgatgag cgaattgggg cgtgaaacag ttagtgccta cgcggcggct aaaggtggct
      661 tgaaaatgtt aaccaaaaat atagcctctg agtttggaga agccaacatt caatgtaatg
      721 ggattggtcc tggctacatt gcgacgcccc aaacagctcc gttgagagag cgacaagcag
      781 atggtagtcg tcatcctttt gatcaattta tcattgctaa aacaccagca gcacgttggg
      841 ggacaacaga agacttagct ggtccagctg tttttctagc tagcgatgcc agtaattttg
      901 tcaacggcca tattctttat gtcgacggtg gcattttagc ttatattgga aaacaacctt
      961 aatcacacac tagaaagaga ttttatatga aaattgcatt aattaatgaa aatagccaag
     1021 cggctaaaaa tggtattatt tacgatgccc ttacctcagt gacagataaa cacggctacc
     1081 aagtctttaa ctatggcatg tatggcacag aaggtgaaag ccagttgacc tatgttcaaa
     1141 atggactctt agcttctatt ttattgacca ctaaggcggc cgattttgtg gtgactggtt
     1201 gtgggacagg tgtgggagct atgttggcct tgaacagctt cccaggggtt acctgtggtt
     1261 ttgcgagtga gccaacagaa gcatatctct tttctcaaat caatggtggt aatgccttgt
     1321 ccattccttt tgccaaaggt tttggttggg gagcagagtt gaatttgacc ttaatatttg
     1381 aacgcctctt tgcagaacca atgggtggcg gttaccctaa agagcgagct attcctgaac
     1441 agcggaatgc acgtatttta agtgatctga aaaaaattac ttacagagat ttgttggcaa
     1501 ttgtcaaaga catcgaccaa gatttcctca aagaaacgat ttctggcgct cattttaaag
     1561 agtacttctt tgccaatgcg gagccgtcag aattagtcgc ttatttgaaa tcagtacttg
     1621 aacagtaatt ccattttgac gaggaaagga ggtcacatgt ctaagttatt attggttggt
     1681 gaaccgctga ttcgcgttag ccccaatcag tttcagcctt tgaccaatgc ctgcgacgct
     1741 cagctttttt tcggcggttc agaagctaat attgcaagaa ctctaggcgg ttttggctta
     1801 gaagcaaggc tatttacggc cctaccagat aatcctgttg gtcacgcttt tcatcagttt
     1861 ttgaagcaaa gcggtgtgga catgtccttg acggcttggc aaggtcatcg cgttgggcta
     1921 tattaccttg aaaatggctt tggctgtcgg gctagtcagg tttattatga ccgctgtggc
     1981 tctagctttt cggccttaga taaggatagt cttgatttag ctgccatttt tgaaggcatt
     2041 agtcactttc attttagtgg gatcagtttg gcgctgggca aaaaaactca ggatctgatt
     2101 gagatactag cgagagaagc taggaaaagg caagtttgtg tttcttttga tcttaacttt
     2161 agatctagta tgattgcggt agctgatgct aaacgactct tttctcactt tgctcaatac
     2221 gcggatatta tttttggcat ggaacctttg ctgttagata gtgacgattt tgacatgttt
     2281 gataggaata aggcagatac tacgatgatt agagaacgtc tggcaggact ttatcagcgc
     2341 tatcagttac aagctattta ccatacggag cgttcaaatg atgcccaagg cagtaatcgt
     2401 ttcaaggctt atgcctatga tgggcagttt tacgaatcct gtgaagtgac gaccccagtc
     2461 ttgcaacggg ttggaagtgg agatgctttt gtggcgggtt tactttatca gttgttagaa
     2521 ggtaacgaaa agcagcgcaa ccttgatttt gctgtggcaa cagctagcct aaaatgtaca
     2581 gtagctgaag atcagctcta tcacagcgtc caacaagtag aagcagtctt acataatcaa
     2641 cgggatgtcc agaggtgatg atatgggaaa aatagaaatc ttaaccaaac ttaaggctaa
     2701 tcggcttgtc ctggttgtac gaggagagtc tagtgaggaa gctttagctt gcagcctggc
     2761 cagtattgaa ggtggcatta agacaattga agtcacctat actaacccct ttgctagtga
     2821 ggttatcggt caattagctg aacgcttcaa agaagatcct gaggtattga taggtgctgg
     2881 gacagttttg gatgatgtca ctgcccgcca agctatttta gcaggagcac agtttattgt
     2941 tggtcctaat ttcaatcgag cagtggcttt gatttgtcat ggttatagca taccttattt
     3001 accaggttgt atgacggtta atgaagtggt tacagcatta gaatcagggg ttgatatggt
     3061 caaaattttt cctggttcta ctgtagggat atcctttatt cgggcaatca aatctccttt
     3121 gccacaagta gaggttatgg ttacaggcgg agtttcttca gataatctca aagactggct
     3181 agctgctgga gtagatgttc taggcatagg aggcgagttt aatcagttag cgagccaaaa
     3241 acaatataac ctaatcacta agaaagccgc tcattatatc aaaagtttgc gacaataatc
     3301 taaaaaacgt ttttaccttg gctttagtgc tagtaaaaac gtttttttat cattcattca
     3361 attaatatgc ttctgctcat gtttttccag atagtataga tataagagca gctaatttaa
     3421 caagatgagc ctgttgtttg atatatagga tataaacttt ttatgatcaa acaaaagtaa
     3481 taccttttga attcgttagt attgatctga tctaaagtga catcacttag aatggactag
     3541 ctgtacagct tttacagcaa cttatgctaa aatagaaaaa aggagaaatg ttgatgatta
     3601 aaggaattat ttttgatatg gacggtgttt tatttgatac agaacctttt tatctgaggc
     3661 gacgagaaga tttttttaag acaaagggaa ttcccattga tcatttgaac tctaaagatt
     3721 ttattggggg caatcttcaa gaattatgga aagagttgtt aggtaaaaat agggatgatg
     3781 ctatcgttaa ggcaattaca actgactatg acgcttacaa acaagcgcat aagcctcctt
     3841 atcaaaaact gttgattaca gaagtgaact cttgtcttga acagttgaaa aaacaaggta
     3901 ttaaactggc tgtggcatca aactcgaagc gtcaggatgt tttgttggcg ttggagacaa
     3961 cgcaaataaa agattatttt gaaataatcc tagcgcgtga agatgtttct agaggcaaac
     4021 cttatccaga tatctataat aaagcagtac aaaaactagg attacaaaaa aaacaactgc
     4081 ttgtagtaga agacagcgaa aaaggcattg ctgccgccaa agcggccaat ctgacagttt
     4141 ttgccattac cgactaccga tatggcattg atcagagtca agctgatcac aagatagatc
     4201 atttaggaca actgtgtgta aaaatcggtt gttttggatc gtgaagtaac gcaactaact
     4261 gatcattgat gatacttcaa gacacttaag aatacgtcta ggactttcta cagaccaata
     4321 acaagaatac gtttatgtgt ttttactgtt ttttagctac ctatttacaa tatgacactg
     4381 aaaagagtac tatgtttaag ttattatcaa aaaaaaccgt cgcataagat cacctagaaa
     4441 aggattatga tttttgtgat ttggatgcga tcattagtcg ttgaccatct ttatgtggca
     4501 ggagatatgt ttaaccgtgg tcattatttg gatttgtttt tagattggct agtgagtaaa
     4561 tatagaagga tcataatact gctttgacaa aggcagtatc tgagtccatg agttgcacgg
     4621 gtaatgtgac tcggttttgg ttaggtataa taatatttgg aagtgttatg ctgcttgtct
     4681 tcgtaggtcc attgcttata gtcagactta ctttagagct gagctgactg tctagacaag
     4741 acgtgatgga gatgtgattt ctgaccaagt agaggtaacc agaaaaggga cttttacgat
     4801 gtaattgaat ttacttgttg cattcttgtg catgttaaga gagaaaaccc agataaatca
     4861 ggaagtaaaa tggttatgtt tgattagaat tgtccttctt tttccttgaa gtcttaactt
     4921 attttgatta tgatagtagt atgactatta aggcagaaat taaggcactc gccaaagaaa
     4981 ttggtatctc taaaattggt tttacgacgg cagataactt tgattatttg gaaaagtcat
     5041 tgcgtgcttc tgtggaagag gggcgcaatt caggatttga acataaagtc atggaggatc
     5101 gtatctaccc tgaaaggctt ttagaatcgg ctaaaacgat tatttcaatt ggcgttgctt
     5161 atcctcacaa acttcctcag caacctcaaa aaacacctta taaacgtgga aaaattacac
     5221 ctagttcgtg ggggcttgat tatcattatg tggtcggcga aaagttagac agactatcaa
     5281 aaggcattga agagctgtgt cgagattttc ctttgcaaca aaaagcaatg gtcgatactg
     5341 gagctttagt tgatactgca gttgcccaaa gagcgggtat tggttttata ggaaaaaatg
     5401 gtttagttat ttctaaagaa tatggctctt atatgttttt gggagaatta attaccaatt
     5461 tagagatcga gccagacaag cctgtcgatt atgattgtgg tgactgtcgt cgttgtctag
     5521 atgcctgtcc aacttcttgt ttaattggtg atggctcaat gaatgccaag cgttgtctat
     5581 catttcaaac ccaagataag ggaatgatgg acatagagtt tcgaaaaaag attaaaacag
     5641 ttatctatgg ttgtgatatt tgccaaattt gctgtcctta taacaagggt ataaacaatt
     5701 caccagctac agagattgat cctgagttgg ctcaaccaga attaatacca tttttgagtt
     5761 tatcaaatgg acaatttaaa gaaaaatttg gtatgattgc tggaggttgg cgaggtaaaa
     5821 atattttaca gcgtaatgcc attattgcgc tagcaaatgc tcatgataaa accgcagtgg
     5881 tcaaattaat agagattatt gataaaaata ataaccctat tcatactgca acagctatat
     5941 gggcattggg agaaattgtc aaaaaaccaa acgatgagat gctggcgttt atgagtcatt
     6001 taaccttaaa agatgaagat agccaaaaag agttggaatt aatccgtcgt aaatggcaag
     6061 tttaagcctt ttatggtatt tttaagaacc ttatgataaa ataaaacaga ttaacaaaga
     6121 ttggagaaat ctcatggaag tggctgaaat tcgccaaaaa atagtagaaa ataaagagaa
     6181 gttgactagc ttcaggaggt ctctttgact tagatcgttt ggaagaggag attgctcttc
     6241 ttgaaaatca catgactgag ccagactttt ggaatgacaa tattgcagcg caaaagacat
     6301 ctcaagaact aaatgagtta aaaggtaagt atgatacttt ccataatatg caagaattat
     6361 ctgacgaaac agaactctta cttgagatgc ttgacgaaga tgattctttg aaagaagaat
     6421 tagaagaaaa tctaatgcag ctagataaga taatgggtgc ttatgaaatg acgcttctct
     6481 tatctgaacc ctatgaccac aataatgcta tcttagaaat ccatcctggt tcaggaggaa
     6541 ctgaagcgca agattgggga gatttactct tacgaatgta tacccgtttt ggcaatgcta
     6601 atggtttcaa agtagaagta ttggattatc aagcaggaga tgaagctggt attaaatcgg
     6661 ttaccctctc ttttgaaggc cccaatgctt atggtttgtt gaaatcagaa atgggagttc
     6721 atcgcctcgt tcgtatatca ccttttgatt cagctaaacg tcgtcatact tcatttgctt
     6781 cagttgaagt gatgccagag ttagatgata cgattgaagt agaagtacgt gatgatgaca
     6841 ttaaaatgga tactttccgt tccggaggag ctggtggtca gaacgtcaat aaagtatcaa
     6901 caggtgttcg tttgacgcat attccaactg gaattgtggt atcgtctacc gttgaccgta
     6961 ctcaatatgg gaaccgcgat cgcgctatga agatgttgca ggctaagcta tatcagttag
     7021 aacaagaaaa gaaagcacaa gaagtagatg ctctcaaggg tgataaaaaa gaaatcactt
     7081 ggggaagtca gattcgttct tatgttttca ccccttatac gatggtgaag gatcatcgta
     7141 ctaactttga gcttgctcag gtagataagg ttatggatgg agaaatcaat ggctttattg
     7201 atgcctacct taagtggcgc attgaagact aaaaataata aaggagaaac atggcactaa
     7261 tagaaatgtc tggtgttact aaaaaatacc gccgctcaac gaccgccctc agagacgtca
     7321 atgtttctgt taatcaaggt gagtttgttt atctcgtagg cccatcaggt gctggtaaat
     7381 caacttttat caaactcctt taccgtgaag aacaactgac aactggtaag ctatatgtgg
     7441 gtgaattcaa tttaactaaa ctaaaagcac gtgatgttcc gattcttcgt cgtcacatcg
     7501 gagtagtttt tcaagattac aaactgctac caagaaaaac agtctttgaa aatgtagctt
     7561 acgccatgga agttattggt gaaaagcgcc gccacattaa aaaacgcgtg ccagaggtac
     7621 tcgatttagt tggcttgaaa cataagatgc gttcttttcc aagtcagcta tcaggagggg
     7681 agcagcaacg tgtggccatt gcgcgtgcga ttgttaacaa ccctaaatta ttgattgcag
     7741 atgaaccgac agggaattta gatcctgaga tttcatggga aattatgcag ttacttgaac
     7801 gtattaatgt tcagggaaca actatcttaa tggcgacaca caacagtcat attgtgaata
     7861 catttcgtca tcgtgttgtt gctattgaag atggtcgcat agtacgtgat gaagaaaaag
     7921 gagattatgg ttacgatgat tagatacttt ttccgtcata tttgggaatc cattaaaaat
     7981 cttaaacgca acttttggat gacatttgct tcagttagta tggtggctgt tacattgact
     8041 ttagttggtg tttttgcggc gacattgttg aatatacagc gtgttgcttc gggtgttgaa
     8101 aataatgtcc atatcaatac ctacttacaa gttgattcaa ctgatgctgc aaaagttatt
     8161 caaaatactg ccggtgaacc tgttaataac gataactacc acagtgttta cgacaaaatt
     8221 gctcagataa aaggtgtcaa aaagattaca ttttctagca aggatgaaca attgaaaaaa
     8281 ttgcaagaaa ccttaggtga tgtttggaat atgtatgacc aagacactaa tccgctccaa
     8341 gatatctatc ttattgagac ccagacgcct aaacaagtta aggcgatcac taaaaagatc
     8401 agaacgattg aaggcgtaga agccgcagat tatggaggga ttaactccga taaactattt
     8461 aagttttcaa cattaattca aacttggggg ttgatcggaa cagcaatgct attatttgta
     8521 gctgtctttc tcatttcaaa cactattcgt atgactatta tgtctcgtaa acgcgacatt
     8581 gaaattatgc ggttagttgg tgctaaaaat tcttatattc gagggccttt cttttttgaa
     8641 ggggcttggg ttggcttgtt aggagctgtt ttaccatctc ttctgattta ttatggatat
     8701 gatcttgttt ataagcattt tgcacaagaa ttacaacgta ataatctctc gatgtatccg
     8761 cttgatcctt atgtatatta cctgattggt gctttgtttg tcataggtat tatgattggt
     8821 tcgcttggtt cagtgctttc tatgagacgt tatttaaaat tctaaaaaaa gcgctgctga
     8881 cgaaacgtag cgaccctcaa aagttggacc tcttatgtta ttccaaggac ttcgttttgt
     8941 attgcacagg actggtcctt ttcattttgt tttgattcat ttgtttattt gtaataggca
     9001 atatagttta aaatagcagt ttctaaggtt tcaagagaag aaaagcaccc caaaagttag
     9061 atgtttgatg tatctaactt ttggggtgtc attcaaaatt tagtggctct tttttagtaa
     9121 aatactatga tttcagtttt aggataacct caaacagtat ctaaaatagc tatgtcaatg
     9181 aaaaaagggg ttagcatttt tttcgtgaca aatggtagta gaaggaccat gcccaggata
     9241 aactcggtaa tgattaggta aggtgaatag ttcttggcga ataccagtaa tgaggtcctc
     9301 aaagttactg gtaggtaagt cagtacgacc gatagtttca cgaaatagag catcgccagt
     9361 aacgactaat tcatctgagt ggaaaacaaa tgagactcct ccccaagaat gaccaggtgt
     9421 tggtaaaact gtgaattcaa aaccatttag ttgatagggt tgctttaatt taaaaaagtt
     9481 ctctgccggt cttgctataa cagtaatgat atcatcatga cggcctaacc cagataaatt
     9541 atcatctggg gaagatagcc aagcggcttc tttttctgaa acataaacag gtggatgatc
     9601 aaaagcatct ctgaccaaat ctaaactaaa aatatgatca taatgagtat gcgttaagag
     9661 gatagccact aaaggttttt caaaggattt aattttagca atgatttctt ggccgttact
     9721 tcctggatca atgaggataa cagcttgatc attaacgagg taataagtat tttcaccagc
     9781 aatgtggttt attgttttat agattttcat aaggatagtg taccaaaaaa tcaaggcttt
     9841 gttaaaaaaa gagtggcgga aaataaatgg caattagttt cagaaaatat gagatctcac
     9901 agcaatttct tgacaccgtt ttcataaagg tgtatcataa aagtaatcaa taagccaagc
     9961 ctaaagctga acttagttta agaacactga ctttttgaga gcaacctaat cggttgacta
    10021 aaaaatatga ggtggaagat atgtcaaaag ttgctattgt tactggtgct ggtcaaggaa
    10081 ttggatttgc tattgcgaaa cgtcttcatg cagacggctt taaaataggc attttagact
    10141 ataatgaaga gactgctcag gctgcggttg ataaactatc acctgaggat actgttgcag
    10201 ttgtagcaga tgtttcaaaa cgtgaccaag tttttgatgc tttccaaaaa gttgtagata
    10261 cttttggtga tttgaatgtt gttgttaaca atgcaggtgt tgccccaaca acccctcttg
    10321 acacgattac tgaagagcag tttgaaaagg cttttgcaat caatgtcgga ggaacgattt
    10381 ggggaagcca agcagctcaa aaacatttta gagaattagg tcatggcgga aaaattataa
    10441 atgcaacgtc acaagcagga tgtgaaggga atcctaattt gactgtttac ggtggtacaa
    10501 aatttgcagt tcgtggtatt acacagactt tggcaaagga tttggcatca gaaggaatca
    10561 cagtcaatgc ttacgcacca ggtattgtta aaacacctat gatgtttgat attgcccatg
    10621 aagttggtaa aaatgcaggc aaagatgatg aatggggtat ggagcaattt gcaaaagata
    10681 tcactttgaa acgcttgtct gaaccagaag atgttgctaa tgcagtaggc ttcttagtgg
    10741 gagatgattc aaattatatc actggacaaa caatagttgt cgatggtggt atggtattcc
    10801 attaaaaatg attaatactg acactgtgag tttcactcac agtgtttttt atatttctta
    10861 aaaaattatc ctatcttgat tattggagtg atggcacctt tgaggtatag tcatgttttg
    10921 ttttatagat atagcgtgct ataatagact tactatgacc cagaaaaaac ttagaaaata
    10981 tgcagtagtg gacttggagg ctactggagc aggtcccaat gcttctatta ttcaggttgg
    11041 gattgttatt atccaaggaa ataaaattat tgatagttat gaaacagatg ttaatcctca
    11101 tgaatctttg gatgagcata ttgttcattt gacaggtatt acggataagc agttggctaa
    11161 agctccggat tttggtcaag tggcgcatca catttatcaa ttgattgaag attgtatttt
    11221 tgttgcccac aatgtaaagt tcgatgcaaa tttattggca gaagctcttt ttttggaagg
    11281 atatgagtta acaataccac gtgttgatac tgttgaatta gcccaactat ttttccctcg
    11341 ctttgaaaag tataacctta gtcacctatc taggcagctc aatattgatt tggcagaagc
    11401 tcatactgct attgcagatg caagagctac agctatactg tttttaagac ttttacaaaa
    11461 aattgagagc cttccaattg agtgcttaga aagcttatta gcctattcag atagcctgct
    11521 atttgaaaca gcaatggtca ttcaagaagg attagccaag gccaaacctt atgatcccaa
    11581 caaatacatt aaaatcagac agattctctt accaaagggc tcaaaagctc tcaagcctta
    11641 tcaaatatca aaaagcttcc ctattaatat ggcactatta ggtctggaag aaaggcctaa
    11701 acagactcaa tttgctcagt taattgacga agattaccat cagggagtag ctagttttat
    11761 tgaagctcag acaggtattg gaaaaaccta cggctacctg ctaccccttt tggcaaaaga
    11821 agatcaaaac caaatcattg ttagtgtgcc aaccaaactt cttcaagacc aattaatggc
    11881 tggtgaagta gctgctattc aagaacaatt tcacatcgcc tgccatagtt taaaagggcc
    11941 tgccaattac ctaaagctag atagctttgc agatagtttg gaccaaaatg accagaaccg
    12001 tttggtgaat cgttataaaa tgcaattatt ggtttggctc ttagaaacta agacaggaga
    12061 ccttgacgaa atcaaacaaa agcagcgctt tgccgcttat tttgagcagc ttaagcacga
    12121 tggagacatt aagcagtctt ctgaatttta cgattatgat ttttggagag ttagttatga
    12181 aaaagccaaa acagcccgtc tcctgattac caaccatgct tattttttac accgtgttca
    12241 agatgacaag gactttacta gaaacaaggt cttggtattt gatgaggccc aaaagctgat
    12301 gctccaatta gatcaattat cacgtcatca gcttaacctg acggtttttt tacaaacaat
    12361 acaggccaag ttaagcaacc ctttacctct tttagaaaaa cgcttattgg aaagcttgtc
    12421 gtttgaactg ggacaagtgt caagtgatta ctatcaaaat aaagagcacc agcttgctca
    12481 tgattggtct cgtatagctg gctacgccaa agaattaaca ggagcagatt atcaagagtt
    12541 acaagccttt tttgctacct cagatggtga ctactggctt agtagcgaaa agcaagagga
    12601 gaagcgagtg acttatctaa acagtgctag taaggcattt attcatttcc aacagctctt
    12661 accagaaacg gtgaaaactt attttgtttc tgccacttta accatcagct cagaagtaac
    12721 cttggcagac ctattaggtt ttgaagagta tctctatcat gtcattgaaa aggataaaaa
    12781 gcaagaccag ttggtgcttg ttgatcaaga ggcgcctatc gtgacagaag tcagtgacca
    12841 aatctatgtg gaagcaattg caaaacgaat cgaaagcttg aagcaagagg gttatcctat
    12901 tttagtgctt tttaattcta aaaaacactt gctgttggtt tcagactatc tagaccaatg
    12961 gcaagtgcct cacttagcgc aggaaaagaa tggaacagct tataatatta aaaaacggtt
    13021 tgatcaagga gagcaaacta ttttgcttgg tttaggttct ttttgggaag gagttgattt
    13081 tattcaagcg gatcgtatga ttacccttat cgcacgattg ccctttgata acccagagga
    13141 tttctttgtt aagaaaatga gtcattatct attggaaaaa ggtaaaaatc ctttcagaga
    13201 ttacttcttg ccaatgacta ttttgcgatt aaaacaggct attggtcgca cgatgcgtcg
    13261 acaagatcaa aagtcagtgg ttattatttt ggatcgtcgt ctccttacaa agtcctatgg
    13321 acaagttatt ttggaaggtc tcggtcagga atttttgatt tctcaacaaa attttcacga
    13381 ttgtctagta gaaacggact gttttctgat ataatggaaa ggatctatta tatcagaaaa
    13441 atttttaatg gcgttaagca caatcaacta acatttaaat tagtttgaac ttttattatg
    13501 ctcttttaga caactgttca aatgttttcg aattgttttt ttgataagga caataaatga
    13561 acaaattttt ggttttagta aacccaatta ctgttctttt cattaatgtc agttgctatc
    13621 aaaacagctt gtcatagttc gtgcgatcag tagcgagtat tggtaaaact gaaaccgtag
    13681 attatgaaaa aatgatctgt tgatcattaa ttaatctgaa gataagaagt cgataaaaag
    13741 gagatacgat gcccaaacta tcaaagcgag ttttagagat gaaagagagt gttactctgg
    13801 ctgcaggtgc tagagctaag gctttgaaag cgcagggtcg tgatgtactg aatttaactt
    13861 taggagaacc agattttttt acacctaagc acattcaaga caaggcgatt gaatctattc
    13921 aaaatggtac ggctagcttt tatacaaatg cttctgggct tccagaatta aaagctgcga
    13981 ttgccacata cctgaaaaat cagtatgggt atcacttgtc tcctgatcag attgtggcag
    14041 gaacaggtgc caagttcatc ctttatgcct tttttatggc tgttttaaat cctggtgacc
    14101 aagttctcat tccaacacca tattgggtat cttattctga ccaagtaaaa atggcagaag
    14161 gtcagccgat cttcgttcaa ggactagaag aaaatcaatt caaggtaact gttgaccagc
    14221 tggaaagagc aaggacaagt aagactaaag ttgtccttat caattctcct tccaatccaa
    14281 caggcatgat ttatggtgct gaggaattaa gggccattgg agaatgggcg gtgcacaacg
    14341 atattcttat tctggcagat gatatttacg ggtccttagt ctataacggc aatcagtttg
    14401 ttcctatttc aacactctca gaagcgattc gtcgtcaaac cattacggtc aatggcgtgg
    14461 ctaaaagtta tgccatgaca ggctggcggg ttggttttgc agcaggtgaa ccagagatca
    14521 tttcggcaat gagtaagatc atcgggcaga ctacctctaa cctgacaacc gtttcacaat
    14581 atgctgctat cgaggctttc tgtggtagtc agtcatcact tgaagagatg cgattagcat
    14641 ttgaagagcg ccttaatatc acttatccat tgttgtgtca ggtccctggg tttgaggttg
    14701 ttaaaccaca aggagctttt tacttctttc caaacgttaa gaaagccatg gaaatgacag
    14761 gtttttccga tgtgacaagc tttgctaatg ctattttaga ggaagtcgga ttagctgttg
    14821 tgagtggagc aggctttggc gcaccggaaa atgttcgtct gagttatgct acagatatcg
    14881 aaacccttaa agaagctgtt cgtagactgc atgtctttat ggaaagtaat gaaattaatt
    14941 aaattaatta aataaaaata gagagaaact tatgtcaaaa aaacttattt caattgttga
    15001 tgttaaagat tatgttggtc aagaagttac tattggtgct tgggttgcta acaaatcagg
    15061 taaaggtaag attgcgtttg tacagttgcg cgatggttcg gcttttttcc aaggtgtcgc
    15121 ttttaaacca aacttcattg aaaaatatgg tgaagaatca ggtcttgaaa aatttgacgt
    15181 tattaaacgt ctcaaccaag aaacatctgt ttatgtaaca gggattgtta aagaggatga
    15241 gcgttctaaa tttggctatg agcttgatat tactgacctt gaaatcattg gagagtcaca
    15301 tgaatatcct ataactccta aagagcatgg gacagacttc ttaatggaca accgtcatct
    15361 ttggttgcgt tctcgtaaac aaatggctgt tatgcaaatt cgtaacgcta ttatttatgc
    15421 aacttatgaa ttctttgacc aaaatggttt tatcaaattt gatagcccaa ttttatcaga
    15481 aaatgcagca gaagattcaa cagagttatt cgaaacagac tattttggga aaccagcatt
    15541 tcttagccaa tctggtcaat tataccttga agctggagca atggctctag gccgagtttt
    15601 cgactttggt ccagtctttc gtgctgaaaa atctaaaaca cgtcgccatt taacagaatt
    15661 ttggatgatg gatgctgagt attctttctt atcacatgag gaatcacttg accttcaaga
    15721 agcttatgtt aaagcattga tccaaggcgt tctagatcgt gcgcctcaag cacttgatat
    15781 tttagaacgt gatgttgagg ctctcaaacg ttatatcaca gaaccattca agcgtgtttc
    15841 ttatgatgat gctattacac ttcttcaaga gcatgaagca gatgaagata cagattatga
    15901 acatttagag catggtgatg actttggttc cccacatgaa acttggattt ctaattactt
    15961 tggagtaccg acatttgtag tgaattaccc agcaagcttt aaagcatttt atatgaaacc
    16021 agttcctgga aatccagaac gcgttctttg tgctgatctt ttagctccag aaggttatgg
    16081 agagatcatt ggcggctcaa tgcgtgaaga caactatgat gctcttgtgg ctaaaatgga
    16141 tgaactaggt atggataaat cagaatatga cttttacctt gaccttcgta aatatggctc
    16201 tgttcctcat ggcggttttg gtataggtat tgaacgtatg gtaacttttg ttgctggtac
    16261 caaacatatt cgcgaagcta ttccattccc acgtatgtta catcggatta gaccctaata
    16321 atacaaaaaa gttcatgtca tctatgaact ttttgtattg gtaagaggtg agtgtgttat
    16381 aaaaaaatca atatgtccta aaggagagat aagaaatagt agagtttgaa cctacatcct
    16441 ttgtgaagat aaattttgga agtttagtag atcagttagc agataagaca atatagcctt
    16501 gctcaaggga tattttatag acaagtattt ggactacgat tatcaaatag acagtctcat
    16561 ttgatactaa aaaggtctcg tctattcata taataggaga gttacgggag ttattattaa
    16621 aattagctga tttttacagt tcaaaatgtg ttaccacttt ttcacttctt agctttctta
    16681 tgtatgtggt ataatgaagg tggacggttc gatagagaaa gggaaattgt aaatgtcaga
    16741 caaacacatt aatttagtta ttgtgacagg aatgagcggc gctggaaaaa cagttgccat
    16801 tcagtctttt gaggatctag gctactttac cattgataat atgcccccag ccttggttcc
    16861 aaaattttta gaattaattg aacaaaccaa tgaaaatcgt agggtggctt tggttgtcga
    16921 tatgagaagt cgtttgtttt tcaaggaaat taattctacc ttagatagta ttgaaagcaa
    16981 tcctagcatt gattttcgga ttcttttttt ggatgcaacg gatggagaat tggtgtcacg
    17041 ctataaagaa accagacgga gccacccttt ggctgcggac ggtcgtgtgc ttgatggtat
    17101 tcgattggaa agagaactcc tatctccttt gaaaagcatg agccaacatg tggtggatac
    17161 aacaaaattg acccctagac aattgcgtaa aaccatttca gaccagtttt ctgaagggtc
    17221 taatcaagcc tctttccgta ttgaagtgat gagctttggg ttcaaatatg gtcttccttt
    17281 ggatgcggat ttggtttttg atgtgcgttt tctacccaat ccttattata aggtagagct
    17341 tcgtgaaaaa acaggactag atgaggacgt ttttaattat gtgatgtctc acccagaatc
    17401 agaggtgttt tacaagcatt tgttaaacct tattgtccct atcttaccgg cttaccaaaa
    17461 agaagggaag tctgtcttga cggtggctat tggctgcaca ggaggccaac accgcagcgt
    17521 tgcctttgcc cattgcttgg cagaaagtct ggcaacagat tggtcggtta atgaaagcca
    17581 tcgtgatcaa aatcgtcgta aggaaacggt gaatcgttca tgaaaaatcc taaaatgact
    17641 gttataggtg ggggtactgg catctccatt atcctaaaaa gccttcgtaa tgaagcagtt
    17701 gatattactg ctgtggtcac tgttgcagat gatggtggtt cctcaggaga acttcgtaat
    17761 gccatgcaat taaccccgcc aggagactta cggaatgtct tgttagcgat gagtgacatg
    17821 ccaaaatttt atgagcgggt tttccaatac cgttttaacg agagtgatgg agccttggct
    17881 ggtcaccctc taggcaatct cattattgct ggtatctctg aaatgcaagg ctcaacctac
    17941 aatgccattc aaattttaac caaatttttc catattacag gtaaaattta cccctctagc
    18001 gagcaggcct tgacccttca tgctgttttt aaagatgggc acgaagtagc aggagaaagt
    18061 tctattgcca aatatcaagg catgattgac catgtctatg tgaccaacac ctacaacgac
    18121 caaaagcctc aggccagtcg taaggtggtt gaggctattc tagaaagtga catgattgtc
    18181 ttgggaccag gctcactgtt tacctctatt ttacctaatt tggtcattcc agaaattaaa
    18241 gaagctttgc gccagactaa agcagaagtg gtgtatattt gtaatatcat gacccagtat
    18301 ggtgaaacag aacaattttc agatgcagac catgtggctg ttttaaatca gcacttagga
    18361 agagatttga ttgatacggt tttggtaaat gtggctaagg tgccgcaggc ttacatgaat
    18421 tctaacaaat ttgatgaata cctcgttcag gttgatcacg actttgctgg cctttgcagg
    18481 gctgctaagc gtgtgatttc gtcttatttt ttacggttgg agaatggcgg agcttttcac
    18541 gatggtaact tagtggtgga agaattaatg aatttagtga ggatagtcaa gcaatgagtt
    18601 ttacaaccaa ggtcaaagag gaattaattc acttgtccac aggtgacaac aatgaattgg
    18661 ccgccattat caaattatca ggcagcttgg gcttagctca ccaaagcctt catttatcca
    18721 ttaccactga aaatgctaaa attgcccgct acatttattc tcttattgaa gacgcctatg
    18781 ttatcgtgcc agaaatcagg tatcatcaaa agacgaacct gcgcaaaaac cgtgtttaca
    18841 ccgtctatgt tgagcaaggt gtggaaacca ttttagccga tttgaaatta gcggactcct
    18901 tttttggtct ggaaacaggc atcgaacctc aggttttatc agacgataat gctggccgtt
    18961 cttatcttaa aggggctttt ctagcggcag gtagcatcag agatcctgag tcaggcaagt
    19021 atcagttaga aatctattca gtttatcttg atcatgctca agatttagct caactcatgc
    19081 aaaaatttat gctagatgcc aaaaccatcg agcataaaag cggtgcagtt acttatgtgc
    19141 aaaaggcaga agatatcatg gactttttaa ttataattgg tgccatgtcc tgcaaagaag
    19201 actttgaagc catcaaatta cttcgagaag ctcgaaatga tattaaccgt gccaataatg
    19261 cagaaacagc taatattgcc aaaactatca gtgctagcat gaaaaccatt aataatatta
    19321 ttaaaatcat ggacacaatt ggtttagaaa gtttaccgat tgaattgcaa caggtagccc
    19381 agcttcgggt taagcatcca gactattcca ttcaacaagt tgctgatgct cttgagttcc
    19441 caatcaccaa gagcggggtc aaccatcgct tgcgtaaaat caataaaatt gcagatgact
    19501 tatagaacat ttttatcgta tgagcctttg ttcatacgat ttttttgtct aaaagtcgtt
    19561 ttccattgtt tttttgaagt gtctgatgtt ctgtgatatg ataaaaggga taataacgtt
    19621 tgtaaaggag actgataatg gcatgtacaa ctatactcgt cggtaaaaag gcatcttatg
    19681 atggctcaac catggttgct cgaacagaag attctcaaaa tggtgatttc acgcctaaaa
    19741 aaatgattgt ggtgaaacca gaagatcaac cgagacatta ccgttcggtg caatcttcct
    19801 ttgaaatgga cttaccggac aatcccatga cttacacgtc tgttcccgat gcgcttggta
    19861 aagatggtat ctgggcagaa gcaggcgtga acgaggctaa tgtggccatg tcagcaactg
    19921 aaaccatcac taccaattct cgtgttttag gggcagatcc tctagtagct tctggtattg
    19981 gtgaggagga catggtgacc ttagtgctcc cttatatccg ttctgcgcgt gaaggggttt
    20041 tgcgccttgg tgccatttta gaagactatg gcacttacga gtcaaatggt gtggcttttt
    20101 cagatgaaca tgacatttgg tggctggaaa cgattggtgg tcatcactgg attgcaagac
    20161 gggtgccaga tgatgcttat gtcaccaatc ccaatcaatt tggcattgat tattttgaat
    20221 ttaacaatcc tgaagattac ctttgctcag ctgatttaaa ggactttatt gacacgtatc
    20281 acttagattt aacctataac catgagcatt ttaatccccg ttatgccttt ggtagtcaac
    20341 gtgacaaaga ccgtcactat aatacgccac gtgcttggat catgcaaaaa ttcttaaacc
    20401 cagaaattgt gcaagacccg cgcagctttg cccttgcttg gtgtcaaaaa ccttaccgta
    20461 aaataacagt agaagatgtc aaatatgtat tgagtagtca ctatcaggac acggtttatg
    20521 acccttacgg ttcagaagga actcctgtta gtaagaaagt ctttcgccct attggcatta
    20581 accgtaccag ccaaaccgct atcttacaca ttcgtcctaa taaacctcaa gaaattgcgg
    20641 ctatccagtg gatggcttat gggtcaatgc catttaatac catggtgcct tttttcacac
    20701 aggtgaagac tattccagat tattttgcca acacttacga aaatgtgtct accgacaatt
    20761 tctattggac caaccgtctg attgcggctt tggcagatcc tcactacaat catcatgaaa
    20821 ctgatttgga caattacttg gaagaaacca tggcaaaagg tcatgccatg ttacatgctg
    20881 tggaagcaca gttattggct ggtgaaacag tcgatttgga agaggaaaat caaaaaatga
    20941 gtgactatgt gcaaggagaa acgcagacgc tgttgaacaa gattctcttt gacgctagca
    21001 atctaatgac caatcgtttc agtttgagcg attaaaagtg aagctaaggc tgagcaactg
    21061 cttagccttt tttacgataa ctttttagga aaagtaatcg caaaaagaca aaaacttgga
    21121 aaaaaaatca aagtatgcta tacttaactg gtcaattttt aaccagttaa taacaaagga
    21181 ggaatcatga aaaagaaaat tcttttaatg atgagtttaa tcagtgtctt ttttgcttgg
    21241 caacttactc aggcaaaaca agtcttagca gagggtaaag tgaaggtggt gacaactttc
    21301 tatcctgttt atgaatttac aaaaggggtt attggtaatg atggcgatgt ttccatgctt
    21361 atgaaagcag gaacggaacc tcatgatttt gagccttcta caaaagacat taaaaaaatc
    21421 caagatgcag atgtatttgt ttatatggat gacaatatgg aaacttgggt ttctgatgtg
    21481 aaaaaatcat tgacatctaa aaaagtgacc atcgtcaagg gaactggtaa catgctcttg
    21541 gtagcaggag ttggacatga ccatcaccat gaggaagctg acaaaaagca tgagcataat
    21601 aaacatagcg aagaaggaca caaccatgct tttgacccac acgtgtggtt gtcaccatac
    21661 cgtagcatta cggtcgttga aaatattcgc gacagtcttt caaaagctta cccagaaaaa
    21721 gcagagaact tcaaagccaa tgccgctact tatattgaaa aattaaaaga gcttgacaaa
    21781 gactatacgg cagcactttc agatgctaag caaaagagct ttgtgacaca acacgcagct
    21841 tttggttata tggcacttga ctatggcttg aaccaaattt ctattaatgg tgtcacacca
    21901 gatgcagaac catcagcaaa acgtattgct actttgtcaa aatacgttaa aaaatatggt
    21961 atcaaataca tttattttga ggaaaatgcg tcaagtaaag tcgcaaaaac cctagctaaa
    22021 gaagcaggag ttaaagcggc tgtgcttagt ccgcttgaag gtttgactga aaaagagatg
    22081 aaagctggcc aagattactt tacggtcatg cgtaaaaacc ttgaaacctt acgcttaacc
    22141 actgatgtgg ctggtaaaga aattcttcca gaaaaagaca cgactaagac agtttacaat
    22201 ggttatttca aagacaaaga agtcaaagat cgtcaattat ctgactggtc aggtagctgg
    22261 caatctgttt acccctatct acaagatggt actttagacc aagtttggga ctataaggct
    22321 aaaaaatcta aaggtaaaat gacagcagcc gagtacaaag attactacac tactggttat
    22381 aaaactgacg tggaacaaat caaaatcaat ggtaagaaaa agaccatgac ctttgttcgt
    22441 aatggtgaaa agaaaacctt cacttacaca tacgccggca aagaaatctt gacctatcca
    22501 aaaggaaatc gcggagttcg tttcatgttt gaagctaaag aaccaaatgc tggcgaattc
    22561 aaatacgttc aattcagtga ccatgccatt gctcctgaaa aagcagagca tttccacctg
    22621 tactggggtg gtgacagcca agaaaaatta cataaagagt tagaacattg gccaacttac
    22681 tacggttcag acttatctgg tcgtgaaatt gcccaagaaa tcaatgctca ttaatcttaa
    22741 ctcactaagc aaatcttctt ttaaggaggt ttgctttttc tttgtggagt aaaaaaattt
    22801 aataattctc aacattaatt tgacggacac ttatgattat aatatacttt aagtggttat
    22861 aacaagttag aggtgaataa gatgccaaaa gaacaaccac tctatctaca gattgttgat
    22921 gacttggaag tgaaaatcaa aaagagcatg acagaaaacg ataagttgtt gtcagaacga
    22981 gaattaagtg acctttacgg tgttagtcgt atcacgatta gactggctct taaggaattg
    23041 gaattacgag acttaattta taaaaagcag ggcaagggga cttatgtttc aggaatcaaa
    23101 gaacctgtca ctgatttatc aagtacttac agctttacag agcaaatgaa aaaagtgggg
    23161 aaaactccta aaacagagat tctttctttt gaacagtatc aggtaacacc ttacttgtct
    23221 ggtctcttag agtttgatct cgatacagaa gtttttgaat tagaacgcct aaggattgca
    23281 gatgatatgc ccttgatgtt tgaacgatcc tatattcctg cgcagccttt tcaggggtta
    23341 agcattgcgg acttgaagcg taaagctttg tacgatatct ttgcaaagga ctatcaagag
    23401 acaatccgtc ttgcagaaga agagttttat gccagcattg ccttggacta tgaagcagac
    23461 cttttgggaa tcaaaaaagg agatcctgtc ttacatatta ttcgtaaaac ttataacgat
    23521 aaaaatattt tgatcgaatt tactttcagt attgcaagag cagaccagtt taggtatagg
    23581 gtacaacatc accccaatgg ttaaagaaat gggaggttaa tacatgttta caaaaacgca
    23641 ggaagctttg gaggctcttg gtgcagctat cactaccaag gaaattaaac aagaaccccg
    23701 tttatggcaa gaaaccatga cgttttttga agaaacaaga gattctctgg attctttttt
    23761 aaagagagtt tgtaaaagcg ctaatggaaa taacgttcat gtcatcttta caggtgcggg
    23821 tacatctgag tacatcggta ataccatttg cccttacttg aagaaggtgg ggaatcggca
    23881 gtgctatctc tttgagagtg ttgcttcaac cgatttggtt gcggcgccag attattatct
    23941 tgctgaggaa gagacggtac tattagtctc ctttgctagg agtggtaata gtccagaaag
    24001 tgtagcggct gttaacctgg tcaatcaact agtgccaaat agttatcatt taaccattac
    24061 gtgtgccaaa gatggagaac tggcgaaaaa agcacagcaa gatgagcgtt cttaccttta
    24121 tttgatgcct gaagacgcta atgatgctgg ttttgccatg actggtagtt ttacctgtat
    24181 gatgctagcg gctcttttga tttttgatga tgctaacagt atagctcaga aagaagctta
    24241 tgtgactgat atgattcttg ctgggcaagc tgttattgac caagaaagac ggctacaaga
    24301 gctagctgat ttaggatttg aacgtttagt ttatctaggt tctgctggtt tagccaagtt
    24361 aacccaagaa gctcaattaa aaatgttaga attgacagct ggacaagttg cgaccctcta
    24421 cgagtcctct atgggcttta ggcatggtcc gaaatctttt attaatgacc gtaccttagt
    24481 cattggattt gtcaataatg atgcttacgt cagacagtac gatttggaca tgttagagga
    24541 aatccaagca gatggcattg cccttaaaat cttggctcta ttgcaaggag gggatatcaa
    24601 cttttcagag gaccaatttc gactagacac ccgtcactta ttgccagaag cttacttagc
    24661 ttttcccatg attttagtcg cgcagacctt ggccctctta acggccgtta cattaggaaa
    24721 ttcccctgac accccatctg cgacaggaac ggttaatcgt gtggtcaaag gagtaacgat
    24781 tcacccttac ccgactagta gtcgtcaatg caacaaagct taaagctttt gtgtagaagc
    24841 cgtaaaacga aatgattttg tgctagtagg ccaaaagaaa aaccctcctg atacaggaag
    24901 gttttttgta ttgtcttgct tattttgctg cgtttgcgtc tttgagaccg tattttttgt
    24961 tgaagcggtc cacacgtcca tctgcttgag tgaacttttg acgtcctgtg tagaatgggt
    25021 gtgaatctga tgaaatttcc acacggataa gtgggtaagt ttcaccttca aactcaacag
    25081 tttctttaga agcttttgtt gagccgctaa ggaactggta acctgtagtt gtatcaagga
    25141 aaacaactgg acgataatct ggatgaatgt cttttctcat cttaaaaaat tcctttctgc
    25201 catgacactt gatgcatcat agttattaac tattctagtg taacaaaact tgaatgactt
    25261 gacaaggctt ttctttcctt attttttaaa aatcctaaat ctcttggcaa acagcgatga
    25321 gttctcggaa aatagcttga ttttcttcta aattagctga gttggctccg ctagcaaggg
    25381 gatgcccccc tccaccgtga cgcttagcaa tgccattaat aatcttttct ttactgcgca
    25441 tacgcacacg gtagttgccg tcagttaact caacaaaaat agcccaagct tgaacgttat
    25501 caattttacc aggagcacag acaatggcag agctttctgc tagggtcacg tcaaaatgtt
    25561 ttaaggtttc ttggctgaca aggacataag cagccccact ctcatcaatt gttaaatgct
    25621 caaagacgta gctttgcagc tttgctattt tcaaaggaaa cgaatccatt tgccttgaaa
    25681 tcgcagcaaa gtcgaactcg aaatgtctga gttggctagc aatggaaagg gttttactag
    25741 tggttgaggc ataaagaaat cgccctgtat caccaacgat accagtgtat aagagcttag
    25801 cagccttgtc agagagagta agattctgac taaaggcaaa gtctgcaatg atttcacttg
    25861 cgctagaagc gcttgtgtcc acataataga agtcaccata tacatcatcg ttgggatggt
    25921 gatcaatctt aattaagcac tgccccagtg tgtagcgctc atcatcaatc cttggtcgat
    25981 tcgctgtatc tgtaatgatg accaaagcct ctttatagtc tttatcagtc acctgatcca
    26041 tttggctaat ccaagttaaa ctaggctcat caaaaccagt catcaaaacc tttttatctg
    26101 ggaaattttg tgcaataatt tctttcaagc cggcctgact accaagagca tcagggtcag
    26161 gattttgatg gcgatggata ataatagttt ggtgagcttt tattttatct aaaattgttt
    26221 caaaagttgt tatcatgctt taatctttct ttttcaaaaa gaggaaaata accgtttaac
    26281 ataatcttca aaaaacggtt tgtgtcataa ggaggtgtgc gttatgttaa gtggctaatg
    26341 ctagaatttt ttaagattgt caaagctcat aaattgacat tttatctgaa aatcatatcc
    26401 tattttatta tagcataaaa gtaagctcta gataatggta aattaatcta ggaagaaaag
    26461 aggttagtta tcagttatga tactatcaaa gctagttttg caaatgcaag tgaaaaaata
    26521 gtttttaagt gtcttaagta atcatatcaa ctttctcttt aaacaattag tgcttttcct
    26581 aaaaaagtgt gctaaaataa acatagttta gtaattttgg gaggaattta tggcattagc
    26641 aaaaatcgtt tatgccagca tgacgggaaa tactgaagaa atcgctgata tcgttgcaaa
    26701 taaattgcaa gaattgggcc atgatgttga cattgatgag tgcacaacag ttgacgcttc
    26761 agagtttgag aatgctgata tcgctgtcgt tgcgacttat acttatggtg acggtgactt
    26821 accagatgaa atcgttgatt tttacgaaga tttacaagat ttagatttag aaggtaaaat
    26881 ctatggcgtt gtcggctctg gtgacacttt ttatgactat ttttgcaaat ctgttgatga
    26941 cttttcagat caatttgcgc ttactggtgc cgtcaaaggt gcagagccag tcaaagtaga
    27001 tttggcagca gaagatgaag acatcgatcg tttagaggcc tttgcagagc aactctcaca
    27061 ggctgttaat aatctataaa actatttagt aaaaatgtca gggacttcct tggcattttt
    27121 agatttttaa cctaaaataa agggaatgtc acttcagggt aggaattgaa aaaacaatca
    27181 gcttcacctt gcttctgatc ctcttattaa ctataataaa aagataatct aatacatcta
    27241 gaaaaggaaa gttatgcgtt tagaagagat acgacaagaa atcaatggta ttgatcacca
    27301 cttggttgcc ttattagaaa agcggatggc acttgtagag caagtaacag cttataaact
    27361 agccaatcac ttgcctgtct tagatcaggc gagggaaaat cagatcctag atagggtttc
    27421 ttatctcgtt aaagaccaag catttgagcc agctatccat gagactttta aaaccattat
    27481 gtctctttca cggcagtatc aaacgcaacg cttaacaggt ggtgacatta atgattaaat
    27541 caattgcaac actgcctaaa ccctccttaa aagaagcgtt aggactaata ggcttggcgc
    27601 ttcccatagg ggccacggtt ggagtggttg atgtgatttt tggtaaaggg cttttgtttc
    27661 tttccgaata tcgcgatcac cacctgttct tgcttcttcc ttttcttgcc ctagcaggtc
    27721 ttgttatcgt ttttctttac gataagttag gcaaagaggt tcgtcaaggg atgggattgg
    27781 tttttcaggt aggacatggt caaaaagatc agattcctcc tattctcatt cctcttattt
    27841 tgttctcgac ttgggtaacc cacttatttg gagcgagtgc gggtcgagaa ggggtagccg
    27901 ttcaaatagg agctaccatc tctcattatt gtcagagatt tgtgactagt caagaagccg
    27961 cacgacacct tttgattatg gggatggcag ccggttttgc aggacttttt caaacgccga
    28021 ttgctgctgt cgtatttgcc cttgaggttt tgttggtagg aacgcttcgc tactgcgctt
    28081 tattgccttc cttagttgct gcttgcgtgg cttcttggac ctctcattct ttgggattag
    28141 agaaatttac gattgttctt gaagaaacat tgaccataac tcccttaaca ttggttaaat
    28201 taattgggtt aggccttatt tttggtctgg tgggaaatag ttttgcctat ttacttggct
    28261 ggtttaaacc ttacctcagt caaaagctac caaatcctta tttccggatt gcttttatcg
    28321 gtgctttgtt atctatctgt ctgatgattg gtcacgtcgg aagatatagt ggtctaggaa
    28381 ctcatttgat tgcggcagct ttttctggcc aaactatcct aacttacgac tggcttttaa
    28441 aaatgatcgt gacggtgatt agcttatctg caggctttca aggtggtgaa gtgacgcctc
    28501 tatttgctat tggagcttct ttaggcatcg ttttggcccc ttacttaggt cttcctgtac
    28561 tgttggttgc tgcacttggt tacaccacgg tatttggaag tgcaacaaat accttttggg
    28621 cccctatctt tattgggata gaggtctttg gtccagagaa tgccctagca tattttgtca
    28681 cctcggctgc agcctatatg gttagtcaca gacattccat ttacagttat caaaaagtag
    28741 tcatgatcaa tttaaaagac atgacggtaa atcagatgga tttaaaaaaa tagctttaca
    28801 ataaagaagc tttttgttag aataaatctc gtgtgtaatg gacacacgaa aattacggct
    28861 aatccgctaa gacaagtact taagattagt aagataggag aataaaaatg aatccattaa
    28921 tccaaagttt gacagaaggt caacttcgct ctgatatccc taacttccgt cctggtgaca
    28981 ctgttcgcgt tcacgcaaaa gttgtcgaag gaactcgcga acgtatccag atctttgaag
    29041 gtgttgttat ttcacgtaaa ggtcaaggga tctcagaaat gtacactgtt cgtaaaatct
    29101 caggtggtat cggggtagag cgtacattcc caatccacac accacgtgtt gacaaaatcg
    29161 aagttatccg ccacggtaaa gtccgtcgtg cgaaacttta ctacttgcgt gcattacaag
    29221 gtaaagctgc tcgtatcaaa gaaattcgtc gttagtttga accgaaacgt aacgtaatag
    29281 aataaaaaga ctagagtctc tagtcttttt atgtcgtcgt gacaatgtga caattggtga
    29341 ttaacgctat gctcgtagga cactttgaaa gttccttagc agttataatt agtatattgt
    29401 aataacaaaa tgaagcaaaa taaactttca gttatctgat atttcatctt gcattacctt
    29461 attttctagg ttaaaataga agacaaggtg aatataagat aggcccctta gttcaatgga
    29521 tataacaact ccctcctaag gagtagttgc tggttcgatt ccggcagggg tcattattgt
    29581 cactgaaaaa gcctttacat caaaggcttt ttgttttatt ccggttttaa aaggggcata
    29641 aaaggggcag tttgtttatt tataatttct ttcatgttta cagttgtgtg actgtaaata
    29701 gataatgttg tttttggatc gctgtgacca actctatcca ttatcgcatt tagcggtatc
    29761 cctttttctg ctaaaaatga tatatgcgag tgtctaaata agtgcgtgtg ataatctccg
    29821 taaattttca atcgcttatt gatgtgtgca tttaaaattg gtaaaccttt tgaatatgga
    29881 aagctgttag cactctgctt gtgattaatc tatcgtatgt taaaagtgac atttaaatta
    29941 aatcattttt tatacatata tttcaacagg atttatcata ttggaaaata gcggtgcttc
    30001 tattggcatt ttactattga caggaatttc cctgtctttt gtctcctaaa gaagatcagc
    30061 tttgatttct aacttgatta gagtgggctt ggtactgttt atgaattatc agtatatact
    30121 ttctggagat caaaaaggaa aggaacagct aaagaggtgt atgtgttacc attagaggaa
    30181 tagaacatcg caagactcgt ttattcagtc attacgatac ctgttttatt tgggaaataa
    30241 ctataatatt aagtgaattt gtgatataat gaaaggtatg aattatcaag attatatatg
    30301 ggatttggga ggtaccttac tcgataatta cgagctttca acccaagctt ttgtccaaac
    30361 gctagctttt ttcagccttc caggagatca cgatgctgtt taccaaaaat taaaagaatc
    30421 aaccgctatc gctgtggcaa tgtttgctcc taatgagcca gaatttttgc atgtgtatag
    30481 gctaagagaa gcagacaaat tggcacaacc catttggtgt ttgggagcta aagaaatttt
    30541 ggaaaagata gcgacttcag gttcgcgaaa ttttttaatt tctcatcgag attgtcaggt
    30601 gaaccaactt ttagagcaag ctgggttgct aatctatttt acagaagtcg tgacagcttc
    30661 aaatggcttt gctcggaaac caaatcctga aagtttgttt tatttaaaag aaaagtatga
    30721 tattaatagt ggattagtaa ttggagatcg gctaattgat aaacaagcag gacaagcagc
    30781 gggctttaat accttgcttg ttgacggtag aaaaaaccta ttggagatag taacgtagat
    30841 gattgaagaa aataaacatt ttgaaaaaaa aatgcaagaa tacgatgcca gtcaaattca
    30901 ggttctagaa gggctggagg ctgtccgcat gcgtccaggg atgtatattg gctcgacagc
    30961 taaagagggt ttgcatcatt tagtctggga aattgttgac aactcaattg acgaagcatt
    31021 agcaggtttt gcctctcata ttaaagtctt tattgaagca gataattcca ttacagtagt
    31081 agatgatggc cgtggaattc cagttgacat ccaagccaag acaggacgtc ccgccgttga
    31141 aacagttttt acagtcttac acgcaggtgg taaatttggc ggaggcggct ataaggtttc
    31201 tggaggattg catggtgtag ggtcatctgt tgttaatgct ttatcaacac aattagatgt
    31261 gcgtgtttat aaaaacggcc aaattcatta ccaagaattt aaacgcgggg cagttgtagc
    31321 agatcttgag gtcattggaa ccactgatgt gactggaaca acggtacact ttacacccga
    31381 tccagaaatt tttaccgaaa cgactcagtt tgattacaat gttttagcaa aacgtattca
    31441 agagttagcc tttttgaatc gtggtttaaa aatttccatt acagataagc gctcaggtat
    31501 ggaacaagaa gaacatttcc attatgaagg tggaattggt tcttatgttg aatttttaaa
    31561 tgataaaaaa gatgttatct ttgaaacgcc catctataca gatggtgaat tagaaggtat
    31621 tgcagttgaa gtagccatgc aatacacgac tagctatcaa gaaacagtca tgagttttgc
    31681 taataatatt catactcatg aaggtggaac gcatgaacaa ggctttagag cggctcttac
    31741 tcgggtcatc aatgactacg ctaagaaaaa taaaattctt aaagaaaatg aggacaattt
    31801 gacaggagaa gatgttcgtg aaggtttgac ggcggtaatt tctgttaagc atccaaatcc
    31861 tcaatttgaa ggtcaaacca aaacaaaatt gggcaactca gaagtggtta agatcactaa
    31921 tcgtctcttt agtgaggcct ttcaacgttt tcttttggaa aacccacaag ttgctcgtaa
    31981 gattgtggaa aaagggattt tggcttctaa agctagaatt gcagctaagc gagcccgcga
    32041 agtcacccgc aaaaaatcag gcttagaaat ttcaaactta cctggaaaat tagcagactg
    32101 ttcgtcaaat gatgctaacc aaaacgaact tttcatcgtc gaaggagatt cagcgggtgg
    32161 gtcggccaaa tcaggtcgta accgagagtt tcaagctatc ttgcctattc gcggtaaaat
    32221 tttgaacgtg gaaaaagcaa ctatggataa gattcttgcc aacgaagaaa ttagaagtct
    32281 ttttaccgct atgggtacag gttttggtgc agattttgac gtgtcaaaag ctcgctacca
    32341 aaagctggtt atcatgaccg atgccgatgt ggatggcgct catattagaa ccttgctttt
    32401 aaccttgatt taccgcttta tgaggcctgt tctagaagct ggctatgttt atatcgccca
    32461 gccacctatt tatggtgtta aggtcggtag tgagattaaa gagtacattc agccaggtat
    32521 tgatcaagaa gaccaattaa aaacagctct tgaaaaatat agtattggtc gttcaaaacc
    32581 aactgttcaa cgttataaag gtcttgggga aatggatgac catcaacttt gggaaactac
    32641 tatggatcct gaaaatcgtt tgatggcgcg tgtgacagtt gatgatgccg cagaagcaga
    32701 taaagtattt gatatgttaa tgggagatcg tgttgaacca agacgtaatt tcattgagga
    32761 aaatgcggtt tatagtacac tggatattta gtcacctttt tagtttcaca aggaatctgc
    32821 taccaaagag gacgaattga ctaaaaatca tgatgcatga tgccaacagc tttgttagtt
    32881 agtacgtctt catcttgagg gacagcgtta atgcataggt attgagaccg ccccttaata
    32941 cctatgttag tccttattaa gaaagccttg tgagtcgctg gtacgagatt gttgaattag
    33001 tcaaatatat tttgaaatgc attttaaagt atgttataat cgtaatgtta tagtgtattt
    33061 taaataaaat tttaataaaa ggcgattgag gaatttcagt ttttagttat tactggaaca
    33121 atgttgataa atcgtgtcta aataataagg agattcagat gtcaagcgga attatcttgc
    33181 tgattgtggc tatagtccta ctagtgatta tcgcctatct agtgggtgtc atcatacgaa
    33241 agcgtaatga ttccctaatt accagtctag aagaacgaaa acaggccttg tttgctttgc
    33301 ctgttaatga tgaaattgaa gaagtaaagt cacttcattt gataggacag agccaaacct
    33361 cttttcggga atggaatcag aaatgggtag acttaactgt aaattcgttt gcagatattg
    33421 aaaatcatat ttttgaagct gaaaatttga atgatacgtt taattttatt cgcgccaagc
    33481 atgagattaa tagcgttgaa agtcagctta atcttgttga agaagatatc gcttctattc
    33541 gtgaggcact taatatctta aaagagcaag aagaaaaaaa tagtgctcgt gtcacccatg
    33601 ctcttgattt atatgaaaag ctccaagctt ctatttctga gaatgaagat aattttggtt
    33661 caactatgcc tgaaattgac aaacagatga aaaacattga aactgaattt tcacagtttg
    33721 tcgcactcaa ctcatcaggt gatcctgtag aagcttctga agttctggat agagcagaag
    33781 agcatactat tgctctaggg cagattacag agcagattcc agctattgtc gccaagttgg
    33841 aagatgattt tccagatcag ttagatgatt tggaaacagg atatcgccgt ttacttgaag
    33901 aaaactatca ttttccagaa aaaaacattg aggcacgttt tcaggagatt cgtgagtcta
    33961 ttcgtgccaa ttcatcagag ttagttacac ttgacttgga tcgtgctaga gaagaaaaca
    34021 cgcatattca agaacgcatt gattctcttt atgaagtctt tgagcgcgaa attgccgctt
    34081 ataaggtagc tgctaaaaat agtaagatgc tacctcgtta cctagcacat gtgaaacgta
    34141 ataatgaaca actcaaagat gaaattgcac gtttatcacg taaatatatt ctaagtgaaa
    34201 cggaaagtct gactgttaag gcatttgaga aggatattaa agagatcgaa gacagtacac
    34261 ttgctgttgc agaacaattt ggtttacaag aaaaaccatt ttcagaactg caagtgactt
    34321 ttgaacgcag tataaagacg cttactaatg tcgagtcagg tcaaatggat gtttttgcag
    34381 cagttaaaga tattgaaaaa attgaatcac aggcacgcca taaccttgat gtttatgtta
    34441 ctcagcttca tatgattaaa cgttatatgg aaaaacgtca cttgcctgga attccacaag
    34501 actttcttag tgctttcttt acaacgagtt cacagttgga agctttgatg gatgagctta
    34561 gtagaggacg gatcaatatt gaggcagtgt cacgtttgtc ggaagttgcg acggttgcta
    34621 ttgctaatct agaagattta acctatcaag tggttcaaaa tgctaccttg actgaacaat
    34681 tactacaata ttctaatcgc tatcgttctt ttgaagcagg agttcaaagt agttttgaac
    34741 atgctttgag acttttcgaa gtagaaaatg attatcaggc ttcttttgat gaaatttctt
    34801 atgctcttga aacagttgaa cctggggtaa cagatcggtt tgttaactct tatgaaaaaa
    34861 cacgagagca cattcgtttt tgatacttga aagtcgtgat attctcacga ctttttatta
    34921 gctttttgga ccaatacgtg tttttgacta gaatcttctt tttaatgatc aaacgaagaa
    34981 gtagtaacag tgttttttgg tattgagagg atagttattt tttcaaaaat cttttccaaa
    35041 aggatttggt tttttcagga ggttgtttgc taagttgatc attttttgtc tgctttaaga
    35101 tgacttctaa acgcgtttcc tttagattta ttgcgtggtc ggtatgaagg ataaacgcaa
    35161 aaggtgaagt catatgcgtt tctgtaataa tagtaacagg taaatggttt tcttttgcta
    35221 atttcatata gagtatttga agttgaggtg ccagcttgga agagattttt aagtgtaaag
    35281 gttgatagac taactgaagg ctcggtaatt ctcttttcaa atagtctttt actgttttag
    35341 tttgagcttc gtctaataaa gcacttaaaa cgactcgttc agcatacgtt cctagatagt
    35401 agcgttgctg ctcaggattt aatcttcttt ctccccaggc tccttttaat agcttatctt
    35461 ctaaattagt catagatacc ctcgcttctt tataatagtt tctttattat atcgatttct
    35521 acaataaaat accaatttga aaattttcgg taaatgtgtt aaaaaacaag aaagaaagtg
    35581 atttttcttg caaaaaacaa aggaagcttt tcatgagatt gctttttaag ttgattaaag
    35641 gcctagtttg tggtattatg aacatgtaaa atattaaaac tcataaggag agtattacaa
    35701 tgtcaattat tactgatgta tacgctcgcg aagtccttga ctcacgcggt aacccaacac
    35761 ttgaagtaga agtttataca gaatcaggtg cattcggacg tggtatggtt ccttcaggag
    35821 cttcaactgg tgaacatgaa gccgttgaac ttcgtgatgg tgacaaatct cgttaccttg
    35881 gtctaggtac tcaaaaagca gttgataacg ttaataacat tatcgctgag gcaatcatcg
    35941 gttacgatgt tcgcgatcaa caagctatcg accgtgcaat gatcgctctt gacggtactc
    36001 ctaacaaagg taaacttggt gctaatgcta ttcttggtgt ttctatcgct gttgctcgtg
    36061 cagctgctga ctatcttgaa gtgccacttt acacttacct tggcggattc aacactaaag
    36121 ttcttccaac tcctatgatg aacatcatca acggtggatc acactcagat gccccaattg
    36181 cattccaaga gttcatgatc atgccagttg gtgcacctac tttcaaagaa ggtcttcgtt
    36241 ggggtgctga agttttccac gctcttaaga aaattcttaa agaacgtgga cttgttacag
    36301 ctgtaggtga cgaaggtgga tttgctccta aatttgaagg aactgaagac ggtgtagaaa
    36361 ctatccttaa agctattgaa gcagctggtt acgaagctgg tgaaaacggc attatgatcg
    36421 gttttgactg tgcatcatca gaattctacg acaaagaacg taaagtttac gactacacta
    36481 aatttgaagg tgaaggcgct gctgttcgta catctgcaga acaagttgat taccttgaag
    36541 agttggttaa caaataccca attattacta tcgaagatgg tatggatgaa aacgactggg
    36601 atggttggaa agttcttact gaacgcctag gcaaacgtgt tcaattggtt ggtgacgact
    36661 tcttcgttac aaacactgaa taccttgctc gtggtatcaa agaaaatgca gctaactcaa
    36721 tccttatcaa agttaaccaa atcggtactt tgactgaaac gtttgaagct atcgaaatgg
    36781 ctaaagaagc tggatatact gccgttgtat cacaccgttc aggtgaaact gaagattcaa
    36841 caatcgctga catcgcagtt gcaacaaacg ctggccaaat caaaacaggt tcattgtcac
    36901 gtacagaccg tattgctaaa tacaaccaat tacttcgtat cgaagatcaa cttggtgaag
    36961 ttgctcaata caaaggtatc aaatcattct ataacttaaa aaaatagacg gtaagacaga
    37021 aatcggtcat tcgtagaatg cgatttcgtc gtcgcacccc cgtcaggctg actaggttgg
    37081 tcacgacaaa aggagtgaac atccagtcag ctacgacgca aaagttaaag attaaagaat
    37141 agaaagcttg ggaaaatccc aagctttttg ctttctaaat agtaaaaatt tactgtgggc
    37201 taatttatcc tgagctaatg tattagcgag gtaaagttat tgaggaaggc ttggatttgt
    37261 actgcctcta aaaagttgga cactatattt taaatacagg actaagtcct tttataccaa
    37321 ttgtcaagca catcaaggtt ttaactgaaa aaatgtttta gacaatagcc taggtttagc
    37381 tgttgtctta cttgttgtgc tctgattagc ataaaaaagt gtacaagaaa aaaaccttgc
    37441 ctagtatttt taagatcaag gtgttacaat gatagagcaa caacgacttt aatgattttg
    37501 ggtcgaagcg taattgtaaa gtttgttatg cgttatgagg taaagcattg tctgacaagg
    37561 caatgtctag aggcaatcgt atgtggcttg gttgaagctg tttgcgattg cctttttcgt
    37621 ttctcataga agtctgcgat atgacaagga ttggtatgac tagcaaaagc aatgtcatgg
    37681 atacacttga aaagaatctt tgaagcataa ggattcccac gtttggtaat gtgctcttga
    37741 gctagatagt tgccagactc ataatgtcta aagtcaatcc caatgaaagc attaatttga
    37801 ttagcagatt gaaaacgacg aatagcaccg agttcgccaa taatacttgt tgcagttgtt
    37861 tctgcgatac ttggaataga gcgtagaatc ttgtcttctg gtaaaggttg agccaacgcc
    37921 accatcttat caaaaatagc ttgacgacgt tcagaaagtc taagtaattc tttggcatag
    37981 tagcgaacct cttctatcat cggagaggtt ttcgtaacag taaaataaga ctgcttggct
    38041 aatgagacaa gtttttccac taagtagatc ccacgcttgt cagagatacg tttagaggta
    38101 gattgacgga tgatagctga caatgctttg tcagctagct ccagtacaaa gctattacat
    38161 ggaaaagccg ttaccacatt ccagtattac tcaccagttg gtgcaaataa gatgtgttcc
    38221 agttcaggaa aagtgacttg taagatcttg tgaaggcgat tcttaactct cgaacaatat
    38281 tatcctcagt caatttttga tagaagcgac tcaagtcacg tagcagctga taaacttctt
    38341 cctgaacgta agttggtttt ctaactagaa taaattgaga ctgagccaat ttttcagtgt
    38401 ctaacttatc cgctttacgc acccgtagac tatctagttt tctaaaggat tgagttgtgt
    38461 ataagcgtag ccatttttct caaggaagga ctgaagccga cgagaataga ctcctgttgt
    38521 gatggtctcc cttttgcctt ttaaacaata gtatagccgt ttttcaagat gttattaatg
    38581 attttttcct ttattctaga agaataagtc tgatactgtt tcttcttaat aatctcaatg
    38641 ccctaacagt ccatcagtcg aacggtattt ctcaaattac tgcagttgac tcttgcgttc
    38701 agttccctca ccttgtggat gccgtttaga acaatattag cttgctgaat tagcttgctg
    38761 agccttttgg taatgtgaac aaagcatctt tatcactcaa ggagaagaca ggttcttgtg
    38821 gtaagtgtcc aacaaggtca cgataaactc atcgtaccgt ttttggcgga agtctagaaa
    38881 tgccttatga atgtaagcct cagaaacgtc tctaaaacta aacaagaaat acaagaacaa
    38941 atgcccttga ttctggtaca aatgctgtat caggttcagg tattgcctat ctgcgataat
    39001 gacactctgc ttttccttaa caatatcttt gaaaggaatg atttcgacat tgacaggcat
    39061 aaccgttgca atagctggcg ggaagtaaat cgagagtgca ttggtcatca aatccagcgc
    39121 agcagtgtta ttggtcaaaa gatagacagg tatcggtggc aaatatgttt ttaggagttc
    39181 ctctaactgt atcgtgaaga gcagtaaatg ataggacggc agtttcccca cgatgccttc
    39241 tgcgctcatc cattcctcaa cgatggtttt tagagtattg tagaagctct tgtttcgttt
    39301 ttgctgtctt ctgaggtaat ggaacaagtg tatatcaggt aacaaaactt gaagattact
    39361 gataaataga cgtgagaagg aaaccaaaat ccgtttgaaa ggaagggagt ttaggatatt
    39421 attgcctagt agtggacgca gggatgcttt caaggttttt ccatttgttt ttgtcatcac
    39481 caactcaaac aattctttca cttttttagg tgtccattta tccttatgga aagaattatt
    39541 ggtggtgcaa aaacataaga aaatataatc caaatcctca tcagttaaat caggatggta
    39601 ccattttccg atgatttcct gactggtcat tttgatatct cgatagatag acagtttttt
    39661 caagtgtttg aatgcatctg tctgagggat ccccaagtta tcatgtcgcc gtttccatat
    39721 aagggatatc agaatggcaa agtatgaaaa ttcataaggg gctttactgg catcattaag
    39781 tgtgatggct tgattggagt ttataatcag cgaaaccact ttattcattg atgttttatc
    39841 aaaatcataa atggtcatac caaactgata gtgcaacaag gcgatcagaa agcggatacg
    39901 gtattctggt ccaacaatat ggttttgccg aacgtttaaa cctacctttt tcaagtattt
    39961 ccgacaattg gtcctgatac ggtaagcggt ggcttgggaa atatactctc gtttaatgaa
    40021 gtcgtgtagg ctcagctctc ctggttcaat taaaaaacat aacatcttta agaatatcga
    40081 ggtttgatag agtttcttaa aataaggcat tttaagttgg taggtcttat aggggataga
    40141 aacagtgtcg tcacaaatgg ttaaggtgag ggtgtcttct aaatccttaa tcatttcaaa
    40201 gagtaagttg tagacacgtt gttttttgag ttcggtttcc tgacacaact ggtctatgga
    40261 gacttcggaa acgtctaaca aaatgttgag cagtgataat ttatcttgta atttggtttc
    40321 tagatgaagg tgtaacataa ctgaactcct ttcattttta tagcagaaac tcgtatttat
    40381 ttaattatca cataattaaa taaaacagtc aaataattca atggtgacgc gtgtttaagg
    40441 agtattgaga atgtcagata taagcaaaat gcagctgaat attcttaatt ttaggtatga
    40501 aaaagcgtcg ggattgataa aaaatctatt gaattgggta aatttaagtt gatataataa
    40561 gttgattaga aaaattaata attcatttaa attctaatca gctgtaacgt tccggggcgt
    40621 gttaaactgt ctgaacatag aaaatacagg tttctacggt gactaaaata agctaaggag
    40681 gattccttaa attctgtgat aggaacgtaa actctctaca agagtagagc acgtcatcag
    40741 tcagtctggg tgtctactgg ctgacaacgt caaataagat aggcatgaaa aaataagggg
    40801 gaatacagaa tgtttttaaa acaccaagat gtgaaacaaa aaaattggag aatgcgtaag
    40861 gtcaaaaaag tctttgttag ttcatgtatg cttttaacag tgggcctcgg agttgccgta
    40921 ctactgcatt cagccaatct aatggcgtga tggtcgtaaa ggctgcggaa gctgaagaac
    40981 tgccagacaa tttaattgat tttaaaggaa cttgggaagt atctgccgat ggctcttctg
    41041 gtcgcttcta ttcagacggt gctacaggtc aatataagtt tcaccttatt ccagcttcag
    41101 atgttaaaaa tccgggatgg catgaacata ataaagttaa agatagctat gtcaaaataa
    41161 ccaaagagtc tattgcagct agatatacaa ataaaacgaa acctccatat tctgtagcgt
    41221 ttaaggtcaa cactaaatct ttaataaaag atcatgacta taagataact tttgagcaag
    41281 gttcaattgc gtcgggaata acagttgatt atcgtatagg cagtgctttt aataaaacta
    41341 aagatgattc tttcaacatt tcagatgaga gtaaatatgc ttctaaggta acaatagaag
    41401 gtgaggaaag aggttttata aataggaaac caggaactaa aacaatatct tttagagctg
    41461 tagaaaaagg aactaaatct cttgtattgt tatctaaagt taatgaaaaa acccaagtag
    41521 atcttgatgt cgagtttaaa aacttcaaaa ttattgatgt taccaaccca agccaattag
    41581 ataaaggagt tgcttacgtt ggaaacaaaa atgtagaatt gacattaaaa tcagacgatg
    41641 gaagaacaaa cttcgaaggt gatgagataa gcctttttaa gcctaatgga gatttgttaa
    41701 agaaaattga agttaaagaa ggacaaaaaa atcctatatc aataacttta tctgaagatc
    41761 aagccaaatc tttaaaaaac aaagaaaagt taaaagtaag cattaaacaa aagcaatcaa
    41821 aaaagacttc taaagacttt ttctttgagg taggaattga tcctaaagtt aaagcaaagc
    41881 aacaagaaaa actcttagaa ctagacaaag taaaaaaaca aattgaagac tcaatcaatg
    41941 gtgatgcatg gctaccagaa aaatcggaag gcgaaaaacc agtacaaaat actaataaag
    42001 aattacagtt acaagaactg aacaagaagt atcagatggc caaagaggca attgaaagtg
    42061 ctacaactct tgacgatgtt gaaactcagt ttgataaata cacaaaagtt ggagataaag
    42121 ataaataccc ggattcttta cgtaaccaat atactcgagg agataaagat aaagaaatag
    42181 aaaaagctaa aaaatcactg ggtgatttaa gcgataaagt gaatggaaag attgaagaag
    42241 ataagtggct atctgacgaa gttaaaaaaa aacaacagca agaattagaa gcgcgtaaac
    42301 aaaaagtaaa cgactctctt aaaggatctg atagtttaaa atcattgaga gaaacagtgg
    42361 aaaaagcttc ttctaaaaat cagaaaaaac cagaatcttt tgaagatgtt tatgttcctg
    42421 gaaatgaaga aactgaaaaa acaaaagttc gtgatatact tcaaaaaact tatcaaaaaa
    42481 cagagcaaaa tattgaaaca gatccatggt tgagtccgga acagaaaaaa gctcaaaaag
    42541 aaaacgctaa agcaagatta gatgcaggtt tgaaagctgt cgaaactaca gaaagcttag
    42601 ataaacttaa agaagttgag agtgacttcc ttgataaaga aaaaaatcca gattcaattc
    42661 caaatcaaca taaagctgga actcctgaag ttgctcgcaa aactttctta gataactttg
    42721 aaaaagaagc caaaaaagag attgaatcta ttgataaaga tgatacctta acggcaaatg
    42781 ctaaacaagt agctaaagat aaagtggcac aacagctaca agaagctaca gctaaagtag
    42841 agaaagcaca gtcgtttgat gatttgaaga acattcaaga taagttcaat tctgaattac
    42901 cacacacacc aggtaagccg cttaaagatc aacaatctga tgctattgca gaattagaga
    42961 agaagcagca agaaattgaa aaagctattg agggtgataa aacattacca agagacgaaa
    43021 aagagaaaca aattgctgac tctaaggaac gcttaaaatc tgacacgcaa aaagttaaag
    43081 atgctaaaaa tgctgatgct attaaaaaag catttgaaga agggaaagtg gatattcctc
    43141 aagcacatat cccaggtgat ttgaacaagg ataaagaaaa acttcttgca gagttgaagc
    43201 aaaaagcaga tgatactgaa aaagcgattg attccgataa aactttgaca gcgaaagaaa
    43261 aagaagagca aaaagcgaaa acaaaagaag aacttcaaaa agcaacagaa gctgttggag
    43321 caattgacaa tcgtgaagaa ctagataaaa aagtcccaga acttaagaaa gctattgaag
    43381 acactcacgt taaaggtaat cttgaaggta ttaagaataa ggctattgaa gatcttaaaa
    43441 aagctcatac tgaaacagtt gctaaaataa atggtgatga tacccttgac aaagctacta
    43501 aagaagctca agtgaaagaa gctgacaaag ctttggcagc aggtaaagaa gctatcacta
    43561 aagcagatga tgctgataaa gtaagtacag ctgttacaga gcacacacca aaaattaaag
    43621 cagcacataa aactggtgat cttaaaaaag ctcaagtaga tgctaacaca gctcttgaca
    43681 aagcagctga aaaagaacgt gaagaaatca ataatgatat cacattaacg gcaaaagaca
    43741 aagaacaaca actgaaagaa gttgagacag ctcttactaa agctaaagat aacgtgaaag
    43801 ctgctaagac agcagacgct atcaatgacg cacgtgataa aggcgtagca acaattgatg
    43861 ccgttcataa agcaggtcaa gacttaggtg ctcgtaagtc aggtcaagtc gctaaacttg
    43921 aagaagcagc taaagcaacg aaagacaaga tttcagctga tccaactttg acaagcaaag
    43981 aaaaagaaga gcaatctaaa gctgttaacg ctgaacttaa gaaagcgata gaagctgtta
    44041 acgcagctga cacagctgac aaggttgacg atgctcttgg taaaggtgtc acagacatca
    44101 agaaccaaca taagacaggt gaccctgtcg tagctcgtcg tgaggctcat ggtaagcaac
    44161 ttgatagagt cgctcaagaa actaaagatg cgattgaaaa agaccctact ttgacgactg
    44221 aagaaaaagc taaacaagtt aaagacgtag atgccgctaa agaaagaggc atggctaagc
    44281 ttactgaagc taagaatgct gatgagttag acaaagctta cggtgaaggt gttacagaca
    44341 tcaagaacca acacaagtca ggtgacccta tcgaagctcg tcgtggatta cacaacaagt
    44401 caatcgacga agtggcgcaa gcaactaagg acgctatcac agcagatacg actttgactg
    44461 aagctgaaaa agaaacacaa cgtggcaatg ttgataaaga agcaactaaa gctaaagaag
    44521 aacttgctaa ggctaaagat gctgatgctt tagacaaagc gtatggtgac ggtgtaacca
    44581 gcatcaagaa ccaacacaag tcaggtaaag gtcttgacgt tcgtaaagat gagcacaaga
    44641 aagctcttga agctgttgct aaacgtgtca ctgctgaaat tgaggctgat ccaaccttaa
    44701 caccagaagt gagagaacaa caaaaagcag aggttcaaaa agagcttgaa cttgcgactg
    44761 ataagattgc tgaagctaaa gatgcagatg aagcagacaa agcttacggt gacggtgtca
    44821 cagcgatcga aaatgcccac gttattggta aaggtatcga agctcgtaaa gaccttgcta
    44881 agaaagacct tgctgaagct gctgctaaga caaaagctct cattattgaa gacaaaacgc
    44941 ttactgatga tcaacgtaaa gaacagttat taggtgttga tacagagtat gctaaaggta
    45001 tcgagaatat tgatgcagct aaggatgctg caggtgttga taaagcatat agtgacggtg
    45061 ttcgtgacat cctggcacag tacaaagaag gtcaaaacct taatgatcgt cgtaatgctg
    45121 ccaaagaatt tcttcttaaa gaagcagaca aagtgacgaa actaatcaat gatgatccaa
    45181 ccttgactca tgaccaaaaa gttgatcaaa tcaacaaagt tgaacaagct aagttagacg
    45241 caatcaagtc tgttgatgat gctcaaacag ctgatgctat caatgatgct cttggtaagg
    45301 gtattgaaaa catcaacaac caataccaac atggcgatgg cgttgatgtt cgtaaagcga
    45361 ctgccaaaga cgatcttgaa aaagaagctg ctaaagtgaa agctcttatt gctaaggatc
    45421 caaccttgac tcaagctgat aaagacaagc aaacagcagc ggttgacgca gctaagaaca
    45481 cagcaattgc agcggttgat aaagcgacaa cagcagatgg tgttaaccaa gaacttggta
    45541 aaggcatcac agctatcaac aaagcttacc gtccaggtga agctgttaaa gcacgtaaag
    45601 aagccgctaa agctgatctt gaaaaagaag ctgctaaagt gaaagctctt attgctaagg
    45661 atccgacctt aactcaagct gctaaagaca aacaaacagc agcggttgac gcagctaaga
    45721 atacagcaat tgtagcggtt gataaagcga caacagctga aggcattaac caagaacttg
    45781 gtaaaggcat cacagctatc aataaagctt accgtccagg tgaaggtgtt aaagcacgta
    45841 aagaagccgc taaggctgat cttgaaagag aagctgctaa ggttcgtgaa gctatcgcta
    45901 acgacccaac cttaacaaaa gctgataaag ctaaacaaac agaagctgtt gctaaagctc
    45961 ttaaagctgc tatcgcagcg gttgataaag cgacaacagc tgaaggcatt aaccaagaac
    46021 ttggtaaagg catcacagct atcaacaaag cttaccgtcc aggtgaaggt gttgaagcac
    46081 ataaagaagc tgctaaagct aatcttgaaa aagtagctaa agaaactaaa gctcttattt
    46141 caggagaccg ttacttgagc gaaactgaaa aagcagtcca aaaacaagct gttgagcaag
    46201 ctcttgcgaa agcacttggt caagttgagg ctgctaagac agttgaagct gttaagttgg
    46261 cagaaaacct tggtactgta gctatccgtt cagcatatgt tgctggttta gctaaagata
    46321 ctgatcaagc aacagctgct cttaacgaag cgaaacaagc tgctattgaa gctcttaaac
    46381 aagctgcggc agaaacactt gctaagatta caactgatgc taaattgact gaagctcaaa
    46441 aagctgaaca atcagaaaat gtatcattag cgcttaagac ggctattgcg actgttcgtt
    46501 cagcacaatc tattgcgtct gtgaaagaag caatagataa aggtattact gctatccgtg
    46561 cagcctatgt gcctaataag gcagtcgcaa aatcatcgtc agcgaaccat cttccaaaat
    46621 caggtgatgc aaactcaatt gttcttgttg gcttaggagt tatgtctctt cttttaggta
    46681 tggtgcttta tagcaagaaa aaagaaagta aagactaaga acctggcttt atccaaattg
    46741 ctttctacta tattttcaaa ccaaggtgat tttgaaatca ccttggtttt ttggatcgta
    46801 agggcccaag aacggagtgt attgaaaaca tttcaaagtt caattgaaaa ggcaacagtt
    46861 ttctgtacag aatttataag gcgttttttg ttcaaacgat gactcattta gttatgggtg
    46921 tttgataatt aagattactg tgaaggtgat ggtagttcca ccagtggact tcctccttgg
    46981 ttttaagggt taattgttct agtgaataaa aggtttcttg gtcgcgcagt agggatcaag
    47041 cgagcagata aaatgcctat tttaaatcag ttcgagcgaa ttttcgtgct acaagctcaa
    47101 aagaggctac taaagtatta gaatagttta tctctgagtg aagttcttgt tataagaaga
    47161 tgatgttatc gtttgaaacg accaaaaaat ctcctaactt ttactaattt ctacatgaaa
    47221 tctagtccag tatttactct acaaccttga ttgattcgct taataaagac atttctactt
    47281 gat
//
LOCUS       DATUYR010000008        46341 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.008, whole genome shotgun sequence.
ACCESSION   DATUYR010000008 DATUYR010000000
VERSION     DATUYR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46341
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.008"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            46..118
                     /locus_tag="VCI33_000374"
     tRNA            46..118
                     /locus_tag="VCI33_000374"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:79..81,aa:Phe,seq:gaa)
     gene            138..218
                     /locus_tag="VCI33_000375"
     tRNA            138..218
                     /locus_tag="VCI33_000375"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:172..174,aa:Tyr,seq:gta)
     gene            225..295
                     /locus_tag="VCI33_000376"
     tRNA            225..295
                     /locus_tag="VCI33_000376"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Trp,seq:cca)
     gene            307..379
                     /locus_tag="VCI33_000377"
     tRNA            307..379
                     /locus_tag="VCI33_000377"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:His,seq:gtg)
     gene            388..459
                     /locus_tag="VCI33_000378"
     tRNA            388..459
                     /locus_tag="VCI33_000378"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:420..422,aa:Gln,seq:ttg)
     gene            479..562
                     /locus_tag="VCI33_000379"
     tRNA            479..562
                     /locus_tag="VCI33_000379"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:513..515,aa:Leu,seq:caa)
     gene            822..1007
                     /locus_tag="VCI33_000380"
     CDS             822..1007
                     /locus_tag="VCI33_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831324.1"
                     /translation="MSFEIFTLIIGWKLVGYRVMECGSSFKQGDYRLVVHICLLNVRD
                     EMLSQQGHEDKADWPEP"
     gene            1645..1953
                     /locus_tag="VCI33_000381"
     CDS             1645..1953
                     /locus_tag="VCI33_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEP2831325.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDVPYCLLSGRAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            2008..2220
                     /locus_tag="VCI33_000382"
     CDS             2008..2220
                     /locus_tag="VCI33_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEP2831326.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATTLMTGSGPTVFALCQTEKQTIIV"
     gene            2330..2773
                     /locus_tag="VCI33_000383"
     CDS             2330..2773
                     /locus_tag="VCI33_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEP2831327.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2777..3496
                     /locus_tag="VCI33_000384"
     CDS             2777..3496
                     /locus_tag="VCI33_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP2831328.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            3489..4304
                     /locus_tag="VCI33_000385"
     CDS             3489..4304
                     /locus_tag="VCI33_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP2831329.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4344..4727)
                     /locus_tag="VCI33_000386"
     CDS             complement(4344..4727)
                     /locus_tag="VCI33_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP2831330.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWESITINQENWDLTPFLKHL"
     gene            complement(4778..6034)
                     /locus_tag="VCI33_000387"
     CDS             complement(4778..6034)
                     /locus_tag="VCI33_000387"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEP2831331.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLFLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6126..8438
                     /locus_tag="VCI33_000388"
     CDS             6126..8438
                     /locus_tag="VCI33_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP2831332.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTIRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADSNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8702..12268
                     /gene="rpoB"
                     /locus_tag="VCI33_000389"
     CDS             8702..12268
                     /gene="rpoB"
                     /locus_tag="VCI33_000389"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP2831333.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12359..16000
                     /gene="rpoC"
                     /locus_tag="VCI33_000390"
     CDS             12359..16000
                     /gene="rpoC"
                     /locus_tag="VCI33_000390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEP2831334.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDRLDINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            16152..16517
                     /locus_tag="VCI33_000391"
     CDS             16152..16517
                     /locus_tag="VCI33_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEP2831335.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            16610..17548
                     /gene="tadA"
                     /locus_tag="VCI33_000392"
     CDS             16610..17548
                     /gene="tadA"
                     /locus_tag="VCI33_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEP2831336.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYVRLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            17532..18518
                     /locus_tag="VCI33_000393"
     CDS             17532..18518
                     /locus_tag="VCI33_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEP2831337.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQDLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            18520..18846
                     /locus_tag="VCI33_000394"
     CDS             18520..18846
                     /locus_tag="VCI33_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEP2831338.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            18821..19249
                     /locus_tag="VCI33_000395"
     CDS             18821..19249
                     /locus_tag="VCI33_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEP2831339.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            19254..19490
                     /locus_tag="VCI33_000396"
     CDS             19254..19490
                     /locus_tag="VCI33_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEP2831340.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEITLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            19483..19917
                     /locus_tag="VCI33_000397"
     CDS             19483..19917
                     /locus_tag="VCI33_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEP2831341.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            19901..20227
                     /locus_tag="VCI33_000398"
     CDS             19901..20227
                     /locus_tag="VCI33_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEP2831342.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            20325..21278
                     /locus_tag="VCI33_000399"
     CDS             20325..21278
                     /locus_tag="VCI33_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP2831343.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVTLITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            21337..22533
                     /locus_tag="VCI33_000400"
     CDS             21337..22533
                     /locus_tag="VCI33_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEP2831344.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEEVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGEISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            22720..23028
                     /locus_tag="VCI33_000401"
     CDS             22720..23028
                     /locus_tag="VCI33_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831345.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLALIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(23111..23881)
                     /locus_tag="VCI33_000402"
     CDS             complement(23111..23881)
                     /locus_tag="VCI33_000402"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEP2831346.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRNLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(23929..24996)
                     /gene="pepA"
                     /locus_tag="VCI33_000403"
     CDS             complement(23929..24996)
                     /gene="pepA"
                     /locus_tag="VCI33_000403"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEP2831347.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPNIENIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            25452..25745
                     /locus_tag="VCI33_000404"
     CDS             25452..25745
                     /locus_tag="VCI33_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEP2831348.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            25742..26059
                     /locus_tag="VCI33_000405"
     CDS             25742..26059
                     /locus_tag="VCI33_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP2831349.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFICVNRDQFIEVAQKWNIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            26077..26703
                     /locus_tag="VCI33_000406"
     CDS             26077..26703
                     /locus_tag="VCI33_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEP2831350.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEVSSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPTVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELVLPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            26855..27250
                     /locus_tag="VCI33_000407"
     CDS             26855..27250
                     /locus_tag="VCI33_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP2831351.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISIVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(27511..28152)
                     /locus_tag="VCI33_000408"
     CDS             complement(27511..28152)
                     /locus_tag="VCI33_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEP2831352.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(28172..29149)
                     /gene="dusB"
                     /locus_tag="VCI33_000409"
     CDS             complement(28172..29149)
                     /gene="dusB"
                     /locus_tag="VCI33_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEP2831353.1"
                     /translation="MTKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFKTVR"
     gene            complement(29136..30008)
                     /gene="hslO"
                     /locus_tag="VCI33_000410"
     CDS             complement(29136..30008)
                     /gene="hslO"
                     /locus_tag="VCI33_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEP2831354.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNENDLEALINDKA"
     gene            complement(30155..31648)
                     /gene="rofA"
                     /locus_tag="VCI33_000411"
     CDS             complement(30155..31648)
                     /gene="rofA"
                     /locus_tag="VCI33_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEP2831355.1"
                     /translation="MIEKYLESSIESKCQLIILFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRDLNHKHFHLFCHYIEQSLRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVHHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
     gene            31915..33585
                     /gene="sfbI"
                     /locus_tag="VCI33_000412"
     CDS             31915..33585
                     /gene="sfbI"
                     /locus_tag="VCI33_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
                     /protein_id="HEP2831356.1"
                     /translation="MNNKIFLNKEAGFLAHTKRKRRFAVTLVGVFFMLLACAGAIGFG
                     QVAYAADEKTVPNFKSPDPDYPWYGYDAYTGAFLRYHDLRVNLNGSRSYQVYCFNIKK
                     NYPRPFTSSDKKWYKRLEGTAETFKVHAMAPRVGGEELTKKLRSVMYNGYPNDGNNIM
                     KGLEPSNAIEVTQYAVWYYSDKSQYEIETLWKSEAKEGKISESQVTLMREALRKLISS
                     DEDLVKQVPSNFKLSTFESSDKSYQNLLSAEFVPDDPPKPGDTSEHNPKTPELDGSPI
                     PEDPKHPDDNLEPTLPPVMLDGEEVPEVPSESLEPALPPLMPELDGEEVPEVPSESLE
                     PALPPLMPELDGQEVPEKPSVDLPIEVPRYEFNNKDQSPLAGESGETEYITEVYGNQQ
                     NPVDIDKKLPNETGFSGNMVETEDTKEPEVLMGGQSESVEFTKDTQTGMSGQTTPQVE
                     TEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIVEDTRPKLVFHFDNNEPKVEE
                     NREKPTKNITPILPATGDIGNVLAFLGILILSVLSIFSLLKNKQNNKV"
     gene            33873..34721
                     /locus_tag="VCI33_000413"
     CDS             33873..34721
                     /locus_tag="VCI33_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEP2831357.1"
                     /translation="MTERLKNLGILLLFLLGTAIFLYPTLSSQWNAYRDRQLLSTYHK
                     QVTQKKPSEMEEVWQKAKAYNARLGIQPVPDAFSFRDGIHDKNYESLLQIENNDIMGY
                     VEVPSIKVTLPIYHYTTDEVLTKGAGHLVGSALPVGGDGTHTVISAHRGLPSAEMFTN
                     LNLVKRGDTFYFRVLNKVLAYKVDQILTVEPDQVTSLSGVMGKDYATLVTCTPYGVNT
                     KRLLVRGHRIAYHYKKYQQAKKAMKLVDKSRMWTEVVCAAFGVVIAIILVFMYSKMTD
                     KKVIKN"
     gene            35007..37250
                     /locus_tag="VCI33_000414"
     CDS             35007..37250
                     /locus_tag="VCI33_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEP2831358.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMLLVLIGFEGKV
                     RAAHELVEVPVPIFHNPDPQSDYQWYGYEAYTGGYPKYDLFKTYYHDLRVNLHGSKSY
                     QVYCFNVHKHYPRSSQSFDRKWYKKLDGTAENFDSLAMEPRVRKEELTKKLRAVMYNA
                     YPNDANGIMKDLEPLNAIKVTQEAVWYYSDSAQINPDESFKTEAQSNGINDQQLGLMR
                     KALKELIDPNLGSKYSNKTPSGYRLNVFESHDKTFQNLLSAEYVPDTPPKPGEEPPAK
                     TEKTSVIIRKYAEGDYSKLLEGATLRLTGEDIPDFQEKVFQSNGTGEKIELSNGTYTL
                     TETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVGSPYTIEAYNDFDEFGL
                     LSTQNYAKFYYGKNYDGSSQIVYCFNANLKSPPDSEDHGATINPDFTTGDIRYSHIAG
                     SDLIKYANTARDEDPQLFLKHVKKVIENGYHKKGQAIPYNGLTEAQFRAATQLAIYYF
                     TDSVDLTKDRLKDFHGFGDMNDQTLGVAKKIVEYALSDEDSKLTNLDFFVPNNSKYQS
                     LIGTEYHPDDLVDVIRMEDKKQEVIPVIHSLTVKKTVVGELGDKTKGFQFELELKDKT
                     GQPIVDALKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETEAKDYIVTVD
                     NKVSQEAQSASENVTADKEVTFENRKDLVPPTGFTTDGGTYLWLLLLVPFGLLVWFFG
                     RKGLKND"
     gene            37243..37764
                     /gene="lepB"
                     /locus_tag="VCI33_000415"
     CDS             37243..37764
                     /gene="lepB"
                     /locus_tag="VCI33_000415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP2831359.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVNFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            37786..38823
                     /locus_tag="VCI33_000416"
     CDS             37786..38823
                     /locus_tag="VCI33_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEP2831360.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKSETAGVSENAKLIVKKT
                     FDSYTDNEVLMPKADYTFKVEADSSATDKTKDGLEIKPGVTEGLTTEQTIAYDNSVKP
                     SDKSKTATFDFSTVKFPEVGVYRYTVSEIDSKVSGIKYDTKTWIVDVYVVNDGNGGFK
                     ARYIVSKEKGQNDKKPVVFENSFKTTSLKVEKQVTGNTGELKKDFNFTLTINPNDNFV
                     AGQVIKLEKGGIKADVKIGEPYKFALKNGEKVTLSKLPVGVTYSIIEDEADKDGYTTN
                     AKITDGTAAPVEYKLGNQQLADESADEIVVTNNRDTQVPTGVVGTLAPFAVLSIVAIG
                     GVIYITKRKKA"
     gene            38839..39564
                     /gene="srtB"
                     /locus_tag="VCI33_000417"
     CDS             38839..39564
                     /gene="srtB"
                     /locus_tag="VCI33_000417"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEP2831361.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNNFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            39581..40168
                     /locus_tag="VCI33_000418"
     CDS             39581..40168
                     /locus_tag="VCI33_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEP2831362.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSIALESIDAMKTIEEITIAGSGKASFSPLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDETLAAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPDIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSRL"
     gene            complement(40327..41532)
                     /locus_tag="VCI33_000419"
     CDS             complement(40327..41532)
                     /locus_tag="VCI33_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEP2831363.1"
                     /translation="MVIFDLKYVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNVSLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            41917..45411
                     /locus_tag="VCI33_000420"
     CDS             41917..45411
                     /locus_tag="VCI33_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP2831364.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGSFEIKKVDQNNKPLSGATFSLTPKDGKGKPVQTFTSSEEGIIDAQNLQPGTYTLK
                     EDKAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLEKSTMSV
                     FSKYGGTEVTSGAADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDKRLNPKGI
                     SQDIPKIIYDSENSPLAIGKYDAKTHQLTYTFTNYIAGLDKVQLSAELSLFLENKVVL
                     ENQTISDFKSTIGDQEIKYGGTVNVLYGNASGQDNNYVTNGLSNVGGSIESYNTQTGD
                     FTWYVYVNPNRTNIPYATMNLWGFGRLVKSNNSTSKSEDSATSSAQLEKVEIYEVKED
                     YTLPTSYGVDLTKLNLRKDLEAKLPQDSTQNSNKRLRIDFGDKLQNKAFVVKITGKTD
                     QSGKPLVVQSNLASFNNETAVTYGNPVNNVYFQNEIALSPSKGSGSGTSEFTKPSITV
                     ANLKRVAQLRFKKMSTDNVPLPEAAFELRSSNGNSQKVEAKSNTQGEVHFKDLTSGTY
                     DLYEKQAPKGYQQVTEKLATVTVDTTKPAEEMVTWGSPHSSVKVEDSKEVTIVNHKET
                     LTFSGKKIWENDRPDQRPAKIQVQLLQNGQKMSDKVQEVTKDNDWSYHFKDLPKYDDR
                     NQEYKYSVEEVKVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKI
                     IDEDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKN
                     LKKYNGTGNDIIYSVREVTVPTGYDVTYSANDIINTKREVITQQGPKLEIEETLPLES
                     GASGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATM
                     ELRDSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQVTV
                     NGKATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDLIIGG
                     QGEVVDTTEDTQSGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMHGDSGC
                     KTEVEDTKLVQSFHFDNKESESNSEIPKKDKPKSNTSLPATGEKQHNMFFWMVTSCSL
                     ISSVFVISLKTKKRLSSC"
     gene            complement(45677..46294)
                     /locus_tag="VCI33_000421"
                     /pseudo
     CDS             complement(45677..46294)
                     /locus_tag="VCI33_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055900.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        14473 a         8111 c         9846 g        13911 t
ORIGIN      
        1 gtaggttcaa atcctgctcc cgcaatacat aagtatgtag ttgttggctc ggtagctcag
       61 ttggtagagc aatggattga agctccatgt gtcggcggtt cgattccgtc tcgcgccatt
      121 aaaatttaat attttacgga aagatagcga agaggctaaa cgcggcggac tgtaaatccg
      181 ctccttcggg ttcgggggtt cgaatccctc tctttccatc ctaaacgggc atagtttaaa
      241 ggtagaacta aggtctccaa aaccttcagt gtgggttcga ttcctactgc ccgtgttaga
      301 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      361 tcgattccca tcactcgcct attttattgg ggtatagcca agcggtaagg caagggactt
      421 tgactccctc atgcgttggt tcgaatccag ctaccccagt ttaaatttta tatttaaagc
      481 cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc agtgtccgca aggacgtgcc
      541 ggttcgaccc cggccgccgg tatacttgaa aaacaaggtt tacaaacctt gttttttgtt
      601 ttgatcttaa gctgtagcta cgtagtacaa tctcttatat tactgataag gtctatttaa
      661 tgcgcaagat ttgtggtatg atgaatatga ttgagtattg atgctttatg ggattaccgg
      721 taaggttttg aaaaaggttt gtcactacta ggtgcttttt tagctataga aagacagtta
      781 tggattgatg tattgtaact aatgttggag gttaatggtg gttgagtttt gagatattta
      841 cactgataat cgggtggaaa ctggttgggt atcgggtaat ggagtgtggt tcttcgttta
      901 agcaaggaga ttatcgtttg gtggttcata tttgcttgtt aaatgttagg gatgagatgc
      961 tgagtcagca aggccatgaa gataaagctg actggccaga gccttgagat gtgacgtttg
     1021 gtggaagtcc tttttggtga gatggcacag catatagtaa tattagatcc tgaggaagaa
     1081 ttgggacttt cattaggctt gactggggta tgacctcact ttacaattca tttcgatgat
     1141 gggtttgatg atacgttttt agttcaggac tttgtagagt tggagaattt ggttctttat
     1201 gaggaagaag tgcaggtaat tcgttgagac agtcgtgaag atattttagg tatgattgat
     1261 gaaggtttgt tcattcctta tctgaagtat aaaataaatt tgtgctttga gatggttggc
     1321 caatataaag tccatcaatc ttagaagtaa taatttttga attttttctg aggtttgtgt
     1381 atatttgata gtgtactgat gtaagaagta aggagtacta tgatggcaat caatgagaga
     1441 gcttctgtaa aaatcaagtt aggccttgat actaagaata agcgatgaga tggttaccac
     1501 gatttatcga tggtcatgat gagtattgat ctgtgtgact atgtgacggt tgaccatata
     1561 gatgacaata agattgtctt tgcctcaaac tgtccgaaaa tacctattaa ctaagataat
     1621 gatgtttata agatagttca gctaatgaaa catcgatttc aagttaaaag aggagtttcc
     1681 gtttacttgg aaaagcggat tcccatgtgt gctggtatgg gaggaggttc tagcgatgct
     1741 gttactatca gagcattaaa tcagttatgg ctccttactc ttagccgaaa agatatgatg
     1801 gatattggta taccgattgg tagtgatgtg ccttattgct tgctttcagg tcgtgcccaa
     1861 gtcactggta aaggtgaagt ggtttgtcgc attttggggc tgttatcgtc ttgggtggtt
     1921 ttggttaaac cagattttgg tatctcgact tgaacatttt ttctagatat taattgtaaa
     1981 atcatttcaa gagtgtctac tactcatttg gttgcggcga ttgaagcggg taactataat
     2041 gatgggatat tgactgaaat gaacaatttg ctggaagata ttttcattgc caagcgaccc
     2101 tttattcaaa aaataaaaga gaaaacactg caagctggtg ctgctactac tttgatgaca
     2161 ggaagtggac cgactgtttt tgctttgtgc cagaccgaaa aacaaactat aatagtttaa
     2221 aaggactttg caaggaagtt tttaaggtga gaatgttgta atattttgtt gggattgctt
     2281 ctttggaagc ttcatgttat aattaactgg ttaatgaaag aggagattta tggggacttt
     2341 agaaaaaaaa cttgataact tagtaaatac tattttatta aaagcagaaa atcagcatga
     2401 gttattattt ggagcttgtc aaagtgacgt taagcttact aatacacaag aacatatttt
     2461 aatgttacta tctcagcaac gtctcactaa tacagatttg gctaaggcat taaatattag
     2521 tcaggcggca gtaactaagg ctatcaagag tttggtcaaa caagacatgc tagcaggaac
     2581 taaggatacg gttgatgcta gggtgactta ttttgaatta accgagttag ctaagcctat
     2641 tgcgtcagaa catacccatc atcatgatga aaccttaaat gtttacaacc gtttattaca
     2701 aaaattctcc gcgaaagaat tagagattgt agataagttt gtaacagttt ttgctgagga
     2761 attagaaggg taatccatga gatacatatc agtgaaaaat ctctcctttc aatatgaaag
     2821 tgagccagtt ttagaaggga tcacttatca tttagatagt ggagaatttg tcaccatgac
     2881 cggtgaaaat ggcgctgcaa agtcaacctt aataaaagca accttaggaa ttttacaacc
     2941 aaaggctgga cgagttacta ttgctaaaaa aaataaagac ggtaaacaat taaggattgc
     3001 ttacttgccg cagcaagtag ctagctttaa cgctggtttt ccatccaccg tttacgagtt
     3061 tgtcaaatca ggtcgctacc cacgtagtgg ttggtttaga catttgaaca aacacgatga
     3121 agagcatgtg caagcaagct tagaagcagt cggcatgtgg gaaaaccgtc ataagagaat
     3181 tggtagttta tcaggtggtc aaaaacaacg tgtggttatt gcccgtatgt ttgcttctga
     3241 ccctgatatt tttgtgctag acgagccaac aacgggaatg gatagcggta ctactgatac
     3301 cttttatgaa ctgatgcacc acagtgcaca tcaacacggg aaatccgttc tgatgattac
     3361 ccatgaccca gaagaagtga aggcttatgc tgatcggaac attcatttag tcagaaacca
     3421 aaaacttcct tggcgttgtt tcaacattca tgaagttgaa acagatgacg aaaaaggagg
     3481 tcatggtcat gcttgatatt ttatcctatg atttcatgca acgggcggta atggcggtag
     3541 ttgccattag tatttttgct ccgattttag gtattttcct tattttacgt cgtcaaagtt
     3601 tgatgagcga tacccttagt catgtttctt tggctggggt agcgcttggg gtagtccttg
     3661 gtatttcacc aaccatcact actattattg ttgtggtttt agctgctatt ttgttagaat
     3721 acctgcgtgt agtttacaaa cactacatgg agatttcaac ggcgattttg atgtcacttg
     3781 gcttggccct atctctgatt attatgagta agtcgcatag ttcatcaagc atgagtttag
     3841 aacaatacct ttttggatcg atcatcacga ttagtatgga acaagttgtc gccttgtttg
     3901 ctattgctgc gattatttta atcttgaccg ttctcttcat tagaccgatg tacattctga
     3961 cctttgatga agatactgct tttgtagatg gtttgcccgt tcgcttgatg tctgttctat
     4021 tcaatatcgt cactggggtt gctattgctt tgaccattcc agcagcagga gcacttttgg
     4081 tttctaccat tatggtcttg ccagcaagta tcgcaatgag attgggtaaa aactttaaaa
     4141 cagttatctt actgggaatt gtcatcggtt ttagcggtat gttatctggt attttcttat
     4201 cttatttctt tgaaacgcca gctagtgcca ctattaccat gattttcatt agtattttcc
     4261 tcttagttag tctaggtgga atgcttaaaa aacggttatt ttaataagaa aaatcctcac
     4321 atgtttaaca tgtgaggatt tatttataaa tgctttaaaa agggggtaag gtcccaattt
     4381 tcttgattaa tggtgatgct ctcccaaaag ctatcattag ttaaacgagg aatcaaatga
     4441 aggtgaaaat gagtcccctc atccatcaac tccttgtcat tgctgacgct agtgacgcct
     4501 gaaatgggaa ggtgctgctc tagcttagca actagagcgg cttgtaagtc gatgagatca
     4561 tagcgcactt cctttggtag ttgtgtcaga ctagtgtaat ggtctttgga aatcagtaaa
     4621 aggtgcccct tttggatagg atcaatatca aaaacaacct tgaaatattg actttgagtc
     4681 agtagctgat ttgctttgag ctgatggcaa aagatacatt ctgtcatagg tacatctcct
     4741 tatgaaaaaa cgagccagag ctcgtttaat cggttatcta gtaagtgaga acagcgtatt
     4801 ttttcttacc gcggcgaata acggttaatt gatcatcaat tttatcatca ttacttaatt
     4861 ggtaatctaa atcttgaacg cggtctccgt taatgtagat tgctccattt tgcacgtctt
     4921 cacgcgcttg gcgttttgat ggggaaatac ctgcggtgac aagcatgtca acaaggttaa
     4981 ggctgtcttc tgattgaacg tgataatttg gtacattgct caatccttgt tttaattcat
     5041 tagcagaaag atttttgatg tttccagcaa ataactgctc ggtaatgtta agagcttgtt
     5101 tataagcttc ttcgccatga accaaggtaa ccacttcgcg tgccaaggtt ttttgagcga
     5161 gacgctcgtg acgagcggca ttaaattgag tttcaatttc tgcaatctca tctagaaata
     5221 agaaggtgaa gattttcaag aaacgcactg catcatcatc catgacattt aaccagaatt
     5281 ggtacatctc gtaaggagac gtcttatcgg catcaagcca gacggcatta ccttctgatt
     5341 taccgaattt tttcccggtt gagtcagtga tgagtggtac agtcatgacg tgcccagttt
     5401 tatcagcctt tttgcggagt aattcagtac cagctgtcat attgccccac tggtcagagc
     5461 cgccaatttg taaggttaca ttatgcttgt cattgagttc gtagaagtcg tagccctgca
     5521 tgatttggta agcaaactca gtgtaagaaa tgcctgtttc aatgcgtttt ttaacagagt
     5581 ctttactcat catgtagtta acggtgaagt atttaccgac atcacggagg aagtcaataa
     5641 agctgatttg cgagaaccag tcatagttgt tgacaagctc tgctttatta tcaccatttt
     5701 caaaatcaag gaaagtagac aactgccctt taatcttgtc actccactcc aaaactgttt
     5761 ctttggtttg aaggctgcgc tctgcatctt taaaggaagg atcaccaatt aaacctgtgg
     5821 caccacccac aagagcgtaa ggtttgtgcc ctgctagttg caagcgacga gatgttaaga
     5881 tagccactag atggcccaga tgaaggctgt cagcggttgg atcataaccg gtataatagg
     5941 atacttgccc ttctgttaat gctttgacaa gggcttgttc atcagtcgtt tgaaagacca
     6001 agccacgagc tttgagttct tcaaaaatat tcataggctt tctccatttt cattatagtt
     6061 tcgaatcatt atatcaaaaa acgctactgc tatgcaagct ttatcaaagt ttggtataat
     6121 gataaatgag gtattttatg gtgaaatgga acacgaaaca aaagcgtata agtcatcaaa
     6181 gattaggtct cttggatttg gggccggtgc tattacgtac gttgagacta ctgtctaact
     6241 ttttttatat tgttatcttc ctttttggaa tgatgggatt tggtatggca tttgggtatt
     6301 tggctagtca gattgaatct gttaaggtac caagtaaaga aagtttagtc aaacaagttg
     6361 aatcattaac gatgatttcg caaatgaact attctgataa tagtttaatt tctactttag
     6421 atacggattt acttcgaaca ccagtagcta atgatgcgat ttcagagaat atcaaaaaag
     6481 ctattgtatc aacagaagac gaacattttc aagaacataa aggtgtcgtg ccaaaagctg
     6541 tttttcgggc aacattggct tctgtattgg gatttggaga agctagtgga ggttcgacct
     6601 taacacagca attggtcaaa caacaagttt tgggagacga tcccacattt aagcgcaagt
     6661 ctaaggagat cgtttacgct cttgccttag agcgttatat gtccaaagac aatatcttat
     6721 gtgattatct taatgtttca ccttttggtc gtaataacaa gggccaaaat attgctggtg
     6781 ttgaagaagc tgcgcgtggc atttttggcg tttctgccaa agatttaacg gtgccacagg
     6841 cagcattttt ggcggggctt ccgcagagtc ctattgttta ctctccttat ttgtcaacgg
     6901 gacaactgaa gtcagaaaag gacatggctt atggcatcaa gcgtcagcaa aatgttctct
     6961 ataacatgta ccgtacaggt gttctgtcta aaaaagaata cgaggactat aaggcttatc
     7021 cgattcaaaa ggattttatt caaccgggaa gcgcaatagt aaataatcac gattaccttt
     7081 attacacggt gttagcggat gctaaaaaag ccatgtatag ctatttgatt aagcgagata
     7141 aggtgtctag tcgtgacttg aaaaatgacg agactaaggc tgcttatgaa gagagagcct
     7201 taacagaatt gcaacagggt ggctatacca tcaccacaac cattaataag cctatttaca
     7261 atgcgatgca gacagcggca gctcagtttg gtggcctgtt agatgatggc actggtacag
     7321 ttcaaatggg aaatgtcttg acagacaatg cgactggtgc tgtgttaggt tttgttggtg
     7381 gtagagatta tgctctgaat caaaataatc atgctttcaa tacaattaga tcgccaggtt
     7441 ctagcattaa accgataatc gcttatggtc ctgctattga tcaaggttta atggggagtg
     7501 ctagcgtttt gtctaattac ccaacaactt actcgagtgg ccaaaaaatc atgcatgctg
     7561 atagtgaagg aacagccatg atgccacttc aagaggccct aaatacttct tggaacatcc
     7621 cagctttttg gacacagaaa ttactgcgtg aaaaaggtgt cgatgtcgaa aattatatga
     7681 caaaaatggg ttataagatt gcagactact cgattgaaag tttacctcta gggggcggta
     7741 ttgaagtctc ggttgctcaa caaaccaatg cttaccaaat gctttcaaac aatggcttat
     7801 atcaaaagca atatattgta gataagatca ctgctagcga tggtacagtc gtttacaaac
     7861 atgaaaataa gccaattcgt attttttctg cagcaacagc tacgatttta caagaattgt
     7921 tgagaggtcc gattacttca ggcgctacga ctactttcaa gaaccgtttg gcggctatta
     7981 atccgtggct tgctaatgct gattggattg gtaagaccgg aacaactgag aattatacgg
     8041 atgtttggct agtcctgtct actccaaaag ttaccttagg cggttgggca ggacatgatg
     8101 acaatacctc attagcgcca ttaacaggat ataacaataa ttctaattac cttgcctatt
     8161 tagctaatgc cattaatcag gccgattcca atgttattgg agtagggcaa cgtttcaact
     8221 tagatccagg agtcattaag gcgaatgtct tgaagtcaac aggtttacaa ccaggaactg
     8281 ttaatgtcaa tggacatact ttttctgttg gtggagaaat gaccaccagt ctatggtccc
     8341 aaaaaggacc gggggctatg acttaccgat ttgctattgg tggcacggat gccgattatc
     8401 aaaaagcttg ggggaacttt gggttcagaa aaaattaggt tgccatttat cgataaaaag
     8461 ggtataatag caataactat ttgtcgtcca atctagctga aatattgtcc agttaggaag
     8521 aacagcagtt aaatcacact gataaagtca gatttagctg ctctttttgt gtctattttt
     8581 agaaaagata gggtttgtaa cctatattta aatattctaa aaattaacat ttagggcaaa
     8641 tgataactta gttgcgattt gctgaaacag caaagcttaa atagaagaag gagctaaaaa
     8701 cttggcagga catgaagttc gatacggaaa acaccgtaca cgtcgtagct tttcaagaat
     8761 caaagaagtt cttgatttac caaatttgat tgaaattcaa actgactcat tccaagattt
     8821 cctcgattca ggtttgaaag aagtatttga agatgtactt cctatttcaa actttacgga
     8881 tactatggaa cttgaatttg ttggttacga atttaaagaa cctaaatata cccttgaaga
     8941 agctcgtatc cacgatgcaa gttattctgc accaatcttt gttaccttcc gtttggtcaa
     9001 taaagaaact ggtgagatta aaactcaaga agtcttcttt ggtgatttcc caatcatgac
     9061 ggaaatgggt acctttatta tcaatggtgg tgaacgtatc atcgtttctc agttggtgcg
     9121 ttctccaggt gtttatttca acgataaagt tgataaaaac ggtaaagttg gttacggatc
     9181 aacagtgatt cctaaccgtg gggcttggtt ggaattagag actgactcaa aagacattgc
     9241 ctacactcgt attgaccgta cgcgtaagat tccattcaca accttggttc gtgcccttgg
     9301 tttctcaggt gatgatgaaa ttgttgatat ctttggtgaa agcgaccttg ttcgtaatac
     9361 cattgaaaaa gacattcaca aaaatccaag tgattctcgt acagacgaag cccttaaaga
     9421 aatttacgag cgtcttcgtc caggtgagcc aaaaactgca gatagctctc gtagtctctt
     9481 gattgcgcgt ttctttgatg cacgtcgtta cgatttggct gcggttggtc gttacaaagt
     9541 gaacaaaaaa cttaatatca agactcgtct tttgaaccaa atcattgctg aaaaccttgt
     9601 ggatgctgaa actggcgaaa ttttggtaga agctggaact gagatgactc gtagcgtcat
     9661 cgaatccatc gaagaacacc ttgacggtga tttgaacaag tttgtttaca caccaaatga
     9721 ttacgctgtg gttactgaac ctgttgttct tcaaaagttc aaggttgtgt caccaattga
     9781 tcctgatcgt gtagtaacta ttgttggtaa tgccaatcca gatgataagg ttcgtgcatt
     9841 gacacctgct gatattttgg cagaaatgtc atacttcttg aaccttgctg aaggtcttgg
     9901 aaaagttgac gatattgacc acttaggtaa ccgtcgtatc cgtgccgttg gtgaattgct
     9961 tgccaaccaa ttccgcatcg gtcttgctcg tatggagcgt aacgttcgtg agcgtatgtc
    10021 tgttcaagac aacgatgtgt taacaccaca acaaatcatc aatatccgtc ctgtcacagc
    10081 agctgtcaaa gaattcttcg gttcgtctca gttgtcacag ttcatggacc aacacaaccc
    10141 attgtcagag ttgtctcaca aacgtcgttt atctgcctta ggacctggtg gtttgacacg
    10201 tgaccgtgct ggttatgagg ttcgtgacgt gcattacacg cactatggcc gtatgtgtcc
    10261 gattgaaaca cctgaaggac caaatattgg tttgattaac aacttgtctt catttggaca
    10321 tcttaataaa tatggtttca ttcaaacacc ttaccgtaag gttgaccgtg cgactggtag
    10381 ggtaactaac gagattgttt ggttgactgc cgacgaagaa gacgaataca cagttgcaca
    10441 ggccaattcg aaactaaacg aagatggcac ttttgctgaa gaaatcgtta tgggtcgtca
    10501 ccaaggtaat aaccaagaat tttctgcaag tgttgttgat ttcgttgacg tttcccctaa
    10561 acaggtagtt gctgttgcga cagcatgtat tcctttcttg gaaaatgatg actccaaccg
    10621 tgctctcatg ggtgccaaca tgcaacgtca ggctgtgcca ttgattgatc caaaagcacc
    10681 atatgttggt actggtatgg aatatcaagc tgcccatgac tcaggcgctg cggtgattgc
    10741 tcagcacaat ggtaaagttg tcttttctga tgctgaaaaa gtggaaatcc gtcgtcaaga
    10801 tggctcactt gatgtttacc acattaccaa attccgtcgt tcaaactcag gaacagccta
    10861 taaccaacgc acccttgtta aagtaggaga cattgttgaa aaaggtgatt tcatcgctga
    10921 tggaccttct atggaaaatg gtgaaatggc tcttggacaa aacccagtcg ttgcttacat
    10981 gacttgggaa ggttataact ttgaggacgc tgttatcatg agtgagcgcc ttgtgaaaga
    11041 agatgtctac acatctgttc acttggaaga attcgaatct gaaacgcgtg atacaaaact
    11101 tggccctgaa gaaatcactc gggaaatccc aaatgttggt gaagaagccc tcaaagacct
    11161 tgacgaaatg ggaattatcc gtatcggtgc tgaggttaaa gaaggcgaca tcttagtagg
    11221 taaagtcaca cctaaaggtg aaaaagacct ctctgctgaa gaacgtttac ttcacgccat
    11281 ctttggggat aaatcgcgtg aagtgcgtga tacctcactt cgtgtccctc acggtggtga
    11341 tggtatcgtt cgtgatgtga aaatctttac acgcgctaac ggcgatgaat tgcaatcagg
    11401 tgttaatatg cttgtgcgtg tttacatcgc tcaaaaacgt aaaatcaagg tcggagataa
    11461 aatggccggt cgtcacggaa acaagggtgt cgtttcacgt attgtaccag ttgaagacat
    11521 gccatacctt ccagacggaa caccagttga catcatgttg aaccctcttg gggtgccatc
    11581 acggatgaat attggtcagg ttatggaact tcaccttggt atggctgctc gtaatcttgg
    11641 cattcacatt gcaacacctg tctttgacgg ggcttcatca gaagaccttt gggacactgt
    11701 tcgtgaagct ggtatggata gcgatgctaa gacggtcctt tatgatggtc gcaccggtga
    11761 accatttgac aaccgtgtat ccgttggtgt catgtatatg atcaaacttc accacatggt
    11821 tgatgataaa cttcatgccc gttctgtagg accatactca cttgttaccc aacagccact
    11881 tggtggtaaa gctcaatttg gtggacaacg ttttggtgag atggaggttt gggcccttga
    11941 agcttatggt gcatcaaatg ttcttcaaga aatcttgacc tacaagtcag atgacgtcac
    12001 aggacgtttg aaagcctatg aagcgattac taaaggtaaa ccaattccaa aaccaggtgt
    12061 accagaatcc ttccgtgttc ttgtaaaaga attgcaatcg cttggtcttg atatgcgtgt
    12121 gcttgacgag gatgataatg aagtggaact tcgtgatctt gatgaaggtg aagacgatga
    12181 cattatgcat gttgacgatc tcgagaaggc acgtgaaaaa caagctcaag aaacccaaga
    12241 agtttctgaa acaactgacg aaaaataagc aatcaattct tattaaataa ttatttactg
    12301 gtctggggca aaggccccag gaactggtaa agtcatcaaa ggcagaaagg taaaactagt
    12361 ggttgacgta aatcgtttta aaagtatgca aatcacatta gcctcaccaa gtaaggtccg
    12421 ttcatggtct tatggtgaag ttaaaaaacc tgaaacaatc aactaccgta cattaaaacc
    12481 agaacgtgaa ggactctttg acgaagtcat ctttggtcca acaaaagact gggaatgtgc
    12541 gtgtggtaag tacaaacgta tccgttataa agggatcgtt tgtgaccgct gtggggttga
    12601 ggtaactcgt gctaaagtac gtcgtgaacg tatgggtcac attgagttaa aagctcctgt
    12661 atcacatatc tggtatttca aagggattcc atctcgtatg ggattgactc ttgatatgag
    12721 tcctcgtgcc cttgaagaag tgatctattt tgcggcttat gtggtcattg atccaaaaga
    12781 tacaccgctt gaaccaaaat cattattaac agaacgtgaa taccgtgaaa aactacaaga
    12841 gtatggtcat gggtcatttg tcgctaagat gggtgctgaa gccattcaag atctcttgaa
    12901 acgtgtggac ttggcagctg aaatcgctga attaaaagaa gagttgaaat ctgcttctgg
    12961 tcaaaaacgg attaaagcag ttcgtcgttt agacgtcctt gatgccttta acaaatctgg
    13021 aaataaacca gaatggatgg ttcttaacat cctgccggtt attccaccag atcttcgtcc
    13081 gatggttcaa ttggatggtg gccgttttgc ggcatcagac ttgaatgact tgtaccgtcg
    13141 tgtgattaac cgtaataacc gtttggcacg tttgttagaa cttaatgccc ctggcatcat
    13201 tgttcaaaat gaaaaacgaa tgcttcaaga agccgttgat gctttgattg ataatggtcg
    13261 tcgtggtcgt ccaatcactg gaccaggaag tcgtccattg aaatcattga gccacatgct
    13321 taaaggtaaa caagggcgtt tccgtcaaaa cttgcttggt aaacgtgtag acttctctgg
    13381 acgttccgtt atcgctgttg gtccaaccct taaaatgtat caatgtggtg tcccacgtga
    13441 aatggctatc gagcttttta aaccatttgt aatgcgcgaa attgttgcca aagaatatgc
    13501 tggtaacgtt aaagccgcta aacgtatggt tgaacgtggc gacgaacgca tctgggatat
    13561 cttagaagaa gttatcaaag aacacccagt cctacttaac cgcgcaccga cccttcacag
    13621 acttggtatt caggcttttg aaccggttct tattgacggt aaggcacttc gtcttcaccc
    13681 acttgtgtgt gaggcctaca atgccgactt cgatggagac caaatggcca tccacgtgcc
    13741 actttcagaa gaagcacaag ctgaagctcg tcttttgatg cttgctgcag agcacatctt
    13801 gaaccctaaa gatggtaaac cagttgttac tccatctcag gatatggttc ttggtaacta
    13861 ctatcttacg atggaagatg ctggtcgtga aggcgaaggt atgattttca aggataaaga
    13921 cgaagctgtg atggcatatc gtaatggtta tgctcatctt catagtcgtg tgggtatcgc
    13981 tgttgacagc atgccaaaca aaccttggaa agacagtcaa agacataaaa tcatggtgac
    14041 aactgttggt aagattctct ttaacgatat catgccagag gaccttcctt acctccaaga
    14101 gccaaacaat gccaacttga cagaaggaac acctgataaa tacttccttg aacctggtca
    14161 agacatccaa gaagtgattg atcgcttaga catcaatgtg ccatttaaga agaaaaacct
    14221 cggtaacatc attgcggaaa ccttcaaacg tttccgtaca acagaaacat cagccttcct
    14281 tgaccgcttg aaagaccttg gttactacca ttcaaccctt gccggtttga cagtgggtat
    14341 cgctgacatc ccagttatcg ataacaaagc agaaatcatt gatgctgctc accatcgtgt
    14401 tgaagaaatt aacaaagcct tccgtcgtgg tttgatgaca gatgatgacc gttatgttgc
    14461 cgttacaaca acatggcgtg aagctaaaga agcccttgaa aaacgtctaa ttgaaacaca
    14521 agatcctaag aacccaatcg ttatgatgat ggactcagga gctcgtggta acatctctaa
    14581 cttctcacag cttgctggta tgcgtggttt gatggctgct cctaacggac gcatcatgga
    14641 acttcctatc ttgtcaaact tccgtgaagg tttgagcgtt ttggaaatgt tcttctcaac
    14701 ccacggtgca cgtaaaggga tgaccgatac ggcccttaaa acagccgact caggttatct
    14761 tactcgtcgt ttggttgacg ttgcccaaga tgttatcatt cgtgaggacg attgtggcac
    14821 tgatcgtggt cttcttatcc gtgctattac agatggtaaa gaagttaccg aaacgcttga
    14881 agagcgtctt caaggtcgtt acacacgtaa atcagtcaaa caccctgaaa ctggtgaagt
    14941 cttgattggt gctgaccaat taatcactga agacatggct cgtaagattg ttgatgcagg
    15001 tgttgaagaa gtgaccattc gttctgtctt tacctgtgcg actcgtcatg gtgtctgccg
    15061 tcactgttat ggtatcaact tggcaactgg tgatgctgtt gaagtgggtg aagcagttgg
    15121 tactattgct gcccaatcta tcggtgagcc tggtactcag cttaccatgc gtaccttcca
    15181 cacgggtggt gtagcctcaa ataccgatat cacacagggt cttcctcgta ttcaagagat
    15241 ctttgaagca cgtaatccta aaggggaagc ggtcattact gaagtgaaag ggaatgtcgt
    15301 cgagattgaa gaagatgcgt caactcgtac caagaaagtc tacgttcaag gaaaaactgg
    15361 catgggcgaa tatgtcgtac catttacagc acgtatgaaa gttgaagttg gcgacgaagt
    15421 taatcgtgga gctgccctta cagaagggtc aattcaaccg aaacgtctcc ttgaagtgcg
    15481 tgataccttg tcagttgaaa cgtaccttct tgcagaagta caaaaagttt accgtagcca
    15541 aggggtagaa atcggagaca aacacgttga ggtaatggtt cgccaaatgc ttcgtaaagt
    15601 tcgtgtcatg gatccaggtg atacagacct ccttccaggt acacttatgg atatttctga
    15661 tttcacagat gctaacaaag atattgttat ctctggtggt attcctgcga catctcgtcc
    15721 tgttcttatg ggtattacta aggcttccct tgaaaccaat tccttcttat cagctgcatc
    15781 cttccaagaa acaactcgtg ttcttacaga tgctgctatc cgtggtaaga aagaccactt
    15841 acttggtctt aaagaaaatg ttatcattgg taaaatcatt ccagctggta ctggtatggc
    15901 gcgctaccgt aacattgaac cacaagcgat gaatgagatt gaagtgattg atcatacaga
    15961 agtctcagca gaagccgttt ttacagcaga agcagaataa tctcatgaaa gagtccttag
    16021 gggctctttt ttgtctgagt ttgttggcag taaaaagcat tgacaatcag tcttagtccc
    16081 aatgagcaga tgtttttcaa aatacttatg atttagctat aataatgcat aagaggaata
    16141 ggtaatgtgt tatgtatcaa gtaattaaaa tgtatggtga ttgggagcct tggtggttca
    16201 tcgatggatg gcaagacgat attatagatg agcaacaatt tagtgactgg caagaagcgc
    16261 ttgattattt taatcaagaa tggcagcgca tgaaagctat ttttcctagt tatcatagtc
    16321 aaaagaattt gttagctact ttttgggaaa aagaagataa aagatggtgc gaggactgtg
    16381 acgaagattt acagcagttt cattctcttt tactccttaa aaataaagat attgtaccaa
    16441 gcaataatta tatccctgaa tttgaacaac gaaatgattc accacaggta gcttatcttt
    16501 gcaagttaaa cctttaagtg tttgttaaaa acaaaataag ttaaaagaaa atatcacttc
    16561 ttctgatatt ttcttttttt gtttacgaat aaatgagtaa ggaggaatta tggtacaagc
    16621 attagcaaaa gctattctag caaaagctga acaggttcag gcacaagata tttatatttt
    16681 gccaagagca gatcaatatg atcttttttt acgaatagga gatgaaagga gattagttga
    16741 tgtttatcag agcgatcgga tggctcctct tattagtcac tttaaattcg ttgcaggaat
    16801 gatagttggt gaaaagagac gttgtcaggt gggttcatgt gattataagc ttagtaaaga
    16861 taagcagtta tctttgcgct tatctagcgt gggtgattat cgcgggcaag aaagcttagt
    16921 gattcgtctg cttcatcatc aaaataaaag tgtacattat tggtttgatg gattgacaaa
    16981 agtagccaat caggttggcg gtagagggtt gtatttattt gcaggaccag ttgggtctgg
    17041 gaagacaacc ttgatgtacc agctgatttc gagttatcat caagaagcac aggttattag
    17101 tatagaagat cctgtagaaa ttaaaaatca ccagatttta caattacaag tgaatgatga
    17161 tattggtatg acttatgaca atttgatcaa actgtcttta cgccatcgac cagatatttt
    17221 agttattggt gagattcgag atagtcaaac agcaagagcc gttattaggg ctagtctaac
    17281 aggtgccatg gttttttcaa cggttcacgc taaaagtatc tcgggtgttt atgtaagatt
    17341 gttagaactt ggtgtaacga aagcagaact gtctaattgc ttagcattaa ttgcttacca
    17401 aaggttactt aatggaggag cattgattga ctctactcaa aacgaatttg aatattattc
    17461 ctcatcgaac tggaatcaac aaattgatca gcttcttgag gcaggacatc tcaatcccaa
    17521 gcaagctaag cttgaaaaaa ttatctagta aacatcagca taaattcatt caattactag
    17581 ctaatctttt atctacagga tttagcttcg cagaggttat tgctttttta aaaagaagcc
    17641 agttgcttca gctagattat gttcttaaaa tggaagagtc tttattaaaa ggacaagact
    17701 tagcagatat gttgtcgggt ttaggttttt cagatgctat tcttactcaa attagtttag
    17761 ctgatagaca cggtaatatt gaaacaacat tagtagcgat tcaacattac ttgaaccaaa
    17821 tggcaaggat cagacgaaaa actgttgaag ttatcaccta tcctcttatt cttttacttt
    17881 ttctttttgt gatgatgcta gggttgagac gctatttagt ccctcaatta gaaactcaaa
    17941 atcagataac ttactttctt aaccattttc ctgccttctt tataggtttt tgctcaggtc
    18001 ttattttgtt gttcgggatg gtatggctac gatggcgatc tcaaagccgc ttgaaactct
    18061 atagtcggct aagccgttat ccttttttag gtaaactgtt aaaacaatac ttaactagtt
    18121 attatgctag agaatggggt acgttaatag gtcaaggtct tgatttgatg actattttag
    18181 acattatggc cattgagaaa tcctcactaa tgaaagaatt agctgaggac atcagaatga
    18241 gtctgcttga agggcaagcc tttcacatta aagtagctac ctaccctttt tttaaaaaag
    18301 aattaagttt aatgattgaa tacggtgaaa tcaaatcgaa actgggggct gagttggaaa
    18361 tctatgccca ggaaagctgg gaacaatttt ttagtcagct ttatcaagtg acacaactta
    18421 ttcaaccagc tattttttta gtagttgctg tgacaatcgt catgatttat gcggcaatcc
    18481 tattaccaat ttaccaaaat atgggaggta ttttttaaga tgattaatca atggaacaac
    18541 ttacgacaca agaagctaaa aggatttact cttctagaaa tgttattggt gattcttgtc
    18601 atcagtgttt tgatgctatt atttgtgcct aatttaagca agcaaaaaga cagggttaca
    18661 gaaacaggta atgccgctgt tgttaaatta gtggagaatc aagcagaact atatgaatta
    18721 tctcaaggct caaaaccaag tttgagccag ttaaaggcag atggtagtat cactgagaaa
    18781 caagaaaaag cttatcaaga ctattatgac aaacataaaa atgaaaaagc ccgtcttagc
    18841 aattaaagct tttactttgc ttgaaacttt actaagtttg tcagtgatga gtttcattat
    18901 attgggattg tctgtaccag tgacaaaaag ctatcaaaaa gtagaagagc atctgttttt
    18961 tagccatttt gaacatttgt atcgacatca gcaaaagtta gccatattac agcaaaaaca
    19021 gcgcgtcttg gatatttctt caacaaaaat tgttacagaa ggtaatagcc taacagttcc
    19081 taaatcaata acagtgaatc atccttatcg gttagttatt gatcagatgg gaggcaacca
    19141 ttccttagct aaaattattt ttgacatgac tgatcgacgc tttaaatacc aattttatct
    19201 agggagtggg aattatcaaa aaacaagtca aagcttacat agcccttgaa agtatgatcg
    19261 caacaggcat cctctttagt attgtcattc tcgtcttaag cagtttacag cagagtcagg
    19321 ctgctctaac gtactatcga aagcagcaag aaaagcttaa tctagccttg atggcagtac
    19381 aaactagaac taaagagata acattgaatg gttgtcatat tactatttta cgtacggata
    19441 gatacattag tattcacgat gacgaaggag aagtgatgaa gattgagtaa acaattaagt
    19501 aacataaaag cttttaccct tctagaggcg ttaatagcct tactcgtgat atcagggtct
    19561 ttattggttt atcaaggttt gacccaaacc ctccttaaac gtagccatta tctagcccgt
    19621 catgatcaag acaattggct cttattttct catcaattgc gagaggagtt aagtggagca
    19681 agattttaca aagtagctga taataaacta tacgttgaaa aaggaaagaa agtactagct
    19741 tttggccaat ttaaaagtca tgattttcga aaatcagcta gtaatggaaa agggtatcaa
    19801 cccatgttat ttggaatatc acgtagtcat attcacatag agcagtcaca gatttgcatt
    19861 actttaaagt ggaaaagtgg gttagaaagg actttttatt atgcctttca agactaatct
    19921 caaagcaggc atccttttgt atgctatgtt tatggctagt atttttttgc tggttttaca
    19981 ggtttacctt tctcaagtga cagctctaca caaggaatac caagctcaaa ctgactatgt
    20041 taaagcccga ctgatagcag aaatagttta tcaagatcat agatataaag cttctaatcc
    20101 tgtctttttt aaaggtggtc aagtcatttg tcgagagcga aaagagagat ggatgttaat
    20161 tgttaagtta gatcagcaac gacagtatca gtttgaatac cttaaaccca aacaatatag
    20221 tcaataaata gtattgttgt cataaatcca taaaatgatc caatatatag taattaaagg
    20281 tgacacttct tttcatttca agtgtaattt ggtatgataa ggttatgact tttgaaaaaa
    20341 ttgaagaagc ttatcagctg ctcttagaga actgtcaact aattgaaaat gatctaaaaa
    20401 cacatatata cgatgccatt gttgaacaaa actcctttta tttaggggct gagggagcta
    20461 gtcctcaagt tgctcaaaat agtgataaac tgaaagcctt gtgcctgaca aaagaagaat
    20521 ggcgtaaggc ctaccagttt ctttttatta aggcagctca gacggagcaa ctccaagcca
    20581 accatcagtt cacaccagat gctattggct tcattctgct gtatcttttg gaacaattga
    20641 gtgataaaga tagcttagag gtacttgaga ttggaagtgg aacagggaac ctagcccaaa
    20701 cccttctcaa caacacgagc aagagccttg attatgtagg gattgaactt gatgatctct
    20761 tgattgatct gtcagccagt attgctgaga taatggattc ttcagctcat tttattcaag
    20821 aagatgcggt aaggcctcaa ttactaaaag aaagtgacat tgtcatcagt gacttaccag
    20881 ttggttatta tcctaacgat gatattgcca aacggtacaa ggtggctagt tcagataagc
    20941 atacctatgc ccatcattta ttaatggaac agtctttaaa atacttgaaa aaagacggtt
    21001 ttgcgatttt tctggcacca gtcaatttat tgacgagccc tcagagccag ttattgaaac
    21061 agtggttaaa agattatgct caggtggtga ccttgattac gctaccagat tctatttttg
    21121 gtcatccctc aaatgccaag tccattattg tcttacaaaa acaaacagac cacccaatgg
    21181 aaacctttgt ctatccaatt cgggatttga agcttgcaga gaatattcat gattttatgg
    21241 aaaatttcaa aaaatggaaa ctgagtaatg tcaattaatt gattttttgt tataataaaa
    21301 aagcatcaca gaaaacgatt tcaagagagg ttaaaaatgt caaagacaat tgcaattaat
    21361 gcaggtagtt ctagtttaaa atggcaactt tatcagatgc cagaagaaga agtgttagca
    21421 caaggaatta ttgagcgtat cggtctcaag gattctattt caacagttaa gtacgatggc
    21481 aaaaaagaag agcaaattct tgatattcac gatcatacag aagctgttaa aatcttattg
    21541 aacgatttga ttcactttgg aatcattgca gcttatgatg aaattacagg agttggacac
    21601 cgtgttgttg ctggtggtga acttttcaaa gaatccgttg tggttaatga caaagttctt
    21661 gaacagattg aagaattatc cgttttagct ccacttcaca atcctggagc ggcggcaggg
    21721 attcgtgcct tccgtgatat tttgccagat attaccagtg tttgtgtgtt tgacacgtca
    21781 tttcacacta gcatggcaaa gcatacttac ctttacccaa ttcctcaaaa atattatacg
    21841 gactacaagg ttcgtaaata tggggcgcat gggacaagtc ataaatatgt agcgcaagaa
    21901 gctgctaaaa tgcttggtcg tccattagaa gaattaaaac taattactgc tcatattgga
    21961 aatggtgtct caattacagc taattaccac ggtaaatcag ttgatacctc gatgggcttt
    22021 acaccacttg ctggtcctat gatgggaaca cgttctggtg acattgaccc agctatcatt
    22081 ccttatttaa ttgagcaaga cccagaatta aaagacgctg ctgacgttgt gaatatgtta
    22141 aacaaaaaat caggtcttag tggggtgtca ggcatttcta gtgatatgcg tgatatcgag
    22201 gctggtttac aagaagataa tccagatgct gttttagctt acaatatctt tattgatcgc
    22261 attaaaaaat gcattggtca atatttcgct gttttgaatg gagcagatgc tttggtcttt
    22321 acagctggta tgggtgaaaa tgcaccattg atgcgtcaag atgttattgg tggcttaaca
    22381 tggttcggaa tggacattga tcctgagaaa aatgtttttg gataccgtgg tgaaatttca
    22441 actcctgagt caaaagtgaa ggttcttgtt atctcaacag acgaggaatt gtgcatcgcg
    22501 cgtgacgttg agcgtttgaa aaatacaaaa taaaagagac taagttttca tagattaacc
    22561 aagtaaggca acactttact tggtttttta tgaggtttat ctgttagtta tggtgcctag
    22621 gtattagttc tgatcagtct ccttgctttt tgtaatcttg tgctaggcga tttggatgtc
    22681 agccttattt agaagttttt aattgcttca acgcttttct tgtatgtgtt tatagagccc
    22741 aatgatctaa aagactattt gataacggtt tatgatgagc gtaatcgtgc gattcgaaaa
    22801 cagacacaag tttactgctt ttacctgatg attgttctat ttgttgcctt attctttttc
    22861 ttcccttgct taaccattcg agaccttgtt tcttataagc tagctttgat ttttgcttac
    22921 ctttacttga ttatcagaag tctggtggct aagaaaatgg aggcactagg gaaaataaca
    22981 actaatagtc aaaaacctcc gtttgatacg agtcaggttc tttgttagat gacttgtcta
    23041 acggttgagt ttcctgtctg gtcaacggag gtgtttgtat tgttgtctga tttgtattgg
    23101 ggcacttatt ttacaagctt ttagccttat cgatggtttt gtcaatggca gagctgacag
    23161 tagttgtgag gccaaggcgt tctaactcca ttagaccagc aatagttgtg ccaccggggc
    23221 tacaaatagc gtcaatgaaa tcgtgcggac tttgagaact ggtcttgaga ttgctggcgc
    23281 tagccaagac tgtttgagta acaatctcca gcgcctttgc tttaggcatg ccattcttga
    23341 cgccagcctt agccaaagcc tcaataaaga gataaatata ggcaggactt gaccctgcta
    23401 aagcggtaaa ggtgtcaaaa tccttttcac taatatcaaa tgtgctacca aagctatctg
    23461 ttaagtttcg aacacgtgct tgtaattcct gggacaccaa agcatttccc gttaaagcgg
    23521 tactgctttg gagaatttgt gcattcatat ttggcatgat acgtagcagc ggaaggtctt
    23581 gtcctacgaa tgttgctagt cgttgaaggg aaatgcctgc tgccatagat ataataggct
    23641 gtttgaagtg aagcggtttg agtaccgttt caaatagttg aggcttgatg cctaaaataa
    23701 caagatcaac ctggtcaata aggtcttggt gggacatagc ataaggcagt gttaactgct
    23761 ctgcaatttc cttggaccgt tctaaagatg atcctgaaat gataagttca tggggtgttt
    23821 gtttaaggcc tttgatgata gcgctagcca ttttgccaac accaataatg ccaattttca
    23881 ttctgttgct ccttgtggga tattacggtt aatcaggcta acgctagttt agtaacgttt
    23941 aatcaagtca acggttgagc gatccagttt tttgataatg gcttgtaaga aggcttgggc
    24001 ttctacgaaa tcatccatag cgtagagggt ttgatgagag tgaatgtagc gtgcacagac
    24061 tccgatggtt gttgatggga caccaccatt ttgaaggtgt gcagcacctg catctgtgcc
    24121 tcccttgcca caatagtatt ggaaattgac accagcgtcc tcagcagtag tcagtaagaa
    24181 gtcgcgcata tctttgagca tgacatggcc tgggtcgtaa aagcgcaata aggtaccatc
    24241 tccgattgtt ccaggattgc cataaatatc accagcaggc gaacaatcta ctgcgaaaaa
    24301 gagttcaggg tcgaacttgg tggttgagac gtgggctccg cgcagaccaa cttcttcttg
    24361 aacgttagca cctgcaatta gggtgttgtt aaggtcttgt ccttttaacg cttcaagcat
    24421 ttctgttatc atgagaacgc catagcgatt gtcccaagct tttgaaataa tatttttttg
    24481 attggctgtt aggatcgttt cagattgagg gacaataata tcacccggtg taatgccaaa
    24541 gctttcagct tctgccttat ccgtaaagcc accatcaaac acaatatttt cgatatttgg
    24601 tagactagca gagccatttg ccccacgtaa aaaatgggga ggtaccgatc ctgaaataag
    24661 gggaataact tggccagtgc gtgtgtaaag ggtaaaccgt tgtgaactga caacaagtgg
    24721 gttccaacca ccgataccaa ccacgcgtag cgttccgtca actttgatat cactgaccat
    24781 aaaaccgact tcgtccatgt gcgcagctac taaaatacgg ggggcttttt cagctttact
    24841 atctctgata ccaaaaatgc caccaagccc gtctgtttca acacggtcaa ccagcggggt
    24901 tattttggtg cgtaggtagt cacgaacgct atgttcatag cccgcaatgc catccagttc
    24961 ggtaacttct ttgatttttg agaataagtc tgtcattttt catctccatt taaaaggttt
    25021 cagaccagtt caacgcggtc atctccttat attctaccac tttttaagta agaatgggtt
    25081 tcacagaaaa gaaaaatccc agagactaga gatctctgga agaagtgttt gtcttttggc
    25141 gataacctgt ttttttaggc agcaaggaaa tcattaaaat gataaagcac aagctggtta
    25201 ataagaatag tagggcgctt aggatgcgaa aagactcttc actagcgaaa agtaaggata
    25261 atggagtcat aaggagaacc ttccatttta ggttaatcag tattttttat agcgtggtta
    25321 aattaataat aaaccacacc taatcaatgt acttttagtt tagttgtttt tactattttt
    25381 gtcaatacat aacagtagaa atctttagtt ttcttatttc agatcgtcca atttgatctt
    25441 tttatgataa aatgagggat atgagtaaaa agaaaatagg tatgatttca ggtatctttg
    25501 gatttagttt agctattggc ctgggaatag ttatcaaaga ttattgccaa gacaggcagc
    25561 gccgacaaat gacaagggat ttacgcacct ttttttcacc tttaggacaa atcgaagttt
    25621 tatatatcaa tccttgtcag gtcaaacaag attatatttc aggtggggtt gttatgtcaa
    25681 acggcaaaca gtaccaattc acctatcaca gtcgacaaat tagttttgag gaggacaagg
    25741 gatgatcaga ccgacttctt atgagtcatt agcggctctt attgaaaaag aagataagct
    25801 ggtgttattt tttacggcag actggtgtcc agactgtcaa tttatttatc ctattatgcc
    25861 agaaattgaa gcagaactta cagatatgac ttttatttgt gttaatcgtg atcagtttat
    25921 agaggtagca caaaagtgga atatttttgg tattcctagt tttgtcgtga ttgaaaaagg
    25981 tcaggaagta gggcgtctgg ttaacaaaat gagaaagaca aaaactgaga ttatgcattt
    26041 tttagctgct tatcaataag aaaagaggaa gaataaatga tttttgcata caataaagaa
    26101 caagttggcg atgtcttgat ggttatctta caagacacca aagatatcaa acgtcaagta
    26161 gaacgaaaag gcaaagtagc ccgtgttttt gcagaagaaa gcggcaaaac ccttgcttgg
    26221 aatatctttg aagtgtcaag cttgattact attgaaggca atggacagat ttttttgaca
    26281 gacgaggacc ttgcaagatt aaatgcagag cttgccaagg aaggattttc agaaaggctt
    26341 gaaccgactg tgggacctgt ttttgtggtt ggtcaaattg ttgagatggt ggctcatcca
    26401 gatagcgacc atcttaatat ttgtcaagtg gctattggtg aagatcaaac ggttcaaatc
    26461 gtagcgggcg caccaaatgc tgcgcttggt ttaaaaacga ttgttgcctt accaggtgct
    26521 ataatgccaa atggtagtct gattttccca ggaaaattgc gtggtgaaga aagttatggt
    26581 atgatgtgct ctcctcgtga gttggtcttg ccaaatgcac cgcaaaaacg tggcattatt
    26641 gaacttgatg agtcagctgt ggtaggagaa gcttttgacc cagccaaaca ttggaaaggt
    26701 tagaaaaacc agctgaaata agactgtatt taactaaatg accagaaaaa aaggattagg
    26761 agcccttaat ccttttttct tatgttttca aaaacgttta aaatgaaact tattttacca
    26821 caatttttac gaataataag ataaggaggt cagtatgtat aataaagtga tagcaatcgg
    26881 tcgtttggta gctaaaccag aattggtaaa aaccgctacg gataagcatg tagcacgtct
    26941 ctctttagct gttaatcgaa gatttaaaaa tgcttcggga gagcgagaag cggattttat
    27001 ttcaattgtt gtttggggaa agttagcaga aactctggtt tcttatgcta gcaaaggtag
    27061 tttgatgtct attgatggcg aacttaggac ccgcaagtat gataaagatg ggcaagtgca
    27121 ttatgtgaca gaagttctct gccaatcatt tcaactgctt gaaagtcgtg ctcagcgcgc
    27181 tatgagagaa aataatgtta ctaatgatct agttgattta gtcttagaag aagatactct
    27241 tcccttttga tagaggctaa tgcttctcat cagggttcag ctgattactg aaatatatga
    27301 tgaggtgata tccgagcaac aaaagtgtga ctagatactt ctgataaaaa taaaaaaaac
    27361 accttacgag tatgaacaaa gtttcaagtg ctagatgttg gaactttgtt catcatagga
    27421 atagtagggt gttttttagt gtattacttc tttttgttgc tagtgttaag gtttacttgc
    27481 ttacaagcgg ttatcaggcc actagaactc ttataataaa tggagctctt tgagtttggt
    27541 gtcaatcatt tttaagacag tcgccaaatc ttgtgggttt tgaacgaaat caagggaatt
    27601 gccatcaatt ttcatttttg gagagacctt gtagtcttcg taccaggttg ggtattcacc
    27661 gtggacttga tggtaatatt gctcaagact aggattgccg tcgacctgct caaaacttcg
    27721 gccacgtttt tcaatgcgct caagcatttt gtcaaaagac acatcaatgt agatgagaag
    27781 atcagggcgt tttttaggca tgccttctag ttcttctaac atgttggcga gaagttcctg
    27841 atagatgtca agttctgtct tagtgacatt gccatttttg tagttaagtt ttaagaagag
    27901 ttcgtcttca aaaatagagc ggtcaagaat gttgttatcg gcttgataag cttctttgat
    27961 ggacttaaac cgtttattca aaaaataaat ttgaagtaaa aatgcgtact ttttagggtc
    28021 ttggtagtag aggtctagca cggggttatt atcaacagct tcataaaaaa cgtcagttcc
    28081 taagtgctct ccaagtgcag cagcaagcga gctcttgccg gccccaattg ttccagctag
    28141 tacaatcaac atgcgtctcc ttctaatatc attagcgtac tgttttgaag atggcttcaa
    28201 cctcagctag agtctcagcg cgtgaaacag caccacgaac tttggcagca cctgctgttc
    28261 cgcgaagata gtgtggcgct aagccccgaa attcccgaac agcaatggtt tcacctttga
    28321 ggttaatcag gcgtttgagg tggtctttgg cgatgtctaa ttttttggca aagggaagat
    28381 ctggcaattc ttgaccagtt tcaaagaaat gattgatttg ggtaaaaaga taaggattgt
    28441 tcatggctgc gcggccaatc atcacggcat ctacaccgat ttcttcaatc ataaatttgg
    28501 cgtcttgcac cgaacgcacg tccccatttc caataaaggg aatcttagtg atagccttag
    28561 aaactcgggc taatgtctca tggtcgcagg ttcctgtata catttgttca cgggtacgac
    28621 cgtgcatggc aagagcagat acacctgccg actcagcggc aagggcattc tcaacggcaa
    28681 gtgaactatc cgcccaacct gtacgcattt tgacagtcag tggaatatct aaaacagacg
    28741 tgacttcctt gacaatgtgg taaatcttat cagggtcacg gagccacttg gcaccagcct
    28801 cgtttttgac aactttatta acagggcagc ccatattaat atcaacaata tcggccttgg
    28861 tattggtttg aatgaaatca gctgcacgtt tcaacccttc tgcgtcacca ccaaataatt
    28921 gaatggacat agggtgctca ttctcgtcaa tatggagcat gtgaagggtc ttttcgttgt
    28981 tgtagagcag ccctttttcg gaaatcattt ccataaccac caaaccagca ccaaattctt
    29041 tggcgatggt acgaaaggca gaattggtca cgccagccat aggggcaagt acagtgcggt
    29101 gggggatttc cacatcacca atcctaaagg aagaattaag ctttgtcatt gatgagggcc
    29161 tccaaatcat tttcattaaa ttggtacttg gtaccacaaa attggcaaac aatctcagca
    29221 cccttatctt cgtcaatcat ggcctgtaaa tctgcttttg gcaaagtcat tagggctgct
    29281 tcaaaacgtt ctctagaaca gtcacactga aaactcagag gttcttcaga aagacgcttg
    29341 taaggttcat ctccatagat ggcctccaaa agggcatcca catgattttt ggaagctagt
    29401 aggtgagaaa tggcaggcat ttcttgcagg cgtttttcat agcgagcaat ctcttcttca
    29461 gaagctcctg gtagcacctg caccataaag ccaccagcaa ccttgacctt atcattttca
    29521 tctaaaagca cattaagtcc gattgcagat ggtgtttgct ctgattcggt taagtagtag
    29581 gcaaaatcct caccaatttc cccagtaata agaggcgttg ttgaggtgta agggttacct
    29641 gtgccataat caataatcgt cacaaaatga ccattcccca taaaaggccc aacaaggact
    29701 tcacctgttg cggtcttttt aatatcgaca ccagtatttt gaatgtaacc tttcacatgt
    29761 cctttggtat ctgccacaga gataatgtgt ccaaaagaag aatcaccaat cactttgacg
    29821 gtgattttac tatcgccttt ttgattagcc gccaaaatct ggttggcgat caaggtacgg
    29881 ccaagcgcaa cagtagacga tgacagcgtg ttgtgttttt cttgagctaa tgcaaccgtc
    29941 tcagtgctat caaggacata agccctaaaa gcccctgatt gtgctattga tttaattatt
    30001 ttatccataa cttagattat agcataaagt gtaaaacttg tattgacagg attgcttgaa
    30061 aaatcaatac agatagacag taaaaaatcc cagagaatga ctcgggatta agttaaggtg
    30121 tggtctaaag tcgcgcaatg tggtgacatg gtgcttatgt taattgcttg gttaaatcag
    30181 cttggaattt ttcctcttta acttgataca tcaattcttg gatagacaga atcgattcat
    30241 caaaagatat ttcagcaaca gcaattcctt ttgtaagttc atggtgaaca aaaggaatca
    30301 gttgactgtg agtgatgacc aaatctggct ttaaatcagg aatttgataa acattatctt
    30361 gcaatagata ataggaatga aaatcaatgc ttttatccga gaaatacctt ggaaaagaat
    30421 ccgttaggag atgagcattg ataaaattac tggctacgaa aacaacaact aaaggaggtt
    30481 ggatatttct tagactttgc tcgatatagt gacaaaaaag atgaaaatgc ttatggttca
    30541 agtcacgctt accaggaagt ttggccatcc actcttcgac aattaacttt aaggacgtat
    30601 agagtttttg gtttccttta tagtacggag aaacgaataa gttggtctca ggaataaaat
    30661 gttgcagatt aaacaagaat gattttgaaa aaaacataag agcttttact aaactagcct
    30721 tttgctcttt taggttaggt aaaagagtga tgataggatt taaaagcagg cgaaaagtat
    30781 cattttcttc aaaaagttga caacattgtc tgatatgctc aggtgtccat tgtaagctcg
    30841 caaaagaatt attagcggtg atataaatta aatagaggta gtccaaatct cctgctgaaa
    30901 agtttagttg gcagtaagtt tcgataatat catggctact ttttttcaaa gaatcataga
    30961 caaaaagttt ttttaattgt tgaaaaattc tggtttgggg aatagttacc gaaaattgat
    31021 gccgcttcca cgataaagcc aataaaatgt catagaaaga aaacgattcc gataaccaag
    31081 gagaggtttt aaggtgggtg gaactatggg ataaaaagct atgaatagtg tttttgtctt
    31141 gctgcgtcaa gtcataaact ttaatgccaa acttactata tagcagagcg atgaggtaac
    31201 ggatgcgata ttcctcaccg acaatcttgt tcttagagag ttttaattca aagtttctta
    31261 ataaaggaat caatgcttcg cgcatccgat aagctgagga gtttgataaa aaatgacttc
    31321 ttgcaaaatc cgtaagggga cgagagtggg aaccattttt tattaaaaac gctagtaatt
    31381 gtaagacatt agatgatgca tagagttggt aaagataagt ttctttaaaa ggatgtgtaa
    31441 attggcaaga tatcatcctt ttttggatgg tcatagacag actaccaggg aaaaaggcat
    31501 tcagttcctc acaataatgg tttagttgta aaaaggttaa gccagttttt tctgctacct
    31561 cagttattgg caaataagat gtcttaaaaa acaagataat taactgacat tttgattcga
    31621 ttgatgattc caagtatttt tctatcaagg aaagggacct ctcttcctaa acttagaaag
    31681 ccaaataagt ctttgataag tgtattctcc tatttcaatt ctatctaagc taaaaataga
    31741 aatcaaatct aatggttttt ggtgaaaagt cgaaaccttt tgaaatagct ttaaagcaat
    31801 attttctcaa aaaatcattt tcaaaaacat taatttggtg aaaaatataa tcaaaaaatt
    31861 gtctttcttg acaataacgt ggtaagctca tatatgtttt tgagaggaga gaaaatgaat
    31921 aacaaaatat ttttgaataa agaagccggt tttttggcac acacaaaaag aaaaaggcga
    31981 tttgctgtca ctttagtggg agtctttttt atgcttttgg catgcgcggg tgctatcggt
    32041 tttggtcaag tagcctatgc tgcggatgag aagactgtgc cgaattttaa aagcccagat
    32101 ccagattatc cctggtatgg ttatgatgct tatactggag cgtttttaag atatcatgat
    32161 cttagagtca atttaaacgg aagtcggagc taccaagttt attgttttaa tattaaaaaa
    32221 aactatcctc gaccttttac gagtagtgat aaaaaatggt ataaaagatt agagggaact
    32281 gctgaaactt ttaaagttca tgctatggct cccagagtag ggggagagga actaactaaa
    32341 aaattacgga gcgttatgta taacggctac cctaatgacg gcaataatat tatgaaaggt
    32401 ttggagcctt caaatgctat agaagtaaca caatacgcag tttggtacta ttcagataag
    32461 tcacagtatg aaattgagac attatggaag tctgaagcga aagaaggtaa gattagtgag
    32521 tcacaagtta ctttaatgcg tgaagccctg aggaaactaa tttcctctga tgaagatttg
    32581 gttaaacaag ttccatcgaa ctttaaactt agtacttttg agtcttctga taagtcctac
    32641 caaaatcttt taagtgctga gtttgtacct gatgatcccc ctaaacctgg tgatacgtca
    32701 gaacataatc ctaaaactcc cgagttggat ggtagtccta ttcccgaaga cccaaaacat
    32761 ccagatgata atttagaacc tacattgccg ccagtgatgc tagatggtga agaagttcca
    32821 gaagttccaa gcgagagctt agaacctgcg cttcccccat tgatgccaga gctagatggt
    32881 gaagaagtcc cagaagttcc aagcgagagc ttagaacctg cgcttccccc attgatgcca
    32941 gagttagatg gtcaagaagt ccctgaaaaa cctagtgttg acttacctat tgaagttcct
    33001 cgttatgagt ttaacaataa agaccagtca cctctagcgg gtgagtctgg tgagacggag
    33061 tatattaccg aagtctatgg aaatcaacag aaccctgttg atattgataa aaaacttccg
    33121 aatgaaacag gtttttcagg aaatatggtt gagacagaag atacgaaaga gccagaagtg
    33181 ttgatgggag gtcaaagtga gtctgttgaa tttactaaag acactcaaac aggcatgagt
    33241 ggtcaaacaa ctcctcaggt tgagacagaa gacacgaaag agccaggagt attgatggga
    33301 ggtcaaagtg agtctgttga atttactaaa gacactcaaa caggcatgag cggtttcagt
    33361 gaaacagtga ccattgttga agatacgcgt ccgaagttag tgttccattt tgacaataat
    33421 gagcccaaag tggaagagaa tcgggaaaag cctacaaaaa atataacacc tatccttcct
    33481 gcaacaggag atattggaaa tgttttggcc tttcttggaa tccttatttt gtcagtactt
    33541 tctattttta gccttttaaa aaacaaacaa aacaataaag tctgacgtaa aagtgttcca
    33601 taagaaataa tgcgcaaata tgctgaatag tggataactg ctacagtcaa gacaagtgtt
    33661 aattcaaaag gtactactta tacatctgat aataataagg tgacagataa ttcagagcaa
    33721 gtaggatggc taaaaaatga tgtattctat gcataataga cttacaagaa atcaagttat
    33781 tgttaaacgt cgtaaagctt aacgctttca aaagtcgaaa tcaatcgaga ctgtctttag
    33841 gcagtctcga tttttattga taaggaactg caatgacaga aagactaaaa aatctaggga
    33901 tactcttatt atttttattg ggaacagcca tttttcttta ccctacgcta agtagtcagt
    33961 ggaatgccta ccgtgatcgt caattgttat caacttatca caaacaagtg actcaaaaga
    34021 agccctcaga aatggaggag gtgtggcaaa aggctaaggc gtataacgct aggcttggga
    34081 ttcagcccgt tccagatgct ttttcattta gggatggcat tcatgataaa aactacgaaa
    34141 gcctacttca aattgaaaac aatgatatta tgggatatgt tgaagtgccc tctattaaag
    34201 tgacgttacc catataccac tatacgacag acgaggtgct aactaaggga gcaggtcact
    34261 tagttggaag tgccttacca gttggtggcg acgggactca cacggttatt tctgctcata
    34321 gaggccttcc atcggcagaa atgtttacta atttgaattt agtgaaaagg ggagatactt
    34381 tttactttcg tgtgttaaat aaagtattgg cttataaagt tgatcagata ctaactgttg
    34441 aaccagatca agtcacatct ctttctgggg taatgggaaa agattatgct actctggtta
    34501 cctgcacacc atatggtgtt aataccaagc gtctattggt cagaggtcat agaattgcat
    34561 atcattacaa aaagtatcaa caggctaaaa aagcaatgaa actcgtggat aaatcaagaa
    34621 tgtggacaga agtagtatgt gctgctttcg gggtggttat agctattatt ttagtattta
    34681 tgtattcaaa gatgactgat aaaaaagtga ttaaaaacta acttgccctg aaccttcttt
    34741 atcgtattgc aactgtcata aaagtaaaat aatctggtag ttactatccc ctttgatgaa
    34801 gtttaattaa atctcatcaa gagaaaaata ttaggcgatt acaaatgatt ttcccaaaaa
    34861 ttattttttg gaacgcataa tatggaaaaa aagatgataa gaacaactgt tttaaacatc
    34921 aaataaatga tatagtagct atgaaatagc attgtgactg tccggtaaag ggatgggact
    34981 gaagatttta ttcagaagga gagggaatga ataacaaaaa attgcaaaag aagcaagatg
    35041 ctcctcgggt atcaaacaga aagccaaaac aattaactgt cactttagtg ggagtatttt
    35101 taatgctctt agtattgata ggatttgaag gaaaagttcg agcagctcac gaattggttg
    35161 aggtaccagt acctattttt cacaatccag atccacagtc agactatcag tggtatggct
    35221 atgaagccta tacagggggg tatccgaagt atgatttatt caagacatac tatcatgatc
    35281 tgagagtgaa tttgcatggg agtaaaagtt atcaagtata ttgctttaac gttcacaaac
    35341 actatccccg ttcctcccag agttttgatc ggaagtggta taaaaaatta gatggaactg
    35401 ctgagaattt tgattctctt gctatggaac caagagttcg taaagaggag ttaaccaaaa
    35461 aactccgagc tgttatgtat aatgcctatc ctaatgatgc taatggcatt atgaaagatt
    35521 tagagccttt aaatgctatt aaagtcactc aagaggctgt atggtactat tctgattcag
    35581 ctcaaattaa tccggatgaa agttttaaaa cagaagctca aagtaatggt attaatgacc
    35641 agcagttagg cttaatgcga aaagctttaa aagaactaat tgatccaaac ttagggtcaa
    35701 aatattcgaa taaaactcca tcaggttatc ggttaaatgt atttgaatct catgataaga
    35761 ctttccaaaa tcttttgagt gctgagtatg ttccggatac tcccccaaaa ccaggagaag
    35821 agcctccggc taaaactgaa aaaacatcag tcattatcag aaaatatgcg gaaggtgact
    35881 actctaaact tctagaggga gcaactttgc gtttaacagg ggaagatatc ccagattttc
    35941 aagaaaaagt cttccaaagt aatggaacag gagaaaagat tgaattatca aatgggactt
    36001 ataccttaac agaaacatca tctccagatg gatataaaat tgcggagccg attaagttta
    36061 gagtagtgaa taaaaaagta tttatcgtcc aaaaagatgg ttctcaagtg gaaaatccaa
    36121 acaaagaagt aggttctcca tatactatag aggcatacaa tgattttgat gaatttggtt
    36181 tactgtcaac acaaaattat gcgaaatttt attatggaaa aaactatgat ggcagttcac
    36241 aaattgttta ttgcttcaat gccaacttga aatccccacc tgactcggaa gatcatggtg
    36301 ctacaataaa tcctgacttt acgactggtg atattaggta tagtcatatt gctggttcag
    36361 atttgataaa atacgctaat acagctaggg atgaagatcc tcaattattt ttaaaacacg
    36421 taaaaaaagt aattgaaaat gggtatcata aaaaaggtca agctattcca tataacggtc
    36481 tgactgaggc acagtttcgc gcggctactc aactggcaat ttattatttt acagatagtg
    36541 ttgacttaac taaggataga ttgaaagact tccatggatt tggagatatg aatgatcaaa
    36601 ctttgggtgt agctaaaaaa attgtagaat acgctttgag tgatgaagat tcaaaactaa
    36661 caaatcttga tttcttcgta cctaataata gcaaatacca atctcttatt gggacagaat
    36721 accatccaga tgatttggtt gacgtgattc gtatggaaga taaaaagcaa gaagttattc
    36781 cagtaattca tagtttgacg gtgaaaaaaa cagtagtcgg tgagttggga gataagacta
    36841 aaggcttcca atttgaactt gagttgaaag ataaaactgg acagcctatt gtcgacgctc
    36901 taaaaactaa taatcaagat ttagtagcta aagatgggaa atattcattt aatctaaagc
    36961 atggtgacac cataagaata gaaggattac cgacgggata ttcttatact ctgaaagaga
    37021 ctgaagctaa ggattatata gtaaccgttg ataacaaagt tagtcaagaa gctcaatcag
    37081 caagtgagaa tgtcacagca gacaaagaag tcacttttga aaaccgtaaa gatcttgtcc
    37141 caccaactgg ttttactact gatggtggaa cctatctgtg gttattattg cttgtcccat
    37201 ttggtttgtt agtgtggttc tttggtcgta aaggactaaa aaatgactaa ttacctaaat
    37261 cgcttaaatg agaatccact atttaaagct ttcatacggt tagtacttaa gatttctatt
    37321 attggatttc taggttacat tctatttcag tatgtttttg gcgtcatgat tgttaacaca
    37381 aatcagatga gtcctgctgt aagtgctggt gatggagtct tatattatcg tttgactgat
    37441 cgctatcata ttaatgatgt ggtggtctat gaggttgata acactttgaa agttggtcga
    37501 attgccgctc aagctggcga tgaggttaat tttacgcaag aaggaggact gttgattaat
    37561 gggcatccac cagaaaaaga ggtcccttac ctgacgtatc ctcactcaag tggcccaaat
    37621 tttccctata aagttcctac gggtacgtat ttcatattga atgattatcg tgaagaacgt
    37681 ttggacagtc gttattatgg ggcgttaccc atcaatcaaa tcaaagggaa aatctcaact
    37741 ctattaagag tgagaggaat ttaaaaaaat ttggagagag agaaaatgaa aaaaaataaa
    37801 ttattacttg ctactgcaat cttagcaact gctttaggaa cagcttcttt aaatcaaaac
    37861 gtaaaatctg agacggcagg agtgtccgaa aatgcaaaat taatagtaaa aaagacattt
    37921 gactcttata cagacaatga agttttaatg ccaaaagctg attatacttt taaagtagag
    37981 gcagatagtt ctgcaactga taaaacgaaa gatggtttag agattaagcc aggagtaact
    38041 gaaggtttaa ctaccgagca gacaattgct tatgataact cagttaaacc atcggataaa
    38101 tctaagactg caacttttga tttttcaacc gtaaaatttc cagaagttgg tgtttatcgt
    38161 tatacggttt cagaaataga tagcaaagtt agtgggatta aatacgatac caaaacatgg
    38221 atagtggatg tttatgttgt taatgatggt aatggaggat ttaaggcacg gtatattgta
    38281 tctaaagaga agggacaaaa tgataaaaag cctgttgttt ttgaaaactc ctttaagaca
    38341 acttctttaa aggtagaaaa acaggtgact ggtaatacag gagaactaaa gaaagacttc
    38401 aatttcactt tgacaattaa tcctaatgat aatttcgtag ctggacaagt tattaaatta
    38461 gagaaaggtg gaattaaagc tgatgttaag attggtgagc cgtataaatt tgctctaaag
    38521 aatggtgaga aagtaacttt atcaaaatta ccagtaggtg ttacttatag catcattgaa
    38581 gatgaggcag ataaagacgg atacacaact aatgccaaaa ttacagatgg tacggcagcg
    38641 cctgtggaat ataagttagg aaatcaacaa ttggctgacg aatctgctga cgaaatcgtt
    38701 gtcacaaata accgtgacac tcaagttcca actggtgttg taggcaccct tgctccattt
    38761 gcagttctta gcattgtggc tattggtgga gttatctata ttacaaaacg taaaaaagct
    38821 taagtgatta gtcaaagaat gatgatgaca attgtacagg ttatcaataa agccattgat
    38881 actctcattc ttatcttttg tttagtcgta ctatttttag ctggttttgg tttgtgggat
    38941 tcttatcatc tctatcaaca agcagacgct tctaatttca aaaaatttaa aacagctcaa
    39001 caacagccta aatttgaaga cttgttagct ttgaatgagg atgtcattgg ttggttaaat
    39061 atcccaggga ctcatattga ttatcctcta gttcagggaa aaacgaattt agagtatatt
    39121 aataaagcag ttgatggcag tgttgccatg tctggtagtt tatttttaga tacacggaat
    39181 cataataatt ttacggacga ttactctctg atttatggcc atcatatggc aggtaatgcc
    39241 atgtttggcg aaattccaaa atttttaaaa aaggattttt tcaacaaaca taataaagct
    39301 atcattgaaa caaaagagag aaaaaaacta accgtcacta tttttgcttg tctcaagaca
    39361 gatgcctttg accagttagt ttttaatcct aatgctatta ccaatcaaga ccaacaaagg
    39421 cagcttgttg attatatcag taaaagatca aaacaattta aacctgttaa attgaagcat
    39481 catacaaagt tcgttgcttt ttcaacgtgt gaaaattttt ctactgacaa tcgtgttatc
    39541 gttgtcggta ctattcaaga ataacgaaag gaggagactt ttgagaaaat attggaaaat
    39601 gttattttct gtcgtaatga tattaaccat gctggccttt aatcagactg ttttagcaaa
    39661 agacagcact gttcaaacta gcattagtgt cgaaaatgtc ttagagagag caggcgatag
    39721 taccccattt tcgattgcat tagaatcaat tgatgcgatg aaaacaatag aagaaataac
    39781 aattgctggt tctggaaaag caagcttttc ccctctgacc ttcacaacag ttgggcaata
    39841 tacttatcgt gtttatcaga agccttcaca aaataaagat tatcaagcag atactactgt
    39901 atttgacgtt cttgtctatg tgacctatga tgaagatgag actctagccg caaaagttat
    39961 ttctcgaagg gctggagacg aagaaaaatc agcgattact tttaagccca aacggttagt
    40021 aaaaccaata ccgcctagac aacctgacat ccctaaaacc ccattaccat tagctggtga
    40081 agtaaaaagt ttattgggta tcttaagtat cgtattactg ggattactag ttcttcttta
    40141 tgttaaaaaa ctgaagagta ggctataaaa cccattttta agagtcacac atttttagtt
    40201 gctctatttt tataatgaaa cttattgaca tctccatagt cttgcttgac tttggagatg
    40261 ttttctatgc tgataggttt ttgtaccata taagataatg attgatgaaa agagtgtctt
    40321 gaattcttaa atattatctc ggtatttagc aagatagtct tttgaagaaa tacccgtgta
    40381 ttttttaaag attttatgaa aatgggtagt gtcataaaaa tataagcttt tagccacttc
    40441 tccaacagga attcctcgtt ttaagagtag ttgagcttct ttgatttttg tacttagaat
    40501 ataatgttgt aaggaaacat ttgagtattt tttaaagact gaacgtaagt gagattcgga
    40561 aacatatagg cgcttagcga tatcagatac ctttaatttg gaatacaggt gactatcgac
    40621 ataatgaagc acactattgt acatttgacg tttatcagaa atacttttat tggttaatga
    40681 ttcggaaaaa tggatgatag ctgcacagcg aattctcaaa actttgagca agtcactcgc
    40741 ttcttcattt aaggccatgt agtatttgac aaggctataa tgctttgcta cgtcagtatt
    40801 ttctacaaaa ctgaattcca gtaacctagt taaatagata acagtgaggt tcttttccgc
    40861 ccttagagca gaaatagaag atgaggtgat acttgataag gggatgcgat tgatttcttg
    40921 cttgagcaat tgtggattgc ctagctttac cagatccata atatgattta ggtgctctaa
    40981 aacaggcggt aattggctag atgctttgct aatatgctta aggcgatgaa tgatatcagg
    41041 atcagcagtc atctgttttg aatgctgcaa caattgatga attgtttgtt ggcaagtcgt
    41101 ctcaaattgt gtgtcaaaaa aagcgtcaat agctatcaaa aggttccgca cattattaat
    41161 tggaaaatgt gggacaagtg ccatatagga caagagctct tctttgctac gatgagaaga
    41221 atgttttaaa aaattattag ctaattgttc ctgatagtct ttattaagtg aataagggta
    41281 gaaaggtcca atggcaataa tgtagtgaca aagaggaaaa atcataaagg actcttcaaa
    41341 taaaccctca taaaaaataa catcttgtgc ggcttgagga atagctagat gctttaaaaa
    41401 ttgatagtaa cataataaat agtcgtcatt accatatact tgaataagtg ccttatcttg
    41461 tgacatcact gaaataggta attgagacaa gctgtgtaat gtttgcacat attttaaatc
    41521 gaatataacc atttgataga ttgcccccca tccttagttg tatcattttc cttagacttg
    41581 taaaagcttt agtaaatgtt aatgatgaaa attcctattt ggtaggcaca ctaaaaggtt
    41641 ttcctaatag taaactctct ctccatcatc tcctacctta attatactat aaatagctaa
    41701 gcagataaag ataaaggatt tgtacgttaa gcgcttgaaa aagaaagagt tacagataat
    41761 gacataaaaa acgctaaaaa accatcaaaa taaatactct gaccataaga tgtagacttg
    41821 acaactgaaa atggtaaaat aactatttga cagttgtcct gtagtcttta gttttggaca
    41881 taggctgtcg cttatgaatg tggagagaga aaataaatga cacaaaaaaa tagctataag
    41941 ttaagcttcc tgttatcctt aacaggattt attttaggtt tattattggt ttttatagga
    42001 ttgtccggag tatcagtagg acatgcggaa acaagaaatg gagcaaacaa acaaggatct
    42061 tttgaaatca agaaagtcga ccaaaacaat aagcccttat cgggagcaac tttttcactg
    42121 acaccaaagg atgggaaggg aaaacctgtt cagaccttta cctcaagcga agagggtatt
    42181 atagatgctc aaaatctcca accagggact tataccttaa aagaagataa agcaccagat
    42241 ggttatgata aaaccagtcg gacttggaca gtgactgttt atgagaacgg ctataccaag
    42301 ttggttgaaa atccctataa tggggaaatc atcagtaaag cagggtcaaa agatgttagt
    42361 agttctttac agttggaaaa atcaacaatg tctgtattct ctaaatacgg agggacagaa
    42421 gtaactagtg gcgcagcgga tttctaccgc aatcatgccg cctattttaa aatgtctttt
    42481 gagttgaaac aaaaggataa atctgaaaca atcaacccag gcgatacctt tgtgttacag
    42541 ctggacaaac gtctcaatcc taaaggtatc agtcaagata tccctaaaat catttatgac
    42601 agtgaaaata gtccgcttgc gattggaaaa tacgatgcca agactcatca gctcacctat
    42661 acttttacaa attatatcgc tggtttagat aaagtccagt tgtctgcaga attgagctta
    42721 ttcctagaga ataaggttgt tttagaaaat caaacgatat cagattttaa gagtaccata
    42781 ggtgatcagg aaatcaaata tggtgggaca gttaatgtcc tttatggaaa tgcatctggg
    42841 caagacaaca attatgttac taacggattg agcaatgtgg gtggaagtat tgaaagctac
    42901 aatacacaaa caggcgactt tacttggtat gtttatgtca acccaaatcg taccaatatt
    42961 ccttatgcga ccatgaattt atggggattt ggaaggttgg ttaaatctaa taactcaaca
    43021 agtaagtcag aggattccgc tacaagtagt gcccaattgg aaaaagtaga aatttatgaa
    43081 gttaaagagg actatacgct acccactagt tatggggttg atctaacaaa attgaattta
    43141 agaaaagacc ttgaggcaaa gttaccacaa gatagtactc aaaattctaa taagcgcttg
    43201 cgaattgact ttggagataa acttcaaaat aaagcctttg ttgttaaaat aacagggaaa
    43261 acggatcaat ctggtaagcc actagtcgtt caatccaatt tggccagttt taataatgaa
    43321 actgctgtaa cttatggtaa tcctgttaat aacgtctact tccaaaacga aattgccttg
    43381 tctccttcta agggtagtgg ttctgggaca agtgaattta ctaagccctc tattacagta
    43441 gcaaatctaa aacgagtggc tcagcttcgc tttaagaaaa tgtcaactga caatgtgcca
    43501 ttgccagaag cggcctttga gctgcgttca tcaaatggta atagtcaaaa agtagaagca
    43561 aaatcaaaca cacaaggaga ggttcatttt aaggacctga cctcgggcac ctacgacttg
    43621 tatgaaaaac aagcacctaa aggctatcag caggtgacag agaaattggc gaccgttact
    43681 gttgatacta ccaaacctgc tgaggaaatg gtcacttggg gaagcccaca ttcgtctgta
    43741 aaagtagaag atagcaaaga agtcacgatt gtcaaccata aagaaaccct tacgttttca
    43801 gggaagaaaa tttgggagaa tgacagacca gatcaacgtc ctgcaaagat tcaagtgcaa
    43861 ctgttgcaaa atggtcaaaa aatgtccgat aaagttcaag aagtaacgaa ggataacgat
    43921 tggtcttatc acttcaaaga cttgcctaag tacgatgaca ggaatcagga gtataagtac
    43981 tcagttgaag aagtgaaagt tccagacggc tacaaggtgt cgtatttagg aaatgatata
    44041 tttaacacca gagaaacaga atttgtgttt gaacagaata actttaacct tgaatttgga
    44101 aatgctgaaa taaaaggtca atctgggtca aaaatcattg atgaagacac gctaacgtct
    44161 ttcaaaggta agaaaatttg gaaaaatgat acggcagaaa atcgtcccca agccattcaa
    44221 gtgcagcttt atgctgatgg agtggctgtg gaaggtcaaa ccaaatttat ttctggctca
    44281 ggtaatgagt ggtcatttga gtttaaaaac ttgaagaagt ataatggaac aggtaatgac
    44341 atcatttact ctgttagaga agtaactgtt ccaacaggtt atgatgtgac ttactcagct
    44401 aatgatatta ttaataccaa acgtgaggtt attacacaac aaggaccgaa actagagatt
    44461 gaagaaacgc ttccgctaga atcaggtgct tcaggcggta ccactactgt cgaagactca
    44521 cgcccagttg ataccttgtc aggtttatca agtgagcaag gtcagtccgg tgatatgaca
    44581 attgaagaag atagtgctac ccatattaaa ttctcaaaac gtgatattga cggcaaagag
    44641 ttagctggtg caactatgga gttgcgtgat tcatctggta aaactattag tacatggatt
    44701 tcagatggac aagtaaaaga tttctacctg atgccaggaa aatatacatt tgtcgaaacc
    44761 gcagcaccag acggttatga gatagcaact gctattacct ttacagttaa tgagcaaggt
    44821 caggttactg taaatggcaa agcaactaaa ggtgacgctc atattgtcat ggttgatgct
    44881 tacaagccaa ctaagggttc aggtcaggtt attgatattg aagaaaagct tccagacgag
    44941 caaggccatt ctggctcaac tactgaaata gaagacagta aatcttcaga ccttatcatt
    45001 ggcggtcaag gtgaagttgt tgacacaaca gaagacacac aaagtggtat gacaggccat
    45061 tctggctcaa ctactgaaat agaagacagc aagtcttcag acgttatcat tggcggtcag
    45121 gggcaggttg tcgagacaac agaggatacc caaactggca tgcacgggga ttctggttgt
    45181 aaaacggaag tcgaagatac taaactagta caatccttcc actttgataa caaggaatca
    45241 gaaagtaact ctgagattcc taaaaaagat aagccaaaga gtaatactag tttaccagca
    45301 actggtgaga agcaacataa tatgttcttt tggatggtta cttcttgctc acttattagt
    45361 agtgtttttg taatatcact aaaaaccaaa aaacgcctat catcatgtta agataagctg
    45421 ttggtgacaa tagcgaaaaa gggtccactt gttagtggac ctctttttct ggaatcgatc
    45481 gtctcggttc actagataag tcattttaag tagttcaaaa aggcagagcc ttaaaaagtt
    45541 ttctgccttt ttaatcttgt ggggcactta cgaaagtatt tcggccactt caagcataat
    45601 aaaaagagtc tgagcaatca tgttgtctca gactctgact gtaattatgc aaaagatgat
    45661 ggactggcag cttcatttac tcaccgaact cccctttata acttgggaat gatgctgcta
    45721 atgggttgtt ttgtttaata cctgctataa acggtaattt atcccattta tctttgtaat
    45781 tatcttcaag ctcagtttta aacttccaaa ccattttatg agaaaaataa gtgtgcgatt
    45841 gacggccgta ggtgtctact aaaacgtgag aaaattttcc gtcgtttttt agtttgtaaa
    45901 atttatctag agaaggactc cagccagtat aaccagggaa tttatcaaat tcttgattaa
    45961 aatttgtttg ttggcttccc caatcataat gccattgtgt attattgtgt tttttagaat
    46021 caactgtacc gttcccataa tgatgctcat aagttatctc cgtaggagct tttacaccct
    46081 taggaagaaa cactgagaaa tcagggtaaa cccattgttc tttagctgaa ttaaaggtta
    46141 cagtccattc gatactgtta ttgttatcga ccaatgtttt agtatagtcg acatagttat
    46201 caacctagta atttccctgc cagtaaaggt gcactttacc tttttcatca atgttatcat
    46261 gggcatgtac tgatttatac gaactaaaca gcatcgaagc tgctagtagt gaagtgaaga
    46321 gatatttatt acatcgtttc a
//
LOCUS       DATUYR010000009        44575 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.009, whole genome shotgun sequence.
ACCESSION   DATUYR010000009 DATUYR010000000
VERSION     DATUYR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44575
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.009"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            192..1424
                     /locus_tag="VCI33_000422"
     CDS             192..1424
                     /locus_tag="VCI33_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP2831365.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDTKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1592..2773
                     /locus_tag="VCI33_000423"
     CDS             1592..2773
                     /locus_tag="VCI33_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP2831366.1"
                     /translation="MTKRIILTIFTFICFSVMPLVQAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2925..4343)
                     /gene="sufB"
                     /locus_tag="VCI33_000424"
     CDS             complement(2925..4343)
                     /gene="sufB"
                     /locus_tag="VCI33_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEP2831367.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4336..4815)
                     /locus_tag="VCI33_000425"
     CDS             complement(4336..4815)
                     /locus_tag="VCI33_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEP2831368.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4802..6028)
                     /locus_tag="VCI33_000426"
     CDS             complement(4802..6028)
                     /locus_tag="VCI33_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP2831369.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6059..7321)
                     /gene="sufD"
                     /locus_tag="VCI33_000427"
     CDS             complement(6059..7321)
                     /gene="sufD"
                     /locus_tag="VCI33_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEP2831370.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKTSANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVHQEIIKVLDEKLLNR"
     gene            complement(7416..8186)
                     /gene="sufC"
                     /locus_tag="VCI33_000428"
     CDS             complement(7416..8186)
                     /gene="sufC"
                     /locus_tag="VCI33_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEP2831371.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADKDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8308..9477)
                     /locus_tag="VCI33_000429"
     CDS             complement(8308..9477)
                     /locus_tag="VCI33_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEP2831372.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9484..10245)
                     /gene="mecA"
                     /locus_tag="VCI33_000430"
     CDS             complement(9484..10245)
                     /gene="mecA"
                     /locus_tag="VCI33_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEP2831373.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10391..11230)
                     /locus_tag="VCI33_000431"
     CDS             complement(10391..11230)
                     /locus_tag="VCI33_000431"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEP2831374.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKRNAQQEPAVTELARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11296..13221)
                     /locus_tag="VCI33_000432"
     CDS             complement(11296..13221)
                     /locus_tag="VCI33_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEP2831375.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRAGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGEEPV
                     DVSTSKKNKIVALETKISRRRALLQLLFGKVIPLVEVGFLLWQLIQFIRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLVLNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEIYQAVRPGELSMVMYATTPTFLSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13419..14987
                     /locus_tag="VCI33_000433"
     CDS             13419..14987
                     /locus_tag="VCI33_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP2831376.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYADATSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAILKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14980..15720
                     /locus_tag="VCI33_000434"
     CDS             14980..15720
                     /locus_tag="VCI33_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2831377.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDHIIFMDKGL
                     ITEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15862..15978
                     /locus_tag="VCI33_000435"
     CDS             15862..15978
                     /locus_tag="VCI33_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEP2831378.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16204..17214)
                     /gene="gap"
                     /locus_tag="VCI33_000436"
     CDS             complement(16204..17214)
                     /gene="gap"
                     /locus_tag="VCI33_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEP2831379.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17562..19640)
                     /gene="fusA"
                     /locus_tag="VCI33_000437"
     CDS             complement(17562..19640)
                     /gene="fusA"
                     /locus_tag="VCI33_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEP2831380.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(20007..20477)
                     /gene="rpsG"
                     /locus_tag="VCI33_000438"
     CDS             complement(20007..20477)
                     /gene="rpsG"
                     /locus_tag="VCI33_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEP2831381.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20498..20911)
                     /gene="rpsL"
                     /locus_tag="VCI33_000439"
     CDS             complement(20498..20911)
                     /gene="rpsL"
                     /locus_tag="VCI33_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEP2831382.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21119..23740)
                     /locus_tag="VCI33_000440"
     CDS             complement(21119..23740)
                     /locus_tag="VCI33_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEP2831383.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKV
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEVLAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQETLAALQAKQSSLEATIATTEHQLTLLKTLANEKGYRHLDED
                     IATVPDLQVAPPLTGVKPLSYSKIDTTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESK"
     gene            complement(23731..24570)
                     /gene="purR"
                     /locus_tag="VCI33_000441"
     CDS             complement(23731..24570)
                     /gene="purR"
                     /locus_tag="VCI33_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEP2831384.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(24837..25775)
                     /locus_tag="VCI33_000442"
     CDS             complement(24837..25775)
                     /locus_tag="VCI33_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEP2831385.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVTEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(25765..27036)
                     /locus_tag="VCI33_000443"
     CDS             complement(25765..27036)
                     /locus_tag="VCI33_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEP2831386.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     ITYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEEINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQTTKSLLNMPLLEEENNEN"
     gene            complement(27038..27670)
                     /locus_tag="VCI33_000444"
     CDS             complement(27038..27670)
                     /locus_tag="VCI33_000444"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEP2831387.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(27663..28325)
                     /locus_tag="VCI33_000445"
     CDS             complement(27663..28325)
                     /locus_tag="VCI33_000445"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEP2831388.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGVDIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            complement(28335..29207)
                     /gene="rsgA"
                     /locus_tag="VCI33_000446"
     CDS             complement(28335..29207)
                     /gene="rsgA"
                     /locus_tag="VCI33_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEP2831389.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(29631..30503)
                     /gene="rsmA"
                     /locus_tag="VCI33_000447"
     CDS             complement(29631..30503)
                     /gene="rsmA"
                     /locus_tag="VCI33_000447"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEP2831390.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKD
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKTKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(30617..31207)
                     /gene="rnmV"
                     /locus_tag="VCI33_000448"
     CDS             complement(30617..31207)
                     /gene="rnmV"
                     /locus_tag="VCI33_000448"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEP2831391.1"
                     /translation="MQRGYLSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            complement(31179..31970)
                     /locus_tag="VCI33_000449"
     CDS             complement(31179..31970)
                     /locus_tag="VCI33_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEP2831392.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALALADQYPNVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKETTANAKRVLKLD"
     gene            32255..33085
                     /locus_tag="VCI33_000450"
     CDS             32255..33085
                     /locus_tag="VCI33_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP2831393.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNGL
                     "
     gene            complement(33196..34134)
                     /locus_tag="VCI33_000451"
     CDS             complement(33196..34134)
                     /locus_tag="VCI33_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP2831394.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMVQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(34156..35070)
                     /locus_tag="VCI33_000452"
     CDS             complement(34156..35070)
                     /locus_tag="VCI33_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEP2831395.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIVDKDLEKAKTLQYTINETIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            complement(35082..35744)
                     /locus_tag="VCI33_000453"
     CDS             complement(35082..35744)
                     /locus_tag="VCI33_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEP2831396.1"
                     /translation="MTSKKQGLLHSLFKLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSHFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELPLLILFKRSLLLAGILFP
                     WTFAFLVVIGLLVFTLPLTLLTLFCGLSLLATMGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(35901..36731)
                     /locus_tag="VCI33_000454"
     CDS             complement(35901..36731)
                     /locus_tag="VCI33_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP2831397.1"
                     /translation="MTKKKLTASDVLTTVILCILTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(36744..37631)
                     /locus_tag="VCI33_000455"
     CDS             complement(36744..37631)
                     /locus_tag="VCI33_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2831398.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIAILLTTSVGQPIILYIAAMGNIDNSLMEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(37734..39053)
                     /locus_tag="VCI33_000456"
     CDS             complement(37734..39053)
                     /locus_tag="VCI33_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP2831399.1"
                     /translation="MNMKKLASLVMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTEEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            complement(39102..39806)
                     /locus_tag="VCI33_000457"
     CDS             complement(39102..39806)
                     /locus_tag="VCI33_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEP2831400.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMAEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            complement(40080..40214)
                     /gene="rpmH"
                     /locus_tag="VCI33_000458"
     CDS             complement(40080..40214)
                     /gene="rpmH"
                     /locus_tag="VCI33_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEP2831401.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            complement(40529..41443)
                     /locus_tag="VCI33_000459"
     CDS             complement(40529..41443)
                     /locus_tag="VCI33_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEP2831402.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPAVNSADVEPEEIKATQKLEAED
                     TKVVPLMSEDSPAQTLSNLAETVTETKAQQPSIPVEESEVPQDPGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            complement(41455..42282)
                     /locus_tag="VCI33_000460"
     CDS             complement(41455..42282)
                     /locus_tag="VCI33_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP2831403.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            complement(42248..42607)
                     /gene="rnpA"
                     /locus_tag="VCI33_000461"
     CDS             complement(42248..42607)
                     /gene="rnpA"
                     /locus_tag="VCI33_000461"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEP2831404.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            complement(43147..43887)
                     /locus_tag="VCI33_000462"
     CDS             complement(43147..43887)
                     /locus_tag="VCI33_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2831405.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            complement(43891..>44575)
                     /locus_tag="VCI33_000463"
     CDS             complement(43891..>44575)
                     /locus_tag="VCI33_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEP2831406.1"
                     /translation="IVKLDRFAKDQLSQKLTQAQNERIAYLENYNQSIEQLYREIRTV
                     KHDSENILISLKDSIDSGDIDLITRVYDTVIQQSATSMMRTNYEISSLDNIKEAVIRS
                     IMNSKLLEAQYLGIELYIEIPDVIDHLPIKLIDLIVLFTGLVDNAIETAKGSRRPFLS
                     IAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQQQNSAHFLTVLDSYPQITLSTKSDHY
                     RLRQLLEMR"
BASE COUNT        13315 a         9528 c         8147 g        13585 t
ORIGIN      
        1 ttattttata catttatcta tatgaaagcg ttatactcct tttttaatga ggtttcaatt
       61 agtttaataa agattgtcat tctctcatgg tagcgtttta ttgcatcact actcttgtga
      121 ggtaagtcat taccttagac aaaaatatgg tataattaaa aaaataatac ttgagtaagg
      181 ataatctata aatgatcaaa cgattaattt ccctagtggt catcgcctta ttttttgcag
      241 caagcactgt tagcggtgaa gagtattcgg taactgctaa gcatgcgatt gccgttgacc
      301 ttgaaagtgg caaagtttta tacgaaaaag atactaaaga agttgtccca gtcgcctcag
      361 tcagtaagct cttgacaacc tatctggttt acaaagaagt ttctaagggc aagctaaatt
      421 gggatagtcc tgtaactatt tctaactacc cttatgaact cactacaaac tatactatta
      481 gtaacgttcc tcttgataag agaaaatata ccgttaaaga acttttaagt gcgttagttg
      541 ttaataacgc caatagcccc gctattgctt tagctgaaaa aataggcgga accgaaccca
      601 aatttgttga caaaatgaaa aaacaattaa ggcaatgggg catttccgat gcaaaggtcg
      661 ttaattcaac tggcttaact aaccattttt taggagctaa tacttatcct aatacagaac
      721 cagatgacga aaattgtttt tgcgccactg atttagctat tattgccagg catctcttat
      781 tagaatttcc agaagtactg aaattatcta gcaaatcctc cactattttt gatggacaaa
      841 ccatttacag ttataattac atgcttaaag gcatgccttg ttatcgagaa ggcgtggatg
      901 gtctttttgt tggttattct aaaaaagccg gtgcttcttt tgtagctact agtgtcgaaa
      961 atcaaatgag ggttattaca gtagttttaa atgctgatca aagccacgag gatgatttag
     1021 ctatatttaa aacaaccaat caattgttgc agtacctttt aattaatttt caaaaagtcc
     1081 agttaattga aaataataaa ccagtaaaaa cgttatctgt cttagacagt cctgaaaaaa
     1141 ctgtcaaact tgtagcccaa aatagtttat tttttatcaa accaatacat acaaagacca
     1201 aaaataccgt ccatattact aagaaatcat ccacaatgat cgcacctcta tcaaagggac
     1261 aagtcttagg tagagcaacc cttcaagata aacatcttat tggacagggt tatctggata
     1321 ctcctccttc tatcaatctt atccttcaaa aaaacatttc taaaagtttc tttttaaagg
     1381 tctggtggaa ccgttttgtg aggtatgtca atacctcttt atagaataac acaatgcttt
     1441 ttgtatgaca ttgactgaca gagtacttct gcagtctttt tcatttttat ttaagcaaac
     1501 taggccaatc ctaacaattt ttcaaaaaaa cttcacattg tattagcaaa gtgatatact
     1561 gaaaatgcat tacatttaaa ggagtcggaa attgaccaaa agaattatac taactatttt
     1621 tacctttatt tgcttttcgg tgatgcctct tgtccaagcc gaagatgtca tggatattac
     1681 aaggcaagca ggctacacag ttagtgaggt taatcgacca aaatcttcta ttgtcgttga
     1741 tgcaaatagt tcagatattc tttggcaaga taatattgat attcctaggg atcctgctag
     1801 tatgtctaaa atgtttacgc tttacatttt atttgaagag ctcgctaagg gaaaaattac
     1861 gatggacact actattacgg caactccaac cgatcaagct atcgctaaca tttatgagat
     1921 tagcaataat aatatcgtcg ctggagtagc ttatcctatt cgtgatctta ttactatgac
     1981 agctgtgcct tcatcaaatg cagcaactgt tatgattgcc aattatttat caaacaatga
     2041 tgcttctgcg tttatcgatc gtattaatgc cacagccaaa caattaggca tgactaacac
     2101 tcatttttca aacgctagtg gtgcagcagc acaagctttt caaggttatt ataatcctac
     2161 taaatatgat ttatctgctt caaatattac gaccgctcga gatttgtcca agttactcta
     2221 tgccttctta aaaaaatacc ctgaaattat ctcctttaca aataaatctg ttgtgcacac
     2281 tatggtagga acgccatatg aagaagaatt tcacacctat aaccactctc tcccagacaa
     2341 tcaatttggt atgaaaggag ttgatggatt aaaaacaggt tctagtccca gtgctgcttt
     2401 taacgccatg ataactgcca aaagaggcaa aactcgactc attactattg tcatgggggt
     2461 cggagattgg tcagatcaaa acggagagtt ttatagacat cctttcgtta atgccttgac
     2521 tgagaaaggg tttaaagata gtaagacctt atccaaaaaa gcacgccaaa aactcgaaaa
     2581 attagtgcca caaaccaaaa aagaaacatc acctaaacag cagcacttca aagccactaa
     2641 aaagcaatcg tacttggaaa gagtcgaaga ttttatgaat cacaaccaca ctttcttgct
     2701 tatttgctta gctatcttta taattactat ccttttacta agcctcgttg tctttgcaat
     2761 gggacgccaa taatgaatta tcaaaaacct ctgtcaaagc acagaggttt ttgatatgtt
     2821 atcttacgtg ttcaaggcaa ctaaaaacta accacttggg gttagtacta caactcataa
     2881 aatcctatct caaagatctt tcagatggat aagagaaaat gcaattaacc gacagaacct
     2941 tccatctcat aggaaatcaa tcgattgagt tcgaccgcat attccattgg caattctttg
     3001 gtaaaaggct ccacaaatcc catgacaatc atctctgtgg cttcgctttc tgataagcca
     3061 cggctcatga gatagtagag ttgttcttca gaaatcttag acactttagc ttcatgttct
     3121 aacgccacct gtgaattatg aatttcgtta aatgggatcg tatctgattt agaaatatcg
     3181 tccatcaaaa tagtatcaca ttcaatatgg gaaacggatt ttttagattg cttgttaaag
     3241 gtcacttggc cacgataatc tactttacca ccagacttgg caattgattt tgacacaatt
     3301 gatgatgatg tatggggagc attgtgaatc attttagccc ctgtatcttg gtgttggcct
     3361 gcgttagcaa aggcaataga cagcatggtg ccacgcgcac ctggcccatc aaggtaaaca
     3421 gaagggtact tcatggtggt tttagctcct agattgccat caatccattc cactgttgca
     3481 tccgtaaggg cgcgtgcacg ttttgttact agattataca cattgtctga ccagttttga
     3541 atggtggtat aacgcatgta tgcaccgtca agcgcaaaga tttcaacgat agcagcatgt
     3601 aagctgttac ttgaataagt gggagctgta catccttcaa cataatgaac acttgctcct
     3661 tcatcaacaa taatcaatgt ccgctcaaat tgaccagtat tttcattgtt aatgcggaaa
     3721 taagtttgca aagggatatc taccttgacc cctttaggaa cataaataaa ggtaccacca
     3781 gaccaaactg ctgaattgag ggctgctaat ttgttgtctg ttggcggaac cagtttggca
     3841 aagtattgtt tgaacaaatc aggatattct ttgagggcgg aatcggtatc tgtaaagata
     3901 atccctagct tttcaaattc acccttcatg ttatggtaaa ccacttctga ctcatactga
     3961 gctgatgccc cagcaagata agcacgttct gcttctggaa ttccaatacg atcaaaggtt
     4021 tcttttattt tttctggaac atcatcccaa gaacgagctg gcttatcaga tgctttttga
     4081 tagtaaatga tatcatcaaa gttaatatct gataagtctg ctccccaggt ttgcatcggc
     4141 attttattaa aggtttccaa ggattttaaa cgaaactcca acatccactc aggttcattt
     4201 ttggcagctg atagttcgcg aaccactgcc tcactcaatc cttttcctgt agaataaatg
     4261 ggctctacat catcgtgaaa tccaaattgg tagtccccta agtcaattgg ctttggctct
     4321 actttctcat ttatatcaga catttttccc ttcctttaca ttttggtccg tgaggtgctg
     4381 agcattggca cttcgtttaa tggcttcctt gagagcattc caagctaggg tagagcattt
     4441 gatacgctgt ggaaattttg caactcctgc caacaattct gcttcaccta gctctttttg
     4501 agcaggattt tcctgtcctt gtaccatctc tgaaaaaata tcagctaacg cgagtgcttc
     4561 ttctttactt ttaccaataa cagcatctgt catcatgctt gatgaagctg tggaaatggt
     4621 acagccgttg cctgcaaaag caatatcttc aattttatct tcgtcaaact taacggtcaa
     4681 agaaatcaca tcaccacaag tcggattatt cagttgaaca gcctctacgc catctagttg
     4741 cccatgatga tgtggacgtt tcgaatggtc cgctaccaca gccatgtata gatggttcag
     4801 tttagagagt gccattgaaa aactcctttg cttttagaat tgcttcgact agacggtcac
     4861 aatctgcctt ggtgttatag atataaaaac ttgctctaac agttgctggt acacctaaat
     4921 aactaagaag aggttgggcg cagtggtgcc ctgctcttac tgcaacacct tcatagtcca
     4981 aggctgttgc caagtcatga ggatgcaaat catccagatt aaaagaaatc agaccagatc
     5041 ttgcactagg ctggcttggt ccatatatgg taagcccctc aatagcttct aatttcggca
     5101 agacataggc tattagttct gcttcatgcg catgtatatc agccatgcct agtctctgaa
     5161 ggtaagaaat ggctgcgctt agcccaatag caccagctat gtgaggtgtt cctgcttcaa
     5221 acttccaggg caattctttc caagtggcct cttgttcgta aacaaaatca atcatttctc
     5281 cgccaaattc aagaggctcc acttgattca aaagctcttc tttgccgtaa agaacaccca
     5341 aacctgttgg ccccaacatc ttatgagctg aaaaagcaaa gaaatcacaa tccaagtctt
     5401 gaacatcaat agccaaatgt ggaaccgact gggcaccgtc aacaacaagg taggctcctt
     5461 tagcatgtgc cagcttggca atttctttaa tgggattgat gcaaccaaga acattggaga
     5521 catgtactag gctaacaaaa cgtgtttttg tcgtcaattt gtttgccaaa tcgtccatgt
     5581 caagttggcc atcttttaaa taaacataga ctaaccttgc tcctgttttt tgacaggctt
     5641 gttgccaagg aatgatattg gcatgatgct ccataatcga aatcaacacc tcatcttctg
     5701 gcgtcaagac ctgctctgca aaccgagcaa cccaattcaa actggttgtt gttcctctgg
     5761 taaagagcac ttccttactt gatttagcat gaataaagtc agcaacctgc tggcgactgg
     5821 cctcatattt gtgcgttgca cgttcagcca aggtatgaac tcctcggtgg acattagcat
     5881 tatcttcttg ataataggat tgcaaagcct caagaacaag cgccggtttt tgtgtggtgg
     5941 cggcattatc caaataaaca aggggttcat cattgacttg ttgatttaag atttgaaagt
     6001 cttgtttgat gtcttttgcg tctagcaata ctaacctctt tctttttggc agtaggtctt
     6061 aacgattaag caatttctca tctaaaacct taataatctc ttggtggact gatggaatag
     6121 gaatttcagc gataactgct cctaggaatc ctctaataac caatcgttct gctgtttctt
     6181 gatccagtcc tcgactcatc aagtaataca tatcttcagg gtcaacctga ccgatagaag
     6241 ctgcatgacc tgctgttact tcattttcat caattaagag gattggattg gcatcggctc
     6301 ttgcttggtc agaaagcatc aaaacacggc tttcttgttg agcatcagct cccttagcgc
     6361 cttttagaat atgaccaatc ccgttaaacg ttaaggtgcc acgttccaaa atcacaccat
     6421 gctgtaaaat atgaccgacc gtacgttgac catagttggt cacgcgcgtg tcaatacctt
     6481 gtacctgacg accacttgag gctgcaacaa ctttcaaatc agcttgtgag ccctgaccaa
     6541 tcaaatcact gtcaaaatca gcaatgacat tgccttcatt catcacagct aaggcccaat
     6601 caatgttggc atccttctct aaacgtcctc gacggctaat ataggttgtc actgaaggac
     6661 ctaagcggtc gatagccgag aatttaatct ggctgcctgc ttgggcaatc acttctacac
     6721 tgatattagc gctggtcttt tgagtggcat tgccaataga ttcaaaacgc tctaaatagg
     6781 tgaacttact ttcttttcct gcaatcacta gaacatgctt gttaaaagga acgtcactgt
     6841 cactatcttg taagaaaata gcttcaatag gagttgtgat ttccaagtga tcaggaacgt
     6901 agagcacggc tgcgctatta aaataagcag tgtggtaggc agctagtttg tcttcatcaa
     6961 aagctaatgc ctgaccaaaa tgagcttcaa ttacttctgg gatttcctca agcgccgtat
     7021 aaaaatcact gaaaacaact cccttgtcaa ttaacgccat tggtaactgt tctaagactg
     7081 tttgcgtgcc tacctgaaca agctttgggt tatctccaat agctataaaa tctggaacac
     7141 tagctagact ttcattttct gttaaggtac catctcctag attccaacgg tgaaatttaa
     7201 ccctttcgat ggttggtaac tctaattctg gtatggcttc taatgccgct aaacgccgtt
     7261 cttgcagcca agcaggctca gcgtgggctt gcgaaaaagc tactagtttt tcttttgtca
     7321 tctgtcaatc ctttgtctct ggtttttaaa ttggctgggt atatagaaca tcacctagct
     7381 ttttgtagaa agctaaagta attgtctcca aaaacttaac tttcttcctt atactcgatt
     7441 cccagatcct gtgcaatacc tgcgtagcct tctttttcaa gacgagtagc caaggcagca
     7501 tcaccagata agacaatacg accatccatc ataacatgaa ctaggtctgg ggtaatgtaa
     7561 ttcaacaaac gttgatagtg ggtgataatc atagctccaa agtctttccc acgcatctca
     7621 ttaacccctt tagagactac ttttagggca tcaatatcaa gaccagaatc aatttcatct
     7681 aacaaagcaa atttaggctc taacatgagc aattgaagaa tttcattgcg ttttttctcc
     7741 ccaccagaaa aaccttcatt gaggtaacgc tctgccatct cttctttcat acctaacaaa
     7801 gccattttct catcaagttt ggtgataaaa tctcgcacgg aaatcttatc cttatctgcc
     7861 ttaccggcat tcattgctgc acgcataaat tcagcgttgg taatccctgg aatttctgaa
     7921 gggtattgca tggcgagaaa aagtcccagt ctcgcacgct catcaacttc tagatcgaga
     7981 atattgaccc cgtcaagcaa aatttgccct tgggtcactt cgtagttagg atttcccata
     8041 atagctgctg aaagggtaga ctttcctgtc ccatttggcc ccataatggc agctacttcc
     8101 ccagttttaa gagttaggtt aacccctttc aaaatctctt taccttctat tgaaacatgt
     8161 aaattattaa tctcaagaat tgacatgata caccccttat tttctctgtc atctatacca
     8221 tctcaagaaa agacagtgtc atttgcactg ctttttttat aatggtcact gcatctatta
     8281 tagcaaaaaa agccttactc ggctttttta gtgtttcaaa tccttctttt ctttccattt
     8341 cagaagcatt gcttgacgat aatcactatt accaataaac tttaacaagt taaacaaagg
     8401 cgttcgcttt tcaccccaga tctccaatcc ttcaataaaa acttctaaac caaataaaag
     8461 tccaagcatc aagaggacgc caccaattcg actagaaaca tttaaaagta aagaaataag
     8521 ggaaaaaagc atggtaatac cataaactac taagacagct cctcgatgag taaagcccat
     8581 tgataagagt cgatgatgca ggtgcatctt atccggctcg tagaattttt gacctgataa
     8641 actacgtcga ataatagcca cgatggtatc cataatgggt acaccaagaa taataacagg
     8701 tgtcaccaca gccacagccg ttgaattttt caatccttga agagacaaaa cccctatcat
     8761 aaagccaata aagagggctc ctgtgtcacc caaataaatc atagctggat ggtaattata
     8821 aggaaaaaat cctgcaatag cagaaatcaa gaccaaaatg gttaacgtca aaaagaaatc
     8881 cttttgaggt aaaaagaaat aagatacaat agccatggtc actaaactaa taatagagac
     8941 cccactaacc aaaccatcca aaccgtcaat caagttaata gcattagtga tggaaacaat
     9001 ccacaggact gttaagaata aggttaagaa aggaccaaaa actaacaacg gccctccaaa
     9061 aggaattttg aagctatcaa atttaaagtc ggtaaaagcc cagacaatca ctgcaccaat
     9121 tagaatacca aacatcttta atttaggact taactcatac aaatcatcta aaaaaccagt
     9181 aagggtaatt actgtagcac caatcacgac aggtaaaata taactgaagt aggtctgacc
     9241 aaaaaacatg gcgcctttag aagcaatcgg aataagtccc aaacttgcta ctaaaaaaga
     9301 cataaaaata gctaatccac cactggttgg catcggaacc ttattgaccc gtctggcatt
     9361 gggattgtct actgcaccca cacgaaaggc taaaaagcga acaagagggg ttaagaataa
     9421 ggacattaac aaagccccta tcagaacaag gacataatct attgtgaatg aaaacatact
     9481 attttatccc aactcaatct tttgtaattg ctcaacagca ccatcaagca tcaattgtaa
     9541 gccatgctct tgtaaataag ctcttgtttt tgatccaggg tttgcatgct caatcaatct
     9601 cgcatacatg acattcgcaa aataagatgg ttgttgctga acatctaata aaatagtcat
     9661 atggtagcag ttgctcccct tatataactc tgaagcttct atcgaaaaat caatcgtttt
     9721 agcaaaagcc actgcctctg taatcgttga aaaatccaat acataatgga catagtctaa
     9781 aggttctgac tcatgattag ttgatggatc agccgcctca gactgattag ctagagtagc
     9841 ttcaacaaca tcttccatta tctcttcaat tttttccaat ttttcatgag ctttgacatc
     9901 tcccttttca cgcatggatt gctccaagct cttgaaaaaa tcttccggag tcatttggga
     9961 catatcacca aactctgcta aatcttcaag attaatatct ttatttatct cagatttggt
    10021 aacaaaaaca tctagacggt ccttgcgtgg agtcactcga aaactaagca taccactatc
    10081 tttaaaatta tctggaagat cgagttcgtc catcacagaa tagaaaaact cttctgtttt
    10141 ttcttgagga atcaagaaat ctttcaactc cattcctctt tcttctaaat cgtccatact
    10201 aattgttatt tttagcgttg tctcgctaat ttgtttcatt tccattctca atacctcgca
    10261 cgcgtagtca ttttattata ctagaaatgc tgtaatttcg caatttatga ctaactcctc
    10321 ttctccaaaa ggaaatttaa aaagcgacag tccccaaggt ctgtcgcaac atcctaaacg
    10381 atatgtaagt ttaaaaaaca aatttaaaga agctataaat aagtaaaagg cttcctaaca
    10441 caatacggta tttcccaaaa atggtgaaat cgtgtttctt aacataatct gtcaagaacc
    10501 taatcgccaa taaactaacc acaaaagcgg tcaaactagc cactaaaaga ataaggactt
    10561 gtgcaaaatc aaggtgatgg ccatcaagga aaaatttcac agcctttaat ccactatatc
    10621 caaacatggt aggaatggcc aaaaagaagg tgaaatcagc ggccactgtt cgactagttc
    10681 ctaaaataat agctcctaag atggtagccc ctgatcgact agtaccaggc acaatactta
    10741 agacttgaaa acaaccaatg aagaaagctg ttttgtaaga cattctagct aattcagtga
    10801 cggcaggttc ttgttgagca tttcgttttt caatccagat aaaggcaatc ccgtagacga
    10861 ttaaagcgat ggcaatcgga accataaaat aaaaatgtgc ttcaaaccaa ttatcaagag
    10921 gaaccgctat taaaatagaa ggaatacacg caatcaccac ttttagccat aattgccagg
    10981 taagctgtac ttctcttgct gttttcccag gttgaaaggg attgagacgt tcaaaataaa
    11041 tcaacataac agcgataatc gcccctaatt gaataacgat attaaacatt tctataaaag
    11101 ccttatcctg atttagtcgg ataaattcct gcaccaaaat gaggtgacca gtgcttgaaa
    11161 ctggtaacca ttctgtgatt ccctcaatga tgccaaagaa aatggctttt agcaattcga
    11221 taattaacat gttttctcct tattaaccac tcttattgtg attttctaaa ctgtgattaa
    11281 tgccaagcag cttttttaaa aggcaccgcc gccgcctcct ccgccaccgc cagagaagcc
    11341 gccaccacca ctaatcccgc ctccggaaga gactgagaaa tttgaggaag ttgtagctga
    11401 agacaagcta gacaaaaagg ttggtgtcgt agcatacatt accattgata attcacccgg
    11461 tcgaacagct tggtaaatct caggtaaggc aatacgatgg actttcaaat agcgttccac
    11521 tttcttagcg taaccaaata aggtggcata aaccaaaaca cggttccaaa caaccaaacc
    11581 ttcaatagca acatcctcaa atttgtcaat gtctcgtatc atgtttttaa agctctgcca
    11641 ttgatgtaac cgaacacccc cctcaggtgt ttcaataccc aatttcttgt attgatctaa
    11701 cttcaggcta atgtaaaagc caaggcacat cgttagcacc attaaagcaa ggtaaatgag
    11761 ggctaaccca ctttctttaa tagccaagta aatcaataaa ccactattta gaacaagcaa
    11821 caaacaacct aatccctgga cactattgct ttttcgtttc tctgccgggg tcatttgacg
    11881 ataagtatct ggtaagttta actttttgat agtttctagg acattgtttg taatagctgc
    11941 cgacgctttt ttcatcgcct taatcacctg ctcagacgat tgacggactt cttgctcgag
    12001 ctttttacct ttgtacgttt tctttaattg tttaatggta tcagcatcat aatggtattg
    12061 actaaacaat tgatccaccg gcaatgtcac cttgtttcca aaagccatat ctaagaaggc
    12121 agcttcctca tctgtcacac gatctaactg actgatttca agataagctt tcccttcttc
    12181 tttcgttaat aaaagcacct tttggtcgat cagatccaac aaggtagctt gaacaagggc
    12241 ctcaaaggtc actcctttag ggatcaataa tttctggctt tctgatgctg taggggataa
    12301 ataggcaaaa ctctgtccat aaatcgcttg tgtcaacaca agaggcgaaa gatcttcagg
    12361 gacttcgtaa ctgtgatctg tatggttggc caaatgataa cgattgaatt gtttctttaa
    12421 tcttataaac tgtattaact gccacagaag aaaacctacc tctaccagag gaatgacttt
    12481 gccaaatagc agctgtaaca aggcccttcg tctggaaatc ttagtttcca acgctacaat
    12541 tttattcttt ttactcgttg aaacatcaac tggttcttct cccaataatg cttttttatc
    12601 ccaataagca tgcaattcca attgccctga gacatttttg gcattaatct gatagcgact
    12661 gttaccttct tttaacactt gaggtttctt ttggtaatat cctctgtgag cccatagatt
    12721 agaatcctga atatcagttg gggtagtgac ggtcaaactc accttttcta atgttttgtc
    12781 ccaatcacta atcggtgtcc aattcagctc tgccacgtct tgataagctg ttaaaaggtg
    12841 gtgcagttgc cagataacct taacgtcaac cttatcacca gcttggccag cattataaag
    12901 ttttaaacga tagccatctc caaggtcact aaactcttga ctaacaggta cctgttgacc
    12961 attctgatat acctcaactt taggcttttg gtcaatagca aagcccgctg gcaaatgacc
    13021 tgcccggccc agcgaaatat actggccgtt ataagaagtg tcaaattgat aggttacttt
    13081 ttgctcaaac cgagcagtat tttcttttga taacagtaac tgaccttcat agttggtgat
    13141 gctatagtct acatcagctg cttttattcg tatccctaaa agactgaagc agagcacaag
    13201 cgtcatcaaa atttttttca tggcaacatc ctctttctgc cctcatttta ccatatttta
    13261 aggagatgac aaaacctaaa ccaagtcctt catcttattt ttaatcatta ttttgaatat
    13321 ttttccatat ttcaacctat tcactgataa tatattgaaa taggatgcaa attgctgtaa
    13381 aataggtctg aatactattt ttgaaacagg agacctatat gaaaaagcta atccttagct
    13441 gtttggtcgc cttggccctt ctgtttggag gaatgagccg ggctcaagca aaccaatatt
    13501 taagagtcgg aatggaagca gcctatgctc cttttaactg gactcaagat gacgcttcaa
    13561 acggggctgt ccctattgag ggaactagcc aatacgccaa cggttacgat gtccaagtcg
    13621 ctaaaaaagt cgctaaagct atgaacaaag aactgttagt cgttaagacc tcttggaccg
    13681 gtttaattcc agcattaact tctggaaaaa tcgatatgat cgctgctggt atgagtccta
    13741 ccaaagagcg tagaaacgaa attagcttct caaacagcta ctacactagc caacctgttc
    13801 tagtcgtaac tgctaatggc aaatatgctg atgcaacaag cctcaaggat ttttctggag
    13861 ctaaagtaac tgcccagcaa ggcgtttggc atgtcaatct cttaactcaa ctaaaaggtg
    13921 ctaagttaca aacaccaatg ggagatttct ctcaaatgcg acaagccctt acttcgggtg
    13981 ttatcgatgc ctatatttct gaacggcctg aagccatgac tgctgaagct gctgatagcc
    14041 gtttgaaaat gatcactctt aaaaaagggt ttgctgttgc tgaatcagat gctgctatcg
    14101 ctgtcggaat gaaaaaaaat gacgatcgta tggcaactgt caaccaagtg cttgaaggat
    14161 tttctcaaac ggatcgtatg gccctgatgg atgatatggt taccaaacaa cccgtggaaa
    14221 agaaagccga agatgctaaa gcatcatttc taggccaaat gtgggctatt ttaaaaggta
    14281 actggaagca attcttacgt ggaactggaa tgacccttct gatttccatg gtcggaacca
    14341 ttacaggtct ctttattgga ttattaatcg gtattttccg tacagctcct aaagctaagc
    14401 ataaagtagc tgccttggga caaaaactct ttggttggtt actcactatt tacatcgaaa
    14461 tcttccgtgg gacacctatg attgttcaat ctatggttat ttactacgga acagcgcaag
    14521 cctttggtat ttcgatcgac cgtaccctag cggctatttt tatcgtatct atcaatacgg
    14581 gtgcctatat gagtgaaatt gttcgcggtg gtattttcgc tgtcgacaaa ggtcaattcg
    14641 aagcagcaac tgctcttggc tttactcacg gacaaaccat gcgtaaaatc gtgctaccac
    14701 aagttgttcg caacatttta ccagcaacag gtaatgagtt tgtcatcaat atcaaagata
    14761 cttctgtttt gaatgttatc tctgttgtgg aactttactt ctcaggtaat accgtagcca
    14821 cacaaaccta ccaatatttc caaaccttta cgattatcgc cattatctac tttgttctta
    14881 ccttcacggt gacacgtatt cttcgtcata ttgaacgccg tttcgatgcc gatacttaca
    14941 ccactggagc aaaccaaatg cagattgcgg aggtctcaaa tgtctaactc aattattgaa
    15001 atcaaaaacc tcaaaaaatc ttacggcagc aatgaagtac tcaaagatat ctccctttct
    15061 gttaacaagg gagaagtgat ttccattatc ggttcttctg gttctggaaa atcaaccctc
    15121 ctgcgctcga tcaatctttt ggaagagcca agtgccggtc aaatcctctt tcatggtgaa
    15181 gatgtccttg ctgaacacta caacttgact cactaccgtg aaaaactagg catggttttc
    15241 caatccttta acttgtttga aaacctaaat gtattggaaa atgctatcgt cgctcaaacc
    15301 accgtcctca aaagagaccg tgcacaagcc gaacaaatcg ctaaagaaaa cctcaatgcg
    15361 gttggtatga cagaacaata ctggcaagcc aaaccaaaac aattatcagg ggggcaaaaa
    15421 caacgggttg ccattgccag agccttatct gtcaatccag aagccatgct ctttgatgaa
    15481 ccaacatcag ctcttgatcc tgaaatggtt ggtgaggtcc ttaaaaccat gcaagacttg
    15541 gcaaaatcag ggttgaccat gattattgtt acccatgaaa tggaatttgc tcgtgatgtt
    15601 tctgaccata tcatcttcat ggataaggga ttgattactg aagaaggaag tcctcaacaa
    15661 attttcgaga atcctacaca agatcgtacc aaagaatttt tacaacgctt cttaaaataa
    15721 gataaaggat ccaactattt ctatgccagt tgggtctttt ttcaattcaa aaagaatttg
    15781 tcataaaata ctttaccagt taactatttt atgctatact tatttttgtt aacgttaata
    15841 atttatattt aggaggataa tatggcagaa aaaactgact tatcgtcagc ttacagaaga
    15901 ctgaaaagtc ccaatattaa aaccagaaaa cgtgctctga agattattca cgagtacaaa
    15961 cgttacggta aaaaataaga ttttactttt tatctctcca aatttaagtc agacaaaaaa
    16021 aggctaagac accccttcca tcttagcctt tttgtataca gtttattaag ggaactattt
    16081 taaaatatct tttatgctag agtctagaat agttcaaaac gttaaaatca tacatttaag
    16141 gtagtgttta aagtatacga aaaagaactc agcataaacc aagctctctt tcgttataac
    16201 taattattta gcaatttttg cgaagtactc aagagtacgt acaagttgag cagtgtaaga
    16261 catttcgttg tcataccatg atacaacttt aaccaattgt gatccgtcaa cttccattac
    16321 tttagtttga gttgcgtcaa acaatgaacc gtatgatacg cctacgatat ctgaagaaac
    16381 gattggatct tcagtgtaac cgaagctatc gtttgaagca gctttcatag cagcgttgat
    16441 ttcgtcaaca gaaacgtttt tgtcaagagt tacaaccaac tcagttactg atccagttgg
    16501 aacaggaaca cgttgtgcag caccgtcaag tttaccgtta agttctggga taacaagacc
    16561 gatagcttta gcagcaccag ttgagttagg aacgatattt gcagcaccag cgcgtgcacg
    16621 acgaaggtca ccaccacggt gtggtccgtc aaggatcatt tggtcaccag tgtaagcgtg
    16681 gattgtagtc ataagacctt tttgaatacc gaatgcatcg tgaagagctt tagccatagg
    16741 agctaaacag tttgtagtac atgaagcacc tgagataact gtttcagtac cgtcaagaat
    16801 gtcgtggtta gtgttgaaaa caactgtttt aacatcgttt ccaccaggag ctgtgataac
    16861 aactttttta gcaccgttag catgtaagtg tttttcagct gcttcttttt tagcaaagaa
    16921 accagttgct tcaagaacga tttcaacccc atcagttgcc cagtcgatgt tttctggatc
    16981 acgttcagca gaaactttga tgaagtttcc gtttacttca aatccacctt ctttaacttc
    17041 aactgttcca tcaaaacgac cttgagttgt atcgtatttc aacaagtgtg caagcatatt
    17101 tggatctgta aggtcattga tacgagttac ttcaacacct tcgatgtttt gaatacggcg
    17161 gaatgcaaga cgtccgatac gaccgaaacc attaatacca actttaacta ccattagtga
    17221 tttcctcctt atgaaaatca aaagaatatt atttagaggg aatgctccct acctattgtg
    17281 aaaagattaa cttacagtca tctgcaacaa cctttcaaca gaactattat ataactattt
    17341 cagaaaaaat gcaaataaat tagccatttt cataaagggc agcttctttc ttctagcctt
    17401 caaaacccac caaatatagg aaagggcttt cttttttcta tgttctattc tgtaatttat
    17461 ctttttattg ggaaagattt ccaaaacttc aaactgtact ataacgttct gttacactaa
    17521 aaaagaggtt aggatagtta tcctcaacct cttagtggtg gttattcacc tttgtttttc
    17581 ttaatgattt cttcttgaac tgacttagga acatcttcat agtggtcaaa taccatcatg
    17641 aatgtaccac gtccttgagt agcagaacga agaactgttg cataaccgaa catttcagca
    17701 agtggaacat aagcacgaac gatttggctg ttaccatgtg cttccatacc gtcaacgcgt
    17761 ccacgacgag ctgtcacgtg tcccataacg tctccaagat tgtcttctgg agctgtaatc
    17821 gttacaagca tcatcggctc aaggatagct ggttgtgcag atttagctgc ttctttaagt
    17881 gcaagtgatg cagcgatctt gaaggcagtt tcagatgagt cgacatcgtg gtatgaacca
    17941 tcgtaaagct tagctttaac atcaaccatt gggtaacctg caagaacacc gtttgccata
    18001 gattcgatca accctttttc aacagctggg ataaattcac gaggaactac accaccaacg
    18061 atagcatttt cgaattcgaa acctttacct tcttcatttg gtgtaaattc aatccaaaca
    18121 tcaccgaatt gacctttacc accagattgg cgtttgaaga atccacgagc ttgtgtagaa
    18181 gcacggaatg tttcacggta agatacttga ggagcaccaa cgttagcttc aactttgaac
    18241 tcacgtttca tacggtcaac aaggacatca aggtgaagtt cacccatacc agcgataact
    18301 gtttcaccag tttcaacgtt tgtttcaacg cggaatgttg gatcttcttc agcaagtttt
    18361 tgaagggcaa cacccatctt gtcttggtct gctttagatt ttggctcaac catcaactgg
    18421 ataactggtt ctggaacttc gattgactca agaataactt ttgctttttc atcagtcaat
    18481 gagtcaccag ttgttgtgtc tttcaaacca acagcggcag cgatgtcacc agcgtaaact
    18541 gtttcaattt cttgacggct gtttgcgtgc atttgaagga tacgtccgat acgctcacgt
    18601 ttaccttttg atgtgttcat aacgtaagaa ccgctattaa gaacacctga gtaaacacgg
    18661 aagaatgtca aacgacctac gaatgggtca gtcatgatct tgaaggcaag agctgcaaat
    18721 ggctcttcat cagatgctgg acgttcttct tcagcatctg tatccgggtt aacacctttg
    18781 attgcaggga tgtcaagtgg gcttggaagg tatgcgataa ccgcatcaag catcaattga
    18841 acacctttgt ttttgaaggc agaaccacaa agtactggga agaattcaac gttgattgtt
    18901 gctttacgga taccagcaat caattcgtcg ttagtgattt cttcaccttc aaggtatttc
    18961 atcatcaagt cttcatcagt ttcagcaact gcttcgatta atttttcacg gtattcttga
    19021 gcttgttcta agtattcttc tggaatatct tcttcaagga tatctgtacc aaggtcgttg
    19081 gtatagattt ctgctttcat cttgatcaag tcaatgatac cacggaaatc atcttctgca
    19141 ccgattggca attggattgg gtgtgcattt gcttgaagac ggtcatgaag ggtttgtact
    19201 gagtaaagga agtcagcacc gattttatcc attttgttgg cgaaaacgat acgtggaaca
    19261 ccgtattcag ttgcttgacg ccaaactgtt tcagtttgag gctcaacacc tgattgtgag
    19321 tcaagaacgg ttacagcacc atcaagaaca cggagagaac gttgtacttc gatggtgaag
    19381 tccacgtgtc ctggtgtgtc gatgatgtta acgcggtgac catcccattg agctgttgta
    19441 gcggcagaag taatagtgat accacgttct tgctcttgct ccatccagtc catttgtgat
    19501 gcgccttcgt gtgtttcacc gattttatga attttaccag tgtagtaaag gatacgctct
    19561 gttgtagttg ttttaccagc atcgacgtga gccatgatac cgatattacg agtttttgca
    19621 agtgaaaatt cgcgagccat gtgtttctcc tattttttta gtttactctt tcattatagc
    19681 actatttagc aaaaacggat aggcaggacc tacccgttat ctaaatattt gttggtaaag
    19741 ataataccta ttgcctttat aaggcttggt atcttattca ttgaactcag gctaaacgct
    19801 gtgtgaaaaa gataaacttt cctagagaca aaggtttctt cgtcaaattt cctattttca
    19861 ctttgcgttc tatacgcctt cgtatcttat taattgagtt cgagctacaa gccactgaca
    19921 aaaagataat agttcctaga aactgaagtt tctgcgtcaa tttccctatt ttgtctagca
    19981 tttgtttgac gctaaaatat cttatattac caacggaagt gagcaaaggc acggttagct
    20041 tcagccattt tgtgagtgtc ttcacgtttc ttaactgatg cacctgtgtt gtttgcagca
    20101 tccatgattt ctttagcaag acgatctttc atagtgtgtt caccacgagc acgtgatgcg
    20161 tttaccaacc aacgaagtcc aagtgttgta cgacgttctg gacgaacttc aactgggact
    20221 tggtagttag aaccaccgac acggcgtgcg cgtacttcaa gtacaggcat gatgttgtcc
    20281 atagctgttt caaatacttc aagagcgtca tttcctgttg cttctttgat agcgctgaaa
    20341 gcatcgtaaa cgatagtagc agctgtacca cgtttaccgt caagcataac acggttgata
    20401 agacgtgtta cgatttttga gttatataat ggatctggta atacttcgcg tttaggcgct
    20461 tgatttttac gactcatttt ctcttatccc ccttctatta tccttttgga cgtttcgcac
    20521 cgtatttaga acggccttgt ttacggtcag caacacctgc agtatcaagt gcgccacgaa
    20581 cgatatggta acgtacccct ggaaggtctt ttacacgtcc gccacggata agaacaacgc
    20641 tgtgctcttg caagttgtgt ccgatacctg ggatgtaggc agttacttcg ataaggttgc
    20701 tcaaacgtac acgagcgaat ttacgaaggg ctgagttagg ttttttaggt gtcattgttc
    20761 caacacgagt agcaacgcca cgtttttgtg gggcagccat tttagtttga acttttttgt
    20821 gactgttgta accaatgttc aaagctggtg aatctgattt ttcaatttta gatttacgtg
    20881 gtttacgtac caactggtta attgtaggca tctacattct cctgtgtttt tttatttttg
    20941 gttgatatag cacttggtga cagcccatat ccggtgcact ttttgcaaca attgtcagca
    21001 cgtctctgta cacttttgag agaccaaaag taaaaagtac cgtctaatat aataacacag
    21061 acggtacttc atgtcaaata gaatttttcc tttgatgcaa ttgcttatga cgaatgcatc
    21121 acttagattc cttacggaac ctaaatcctg tgaggccgac agctgccaac atgacgactc
    21181 ctgctttaag agctctttga gtactttcat caacatcaga aatgagattg ctcgttgtag
    21241 aagatcctga gccataagat gtcttagatg agggctgagt aatcgaagat gtgattttag
    21301 acacaatggc attacttgct accatttcag aggtttggcc aacaagcgct tctgctacaa
    21361 gacttgtatt ttcagcagct aatcttgctg aagcttctaa tagttgtttc gtttctttaa
    21421 ccatttcttg aacaagcgga gtagtatcta tcttactata tgatagcggt tttacgcccg
    21481 taagaggtgg agctacttgc aaatcaggca cagtagctat atcttcgtct aaatggcgat
    21541 atcccttttc attagctaag gttttaagca aagtcaactg gtgttctgtg gtagcaatag
    21601 tagcttctag actgctttgt ttagcttgta aggctgctaa agtttcttgt gcatttaaca
    21661 aagatgaggt agttttagct aaatcttgct tagtattatc aatgcgctca cgtatcactt
    21721 gaaggcggtt aggattcaat ttaaagtccc ttagatattg caaatgagct tttttagcca
    21781 ccagtgctgt cactctggct gcggcttgct ctgctaagac ttctgtctgg tgcagtgcgg
    21841 ctttcaacga tgctagctta gctaatgatt gatcacgagt agcttcgagt tgtgcttgtt
    21901 ttgctttagc tgctagtaat tctgttctca aagaaccttt agtatcattt aattgtctca
    21961 cttggaggtt taagctgtct gactgcttaa gtgtgcttgc taatttacct tgaagttgac
    22021 ttactttagc ttgggctgcc ataatgtcag cttcttgatg aatagccgac aaacgatttt
    22081 ctaatgagct tacttttcct ttgatcgctg caatagtatc agatacagtg cctactcttt
    22141 gggcataatc ttttgtactt ccaacggctt ttataggggt cttattaaag cgttgatggt
    22201 tagcaatgtt agactctgga aacattacaa agtgttcatt caaagatcct acattgctgg
    22261 ttgaaaatcc aaggtaaaca ggcgcattag ggttacgttt atctacacgt aaaaagttaa
    22321 tagcatggcc gtatgtattt ccgtgtaaat gatctgtaaa gagcatatac ttgatactgt
    22381 cataaatacc acgtttaata ccattcacag tatgcacatc gttaaaagca ccgatattct
    22441 cgtacatgtt atcatcattt cgaatgagcc ctgacgctcc ggcagagtct tcaataatag
    22501 ttttatcatg aggcccaaca ccataatgcc ctgatacccc tggctgtccg tagacaaatg
    22561 atggtcttgt attaccatga gttttcttat agctggtact aagtaatctt gcaaattctt
    22621 gtgatcctgc tgtaacagta actggtggta gacctaattg tcttcttaca ctattaatca
    22681 tgtgagctgc aaactgcgct agctcatttt gcacttctgg tgtcaaatta tcgggatcaa
    22741 caaagcgatt acgatctgct ggaatatctt ggtattgatt taattgatta cctggactag
    22801 ctttggcaat aatttgatct gcatgctctt tgtaataatt attgtaacta gctgatccaa
    22861 tataaccact agcttctaat tttttaagtt cttcaagagg atagccttgc ggtactttca
    22921 tggtattatt acccacaatg ctatcttgag tagacggagc tgaggattta agacgactaa
    22981 gttctgcctc tttttctgct agagcagctt tctgagctgc caactcttca gtcaattgct
    23041 ttttaacttt agctttttga ttttctaagt cagctttagc cttctccaat tctgagctta
    23101 atgcttttgt attatcatta gccgtttgag ctgctttagt gatagcatca ggattgctaa
    23161 tcatagcatt gagcttagca atattttgtt cagacgtttt gacttgttcc actaaatctt
    23221 gagctcgagt agtttctgct gaaatgctag ctttttgttc tgacaatgca gtctcttttg
    23281 aatgttgatc tgcttgagca ttatgaagct ctgtttcagt agctgttaac tctctttgat
    23341 gttcggctcc ttgggctaat agcgtctcct cactacttgc aagagtatta gtgtaaattt
    23401 cttgtgcaga ggttaaagct ttttgttcat tatcttgctg ctcttttaag tggttgattt
    23461 cagcagtagt ttttgtcaga gcggtagcaa gctctgtcag ttctgctttt tgttgactga
    23521 gagttttttc aactgcatca atagttgcct ttgcctcttg aattgtttct ggttttggta
    23581 aactatcgtc gtgagtatta ctcgccttcg tttctcctga ggctctatca tctgctttga
    23641 cttggtgaga tacgcctaca ctggcactca ataaagcaat tgttgaggtt aaagcaattt
    23701 tctgcttaac ttggtttggc ttcgtttgtt ctaagtccat tctctctcct ttttccattt
    23761 atcgaaaata ttaccaactt ctacaacaac attgttgttt ttgacatcaa tctctgaaac
    23821 tttcagcaat gatttgaagg tcatttgctc acgctctgat tgagcgtttt ctgcaaacac
    23881 agcaacacca actaaagtgc tgtcaaattc tgtcaagaga ctgatcattc ctgtaatcgt
    23941 ccccccgccc ttcaagaaat catctacaat caatacacga ctattgggtt tcaaactacg
    24001 ctttgacaga aacatctttt caatacgatc actagaagca ctcgcataat tgacagacac
    24061 tgtcgatcct tctgttattt ttaaatcacg tctaacaatg acaaaaggaa cacttaaaat
    24121 attagccact gcatttgcta aaggaactcc tttggttgca actgtcatca cagcgtcaat
    24181 cttttctcct ttaaaggcat tggcaataat acgaccaata ttttgtaaaa ttttaggtgt
    24241 actcagcaag tctgacaaat agatatagcc tccaggtaag atgcgatcac tctctgataa
    24301 cctttgacac aggtcttcaa caatggtacg cgcttctgtt tctgaaatac tgggggtaaa
    24361 aattacgcct ccacttgctc ctgtaagtgt atcgatatca ccaatgttag cttcttcaaa
    24421 agctttcttg ataatagcga tatcttctga gatagaagat tttgcggcct catatttagt
    24481 tgcaaaggtg tttaaactag ttaatttata cggattgtta atcaaataat ttgaaatgac
    24541 aaccatacgt tcacttcgtc ttaatttcat agtttcccca tttttatgta ctttttgcta
    24601 ttataccata aagttaataa aaaacgaaca ttcgattagg aaaatattgg tttttttgtt
    24661 cttattaata tcatgcaaga attcaatcta agataaacta atcaacattc ctactgaaag
    24721 gacattctac ttaataacca taacaagagc caaaaacact tatgaaacca tcataagtgt
    24781 taagcacatc aaaaatcatc acaaaagcaa agtaactatt acttgagaaa cactgatcaa
    24841 tagtttggct tgtaaaaaga ccgattatcc atggcaaaaa tccgattggt catctcgccc
    24901 tcgctaacac ggctaagagc tgttgtcatc atcatcatat tagcatcaat attgtcaatc
    24961 atatgaataa tttcagcttc catgatgcgt ggacgtaccg gactaccgta ttctaattgc
    25021 ccgtgatggc ttaaaatcac gtgcctaagg acaatgactt cttctttggt atcatcgatt
    25081 tgtagttctg aaattacttt tgtaatttct tcgttaatga gagaaatatg cccaatcaag
    25141 ttaccacgaa cggtgtattc tgtattatct ggtcctgtta attctatgac tttagctaag
    25201 tcatgtaaca taatacctgc aaacagtaga ctcttattta agtcagggta gatatctcca
    25261 atgctatctg ctagtctgac catcgtagca gtatggtagg cgaggcctga ctcaaaagca
    25321 tggtggtttg ttttagctgc aggataagta taaaattctt tatcatactt acgataaaga
    25381 gcccttacaa ttcgttgcca cgttgcattt tcaattttaa agagcatctg ttccaaatag
    25441 tcacgtactt ctgtcacact gacaggtgcc ttttctttga agtcctttgg atcattgggc
    25501 tcacctgggc gaacatttcg caaggtgatt tgattaacct gaggggtacc attatacacc
    25561 tcgcgtcgcc ctttcataaa cacgacttta ccagcagtaa attcttcaac gttataaggt
    25621 tgggcatccc acagatttcc tgaaatctcg ccagtgtcat cctgaaaggt aaggctaata
    25681 aaatctttac cagctcttgt cttacgaacc tctgccgact taatgaggta gaacccttca
    25741 aaaagctggt cttttttcat ttgattaatt ttcattattt tcctcttcta ataagggcat
    25801 gttcaagaga gattttgttg tttggtcttg ataagtttca acggtattca aggctcgaac
    25861 aatggcattt gtccttgttg aaatgagctg atccagcgta ttattagctg tattcatttg
    25921 cttttgagcc ttggcaagca ggccaccaaa tttatcgaat tctaacttga cattgcccaa
    25981 aattttactg atgtcatcag catttttttg gatattaagg gtcttgaaac caacagataa
    26041 ggaattgagc aaagcagaca gggtcgaagg acctgcaacc acaatatttt cttcccgacg
    26101 aaggctatca aagaaagccg catttctgac cacttctgaa taaagacctt ctgttggtaa
    26161 gaacataact ccgaaattgg tcgtctctgg ggggttcaag tactttttat gaatgtcttt
    26221 ggcaaagcgt ttgatagctg ccagaagtgc ttttcggcta gcctcgatgg ccagtttatc
    26281 accaacttcg taagcatctt ctaatcggta ataatcttca agagggaatt ttgagtcaat
    26341 cggtaggtaa atataaccgc cttgaccatt tcctgggagc ttaatcgcat attctacgcg
    26401 ttcactagaa ccactaaccg ttacaaattc tctttcgtac tggcttgatg tcatgatatc
    26461 ctcaatgatt tggcctagtt gaagttcgcc taaaatgcct cgtgttttgg tattggacaa
    26521 aaccttattt aaagtaccca catcttgagc cacgctacgc atttctccca agcctttatt
    26581 gacactttct agttgcttgg atacagaatc gaaagaggcg tgcagacgat tttttaaggt
    26641 ttcttccaat ttttcttcaa cgatctggcg catttcctcc aaacgttttt cgttagattc
    26701 ttgcatattt ttgagcgatt ggttgacctg ctggttaatt tcttctaagc gtttgtcaga
    26761 ccggtcccta ctatcagaca aactacggtg caagacgtca cgaatatctg ttaattgttg
    26821 gtaaaggtct gcttgttgtc ggttcatcag ctgtgttagc tctaacaatt gttgcttgtt
    26881 agctgtatcc aactggtagg ttatttggtc agacaaatta tccgcgttgc cttctagggt
    26941 ttgggcaagc tgatgttgaa ggccgttgac tttgaacaac agataagccc ctaaacctaa
    27001 gagaaccaag accaaaagga acaagataag gtccatacta actccaatct ttactttgaa
    27061 taataatcaa gtagccactt ggcaaactaa ctgtaatagg ttgatggata aaggcattac
    27121 tggaatagat tttctttttg aaaaaattat cttgcgtcaa ctcaaattta gcaccagtaa
    27181 tggtcaagtc agcttctcct tctgccataa atgccacata ggtcatccct tcttcttgat
    27241 ggataaggtg ctgacctgcc gggcggtagg taatcatatt ttgttggtca cgcaaggcga
    27301 tttgtgccat aaagggagca ataccagggt cgctaggcaa gaaaatattg gataagaggt
    27361 ggtccattcg acccccaaaa gccccaaaaa tggtcacttc cgcctcagga aaacgagcaa
    27421 agacctcttt aagggctaat tccgtatccg tatcattttt ttctggatga gcagtaataa
    27481 aaagctctgc cttctctttt atatccgtaa acgctttttg ggaaaccgaa tcaaaatcac
    27541 caacagccat attcaaagga agcccatttt ctaataaaaa taggctccct ctatcaatac
    27601 cgacaaaata atcaaaatca cggctgatat aagagagatc tcctcccgca aaaagagcaa
    27661 ctttagacat tcaaggccgt ccttaaggtt tggacttggc tcactaggtc actggccttg
    27721 aaaaggtaag aacctgccac aaagacattg gcccctgctt cataacaagc acgaatggtc
    27781 ttattgtcta ctccgccatc aacctcaatg tcaaaggaca atcctttttc atctcgccat
    27841 ttggctacgg tagccacttt ttctaaacat tctgggataa aggcctgacc cccaaaacct
    27901 gggttgactg tcataatgag gacttggtca actaagtcta gcaaaggttc tagggctgtt
    27961 gcaggagttc ctggattaat cactacccct gctttcatgc cagctgcctt gattttttga
    28021 agggctccat gaatatgacg agtactttct gtgtgaatgg tcatgatatc aacaccagct
    28081 tgagcaaaag cctcaacata gcgttcagga tcaaccacca ttagatggca atcaaagacc
    28141 aatttactgt gctttctcat acttgccaca acatctgcac caaaactgat gttaggaaca
    28201 aattgaccat ccataatatc aatgtgaacg tattcggcat ctgtttcttc aatacgagct
    28261 agctcagaag caaaattagc ataatcagct gctagaatag atggggcaat ttttagagtt
    28321 gacatggagt agacctactt tcttttgata acttttttat atgtttcacg acggttttca
    28381 atttcactga gaaattgcag gtaatgctca taacgaactg gccacaactc tcccgtttca
    28441 agcgctgcct tgacagcaca cttgggttcg tgggtatggg tacaggatct aaacttacac
    28501 tcatggctca gccggcgcag ctctggaaaa gcctcgttca aatcttcagc attagcaata
    28561 tcatagtcta atgatgaaaa acctggcgta tccgcgattt ttccgccatg ggtattgtaa
    28621 aaactcacag ctcgggtggt gtgacgacca cgccctagac tatctgagat ttctcctgtt
    28681 tcaagagcca attcgggggc gattcggttg agaagggttg atttcccaac acctgtttgt
    28741 cccataaaga cggtaatttt atcagctagc aaaggcaaca attcctcaag actggtcaca
    28801 aaatcgtaac cgattgcctg atactgtcga ccaatagcct taatctcctc gggactatct
    28861 aataagtcca tcttagagat ataaaccact ggatgaatgg ctttgtgttc aagcaagatg
    28921 agaaagcggt ctagaagatt gctgttaaat tctggttctt tagcagacat aataaccact
    28981 gcttggtcaa tattgacgat aggaggtctt accaaactgt ttttacgagg atgaatcgct
    29041 aaaatataac cttcagagtt gtcctctgct gagaaatcca cgatatctcc aacataaggt
    29101 gtctcaccgc gcttcctaaa attaccacgc gctcgcgtct gatatacctg tccttctgac
    29161 tccacgtaat aaaaaccagc cagagattta atgattttac cttgcaactg tgctcctttg
    29221 ccctactata ataactctat tatatcagaa aaccgctccc aaatcagcat ttgactctaa
    29281 gtttcactcg ccttaaaaaa ctgtcaagat tctctagacc actggctatc atttaggtca
    29341 ctgcttttta aaaatagaat attttatcaa ctaaaaatca gatcactgat gatttttgag
    29401 gtaggaaacg atatgaattt taagaagacc tttgctattt acgaaaaaga gtctaaccgt
    29461 accgctatag tcacgctctt gttaattgcc ccttttttcc ggggtattaa aaaaactagc
    29521 tctgcactgc gataccttaa cggccagctt caataagaaa aatccccctg aactgaacat
    29581 ttctgttttg tctacaaggg aattttttta ttgtcaccgg gagaaagctt ttataatcca
    29641 acttctttta aagcatcagc taatttgcca aaatcttgaa tcgacaaggc ctcaccacga
    29701 atagaaggct tgatatctgc tagtgctaga cctttttcaa gtttagtctt gatgtcttca
    29761 gacttcccaa aatggctagt caagttattc cacagggtct tgcgacgatg gacaaaactg
    29821 agacgagata ctctgaaaaa gaaatcttcg tccttaacct caatcaaagg ctggtcgcga
    29881 cgcaccattt tgaggatagc actatcgaca tttggtgcag gtacaaagac ggtgcgcggc
    29941 acaatgaagg caaccttggc agtcatgtaa tactgcaccg caatggacaa ggaaccataa
    30001 gctttagtgt ttggctcagc cgagatacga tctgccactt cacgctgcat catgactaca
    30061 aactcttgga atggaatttt gctctcaatc aagtgcatga gaataggcgt tgtgatgtag
    30121 taaggtagat tagccaccac cttaatgggc aaatcaggat ttttaaactg cttgatttgg
    30181 gtctgcaaat cagcttttaa aatatcttga ttgaccactt gcacattatc aaaatcgcgc
    30241 aaggtatctg ccaaaatcgg caccaagcgg tcatcaatct caaaagccat cacttcagca
    30301 gcattttctg ccaaaaattc cgtcaaagcc ccaattccag gaccaatctc gataacattg
    30361 acattctgat caatctcagc cgtgtcaacg attttctgta aaatattggt atcggtcaaa
    30421 aagttctgac caaaagattt cttaaaggta aatccgtgac gatcaaggac agccttggtc
    30481 acgctataat ctgcaattct catatattct cttcttttta tgatcacttt ctttttggca
    30541 tttgaaccac tagcctaaac ttttttgcca agatacgcca ttcagttaat ggggctgtta
    30601 tattttattg tactctttac tcctcatatg tctccatcac ttcttccact tctgctaggg
    30661 taatgccaaa taattcgagg cgcttgagca gttgtttgcc gttggcatag ccgatgcgga
    30721 gcttttcgcc caagtattcc ctgcgtttac ggctatctga cgccattaaa agccctagac
    30781 gaatcaggtc tgtttgacga atgtcaaaat aagactcatc atcatattgc tgagtgacat
    30841 gagcaagtgc cttttggagg tcctcaaaat tggcatgttc aacccctaag gaacggccct
    30901 tactcttaga actcggaacc gcctcgtttc ggtttaaaaa ggcgtgacga gctgttggca
    30961 cagctgccat aatgagctta cgaatgcgct caccattata atctgggtct gtcaaaacaa
    31021 tgacaccacg caagtcattt aggcggttaa tccgttctaa atcttcttca gtgatagcag
    31081 agcctctggt ctcataggta tccacctcgt aaaaacggcg aaggttagcc gtgtcgtcct
    31141 tgccttctac aacaagaacc tcttgaatat taattttttc agtcaagctt aagtaccctc
    31201 tttgcattag cagtcgtttc ttttgcaact tcttccacgg tcattccccg tagctctgcg
    31261 attttatcaa ccacataacg ggtataagct gtatgatttt gtttgccacg ttttggaact
    31321 ggtgtaagat aaggggcatc tgtttccacc aaaatcttat ccaaaggcaa gtgctgcgct
    31381 gcctcttgaa tatcaagggc ctttttgaaa gtgaccacac ctgaaaaaga aatcatcatg
    31441 ccaagttcaa tgaaacgttc agccatctct aatgatcctg agtaagaatg catgatgcct
    31501 ccacgagggc caacaccagc tgctttgatg acctcatagg tatcttccaa ggcatcgcgg
    31561 gtgtgcacca caaaaggcaa atcatggtct tttgctaatt gcatttggcg cttaaaaacc
    31621 tctatttgca cctctttggg gtcctccatc caataatagt ctaagccaat ttcacctaag
    31681 gcaatgactt taggatgtga taattgagag acgatagact cctcaactgc ttcagaataa
    31741 gatcctgctt ctgtcgggtg ccatccaata gtagcataca cgttaggata ctggtctgcg
    31801 agagcaagtg ctccactaat agtggcctgg tcaaagccaa ccacattatg gtaggccacg
    31861 cccatttctt gagccaaagt caattcttca gtttcgtgtc cttgaaattc tgctacattc
    31921 aggtgcgtat gggtatcaaa aatagttagt ttttctactt tatttgtcat gatgttatta
    31981 taacataaag atcttaacca catcagaaaa cttggtgaga acttctgccg tttaggttga
    32041 tcgtaaaacc ctttgctagc tgacacttgt ctattttcta cttttttgac aaatgacctt
    32101 taatagtcgg ctcctacata agccctcaaa aatactacaa cctactcaaa taaagtgggt
    32161 tgaacaaatc tgtatcacaa aaagggtcgc taccttctgt tagcgatcta tgctatacta
    32221 gtagatagtt aaactgtaag gagtatgctt tgctatgcct aaaaaccacc gcctaattac
    32281 caaaatcgaa gcatcattgg aacatatgac cagtttagaa aaagggattg ctcatttttt
    32341 tattacaacc gatctgacac ctcaagagtt gaccgcatcc gagatagtta agcgactaca
    32401 catctctcaa gccgccttga ctcgatttgc aaaaaagtgt ggttttactg gttatcgtgc
    32461 ttttgccttt gattaccttc acagtcttca agaatcacaa gagactttcc aatctattca
    32521 cttagaattg acaaagcgcg tgctgatgga ctacgatgct cttattaata aaacctatga
    32581 gctcgttaat gaagaaaaat tactgaactt agccaagctg atcgatgctt ctgagcgagt
    32641 ctatttcttt ggcaaaggat cttctggact agtagcaaga gaaatgaagc tacgctttat
    32701 gcgacttggt ttaatctgtg atgcttattc agacactgat ggttttactt gggcaaatag
    32761 cctcgtcaat gaaaattgcc ttgtttttgg tttttcttta tctggcaaaa caaactctgt
    32821 catcacagca cttcatcaag ctagtcagcg aggggccaaa actgtcctct taacaacaga
    32881 taatcaaacc gaatttgacg actccttaga tattattccc gtatcatcaa cccaccaact
    32941 acattatggg aatcgtgtgt caccacaatt tcctcttctt attatgatgg acattattta
    33001 tgcttacgtt ttagcgattg acaaaccgca caaagaaaaa atcttcaaaa acactattat
    33061 tgacaaagaa aataacggcc tttagactac aatagcaaaa agggcgttgt tttcttttta
    33121 aagggtcgtc attcacctcc cccagtatac ttaaacaaga actcttcacg tcatatagac
    33181 tcacagccac taagattagc tactcgctaa attattttcg attttctcaa gagttataaa
    33241 tgaacaggat cgctcattct tttgtttaaa atggtagtaa gctcctaata taccagcatt
    33301 atttccatga ctagcaaact tcagctgagt tttcttggca agactagaaa ccaagtagct
    33361 atcaagagct gtctttaatt tatcagctaa atagtctttt tgcaccatga ttccgccacc
    33421 aagtaccacc acatttgggt taacaacgta gcagatatta gcaatacctt gtcctaagta
    33481 atccacctgc ttactaatgg ctgctataca aatcgcatca cccgctttag cctgctcaaa
    33541 aatacgacga ccatcccatt ggctaatgtc atccccataa gctaaggcca cctcttgaac
    33601 aagagctgtg gtagatgcca gatcctgaaa ctgaccatct gacaaatgaa ggtaaccaac
    33661 ttcgcaagct gaatgactac taccatgaaa aacttgggaa ttaaacaata aacagccacc
    33721 gattcccgtc ccaattgtca agcaaagagc cacaggataa tccttggcac tgccagaaat
    33781 agcttcggct agaccagcac agttaacatc gttttccact tcacacggta acccgaaagt
    33841 ctcttcaatc tcttttttaa actgagttcc agcatagtta ggaatctgag ggccggcata
    33901 aaagatttct cccttatcag gattaaccat tcctgcagaa gaaatggcca ctccagccag
    33961 atccatttgg tcttgatagg tctttaccaa gcccttgacc ttttccaaaa tagagggacc
    34021 acctttataa gcctcagtgg ccatttcctc tttttccaac aggtctcctg tttcagaaat
    34081 caagccatac ttgatagcag taccaccaat atcaattgct aaataatgct tcatttgtta
    34141 tcccctcgca tcttattaaa aggtttcctt agcctgatta atcagagcag cagcccgttg
    34201 gcaaataacc ctatcttcct cgaccaactc agctaaaggt gatcgaacag aaccaatatc
    34261 aagaccctca ttaatacgaa gtacttcctt aataacccca tacatattgc cgtgagcaga
    34321 aacaaggaca ccgatagtct cattaatagt atattgaagg gtcttggcct tttctaaatc
    34381 cttgtcaact ataagttgat tcagccttaa aaataattct ggcatagccc cgtaggtgcc
    34441 accaatgcct gcagcagcac ccatcaagcg ccctcctaga aattgttcat ctgggccatt
    34501 aaaaacaata tgatcgtctc cgccaatcgc acagaaggtt tgaatatcct gcactggcat
    34561 ggatgaattt ttaacgccaa tcacgcgctt attagccaac atagtcttat aaaggcttgg
    34621 ggtcaaggca acccctgcca gttgaggaat attatagatg ataaaatctg tgtgaggagc
    34681 agccgaagaa atcgcattcc aataatcagc aactgcatat tctggtaacc ggaaataaat
    34741 aggcggaata gctgcaatgg catccacgcc caaacgttct gaatgagctg ccaattcaat
    34801 actatcttta gtgttgttgc aggcaacgtg attaataatc gtcagcttgc ccttagcgac
    34861 tgccatgaca ttttctaaca ccaattgacg gtcaaaaacg ctctggtaaa tacactctcc
    34921 agaagagcca ttaatgtaaa gcccctgaac ccctttgtca atatagtact gggtcaaggc
    34981 acgcacacgc tcagggctaa tagctccttg gtcatcgtaa caagcataaa aggcaggaat
    35041 aatgccttgg tatttcgtaa gatctgtcat agtaatgtcc tttattcaat gtcattatta
    35101 agtgggaatc gcctaagaag ggattccatg ataatcaagt agaggtaagt taggctactg
    35161 atgcccatag tggccagtag agacaagcca caaaataagg ttaacagggt taacggcaag
    35221 gtaaaaacca aaaggccaat caccactaga aaagcaaacg tccatgggaa taaaattcct
    35281 gccagcagta aacttcgctt aaagagtatt aaaagaggaa gctcttttct gacggcctga
    35341 ggataagcgt acaaaaagag tataactacc aaaaataaaa cagcaatgca aagtacttta
    35401 aacccttgaa aaactaggcc tgattggttt cgaattaaga aaagatctaa gacacaaata
    35461 ctcattatca aaagctcaat aagacctagg cgcatcccac ttttaaaata atgtttaaaa
    35521 tgagagctat aagtagtaac aagctgacta acttgacctt ctcgccaatc caacaaactc
    35581 gcatacaaag ccattttagc aaccccaatc gttagaaggg gaaggcagga caagacaaat
    35641 aagagattaa acacgagcaa atcaaacagt gctgcgctag ccctcatcca tttactatca
    35701 agcttgaaga gagagtgtaa aagtccctgt tttttcgatg tcaaatcaca acttcctttc
    35761 tagaaatacg cttacaaaca aaagctaatt cccctcttaa acgtttaaca catgacttgc
    35821 ttcccatctg atttatctat gtatgcacct aaattaataa gcaacctata aattaagctt
    35881 agcgacacgc tttatttagt ctaacctttc acagcaccca tagtaatgcc ttgggtaaat
    35941 gatttttgga agacaagaaa taccgtcaca ataggcactg cagccatggc agcccctgcc
    36001 ataatcaagc cataattagt agccatttca gcttgcatag tggcaacccc aagtgaaatc
    36061 gttaaatttt cccgagaggt taacatcacc aactgcataa aataatcatt ccaagtattg
    36121 ataaaggtaa aaattgctag cgctgcaaag cctggtttga caatagggaa agccacattg
    36181 aaaaaggtac gaatttcgcc acatccatca atcttggcgg attccaataa ctcggttgga
    36241 atattttctg agaactgttt cataaggaaa acaccaaatg gccagcccac aagaggcaaa
    36301 ataacagccg ccaaagtgtc atggattccc ataaaattaa caatccgcac aagaggcact
    36361 agaacaacct gttttggcaa ggccatagca gcaataaaaa tcgaaaacaa aagccgctgt
    36421 ccataaaacc gctttttggc caaagcatag ccagccaaag aggaagtgcc acaaaccaag
    36481 aacatggtcg ccaccgaaat aaagacacta ttccacaacc attttaaggc tggattttgc
    36541 actaccaagg ccttaaaatt ttcaatagta ggtgcctttg gccaccattg cggtggaatc
    36601 ataatggtat cggcttgagc cttaaatgct cctgtcataa tccaataaaa tgggaaaata
    36661 aacaaaatgg tcaagatgca tagaataaca gtagtcagaa catccgatgc ggttagtttc
    36721 tttttcgtca tcttaatatc ctcctaatat tccacatcat tacctaaaat tttaaattga
    36781 gcaaagctga tgattgcaat catgactgcc aagaacacac ccattgtgtt ggcatagccg
    36841 tattcagaga gcttaaaggc tttttcatac aaatagtaca tgagggtact agttgaataa
    36901 tttggccctc cagacgtcaa gagttgaatc aaagcaaaac attggaagga attaatggtt
    36961 gttatgacag caatatataa ggttgttggc aggagacttg gccacttgat attccaaaac
    37021 acctgaaatt ctgtggcgcc gtctacacgc gcagcttcca tcaaagaatt atcaatattt
    37081 cccatagcag caatatatag aataataggc tgcccaacag atgtggttaa taaaatagca
    37141 ataatagcta acaaagccca gtgcttatct cccagccaac taatgttttg ttcaatcaca
    37201 tgagcatatt ttaagacata attcaaaatc cctgacatcg gatcataaat ccatttccaa
    37261 accaccgtta cggcaacact tccggtaaca acaggtagaa agaaaactgc ccgataaaac
    37321 gaacgcgcca ccacattttt gtcataagtc ttagccgcca caaagaggga aaagaaaact
    37381 acaacaggta ccgaaccaat aacgataatc aacgtattga taagagattt cataaagata
    37441 ggatcttgaa acatcctagc ataattggca aaaccaacaa aggtaaaatc cgtcatggaa
    37501 taattgaaaa aactcgtcac aaagcccata atcatcggta tcaagacaaa aatcacaaag
    37561 aaaaccaaga ccggagctaa gaaagcgtat gagatgagcg tctctcgcat ttttaattta
    37621 ttgacgttca cttgaaatac ctcttttttc tgttcaactt ttctaaaaag aaaagcttct
    37681 ttccagtgac catttgacat gtgcattgaa aagaagccac ttgttctgaa cacttattgt
    37741 gtttttttga ttgtcttgtt cgctttttca gtgaaggctt tcaaagcatc ttctggcttt
    37801 tcatcaccat tagagacggc ttgaaccatt gggaaccaaa gagttctcat ttcagcaaac
    37861 ccatcaatcg tgttgtagta tggtgagtag aattttgtcc attcagcaat tttttccatt
    37921 cgtttgtctt tgtaaagatc cccataagaa gttcttacag ggaaagcacc tgtacgaaca
    37981 acatttttag gtccccattc tttatcatca gcgataaact ggataaatgt cttagaggca
    38041 gcgacttttt gttcatcttt gttattaaag accgcaaaac catttacgag gtattctagt
    38101 tctggtttgc catcatctga tgggaatggg atttcaaggt aatccacttt actagcttct
    38161 aacaatttag cttggatgcc gggttgtgca ggcgcccaaa gaatggtaaa ggaagtttgg
    38221 ccattcgcaa agttttgaat atcagctgaa ccgtcgtatt gagagccatt catcattaaa
    38281 ccatctttaa tccaattgga aattttagtc atggctttaa tagagttagc atcatctgtg
    38341 gtgtatttgg ttactttgtc atcggtaata tgactactat aaaggttagc aaagaaggca
    38401 cgtggtcctt ggtcaccacc ttgaccatta gcaaagaaag aacctgggtt atatcctttg
    38461 tccttcaatg ctttcaagac tttttcaaaa tcatctgttg tccaaccctc tttcacaagg
    38521 tcaaggacac cagcatcttt gagcattttt ttattcaacg ccatgtagaa tggagcagag
    38581 ctgataggat acatgtaagc tgtatctcct gcttttgagg cttgaatcaa tttgtcattg
    38641 ttaacatctt tggtaaactc ttctgtgaac aagtcattca agtcagccaa tttcccattt
    38701 ttaccatatt gaatgatacg acctggagca tcaaagagga catcaggagc tgttccagct
    38761 tcaatagcag ttgtgatttt ctcaggacca gatgtaaaat caatggtttc caattttacc
    38821 ttgatttctg gattagcttt ctcaaaggca gcaatcaatt tcttctcata ggttcccaca
    38881 ccatcttcag ctttttcttg cgtgaagacc gggaaagccc accatgtgat ttctgtctta
    38941 gcagtatcag atttacttgc accagcctct ttctggctct ttccgccaca agctgctagt
    39001 cctaaaacag atgcaccaag catcactaat gaagctaact tcttcatgtt catcgtaaaa
    39061 tctccttttt ttgatgtgtg tttctatttt gacaaccact attaggattt gagcgcttcg
    39121 ataaaacgct ctgcaatttc cttaggcctt gtaatggccc ctccaacaac aatacctgcc
    39181 acacctaagt cattaatttt cttggcttct tctggggaat gaatctttcc ttcggcaatg
    39241 acagcaatgc ctgctttgca aagagcttct atcaatgcca catcaggacc tgcttcctga
    39301 cggctatacg gggtgtagcc tgacaaggtg gttccaacaa aatcaatgcc agcctgatgg
    39361 gccactagtc cttcatcaaa ggtactaata tctgccatca aaagctgatt ggggtacttt
    39421 tccttaactt gtcgaataaa gctagcaata tccaagccat catgtcggtc acgcttggta
    39481 caatccatag caatcactgc aatatttaag gcagctaatt gatccacttc ggccatcgtt
    39541 gccgtaataa aaggctcttg cggcgggtaa tcttttttga tgataccgat aattggtagg
    39601 tctgttatgg cttgaatttc tttaatgtct ctaaccgaat tagctctgat gccgacagca
    39661 ccagcttctt gagcagcttt tgccagcagt ggcataatac ccccagtctc tgaatagaga
    39721 ggctcacctg gcaaagcctg acaagaaacg ataatcccac cctttaactg ctccatgagc
    39781 ttttctttgg ttggtttatc aggcatagtt tctactcctt gtgtttctta gttttaataa
    39841 aaataacgtt cacaaagtta ttataacaaa aaataaagcg ctttcaatag caatgagact
    39901 tttagaatct actttcattt tttatattat tttctattat ttggaatgct ctttcataca
    39961 acaaataaaa aggtaaggag ttgacaccat acttatacat tgcaaattcc cgattagaaa
    40021 acgttaaaaa accaagagcc tcgagcactc ttggtttact ttagttgtat tttttgcaat
    40081 taagctgata atactttacg tcctttacga cgacgtgcag caagcacgcg acgtccgttt
    40141 ttagttgaca tacggtgacg gaatccgtgt ttacgttgac gacggatttt acttggttga
    40201 taagtacgtt tcacagtgaa tacctcctca tagattttgt attcgtttag ccggctattt
    40261 gtgatatcag ttactaaaca tactataata ttctatctta aagagggtct actgtcaaga
    40321 tttattttcg tttttcaaca gtttctctct catttaagag gccgctcatt tcataaagtg
    40381 tctcttcttt gacactgttc ctttgcctta ccgttgaaac cttatcaata gcaacaataa
    40441 tcaatgagat cagtaaaaca aacggttaag ctggccatac aaaaaatctt cgatagctaa
    40501 actaactacc aaagattctt tttaggatct aacgttgaag actgactacc acatagcggt
    40561 tagggtcatt gccttcagaa taactatcaa ccccttcaat ggatgaaacg gttttatgaa
    40621 cgattttacg ctcactgtta ctcatgggat ccatggtgta atcttgacca gattccaaga
    40681 cgcgtttggc aactttttgc gtaaagtcaa tcaaggtttc tgttcgatgt tccacataat
    40741 catggacatt caaggatact gaaaaatttt ttgaatagcg gtcgtgcaag aaattttgag
    40801 ctaacaactg caaggatttc aaaaccttac catggtaacc aatcacacgt cccgcctctg
    40861 gcgtttcaat ctgcaaattg atttggcgac gattgttgct ggtttcaacg gtggcttcga
    40921 tatccatttc atagatgatt ttagtcacat aatcggacac ttcctgagca gctttttcaa
    40981 tatctttgct gaaaccatca ttaccaggat cttggggcac ttctgattcc tcgactggga
    41041 ttgacggttg ttgtgctttt gtttcagtga ctgtctcagc aagattagaa agggtttgcg
    41101 ctggactatc ttcagacatg agaggaacca ctttggtatc ttctgcttct aacttttgag
    41161 tggcttttat ttcttcaggt tctacatcag cagaattgac cgcgggagca tttttttgat
    41221 taatatcctc tggaacacct cgagtggctt ttttatcagc cttataaacc gtcttgtcgc
    41281 tgataccttc gatatcaact tgggctggct ttttaccgaa cccgaggaaa ccttttttct
    41341 cttttgaaat gactttgata tgcgctttaa ggcgtgataa ccctaactct tgaagtcctg
    41401 tttcgatagc ttcttcgact gtttttcctg taaataatac cataacctaa actcctattt
    41461 tctcttcatt gcttttttac gagcacgacg ttcacgaaga cggcgttctt tttcttcatt
    41521 ggcaagacgc tgtctttcgg caataatctt aaaaggattg tttaatagca gtagttgtac
    41581 cacttgaaag gcgtttgaga ccgtccaata gagcactact ccactagcca agttaaagcc
    41641 catgaaaaag atcattagcg gcatcacata aatcataaca gtcatcatga catttttttc
    41701 tttagccgct agattggtca accaagtgga caagaaagtg aagacagctg ccaaaactgg
    41761 taaaaggtac aaatgatcat gttgtgccaa ttccacccac aagaaagtcc ctgtttttaa
    41821 gaaagatacc cgtgtcaagg cttggaatag ggcaatcata actggcatct gaattaagag
    41881 gggtaagagg ctagcataag ggttaacccc atattttttg taaagggctt gactttcttc
    41941 agccaacttc atccgcgttt gtgtgtcttt accagcgtat ttcttttgca attctctaag
    42001 ctcaggttgg atgtcttgca tcttctggct tgatttgatt tgcatattaa acaaaggcat
    42061 gagcatgaga cggatagtaa gggtaaaaag aataatgcca acaccaattg aaccatcaaa
    42121 ggaaagccat tgaattgctc tggcaaataa gtaaaccaac tggtcccaac cactagatga
    42181 ttgtgccgtt acctcaccac gaccacaggc cactaataat agaactaagg ggactattct
    42241 tgcaatttta atgtttttct tcactctcaa aacctttctc aagcaattgt gctaacttta
    42301 aaacatgatg taaattttgt tgaagctctt gatactccaa agactcgaca cccttacggg
    42361 caatcaccac gaaatcctct gacttcagct gatgccctaa tgccatgata acatgacgta
    42421 tctttcgttt gactgcattt ctggtgactg catttcctat ttttttaccg acagaaatac
    42481 ccacacggaa gtggtcttgg cctctattta aatgataaat gacaaatttt cgatttgctg
    42541 tactttttcc atccttaaat atggcttgga aatctttctc acgcttgaca cgataggtct
    42601 tcttcaaaat ttaactccaa tatctaaatt attaccatta taccacatcc cctaaaaaat
    42661 taaaaagccc taaccctttc agattaagcc tctttctgta ctatttctag tttttgtgct
    42721 tcaactgcct cttgtcactc catcttttcg tagtatagtt cttctttcga caaaggaata
    42781 accttcagaa aacaaccata aaaagtcaga atccacaaga cgtcagctac ttatccctgt
    42841 atttacctat caaaaaaccc ggcaagccgg gctttgatac tgattttaca atcagctaat
    42901 gtgatatcca aacaaagaca actgacatcg tcattttgtc tgccgagcca tcatatcaaa
    42961 aatactattt gtttatggag agagagaaaa caatattttg attgaattgg ggattgattg
    43021 ctcgataaac tcatgacttc gtaaatcttt atttacacta attattatag cgaaaaatgt
    43081 ttcaataaga gcatcatttc ctgaaatgtt gctttttcgt cctaaaatgt tattgctctc
    43141 ttaactttag cgaagagcct cccaccgttc aaacagacat ttcattttta atcgtgatac
    43201 catacaagaa tcaccatttt cgaaataaag gagctgctta ctcttatcaa ttcgaacaac
    43261 cttatctaga ttcacaaggt aggatctatg gcacaaaaaa agtttcggtg ctactgcttg
    43321 aatctcagat agattaccat aaaactctaa tctttctgtc tgagtccata aacaaacttt
    43381 gtgaggagtt gtggctgtag caaagtataa aatatcctta tagggcaatt ttaatcttgt
    43441 ctgtggagtc tcaaataaaa acatgtcctt tgactcacga ctagacatca tatcgtaagt
    43501 atatcggata cattcttcga tttgttcttt aaactgtttt tgaccacctg ctttatcaat
    43561 aaaatctaga gcggatacct tatatttaaa agaaatagga gcaaattctg aatgagtggt
    43621 gacaaacaca ataacggcat taggatcttt ttgtcgaata gctgcagcta attcaagccc
    43681 acaacgagta tattcaccaa tttcaatatc taaaaaatac agctgatggt ctcctctttc
    43741 ttggatactc tcaaataatt tttgaggagt cgagaacact tcaagctgat tacatggtat
    43801 tctagtctct tttaagatgc ctactacgat agattctatg cgtgtctgct ggataaaatc
    43861 atcctcaaga ataaaaatgt tcatcatact ttatctcatt tctaacaatt gtcttaatcg
    43921 ataatggtca cttttagtcg aaagcgttat ttgggggtaa ctatctaaaa cagtcaaaaa
    43981 gtgggctgag ttctgctgct gagcatcaaa gcgtttggct atatccacac gattggtttt
    44041 tgtgctattt tcaataataa ataattgctt gttatcttgt ttaaagtatg caatagataa
    44101 aaaaggacgt ctactgcctt tggctgtctc aatagcattg tccactagtc cagtaaaaag
    44161 tacaattaag tcgattaact taataggtaa atgatcgata acatctggaa tctcaatata
    44221 aagttcaata cccaaatact gagcttcaag caatttggaa ttcatgatac tgcgaatcac
    44281 agcctctttg atattgtcta aagaagatat ttcataattt gtgcgcatca tagaagttgc
    44341 cgattgctga ataaccgtgt cataaactct agtaatcaga tctatatcac cactatcgat
    44401 actatctttt agactaatta agatattttc agaatcatgt ttaactgttc tgatttctcg
    44461 gtaaagttgc tcaatcgatt gattgtaatt ttccagatac gcaatacgtt cattttgtgc
    44521 ttgtgttaat ttttgtgaca gctgatcttt agcaaatcga tctaatttta caata
//
LOCUS       DATUYR010000010        41272 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.010, whole genome shotgun sequence.
ACCESSION   DATUYR010000010 DATUYR010000000
VERSION     DATUYR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41272
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.010"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            81..335
                     /locus_tag="VCI33_000464"
     CDS             81..335
                     /locus_tag="VCI33_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEP2831407.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            356..751
                     /locus_tag="VCI33_000465"
     CDS             356..751
                     /locus_tag="VCI33_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEP2831408.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            1037..1356
                     /locus_tag="VCI33_000466"
                     /pseudo
     CDS             1037..1356
                     /locus_tag="VCI33_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1353..1916
                     /locus_tag="VCI33_000467"
     CDS             1353..1916
                     /locus_tag="VCI33_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP2831409.1"
                     /translation="MNLIIIGAQASGKMTIEQEVARQTGMNLFHNHDSIDFVLRFMPW
                     SQESTALIERMRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTHLTAAETAQLIIQKMTHIKEN"
     gene            1918..4566
                     /locus_tag="VCI33_000468"
     CDS             1918..4566
                     /locus_tag="VCI33_000468"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEP2831410.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDNRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIV
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVERLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVIQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            4720..7128
                     /locus_tag="VCI33_000469"
     CDS             4720..7128
                     /locus_tag="VCI33_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEP2831411.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWDYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDITDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            7261..7989
                     /gene="cas5c"
                     /locus_tag="VCI33_000470"
     CDS             7261..7989
                     /gene="cas5c"
                     /locus_tag="VCI33_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEP2831412.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            7989..9884
                     /gene="cas8c"
                     /locus_tag="VCI33_000471"
     CDS             7989..9884
                     /gene="cas8c"
                     /locus_tag="VCI33_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEP2831413.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANREKLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            9889..10737
                     /gene="cas7c"
                     /locus_tag="VCI33_000472"
     CDS             9889..10737
                     /gene="cas7c"
                     /locus_tag="VCI33_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEP2831414.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLERRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYHQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            10739..11413
                     /gene="cas4"
                     /locus_tag="VCI33_000473"
     CDS             10739..11413
                     /gene="cas4"
                     /locus_tag="VCI33_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEP2831415.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMQEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            11410..12435
                     /gene="cas1c"
                     /locus_tag="VCI33_000474"
     CDS             11410..12435
                     /gene="cas1c"
                     /locus_tag="VCI33_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEP2831416.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGCASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILMTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            12446..12739
                     /gene="cas2"
                     /locus_tag="VCI33_000475"
     CDS             12446..12739
                     /gene="cas2"
                     /locus_tag="VCI33_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEP2831417.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   12887..13049
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12888..12918
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaa"
     gene            13210..13920
                     /gene="ccdA2"
                     /locus_tag="VCI33_000476"
     CDS             13210..13920
                     /gene="ccdA2"
                     /locus_tag="VCI33_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEP2831418.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHIFLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            13933..14556
                     /locus_tag="VCI33_000477"
     CDS             13933..14556
                     /locus_tag="VCI33_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEP2831419.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMVKKEITQDKMSMAVKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFTKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            14599..15705
                     /gene="msrB"
                     /locus_tag="VCI33_000478"
     CDS             14599..15705
                     /gene="msrB"
                     /locus_tag="VCI33_000478"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP2831420.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKVIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVTYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVVRYKEDKSFNMTRTEVRSRSGNSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            15793..16533
                     /locus_tag="VCI33_000479"
     CDS             15793..16533
                     /locus_tag="VCI33_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEP2831421.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLQQKLDLSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKDLGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            16530..18263
                     /locus_tag="VCI33_000480"
     CDS             16530..18263
                     /locus_tag="VCI33_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2831422.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNSQLTMKTSSDMMAEPWYKAAIDRQAMPVLTPARQLSLSS
                     KKEWVVSVTQEVVDRAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTNLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLVLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLTLPKMH
                     ELMADDTQENE"
     gene            18336..19415
                     /locus_tag="VCI33_000481"
     CDS             18336..19415
                     /locus_tag="VCI33_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEP2831423.1"
                     /translation="MSLASDYFSRQTPIVEKLMAYGFEKRNNGYFYNERFMEEEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVEGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKAFPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETNIPNEGYRKNPNIDKLMRLRKRQQYKDGLLSFDLMKKHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            19429..20145
                     /locus_tag="VCI33_000482"
     CDS             19429..20145
                     /locus_tag="VCI33_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEP2831424.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(20311..20784)
                     /locus_tag="VCI33_000483"
     CDS             complement(20311..20784)
                     /locus_tag="VCI33_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP2831425.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATICGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            20926..21606
                     /locus_tag="VCI33_000484"
     CDS             20926..21606
                     /locus_tag="VCI33_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP2831426.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQYTK"
     gene            21880..23115
                     /gene="arcA"
                     /locus_tag="VCI33_000485"
     CDS             21880..23115
                     /gene="arcA"
                     /locus_tag="VCI33_000485"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEP2831427.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            23215..23643
                     /locus_tag="VCI33_000486"
     CDS             23215..23643
                     /locus_tag="VCI33_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2831428.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQSYGPKCLEDG
                     EYCQSLAKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNN"
     gene            23683..24696
                     /gene="argF"
                     /locus_tag="VCI33_000487"
     CDS             23683..24696
                     /gene="argF"
                     /locus_tag="VCI33_000487"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEP2831429.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMNLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            24866..26359
                     /locus_tag="VCI33_000488"
     CDS             24866..26359
                     /locus_tag="VCI33_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEP2831430.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            26376..27707
                     /locus_tag="VCI33_000489"
     CDS             26376..27707
                     /locus_tag="VCI33_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEP2831431.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPEGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTDPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYKG
                     NKLEALQKELDKLGFEYVVKEGELTVYGLAQHTKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            27727..28677
                     /gene="arcC"
                     /locus_tag="VCI33_000490"
     CDS             27727..28677
                     /gene="arcC"
                     /locus_tag="VCI33_000490"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEP2831432.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALISTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKSLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYINFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            28990..29982
                     /locus_tag="VCI33_000491"
     CDS             28990..29982
                     /locus_tag="VCI33_000491"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEP2831433.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            30105..30644
                     /gene="rsmD"
                     /locus_tag="VCI33_000492"
     CDS             30105..30644
                     /gene="rsmD"
                     /locus_tag="VCI33_000492"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEP2831434.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            30634..31125
                     /gene="coaD"
                     /locus_tag="VCI33_000493"
     CDS             30634..31125
                     /gene="coaD"
                     /locus_tag="VCI33_000493"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEP2831435.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            31112..32149
                     /locus_tag="VCI33_000494"
     CDS             31112..32149
                     /locus_tag="VCI33_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEP2831436.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDTDFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            32564..33541
                     /locus_tag="VCI33_000495"
     CDS             32564..33541
                     /locus_tag="VCI33_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2831437.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDCVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFESIQ
                     LEASYNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            33965..34609
                     /locus_tag="VCI33_000496"
     CDS             33965..34609
                     /locus_tag="VCI33_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEP2831438.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            34613..35692
                     /gene="rlmN"
                     /locus_tag="VCI33_000497"
     CDS             34613..35692
                     /gene="rlmN"
                     /locus_tag="VCI33_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEP2831439.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(35774..36400)
                     /locus_tag="VCI33_000498"
     CDS             complement(35774..36400)
                     /locus_tag="VCI33_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEP2831440.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQLQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            36628..37155
                     /locus_tag="VCI33_000499"
     CDS             36628..37155
                     /locus_tag="VCI33_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEP2831441.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            37297..37500
                     /locus_tag="VCI33_000500"
     CDS             37297..37500
                     /locus_tag="VCI33_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEP2831442.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            37505..38476
                     /locus_tag="VCI33_000501"
     CDS             37505..38476
                     /locus_tag="VCI33_000501"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEP2831443.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            38486..38875
                     /locus_tag="VCI33_000502"
     CDS             38486..38875
                     /locus_tag="VCI33_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEP2831444.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRAKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            39058..40899
                     /gene="typA"
                     /locus_tag="VCI33_000503"
     CDS             39058..40899
                     /gene="typA"
                     /locus_tag="VCI33_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEP2831445.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            41021..41272
                     /locus_tag="VCI33_000504"
     CDS             41021..41272
                     /locus_tag="VCI33_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEP2831446.1"
                     /translation="MFYLIMAVSIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
BASE COUNT        13050 a         7308 c         8788 g        12126 t
ORIGIN      
        1 accagttatt aataacatgg cacaagaggc ctgccttatg gtataatggt aaagatgatt
       61 tcagaaagag gaagctaagc atgtcttatg aaaaagaatt tttaaaagat tttgaagact
      121 gggttaaaac tcagattcaa gtcaatcagc tagccatggc aaccagtcaa gaagttgccc
      181 aagaagatgg cgatgaaagg gctaaagatg cctttattcg ttatgaaagt aagttagacg
      241 cctacgaatt tttactaggt aaatttgaca attataaaaa tggtaaagcc tttcacgata
      301 ttccagatga gttatttgga gcaaggcatt attaaggaca aggagacacg aaatagtggc
      361 aaaacaaaaa aatcgtaaaa aagcattgaa gttagaaatg aagcgtaatg gcttgttaaa
      421 aaaagcaggt caagtttttg ataaggcagt tgaaagtgtt gaaacagccg tggataaaac
      481 catttctgct ggtaaaaatc ttgtggaaaa aggcagccaa acggttgaaa acctgacagc
      541 aagcaaggaa agacttactc ttgatgagtt tgcagggcag ccagttgtag ctggtatccg
      601 ctctgacctt gtcgaaaccc tatatgcaga aggcatccat tcagcgcaag cttttaagga
      661 gtggacagaa aaagacctcc ttgctcttaa gggcattggc ccagcaacgg ttaaaaaatt
      721 ggtcgaaaat ggcgcaagct ttaaaaaata gtcatcttct cgttgcttaa tggctataaa
      781 tttgttagaa tattagcaat cagaggtgca ttgagttggt ctgttacaga gagcggtggt
      841 gttgagaaat ccgcacagat gtcaaggctg gtagctgagg ataagaaatc agatatgaaa
      901 agtagcagat ccattttatg gtctgaaaca gtggtggtac cgcggaaatc gtaacacgaa
      961 attcgtccct gtccagtgta agctggatag ggattatttg gtttcatttg aaagaagatg
     1021 atggcttatt gttaaaatgt tgttagaagt atacaataga aaaactttgc ccaaagtgga
     1081 aacagaacgg ctgctattac gtcaacgaaa aggggacaag gcattattcc agaagcggct
     1141 tcagctctga ttgaaattgg ttttacctta cttgatttgc ataaaattga gctgggttgc
     1201 tacgactaca acaagcaaag tcaggcagtg gctcgcaaat taggctttac cttagaagcc
     1261 aatgtccgtg agcgtaagga cgttcaaggt cgccgctgtg gcggcatgcg ttttgggtta
     1321 ctgagaagtg agtgggagga gcagaaacaa aaatgaacct tatcatcatt ggtgctcaag
     1381 cgtcgggcaa gatgaccatt gagcaagaag ttgccaggca gacaggcatg aatttgtttc
     1441 ataatcacga ttctattgat tttgttttac ggtttatgcc ttggtctcaa gagagcactg
     1501 ccttgattga acgtatgcgt tttgcttttt ttgaaacctt tgctaagaca ggtcaagaca
     1561 tgatttttac tattgtaatt gacttcaatg atccaaatga tgtagcaatg cttgaaaaga
     1621 ttcaggctgt ttttcagtct tatgaccaag aggttctctt tgtcgaatta aaaacagata
     1681 tagaggaacg tcttaaacgc aatcgcacag aaaatcgtct caaacataaa cctcttaaac
     1741 gcaacatcga gtggtcagaa caagatattc aatccacgat ggcatatgct gtttttaatc
     1801 cagaagaacc acccaaaacg ctcacgcatt accaaaaaat caataatacc catctcaccg
     1861 ctgctgaaac agcgcagctt attatccaaa aaatgactca tatcaaggag aactaagatg
     1921 acagaactat cacctaaata caatcctgcc gaggttgagg caggtcgcta tcaaaaatgg
     1981 cttgatgcag atgtttttaa gccatcagga gaccaaaaag caaaaccgta ttctatcgtg
     2041 attccaccgc caaatgtgac tggtaagttg cacttaggac acgcctggga tacgacgctt
     2101 caagatatta tcattcgtca aaaacgcatg cagggctttg atacgctttg gcttccaggg
     2161 atggaccatg caggaattgc gacacaggct aaggtagagg agcgtctgcg cgagcagggg
     2221 atttctcgtt acgaccttgg tcgtgacaaa ttcctagaca aggtctggga gtggaaagac
     2281 gaatatgcca ctacgattaa agagcagtgg ggcaaaatgg ggctttctgt ggactacagc
     2341 cgtgagcgct tcacccttga tgagggcttg tcaaaagcag ttcgcaaggt ttttgttgac
     2401 ctctacaaaa aaggctggat ttaccgtggt gaattcatca tcaactggga tcctgctgcc
     2461 agaactgccc tttctgacat cgaggttatc cacaaggatg ttgagggagc tttctatcac
     2521 atgaactata tgttggaaga tggctcacgc gcccttcaag tggcaacaac gcgaccagaa
     2581 accatgtttg gagacgttgc cgttgccgta aacccagaag atcctcgtta caaagatttg
     2641 attggcaaaa atgttatttt accaatcgtg aataaattga ttccaatcgt gggtgacgag
     2701 catgctgacc ctgaatttgg gactggagtt gtgaaaatca cgcctgccca tgatcctaac
     2761 gatttcgagg ttggtcaacg ccataatcta ccacaagtga atgtcatgaa cgatgacggt
     2821 accatgaatg agttagctgg cgactttgca ggcatggacc gttttgaagc tcgtcaagct
     2881 accgttgcta aacttgaaga acttggtgcc ctagtaaaca tcgaaaaacg tgttcactca
     2941 gtaggtcact cagaacgttc tggtgcagtc gttgagcctc gtctatccac ccaatggttt
     3001 gtaaaaatgg atgagcttgc taaacaagct atggacaacc aagaaacaga taaccgagta
     3061 gatttctacc caccacgctt taatgatacc ttccttcaat ggatggaaaa tgtccatgac
     3121 tgggtaatct cgcgtcagct ttggtggggg caccaaattc ctgcttggta caatgcagaa
     3181 ggtgagattt acgttggcga agaagcacca gaaggtgatg gctggactca agatgaagat
     3241 gttcttgaca cttggttctc atctgcttta tggccgttct caactatggg gtggcctgac
     3301 acagatgtag aagatttcaa acgttatttc ccaacctcaa ccttagtaac aggttatgac
     3361 atcattttct tctgggtgtc acgtatgatt ttccaatcgc ttgaatttac cgggcgtcag
     3421 ccattccaaa atgtcctgat tcacggtctt attcgtgacg aagaaggacg caaaatgtct
     3481 aaatcattgg ggaacgggat tgatccgatg gatgtcattg agaaatatgg tgccgatagc
     3541 ttacgttggt tcctctcaaa cggctctgcc ccaggacaag acgtgcgttt ctcatacgaa
     3601 aaaatggacg cctcttggaa tttcattaat aaaatttgga atatttctcg ctatatcctc
     3661 atgaataatg aagggttgac cttggaagaa gctgaaagta atgtggctaa ggtagctgcc
     3721 tcagaagctg gaaatgtgac tgaccaatgg attctccaca atctcaatga aaccatcgct
     3781 aaagtgacag aaaactttga caagtttgag tttggtgtgg caggacacat tctttacaac
     3841 ttcatctggg aagaatttgc caactggtat gtggaattga ccaaggaagt gctatatagc
     3901 gacaacgaag ctgaaaaagt tatcacgcgc tctgtccttc tttatacttt ggacaagatt
     3961 gtacgtctcc ttcacccaat catgccattt gtaacagaag aaatttacgc tcaatatgcc
     4021 caaggttcta ttgtgacggt ggactatcct gtggttcgcc cagcttttga aaatgaagct
     4081 gcgcataaag gtgtggaaag gttgaaagac ctgattcgtg cagttcgtaa tgcgcgcgct
     4141 gaagttaacg tggcaccatc aaaaccaatc accattttgg tgaagacagc agatagtgag
     4201 ttggaagact tcttcaccag caacgtgaac tacatcaaac gtttcaccaa tcctgaaaaa
     4261 ttagaaatca gctcagccat tgctgcgcca gagctggcta tgaccagtat catcacaggt
     4321 gctgaaatct acttgccact agcagatttg cttaacgtgg aagaagaact cgctcgccta
     4381 gataaagaac tggctaaatg gcaaaaagaa ttggacatgg tcggcaaaaa actcggaaac
     4441 gaacgctttg tagccaacgc caaaccagaa gtcatccaaa aagaaaaaga caaacaagcc
     4501 gactaccaag ccaaatacga cgcaacccaa gaacggattg cagagatgaa gaaaattaaa
     4561 tcatagtgtt tagaacgcag ttgaaaaact gtgttctttt cttactaata gagatattag
     4621 attgaagttt aattatgaga aaaacgcttt ctttatttaa agagttatgt tacaatacta
     4681 ttacaatcat cgtacaaata gtgttatttt gaggggcata tgagaatgat tttagcacac
     4741 tatgactgta aaaaagataa aaagcaatct ttagatgagc atttatggca tgtggcctgt
     4801 tctagtcgac aggaagcatc tataattggt caaggagatg tgcttttttt aattggtctt
     4861 taccacgacc tgggcaaagc tgatcgaacc tttcaagata aattattaaa taatccaaat
     4921 cggcatgttg atcactctta tgcaggggca aaatacttat gttctattat tgggcctcat
     4981 ctaaaaaacc gaggggttga taaaaatgag agaatgacat tcaacgaaat ggtggggtat
     5041 gtcatctctg ctcatcatgg gatgtatgat ttatgctact attttgacga tgctgaatat
     5101 tatggcttta ataagtttaa aaatcgtatc aatagagact tagatggtta tcactatcat
     5161 gaagatatta aagggtacgc tctaaaatta gaaaaaaaat tatgtgatta tggctacaaa
     5221 gatttaaggg agcttattga taaagctttt gataattacc aacaagccat gtcttcctta
     5281 aactggcaag ataagagtga gtgggattat tatcagtctt gtatggtgag actttacttg
     5341 tcactcttaa aaaacgctga tattttggac acagtaaatg cctatggcct taagataagt
     5401 cctatggata aaacagagcg atcctttcta aaacactcct atttagcggc cattgaacaa
     5461 aaatatgcta gctttggaca gccaaacaat cagttgaaca ctattcggac agaaatcgct
     5521 gagcgtgtta aagaaagagg taaacgagat tccaagggga tttatcgctt agatttaccg
     5581 acaggagctg gcaagactaa tcttagtatg cgttatgcgt ttcaccaatt agttcatcac
     5641 gacaaatcaa ggttttttta cataacgccc tttctttcgg ttcttgaaca aaatgcttcc
     5701 gaaattagaa aagttacagg tgaccttggc gtcctagaac accattccaa tgtggtgaaa
     5761 caggctaatg aagatgatga tgacaaggac agtttattgt cagcttatct tagtgatagc
     5821 tgggacagtc aagtagtctt gacttctatg gttcaatttt tccaaacact tttcaaaaca
     5881 aaatcagcta atctgagacg tttttcaagt ttgattaata gtgttgtgat tctagatgaa
     5941 gttcaatccc tgcctattga agtcaccact ttgtttaatt taacgatgaa ttttttaaat
     6001 aaagttatgg atacaaccat cgttctttgc acagcaacac aacctgctta tgattcttca
     6061 gagattgacc atcgtatctg ttatggaggg aacttgggag aattagctga aatcgttgag
     6121 ttaacgattg aagaaaaaca gattttttca aggacagagc ttagaaaatt tgatgatagt
     6181 gatcagaaag ttcacttgac tgatgttatt aatcttattc taggtgagga aaactcagtt
     6241 cttgctattt ttaatacgaa aaaaacggtt cataactgct atactatgct aaaagacatt
     6301 actgatagac cggtctatca gctttcgaca aatatgtgtg cgcagcatag acttgacttg
     6361 attgctaaga tcaaaacgga gttacaaaat aatatcccta ttatttgtat tagcacgcaa
     6421 ttaattgaag caggtgtaga tgttgatttt catcgcgtca ttcgttccta ctcagggatt
     6481 gattctattg ttcaggctgc tggacggtgt aaccgagaag gcaaacgaga taaagggcaa
     6541 gtcactcttg tcaatctgac caatgaagag gaaaatattt ctaggctgac agaaataaaa
     6601 gctaaaaaag aagccacaga atctattctt cataagattg ggtctccaat tgatatctca
     6661 actttaaacc gtgacttttt tgagtattat tatgccaata atcagggact gatggattat
     6721 cctttggaag acaacctatc aatctacgac tatttaagcc ttaatattta tcagacggca
     6781 aataaaaagt tcaaaggtaa gttaaaacaa gcttttaaaa cagcaggagc caaaatgaac
     6841 ctcatcaata atgatatgat aggaattctc gtaccttatg gcgaagctga gaaaaaattg
     6901 gcttatttag aagaattggg tatgtctcac tttttatcag cagaagatta tcaaacgata
     6961 aaatcattac taaaagagtt acaacctttt acggttaatg tccgcgagaa cgatcctctc
     7021 tttgagacaa caaaatctta tctaaatggt cagattctgg ttttgacgtc ggagtattat
     7081 gacacggaaa gaggagttaa atacgattca gctagctttt acttctaact caaaacgaaa
     7141 gcagattaac aaaaggttgt tagaggacct tgttaacctg ccaatcatca ttaataatta
     7201 ttatcaattt agactattta ataaaattag attacaaaaa aacagaagga ggaaagtagc
     7261 ttgtacagat ctagagactt ctacgtgaga gtaagtggtc agcgagctct ttttacaaat
     7321 ccagccacaa aagggggatc ggaacgctca tcctattcgg ttccgactag acaggcactg
     7381 aatggtatcg ttgatgccat ctattataag ccgaccttta ctaatatcgt cacagaggtt
     7441 aaggttatta accagattca aaccgaatta cagggtgtca gggctctgtt acatgattat
     7501 agtgcagatt taagttatgt atcctatttg agtgatgttg tttatctgat caagtttcat
     7561 tttgtttgga atgaagatag aaaagatttg aactcagata gacttccagc taaacatgaa
     7621 gccattatgg agcgttctat tcgtaaaggg ggacgtcgag atgtgttttt gggtacaaga
     7681 gaatgtttag ggcttgtaga tgatatcagc caagaagagt atgagactac tgtgtcgtat
     7741 tataatggtg tcaatatcga cttgggaatc atgttccatt cctttgccta tccgaaggac
     7801 aaaaagacac cattaaaatc atactttaca aagactgtga tgaaaaatgg agtcattacg
     7861 tttaaagcac agtctgaatg cgatattgtt aacacgcttt ctagttatgc ttttaaagca
     7921 ccagaggaga taaaatcggt taacgatgaa tgcatggagt atgatgccat ggagaaagga
     7981 gaaaactgat ggattttttt acttctctct tgaagactta tgaaaaagca gagctagcag
     8041 acttggttga tcaccaaaaa agaagtaatg agccggtttt actgccgatt tatcatacga
     8101 gtttaaagtc aaatggcaaa aatatcattt cagtgaaact tgacaaagat ggccagtttc
     8161 acaaggcaga atttatggca gataagcaaa tgattatttt tcctgtaacg gctgattctg
     8221 ttgctaggtc aggtagtcat cctgcaccgc atcccctagt cgataaattt gcttattata
     8281 gtgctgaaat ggggcagtct cagtatgatt ctttccataa gcaacttaat aactggattg
     8341 attattgtga ggagggtgac gtcaagaggt ttttaaccat tgttcagcaa tttattttga
     8401 aaccagaatt tctgacactg attctcgatt ctttaattgg tcctgattat caaaataatc
     8461 aattaaaagt cacattttgt gatgccactg gaaaagaaaa attaattgat ttatcagctt
     8521 gctttttaga attttcaatt gatcagttcc agggctttaa aaatgaatcg gtttcgacat
     8581 ttaaaacttt acaccaatcc tatatttctt ttgttgaagc caatcgtgaa aagcttggta
     8641 tttgtaatat tagtggacga gaggaacagc ttaccgataa gcatagaggt ttgatgggga
     8701 atgctaaaat catctctgtt agtaataaaa gagaagctta taaaggacgt tttagagaac
     8761 gtgaagatgt ttttagtgtt ggctatgaaa cttccgaaaa gattcattta atgctcaagt
     8821 accttttaga aaataaaaat accagtactt ggttagggtc ttctcaatat ttaatcaact
     8881 ggtttagtga tgatttaaca aatgatagtc ggttggatat cgtatcacca atctttgatg
     8941 acggacttga agaagatgat gatgacgata cgcctcctgt tataacatta gcaactgaag
     9001 acaataaaag aattggtaaa tcattcatca agggacaaaa attatttgct aatgatgcca
     9061 cttactacgt tgctattttg aataaaacca gcaatgggcg gattgcttta aaatattttc
     9121 gtcagcttca agcgtcccaa ttactcacca atcttaacaa gtggcaggaa acatacagtt
     9181 gggagtcgcg atctaagttt ggaaaaagtc gcttaagaac ccctactttt catgacatcc
     9241 ttaatgtgtc ctacggggtt gatagggatc gcttccttga attagataat gataacttca
     9301 aaagtgatca aattcaaaag ttagtggcaa gtttgattga tggtaaaccg atgccacagt
     9361 ccattgtcaa aaagttaggt aacaatgtta aagaacgaca tcgttaccgt aagcactggt
     9421 atcaagttga gcaggtctgc ttagcaattt tacacaaaca aaatggggag gaattttcac
     9481 cgatgctaga tcataccaat caaaatcgtt cctatctttt tggacgatta ttagcaattt
     9541 ttgaattaat cgagaccttg cgttatggct tggatggaaa caataacgac cgtattacca
     9601 atgctgaacg ttattggaca gcctatactg gacaaccaac aaaattgatg atgttattgg
     9661 aaaataaaat taagccttac gaagaaccat tgaaattaaa tcgtcgtggc agttggatga
     9721 aattagaaaa agaaaaagaa gagattttag aactgttaaa tcctctgtta gaaacagaaa
     9781 caatggaaaa acccttagat taccgcttta tttttgggta ttatgctgag aaaaactatt
     9841 actatacaaa acaaaacact gaagtaacag aaagtgagga gtaaaaagat gttggaacac
     9901 aaaattgatt ttatggtaac tcttgaagtg aaagaagcaa atgcaaatgg tgatccctta
     9961 aatggaaaca tgcctcgtac agatgccaaa ggatatggtg tgatgagtga tgtctccatc
    10021 aaacgtaaga ttcgtaatcg tttgcaagat atggggaagt ctatttttgt gcaagctaat
    10081 gaacgtattg aagatgactt tcgttcacta gaaagacgct tttcacaaca ttttacagct
    10141 aagacacctg acaaagaaat tgaagaaaaa gcaaatgcat tatggtttga tgttcgtgct
    10201 tttggacaag tttttactta tctgaaaaaa tcaattgggg tgcgtggacc agtttccatc
    10261 agtatggcta agtccttgga gccaattgtc atttccagcc ttcaaattac gcgtagtacc
    10321 aatggtatgg aagctaagaa taatagtggc cgctcttctg atacgatggg gacaaaacat
    10381 tttgtagatt atggtgtgta tgtacttaaa ggttctatca atgcttattt tgctgaaaag
    10441 actggttttt ctcaggaaga tgctgaggct attaaagaag ttttggttag cttgtttgaa
    10501 aatgatgcgt cgtctgcacg tccggaaggc tctatgcgag tttgtgaagt cttttggttt
    10561 acgcattcaa gcaaactggg gaatgtttca agtgcgcgtg tctttgactt gttagagtat
    10621 catcaatcaa tagaagaaaa aagcacttat gacgcttatc agattcatct aaatcaagaa
    10681 aaattggcta aatatgaagc gaaagggtta acggttgaaa tcctagaagg actctagtat
    10741 ggtctatgcc gaagatgatt atttaatgct atcaggtatt cagcatttcc aattttgtaa
    10801 acgtcaatgg gcgttgatcc atattgagca acaatggctt gataatgaag cgacagcgca
    10861 tggacaggtt ttacatacta aagcagataa cccttacatt aaagaaaaac gaaaagagct
    10921 tttggtctca cgtgctatgc ccatttcttc tgcagaactt ggactttcag gaattatgga
    10981 tgttgtggaa ttttataaag atgatcaagg tgtgtctttg aggggaaaac gtgggaaatg
    11041 gttaccaaaa gttgtggaat acaagcgcgg aaaacctaaa aaagatacca gagatattgt
    11101 ccagttggtg gctcagacca tgtgtttaga agaaacgcta gactgcgaca ttaacgaagg
    11161 ttgtctttat taccatagtg tcaatcaaag agtgattgtt cctatgacat cagctttgcg
    11221 tcaagaagtg aaggaattag ccgcagagat gcaagaggtt tatcagagtc aaatgctacc
    11281 taaagcagct tattttaaaa actgtcagct ttgttcttta gtcgatattt gtaagcccag
    11341 gttgagtaaa aaaacaagga gtgtgtcgcg ttacatcaat gaggctatga ccagtgagga
    11401 gatggaccta tgaaaaagtt gctaaatacc ttgtatttga cgcaagaaga tttttatgtc
    11461 actaaagagg gcgataacat tgttatcaag caagaaggta aggttctcaa acggtttccg
    11521 tttcggatta ttgacggtat tgtctgtttt tcttatttgg gtgtgtcgcc tgctttggtg
    11581 aagttatgta cggagaatca gattaattta tcgtttcata caccacaagg gcgtttttgt
    11641 ggtcgctata ttggttcaac caatgggaat gtgttgttgc gtagagaaca ttatcgttta
    11701 tctgatcgtg aggaatcttt ggaatacgca aagcggttta ttttggctaa aatttccaac
    11761 tcaaggaaat acttgctacg ctttaaacga gatcatcgtc aacagattga taccaagctt
    11821 tttgaggctg ttaatgacga attgatatgg gctttagaga tggttcaggc agcagataat
    11881 aaagactctt taagagggat tgaaggccaa gctgctaatc agtattttcg catatttaat
    11941 gacctggtgt tgacggacaa aaaaacgttt tacttccaag gtcggagtaa acgaccaccc
    12001 ttagattgtg ttaatgccct cttgtctttt ggttacagtt tactgacctt tgaatgtcaa
    12061 tctgccttgg aagctgtcgg attagacagt tacgttggtt tctttcacac ggatcgtcct
    12121 gggtgtgcta gtttagcgct tgatttagtt gaagagttcc gctcatatat tgtagatcgt
    12181 tttgtctttt cattaattaa taaaggacaa cttcagaaaa aacactttga ggttaaagaa
    12241 aatggtagta ttttaatgac ggaaaatggc agagctattt ttattgattt gtggcagaag
    12301 cgtaagcata ctgaggtaga acatcctttt acaaaagaga aagtaaaact tatgttatta
    12361 ccctatgtac aagcgcagct tttagctaag gccatacgag gagatttaga aagctatcca
    12421 ccttttatgg tttaggagat gttatatgat ggttttagtc acttatgatg taaatacgga
    12481 aacacctgct ggtagaaaaa gattgcgtca tgttgccaaa ctctgtgtgg actatgggca
    12541 acgtgttcaa aattctgttt ttgaatgttc tgtgacaccc gcagaatttg tggatataaa
    12601 gcaccgctta acacaaatca ttgatgagaa aactgatagt attcgctttt atttattggg
    12661 gaaaaattgg cagaggcgtg tggaaacact tggtcgctca gacagctatg acccagataa
    12721 aggtgtctta ttattgtaaa aatctcttgt gcgaagctag ctttcacaga aacaccttgc
    12781 ttgctcgcgc aaaaataact taaaaaagaa gcgaaatgga gataaaaagg cttaaaacac
    12841 ctctatgtgt gccatccgtt tctctataaa ctgtgtcatt tggcgcagtc tcacccttca
    12901 tgggtgagtg gattgaaata aataattaca tgactagcca atacacccac atagtctcac
    12961 ccttcatggg tgagtggatt gaaatttcat gagcttcttt actctcaaag taagagtggt
    13021 ctcacccttc atgggtgagt ggattgaaag atgaacccga cctctctttt aatgaggtcg
    13081 ggtttttaca tgcctttagt caaccattta tctgatagcg tatcaaattc aagtttttgc
    13141 atgttctagt ctatggtttg cgccactagc tttcggtaaa ctaagggtaa cttaagagag
    13201 gagttattta tgacactatc acttgtatta atggtatcgg tttttggggc aggcttgtta
    13261 tcctttttct ctccgtgtat ctttccggtc cttcctgttt atttaggcat tttactggat
    13321 gcagatgatt caaagacgat tactatattt ggtaaaaaac tctattggta tggcattgtc
    13381 aaaaccttag cctttatttt tggtctatct actatttttg tgattttagg ttacggggct
    13441 ggttttttag gaaatatcct ttatgcggta tggtttcggt acttactggg agccttggtc
    13501 attatcttgg ggattcatca gatgggcctc attaccatta agagcttaca atttcaaaaa
    13561 tcactgactt tccataataa caagaatcgc aacggtttgt tcaatgcatt tatccttggt
    13621 ttaaccttta gctttggttg gacgccttgt gtgggacctg ttttgagttc tgttctagct
    13681 ttggtggctt cagggggaaa tggtgcctgg caaggtggcg ttttgatgat tatttatact
    13741 cttggattgg gcattccttt cctactcatc tcttttgcgt caggcattgt tttgaaacag
    13801 tttaacaagc tcaaacccca catattttta ctgaaaaaag taggaggcgt tctgattata
    13861 gtcatgggaa tcttgcttat gacaggaacc ttaaataact tagcacaact ttttggataa
    13921 aggagaaaca caatgaaaaa aggactatta gtaacaactg gtttggcttg tctcgggcta
    13981 ctaactgctt gctcaaccca agacaatatg gttaaaaagg aaataactca ggacaagatg
    14041 agcatggcag ttaaaaagaa agataagatg tcaacatcaa aggacaagtc catgatggca
    14101 gataaatcat ctgataagaa aatgaccaat gatggtccta tggcgcctga tttcgaactc
    14161 aaaggcatcg atggtaaaac ctatcgctta tcagaattca aaggtaaaaa agtttatttg
    14221 aaattttggg cctcttggtg ttcaatctgt ttatcaacct tggcagatac cgaagacctg
    14281 gctaaaatgt cagataaaga ctatgtggtc ttaaccgtcg tctcgccagg tcatcaagga
    14341 gaaaaatcag aagcagactt tacaaaatgg ttccaaggaa cagactataa ggacttacca
    14401 gtcttgttag atccggatgg caagctattg gaagcttacg gcgtcagatc ttacccaaca
    14461 gaagttttca ttggaagtga tggtgttctt gctaaaaaac acattggtta tgccaaaaaa
    14521 tcagacatca aaaagaccct taaaggtata cattaggaag gaccagctga taggcattag
    14581 aaaaaaggag ttgagtttat ggaacacaaa tggaaattat ggctatttat cgttggagtg
    14641 gtcttgttaa taggcggagc ttctgttctc attcagaaag gtcaagccaa ggcttttagt
    14701 caaggcaagc ctcaactacc agctaaaaaa gtcaaatcca ctaaagtaga tcccaatgcc
    14761 cctgttgatc aaaaagtgat ttacctggct ggaggctgtt tctggggcgt tgaagaatat
    14821 ttttcacaag tggatggcgt tctagatgcg gtgtcaggct atgccaatgg tagaggcgat
    14881 accaccaact accagttaat tcatcagact ggtcatgcag aaaccgttga agtgacctat
    14941 gatgcgaacc gtattagctt gaaagaatta ttactgcatt ttttccgaat tattgaccca
    15001 accagtctta ataagcaagg aaatgatcgc ggtagtcaat accgaactgg gatttactat
    15061 actgacaaag cagatttagc tattattgat gaggtcttca aagaaaaagc caaggattac
    15121 aagaaaaaaa tagtggttga aaaagcacca ttgaaacact ttattaaggc agaagactac
    15181 caccaagatt acctcaagaa aaatccaaat ggctactgcc atattgacat caaccaagcg
    15241 acttatcctg tgattgatga aagcaagtac ccaaaaccaa gtgccactga gatcaaagca
    15301 aaactatctg cagacgaata ccgagtgacg caaaaaaacg aaactgaaaa agccttttct
    15361 aatcgctact gggattcttt tgatgcagga atttacgtag acgttgtgac aggagaaccg
    15421 cttttctctt caaaagacaa atttgaatca ggttgtggtt ggccaagttt tagccgcccc
    15481 atcagcccag acgttgtccg ctacaaggaa gataagagtt tcaatatgac gagaacagaa
    15541 gtgcgcagcc gttcagggaa ttctcatctg gggcatgtct ttactgacgg acctaaagac
    15601 caaggcggcc ttcgttactg catcaatagc ttgtctatta cctttattcc aaaagctgat
    15661 atggaagcta agggctatgg ttatttatta tcatctgtag aatagggtta ggcctaaaaa
    15721 tctgatataa tagaagaaag actagtatga gagggccatg caaggccctt tataaagata
    15781 aggagacacc gtgtgtactc attgttaatt gtagaagacg aataccttgt gcgccagggt
    15841 attcgttctt tggttgattt tagccagttc aagattgata gggtcaacga agcagaaaat
    15901 ggccagttgg cttgggactt gtttcagaaa gagccttatg atattgtttt gacggatatc
    15961 aatatgccca aattaaatgg gattcaacta gcagaactca ttaaacagga atccccccaa
    16021 actcatctgg tatttttgac gggctacgat gattttaact atgccttatc tgctttgaag
    16081 ttaggggcag atgattactt gctcaaaccc ttttccaagg ccgatgtgga agatatgtta
    16141 ggaaagctcc agcaaaaatt agacctttcc aagaaaacag aaaccatcca ggaattggtt
    16201 gagcagcctc aaaaagaagt atcagcaata gcaatggcta ttcatgagcg attggcagat
    16261 tctgatttga ccctaaaaag cttggctcag cagcttggtt ttagccctaa ttaccttagc
    16321 gtcttgatta aaaaagattt agggatgccc tttcaagatt atctggtaca agaacggttg
    16381 aaaaaagcca agcttctctt gttaaccagt aatcttaaaa tctatgaaat tgcagagcag
    16441 gtcggttttg aggacatgaa ttatttttct cagcgcttta agcaactggt aggcgttacc
    16501 ccaagtcagt ataaaaaagg aggccaggca tgaagcgtta tcccctactg gtccagttga
    16561 tttcgtatgt ttttgtgatc gtaattgccc tcattaccac gcttggtttg ctttattacc
    16621 agacgagttc tcgtaatatc aggcaactaa ttgaacgtga tacccggcag agcattcggc
    16681 aaagttccca atttattgac gcttacatca agcctcttaa agaaacaact tcggtgctgg
    16741 cgaaaaatac ggaaattcaa gcctttgcta gtcaaattca tcaagaaaat gacaaacagg
    16801 ttcttcagct catgaagatg gttcttgcga ccaattccga tttacaagca gctgttctgg
    16861 tgactaagga tggtcgaacg gtgtctacca attctcagtt gaccatgaaa acctctagtg
    16921 acatgatggc agaaccctgg tataaagcag ccatagaccg tcaggccatg ccagtcttga
    16981 ccccagctcg gcaattatcc ctttcttcta aaaaagaatg ggtagtttct gtgacccaag
    17041 aggtagtaga tagggctggg cataatttag gtgtgctaag acttgatatt gcatacccga
    17101 ccattaaagc gtctttagat cagcttcagc taggccgcca aggctttgcc tttattgtga
    17161 atgataagca tgaatttgtt taccatccca aaaagagtgt ttacagttct tctaaggaga
    17221 tggctgcgat gaagccttat ttagcgattc agaatggtta cactaaggac aagacatctt
    17281 ttgtttacca aaaactcatt cctaacagtc aatggacttt agtgggagtg gcgtcactgg
    17341 atcagttgca ccgggtgcag cgccaaattt tttggtcctt tcttggaaca gggcttctag
    17401 cctgtctgat ttgtggcttt gcaactgtct tagccttacg cagatggatt cgtcccattc
    17461 aacaattgca gcaagttatt cttgctattc aaaaaggaga tcgtcagtta cgtgcccaag
    17521 aaacgggttc tccagaattg acaaaccttg cccaacagtt taatgcgctc ttagatcaaa
    17581 ttgatagctt gatggttgcc gttgcggata aggaaaaggc gattgggcag tataggttac
    17641 aagccttggc tagtcagatt aacccgcatt ttctctataa caccttggac actattattt
    17701 ggatggcaga atttaatgac agcaagcgcg tggtagaagt gaccaagtct ctagctaagt
    17761 attttcgttt ggcccttaat caggggaacg aatacattcg tttggcagat gaactggatc
    17821 acgttagcca atacctcttt attcaaaaac agcgctatgg agacaagcta agttatgaag
    17881 tgcaaggctt agatgtctac gcagactttg ttattcctaa gcttatctta cagcccttag
    17941 tagaaaatgc tatctaccat ggcatcaaag aagtcgatcg caaaggcatg atcaaggtta
    18001 cggtatctga tacagctcag catctggtgt tgactgtttg ggataatggt aaaggcattg
    18061 aagactcttc actgaccaat agtcagagct tgttggctag gggaggtgtg ggccttaaaa
    18121 atgttgacca gcggttaaaa cttcactatg gtgaaggcta ccacatgacc attcatagcc
    18181 agtcagacca gttcactgaa atacaattaa cccttcctaa aatgcatgaa ttaatggcag
    18241 acgacacaca ggaaaatgag taaggttaca ggaagtgcat gtcagccatt tccctaagtt
    18301 agggtattgg agataagaac cttagaaagg atatcatgag tttagcatct gactatttta
    18361 gccgacagac ccccatagtt gaaaagctaa tggcttacgg ttttgaaaaa cgaaataatg
    18421 gttacttcta taacgaacgg tttatggaag aagaatttga ggcgcagctt aggattgatg
    18481 aggctggcaa tatctgggat cgggttattg attgtgactt ggaggaggat tatttgcctt
    18541 tacaacaagc agcctggcaa ggaacctata ctgggcaggt tagagcagcc tatttggaac
    18601 tcttggagcg cttatcagta gcctgttttg aagtcactcc ttttcagtca atgcaagcta
    18661 atagacttgc taagcatatc accaaagaat ggtctgaccc aatggactat ccttttgaaa
    18721 agcacccaga ccttgcaacc tatcgggtag agggcaaatg gtatgctatg attttttctc
    18781 tcttggctga taaattagac cagataccag agagattagt aggacagaca tgtgaagtga
    18841 tgactgtcaa agttaatccc aaggctttcc ctcagttact gcagcaagaa ggcatttacc
    18901 cagcgtatca catgtcaaag aaaaactgga ttagtattat tttggatgat aaggtaacag
    18961 atgataagct ttggacgtta gtaactcaaa gtcgtcaact agtcaaccca aacggtcttt
    19021 ctaatcctaa tggtcctgat tattgggtta ttccagctaa tttgaaatac tatgatattg
    19081 atgcagaatt tgcagccaat gatgtgattt tatggaccca aaaagctggt attgccgtgg
    19141 gagactatgt gctgatttat ataacagccc cagtcaaatc cattcgctat gtctgccaag
    19201 ttctagaaac caatatacct aacgaaggct atcgtaaaaa tcctaacatt gacaaactca
    19261 tgcgtctccg aaaacgccaa cagtacaagg atggtctctt gtcttttgac cttatgaaaa
    19321 aacatggcgt agctgcagtg agagggccaa gacgattaag ccctcaatta attgcatttt
    19381 taaaggaaaa agagtatttt aaagagaaca actaaaggag aataagagat ggttgaattt
    19441 gtagcagatc aaagtttttg ggaacttttt ccagatgcca aactaggtgt cgtcttatta
    19501 aaggatgttc agaatcaagc agagtcacca gaagatgtca aacagctctt agcagacagc
    19561 catgagttag caaaagctta tttaacagca gacaatttta gtgataatca ggtggttcaa
    19621 gtctaccgca aggcctatca acactttaaa accaaaaaag gagctcgctc tagcattgaa
    19681 gcccttctca agcgggtgtc aaatggtcaa agcattccgt cgatcaatcc gctagtggat
    19741 atttataatg cagctagtct acgttttggt ttacctgttg gagccgaaga tagtgacagt
    19801 tttatcggag atttgcgtct gacgataaca gacggtggtg acgactttta cttgattggg
    19861 gatgctgaca ataacccaac cttgccaaat gagctgtgtt ataaagatga tattggagcg
    19921 gtttgtcgtt gccttaactg gcgagatggc gaacgcacca tggtgacaga acacaccaaa
    19981 aatgcatttt tgattatcga agctcttgat caagaaggtc aaaatagatt gcaagaagct
    20041 cttgacttca ttgaaggcag cgcaaaaatg taccttcatg caatcacctc tgtgcatgtg
    20101 ttggataaag acaatccaca cgtgccactt tttacggaaa actagattag taggatgaac
    20161 tgtgtaaaat agaaggttaa caaaaaagcg agtaaacgaa tgtcctgtca tttaggtttt
    20221 tgtttactcg ctttttgatg agattttact gctgtcctag caaaaggtga ggatgaggtc
    20281 gtatatcaca tagaggtttt ctgttaagcc ttatttgtta ctaaagaaga atggtggtgt
    20341 gtaatgacta agggtctcgt aacaagcctt ggcttgctca ttgtcttcac agacaatcaa
    20401 gcaagtatca tcaccacaga tcgttgctac aatttctgga atttgcatgg catctaaaat
    20461 ggagccaaag gattgggcta gtccaggtaa cgtttttaaa ataatttgat gttgaactat
    20521 ctttaacatg accaaagcgt cttccatgta aaaacgaaga cgatgttccc agcgggtttg
    20581 tgaaatggct aacgcttcat agtgtgtgtc gtttccggaa gttactttga ccaggttcaa
    20641 ttccttcatg tcacgtgata gggtagcctg agtgatcgtg atgccatttt tttgcaaccg
    20701 ttcttgtaat tcttgctgtg tatgtatagt tgtctcagag atgagggaac gtatgagttg
    20761 gtgacgcgtc tctttcttat tcattgttat aatcttcctt taacgtaatt gataacaatc
    20821 cgagtatagc aaaaagagga gaaggtgtaa agtttttgat gaaccgctta caaaatggct
    20881 tttttatggt aaaataaaga tgatgtttag aaaaaggaga tttgtgtgat tcgaagagaa
    20941 gattatcaat accttagaaa actaaacgat tttcgctact tttctattga acaatttgac
    21001 aaaattgtag ggcagatgga attccgaaaa gctaaaaagg accatatcct tttttttgag
    21061 ggagataaaa gagataagct ttttctagtg acctctggtt atttcaaggt ggagcagtcc
    21121 gatcaatcag gaaccttcat gtatacggat ttcatcagac acggtaccat ttttccttac
    21181 ggtggcctct ttacagatga ttattaccat ttttcagtgg tagccatgac agacgtgact
    21241 tatttttact ttccagtcga tttatttgaa gattattcgc ttgaaaatcg tctacaaatg
    21301 aaacacttat atagtaagat gtctaaactg ctcgagttac atgagttgag ggttcgaaat
    21361 ttgattacat caagtgctag ttctcgtgtg attcagtctc tggcgatttt gctggttgaa
    21421 atgggtaaag acagtgatac cttacctttt caattaacaa caacagatat tgctcaaata
    21481 agtgggacga caagagaaac tgttagccat gtgcttaggg atttgaagaa gcaagaattg
    21541 attaccataa aaggtaagta tttgacttat ctagataaag actatttttt acaatacaca
    21601 aagtgaatta aaggtcagaa cactttattt taaaagtttt tctgacttat tttaatttat
    21661 aagtataaat ttttacacta aaatgtaaaa aacatacaag taaacgaata caaatctata
    21721 aaaataaaaa taacgccccg aaaatgtaag cgattactca aaatagctgt gagaaatacc
    21781 ctatttttat cttttttaag gagtacacta ggaatataag caagagttaa gaatcttgac
    21841 ggtgaattca caaaaaccaa ttagaaagag gtaattctta tgactgctca aacaccaatt
    21901 catgtttatt ctgaaattgg aaaattaaaa aaagtgttgc tccacagacc cggtaaagaa
    21961 attgaaaatc ttatgccaga ctacttggaa cgcttgttgt ttgatgatat tccattctta
    22021 gaggatgccc aaaaagaaca tgatgctttt gcccaagctt tgcgtgacga aggtattgaa
    22081 gtcttgtacc ttgagacctt agctgcagaa tctcttgtca ctccagaaat tcgcgaagct
    22141 ttcatcgatg aatacctaag tgaagctaat atccgtggaa gagctactaa aaaagccatt
    22201 cgtgaattgt tgatggctat cgaagacaat caagaattga ttgaaaaaac catggcagga
    22261 gttcaaaaat ctgaacttcc agagatccca gcatctgaaa aaggcttgac tgacttggtt
    22321 gaatccaatt acccatttgc gatcgaccca atgccaaacc tttatttcac acgggaccca
    22381 ttcgcaacta tcggtacagg tgtttctctt aaccacatgt tctcagaaac acgtaaccgt
    22441 gaaacccttt atggtaaata cattttcact catcacccaa tttatggggg tggcaaagtg
    22501 cctatggttt atgaccgtaa tgaaaccact cgcattgaag gtggggacga acttgttctt
    22561 tcaaaagatg tgcttgcggt tggtatttct caacgtacag atgctgcttc tattgaaaaa
    22621 ttgttggtta acatctttaa acaaaacctt ggcttcaaga aagtattggc ctttgaattt
    22681 gcaaataacc gtaaattcat gcacttagac actgtcttta ccatggttga ctatgacaaa
    22741 tttaccattc acccagaaat tgaaggcgac cttcgtgttt actctgtcac ttacgacaat
    22801 gaagaacttc atatcgttga agaaaaaggt gatttagcag aacttcttgc tgctaacctt
    22861 ggtgttgaaa aagttgacct tatccgttgt ggtggtgaca acttagtagc agcaggtcgt
    22921 gaacaatgga acgatggttc taacaccctt actatcgcac caggtgtggt tgtggtttat
    22981 aaccgtaaca ccattaccaa tgctattctt gaatctaaag gcttgaaatt gatcaagatt
    23041 cacggaagtg aattggttcg cggtcgtggt ggacctcgtt gtatgtcaat gccatttgaa
    23101 cgtgaagata tttaataagc tatggtaaag gtggttatag gtcagaagcc tttttaaagg
    23161 gcagctagtg tttatcttcg cttctgtgtc tttgtccttc aatgaaattt tgtcatgaca
    23221 gataaatttg atgccaatga cgaaacaaga acggtttatg cagtcgttta tgacaatgac
    23281 cagcccgttt caacaggaca atttttagct gaaacgaaaa tagaagcacg attgacacgc
    23341 attgtaacct tagcagatta ttgtggttgc ggttatggtg ccaaagtcac tgaagcgcta
    23401 gaaacttata ccagacgaga aggcttttac caactaacca ttcacagtga actgacagca
    23461 caaacctttt atgaaaacct aggttatcag tcctatggtc ccaagtgttt agaagatggt
    23521 gagtattgtc agtcccttgc aaaaaccatt cttaaatggg agaagaatat ggacatagca
    23581 atgctaattg cgattgttgg tggtctatta ggctgctatc tctatctcac aaaaaataat
    23641 taatcaaaac agattacagt taacactcga aggagacaat agatgacaca agtatttcaa
    23701 ggacgtagct tcctagcaga aaaagatttt acacgcgctg aattagaata ccttattgat
    23761 ttttcagcac atttgaaaga tttgaaaaaa cgtggtgtgc ctcatcacta cttagaaggt
    23821 aaaaacattg ccctcttgtt tgaaaaaaca tcaactcgta cgcgtgcagc ttttacaaca
    23881 gcagccattg acctaggtgc tcacccagaa tacctcggtg ccaatgacat ccaacttggt
    23941 aaaaaagaat caacagaaga cactgctaaa gtattgggtc gtatgtttga tgggattgaa
    24001 ttccgtggct ttagccaacg catggttgaa gaattggcag aattctcagg tgtgccagtt
    24061 tggaatggtt tgactgatga atggcaccca acccaaatgc ttgctgacta tttaacagtg
    24121 aaagaaaact ttggcaaact tgaaggcctt acccttgttt actgtggtga cgggcgtaat
    24181 aacgttgcca attcccttct tgtgactggt gctatccttg gtgttaatgt tcatatcttc
    24241 tcaccaaaag aacttttccc agaagaagag attgtgacat tggctgaagg ctatgctaaa
    24301 gaaagtggcg ctcgtatcct tatcacagaa gatgctgacg aagctgttaa aggcgcagac
    24361 gtgctttaca cagatgtttg ggtatcaatg ggtgaagaag acaaattcaa agaacgtgtt
    24421 gaattgcttc aaccatatca agtcaacatg aaccttgttc aaaaagcagg aaatgacaag
    24481 cttatcttct tgcactgctt gccagctttc catgacacaa ataccgttta tggtaaagac
    24541 gttgctgaaa aattcggcgt gaaagaaatg gaagtcactg atgaagtctt ccgcagcaaa
    24601 tacgctcgtc attttgacca agctgaaaac cgcatgcaca ctatcaaagc ggttatggca
    24661 gcaacccttg gtaacctctt tattccaaaa gtgtaataag gactggtact ccttaactca
    24721 tttcacatat caatgtgacc cctttaattt aacaatttaa ctacttaaaa acgtacttta
    24781 actacatgac aagtcgttct ggttttcgcg tccttgaccg gaactagaac ggcttatttt
    24841 tatgaaaagg taagaggtaa tcattatgac agaagaaaaa aaacggggtt ttagaattcc
    24901 ttcttcttac accgttcttt ttatcattat agccatcatg gcagtgttaa cttggtttat
    24961 tccagctggc gcctacgaaa ccgctaaggg cggtggtgtt atttcaggaa cctacaaaac
    25021 agtagcatcc aatcctcaag gattttttga catcttgatg gctcccgttc gaggcatgtt
    25081 aggtgttgaa ggcactgatg gtgctattca agtgtctttc tttatcttaa tggttggggg
    25141 ctttttagga gttgtcaaca aaacaggtgc tcttgacaca gggattgcgt cagtggttcg
    25201 taaaaacaaa ggtagagaaa aaatgctgat tgctatttta atccctttgt ttgctctagg
    25261 tggaacgacc tatggtatgg gggaagaaac catggccttt tacccacttt taattcctgt
    25321 tatgattgcg gttggttttg acagtattgt tgctgtagcc attattttga ttggttctca
    25381 aattgggtgt ctagcctcca ctattaaccc atttgcaact ggtgtagcag ctgatgctgc
    25441 tggtgtcagc attgcagatg gaatgatttg gcgtgttatc caatgggtta ttcttgtcgg
    25501 catgtctatt tggtttgttt acaattacgc tagtaagatt gaagaagacc caagtaaatc
    25561 attggttgca gacaaggaag aagagcataa ggaactcttc cagttgcaaa attctggaga
    25621 agacttaaac aagcgccaac gaaacgtttt gaccattttt actttgacct ttgtcattat
    25681 gattcttagt ttgattccgt gggaagattt tggcatcaaa ttctttacca atatcaatac
    25741 ttggttaacc acaatgccta tcttaggggg agttattggg aaaaccatgg gagcatttgg
    25801 tacttggtat ttcccagaaa tcaccatgct ctttatcatg atgggtgtct tagttgctat
    25861 tgtttatcgt atgagtgaag aagacttttt tagctctttt ttgactggtg caggtgaatt
    25921 tttaggtgtt gccatgatct gtgctattgc acgcggtatt caagttatca tgaatggtgg
    25981 tatgattaca gccactatct tacacttagg tgaaacaagt ctttctggtt tatcttctca
    26041 agtttttgtg atattagctt atattttcta cctcccaatg tccttcttga ttccatcaac
    26101 atcaggactt gctggggcta caatggggat tatggcaccg cttggacaat tctcaaatgt
    26161 ccctgctcac cttgttatta cagccttcca gtcagcttct ggaatcttaa acatgatttc
    26221 tccaacttca gcaatcgtta tgggagcact tgcgcttggt cgcgttgacc ttggtacttg
    26281 gtggaaattc attggtaaat ttattgtaat ggtgatgctt gtaagcgtgc tattacttgt
    26341 agttgcaaca ttcttttaac agataggagg ttcctatgga atcctatatc acacctaaac
    26401 atcaagaggc ctgtgtagct gccattaaac agatcgtttc ttacccttct gtttgtcatg
    26461 aaggagaaaa tggcacacct tttggacagg ccattcaaga tgttctcgaa gcaaccttag
    26521 acttatgtca aggtcttggt tttcatactt atattgatcc tgaaggttac tatggctatg
    26581 ctgagttagg tgaccaaaaa gaggtcttgg ctattctttg ccatttggac gttgttccag
    26641 aaggagatcg tcagttatgg aagacagatc cttttgactg tgttgaggca gacggctgtt
    26701 tatttggtcg aggcactcaa gacgataaag ggcctactat gatggcgcta tttgccacca
    26761 aagccttgat agatgcaggt gttaccttta ataaacgcat tcgttttatt ttcggaacag
    26821 atgaagaaac tttgtggcgg tgtatgaatc gctacaatga tgtagaagaa caggctacct
    26881 ttgggtttgc tcctgattca agtttccctt taacctatgc cgaaaaaggc ctacttcagg
    26941 caaaattagt ggggaaaggt tctgataaac tgtcacttga aatcggacaa gcctacaatg
    27001 ttgttcctgc aagagcttct tataaaggca ataagcttga ggctttacaa aaagaactag
    27061 ataagcttgg ctttgagtac gtcgtcaaag agggagaatt aacggtttat ggtctcgccc
    27121 aacatactaa agatgctccc gatggcatca acgctatgat taggcttgcc aaggctttag
    27181 tggtccttga acctaacaaa acccttgatt ttcttgctaa tgtcattgat gaagatggca
    27241 gagcacttaa tatttttggc agaatcgaag atgaaccttc aggcaaatta agctttaatg
    27301 ccgcagggct taccttaacc aaagacaaag cagaaattcg cctagatatt cggattccag
    27361 ttttggcaga taaggaaaaa ttagtccaac aattaagcca aaaagcacaa gaatacggcc
    27421 taacctatga agaatttgat tatttggccc cattatatgt gccattagac agtgaattgg
    27481 taacaacctt gttatctgtc tatcggaaaa aaacaggtga tcaaagccct gcccaatcat
    27541 caggaggggc aacctttgct agaacaatga agaattgtgt tgcctttggt gcccttttcc
    27601 ctgatgctgt tcaaacggaa caccaagaaa atgaacatat tgttttagcg gatgcttacc
    27661 gcgctatgga tatttatgcg gaagctattt accgactaac gcgataacaa gatatgagga
    27721 gtaattatga cgaaacaaaa aatcgtagtc gcacttggag gaaatgctat tctttcaaca
    27781 gatgcgtcag caaaagcaca acaagaagcc ttgatttcca catcaaaatc attggttaaa
    27841 ttaattaaag aaggtcatga agtcatcgtg acccacggca atggcccaca agttggtaat
    27901 ctcttattac aacaagcagc ggcagattct gaaaagaatc cggctatgcc tttggacact
    27961 tgtgttgcca tgacagaagg tagcatcggt ttttggttag ttaatgccct tgataatgaa
    28021 ttgcaagccc aaggtattca aaaagaagtg gcagctgttg tcactcaagt gatcgtggat
    28081 gccaaagacc cagcttttga aaacccaaca aaaccaattg gtccatttct taccgaagaa
    28141 gatgccaaaa aacaaatggc ggagtctggt gcaagtttca aagaagatgc aggtcgtggc
    28201 tggcgtaagg ttgtgccatc accaaaacca gttggtatta aagaagccaa tgtcatcaga
    28261 agtctcgttg attctggcgt tgttgtggtt agtgctggtg gtggcggtgt gcctgttgtg
    28321 gaagatgcaa ctagcaagtc cttgactggt gtggaagctg ttattgacaa ggactttgct
    28381 agccaaaccc tttcagaatt ggtggatgca gacctcttta tcgttttgac aggagttgac
    28441 aatgtttaca tcaacttcaa caaacctgac caagccaaat tggaagaagt gactgtctct
    28501 caaatgaaag aatacatcac acaagaccaa tttgcaccag gtagtatgtt accaaaagtg
    28561 gaagctgcca ttgcttttgt tgaaaacaaa ccaaatgcta aagccattat cacatcgcta
    28621 gaaaatattg acaatgtact atcagcaaac gctggaacac aaattatcgc agggtaatca
    28681 agaggatttt tagttctgtt taaaagactc atttaatggt attttctgaa gagtacgact
    28741 gacataaaga gttgatactg tttttaaagg aaaagagtgt gccataataa aaaacaatag
    28801 tagtattaga aaaggctaat tttattgaga attagccttt tcttttagct ttgataggtg
    28861 tataataacc atactatttt ttatttttca aaatattaga ataaacaaaa gtggacaacg
    28921 tataaagaga actgctaagt cgttgagttc attagtccca aaaagattac caaaaatgaa
    28981 ggaggagcta tgaagaaaag ttttattcat caacaagagg agatttcctt tgttaagaat
    29041 acgttcactc agtatttgat tgctaagtta gatgttgttg aagtgcaagg acccatttta
    29101 agtcgggtcg gggacggtat gcaagataat ttgtccggta ctgaaaatcc agtatctgtc
    29161 aatgtcttga aaattcctaa tgcaaccttt gaagttgtgc attcattagc aaaatggaaa
    29221 cgtcacacct tagcccgttt tgggtttaat gaaggtgaag gccttgttgt taatatgaag
    29281 gctctccgac ctgatgagga ttcacttgac caaacccatt ctgtctatgt tgaccaatgg
    29341 gactgggaaa aagtcatccc tgatggcaaa cgtaatctcg cttacctcaa agaaaccgtt
    29401 gaaaccattt acaaggtcat tcggttaact gaattagctg tagaagctcg ttatgatatt
    29461 gaggctgttt tacctaagaa aataaccttc attcataccg aagaattggt agctaaatac
    29521 cctgatttga cgcccaaaga acgtgaaaat gccattacta aagaatttgg agcagtcttt
    29581 ttaattggta ttggcggggt tttacctgac ggtaaaccac acgatggccg cgctcctgac
    29641 tatgatgact ggacaacaga aactgaaaat ggctatcatg ggcttaatgg tgatattttg
    29701 gtttggaatg accaacttgg ttcagccttt gaattatcct caatggggat ccgtgtggat
    29761 gaagaggccc taaaacgtca ggtggaaatg actggtgacc aagaccgctt agcctttgat
    29821 tggcacaaat ctttgttgaa tggacttttc cctctaacga ttggtggagg aatcggtcaa
    29881 tcacgtatgg tcatgttttt gttgcgtaaa aaacatattg gagaagttca gacctctgtt
    29941 tggccccaag aagttcgtga tagctacgat aatattttat aagtaatcac atcattttgc
    30001 taagttttcc acttgtataa gagactttaa ataaatgatc agaaatattg aaaaaagaaa
    30061 tcatttttaa ggtttagagc tagaaatttg ctataataaa tattatgaga gttgtatcag
    30121 gtgaatttgg tgggcgtcct ttaaagacgc tcgatggaaa gataacacgc cctacttcag
    30181 acaaagttag aggtgcgatt tttaatatga taggacctta ctttaatggt ggtcgtgttt
    30241 tagatttgtt tgcaggttca ggtggattag ccattgaggc tgtttctcgt gggatgtctg
    30301 ctgcagtttt ggttgagaag aatcgtaaag cgcaagctat tattcaagac aatattataa
    30361 tgactaaagc tgagaatcgg tttaccttgt taaaaatgga agctgagcga gctattgatt
    30421 gcttgactgg tcgatttgac ttagtctttt tagaccctcc ttatgccaaa gaaacaattg
    30481 tggcaactat tgaagcttta gcagctaaaa acttattgag tgaacaggtt atggttgtct
    30541 gtgagacaga taaaacagtc ttgttgccaa aagagattgc aacgttaggt atttggaaag
    30601 aaaaaattta tggaattagt aaggtaacag tttatgttaa ctaaaatagg actttataca
    30661 gggtcatttg atcctgttac aaacggtcat ctcgatattg ttaaacgggc aagtggccta
    30721 tttgatcaaa tatatgttgg gatttttgat aatcctacca aaaaaagcta ttttaaacta
    30781 gaagttcgta aagcaatgct tactcaagct ctggctgatt ttacaaatgt aattgtagta
    30841 acttcgcacg aacgtttagc tattgatgta gctaaagaat tacgagtaac tcacttgatt
    30901 agaggcttgc gaaatgcaac tgactttgag tatgaagaaa atttggaata ttttaatcac
    30961 cttttagctc caaatattga gaccgtctat ttgatatcaa gaaataaatg gcaggccctt
    31021 agctctagtc gtgtgagaga gctaattcat tttcagtctt ccttagaagg tttggttcct
    31081 cagtcagtta ttgctcaagt ggagaaaatg aatgaaaaga cttaaaaaaa tcaaatggtg
    31141 gttagtgggt ctgctagctt taatctcttt gttgctagcg ttattttttc cgctacctta
    31201 ttatattgaa atgcctggag gcgcttacga tattcggact gtcttacaag tcaatggcaa
    31261 agaagacaaa cgaaaaggag cttaccagtt tgttgcagtg ggcattagtc gtgccagcct
    31321 cgcgcagcta ttatatgctt ggctgacacc gtttactgaa attagtacag cagaagatac
    31381 aacaggcgga tacagcgata ctgatttcct tcgaattaat caattttaca tggaaacatc
    31441 acaaaatgca gctatttatc aagctttatc cttagctgga aaaccagtta cattagatta
    31501 taaaggcgta tatgttttag acgtaaacaa cgaatctact tttaaaggaa cgttacactt
    31561 agcagatact gtaacaggtg taaatggtaa acagtttact agttcagcag aacttattga
    31621 ctatgtttct cacctaaaac taggggatga agttacggtt cagtttacga gtgataataa
    31681 gcctaaaaaa ggagttggcc gtattatcaa actgaaaaat gggaaaaatg ggattggcat
    31741 tgccttgact gatcatacaa gtgtcaattc agaagacaca gtgatcttta gtactaaagg
    31801 agtaggagga cctagtgctg gtctaatgtt tactcttgat atatatgatc aaataactaa
    31861 agaagattta cgcaagggcc gtacaattgc aggtacagga actattggca aggatggcga
    31921 agtaggagat attggtggtg caggtcttaa agtagttgca gcagctgaag ctggtgcaga
    31981 tatatttttt gttccgaata atcctgttga taaggaaatt aaaaaagtta atccaaatgc
    32041 tataagtaat tacgaagaag ccaaacgggc agccaaacga ctaaagacca aaatgaagat
    32101 tgttcctgtt acgactgttc aagaggcact ggtttatctt cgcaaataaa ataactatga
    32161 atgtattata ttcaattata ttcacgctat taactcataa aaaaacggtt gaaccagata
    32221 gacagatcct tgaccagtct tttgggttca atcatttttt acataaaaca tatctaactt
    32281 gacagcgtaa aaaagatgtt ataaaagtct agtggtaaaa aggcaataga gaattgagta
    32341 aactttagtc gattatctgg aaatagagca tactaaaaac acctagtgaa aattgatttt
    32401 tttcactagc tcatccaaga acttgcgcag ataatcatga gtccattcca tgactctatt
    32461 attctggaaa gccttgcaat agacaagcta acgttataac aactaaatta aaaacttgag
    32521 attgaaaaat ccccataaac ttattttaca aggaggtaag gctatggtaa ccattaaaca
    32581 agttgctgaa gaagcaggtg tttctagatc aacagtctcc aggtatattt ctcaaaaagg
    32641 atatgtatct gatgatgcaa ggcataaaat caaagcagcg atagcaaaat tacattacac
    32701 tccaaatgtc ttggcgcagt cattgaagac gaaaaaaaat caactagtcg gtttattatt
    32761 accagacatt tccaatcctt tttttccgag gttagcaaga ggagcagaag aatatttaaa
    32821 agaaaagggg tatcgggtta tgctaggaaa tatttcagac agcgaagctc ttgaagaaga
    32881 atacgtccat gtccttcttc aaagcaatgc tgctggcatt attactaccc atgactttac
    32941 aaagcggtat cctactcttg ctattccagt tgtagtggta gattgtgttg accaagagac
    33001 ccagtacggt gtcttttcag ataatagggc agggggctta ttggcggctc agactgtctg
    33061 gcaagcaggg gcgaaagaag ttctgttaat aagaggtccg ctggataatg ctgaaaacat
    33121 taatgagcgg tttgaagcta gtttttctta ccttcaaaaa caagacgtga ctatgtatgt
    33181 gtgtgatagt cagaattttg attttgaaag tattcagcta gaagctagtt acaacctcaa
    33241 gtgttaccca acaatagata gtattatagc accttctgat atccatgcta ttgcttatat
    33301 tcatgaactg cattctcaag gtaaaaagat tcctcaagat gttcaaataa taggttatga
    33361 tgacatttta atgagtcaat ttatttatcc ttctttatcg actattcatc aatcctctta
    33421 tttgatgggc cgttatgccg ccgaattagt ttatactatt gctagtcaat taactgtgaa
    33481 agcaaatcgg attaaattgc ctgttcacta cgttgagcga gaaacaataa ggaaaagata
    33541 agtaataata ttagtccaga ttttagcagg tatgatgaag atttattggg atttaccttc
    33601 tactttaaaa gatgttatct gtcattactt tcatcgctat cagtcaaaac gcattcttat
    33661 gcaactctta accttaagct taatgtcttt ttgataacat agaggaatgt tacacgatga
    33721 tgacgcaata aagcctaaat atagtaattg tcgtgatttt atagctagtg agtaatgaga
    33781 tagcaagtct ctcatttact tacaaaagtc aaaaaatagt gtataatggt tgagttacag
    33841 ataacactgc aaaaaagata acaagtgtcc ataattatct aagactataa aggcgatata
    33901 gacagatata aaaaagatga cgaatcagtt atcactggac aattaaccga tttagttaca
    33961 actaatggat ttttcagaaa ggtgtcaaat gaaaaaagaa atttcgccag aaatgtacaa
    34021 ctataataaa tttccaggtc ccaaatttat tcattttgaa gagcaagtta aggctgaagg
    34081 cattgatttg ttactcttag aagatgttaa gaacgctttt gatacgacta gctttggtca
    34141 acgttataca gaagttctcc taaagtatga ctatattgtt ggcgattggg gaaatgaaca
    34201 gcttcgtctt aaagggtttt ataaggatag tgatgatatc aagaaaacga atcgcatctc
    34261 acgtttagaa gattatatta aagaattttg caattttggt tgtgcttatt ttgttttaga
    34321 aaatcttcat ccacaagata ttaaatttga agaggagcgc caaccaagac gaaagaaatc
    34381 acctaaatca aaatcaaatc gtcgcaagcc aaactattca aatcagcagc ccgcaacacc
    34441 taagagtaaa tcgaagcggg tatcaaaaga aaagcaacct gaaaaccaag cgtttaccag
    34501 tcaaaaacgt cgaagtaata ctaagcataa agaaaagtca aaacgtaatc agactagtca
    34561 acttaatacc aaaattagtc attttatcat cagaaagaaa gataaataat ccatgaaacc
    34621 atcgatttat agcttaacac gcgatgaact gatcgcgtgg gcagttgaac gcggccaaaa
    34681 acagtttaga gctactcaaa tttgggattg gctctataaa aaaagagttc aatcttttga
    34741 ggagatgacg aatatttcta aagattttgt ttctatttta aatgatagtt tctgtgtcaa
    34801 tcctttaaaa cagcgagttg tacaagagtc agctgatggt acagtaaaat atttatttga
    34861 actgcccgat ggtatgttaa ttgaaacagt tttaatgcgt caacattatg gtcattctgt
    34921 ttgtgtgacg actcaagttg gctgtaacat tggctgtact ttctgtgcta gtggattgat
    34981 caaaaagcaa cgtgatctaa acagtgggga aattacagct caaatcatgt tagtccaaaa
    35041 atattttgac gatagaaagc aaggtgagcg ggttagccat gttgtcgtaa tgggcattgg
    35101 tgaacctttt gataattaca aaaatgtcat gtgctttctt cgcgtcatca acgatgataa
    35161 tggcttagca attggtgccc gtcatattac agtctctacc tcagggttag ctcacaagat
    35221 tcgggatttt gcaaacgagg gagtacaggt taatttagca gtctcgttgc atgcaccaaa
    35281 taatgattta cgttcaagta ttatgcgagt aaatcgctct tttccgctgg aaaagctctt
    35341 ttcagctata gaatactata ttgaaaaaac gaatcgtcgt gtcacttttg aatacattat
    35401 gctaaatgaa gttaacgata gcattaagca agctcaagaa ttagcagact taactaaaac
    35461 tattcgtaag ttatcttatg ttaacttgat tccgtataat cctgtttcag aacatgatca
    35521 gtatagtaga agtcctaaag agcgagttct agcgttttat gatgtgctaa agaaaaatgg
    35581 agttaactgt gtggtgcgtc aagagcacgg aacagatatt gatgcagcct gtggccagtt
    35641 acgttctaaa acgatgaaaa aagatcgcga aaaggttaca gcgacaaaat aaagcccaaa
    35701 aaatatcttt gattgacaat gcttgatgac aaaatattcc tagtcactaa aatgactagg
    35761 ttttttatct ggcctaaaaa tgctctagaa gcacaatcca aaataatcca aaactaagat
    35821 aaggaataaa aggtatgcgc ttgatgccta atagtaagca agcagtaata cctgctaaac
    35881 tagcaagttg aattagccaa atcaacgacg ttagatccaa tactaaagct aagctagcaa
    35941 ggtagaaaaa atcgcctgct ccaattgaaa aaggtcttaa atgagctaat aaacctagta
    36001 gcagtaatat caatgataaa taattgatcg aataaaacag gagtagactt acaaagctaa
    36061 aaagccacaa gataaaaggg tattcttgag aacgccaatc aaataaacta aataataaag
    36121 aaaagagtaa caagtaacag taactagcac taacccaatg taccatacta gcccataaga
    36181 taattattag acttatctct aagctaaaga agattttttg gctagttata aaatggtttt
    36241 ggagctgccg aaaaacgcgt ttggcaaaag ttagttggtc acaaacaatg acagtctgtt
    36301 tacttagctg ttcttgacca ttatcaggaa aataaagtag taaacagtaa aaaagatggg
    36361 tcaataagat gcccgaacat atactaaaaa aagctatcat acttatttat tcgaaaagaa
    36421 atcaaatact tgcaatcttt ccttaatcat tgtagaatat agatataaaa cgataatgat
    36481 tctaattaag tgtctaatca tccaattagg attattccgt tattgtcgaa taggattagg
    36541 tccttaaccc tttcttatgt ttatcacgtt agggttagtt ccccctccat tcttgccctt
    36601 taagctgaaa agtaaggaga tgttattatg acaaacacac tcgttgaaaa tatttatgct
    36661 tcagtaactc acaatatttc taaaaaagaa gcatcaaaaa atgaaaaaac taaagctgtt
    36721 ttaaaccaag ctgtcgctga cttatctgtg gctgcatcta ttgtgcacca agtacactgg
    36781 tatatgcgtg gtccaggttt cttgtacctt cacccaaaaa tggatgaatt acttgatagc
    36841 ttgaatgcta atttagatga aatgagcgag cgtttgatca ctattggcgg agcgccttat
    36901 tctaccctag ctgagttttc taaacactct aaattggacg aagcaaaagg tacttacgat
    36961 aaaacagttg ctcagcattt agcaagattg gtcgaagttt acctttacct cagcagcctt
    37021 taccaagttg gtttggatat taccgatgaa gaaggcgatg ctggaacaaa cgatttgttt
    37081 acagcagcta agacagaggc tgaaaaaacc atttggatgc tccaagcaga gcgtggacaa
    37141 ggcccagcac tctaatggat tatgccttag gataaaagaa gtcaatgata aaattggctt
    37201 cttttttatg gaatcatcga ttcattttca gaaacataga aagcgattcc atacttgtct
    37261 tgaaatttgc cgtagaattt gctaaaatct acttatatga aaacactata tgatgtgcaa
    37321 caattgctaa aaaactttgg catttttgtt taccttggca aacgtctcta tgatattgaa
    37381 atgatgaaga ttgaacttca gcgtctctat gatagcggct tattggataa gagggattac
    37441 ttaaatgcgg agttaatttt gcgacgtgag catagattag aattggaaaa agaaggatag
    37501 acaaatgagt caaaaattac tggggattga tttaggtggt accaccatta aatttggaat
    37561 tttaacagca gcaggagaag tccaagaaaa atgggcaatt gagactaata ttttagaagg
    37621 aggcaaacat attgtccctg atatcatcgc ttccatcaaa catcgcttag acttgtatgg
    37681 tctaagcagt gctgattttg tgggaattgg tatgggatca ccaggagcgg ttgaccgtga
    37741 tactaataca gttactgggg cattcaacct taattggaaa gaaacccaag aagtcggttc
    37801 cgttgttgaa aaagaattgg gcattccttt tgccattgac aatgatgcca atgtggctgc
    37861 ccttggtgaa cgttgggtag gtgctggtga aaataaccca gatgtcgtct tcatgacact
    37921 tggaacaggt gtcggtggag gcattattgc tgatggtaac ttgattcatg gtgttgcagg
    37981 agcaggtggt gaaatcggcc acatgattgt tgagccagaa aatggctttg cttgtacttg
    38041 tggctcacac ggctgtttgg aaacagtagc ttcagcaaca ggagttgtca aagtggcacg
    38101 tttattggca gaagcttatg aaggggattc agccatcaaa gcagctattg acaatggtga
    38161 aggtgttacc agcaaagata tttttatggc ggctgaagca ggggattcct ttgctgattc
    38221 tgttgtggaa aaggttggtt actaccttgg ccttgcttca gcaaatattt ccaatatctt
    38281 gaatccagac tcagtggtta tcggtggggg tgtgtcggca gcaggagaat ttctacgctc
    38341 acgtattgaa aaatactttg tgaccttcac tttcccacaa gtgaggtatt caactaaaat
    38401 taagattgca gagcttggca atgatgctgg aattatcggt gcagctagcc ttgctcgaca
    38461 atttatcaca aaataggagg gggatatgtc tccaatcaca cttatcttat ggttacttct
    38521 tgtcggcatt gttggttatt atacatggaa ctatttctct tttcgaaaaa tggcaaagca
    38581 ggttgataac gagactttta aagacgtgat gcgtcaaggt caattgattg atttacgaga
    38641 gccagcggct tttagagcta agcatatttt aggagctcga aattttccag cgcaacaatt
    38701 tgatgctgct atcaaaggtc ttcgcaagga caaacctgtt cttatttacg aaaatatgcg
    38761 tccccagtac cgtgtgcctg cggtcaaaaa acttaaaaaa gctggctttg aagacgttta
    38821 tgtcttaaaa gacggtattg attattggga tggcaaagtt aaacaaacaa cctagttaag
    38881 agtaacgtgg agttaagggg aataaaggta gtcactgtct caaaaacctt aaatcctttt
    38941 tttgctgtat ccagacttgc tgaaagtctg aaaacattta caattgatta aaaccagttt
    39001 tttaaaacat tttgtgttat acttatctag ttaaaatata tttacttaga ggaacaaatg
    39061 actaacttaa gaaacgatat ccgtaacgta gcgattattg cccacgttga ccacggaaaa
    39121 acaacacttg tagatgaatt attaaaacaa tcccatactc ttgatgagcg taaagagctt
    39181 caagagcgtg ccatggattc caatgacctt gaaaaagaac gtgggattac aatccttgcg
    39241 aaaaatacgg cagtagccta taacgatgtt cgtattaaca tcatggatac cccaggacac
    39301 gcggacttcg gtggtgaagt tgaacgtatc atgaaaatgg ttgacggggt tgttcttgtt
    39361 gtggatgcct acgaaggaac aatgccccag acgcgtttcg tattgaaaaa agcacttgag
    39421 caaaacctta tcccgatcgt tgtggtgaac aagattgaca aaccttcagc tcgtccagca
    39481 gaagttgtag atgaagtgct tgaattattc atcgaacttg gtgccgatga tgagcaattg
    39541 gaattcccag ttgtttacgc atcagctatt aatggaacat catcattatc agatgaccct
    39601 gctgaccaag agcatactat ggcaccgatc tttgatacga ttattgatca tattccagcg
    39661 ccagttgata attcagatga gcctttgcaa ttccaagtgt cacttttgga ctacaacgat
    39721 ttcgtaggtc gtatcggtat cggtcgtgtt ttccgtggta ctgttaaagt gggtgaccaa
    39781 gtaactcttt caaaacttga tggtaccact aaaaacttcc gtgttacaaa actgtttggt
    39841 ttcttcggtt tggaacgtcg tgaaattcaa gaagctaaag caggtgactt gattgctgtt
    39901 tcaggtatgg aagatatctt tgttggagaa accattacac caactgactg tgtggaagct
    39961 ctgccaattc ttcgtattga tgagccaaca cttcagatga ctttcttggt caataactct
    40021 ccttttgcag gtcgtgaagg taaatggatc acgtcacgta aggttgaaga acgtctttta
    40081 gcagaattgc aaacagacgt gtcacttcgt gttgacccaa cagattcgcc agataaatgg
    40141 acggtttcag ggcgtggaga attgcattta tctatcctca ttgaaaccat gcgccgtgaa
    40201 ggctatgaac ttcaagtatc acgtccagaa gttatcatca aagaaattga tggtgtcaaa
    40261 tgtgaaccgt ttgagcgtgt tcaaattgat acaccagaag aatatcaggg tgcaatcatc
    40321 cagtctcttt cagaacgtaa aggggatatg cttgatatgc agatggttgg taatggtcaa
    40381 acgcgtttga ttttcttgat tcctgcacgt ggtttgattg gttattcaac agagtttctt
    40441 tcaatgacac gtggatatgg tatcatgaat catacttttg atcagtatct accggttgtt
    40501 caaggagaaa tcggtggccg tcatcgtggt gccttggttt ctattgaaaa tgggaaagca
    40561 acgacatatt caatcatgcg tattgaagaa cgtggaacta tctttgtcaa cccaggtaca
    40621 gaagtttacg aagggatgat tgttggtgaa aactcacgtg ataacgacct tggtgttaac
    40681 attacaacag ctaagcaaat gacaaatgtc cgttcagcga ctaaagatca aactgcagtt
    40741 atcaagacgc ctcgtatcct aacgcttgaa gaatcattag agttcttgaa tgacgacgaa
    40801 tacatggaag taacgccaga atctatccgc ttgcgtaaac aaatcttgaa taaagctgcg
    40861 cgtgataaag ctaataaaaa gaaaaaatca gccgaatagc ttttgaagtt aaactaacta
    40921 aataaaatag caaaaagata tttaagaaaa gtcatttttt aaaggtcttt ttgcgtttac
    40981 aagttcctat ggaatataaa ccaaaataaa ggaggacgga atgttttacc ttattatggc
    41041 tgtctcgatt atatcttatt atctatacat ggctcctaaa agtgtccgta acacccttgg
    41101 gatgattggt ttagttggct tggttgctct gcttattgtt ttagcgggct tgagttttat
    41161 caaaatcatg caaacccctc ctgaattttt tattgggatg ggaatggtgg cattgggcta
    41221 ttttgcttta aaagatgtgc gtaaaatgac caaaaaaccg agagttaaat aa
//
LOCUS       DATUYR010000011        40054 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.011, whole genome shotgun sequence.
ACCESSION   DATUYR010000011 DATUYR010000000
VERSION     DATUYR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40054
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.011"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            164..1012
                     /locus_tag="VCI33_000505"
     CDS             164..1012
                     /locus_tag="VCI33_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEP2831447.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQALPDDLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQSLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
     gene            1310..2443
                     /gene="ugpC"
                     /locus_tag="VCI33_000506"
     CDS             1310..2443
                     /gene="ugpC"
                     /locus_tag="VCI33_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEP2831448.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            2572..4185
                     /locus_tag="VCI33_000507"
     CDS             2572..4185
                     /locus_tag="VCI33_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEP2831449.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTSGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDSEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAEELDVPALKTIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFAKDLTGAQ
                     VIITNTDVDTVFETKHLQPWDAFCLKLKA"
     gene            4383..7880
                     /locus_tag="VCI33_000508"
     CDS             4383..7880
                     /locus_tag="VCI33_000508"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEP2831450.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGLQSSSKEAKPVLDSSLANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQIFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADL
                     TKSDKGVWRAHLTSDSIKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSVNPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSIAYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYRTKVS
                     DDKVPNKATLIDAVAKYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            7995..8369
                     /locus_tag="VCI33_000509"
     CDS             7995..8369
                     /locus_tag="VCI33_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEP2831451.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            8485..8850
                     /gene="yajC"
                     /locus_tag="VCI33_000510"
     CDS             8485..8850
                     /gene="yajC"
                     /locus_tag="VCI33_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEP2831452.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            9069..9818
                     /locus_tag="VCI33_000511"
     CDS             9069..9818
                     /locus_tag="VCI33_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEP2831453.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            9831..10625
                     /locus_tag="VCI33_000512"
     CDS             9831..10625
                     /locus_tag="VCI33_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEP2831454.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            10698..11957
                     /gene="rseP"
                     /locus_tag="VCI33_000513"
     CDS             10698..11957
                     /gene="rseP"
                     /locus_tag="VCI33_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEP2831455.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLDETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMSDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            12155..14011
                     /locus_tag="VCI33_000514"
     CDS             12155..14011
                     /locus_tag="VCI33_000514"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEP2831456.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPART
                     DLDRWVVLDKSIASMDDIPKEVLEDIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRTVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            14265..18662
                     /locus_tag="VCI33_000515"
     CDS             14265..18662
                     /locus_tag="VCI33_000515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEP2831457.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNSHLPNLVKQFEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHDRKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQDAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLAKYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRANKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVENTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            18772..19200
                     /locus_tag="VCI33_000516"
     CDS             18772..19200
                     /locus_tag="VCI33_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP2831458.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            19331..19873
                     /locus_tag="VCI33_000517"
     CDS             19331..19873
                     /locus_tag="VCI33_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP2831459.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANVPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
     gene            complement(19906..20520)
                     /locus_tag="VCI33_000518"
     CDS             complement(19906..20520)
                     /locus_tag="VCI33_000518"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP2831460.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(20646..20903)
                     /locus_tag="VCI33_000519"
     CDS             complement(20646..20903)
                     /locus_tag="VCI33_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP2831461.1"
                     /translation="MTQNVCQQNITYTVKERFASYTLEAQANQEVHLNLTLLANRFGT
                     SDRHLKHVLKQPIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            21029..21184
                     /locus_tag="VCI33_000520"
     CDS             21029..21184
                     /locus_tag="VCI33_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831462.1"
                     /translation="MTMLGYRLSAEVRGSAILPLLAGYLIRQAIAQVYSDLKTASWLF
                     GLASSVA"
     gene            21342..21611
                     /gene="rpsO"
                     /locus_tag="VCI33_000521"
     CDS             21342..21611
                     /gene="rpsO"
                     /locus_tag="VCI33_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEP2831463.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            21964..24024
                     /locus_tag="VCI33_000522"
     CDS             21964..24024
                     /locus_tag="VCI33_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP2831464.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEVVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKPVNEDEIAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     VKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLFGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            24017..24301
                     /locus_tag="VCI33_000523"
     CDS             24017..24301
                     /locus_tag="VCI33_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2831465.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            24328..25707
                     /locus_tag="VCI33_000524"
     CDS             24328..25707
                     /locus_tag="VCI33_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEP2831466.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLAFARFTRFKYIFLTGHHSFFMACLLSAVLGAVDFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVLFYLVATVASVLRNTSVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            25717..26448
                     /locus_tag="VCI33_000525"
     CDS             25717..26448
                     /locus_tag="VCI33_000525"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEP2831467.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPVIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEEVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDDNH"
     gene            26728..28860
                     /gene="pnp"
                     /locus_tag="VCI33_000526"
     CDS             26728..28860
                     /gene="pnp"
                     /locus_tag="VCI33_000526"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEP2831468.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSILTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            28853..29605
                     /locus_tag="VCI33_000527"
     CDS             28853..29605
                     /locus_tag="VCI33_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEP2831469.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSVALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQESAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            29614..30195
                     /gene="cysE"
                     /locus_tag="VCI33_000528"
     CDS             29614..30195
                     /gene="cysE"
                     /locus_tag="VCI33_000528"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEP2831470.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTVRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            30674..30904
                     /locus_tag="VCI33_000529"
     CDS             30674..30904
                     /locus_tag="VCI33_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831471.1"
                     /translation="MNKIRVGLYVIMSLSILVSSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            30932..32275
                     /locus_tag="VCI33_000530"
     CDS             30932..32275
                     /locus_tag="VCI33_000530"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEP2831472.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFAIADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            32268..32681
                     /locus_tag="VCI33_000531"
     CDS             32268..32681
                     /locus_tag="VCI33_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEP2831473.1"
                     /translation="MINPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            32877..33674
                     /locus_tag="VCI33_000532"
     CDS             32877..33674
                     /locus_tag="VCI33_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831474.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASIRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQTNQSANQEIDSTSNPFKSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLSGLVVSLAAGLFIAIQKVSRRK"
     gene            33721..34467
                     /gene="rlmB"
                     /locus_tag="VCI33_000533"
     CDS             33721..34467
                     /gene="rlmB"
                     /locus_tag="VCI33_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEP2831475.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            34471..34989
                     /locus_tag="VCI33_000534"
     CDS             34471..34989
                     /locus_tag="VCI33_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEP2831476.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKINQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            35086..35946
                     /locus_tag="VCI33_000535"
     CDS             35086..35946
                     /locus_tag="VCI33_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP2831477.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            36082..36780
                     /locus_tag="VCI33_000536"
     CDS             36082..36780
                     /locus_tag="VCI33_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831478.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAVILTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPRYDERQKQILLKGYT
                     IGFWFMIVAVWLSIFLHRFSERLVSASFLFTLALWGGLAVQTTYCNLNGVSPFVDRRF
                     GKQGILVGMIATGMSLITIGLAVREMMAGQLKMGSFFKAGGSGSLMVLGITLLSMGAS
                     IFYRHYLDAKEADE"
     gene            36777..36983
                     /locus_tag="VCI33_000537"
     CDS             36777..36983
                     /locus_tag="VCI33_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2831479.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEVDDSH"
     gene            37201..37647
                     /gene="rplM"
                     /locus_tag="VCI33_000538"
     CDS             37201..37647
                     /gene="rplM"
                     /locus_tag="VCI33_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEP2831480.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            37668..38060
                     /gene="rpsI"
                     /locus_tag="VCI33_000539"
     CDS             37668..38060
                     /gene="rpsI"
                     /locus_tag="VCI33_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEP2831481.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(38193..38342)
                     /locus_tag="VCI33_000540"
     CDS             complement(38193..38342)
                     /locus_tag="VCI33_000540"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831482.1"
                     /translation="MIEIGVDPVNTAKRLGHASSQITLDTYSHATAVGEDKSIKQFAN
                     YLKNN"
     gene            complement(38378..38740)
                     /locus_tag="VCI33_000541"
     CDS             complement(38378..38740)
                     /locus_tag="VCI33_000541"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP2831483.1"
                     /translation="MAALRKGELCALKWANIDFQSETLSVNKSLGRLDGQAIEKSTKN
                     DFSVRKIKLDSETISILQEWKAISQKEKAQLAVAPLSIESDFIFTYCTREGSIEPLHT
                     DYINNILSKVIKQHSLKK"
     gene            complement(38703..39197)
                     /locus_tag="VCI33_000542"
     CDS             complement(38703..39197)
                     /locus_tag="VCI33_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HEP2831484.1"
                     /translation="MYLKASGNLEEFTKPTHKTYSEIFEEWYQAYQDTVEPTTASRTL
                     DLFQLHILPVMGELPINKTSPLDCQNFITDKAKTFKNIKQIKSYTEKIFDFAIKMKLL
                     KHNPMAEIIMPKRKKTRIENYWTVQELQEFLAIVLQKEPYKHYALFRLLAYSGLKERR
                     AMRS"
     gene            complement(39325..39660)
                     /locus_tag="VCI33_000543"
     CDS             complement(39325..39660)
                     /locus_tag="VCI33_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP2831485.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFREVGFDADEKYGSKIIHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     QSDYIKLFK"
     gene            complement(39650..39886)
                     /locus_tag="VCI33_000544"
     CDS             complement(39650..39886)
                     /locus_tag="VCI33_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831486.1"
                     /translation="MVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKE
                     KLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
BASE COUNT        11787 a         7545 c         8925 g        11797 t
ORIGIN      
        1 aatagctatt tgattgaatg ttcttgctaa aaatgaataa cacttattta gctttaatga
       61 ttaaaaagac agaaaataca aaatcgtgct gacaacaaac cgaactgtgt gataaagagt
      121 catgtgttat aatggtttaa agaccatcat gaaaggaatg gctatggagc taaaagacta
      181 ttttccagag atgcaagtag gccctcatcc gttaggagat aaagagtggg tgagtgtcaa
      241 agaaggggac caatatgtgc attttcccaa atcatgttta tccgaaaaag agcgcttatt
      301 acttgaggtg gggctgggtc agtgtgaggt cttgcagccg ctagggtcac cttggcagcg
      361 gtatttgctt gatcatcagg gcaatccccc tcagctgttt gagacttctc agttcattta
      421 cctcaatcat cagcaggctt taccagatga cttagttgag ttgctccaac agatgattgc
      481 agggcttgag gtcattttgc ctatcagtgc aactcagacg gcatttttat gccgacaagc
      541 aatctcaata aaggtcttgc gctggcttga agatctcctg ccaacgctag aaagtgattt
      601 tggtttggcc ttaaccatgt ttgttggaaa tgcctggtat caagtagctg cgggtacatt
      661 gagagagtgt tttgaggagg aatgtcagct gttaacggcc tatttaaggc aacaatcagg
      721 cagaaaatta ttaacatttt caggactgat gctgtggtct ttgctgtcgc accacacttt
      781 tttggcattg acgcgtcagt ttcaccaatc cctaagtcct caaagtgata tggccgatgt
      841 ggttcacgcg ctatggtcag aacacggcaa tctggtgcag acagcgcagc gcttgtatat
      901 tcaccgtaac tccttgcaat acaaacttga taaatttgct cagcaatcag gactccactt
      961 gaagcaattg gatgatttag cctttgctca tctctttttg ttgaaatatt aggaaagcca
     1021 cagagctttc ttttttgctg cctggactgt agctgataag gagaatgata gatcgttcag
     1081 cttgttttgg ctcctgtgac cggcctgtgt ttacatagtt ttcttggttt atggtccctt
     1141 acgacttgac tagccctgct attgtgcaag ttgcccaaag aagtttggtc gctttgtctc
     1201 tgttttgaaa gcgctatttt tgttataata aaagcatcat ataaaagata tcagcggttt
     1261 ctagctttgc agtcagctag aacatttgct ggctaaatag gagttataca tggttgaatt
     1321 aaatctaaat cacatctata aaaaataccc aaatactact cattatgccg ttgaagattt
     1381 tgacttggac attaaagaca aagaatttat cgtctttgtt ggaccttcag gatgtggtaa
     1441 atcaaccact cttcgtatga ttgcaggtct tgaggacatt tctgaaggag agttaaaaat
     1501 cggtggcgaa gtggtgaatg acaagtctcc taaagaccgt gatattgcca tggtctttca
     1561 aaactacgcc ctttacccac acatgaccgt gtatgataac atggcctttg gtttgaaatt
     1621 acgtaagtac aaaaaagatg atattgaccg tcgtgtcaag gaagctgcgc agattcttgg
     1681 tttgaccgaa tttttagagc gtaaacctgc tgacctgtct ggtggtcaac gtcagcgtgt
     1741 ggctatggga cgtgctattg tccgtgatgc taaagtgttc ttgatggacg agcctttgtc
     1801 aaacttggat gcgaagcttc gtgtctccat gcgtgctgaa attgctaaaa ttcaccgtcg
     1861 tatcggctca acaactattt acgttaccca tgaccagaca gaagccatga ctttggcaga
     1921 tcgtatcgtt atcatgagtg ccactaaaaa ccctcaaggt aatggaacga ttggtaaaat
     1981 tgagcaagtg ggttcaccac aagaattgta taaccttcca gctaataaat tcgttgccgg
     2041 ttttatcgga agccctgcca tgaacttctt tgaagtggaa gttaaagatg gccgcattgt
     2101 gagtgaagat ggtcttgcta ttgcgattcc tgaaggtcaa gcaaaaatgc ttgaagcggc
     2161 tggttacaag gggaaaaaag tcacttttgg tattcgtcca gaagacattt ctagtagaca
     2221 aatcgtgcat gatacttatc caagtgcaac tgtcacagca gaagttttgg tttctgagtt
     2281 actgggctct gagacgatgc tctatgtcaa gcttggacaa actgagtttg cttctcgtgt
     2341 agatgcgcgt gatttccata gtccagggga gcaagttagc ttgaccttta atgtggctaa
     2401 agggcatttc tttgacagag ataccgaaca agctattcgt taaggtgtaa gcgatattca
     2461 taaaaaggtt gggttccaac ctttttatca tgtcatttgc gcaaacgatt gcacaaaaag
     2521 agaaaaaacg ttaaaataaa agatgaatga atgattaata ggagaagaat aatgaagaaa
     2581 cactggtggc ataaagctac gatttatcag atttacccga gatcctttaa agacaccagc
     2641 ggcaatggta ttggtgattt aaaaggcatt accagtcaac tggactactt gcaaaaactt
     2701 ggcattacag ccatttggtt atctcctgtt taccaaagtc caatggatga caatggttat
     2761 gacatctctg actatgaagc gattgctgat gtttttggag acatggcaga tatggatgag
     2821 ctcttagcag cagctaatga gcgaggcatt aagattatca tggacttggt ggtcaaccat
     2881 acgtctgatg agcatgcttg gtttgttgaa gctagggaaa accctaatag tcctgagcgt
     2941 gatttttata tttggcgaga tgaacctaac gatttgactt ctatttttag tggctcagct
     3001 tgggaatatg ataatgcatc aggccaatat tatttgcacc tttttagcaa acgtcagcca
     3061 gatctcaact gggagaatga agctttacgt cataaaattt acgatatgat gaatttctgg
     3121 attgctaaag gcatcggtgg ttttcgtatg gacgtgattg acctgattgg gaaggttcca
     3181 gattctgaaa tcacgggtaa cgggccgcgc ttacacgatt acctcaaaga aatgaatcaa
     3241 gcaacctttg gcaatcatga cgtgatgact gtcggtgaga cttggggagc aacgcctgaa
     3301 attgctcgcc agtattctcg ccctgaaaac aaagagttat ccatggtctt ccaatttgag
     3361 catgtgggtc ttcaacataa acctgacgct cccaagtggg attatgcgga agaactggat
     3421 gttcctgctc ttaaaactat tttcagcaag tggcagacag aattaaagtt gggagaaggc
     3481 tggaattcgc ttttttggaa taaccatgac ttgccacggg tcttatccat ttggggcaat
     3541 gacagcactt accgagaaaa atcagccaag gctttagcta ttttactgca tttgatgcga
     3601 ggaacccctt acatttacca aggagaagag attggcatga ccaattatcc ttttaaagac
     3661 ttaaccgagg ttaatgatat tgagtccctt aactatgcta aggaagctat gggaaatgga
     3721 gtgtcagctg cgcgtgtgat ggatagcatc cgtaaggtag gacgagacaa tgctagaacg
     3781 ccgatgcagt ggtctaagga cacacatgct ggcttttctg aagcaaaaga gacttggttg
     3841 ccggtcaacc ccaactacca agacatcaat gtggccgatg cccttgctga tccagactct
     3901 attttttaca cctaccaaaa attgattgcc ttgcgtaagg agcaggactg gctagttgaa
     3961 gcggattatc acctcttgcc aacagctgat aaagtgtttg cttatcagcg tcagcttgga
     4021 gaagagacgt atgtgattgt tgtcaatgtg tctgatgagg agcaagtctt tgccaaagac
     4081 ttaacaggtg cacaagtgat catcaccaat acagatgtgg acacggtgtt cgagaccaag
     4141 cacttacaac cttgggatgc cttttgtctc aaactgaagg cctagcagtt gtatgtgatt
     4201 tgagtttacc caacagccaa gcgagtggaa actcgttttg gctgtttgat ttttgagatc
     4261 ctattaaggg gtgatagtca ttgtttgagc tgtgttctaa gttgaagtgc ggcatgtctt
     4321 gcaagcgctt gcgcaatgtg ataagatagt cttgtttgaa tgttatacta aatggaggtc
     4381 ttatgaaaaa gaaagtcaac caaggatcaa agcgctatca atatctgtta aaaaagtggg
     4441 ggataggttt tgtaatcgct gcaactggga ctgtcgtgtt agggtgcacc cctagtatct
     4501 taacacatca agttgctgct aaaaccattg ttggactagc ccgcgatgaa gctcaacaag
     4561 gagatggcaa tgctaaatct ggtgatggtc ttcaatcgtc tagcaaggag gcaaaaccag
     4621 ttttagacag ctcgttagct aatcctgcta gtattgctga gcatcatttg cgtatgcatt
     4681 ttaaaacatt gccagctggt gagtcgctag gaagcttggg actttgggtg tggggagatg
     4741 tggatcaacc ttcaaaggat tggccaaatg gtgctatcac catgacaaaa gcgaaaaaag
     4801 atgactatgg ctattatcta gatgtgccac tagcagctaa acaccgccag caagtgtctt
     4861 atctcattaa taataaagct ggagagaatc tttcaaagga ccagcacatc tcgcttctca
     4921 caccaaaaat gaatgaagtt tggatagacg agaattacca tgcgcacgct tatcgacctt
     4981 tgaaaaaagg ttaccttcga atcaactacc acaatcaatc gggacactac gataacttag
     5041 ctgtctggac ctttaaagat gtcaaaaccc caacgaccga ctggccaaat ggacttgact
     5101 tgtcacataa agggccttat ggagcttatg ttgatgtccc cttaaaagaa ggagctaacg
     5161 aaatcggatt tttaatcctt gataaaagta agacaggaga tgctattaaa gtgcaaccaa
     5221 aagattatct gtttaaagag ttagacaatc atactcagat ttttgtcaaa gacactgacc
     5281 caaaagtcta caacaatcct tattatattg atcaggttag tctcaaagga gctgaacaaa
     5341 ccacgccaaa tgagattaaa gccattttta cgaccttaga tgggcttgat gaagatgcgg
     5401 tgaaacaaaa catcaagatc actgacaaag cagggaaaac tgttgcaatt gatgagttga
     5461 cacttgacag ggataagtct gtaatgacat taaagggtga ttttaaggcg caaggtgcag
     5521 tctacacggt tacatttgga gaagttagcc aagtcgctcg ccaatcctgg caattaaaag
     5581 ataaactcta tgcttacgat ggtgaacttg gagctaccct agctaaggat ggttctgttg
     5641 atttagcgct atggtctcca agtgctgata ctgttaaggt tgtcgtttat gataaacaag
     5701 atcagacaag ggtggttggt caagctgatt tgaccaagtc ggacaagggt gtttggagag
     5761 ctcatctgac ttctgatagt atcaagggca ttagtgatta cacaggctac tattaccttt
     5821 atgaaatcac gcgcggtcag gaaaaagtca tggttttgga tccttacgcc aaatctctcg
     5881 ctgcctggaa tgatgcgact gctactgatg acatcaaaac agcaaaagct gcctttattg
     5941 atccaagcaa actaggacca acaggccttg attttgccaa aattaacaac tttaaaaagc
     6001 gtgaagacgc tattatctat gaagcacatg tgcgagattt tacgtcagat aaggctctag
     6061 aaggcaagtt aacacaccct tttgggactt tttcagcttt cgttgaacag ctagactatc
     6121 tcaaagactt gggggttacc cacgttcaat tgctaccggt tttgagttat ttttatgcca
     6181 atgagctgga caagagccgc tcaacagcct acacgtcttc agacaataat tacaactggg
     6241 gttatgaccc acaacactac tttgcccttt ctggcatgta ttcggtaaat cctaacgacc
     6301 ctgctttacg tatcgcagag cttaaaaacc ttgtcaatga gattcacaaa cgtggtatgg
     6361 gtgttatttt tgatgtggtt tataaccaca cggctagaac ctatctcttt gaagatttgg
     6421 aacccaacta ctatcatttt atgaatgctg atggcacagc tagagagagt tttggcggag
     6481 gtcgtctagg aacgacacat gccatgagtc gtcgtatctt ggtggattcg attgcttatc
     6541 tgactcgtga attcaaggta gatggttttc gtttcgacat gatgggtgac catgatgcgg
     6601 cagctattga gcaagccttt aaggcagcca aagccattaa tccaaatacc attatgattg
     6661 gcgaaggctg gcgtacctac caaggtgatg aggggaaaaa agaaattgcg gcagatcaag
     6721 attggatgaa agcaaccaat acggtcggtg ttttctctga tgatatcaga aataccctca
     6781 agtcaggttt cccaaatgaa ggcacagcag cctttattac tggtggcgca aaaaatctag
     6841 aaggactgtt caaaacgatc aaagcacagc ctagtaactt tgaagcagat gccccaggag
     6901 atgtagtgca gtatattgca gcccatgaca acctgacctt acatgatgtc attgccaaat
     6961 ccatcaataa ggatcctaaa gtggctgaag aagagattca taagcgtatt cgtctaggaa
     7021 ataccatgat tttaactgct caagggactg cctttatcca ttctggtcag gaatatggac
     7081 gaaccaagca gcttctaaat cccgactaca ggacaaaggt gtctgatgac aaggtgccaa
     7141 ataaggcgac tctgattgat gctgtagcga aataccctta cttcatccac gattcttatg
     7201 attcgtctga tgcggtcaat cattttgact gggcaaaggc aacagattcc atagctcacc
     7261 cgattagcaa ccaaacaaaa gcctatacac agggcctaat tgcgttgcgt cgctcaacag
     7321 atgcctttac aaaagcaacc aaagctgagg tagatcggga tgtgaccttg atcacccaag
     7381 caggacaaga tggtattcaa caagaggacc ttatcatggg ttaccaaacg gtggcatcaa
     7441 atggagatcg ctatgctgtc tttgtcaatg cagacaacaa gacccgcaag gtagttttac
     7501 ctcaagccta ccgctatttg ctaggagccc aagtgcttgc tgatgctgag caggctggtg
     7561 ttactgccat tgctaagcct aagggagtcc agtttaccaa agaaggcttg actattgatg
     7621 gcctaactgc cctggtcctc aaagtatcct caaaaacagc taatccctct cagcaaaaga
     7681 gtcagacaga caatcatcaa accaaaacac cagatggctc aaaagaccta gataaatcat
     7741 taatgactag gccaaaaaga gctaaaacaa accaaaagct cccaaaaacg ggtgaagcct
     7801 cctcaaaagg cttattagca gctggagtag ctctgctttt attggctatt agcctgttga
     7861 tgaagcgcca aaaagattag ggtggtgtta atagagacaa aaagattgct gtttgcggat
     7921 tctttttgac ggctataaaa gctctgagct gtgaaaagac cttgtgattg agaaaattgc
     7981 tactttagtg tatcatgaga ccaaaagaga aggagacatc catgacattt gaagaaatcg
     8041 ttgcgaattt tataccgtcg agtgttgcag aagtcacatc agctattgct agtggcaaag
     8101 acatgattgt ctttttagga cgttcgagct gtccttattg tcgccgcttt gctcctaagt
     8161 tggcacaagt ggcaacagat aatcaaaaag aagtctattt tgtggatagt gaaaacgctg
     8221 ctgatgctgc tgagcttgct gcctttcgtg agaactacca attagtgaca gttccggctt
     8281 tgctggttag ttacgatcag caccaaaggg ctgtatgtga ttcaagtttg acaccagatg
     8341 acatcttagc ttttttgact cgtgagtaaa ggactgcgct tagcgtctcg tttaaagaag
     8401 aaatcaggta acgtctagtc ttgctatacc tcttgatttt tgatatagta agacatgact
     8461 tttatagaat aaaaaggaga cattatggga tttccaacaa ttttaatgtt tgtagtgatg
     8521 cttggattga tttggtttat gcaacgtcaa caaaaaaaac aagcgcaaga acgccaaaac
     8581 cagttaaacg ctattgaaaa aggagacgaa gtcgtaacga ttggtggcat gtttgcgatt
     8641 gttgacgaag tcgatacaac agctaaaaag attgtgttag atgtggatgg cgtctttttg
     8701 acatttgagc tatcagctat taagcgtatt gtaacaaaag ctaccacaga aacgacactg
     8761 gttgaaacag aagaaaccag tgttgttaat gagcctgctg ttgagacagt agatagcact
     8821 gacactgaca gtgccattga aagccattaa ggcggggaaa gtttggatga aatatgccaa
     8881 acttttttgt tttgatccct ggactgaaac agttattctg atgctaacag gttcatgaaa
     8941 agctgacaca tctatcagag gataggtgaa cattttgtga agataagatc taattgataa
     9001 aaaacaagcc aaaaggcgtg aaatatgata aaatatgaga taagtatgct tatcaataag
     9061 gaaaaaacat gtttggatta aaagcgaaat caacaaaaaa agttttgggg agtatcccaa
     9121 agcatattgg aattatcatg gatggtaatg gacgttgggc caaaaaaaga ctaaaaccta
     9181 gagtgtttgg gcataaagct gggatggatg ctttacaaga agtaacgatt actgcatctg
     9241 agttgggggt taaagttctg acagtctatg ccttttcgac agaaaactgg tccagacccc
     9301 aagatgaggt gtcttttatc atgaatttac cagtaacctt ctttgacaaa tatgtgcctg
     9361 tgcttcatga gaacaatgta aagattcaaa tgattggaga gactagtcgt ttacctgaag
     9421 ataccttggc agcgctaaat gcagccatcg acaaaacaaa acgtaatacg ggtttaattt
     9481 tgaattttgc tctcaattat ggaggacgtg ctgagattac cagtgctgtt cgctttattg
     9541 ctcaagatgt cttagatgcc aaattaaatc caggagatat tacggaggat ttgattgcta
     9601 attacttgat gacagaccat ctgccatatc tctaccgaga ccccgatttg attattcgca
     9661 ccagtggtga gctgcgtttg agcaatttct tgccttggca atcagcttac agtgaatttt
     9721 atttcacacc agtcttgtgg cctgatttta aaaaggcaga gctactaaag gccattgcgg
     9781 attataaccg ccgccaacgt cgttttggca aggtctaaaa ggagatagtt atgaaagaac
     9841 gtgttgtttg ggggggagta gcagtagcta ttttcctacc ttttctcata ataggaaacc
     9901 taccattcca attatttgta ggcgttttgg ccatgattgg ggtatcagaa ttactgaaaa
     9961 tgaaaagatt agaagtcttt tcttttgaag gtgtttttgc catgttagcc gcctttgtat
    10021 tggcagttcc gatggaccat tacctcactt ttttaccaat tgatgcgaat gttgcttttt
    10081 acagcttaat ggtttttttc attttggcag gtactgtttt aaatagcaga gcttattcat
    10141 ttgatgatgc ggcctttccg attgcaacta gtttttatgt tgggattggt tttcaacatt
    10201 tgatcaatgc tcgcctgtca ggaattgaca aggtcttttt ggccttattt atcgtttggg
    10261 ccactgatat tggagcatat ctgattgggc gtcagtttgg taggcgcaaa ctattgccta
    10321 cagtttctcc aaacaaaacc attgaaggta gtttaggagg gatcgcttgt gcagtccttg
    10381 tgtcttttat ctttatggtg attgataggt ctgtttatgc gccacatcac tttttgacca
    10441 tgttggtttt ggtcgctctt ttttccattt ttgcccaatt tggtgatttg gttgaaagtg
    10501 ctctcaaacg tcactttggt gtcaaagatt caggtaagct tatcccggga catggtggta
    10561 ttttagaccg ctttgattcc atgatttttg tttttcccat catgcatctc tttggtttat
    10621 tttaatctta agaggtaatg tttggagtcg caacaacgcg acaaaactca aaaaagcaat
    10681 agactgaaag gaaatctatg ttaggaataa taacctttat tattattttt ggtattttag
    10741 tgattgtcca tgaatttgga catttctatt ttgctaaaaa atcaggcatt ctagtaagag
    10801 aatttgccat tggaatgggc cctaaaattt tttctcatgt tgaccaagga ggaactcttt
    10861 ataccttgag gatgttaccc ttgggtggtt atgtgcgaat ggctggttgg ggtgatgaca
    10921 aaactgagat caaaacaggt actccagcaa gtttaaccct taatgagcaa ggttttgtta
    10981 agcgcatcaa cttgtctcaa agtaagttag acccaacgag tctcccaatg catgttacag
    11041 gctatgactt agaagatcag ctgagtatta ctggcttggt tttagatgaa accaagacat
    11101 ataaggtcgc tcacgatgct actattgttg aagaagacgg tactgagata agaattgctc
    11161 cgcttgatgt tcaatatcaa aatgctagta ttggcggacg tttaatcacc aattttgcag
    11221 gtcccatgaa taattttatt ttagggattg tggtttttat cctcttggtc tttttacaag
    11281 gtgggatgtc agattttagt agcaatcatg tccgtgttca agaaaatgga gcagcagcta
    11341 aggctggtct tcgagataat gaccaaattg tcgcaattaa tggttataag gtgactagct
    11401 ggaatgatct cactgaggct gtagaccttg caacacggga tctaggcccg tcacagacca
    11461 ttaaggtcac ctacaagtca catcagcgct taaaaacggt ggctgtgaaa ccacaaaagc
    11521 atgcaaagac atacacgata ggagttaagg cgagtctgaa aacaggattt aaggataagc
    11581 tcttaggcgg tctagaatta gcttggagtg gggcgtttac tattttgaat gctttgaaag
    11641 gattgatcac tggctttagt ctcaataaat taggtggacc tgttgccatg tatgacatgt
    11701 ccaatcaggc tgctcaaaac ggcttagagt cagtcttatc tcttatggca atgctttcga
    11761 tcaatttagg gatctttaac ctgattccga ttcctgcact tgatggggga aaaatcttga
    11821 tgaatatcat tgaagccatt cgtcgcaagc ctatcaagca agaaacagag gcctatatca
    11881 ccctagctgg ggttgctatc atggtcgtat tgatgattgc tgtgacatgg aatgatatca
    11941 tgcgcgtctt tttctaatca agataggtca tcttaagctg accttatcat gttaaagatc
    12001 gaaaaagacc tcaaccctaa gatttgcaag ggttgaggtc ttactcaaaa tgaaaggaat
    12061 acacattcaa attattgaat aatcccgctt cactcttact tgatgtttcc agaagggttt
    12121 gaggataagg ggatgtatta aaacggaata atctatgaaa caaagtaaac tgcttatccc
    12181 aaccttgcgc gaaatgccaa gtgatgccca ggttatcagc cacgcgctta tggtgcgtgc
    12241 cggttatgtg cgccaagttt ctgctggtat ctatgcttat ttaccactgg caaatcgtac
    12301 cattgagaaa ttcaagacca tcatgcgtga agagtttgaa aagatcggtg ctgttgaaat
    12361 gttggcacca gctcttttga cagctgatct ctggcgtgaa tcaggccgtt atgagaccta
    12421 tggagaggac ctctataagc ttaaaaaccg tgataactca gactttatct tgggtccgac
    12481 ccacgaagaa acctttacga ctttggtgcg tgatgcggtc aaatcttaca agcaattgcc
    12541 cttaaacctt tatcaaattc agtctaagta tcgtgatgaa aaacgtccgc gtaatggctt
    12601 gcttcgtacc cgtgagttta tcatgaaaga cggctatagt ttccatcaca actatgaaga
    12661 tttagatgtg acctacgaag attatcgtca agcttacgaa gctattttta ccagagctgg
    12721 tctagatttc aaagggatta ttggagatgg cggtgccatg ggggggaaag attcccaaga
    12781 atttatggcc gttacaccag ctcgtacgga ccttgatcgc tgggtggttc ttgacaagtc
    12841 tattgcgtca atggatgata taccaaaaga ggtcttagaa gatattaagg cagaattggc
    12901 tgcttggatg atttcaggtg aagatactat tgcttattca acagaatcaa gctacgctgc
    12961 caaccttgag atggcaacta acgaatacaa accgtcctca aaagtagctg ctgaagatgc
    13021 cttggcagaa gttgagacac cacattgcaa aacgattgat gaagtggctg cttttctttc
    13081 agtagatgaa acacaaacca tcaaaacctt gctttttgtg gcagataatg aacctgttgt
    13141 tgctttgcta gttggaaatg accatatcaa taccgttaaa ttaaaaaact atctagctgc
    13201 tgatttttta gagccagcta gtgaagaaga agcccgtgct ttctttggtg caggttttgg
    13261 ctcacttggg cctgtcaact tggcacaagg tagtcgcatt gtggctgacc gcaaagtgca
    13321 aaaccttacc aatgccgttg caggagctaa caaggatggt ttccatgtga caggagtcaa
    13381 tccaggacgt gacttccagg ctgaatatgt agatattcgt gaagtcaaag aaggggaaat
    13441 gtctccggat ggtcatggtg ttctccagtt tgcacgtggt atcgaagtcg gtcatatctt
    13501 caagttgggc actcgttatt cagacagcat gggagcaacg attcttgatg aaaatggcag
    13561 aacagttcca attgtgatgg gttgttatgg tatcggggtt agccgcattt tgtctgctgt
    13621 cattgaacag catgcccgtc tctttgtgaa caagacacca aaaggcgatt accgttatgc
    13681 ttggggtatt aacttcccta aagaattagc cccatttgac gtgcatttga ttacagttaa
    13741 tgtcaaagat caagtggccc aagacttgac ggcgaagtta gaagctgact tgatggctaa
    13801 agggtatgat gtcttgacag atgaccgtaa tgaacgcgtc ggttcaaaat tctctgatag
    13861 cgatttgatt ggtttgccaa ttcgtgtcac tgttggtaaa aaagccgctg aaggtatcgt
    13921 ggaaattaaa atcaaggcaa caggtgacag cattgaagtt aatgcagaaa acctcatcga
    13981 aacccttgaa attttaacaa aagaacacta aaaaagaaat gaagaggctg cctgtttaga
    14041 gtggcctctt tttgctgtga acttgtctga tacagagtgg cttttgcttt accaaaagcg
    14101 cttggcgaca aaagattggc aaaaaaatga cctcagctga gctatcactt ggaataaatt
    14161 agccaaagaa cgtcagaaga aagccctgct tttctttcca tctaaaactc gaaaatcatc
    14221 ttgaattgtg ataaaataaa gcctagtaaa atcaggagaa aattatgtca gatttattcg
    14281 ctaaattgat ggaccagata gaaatgccac ttgacatgag acgttcaagt gccttttcat
    14341 ctgctgatat tatcgaggta aaggtgcatt cggtgtcacg cttgtgggaa tttcattttg
    14401 cctttgcggc ggttttaccg attgcaactt atcgtgaatt acatgatcgt ttgataagaa
    14461 cttttgaggc ggctgacatt aaggtaacct ttgatatcca agctgctcag gtggattatt
    14521 cagatgatct gcttcaagct tattaccaag aagcttttga gcatgcaccg tgtaatagtg
    14581 ctagttttaa atcttctttc tcaaagctca aagtgactta tgaggatgac aaactcatta
    14641 ttgcagcgcc aggttttgtg aataacgatc attttagaaa cagtcatctg cctaatctgg
    14701 tcaagcaatt tgaagccttt ggctttggca ccttgaccat agatatggtg tcagatcagg
    14761 agatgactga gcatttgacc aaggattttg tttccagtcg tcaggctctt gtgaaaaagg
    14821 ctgtgcagga taatttggaa gcccaaaaat cccttgaagc catgatgcca ccagttgagg
    14881 aagccacacc tgctcctaag tttgactaca aggaacgagc agctaagcgt caggcagggt
    14941 ttgaaaaagc gaccatcaca ccaatgattg agattgagac cgaagaaaac cggattgtct
    15001 ttgagggtat ggtttttgat gtggagcgta aaacgactag aacaggtcgc catatcatca
    15061 actttaaaat gacagactat acctcctcgt ttgctctgca aaaatgggcc aaagacgatg
    15121 aggagctccg taaatttgac atgattgcta agggagcttg gttacgggta caagggaata
    15181 ttgagaccaa tccttttacg aagagtctca ccatgaatgt gcagcaggtc aaagaaattg
    15241 tccatcatga ccgcaaagac ctgatgccag aagggcaaaa gcgggtcgaa cttcatgccc
    15301 acaccaatat gtctaccatg gacgccttac cgacagtaga aagcttgatt gatacggcag
    15361 ccaagtgggg acacaaggcg gttgctatta ccgaccatgc taatgtgcaa agttttcctc
    15421 atggctacca tagggctcgc aaagctggaa ttaaggctat ttttggccta gaagccaata
    15481 ttgttgagga caaggtgcct atttcttatg atcctgttga tatggatttg cacgaagcta
    15541 cctatgtggt ctttgacgtg gaaaccacag gtctatctgc tatgaataat gacctgattc
    15601 agattgcggc ttccaaaatg tttaaaggca atattgtaga gcagtttgat gaattcattg
    15661 atcctgggca tcctctttct gcctttacca ccgaattgac agggattacc gataagcatt
    15721 tgcaggacgc caagccattg gttaccgtcc taaaagcttt tcaggacttt tgcaaagata
    15781 gtatcttggt tgcccacaac gccagttttg acgtgggctt tatgaacgcc aattatgaac
    15841 gccacgactt gcccaaaatc acacagcctg tgattgatac cttagaattt gctagaaact
    15901 tgtatcctga gtacaagcgt cacggtttgg gaccgctcac caagcgtttc caagtgagtc
    15961 tagaccacca tcatatggcc aattacgacg cggaagccac aggacgtctt ttgtttattt
    16021 ttctaagaga tgccagagaa aagcatggca tcaaaaatct tttgcaactc aatacagatt
    16081 tggtagctga ggattcttac aaaaaagcgc ggattaagca tgcgactatc tatgtgcaaa
    16141 atcaggttgg ccttaaaaat atgtttaagt tggtcagcct ttccaatatt aaatattttg
    16201 aaggggtgcc gcgtattcca agaaccgtct tagatgctca cagagagggt ttgttacttg
    16261 gtacagcttg ttctgacggc gaggtttttg atgccgttct gactaaagga attgatgcgg
    16321 cggttgattt ggctaagtat tatgatttta tcgaaatcat gccaccagcc atttaccagc
    16381 cattggttgt ccgtgaatta atcaaagacc aagcaggtat tgagcaggtg attcgtgacc
    16441 tcattgaagt agggaaacga gctaataaac ctgtacttgc cactggaaat gtgcattacc
    16501 tagagcctga agaagagatt taccgtgaaa ttattgtgcg tagtctcggt cagggtgcca
    16561 tgattaatag aacaatcggc cgtggggaag gggcccaacc tgctccccta cctaaagcgc
    16621 actttagaac aaccaatgaa atgctggatg agtttgcctt tcttggaaaa gacctcgctt
    16681 atcaagtggt tgtagaaaat actcaggatt ttgcggaccg tattgaggaa gtggaagtgg
    16741 ttaagggcga tctttacacc ccgtatattg acaaggccga agagacggtt gccgaattaa
    16801 cctatcaaaa agcctttgaa atttatggta atcctctccc agatattatt gatttacgca
    16861 ttgaaaaaga gttaacctct atcttgggga acggttttgc tgtgatttat ctcgcttccc
    16921 aaatgcttgt taaccggtca aatgagcgag gctatctggt tggctctagg ggatctgtag
    16981 ggtctagctt tgtcgccacc atgattggga ttactgaggt taatcctatg ccgcctcact
    17041 acgtttgccc gtcctgccaa cattctgaat ttatcacaga tgggtcagtt ggatctggct
    17101 atgatttgcc taataaaccc tgtccgaaat gtggcacccc ttatcaaaaa gatgggcaag
    17161 acattccctt tgagaccttt cttgggtttg atggggataa ggtgcccgat attgatttga
    17221 acttctctgg tgatgaccag cccagtgccc atttggatgt ccgagatatt tttggtgacg
    17281 aatacgcctt tcgtgctgga acagttggta ccgtagcaga aaaaacagct tatggatttg
    17341 tcaaaggcta tgaacgcgac tatggcaagt tctatcgtga tgctgaggtg gatcgtctag
    17401 cagcaggtgc tgctggtgtg aaacgaacga ctgggcagca ccctgggggg attgttgtta
    17461 ttcctaatta catggatgtt tatgatttta cccccgtgca atatccagca gatgatgtaa
    17521 cggcttcttg gcagacaact cactttaact tccatgatat tgatgaaaac gtcttgaaac
    17581 ttgatatcct agggcatgat gatccgacca tgattcgtaa acttcaggat ttatcgggca
    17641 ttgatcctat tactatccct gctgatgatc caggtgttat ggctctcttt tctgggacag
    17701 agattttggg cgttaccccg gaacaaattg ggacaccgac tggtatgcta ggcattccag
    17761 aatttggaac caactttgtt cgcggcatgg ttaatgagac gcatccgacc acttttgcgg
    17821 agcttttgca gttgtctgga ctatctcatg gaaccgatgt ttggcttggt aatgcacaag
    17881 atttgattaa agaaggcatt gcgaccctaa aaaccgttat cggttgtcgt gacgacatca
    17941 tggtttacct catgcacgca ggcttagaac cgaaaatggc ctttaccatt atggagcgtg
    18001 tgcgtaaggg cctctggctg aaaatttctg aggaagaacg taatggctat attgatgcca
    18061 tgcgagaaaa caatgtgccc gactggtaca ttgaatcgtg tggaaaaatc aagtacatgt
    18121 tccctaaagc ccatgcggcg gcctatgtct tgatggccct tcgggtggct tatttcaagg
    18181 tgcaccaccc cattatgtat tattgtgctt atttctctat tcgtgcgaag gcttttgaat
    18241 taaaaaccat gagtggtggc ttagatgctg ttaaagcaag aatggaagat attactataa
    18301 aacgtaaaaa taatgaagcc accaatgtgg aaaatgacct ctttacaacc ttggagattg
    18361 tcaacgaaat gttagaacgc ggctttaagt ttggcaaatt agacctttac aaaagtgatg
    18421 ctatagaatt ccaaatcaaa ggagataccc ttatccctcc atttatagcg ttagaaggtc
    18481 tgggtgaaaa cgtggccaag caaatcgtta aagctcgtca agaaggcgaa ttcctctcta
    18541 aaatggaatt gcgtaaacga ggcggggcat cgtcaacgct cgttgagaaa atggatgaga
    18601 tgggtatttt aggaaatatg ccagaagata atcaattaag tctttttgat gactttttct
    18661 agattatcat gatggtagtc atatgcaaag tcataaaaga agagggacaa gatcactttc
    18721 taatctctct gatactaaat tgtgacatac cgtataaagc gaggaaatca aatgagtcat
    18781 ttagataaga acaccgcttt gaaagccatg gtggtgtttc gtaaagctca gcgaacgcta
    18841 gatgcttttg gggctgatat ttttaaaaag gcagatttaa cagccacgca gttcagtgtg
    18901 cttgaagtac tatatactaa aggctgtatg cgaatcaatc acttgattga ttctttgtta
    18961 gcgacttcgg ggaatatgac tgttgtacta aataatatgg aacgtaatgg ctggattagc
    19021 aagtgcaaag acaaaacgga taaaagagcc tatgtggtta ccttaacaga taagggtact
    19081 cgcttgattg aagctgtttt gcctaaacac gtcgcacggg tggaagaagc ctttgctgtt
    19141 ttgactgaaa aagaacaact ttgtctgatt gaacttctca aaaagttcaa acaattataa
    19201 acaaatctct ttacagctct tcttagagaa gatataatta ctacatagtg ataaatttca
    19261 aaaagaagta tcaatgaaat accactttta gaaagcttta aaacgatctt taaaaaaaga
    19321 ggacaaagag atgaaacata ttttatttat tgttggctcg cttcgtgaag ggtcttttaa
    19381 ccatcaatta gcggctcaag cacaaaaagc tctggaacat caagcagtag tatcttactt
    19441 aaattggaaa gacgttcctg ttttgaatca agatatcgaa gctaatgtac ctttaccagt
    19501 tgttgacgct cgtcaagctg ttcagtcagc ggatgctatc tggattttta caccagttta
    19561 caacttctct attccaggtt ctgttaaaaa cctgctagac tggttgtctc gtgctcttga
    19621 tttgtctgat ccgacgggcc catctgctat tggcggtaag gtggttacgg tctcttcagt
    19681 tgcaaatggc gggcatgatc aagtatttga tcagtttaaa gcactattgc cgtttatccg
    19741 aacttcagta gcaggagagt ttacaaaagc aactgtgaat cctgatgcct ggggaacagg
    19801 aaggcttgag atttcaaaag agacaaaagc aaacttgcta tctcaggcag aggctctttt
    19861 agcggctatt tagactaaca aaaaaaggct aaagggcctt ttttgttagt ctaaaatcaa
    19921 gagctcttct ttggtttcaa aggggttttt agcattgatc cgatcgtaga acaaaacacc
    19981 attgatatgg tcaatttcat gttgaaccac aatagcgttg tatcccttta atttgatgcg
    20041 atgctgttgt ccttctttat catagtagtc tactgtaaca cgagcatggc gaaccacata
    20101 cccttcaaca acacgatcga cagataaaca accttcgcca tcagaaaggg ctgcatcttg
    20161 aacagaatga gaaacgattt taggattata caggacttct tgccagctat aggcttcttt
    20221 aggaggattt ccttctttat caggaagatt aggaacgagc acagcgatga tacgttttga
    20281 cacatcaatc tgaggagctg ctagaccaac accggcccta agtccaagtt tttccgccat
    20341 tacaggatct tgagaatgct tcaaaaattg catcatcttt tctcctaata ggatatcctc
    20401 atcacacaag ggtaagctca cttctttggc gacagctctt aaagttggat tcccttcacg
    20461 aataatgtca tccatggtaa tcagatgact aggtttaatt aatttatctt gtgcagacat
    20521 gtctactcct ttactttttt ttaactggat ttactatatc acaaatcatt tcttttttaa
    20581 agaaaagtca ggtagtggtc agagtgattg ctattataag ttaatgggct gcatacctca
    20641 gaggtctagt tattaacact taattgagtt agagcgtcta aatcggcaat acgacaggtt
    20701 ttgaagcaga tgcgttcgat aatcctctga aaaatgggct gcttaagtac gtgcttaaga
    20761 tggcgatcgc ttgttccaaa acggtttgct aataatgtaa ggtttagatg aacctcttgg
    20821 ttggcttgcg cttcaagggt ataggaagca aagcgctctt tgacggtata ggtaatgttt
    20881 tgttggcaaa cattttgtgt caactcttga cctatgtcat aaaggcaggt cgcatcggtt
    20941 aataagctgt gttcatggat tagggattgg gtttgttaat actagagcaa ttaatattat
    21001 cagtgaacac tttgttggaa agaaatgaat gactatgcta ggttaccgct tatcagctga
    21061 agtgcgggga tcagccatct tacctctttt agctggttat cttattaggc aagcaatcgc
    21121 acaggtttat tctgatttaa aaacagcctc ttggctattt ggccttgcta gttctgttgc
    21181 ttaaggtggt ttggatgtga gagcttagaa taataggata acggttgacc taatattatg
    21241 aatctgctag aatagagtgg tcgaaagaca aaccaacttt ttcttggttt taggagtgcc
    21301 aatcctaaga ctcggattaa gcaaaaaaag gagaatatat tatggcaatc tcaaaagaga
    21361 aaaaaaatga aatcattgct caatatgcac gtcacgaagg cgatactggt tcagttgaag
    21421 ttcaagtagc agttcttaca tgggaaatca accacttgaa cagccacatc aaagaacaca
    21481 aaaaagacca cgctacttac cgtggattga tgaaaaaaat tggtcaccgt cgtaacttgt
    21541 tggcatacct acgtcgtaca gatgttaacc gttaccgtga gttgatccaa tctcttggac
    21601 ttcgccgtta atttcaagtt taaaaagtcc tctgcttatg cagtgggctt tttcattttt
    21661 gacatgttgt ttgttaactt acacatttgg ctagaatggt ctaagactgt ttttttaagc
    21721 agttgatgat tttatttaac aacaaaaggt atctcagtga tgaggtatct tttgttgtat
    21781 catgaagaaa aagggaatca attcccagat ttgcctattt ttggccacct tttgaggaac
    21841 ttgttacaat gatatcacct gttgagtcca attgcagtca aaaatgacta cggacaattg
    21901 gactcaattt gtcttgaaaa tgaaaacgct tcattttata ctgacattat aggaggagat
    21961 gacatgctat ctcatgagct catcagaaac tatcaactct tttctaaata taaaggacat
    22021 tcactggagg catttgaatc catattaaag acaagcaaac gtcatatact ggcagatatt
    22081 gctaaaatca atgacacgtt gtcactctat cagttacccc ttattgctct ggacaggcag
    22141 ctggtttatc cgccagacct tactgaaaag gacttgttga atcgcatgct acctacctta
    22201 gacgactatc tctttcaaga tgagcgtctg gacatgatta tcatttacat catgatggct
    22261 aaagaattta tctccattaa ccacttggaa agcttgttac ggctcagcag aaattccgtt
    22321 attgctgatt taaacttggt gcgtgatcgg gtacaagctt ttcaggtaac tttagcttac
    22381 aatcgccagg atggttattt ttttgaagga gaacccttag ccttgcggcg tctcttagaa
    22441 tcagcggtca gttctctttt gcaagttaca tcaggacctt gggtgttcag ttacttattg
    22501 catgaacttg gtttgcccga ccagacaaag gtgatggcag caacgttgga ggaactgagc
    22561 agggagaacc acttaacttt tatttcggag aaattgaggg atttgattta ttttttctgc
    22621 cttctcgctc atcgaccatt ttcacggaac gtaagggctg aagtagtgga tacttttcct
    22681 ttagcttcgc cagctgttga aacgatggtg gaccagttat tggtcaactt ccccagtcta
    22741 accgaagaga aatatttggt tcaatctagg ttacttggtt gtatccaagg tgacttagag
    22801 ttagtctttc agcaacctat ttatgacatt atggaggaaa tcatcaattc ggtggcggtt
    22861 aacacgggat tgtctatcac cgatacccca gaacttcgtc agaacctata cagtcacctt
    22921 ttgccagctt attaccgact ttactacgac attaacttga ccaatccttt gaaggaacaa
    22981 atcaaacaag attatgaatc tctcttttac ttggtcaagc gcagcctttc tcctctagaa
    23041 aaacaattgg ggaaaccagt taatgaagat gagattgctt attttaccat tcattttggg
    23101 agatggttgc aggcccctaa gaagaggccg agcaatcagc tggtagcctt atctgtttgc
    23161 cctaatggca tcagttcgtc tctgatgttg gaggcaacct tgaaggaact ttttccacag
    23221 ctacagttta ttaggattca ccagctggac aaaataaagt tgttggatcc agcttccttt
    23281 gatttgattt tttcaacagt agcttttgac tgtgttaaac ctgtttatgt gacgcaagct
    23341 ttgatgggac ctgttgaaaa aatgatgttg aaaaagatgg tctgtgatga ctttcatctt
    23401 cccttgtcag agcaattcgc tttggatgac ctgttgagta ttattcataa acacaccacg
    23461 attactaata aagaaggact tgttagtgat ttatcccgtt acctgtttgg taaccattta
    23521 acgattgaaa aaggaggtct aggactattg gacttgttaa cagcagattt tattaggcag
    23581 gctgatgctg tttcggattg gcaggaagcg attcgtttgg ctgctcagcc tttactagaa
    23641 caccagatga ttgaaacatc ttatatcgac ggtatgattg attcggtcaa tgagcttgga
    23701 gcctatattg ttttagcccc taaggtggct gttcctcatg cggcgcctga aaaaggaacg
    23761 cggcagttag gcatgtctct cttacaacta aaagaacctg tcagctttga tttgaagcaa
    23821 gaaggtgatc cagacaagca agtgcaattg atttttgttt tgtctgctgt ggattctagt
    23881 tcacatttga aggctttgca ggaactgtcg ttgattttag atgatgatga acatattgag
    23941 caattaattg aggctaagaa tactgaggaa ataatgagtc tgattagcca tatgattgaa
    24001 aaaggagatg aatcacatga ttaaaattgt aacggtttgt ggaaacggta ttggtagtag
    24061 cttgttactt cgcatgaaag tagaagctat cgcctctagt ttgggtattg atgtggatgc
    24121 agaatcctgt gattccaatg cggctgttgg aaagggtgca gatttgtttg ttaccgttaa
    24181 agaatttaaa gatatttttc cagaggatgc caaggtttgt atcgttaaaa gctataccaa
    24241 tcgtaaaaaa attgaagaag atttggttcc ggttctcaag gaaatgagtg gcaaggaata
    24301 agcctttaaa ggagatagga agatactatg gaagcattat tatcatttat tcgagatatt
    24361 ttaaaagaac ctgcattttt aatgggcttg attgcctttg cagggttggt ggctttgaaa
    24421 acacctgctc ataaggtgtt gacaggaacc ttggggccga ttttgggata ccttatgctt
    24481 gctgcagggg cgggtgttat tgtgaccaat ctggaccctc ttgccaaact cattgagcac
    24541 ggttttagca tcactggcgt agtgccaaat aatgaagccg tcacttctgt cgctcaaaag
    24601 attctgggtg tggaaactat gtccatcttg gtggttgggt tattgcttaa tttggctttt
    24661 gctcgtttta cccgcttcaa atacattttc ttaacaggac atcacagctt ctttatggct
    24721 tgtctcttgt ccgccgtgct tggagctgtt gatttcaaag gaagtctttt gattatctta
    24781 ggtgggttcc tcttgggagc ttggtcagct atttcgccag ccattggtca acagtatact
    24841 ctgaaagtga ctgacggaga tgaaatcgcc atgggacact ttggtagttt gggctattac
    24901 ctttctgcct gggttggtag caaggttggc aaagacagta aagataccga ggaccttcag
    24961 atttctgaaa aatggagttt cttacgcaac accaccattt caacaggact tatcatggtg
    25021 cttttctact tggtggcaac agtggcttct gttttgagga atacttcagt aacagaagaa
    25081 ttagcagcag gtcaaaaccc atttatcttt gctattaaga gtggtcttac ctttgcggtt
    25141 ggtgtggcaa ttgtctacgc cggtgttcgc atgattttgg ctgacttgat tccagccttc
    25201 caaggtattg ctaacaaatt aattccaaat gctattccag ccgtggattg tgcggtattc
    25261 ttcccttatg cgccaaccgc tgttatcatc ggttttgcgt caagtttcgt tggtggctta
    25321 cttgggatgt tgattttagg agttgcaggt ggtgtcctga ttatcccagg tatggtgcct
    25381 catttcttct gcggtgcaac agcagggatt tttggaaatt caacaggtgg tcgtcgcggt
    25441 gcaatgattg gtgctttcgc taatggctta ttactcgcct tcttgccagc catgcttcta
    25501 cctgtacttg gtaaacttgg tttttcaaac acgacctttg gagatgtgga tttcggtgtt
    25561 ttagggattt tactgggccg cttaggaacg tcgattggtc aaatcggtat ttacttggtg
    25621 gttgctgtgc ttgctattgt attggtgctt ccaaactttc tgtcaaaatc tcaagaagcc
    25681 atcaataatg tttcagaaga aatctaaaag gaggatatga tgacaaaaaa actaaacgtt
    25741 aaagtctttt cagacggtgc ggtcttagag accatgttaa aggatttaca aacaggtttg
    25801 gtcacaggct ttacaaccaa tccaagtttg atgaaaaaag caggcatcag ttcttatatt
    25861 ggctttgcca aagaagtgct tgccaaaatt acagactacc cagtgtcctt tgaagtcttt
    25921 gcggatgatc tcgcaagcat ggaaaaggaa gctgagaaaa ttgccagtct aggtgacaat
    25981 gtctatgtga aaattccagt caccacttca acaggcgaat caacttgtcc cgttattcaa
    26041 aagttgtcag ccaaaggcat taagttaaac gtcacagcca tttttacaat tgagcagact
    26101 caagctgtgg ttgatcattt aaccgctgga gtgccagcta ttgtgtctgt ttttgctggt
    26161 cgtattgcag acaccggcgt tgacccaatg ccaatcatgg aagaagctct acgcatttgt
    26221 cgtcaaaaag aagaggtcga attgttgtgg gctagtcctc gtgaaactta taatatctat
    26281 caagctgatc agttaggcgt ggacattatc acttgtacca ctgatttgat tgccaagttg
    26341 cctcttcaag gaaaagattt agaagactat tcccttgaaa ccgtgcaaat gtttctgaaa
    26401 gacagtacca gcctcggctt taaaatttta gaggatgaca atcattaata ttaggagacg
    26461 aaagcaggca taccaacctg ctttttttgc tttgttaaag agtataagtt tcgtttaata
    26521 tttttcaagg acatttcaag agattgcgat ctgacagtga aatttctgct attttatggt
    26581 aaaatgtagc agatacgtcg tttgatacaa agaggagcgt gtgacaagcg tggcaggttg
    26641 ctttttgtag caaaacaacg tataacaact gaatctaagc agagaacagg tacctcttta
    26701 cccctctgaa aattaaaagg agaatatatg tcaaaacaaa cctttacaac aacatttgca
    26761 gggaaacccc ttgttgttga agttggtcaa gtcgctaagc aagccaacgg ggcaaccgtt
    26821 gttcgttacg gagaatcaac cgtcctgacc gcagctgtta tgtctaagaa aatggcaacg
    26881 ggtgatttct tcccccttca agttaattac gaagaaaaaa tgtatgccgc tggtaaattc
    26941 ccaggaggtt tcatgaagcg tgagggacgc ccttcaacag atgcgacctt gacagctcgc
    27001 ttgattgacc gcccgattcg tcccatgttt gccgagggtt ttcgtaacga agtacaagtg
    27061 attaacactg tcctttctta tgatgaaaat gctagcgctc caatggcagc gatgtttggc
    27121 tcatctctgg ccttgtccat ctcagatatt ccatttaatg gaccgattgc aggtgttcaa
    27181 gttggctata ttgatggtga gttcatcatt aacccagata aagaacagat ggaagcgtca
    27241 ctcttagaat taaccgttgc tggtagcaaa gaagccatca acatggttga atctggtgcc
    27301 aaagaattat cagaagacat catgcttgag gctcttttaa aaggtcacca agccattcaa
    27361 gaattaattg ccttccaaga acaaatcgta gcagttgttg gtaaagagaa agctgaggtt
    27421 gaactgttac aggttgatgc agacttacaa gctgatattg tggctaagta caatgctcaa
    27481 ctgcaaaaag ctgttcaagt tgaagagaaa aaagcgcgtg aagcagccac agaagccgtt
    27541 aaagaaatgg ttaaggctga gtacgaagag cgttacgcag aagacgaaaa cctagcaacc
    27601 attatgcgtg acgtggcaga aatccttgaa caaatggagc atgctgaagt gcgccgcctc
    27661 attacagagg acaagattcg tcctgacggt cgtaagattg acgaaattcg tccgcttgat
    27721 gctgtggttg acttcttgcc aaaggtccat ggatcaggtc tttttacgcg cggtcaaact
    27781 caggcgctat caattttgac cttggcacca atgggagaaa ctcaaatcat tgatggtctg
    27841 gccccagagt acaaaaaacg cttcttgcat cactacaatt tcccgcaata ttcagttggt
    27901 gaaacgggtc gttacggagc tgctggtcgt cgtgaaattg gacacggtgc cttaggtgaa
    27961 cgcgctcttg aacaagtctt gccaagctta gaagagtttc cttatgccat tcgtttagtt
    28021 gccgaagttc tagaatctaa cggttcgtct tctcaggctt ctatctgtgc tggtaccctt
    28081 gctctgatgg ctggtggtgt accaatcaag gctcctgtgg cagggattgc catgggtctc
    28141 atttcagacg ggacgaatta tacagtcttg accgatatcc aaggtcttga agaccacttt
    28201 ggagatatgg actttaaagt ggcaggaact cgtgaaggga ttacagccct tcaaatggat
    28261 attaagattg cagggattac gcctcaaatc ctagaagaag ctcttgcaca agctaaaaaa
    28321 gctcgctttg aaatccttga tgtgattgaa gcaactatcg ctgaaccacg tcccgagtta
    28381 gccccaactg caccgaaaat tgacaccatc aagattgacg tagacaaaat caaagtggtt
    28441 atcggtaaag gtggcgaaac cattgataag attattgccg aaacgggtgt caaaattgat
    28501 attgatgacg aaggaaatgt ctctatctac tcaagtgacc aagctgccat taaccgcact
    28561 aaagaaatca tcgctggcct agtacgtgaa gctaaggtag gcgaagttta ccatgctaag
    28621 gttgtccgta ttgagaaatt tggtgccttt gtcaacctct ttgacaagac agatgcactt
    28681 gttcacattt ctgaaattgc ttggactaga acgaccaacg tgtcagatgt ccttgaagtg
    28741 ggtgaagacg ttgatgttaa ggtcattaag attgatgaga agggtcgtgt ggacgcctca
    28801 atgaaagctt tgattccacg cccaccaaaa ccagagaaaa aagaagaaaa acatgactaa
    28861 atcaaatgaa ttagatattc gcttaagagc ttttatcaat gcgcctgata actttttgga
    28921 tagtgttgcc cttgtcaatg ctttccataa ttttcctgtc tgggctgcta aagaacccta
    28981 cgtgatagaa gtcgaaggtg tcaaggttac ccctgttttt accgataagg aagatatggc
    29041 tcgctttaaa gaagaacaag agagtgctca aagtcagtat tggctggagc gttcagctct
    29101 tgcagtgcta gaagaagtca ttacatctgg tgcagcaggt cttgtcttta accttaagaa
    29161 aaagggagat tttgggaatt ctaccatttt caaaagcagt gatatgattc aatttatgaa
    29221 ccattacacc actgtgttaa atacgcttat gagtgatgac aatgtggcgg cagatacgat
    29281 ggaaaaggtt taccttgttc ctgcctttgt ttatcctaaa gataataacc attacgaccg
    29341 cctttttcct accatgtcaa cgcctgaagg aaaaagctat gttcccgcct tttcaaatct
    29401 tcaaagcttt gcgaaatggt acaaccaaga cgattttgga ggccttttca ggaaagctga
    29461 aggagtgatt ctgacttgga cgattgatga tatttatcaa ccaagaaatg gtgaaaacga
    29521 gcttgatgaa acgttcggtg tcgccatcaa tccttttgat gaccaacaaa ttcttgttga
    29581 ttggtcagaa ttagataagt cgtaggagtt agtatgggtt ggtggaaaga aagtatagct
    29641 attgttaaag ctctagaccc agctgctcgt aatagtcttg aggttatcct aacttatcca
    29701 ggcatcaaag ccctagctgc tcatcgtttg tctcattttc tttggcgaca tcattttaaa
    29761 ttactagcca gaatgcatag ccaattttgg cgattttgga cacaaattga aatccatcct
    29821 ggagcacaaa tcgctcctgg agtcttcatt gaccatggtg ctggtcttgt tattggagag
    29881 acagcaattg ttgaaaaagg tgtgatgctt tatcatgggg tgaccctagg tggaaccgga
    29941 aaagattgtg gcaagcgtca tccaacggtt cgacaaggtg ccttaatttc ggcacatgcc
    30001 caagtgattg gacctattga catcggagca aatgctaaag taggggcagc agctgttgtg
    30061 ttatcagatg ttcctgaaga cgtgacagtt gtaggtgtgc cagctaagat agtacgagtg
    30121 catgggcaaa aagataatcg tcaaattcaa agtttacaaa aacaacgaga ggtctcttat
    30181 cagttgtcaa aatgatcgga aaataacaaa aagaagcact gatgacttcc gataaaatat
    30241 tttgggactt tctagtagag taatgttgaa cttagctagc cctatcagtt tggttgctga
    30301 gtgtagtgat atgggaatgt tacaaaatac aaaaaacacg taaaagagca tcaactaagc
    30361 aagtcttaga ctgaagaaac agtctattgt gatacttttt ctcaggtggt gcttacggca
    30421 gcgacttcct ttggaattcc atgccttagt tttgagccta aggtctcaaa aatctgtatg
    30481 ggcatcactg tcagtattga taccactttt accacaacaa aaagcagctg ttttggctca
    30541 ccccacatgc agaaatagga aacctgatgt caaatcagca gatctattca tgctttttga
    30601 gcataaaagt gggtttgtct actttctgaa gccttcacag gttgtgaagg tttttcgtta
    30661 ataaaggagc ctcatgaata aaataagagt tgggctgtat gtcattatga gtttgtctat
    30721 tcttgtttct agtattacac tggtggctag tctaatcttt ttactgagac aagacatggt
    30781 taaagcgatt atatacttca ttttgaccct attttggtta ataggtttgg ttattaatat
    30841 taaattgttg aaaaggtgga atagcatttc agaaaaagac ttaggatatc aagatgacaa
    30901 ctaatgctag gatattgaat aaggagttta catgattaaa atttatgata ccatgacccg
    30961 ttcgctccgc aagtttgtac ctttgactga aaatacagtc aatatgtacg tttgtggacc
    31021 gacggtctat aattatattc atattggaaa tgctagatca gcggtcgctt ttgacaccat
    31081 tcgacgttat tttgagtata ctggctatca ggtcaattac atttccaatt ttaccgatgt
    31141 cgatgataaa attatcaagg ctgctactca agcaggtgtt tctcctaaag aattgtcaga
    31201 tcgctttatt gcagctttta tagaagatac caaggcactt ggtgttaagc cagccacaca
    31261 aaatcctcgt gttatggatt atatagcaga aatcatttca tttgttgaaa gcctcattga
    31321 aaaagatttt gcttacgaag cagatggaga tgtgtatttc cgcgtggaaa agtcagagca
    31381 ttatgccaag ctagctaata aaaccctgtc agaacttgaa gttggagcca gtggtcgaac
    31441 agatgctgag acagctttaa aagaaaatcc actggacttt gctctttgga aatcagctaa
    31501 ggcaggtgag gtttcttggg atagcccctg ggggtttggt cgtccaggct ggcacattga
    31561 atgttctgtg atggctactg aaattcttgg tgataccatt gatattcatg gtggcggagc
    31621 tgatttggaa tttcctcatc ataccaatga aattgcccaa tctgaggcta aaacaggcaa
    31681 gacttttgct aactattgga tgcacaatgg gtttgtcact gttgataacg aaaaaatgtc
    31741 caagtcacta ggtaattttg tgaccgttca tgacatgtta caaaccgttg atggtcaggt
    31801 tttgcgattc tttcttgcga cacaacagta ccgaaaacca attaacttca ctgaaaaggc
    31861 tattcatgat gcggaaatca atctgaagta tcttaaaaac accttgcaac aaccgctgac
    31921 agaaactgca gatgagcaag agctaaaaca gtttgtaata gctttccaag acgctatgga
    31981 cgatgatttc aatacagcaa atgggattac cgtcgtattt gacatggcta agtggattaa
    32041 ttctggctcc tatacagaac ctgttaaaag cgcttttgag aaaatgttag ctgtttttgg
    32101 cattatcttt gaagaagagg tgcttgaggt tgacattgaa gccttgattg ctaagcgaca
    32161 agaagcacgc gcgaatcgtg attttgccat agctgatgcc attcgagatc agttagcagc
    32221 tcaaggcatc aagctgctag ataccaaaga tggtgtgagg tggctgcgtg attaatccag
    32281 ttgatgtgaa tttgattaat ggcattgccc tagcctttga aggagatgcg gtttattcct
    32341 actatgttcg tcgtcatctc atttttcaag gtaaaacgaa acctagccag ctacaccgtt
    32401 tagcaacgag atatgtttct gctaaggcac aagccaactt gattcaggct atgttagaag
    32461 cgcagctatt gaccgaaaaa gaagaagaca tctataagcg tggtcgcaat accaatagcc
    32521 atactaaagc taagaatgcc gatattatta cctatcgtat gtcaacaggt tttgaagcca
    32581 ttatgggtta tcttgatatg atgggccaaa aagagcggtt agaagaattg atcagatggt
    32641 gtattgagta tgtagaaaag caacaattga tatcctcata agagtcccct aggagactct
    32701 tattttcata ggtctgaagt aaaggtagag agccattaac tctttttatg acatcaaaaa
    32761 gacataatga tataaaaaag atacatttgt aacaaaaaag acataaaaat aatgaaatca
    32821 ttttcgaata gtttataatg agagggtaag actaatttaa agaggagata tttattatga
    32881 caactatgca aaaaacagtt agcttattat cactagcttt acttattggt ttgctgggga
    32941 cttctggcaa agccatatct gtgtatgcac aagatcagca cactgataat gttatagctg
    33001 aatcaactat tagtcaggtc agtgttgaag ccagtatacg tggaacagaa ccttatattg
    33061 atgctacagt caccacagat caacctgtca gacaaccaac tcaggcaacg ataacactta
    33121 aagacgctag tgataatact attaatagtt gggtatatac tatggcagcg caacagcgtc
    33181 gttttacagc ttggtttgat ttaactggac aaaagagtgg tgactatcat gtaactgtca
    33241 ccgttcatac tcaagaaaag gcagtaactg gtcaatcagg aactgttcat tttgatcaaa
    33301 acaaagctag aaaaacacca actaatatgc aacaaaagga tacttctaaa gcaacgacga
    33361 attcggtcga tgtagacaca aaagctcaaa caaatcaatc agctaaccaa gaaatagatt
    33421 ctacttcaaa tcctttcaaa tcagctacta atcatcgatc aacttcctta aagcgatcta
    33481 ctaaaaatga gaaacttaca ccaactgcta gtaatagcca aaaaaacggt agcaacaaga
    33541 caaaaatgct agtggacaaa gaggaagtaa aacctacttc aaaaagagga ttcccttggg
    33601 tcttatcagg tctagtagtc agtttagctg caggtttatt tatagctatt caaaaagtat
    33661 ctagacgaaa ataaaagagt ctatgcattt attttgaagc attcatggta taataaactc
    33721 atggaagata aagatactat tgaaacaaac gatatcgtct atggtgttca tgccgttaca
    33781 gaaagccttc aagcaaatac aggaaataag ctttatatcc aagaggattt aagaggaaag
    33841 aaagtggata acatcaaaag cttagcgaca caaaaaaagg tcgctatttc atggacgcct
    33901 aaaaaaacct tgtcacaaat gactgatgga gctgtgcatc aaggttttgt tctgagagta
    33961 tcagcttttg cctatactga tgttgatgag atcctcgaaa tagcagagca agaagcaaat
    34021 cctttgattc ttattttaga tggtctgaca gatcctcata atttaggatc gattttacgg
    34081 acagctgatg ctacaaatgt atgtggagtg attattccta aacatcgttc tgttggtgtg
    34141 actccagtgg tctcaaagac gtctacaggt gctgttgaac atattccgat agcgagagta
    34201 actaacctta gtcaaactct agataaattg aaagcaagag gattctggat ttttggcaca
    34261 gacatgaatg gaacaccgtc tgattgctgg aacactaatg gtaaacttgc tttagtcatt
    34321 ggtaacgaag gcaaaggcat ctctaccaat atcaaaaagc aagttgatga aatgattacg
    34381 attcctatga atggtcacgt acagagctta aatgctagcg tagcggcagc tattctcatg
    34441 tatgaagttt tccgaaatag gcgctaaatt atgaaaaaac ggatattatt agtagatggg
    34501 tataatatga ttgccttttg gcaatcaacc cgtcagttgt ttaagataaa tcagcttgat
    34561 caggcacgta acacactttt aacaaaactt aatcattatg cccattttga gaatattaat
    34621 attatttgtg tttttgatgc ccaatatgta ccggggttaa ggcaacgata tgaccagtat
    34681 tatatctcag tggtatttac agaggaagac gaaacagcag atagctacat tgagcgcatg
    34741 gcagcagagt taaatacggc tatacatatg gtagaagtag ctacgagtga tttaaatgaa
    34801 caatggacga ttttttctca aggagctctt cgtgtcactg caagagaatt agagcaaaga
    34861 gttcatactg tcaaagctga tttagataaa atgtctagag atattgatct caaaacgcct
    34921 aaactgcggc cttttgacca aggacagctt attcaattga aggattttat gtctcagtta
    34981 gatcgataat gagaattacg actcgccaag tgcgggtttt tttgataaaa taaaacaata
    35041 attatttgat tatcaaacta aaaaactgga tgaggagaag taagaatgac ctttacaata
    35101 atgacagatt caaccgctga tttgaatcaa acctgggcag aagatcatga tattgtcctt
    35161 ataggattaa cgattttgtg cgatggagag gtttatgaaa cggtcggtcc taaccgtatt
    35221 agtagtgatt accttttgaa aaagatgaaa gctggcagtc atcctcaaac cagtcagatt
    35281 aatgtgggag agtttgagaa ggtttttcgt gaacatgcta ggaacaacaa agcactgctt
    35341 tatcttgctt tttcttcggt tttatcgggt acctatcaaa gcgctctaat ggcacgcgat
    35401 cttgttcgag aagattatcc cgatgcagtg attgaaattg ttgatacctt ggctgctgca
    35461 ggaggagaag gctatctaac cattttagca gcagaagcca gagatagtgg caaaaattta
    35521 ctggagacta aagatattgt tgaagcagtg attcctcgac tgcgcaccta tttcttagta
    35581 gatgatcttt ttcatttgat gcgtggtggg cgtctgtcaa aaggttcagc ttttctaggc
    35641 agtttagcca gtatcaaacc tcttttgtgg attgatgagg aagggaaatt ggtgcccatt
    35701 gcaaaaattc ggggacgcca aaaagccatc aaagaaatgg tggctcaagt ggaaaaagat
    35761 attgcggatt cgacggttat tgtctcttat acaagcgatc agggtagtgc cgaaaagtta
    35821 cgagaagagc tgctggcgca tgagaatatt agcgatgttc ttatgatgcc actaggacca
    35881 gttatctcag ctcacgttgg tcctaacacc ctggcagttt ttgtgattgg gcagaattct
    35941 cgttaatccc ttataaggat tgaagagaaa tcctcaatcc ttttttgctg cttaaatcaa
    36001 tcaagaatgt aaaatatatt tgacaaaata tccaaaataa tttatattat gtcatataaa
    36061 gatttaaagg agaaacgaat catgtttgat agcaaacaaa atctcgctaa gtcacaatgg
    36121 ggtttcattg tcataacagc agtgatggat ctagttgtga tagtagcaac attactggct
    36181 aagaccagtt ttcaacgtta cttagttgga ggcgtagcgg ttatattgac tagctttctc
    36241 atattgttgg tatgggggct aaagacagcg aaaaagctcc aaacgagact agatagcaaa
    36301 gaagtactag attctaaggt aagggacgat cagaagctgc ccagatatga cgaacgccaa
    36361 aaacagattc ttctcaaagg atacactatt gggttttggt ttatgattgt ggctgtttgg
    36421 ctgtcaatct ttcttcatcg cttctcagag agactggtat ctgcaagctt cctctttaca
    36481 ttggctcttt ggggagggtt ggcagttcaa acgacatatt gtaacctcaa cggggtatcg
    36541 ccatttgtgg ataggcgctt tggaaaacaa ggcatactgg tggggatgat agcaacagga
    36601 atgagtttaa tcactatagg tttggcggta agagagatga tggcagggca acttaaaatg
    36661 ggaagctttt tcaaggctgg cggcagtgga tctttaatgg tgttgggcat aacgctcctt
    36721 agtatgggag ctagtatttt ctatcgtcat tatctggatg ctaaagaggc agatgaatga
    36781 agaacctcaa attaaaagca gcgcgtgcag gcaaagattt gtctcaacaa gctctagcag
    36841 atttggtagg cgtctccaga caaaccattg cggctgttga aaaaggtgat tacaacccaa
    36901 cgattaatct ttgcattgcc atttgtcgag tacttgataa gaccttagat gacctttttt
    36961 gggaggttga tgattcccat taacatcagc agagcagctc tgccattcct caaaaacgaa
    37021 agaaaaaata atccaataaa atcagctaaa gcacttgact ggagcctctt aagtttgata
    37081 tcatagtagg cggtattgtt taccccattt gaaaggcccc ggaaccttcc aaataccttc
    37141 gatgggatgg aacacccatc accttgtaaa caaatattac gaattcgtat aggagaaatc
    37201 atgaacaaaa caactttcat ggctaaacca ggccaagttg aacgcaaatg gtacgttgtt
    37261 gacgcaactg atgtgccact tggacgtctt tctgcagtag ttgctagcgt acttcgcgga
    37321 aaaaacaaac caactttcac accacatact gatacaggtg attttgttat cgttatcaac
    37381 gctgaaaaag ttaaattaac tggtaaaaaa gcgactgata aagtttatta cactcactca
    37441 atgtacccag gtggtttgaa atcaatcact gctggtgaac ttcgctctaa aaacgcagtt
    37501 cgcttgattg aaaaatcagt taaaggcatg cttccacata acactcttgg acgtgcacaa
    37561 ggtatgaaat tgaaagtctt cgttggcggt gagcacactc acgcagcaca acaaccagaa
    37621 gtacttgaca tctcaggact tatctaagag gaaaggagca ttaaataatg gcacaagcac
    37681 aatatgcagg tactggccgt cgtaaaaacg ctgttgcacg cgttcgtttg gttccaggta
    37741 ctggtaaaat cactgttaac aaaaaagatg tagaagaata catcccacat gctgacctac
    37801 gtttgatcat taaccaacct tttgcagtta catcaactga aggttcatac gacgttttcg
    37861 ttaacgttgt tggtggtggt tacggtggac aatcaggtgc gatccgtcat ggtatcgctc
    37921 gtgcgcttct tcaagtagac ccagacttcc gcgattcgtt gaaacgcgct ggccttctta
    37981 cacgtgacgc acgtatggtt gaacgtaaaa aaccaggtct taagaaagct cgtaaagctt
    38041 cacaattctc aaaacgttaa gaacagcttt acatcaacga ttacaagcat tttgcaagtc
    38101 tcttgcaagg tgcttttttt gtcgtttttt gcttttgata cctttttttg ataccgtttc
    38161 ggaaaacttt tgacttggtt taatacgttt atttagttat ttttgagata gttagcaaat
    38221 tgtttgattg atttgtcttc tccgactgca gtagcatgac tgtatgtatc aagagttatt
    38281 tgactactag catgtcctaa ccttttggct gtatttactg ggtcaactcc aatctcaatc
    38341 atcagggttg catgggtatg cctgaagccg tgaggactta ttttttaaga ctatgttgct
    38401 taatgacctt tgataagata ttgttaatat agtctgtatg aagtggttca atactccctt
    38461 ctctagtaca gtatgtaaat ataaagtcag actcaattga aagaggtgca actgctagtt
    38521 gtgccttttc tttttggctg atggctttcc attcttgcag aatagaaata gtttcgctgt
    38581 ctagcttaat ctttcttact gagaaatcat ttttagtgct tttttcgatt gcctgtccgt
    38641 ctaatcgtcc taggctctta ttgacgctta gtgtctcact ctgaaagtct atattagccc
    38701 atttaagagc gcatagctcg cctttcctta aggccgctat aagctagtag ccgaaatagt
    38761 gcgtagtgct tgtaaggctc tttctgaagt actatggcta aaaactcctg aagttcttga
    38821 accgtccaat agttttctat tcgtgtcttt ttccgctttg gcataatgat ttcagccata
    38881 gggttatgct tgagcagttt catcttgatg gcaaaatcaa agatcttttc agtgtaggac
    38941 ttgatttgct tgatattctt aaaggtcttt gctttatcgg tgatgaagtt ctggcagtct
    39001 aaaggggagg ttttgttaat gggcaattct cccatgactg gcaaaatgtg aagttgaaac
    39061 agatcaagag tacgagaagc cgtggttggc tctaccgtat cttgataggc ttgataccat
    39121 tcctcgaata tctcgctata agttttatga gttggcttgg tgaactcctc taagtttcct
    39181 gaagccttaa ggtagattag cctatcttcg tagtctttgg cttctttgtg gaatatctac
    39241 taaacagccc gaacttattt tgctcctagt taattggtaa tctaccaaaa aagccctagc
    39301 aagactgcta aggcttttaa tgtgttattt gaataacttt atatagtcgc tttgagtagg
    39361 aagcaagtaa tcaataacga ccctattttc ttccccctcg atatggtata ggacaatata
    39421 atctttactt aaggtataac ctttagtgct gtgataatgg atgatttttg aaccatattt
    39481 ttcatcggca tcaaaaccaa cttctcggaa aacctcgagt ttttctatgt cgctaatgat
    39541 ttgctccatt ttacgctgac ctgatgttga ggaatagttt tgagaaatat aatcacgaat
    39601 ttcttttagc ttctctaaaa catcaggagc atagataatc tgatatttct tatagtccaa
    39661 gttcagccct cacttcttca gaggtataaa acttcccttg acgaacatcc tcaatatttt
    39721 tgttgatttc agcttgaaat tgcttgaaaa gtttttcttt ctctaactcc tcttccgtca
    39781 ataagtcaac ctcgtttgtg acaacgacat tttgtaggaa taatcttaaa gcagatgata
    39841 aggttaaatt ttttttgttt aatactgtca ttgcttcgct taccaattct ttattagctt
    39901 gaaaggtaac cgctctgttt tttttactgt agtcatttta tgtaacccct ttatgtaatt
    39961 tatttaatta tatcatatat acaaaaaagt caataaaaaa gactcggtaa ttgagacaga
    40021 ttttcattat ttgataagct ccgaaactta tttt
//
LOCUS       DATUYR010000012        39779 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.012, whole genome shotgun sequence.
ACCESSION   DATUYR010000012 DATUYR010000000
VERSION     DATUYR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39779
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.012"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..162
                     /locus_tag="VCI33_000545"
     CDS             <1..162
                     /locus_tag="VCI33_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEP2831487.1"
                     /translation="TVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYAD
                     HFDKLVNKD"
     gene            complement(415..3954)
                     /gene="smc"
                     /locus_tag="VCI33_000546"
     CDS             complement(415..3954)
                     /gene="smc"
                     /locus_tag="VCI33_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEP2831488.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFYKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLKQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGTNRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKSALTCDIDDIKENKDLVR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAINQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIRKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(3955..4647)
                     /locus_tag="VCI33_000547"
     CDS             complement(3955..4647)
                     /locus_tag="VCI33_000547"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEP2831489.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVRD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            complement(5080..5889)
                     /locus_tag="VCI33_000548"
     CDS             complement(5080..5889)
                     /locus_tag="VCI33_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP2831490.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            complement(5893..7245)
                     /gene="vicK"
                     /locus_tag="VCI33_000549"
     CDS             complement(5893..7245)
                     /gene="vicK"
                     /locus_tag="VCI33_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEP2831491.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPIISVWIEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            complement(7238..7948)
                     /locus_tag="VCI33_000550"
     CDS             complement(7238..7948)
                     /locus_tag="VCI33_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2831492.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            complement(8110..9144)
                     /locus_tag="VCI33_000551"
     CDS             complement(8110..9144)
                     /locus_tag="VCI33_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEP2831493.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            complement(9200..10447)
                     /locus_tag="VCI33_000552"
     CDS             complement(9200..10447)
                     /locus_tag="VCI33_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEP2831494.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDSELLKKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            complement(10404..11552)
                     /locus_tag="VCI33_000553"
     CDS             complement(10404..11552)
                     /locus_tag="VCI33_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEP2831495.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            complement(11947..12096)
                     /locus_tag="VCI33_000554"
     CDS             complement(11947..12096)
                     /locus_tag="VCI33_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEP2831496.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            complement(12182..12967)
                     /locus_tag="VCI33_000555"
     CDS             complement(12182..12967)
                     /locus_tag="VCI33_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831497.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFVIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            complement(12969..13787)
                     /locus_tag="VCI33_000556"
     CDS             complement(12969..13787)
                     /locus_tag="VCI33_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEP2831498.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLMVLSGIKVMAGLSTLQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKVVYIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            complement(13789..14781)
                     /locus_tag="VCI33_000557"
     CDS             complement(13789..14781)
                     /locus_tag="VCI33_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP2831499.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEKTTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELKPGQEQVVSQFMGLPDIT
                     VERHELSLDIQYDSSRYQTADIIQKTMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            complement(14922..16865)
                     /gene="thrS"
                     /locus_tag="VCI33_000558"
     CDS             complement(14922..16865)
                     /gene="thrS"
                     /locus_tag="VCI33_000558"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEP2831500.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTRKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSCPDAQA"
     gene            complement(17287..18621)
                     /locus_tag="VCI33_000559"
     CDS             complement(17287..18621)
                     /locus_tag="VCI33_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP2831501.1"
                     /translation="MRVGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(18623..19621)
                     /locus_tag="VCI33_000560"
     CDS             complement(18623..19621)
                     /locus_tag="VCI33_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP2831502.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADSKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(19752..20753)
                     /gene="ccpA"
                     /locus_tag="VCI33_000561"
     CDS             complement(19752..20753)
                     /gene="ccpA"
                     /locus_tag="VCI33_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEP2831503.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            20927..22012
                     /locus_tag="VCI33_000562"
     CDS             20927..22012
                     /locus_tag="VCI33_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP2831504.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYVEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLVKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            22061..22726
                     /locus_tag="VCI33_000563"
     CDS             22061..22726
                     /locus_tag="VCI33_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP2831505.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            22737..23114
                     /locus_tag="VCI33_000564"
     CDS             22737..23114
                     /locus_tag="VCI33_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP2831506.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            23259..24185
                     /locus_tag="VCI33_000565"
     CDS             23259..24185
                     /locus_tag="VCI33_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP2831507.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            24396..25079
                     /locus_tag="VCI33_000566"
     CDS             24396..25079
                     /locus_tag="VCI33_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEP2831508.1"
                     /translation="MEWIKLIGIVIIVLGFILKFDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            25079..26005
                     /locus_tag="VCI33_000567"
     CDS             25079..26005
                     /locus_tag="VCI33_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEP2831509.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            26055..26702
                     /gene="pcp"
                     /locus_tag="VCI33_000568"
     CDS             26055..26702
                     /gene="pcp"
                     /locus_tag="VCI33_000568"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEP2831510.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(26819..27538)
                     /locus_tag="VCI33_000569"
     CDS             complement(26819..27538)
                     /locus_tag="VCI33_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEP2831511.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(27544..28011)
                     /gene="smpB"
                     /locus_tag="VCI33_000570"
     CDS             complement(27544..28011)
                     /gene="smpB"
                     /locus_tag="VCI33_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEP2831512.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            complement(28014..30347)
                     /gene="rnr"
                     /locus_tag="VCI33_000571"
     CDS             complement(28014..30347)
                     /gene="rnr"
                     /locus_tag="VCI33_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEP2831513.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINSQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYSQPSQEKRDHFTQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGTK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(30441..30677)
                     /gene="secG"
                     /locus_tag="VCI33_000572"
     CDS             complement(30441..30677)
                     /gene="secG"
                     /locus_tag="VCI33_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEP2831514.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(30723..30869)
                     /gene="rpmG"
                     /locus_tag="VCI33_000573"
     CDS             complement(30723..30869)
                     /gene="rpmG"
                     /locus_tag="VCI33_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP2831515.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(30866..32059)
                     /locus_tag="VCI33_000574"
     CDS             complement(30866..32059)
                     /locus_tag="VCI33_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEP2831516.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPKELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVAPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(32181..33683)
                     /locus_tag="VCI33_000575"
     CDS             complement(32181..33683)
                     /locus_tag="VCI33_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEP2831517.1"
                     /translation="MTTMTIAKGSQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVIQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSEI
                     ESQQLVQSSPFASKYSQSIDRESAYEKLAAKVLEDNRLAQEAIATAKREKEAKEAIKA
                     QAATKKASRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(33873..34466)
                     /locus_tag="VCI33_000576"
     CDS             complement(33873..34466)
                     /locus_tag="VCI33_000576"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEP2831518.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPILMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNSGDIAALIKQVQSALLSLANPR"
     gene            complement(34463..35290)
                     /gene="mutM"
                     /locus_tag="VCI33_000577"
     CDS             complement(34463..35290)
                     /gene="mutM"
                     /locus_tag="VCI33_000577"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEP2831519.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPGTASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(35462..36328)
                     /gene="rgg2"
                     /locus_tag="VCI33_000578"
     CDS             complement(35462..36328)
                     /gene="rgg2"
                     /locus_tag="VCI33_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEP2831520.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            36417..36485
                     /gene="shp2"
                     /locus_tag="VCI33_000579"
     CDS             36417..36485
                     /gene="shp2"
                     /locus_tag="VCI33_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEP2831521.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            37399..37668
                     /gene="stcA"
                     /locus_tag="VCI33_000580"
     CDS             37399..37668
                     /gene="stcA"
                     /locus_tag="VCI33_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEP2831522.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQTPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQY"
     gene            complement(38016..38414)
                     /locus_tag="VCI33_000581"
     CDS             complement(38016..38414)
                     /locus_tag="VCI33_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEP2831523.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(38511..39581)
                     /locus_tag="VCI33_000582"
     CDS             complement(38511..39581)
                     /locus_tag="VCI33_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831524.1"
                     /translation="MMMLRDVKVKMSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGSSGAFPDNIK
                     VSINPRGGQATIIYGDGSTDIIPSAVLWEKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
BASE COUNT        11909 a         8522 c         7015 g        12333 t
ORIGIN      
        1 acagtttttc tctatgcaac aggttacttt gaatttaagc gccaattaga aactggagtt
       61 gaaaagatga aacaagccat acaagttctt gatattttaa atgaagacaa gttaaaatta
      121 cattacgctg accattttga taaactagtc aacaaagatt aagtgaaatc attctttacc
      181 ttttaacgaa atgccaatca agagtgtctc aataaaaaat catctactct gacaactttt
      241 ctcttttacc tatcgacagc taaaaagcct tggaaactgc tatttccaag gctttctttg
      301 tactctattg tatcaattag cttattccga tttctgttgc tattagttta atcatgaaat
      361 ctcctaactt atgaccgaac atccaaaaaa acagaaggaa ctcccttctg ttttttaatt
      421 ggtcatctct tgagcttctt ttaattttac agatacaatt ttagagaccc ccgattcctg
      481 catggtaatt ccataaatac tatctgctgc agccattgtt cctttacgat gggtcacgac
      541 aataaattga ctatctttgt caaagcgatt gaggaaatcc ccaaagcgct taacatttgc
      601 ttcatcaaga gccgcttcta cctcatctaa aatcacaaac ggaatcgttt taacacgtat
      661 aatagcaaac aataaagcca gtgctgacaa agctttctct ccgcctgaca tcaagttaag
      721 agattggatt ttcttaccag gaggctgcac agaaatctct ataccagcag acaagagatc
      781 tgtttccgtt aaaaccaaat cagcagaacc cccaccaaac atctgagtaa aggtttcctt
      841 aaaacttttt cgaattgcct caaaagtcac tttgaaacga gccttcactt cgctatccat
      901 actattaata gtctctagca aaagattttt agcttttgtc agatctgtct tttgacttgt
      961 taaaaaagtc aaacgctcat gcacttcctc atactgatta atagcatcac tattaattgg
     1021 tcctagtgca cgaatagtct tttgtaaatg gtgtaatttt tctttggcac tttctaagtg
     1081 atcaatacta tttgttacct cctttgcgtc tgctagtgtc atttgaaaat cttctgataa
     1141 acttttagca taggctcgta agcgattagc cacttgctct aaatgtgtct ctaacttggt
     1201 ttgacgacga ataaatgtgt catttttttc gctttctttg gttatttttt ctgctgtttc
     1261 ttctaagcgg gcctcatagt cctctatttc aaatcttaat tggactaaac gtttttgagc
     1321 accagactta tgctctgtgg catcttgtaa ctgtttttgc cactggggta gtctttgaat
     1381 gctgtcttga cttacgttgt tgtttagtat gctttcaagt ttgagaatat tttgctggca
     1441 ttgcttcagc tgagttctta gacgagattg attagcttgc tcaaatttct tttcattaag
     1501 taaatctctt tcttgcaaac gagcttgaga taaagcttgg tgaatgtttt gtgttttttg
     1561 cctaaccaaa tctttgtttt ctttgatatc atcaatatca caagttaagg cacttttttt
     1621 cttagcaata gctgttaatg cttcttcaat acgagcttgc tctgccaata aagattcatc
     1681 agaatgagtc gtctgacttt gatcaagggc agctaacaaa gcctttgaat cttcttgttt
     1741 ttcttgcaat tgctgataag ccatttgagc tctttgttca gctaagcgtg cctggtcacc
     1801 ggctaatttc aattgtgtca gttcttcttt ttttgctatc aaatcgcttt gcaaggcagc
     1861 tacttctttt tcagttattt ttaattgctc aactaatcgc gttaattcct cactgatttg
     1921 ttctaactct ggtttgataa aagttgtatt gctttgtcta ttagttcctc ctgaaaaaga
     1981 accacctggt cgtaattctg ttccatcaag agtaacaatt cttaccttat atcctaataa
     2041 gcgcgctgca atattagctt gatcaatagt ttcaaagata gcagtactag ataaaaggtt
     2101 ttggataata gctgaaagac tatcatcata gcgaaccaat gattctgcag ttcccagata
     2161 accttcacaa gtcgctaatt gacgatgata gtgttctgat aatgatctag cctttatagt
     2221 agttaaaggt aaaaaagttg ctcgtccttg gcgatttttt ttcaaataag caattgctct
     2281 ttttgctgca gcttcgtctg ttacaataat atgctgacta ttagcaccta atgcaatttc
     2341 aagagctgtt tggtaatcag aatcaaaaga aaggtgttca ctgactgcac ctatgatccc
     2401 tccaagcttt ttttgtgatt gaaggacagc tcgtactccc gcatagaatt gactatgact
     2461 tttttgaata gattctaagc tagccttacg agcttctttt cctttttttt gatccaacaa
     2521 gtcaaatagt ctttcttgct tcagttggta atctctttct aattcctgaa cgcgtttatc
     2581 accttcttga taattttgga gcaacatttc gacttgctct ttttgagctt tataatgagc
     2641 ttgagctttt tgagactcgt cattaagttg atcaagctta gtaacaagca gctgatattc
     2701 ttgagcctta tgttgacgag cctgtttttc tttgtctaaa tgggctttta aagctgtcag
     2761 ctgatttgat aaggccgcct ctttttgcat caataataca aactcttctc ttagagtttc
     2821 cattaattgg tcgggatcac ttgaaaaacg ctctaatgca ttgctcaatt cgtttaactg
     2881 ttgtttgaca tcacaaagtt gttgatcaat atgcaataat tgttctgtac gctgcttttc
     2941 ttctgcttga aaaccatcca attgttcttg taactgttcg aggtgttttt tggcttctgc
     3001 ctttttctct gcttcttggc cactttctag cttaaccaat tcaatttgct tttccaagtc
     3061 agctattagc ttggttaatt ctaataatgt tgtttgggtt tgatctgact cttgactaag
     3121 aacctgcttt ttttgcttga atttttggta atcttcttcc atagattgac gcttagcgta
     3181 ataagatgct aagtcttgtt gtaaagcagc taaagcttca gtatctttag tctgtctttc
     3241 ttgagctata tcaatatctt tgactagaat atctagttgt agttgcttac gattggcatc
     3301 aagttctaaa aattgtttag ctactttagc ttgcttttca agtggggcca actgcgtgtc
     3361 tagctcataa ataatatcct ctagacggtc taaattatct tgagtttgat ttaatttaat
     3421 ctgagtttct tttttacgtg ttttatattt taaaacacca gctgcttctt caaaaatggc
     3481 gcgcctttct tcaggtttac tattgaaaat ttcctctacg cgaccttgag aaataatgga
     3541 gaaagaatca cgaccaagcc ctgtatccat aaacaaatca tgaatatcac ggagccttac
     3601 cttgcgacca tcaatcagat agtcactatc tccatttcgg taaatatgcc tttcgacacg
     3661 gatttctttc ttggcagttt ttataaaatg atcggaatta tcaagaacaa ccgctacctt
     3721 agcatagttt agaggattac ggttttgtgt cccagcaaaa atgacatctg gcatcttgcc
     3781 cccacgcaga tttttagcac ttgattctcc taaagcccaa cgtaaacttt ctgtaatatt
     3841 acttttacct gaaccatttg gtcctacgac tgcagttact cccttataaa attcaatttt
     3901 agtcttgtcc gcaaaggact tgaacccttc caactcaatt tcttttaaaa acatctaaac
     3961 ctcactaagt tgggcaaggg catttttagc agcatcttgc tccgctaatt ttttagactt
     4021 acctaaacct ttactaagga cagcaccatt aacaactatc gatacctcaa attgtttagc
     4081 gtgggcaggt cctttctcac taatgacttg ataatcaata gccacatcac cttttgtttg
     4141 caaaaactct tgaagacaag ttttatagtc tctaaccctt tcaaaattcc ctttttccac
     4201 ctgagggatc atgacttgtt tcaagaagcg acgtactgca tcaatcccct tatctaataa
     4261 aagagcacct aaaaaggctt caaataagtc tcctaaaata gtatcacgac ggcgaccacc
     4321 tgacttttcc tcaccttttc ctagtttgat ataagcgtca aaagaacaaa atcgtgaaaa
     4381 acctgccaag ctttcctcac gtacaatcat ggaacgtaac ttagacatat ctccttctgt
     4441 ctttttagga tactttgcaa ataaatactc tgaaataatc aattgtagaa cagcgtctcc
     4501 taaaaattcc aagcgttcat tatgtgaaac gtttaggaga cgatgctcgt tagcgtaaga
     4561 ggtgtgggta aaagctgttt ctagtaaagt caaatcatta aattgaatat caaaagatgt
     4621 tgagagtaac tcttcaagct gtttcataaa aaatcccttt catatttgtc agtagacctt
     4681 atttagtata ccaaaaatcc acctagaaaa ccgactctaa gtggattttt cgtgatttat
     4741 taaacatctt aaatacccgt caaaggaaac tttctcatac aatcaaaaga ctctacacta
     4801 tctgagggga atttcccact acagatagta tagagctatt ttgtttttag tctgaacaac
     4861 taaaaaacta ccttatcttt ataatcctca acacctttga taaatagatc agacaagcca
     4921 aaactacagg ctaatttaag tctattgatg ctctaaagga tgaccaatga gagttcattg
     4981 aactcagaag acaatacgct ttaagacatc aaaaaaatag ccctgactgg tatatcacca
     5041 atcagcgcta ctttgcatat tctcttttag tttcttatct caaatatcag ttaatggaca
     5101 agcagtatct ggagaggtat catggaccgt aaagtctgta cctactgcta aatctgccat
     5161 agccagttga ttgaccatcg tcatatgcgc taactctttg atgttatttt ctttactcag
     5221 atgacctagg tatatttttt tggtattgta ccccaggctt ctaatcatgg ctcccgctcc
     5281 atcttcattt gataaatgtc ctaaatcaga taaaatacgc tgcttcaagc tccaaggata
     5341 agagcctgat ctcaaaattt caatatcatg attggattca atcaagtagc catctgcatt
     5401 ttcaataata cctgtcatcc tatcactgac atatcctgta tctgtcaaca tcacaaaaga
     5461 cttattatct ttcataatcc gataaaattg aggatcaata gcgtcatggc tgactccaaa
     5521 actttcgata tcaacatctc caaaggtcaa aactttatcc ctttggaaaa tatgtttttg
     5581 tgaggcatca agcttaccta gcatattgca ctcatccatc agctgccatg ttttttcatt
     5641 agcatagata tccaaatggt atttgcgggc cataacgcct acacccttaa tatggtcaga
     5701 gtgctcatgg gtaatcaaaa tagcatccaa atcctcaggt ttgcggtcaa tttcagcaag
     5761 aagactggta attttcttac cagtaagccc tgcatcaatc aataagcgtt ttttaggtgt
     5821 ttctaaataa aaacaatttc ctgtggaccc agatgctaaa atactatatt taaaaccact
     5881 ctcattcatt gattagtcta catcttcctc ccattcttca tagatggctg catctttttc
     5941 ataaggcaag acaatagtaa aggtcgatcc tttaccatag tcactcttag cccagataaa
     6001 gccatggtgc tgcttgatga tttctttagc aatggcaagg cctagaccgg tccctccttg
     6061 ggcacgactt cttgccttgt ctacacgata gaaccgatca aaaataagag gcaaatctgt
     6121 tttagggata cctagtcctt ggtctgaaat ggaaataatt aattgggtat ctgttgtttt
     6181 catacggact gtgatttttc caccatctgg agaatactta atggcattgt tcaaaatatt
     6241 ctcgataacc tgtgtcattt tatcattatc aatttcaatc cagacagaga taatagggta
     6301 atctcttaca atttcataga cttttcctgt acctgtatgc tgatttttaa ccaaatcaaa
     6361 tctgtttaaa atagaagtta taaaagcagt aaaattagtc atctctactg ctaattgggt
     6421 tacttgatta tcaatccgag acaggtttaa aagatctgaa atcatacgca tcatccgatt
     6481 agtttcatca agagaaactt ttatgaaact tggagcaata tcttctttaa gtgcaccttc
     6541 atcaagagcc tccaagtagg atttaaccga agttaaaggg gtccttaatt catgacttac
     6601 attagagaca aaaagacggc gttcacgttc ttctttttct tgttctgtgg tgtcatggag
     6661 caccacaacc aagcccgaaa taaaaccact ctctctccta ttcaacgcaa agcgcaagcg
     6721 taatgagaca aactcaccca tatcatttcg gctattaaca gttaccactg gtgttttaga
     6781 taccaaatca cggtaggtat atgaagtatc accttctaac aaatctgtaa tgtttttctt
     6841 tagtgcctct tctttactta aatttaattg cttgcgagct gtctcgttaa tcatgatgat
     6901 tttaccagac cggtctgtag caagtacacc atctgacata taagccaaaa tacttgccaa
     6961 gcgatttttt tcttgggcaa gattttcatg cgtcaagcga aaaacatctg ataggtcatt
     7021 taactggtct gaaagctcca ttaactcaat gtctgctttt tcgtcgatta tatcagtata
     7081 gcgaccatta atcaagtctc ggattttatg gctcatcatc cgaataatac gtgcatttcg
     7141 gtaatcacgc accgcaagat ggataaagta aaaagcaaca aaaacaagta agataaggat
     7201 cgctaattca aatgtggata agtttccaat gatatctcta gtcataagat ttcatgtagt
     7261 acccaacacc acgtcttgtt aaaatatact caggacgact tggtgtgtct tcaatttttt
     7321 cacggagacg acgaacagtc acatcaaccg tgcgcacatc gccaaaataa tcatatcccc
     7381 aaacaatttc caataagtgt tctcgtgtca tcacctgacc catatggtta gctagatgat
     7441 gcaatagttc aaattcacga tgggtcaact ctacctcttg accatgtttt ttagcaacaa
     7501 acgcatctgg taaaatctgt aaattaccaa tggttagttc ctgtgtacct gaagaagcat
     7561 tttcttctgc aacagccgtt tcaatagttt cggtacgacg cagatgagcc ttgacacgtg
     7621 ccagcaattc ccgattagaa aagggcttgg tcacgtaatc atcagcccca atttcaagtc
     7681 caataacctt gtcaaactca ctatctttag ccgacaacat aataatcggg acatgactgg
     7741 ttttacgaat ttccttggct acttcaagac cgtccaactc agggagcatc aaatcaagaa
     7801 taattaaatc tggcttttct tcttcaaaaa ttgttaccgc ttcgcgtcca tcaaaagctg
     7861 taacaatgtc ataaccttct tttgtcaaat taaacttaat aatgtcagaa atcggttttt
     7921 catcatccac aataagtatt tttttcatgt ctatacctca gaaagttctt gtaattgata
     7981 ttacaatttg tgatttttgt ggttaataaa aaatcacaaa actatatttt tctaaattat
     8041 ttctacctga ttataccaaa aattcagcct aattgacaat gacgactacc gttacaaata
     8101 gagtactttc taaaaccaac cctttatagg atgaatttga cgtttgaatt gcttagcaga
     8161 ttttttgact ctggcaaaac tactcttgtt gtggtaagca aaaagtcctt tggcattaaa
     8221 ataagagaac ataaagtcat ctttttgagc tctaaaccac tgacgaaata ctctttttgg
     8281 cgatcgaaat cctttaaggg tgtcccttct aaactgagga tcgtgacggc taatcggatc
     8341 aacatctaaa tcccaatgtc taggaccatc tgtgccatag ttaaaagatg caccattgac
     8401 aattcctttt tcggtcacta cttctgcatc aacttcatta agaaaaaagc ctgtgctatc
     8461 tagaataaat tcggtatgaa accaaagaag tacttttata ttataggaaa cttcttgact
     8521 tggaaaaatg actttattat cttcaaatct tagtttatta tgtaacctgg cagaatccat
     8581 caaggtataa tctggtgctt gccgccacaa acaaggccct tttttagacc tcaaaaaaag
     8641 ggccagagct tgggcgtctg tcatctcaat cattttataa tggtttctga tagattgagc
     8701 ttgttgacta gagatgacat aacgaaattg atgcaaacgc ttttcaagct cacctgcttg
     8761 gcttagtgtt ttgccaggaa aagcttgatc aactaagcta gccaactctt tccaaaacat
     8821 atgatctggc aatagagatg gatggaatcg ggtgagtagc gtttgagttt catgcaactg
     8881 accacttagc tcaaatggca tctctaccaa atcaagaaga aacgttagta actgcttagc
     8941 tgattggttt tctttttcaa taggccatat tacccagaaa gttggagaac caagtcgatt
     9001 atcaatgata ggctctttag ctatagcacg aaatgcttgg tttgataaac ctgcgttttg
     9061 atagcatttc aaggcctgat agcgatctaa gatagcttgt ttcatggcct tctcctcatc
     9121 ttttttgacc atattataac acatctaaag ctgaatcgtc acttgtatct cactataagc
     9181 aaaacgaaac ctgaagtggt taactaagcc actgtttggg tattttattt aaaagaactt
     9241 ctcgattaat tttaccatta tcttttaaag gcaaacaatc aatcacaaga tagtacttgg
     9301 gaagttgtcc agagtttaaa taaagagcta aaaagtcttt aataggtgct aggcaatcct
     9361 tcttagtcaa aactaatact aatattaggg tttcgttttc tccttttcct atctttaaag
     9421 cgtgcgcttc tttgacgttt ggcgcttggt gagcgagttc aactaggcta ggcaggtggc
     9481 atttcactcc tcgttgatta acccagtcat cttgtcgccc ttctaaaatt aaacccgctg
     9541 ggctcatttt tcctaagtct gagacactgt atggttgtga cattccttct acactataag
     9601 gagtctctac aaaaatttcc ttatctttga aactgataga cacatcagga aagggctgtc
     9661 ctaccaaacc attaattttg acagcagctc tgccattaca ccacgttata aaagataatt
     9721 cactagcccc gtagaaaatg acaatttcaa gctgaggaaa ttttttgtaa taatgtctta
     9781 gtaattcttg tgatatcatt tgcgaagagg tcaataagtg agtcgcagtc atgttgttct
     9841 tagtcaaata aggtagcaag cgatttaggt aggtcggtaa aaggtaaagg tgactcacct
     9901 tttttgcttg ccataagctt aaccacgttt tcaaagacag cttttggctt aaaaccagac
     9961 atcccccggc ccatagttga gctaatgcta agttaagatt gccagtaaaa ctaaaactac
    10021 catgtaagaa tagacaacta ttaccagtca taccaaacac cttattttga taatcaaaaa
    10081 gtctcgtcca agttgataga caacgccaaa ataattttgc tttgccagtt gttcccgaac
    10141 tcaaaacagc aaaatcagct tttttaagaa gctcactatc gacgtgtttt atttgctgct
    10201 gaaatgtacc tgagatatta gggtgtaaaa tgataggaca actgccttct tttaatcctc
    10261 gtaaaaaaga gagtaactga ttaagatagc gactatgact gatgatatat ggtacactgt
    10321 cttttattgt ctgatccttt attaaaaccg cttgccatag ctcttgataa gtcaagctta
    10381 tctgatcggc tacgatagct tttttatttg ggcattgctt tgcccaatat tctagctttg
    10441 tcagcatgac taaactcctt atgatatttt agtaaaacag caaacccttg gccaccagct
    10501 gctgctatag cagctattcc atagcgtcca tttttaatct ctaaagccct cattaagtgc
    10561 aatattataa tggctgctga ggcaccatag ggatgaccat aagctaatgc accaccaaat
    10621 atattatagc ggtccaataa atctggataa tgggtttcaa atagaccatc aataacggca
    10681 aaggcttcgt tccattcaat cgctgtcata tctgccatcc ctagaccatg tttttccaaa
    10741 aggacttgac tagccttaat caccattaaa gggctacgct gtgggtctcc tgctacttct
    10801 accacatcaa tcagtttaaa agctgaaggc tgactactta gctgtaaaaa ggcagcagca
    10861 tcatgggtta ggcaggcatt ggctgcacta atcacgtgat cttctcctaa gatagggggt
    10921 acctttgaca acaccttact tgacaagcgc ggtcgaatac cttgatcact ggctcctggc
    10981 aggtctagaa taagatcagc taacagggct tgctcctggc aataactagc cttttgatga
    11041 cttctcaatg tccagtgatt caaatactcc tttgtaaagc catgctctct tgccactcgt
    11101 tctgcccctt caatcatcgc aaaagggctg atactatctg gagaaaactg agctaccttg
    11161 taggctcctt ggcgccaatc agctgaagca tataccgatt caggttgtaa ggagctgctt
    11221 tcaatccctc ccactaaaag gttgcttgcc atacctgcct tgattttagc ataacccacg
    11281 gaaagagctg caccagctga tgcacactgc atatcaaccg tcaaagcaga gacagattct
    11341 cctaaatgag aatacagggt catcaggcgc ccaatattac cacccgttcc gacggcattg
    11401 ccacaaatca cttggtcaat gggaactgga tacttatttt gtaaagcatt gatgagcttt
    11461 gctccaagga tttctggttg ttctttagca aattgtttcc ctactaaacc gataggggtt
    11521 ctaaggcctg cagcaatata aacatccgtc attatattga cctcctttat tttcgatgtc
    11581 cttaaatggt tattatataa ttatttctga tggttttctt ttttgaacta ataactgcta
    11641 aaaatacacc taaattaatt tagagaatct ctttagtctc gctagtgtgc catcaataaa
    11701 atctataaaa ttctctaacg tcattgaaat gtcaagtccc acaactattg tcgtagaagt
    11761 cacttacttt gttgctttaa cattcaaact gtaacctttg tttttgatac agggtgactt
    11821 tattatactg gaaaaccatt cctaagaaaa gaaaatctta tatagaataa agaattagac
    11881 accataaaaa agctaagctg tgacacttaa cttttttatg gtattgacaa accttgtctt
    11941 tactatttat tgatagttaa tgcctctaac taaaataaaa gccatgtctg tatcagcctg
    12001 caaggcatct gctaagctgg tatcgttact agatttgaca ggtctcataa aggcaaaaga
    12061 tctcaaagtt ccataaacgt tatctagcaa ttgcatgcaa ccacttgaaa tatatctgct
    12121 tatgttattt taccgccctt aatttagacc aatctgctga tgatggttcc aaacaatgcc
    12181 tctaggaacc tgcactttca taagcatcta ggccccattt ccaaagctta agcgacaagg
    12241 ccaaaaccag taacgacacc actaccaagc ctccaatatt aaagagcagg tgctgacctg
    12301 ttaaaaaata agaagcaggg taataagctg taaaagcaaa gggaatgaca aaactgatta
    12361 accaccgcaa aaaggaatgg taaatggaca tagggtactt ggaaaagtca ttaaacatgt
    12421 aaaaaatata aataactgcc ccagactgct tggtccaaaa agaaatgctg gcggtcgcaa
    12481 tttttaaaga ggtgtaaatc aaggtcgcaa aaggaataac caaaataaac aaaagcacct
    12541 taggaagagt ccatacgata ctaccagccg tggtcactaa taagataacc ccaaccaata
    12601 attcacccag ggcatccact tgaaatgtct caaccaaaac atgaaacaaa ggactgattg
    12661 gtctggtgag gtacttgtca aattctcctt tgcggacaag ccgttgccct aaagcccaaa
    12721 gattgtcaaa aaagaggtga tcaattccct ttggaatcaa agaaaagcca taaataaagg
    12781 caatttgttc aaacgtccag ccttctaaag aagggatatg ttggaaaagt accgataaaa
    12841 agagcagatt aagtccctga gtgagaaaaa cacctagcac tcccaccaca aaatcaacct
    12901 tgtattccat aatttgtttg aggtattgtt tgataaaaat agcgtgcata cgtctcaatt
    12961 ttgccatctc accctccttg aatcgttaaa tggtactgaa cctttttcca gatgacttgc
    13021 gataagacta ccatcaccag taaccaaaag atttgcaaac ttagggccac cattatctga
    13081 ctaaggctgt atttcccaat gacaatcata actggcgtgt aaaccaaaga ggaaaacggt
    13141 agaaaactaa gcactatata aaccacttta ggaaaaaaag ccaatggaat caaactgcct
    13201 gacataaagg caaccaaggc atttttcaat aaattagacc cccaaaggtt tttaaagaca
    13261 aatgctgaaa agccaaaaca aatgttgaaa taaaaattga ttaaaaaagc taataaaagg
    13321 ctaactaaat acagacaact gctggctagg acttgcaagg tagacagccc cgccatgact
    13381 ttaataccag ataagaccat taaaaaagga aagccgacac tcattagcac aatccagcga
    13441 aaaccaattt ccataaagag gtaagaggct gcaaaatgaa ctggtcgaag caggcgcatg
    13501 atgatagaac catctttcac ttcttcgcca atcataaagg agctgtctga tttggtcagt
    13561 aaagtggtca caaaactcat gataatgtaa aaggtcatgt cagacagagt aaaaccattg
    13621 attaaggatt gcttagacga atcaaaaaca gccttccaga ggtaataagc cacaaaagcc
    13681 cccatgacat ctccaatacg atacaaaaag aaattaacgc gataggttat taattcctga
    13741 attcctgcac tgagaaaggg tctataacgt ttccaaaatg accacatctt aaagctcctt
    13801 tcggtagaaa cgacgaacaa tatcctcaat atctacatca gtcatcttca aatccctgac
    13861 cgcaaaatca gccatggttt tttgaataat gtccgctgtt tgatagcgcg aactgtcata
    13921 ttgaatgtct aaactaagct catgccgttc cactgtgatg tcaggtaagc ccataaactg
    13981 tgagaccacc tgctcctgcc ctggcttcaa ctcaaaagac agactcttca tttttccaaa
    14041 gctttgcttt aattgggtca ccgttccatc aaagatttct tgccctttat caatcatgat
    14101 aatccgatca cagagctgtt caatatcact caaatcatgg gtcgtcagta aaatagttgt
    14161 cttttcttct tgattaattt gagtaatggc acgccgaatg ttgtccttca cagaaacatc
    14221 aagtccaatc gttggttcat ctaaaaacaa tactttagga ttatgcaaca aggaagcagc
    14281 aatatcagct cgcatccgct gaccgagtga taaggtccgg acaggatcct taataaactc
    14341 attcaggtcc aaaacctcat ttaaaaaatc catgcgcttg cgaaaggctt tttcaggcac
    14401 atcgtaaatt tcttttaaaa cgacataggt ctcttgcaag gccaaatccc accatagttg
    14461 tgttcgttga ccaaaaacag ctccaatgtc tctcacataa tcttgacgat tatcttgtgg
    14521 aatctttccg ttaatgcgac agtaacctga tgtaggcttt aaaatacctg ttaacatctt
    14581 aatcgttgtt gattttcctg caccattggc tccaataaaa cctaaaattt gccctttggg
    14641 tacctcaaaa gataagtcct taaccgcctc aaagatttct ctttggggat gaacaaagga
    14701 cttcaaggct ccctttaatc ctggctcttt aatggttttg gaaaaatttt tttgaaggtg
    14761 ggacacttct atcatcacca tttaccattc ctcgtttctg ttatcgtctt atcaccgtta
    14821 ctaaacaatt tattataaca ccatgaaaaa gactgtgcaa ccgtttgctt aattttttaa
    14881 gaaagaaaaa gaagcccata aggacctctt tttgccattg attaagcttg tgcatctgga
    14941 catgacttgc gagcaatgtc tgccaagatg ttttccacaa attcttccac agattcagtg
    15001 tgagtcgcct tactaccata gcggcgaaca ttgactgatt tgtcttccat ttctttgtcc
    15061 ccaacaatta attggtaagg aattttgctg gtttgagacg cacgaatctt gtactgcatt
    15121 ttttcattgc ggtcatcgac atctgcacga acaccacggt cacgaagggt ttttgctact
    15181 tcccaagcat agtcaatgtg ggcttcattt gaaattggaa tcacggttac ttggtgaggt
    15241 gccaaccaag ttgggaaagc acccttgtaa gtttcaataa ggatagcagt gaaacgttcc
    15301 atagttgaga taacaccacg gtgaatcatg actgggcggt gttcttcgcc atctgcacca
    15361 atgtatttaa ggtcaaagcg ttctggcaac aagaagtcca actgaatggt tgatagggtt
    15421 tcttcgttac caagagctgt tttcacctga atatcaagct taggcccata aaaggctgct
    15481 tcaccttctg cttcaaagta atctacaccc atctcatcaa gtgcagcttt aagcatactt
    15541 tgagcatttt cccacatctc atcattgtca taatatttac gagtatcgtt agggtctcgg
    15601 tatgacaaac gaaaacgata atcagtcaag ttgaagtcag cataaacatc aatgattaat
    15661 tgaagggcac gttgaaattc ttcctggatt tgctctggtg ttacgaaaat gtggccatca
    15721 ttcaaggtca tttcacgtac ccgttgaagt cctgataggg cacctgattt ttcgtaacgg
    15781 tgcatcatgc caagctcggc aatacgaata ggcaattcac gataagaacg cacatgattt
    15841 ttatacactt gaatgtggtg tgggcagttc attggacgaa gaacaaactc ctcaccatct
    15901 cccatatcca ttacagggaa catatcttct tgataatgat cccaatgacc tgatgtcttg
    15961 tacaattcta cagaagcaag tggtggtgtg taaacgtgtt ggtagcctga tgctaattct
    16021 ttatctgtga tgtagcgctc aagtgtacga cgaatagtcg ctccatctgg taaccaaaac
    16081 ggcaagcctt gaccaacttc ttggctaatc atgaacaagt caagctcttt accaagctta
    16141 cggtgatcac gttctttggc ttcttctaga cgagtcaagt aagctttgag gtctttttta
    16201 tcaaaccaag ctgttccata gatacgttgc atcatgttat tgtcgctgtt accacgccag
    16261 taagcacccg ccacatttag caagtggaag acttggatac ggcctgttga tggcacatga
    16321 gggccacggc aaagatccac aaattcacct tgacggtaaa ccgtcaagcc agcaccagca
    16381 tgctcattaa tcaattctac cttgtaagga tcatctttga acaattccaa agcttcttct
    16441 ttagtcactt cttcacggat acatgggtaa ttttctgtca cgattttttg catttcagct
    16501 tcgatacgtg gcaaatcttc gtttgaaatt tggccttcag cattatctgt atcatagtaa
    16561 aagccttctg caatggcagg gcccacaccc aaatgaagat ttgggaacaa gcgttttgca
    16621 gcttgggcaa ataaatgggc tgcagagtgg cgaagcacct cataagcatc tttgtggtca
    16681 ggtgtcacga tttcaatact gccatcttct tcaatggcac gagtggtgtc aatgagttga
    16741 tcattgaact taccagctaa agctttttta gccaatgatt tgctgatgga ctcagcaatg
    16801 tcaaacgttg ttacaccaga ttcaaattca cgcacggcgc catctggaaa agtaatctta
    16861 atcatttgct ttctccttta agtcagttaa gatttccact aaaacatgga tagtaagaca
    16921 aattagggac aagaggttag tgaatagctc aataccacca cgtagaagaa caatacctta
    16981 ttcacgaatc taccctccct agtccttaaa aacaaggcta atcccctcca aaacaaaaaa
    17041 actgaccgca tcccaaagga cgcaatcagc gtggttccac cttaatttat gcagggcaaa
    17101 aaacatgcct tgcacctcta gtggcgataa gggggccacc cttttatgtt tgcataaaaa
    17161 ccaggaagga gtatcagatt agctatcctt gtgctctttc caccaaccag cactcgctta
    17221 aaagattctg tccaatctaa cttgtcttca agataattat agcaagtttt caaaaattta
    17281 caagtcttaa tccagaaaat ctttgatagc atggaccagc ttgataggag ccttgactac
    17341 ttttactgag gtgatagctg tgaccttagc agcacgttta ggcaagtgaa tagcaccttg
    17401 cactaatttt agagaactac ttttaccaga atgattaaga taaagactca gcttttgagc
    17461 ttctttgcta tttctagcaa ttaacgtatc taaatagaac gtgtaaatag attttccaaa
    17521 gtgctgtgct gagatttcat aacgtttttt tgctaataac cgtttatcca taactggtgt
    17581 ttttagtatg gcatcaataa tagcatcagt taaatctgtt tcagcgtaat aaagagtgcc
    17641 aaacatttta tcagtcacca catcatctaa ataaggattg ccatgagcaa taataggagt
    17701 gccactagcc aaactttcaa tataggtcaa gccctgagtc tcactggtcg atgctgagat
    17761 aaagaaatca caagccttat agtacagagc aaccttatca tgaggcacca tgcctgtaaa
    17821 ggtcacgtgt ttgtcaacct ctaactgcat cgccaagtgt ttcaaatctt gcaaataggg
    17881 gccatctcct acaataataa gctttatctt ggcattttca gccaaaatag ctggcatctg
    17941 attgatgata gcttgaatat ttttttcata agaaatccgt gacaaactca ataacatggt
    18001 ttcatcacca gcaatgccca attctgcttt taagttggtt acttcttctg ctgtgatgtc
    18061 atcacgaata tatttttcca aaggaatgcc tgttgggata acccgcttag ggatagtaac
    18121 ttcgtaacct tctagaagat tgaggacaat cctacttggg cagataaccc catccaaatc
    18181 cttcaaatag cccctaagaa gaggtttgac catacttggt cgaatgattt ttccgttggc
    18241 aatataactc acgtagtcct catattgggt atggtaagta tggacaacag gaattcgcaa
    18301 agctttgcct atcattttcc ctaacaagcc taagctaaac tcagtttgcg tatgaataat
    18361 atcaagatta tagtgttttg caattttgta tgacgaaatg aggccacgat aaaccacacg
    18421 tctatccgta aatgacacaa aaggaacact tggcagtcga ataatggtcg ggtcttcaaa
    18481 gcgtttgaca tctctatcag tagtggtgaa aatataaact tcgtgacctt ctttttctag
    18541 ctcttctttt aacgtacgaa tactagtagc gactcctgaa acttgtggaa aataggtatc
    18601 tgtgaataga cctacacgca tcttataact ccattacttt tttatagaca tctactaagg
    18661 catggccaac cgtttctagg cgacgactct gggcaacctt gtaaccagct tcaactttgt
    18721 tgctggcacc agaaaagact tttttcagag cttctataaa acctggtata tcggttgcta
    18781 attccgcact actttgatca acccatccgt agtaaactgg tatatcacgt aaaacaaggt
    18841 gctgacgact ggccaaggct tctaagacaa caatgccttc tgtttcttca cgacttggaa
    18901 agaaaaaggc atctgcacca gtcatggcac cttcataaac atccccttta atgtatcctg
    18961 ggaaaataag atttttcggg tggttaccat tgaccatttg gcgaacttga gcaggaatga
    19021 cccatttgtt ggtctcgcca aaccagataa aacgaacatc tggcatagct tgggcaactt
    19081 tgacaaagtc atcaattcct ttcctcagaa aaaataatcc tgctcccata accacttttt
    19141 caccctcttt aatgtcaaag tagcgacgaa aagctgcttc ctttttagaa tctgctccgt
    19201 actgctccaa atcaatccca tttgacactg caaaaatagg tgacttcact ccataactct
    19261 caatcaaaga cttagaatat agggtagggg taatgatagc atctgcctta ttgtaaaagt
    19321 gacaaaggta ttttttaaac caaggagata atagatttga aaaaataaaa gaatttctaa
    19381 aatcttcttc tgtagaatgc ccatgcatga taaccttctt accagctttt tgtgctttta
    19441 tcatcagcag ccaacttttt aaaccatagg tattgagatg aaccaaatca taagtttctc
    19501 ttggattagt cgtaaaatgt tgaccaacaa gtgacaaggc tttagcctga tgcttaatcg
    19561 ctcgaccaat tcctgatttt cttagataat tttctgcttc taaatacaat aagactttca
    19621 taagttctat tatagctcat tttatagtat ttttcactaa gacatgcaaa aatcagccct
    19681 aaggctgatt ttagtattga agaaacagtc tattgtgata ctttttgtta gatggtgctc
    19741 ataagtcaca gttacttagt tgtcccacgt tttttaatac catgattcaa aagaatttct
    19801 ttttcttcca actcttcttt gttcatgatt ttagtcaaca tccgcatgct aacagcacct
    19861 aagtcataaa caggttgact gatagaactc aaattgggac gagtgtattg aaccactggt
    19921 gaatcgttgc tggtgatgat ttcaaagtcc tctggtacgc gtttacctgc ttcaaacaag
    19981 ccgtttaaaa ggcctgctgc caattcatct tcagccacat aagccgctgt tgctcctgag
    20041 ttaatgacgc gctgcgccaa ttcaaatcct tctttataag aatagttcgc ttcaaaaaca
    20101 agaccttctt tgaagtcaag cttattgtgc ttcaaccctt ctttgtaacc tgctaagcgc
    20161 actttaccat tgatatcatc aatgagtggt cctgacacaa aagcgataca cttatgattt
    20221 tcagctaaaa tatcaacaac gtttgacacc gcagctctat agtcaatgtt gacgcttggt
    20281 aattgatgat caagatctac agttcctgcc aaaacaactg gagtacgaga gcgtgaaaac
    20341 tcggcccgga ttttttctgt caagtgatgc cccataaaaa taataccatc cacttgcttg
    20401 gcaaaaaggg tattgacaac attaacctcc ttatcatcat cttcatcact ggaggcaagc
    20461 acaatattat atttgtacat agcggcgata tcgtcaatac ccttagctaa gatagaaaaa
    20521 taactatttg caatatttgg aatcacaacg ccaacggttg ttgtcttttt gctggcgaga
    20581 ccacgcgcga cagcattggg acgatagtca aggcgatcaa taacttctaa aactttctta
    20641 cgtgtatttt ccttaacatt tttattgcca ttaacaacac gactaacggt tgccattgaa
    20701 acaccagctt cacgggcaac atcataaatt gtaatggtat catctgtatt cattttttat
    20761 cttcctttct atttgaaaat gacgctttca aaaagtattt tagcatgatt tgtaaccact
    20821 ttcaagtgtt ataggttaat ttttgaaaaa gttttcaaaa attaactcga agcttgattt
    20881 tttaagcttt ttttgaaaaa atagtaagta gaaagaaggt tagcctatga caaaattaga
    20941 ccaaatacgt ttgtatcttg accaaaaggg agctgaattg gcgattttct cagatcctgt
    21001 tacgattaac tatttaactg gattcttctg tgacccacat gaaagacaac tgttcttgtt
    21061 tgtctaccat gatttagctc ctgtgctttt tgtacctgcc cttgaggtgg ccagagctag
    21121 ccaggccata tcatttcctg tgtttggtta tgttgattct gaaaatcctt gggaaaagat
    21181 caaggccgtt ttaccaaata cagcagccaa aaccatttat gttgaatttg accaccttaa
    21241 cgtcaataag ttccatggct tgcagactat tttttcagga caatttaaca atctaacccc
    21301 ttatgtccaa ggcatgcgcc tcgtcaaatc agctgatgaa atcaataaaa tgatgattgc
    21361 cgggcagttc gctgataagg ctgttcaagt tggttttgac aatatttcct tagatgctac
    21421 cgaaacagac gtcatcgctc aaattgagtt tgaaatgaaa aaacaaggca ttcataaaat
    21481 gagttttgac actatggtct taactggaaa taatgcagcc aatccccatg gcattcctgg
    21541 aacaaataac attgaaaaca atgcccttct tctttttgac ttaggtgtcg agaccttagg
    21601 ctacaccagt gatatgacca gaactgtggc tgttggtcag ccagaccaat ttaaaatcga
    21661 tatctacaat ctctgcctag aggcacaact agcggctatt gactttatca aaccaggtgt
    21721 aacagctgct caagtagatg ctgctgctcg tcaagtgatt gaaaaggctg gatatggtga
    21781 gtacttcaac caccgcttgg gacatggtat tggcatggat gtccacgaat ttccctccat
    21841 catggctggc aatgacttgg tccttgaaga aggcatgtgc ttctctgttg aacctggtat
    21901 ttacattcca ggaaaggtcg gtgtgcgcat cgaagactgt ggccatgtca ctaaaaatgg
    21961 ctttgaagtc tttacccata ctccaaaaga gttgctttat tttgaaggct aagcccctat
    22021 catctggtta aacaaaccct cgcgaaagga aggtattcgt atggatcaaa ccattcatca
    22081 ccaaatacag caagcactac actttagaac agccgttcgt gtttataagg aagaaaagat
    22141 ttctgatgag gatttagccc ttatccttga tgctgcttgg ttaagtcctt cttctgttgg
    22201 cttagaaggc tggcgctttg tcgttttaga caacaagcct attaaagaag aaatcaagcc
    22261 ctttgcctgg ggggcccagt atcaactgga gacagctagt cactttattc ttttaatagc
    22321 cgaaaaacat gcaagatacg atagccctgc tatcaaaaat agccttttac ggcgtggtat
    22381 caaagaaggt gatggtctca acagccgcct aaaactctat gaatctttcc aaaaagagga
    22441 catggatatg gcagataatc ctcgggcact ctttgattgg acagctaaac aaacctatat
    22501 cgctcttggt aatatgatga tgacagctgc tcttttgggc attgatactt gccctattga
    22561 aggctttcat tatgataagg tcaatcatat cctagctaag cataatgtga ttgatttaga
    22621 aaaagaaggc attgctagca tgttatccct aggctatcgc ctgcgagatc ccaaacatgc
    22681 tcaagttcgt aagcctaaag aagaagtgat ttcagtcgtt aaataaggag attaaaatga
    22741 aagcattaca tacctgtatc cgcgtcaaag accttgacca gtcggttgct ttttacacaa
    22801 gcgccttccc ttttaaggaa aactatcgta aagattttcc agatagtcaa tttactcttg
    22861 tttatctggc tttagaagga gaaagttatg aattagaatt aacctacaat tatggtcatg
    22921 gcgactacga cttaggaaat ggctatggtc atattgccct tggctctgaa cattttgaag
    22981 cagaccataa aaaacatcga caagcaggtt ttccagttac cgatatcaaa gaactggctg
    23041 acaaatctgc ccgctattat ttcattcagg atccagatgg ctacaaaatt gaagtcattg
    23101 atttaaacaa ctaatactat taacaacaga agctttaaag cttctgttgt tttatttttt
    23161 aacctgacga ttgggacaat ctcattatta acagagtttt gaaatatctc agcttttgtg
    23221 ttacaattgc cctaaaattc catttgaaag aaaagtctat gacactacta tctatcattg
    23281 tcccctgctt taatgaagaa gcaaacattc tcccttattt tgaagaaatg catcaactag
    23341 aaacgtccat gaccaaccaa cttgcttttg aatacatttt tattgatgat ggttctaagg
    23401 ataacacctt gggtattttg cgtgagctag ctgctcgctt tccaaatgtg cattatctgt
    23461 ctttttcacg ccattttggc aaggaagctg gactcttagc tggactaaaa gaagccaaag
    23521 gcaattacat tactgtgatg gatgtggatc tgcaagatcc acccgaactt ttgccagtta
    23581 tgtacgctaa gctaaaagag ggttatgaca tcgttggcac caggcgccaa aatagacaag
    23641 gagaaccact aattcgttca atgtgttcga atctatttta tggactgatc aaacacttat
    23701 cagatactga aatggtcaat ggagttcggg attatcggtt aatgaccaga caagtcgttg
    23761 atagcattct tgagttggga gaggttaatc gtttttcaaa agggatcttt tcgtgggttg
    23821 gttatcgtat cacctatctt agttttgaaa atcaaaaacg caaatatgga aaaagtcgtt
    23881 ggcatttttg ggagttgctc cgatattctt tagatggctt tatcaacttt tcagaaatgc
    23941 cgttaaccat tgctacctgg acagggactt tcagcttttt gatttctatt ttcgccatct
    24001 tatttatcat tatcagaaag atactctttg gtgatcctgt ctctggttgg gccagcactg
    24061 tctctattat tttattcatg ggaggcattc agctcttttg catgggtatt attggaaaat
    24121 atatttcaaa aatattcctt gaaactaaaa aaaggcctct ctatattatc aaagaaaaac
    24181 attaaattaa aacatttttc tcatcaatcg tttcctactc cttctgtgcg cgaagctttc
    24241 tttttaagcc cgttatacca aatgataatg acctttccaa aatagtatca ctttccttaa
    24301 cactttaagt agaaaaggaa ctattaggtt gagccttttt gcaaatccta gcgactaatg
    24361 ttataatcgt catactattg aaaaaggagt tctttatgga gtggattaaa ttaatcggta
    24421 ttgtgattat cgtattaggg tttattttaa aatttgatgc tatcgctacc gtagttgttg
    24481 caggacttgt taccgcgctg gtttcaggaa tttcgtttat tgatttttta gatattttag
    24541 gaaaagaatt taccaatcaa cgtttgctaa ccattttctt catcacttta ccgcttatcg
    24601 gcttgtcaga aacttatggg ctgaaacacc gagctactca gttgattcaa cgtgttcaag
    24661 cccttaccgt cggccgttta ttgactctct accttattat tcgagaatta gcaggtctct
    24721 tttcgattcg actaggggga catccacagt tcgttcgccc cttgattcaa cctatgggag
    24781 aagctgctgc taaagccaac attggagaag agttgaccga tgcagaaaaa gatgacatca
    24841 aagctatggc agctgccaat gaaaactttg gcaatttctt tgcccaaaat acctttgttg
    24901 gcgcaggagg tgtcctactt atcgctggaa ctttagaaca gcttggctat gatggtaacc
    24961 aagctaaaat tgcattttct tctattttaa ttgctattat ctctattatt atagttgcta
    25021 tttacaatta cctctttgag aaaaaaatgg aacgtcaaca tcagaaagga gataactaat
    25081 ggctaacatt gcaaaccttg ttttagaaac gatatatatc cttatcggct tgcaactgtt
    25141 tcacacagcc tactgcacct ttaaagataa aaccaaccct gtctattttg ggacagctct
    25201 gttctgggga ttgctgggtg ttaccttcgt tggcggtgcc tttttgccta ataaagttat
    25261 cggttttatc gtgattgtcc tagctctttt aaccttgttt aagcaagttc gtattggaac
    25321 attaccagcc tttaatgaac aaaaagctga agaaagtgct caccgcattg gtaactggat
    25381 tttcttacca gttatgttaa tggctatgat ttctctactt cttgccttga tccttcctga
    25441 tttcagtaaa agtgccattg ggattgctgg tatcttagcc actattgcta ttttaataat
    25501 cactaagcaa aaaccatctg ctcttttagc tgaaaataat cggatgaacc aacaagtgtc
    25561 cacaagcggt attttgccac agctcttagg agctttagga gctatttttg cagctgctgg
    25621 tgttggtgat gtgattgctt ctctcattcg ggagattgta cctgctgata gccgcttctt
    25681 tggtgttctc gcttatgtgc ttggcatggt tatttttact atgattatgg gaaacgcttt
    25741 tgctgccttt accgttatta caaccggtat cggtgttcct tttgtttttg ctttaggagc
    25801 tgaccctatt attgccggtg ctttagctat gactgctggt ttttgtggta ccttattaac
    25861 accaatggca gcaaacttta atgccttgcc tgttgctttg atggagatca aagatcgaaa
    25921 tgcagttatc aaaaagcaag ctcctatcgc ccttgtttta attattagtc acattgcctt
    25981 aatgtattta cttgcttatt attaactttt ttgctaagtt agtagcgtca gttattcatt
    26041 gaaaggacat tattatgaaa attcttgtaa caggctttga tccctttggc ggcgaagcta
    26101 ttaatcctgc ccttgaagct atcaagaaat tgccagcaac cattcatgga gcagaaatca
    26161 aatgtattga agttccaacg gtttttcaaa aatctgccga tgtgctccag cagcatatcg
    26221 aaagctttca acctgatgca gtcctttgta ttgggcaagc tggtggccgg actggactaa
    26281 cgccagaacg cgttgccatt aatcaagacg atgctcgcat tcctgataac gaagggaatc
    26341 agcctattga tacacctatt cgtgcagatg gtaaagcagc ttatttttca accttgccaa
    26401 tcaaagcgat ggttgctgcc attcatcagg ctgggcttcc tgcttctgtt tctaatacag
    26461 ctggtacctt tgtttgcaat catttgatgt atcaagccct ttacttagtg gataaatatt
    26521 gtccaaatgc caaagctggg tttatgcata ttccctttat gatggaacag gttgttgata
    26581 aacctaatac agctgccatg aacctcgatg atattacaag aggaattgag gctgctattt
    26641 ttgccattgt cgatttcaaa gatcgttccg atttaaaacg tgtaggaggc gctactcact
    26701 gaccgtgacg ctactaaacc tattttaaaa aaacagagat atgaactaac tctgtttttt
    26761 ttgtgctaaa aatgaaagac ctagggaaac ttttcatcgg tctttctcaa ttgtcatctt
    26821 aatctaatac tacttctaac atcagcgggt atagtttgcc agtaattaag aaacgttgtt
    26881 gatctaaatg agcaatccca ttcaaaacat taaggtcagg gtaatgggac ttatcaagat
    26941 ttaaggcttt taacaaagga ctaatatcat aggtggctac cacctttcca gaatcaggtt
    27001 ggagtttgac aatagtattg gtttgccaaa tattggcata gagataacca tctacatact
    27061 ctaattcgtt aagcattgag atggggacac tttctatagc aactagtacg gtatccaata
    27121 aagcaaaatc tttggggtct cgcttttgca aaaaagcatt tccactagtc atccaaagac
    27181 actggttttc cttatcatag gctaatcccc agccatctcc ttcaaatgga taggccccta
    27241 aacaattgca ggtagcctta tcaaacttgt aggctacacc ctctttatag gttagtaacc
    27301 aaaaataatc ctcaacaact gttagccctt ctgcaaaaac tgtatctgga aaagctattt
    27361 tttctgaaaa gatctcttga gtaaggtcat acacccctac ttttgagaaa ccataacgac
    27421 cggcactaag taatatatga ttgttgttga gttgttctaa tccctgcgtg tataagttgc
    27481 tatcataaga ataagttcgt aacaaacgaa cttgtccttt agttatcata atactcattc
    27541 accttacctt gcattgtagt gtttcatttg ttttttaata tctctttcct gatcacggcg
    27601 tttaatggtt tcgcgtttgt catactcgtg tttcccttta gctagaccaa tcagaacttt
    27661 agcaaagcca tccttgaggt aaactttgag aggaaccaaa gtcataccag atcctttaag
    27721 ttcatttgct agatgcgtaa tctcacgttt tttgagcaac agtttgcggg tccgttcagg
    27781 atcagcgttc caaatattac cttgttcaaa aggtgcaata tgcacgttga ccaaccacgc
    27841 ttccccattc ttaatttggg caaagccatc tttcagttga atacgagctg cacgaacact
    27901 tttaatctca gttcccgtta agacaatccc tgcttcaacg gtttcaacaa tgtgataatc
    27961 atgtctagcc tttttatttt gcgccagtat atgaccttca ccttttgcca tagttaactc
    28021 cttttacgac tcttcttttt tgcttgttct ttgtaaaatg gttttttaga ccctttttta
    28081 gttttaccct tggtttttgc cttggctact tctttaggtt cttttttctt tgtcccttta
    28141 gatgattttg aggatttacg gcgatctcgt cgactagctt tatccgacat cttgattttt
    28201 tcaaccacat caaagtcgct aggtaaatac tcaaagtcaa tgtcaccagt ttccttgtca
    28261 gccttaacca atttcacacg aataggttga ccaactttaa agactttacc tgatttttca
    28321 ccttgaaggc ttagcgttct ttcattaaag tgatagtatt ccggtaagct agtgatatga
    28381 accaaacctt caatcgtatt tggcaattct acaaaaaaac caaatttaac gacactggcc
    28441 acgataccgt caaattcttc acccacatac tctgccatat actcagcttt tttcatggct
    28501 tcaaccacgc gttcagcatc aatagcacga cgctctaact gtgaagaaga tgttgctaat
    28561 tctggaatga tctgagtaaa atggtcgcgc ttttcttgac taggttgact gtactcacga
    28621 accatgcggt gaaccaacaa gtcagggtaa cgacgaatcg gactggtaaa gtgagtgtaa
    28681 tattctgcgg ccaaaccgta atgcccatga ttatgttcag aatatcttgc ctgttgcata
    28741 gagcgtaaga gcatcatgtt aagcacttct gctcctggtt gaccttcgac ttttgccata
    28801 aaggcttgca aggcttcctg agaaatcttg ttggctgttc cttgaatgtg aataccaaag
    28861 gtgctagcgt aatcaataaa ttgttgaagt ttttcggcct ttggttcttc gtgaatacgg
    28921 tagataaacg gtaacttagc ttttgcaaaa tgttctgcca cacactcatt ggctgctagc
    28981 ataaaggact caatcatccg ctctgcaatt cccctttgac gaagcaccac atcaaccggc
    29041 attcctttgt cattaacaat aactttagct tcttgcgtat caaaattcaa ggcgcctcgt
    29101 tttgaccgca tagcttccaa aatcctatgc aaagccacca tgagggtcac atcatcagca
    29161 atgctagcga attcttggag cgcttcttca tctcctgcaa tcatgtcatt gaccgtacta
    29221 tacgtcattc ggtaagttgt cttaatcact gattgacaga tttgataatt gaccacatgc
    29281 ccttgagaat taatttccat caaggctgac tgggtcaaac gatcaacgtt agggttcaaa
    29341 gagcagatgc catttgacaa acgctctggt aacatcggaa ccacacgatc ggtgacatag
    29401 acagaagtgc cacgggcgat agcttcctta tcaagggcag atccttctgt cacataatat
    29461 gagacatccg caatatgtac cccaagttca tagttaccgt tatccaatag cttaatatgg
    29521 atagcatcat ctaagtcttt ggcgtccgca ccatcaatag taatggtggt ttcttgacgc
    29581 aaatccacac ggcctattaa atctttagca gttggtgctt ctgaaatagc attggcctca
    29641 gctaaaactt ctgctggaaa ttctgacacg atatccatcg attcaaggac ttctaggaca
    29701 tcaattccaa cgtctccttg atggcctacg atatcacgca cactggctac aaaataatcg
    29761 tgcccgcgtg tcgggtattt atcaatatca accttgatga tttcagtgcc tttaagcaca
    29821 acaggttctt ttttgatata gattttttgt tgaatctttt ggtttttgga cctgatataa
    29881 ccagcatatt ttggtttgtc atcatctaaa ataaaggttc caacagctgt ttttaaagaa
    29941 cggtcaacaa tagcaacgac cttagcttca gccgctgtgc ctttgagacg gtctgctggc
    30001 tttttaacca ctacttcgac agtatcacca tcaatagcat acccaacatc attacgaccg
    30061 ataaacatgt catcttcatt ctcatccaca tgtaagaaac caaagcctgc tttattggca
    30121 cgaaaaacac cctgaacagt aggttctttt ttcttttcac gctcttttct aagagccaga
    30181 ctgccatcat ctgaaaaccg taacaggctc tgactttcca ttttggaaat ggttttaata
    30241 aggctgggaa aatgcttagc tccagccatc tccaaggccg cagccaagtc gttcacattg
    30301 gacttgccat gctccttgag gtaagtcata attctttctt tcatcatacc taatttctat
    30361 ttctgttttg tttgttggag gatatcaatc cgtttcgctg ataaaaaaat gagctagact
    30421 aactctgtca agctcactcc ttatttactt gataaaatgg caagcacaag tgcaatcgct
    30481 agccaaaaaa atactagaac tgctgtgaat cgttgcatga atgcttcaaa gccacgagct
    30541 tttgtacgtt caaacaaggc ttctgaaccg ctgctatcaa aaacattgct acttgggttt
    30601 ttttgaggtt gcataaaaat agcaatcaca agcaataccg acagtactag caatatcgta
    30661 agtagtaagt tgtacatgtt atttcctcca ctattgtcat gaccattcta acacaattct
    30721 tatcaagttt caacatgcag ggtcactttt ctctccttgg ggcagtattt gggaactttt
    30781 attttttccg gatgactgct tttattttta ctggttagat aattgttact gccacactca
    30841 ctacattcta aattaatttt tactctcaca caatatcctt aactttaata tattttcgaa
    30901 atataatcaa actccatatt aaattaacaa acacaatcaa actagtcaca taaaaaacag
    30961 aacggtagcc taacactaca gccacatttg atccgataaa agggccaatg acttgaccaa
    31021 ggttactaaa catttgatta taggcaaata ctctagaaat accttctttt ggtgttaact
    31081 tagttagtaa agagttaata cttggcatca aagcgcccac accaaaccca taagcaaagc
    31141 gtagtactcc taattgaaaa ctggtctgcg ctaaggcact gctaaaatac atgataaagc
    31201 tataacacaa agcggctaac aataaccgat gattgccaaa acgatctcct aattttccta
    31261 agtaagaact gctaaataag ctagaaaacc ccatggccga aactaccagg cctgaagtaa
    31321 acattaagtt gtgagtttga ccaagatgac gaatatacaa tgataaaatg ggggccactg
    31381 attgagctga aatttgaata atcatactag tcacaaacaa accaagcata atctggggac
    31441 tttttacctg ttttaaaatg acttttgtag gtatcatctc aaagcgccta atcggcttaa
    31501 actcttcttt aacataaaca gctgtcatta aagaacataa aaataatatc acaccaacta
    31561 ataagaaaac ttgtcgaatt cctaatagct ctgccaaaat cccccctagt aaaggaccaa
    31621 ttaaagcgcc tgctgtcact cctgttgcta atgtgcctaa agcataacct gactcctctt
    31681 ttggggcttg gctggcaatc aaggcagtgg catttggcac atagcccgca aagacacctg
    31741 ttaataatct caaaatgagt aaccaaaaca cattaggaat aatagctaaa ccacccatgg
    31801 taaaggtcat gacaaaactt gctcgcagca tcataggttt tcggccataa cgatcagcca
    31861 acttccccca aacaggagca aataaagctg aagcaagtgc cgtcacagca accgccagac
    31921 cagcatagta ttctactaat tctttcggag tacctaaatt ttccacatac agagccataa
    31981 aaggcataac aagtgaaaag cttgccccag taaagaagtt accaagccaa gccactttta
    32041 gattttgccg ccagttcact gtcgttaccg ctttatcact aatagatcat catcttcttt
    32101 ccatcgggcc tttgattatt ataacacatt tctgccaaaa acagtgtccc ataatagtgc
    32161 taagtctcat ctgatagcct tcatcgttta cgtaagctac ccagtaaacc tctgaccagt
    32221 tctcgcccaa ttgttctaac cgtcgtacta ataaacgcat ctgttgcttt ttcgacgact
    32281 gtttttcgtg gacgcccaac agaacggcga ctagcttttt tggtagctgc ttgcgccttg
    32341 atggcttctt tagcttcttt ttcgcgtttt gctgtcgcta tcgcttcttg ggccagtcgg
    32401 ttgtcttcta aaaccttagc agctaatttt tcataagcag attcacggtc aatggactga
    32461 ctatatttac tggcaaaagg tgaagactgc acaagctgct gactttctat ctctgacaaa
    32521 acagcaaagc tactcttagg cggcatgata taggcacgtt caacgatgga tggttgtccc
    32581 ttatcattca aaacagaaat caaggcctca ccgacttcta gttctgtaat cacccttgcc
    32641 acatctaagt caggattttg tcggaaagta tccgcagcga cacgaaccgc cttttgttct
    32701 ttcggtgtat aagcgcgcaa ggcatgctga atacgatttc caagctgagc caaaaccgtt
    32761 tctggcaaat caagcgggtt ttgaatcaca aagaaaatcc caactccctt tgaacgaata
    32821 agtcgcacaa tctgttcaac tttttccaaa aagaccttag gagcgtcctt gaacaatagg
    32881 tgagcttcat caaaaaagaa aaccatctta ggtttgtcca aatccccaac ttctggcaat
    32941 aattcgtaca actcagacaa gagccaaagt aagaaggttg tgtaaagggt aggagattga
    33001 aaaagcttag tcgctgacaa aatgttaatg gctccataac ccgaagcaac atctaattgc
    33061 atcaaatcag ccacatctaa agcaggctct ccaaaaaatt ggtgggcccc ttcttgttct
    33121 aatgtcaaga gacttctttg gatagccccg atagattgct tggcgatatt accgtaatgg
    33181 ctggagtagt ctgaggcatg gtcaccaact tcttttaaga tagcctgcaa gtctttcaaa
    33241 tcaatcaaaa gccaaccttt ctcgtcggcg attttaaaga cgatgtttaa gacacctgtc
    33301 tgggtatcat taagattcaa taaacgcgac aacatcatcg gtcccatttc agaaattgtt
    33361 gtccgtaagg gttgaccgtt ttgaccaaaa acatcccaca ttcttacagg aaaggcctga
    33421 ggctggtaat cacttacccc gattgtggct aaacgcgcag ccaatttatc agtcacctct
    33481 cctgctttag taagattaga caggtctcct ttgatatctg ccaaaaagac tggcacacca
    33541 gctaaactta attgctctgc tagcactttt aaggtcacag ttttacctgt tccagtcgct
    33601 ccagcaatca aaccgtgacg atttagcatc cctaattgta aggctatcgg ttgactgcct
    33661 tttgcaattg tcattgtagt cataggcttc ctcttttttg atgatcatac ttgtcgatat
    33721 gtgtttaccc ataagttaaa gtgtttagaa gggtctcact cttaatacta ttataacata
    33781 taacccttga tgcgctaatt taaaagggac cagctagttt tagacagaaa tagcttgatt
    33841 ttgacagcta tgatacgaat ctctcagctt agctacctcg gattggctaa tgacaacaac
    33901 gcagattgta cttgctttat taaggctgca atatccccac tattatcaat cactagactg
    33961 gcatagggct ttttttcctc tattggtaac tgactggcta ttcgctgcct tgcttttcct
    34021 ttgtctaaac gatttctagc cattaagcgc tgcaattgtg tctgagcatc tacataaacc
    34081 agccaaatcg catcaaacca gtcttggtag cccaattcca ttaaaatggg aatatccatg
    34141 aaaaaaatgg cttggctctg tgctaagtgg tcacgcttag ccgccaattc ttccttaatg
    34201 atttgatttt gaatggctga ggatgttgcc atattatctg ggtttgaaaa aagcatttct
    34261 gacagctttg tgcgatctaa ctcaccatct gcttttaaaa tctgattccc aaaagcttct
    34321 ctaagagcct cgtaaagacg accgcctttt tcttgcaagt catggaccac ttgatccgca
    34381 tcaatcacct gataaccagc ttttctaatg accttaacca cagtcgattt tcctgatgca
    34441 atgcctcctg tgatgccaat aatcatggtc tttttttctg gcattttgga caaatgtgcg
    34501 tcccccttcc tccaactttt aatttgacaa tagcctgtcc acaacgagga cacggtttcc
    34561 cagtttgacc ataaacctgc aaatagtcct gcatggtgcc atcagctccc aaagcattac
    34621 ggtaagtacg gacggtcgaa ccgccctttt caattcctaa agctaggatt cgtatggttt
    34681 catcatgcag ccgttttatc tccgccttat tcaacctaga agacgcagtt cctggatgaa
    34741 ccttagcagc ccaaagtacc tcgtccacat aaatattccc taaaccagca accaaggtct
    34801 ggtctaacag atgtggtttt atgggttttt gggaggatag cagagcagcc tcaaaggttt
    34861 ttaacttaaa ggtctctttc tttggttcag ggcctagttt tcgtttggca aaaaacgctg
    34921 ataattgaga ttttgctatc aaatcaaagg ttccaaattt acgcacatct tggtaaacca
    34981 aggtcgaacc atttttcaat tcaaaaaaga catgaaagtg tttgttatca gggacctcat
    35041 ctggaaaaag caaatatttg ccttccatcc gcaaatgtga taccaacact aattgcccca
    35101 aatcaattaa taaatatttt ccccgacggc caactgactg aatgatttga ccaggtaggg
    35161 tcagggcaaa cgtctctaag tcggttttaa ccatctttgg cactttaagc gtaacagcca
    35221 caatttcttg gccaagcact aaagtctcaa gccctcgtcg gaccgtttca acttctggta
    35281 attctggcat aatccgttat ttaaaggaca gctagaccct ttgctcctac aaagaataaa
    35341 ctcagtataa aaaagtcact caatagactc aggtctaggc tattgactgc gtttcaaaaa
    35401 acacagctgt ccttctcctt tcttatttct tattttacga acacatctga tagaaagcaa
    35461 ctcataaatc tgcctcagac cagtcctcat cccccttacc tcccagcact atttggcggt
    35521 aatgttcctt ataactataa tacaatgagt cctctccaag atacttaaaa atctgcaagg
    35581 cctgccgcat gtcctcttct ccagacattt cttcctgctt taatttataa tacccatgca
    35641 catataagaa aacagtcttt tcataaaaat tgagctcgtc tcgcagcaaa agatcaattt
    35701 tgttaatcaa atagcgacta tgttcaaagc aagaatggtc aatactgata atgagacaat
    35761 taatggataa ctgagtcact aacattttat tggttttatt ttgttccgaa taagcaaaag
    35821 atgctaccat ttccttagtc aataaaaata gggtattgta attcatgaag cgcgagcaat
    35881 tccccagaag aatgatttca taatatcccc actgttccac tttaaataaa taatctgtga
    35941 ggcgagtcaa ttcttcttga ctaggtgcaa ttgaggagtc aatagaaaaa agaatagctt
    36001 taatcatcgt tcgttcataa tctttatggg cataatcctt caacagtttg gtcaatttaa
    36061 ccacgttttt ctcagcataa cattttctcg cctgacttaa gagcgtgaaa aaatgcgtat
    36121 gcgtcttgga atgagccgaa acaaattcgt caatcgtaat attcagttta tccaaaagat
    36181 taagcaagcg actgcaggtg atttctgatt cccctcgttc aaatcttgaa atttgtgact
    36241 tggacaggta ttcatctgct aaaaaagaaa tgctaacctg cttaccctta cgtaatcgcc
    36301 ttaaggtttt tccgagttct ttttccattt ttcccacttt cacaacaaaa aattaaaatc
    36361 tgctaaatcc attatataat acaagtacaa aatagaaaat cagaaaggag acagtcatga
    36421 aaaaagttaa taaagctttg ctttttacat tgatcatgga tatcctgatt atcgttggtg
    36481 gataaccttt cgcttaaaaa caatccactt ttgggaaacc cttatattta gtttgtaaca
    36541 ggaaaactaa taaatattaa aaagagtgtt agcgacctag cggcagcttt actcttaagt
    36601 gcgtttgctg ccattgacag caaggtcaaa gctaatgaaa cagcaggagc ataccgttat
    36661 gatgtgtctg aacaaaacac ttactatcaa actatcagct ccattaacca gcaacgacct
    36721 ggtgccactc tcgacgaatg aggtgattat tatggaaccc ttacttcatt tacttcccaa
    36781 aatcgtacca gtacttatac acttggtaga caaagccaac gtatcatggt taacagtgat
    36841 attacagtta cctatactct taaagacaat ttggtagccc ttcataagga atataaaacc
    36901 tttgtcaaaa aaatattaca gataaaccga tcaccagtga ctacgacaag gctaaggtta
    36961 tttataacca tttactcaac gaatacactt acgccacaaa ataacttagc accaatcaca
    37021 aaaccgtaag cagcatcaac aatcatactc ctaaagctct ttatcgtggc aagtgtggtg
    37081 tttgtcaagc ttatgccgtc atgtttaaag atatggcaga agctgcaggc ttatcagctt
    37141 ggtatgtgat aaaaaaggat tgaatgagca ccatgcttga aacatcgtta ccattcatgg
    37201 tgttaattcc tacgttgatg ctacctggaa tgattcagca tacaccacaa ggtatttcct
    37261 tgctggaaaa gacgtgatga atactgagta ccgtttagat agccagtaca accacttggc
    37321 taatactatt ttaactacca gctaccaaaa aggctaaact aacaacaact acatttttca
    37381 actagaaagg cctaaacaat gaccaaaaaa cagttttatc ttatcttagc tattagcata
    37441 ctgtgtttta ttcttgccgt cagccctaaa ccaccaattc ctaaacacac tattgaaagg
    37501 aaaaggactg cgatgaccat aggccactca aaacaaaccc ctcacaaaaa agaagccctt
    37561 aaacacaaaa aaaacaaaaa taaggctcta tttcttccaa gtggggacag cgctatcaaa
    37621 tctgttatta gcatgacagt tagactcctg ttagatgacc aatattagct ctcctaaaat
    37681 cagtggacac taccatttcg gtagtgttct tttttaagct aatctcatca ccacgctttc
    37741 ttcaagctaa aaaactgatc atgaaagagc aaccccatca ccctaacagc ccactttgcc
    37801 cgcactttct taaaaagtta ggagaaaacg atataccaat tattttctga tcccagctcc
    37861 ctatcattaa caagatgtgt agcacggacc tacatacagg taagctagag tcattctcgc
    37921 tacttcggac aaaaagaggc cggtagttcc ctgacctctt ctttatttat cttgtaacac
    37981 tcctcttctt agcgagactt actagcacat aattcttaag cactcttatc atttagacta
    38041 ggaataacac catctgaatg aagcaaatga tgcgtcagat ctgccagagt atcatctcca
    38101 taagtaatcc ctgtttggtg ctgtttctga tgaaaatcag atgactcctt aactttagca
    38161 gtcacctgct ggtcacttgg atgaaaatta atttctctct tggcttcagc cgcaactggc
    38221 tcgaggaaca acttattgac attacggtct tcctccaaat gattaaggat agcagcatca
    38281 tcttctaacg aaagaacctt cttgggtaac aaaaaggggt tatttagcct tggctcagta
    38341 acgacttcct tggctactcg tgatagttta ttattgtctt cctcaggtat caaacggtta
    38401 ggcaatcgat gcaattcttc cggctatgag gcgctcacag ctacttcaga agaaacctcc
    38461 aagccctttc ctgtttgcct gtctaactaa agcaaccggc aaattattaa ctaacttttt
    38521 aacctgtcct cagcgctcat ttcaacacta ggaagagctg tcgctggttc tagccagcta
    38581 ccaacttctg ctggtcttac cttaacatcg ctggcaggct tctccttctc atcttcatta
    38641 ggtctaaaag gctcaaaccg ccctgttgat ttttcatcta tgacctttat attgtctttt
    38701 actacatgag acagtggttc gacatttacc atagcttcat gctcgttaag gctctggtct
    38761 cgtttgaatt taccaggaat accaggagtc ggtgttccga cagattgatc ggcttcagta
    38821 ggctctttta cggagccttt ttcccataac accgctgatg gaatgatatc cgtagagcca
    38881 tctccgtaaa taatcgtggc ctgaccacct cttggattaa tagatacctt aatattgtct
    38941 gggaaagccc cactacttcc tgtggttaaa agggcacccg catttttaat gagagttgtc
    39001 attcgttcaa taccagcaac gccaatatgg ttcaagtcat ctactggtaa ctgatcaaca
    39061 gatttcatgg tgtatttcca agcttgcggt tgaacaagaa aaacagttcc tttatacatt
    39121 gtgttatgca tatcatcaat gactttgaaa taccttgtat atttggtttg tacattaggg
    39181 ttagcaacgg gaacagtgct ttcaggaact cctgacatcg taaaaatccc tctagcagaa
    39241 gtttgaaagc cctcataaac atgacccata ccaggaacag tctgagcggt caaagactta
    39301 ccagaaccat ccaaagcaat gcgaatatct ttcatcagat acatctgatt ggtgtaggta
    39361 aacctaaccg tgttagcctc acctcgatac aaaaccactt gggagggctg atccgaagaa
    39421 aactgagagg ttgtttcttc cttatagcca actccaaaac gtctatcaga cgaatggact
    39481 gaatctgccg caactgtact actcaaactg actagtaaag tagccgcaca accaactaat
    39541 aaactgctca ttttaacttt aacatctctt aacatcatca tgttcctttc attatttttt
    39601 atttaggaat tcgtcatact atattttctc atattagtct aacaaaaaac ccgtaattct
    39661 atataaatca tataaaatga cgagctttgg caaagatgag tatagcagga tggaaaacag
    39721 attgtttctg aaacttcaaa ggtatcttct tgtcacgaac tattctttaa ttgaatcgg
//
LOCUS       DATUYR010000013        33782 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.013, whole genome shotgun sequence.
ACCESSION   DATUYR010000013 DATUYR010000000
VERSION     DATUYR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33782
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.013"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            32..1093
                     /locus_tag="VCI33_000583"
     CDS             32..1093
                     /locus_tag="VCI33_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831525.1"
                     /translation="MTFFLMPILTTLLALSLSFSNNNQAKIGILDKDNSQISKQFIAQ
                     LKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSDKVLQGKSQKLNIRSIANSE
                     ITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQLNYDVKQVTLTDRSRSKAV
                     SSTTTGFLLILMLGSTSVIYSGILADKSGQLYHRLMLSNLSRFRYMLSYVCVGFVAFT
                     IQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFGLLIGAITQNSQQSSQLANL
                     IVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIAIFQSGGTLSQAWPYLLALM
                     GTALALISFSSLLLKPTKL"
     gene            1665..4397
                     /locus_tag="VCI33_000584"
     CDS             1665..4397
                     /locus_tag="VCI33_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEP2831526.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTSLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVIKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPCLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVNRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      4562..4677
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            4675..5175
                     /locus_tag="VCI33_000585"
     CDS             4675..5175
                     /locus_tag="VCI33_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEP2831527.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            5369..7327
                     /gene="ligA"
                     /locus_tag="VCI33_000586"
     CDS             5369..7327
                     /gene="ligA"
                     /locus_tag="VCI33_000586"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEP2831528.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWNFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKEDFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            7341..8363
                     /locus_tag="VCI33_000587"
     CDS             7341..8363
                     /locus_tag="VCI33_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP2831529.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            8755..8952
                     /locus_tag="VCI33_000588"
     CDS             8755..8952
                     /locus_tag="VCI33_000588"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEP2831530.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            8987..9703
                     /gene="atpB"
                     /locus_tag="VCI33_000589"
     CDS             8987..9703
                     /gene="atpB"
                     /locus_tag="VCI33_000589"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEP2831531.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            9721..10215
                     /gene="atpF"
                     /locus_tag="VCI33_000590"
     CDS             9721..10215
                     /gene="atpF"
                     /locus_tag="VCI33_000590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEP2831532.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            10215..10751
                     /locus_tag="VCI33_000591"
     CDS             10215..10751
                     /locus_tag="VCI33_000591"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEP2831533.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQTVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            10767..12275
                     /locus_tag="VCI33_000592"
     CDS             10767..12275
                     /locus_tag="VCI33_000592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEP2831534.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            12291..13166
                     /locus_tag="VCI33_000593"
     CDS             12291..13166
                     /locus_tag="VCI33_000593"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEP2831535.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            13328..14734
                     /gene="atpD"
                     /locus_tag="VCI33_000594"
     CDS             13328..14734
                     /gene="atpD"
                     /locus_tag="VCI33_000594"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEP2831536.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDDLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            14747..15163
                     /locus_tag="VCI33_000595"
     CDS             14747..15163
                     /locus_tag="VCI33_000595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEP2831537.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            15429..15686
                     /locus_tag="VCI33_000596"
     CDS             15429..15686
                     /locus_tag="VCI33_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEP2831538.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            15751..17022
                     /gene="murA"
                     /locus_tag="VCI33_000597"
     CDS             15751..17022
                     /gene="murA"
                     /locus_tag="VCI33_000597"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP2831539.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTSLHGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            17026..17214
                     /locus_tag="VCI33_000598"
     CDS             17026..17214
                     /locus_tag="VCI33_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP2831540.1"
                     /translation="MTNGLKYVLEQMLLLLVIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            17250..18114
                     /locus_tag="VCI33_000599"
                     /pseudo
     CDS             17250..18114
                     /locus_tag="VCI33_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            18397..19440
                     /gene="pheS"
                     /locus_tag="VCI33_000600"
     CDS             18397..19440
                     /gene="pheS"
                     /locus_tag="VCI33_000600"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEP2831541.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            19650..22055
                     /locus_tag="VCI33_000601"
     CDS             19650..22055
                     /locus_tag="VCI33_000601"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEP2831542.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSATSIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEVAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNELNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLASMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALPSDQTFAEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            22165..22542
                     /locus_tag="VCI33_000602"
     CDS             22165..22542
                     /locus_tag="VCI33_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEP2831543.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVMVTITLEGLSHV"
     gene            22535..22921
                     /locus_tag="VCI33_000603"
     CDS             22535..22921
                     /locus_tag="VCI33_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEP2831544.1"
                     /translation="MSDAGLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            22994..24070
                     /locus_tag="VCI33_000604"
     CDS             22994..24070
                     /locus_tag="VCI33_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831545.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSILLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GISNKTITTAVLMQTFFLSFLGSGLGLLGTWLTSLLLPTVVPFQSNWFLYLAIFVSMI
                     CFALLGTLFSVFNIIRIDPLKAIG"
     gene            24080..24748
                     /locus_tag="VCI33_000605"
     CDS             24080..24748
                     /locus_tag="VCI33_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831546.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGITGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYRMQDGEL
                     RQER"
     gene            complement(24853..25770)
                     /locus_tag="VCI33_000606"
     CDS             complement(24853..25770)
                     /locus_tag="VCI33_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEP2831547.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSSVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRTVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            25921..29136
                     /gene="rexB"
                     /locus_tag="VCI33_000607"
     CDS             25921..29136
                     /gene="rexB"
                     /locus_tag="VCI33_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEP2831548.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVIVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFFKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELISFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMAQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            29097..32765
                     /gene="addA"
                     /locus_tag="VCI33_000608"
     CDS             29097..32765
                     /gene="addA"
                     /locus_tag="VCI33_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEP2831549.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLGQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDITHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYSIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNIFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QTPQLLNPDDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKVAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKVKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALREIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            32905..33717
                     /locus_tag="VCI33_000609"
     CDS             32905..33717
                     /locus_tag="VCI33_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP2831550.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
BASE COUNT        10621 a         5848 c         6977 g        10336 t
ORIGIN      
        1 tgttaagata aagcgcaaaa aaacaagtta tgtgacattc tttttaatgc ctattttaac
       61 gacacttttg gctttatctt tgagtttttc aaataataat caggctaaaa ttgggatatt
      121 agataaagat aatagtcaga tatctaaaca atttattgct caactaaagc aaaacaaaaa
      181 gtacgacatt tttaccaaaa tcaaaaaaga gcatattgat cactaccttc aagataaatc
      241 tctagaagcg gtattgacta ttgacaaagg attctcagat aaagtgttgc aaggaaaatc
      301 tcaaaaactc aatatacgtt cgattgctaa tagcgaaatt acagagtggg taaaggcaca
      361 gaccaattat ctattggaaa actataatat tataggggat gtggctctag gtaacgaaga
      421 tacttttaat agaattctcc aaaaaaacca acagttaaat tatgatgtta agcaagtaac
      481 tttgacagac cgatcacgta gcaaagcagt atcttccaca acgacaggat ttttgcttat
      541 tctaatgtta ggtagtacga gcgtgattta cagtggtatt ttggcagata aatctggtca
      601 gttataccat cgattaatgt tatcgaactt atctcgtttt cgctatatgt tgagttacgt
      661 ctgtgtagga tttgttgctt ttacgattca aattgtcatt atgctgagtt tgttaaaagt
      721 atttaatatt agtttttttg ttccgacatc acttcttttg attatttttt tcctttttag
      781 tttattagcg ataggctttg gcctgctgat tggagctatc acacaaaatt cccaacaaag
      841 tagccagtta gctaatctta ttgtaatgcc gacttcaatg ttggctggct gtttatggcc
      901 attgtctatt acgccttctt atatgcaagc aattggtaag ttattaccac aaaattgggt
      961 tttatcagcc atagctattt ttcaaagtgg agggacctta tctcaggctt ggccatactt
     1021 gttagctcta atgggaacag cactagcttt gattagtttt tcaagtttat tattaaaacc
     1081 taccaaacta taattagtct tttgttaaaa aatactttcc taattagcta actaccaggt
     1141 agccctttta ttggcttata tgcaataaaa gggttgcttt ttgcttatat tttctctagt
     1201 gcacttatct tttgttaaaa gcgattgcga tgttactgtt gttactgtgt tttttagaga
     1261 gcatattatc aaaagcgacc tctctagaaa gttcgttttt gttgacagat tagttgtcaa
     1321 agtattttaa aaaaacgttt tcaataaaca taaaaactaa tgttattaaa aaaagttgtg
     1381 aaaacgtatt tattttatgg aatgtttttg ttacaataag catgtatcga atgctataaa
     1441 gctgtcttgt tcgctatcag ggatgcttgt gatgttatgt cagatcaaac aaatctttaa
     1501 ttgggtgact taacagatta aaagaaaaga tctaatcgtg ttacttgata gtataattaa
     1561 tgcaaacaga aagtgtatcc caattcatag tctcaggtgg aggttaatat tcaaaaataa
     1621 aatcttgttg ttgtcttaaa ctaatcacat taaaaggaga tatcatgatt aacaagaaat
     1681 atataatacc tgtttcattg ttgacactag ctattacgct tactagtgtt gaagaagtta
     1741 cttcacgcca aaatttgact tatgccaatg aaatcgtaac acaaaggcca aagagagaat
     1801 ctgttattag tgataaatcg aattttcccg tcatatcacc ttacctagca agtgtggatt
     1861 ttggtgagag aaaaacatct ttgccaacac ctgataaagg agtaaaagta actactgaac
     1921 agtctattgc tcaagtaaga aaggggcctg aagaaagaac ctatactgtt actggcaaga
     1981 ttacgagtgt gatcaatggc tggggaggct atggctttta tattcaagat agtgaaggta
     2041 ttggacttta tgtttatcct caaaaagatt taggatacag taagggagat attgttcaat
     2101 taacaggtac acttactcgc tttaaaggtg atttacaact ccaacaggtg actgcacaca
     2161 aaaagttaga gttatctttt ccgacttctg ttaaagaagc agtaatatca gaattagaaa
     2221 caacaacacc ctcaacatta gttaagttat ctcacgtgac agttggagaa ttatcaactg
     2281 atcaatataa caacacatct ttccttgtaa gggatgatag tggtaaaagt atagttgttc
     2341 atatagatca tcgtacaggg gttaaagggg ctgatgttgt tactaaaata agtcagggtg
     2401 atttgattaa cctcacagcc atattgtcta ttgttgatgg tcagttacaa ttaagaccgt
     2461 tttctcttga acaattggaa gtggttaaaa aggtcacaag ctcaaatagt gatgcttcat
     2521 ctcgtaatat tgtgaaaata ggcgagattc aaggagctag tcatacgtcg ccacttctca
     2581 aaaaagcggt caccgtagaa caggttgttg tcacttattt agacgattcc actcattttt
     2641 atgttcaaga tcttaatggt gatggtgatt tagcgacttc agatggtatt cgtgtttttg
     2701 ctaaaaacgc taaggttcaa gtcggcgatg ttttgaccat ttcaggtgaa gtggaagaat
     2761 tctttggtcg tggttatgag gaacgtaagc agactgacct taccatcacc caaattgtgg
     2821 ctaaagcagt gatcaaaaca gggacagctc aagttccatc accgcttgtt ttagggaaag
     2881 atcgtatcgc gccagccaat attattgata atgatggctt gcgtgtgttt gatccagaag
     2941 aagacgctat tgattattgg gaatcaatgg aaggcatgtt agtggcggtt gatgatgcta
     3001 aaatccttgg tccaatgaaa aataaagaaa tttatgtctt acctggctct agtacaagac
     3061 cgttaaataa ttcaggtgga gtattacttc cagctaattc ttataacaca gatgtgattc
     3121 ctgttctttt caaaaaaggc aaacaaatta ttaaagcagg agactcttac aaaggaagat
     3181 tagctgggcc agtatcttat agctatggta attacaaggt ctttgttgat gacagcaaaa
     3241 acatgccatg tttaatggat ggtcatctaa aacctgaaaa aacaaacttg caaaaagacc
     3301 ttagcaagtt aagcattgct tcttacaata ttgaaaactt ctcagccaat ccttcttcaa
     3361 ctaaagatga gaaggtcaaa cggattgccg aatcctttat tcatgatctg aatgctccag
     3421 acattattgg attaattgaa gtccaagata ataatgggcc gactgatgat gggacaacgg
     3481 atgcgacaca aagcgcgcaa cgcctcattg atgctattaa aaaactaggt ggcccaactt
     3541 atcgttatgt tgatattgct ccagaaaata atgttgacgg aggtcaacca ggtggtaata
     3601 ttcgaacagg attcctttat caaccagagc gcgtcagcct ttctgataag ccaaaaggcg
     3661 gtgctcgtga tgctctaact tgggttaatg gagaattaaa ccttagtgtt ggtcgaattg
     3721 atccaactaa cgccgcttgg aaagatgttc gtaaatcgct agcagcagaa tttatcttcc
     3781 aaggtcgtaa agtcgttgtt gttgcaaatc atttgaactc taagcgtggg gataatgctc
     3841 tttatggtcg tgtgcaacca gtcactttta aatctgagca aagacgtcac gtcttggcta
     3901 atatgctagc acaatttgcg aaagaagggg caaaacacca agctaatatt gtgatgctag
     3961 gtgactttaa tgattttgaa ttcacaaaga cgattcaatt aatcgaagaa ggtgatatgg
     4021 ttaacttggt gaaccgacat gatatttcag atcggtattc ttattttcac caaggcaata
     4081 atcagaccct tgataatata ttagtttcac gccatttact tgatcactac gaatttgaca
     4141 tggttcatgt gaattcccca tttatggaag ctcacggacg cgcatcagat catgatccat
     4201 tgttacttca attatcattt tctaaagaaa atgataaggc agagtcttct aaacaaagtg
     4261 taaaagctaa aaaaacttca aaaggaaaac tgttgccaaa aacaggagat agtcttgttt
     4321 atgtgataac gctactagga acggctagtt tattagtgcc tattttatta ttgactaaag
     4381 gcaaaaagga atcatagcga tttgaagaat tggagttcat aaagagtcgt taaaggcttg
     4441 atatacttac gttagacctt aagctaaaat ggatgactat ataaaaacta atattgataa
     4501 caaaagaaga taacaataag acagctttac ttgctaaaga cttttgtcaa gctaattgtt
     4561 ttatggtaca atgtaaccgt tgctttgcaa cccttggtgc ttagttccct ttcaccaagc
     4621 atattagaaa cgtagatagc ttttgagagt tatccagacg ttaaaggaga aaaaatgaca
     4681 aaattgactg tacacgacta tgttcacatt gggctagtag ctgccttgta tgttgtctta
     4741 actattaccc cgcctttaaa tgcgatttct tatgggatgt atcaatttag aatatctgaa
     4801 atgatgaatt tcttagcttt ttatcatcgc aagtatatta ttgcggtgac tcttggatgt
     4861 atgattgcta acttttatag tttcggactg attgatgtct ttgtaggcgg cggttcaact
     4921 cttatttttg tgaccttagg tgttattttg tttagtaagt atcaaaaaga ttatcttttt
     4981 aatggcattt ttaataaagc ttttgtttac ttttctttct tttttgcaac ttccatgttt
     5041 actgtagcaa tcgaacttta cttctttgga gctccatttt tattaacatg gtttacaact
     5101 gcgttgggag aattagtatc tctgctcata ggatcactta ttattgataa gttgtctcaa
     5161 agaatttcct tttagtaatg catcatactc aaaagagttg ggtcaatacc cttctctttt
     5221 ttatgttgtt tgtaatgtta aaatcgttct agatagaaat atttaacact ttagctaata
     5281 gtaccatatg gagtggccaa cgattacttt tgatgctgtt atgatataga aatagctcat
     5341 ttgttatctt ttatgataaa ataaaagtat gaaaaaacgc ataaaagaat taacagatct
     5401 tcttaaccgt taccgttatg actattatac taaagatgcc ccatcagttt ctgacagtga
     5461 ctatgataag ctttatcgtg aactggtgac tttagagcag tcatatcccg agtatgtctt
     5521 gcaagatagc ccaacgcagc aggttggagg gactatttta aaggggtttg aaaaatatcg
     5581 acatcagtat ccgcttttta gtttgcagga tgctttttca cgtgaggagc tggatgcttt
     5641 tgataagcgc gtcaaggccg aatttccgaa tgccacctat ctagcagagt taaaaattga
     5701 tggtttatct atttccttga gttatgagaa tggcttctta caggttggag caacacgggg
     5761 tgatggcaat atcggtgaaa atatcacaga aaatattaaa aaaataaagg atattcctca
     5821 ccaattgagt gagcctttaa ccatcaccgt tcgaggagag gcttacatgt ctcgtcagtc
     5881 ctttaaagcg atcaatgaag cacgccaaga aaatggggag actgaatttg ctaatcctag
     5941 aaatgctgcc gctgggacac taaggcagct agatacggca gtggttgcta agcgtcaact
     6001 agccactttt ttgtatcaag aagctagtcc gacagctaga aatcaacaaa atgaggtctt
     6061 agcagaatta gcagatctag gtttttcagt aaatccgtat tatcaactca catcgtctat
     6121 ggatgagatt tggaatttta tcaaaactat tgaggcaaaa agagatcaat tagcttatga
     6181 catagatggc gttgttatta aagttaatag cctagccatg caagaagagc tagggtttac
     6241 ggttaaagct ccacgctggg ctattgctta caaatttcca gcagaagaaa aagaggcaga
     6301 aatcctttcg gttgactgga cagtgggacg aacaggcgtg gtcacaccta cagcgaatct
     6361 aactcctgtg caactagcgg ggacaaccgt gagtcgtgca accctacata atgtggatta
     6421 tattgccgaa aaagatatcc gtattggcga tacagtgatt gtatataagg caggggacat
     6481 tattcctgct gtcttaaatg tcgttatgag taagcggaac cagcaagaag tcatgcttat
     6541 tcctaaacta tgcccatctt gtggtagtga attagttcat tttgaagatg aggttgcttt
     6601 gcgttgtatt aatccgttgt gtcctagttt gattcagcga agtttggagc attttgcaag
     6661 tcgtgatgcc atgaacatta ctgggctggg accagctatt gtggaaaaac tatttttagc
     6721 ggggtttgtt catgatgttg ctgatattta ccagttgacc aaagaagatt tcatgcaatt
     6781 agatggcatc aaagaaaagt cagctgataa attgctcgct gcgattgagg cttctaaatc
     6841 aaattcagct gaaaaactgt tatttggtct aggaattcgt catattggct ctaaggttag
     6901 tcgcttaatt ttagaggtat acggagatat ttctgctctt ttaaccgcta aagaagagga
     6961 aatcgcaagg atagatggtc ttggctcaac gattgctcaa tcacttactc aatactttga
     7021 acaaaaaacg gctgcgattt tagtagacga gttaaagaca gcaggtgtca acatgcatta
     7081 ctcaggtcaa aaagttaata gtgacgcagc gctttttggt ctaactgttg ttttaactgg
     7141 caaattaaat cagctgaacc gaaatgaggc caaagataaa ttagaggcat taggtgccaa
     7201 agttactgga agtgtatcta agaaaacaga tttagttatt gcaggtagtg acgctggttc
     7261 taagctagaa aaagcaaaga gcttaggaat tcggattgaa gatgaagatt ggctacgcca
     7321 gctttaagtg aggtaatgtt atgaaaaaac aattgagagc acgtctcatt tataatccaa
     7381 cgtcaggaca agaacttatg agaaaaagtg ttcctgaagt tttagacata ttagaagggt
     7441 tcgggtatga aacatctgcc tttcaaacaa cagcccaaaa gaactctgct ctaaatgaag
     7501 caagaagagc ggcaaaggca ggttttgact tattgattgc tgctggaggt gatggcacta
     7561 ttaacgaagt ggtcaatgga attgctccct tgaaaaagcg ccctaaaatg gccattattc
     7621 caacaggtac cactaatgat tttgctagag cgttaaaagt accaagagga aatccgagcc
     7681 aagcagcaaa acttatcgga aaaaatcaga ctatccagat ggatattggt agagctaaga
     7741 aagatactta tttcatcaat attgctgcag caggaagttt gacggaatta acttacagtg
     7801 taccaagtca gttaaaaacg atgtttggat acttggctta tcttgctaaa ggagtagaac
     7861 tgctcccaag agttagcaat gtgcctgtta aaattacgca tgacaaaggc gtctttgaag
     7921 gtcaagtttc tatgattttt gcagctatta ccaattctgt tgggggattt gaaatgattg
     7981 ctcctgatgc aaaactagat gacggtatgt ttacgctcat tttaataaag acagcaaatc
     8041 tttttgaaat tgtacatctt ttgcgtttga ttttggatgg tggtaagcat attacagatc
     8101 gtcgtgtcga atacattaag accagtaaaa tagtcattga accccaatgt ggtaagcgaa
     8161 tgatgattaa tttagatggc gagtacggag gagatgctcc aattacatta gagaacctaa
     8221 aaaatcatat cacttttttt gcagatactg atttgatttc tgatgacgct ttagtcttag
     8281 atcaggacga attggaaatt gaggaaatcg taaagaaatt tgctcatgaa gtagaagact
     8341 tagaacaaga attagaagaa taatacaata gttattagct actgacagtt tagtcaaatg
     8401 cactactatt agaatctaag atttttaaat taattgaggt agtgtttttt gatgtgctca
     8461 aagagtcaat ttattcatat gaggggccca tttgaggtct ctcttttttg atcatatgca
     8521 agaaaatgcc atgttttagg gatgttatta tacccttttt ggaatcttgt tgcctttttg
     8581 ttataataag agtgtaattc tatttttaaa aagatcttaa caaagacaga ctcgtttatc
     8641 aaaaaggtta gtattagtct aagaaagcgt actcagtcta aagaaatctg ttgacattct
     8701 taattttaag gaatagaata gcaaagtaaa aaaaatttaa aggagtattc tactatgaat
     8761 cctattttcg cattggcttt ggcctgtttt ggtgtttctc ttgccgaagg tttcttgatg
     8821 gcaaatcttt ttaaagcagc atctcgccaa ccagaaatca ttggtcaatt acgttcatta
     8881 atgatcttgg gtgttgcctt tatcgaaggt actttctttg ttacccttgt tatggccttc
     8941 attcttaaat aacagcctat ttgataatag aaaaggaggg tgttagatgg aagaagctaa
     9001 aatacccatg ctaaagcttg gtccaataac ctttaatttg accctacttg ctgtttgtat
     9061 tgtcacaatt gctgttatct ttgcctttgt tttttgggca agtcgccaaa tgaaattgaa
     9121 accagaaggg aagcaaactg ctttagaata tttaatcagt tttgtggatg gtattggaga
     9181 agagcactta gatcataatc tacaaaaatc ttattcgctg ttactcttta ccatttttct
     9241 ctttgtggct gtcgctaata atttagggtt atttactaag ttagagacag ttaatggcta
     9301 taacctatgg acctcgccaa cagccaatct ggcttttgac cttgctctat ctctttttat
     9361 taccttaatg gtacacattg aaggggttag acgtcgtggc ttggttgccc atttgaaacg
     9421 tttggctaca ccgtggccaa tgactccgat gaatttatta gaagagttca caaatttctt
     9481 atcacttgcc attaggttat tcggtaatat ctttgccggg gaagttgtta caggtttgat
     9541 tgttcaactg gccaattatc gagtttattg gtggccgatc gctttcctag tcaatatggc
     9601 gtggacagcc ttttcagtct ttatttcctg catacaggct tttgttttca caaaactgac
     9661 agcgacctat ctaggaaaga aagttaatga atcagaagaa taaggaagga gaacaaacga
     9721 atgtcaatca catttggtga actcgttggg aattttattc tagtaacagg ttctgtaatc
     9781 gtcttgttac tcttgatcaa aaaattcgct tggggtgcaa ttgaatcgat tttacagaca
     9841 cgctcacaac aaatctctcg agatattgat caggctgagc aatcacgttt aagtgctcaa
     9901 cagttagagg caaaaagtca agctaaccta gatgctagtc gttcacaagc aagtaaaatc
     9961 attagtgatg ccaaagaaat tggtcaatta caaggtgata aattggtggc agaagctact
    10021 gatgaagcaa aacgcttgaa agaaaaagcg ttgacagata ttgaacaaag caaatcagac
    10081 gctatttcag cagtcaaaac agaaatgtct gatttaacgg ttcttttagc ggaaaaaatt
    10141 atgggagcca atcttgataa gacggcgcaa agccagctta ttgacagtta tcttgatgac
    10201 ttaggagaag cttaatgacc aaaaaagaac aggctcttat cgagcaatat gcaaaaagcc
    10261 ttgttgaggt agcaagtgaa catcattctc ttgatgcctt acaagctgat gtgcttgcga
    10321 ttttagagac gtttgtaaca actaatcttg atcaaagtct atcaagtcta gctgttccgc
    10381 atgctgaaaa aataaaattg ctaaccttat taaagggaaa taattctgtt tatatgaata
    10441 atttcttgaa tctcatttta caaaacgaac gtgaagctta tttgtatcaa atgcttcaga
    10501 ctgttttaaa tgaaattgca atagtttcaa atcagtatga tgtgactgtt acctcatcac
    10561 tacctttgac tgaggagcaa aaaagccgtg ttcgagcagt tgttgcaaag aaatttgctg
    10621 taacagcggg tcgtttaatt gaaaaagtag atccttcgct tattggagga tttattatca
    10681 gcgtcaataa taaagtaatt gataccagta ttcgtcgtca attgcaagca tttaaaatga
    10741 atttaaaata gaaagtggtg tgacttttgg caattaatgc acaagaaatt agcgctttaa
    10801 ttaaaaagca aattgagaac ttccagccaa atttcgatgt cacagaaact gggatcgtta
    10861 cctatattgg tgacggtatt gcacgtgccc gtggattaga taatgccatg agtggtgaac
    10921 ttcttgaatt tgaaaatggt gcctatggga tggctcaaaa ccttgagtca aatgatgtag
    10981 gtattattat tcttggtgat ttttcagcaa tccgtgaagg agatgtggtt aagcgcaccg
    11041 gtaaaatcat ggaagtacca gttggtgaag ctctcattgg acgtgtggta aacccacttg
    11101 gacaaccagt tgatggcttg ggagatattg aaacaactgg ctttagacca gtcgaaacgc
    11161 cagctcctgg tgttatgcag cgtaaatccg tttctgaacc cttacaaact ggattaaaag
    11221 caattgatgc tttggttcca attggtcgtg ggcaacgtga gttgattatc ggagaccgtc
    11281 aaacaggaaa aacatccgta gctattgatg cgattttgaa ccaaaaaggt caagacatga
    11341 tttgtatcta tgtggctatt ggtcaaaaag aatcaaccgt tcgtacacaa gttgaaaccc
    11401 ttcgccgtta cggtgccttg gattatacta ttgttgtgac agcgtcagcc tcacaaccat
    11461 caccattgct cttcatcgct ccttatgcgg gtgttgccat ggctgaagaa tttatgtacc
    11521 aaggcaagca tgtcttgatt gtctatgatg atttatcaaa acaagcggtg gcttatcgtg
    11581 aattgtctct cttgcttcgt cgtccaccag gtcgtgaagc ttacccaggg gatgttttct
    11641 acttgcatag ccgtcttttg gaacgctctg caaaagtttc tgatgactta ggtggaggtt
    11701 ctatcacagc tctaccattt atcgaaacac aggccggaga tatctctgca tatattgcga
    11761 ctaacgttat ttcgattaca gacggacaaa tcttcttgca agagaacctc tttaattcag
    11821 gaattcgtcc agcgattgat gcaggatcat ctgtctcacg tgtaggtggt tcagctcaaa
    11881 ttaaagcgat gaaaaaggtt gctggtactc ttcgtttgga tttggcttcc tatcgtgaat
    11941 tagaagcctt tacccaattt ggttcggacc ttgatgctgc aacacaagca aaacttaatc
    12001 gtggtcgccg tacggttgaa atcttgaagc aaccattgca taaaccgtta cctgttgaaa
    12061 agcaagtcgt tattctttat gcccttactc atggtttctt agatgatgtt cctgtagatg
    12121 atattttggc ttttgaagaa gctctttacg actactttga cgttcattac aatgatttat
    12181 ttgaaactat ccgtaccaca aaagaccttc ctgaagaagc tgcccttgat gccgctatta
    12241 aagcgtttaa agaacactca aactttaagt catagaggga ggatactagt atggcaggct
    12301 ctctaagtga aattaaagct aaaattattt caactgagaa aacaagtaaa atcactagtg
    12361 ccatgcgtat ggtttcttct gcaaaacttg ttaaatctga gcaagcagct cgtgattttc
    12421 aaatttacgc ctcaaaaatt cgccagatta ctacagattt attgaaatca gaattaacca
    12481 taggatctga taatccgatg ttggtgtctc gtcctgtcaa aaaaacaggc tatattgtca
    12541 tcacgtctga taaaggactt gttggaggtt acaattcaaa aatcttgaaa tctgttatgg
    12601 atatgattac agagtaccat gctgatggtg actatgaaat catttctatt ggtagtgttg
    12661 gttcagactt cttcaaagca agagggatga atgtggcttt tgaacttcgt ggacttgcgg
    12721 atcaaccaag ttttgaacaa gtgagacaaa tcatttcaca atccgttgac atgtttgtca
    12781 atgagatttt tgatgagtta tatgtctgtt acaatcacca tgtgaatagc ttaaccagcc
    12841 aagtccgtgt tcaacagatg ttgccaattt ctgacttggt tgctgatgaa gcagcagaag
    12901 aaggtgttac tgggtttgaa ttggaaccta atcgtcatga tattttagat caattgttac
    12961 ctcaattcac agaaagttta atttatgggg ctatcattga tgctaaaacg gctgaacatg
    13021 cagcaggtat gactgccatg cagacagcga cagataacgc taagaatgtc attaacgatt
    13081 tgaccatcca atataaccga gctcgtcaag cagcaattac acaagaaatt actgaaattg
    13141 ttgcaggagc aaatgcacta gaataaaaaa taacgctgac tagcgattag gttattgtca
    13201 gcattcaatt ttatcaatcg agatgaaatg acttatggga cttttctatt gaaagactgc
    13261 tcttttcagt ataaaagaag ttagcaagac taactaagcc ctgactatcg aatataagga
    13321 gaaatgaatg agctcaggca aaattgctca ggttgttggt ccagtagtag acgttatgtt
    13381 tgcgagtggg gacaaacttc cagagattaa taatgcattg attgtttata aagatagtga
    13441 taaaaagcaa aaaatcgtcc ttgaagttgc tctggaactt ggtgacggta tggtgcgaac
    13501 aatcgctatg gaatcaactg atgggcttac acgtgggtta gaagttcttg acactggtcg
    13561 tgcgattagc gtaccagtag gtaaagaaac tttgggacgc gtctttaatg tacttggaga
    13621 aaccattgat ttggaagaac catttgcaga agatgttgac cgtcagccga tccataaaaa
    13681 agcaccatcg tttgatgaat tatcaacatc atcagaaatt cttgaaactg gtatcaaggt
    13741 aattgacctt cttgcccctt accttaaagg tggtaaagtt ggacttttcg gtggtgccgg
    13801 agttggtaag actgtcctta tccaagaatt aatccacaat atcgcccaag aacacggagg
    13861 tatttcagta tttaccggtg ttggtgagcg aacacgtgaa ggaaatgacc tttactggga
    13921 aatgaaagaa tcaggcgtta ttgaaaaaac cgccatggtt tttggtcaga tgaatgagcc
    13981 gcctggggca cgtatgcgtg tagcccttac tggtttaacc attgctgagt atttccgtga
    14041 tgtagaaggc caagatgttt tgctctttat tgataatatc ttccgtttca ctcaggcagg
    14101 ttcagaagta tcagccctct taggccgtat gccttctgct gttggttacc aaccaacact
    14161 tgctactgaa atggggcaat tgcaagaacg gattacatca actcaaaaag gttctgttac
    14221 atctatccaa gcgatttatg taccagcaga tgattatact gaccctgcgc cagcaacagc
    14281 atttgctcac ctggattcaa caactaacct tgaacgtaaa ttgacacaaa tggggattta
    14341 tccagccgtt gatccattgg cttcaagttc acgggcttta tcacctgaga ttgttggtga
    14401 agagcactat gctgtggcaa cagaagttca acgtgtgctt caacgctacc gtgaattaca
    14461 agacatcatc gctattttgg ggatggatga attatcagat gaagaaaaaa cattggttgg
    14521 acgtgcgcgt cgtatccagt tcttcctctc acaaaatttt aacgttgctg aacaatttac
    14581 cggtcttcct ggttcttacg tgccagttgc tgagacagtt cgcggcttta aagaaattct
    14641 tgaaggaaaa tacgatgatc ttccagaaga cgcctttcgt tcagttggtc caatcgagga
    14701 tgtgattaaa aaagccgaaa aaatgggctt ttagtgaggt gatgccatga cacaaatgac
    14761 tgttcaagtg gtaacgccag atggaatcaa atatgatcac catgctaaat gtatttcggt
    14821 aacaacacca gatggggaaa tggggatttt acctaatcac atcaatttaa ttgcaccttt
    14881 gcaagtccat gaaatgaaaa ttcgccgagg tggtgaagat gaaaaagtgg actggattgc
    14941 tatcaatggt ggtattattg aaatcaaaga taatgtggtt accgttgtcg ctgattctgc
    15001 agaacgtgac cgtgatatcg atgtcagccg agcagagcga gcaaaactaa gagcagaacg
    15061 tgaaattgca caggctgaaa cgactcacaa cattgatgaa gttcgtcgtg ccaaggttgc
    15121 cttgcgccgc gctttgaatc gtatcaacgt tagtaaaaag taagtggtgt tgataaaaga
    15181 caaggataag taagaaaggt ttttaaccta ttattttgtt tgctgagtct ttttaacatc
    15241 agtttctgag agtttgacca tactaccttc tctaaacacg ttattattgt tgagaggagt
    15301 tatatggggg aactcttttt tgtatccacg ttgttagtag aaactcagta atgacgctta
    15361 atgtattttt aggctatcaa cttagtcaga gaagatgtca acttaattga taagttggac
    15421 ggatcaagat ggtatgttat aataatccta tggaattatt aatgaagatc attgcctttt
    15481 cgtgtcattt attttttatt ggccttagtt accaattact gattagtgtg attgactgga
    15541 caaaatttag tcattatcat ccagaaaatc ttggccggct acgacttttt gtctttttat
    15601 tagctactgc cctagggtat ttggtgagcc attgtatgct ggaattgctt caaattagcc
    15661 agacgctctt ttttgagttc cgctaatact ggaataaaag aagtatggta taattttact
    15721 acgactaaga gatagataag ggagaaaatt gtggataaaa taatcattga aggtggtcaa
    15781 acacgcttag aaggagaagt tgttattgag ggtgctaaaa acgcagttct tcctttactg
    15841 gcagcatcta ttttaccttc aaaaggaaaa acaattctta gaaatgttcc tattctatct
    15901 gatgttttta ccatgaataa cgtggtacgt ggtttagata ttcgtgttga ttttaatgaa
    15961 gcagctaacg aaatcacagt tgatgcctca ggacacattt tagatgaagc cccttacgag
    16021 tacgtgagcc aaatgcgtgc ctctattgtt gttttaggac caattttggc acgtaatggc
    16081 cacgcaaaag tatcaatgcc tggaggctgt acgattggca gtaggcctat taatttgcac
    16141 ttaaaaggat tagaggctat gggggcaacg attactcaaa aaggtggaga tattaccgct
    16201 caagcagatc gactgcaagg tgccatgatt tatatggact tcccatctgt aggagctacc
    16261 caaaacttga tgatggctgc cactttagct gatggtgtga ccactattga aaatgctgct
    16321 cgtgagcctg aaattgttga tttagctcaa tttttgaata aaatgggagc tagaattcgt
    16381 ggtgctggaa cagagacgtt aacgataaca ggcgtcacaa gtcttcatgg ggtggagcac
    16441 gatgtggttc aagaccgtat tgaagcaggt acttttatgg ttgcagcggc gatgacttca
    16501 ggcaatgttt tgattagaga tgctgtttgg gaacataatc gtccattgat ttcaaaatta
    16561 atggaaatgg gtgtttctgt aacagaagaa gaatatggta ttcgcgttca agcaaacacg
    16621 ccaaagctca aacctgtaac ggttaaaacc ttgcctcatc caggttttcc aacagacatg
    16681 caagctcaat ttacagcttt aatggcagtg gtcaatggag agtctactat ggttgaaact
    16741 gtctttgaaa atcgatttca acatctggaa gaaatgcgac gcatgggctt acaatctgaa
    16801 atccttaggg aaactgccat gattcatggt ggaagacaac tgcaaggtgc tcctgtgatg
    16861 tcaacagatt tacgtgccag tgctgccctt atcttgacag ggattgttgc tcaaggagtg
    16921 acaatagtta ataatttggt tcatttagat cgtggctact atcaatttca tgaaaaatta
    16981 gcaaagcttg gagctactat tagtcgtagc agtgaggtct agcttatgac aaatggttta
    17041 aaatatgtct tagaacaaat gttattactt ttagtaattg ctgccttggc gtgtcttttt
    17101 ttagccattg gacttatgat tggttatagt tttatgggag atggccatag tccatggcat
    17161 attttatcta tggataagtg ggcagagtta gtgaataaat ttactggaaa gtaggccttg
    17221 cctacttttt atagcatttg agaggaagta tgttaacaag gaaaaaacga aaaaggatgc
    17281 ttaattcctt cttattgatt ttgttagtgt tggggtctaa cagccttgct aagagtgatt
    17341 tcatagctag cactcaccct atcaagcaac tttatcagac tcttgttggg gagaagaaaa
    17401 aatcattact cacacataat aatttgcccg ctgatgttcc cacagaagct ctggcggcat
    17461 cggtattgac accatcaatc aaaaagcaac taggtccaga tgttcagtgg aatggagcag
    17521 gcgcctatat tatcaacagg aacaaaacag atttaactgc taaggtagcc agtccgcctt
    17581 atgccaacaa tgaaaccaaa tgggtgcaag agcaaaaggt tcctactaga gctaatgcct
    17641 tacttactaa gtcgacacga cagtatcaaa atcgctatca aacgaacaat gcttatcgta
    17701 cctttaagcc agcaggttgg caccgattgc accacttaaa aggaagttat gatcacgcgg
    17761 ttgatagagg gcatttgcta ggttatgctt tagttggtgg actgaaaggt tttgatgctt
    17821 accagtaatc ctgataatat tgccactcaa ctaagttggg ctaatcaagt aaataaaccc
    17881 tactcgacag gtcagaacta ctatgaaggt ttagtacgtc gtgctttgga taaggggcat
    17941 cgcgtccgct accgtgtcac cctactttat gatggtgata atctactagc cagcggtagc
    18001 catttggagg ctaaatcctc tgatgacagc ttgactttta acgtgtttgt tccaaatgtc
    18061 caaccaggat tgaccgttga ctatcggacg ggacaaatag ctatcaatct ctaagcgaaa
    18121 agtcactgat ttaagattgt cactaagcac tttttttgat agaatagaac taattgaata
    18181 agtgtctatg agactagtac taatagagaa attaacaggg agtgagggcc gtgactggaa
    18241 gccttcttaa gggaactatt aggaattcac tcagctaaag acaattaatt aaggtctgat
    18301 aacatcagat aaaaacggat ggtaccgcgt gtcaacgctc cgcttatgaa gtggagtagt
    18361 ggcacgcttt tttcatcgct aaaagaaggg aaaactatgg atttacaagc acaattagag
    18421 gaattaaaaa caaaaacatt ggagactctc caatctttga cggggaatca taccaaagaa
    18481 ctgcaagatt tgcgcgtggc tgttcttggg aaaaagggat ctttgacaga attactaaag
    18541 gggctaaaag acttgtctaa tgacttgcgt cctgtggttg gtaaacaggt gaacgaagtt
    18601 cgtgacttat tgacaaaagc ctttgaagaa caggctaaaa ttgtagaagc tgctaaaatt
    18661 caagcccagc ttgatgctga aagcattgat gtgactttac cgggtcgtca aatgactctg
    18721 ggccaccgtc atgtgttaac acaaaccagt gaagaaatcg aagatatttt cttaggaatg
    18781 ggttttcaga ttgttgatgg ctttgaggta gaaaaagatt attacaactt tgagcgcatg
    18841 aatttgccaa aggatcaccc tgcccgtgac atgcaagata cgttttatat cacagaagaa
    18901 atcttgttac gaactcacac cagtcctgtg caggcccgta ctcttgatca acatgatttt
    18961 tcaaaagggc ctttaaaaat ggtttctcca ggtcgtgttt tccgtcgtga tactgatgac
    19021 gctacccact cacatcaatt ccatcaaatt gaaggtttgg ttgtgggcaa aaatatttcc
    19081 atgggagacc tcaaagggac tcttgaaatg attatcaaga agatgttcgg tgaagaacgt
    19141 agcattcgtt tgcgcccgtc atatttcccg ttcacagaac catctgttga agtggatgtt
    19201 tcttgcttta aatgtggtgg gaaaggctgt aatgtctgca agaaaacagg atggattgaa
    19261 attcttggtg ctggtatggt tcacccaagt gtcttggaaa tgtcaggtgt tgatgcaaaa
    19321 gagtattcag gctttgcctt tggtttaggt caagagcgga ttgccatgtt gcgttatggc
    19381 attaatgaca ttcgtggttt ctatcaaggc gaccaacgtt tctctgaaca atttaactaa
    19441 aatgagtcgt ttgaagactt ctgtcagcta ttttatctaa gattgagtga aagttgatag
    19501 gtaatgataa taggttaatt gtctaagggg aaagtccctc tcgcttcaac gtcataaggg
    19561 ttttgacttg gaacttaaaa tacgaaagga aatgactcaa atcacggctt gagagctttc
    19621 tctagccgag tgatttgggc actaaaatta tgttagtatc ttataaatgg ttaaaagaat
    19681 tagtagatat cgatgtgaca ccagcagcac ttgctgagaa aatgtcggca acgagtatcg
    19741 aggtagaagg aattgaagtc cctgcagatg gtttatcaaa actggttgtg gggcatgtgc
    19801 tttcttgcga agatgtgcca gagacacatt tgcatttgtg tcaggtagat acaggtgatg
    19861 aaacgccacg tcaaattgtg tgtggcgcac caaatgtgaa agcagggatt aaggttatcg
    19921 tggctgtgcc aggtgctcgt attgcggata actataaaat caaaaaaggc aaaatccgcg
    19981 gcatggaatc tcttggcatg atttgttcct tgcaagaact tggtttgtca gatagtatta
    20041 ttccaaaaga attttcagat ggtattcaaa tcttgccaga ggaggctgtg ccaggtgatg
    20101 ctatttttaa atacctggac ttggacgatc atatcattga attgtccatt acacctaacc
    20161 gtgctgatgc gctttctatg cgaggtgtgg cacatgaagt cgcagctatt tatggcaagt
    20221 cagtatcatt cccccaaaaa aatctacaag aatctgataa ggcaacttca gaagccattg
    20281 aagtggccat tgcctctgat aacgttctaa cttacgctag ccgtgtcgtt gaaaatgtca
    20341 aagttaagcc aagtccacaa tggttgcaaa accttttaat gaatgctggt attcgtccta
    20401 ttaataatgt ggttgatgtg accaactatg tgctcttgta ttttggtcaa cctatgcatg
    20461 cttttgatta tgataaattt gaagaccata agattgtggc gcgtgcggct cgtcaagggg
    20521 aaagtttagt cacacttgat ggtgaaaaac gggatttgac cactgaggat cttgttatta
    20581 ccgtggctga caaaccagtt gctttggcag gtgttatggg tggtcaagcc actgaaattg
    20641 atgccaactc tcagacggtt gttcttgaag ttgctgtttt tgatggcaaa tcgattcgta
    20701 aaaccagtgg tcgtttaaac cttcgttcgg aatcctcttc acgttttgaa aaaggggtta
    20761 actatgcaac tgttttagaa gcgcttgact ttgcagcggc tatgttacaa gaattagcag
    20821 aaggtcaagt cttatcaggc catgttcaag ctggacaatt gccaacagaa cctgttgaag
    20881 tctcaactag ccttgactat gtgaatgttc gtttaggaac agaattaact tttgctgata
    20941 ttcaaaggat ttttgaccaa cttggatttg gtctaacagg agatgaaaca agcttcacgg
    21001 tagctgttcc tcggcgtcgt tgggatgtta gtattccggc tgatttggtg gaagaaattg
    21061 cgcgtattta tggttatgat aagttgccaa caaccttacc agaggcagga ggaacggctg
    21121 cagaattaac gccaacacag gctcttcgtc gtaaggtccg tggtttagcg gaaggattgg
    21181 gcttgactga aatcatttcg tatgccttga caacaccaga gaaagctgtt gagtttgcag
    21241 tggcaccaag tcacctaaca gaactcatgt ggccaatgtc tgtggaacgt tcagcgcttc
    21301 gtcaaaatat ggtttcaggg atgttagata cggtagctta taatgtggct cgtaaacaaa
    21361 gtaacttggc tctttatgaa attggaaaaa tctttgaaca agaagctaat ccaaaagaag
    21421 accttcctaa tgaattgaac cattttgctt ttgctatttg tggcttagtg gcacaaaaag
    21481 atttccaaac ccaagctcag gcagtggatt tttaccatgc taaaggtatc ttggatactt
    21541 tatttgcaaa ccttaacctc aaggttcaat atgtgccaac caaagaccta gcaagcatgc
    21601 acccaggacg gacagcgctt atcctattag atgagcaagt gattggattt gttggtcaag
    21661 ttcatccagg aacagcaaag gcttatagta ttccagaaac ctatgtggca gaattggata
    21721 tggctgcttt agaagcagcg ttgccaagtg atcaaacttt tgcggaaatc acaaaattcc
    21781 ctgctatgac acgtgatgtt gccttgttac ttgaccgtga agtgagtcat caggccattg
    21841 taacagctat tgaatcagca ggtgtaaaac gcttgactaa gattaagctc tttgacgtgt
    21901 atgaaggagt aactattcag gctggcaaaa aatcaatggc ttatagcctt actttccaaa
    21961 atccaaatga caatttaacg gatgaagaag ttgccaaata catggagaaa atcaccaaag
    22021 cattgacaga acaagtcggt gctgaagtac gctaagaaaa aaactgggcc tctaaggctc
    22081 cgtttttttc tgttataata gaggaaaaaa gaactccagt tcaaatgatt ggagaaaaac
    22141 atcagaaggt ggaggaaaat cagaatgtat caaacaaaaa taacaggcaa ccgcctttac
    22201 cacacggttt cgaaaggcta cggagatagt gtaaccttat ttggaaaaac tgatcaaggg
    22261 gaaaccccta tgagtttact ggttattgcc ttgtcttctt gtgtgactat gtgtgtgcag
    22321 ggtttttacc atcgtcaatt tcagcaagac acacttgctg tttctgttga ggctagttat
    22381 gaggaagaag tttttgaact acttgttagg attgatgatg ttttagatga caaaaagaaa
    22441 gctgatttgt tggcttacat tggaacttat tgtcgtgtga aacaactcct caaacctgaa
    22501 gttatggtga ccattacgtt ggaaggactg tctcatgtct gatgctggtt tgaaaactta
    22561 ccaaaaacaa tgggcttatc aaaagtattg ggtcatggca cactcacagc agcattacaa
    22621 tgctttaaga gaattgttta aaggaaatca gtggtcagaa gaaaaggtgt tgacttttca
    22681 ttgtttgatt gaagaagcgc aagccattcc tcccacagta aaaagcttac gaacagcata
    22741 tcagcatgtt tgggggtatt ttaaaaaggt agcgagtcag gaagaaaaag accatttcaa
    22801 agatttggat gctcagttgg aaactaaatc tgaggagatg ctatgctttt tacaagagat
    22861 gacggctcat tatcagccct cttatctgct atcttgccgt ttgattacca aaggtcctta
    22921 aatgctaaac agaaaagtag ggacttctgg gaagttatgc taagctagtg atataaacaa
    22981 gggggaatga gagatgtttt tagcgcttaa tgaaatgaaa caatccaaat tgcggtatgg
    23041 tttgattgct ggtttgctat gtttggttgc ctatttgatg ttctttttgt caggattggc
    23101 ctttggtttg atgcaggaga accgctcggc ggttgatctt tggaaagctg acagtatttt
    23161 actagccaag gatgctgacg ctaccttgac cttatcacag gtgtccagag ctcaagaaaa
    23221 tcaaataaca gcagacaagg tagctcctct tgctcaactc aataccgtgg cgtggtcagt
    23281 taaaaatcct aaggatgccg acaaagttaa ggttagcctt ttcgggattg attctaatag
    23341 ctttattcgt cctaacattg taaaaggtcg attatttaag actaacaaag aggttgtttt
    23401 ggatcaaagc cttgcaaaag aggaagcttt tgcgattggc aaggactttt acacatcgag
    23461 ttcgagtcaa gcattaacta tcgttggtta tactcaaaat gctagattta gtgttgcacc
    23521 agtggtttat atgaatctgg aagcttttga aacattaaaa tatggagaac cactaccaaa
    23581 agataagcaa gttgttaatg cttttatcac taaaggaagt ttaacagatt atcctaaaaa
    23641 agacttccaa aaattagata ttaaaacctt tattactaaa ttacctggtt atagcgctca
    23701 acttttaacg tttggcttta tgattagttt tcttgtcatt atttcagcta ttattattgg
    23761 tatttttatg tatattttga ctattcaaaa ggcacctatt tttgggatta tgaaagcgca
    23821 aggaatttct aacaaaacga ttacgaccgc tgtgcttatg cagacattct ttttgagttt
    23881 tttaggtagt ggtttagggt tgctaggtac ttggctgaca tcattattat taccaacagt
    23941 agttcctttt caaagcaatt ggtttttata tttggctata ttcgttagta tgatctgttt
    24001 tgctctatta ggcaccttat tttctgtttt taacattata cgaattgatc ctttgaaagc
    24061 aattggatag gaggtagtga tgtcggtttt aacatttaaa caagtgacca aaacatttca
    24121 agatggtcat cacgaaatca acgcattaaa ggcaactgac ttttccattg aagctggaga
    24181 atttgtggct attattggac catcaggttc tggaaaatca acctttttga ccattgctgg
    24241 tggcttacag accccaagtt ctggtcagct aatcattgat ggcactgatt atacgcattt
    24301 atcagaaaaa gagcgctcac gcttgcgctt caagtccgtt ggttttatct tacaagcttc
    24361 caatctgata ccttttttaa cagtccagca gcagttggaa ttggtcgatc atttgacagg
    24421 aagcaaagaa aaagccaaag ccaatcagtt atttgacgat ttaggcatta cagggctaaa
    24481 acatcagtta cctcaagaat tgtccggcgg agaaaggcaa agagcggcga ttgcccgtgc
    24541 tctctaccat gatccagcac tgatattggc agacgaacct actgcaagtt tggatactga
    24601 aaaagcctat gaagtggtca aactgttagc taaggaaagt aaagaaaaaa acaaggccat
    24661 tattatggtc actcatgatg atcggatgct aaaatattgt gacaaagtct atcggatgca
    24721 agatggagag ttacgtcaag agagatagta gactggtaag agtttatgat attagaagaa
    24781 aaaaaccaaa caagcacagc acttgtttgg tttttttcta agtgtgacta ttatcaatac
    24841 gtcaccattt ccctaaagct tactatacgg gatgctaaag gtatcaccat gctcaaagtc
    24901 gccatattgg taacctcggt caaaccaacg ttggcgttgc ttagaagtgc catgtgtaaa
    24961 actatcagga acggttctgc cataggtttc ttcttgcaga gtatcatccc caactgcgtg
    25021 agctgctgcc attgcttcct caatatctcc tttttccaaa aggccttgcc cttgaacata
    25081 attagcccag gcaccagcat agtaatcagc ttgtaattcc aacttgacgt tgagctgatt
    25141 ggctttagcc tttgattttc cttgtctagc gctagcgtaa ttatccataa ttcccaattc
    25201 attttggatg tggtggccaa cttcatgagc aatcacatag gccatagcaa aatcaccttt
    25261 tgcgccgtac ttggttgata gttcattata aaaagaaata tctaaataca ctttttgatc
    25321 gcctgggcaa taaaaaggac cagaagaggc ttgacccctg ccacaagcgg tttgagttgc
    25381 tccagtatat agaactaatg ttggtttatg ataagttaac cctttctcac gaaatgtctt
    25441 agtccaatag tcctcggtag aggcgaagac cttactgaca aaagaaacct gttcttgact
    25501 agccttgtca ccatttgtac gcgttacttg gcttgattga taggcattgt tgttggtaga
    25561 agaaggcttg cctcctaaaa cactagatag gccaccccct cccataacta ggagaaggag
    25621 aagaataacc agcttggttt tccaacctcc tcgtgaaaag agtaattgaa gcaggagacc
    25681 gccccctagg ccgccgcccc caaaagaacc agacgattga ccacgtctat cttcaacctg
    25741 ttgactttct ctcaaatcat ccgttttcat aagtttctcc catctatttc ttgattttat
    25801 tataaaataa ttctattaga attgctattt ttatgatgag attataacta aaaataggaa
    25861 aagcaatagg tatttgctat aatataacaa gggtagattc agtttgtata aaggagtcat
    25921 atgaaattaa tttacacaga gatgagttac tcgatgacag agattttggt gaatgaagca
    25981 cgaaaagctg ctgaccaagg ttatcgagtg ttctatattg ctccaaattc tctttcattt
    26041 gaaaaggaaa gagaagtctt aaccttattg cctgaacgag ggactttttc gataatagta
    26101 acccggttcg ttcaaatgtc aaggtatttt acagttgaga gctctccttc taagcaacat
    26161 ctagatgaca cgactttggc tatgattttt tatcgggcct tgatgcaatt aaaaccagaa
    26221 gatttgccga gttatggtcg tttgcaaaat aattcggttt ttattgaaca actagttgag
    26281 ttatataagg aattaaaaaa cgcacaatta agcgtacatg atttaacagg attagaccat
    26341 cctcaaaaac aagaagatct tattaaaatt attgagcttg cagagacgat catgattcag
    26401 caagattata atcaagatag tcctctacaa tcttttgcaa gagcaattaa gttaggactt
    26461 ttaaataacc aactatcaaa aacagttatt gttattgatg gtttttcacg tttttcagct
    26521 gaagaggatt atttattatc acttttaaat aacaactgtc aagaggtcat cattggcagt
    26581 tatgtgagtc aaaaagctta tcaaaaatct tttattaagg gaaatatcta tgaggctagc
    26641 cttcattttt tgcaagattt ggctcaaaaa taccatatca agccggtatt tgcgacctct
    26701 aatcaagtgt ttaaaccagc cttttcacgg ctaacacaat tatttgaagc aactcatgat
    26761 ttttcacaag tagactggca actccaaaag aatgatcttg atcactttag cctttggcaa
    26821 tgtcatcatc aaaaagagga aattgaacat gtagcaaaat ccattcggca aaagctatat
    26881 gaaggttacc gttataagga tatcttagtg ttgctaggag atatggatgc ttatcaatta
    26941 caaattgggc ctatctttga taagtttgaa atcccctact atttaggaaa agcagagcct
    27001 atggctgccc atccattagt ccaatttata gaatcgctcg aacgaagtca gcgctataat
    27061 tggcgtcgtg aagacatttt aaacatgtta aaatcaggac tttttggttg ttttgatgat
    27121 agtgatattg atcgctttga ggaatatact cagtttgcag atattaaagg atttactaaa
    27181 ttttcaaagc cttttaccat taactcttct aggcagtatc ctcttgactt tttaaatgaa
    27241 atgagacaag acatcgtttt acctttgcaa gaattgttta agagtcaaaa acaacttggt
    27301 gcttcacttg ttgacaaatt aatacttttt tttaaaaaga ttagattagc tgagaatatg
    27361 caaggtttag cacagtccca attagaggtg gaaaaaaacg aagaagtttg gaaaaggttt
    27421 acagatattt tgacttcttt tcatcatatc tttggccaag aaaaattacg gctatcagat
    27481 tgccttgctt taatcaaaac gggaatgaaa tcagctcagt atcgtgttgt tccagcgaca
    27541 ttagatgttg tcactattaa atcttatgac cttgttcaac cacattcaaa acctttcgtt
    27601 tatgcaattg gtctaactca atcacatttt cctaagcaaa ttcaccattc aggactactt
    27661 tctgatcaag agcgtgctag aattaatgaa ataagaaatt atcgtcattt tgatattgct
    27721 agtgccgaaa atagtaaaaa aaaccatcaa actgcgttat ctctgtttaa tgctgctact
    27781 aaagagttag ttcttagtgt tccaacggta atcaacgaga cattcgatga tctatctcct
    27841 tatcttaaag aattaataag ctttgggcta cctcttttgg ataaaggcaa aaactatcta
    27901 tcttatgata atagcgatat tggtaactat aaagctctct tgtcacaaat tattgctatt
    27961 aaccgacaag atcttataga aatgtcagat caagataaga tgttttggac tgttgtctta
    28021 cgttatctca ggaaacagtt aagaaagcaa cagctagagc tacctacttc agattatcgt
    28081 ttatcaacaa agcctttgtc taaagaagtt atcgaggtat gttttccaaa gggaatccct
    28141 ttaaagctat ctgcaactgc tttaaccgtt ttttataata atcagtacaa ctattttttg
    28201 aaatatgttt tgaatttaaa caaaacagaa tccattcatc cagattccag aattcatggt
    28261 caatacttgc atcgtgtctt tgagcggtta atgaaagatc acacgcaaga accgtttgat
    28321 aataaattaa aacaagcaat ctaccatacc aatcaagaaa gtttttttca gcaggtttac
    28381 caagataatg ccgaggctga gtacagttta gctatactag aagatattgt tagatcaaca
    28441 gcacctatct tgcaacttaa ccaaaacata aaggtcattg atcaagaaaa gaactttcat
    28501 ctggatatgg gaaatgagat attagtccac gggatcattg atcgtattga ccagctgagc
    28561 gacggtagct tgggcattgt tgactataaa tcaagcgcga atcaatttga tattgggacc
    28621 ttttataatg ggttgagtcc acaactagtg acttacctcg cagccttaaa acagatcgca
    28681 ccgcacgata taaaccaatt atttggggct atgtaccttc atttgcaaga tcctaaattg
    28741 gatctagtaa catttaaaca aattgataac acattagtgg agagtatcta taaagcgttg
    28801 acctataaag gtattttttc agaagtcgaa aaagagcatc tatctactgg tgcttaccaa
    28861 accaaaaatg ctctttattc caatgacgaa ttggaaacac ttttaaatta taacaagtac
    28921 ctctatctta aagctgctaa acacattaaa aagggacatt ttttgattaa tccttataca
    28981 agcgatggta agacagttca aggagatcaa ttaaaagcca ttacccgttt tgaagcagat
    29041 cttgacatgg cacaggcaag gcgactagtg actctccctg ctaaggagaa aaaagagtgc
    29101 tttttaacat taatgagaaa ggagagccac ttgtgatttc ttttgcccca tttttaagcc
    29161 ccgaagctat taaacattta caagaaaacg aaaggtgcag tgatcagtct caaaaacgca
    29221 cagctcaaca aattgaagca atttatacta gtggccaaaa tatacttgta tcagcttctg
    29281 ctggttcagg aaaaaccttt gtaatggtcg aacgcatact tgataaaatt ttgagaggtg
    29341 tttcaattga tcggcttttt atctcaacct ttactgttaa agcagctaca gaactgcgtg
    29401 aacggattga aaacaaatta tactcacaaa ttgctcaaac tacagatttt caaatgaaag
    29461 tttatttaac agaacaattg caatctcttg gtcaagctga tattggtact atggatgctt
    29521 ttgcacagaa agtagtaagt cgctatggtt atagcattgg catttcatcc caatttcgta
    29581 tcatgcagga taaagcagaa caagatgttt taaagcaaga ggtgtttagc aaactcttta
    29641 gtgagtttat gaatcaaaaa gaggcaccgg tgtttagggc tcttgtgaaa aatttttctg
    29701 gtaactgtaa agacacttca gcttttagag agttagttta tacttgttat tcttttagcc
    29761 aatcgacaga aaatccaaaa atatggttgc aagaaaattt tctaagcgct gctaaaactt
    29821 accaaaggct tgaagatatc ccggatcatg atattgaact cttacttttg gcaatgcaag
    29881 acactgcaaa tcagctaaga gatgtgactg atatggaaga ttatgggcag ctgactaagg
    29941 caggtagccg atctgctaaa tacactaaac acttaacgat catagaaaag ttgtctgatt
    30001 gggtgcgtga ttttaaatgt ttgtatggaa aagccggatt ggatcggttg atcagagatg
    30061 tgacaggcct tataccatct gggaatgatg ttacagtctc gaaggtaaaa taccctgttt
    30121 ttaagacctt gcatcaaaaa ttaaaacaat ttaggcattt agaaacaatt ttaatgtatc
    30181 agaaagactg ttttccctta ttggaacagt tacaagattt tgtgcttgcg ttttcagaag
    30241 cttatttagc tgtcaaaata caagaaagtg cttttgaatt ttcagatatt acacactttg
    30301 caatcaaaat tttagaggaa aatacggata ttcgccaatc ctatcagcaa cactatcatg
    30361 aggtgatggt tgatgaatat caagataaca atcatatgca agagcgactc ctgaccttac
    30421 tatcgaacgg tcataatcgc tttatggtag gagatatcaa acaatcgatc tatcgatttc
    30481 ggcaagccga tcctcagatt tttaatcaaa agtttagaga ctatcaaaaa aaacctgagc
    30541 aggggaaagt gattttactc aaagaaaact ttcgtagcca atcagaggtg ttaaatgtca
    30601 gcaatgctgt ttttagtcac ttaatggacg aatcagtagg agacgtctta tacgatgagc
    30661 aacatcagtt aatagcaggt agtcatgctc aaacagtccc ctatctagac cgtcgtgctc
    30721 agttattgct atataatagc gataaagatg atggcaacgc cccttcagat agtgagggta
    30781 tttcatttag tgaggttaca attgttgcca aagaaattat taagcttcac aatgataagg
    30841 gtgtcccttt tgaagacatt acgttactcg tttcttcaag aacaagaaat gatatcattt
    30901 ctcatacatt caatcaatat agtattccta tagtaacaga tggtgggcag caaaactatc
    30961 ttaaatctgt tgaagtgatg gttatgttag atacattacg caccattaat aacccaagaa
    31021 atgattatgc ccttgtggct ttactgcgct caccgatgtt tgcctttgat gaggatgatt
    31081 tagcaagaat agcacttcaa aaagacaatg agctagataa agattgccta tatgacaaga
    31141 tacaaagggc tgtgattgga agaggtgctc atcctgaatt gattcacgat accttgcttg
    31201 gcaagttaaa tattttttta aagacgttga aaagttggcg tcgatacgct aagctagggt
    31261 cgttgtatga cttgatttgg aaaattttta atgatcgttt ttattttgat tttgtagcta
    31321 gtcaagcaaa agcagaacaa gcacaagcta atctatacgc attagctcta cgtgctaatc
    31381 agtttgaaaa atcgggctat aaagggctat accgttttat taaaatgatt gataaggtac
    31441 ttgagacgca aaatgactta gcagatgtgg aagtggctgc tcctaaacaa gctgttaatt
    31501 taatgaccat tcacaagtct aaaggtttac aatttccgta tgtatttatc cttaattgtg
    31561 acaagcgctt ctcaatgaca gatattcata aatcatttat tctgaatcgg cagcacggta
    31621 tcggtatcaa gtaccttgca gatatcaaag gtttacttgg tgaaacaaca ctcaattctg
    31681 ttaaagtaag catggaaacc ttaccttatc aattgaacaa acaagagttg cgcttagcaa
    31741 ctttatcaga acaaatgcgc ttattgtatg ttgctatgac acgagctgaa aaaaaagttt
    31801 attttattgg taaagctagt aagagcaaaa gtcaagatat cacagatcct aaaaagttag
    31861 gcaaactttt gccgctggct ttacgagaac agttattgac attccaagat tggctattag
    31921 caatagcaga tatattttca actgaagatc tttattttga tgttcgcttt attgaagata
    31981 gtgatttgac acaagagtca gtcggacgac ttcaaacacc acagttatta aatccagatg
    32041 atcttaaaga taatcgtcaa tcagaaacaa ttgcacgggc tttagatatg ttagaagcag
    32101 tgtctcaatt gaatgccaat tatgaagcag ctattcattt gccaacagtt cgaacgccta
    32161 gccaacttaa ggtagcttac gagcctttat tagaacccat tggtgtagat attatagaga
    32221 aatcttctcg atcgctatct gattttactt tgccacattt ttcaaaaaaa gtaaaagttg
    32281 aagcaagtca tattggatca gctcttcatc agttgatgca ggtgctccct ttgtcaaaac
    32341 cgataaatca acaaacgctt ttagacgctt taagagaaat tgatagtaac gaagaggtaa
    32401 aaacagctct tgatctcaaa aaaatagagt cgttcttttg tgatacaagc ctaggccaat
    32461 tttttcagac ttaccaaaaa cacttgtatc gagaagcacc atttgctatt ttaaaagttg
    32521 accctattag tcaagaagaa tatgtcctac gtggtattat agatgcctac tttttgtttg
    32581 atgatcatat tgtattagtg gactataaaa cagataaata caagcagccc attgagttaa
    32641 aaaagcgtta ccaacaacag ttggagttat atgcagaagc tctcactcaa acgtataaac
    32701 ttcctgtgac taagcgctat cttgttttaa tgggaggtgg aaagccagaa attgtcgaag
    32761 tttaaggttg ttgtgacaca agggtatttt gcaaatgata atagtatagt tgaggatttt
    32821 acgaaatgct tgaaaagcta ttgacctttg aataataata caatataata agttttatta
    32881 gtgacaaaaa ggaataaata tactatgaat tttaaaaaaa taagatttgg ttttttgctt
    32941 tgcctcctac tttatccttt agcagcttgt aataagtctg aacaacttaa tcactacgaa
    33001 agaattaaaa aaacaagaaa attagtcgtt gctgtaagcc ctgattatgc tccttttgaa
    33061 tttaaagctt tagttaatgg aaaagatact attgttggag cagatgttca attagctcag
    33121 gctattgctg atgaattaga cgttgatctt gagctttcac caatgagttt tgataatgtt
    33181 ttgtctagtt tacagactgg taaagctgat cttgctattt caggaatctc acatactaaa
    33241 gaaagagcta aagtatatga tttttcaatt ccttactatc aggcagaaaa cgctatagtg
    33301 atgagagcat ctgatgctaa agtgaccaaa aatattagtg acctaaacgg taagaaagta
    33361 gccgctcaaa aaggtagtat cgaggaaggt ttagttaaaa tacaattacc aaaggccaat
    33421 ctgatttctt tgactgctat gggagaagcc attaatgaac taaaagcagg acaagtctat
    33481 gcagttacct tagaagcacc tgtagctgct ggttttttag cccaacataa ggatctggct
    33541 ttagcaccct ttagcttaaa aaccagtgat ggagatgcca aagcagtggc ccttcctaaa
    33601 aatagtggtg acttaaccaa agccgttaat aaggttattg ctaagttaga tgagcaagaa
    33661 cggtacaagt catttattgc agagacaata gcactaacga aaaatactat gaagtaataa
    33721 ggttaataaa aactttatta gtatccgttg gaaaagtgtt tagaataaac aataaaagag
    33781 gt
//
LOCUS       DATUYR010000014        33144 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.014, whole genome shotgun sequence.
ACCESSION   DATUYR010000014 DATUYR010000000
VERSION     DATUYR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33144
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.014"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            13..852
                     /locus_tag="VCI33_000610"
     CDS             13..852
                     /locus_tag="VCI33_000610"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEP2831551.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            932..1429
                     /locus_tag="VCI33_000611"
     CDS             932..1429
                     /locus_tag="VCI33_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEP2831552.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAVL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            1449..1619
                     /locus_tag="VCI33_000612"
     CDS             1449..1619
                     /locus_tag="VCI33_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831553.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLGSLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            1749..2978
                     /gene="clpX"
                     /locus_tag="VCI33_000613"
     CDS             1749..2978
                     /gene="clpX"
                     /locus_tag="VCI33_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEP2831554.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            2988..3587
                     /locus_tag="VCI33_000614"
     CDS             2988..3587
                     /locus_tag="VCI33_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEP2831555.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPKDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDNSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            3735..4484
                     /locus_tag="VCI33_000615"
     CDS             3735..4484
                     /locus_tag="VCI33_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831556.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKKSVKCIT"
     gene            complement(4537..6636)
                     /locus_tag="VCI33_000616"
     CDS             complement(4537..6636)
                     /locus_tag="VCI33_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP2831557.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATTSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKERQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGFNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILEEGQLVIRQKDQ"
     gene            7014..7697
                     /gene="rpiA"
                     /locus_tag="VCI33_000617"
     CDS             7014..7697
                     /gene="rpiA"
                     /locus_tag="VCI33_000617"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEP2831558.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEVLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEVPKAS"
     gene            7774..8985
                     /locus_tag="VCI33_000618"
     CDS             7774..8985
                     /locus_tag="VCI33_000618"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEP2831559.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTVPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGSRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDIA
                     MIGESFLGHLK"
     gene            9001..9441
                     /gene="arsC"
                     /locus_tag="VCI33_000619"
     CDS             9001..9441
                     /gene="arsC"
                     /locus_tag="VCI33_000619"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEP2831560.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLTDPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYIKEDGEIVNPIEVKGEKDVIDD"
     gene            9425..10234
                     /locus_tag="VCI33_000620"
     CDS             9425..10234
                     /locus_tag="VCI33_000620"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP2831561.1"
                     /translation="MSLMTKINETKDFLVAKGIEAPEFGLILGSGLGELAEEVENPVV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     THSGLKVLGISAITNFAAGFQSELNHEEVVEVTEHIKEDFKGLVKAILAEL"
     gene            10819..11532
                     /gene="deoD"
                     /locus_tag="VCI33_000621"
     CDS             10819..11532
                     /gene="deoD"
                     /locus_tag="VCI33_000621"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP2831562.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGRRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVALETLIAND"
     gene            11525..12313
                     /locus_tag="VCI33_000622"
     CDS             11525..12313
                     /locus_tag="VCI33_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEP2831563.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRIIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKTELGLWRQSIV"
     gene            complement(12392..13297)
                     /locus_tag="VCI33_000623"
     CDS             complement(12392..13297)
                     /locus_tag="VCI33_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP2831564.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLNMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHSKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            13519..14055
                     /locus_tag="VCI33_000624"
     CDS             13519..14055
                     /locus_tag="VCI33_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831565.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     EAFIKYYQSPKVPTGEFE"
     gene            14332..15024
                     /gene="pyrF"
                     /locus_tag="VCI33_000625"
     CDS             14332..15024
                     /gene="pyrF"
                     /locus_tag="VCI33_000625"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEP2831566.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRSIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            15082..15711
                     /locus_tag="VCI33_000626"
     CDS             15082..15711
                     /locus_tag="VCI33_000626"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEP2831567.1"
                     /translation="MTLGSQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            15908..17362
                     /locus_tag="VCI33_000627"
     CDS             15908..17362
                     /locus_tag="VCI33_000627"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEP2831568.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKLFAGVPIFLKDLGQELKGDLSTSGSKLFKNHHATKTDLFVK
                     RLEDLGFIILGRSNTPEFGFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKNSIYQSLQRPLTIAFYQRLSDGSPVSLDTEKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            17480..18343
                     /locus_tag="VCI33_000628"
     CDS             17480..18343
                     /locus_tag="VCI33_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP2831569.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWETIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DAQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPIIDTKDVN"
     gene            18372..19022
                     /locus_tag="VCI33_000629"
     CDS             18372..19022
                     /locus_tag="VCI33_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP2831570.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFLTGLVFGLLLALLGR
                     SHQPLLHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            19155..19808
                     /locus_tag="VCI33_000630"
     CDS             19155..19808
                     /locus_tag="VCI33_000630"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEP2831571.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGIDWLQ"
     gene            19932..21200
                     /locus_tag="VCI33_000631"
     CDS             19932..21200
                     /locus_tag="VCI33_000631"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEP2831572.1"
                     /translation="MLLIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAASGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLIVGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(21258..21899)
                     /gene="plsY"
                     /locus_tag="VCI33_000632"
     CDS             complement(21258..21899)
                     /gene="plsY"
                     /locus_tag="VCI33_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEP2831573.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            22034..23983
                     /gene="parE"
                     /locus_tag="VCI33_000633"
     CDS             22034..23983
                     /gene="parE"
                     /locus_tag="VCI33_000633"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEP2831574.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKTEKVSYAWTDGEL
                     EDLRKEFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            24074..26533
                     /gene="parC"
                     /locus_tag="VCI33_000634"
     CDS             24074..26533
                     /gene="parC"
                     /locus_tag="VCI33_000634"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEP2831575.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKDADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKTDAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            26611..27678
                     /locus_tag="VCI33_000635"
     CDS             26611..27678
                     /locus_tag="VCI33_000635"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEP2831576.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            27742..27972
                     /locus_tag="VCI33_000636"
     CDS             27742..27972
                     /locus_tag="VCI33_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEP2831577.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            28033..28113
                     /locus_tag="VCI33_000637"
     tRNA            28033..28113
                     /locus_tag="VCI33_000637"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28067..28069,aa:Tyr,seq:gta)
     gene            28164..28235
                     /locus_tag="VCI33_000638"
     tRNA            28164..28235
                     /locus_tag="VCI33_000638"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28196..28198,aa:Gln,seq:ttg)
     gene            28362..29567
                     /gene="rpsA"
                     /locus_tag="VCI33_000639"
     CDS             28362..29567
                     /gene="rpsA"
                     /locus_tag="VCI33_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEP2831578.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            29680..29751
                     /locus_tag="VCI33_000640"
     tRNA            29680..29751
                     /locus_tag="VCI33_000640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29713..29715,aa:Arg,seq:ccg)
     gene            complement(30114..30416)
                     /locus_tag="VCI33_000641"
     CDS             complement(30114..30416)
                     /locus_tag="VCI33_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831579.1"
                     /translation="MTYELCLEYGTYPLSRVYAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            30616..31602
                     /locus_tag="VCI33_000642"
     CDS             30616..31602
                     /locus_tag="VCI33_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEP2831580.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQGWAERLEAIGESLPEFVGA"
     gene            31913..32317
                     /locus_tag="VCI33_000643"
     CDS             31913..32317
                     /locus_tag="VCI33_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEP2831581.1"
                     /translation="MLFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMY
                     NPHRYTISQDNQALEKVKQASYKRMDIAMTHLDGLIGDSGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLARQTHEETFVFRKLDS"
     gene            32363..>33144
                     /locus_tag="VCI33_000644"
     CDS             32363..>33144
                     /locus_tag="VCI33_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element IS1548 family transposase"
                     /protein_id="HEP2831582.1"
                     /translation="MIDFIISIDDCAVELDSRQSWKIRYPLSTILFLVFVCQLAGIET
                     WKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVISLVNTDRLKELKVQFEQSLTSLD
                     AVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLR
                     TIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEE
                     LQENAQYYQTVEKSRGQIEVREYWVSSDIKWLCQNHPKWHKLR"
BASE COUNT        10364 a         5818 c         6947 g        10015 t
ORIGIN      
        1 aggttattag agatgacaaa agctgaccag atttttaaag ctaatattca aaaaattata
       61 aacgaaggat cattgagtga gcaagcacgt cctaaatata aagatggtcg aacagctcat
      121 tctaagtata ttactggggc gtttgctgaa tatgatttag ctaaaggaga gtttcccatc
      181 acaactttac gacccattcc aattaaatct gctataaagg aattattttg gatatatcaa
      241 gatcagtcca acagtttaga tgtgctagaa gctaaatata atgttcatta ttggaatgaa
      301 tgggaagttg accagacacg tactattggg caacgttatg gtgctgtagt caaaaaacat
      361 gatatcatct caaaaatact aaagcaatta gcagaaaatc cttggaatcg tcgtaatgtc
      421 atttctctct gggactatga agcatttgag gagacaaaag ggttgttgcc atgtgccttt
      481 caaatcatgt ttgatgttag acgtgtaggg gaagatcttt atttagatgc tagtttgacg
      541 caacgttcta atgacatatt agttgctcat cacatcaatg ccatgcaata cgttgctttg
      601 cagatgatga ttgctaaaca ttttggatgg aaaataggta agttctttta ttttgttaac
      661 aatttacata tttatgacaa ccaatttgat caggctcaag aattactaaa gcgacagcca
      721 gttgctagtc agcctaagct tgttttaaat gttccagatg ggactaattt ctttgatatt
      781 aaaccagatg attttgaatt acaaaattat gatccagtga agcctcagtt gcactttgac
      841 cttgctattt aaactttatt tagatttttt gtgagcattt tgttcataaa tttggtaaaa
      901 taaatagagt ttaaaatgaa ggaaataatt aatgacaaag gaaattattg ctatttgggc
      961 agaggatgaa gcagggctta ttggtgttgc gggtaagtta ccatggtatt taccaaaaga
     1021 gttagaacat tttaaaaaga caacgctaca tcaagcagtc ttaatgggcc gtgtgacttt
     1081 tgaaggaatg aattgcaaaa gacttcctca acgtcaaacc cttgtcatga caagaaatag
     1141 agattatcaa gtagatgagg tgttgacaat gactagtatt gaaaaagtct tggagtggta
     1201 ccatgcgcag gataaaacac tttatatcat tggtgggaat aaggttttag aagcgtttaa
     1261 tggttatttc gatcgtatca taaaaacagt tatccatcat cgttttaaag gagataccta
     1321 ccgaccaaat ctagatttta gtcatttcac gcaagaaagc caaacgttct atgctcgaga
     1381 cgctaaaaat ccttatgatt ttaccgttac tgctttaaaa cacaaatagt ctgaggagat
     1441 gataagcgat gcaacgtagt atttttggag tatttacggc ttttttgtgc gtgatttgtg
     1501 ttctttgtgc catcccagct tttaaaaaga atcgttatgg cttaggaagt ctgttcttat
     1561 taaatgcgtt tactaatctg gttaatagta ttcacgcctt ctatggtaca ttgttttgag
     1621 atgtgacttg cgaagacagc actggtgaag atttagctat caagcaagaa tcactaaaaa
     1681 ccaatacaac acattgacta atattaaaat tcagtaaaag ataagagaaa gtaagagaat
     1741 tataagaaat ggcaggaagt agaactaacg atattaaagt atactgttca ttttgtggta
     1801 aaagccagga tgacgttaaa aaaattatcg caggaaataa tgttttcatc tgcaatgaat
     1861 gcgtagcttt atcacaagaa attataaaag aagaattggc tgaagaagtc cttgccgatt
     1921 tgacagaagt tccaaaacca aaagagctcc ttgatgtttt aaatcaatat gtggttggtc
     1981 aagatcgtgc taaacgtgct ttatcagttg ctgtgtacaa tcattataag cgtgtctcgt
     2041 ttacagaaag tcgtgatgac gatgatgtgg atttacaaaa gtccaacatt ttgatgattg
     2101 gaccaacagg ttcaggaaaa accttcttag cacaaactct ggctaaaagc ctcaatgtac
     2161 catttgctat tgcagatgca acttctttga ctgaagcagg gtatgttgga gaagatgtgg
     2221 aaaatatttt actcaaactg attcaggctg ctgactataa tgtcgaacga gcagagcgtg
     2281 gtattattta tgttgatgaa atagataaga ttgctaaaaa aggtgagaac gtatctatca
     2341 cacgtgatgt atctggtgaa ggtgtacagc aagcccttct taaaattatt gaaggtacgg
     2401 tagcaagtgt tcccccacaa ggtgggcgga agcaccctaa tcaagaaatg atccaaattg
     2461 atacaaaaaa tatcttgttt attgttggcg gggcttttga tggcatcgag gagattgtta
     2521 aacaacgttt aggtgaaaaa gttattggtt ttggtcaaaa cagccgtaaa attgatgaca
     2581 atgcttctta tatgcaagaa attatttcag aagatattca aaagtttggg cttattccag
     2641 aatttattgg ccgcttacca gttgtggcag cccttgaaca actaaacaca tcagatttga
     2701 ttcaaatttt gacagaacca agaaatgccc tggtcaagca atatcaggcc cttctttctt
     2761 acgatggcgt cgaattggca tttgataagg aagccttaga agccattgcc aataaagcca
     2821 ttgagcgtaa gacaggagca cgtggtcttc gttctattat tgaagaaacg atgttagaca
     2881 ttatgtttga aattccaagc caagaagatg tcacaaaagt gcgtatcacc aaggctgctg
     2941 tcgaaggcaa gtcaaaaccc gttttagaga cagcttaagg agaaataatg gctgaagaac
     3001 aagtcttaaa tacccataac gcctcaattt tattgagtgc tgccaataaa tctcattacc
     3061 ccaaagatga tttgccagaa attgccctcg ctggacgttc aaatgttggt aaatcaagtt
     3121 ttattaatac catattgggg cgcaaaaatt tggcaagaac ctcaagcaag ccaggaaaaa
     3181 cccaactatt aaattttttc aatattgatg acaagcttag atttgtggat gtccctggtt
     3241 atggctatgc caaggtttct aaaagtgaac gggccaaatg gggaaagatg atcgaagaat
     3301 acttaaccag cagagacaat ctgcgtgcag tcgtgagttt ggttgattta cgtcatgccc
     3361 cttcaaaaga agatattcag atgtatgact ttttgaagta ctatgacatt cctgttattg
     3421 ttgtggcaac caaagcagat aagattcctc gtggcaaatg gaacaaacat gagtctgtgg
     3481 tcaagaaagc tttgaatttt gacaactctg atacttttat agtattttct tcggtagaac
     3541 gtattggtat cgatgattct tgggatgcta ttttagagca ggtctagcga taggtgttga
     3601 tttatctatg ctaaaacatt ctcagtagag ggtgtttttc ttatcgaaaa cataaaaaat
     3661 catagttagg tttaccgatt ttacttgacg ccttaatgat gacatgctag tattttatat
     3721 taaggagatg agtcatgaca caagtagccc aagggcgctc taaaggaaag tcattgttat
     3781 gccatcaatg caatgccctt tttgtattta ttatttttct tatcttgctt gctgtacttt
     3841 ttggtgttcg atatcgtaat agctctattg aaggtatctg gcgaacaaca agcattgatc
     3901 agaagttagg cgatgatttt gctaaacgtc ttactggttt gcatcagagc cctcttattg
     3961 atgatagttt attaacgtca tctcagatga tactaacagt taaaaataac aacgttgact
     4021 tgagttttag tgttcaagtt gaacgagaca tttttgtcaa acgtttagct gcttatcacc
     4081 aaaatgaatt attaaagaca ttaaaggaaa atcatttagt tgttggtgac ttgagtagta
     4141 aagaacgcca aattattgag aatagtatgc cagctagcca tgaattagaa atgattttag
     4201 atcaagcttt tgagaaatta gcttctcaga tcggaggcaa atataatcaa aaaacaggac
     4261 atttatctgc tgttgttctc aaaggaaaag ttaatcgtat tttgcacacc attgatatca
     4321 aagaagaagt agcagcggga catacttctt tttcaaaagg attgctcaca cctaatggtt
     4381 attttgatta caccagattt ggaaagaaac ttgagttatt aggcgatgaa aaaattattt
     4441 ttaaaaaagc gctaaaaaaa aagtccgtca agtgtataac gtagtcacaa gacggatttt
     4501 ttcatgtttt gaaaattatt caattgaaga agtttattac tgatcttttt gcctaatcac
     4561 tagttgtcct tcttctagga ttgcctgaag ttttttgact tctggatggt caagataata
     4621 atcagttata cggtcacgaa tttcctgttc gataatacgt cgtaatggac gagctcccat
     4681 agcgtgattg taccctaaat ctatcaaatg agctttagcg tcatcagcaa tcgttaaact
     4741 aatacctttt tttgcggtgg tttgatttac ttcagctagc attaaatcaa caatttcttc
     4801 taaattttct ttttgcagat gattaaattt aatgatacta ttaaaacggt tcaaaaactc
     4861 tggtctgaaa taaggtgcaa tgcgatccat aatgttaaac ccagaagttt ccgtatcttg
     4921 ttggccaaaa ccggcatttg atgtagcaat aataacggta tttttaaagt taatagtatt
     4981 tccttgtcca tctgtcaatc gtccatcatc taacacttgt agaagaagtg taatgatttg
     5041 aggatcagct ttttcaattt catctagtaa gacaatagca taagggttac gacgaacacg
     5101 ctcagttagg gtattattat tatcatcgta gccaacataa ccagccgttg tcccaatcaa
     5161 ttttgacaca gcagtgcgat cgttatattc ggacatatca agtcgaataa tagcatcttt
     5221 ggaaccaaat aaatcaagtg ctaattgttt agctaactct gttttaccaa caccagttgg
     5281 acccacaaaa aggaaagaac ctattggacg gttaccatca tcaaaacctg ctcgatttcg
     5341 tcgaatagca cgtgctactg ctgcaacagc ctcatcttgt ccgatgacat ggctttttaa
     5401 gcggctgcta attcctttga gcctttctaa atcattagtc cccatgttag agacgggaat
     5461 accagttaag cgctctacag actctgcgat atcattgact gtagcagtaa cattttggct
     5521 ttggttatgt tgctcaattt gcttttgtaa tgtttcaatc cgtgtttttg ctgctaaagc
     5581 tttttcaaag tcttctttgg cgacagcttt ttcttgcctc tctttttgtt tagctatttc
     5641 agtttcgagt gtttttaagt cagtgaccgg gtgttgcgca gctaaatgag cagctgtcat
     5701 atccagtaag tcaattgctt tatctggcag gctacgctgt gggatgtatt gaatagaata
     5761 atcaacagca gcttttagaa cattatcagg aagggtaata tggtggtgtt gttcataaag
     5821 attacgaata cccattaaaa tgtgaaaagt atcttcagct gatggtgcat tcactttaac
     5881 ttcattaaag cgacgagcga gtgcagcatt ttttaaaata gtattgcggt attcgtcttg
     5941 agtagtagca ccaatcagtg ttaattctcc tcgtgacaag gctggtttaa gaatatctgc
     6001 aagtcccttt gagcctgaat cactgcttgt ggcaccagca ccaactattt gatgaatttc
     6061 atcaaaaaat aagatgatat tcccagcttc ttttacttct tgaatcaagt tttgaatggt
     6121 ctcttcaaag gaaccacgat actgtgtacc agcttcaaga ctagaaatat caattgatac
     6181 gatttccttg tttttaatag cagctgggac atctccatta acaatggctt gagctaaacc
     6241 ttcaatgact gcagttttac cgacaccagc atctccgaca aggacaggat tatttttagt
     6301 tcgtcgtgcc aagatttcag cggtatcttg aatttctttg ttacgaccaa tgacggggtc
     6361 aagatgccct tgacgagctt cttgtgttaa gttagttccg agttgagtca atacactatc
     6421 agctttaggt gttaacattt gagaagttgt tgctttggtt gtcgctacag ggagttgtcc
     6481 tgtttgacga tagtgttgga attcttcggg agttatttcg cgaccgttaa cgagatagcg
     6541 gggattttcg ctacgataac cacccatgtt tgccattaat tggttgaaaa tatcatccat
     6601 atttacaaaa ggatctttac cagaaaaatg tgtcatatca gttacctcgc aataaaaaat
     6661 agactataag atgtttgtaa gcagtcgcag aatcgttgtt aggatttcac atgataggaa
     6721 accctttgct aataaaatga ttcagacatt atttttactc atcttatagt attattataa
     6781 ccaaaaggtc agtaaaggtc aaacaaaaac acttgatttt caaaaagcta aataaacacg
     6841 aaaagtaagt gcttatatga tgactttaga ataaatcata agttttaaaa agatcatcct
     6901 attctatagc acttaggctg tcaatgagag aaaaacaaat gttttcatta acaagttggc
     6961 gctttcatgg tataataaag tgctaagacg caattctatt ataaggggga atcatggaag
     7021 cactcaaaaa aattgccggt gttaccgctg cgcaatatgt gactgatggc atgaccattg
     7081 ggcttggaac tgggtcaacg gcttactatt ttgttgagga aattggccgt cgcgtaaagc
     7141 aagaaggact gcaagtggtt ggtgtgacta cgtcaagtgt gactagcaag caagcagaag
     7201 tcttaggcat tcctttaaaa tcgatagatg atattgatag cattgacctc accgtagatg
     7261 gtgctgatga agtggataag gatttcaatg gaatcaaggg tggcggcgca gccttactaa
     7321 tggaaaaaat tgttgcaaca ccaacaaaag aatacatctg ggttgttgat gcttctaaaa
     7381 tggtagaaca tctgggtgcc tttaagttac cagtcgaagt cgttcaatat ggtgctgatc
     7441 gtttgttccg tgtctttgaa aaagctggtt acaaaccgtc ttttcgtatg aaaggtgata
     7501 gtcgtttggt gacagacatg caaaactata ttattgatct tgatttgggt tgtattaaag
     7561 atcctgttgc ttttggtcac ctgttagatg gaacagttgg tgttgtggaa cacggtctct
     7621 ttaatggtat ggttgataag gtgattgttg ctagtaaaga cggcgttact gttttagaag
     7681 tgcctaaagc aagctagtct tagcatataa cagtgttaat gtattttata ggtattgggg
     7741 agtcaaccgt aacctcccct agattggaga cttatgtcaa aatttaatcg tattcacttg
     7801 gttgttttag actctgtcgg tattggtgca gcaccagatg ctgacaaatt ctttaatgct
     7861 ggtgtagctg atacagattc tgacaccctc ggtcatattt cagaaacagc aggcttatcg
     7921 gttccaaata tggcaaaaat tggacttgga aacatttctc gtccaatacc actaaaaact
     7981 gttccaacag aagataatcc tactggttac gtgaccaaat tggaagaagt atctcttgga
     8041 aaagatacca tgacaggcca ttgggaaatt atgggtttga acattacaga accttttgac
     8101 actttctgga atggtttccc agaagagatt ttaactaaga ttgaagaatt ctcaggtcgc
     8161 aaaattattc gtgaagctaa taaaccctat tcaggaactg cggttattga tgattttggt
     8221 tcgcgtcaaa tggaaacagg tgagttgatt gtttacacct cagcagaccc agtgcttcaa
     8281 attgcagccc atgaagacat tattcctgtg gaagagcttt acaagatttg tgaatatgct
     8341 cgctccatca cgcttgaacg ccctgccctt ttaggccgta ttattgcgcg tccttatgta
     8401 ggcgaaccag gaaactttac acgtacggct aaccgtcatg actatgcagt gtctcctttc
     8461 caagataccg ttttgaacaa actggctgac gcaggtgtcc caacttatgc cgttggtaag
     8521 attaatgaca tctttaatgg ttcaggtatt acaaacgaca tgggacacaa taagtcgaac
     8581 agccatggta ttgacacgct tattaagact cttcaattac ctgagtttac taagggattc
     8641 tcctttacta acctcgttga ttttgatgcc aactttggtc atcgtcgtga cccagagggt
     8701 tatcgcgact gcttgcatga atttgacaat cgcctacctg aaatcatcgc aaatatgaaa
     8761 gaagacgatt tgcttctcat cactgctgac catggtaatg acccaaccta tgcaggcacc
     8821 gaccacactc gtgaatacat tccattgctt gcctactctg catcctttac tggaaatggt
     8881 cttattccac aagggcattt tgcagatatt tcagcgactg ttgcagaaaa ttttggagtt
     8941 gatatagcca tgattgggga gtcattcctt ggtcacttaa aataagaaaa ttagttgata
     9001 atggaagaaa tcacgattta tcataatcca aactgtggca cttcacgtaa tgttcttgcc
     9061 atgattcgcc atgcaggtat tgagcctact attattgagt atttgcagac gccacctaat
     9121 cgggagactt taattgagct cttacaatcg atgggaatct ctgcgcgaga actcttgcga
     9181 accaatgttc ctgaatttga ggctcatgga ttagcaaatc aagcagtagc tgagaaagac
     9241 gttattaatg ccatgttgac ggatccaatt ttgatcaatc gtcctattgt tgtgactcgc
     9301 aaaggagtaa aactttgccg tccttctgag accttactgg atattcttcc tgttcccctg
     9361 cctagtcctt atattaaaga ggatggcgag attgtgaacc ctattgaggt aaaaggagaa
     9421 aaagatgtca ttgatgacta aaattaacga aacaaaagac tttttagttg ctaaaggaat
     9481 tgaagctcca gaatttggat taattctggg ttcaggactt ggtgaacttg ctgaagaagt
     9541 agagaatcct gttgttattg attatgcaga tattccaaac tggggcaaat caacagttgt
     9601 tggccatgct ggaaaattag tctacggtga tttagctggt cgtaaagtac ttgccctaca
     9661 aggtcgtttc catttttacg aaggaaaccc gcttgaagtg gtgactttcc ctgttcgtgt
     9721 catgaaagct ctgggatgtg aaggtgttct tgtcacaaat gcagctggtg gtatcggcta
     9781 cggcccaggt accttgatgg caatcacaga ccatatcaat atgaccggaa ataacccact
     9841 aatcggtgaa aacttggacg agtttggtcc acgcttccct gatatgtctg atgcctatac
     9901 taaggtttat cgcaataagg cacatgaagt ggctgaaaaa atgaacatca aactagaaga
     9961 tggtgtttat atgggcttaa ctggtccaac ttatgagaca ccagcagaaa tccgtgcttt
    10021 caaagtatta ggagcagatg ctgtcggcat gtcaactgtg ccagaagtta ttgtggctac
    10081 tcattctgga cttaaagtac ttggcatttc agccatcact aactttgcag ctggtttcca
    10141 atcagaattg aaccacgaag aagttgttga agtgactgaa catattaaag aagatttcaa
    10201 aggtcttgtt aaagctattt tagctgagtt gtaggacgaa caaaatcaac tggagcatta
    10261 ttacaggaat gttattttat gtcgtcagtt tggcacatct gttcgcaata aaaaaatcaa
    10321 aaaataatca cggggtgtga gttccatgaa tgttgaactc agattctagc ttttctaact
    10381 aacataacca acgaagggaa agagacgtat tcggtttaaa ttaagcatgg actcgaatat
    10441 ttcttcgtat taatttttaa ataataaagg aaagagagtt agtcctttct tatttgaact
    10501 cgatttttag ctctcttctt ctattcaata tcaattatga aaggaatggg gttgtattca
    10561 attgaatacg ggcggaaagc tcggaaacta gataaactaa cttttgtgct cacacatggc
    10621 gttagttccc taaatttcag ttgctttctg tccgccctct gaccttctac aaaataaaag
    10681 gaaaggtagg gctaactgta ttcattgaat tgaatacggg cggagaactg tgtaaaaaag
    10741 ataaactgtc tagcatctgg gatgcgtcgt cagtttccta tttttacttt gttctttgtc
    10801 gcctttaata tcttaataat gtctatccat atttctgcca aaaaaggtga tattgctgat
    10861 aaaattcttc tacctggaga ccctttgcgc gcgaaattta tcgctgaaaa tttcctagaa
    10921 gatgcagtgt gttttaacga agtgcgtaac atgtttggct acacaggaac ttacaaaggg
    10981 cgccgcgttt ctgtcatggg aactggtatg gggatgcctt cgatctctat ctatgctcgt
    11041 gaattaatcg ttgattacgg tgtcaaaaca ttgatccgtg ttggtactgc gggtgcgatt
    11101 gacccagaag ttcatgtgcg tgaattggtg cttgcccaag cagcagcaac caactcaaat
    11161 attatccgta atgacttccc agaatttgat ttcccacaaa ttgctgattt tggtttactt
    11221 gacaaggctt accatatcgc cagagaaatg ggagtaacaa ctcacgttgg gaatgtgctt
    11281 tcttctgatg tcttttacac taacatgcct gagcgtaata tggctcttgg taaattgggt
    11341 gttaaagcca tcgaaatgga agcagcagct ctttactatt tagctgctca gcaccatgtc
    11401 aaagcccttg gaatcatgac gatctcagat aacttgaatg atccaaccga agatactaca
    11461 gctgaagagc gccaaaccac tttcactgat atgatgaagg tcgccttgga aaccttaatt
    11521 gccaatgact aataatcaaa cactagacat ccttttggat gtctatgctt ataatcacgc
    11581 ctttagaatt gctaaagcct tgccaaatat ccctaaaact gccctctatt tactagagat
    11641 gttaaaagag cgcagagaat tgaaccttgc ctttctggcg gaacatgcag cagagaatcg
    11701 gatcattgaa gaccagtacc actgttcatt atggcttaac caatcgcttg aagatgagca
    11761 gattgccaat tacattttgg atttagaagt taaagtaaaa aacggtgcta ttattgattt
    11821 cgtcaggtca gtgtcgccta ttctttaccg actttttctc agactaatca cgtcagaaat
    11881 tccaaacttc aaggcttata tttttgatac aaagaatgac caatatgata cctggcattt
    11941 tcaggccatg ttggaatctg atcacgaggt ttttaaggct tacctgtctc aaaagcagtc
    12001 tcgcaatgtg acgaccaaaa gcttagcaga catgttgacg ttgacctcct tacctcagga
    12061 aatcaaggac ttggtttttt tgttacgaca ttttgaaaag gctgtccgta atcctctggc
    12121 tcatttgatt aagccttttg atgaagagga actgcatcgc accactcatt tttcttcaca
    12181 ggcttttttg gaaaacatta tcaccttggc gactttttct ggtgtaatct accgacgtga
    12241 acctttttac tttgatgaca tgaatgccat tattaaaacg gagttgggcc tttggagaca
    12301 atctattgtc tgatatttat caaaaaacac gataacttga ccgatacagg tcttactacc
    12361 gtgtttttta gtgtgacgat tgaaaaagaa attaatcaaa ggttacttct tcgagaaggt
    12421 attcgataaa acgttccccc attttggaca agttggcttt ttcatgcttg ataaaaacaa
    12481 tgtcaatttc gtcagggaca tctagcggga tagccacaat ctggtcacca tttagattgc
    12541 tatttagaat accactggca acggtgtagc catctaaacc aatcattaag ttaaagaggg
    12601 tggcacggtc actgaccaca attgatttgc tatggggcat ctgggacatc atctcttctg
    12661 aaaaataaaa ggagttgtga atcccttggt catagctcag gtaaggaaaa tctctcaagt
    12721 cgtccatgtt taataggctt ttagttgcta gaggatttga tttgctgaca aaaatatgag
    12781 gctgtgcctt aaataatgga gaggcagtca aatgattgtc atcaaataat ttggtcaaga
    12841 catctcggtt atagtcgttg ataaagagaa caccaatttc agatcgaaag tttttgacat
    12901 catcaatgat ttcccaagtt cttgtctcac gcaaaaagag ttcgtaccga gtcatgtcgg
    12961 tccgcttgag gagtgagaca aatgcattga ctacgaaggc ataatgttga gatgaaacgc
    13021 taaagagctc tcggccagta ttgtggtttt tatagcggtc ttctaacagt gatgtttgct
    13081 ctataatttg gcgagcataa gataaaaatt ccaccccatc ttttgtcaag gtgatccctt
    13141 taggatttcg gttgaaaatg gtaatgccca tctccatttc caaatccttg accgcattgg
    13201 atagacttgg ttgtgtgata aataattgct tagcagcttc attcatagag ccgcactcaa
    13261 caattttgat aatataatgc agttgttgaa ttctcataag tacagtctag ctcaaaaaaa
    13321 ataaagacgc aagcacaaac gtcatcatgg gtttagaact ttgaaaaagc gctggagtct
    13381 tttctcgata tggcctagga taaatatgat tcaattttga gggttgaatc tatcaatttc
    13441 attgcccttt tatttttctt gtgttatatt aatagtatta acaaataggt atttttctaa
    13501 tcaaagggtg gtggtcgtgt gaatgttact gaacaaaata gtcatcaaat cttaatccaa
    13561 aagttattgg tcagtatcca ctatttgact ctttttcgag atgaactgaa gcttgtagaa
    13621 cgaactcctt ctatacttgg aggggaattt ccagctcatt tagtacagtc tgagttggga
    13681 gatattgttg cggccattga taccttggat atgcagcaac gcttgattga gtctaccttt
    13741 tggtatgaag aatcagcttt taagttgatg aacaaaactt tggatattgt cgataattgg
    13801 attaaaggag tggatcatct gatagattta tgccagtcca aagaagtctt tcaaattatt
    13861 attggagata aacgcattcg agtttttggg gtcttaagtg atgttttttc atctttaaaa
    13921 gtgagtgctt tgtcacttaa ggaagctcct attccggatg tgctctatga acatatccag
    13981 agggttaatc ttgaagaaga agcttttata aaatattacc aaagccctaa ggtacccaca
    14041 ggtgagtttg aataaatgat tgggtaaaaa tgataaaatg agttgacgta agtgtacaaa
    14101 aaaagatata gtaggaaaaa tgtgttcaaa taacaaaaag ttttgacatc ttgttttaaa
    14161 aaagctataa tagtttaaat ttagacacct ttaagtgagt ccagagaggc aaacaaggta
    14221 tacaagataa aaaaagggaa ttccctttta gtttgctgta gcctcgcttc tttggtagcg
    14281 aggctttttg gtgcttaatg agatattgac acattataga aaggataagt tatgaaagaa
    14341 gaacgcccaa ttattgcttt agacttttca tcatttgagg agactaaagc atttttagac
    14401 ttatttccag cagaagaaaa actatacgtg aaaattggta tggaattata ttatgcgcaa
    14461 ggaccagata ttgttcggtc tatcaaatcg ttagggcata atgtattttt ggatttaaaa
    14521 ttacatgata ttccaaatac tgttcgtgct gctatggcag ttcttaaaga attagatatt
    14581 gatatggcaa ctgttcatgc agctggtggt gtagagatgc taaaagcagc tcgagaagga
    14641 ctaggccaag gccctacctt aattgcggta acgcagctaa cttcaaccag tgaagaccaa
    14701 atgcgtggtg atcaaaatat tcaaacgagt cttttagagt ctgttttgca ttacagcaag
    14761 ggggcagcta aagctcagct agatggggtg gtttgttcgg cacaagaagt agaagctatc
    14821 aaggcggtga cgcctacggg atttacttgc ttaactcctg gcattcgtcc taaaggcagt
    14881 aatattggcg atcaaaaacg agtgatgact ccaaaccaag caagacgcat tggaagtgat
    14941 tatattgtgg tagggcgacc aattacccag gcaaaagacc cagtggctgc ttaccaagcc
    15001 ataaaagctg agtgggcagg ctaacagata agattaggaa accaacactt gtctgttaaa
    15061 gaagaaaaag gagaataaga aatgacacta ggaagccaaa ttgcaactca attacttgat
    15121 attaaagctg tttatttaaa gcctgaagat ccttttacgt gggcatctgg cattaaatca
    15181 cctatttaca ctgataatcg tgtcaccctt tcttatccta agactcgtga tttaattgag
    15241 aatgggtttg tggaaactat cagggctcat ttcccagaag ttgaggtcat cgctggtaca
    15301 gctacagcag gtattcctca cggtgccatt atagcagata aaatgactct tccttttgct
    15361 tatattcgca gtaagccaaa agatcatgga gcaggcaacc agattgaggg gcgtgtgctt
    15421 aaaggccaaa aaatggttat cattgaagat ttgatttcaa caggtggttc ggtactagat
    15481 gcagccgcag cagccagtcg tgagggagct gacgttctgg gagttgttgc gattttcact
    15541 tatgagttac caaaagctag tcagaatttt aaagaagctg gtatcaagtt gattacgctt
    15601 tctaattata ctgagctaat tgcagttgca aagttgcaag gctatattac taatgatggt
    15661 ttacatttac ttaaaaaatt taaagaagac caggtaaact ggcaacaata aggaactatc
    15721 agccaggttt atttttaatg aaaaaatctc tttaacccca attcaaaatg ttatgttttg
    15781 attgttttgt ctacattcct tacacatcac attctaattt ctaacctttt aatggtggtt
    15841 ttcttttatt tgtgttttaa taaaaaagaa tgttttagga ttcatatctt agaaaggaag
    15901 agtagctatg acttatcaag atgcgacggc tatggctatt gctgttcaga ccggtcagac
    15961 cacaccacta gaattagtaa cccaggctat ttataaggct aaaaagttga accctacctt
    16021 aaatgctatt acctcagaac gctttgaagc agccctagaa gaagccaaac aaagggattt
    16081 ttcaggtaag ctttttgcag gagttcccat ttttttgaaa gatttgggac aagaattgaa
    16141 gggagactta tcaacatcgg gttctaagct attcaagaat catcatgcga ctaaaacaga
    16201 tttattcgtg aaaaggttag aagacttggg atttattatt ttagggcgtt ctaacacacc
    16261 tgaatttggc tttaaaaata ttagtgatag tcagctccat ggcccggttt cattacctca
    16321 tgataataca cgaaatgctg gtggctcatc tgggggagct gctgctctag tgtcatcagg
    16381 aattagtgca ctagcaactg ctagtgatgg tggtggttct attcgaattc cagcctcgtt
    16441 taatggctta attgggctca aacctagccg cgggcgcatg cctgtcggac ctggttctta
    16501 tcgaagctgg caaggagcct cagttcattt tgccttgacc aaatccgtta gagatactag
    16561 aaatctactc tactatttac agatggaaca aatggaaagc cccttccccc tagccacctt
    16621 aacaaaaaat agtatttatc aatcgctaca acgtcccttg acaattgctt tttatcaacg
    16681 gttaagtgat ggtagcccag tatctttaga tactgaaaaa gctttaagac aagcagtaac
    16741 ttggttaaga gaacaagggc atcagctagt tgaattggaa gagtttcctg taaatatgac
    16801 agaagtgatt cgtcattatt atattatgaa tagtgtggaa acggctgcta tgtttgctga
    16861 tattgaagac acttttggac gtccgatgac caaagacgat atggaaacca tgacctgggc
    16921 tatttaccaa tcaggtaaag acatcccagc ttggcgttac tcacaggtcc ttcaaaaatg
    16981 ggatacgtac agtgcaacca tggctagttt tcatgagact tatgacctgt tgttgacgtt
    17041 cacaaccaac acaccagctc ctaagcacgg agaacttgtc ccagattcga aactcatggc
    17101 taacttggct caagctgaaa ttttcagcag tgaagagcaa ttcaatttgg tcgaaaccat
    17161 gtttggtaag agtctggcta ttaatcctta cacagctctg cctaatctaa ctggccagcc
    17221 agctattagt ttacctactt acgaaactaa ggaagggctc ccaatgggca ttcaactaat
    17281 tgcagccaaa ggtagagagg acctattatt aggaattgca gagcagtttg aggcagcagg
    17341 attattgaag attcctcaat aataatctta taaaacagtt taactattat agattaactt
    17401 tttaaagaga ttgagcgctc actgataaca caacttaatc aaaatgttat actattctta
    17461 tcttaataaa ggagttacta tgaagaaatc agttacttta ctgtctatcg gacttgccag
    17521 ccttttattg gcagcatgtg cgccacataa atctcaaaaa tcaagttggg agactatcaa
    17581 agaaaaaggg gtgttaaaag tagctacccc aggaacctat cagccaactt ctttttacaa
    17641 cgataataat gagttagtag gttacgaagt agatatggtc aaggaaatcg gtaaacgact
    17701 taacattaaa gtcaagtttg ttgaaacagg atttgaccaa gcctttacct cagttgatag
    17761 tggtcgagtg gatatttctt tgaacaactt tgacattacc ccaaaacgtc agaaaaaata
    17821 caatatctct acgccttata aatacggggt aggaggcatg attgtccgtg ctgatggcag
    17881 ctcaaacatg gctaaaaaag atcttagcga ctggaaagga aaaaaagcag ctggcgcttc
    17941 tggaacagag tacatgaaag tagcgcaaaa acagggagct gaactggtga cctacgataa
    18001 tgttacagga gatgtttatc taaacgatgt ggctaacggt cgaacagatt tcattccaaa
    18061 tgattaccca gctcaaaaat tatttgtgga ttacatgtta tctcaaaatc caaatctaaa
    18121 tgtcaaaatg agcgatgctc agtacaaccc aaccgaacaa ggaattgtta tgaacaaaaa
    18181 agacgatagc ctcaagaaaa aaatagatgc tgttattaaa gacatgataa aagatggtag
    18241 cttgaagaaa atatctgaaa cctactatgc tggtcaagat ttgacaaaac cttttggcaa
    18301 agacaaaaaa attcccatca ttgatacgaa agacgttaac taatacttga tgttaaagga
    18361 gtcctaacca catgattaat atcccattaa tgaaagatag tttaggattt gtattatcgg
    18421 gattacctta tacccttggc atatcgttgc ttagcttttt aacggggctg gtctttggtt
    18481 tgctccttgc cctactagga aggtcacatc agccactgct acactatctt gttagggcct
    18541 acatctctat tatgcgaggt gtgccgatga tcgtggtgct ctttgttctt tatttcgggt
    18601 tgccttatta tggtttagaa ttgccagctt tactttgcgc ttatcttggt ttttctatgg
    18661 ttagcgctgc ctatatttct gaagtttttc gctcttctat agaagctatt gacaagggac
    18721 agtgggaagc agctaaggct ttagggttgc catatgctct tatggttaag aaaattattc
    18781 ttcctcaagc ctttcgaatt gcagttcctc ctctaggcaa tgtcattatt gatatggtta
    18841 aaagttcgtc gctagctgcc atgattaccg taccagatat ttttcaaaat gccaaaatta
    18901 tcggtggtag ggagtgggac tacatgtcca tgtacatttt agtagccttt atctattgga
    18961 ttattgcttt tttgttagaa cgttaccaag aattcttaga aaataagcta gcacttgttt
    19021 aatatcctgt aaaaaacgaa tattaaccct ggaaatatca tattttcagg gtctttctat
    19081 ttaaaatgtg gtaaactaga tagaaaagga catcgtattt aacttagaaa ctgacgtgat
    19141 tagaaaggaa tcttatggct cattcaattt ggcatgagaa aatcaaatca tttttgccgg
    19201 agcattacta cggtcgcatc aatcactttt tagatgaagc ctatgcttcc ggtcttgtct
    19261 atcctccacg agaaaatgtc tttaaagcct tacaagtcac tcctttggaa gaaaccaaag
    19321 tgttgatttt aggacaagac ccttatcatg gccccaaaca ggcccagggc ttatcatttt
    19381 cagttccaga agagatttct gctccgccat cccttattaa tattttaaaa gaattagcag
    19441 atgacattgg tcctcgtgac catcatgatt taagcacttg ggctagtcaa ggggttctgc
    19501 ttttgaatgc ttgcttaaca gtgccagcag gccaggctaa tgggcatgca ggcttaatat
    19561 gggagccatt tacagacgct gttattaaag tgctgaatga gaaagacagc cctgtagttt
    19621 tcattttgtg gggagcttat gcaaggaaga aaaaagcctt cattactaat ccaaagcacc
    19681 acatcatcga gagtcctcat ccaagccctt tgtcgtctta tcgtggcttt tttggcagca
    19741 aacccttttc aagaacaaat gctattttag aaaaagaagg catgactggc atagattggt
    19801 tacaatagga caagcattag tgattatcag gcagatggtt tgaggtaagc agcctatgga
    19861 caaagtttca gtattattgg cagacaggtc tgtctatcag ttttacagat aagtgagaag
    19921 aaggagagag catgttactc atcaaaaatg gacgcgttat ggacccgaaa tcgcagagag
    19981 atcaagtggc agatgtcttg attgatggta aacagattgt aaagatagct tcagctattg
    20041 aatgccaaga agctcaagtg attgatgcta gtggtcttat tgttgctccg ggtttggtcg
    20101 atattcatgt gcattttaga gaacccggtc aaacgcacaa agaagatatt cacacgggag
    20161 ccttggcagc agcatcaggt ggtgtgacta cggttgtcat gatggctaat accaatcctg
    20221 tcatttcaga tgtcgaaact ttacaggaag tattagctag cgcagctaaa gaaaagattc
    20281 atatttacac caatgccagt gtcacccaag cctttaatgg aaaagacgtg actgatttta
    20341 aagctttgtt ggaagcagga gcagtaagct tttcagatga tggtattccc ttagaaagtt
    20401 ctaaagtttt aaaagaagcc tttgatttgg ctaatgccaa ccaaactttc atttctttgc
    20461 atgaggaaga tcctcagtta aatggggttc ttggttttaa tgaaggtatt gctgaggagc
    20521 attttcattt ttgtggggca actggtgtag cagaatatag tatgattgca agagatgtca
    20581 tgattgctta tgacaggcaa gctcatgttc atatccaaca tttatctaaa gcagaatcgg
    20641 ttcaagtagt ggcttttgct cagcagttgg gtgctaaggt aactgctgaa gtgtccccac
    20701 aacatttttc aacaactgaa gatctcttat tgattgtagg taccagcgcc aagatgaatc
    20761 ctccccttcg tacacaaaga gatcgcttag ctgtcataga aggcttgaaa tcgggtgtta
    20821 taacagtcat tgccactgat catgctcctc atcacaagga tgaaaaagct gttgatgata
    20881 tgactaaagc tccatctggt atgacaggac ttgaaacttc tttgtcatta ggtttgacgc
    20941 atttggttga accaggtcat cttacactta tgtcactatt agaaaaaatg acgcttaatc
    21001 cagctttgct ttatggtttt gatgcgggtt atttggcaga aaatggtcca gcagatttgg
    21061 ttatttttgc agacaagcaa gaacgtctga tcactgagaa ttttgcttct aaagctagta
    21121 attccccatt tattggtaac aaactcaaag gtgtcgtgaa atacactatt gcagacggag
    21181 aggttgttta ccctaattaa caaatgagtt aaaacaaaaa agcttgactt tcagaggaag
    21241 tcaggctttt agtaaattta ttttttaggc acttgtttgc ttaaatttag cccccaaggt
    21301 attagatttt cagtatgatg tttgatacga ctaatgttat ctttgtgtct tataataata
    21361 atgaacgcaa gtaatatcac aataaaagtt aaaaagtagt cataatttgg taaaagaaaa
    21421 tgaatggcag gaaatgttaa aacagataac acaccaacga tagctgaaac cacactagct
    21481 aaagatatca tgctaaataa atagagaact aaaacaaaga tagatgctaa aaaaaagaga
    21541 tataacggag caaagcctag caatacacca gcacttgttg ctacggcctt accaccttta
    21601 aagttggcaa aaataggaaa agtatgccct aaaactgcga aaaagccgat agcaatggat
    21661 gaaattgaag tcataccaaa aataattggt aacaatattg aaagtgtccc tttaaacata
    21721 tcaatagcta aggtagctgt tcctgccttg acacctaaaa tccgaaaagt atttgtggtt
    21781 ccagtatttc ctgatccatg ctctcgtaag ttgatgtggt aaaagtactg tccaatccat
    21841 agtccagttg gaatagaacc aagtaaatag gcaatggtaa taaaaagtag taatttcata
    21901 atccttatta taacatattc atagattgga gaactagatc tggaaaagaa ttcggaaaaa
    21961 attagcaaat tattttgcaa aattggtaaa aaacttgtaa gataaaaaag atgaaactgt
    22021 ctggaggtcg gtgttgacta agaaagaaat tacaattaat aattataatg acgatgctat
    22081 ccaagtccta gaagggttag atgctgttcg caagcgtcca gggatgtata tcggttcaac
    22141 agatgctacg ggattgcacc acctgatttg ggaaattgtt gataatgctg tcgatgaggc
    22201 cttgtctggt tttggagatg atattaaggt tgttatcaat aaagatggct cggtcagtgt
    22261 ggcagatagc ggacgtggaa tgccaacagg tcagcatgcc atggggattc ccaccgttca
    22321 agttattttt acgattcttc atgcaggtgg taaatttggt cagggtggct acaaaacgtc
    22381 aggtggtttg cacggtgttg gctcctctgt tgtcaatgct ttgtctgcct ggttagaagt
    22441 cgaaatcaca agagacgggt cagtgtatcg tcagcgcttt gaaaatgggg ggaaacctgt
    22501 tacaaccctt aaaaaagttg gcacagctcc taagtctaaa tcaggaacgg ttgtaacctt
    22561 tatgccagat gataagattt tttcaactat tgactttaag ttcaatacca tttcagaacg
    22621 attgaaagaa tctgcttttc ttttgaaaaa tgtcaagatg agcttgacag atcttcgtgg
    22681 agatgacccg attattgaag agtttcatta tgaaaatggg gttcaagatt tcgtcgaata
    22741 cctcaatgaa gataaggaaa ccttgacacc tgttatttac atggaaggtc aagaccaaga
    22801 ctttcaagtc gaggttgctt tgcagtataa cgatggtttc tccgacaata tcttatcctt
    22861 tgtaaacaat gtgcgcacca aagatggtgg aagccatgaa acgggactaa agtcagctat
    22921 taccaaagcg atgaatgact atgctcgtaa aaccaatctc ttaaaagaaa aggataaaaa
    22981 tctagagggc tcagattatc gagaaggact ctcagcagta ttatctatct tagttcctga
    23041 gcagcacttg caatttgaag gacagaccaa agacaaattg ggaagtccct tagcgagacc
    23101 tattgtagag agcattgttt ctgaaaaatt aaccttcttt ttactcgaaa atggagaagt
    23161 agcttctcat ctagttcgca aagccattaa agctagagat gctcgcgaag ctgctcgaaa
    23221 agcgcgtgat gactcacgta atggtaagaa aaacaaaaaa gacaaaggct tgttgtcggg
    23281 aaaattaacc ccagcccagt ccaaaaatgc caagaaaaat gaattgtatc tggtcgaagg
    23341 agattctgcg ggaggttcag ccaaacaagg acgagaccgc aaatttcagg ctattttacc
    23401 attacgtggc aaagttttaa ataccgaaaa agctaagatg gctgacatct tgaaaaatga
    23461 agaaattaac actatggttt ataccattgg agctggagtg ggagctgact ttaacctcga
    23521 ggatatcaac tatgataaga ttattatcat gaccgatgct gataccgatg gggctcatat
    23581 tcagactctt ttgctgactt tcttttaccg ttatatgcga ccgttggtgg aagcaggtca
    23641 cgtttatatt gcgcttcccc cgctttataa aatgtcaaaa ggtaaaggta aaacagagaa
    23701 agtgtcctac gcttggacag atggtgaatt agaagattta cgtaaggaat ttggtaaagg
    23761 agctattctt caacgctata aaggactggg tgaaatgaat gctaaccaat tatgggaaac
    23821 cactatggac cctgaaacca gaacccttat tcgtgttact attgacgact tggcaagagc
    23881 agagcgacgt gtttctgtgt tgatgggtga caaagcagcc ccacgccgtc agtggatcga
    23941 ggataacgtc aagtttacct tagaagaaaa tacggtgttt tagtcataca agtttctcac
    24001 agataattac aagtgctaaa gacgattgtt ttttaggtaa ttaataaata atgaaaaaga
    24061 aagctatttg aatatgtcaa acattcaaaa catgtccctt gaggatatta tgggagaacg
    24121 cttcggccgt tattccaagt atattattca ggaacgggca cttcctgata ttcgagatgg
    24181 attgaaaccc gttcagcgtc ggattcttta ttcgatgaat aaggatggca atacttttga
    24241 aaaaggttac cgtaaatcag ccaaatcagt tggtaatatc atggggaatt tccacccgca
    24301 tggggattcc tctatttatg atgccatggt acggatgagt caaaattgga aaaaccgtga
    24361 aatccttgtt gaaatgcacg gaaataatgg ttcaatggat ggcgatccgc ctgcggctat
    24421 gcgttataca gaagctcgct tgtctgaaat tgcaggttat cttcttcaag acattgaaaa
    24481 aaatacggtc tcttttgcgt ggaactttga tgacacagaa aaagaaccca ccgttttacc
    24541 agcagctttt cctaacttac ttgtcaatgg gtcttctggt atttccgcag gctatgcgac
    24601 agatattcca ccgcacaatt tgtcagaagt gattgatgct gttgtgtata tgattgatca
    24661 tcccaaggct agcttagaga aattgatgga atttttgcct gggccagatt tcccgacagg
    24721 aggcattatc caaggagctg acgaaatcaa aaaagcctac gaaacaggta agggacgtgt
    24781 cgttgttcgc tctaggacgg aaattgagga acttaaaggt ggtaaacaac aaattattgt
    24841 cactgagatt ccttatgaag ttaacaaagc agtcttggtc aaaaagattg acgatgttcg
    24901 tgttaacaat aaagtcccag gcatcgtaga agtacgtgat gaatctgatc gcacaggtct
    24961 tcgcatagct attgagttga aaaaagatgc agatagccaa accattctaa attatttgtt
    25021 gaaatacaca gaccttcaag ttaattataa tttcaatatg gttgccattg atcacttcac
    25081 accacgtcaa gtagggcttc aaaaaatact atcaagctat atcagtcacc gcaaagatat
    25141 tattattgag cggtctaaat tcgataaagc caaagctgaa aaacgcctcc acattgttga
    25201 aggattgatt cgtgtcttgt ccatcttaga tgagattatt gctttgatta gatcgtctga
    25261 caataagact gatgctaaag aaaatctgaa agtcagctat gatttctctg aggaacaagc
    25321 agaagcgatt gtaaccttgc agctttatcg tttaactaat accgatattg ttactctcca
    25381 aaatgaagaa aatgatttaa gagacttaat cacgaccctt tccgctatta tcggtgacga
    25441 agcaaccatg tataacgtca tgaaacgtga attaagagaa gtgaaaaaga aattcgccaa
    25501 tcctcgttta tcagagttac aagcagaaag tcaaatcatt gagattgaca ctgccagctt
    25561 gattgcagag gaagaaactt ttgttagtgt gacacggggc ggatacctca aaagaaccag
    25621 cccacgatcc ttcaatgcgt ctagtttgga agaagttggc aagagagatg atgacgaact
    25681 catcttcgtt aaacaggcca aaacaacaga acaccttttg ttgtttacaa ctttaggcaa
    25741 tgtcatttac cggccaatcc atgaattgac cgatttacgt tggaaggata tcggtgaaca
    25801 tttatcacaa accatttcaa acttcgcgac agaagaagag attctctacg ctgatattgt
    25861 gacttccttt gaccagggct tgtatgtggc tgtcacacaa aatggtttta tcaaacgctt
    25921 tgatcgtaaa gaactaagtc cttggcgaac ttataaatct aaatcaacca aatatgtgaa
    25981 gctaaaggat gacaaagacc gcgtggttac cttgtcacca gttattatgg aagacctcct
    26041 attagtgact aaaaatggtt atgctttgcg attctccagt caggaagtgc ctattcaagg
    26101 gttgaaatct gctggtgtta aaggcattaa cttgaaaaat gatgacagcc ttgcctcagc
    26161 ctttgctgtg acaagtaact ctttctttgt cttaactcag aggggttctc tcaaacggat
    26221 ggctgtcgat gatattcctc agacaagtcg tgccaatcgt ggactattgg tcctacgaga
    26281 gttaaaaacc aaacctcata gagtttttct ggccggaggc gttcaatcag atacttcagc
    26341 tgagcagttt gatttgttca ctgatatacc tgaagaagaa actaatcaac aaatgcttga
    26401 agtcattagt aagactggtc aaacctacga gattgcttta gaaacgcttt cgttatcgga
    26461 aagaaccagc aatggctcct ttatttcaga cactatttcg gatcaagaag tactggtagc
    26521 tagaacacgt taaaaagagt gaaaactctt tttaatttta tggtaaattt agaatattaa
    26581 tgcaaaagag tagaataaac ttgaaaatta ttgaaaaaaa gttacaatag aaaaaagcag
    26641 aaaagaggta cgatgatgac aatagcaatt gattgggaca acctaggatt tgaatatcat
    26701 aagttgccat ttcgttacat ttcttattac aaaaatggtc aatgggataa aggtcaatta
    26761 acagaagatg ccaccttaca tatctcagaa agcgctccag cgcttcatta tgggcaacaa
    26821 gcttttgaag gtttaaaggc ttatcgcacc aaagatggct ctattcaact atttagacca
    26881 gaccgtaatg cagtgcgttt acaagccaca gcagaccgct tgttgatgcc acaagtatca
    26941 acagagcaat ttattgatgc agctaagcaa gtggttaaag ccaatgaaga ctttgtacca
    27001 ccttacggaa caggtgcaac gctttacctg agaccgttat taattggagt tggagatatt
    27061 attggggtta aaccagccga agaatatatt ttcaccattt ttgccatgcc agtaggaaac
    27121 tatttcaaag gtggacttgc tccgactaat tttattgttt ctgaagcgtt tgaccgcgcc
    27181 gctccttacg gaacaggtgc tgctaaggtg ggaggaaact atgcaggaag tttgctacca
    27241 ggtaaggccg ctaaatcagc aggcttttct gatgtgattt acttagatcc agcaacccat
    27301 actaaaattg aagaagtcgg agctgcaaac ttttttggca ttactgcaaa taatgaattt
    27361 gttacgccac ttagtccatc tatcttgcca tctattacga agtattcact cttacaacta
    27421 gctgaagaaa gacttggtat gacagtaatt gagggtgatg ttccaattaa tgaattggat
    27481 aaatttgtgg aagcaggagc ttgtggaaca gcagctgtta tctctccaat tggtggtatc
    27541 cagtacaagg ataacttaca tgtcttttac agtgaaacag aagttggtcc tgtgacacgc
    27601 cgtttatacg atgagttggt cggtattcaa tttggggata ttgaagcacc agaaggttgg
    27661 attgtaaaag tagattaaaa tagagcttgc tcttgcaagc ttttttgatt ttgtgtaaaa
    27721 tagacaaagt gaggtaaagt gatgagtaaa aaagataaaa aaattgaaat ccaactgatt
    27781 gatcacaaag ttatggttaa tgaaactaag attgatggct atcaattaca aattggtaaa
    27841 cgtgtggttg gagaaattgc agaacttgat gagaaatttg ctgtcttaaa aaatgatgga
    27901 gtagattgct tcttcaaaac gctcgaacaa gcggttgaaa atatcattga aaactacaat
    27961 ttgaaccatt aaaattagac aaaaacactt gccagcttac aaaaactatg atagaatagt
    28021 ttttgttgtt acggaaagat agcgaagagg ctaaacgcgg cggactgtaa atccgctcct
    28081 tcgggttcgg gggttcgaat ccctctcttt ccatttcgga aactaatcta attagaaaat
    28141 tagttttctg taaaaaatat ttttggggta tagccaagcg gtaaggcaag ggactttgac
    28201 tccctcatgc gttggttcga atccagctac cccagtcaga ggtatcaggt gctccctgag
    28261 accgtcaaag ataagagttg ttgtcgttgc gggttacctt gagaaaatat attgttatca
    28321 aaagctagca ttggctagct tttgttggag gattttttag aatgaatgaa tttgaagatt
    28381 tgctaaacag tgttagcgaa gtgaaccctg gtgatgttgt cactgcggaa gttttaactg
    28441 ttgataacgg tcaagcaaac gttgttatcg aaggaacagg tgttgaaggt gtcttgacac
    28501 ttcgtgaatt gactaacgac cgcgatgctg atattaacga ctttgttaaa gctggcgaca
    28561 cagttgaagt acttgttctt cgtcaagtag taggtaaaga tactgataca gttactttcc
    28621 tagtatctaa aaaacgtttg gaagctcgta aagcctggga caaacttgtt ggtcgtgaag
    28681 gcgaagttgt tactgttaaa ggtacccgtg ctgttaaagg tggtctttca gttgaatttg
    28741 aaggacttcg tggatttatc cctgcttcaa tgattgacac tcgttttgtt cgcaacactg
    28801 aaaaatttgt tggacaagag tttgatgcta aaatcaaaga agttgatgca gcagaaaacc
    28861 gtttcatcct atcacgtcgt gaagttatcg aagaggccgc taaagaagct cgtgctgaag
    28921 tgttctctaa gatttcagaa ggtgcagtcg ttacaggtac tgtagcgcgt ttaacaagtt
    28981 ttggtgcttt cattgacctt ggtggtgttg acggacttgt tcacgtgact gaattgtctc
    29041 acgaacgtaa tgtgtcacct aaatcagttg tttcagttgg tgaagaagtt gaagttaaag
    29101 ttctttcaat tgacgaagaa gctggtcgtg tgtcactttc acttaaagca acaacacctg
    29161 gaccatggga cggcgttgaa caaaaacttg ctcaaggtga tgttgttgaa ggtaaagtaa
    29221 aacgcttgac tgacttcggt gcttttgttg aagtattacc aggtattgat ggacttgttc
    29281 atatttcaca aatttcacac aaacgtgttg aaaatccaaa agatgtactt tctgtaggac
    29341 aagaagtaac agttaaagtt cttgaagtga atgcagctga tgagcgcgta tcattatcaa
    29401 tcaaagctct tgaagaacgc ccagcgcaag ctgaaggaga caacaaagaa gaaaaacgtc
    29461 aatcacgccc acgtcgtcca aaacgtgaat caagacgtga ctacgaactc ccagaaacac
    29521 aaactggatt ctcaatggct gatcttttcg gtgatattga attgtaataa atctaaaaag
    29581 ttgaggtttt cctcaacttt ttattgtctt ctggttagaa aagttatttc atattatttc
    29641 attttatgat atactataag ggtattgaca aagttgtgac cacctttagt gtaatggata
    29701 tcacgtaaga ttccggttct tgagatgggg gttcgattcc ctcaaggtgg atagaaatca
    29761 tataaaaaag ccctatttga taggcttttt ttgtttcttt tccaaaattt gtccaaaatt
    29821 atctagaaga gttgacgaac cttttcaaaa tcattatttt tctgaccttg aaaacgatga
    29881 gtataggttt taaatatctc tgtaatgcca cagcctgaat tttatttgac tcagattcat
    29941 aagattgtaa gtaatcaagg ttaattattg atgagatggt gaaaccattt ttagcaagtg
    30001 ctattaaagc atttagtgat ttttcgaaac gttttaaaat ttaagaagag ttttacctaa
    30061 aaaactagct gtgtcatctg tctaagcaca gttagttttt tagtattaga tttttaaaga
    30121 agaaacgtct caatcttaat tggataatcc ttatatttgg attttaatat agtagctact
    30181 tcttgatata gtatccgaat ctgcgctctt ttttcaggca tgttaaaacc aacataatgg
    30241 aattgtgatt caatagttac aaataaatca tggaacaggt gattcatagt ttctagttta
    30301 tggcaaagca gtcgatcttc ttgaatgaat gttgggggat tttgatcttc tccccaataa
    30361 gcataaacac gtgacaacgg ataagtccca tattctaaac aaagttcata agtcattttg
    30421 gcctccttgt tttatcttag cattttctaa ataaagcttc taatgatctg cttaaaggag
    30481 taatgggttg catctcatct catcaaaaat aaattgagat ctattttgtg aaaaaaaaga
    30541 caaacaaatg acttgcctag acttgttttt cctagatata ataggaagta actgaataac
    30601 aatgaaggag aaaaaatgtc agaaaaaatc agagttttac tctattataa atatgtttca
    30661 attgaaaatg cgcaagagta cgctgcgaaa catctagaat tttgtaaatc tattgggctt
    30721 aaaggtcgta ttttgattgc ggatgagggg attaatggaa ctgtttctgg agattatgaa
    30781 acaactcaga agtatatgga ttgggtccac agcgatgagc gttttgctga tctttggttc
    30841 aagattgacg aggaaaatca acaagctttc cgtaaaatgt ttgttcgtta taagaaagag
    30901 atcgtgcacc ttgggttaga agacaataac tttgatagtg atattaatcc tttggaaaca
    30961 acaggtgagt atttaaatcc taaacaattt aaagaagcct tgcttgatga agatactgtt
    31021 gttttagata cgcgtaatga ttatgaatat gacttaggtc acttccgtgg agcaattcgt
    31081 cctgatattc gcaatttccg tgagttacca caatgggtac gtgacaacaa agataagttt
    31141 atggaaaaac gtgttgttgt ttactgtacg ggtggtgttc gctgtgagaa attctcgggt
    31201 tggatggttc gagaaggctt taaagatgtt ggacaattac acggtggtat tgcaacatat
    31261 ggaaaagatc cggaagttca aggtgaactt tgggatggtg cgatgtatgt ttttgatgat
    31321 cgtatctcgg tgccaattaa tcatgttaat ccaactgtca ttagtaaaga ttattttgat
    31381 ggaacacctt gtgaacgtta tgtcaattgt gcaaatccgt tttgtaacaa acaaattttt
    31441 gcttcagaag aaaacgaaac aaagtatgtt cgaggatgct cgcctgaatg tcgtgcacat
    31501 gaacgtaatc gctatgtgca agaaaatggt ttatcacgtc aaggatgggc agaacgttta
    31561 gaagctattg gagaatcctt gccagaattt gtaggtgcct aatatctcaa aacaaatcag
    31621 gaaactggtt tgttttttgt gatacagatg atgagaccaa attgcaaaga ctttgcaaca
    31681 agatactagt gaacgtggac accaagctca ttttgtcatg cagagccatc aacagcgaag
    31741 acgtcaacac caactctgtc tagaaccttt tcaaaagaat tgtgagtttc tgtattacga
    31801 gatagagaag gtaaaactgg attctgaaga ctgccttacc atcaatcctt tgggaatggt
    31861 ttaagcttta cagcttgatg atggtcaaat cgtcacacag gcaggtatta tcttgctctt
    31921 tatagcgaaa caaaccctga aatctcagct cattccccaa gataatcttt tggcagaaag
    31981 tcgattcaac gaaatcatgg atttcttaac aggtgatttt ccccttgttt ttaggccaat
    32041 gtacaaccct catcgctaca ctatttctca agacaatcaa gcgcttgaaa aagtcaaaca
    32101 agcttcttac aagcggatgg atattgctat gacgcattta gatggtctta ttggggatag
    32161 tgggcatgtt tatcgagatc aacaaaccat tgcagatgct tatgcttacg ccatggcgct
    32221 atggtctcaa aaaacaccaa aatcttacga gaattatccg catctggcac gccaaacgca
    32281 tgaagaaact tttgttttca gaaaactaga ttcataatga caaaatcaat caaagtagta
    32341 cactatagat gaggtgacta cgatgattga ttttattatt tctattgatg attgcgcagt
    32401 tgaattggat agtcgtcaat cttggaaaat tcgctacccc ttatcaacca ttctatttct
    32461 tgtcttcgtt tgtcagttag ctggcattga aacctggaag gagatggaag attttattga
    32521 aatgaatgaa ccattgtttg cgacctacgt tgatttgagt gaaggttgtc cgtctcatga
    32581 taccttagag cgtgtgatta gtcttgttaa tacagaccgt ttaaaagagc ttaaagttca
    32641 atttgagcaa tcattgacaa gcttagatgc cgttcatcaa ctgatttcag tggacggtaa
    32701 aacgattcga ggcaatcgag gtaaaaatca gaagcctgtt catattgtaa cggcttatga
    32761 tgggggtcat catcttagtt tgggacaggt agcggttgag gagaaaagta atgaaattgt
    32821 tgccattcct cagttattgc ggacaattga tatccgtaaa agcattgtaa cgatagacgc
    32881 aatgggcacg cagacggcta tcgttgatac gattataaaa ggtaaagcag actattgctt
    32941 agccgtcaaa ggaaatcaag aaacacttta tgatgatatt gctctttatt ttagtgatgt
    33001 caacttattg gaagaactcc aagaaaatgc acagtattat cagactgttg aaaaatctag
    33061 gggacagatt gaagttagag aatactgggt gtcttccgat atcaaatggt tgtgtcaaaa
    33121 ccatcccaaa tggcataagt tacg
//
LOCUS       DATUYR010000015        31776 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.015, whole genome shotgun sequence.
ACCESSION   DATUYR010000015 DATUYR010000000
VERSION     DATUYR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31776
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.015"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..742)
                     /locus_tag="VCI33_000645"
     CDS             complement(<1..742)
                     /locus_tag="VCI33_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEP2831583.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQV"
     gene            complement(762..3248)
                     /gene="gyrA"
                     /locus_tag="VCI33_000646"
     CDS             complement(762..3248)
                     /gene="gyrA"
                     /locus_tag="VCI33_000646"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEP2831584.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIAEINTDRENSISKNKDN"
     gene            3439..4422
                     /locus_tag="VCI33_000647"
     CDS             3439..4422
                     /locus_tag="VCI33_000647"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEP2831585.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            complement(4581..5951)
                     /locus_tag="VCI33_000648"
     CDS             complement(4581..5951)
                     /locus_tag="VCI33_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEP2831586.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDTKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDMSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(6192..7919)
                     /locus_tag="VCI33_000649"
     CDS             complement(6192..7919)
                     /locus_tag="VCI33_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831587.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQLVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLIPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEVLRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRGEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            complement(7922..9640)
                     /locus_tag="VCI33_000650"
     CDS             complement(7922..9640)
                     /locus_tag="VCI33_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831588.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLVSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEVPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPINDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGE"
     gene            complement(9650..10249)
                     /locus_tag="VCI33_000651"
     CDS             complement(9650..10249)
                     /locus_tag="VCI33_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEP2831589.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            complement(10250..11227)
                     /locus_tag="VCI33_000652"
     CDS             complement(10250..11227)
                     /locus_tag="VCI33_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEP2831590.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKDVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            complement(11234..12490)
                     /locus_tag="VCI33_000653"
     CDS             complement(11234..12490)
                     /locus_tag="VCI33_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEP2831591.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     HYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIAHLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            complement(12480..12932)
                     /locus_tag="VCI33_000654"
     CDS             complement(12480..12932)
                     /locus_tag="VCI33_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2831592.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            complement(12950..13540)
                     /locus_tag="VCI33_000655"
     CDS             complement(12950..13540)
                     /locus_tag="VCI33_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEP2831593.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITTALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            complement(13524..14363)
                     /gene="prmC"
                     /locus_tag="VCI33_000656"
     CDS             complement(13524..14363)
                     /gene="prmC"
                     /locus_tag="VCI33_000656"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEP2831594.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLSYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            complement(14363..15442)
                     /gene="prfA"
                     /locus_tag="VCI33_000657"
     CDS             complement(14363..15442)
                     /gene="prfA"
                     /locus_tag="VCI33_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEP2831595.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            complement(15477..16046)
                     /locus_tag="VCI33_000658"
     CDS             complement(15477..16046)
                     /locus_tag="VCI33_000658"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEP2831596.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            16184..16369
                     /locus_tag="VCI33_000659"
     CDS             16184..16369
                     /locus_tag="VCI33_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEP2831597.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            16422..17360
                     /locus_tag="VCI33_000660"
     CDS             16422..17360
                     /locus_tag="VCI33_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEP2831598.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPLSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTIMAPSSFYCDIWATRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(17424..18707)
                     /locus_tag="VCI33_000661"
     CDS             complement(17424..18707)
                     /locus_tag="VCI33_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEP2831599.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVVGTLVSAMMGLVDTTPVVEASWIH
                     VLTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(18707..19288)
                     /locus_tag="VCI33_000662"
     CDS             complement(18707..19288)
                     /locus_tag="VCI33_000662"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEP2831600.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     regulatory      complement(19375..19470)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(19593..20576)
                     /gene="guaC"
                     /locus_tag="VCI33_000663"
     CDS             complement(19593..20576)
                     /gene="guaC"
                     /locus_tag="VCI33_000663"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEP2831601.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAERLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKACEY
                     EFVTSLKEDAPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVDGETFKEYYGSASEYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(20854..22380)
                     /locus_tag="VCI33_000664"
     CDS             complement(20854..22380)
                     /locus_tag="VCI33_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEP2831602.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            complement(22373..23101)
                     /locus_tag="VCI33_000665"
     CDS             complement(22373..23101)
                     /locus_tag="VCI33_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831603.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVANLFGGDEHA"
     gene            23524..24213
                     /locus_tag="VCI33_000666"
     CDS             23524..24213
                     /locus_tag="VCI33_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEP2831604.1"
                     /translation="MKNAISILALLTSENQLEDMFIRELSHHFSHLFEVTILTSKANI
                     ESNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTRF
                     YQKFDRHFLQYLPLQARLSDANGNIIVDNHAFNGSFLPTTDKEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            complement(24407..25162)
                     /locus_tag="VCI33_000667"
     CDS             complement(24407..25162)
                     /locus_tag="VCI33_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP2831605.1"
                     /translation="MGNIALVTGASAGFGRAIVEQLIADGIKVIGASRRLEKLKALET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGTHIRVTNIEPGLCDGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(25289..26284)
                     /gene="pta"
                     /locus_tag="VCI33_000668"
     CDS             complement(25289..26284)
                     /gene="pta"
                     /locus_tag="VCI33_000668"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEP2831606.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            complement(26288..27193)
                     /locus_tag="VCI33_000669"
     CDS             complement(26288..27193)
                     /locus_tag="VCI33_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP2831607.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            complement(27190..28026)
                     /locus_tag="VCI33_000670"
     CDS             complement(27190..28026)
                     /locus_tag="VCI33_000670"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEP2831608.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWKRVKDAFIGEI
                     DS"
     gene            complement(28001..28672)
                     /locus_tag="VCI33_000671"
     CDS             complement(28001..28672)
                     /locus_tag="VCI33_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP2831609.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            28760..29338
                     /locus_tag="VCI33_000672"
     CDS             28760..29338
                     /locus_tag="VCI33_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEP2831610.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFANSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHIAFKYAKSKVARCINTLKKFNDK"
     gene            29478..30458
                     /locus_tag="VCI33_000673"
     CDS             29478..30458
                     /locus_tag="VCI33_000673"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP2831611.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAAEVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            30455..31582
                     /locus_tag="VCI33_000674"
     CDS             30455..31582
                     /locus_tag="VCI33_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP2831612.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            31572..>31776
                     /locus_tag="VCI33_000675"
     CDS             31572..>31776
                     /locus_tag="VCI33_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP2831613.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLEKIPNSGDGFPLKITINKELTG"
BASE COUNT         9813 a         6580 c         5521 g         9862 t
ORIGIN      
        1 atacttggtt ataagaatga ttaaaagctt tcaatacatc ggcgggcgct ttatcaaaat
       61 cgtattctgt ttttagttct cctttgacaa taatacgttc tgttgcttcg atatctgtac
      121 aagtcactaa agtcacttct ttgagaccag ctgtatcatc gataacatca acgcgttcag
      181 gagctaccgt gaaaacatct tttatgatgt attcgtaaat tttttcttta tctgttaaat
      241 agatggacat cccattttgt gctctttcaa gcggcgaaaa gagcatttga gatgaacctg
      301 taattccaaa aatatgatga ctggcaagag aataattatt ttctcctccc ataacttgtt
      361 cttctttcat cgttcctgcg ccataaataa gctcagtatt tcctaaacct ttaaaaattg
      421 gtaaattaat gccaagctct ggtatggcaa tgccccctat aacaggaagt tgctgagccg
      481 ccatttgtgc ttgcaagaca ctctctgtac taactggctc tacggcttgg aaatcaaatg
      541 ttgacttagc ctctttattc tttttgattt gttttttcga aactttcgtt acttgatatt
      601 tattagagtt tcgagcaatt aatgtatttc ggataggttt gttaaaaagc aatgctaaac
      661 ctagaattaa aagtacagct attagtagtt ttcttgccca agacatggat tttatttttc
      721 gacgcttttg tttttttacc attttttact ccttgttatg attaattatc tttatttttt
      781 gaaatactat tttcacgatc agtattgatt tcagcgatac tactatcttc aggctgaacc
      841 aaggtaaatg ttacaatctt agcatcagca tctagtttca taattttaac acccaaagta
      901 gcacgacctg tttgcgaaat atttgcaaca tttgtgcgaa taatgacacc tttatttgta
      961 atcaccatga tatcttctgt accatctacg gtaaccaatc cagctaattg accattttta
     1021 ggggtaatat tagctgtttt tattcctttt ccccccctac cttttgtagg atagtctgta
     1081 gcacttgttc tcttaccaaa gccattttcg gtaattacaa gaacttcttg gttatcttga
     1141 atacgtgacg cgccaacaac gcgatcatct tcacgaagct taaccccgcg aacacccgta
     1201 gctgatcggc ccatgttgcg gatgcttgct tcattaaaac gaacagaata tccggagtga
     1261 gttccaatga taatatcatc ctgaccgctt gtcaaaagga cgttaatcag ctgatctccc
     1321 tctttcaact taagagcacg gagaccgttt tgacggatat tattaaattc tgaaacttct
     1381 gtacgtttga caatgccttg tttcgttgta aagaagaagg atttacctgc tgtttcttcc
     1441 ttacgtgcgt tgataatagt ttgaatggtt tcaccatctt ctaatttcaa tagattaaca
     1501 attggaagac cttttgctgt acgaccatat tctggaattt cgtaagcttt caaccgatag
     1561 acacgcccaa aattcgtaaa gaagagcaaa gtatcatggg tacttgttga taccaattca
     1621 cggacaaagt cgtcattgtt gactccagtt ccttggacgc cacgtccacc acgcttctgg
     1681 gcacgaaatt catcttgagc aagacgcttg atataacctt tatttgacaa ggtaatcaaa
     1741 acatcttctt cttcaattag gtcttcatct tctagagaaa ggacttcacc gaccattaat
     1801 tctgtacggc gtgggttagc gtatttacgc ttgatctcat ccatttcttc tttgatgata
     1861 gtgataatac gttctggttt ggctaaaata tcagataagt cagcaatcaa ggccaacaaa
     1921 tcatcatatt ctgattgaat cttatcacgt tccaaaccag tcaaacgacg caaacgcata
     1981 tccaaaatag cttggctttg gcgttcagac aagtcgaaac gtgacatcaa ttctgtctga
     2041 gcaatgacat ctgtctcact gttacgaata atagcaatca cttcgtctaa atgatcaagg
     2101 gcaatcagta agccttctaa aatatgagct ctggcttcag ctttatcttt atcaaatcgg
     2161 gtgcgacgta taataacttc tttttgatga ctaatgtaat tatcaatgat ttgtctgagt
     2221 gacaatattt taggcacacc attttcaata gccaacatat taaaactgaa attcgtctga
     2281 aggcttgtta atttaaagag gttatttaag ataactgtcg cggatgcctc tcgacgaatc
     2341 tcgataacaa aacgcactcc ttcacggcta gattcgtcac gaactgctgt aataccttct
     2401 agtctttttt cttgtgctaa acgaacaatg tgttcatgca ctttcgtttt attgacgcca
     2461 tagggaaatt cagtaaccac aatacgttcc cgacctgttt gagttgtttc aatttctgtt
     2521 cgagagcgta aaacgataga acctttacca gtatcataag ccctatgaat acctgagcgc
     2581 cccataacaa gggcaccagt cggaaagtca ggtccaggga tcacttccat caactctctt
     2641 gttgtacagt ctggatgttc cattaccatc ttgacagcat caatagactc tgccaaatta
     2701 tgaggcggaa tgttagttgc catcccaacg gcaatccctg tcgctccatt gactaataaa
     2761 ttaggaaagc gagctggtaa tactactggt tctctttcgc ttccatcgta gttatcttga
     2821 aaattaacgg tatttttatt gatatccctt aataattcaa gcgcaatttt actcatgcgc
     2881 gcttcagtat aacgttgagc ggcagcacca tcaccatcca tagaaccaaa attgccatgg
     2941 ccatcaacaa gcatatggcg ataactccac cactgagcca tacgaaccat agcttcataa
     3001 atagaagaat ccccatgcgg gtgatattta cccatgacat cacccgtaat acgagcagat
     3061 tttttatgcg gtttatcagg tgtcacaccg agttcattca tcccatagag aatacgacga
     3121 tgcacaggtt tcagaccatc acgcacatct ggcaaagctc ttgccacaat gacactcatg
     3181 gcatagtcaa tgaaactcgt tttcatttca cttgtcagat tgacgtcaat taaatttcga
     3241 tcttgcattt aaggaatgct ccttttctag tcaatactag atttcattat accataaatt
     3301 ccttattttt tcttattgga agcgttatca gtaaactatt ttcattattt ttctgaattc
     3361 aatgacaaac aattatcaat atgttaaaat aagaccgtat gagcttttgc tctaataaaa
     3421 ttaaggagat gtttagaaat gactgcaact aaacaacaca aaaaagtaat cttggtcggt
     3481 gatggtgccg tcggttcatc atacgctttt gcattggtaa cacaaaatat tgctcaagaa
     3541 cttggtatta ttgatatttt taaagaaaaa actcaaggag atgcagaaga tttaagccac
     3601 gctctagctt ttacatcacc taaaaaaatc tatgcagctg actactcaga ctgtcatgat
     3661 gctgatcttg ttgtcttaac agctggtgct cctcaaaaac caggtgaaac acgccttgac
     3721 ctggttgaaa aaaaccttcg tatcaataaa gaggttgtta cacaaatcgt tgcatctggt
     3781 ttcaaaggaa tctttcttgt tgctgctaac ccagttgacg ttttgactta ctcaacatgg
     3841 aaattctcag gtttccctaa agagcgtgtc attggttcag gtacatctct agattcagcc
     3901 cgtttccgtc aagctctggc tgctaaaatc ggtgttgatg cgcgttcggt tcacgcttac
     3961 atcatgggag aacacggtga ctctgaattt gctgtttggt cacacgctaa cgttgctggt
     4021 gtaggtcttt acgactggct ccaagctaat cgtgacattg atgagcaagg cctagttgat
     4081 cttttcattt ctgtacgtga tgctgcttac tcaatcatca ataaaaaagg tgctactttc
     4141 tacggtattg cagttgccct agctcgtatt acaaaagcta ttcttgatga tgaaaatgct
     4201 gttcttccgc tttctgtttt ccaagaaggc caatacgaag gtgttgaaga ttgctatatt
     4261 ggtcaaccag ctatcgttgg agcgtatgga attgttcgtc cagtaaacat tccacttaac
     4321 gatgctgaat tgcaaaaaat gcaagcctct gcaaatcaat tgaaagctat cattgatgaa
     4381 gcctttgcta aggaagaatt tgcttctgct gctaaaaact aatcacaaaa gagacttcgg
     4441 tctctttttg ttttgcctga tattcctttt ccaacgtgct ataagaggat aatcataagt
     4501 ttagaacttt tcaggttgca acaattaaaa agcaaaaaag ccagtgctgc tgaactgctc
     4561 tggctttttc ctatcagata ctagtctttg gcaccaagtg ctgccattgt gatgtagttg
     4621 taaggtttgt tgaaatgtgg taagaagaaa atatcagtta atgccaactt ttcaatggtc
     4681 actccttctt ggatagcaag tgaaaacatg tgaattccca ttgacatatc ttcacgggct
     4741 gccatttgag cacccaagat acgacgtgag tctttatcgt aaacaatctt aatggttact
     4801 gggaagttac cgtgttcgat aaattcaggt ttttgattat cagtatactc agtcactgca
     4861 gcatcaaaac caagtcgttt agctttttca agagtcaaac cagttgatac catgtgcaag
     4921 ccgtaaatgg aaataccgtt tgaaccttga acgccaatac cttcaaggtc tgtaccacaa
     4981 gcgttatgtg ctgctacaat tcctgtacgg acagcatttg aagctaaagc aatgtagttt
     5041 gtatcgcgag tagcattatc gtagatagtt gcacagtcac caatagcata aacacctgga
     5101 atagaagttt cttggcgttt attgacaagg aaagcaccat tacggaaaag atcaatctta
     5161 ccatttccaa gagttgtgtt tggacggaaa ccaacggcga ggataaccat atccacatcg
     5221 tactcatttt tgtcagtaat gatcttttca accttaccat taccagctac ttctttaact
     5281 gtttcaccaa aggctagttg aataccatgt tcttccatgt ttttagccat taagtcagtc
     5341 aaatcacggt cgtaataacc tgccaagcaa gtgtccacga catcaatcag aaccacttct
     5401 ttgcctttgc gttggaaagc ttcagctaac tcaacaccaa tgtaaccagc accaactacg
     5461 gctacacgtt taatgtcttt gttttcaagc tttgcaatca catcagctga gttttggtat
     5521 aacttcacaa attgaagatt ttcaagagtt gcttcaaatt caagtgatcc ttctttgatt
     5581 tcggcacctt taatcggtgg taagataggt tgtgagccag ttgcaaaaat caacttgtcg
     5641 taagtttcca cgtggttttt accatcaaca agcgctgtca ctgttttggt atcgtagtcg
     5701 attgattgaa caggtgattc catgtaaact ttagccccta aagactctaa ttcttcttta
     5761 tctgaataga aaagtccttc tggtccagca atttgctcac caatccaaag tgccatacca
     5821 cagcctaaaa atgaaatatt tgagttttgg tcaaatacaa caatctcatt agcatcaccg
     5881 tagtttgtta acatagtctt gatacaggcc gtaccagcat ggtttgcacc aacaacaacg
     5941 attttactca tatattttgt tcctcatttc acaatattat atagggctct attataccac
     6001 agtcaaaaaa cgcttccaaa tatattgctc acttttttga gttatttctt ctttagtcaa
     6061 ctgttttttc tcacttataa aaaaagatcg tccgcttttt cgctgttatt tttttatata
     6121 acagtttaaa gtttgttaat taactaactt taggaagact taaaaagttc acctttgtta
     6181 aaagacgaac tttattctat cttttgtaat ctagcataac atccgttttt tgacattaag
     6241 ctggcatgtg ttccccactc aaccaatcga ccttgatcca taacaagaat caaatcagct
     6301 gattgaatcg ttgataaacg atgggcaatg ataaagctgg tccttcctct catgagttct
     6361 tttagtgcct cttgaataac agcttctgtt ctaatatcaa tagaggaggt ggcttcatct
     6421 agaatcaaga cttttggttt tttcaaaaac attctggcaa tagctaataa ctggagctgc
     6481 ccttgggaca aagcatcatc agaagcactt aagtaagtat cataagtctt aggaagttgc
     6541 ataataaaga agtgtgcatg agctgctttg gctgctgcca ctacttcacc acggctagcc
     6601 tcttcactgc cgtaagcaat caactcatga acagtcgcat ctttcaacca ggtttcttgc
     6661 aataccatgc cagtaatgga cctaagttct tccttagcat aacacttaat aggaacttta
     6721 tctaacttga tgctaccagc atcaagttca taaaagcgca tgagtaaatt gattaaagtc
     6781 gatttgcctg cacctgtagg ccctacaatc gctaccttgg aacccgcagg aacagaaaaa
     6841 ttaatatcct tcagtaaggg cctgtctttt tgataagaaa aacttacttc ttcaaagtca
     6901 atttgtccct taaccgctga tgccttaaat gttagaaagt gctcttgctc ttttatttca
     6961 atatctaaaa ggtcataaag cctttgcgca caagctaaag aactctgtat ctctgccaag
     7021 acagatgaaa tatcgttgaa aggtttggta tattggacaa caacatttaa aaaagtggtt
     7081 agctgaccta ctgaaaaaag tccagccatg atacgaacag cccctaaacc tgccagtaaa
     7141 gcataaatga cactattaat aaagcgagtg gctggattga ctgttgaggc gtaaaaaatg
     7201 gctttttgtg aagcctcgca ataggtttta tttaaaacat gataatcaca gatagactgc
     7261 tcttgagcat taaacagttg aaccaaacct tcttgacgaa ggacttcttc agtaaactga
     7321 gctaaatttc ctctgctttt ggtctgtgct tgtgcataat gaaaactctt ttgagcaata
     7381 aaacgagcta aaaagaggga gcttggtatt aacaccaaaa ccaagattaa catgagccag
     7441 tcaatctgtg ccatagctat aatggtacac agaatcgtca atagtccaag tataaactgg
     7501 ttaaaaacca attgtagacc attgatcagt tgctcagtgt cggttatcac tcgactaacc
     7561 aaatctccaa tggtttgacg atctagatat gcaaaaggaa gacgatgaat tttttccaaa
     7621 agcttatctt tcaattgctg gctatattga tatagcaaac gactatagac aagaggcatc
     7681 acccattgaa tcaatgtgtt gaccacaata acaactaaca tttgccccaa cagccatttt
     7741 aaagtttgcc aagaattgac tgacaaactc atatcaacgg ctttaccgat aagaacaggc
     7801 aagtaaacac ttaaaccaac ttgtacaaaa ctagctatca cgagaataca aacaggtaga
     7861 ggttttttta acaaatcctg aagcactcgc tttaggaggt gatggtctgt tttaatcatt
     7921 tttactcccc cttcacgtaa agcgctagca tttcttgata aagtgcattg cttactttta
     7981 actggtcatg actggcaaaa tccaattgat ggccctgttc caacactaaa atcttatcag
     8041 caaactgcaa attcttaatg gattgcgtga ctagaatcac taaaacatct gataactctt
     8101 cttttagagc tttaaaaagt cgtcctcgag tctcattgtc taaggcggaa ctggcatcat
     8161 ccaaaatcaa aatgggtttt ggcttcagaa gtgctctggc aatagctaga cgttgccgtt
     8221 gccctcctga aaaatgacgg ccaaaggctt caacaggtgc ctctaattgt tctggtaagg
     8281 cagcgacaaa ctcttttgct tgagctaatt caagagcccg ccacagctcc tcatcattaa
     8341 taggcattga ttgtcccagt aggaggttac ttctaatcgt tcctttaaat aattgagctt
     8401 tttgaggaac aagagcaatc acatttcgcc actcggttag tgtttttggc acttctcctt
     8461 gccaattaag actaatctca ccactatatt gtgaataagt ttggcagatc aagtcaatca
     8521 aagtcgtttt gcctgctcca gtaccaccaa tgatgccaat ccattctccc tgatcagcac
     8581 ttaactgaat atcatacaga cttggctctg ctgctcctgg ataagtaaag gtcaagtgcc
     8641 ggatggtcaa cagatgagtt gaaataggac catctttaag taagggcaaa ggcacttcat
     8701 cagctaaaac aaacacctga ttaatccgtt ttgcggctgt tacactttga ttgatggttc
     8761 ccatcaacat ggtcattttc aaaagttctg ccaaaatctg caagaggtaa ttaattaaag
     8821 caaccaacat gccctgacta agcgatctat gagctacttg ccaacttcct tgccaaatca
     8881 agattaacag agtaagattg accactaaaa aagtcatagg attcaagaca ttggctaggg
     8941 tagcagcttg atattggtgg cgacttaaga gcatattttg ctgcttaaat gcttggagtt
     9001 ctttttgggt ttgattaaaa gccttaatta ctcggattcc ttgtaactgt tggctagtca
     9061 aacgaaccaa gtgatctgtt tctcggcgga ttaagagata aaggggacct aataaatgtg
     9121 acataacaaa aacaagtgtt agtaagacaa tcaccatcat cacaaaccat agggttagac
     9181 ttggactaat ccaataagcc attaccatag caccacatac gataataggg gcccttagaa
     9241 aaagacgcaa aaattgattg ataccagttt gaatttgaaa actatcactg gtcaatcgtg
     9301 ataccagact agcataacca agatggtctt ggtcctcctt gctcaaaaac atgatttttt
     9361 taaacaaatc atctgtcatt tgtcttgtaa aaccaacagc tgctttcgaa gaaaaatact
     9421 gggctgtaac ggaaagcaat aagccaatgg ttgctaaacc aaataatgtt aaaacaaccc
     9481 tcaaaattcc ctggctgttg tgttgactaa tcgatatatc aatcatatta gcaattaaca
     9541 aagggactaa taattcaaat aaagcttcta ataatttaaa aagaggacct aagatacttt
     9601 cttttaggta ccctttcaaa tatggacgta atcgtttcat gataactttc taaaaaaggc
     9661 tcatcagttc aattaaataa agattgaaat gaacttctcg tgagtaatag atattgccgc
     9721 cattcttata aagcttacct ccagaaatca aggcgagggg gtggtaataa aaataggttt
     9781 gaccactagt gttgcctagg cttggcgcca caacggtttt ggaataggta gtcgctaaat
     9841 caacggtatt ttgacctcca tgtttagcaa tataatcaat ataagctgtt ccaaaattat
     9901 aggcctgaac agccgtccaa gaatctactc ctgcttcctc agctagagca agattatgag
     9961 acagtaaatt caccccgtgc tcaatactgg cctgactatc tgtaatcgag tttttttgtc
    10021 ctgaactgct ttcactggat tgcatgacat ctgcttctcc tccttttgtc tcggtgtata
    10081 tcatagctaa gactaagtct acatttgcct ttgtatcatt ttctgctaat gttttctcaa
    10141 ccataggctt atatgccaaa acacgttgaa cattgtgatg aataacaaag gattgataaa
    10201 tgacaaaaag aagaagaaaa ctgcaggcac gctttaaaag tctaaacatc tatttggttt
    10261 taatgtcctc tatattttta atcaagattg aataagtccc atcatcattt aacaaaaatt
    10321 caaccgcttc agcatcctga taaatggcat ttggtactgt taattctatc ccgtttgata
    10381 acgatagctt ttgattttct agttttttta tctgtctaga atgatcaata tcgctaattt
    10441 ttataggttc tggaatgaca tctttgactt gatccacaaa ggttaaacgg gctgttaaat
    10501 tatcatcaaa gagttggtca gctaattttt ctggtgataa aacgtcgtct gcttctaact
    10561 gcttaaaaag agtggatttc atctttgact gaaaagtaaa atcatcttga ttaaaatgct
    10621 ctgcaatttt ttgagcggtt tgttctatca ttttgataga ctttttaaca gattgctcag
    10681 gtgtaacttt caagaggtgt tctgaaaaat aattagcaaa actgccatta tgcttcacac
    10741 gcttttcaat caagtagtac tgaccagatt tgagattaat gactagtgct tcatctggtg
    10801 tctgggtggg actcggcaga ttattctgcg tcaccgtaaa tggatgttca taattgtcag
    10861 aaaggtgagc aaactgttct ttcaaagcaa ttcgcaagaa agcaaaaaag ggttcgccat
    10921 ccttatcaaa ctggacaaag actaaatcat tggttttttg atcctctgaa ataacaaagg
    10981 cttccttcca taactgggca atggtaacag acgtttccaa caaatcatca ccaatggttg
    11041 taaaaaaggt attattagcc tcaaactgac cccgtttggc ttcatccgag aacactttag
    11101 ccaatttttt ccggaaatat tcatcaattc gtggtgtaat gctcactaaa cgatctgata
    11161 acagtaactc agtgtcattg ggagaaaatt gatgaatgac aatacgtttt atataactat
    11221 ctaacatgat tccttacttt ttatagagtg ggaaggcatc ggttaactga cgcacttcct
    11281 gacgaacctc ttcaagaata gtttcttgat catgattaac caaggctttg atgatgaggt
    11341 gagcgatagt ttggctttct ttaactccca tgccacgact ggttatagct gcacaaccaa
    11401 tacgaatgcc tgatgtttta aaaggagata aggtttcaaa aggaatggca tttttattta
    11461 gagtaatatt aacctcatcc agaagatttt gcgctagttt accattagca ataaccttag
    11521 tgacatctac taaaaagaca tgattatctg taccacctga gatcagacgg aaacgatcat
    11581 cttgtgcaaa aactgctgcc atagccgcag tattatcaat aatagcttgt gcatagtctt
    11641 taaaggctgg atccaaggct tctttgaagg caaccgcttt ggcagcaatc acgtgttcca
    11701 gaggtcctcc ttgtaaacca gggaaaacgg ccgaattaat ctttttagca agagcttcat
    11761 cattggttaa tatcaagcct ccacgaggac cacgtaaggt tttatgggtt gttgatgtca
    11821 caatatgcgc ataatggaca ggactcgggt gcacacctgc tgctacaagg ccagcgatgt
    11881 gagccatgtc caccattaag taagcaccta cgtgatcagc aatggcacga aacttttcaa
    11941 aatcaatgct tctagagtag gccgaagccc ctgcaacgat aagctttggc tggaccgcct
    12001 ttgcttgttc taaaatcgcc tcataattta acatttcagt atcagcatct actgagtaac
    12061 ccacaaaatg gtaggttttt ccagaaaaat ttactggaga gccatgcgtg agatgaccac
    12121 ctgcagccaa atccattcct aatacggtgt caccagcctc aatcaaggcc atataagctg
    12181 ctgcattggc ttgacttccg gaatgggctt gaacattggc aaatgcggca ccgaaaagtt
    12241 tcttagcacg ttcaatagca agcgtttcga caatatccac acattctgtt ccaccataat
    12301 agcgattacc tggatagcct tctgcatact tattggttaa aacagaacct tgagctgcca
    12361 tgacagcttt tgaaaccatg ttttcagatg caatcaattc gatatgatgt tcttgtcttt
    12421 cttcttcagc atgaatggca tcccaaagct ctttatcaaa gtcttcaaca ttacctttat
    12481 caaaaatcat tgtcatctcc ttgtaagttt ttgatgaatc gtaattgtgt tttatcatct
    12541 gcatagtcga ggtttcggta aaaagcatgc gcttctttac gatgactggc agaattgagg
    12601 cgaataaagg caatgccttc ctgcctagct tgggattcca gggcccttag taaggcactc
    12661 ccaattcccc ttctctgata ggctggtaaa acagccaagc ccaagagatt aagaccatca
    12721 gaagcataaa gggtctcata ccgttcagcg tgaacatagc ctaataattg attggtatcc
    12781 ttgtcggcat aagctaaaaa atagtgatgg caatcctcaa tcaatcgttc atattgcctt
    12841 tctaacaagt caagcgaaac taaatagcca agggctttgg cgttaatttc ttgtagcata
    12901 ggacagtccg ttttacttaa tggtctcaac attccaagcg gctcctctct taaaagcgaa
    12961 gttcaggcac gttggctaat aattcttctt tggtgatagc accttgacgt aaaatcacag
    13021 cacgctcacc tgataagtct aagatggttg aatccttacc tgttagaaaa ggatcatctg
    13081 cataaccaaa cacttgaaag tcaaaatctt gcataatatg atcaaaaact cgaccactag
    13141 ctttgccaga taagtttgca gaaggaccaa ttaaagggcc tgttttttga attaaagctg
    13201 tggtaattgg atgactgggt aagcgaaaac caaccgttga aagacccgaa ttaatccaat
    13261 aaggaacctg gtcattagct tttaaaataa tcgttaacgg acctggtaaa aaggcttggt
    13321 ataatttctt gaggtatctc ggctgttctt ttgaaaaggc taagatacta ttaaaatcag
    13381 ccacattaag gttcattgct ttatctcggg gtctttgttt taagtcataa acagcattta
    13441 ctgctttttc atctaaagcc ttggcaaaaa gaccataaac cgtttctgtt ggcaaaacaa
    13501 gggcatcacc tgattcaatg atactagcta gatactccat aatcaacaac caccattctt
    13561 tctttgccaa atatatccgt tacgactcgt atatgtcttt gaggaaaata ggcttgtaac
    13621 atatctttta taccttcagc ttgcttataa ccaatttcaa agtaaagctt cccctcctta
    13681 gttaagtaat tgtcagcttg ttcaataatc ttacggtaaa tagcatagcc attttcttta
    13741 gcaaaaagtg ccaaatgagg ctctgattgc aaaacattta gactgacttc ttccttatcc
    13801 tcgtaggata tgtagggagg atttgacaca ataatatcaa atgttcctga aatgagacta
    13861 aaaacatctg attcaataaa cgttatatca agctggtaag catcagcatt ggcttttgcc
    13921 aagtcaagag cagctctaga aatatcagat gccgttactt gccagttagg cctttctttt
    13981 ttgagagaaa tagcaatcgc tccactacct gtaccaatat ctaaaacatt caacggtgca
    14041 tcaagatttt cagctaaaat catgtcaact aattcttctg tttctggcct tggaattaat
    14101 acacgcttgt cgactgcaag cttcaaatca cgaaaataag cattacctgt aatatattga
    14161 ggagataaat gctgacttag agaacagaaa atatgttcca aaagaacagc atcttcagga
    14221 gtcacagctt gattttggtg aatcaacata tctagacttg accattcttt gatttctcta
    14281 aaaacatagg agagattttc gcgatcctca tctatttgtt ctagtttatc ttcataagtt
    14341 ctgattaatg tagcatagtt cattagttat tgagagattc taatttcttt gtctgatcat
    14401 acataacgag tgcatcaata acttcatcca tttttccaga taaaatagta tctaattttt
    14461 gaagtgtcaa accaatacga tgatctgtta cacgattttg tgggaaatta taagtacgaa
    14521 tacgttctga acgatcacca gtaccaactg ttgatttacg ttcagcatct tgttcatctt
    14581 gagcaatttg cgcaaaatgg tcagcaacac gagcacgaat gattttcatc gctttatcac
    14641 ggtttttttg ctgagtacgc tcttcttgca tttcaacttt gattcctgta ggaatgtgta
    14701 ccatacgaac tgcagtagca actttattga cattctgacc accagcacca gacgcgtggt
    14761 aaatatcgac gcgaaggtct ttaggatcaa tgtcgtattc tacttcttca acttctggca
    14821 ttaccaaaac agttgcagtt gaagtatgaa cacgaccctg actctctgtc actgggacac
    14881 gttgaacacg atgagctcct gattcatatt taagcttaga ataaactgac tgaccagaaa
    14941 ccatggcaac cacttcttta ataccaccaa caccattaac ggatgattcc ataacttcaa
    15001 aacgccatcc ttgagtttca gcatattttt gatacattgt taaaagatca ccagcaaaaa
    15061 gcgctgcttc atcgccacca gcagcaccac gaatttctaa aataatgttt ttatcatcgt
    15121 taggatcttt aggtaataga aggattttta atttttcttc atactcttct ttagctgctt
    15181 ttgattcttt aagttcttct ttagccattt cttctaattc aggatcacca ctggcatctt
    15241 tgatcatttc ttcagcatct gaaatggttt gaatgacttg cttatattct ctataagctg
    15301 tgacagtttc tcgagtatta gcttcttcac gtgataattc cataaatcgc tttgtatctg
    15361 aaacaacatc tggatcacta agtaactcac ctaactcttc ataacgatct tcaactgcct
    15421 gcaattgatc ataaatattc atttttcttt gtatctcctt taaaaccgat taccttttaa
    15481 atctctggat taaaataatg ttttcgacaa actgggatat atgtttcatt accaccaatt
    15541 tgtatctgat caccttcata gacaggtttt ccgttttctg tccttaaaac catggttgct
    15601 ttcttactac agtattggca aattgttttg atttcatcaa tcttatcggc caataatagc
    15661 aaatgttttg aaccttcaaa aagattgttt tggaaatcat ttttcaaacc aaaagccatg
    15721 acaggaacat ttagctcatc caccacacgc gccaaatcat aaacattttg cttgcttaaa
    15781 aattggcttt cgtcaatcag gacacaataa ggtttttcct ccaattgggc aataaatgtg
    15841 aaaatatcca tgtcatttga aatggggata gcttctcgtc gcataccaat acggcttgag
    15901 acgatgccaa aaccatcacg tgtatcaaga gcacttgtca taatgacaac aggtttgcct
    15961 tgttcttcat agttatgagc aacttttaaa atttcaatgg ttttaccaga attcattgtc
    16021 ccatatttat aatagagttg agccaaagcg gaaaatccta ctttctatag tattctacct
    16081 attttaccac aaaggctgaa gttttcccag ccattgaaaa cagtgtatac tttttcacag
    16141 cattatgtta caatgatgcc caataacata aaggagtaac attatgccat ttgtaacaat
    16201 tgacttgttt gagggacgct cacaagaaca aaaaaatcaa ttagctcgcg aggttaccga
    16261 agtagtctca cgcattgcaa aagcacctaa agaaaatatc catgttttta tcaacgatat
    16321 gcctgaagga acttactatc ctcaaggtga aatgaaacaa aaatcataat gaccttcccc
    16381 cactccttag tgggggtatt ctgctaaaaa gaggactaat tatgtttgtt acgcaacaat
    16441 taaagctaat gggaacagtt attgatattc agatcgaatc tgacaaagct tgccaacaac
    16501 taagtagagt cattgactta ttatatactt acaaaaatcg tttttcagct aatgattcaa
    16561 actctgaatt gatggctatt aatcaggcgg ctggtgttaa acccctcagc gttcactctg
    16621 atttatttaa cctaatccaa attggtaaag ctcatagctt gtcaacacca agtaatctta
    16681 atatcgccat tgggccactt gttcaagcct ggcgaattgg atttgaagat gctagagtgc
    16741 ctagtcataa ccttatctca caacagttag ctctaacaga tcctagacag gttcttatag
    16801 atgacaaaaa acagactgtc tttttacaac aagtaggaat ggctttagac ttaggagcct
    16861 tagcaaaagg ttatattact gataagatta tggcctacct gattgaagat ggtattgact
    16921 ctgcccttat taatttaggt ggaaatgtac gcgttcatgg acctaatccg aaaagccctg
    16981 acaagacttt tcgaattggc attcaaaaac cagatgctaa acgtgggcaa catcttggtg
    17041 tcatcaaggt taacaatcac tctgttgtga cttctggaat ttatgaaagg cagtttacag
    17101 ccaaagggaa acagtatcat catatcttag atcgtcagac aggctatcct atagaaacag
    17161 atatgcttag cttaacgatc atggctcctt cttcttttta ttgtgacata tgggctaccc
    17221 gtttatttgg tcttgatagc tcaatgatta tcacactttt aaataccttt gataatattg
    17281 aaggtcttct agtaacaaga aagcaccatg tccttatgtc aaatggtttg agacactatt
    17341 ttcaaccata ctatcattaa aaggggttaa cggtttgctc aaaactgatc acagaaaaaa
    17401 cggctcaatt agccgttttt taattattca tcttgtttgt ctttgccatt taaaactaaa
    17461 tttaagacaa cagatgtcaa agtggcaatc acaataccat tcgttaagaa catttgagct
    17521 gtctctggta agctggcaaa aagattagtg ccattaaagc ctaaacctgc tgagatggaa
    17581 acggcagcaa taataaaatt atactcgttc ttttggaaat caacacgatt aagcatttgc
    17641 attccttgaa gagcaaccat accaaagaga acaagcatag ctccaccaag aactgggctt
    17701 ggaatcattt gtgccattgc tccaaattta gggagtaggc caatgaccac aaggatacct
    17761 gctgcgtagt aaatcggacg acgggtttta atacctgaga tttggacaag tccaacgttt
    17821 tgagaaaaac cagtgtaagg gaaggtatta aataacccac cgagaaagac tgcgatccct
    17881 tctgagcgat aaccattgcg caaacgtttt tcatctaatt gatcattagt caaatctgaa
    17941 agcgctaagt agacacctgt tgattcaacc atagaaaccg ttgcgatgat acacatcatc
    18001 acaatagaag tgatttcaaa agttggcatg ccaaaataaa atggggttag cacatgaatc
    18061 caagaagcct ctacaacagg agttgtatct actaatccca tcatggctga aaccaaagtt
    18121 cccactacaa gcccaatcaa aatggaaatg gatttcacaa aacctttggt aaatttttgg
    18181 actaaaagaa taatgacaat cgtcaacaat gataacatca tgctttgtgc cgtaggctct
    18241 ttgacgttat ctcccatatt ccccatagca acacctacga gacttaagcc aataacggta
    18301 atgaccgatc ctgtaacaat tggtgggaag aaacgagcaa tcttagaaaa aatacctgcc
    18361 actaaaatga cataaattcc tgacgcaatg agggctccaa acatggcacc tgaaccttgt
    18421 tgcgctccaa taatggatag aggtgccact gattgaaaag cacatcctaa aacaactggt
    18481 aatcccactc ccgtatgctt ggttaacttt aactgtaaga aagtagccac accacacatg
    18541 aaaatatctg tcgaaatcag gtaggtcaac tctctagcgg aataacctaa agcacctgcg
    18601 atcataattg gtactaaaat agatccggca tacatcgaaa gcacatgctg taagccaagg
    18661 acagctgatt gcgagtgaga atgttcctgc tttgttgaat gtgtcattat gcgtcagcct
    18721 ccaagaagtt tagattacca ttttcaaagt ttttaatacg tgctaatgag gtcacttcaa
    18781 tgcccatatc ttcaatcaat cgacgaccat cttggaaaga tttctcaatc acaataccaa
    18841 cgccgacgac ttgtgcccct gcttgaccaa taatctcgat caagcctttg gctgcctgac
    18901 cattagctaa aaagtcatca ataatcaaaa ccttgtcttc tttagatagg aatttaccag
    18961 cgatagacac cgtgcttgtc acttgtttag tgaaagaata aacttctgct gtcaaaatgc
    19021 cttctgtcat ggtaatgttt ttatgttttt tcgcaaaaat cataggaaca tccattgctt
    19081 ctgcagcgta tacggctggt gcaatacctg aagcttcgat tgtaaccact tttgtaatgc
    19141 cagcctcagc atatttttga gcaaacactt taccaattgc tttcatcaac cggtaatcaa
    19201 cttgatgagt taaaaaatta tctaccttta gaatattctc tcctagaata ttgccatctg
    19261 ttaagatgcg ttcttcaagt aactgcataa atctcctttt gattcaaata gaaaaagcct
    19321 gccgcaaagt gagaaactat aagagtttct cactttgcaa ggttaaaaac caagcaagcc
    19381 taaaagctta cttattgtta gacattaagg tgtcttgtag agacgctgcg ccaattcaca
    19441 gcataaataa gtatgtcatt attgaattca ttatgataat ttactatttt accatgtttc
    19501 ttattttttt caatacctac agagatatga taaaagcatc tccagcatta agctaaaaaa
    19561 ttctttactg gtcctagcta actccatatc atttaaatgg aatcaccatt ccaaatggaa
    19621 tttttgacaa taacataatc aacatgacgc aaactgtcta aatctttacc acccgcgtaa
    19681 gaaatggacg attgcaaatc ctgttgcatt tcggtcaggg tatctgataa gtgacctttt
    19741 gtaggcaata agattttttt gccttctaca tttttatgct cgcctttttg atattctgac
    19801 gccgagccat aatattcttt aaacgtttca ccatccactt ctaccgtttt tccaggactt
    19861 tcaatatgtc ctgcaaaaag cgatccaatc atgaccatac tagctccaaa gcggatggat
    19921 ttagcaatat caccatgtgt gcgaatccca ccatcagcaa tgataggttt acgagctgcc
    19981 tttgcgcacc agcgcagtgc tgctagctgc caaccacctg taccgaaacc ggttttaact
    20041 ttagtgatac atactttgcc tggaccgatg cctactttag tagcatcagc acctgcattt
    20101 tcgagttctc gtacagcttc tggcgtacca acatttccag caatgacaaa tgtttctggt
    20161 aattcagtct tgatatgctt aatcatatca ataacactgt tagcatgacc atgagcaata
    20221 tcaatggtga taaactcagg agcatcctct ttcaaagagg tcacaaattc atattcacaa
    20281 gctttaaccc caacagagat tgatgcaatc aagccttgtt catgcatgcg tttgataaaa
    20341 ggtttgcggc tatcttcatc aaaacgatgc ataatataaa aataaccctc ttttgccaac
    20401 cgctcagcaa ttgtttcatc aataatggtt tgcatatttg ctgggataac tggtagcttg
    20461 aactggtatt ttccgagtgt cacacttgta tctgcttgtg aacgactagt aataatgcac
    20521 ttgttaggaa tcagttgaat atcttcataa tcaaatacag ggatgtcatt aaacataagt
    20581 ttctcctctt ttaacataga ttaagaaaaa caagtcagtt ctcaccttct tctctaagtc
    20641 ctctatacaa aataatcttc ctatgacctc tgatgattag aggtatattt aggtaaaaac
    20701 ttacctttca ttttatagat tacttatttg tttatatttt gtcaaattta aatactattt
    20761 ttaatttaaa tgttcggttt ttgtatttat gaattgttac ttcttgaact taagagacga
    20821 aaaaatgcca gtcctatgga ccaacatttc tgtttatttc tggattaagc ctgttttcaa
    20881 cagataatct cgagcgacct tattagcatc tgctccttta acagatacct cgtagttcat
    20941 gtcctgcatc tctttttcag taatttttcc cgctaattga ttaagaatgc cttttaattc
    21001 tggatgtttt gttaataagg aagttttcat caaaggagct ccctgataag gtggaaacaa
    21061 ctgtttatca tcttttaaaa ctttcaaatg gtatttcgtg atttcagcat ccgtagagta
    21121 agcatctgtt acttggatat ctcctgattg aattgcttga taacgaagag ctggctccat
    21181 cgtcgccaca gatagctgta atccatattg agattgcatt cccttatagc catctgctct
    21241 gtccttaaat tccagagtaa aaccagcctt taaagtatca gcatgcgctt ttaaatcaga
    21301 aatggtttca atctgatatt ccttagccaa tttttctggc atagcaacag catacgtatt
    21361 ttggtaagca aatggcttaa gaagggtcag tttatcttgc ttagcaatgc cttttttagc
    21421 atcctcatag acctgcttag ggtcattaga caaaggtggt ttgtcgcgta aaagacttga
    21481 tgttatggtt cctgtaaatt caggatagat atcaatatct ccagatttaa gggcttcata
    21541 taagaagcta gttttcccaa aattagattt caactcaaca cttatatctg attgatcttc
    21601 aattaattcc ttatagagat taatcagaat atcgggctct gctcccaatt taccggcaat
    21661 aaccactgta tcttttcctt ttgaaaactg actaagcgcc ataggagtat acgatgctag
    21721 caatgcaagt aaggtgattc caaaactaat cataatccgt cgcaaggaag ctttctcaag
    21781 gtactgtaac aaactattga aaataatagc tagcagagct gaagaaatag cccctatcag
    21841 aatcaggtta gcattattac gatcaatccc caataagatg aaagagccaa gtccaccggc
    21901 tcctatcaag gaagctaaag tagctgtgcc gataatcatg actgctgacg tccgcacacc
    21961 tgacataata acaggcattg caattggaat ctcaaacgtt tttaaccgct cccatttggt
    22021 cattccaaaa gctattcctg cttccacgag acttgggtca ataccattta atcctgtgat
    22081 ggtgttttgt aaaatcggaa aaatcgcata gataactaaa gctgtcactg caggcaacgt
    22141 tccaattccc attaaaggga tgaaaagacc aagcaaagcc agtgagggaa tggtttgaaa
    22201 aacacctgtt acctgtaaca taatgtctga ccaacgtttg ctgcgactga gaagggctgc
    22261 taaaggcaca cctattagca aggcgatcat aagagataaa agggaaattt gcaggtgttc
    22321 ccccagagcc gccagccatt cattaaaacg gttttggaaa gttacaaaca aactaggcat
    22381 gctcatcacc tccaaataaa ttggccacaa aagcattagc agggtgggtc ttaatcattt
    22441 ctgggcgatc cagttgcaca atttctccct catttaaaat agctacgcgg tctcctaatt
    22501 taatggcctc atcaatatca tgtgtcacaa atacaatcgt catgtcaaat tccttgtgaa
    22561 gactcaacat taactcttgc aattgtttac gtgaaatagg atctaaggct gaaaatggtt
    22621 catccatcaa tagaatttta ggatgagaaa tgatagcacg tacaatacca atacgttgct
    22681 gttcacctcc cgataaatca cttgggtaac gatcaagata atctttagca ggtaggccca
    22741 ccttatccaa taattcttca gttttttgtc taatttcttc agctgaccat tgcttcatct
    22801 cagggataat cgcaatattt tcagctactg tgagattggg gaaaagagca atttgctgca
    22861 acacgtatcc aatagatagc ctcatctcac gtaaatctaa ctctttctgt gggacgttat
    22921 tcagtaaaat atctccagag cttggttcaa taaggcagtt aatcattttt aaaagtgtgg
    22981 ttttccccga tccgctggga ccaaccagca caaaaaattc acggtcatta atttgaaagg
    23041 tctgctcttg caaaaccttt gtctgaccaa aagttttaga aacattatta aaacgtatca
    23101 ttatttctcc aattcattgg ttaaagtaac agcataaaag ctgatgagtt cattcaacct
    23161 gtctctctgt cattttttct caaaacgttc tgagtaacta caaggaatca ctgcatgccc
    23221 taccattttt agacaaacgc atctactaat taaagggact cttaggtatc ctcagtaagg
    23281 tagaaactac caattctcaa aaactttttc tctcattatt ttcttgtcgt ttgttgttga
    23341 ccattataaa aagtttccca ctataagtca aataatgaga ctatttctcc ttacccctta
    23401 tagaaaaaca ggtcactttg tcagcctatc tctccaaaat taccttatca aaaatcctag
    23461 acttgcctat gatagataag cagattagtt gtcttttcta gaaaatacgg ctatcataca
    23521 attatgaaaa atgccatttc cattttagcg ctcttaacat ctgaaaacca acttgaagat
    23581 atgtttatta gagagctttc ccaccacttc tcacaccttt ttgaggtgac tattctcact
    23641 agcaaagcca acattgagtc aaatcaattg tcgactttcc aaggaatttt cagtttccat
    23701 gagcatgaca ttgacttgcc tacactctat ttcaaaacct ctcaacatgg tcagggattt
    23761 ttagtgacag aaagtgtctt tgatcaagca acagcagtct tatcgttaag ccaatacctc
    23821 actagatttt accaaaaatt tgatagacat tttttacagt accttccttt gcaagcaagg
    23881 ttatctgatg caaatggaaa catcatagtc gataatcatg cttttaatgg aagttttttg
    23941 ccaactactg ataaagaaat agaagattgg attcttgctg aattgcgttt atcagataac
    24001 ccttgtaaaa ccttcctact tccttcgggt tcattggatc atatttacat gcagcattat
    24061 caagctctta aaaatcctca aggtcagctg gtgggagttt tggataccgt tcaagacatt
    24121 aaaccgttat taaaccagta tttagaagaa actggacaag ccattgttgg ctggtccgat
    24181 gtcacatcag gcccttccat tttagatcac taaacttgat tttaaatagg gagttcagcc
    24241 taaatcatca acgcacaaga agcaatcatg aacgcttgaa gacctcaaaa ccttcttctt
    24301 gattgctttt tttgatgact aaatcggtaa gtctcactta taaagaataa tccataccag
    24361 taaaccattt ttctaaagat tattattctt atggactcat cccttgctag tcacgactaa
    24421 ctggttgcgg tccataactt tgcgacactg gcatcagctc aatacgatta acattaacat
    24481 gagggggctg atgaatcacc catgatacag tcgacgcaat atcttgaggt tgaatggcat
    24541 gtgctccttg ataaatggcc ttcacccgtg cctcatcacc attaaagcga acctttgaaa
    24601 attctgtccc gtcacataga ccgggttcaa tattggtcac acgtatatgg gtacctgcta
    24661 aatcagctcg taaattgagt gagaactgct taacaaaagc cttgctagcc ccataaatat
    24721 tagctcccgg atagggaatt gtccctgcgg ttgaccctaa attaataata ataccatcat
    24781 ctttggagac catatgtggt aataattgac gtgtgagata aatcaaccca acaatattag
    24841 tgttaatcat cgtcatccaa ttctcaaaat cggcttcata agccttatca agtcccaatg
    24901 ccaagcctgc attattcact aaaatagtaa tatcttgcca ctgatttggt agacttgtca
    24961 aagcatcatc aatcgctttg gtatctgtga catccatctg caaagggtaa aagttgtccg
    25021 acccaaagct cgtttctaag gccttgagtt tctcaagacg acgagaagca ccaataacct
    25081 ttatcccatc tgcaattaat tgttcgacaa tggctctgcc aaaaccggct gaagcccctg
    25141 ttactaaggc aatatttccc atacatttcc tcaccttcaa aaaaaacgag atggtctggc
    25201 caactcgttt ttaataatgt caattagtct taggataact tctaacacat cacttagcgg
    25261 ttagaatcct atatcaaggg gtcttaagtt acccttggct ttcgatagct tgtgctgctg
    25321 taataatagc taatttgtaa atatcttcag cgcttgagcc acgagataaa tcattaacag
    25381 gtttgttcaa tccttgtaag attggtccaa tagcatcaaa cattcctaaa cgttgtgcaa
    25441 ttttgtaacc aatatttcca gattgtaagt cagggaaaat aaaagtattg gcttggccag
    25501 caaccgctga atctggtgct ttgatagctg ccgtttcagg aacaaaggct gcatcaaatt
    25561 gcaactcacc atcaagtgcc aaatcagggt tcaacccttt agcaatttct gttgcttctc
    25621 ttaccttatc aacttgaggc gctttaccag aaccttttgt tgagaagctt aacatagcaa
    25681 ttttaggatc aatatcaaag attttagctg tttcagctgt gttaacagcg atttctgcca
    25741 attcttgtgc tgttgggtca atattgatag cacaatcagc aaacacgtaa cgctcactcg
    25801 tattttcgcg attcatcaag aaaacacctg aagttcttga aatacctggt ttggttttaa
    25861 tgatttgaag ggctggacga acagtatcag ctgttgaatg aatagcacca gataccattc
    25921 catctgctaa tcccatttta accaacataa caccaaagta attcacatca cgaagcattt
    25981 tatcagcatc ttcaagcgtt gcttttcctt tacggatgtc aacaaaagct tctttcattt
    26041 tgtcaaaatc cgcatattca tttggattaa tgatagtata gtcttgatca gcgaagccta
    26101 attttgtcaa aaggttgcgt acctcttctg actgtccaag gataatcggt tctaagagtc
    26161 cttcaaattt caaacgagca gctgcacgga cgacgcgctc atcattacct tctgggaaaa
    26221 caatcttcat gtttttacca agaatttttt ctcttaaacc accgaataaa ctacgaatac
    26281 tcattcttta ataacctcta tcttttttct gtaaacctat gataacgcta tcaaagtcat
    26341 ctgtcaagtg tattgcatag ctacaatcac tttctgtaaa tggatctttg aaattcaaat
    26401 aatgacaatg taaagcttgc cttgttattc ctaagtccaa acgacctccg taaaggtcat
    26461 cccctaaaag tggaaaacca atatgggcaa aatgaactct gatttgatgg gtacgtcctg
    26521 tatgcaattt gatatccact aaatgcacat tttctgaata gcgcgccaca accttatagc
    26581 tagtcttggc atatttgccc atagggtcaa ctgccctggt aataatacta tcttttgaac
    26641 gcccaattgg agcaataata tctccttcat ctggcaacgt cccatttcca gaaaccagag
    26701 caaaataacg tttctctatt gagcgagttt ggagttgctt gtccaacctt gcatgagcat
    26761 aaccatgctt agcaaataac atcaagccac tcgtatctct gtctaaacga gtcacgatat
    26821 gcacttgttg gtctaggtaa tgattgtcca cataatatgc cttgataaag ttagcaatgg
    26881 tattggagtg gatggcactt ggaatactgg caaagccaat gggtttatta atcaccaaaa
    26941 aatgatcatc ctcgtgaaca atatccaaat caaaagggat ggcttcaagc ttttcaaagg
    27001 gctcttcgtt tggaatgtca atggtgacaa catctcccac ttgcaataaa taaatggcat
    27061 tttgttcaat accattcact aatatattac cgcccttata tttgatttta gccaatagtc
    27121 ctttagagac atcatgactt tttaaaaggg tttttacttt tattcgtttg tctgctacaa
    27181 attcaaatct catgagtcaa tctctccaat aaaggcatcc ttgacccttt tccaaaagct
    27241 cgtatgactc ggagaggaaa caaaatgaat tttctcatcg tcgataaaat actccacctt
    27301 cgtcacattt tttaactgat aggttttatt atcaatggaa atggtataaa ttcctaatcg
    27361 ttttggcact aactcaatct tatctttttt gggaataatg attgatgagc ccaaggttct
    27421 aaagacacgg ttattaagac tggaaatttc cgtcaattgc agcgcttcaa tcgtcggatg
    27481 caagacagca ccacctaaag atttattgta ggctgtgctc cctgtcgggg tcgatactga
    27541 aatcccatca cctcggaagc tttcaaattt gacatggtta ataataacat ctgctaccat
    27601 cgttttttca atacgcttaa ccgtcgcttc attcaaagca cgcgctttaa ccacacgacc
    27661 atcatctaaa gtaatagcaa cttttaaaat aggataagag atttgttctc ccttgtcttt
    27721 tcttaaatta tcaatcaatt tatcaacttc aaaatcccta taatcggtat aaaagccaag
    27781 atgaccggtg tggattccta caaaacgtac cttatctaat tctttttcat acatgtgaaa
    27841 ggcagataag agcatcccat ctccgccaat agaaatcaca atatccggat ttttctttga
    27901 aagatagaaa tcaggatcat ctttaaatac ggagaaaagt ttggaggcga cgcgtttact
    27961 ttggtactta ccatttgcaa taatagcaac tcgctttacc ttacctgtat aattcatctg
    28021 tgtcatcact atttcctaca ccatcactta aatttcgtgt cacggggtca aacaataatt
    28081 gagcctcccg aatatcttct cgaatcttac gcatttcttc gtctaattct aaagcaattt
    28141 tagcagtcac ttcaagacgc tttttaattt cttctgggaa gtctccacca tatttataat
    28201 ttaaagaatg ctcaatggtc gcccaaaagt tcattgccaa agtacgaatc tgaatctccg
    28261 ctaagacctt tttttgacct tcaatagtat caacagggta ctcaaccaca acatgataag
    28321 acctgtagcc actggacttc atattgcgaa tataatctcg ttcataaaca attgtcatgt
    28381 cttgtcgttg acgcaataga gctaagactt cttccacatc atcgacaaac tgaaccataa
    28441 tacgtagtcc agcaatatct tgaatatctt gtgctatatt ttcttcaata acaccacgta
    28501 aaatcatttt ttctttaata ctttcaattg acttaacacg tcccgtaaca aattcaatcg
    28561 gagaataacg attttgctta cgatattgct tgcgaatacc ccgtagtttt atttttaatt
    28621 caccaactgt ttggatataa ggatctaaaa attcttccca atctaaggtc atctattgtc
    28681 tcctttcttg tcaaagccag gataataata taaaattgaa ataacaattt attatagcac
    28741 aaattaaaag gaatttccca tgactaacct agaaatcgaa tataaaactc tacttacaaa
    28801 aaatgagtat aaccgtcttt tatctcaaat gaaacatgtg acgcctgtta cacaaactaa
    28861 ttactatatt gatacaaaag catttgattt aaaagctaac aaaatgtcat tacgtattcg
    28921 aacatttgca aatagcgccg aattaacctt aaaagtgcca gaaaaagtgg ggaatcgtga
    28981 gtacaatgtc cctctttttc ttgaacaagc aaaagatatg attaaacatg ggaatcttcc
    29041 tgaatcaact gctcttgata ttattatttc aaaaggtatc aaaccatctg cactggtaac
    29101 ttttggcaat ttaactaccg ttcgtcgcga gacagtgata ccgataggta aattggcctt
    29161 agactataac ctatatgcca ataccaaaga ctatgaatta gaattagaag taagtgacgc
    29221 tcttcaagga aagattgatt ttgattcttt tttatctgaa catcacattg ctttcaaata
    29281 tgctaaaagc aaagtagctc ggtgtattaa caccttaaaa aagttcaacg ataaataatg
    29341 gctaaaagcg acagaaaaac tgaaattttt ctgtttttct gttaaaatag ggatgtcaat
    29401 taaacacttt tgggtaatgt gactcattaa taaaacagtt tacccaattt tattgaagtt
    29461 aaggagctaa atctatcatg actgaacgat atgctgacaa gcaaatcaaa ctgttctcac
    29521 tcacatcaaa tcttccaatt gctgaaaaaa ttgctaaagc tgctggaatc cctcttggaa
    29581 aaatgtcttc acgtcaattt tccgatggag aaattatgat caatattgaa gaaacagtgc
    29641 gtggagatga tatctatatt attcaatcca ctagttttcc tgtcaatgat aatctttggg
    29701 aattactcat catgattgat gcttgtaaac gtgcaagtgc taataccgtc aatattgtat
    29761 tgccatactt tggttactct cgtcaagacc gtgtcgctaa gccccgcgaa ccgattactg
    29821 ctaaactagt agctaatatg ctgactaaag ctggtattga tcgtgtagtg acgcttgact
    29881 tacatgctgt tcaggtacaa ggtttttttg atattccagt ggataacctc tttacagtcc
    29941 ctctttttgc agaacgttac agtaaattag gcttatcagg ttctgatgtt gttgtcgtta
    30001 gccctaaaaa ttctggaatt aaacgtgcta gaagcttggc tgaatatctt gattccccga
    30061 ttgctattat cgactatgca caagatgatt ctgaacgtga acaaggttat attattggtg
    30121 atgtttctgg taaaaaagcc attttaattg atgatatttt aaatactggg aaaacctttg
    30181 cagaagctgc taaaattctt gaacgctcag gcgctactga tacttacgcg gtcgctagcc
    30241 atggcttatt tgctggtgga gctgctgagg tattagaaac agctccaatc aaagaaatta
    30301 tcgttacaga ttctgttaaa actaaaaatc gtgtgccaga aaacgttact tatcttagcg
    30361 ccagtgattt aatcgcagaa gctattattc gtatccatga aagaagaccc ttaagtccac
    30421 ttttttctta tcaacctaaa ggcaaaaata acgcatgact tactttgata acgccgccac
    30481 cacaccactt agtcctaatg tgattagggc gatgacagca gctatgcaag ataactttgg
    30541 taacccctct agtattcatt tctacgggcg ccgagccaat aaaatccttc gtgaatgccg
    30601 acaagctatc gctagaaact tgggggcaag tgaacagcaa attatagtaa cgtccggtgg
    30661 aactgaaagc aataatatgg ccataaaagg ctatgcttta gctcatcaag caaagggcaa
    30721 acatctcatc acaactacta ttgagcacca ctctgtgctt cataccatgg cctatctcga
    30781 agagcgattt ggttttgagg tcacttattt gccttgtcaa aatggacaaa taaatttatc
    30841 tgacttaaaa caagccttga gagatgacac cattttggta agcataatgt atgccaataa
    30901 cgaaacaggt gacctgttac ctattaagga cattgggaat ctgctcaaag accatcaagc
    30961 tgcttttcat gttgatgccg ttcaagctgt tggcaaactc aagattattc ctagcgaact
    31021 tgggattgac tttttatctg cttctgctca caaatttcat ggtcctaaag gatgcggctt
    31081 tttatacagt aacggacaac caatcgaccc tcttcttcac gggggtgatc aagaaggaaa
    31141 gcgacgcgca agtactgaaa atatgttagg tattattggt atggcacaag ctttgactga
    31201 tgctatgact tgtcttgacc aatcaactga tcacattatc agtttacgtc atcacttgat
    31261 tagcctccta gaaggactac cctactatat taatcaaggt actcattatc ttcctcatgt
    31321 tctcaatatt ggatttttag gctaccaaaa tactatttta ctaacacaac ttgacttagc
    31381 aggcattgca gtatcaactg gttctgcttg tactgctgga gctgttaatc caagtcatgt
    31441 tttagctgcg tactatgggg atgactcttc tcgtttaaaa gaatcaattc gtatcagttt
    31501 ttcagatcaa aatagcattg aagatgttaa tcaattagct caaacattaa aaaatatttt
    31561 aggaggaaca gatggctttt gaaaaagaaa ttaagcttaa agactgtaaa tacctttata
    31621 caatcagtcc aaacattaaa aaatataccc taagagatac tacatttagt caaactaaag
    31681 tagggcacta ccaattaata cgtttactcg aaaaaatacc taattcaggg gatggtttcc
    31741 ctttaaaaat cactattaat aaagaactca caggtt
//
LOCUS       DATUYR010000016        30438 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.016, whole genome shotgun sequence.
ACCESSION   DATUYR010000016 DATUYR010000000
VERSION     DATUYR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30438
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.016"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..284)
                     /locus_tag="VCI33_000676"
     CDS             complement(<1..284)
                     /locus_tag="VCI33_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2831614.1"
                     /translation="MKRLIKTIILVIIIFHGYGSVKSDSEKIKDVKLQLNYAYEIRPV
                     DYTNCNIDYLTTHDFYIDVSSYKKKNFSVDSEVESYITTKFTKNQKVNIFG"
     gene            complement(486..623)
                     /locus_tag="VCI33_000677"
     CDS             complement(486..623)
                     /locus_tag="VCI33_000677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831615.1"
                     /translation="MLTYHELKKAVANRYIKGDMISITRKLVSSLILSFLIELYEYQE
                     F"
     gene            855..1138
                     /locus_tag="VCI33_000678"
                     /pseudo
     CDS             855..1138
                     /locus_tag="VCI33_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1312..1893
                     /locus_tag="VCI33_000679"
     CDS             1312..1893
                     /locus_tag="VCI33_000679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831616.1"
                     /translation="MKTRPKRFLNLATLSLALLGTTLLMAQPVKADNDTRESQERVDQ
                     RIEDPYDDGKNAGRKDGYESGNHKGISEEPPTKTPEPPTNPYSRDRVKSKKYRDGYQE
                     WYVYGYYEGWHRANDEDKNKESSDHSRDQDLEGTEQTDGQDEDKNKESSDHSRNQDLE
                     GTEQTDTEYSAIISTLYGIAGVVLEYVLSWFSI"
     gene            2553..2789
                     /locus_tag="VCI33_000680"
     CDS             2553..2789
                     /locus_tag="VCI33_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEP2831617.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            2782..3480
                     /locus_tag="VCI33_000681"
     CDS             2782..3480
                     /locus_tag="VCI33_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEP2831618.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLRQGYHVYGVDKSDKPDLSGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLAEWVARETPIGRWTEPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            3556..4515
                     /locus_tag="VCI33_000682"
     CDS             3556..4515
                     /locus_tag="VCI33_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP2831619.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLATSEYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLNNMTFIKEHLDQLGDIKIVECNYSQYSSRYDVF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKTTIQGNKGSLAVLGATNTLPQVQLSLHGHEAQVVNHNKY
                     DHRMYEEFVAFRDMIDQRDFEKVNQALEHSRAVMAVLERAVHS"
     gene            4848..6185
                     /locus_tag="VCI33_000683"
     CDS             4848..6185
                     /locus_tag="VCI33_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2831620.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISMLEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            6358..7740
                     /gene="glmU"
                     /locus_tag="VCI33_000684"
     CDS             6358..7740
                     /gene="glmU"
                     /locus_tag="VCI33_000684"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEP2831621.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVTIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDHALTAAGSTISKTVPIDSIAIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            7771..8325
                     /locus_tag="VCI33_000685"
     CDS             7771..8325
                     /locus_tag="VCI33_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP2831622.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKILLVKQYRKAIERVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            8325..8576
                     /locus_tag="VCI33_000686"
     CDS             8325..8576
                     /locus_tag="VCI33_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831623.1"
                     /translation="MGRPLLTDDIIEKARRMETFESDDAVDFDTKVMTLPEKDDKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            8596..9291
                     /locus_tag="VCI33_000687"
     CDS             8596..9291
                     /locus_tag="VCI33_000687"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEP2831624.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENLPV"
     gene            9442..9783
                     /locus_tag="VCI33_000688"
     CDS             9442..9783
                     /locus_tag="VCI33_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831625.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGRYRQGLTD
                     EKSKLQYSKDNAKLQLRNILNGYQSDLGRHYSSYYYYNLRTVMGLSKEQEIEKYYEEL
                     KNKLHDMYNHY"
     gene            complement(9882..10529)
                     /locus_tag="VCI33_000689"
     CDS             complement(9882..10529)
                     /locus_tag="VCI33_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEP2831626.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLSINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
     gene            10674..11606
                     /locus_tag="VCI33_000690"
     CDS             10674..11606
                     /locus_tag="VCI33_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEP2831627.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            11670..12395
                     /locus_tag="VCI33_000691"
     CDS             11670..12395
                     /locus_tag="VCI33_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP2831628.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            12396..13250
                     /locus_tag="VCI33_000692"
     CDS             12396..13250
                     /locus_tag="VCI33_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP2831629.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLVVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(13397..14203)
                     /locus_tag="VCI33_000693"
     CDS             complement(13397..14203)
                     /locus_tag="VCI33_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP2831630.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPKQDITITSIDIVKDYRFKN"
     gene            14420..16825
                     /locus_tag="VCI33_000694"
     CDS             14420..16825
                     /locus_tag="VCI33_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP2831631.1"
                     /translation="MVKRNQRRKSAPKKCLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMPKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVDVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FLMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKVGVRNIA
                     GYNAKVEDYNRQSEQKQMPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(16895..17248)
                     /locus_tag="VCI33_000695"
     CDS             complement(16895..17248)
                     /locus_tag="VCI33_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEP2831632.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTPLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            17492..17917
                     /gene="rplK"
                     /locus_tag="VCI33_000696"
     CDS             17492..17917
                     /gene="rplK"
                     /locus_tag="VCI33_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEP2831633.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            18023..18712
                     /gene="rplA"
                     /locus_tag="VCI33_000697"
     CDS             18023..18712
                     /gene="rplA"
                     /locus_tag="VCI33_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEP2831634.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            19034..19762
                     /locus_tag="VCI33_000698"
     CDS             19034..19762
                     /locus_tag="VCI33_000698"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEP2831635.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            19791..20348
                     /gene="frr"
                     /locus_tag="VCI33_000699"
     CDS             19791..20348
                     /gene="frr"
                     /locus_tag="VCI33_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEP2831636.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAIKEIDRMTAEKEKELLSV"
     gene            20457..21314
                     /locus_tag="VCI33_000700"
     CDS             20457..21314
                     /locus_tag="VCI33_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP2831637.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            21387..21896
                     /gene="msrA"
                     /locus_tag="VCI33_000701"
     CDS             21387..21896
                     /gene="msrA"
                     /locus_tag="VCI33_000701"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEP2831638.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPEEISYKELVELYWVQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPRRYAQSSAIR
                     HQFLEENWS"
     gene            21893..22108
                     /locus_tag="VCI33_000702"
     CDS             21893..22108
                     /locus_tag="VCI33_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEP2831639.1"
                     /translation="MRKSFYSWLMTQRNPKSNAPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            22264..23445
                     /locus_tag="VCI33_000703"
     CDS             22264..23445
                     /locus_tag="VCI33_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP2831640.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTQIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTIPMAPSATPSDVPTTPLASAKPDSFVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEASSAAPAQAPAEKEETSQMLTAPAAQKAVADTTSVATSNGLSYAPNHAY
                     NPMNAGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPVSSTLGDQV
                     AQYAIDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFN
                     A"
     gene            23760..25532
                     /locus_tag="VCI33_000704"
     CDS             23760..25532
                     /locus_tag="VCI33_000704"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEP2831641.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELVHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            25691..26743
                     /locus_tag="VCI33_000705"
     CDS             25691..26743
                     /locus_tag="VCI33_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEP2831642.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            26789..27364
                     /locus_tag="VCI33_000706"
     CDS             26789..27364
                     /locus_tag="VCI33_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEP2831643.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARLIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            27523..28020
                     /gene="ybeY"
                     /locus_tag="VCI33_000707"
     CDS             27523..28020
                     /gene="ybeY"
                     /locus_tag="VCI33_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEP2831644.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDFAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            28001..28408
                     /locus_tag="VCI33_000708"
     CDS             28001..28408
                     /locus_tag="VCI33_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEP2831645.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            28528..29424
                     /gene="era"
                     /locus_tag="VCI33_000709"
     CDS             28528..29424
                     /gene="era"
                     /locus_tag="VCI33_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEP2831646.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            29444..29920
                     /locus_tag="VCI33_000710"
     CDS             29444..29920
                     /locus_tag="VCI33_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP2831647.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(30226..30405)
                     /locus_tag="VCI33_000711"
     CDS             complement(30226..30405)
                     /locus_tag="VCI33_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831648.1"
                     /translation="MRYPHAHIILIGNHINYEEIFKNHYRVFGVIDTTSHKSLKSIRD
                     QIQLYLDELYNSNNG"
BASE COUNT         9345 a         5342 c         6637 g         9114 t
ORIGIN      
        1 ccaaaaatat ttactttttg atttttcgta aactttgttg taatatagct ctcgacctca
       61 gaatcaactg aaaaattttt ctttttataa ctggaaacat caatataaaa atcatgagtg
      121 gtcaagtaat caatattaca attcgtataa tctactggtc tgatttcgta tgcgtaattt
      181 aattgtagct taacgtcttt aatcttttca ctatctgatt ttacacttcc gtacccatga
      241 aagattatta taactaaaat tattgttttt attaatcttt tcatacctct cctcgctcac
      301 cccgtgcccc acaaactact ttatcctaaa aaacaataaa atcaacattg aggacttttt
      361 tggttcccat aattgttaac tatgtcattt tagagcaatc atatagagac taagatataa
      421 ttttaccaaa gttttgaagg ctattgaaga gtgactttct cccttaattt atttaattaa
      481 agatactaaa attcttgata ttcataaagc tcaatcagga aggacaaaat caaagagctt
      541 accagttttc tggtaatgct gatcatgtct cctttgatat acctgttagc tacagctttt
      601 ttcaattcgt gatatgttaa catttttatc ctcctactta atctattcgt aaaaaatctc
      661 ttaaaataga catttaaaaa aaagtccatt ataacggata tgcccgtgtg cgaatacatg
      721 ttcgggtaaa tattttaaat caagatgttt gatgtttgtg tgtgcaaaaa caaaaaaatc
      781 gccctcgatt gatgatatga tacgtcttaa gtagacaggc taaataacca aaaatctact
      841 caagaggtat ttttatgcca gaaaagaaag ggctgctcgt cttaagcatg gtagtgagcc
      901 ttgtgtccct gcttgatcat atcttctttt ttgctactta tagttatctt tacttgccac
      961 ttgtagcaag tagtctagct attattagtc tggtgcaaaa caaaggaagg caaaaggttt
     1021 ggtcgatagc tgctttagcc gtagcagtag tgacctatac ccaacggtta ttccttatca
     1081 aatgcaaagt ccctccctct tatataaact atcaaatact ttcaaaaaca gttcctaaag
     1141 ctcaaaaagg ttatctaact gctccaagca ggcactttct gtgataagga tagcttgcca
     1201 tttttttcta atcatttgat atttaaaatg ataaaaaaat gaacttgaaa atgaaagaat
     1261 gagaggtgta aactaaaagt accaaataaa caatgaatac ggagtgattt tatgaaaaca
     1321 agaccaaagc gctttttaaa tctagcaacc cttagcttgg cccttttggg cacaacctta
     1381 ctaatggcac aaccggtaaa ggcggataat gatactaggg agtctcaaga gcgcgtagac
     1441 caaagaatag aagatcctta tgatgatgga aaaaatgctg ggagaaagga tggttatgaa
     1501 tcagggaatc ataagggaat ctctgaagag cctccaacta aaactcctga acctccgact
     1561 aatccttata gccgtgatag ggtgaaaagc aagaagtata gagatggtta tcaggaatgg
     1621 tatgtctatg gatactatga aggttggcat agagcaaatg atgaggataa aaataaagag
     1681 tcttctgacc attctcgaga tcaggattta gaaggtacag aacagacaga tggacaggat
     1741 gaggataaaa ataaagagtc ttctgaccat tctcgaaatc aggatttaga aggtacagaa
     1801 cagacagata ctgaatattc agcaattatc agtacattgt atggaattgc tggagttgta
     1861 ttggaatatg tgctatcttg gtttagtatc taagatttag ttttggttac tggataatgg
     1921 tttaaatatt tattattatc taaaaatggc gtgatcaaat tttgatgatt tggttaatat
     1981 tgtttagaac agactgaaga caaagctctt agtaactaaa ttctaagggc tttgtcttca
     2041 gtctgaggca ccttacgggg tgtcttttat tatttaggtg gcttaatgaa ttggttaaaa
     2101 tctttttagt ttagtaaaat tgctatttaa ggttagccac ctctcgtatg gtcacagttg
     2161 atgaattatg ctatagtata gacataaaaa atagttcaaa tcccttcttg cctgttggtg
     2221 gattttgaac tgaaagcctc tctgattgca gttggtaggc ttttttgatg ctttttcgta
     2281 ctttttttat ttattacgct aaaaaatgtt atacttgatg aagaaaaagg agagtttaga
     2341 acgttaagat acctcacagc agattgatag aggatatgga aaaaggttag ctttcccaag
     2401 agatgccgtt tgggaatggt ataacaataa ctggcagaag ttataagtaa aacaagaaca
     2461 aattaactca gatgattttt agacgttccc acgcgtgtgc gaattaattg gaaaaaaatg
     2521 agaaaatacg atatacaaaa aaggagaaca atatgacatt tgaagaaatt ttaccaggtt
     2581 taaaagccaa gaaaaaatat gtacgtacag gttggggtgg cgctgaaaat tatgtgcagt
     2641 tgtttgatac ccttgaagtg gatgggaaag tactacaggc gacgccttat tttctgattc
     2701 atgttactgg tgaaggtgaa ggtttttcca tgtgggcacc gacaccatgt gatgtgttag
     2761 cagaggattg gatcgaggtc aatgactaaa gttgttttag tgacgggctg tgctagtggg
     2821 attggttatg cacaggcccg ctattttttg agacaagggt atcacgtata tggtgtggat
     2881 aagtcggata agccggactt aagcggtaac tttcacttta tcaaattgga tttatcctct
     2941 gagttatccc cactctttaa agtggtcccc agtgttgata ttttgtgtaa tactgcaggg
     3001 attttagatg cctacaagcc acttttagac gttagtgatg aagaagtgga acacctattt
     3061 gatattaatt tttttgcgac ggtgaagtta acacgtcatt atcttagacg tatggtggaa
     3121 aagcagtcag gtgttatcat caatatgtgc tcaatagcaa gttttatagc gggtggtggt
     3181 ggtgttgcct atacgagttc taagcatgcg cttgcgggct tcacgcgtca attggctttg
     3241 gattacgcca aagatcagat tcacatcttt ggtatagcgc caggtgctgt caaaaccgct
     3301 atgacggcta gcgactttga gcctggtggc ttagcagaat gggtagctcg tgagacgcca
     3361 attgggcgtt ggactgaacc agatgaagtt gctgaattaa cgggatttct tgcgtcagga
     3421 aaagcgagat caatgcaagg tgaaattgtc aaaattgacg gtggctggac tttgaaatga
     3481 tcacatgcct tagcttgagc tggcgcagta agccctagag gtgtgatgag tcaaacagtc
     3541 aaaggagatg aacaaatgaa attagcggtt ttggggacag gtatgattgt taaggaagtt
     3601 ttgcctgtct tgcagaaaat tgatggtatt gatctagttg ctatcttatc aacagttaga
     3661 agtttaaaga cagctaaaga cctcgctaag gcatatcaca tgcctctagc tacgagtgaa
     3721 tatgaggctg tcttaggcaa tgaggagatt gacacggtct atattggact gccaaatcac
     3781 ctgcattttg cctatgctaa ggaggctctt ctggctggta aacatgtcat ttgtgaaaag
     3841 ccctttacca tgacagcagg agaattagat gaactggtgg taatcgctaa aaaacgaaag
     3901 cttattttgt tagaagcgat tacgaatcag tatttaaaca acatgacctt tatcaaagag
     3961 catcttgacc aacttggcga tatcaaaatt gtagaatgca attattcaca atattcttca
     4021 cgctacgatg tcttcaaacg aggtgatatc gcgccagctt ttaatcctaa aatgggtggt
     4081 ggagctttac gtgatttaaa catttacaac attcattttg tggtaggatt atttggtcgc
     4141 cctaaaacgg ttcaatattt agccaatgtc gaaaaaggca ttgacacatc aggcatgctt
     4201 gtcatggact atgagcagtt taaagtggtg tgtatcgggg ccaaagactg cactgcggaa
     4261 atcaaaacaa ccatccaagg taataaaggg tcactggcag tgcttggagc aacaaatacc
     4321 cttcctcagg ttcagttaag tctacacggt cacgaagcac aagtggttaa tcacaacaag
     4381 tatgaccacc ggatgtatga agagtttgtc gcttttaggg acatgattga ccaaagagac
     4441 tttgagaaag tgaatcaggc tttggagcat agccgagctg tcatggctgt gttggagcga
     4501 gctgttcatt cgtgatggta atctggtagc cattgccttt tgtgcagttg ctatggtaat
     4561 ggttgaaaga cgtgttttca cgagggttaa ccccctcgtg cttttttggt aaaaataact
     4621 tgttcaggat ttgggccttc tttgtgagat gagagatgcc tgacgaattg atttgattaa
     4681 aataatgata aagccaaacg atttaagaaa tgaccgttcg gaaattctgc cccaaaaggt
     4741 ctctagaagc agtaaaatcg aatttgtaag cgttgacact gctgcataac tctgttacac
     4801 taagagaatg tcatgacaaa aattacagtg taggagttta attgtttatg gaagaggatt
     4861 ataacaaaag agaacctgag aagtttacgc aatttctgcg ccgtcaaaag gtcgtctttt
     4921 ttgtcgcatt ttttggttat gtgtgtgctt acttggtacg caacaatttc aaactaatgt
     4981 caaacactat tatggtccaa aacggttggg acaaggctca aattgcgacc ttgttatcgt
     5041 gtttgaccgt ttcttatggc ttagcaaaat tctatatggg ggccttaggg gatcgtgtta
     5101 gcttgagaaa gctcttttcg atcagcttgg gtgcaagcgc tttgatttgt attttaattg
     5161 gttttttcaa tagttcaatg gtagtgttag ggatactgct agtcttgtgt ggtgttgtac
     5221 aaggtgcctt ggcacctgcc tcacaagcca tgattgccaa ttattttcca aataaaacac
     5281 gcggcggagc catcgctgga tggaacattt ctcaaaacat gggatcagcc ctcttgccat
     5341 taaccatcgc cttgctcacc agtatgggct tggtggtacc agctaatggc aatattttac
     5401 ttgccttttt aatccctggt gttttagtgt tcttgtttgc tttgtgttgc tggaagcttg
     5461 gcggtgataa tcctgagtct gagggacttg actctcttcg aacgatgtat ggtgatgcgg
     5521 gcgaatcagc tgttgccagt gaagaagaaa agcataacct gtcttattgg caactcatct
     5581 ggaaatacgt tttttgtaac ccgtcgctct tacttgttgc tgctgtcaat gtggcccttt
     5641 attttgttcg ttttgggatt gaagactgga tgccgatcta cttgtcacaa gtagccaata
     5701 tgtcagaggc ccatattcat tttgcgattt caatgttaga gtgggtagct attccaggct
     5761 cgctggtatt tgcgtggtta gcggttcgtt atcctaataa aatggccaag attggggcta
     5821 ttgggctttt tgtgttagcg gctattgtgt ttgtctatga acgcttgact gccacaggtg
     5881 ctccaaacta tttcttgttg cttgttattg caggtatttt agggtcattg atttatggcc
     5941 cacagttgat cgtgaatatt ttaacaatca actttgttcc tttaaacgtt gcaggaacag
     6001 cgattggttt tgtaggagta acagcttatc tcatcggcaa tatgggagca aactggctga
     6061 tgccgatttt ggcagatggt tttggctggt tttggtcata tattgtcgtt gcagccttat
     6121 ctgctttttc agcggttggt tatttaattt tagccaaacg tgaggaagaa atcatcaaag
     6181 attagctagg caaatgccta agaaagacag acttattgct gaagtcctgt cttttttagt
     6241 catcacaagc cgcaagtaac actataaaag acaagaaaat gctagggtgt ttcaggaaaa
     6301 aaagagaaaa tatgctataa tgagaaggta tttaaattat atagtaagga aaaacaaatg
     6361 acaaattatg ccattatttt agcagcgggc aaagggactc gcatgacttc tgatctccca
     6421 aaagttcttc ataaagtatc tggactaacc atgcttgagc atgtttttcg tagtgtaaaa
     6481 gcgattagtc ctgaaaaatc cgtgacggtt attggtcaca aatcagaaat ggtacgtgct
     6541 gtcttagcag atcaatcagc ctttgtccat caaacagagc agttaggaac tggccatgcc
     6601 gtgatgatgg cagaaacaca actagaagga ctagaagggc ataccttggt gattgcagga
     6661 gatactccct tgatcactgg agaaagcctg aagagtctga ttgattttca tgtgaaccat
     6721 aaaaatgtcg ccactatttt aacagctacg gctcaagatc catttggtta tggtcgtatt
     6781 gttcgcaaca aagatggcga agtgatcaag attgttgagc aaaaagacgc caatgagtat
     6841 gagcaacaat taaaagaaat caatacagga acttatgtgt ttgataacaa gcgtctcttt
     6901 gaagcgctca aatgcatcac taccaacaat gctcaaggag agtattacct gactgatgtt
     6961 gtgactattt ttagagcaaa caaagaaaag gtaggagcct atatcctgag agacttcaat
     7021 gaaagtcttg gtgttaatga tcgtgtggcc ttagcaacag ctgaaacagt gatgcgccag
     7081 cgcattaccc aaaaacatat ggtcaatggg gtgactttcc aaaatcctga aaccgtttat
     7141 attgaaagcg atgttgagat tgctccagat gtccttatcg aaggaaatgt gaccttgaaa
     7201 gggcgtacac atatcggctc aggaactgtc ttgacgaatg gtacttatat tgtggattct
     7261 gaaattggtg ataactgtgt tgttactaat tcaatgatcg aatcatctgt tttagctgca
     7321 ggagttacgg ttggccctta cgcccacctt cgaccaggaa cgaccttaga tagggaagtc
     7381 catatcggta actttgttga ggtcaagggt tctcacattg gtgaaaaaac aaaggctggg
     7441 catctgactt atattggaaa cgctcaggtt gggtcttcag tcaatgttgg ggctggaacc
     7501 atcactgtca actacgacgg ccaaaacaaa tatgaaacag tcattggaga tcacgctttt
     7561 attgggagca actcgactct cattgcacct ttggaagttg gggatcacgc tttaacagca
     7621 gcaggttcaa cgatttcaaa aacggttcct atcgatagca ttgctattgg tcgtagccgc
     7681 caagtcacaa aagaaggtta tgccaagcgc ctagcgcatc acccaagtcg aagcaaataa
     7741 ttcaagatct atttagtaag gaggccaggc atgaaatttg aagaaaaaac gcttaagaga
     7801 caaacagttt ttgatggtca tatttttaaa gttgttgtgg atgatgttga acttcccaac
     7861 aatctggggc aatccaagcg agaattaatt tttcatcgtg gtgccgttgc tgttttggct
     7921 attactcctg aacgtaagat tctccttgtc aaacaatacc gtaaggctat tgaacgtgtc
     7981 tcatacgaga tcccagcggg taagctagag attggtgagg aaggctcaaa gctaaaagct
     8041 gctgcacgtg agctagaaga agaaacagcc tatacgggga cattgacgtt tttgtatgaa
     8101 ttttatacag cgattggttt ttgcaacgaa aaaatcacac ttttcttagc gacagaccta
     8161 acacaagttg ccaatcccaa acctcaagac gacgatgaag ttatcgaggt cttagagttg
     8221 acatatcaag agtgcatgga cttagtcgct caaggtaaac ttgctgacgc taaaacgttg
     8281 attgctttgc aatactatgc cttacatttt ggaggtgagc agtgatggga aggccattgt
     8341 tgaccgatga tatcatcgaa aaagcgagac gaatggaaac atttgagtca gatgatgctg
     8401 tagactttga cacaaaagta atgacgctac cagaaaaaga tgataaagca cgcatttata
     8461 aaagccgtcg gattgaaaat gccaagcgca gccaattgca atctaaatta aatgtgattt
     8521 tgatagctgt tatgctgtta attgccatct tggtatatgc tatcttttat ctttaaaaaa
     8581 acgtaaggaa atgatatgaa aataggaatt attgctgcta tggaggagga gttgtccctg
     8641 ctcttagcaa atcttttgga tgctcaagaa catcaggtgc tttcaaaaac ttactatacg
     8701 ggtcgttttg gcaagcatga gctcattttg gtacagtcag gtgttggaaa agtgatgtct
     8761 gctatgacag ttgcgatctt ggttgagcat ttcaaagccc aagctattat caatacagga
     8821 tcagcagggg cagtcgcttc acatttagcg attggagatg tggtggttgc agatcggtta
     8881 gtgtatcatg atgtggatgc tactgcattt ggttatgctt atggtcaaat ggctggtcaa
     8941 ccattatact atgattgtga tccacagttt gtggctatct tcaaacaagt cctaaaacac
     9001 gaaaagacaa atggccaagt tggtttgatt gcaacaggag acagttttgt tgctggtcag
     9061 gacaagattg accagatcaa aacagctttt agcgacgtgt tagcagtaga aatggaaggc
     9121 gcagctattg ctcaagcagc acatactgca gggaaaccct tcatcgttgt tcgtgcgatg
     9181 agtgataccg cagcgcatga tgccaatatt acttttgatc aatttattat agaagctggc
     9241 aagcgttcgg cccagatatt aatgacattt ttagaaaact tacctgttta actcacctta
     9301 tcgtgagaca attagctgga taatctctat tttaggagtc tggtcacttc ttgttttgaa
     9361 acctaaaaat aagaattgca atcacttgac caaacgctat aataggttaa gtaacgatag
     9421 attactaaaa ggagtctaaa aatgacacta aaaaaacact attatcttct cagtctgcta
     9481 gctcttgtaa cggttggtgc tgcctttaac acaagccaga gtgtcagtgc acaagtttat
     9541 agcaatggaa gatatcgtca aggtttgact gatgaaaaat caaagctgca atatagtaaa
     9601 gacaacgcca aacttcaatt gagaaatatc cttaacggct accaaagtga cctagggaga
     9661 cactactcta gctattatta ctacaaccta agaaccgtta tgggactatc aaaagagcaa
     9721 gagattgaaa aatattatga agagcttaag aacaagttac atgatatgta caatcattat
     9781 taagttaaag gcttaaaaat ggagactagc ccctcaaaaa aagaggccac actcatttaa
     9841 atttaaatga gtgtggcctc tttttgagga gacgcaaggg gttaaagggc tgtgacatag
     9901 agttgcttag caatattttc agggatgaca agttccttgt cgccataaca aatcgtatag
     9961 gtttttgcaa aggtatcaat ttgagttaag gttagctcgg tattgatact caggtgatgt
    10021 gcttctagat actggattaa atcaaagtgg tcgtggattc tgcttaggcg aaaccgtccc
    10081 aactccgtga tggtgttgag agttgtagtg ttcatttcga caagaggctg accatagcga
    10141 ggaatagtcc ctccgtgtgg gcagaaatct ggaaaatcaa ggatcttgtc taaacggtcg
    10201 ataaaggtgt ctgagacagt gtgctctaag acttcagctt cttggtgcac ttcttgggta
    10261 ttatagccca gctggtgaat caaaaatacc tcaatcaagc ggtgtttgcg gtagaggtta
    10321 gcaacaagag catagccttt gtcttttagc aaatagcctt ttgctttatc tttgacaatc
    10381 cagccttgtg aaatcatttt tttaatcatc tctgaaacgg caggtgccga aacgtgcatc
    10441 ttttctgcaa ccattttatt tgtaatcttg ggttcttgtt ctccaatttc atagatacat
    10501 ttcaagtaat cttctttatt aggcgtcata tgtttggcct ttctcttatt tataatgatt
    10561 attatatcag aatttttagg aaaaagcttg acaaaaagcc taaaagagga tatccttata
    10621 gcataattaa ctaaacttaa ttaaataaat aatatatcct aaggagagaa atgatgggaa
    10681 agagaatgag cctcattttg ggtgcttttt tatctgtttt tcttttagta gcttgttcgt
    10741 caactggcac taaaactgct aagagtgata aattaaaagt cgtggcaacc aattcaatta
    10801 ttgccgacat gacaaaagct attgctggtg ataaaatcga tctgcacagc attgtgccaa
    10861 tcggtcaaga ccctcatgag tacgaaccat taccagaaga tgttgaaaaa acaagtaatg
    10921 ctgatgtgat tttctataat ggtatcaatc tagaagatgg tgggcaagct tggttcacca
    10981 aactagtgaa aaatgctcaa aaaacgaaaa acaaagatta ctttgccgtg tctgatggca
    11041 ttgatgtgat ttacttggaa ggtgcaagcg aaaaaggaaa agaagatcca catgcttggt
    11101 taaatctcga aaacggaatc atttattcaa aaaacattgc caaacaattg attgcaaagg
    11161 atcctaaaaa caaagaaact tatgaaaaga acctaaaagc ttatgtggct aaattggaaa
    11221 aactagacaa agaagccaaa tcaaaatttg atgctattgc agaaaataaa aaattgattg
    11281 tgactagtga aggctgcttc aagtactttt caaaagctta cggtgtccca tctgcttata
    11341 tctgggaaat taacaccgaa gaagaaggaa caccagatca aatttcatca ttgattgaaa
    11401 aactaaaagt catcaagcca tctgcgcttt ttgtagagtc aagtgtcgat agacgcccta
    11461 tggaaaccgt ttctaaagat agtggtattc ctatttattc tgagatcttt acagattcaa
    11521 ttgctaaaaa aggtaaacct ggcgatagtt attatgctat gatgaaatgg aaccttgaca
    11581 aaatttctga aggtctagca aaataagata acaataaagc acagcgattt tagtgttgtg
    11641 ctttgtttta tttatgtaaa ggagacaaaa tgattacaac taacaatctt tgtgtgacct
    11701 acgatggcaa cagtaatgct ttagaagcga ttaatgtgac aattgaaggt ccttctatcg
    11761 ttggtattat tggtcctaat ggagctggaa aatcaacctt tatgaaagct attttaaatc
    11821 ttattgatta tcaaggtcac gtaacggttg atggcaaaga tggtcgtaag cttgggcata
    11881 cagtagctta cgtagaacaa cgaagcatga ttgactataa ttttcctatt actgttaaag
    11941 aatgtgtggc gctagggact tatagtaaat taggcctttt tcgccgagta ggaaaaaaac
    12001 aatttgagca ggttgataag gttctcaaac aagttggttt agaagacttt ggccatcgac
    12061 cgataaaatc cttatctggt ggtcagtttc agcggatgtt ggttgcgcgt tgcttgattc
    12121 aagaatcaga ctatattttc ttagatgaac cgtttgttgg cattgactcg gtcagcgaaa
    12181 aaatcattgt agatcttttg aaagagctca aaatggctgg caaaacgatt ttaattgtac
    12241 atcatgattt gagcaaagta gagcattatt ttgacaaact catgatttta aataaacatt
    12301 tggtggctta tggcaacgtt tgtgaggtat ttactgttga taccttgtca aaagcctatg
    12361 gcaatcattt gattttagga aaggagatgg tgtaaatgtc tatgaaattt tttgagggct
    12421 tgatgtcata ccacttttta caaaatgcac tgataacggc tgtagtcatt ggtatcgtct
    12481 caggtgctgt agggtgtttt attatcctta ggtcaatgtc tcttatgggt gatgccatct
    12541 cacacgctgt tttaccaggg gttgctttgt catttatttt aggagtcaat ttttttattg
    12601 gagcgattat ttttgggtta ttagcttctg ttattattac ttatatcaag gaaaactctg
    12661 tcattaaagg agatacggct attggtatca cctttagctc ttttttggca ctgggagtta
    12721 tcttgatagg ggtagccaat agttcgacgg acttatttca tattttgttt gggaatattt
    12781 tagctgtgca agatagcgat aagtggatta ctattggtgt ttcgattttt gttttagtgg
    12841 ttattagtct ctttttcaaa gaactattat taacatcatt tgatcctatc ttagccaaat
    12901 caatgggtgt gaaggtcaat gcgtatcatt atttgttgat ggtcctatta actttagtgg
    12961 ctgttacggc gatgcaaagc gtgggtacta tcttgattgt tgccttactg attacgccag
    13021 cagcgacagc ttatttatat gctaatagct taaaagtaat gttagtgatg tcatctttac
    13081 taggtgcttt agcatcggtt ttagggcttt atttgggcta tacctttaat gttgccgcag
    13141 ggtcaagtat cgtactgact tctgcgatga tgtttttgat cagtttcttt gtttcaccca
    13201 agcaaggcta cctcaaaaga tggatgcaaa aaaaagaaaa aacaccttaa acttttcgct
    13261 ttaaacacct tagtggtttg caaatagtgg caaataacat caaagggtgt tttttatatg
    13321 tcatcaaaag accataaaaa accttatcaa accaaggtat ttagcctatt atctgataag
    13381 gttttttaag catgatttag tttttaaagc gataatcttt gacaatgtca attgaggtaa
    13441 tcgtaatgtc ttgttttggt ttatcatttt ggttgataga agtcgcggca attttatcga
    13501 caacatccat accatcaatg acttgaccaa atacagtata accgccatct aagcttggat
    13561 tgccgccatg ctcataggca gagatgatag gttttgggta gttggtactt gataatccct
    13621 tgctttgatt tttcttgttt tggttgatat aaaattggct accgttagta ttagcgccag
    13681 catttgccat ggcaagagca ccgcgaatat gatataaaaa tggagagatt tcgttgacaa
    13741 agccattgcc agcatctttt ttaggatcct tgcccttcca aatcgattca ccacctgtgc
    13801 catctccttt ggggtcacct gattgaatca taaagtcgtt gatcacacga tggaaggtaa
    13861 ggttatcata gtagcctttt ttagcatggg tgaggaagtt ctcaacagct aagggagcat
    13921 attttgggaa taacttaagg gtgatgtccc cttgacttgt cctcattacg accttagctt
    13981 cttctttgcc gacttctttg gttagttgtg gaaattggct agcatcagct ttgagagctt
    14041 tttgaatgct ttcttcatag gctttagaag ctgcttcgct ttcttctttg gcagtttttt
    14101 catcaatgta tttatcgcct tttatagcgc ggtcaacaga ttcacaagca ctcaaactaa
    14161 gtaaactaat ggctactaaa ctgagagata ataatttttt catgtctact cctaagaatg
    14221 attttcttta ttttatcata aaatgcaaca gaaaatagta gtaaagagtt aaaaacgcct
    14281 atttttgtgt tttatgatct aaccttggtt aaaaggctgc tacgactgtt attatagcac
    14341 aagatgctat agaaaaactt aaaacacctt ggttttttag agctatttcc taatagttaa
    14401 aaaatggtat aatagtgtta tggttaaaag aaatcaaaga agaaaaagtg cgcctaagaa
    14461 gtgtctgaca aaagcagaag tggaaaagca acgtgccatt aagaggatga ttttatctgt
    14521 tttaatggct ttgttgctta tttttgcgat gcttcgcttg ggcgtctttg gcgtgacaac
    14581 ttataatatg attcgttttt tggtgggtag tttagcctat ccgtttatgt ttgcctggtt
    14641 aatttactta ttttgtttta aatggttacg gcaaaaagat ggaatgattg cgggagttgt
    14701 tattgccttt ttgggattgc tagttgagtg gcacgccttc ttatttgcga tgcctaggat
    14761 gcttgatcaa gatatttttc taggcactgc tcggctaatt acaagagatt tactagccct
    14821 gcgcgtgact gaatttgttg gtggaggaat gcttggtgca ttactttata aacccatagc
    14881 ctttttattt tcgaatatcg gctcttactt tattggtttt ttgtttatct tattaggatt
    14941 atttctaatg acgccttggg atatctatga tgtgagtcac tttgtgaaag aggctgttga
    15001 taaactagca gtggcttatc aggaaaataa agaaaagcgg ttcatcaaac gagaagagca
    15061 ccgcttacaa gctgaaaaag aggctttaga gaaacaagca caggaggaag aaaaacgtct
    15121 agcagaattg acggttgatc ctgagacggg agagattgtt gaggatagtc agtctcaagt
    15181 cagctatgat ttggctgagg atatgccaaa agagccagag attcttgctt atgatagtca
    15241 cctcaaagac gatgaggcaa gcttatttga ccaagaagac ttggcttacg ctcatgagga
    15301 aattggggct tatgactctt tatctgcttt agcatcttct gaagatgaga tggatatgga
    15361 tgaacctgtt gatgttgatt tcacgccaaa aacccatctt ctttataaac ttccaacgat
    15421 agatttattt gcaccagata agcccaaaaa ccagtccaaa gagaaaaact tagtccgcaa
    15481 aaatatcaag gtgcttgaag ataccttcca aagttttggg attgatgtca aggtagagcg
    15541 agcagaaatc gggccttccg tcaccaaata tgaaatcaag ccagctgtcg gggtccgagt
    15601 taatcgtatt tcaaatcttg ctgatgattt ggcacttgct ctggctgcta aagatgttcg
    15661 tatagaagca ccaattcctg gaaaatcctt aataggtatt gaagttccta actcagaaat
    15721 tgcaacggtt tctttccgcg aactttggga acaatctgat gccaatcctg aaaacctttt
    15781 agaagtacca ctaggtaaag ctgttaatgg taacgctcgc agttttaact tagctagaat
    15841 gccgcatctt ttggtagctg gttcaactgg ttcaggtaaa tcggttgctg ttaatggcat
    15901 tatttcaagt attttgatga aggcacgtcc agaccaagtg aaatttttga tggtcgaccc
    15961 taagatggtt gagttgtctg tttataatga tattccccat ttgctgattc cagtagtgac
    16021 caatcctcgt aaagccagca aggcgttgca aaaagtggtt gatgagatgg aaaatcgcta
    16081 tgaactcttt agtaaagtcg gggttcgaaa tattgctggt tacaatgcaa aagttgagga
    16141 ttacaatagg caatctgagc aaaaacaaat gcctttacca ttgattgtgg ttattgtgga
    16201 tgagttagct gatttgatga tggtggctag taaagaagtg gaagacgcta ttatccgtct
    16261 ggggcaaaag gcgcgtgcgg caggtattca catgattctt gcaacccaaa gaccatctgt
    16321 ggatgttatt tctggactga ttaaggccaa tgtgccatcc cgtattgcat ttgccgtatc
    16381 ttcaggaaca gatagtcgaa cgattctgga tgaaaatggc gctgaaaagc tcttaggacg
    16441 tggagatatg ctctttaagc cgattgatga aaatcaccct gttcgtctgc aagggtcttt
    16501 catctcagat gatgatgtgg agcgtattgt caactttatc aaagaccaag ctgaagcgga
    16561 ttatgatgat gcctttgatc ctggtgaagt tagcgacaat gatcctggtt tttctggaaa
    16621 tggcggagct gctgaaggtg atcctctttt tgaagaagca aaagccttgg tcttagaaac
    16681 acaaaaagct agtgcctcaa tgattcaaag gcgtttgtcg gttggattta atcgtgccac
    16741 gcgcttgatg gatgaattgg aagaagcagg tgttatcggt ccagcagaag gaaccaaacc
    16801 tcgtaaggta ttgcagacaa actaaaagaa cctgacgtct catgatgaga tgtcaggttc
    16861 tttttttgtg atgtcaagcc agttgatgag atgcctatgc taatgtaaaa ataaagataa
    16921 ctagaatcag gtagaaaata acggtgacta aaaagccaac tcgccaaaat agcttcataa
    16981 agcgatggta agaaaacgac tgagttcgga tgactagcag tagtgaaatg acgatggcta
    17041 gcagcgacat gataataagg taataaggca gtaaattatg ggtgaaaaat tttcctgaga
    17101 caagcacaat ttctatcgca aaaagtaaaa gtgcgacatc tggaaattta aatccctttt
    17161 tattaagttt aaataaaggg gttacaatca tggcaaaaag aggcgttact atcaagaaag
    17221 ctaaggcagt taatttatag atcatcatag gtctatttta tactcaaatc ccttacttgt
    17281 aaactgatac ttacctgttg tgatagaaaa tgatctttga gagggaaagc gcctacttca
    17341 atttaaacat tacaaaaggc atcagctatt ttaacaaaaa gacttgattt attttggcaa
    17401 taagctataa tggtagggta tgcaatattg catatctgtg ggaggtaaaa atctcaatta
    17461 ccgccaaaac cacaacagga ggatttttaa aatggctaaa aaagtcgaaa aacttgtaaa
    17521 acttcaaatc cctgctggta aagctacacc agctccacca gttggaccag ctcttggtca
    17581 agcaggtatc aacatcatgg gcttcactaa agaatttaac gctcgtacag ctgatcaagc
    17641 tggtatgatc atcccagttg ttatctcagt ttatgaagac aaatcatttg atttcatcac
    17701 taaaacacca ccagctgctg ttcttttgaa aaaagctgca ggtgttgaaa aaggatcagg
    17761 tacacctaac actactaagg ttgcgacagt tactcgtgca caagtacaag aaattgctga
    17821 aactaagatg ccagatttga acgctgcaaa cattgaagct gcaatgcgta tgatcgaagg
    17881 tactgctcgt tctatgggat tcactgttac tgactagtca gtcaagaacc cctattaccc
    17941 gcacgacttc atcataagag atgagtgacg tgggagattt taaatcaaat cgatatgacc
    18001 acattacaag gagaatttta aaatggctaa aaaaagcaaa caaatgcgtg ctgcacttga
    18061 aaaagtagat agcacaaaag cgtacagtgt agaagaagct gtagcattag taaaagaaac
    18121 gaacttcgca aaatttgatg cgtctgttga agttgcttac aacttgaaca ttgacgttcg
    18181 taaagcagac caacaaatcc gtggcgcaat ggtattgcca aacggaactg gtaaaacaca
    18241 acgtgttctt gtttttgcac gtggtgcaaa agctgaagaa gcaaaagcag ctggtgcaga
    18301 cttcgtaggt gaagacgacc ttgttgcaaa aatcaatggt ggatggcttg actttgacgt
    18361 tgttatcgca acgccagaca tgatggctat cgtaggtcgt cttggacgtg tccttggacc
    18421 tcgtaacttg atgccaaacc ctaaaactgg tacagtaacg atggatgttg ctaaagccgt
    18481 tgaagagtct aaaggtggta aaatcactta ccgtgctgac aaagcaggta acgtacaagc
    18541 tcttattggt aaagtttcat ttgatgctga caaattggtt gaaaacttca aagccttcca
    18601 cgatgtaatg gctaaagcta aacctgcaac agctaaagga acttacatgg caaacgtctc
    18661 aatcacatca acacaaggtg ttggtatcaa ggttgatcct aactcacttt aagaaaaaaa
    18721 ttagggcatc gcctaatcat agcaaaaaga caaatttctg aagacaaatc ttcactagag
    18781 gtttgtcttt tttatggttt aaaatggaat gcaaacaaaa aagtgtaggc gataatacca
    18841 tgaaaggagt tgattctctt aatgagatgt ggtgttaaca catcatgagc tcacaaaaaa
    18901 tgataaagga agtatcggtg gtgttaaaaa agggatttta attgacattt attagcctct
    18961 aaaatgtcct ttccctattc ataattgagg gtttgtggta aaatagtaag gacaaactaa
    19021 ggagaacata gtagtggaac ctaaatatca acgtatatta attaagttat ctggtgaagc
    19081 attagcaggt gaaaaaggag ttggcattga cattccaact gttcaggcta ttgcaaaaga
    19141 aattgccgaa gttcatgtgt cgggagtaca gatcgctctt gttattggtg gaggcaatct
    19201 ctggcgtgga gagcctgcag cagacgctgg aatggaccgt gttcaggctg attacactgg
    19261 catgcttgga acggtgatga atgccctagt catggctgat agtcttcaac attatggtgt
    19321 tgatacccgt gtgcaaacag ctattcctat gcaaaatgta gcagaacctt atattcgcgg
    19381 acgtgccttg cgccatctag aaaaaaatcg gattgttgtt tttggagctg gtattggctc
    19441 gccttatttt tcaacagaca ccaccgcggc actgcgtgct gctgagattg aagcagatgc
    19501 tattttaatg gcaaaaaatg gtgtggatgg ggtctataat gctgatccta aaaaagatgc
    19561 taatgcggtt aaatttgatg aattaacaca tggtgaagtg atcaaacgtg gtctcaaaat
    19621 catggatgca acggcatcga ccttatcgat ggacaacgat attgatctgg tggttttcaa
    19681 tatgaatgaa gctggcaata ttcaacgtgt tgtctttgga gaacatattg gaactactgt
    19741 atcaaataaa gtttgtgact aaaagttaga aaagaaagag agtaattatc atggcaaatg
    19801 caattattga aactgcaaaa gaacgttttg cacaatccca tcagtcttta tcacgtgaat
    19861 atgccagcat tcgtgcgggt cgtgccaacg ctagtctttt agaccgtatc caagttgatt
    19921 attacggggc accgacgcca ttaaatcaat tggcttcaat cactgtgcca gaagcgcgtg
    19981 tcttattgat ctcgcctttt gataaatctt ctatcaaaga tatcgagcgc gctcttaacg
    20041 catcagattt aggcattaca cctgctaacg atggctctgt cattcgtttg gttattccag
    20101 ctttgaccga agaaacacgt aaagaattag ccaaagaagt gaaaaaagtt ggtgaaaatg
    20161 ctaaaattgc aatccgtaat atccgccgtg atgctatgga tgatgctaaa aagcaagaaa
    20221 aagcaaaaga aatcactgaa gatgagttaa aaacacttga gaaagatatc caaaaagtaa
    20281 ccgatgatgc tatcaaagaa attgaccgca tgacggctga aaaagaaaaa gaattgctct
    20341 cagtgtaaca ggatttctac gaaaaataga cgactataga cacaaaaaga taggtgacca
    20401 tttcagaagc gtttggtttt gatttgtccc tattttttta aagaaagaaa aaagatatga
    20461 atgatttact agcaacagtc attactgggc ttattaaaga agaaaacgct aatgactatt
    20521 ttatccaaaa agaggggttt acctttacct tgtctaaagc agaaggagaa cgccagattg
    20581 gtgatatggt cacaggcttt gcctatacag acataaagca aaaggcacgt ctcacaacca
    20641 aagagatccg ttcaacacgg acaagttatg gctgggggga agtgacagag gttcgtcgtg
    20701 acctaggcgt ttttgtcgat acaggaattc caaataaaga aattgtggtt tctttggacg
    20761 tgttgcctga aatgaaagaa ctatggccta aaaaagggga taaactttac attcgtttgg
    20821 atgtggataa aaaagaccgt atttggggac ttccagcaga gcctgaggtc tttcaaaaga
    20881 tggctagccc tgcatacaat aatatgcaaa atcaacattg gccagcaatt gtttaccgcc
    20941 taaaattaac aggaacgttt gtttacctgc ctgaaaacaa catgttaggc tttattcacc
    21001 caagtgaacg ctacgcagaa ccgcgcttag gtcaggtttt agatgcccgt gtgattgggt
    21061 ttagagaggt cgatcgtacc ctaaacttgt cgttgaaacc acgttctttt gagatgttgg
    21121 agaatgacgc tcagatgatt gtcacttatc ttgaagccaa tggtggcttc atgaccttaa
    21181 atgacaaatc aagtccagaa gagattaaag caacctttgg tatttcaaaa ggccagttta
    21241 aaaaagcctt gggtggattg atgaaagcta aacgcatcaa acaagacgcc acaggaactg
    21301 aattaatagg ataaaaatgc tttgccactg gcaaagtttt ttgttacaat gaagaccatt
    21361 aagtgaagaa aaaggggata tagagaatgg aaagagccat ttttgcagga ggttgtttct
    21421 ggtgtatggt gcaacctttt gaggagcaag caggtatttt gtcagtcaga agtggctaca
    21481 caggtgggca tctcccaaat cctagctatg aacaagtttg tgccaaaaca acaggacata
    21541 cagaagctgt tgaaattatt tttgaccctg aagagatcag ctataaggaa ttagttgaat
    21601 tgtattgggt gcagacggat ccaacagacg cctttgggca atttgaagat cgtggggaca
    21661 actaccgtcc tgtcatttac tacacaacag aaagacaaaa agaaattgca gagcaatcaa
    21721 aagcaaactt gcaagcttca ggacgttttg accaacccat tgtcaccact attgaaccag
    21781 cagagccatt ttatctcgca gaggattatc accaaggatt ttataaaaag aatcccaggc
    21841 ggtatgcaca aagcagtgct attcgtcatc agtttttaga ggagaattgg tcatgagaaa
    21901 atcattttac agctggctga tgacacaacg taaccctaaa tcaaatgcac ctgtggcgat
    21961 tttagcagat ttggtgtttg atgacaccac ttttccaaag cataccaatg attttgaact
    22021 cattagtcgg tatctagaag atcaagctag tttttccttt aatttaggtc aatttgatga
    22081 aatctgggaa gattatctag cacattaata cgtggataag aaagagtcat atcgactctt
    22141 ttgaagggtt atcacgttat tattacaaaa tgacatcact ttctttcaaa aaaatgacaa
    22201 gctacttttt ttcttaaaaa tatgctatga tatagcttag aaaaaaataa aaaggaagca
    22261 gagatgatta ttactaaaaa gagtttattt gtgacaagtg tcgctttgtc gttagcacct
    22321 ttggcgacag cgcaggcaca agagtggaca ccacgatcgg ttacacaaat caagtctgaa
    22381 ctcgtcctag ttgataatgt ttttacttat acagtaaaat acggtgacac tttaagcaca
    22441 attgctgaag caatggggat tgatgtgcat gtcttaggag atattaatca tattgctaat
    22501 attgacttaa tttttccaga cacgatccta acagcaaact acaaccaaca cggtcaggca
    22561 acgactttga cggttcaagc gcctgcttct agtccagcta gcgttagtca tgtacctagc
    22621 agtgagccat taccccaagc atctgccacc tctcaatcga ctattcctat ggcaccatct
    22681 gcgacaccat ctgatgtccc aacgacacca ttagcatctg caaagccaga tagttttgtg
    22741 acagcgtcat ctgagctcac atcatcaacg aatgatgttt cgactgagtt gtctagcgaa
    22801 tcacaaaagc agccagaagt accacaagaa gcagttccaa ctcctaaagc agctgaaacg
    22861 actgaagtcg aacctaagac agacatctca gaagattcaa cttcagctaa taggcctgta
    22921 cctaacgaga gtgcttcaga agaagcttct tctgcggccc cagcacaagc tccagcagaa
    22981 aaagaagaaa cctctcagat gttaactgcg ccagcagcac aaaaagctgt agctgacacc
    23041 acaagtgttg caacctcaaa cggcctttct tacgctccaa accatgccta caatccaatg
    23101 aatgcagggc ttcaaccaca aacagcagcc ttcaaagaag aagtggcttc tgcctttggt
    23161 attacgtcat ttagtggtta ccgtccagga gatccaggag atcatggtaa aggattagcc
    23221 attgacttta tggtaccggt tagctctacg cttggtgatc aagttgctca atatgccatt
    23281 gaccatatgg cagagcgtgg tatttcatac gttatttgga aacagcgatt ctatgcgcca
    23341 tttgcaagta tttacggacc agcctacaca tggaacccca tgccagatcg cggcagtatt
    23401 acagaaaacc attatgatca tgttcatgtc tcctttaatg cttaattcat gctatcaaaa
    23461 aaactcacgt ttaggcgtga gttttttgac aattctgaca gagtaagccc tataagaggc
    23521 tgagatacgc ttttaatacc tgttgttttt ctcgcccttt agacagtttt aacgttatgt
    23581 ctaaaagaga tggtttttat tagcgcaaat atgggtttag gggtaaaata tagataatta
    23641 acctgatatg atcatgataa aggtagaaga atacctatta tttcgtgaca actgagcaat
    23701 ctgcccttac ctttggagtg atgatatcac tttgcttgac tattcaagga ggtcttgaaa
    23761 tgtattatac tagtggtaat tacgaagctt ttgcgacacc tcgaaaacct gaaggggtag
    23821 atcagaaatc ggcttatatt gttggtactg gtttagctgg tttagcagca gctgttttcc
    23881 ttattcgcga tgggcatatg gctggggaac gcattcatct gtttgaggaa ttgcctttag
    23941 caggtggttc tttagatggt attgaaaagc ctcatcttgg ttttgtgacc cgtggtggtc
    24001 gtgagatgga aaatcatttt gagtgtatgt gggacatgta tcggtctatt ccctcactgg
    24061 aaattcctgg tgcgtcttat ttggatgaat tttattggtt ggataaggat gatcctaact
    24121 catccaactg tcgtttgatt cacaagagag gaaatcgtgt ggatgatgac ggccagtata
    24181 cgctcggtaa acagtcaaaa gaattagtcc atttaatcat gaagacagaa gaatctctag
    24241 gagatcaaac cattgaagag ttcttctcag aagatttctt taagagtaat ttttggatct
    24301 attgggcaac catgtttgct tttgaaaaat ggcattctgc tgtagaaatg cggcgctatg
    24361 cgatgaggtt tatccaccat attgatggtt tgccagattt cacctctctc aagttcaaca
    24421 aatataacca atatgattct atggtcaaac cgattattgc ttacctagaa tcacacgacg
    24481 ttgacatcca atttgacaca aaagtcactg atattcaggt ggaacaaaca gctggtaaaa
    24541 aggtagcaaa aaccatccat atgacggtgt ctggggaggc taaggcgatt gagctaacac
    24601 ctgatgattt ggtttttgtg accaatggtt ctattactga aagtagcaca tacggtagtc
    24661 atcacgaagt ggctaagcca accaaagcgt taggtggttc ttggaattta tgggaaaatc
    24721 tagctgctca atcagatgat tttggtcatc ctaaagtgtt ttaccaggac ttgcctgctg
    24781 aaagctggtt tgtgtctgcc acagcaacca taaaacaccc agctatcgag ccttatattg
    24841 aacgtttgac ccaccgtgat ttgcacgatg gcaaagtgaa cactggcggc atcatcacta
    24901 ttacagattc taactggatg atgagctttg ccattcaccg tcaacctcat tttaaagaac
    24961 aaaaagataa tgaaaccact gtctggattt acggtcttta ttccaatagt gagggcaatt
    25021 acgtccacaa gaaaattgag gagtgtacag gtcaagaaat cacagaagaa tggttgtacc
    25081 accttggggt acctgttgat aaaatcaagg acttagcgag tcaggactat atcaatacag
    25141 ttcctgttta catgccttat attacgagtt actttatgcc acgtgtcaaa ggagaccgtc
    25201 cgaaagttat cccagatggt tcagtcaact tggcctttat tggtaacttt gcggaatctc
    25261 catctcgaga tacggtcttt acgactgagt attctattcg tactgccatg gaagcagtgt
    25321 atagcttctt gaatgtggaa cgaggcattc cagaagtctt taattcagcc tatgatattc
    25381 gtgaattgct caaagccttt tattacctta atgataaaaa ggcaatcaag gatatggatt
    25441 tgccaattcc tgcactgatt gagaaaatcg gacataaaaa aatcaaggat acctttatcg
    25501 aagaattgct caaagatgct aatcttatgt aacatgcctt agagtagtta aagctgataa
    25561 gtttaggctt atcagctttt ttgatgattt atagaaaagt gataaaggaa aagtctgacg
    25621 tgaacgtcac ataagaattt aaaatttgtt ataatgtaag caataaagat gaaagaaaga
    25681 gggactctat ttgcaagagt attctattga cattacctta acccatcctg atgatgtttt
    25741 agctcttttt ggtagcaacg agcgtcattt gaagctaata gaagctcatt taggagttat
    25801 tgttcacgca agaaccgagc gagtccaagt gattggtgat gatgaagaag ctgtggaatt
    25861 ggctcgattg accatcaaag ccctactggt tctagtgggt cgtggcatgg tggtcaacac
    25921 atcagatgtg gtgacagctt tatctatggc agaatctcat cagattgacc aatttatggc
    25981 gctttatgaa gaagaaatca ttaaagataa ctatggtaag gcaatacgag tcaaaacatt
    26041 aggtcaaaaa acctatgttg atagtgtcaa acgtcatgat gtggtttttg gtgtaggacc
    26101 tgcagggaca gggaaaacct tcttagctgt tacgcttgca gtcactgctc tgaaaagagg
    26161 acaggtaaag cgtattattt tgacgcgtcc tgcggtagaa gctggtgaaa gcctaggttt
    26221 tttaccaggc gatttgaaag aaaaggtgga tccttacctc agacctgttt acgatgccct
    26281 ttatcacatt ttaggcaagg aacaaaccac acgcctgatg gaacgagatg tgattgagat
    26341 tgctccgtta gcttatatgc ggggtcgtac tctggatgac gcttttgtta tcttagatga
    26401 ggctcaaaac acgaccatca tgcaaatgaa gatgtttttg acccgtcttg gttttaattc
    26461 taaaatgatt gtcaatggag atacgagtca gattgacctc cctcgaaatg tcaagtctgg
    26521 tttgattgat gctactcaga aattacaagg cattaaacag attgattttg tctatttctc
    26581 tgctaaggat gttgtccgac atcctgttgt ggctgatatt atcaaggctt atgaaacgtc
    26641 ctcagaggag atgaaggact tactaaaacc taaggctagt gacgttgaag ctgattacca
    26701 gccaggcttg acaaaatacc ctgtgattgg tcaagagcac taagattttt acgatgatgt
    26761 ttaatatggc ctttttaagg aggattctat ggatgattta accaaagcta ttatggcaga
    26821 tgaggctaat ctctcctata ccgaaagagg tatttttcct ttatatgatg ctcctcaaac
    26881 agctaggctt attattgttg gccaagctcc tggaatagta gctcaagaaa ccaaacttta
    26941 ctggaatgat cgtagtggca tccgcttacg agactggcta ggtgtggaca atgacacctt
    27001 ttaccactca ggattgtttg gcattatccc tatggatttc tattatcctg gtaagggaaa
    27061 atccggtgac ttaccaccta gagaaggttt tgcggctaag tggcacccgc cattaagagc
    27121 attgatgcca gaagttgaat tgacgatttt ggttggtcgc tatgctcagg acttttacct
    27181 tggtaacaag gcttacaaaa ccttaactga aacagtccgt cactttgagg attacttgcc
    27241 agactatttt ccacttgttc atccttctcc aagaaaccaa ttgtggttag ctaagaaccc
    27301 ctggtttgag caggatttat tacctatctt gcaaaaacgt gtggaggcaa ttctaaccaa
    27361 ataggccctg atttacctag cattcccatt ttttaggggc ttgtggtata atgggtaaat
    27421 aacaaatgct gattgaaagg ataagggaaa ttatctgtca gataataatg ggtgcccaac
    27481 ataccaaatg aggtgggcag cccttcccta acaattaacc ttatgtatat cgagatgatt
    27541 gacgaaacgg gacaagtttc gcaagagatt atggagcaaa cacttgattt gctcaatttt
    27601 gctgctcaaa agacgggcaa agaagaaaaa gaaatgtctg tgacctttgt gaccaatgaa
    27661 agaagtcatg aactcaactt agaataccga gatactgatc gtccaacaga tgtgatttct
    27721 ctagaatata agccagaaac gcctatttta tttagtcaag aggattttgc tgctgatcct
    27781 agtttggcag agatgatggc agagtttgat gcttatattg gcgaattgtt tatttctatt
    27841 gacaaggcgc gtgagcagtc ccaagaatat gggcactctt ttgagcgtga aatgggattt
    27901 ttagctgttc atggattctt acatattaat gggtatgatc actacaccct tgaagaagaa
    27961 aaagagatgt ttaccttaca ggaagagatt ttgactgctt atggccttac acgacaataa
    28021 taccaccaaa cggaaatgga aaaacagaac cattacctca agcctggagt ttgccttgac
    28081 aggggttttc acagctttta aggaagaacg caatctcaga agtcaccttt tgtctgcctg
    28141 tttagcttgt gttgctggtt tattttttag catttcagcc atcgaatggt tatttttact
    28201 tctagccatt tttttggtca ttaccttaga aattgtcaat tctgctattg aaaatgtagt
    28261 tgatttggct agtgactatc acttttctat gctagctaaa aatgccaagg atatggcagc
    28321 aggtgctgtt ttgatgattt ctggctatgc tgtcttgaca ggacttatta tttttattcc
    28381 taaaatttgg aatattttcg ttcattaaaa agaaaggaat gggctatcct atgtgatgat
    28441 ggcataggag aataaggaaa ggacaagacg gttaagctta gtgacaagct tgatctgaag
    28501 ccctaccgtt aaggcccgta attatctatg tttaaatcag gctttgtagc cattcttggt
    28561 cgtccaaatg ttggcaaatc caccttttta aatcacgtca tggggcaaaa aattgccatc
    28621 atgagtgaca aggctcagac cacccgtaat aaaattatgg gcatttacac cacagaaaca
    28681 gaacaaattg tctttattga tacgccaggt attcataaac caaagaccgc ccttggtgat
    28741 tttatggttg aatctgctta tagtacttta cgtgaagttg agacggttct tttcatggtt
    28801 cctgccgatg aaaagcgtgg taagggtgac gacatgatta tcgagcggct aaaagcagct
    28861 aagattcctg ttattttagt tatcaacaaa attgataagg ttcatccaga ccaacttttg
    28921 gaacagattg atgacttccg tagccaaatg gattttaaag aagtggtccc aatttctgcc
    28981 ctagaaggta ataatgtgcc aaccctaatc aagttattaa ctgataatct tgaagaaggt
    29041 ttccaatatt ttccagaaga tcagattact gatcatccag aacgtttcct tgtgtcagaa
    29101 atggttcgtg agaaggtact gcatttgacg caacaagagg ttccacattc cgtagctgtt
    29161 gttgtcgaat ccatgaagcg agatgaagag actgataaag tgcatatccg tgcaaccatt
    29221 atggtggagc gcgatagcca aaaaggcatc atcattggaa aacaaggagc catgctcaaa
    29281 aaaatcggaa aaatggcgcg ccgtgatatt gaactcatgt taggtgacaa ggtctactta
    29341 gaaacgtggg tcaaagtcaa gaaaaactgg cgggataaaa agcttgattt agctgatttt
    29401 ggctataatg agaaagaata ctaacatagt aaagcgagat catatgccac aagactacat
    29461 ttcttatatc cgctccaaag ttgggcatga taagattatt cttaactttg ctggtgggat
    29521 tttgaccaat gatgacggca aggtgctgat gcagttgcgt ggtgataaga aaacctgggc
    29581 tattcctggt ggtactatgg aactgggtga gagctcttta gagacttgca aacgtgagtt
    29641 cttagaggaa accggaattg aggttgaagc tgttcgctta ctgaatgttt acactcattt
    29701 tgaggaagtt tatcccaacg gtgatgctgt tcagactatt gtctttatct atgaactaac
    29761 ggctgtttct gatatggcta ttgataactt tcataatgag gaaacgctca agttacagtt
    29821 tttctctcat gaggagatag cagagttaga gagtgtctca gccaaacatc gcctgatgtt
    29881 agaggaatat tttagtgata gctttgctat ggggcattaa gaactctcta ggacagttgg
    29941 agtatttttg gagattggct tgttaaaaaa atttttgaaa gacgatgttt cgagagctta
    30001 tgctctcttt ttttgcttcc aatttttctg attaagtctg aataattttg ttaaatgtta
    30061 acaggatttt ctttccaatt gatactagca gacatcaaac atttgtttta gagttatgac
    30121 gaattgccac ctagccatgc atgacttagg caagttgaac ctaactgtgg caatagactt
    30181 ggaaggggtg acaagaatca tttcctaaaa gaattgatga agtgcctatc cattattcga
    30241 attgtaaagt tcgtctaagt aaagttgaat ttgatctcga atggacttta atgatttatg
    30301 acttgttgtg tcaataacac caaaaactcg ataatgattt ttaaatattt cctcgtaatt
    30361 aatatgattt ccgattagaa taatatgagc atgaggataa cgcatacgta tttcgtttag
    30421 aaggtcatat acttgctc
//
LOCUS       DATUYR010000017        30198 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.017, whole genome shotgun sequence.
ACCESSION   DATUYR010000017 DATUYR010000000
VERSION     DATUYR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.017"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..189)
                     /locus_tag="VCI33_000712"
     CDS             complement(<1..189)
                     /locus_tag="VCI33_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2831649.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFI"
     gene            1130..2326
                     /gene="speB"
                     /locus_tag="VCI33_000713"
     CDS             1130..2326
                     /gene="speB"
                     /locus_tag="VCI33_000713"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEP2831650.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSVGSSRVQRALKENFGYNQSVHQINRSDFSKQDWESQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            2328..2633
                     /locus_tag="VCI33_000714"
     CDS             2328..2633
                     /locus_tag="VCI33_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEP2831651.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMIEAYQTQINSLD"
     gene            3111..4040
                     /gene="prsA"
                     /locus_tag="VCI33_000715"
     CDS             3111..4040
                     /gene="prsA"
                     /locus_tag="VCI33_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP2831652.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(4316..4552)
                     /locus_tag="VCI33_000716"
     CDS             complement(4316..4552)
                     /locus_tag="VCI33_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831653.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPAAHGPCD
                     DIHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            4976..8056
                     /locus_tag="VCI33_000717"
     CDS             4976..8056
                     /locus_tag="VCI33_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP2831654.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSASSSTESSTTT
                     ANTGTGTASGMTATTPSATTDTGEAAGSGARSEANGASSVVSSEESQSSGTTPASPQA
                     QTAPAATSTSSASSSNEKTPKTATTTTSSTPVASTSNNSNKVTSTEAETQTMDVERYT
                     VDKENSKLNIKDGKTPKTRSSVNKDTKLIRNRDDKQRDIVDVTRTVKTNEDGTIDVTV
                     TVKPKQIDEGADVMALLDVSQKMTKENFDKAKEQIKKMVTTLTGEPTDGKENHNRRNS
                     VRLMTFYRKVNEPIELTAENVDKTLDEVWKKAKEDWDWGVDLQGAIHKAREIFNKEKE
                     KKSGKRQHIVLFSQGESTFSYDIKNKSDITKTKITEKVTTSSPLFPWLPIFNHTNRKA
                     EIIGDLEKVLDMAEKVGISLPSSLKLAVKALGLTNSAIGSILGKGLTEYLGLTEYSSD
                     NLDGGGFDYSKRVGEGYYYHSFSDRKYENTMPLEEAIRTALASNFPKLTDNWFFDILN
                     SFVNKDTVEKAKLDVIMKVLNSIFYKREYRYYNHNLSAIAEAKMAQQEGITFYSVDVT
                     DLNSASKRVRRQAAVRKGTKEEENKKNEEERNTKFDTYLKKMSEGNNFLSNVEERDKF
                     KDTLTELTIKDEFTDKVTVEKDSWSKSITDGLKNSNNNNVKHQQANTSTWSFFSSSKE
                     SLTWIISKEQLKEAFEKNGSLTFKYKLRVNKDKLLDKDKKRIKRDTPTENKTSVTADI
                     ISNTVDYKINNQEVKGNKLDAVKLTYTKETVPVPDVEGEVVPIPEKPLVEPMTPLYPA
                     IPNYPTPQLPKDEDLEISGGHGPIVDIVEDTGTGVEGGAQNGVVSTQENKDPIVDITE
                     DTQPGMSGSNDATVVEEDTAPKRPDVLVGGQSDPIDITEDTQPSVSGSNDATVVEEDT
                     VPKRPDILVGGQSDPIDITEDTQPGMSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPK
                     EQPSLSLPQAPVYKAAHHLPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
     gene            8236..10179
                     /locus_tag="VCI33_000718"
     CDS             8236..10179
                     /locus_tag="VCI33_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP2831655.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAISNSLEYQAQYERVKEE
                     LDALKEKGPKLGERSLYFRLVNKFLSWFGQVWDPLAIKETDYLLDLIGQVNYLVSNTE
                     FQAPEYKDAKNKLDLAVAGYDLYLAREKKRGDAPDYQVTQLTLEQLKTIKKKIREALE
                     TFAQEVKTIQQKNPDLFPHAYVLNAKSKDITDKAQPFLVNSYYDVFYKQFLFTNTNDG
                     YNYNKEADNLTSYQNLIKELLGLKAEIDQLTKGKTGDDRLEMLMWQSKFTLSDSRKKS
                     HLSAYYTQGEYVDKVVLSEEAPNLRTAKEKVKTLKGILHDYYVETNKPEKAKKYHVED
                     ETLPSQPEAPATPEAPAPVPAPVPVPGQKPAEDERSPQATEPANPSKEDSSTDASQGS
                     HDSENPATDSPSQPQAPDQGNHQSQVPNAKPQTDKTDTPNAPAIPQDTPKAPEAGGQS
                     GPAGNTEEKAPDSGPKESDQSSSKESPSVGEDTTPNQPDVLVGGQSEPIDITEDTITD
                     APPTVSGHNASTQPQSVVEDTAPQRPDVLVGGQSEPIDITQDTQPGMSGSNDATVINE
                     DTKPKRVFHFDNKEPQASEKAAEQKLAPHDSHTTPQASDDTAAPHLPASGDKPEKVFA
                     LAALTMVAAAGLLSKKRRNTDKN"
     gene            10321..10617
                     /locus_tag="VCI33_000719"
     CDS             10321..10617
                     /locus_tag="VCI33_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEP2831656.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADVLDLAPIMYKHM"
     gene            10766..11050
                     /locus_tag="VCI33_000720"
     CDS             10766..11050
                     /locus_tag="VCI33_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEP2831657.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            11484..12752
                     /locus_tag="VCI33_000721"
     CDS             11484..12752
                     /locus_tag="VCI33_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP2831658.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            12739..13413
                     /locus_tag="VCI33_000722"
     CDS             12739..13413
                     /locus_tag="VCI33_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831659.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            13426..14643
                     /locus_tag="VCI33_000723"
     CDS             13426..14643
                     /locus_tag="VCI33_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831660.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            14737..15390
                     /locus_tag="VCI33_000724"
     CDS             14737..15390
                     /locus_tag="VCI33_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2831661.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            15387..16775
                     /locus_tag="VCI33_000725"
     CDS             15387..16775
                     /locus_tag="VCI33_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP2831662.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLM
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            16877..18502
                     /locus_tag="VCI33_000726"
     CDS             16877..18502
                     /locus_tag="VCI33_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP2831663.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKSSHTHLTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATDKTPEPNRDAPKPIQPPLAAAPVFAPWRESDKDLSKLKPSSRSSAAYVR
                     HWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVRA
                     IVAIAMAESSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIANK
                     NQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMIKLDQWIDDHGSTPE
                     IPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVDR
                     YMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILISE
                     SNVMGLGTISYRTFTAEQARLLTYVVGDKLPRP"
     gene            18580..18831
                     /locus_tag="VCI33_000727"
     CDS             18580..18831
                     /locus_tag="VCI33_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831664.1"
                     /translation="MIKKVTTPSQKIKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
     gene            19507..21114
                     /locus_tag="VCI33_000728"
     CDS             19507..21114
                     /locus_tag="VCI33_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2831665.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPISGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQKEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIEK
                     QYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSPS
                     KSHSDKEVQKPEKPDNSVKQATSATSS"
     gene            21304..22353
                     /locus_tag="VCI33_000729"
     CDS             21304..22353
                     /locus_tag="VCI33_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP2831666.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLANTTDVKAETE
                     HVDVVLSAKEANKVFEERKALEKQARDLGDTINHMSQTISEQSRKIAALKSEAELKNQ
                     QALEALNNKNEQIAKLTNENAQLKEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAK
                     NAEIEALKQQDASKTEEIAKLQSEAATLENLLGSAKRELTDLQAKLDTANTEKEKLQS
                     QAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKALKEQLAKQAEELAKLKADK
                     ASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLPSTGEETTNPFFTAAAATV
                     IASAGVLALKRKEEN"
     gene            22578..23654
                     /locus_tag="VCI33_000730"
     CDS             22578..23654
                     /locus_tag="VCI33_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP2831667.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTEVKAAES
                     PKSTETSANGADKLADAYNTLLTEHEKLRDEYYTLIDAKEEEPRYKALRGENQDLREK
                     EGKYQDKIKKLEEKEKNLEKKSEDVERHYLKKLDQEHKEQEERQKNLEELERQSQREI
                     DKRYQEQLQKQQQLETEKQISEASRKSLSRDLEASRAAKKDLEAEHQKLKEEKQISDA
                     SRQGLSRDLEASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEKEKAELQARL
                     EAEAKALKEQLAKQAEELAKLKGNQTPNAKVAPQANRSRSAMTQQKRTLPSTGEAANP
                     FFTAAAATVMVSAGMLALKRKEEN"
     gene            23865..24797
                     /locus_tag="VCI33_000731"
                     /pseudo
     CDS             23865..24797
                     /locus_tag="VCI33_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            25132..28629
                     /gene="scpA/B"
                     /locus_tag="VCI33_000732"
     CDS             25132..28629
                     /gene="scpA/B"
                     /locus_tag="VCI33_000732"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEP2831668.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPATSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTDDHQDKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPIYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGGTVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSGQAPDKKPEAKPEQDGSGQTPNKKPETKPE
                     KDSSGQTPGKTPQKGQPSRALEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVM
                     TTFFLGLVAHIFKTKRTED"
     gene            28726..29982
                     /locus_tag="VCI33_000733"
     CDS             28726..29982
                     /locus_tag="VCI33_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEP2831669.1"
                     /translation="MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDSMQPI
                     SSTQGNRANTSKKWYDINEYGLIPVDISFEAAKKDFKEEVEKSLLSEEQQKTYNQKIE
                     AARNKDDLLLTYHSEYSKDIKKHSTSTEPVEASVQETQASVSDSMVTDDSTSVTADSP
                     EKTPSTETPAPSIPEVPEAPASSPESEESSVASSEETSSPETPVAPEAPALSEAPAQP
                     AESEESSVAAPSEETPSPETPAAPETPEEPAAPSPSPESEEPSVAASSEETPSPETPA
                     APETPEEPAAPAQPAESEESSVAATTSPSPSTPAESETQTPPAVTKDSDKPSSAAEKP
                     AASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAHESGKYLPSTGEKAQP
                     LFIATMTLMSLFGSLLVTKRQKETKK"
BASE COUNT        10825 a         6100 c         5929 g         7344 t
ORIGIN      
        1 aataaagaga aactcatcaa cattgacatt caagttgtct aagatataaa gtaacttttt
       61 cgaagaaatg tcaagattat ttttaataaa acgataatag gtttgcctag tgagataatc
      121 accacaaact tgtttaatcg aaatgttttt tgaatgccta atgaattcaa cggtttcacc
      181 aatttccata tgtcaagcct tcctagttga tgtcaaaaat acgttacgca tgtacctaat
      241 acgtaacaag ttgaatgttt cggatgatag tcgcttatga taggtgcata aggtcaatag
      301 ccagatgcga tagttttatc aactgtcata tgttaaacct ttctaatcta atagatgtta
      361 aaaatacgtt acgtgtgtac ctaatacgta acaaaataat gggttagcaa aataagcagc
      421 tatgatatag ccataaggtt aaaaggaggc gcctactatg tggttattgt tactattttt
      481 gtagttcctt ttgcaaaatg gtagtagata aaaatgagaa gtcaataaaa gcattgacaa
      541 acaaaagaat cattttgttt atattatcag tatttttaag tttcttgtca actgaaatga
      601 gcatctacta gccacaatag taactaaaag gctaattgtc agtaaaaatg agataaatca
      661 gccaggaaaa agcctacgag tcaactaatg gacaacccca aaaaagacaa aggactttca
      721 cttggtgttg tggaccctat cacgatcctt atcacagaat cagttattaa caaaatgaga
      781 acggtcccat atcatctaaa aaaactgtca agcacaacct attgtttgct agctgatctt
      841 gcctatcagt ctgcctagta gatacggcag aagataagag atcaaccaaa agatgataag
      901 tgcaacgact catagcgtct tttgaccaaa accaattaga ctcacatgag atgagaataa
      961 ttgggttggg ttgtcagtgt caactaaccg tgttattgtc tattaccatt catggtatca
     1021 gcgacatcgt atgataacca tgcgattcag ctaagtaagg aggtgtgtcc aatgtactgt
     1081 taaaagcaaa tgcagtagat taacttattt tgaaagaggt ataaaaaaaa tgaataaaaa
     1141 gaaattaggt atcagattat taagtctttt agcattaggt ggatttgttc ttgctaaccc
     1201 agtatttgcc gatcaaaact ttgctcgtaa cgaaaaagaa gcaaaagata gcgctatcac
     1261 atttatccaa aaatcagcag ctatcaaagc aggtgcacga agcgcagaag atattaagct
     1321 tgacaaagtt aacttaggtg gagaactttc tggctctaat atgtatgttt acaatatttc
     1381 tactggagga tttgttatcg tttcaggaga taaacgttct ccagaaattc taggatactc
     1441 taccagcgga tcatttgacg ctaacggtaa agaaaacatt gcttccttca tggaaagtta
     1501 tgtcgaacaa atcaaagaaa ataaaaaatt agacactact tatgctggta ccgctgagat
     1561 taaacaacca gttgttaaat ctctccttga ttcaaaaggc attcattaca atcaaggtaa
     1621 cccttacaac ctattgacac ctgttattga aaaagtaaaa ccaggtgaac aatcttttgt
     1681 aggtcaacat gcagctacag gatgtgttgc tactgcaact gctcaaatta tgaaatatca
     1741 taattaccct aacaaagggt tgaaagacta cacttacaca ctaagctcaa ataacccata
     1801 tttcaaccat cctaagaact tgtttgcagc tatctctact agacaataca actggaacaa
     1861 catcctacct acttatagcg gaagagaatc taacgttcaa aaaatggcga tttcagaatt
     1921 gatggctgat gttggtattt cagtagacat ggattatggt ccatctagtg gttctgtagg
     1981 tagctctcgt gttcaaagag ccttgaaaga aaactttggc tacaaccaat ctgttcacca
     2041 aatcaaccgt agtgacttta gcaaacaaga ttgggaatca caaattgaca aagaattatc
     2101 tcaaaaccaa ccagtatact accaaggtgt cggtaaagta ggcggacatg cctttgttat
     2161 cgatggtgct gacggacgta acttctacca tgttaactgg ggttggggtg gagtctctga
     2221 cggcttcttc cgtcttgacg cactaaaccc ttcagctctt ggtactggtg gcggcgcagg
     2281 cggcttcaac ggttaccaaa gtgctgttgt aggcatcaaa ccttagtatg gaaatgcatt
     2341 tcgttagaac agaacctgag gcacgccgca tagctgaaac cttttgtgcc gaaaacacac
     2401 aaacaaaaac ccctatgcgc gtgcagcagt taagctatcc atcagacaca gatcactcag
     2461 gtggtgagct atacatctat gctttgtctc ctgctggatt tatcatcgta tcaggagaca
     2521 ccagagcgca caccatttta ggctattctt ttgataataa cctagacctc aaccatgata
     2581 atgtcaggag tatgatagaa gcttaccaaa cacaaatcaa ctctttggat tgatcggtca
     2641 aagtagcttt tgcccccctc accactcgtt taagacgacc gtgtttctaa aataccacta
     2701 ccttttaagt ttcacgccgg cctaagcgag tgtttgtctc atggagcaga ctgtttccat
     2761 cgaaaagtca cccattgata aaggaggtca tcaacgccta ctaatgatga cttccctaag
     2821 atgccaaggt aaaggactga gttctgtaga gatgggactc agtcctttta cgatttttgg
     2881 gagttattct agccataccg tggcaagaaa acaacactta aaaatagcca acgtcagttt
     2941 ctgttcatac atgactacag ccaaacacca caacctaacc tatcctaaac acagtggctt
     3001 tctaacgaac agtcacaacg agcagcaaca ccaaaaagca agattaagct acgtttaaga
     3061 ctaatgatat aagataacag taattattaa tgagaaaaga ggctcaggac atgaaacaaa
     3121 tgaataaact cattacagga gtggtaacgc tggcgacagt cgtgacctta tcagcttgtc
     3181 aatcatcaca caacaacacc aaactcgtct cgatgaaagg agacaccatc actgtcagtg
     3241 acttctacaa tgagaccaaa aacacagaac tcgcacaaaa agccatgtta agcttggtga
     3301 ttagccgtgt atttgagaca caatatgcca acaaagtctc tgacaaagag gttgaaaaag
     3361 cctataaaca aaccgcagac caatacggta catcctttaa gacagtccta gcacaatcag
     3421 gcttaacgcc agaaacctat aaaaaacaaa ttcgcctcac aaaattagtc gaatatgccg
     3481 tcaaagaaca agccaaaaac gaaaccatct caaaaaaaga ctaccgtcag gcctatgacg
     3541 cttatacccc aaccatgacc gcagaaatca tgcagtttga aaaagaagag gatgccaaag
     3601 cagcccttga agccgtcaaa gctgaagggg cagactttgc agctattgcc aaagaaaaaa
     3661 ccattgcagc cgataaaaaa acaacctaca cgtttgactc aggtgaaaca accctaccag
     3721 cagaagtagt tagagctgca tcaggcctca aagaagggaa cagatcagaa atcatcacag
     3781 cgcttgatcc agccacctca aaacgcacct accatatcat caaagtcacc aaaaaagcaa
     3841 ctaaaaaagc agactggaaa gcgtaccaaa aacgcttgaa agacatcatc gtgactggca
     3901 aattaaaaga ccctgacttc caaaacaaag tcatcgctaa agctcttgat aaagcaaatg
     3961 tcaaaatcaa agacaaagca tttgccaata tcttagccca gtttgcaaaa cctaaccaaa
     4021 aacaacctgc ccaaaaatag agggttgcca gtttatttcc ctaaggttgc ttaaaaagac
     4081 agccctaacc atcactcccc ttttcaaaac accgataatg gaaacgtgtc ctatactgat
     4141 ggtctagggc aatgtcataa agctaaagac tctatccaaa taaggataga gtctttaata
     4201 tttaaaaacc agtcagcaaa aaaaattcac gctaagccta gctagaatga ggcttagcgt
     4261 gaatggtatc agtgatagtc cgttaacata gctgattggg agtcagaaac tctaactacc
     4321 caatctgact atacccccag taccagaggt gtctcatcgc agaagtagca atcagataat
     4381 cgtctgataa gctcttgtcc tcaagcaact gatgaatatc gtcgcacggg ccatgagctg
     4441 ccggtggctg atagctagta ggctcatcag ccaaaacttt tttggagtaa cgctttggtc
     4501 tcagacggcg ccagagggtt gagagagggg tgagaaaaaa acgatgggtc atcttggcct
     4561 ccttgtctaa tgatagtcac cttaacagac aaaagtgtca ataatgtcat tttaaggaga
     4621 aaaagattaa aaatcaaggt cacaaacttt cctttgttaa aaaaacgcga ccctaacaaa
     4681 gaatctaaat acctctcgca cttgagcttc tccttgcgca gctatcttgt gcactcagtt
     4741 gcctccaaga taatgataac gataccatca ttatcctata ggctatcgcg acaacccctt
     4801 ttttcgcctc aacgagtctg acacagctgt catgacagaa aaaagacctc tgcctatcta
     4861 agagaccatt aatttttctc tcactaaaaa actgatttta gaaacgaaga agcatggtgt
     4921 ataataaggt tcagaacaat tatgacataa taatgaaagt aaggttaacg aaacattgac
     4981 aaattgtaag tataaactta gaaagttatc tgtagggctc gtctccgtcg gaacgatgct
     5041 gatagccccg acagttttag gacaggaggt tagtgctagt agtagtacgg agagcagtac
     5101 cactacagct aatactggta ccggtacggc aagtgggatg actgccacta ctcctagtgc
     5161 tacgacagat actggtgaag cagctgggag cggagctagg agtgaagcta atggtgcatc
     5221 gtccgtagta tctagcgaag aaagtcagag ttcaggcact actccagcct caccccaagc
     5281 acagacagct ccagcagcaa cgtcaacatc atcggcttct tctagtaatg agaaaactcc
     5341 caagacagca actacaacta catcatcgac tccagtagca agtaccagta ataatagcaa
     5401 caaagtaact agtactgaag ctgaaacaca gacgatggac gtggaacggt atacagttga
     5461 taaggaaaat tcaaagctaa atattaaaga cggtaagact ccaaaaacta ggagtagtgt
     5521 taataaagac acaaaactta ttagaaaccg cgatgacaaa cagcgtgata tcgttgatgt
     5581 tactcggaca gttaaaacta acgaagatgg cactattgat gttaccgtaa cggttaaacc
     5641 gaagcaaatt gacgaaggcg ccgatgttat ggcccttcta gatgtctctc aaaagatgac
     5701 aaaagagaat tttgataagg ctaaagaaca aataaaaaaa atggttacga ctttaacagg
     5761 cgagccaact gatggtaagg aaaatcataa taggcgtaat tctgtacgtc taatgacttt
     5821 ttaccgtaag gttaatgagc ctatcgaatt gactgctgaa aatgtagaca aaacattaga
     5881 tgaagtttgg aaaaaagcta aagaagattg ggactggggc gttgacttac agggagctat
     5941 ccataaagct cgagagattt ttaataagga aaaagaaaaa aaatcgggta aacgccagca
     6001 tatcgtcttg ttctctcaag gcgaatcaac ctttagttat gatattaaaa ataaaagtga
     6061 tattacaaaa acaaaaataa ctgaaaaagt tacgacctct agcccactgt ttccttggct
     6121 ccctatcttt aatcacacta accgaaaggc agagattatt ggtgatttag aaaaagtact
     6181 agacatggcc gaaaaagtgg gaataagttt acctagtagt ctgaagttag cagtgaaagc
     6241 ccttggctta actaatagtg caataggttc tattttaggg aaaggtttga cagagtacct
     6301 tggtttgaca gaatatagtt cagataactt agatggagga gggtttgatt atagtaaacg
     6361 tgtaggggaa ggttactact accacagttt ttcagatagg aaatatgaaa atacaatgcc
     6421 ccttgaagaa gctatcagga cggccttagc atctaatttt cccaaactca cagataattg
     6481 gtttttcgat atcttaaata gttttgtcaa taaagataca gttgagaaag ctaaattaga
     6541 cgtaattatg aaggtactta atagtatttt ttacaaacgt gaatatcgct attacaacca
     6601 taacctgtca gcaatagccg aagctaaaat ggctcaacaa gagggcatta ccttctattc
     6661 cgttgatgtt actgatttaa actcagcttc taaaagagtg aggcgacaag cagcagtgcg
     6721 taagggcact aaggaagaag aaaacaaaaa aaatgaagaa gagcgtaata caaagtttga
     6781 cacctatctg aaaaagatgt ctgaaggtaa taatttctta agtaatgttg aagagagaga
     6841 taagttcaaa gatactttaa cagagctgac aattaaagac gaatttacgg ataaagttac
     6901 ggtcgagaag gattcctgga gtaaatctat aactgatgga cttaaaaatt caaataacaa
     6961 taatgttaaa catcaacagg caaacacatc gacatggagt ttcttcagtt catccaaaga
     7021 aagcctcacc tggattattt ccaaagagca gctcaaagaa gcctttgaga aaaatggttc
     7081 tctcactttt aaatacaaat tacgggtcaa taaagacaaa ctattagata aagataagaa
     7141 gagaataaaa cgtgatacac ctacggaaaa taagacttct gtaacggcag atatcatttc
     7201 aaatactgtt gactacaaaa ttaataatca agaagttaag ggtaacaaac ttgatgctgt
     7261 caagttgact tatactaaag agaccgttcc tgttccagat gtggaaggag aagttgtacc
     7321 aataccagaa aaaccactgg tagaaccaat gacgcctcta taccctgcaa ttcctaatta
     7381 ccctacccct caacttccaa aagatgaaga tctggagatt agtggaggtc atggaccgat
     7441 tgtcgatatc gtcgaagata ctggtacagg tgttgagggc ggcgctcaaa atggtgtggt
     7501 ttcaactcag gaaaataaag atccaatcgt tgacatcacc gaagataccc aaccaggtat
     7561 gtcaggctca aatgacgcga cagttgtcga ggaagacaca gcacctaaac gtccagatgt
     7621 ccttgttggt ggtcaaagtg atccaatcga tatcaccgaa gatacccaac caagtgtgtc
     7681 aggctcaaat gacgcgacag ttgtcgagga agacacagta cctaaacgtc cagatatcct
     7741 tgttggcggt caaagtgatc caatcgatat caccgaagat acccaaccag gcatgtcagg
     7801 ttctaatgac gctactgtta tcgaagaaga tacgaaacca aaacgcttct tccactttga
     7861 taacgagcca caagcaccag aaaaacctaa agagcaacca tctctcagct taccacaagc
     7921 tccagtctat aaggcagctc atcacttgcc tgcatctgga gacaaacgtg aagcatcctt
     7981 tacaattgtt gctctaacaa ttattggagc tgcaggtttg ctcagcaaaa aacgtcgcga
     8041 caccgaagaa aactaaccct cgttagtcta gcgaccctaa gcctttggct ttaagaattt
     8101 ctcattatcc ccctcaaaca ccggtacctc actgagatgg cgctttaaaa aggcgctaat
     8161 ctcaaatcag gtatccccta cggttgagga ggattaatgg gtatcacaac aagaaaaaag
     8221 gtataatgat gaaatatgac taaaaaattt attaaatcag cagtgattct aggcttagca
     8281 agcataacca tcagcatgac gcaacccgtt gaagccattt caaatagctt agaatatcaa
     8341 gcacaatacg aacgtgtcaa agaagaacta gacgcattaa aagaaaaggg cccaaaatta
     8401 ggagaacgtt ccctttattt tagattggtg aataaatttc taagttggtt tggccaagtc
     8461 tgggacccac tcgcaatcaa agaaaccgat tacctgttgg acctcatcgg acaagtcaat
     8521 tacttggtga gtaacaccga gtttcaagcc cctgagtaca aagatgctaa aaacaaacta
     8581 gacttagctg tagcaggcta tgacctctac ctagctagag agaaaaaacg tggtgatgct
     8641 cctgattacc aagtgaccca gctaacgcta gaacagctca aaaccatcaa aaagaagata
     8701 cgagaggccc tcgaaacatt tgcacaagag gtcaaaacaa tccagcaaaa aaacccagac
     8761 ctatttcccc atgcttatgt tttaaacgct aagagcaaag atatcacaga taaagcgcaa
     8821 ccctttttag taaatagtta ctacgatgtc ttttataagc agtttttatt cactaataca
     8881 aatgacgggt acaactataa taaagaagca gacaatctga cctcgtatca aaatctgatt
     8941 aaagagctat taggcttaaa agctgagatc gatcagttaa caaaaggcaa gactggcgat
     9001 gaccgtttag agatgttaat gtggcaatca aagttcacct tatctgattc acggaaaaaa
     9061 tcacacctta gcgcctacta tacacaaggc gagtatgtcg ataaggtggt cctatctgaa
     9121 gaagctccca acttgagaac agcaaaagaa aaggtcaaga ccttaaaagg gatcttgcat
     9181 gattactatg ttgaaactaa taagccagaa aaagctaaaa agtatcatgt cgaggacgag
     9241 accttgccgt ctcaacctga ggctcctgct acacctgaag ctcctgctcc tgttcctgct
     9301 cctgttcctg ttcctggtca aaaaccagca gaagatgaga gatcgccaca agctacggag
     9361 ccagctaacc cgtcaaaaga ggattcctca acggacgcat ctcagggatc tcacgatagt
     9421 gaaaacccag cgacagacag tccttctcaa cctcaggcac cagaccaagg caatcaccaa
     9481 agtcaagtgc ctaacgccaa gcctcaaacg gacaagacgg acactcctaa cgccccagcc
     9541 atcccacagg acaccccaaa agcgcctgag gctggtggtc agagtggccc cgctgggaac
     9601 actgaggaga aagctcctga ctctggacct aaagagtctg atcaaagctc atctaaagag
     9661 tccccatccg ttggcgaaga cacgacccct aatcagccag atgtccttgt aggtggtcaa
     9721 agtgagccaa ttgatatcac cgaagacaca atcactgacg ctcctccgac agtatctggg
     9781 cacaacgcat caacacagcc ccaatctgtg gtcgaagaca cagcacctca gcgcccagat
     9841 gtccttgtag gtggtcaaag cgagccaatc gatatcaccc aagataccca accgggcatg
     9901 tcaggctcta atgacgctac cgttatcaat gaagatacaa aaccaaaacg cgtcttccac
     9961 tttgacaaca aagagccaca agcatcagaa aaagcggcag agcaaaaact tgctcctcat
    10021 gatagccaca caacccctca agcttcagat gatacagcag cccctcactt gcctgcatct
    10081 ggagataaac ctgaaaaagt gtttgcactt gctgccttga caatggtcgc agctgcaggg
    10141 ctactcagca aaaaacgtcg caacaccgac aaaaactagt cgctgttagg cacaacgcat
    10201 cactaaggcc agtgcatttg cactggcctt agttgggttt ttatcaaata gagggttttt
    10261 catgacaatt gttagcactt cggtgtaaaa taaaagtaat aaccagcaaa ggagcaacct
    10321 atgacaaagc aagatcagtt aatcgttgaa aaaatggaac aaacctatga ggcctttagc
    10381 ccaaagctag caaatctcat agaagcccta gatgccttta aagagcacta tgaggaatat
    10441 gccacactta gaaattttta cagtagtgac gaatggtttc ggcttgcaaa ccaaccttgg
    10501 gatgatatcc cgtgcggtgt cctatctgaa gacctcctct ttgatatgat cggtgaccat
    10561 aaccaactac tagctgatgt cttagacttg gcccctatca tgtacaagca tatgtaacca
    10621 ttgttaacgt cacagatact acgatgacag gacaaaaaaa gcttgaaaat ctcaaaaaaa
    10681 aacagataaa aaacagataa tcgttagaaa aacagtagat gcgtctgtta aactaaaggt
    10741 atcaaaacaa aaggagcatt tcatcatgaa attcaaaaaa gtattagtca ttcctgcatt
    10801 agcattagca gcaacatgct ttttaacagc ttgtggcaca aaaaaagatt ctaaaaaaga
    10861 agaagttaaa gaaatcaaaa tgtctgacat caaagacgac gcagttagca aaaaaacaaa
    10921 agtggttgat ggcgaagaag ttacagaata caccacaaaa gatggaaacg tgattcagat
    10981 tcctgcagga aacgaagaag gcatggaatc aaaagatgct ggtggatcag gcgctcctgc
    11041 taaaaactaa gggttacaac aaagagcata aggtgactcg caaacgttac gcatgacatg
    11101 aggcagtctg cctaagatgg taactcactt atcataatgc ctaaatagcc aaacacacta
    11161 actcgcaaat gtacaaaatc atatacccgt gcttatgtga gcgaacggtt atatgatttt
    11221 tgttttccaa aaacaagaaa agaacgctaa aatagccagt ttgtcgtgtt tcaagctacc
    11281 aaaaaggaag tgctaagaag tgctaaaatg aaaaaaagat ccattatacc tgtcatctta
    11341 ctgttggtca tcggattaat cgttggtgtt aagcgtcatc atgactagta ggtcttgcta
    11401 gtgaccgttc aaaagggata acgttggtat ggtataacca taaaaatcgt ataaaaagaa
    11461 aaaatagcta ttgaggagaa agtatgtttc agttaagaaa aaaaatgacg cgcaaacaat
    11521 tagccttgtt gagtgctgga gtgttgacct gtgtggttgg tggtagctac ttgataatga
    11581 accatcaaca acaagaaatt gtctctagtg tcaacaaagt aaaagcctta accataaaag
    11641 aagccatgga acaaggaaaa gatatcagct tgaccttagc tggcgaagta acagctaaca
    11701 acagcagcaa agtcaaaatc gactcaagta aaggagaagt caaagatgtc tttgtcaaaa
    11761 aaggcgatgt tgtcaaagta gggcaaccct tgtttagtta tgaaacgtca caacggttaa
    11821 cggctcaaag ttcagaattt gatgttcaaa ccaaagccaa tcaactccaa gttgctaaaa
    11881 ccaatgcagc attgaagtgg gaaacctaca atcgcaaggt caatgaaatc aataccctaa
    11941 aatctcgcta caacactgca ccagatgaga gcttactaga gcaaattcgc agcgcagaag
    12001 acagtgtatc ccaagcacta agcgatgcca aaacagcaga tagcgatgtc aaaaccgctc
    12061 aaatcgaact cgataaagct aatgctactg ccacaacgga aaaaggtaaa ctagagtatg
    12121 acactgttaa gtcagacact gcaggaacca ttgttagcct aaatactgat ttgccaaatc
    12181 aatcaaaatc caaaaaagaa aatgaaactt ttatggaaat tatcgacaaa tcaaaaatgt
    12241 tagtcaaagg taacatcagt gaatttgacc gtgacaagtt aaaaatcggt caaaaagtcg
    12301 aagtgattga ccgcaaagac aactctaaaa aatggactgg aaaagtaacc caagttggca
    12361 acctcaaagc agaggaaaaa ggccaaggtc aaggccaagg tggcaatgac caacaagaca
    12421 atccaaacca agcaaaattc ccttatgtta tcgaacttga ccaatcagac aagcagccac
    12481 tcattggttc acacacctat gttaatgtgc tcaacaatgt tccagaagct ggcaagatcg
    12541 tattgaaaga aacctttaca atggcagaaa atggaaaaac ctatgtgtgg aaagttgata
    12601 aaaacaaggt caaaaaacaa gaaatcaaga ctaagccctt ctcaaaaggt tatgttgagg
    12661 tgacaagtgg cttgactatg caagataaga ttgctcagcc gcttcctggc atgaaagacg
    12721 gtatggaggt aggaagtatt gttaaacctt aaagatattc gaaaaagcta tcatcttggg
    12781 actgaagaat ttgcgatttt aaaaggaatc gatttagaag ttaacgaggg tgacttttta
    12841 gccatcatgg gaccatcagg ttcgggaaag tcaacattga tgaatatcat tgggtgttta
    12901 gataagcctg gctctggctc atatgccatt gaaggcagag acgtgtcatc cttatctgat
    12961 aatgaacttg ctgatttgcg taatcaaaaa atcggctttg tttttcaaaa ctttaacctg
    13021 atgcccaagc taacagcttg tcaaaatgtc gaattgccct tgacttatat gaatgttcct
    13081 aaaaaagagc gtcgcaaacg agcgctagag atgttaaagc tagtaggact agaagaacgc
    13141 agtgaattta aaccgatgga gctatctggt gggcaaaaac agcgtgtagc gattgcaaga
    13201 gctttagtca ctaacccgag ttttatcctt ggtgatgagc caacaggtgc actagacaca
    13261 aaaaccagcg tccaaatcat ggacctattt aaacaattca atgataacgg caaaacgatt
    13321 attatcatca cacacgagcc tgaagtagct gccttatgca aaaagacggt gatcctaaga
    13381 gatggtaata tagaacattc cgatatagag taaaggggga aaataatgga agatattatt
    13441 gcagctttaa gctctatatt atcacacaag atgcggtcaa ttttgacaat gctagggatt
    13501 attatcggta ttggtgccat tatcgcaatc ttttcaatca tcgaaggtaa taccgaaaat
    13561 accaaaagac agttgattgg tggcagcaat aacactatta acattgtgtt taacaaaaaa
    13621 agcagtattg accctaaatt tccagacaag tcaaatgcta aaaaaccaga ctatcttcct
    13681 tttatggctg aagaagagct tagcaaaata cagcaagtca aaggtgtcaa aaacgccctg
    13741 atctcatatg gtattgatga caaagtctac catctaggcc aaaaatcatc tgcaaagatt
    13801 tctgccatca caaaaaacgt agctgaagtg aggcgcatga catttattaa aggcagtgac
    13861 ttttcagaca aagactttat tgaccaaaaa caggtgattt accttgaaaa aagcttgtat
    13921 gagtcactat ttccaaaaga cgatggcctt ggaaaatttg tcgaagtgat gggaaatccc
    13981 ttcagggtca ttggggtgtt tgaatcaaaa gaacaaagtg gcctcacaag tgggactgag
    14041 aaaattgctt atatcccgct acatcagtgg tataatatta atggtgtagt ggatgcgaca
    14101 cctgagatca cgatccagac ctatcgcgca gatgacctca aacctgtagc aaagcgtgtc
    14161 agtgatatgc tcaatcaaac gatccctaaa tcagattata tgtttggtgt catgaacctt
    14221 aaagaatttg agcgccaact agacaaccta aacaaatcta attttgtgtt attagctggt
    14281 attgctagca tctctcttat tgtaggtggt attggtgtga tgaacattat gctagtatca
    14341 gttaccgaaa gaacgagaga aattgggatc aaaaaagcgc ttggcgctag acgtaagctt
    14401 attttaaaac aatttttaat tgaagcggtc attctaacct tgctaggtgg tgtgataggg
    14461 gttatttcag ggatggtatc aggattgatc atcacaagat cactcgagta tccctatatc
    14521 ctatccttgt tctcagttgt cttgagttta gcgttttgtt gtattatagg gattgtcttt
    14581 ggactattac cagccattaa agcgtctaag ctagacccaa ttgaagcatt gcgatttgaa
    14641 taatcaacca aacaagcata aaagtcaaaa aggatgaccc tttgatgtca tccttagctg
    14701 cttgatacac taaacgccaa tctagaaagg gaagttatgt ttaaaatatt agtagttgaa
    14761 gatgatgata ccatcagtca agtgatttgt gagtttttaa aagcgaataa ctatgatcca
    14821 gattgtgtat ttgatggcca agctgcctta gacaagtggc agacaacgtc ttatgatctg
    14881 attattttag acatcatgct cccttcctta agtggcttag aagtgctaaa aacaattcga
    14941 aagacatcag atgtccctat tattatgcta acggcattag atgacgaata cacacagctt
    15001 gtaagcttca accatttgat tagtgattat gtgaccaaac cattttcacc actcatttta
    15061 atcaaacgta tcgaaaatgt attacgcgtc tctactccag atgaaaagcg ccaaatcgga
    15121 gacttacttg ttgatgagac tgaacatagc gtctattggc aaggaacatt ggtcaaacta
    15181 accaaaaaag aatatgacat tattgattat ttagcaaaac gtcaccaaaa aattgtcacc
    15241 agagaccaat tgatggatga catatggggc tattctgaac tggatacgcg tgtccttgac
    15301 aaccacatta agaaccttcg taaaaaaatg acgggtattc ccctcaagac aattacaggc
    15361 atgggctatt tattgggaga gcgtgagtga gactaatcaa aaaaaccttt ttggtcatca
    15421 atggcttaat tattgtagtg gttacctcta ttttgttagt gctttatttt gcaatgccaa
    15481 tttattacac caaagtcaaa gacaaagaag ttaagcgtga atttgaccaa acaagcaagc
    15541 aaatcaaagg caaaacggta accgagatta gagatatcct aacaaaaaaa atcaacaaag
    15601 ataatatttg gtacagctta gtagacagtg ataaccaact gctctatcct tccttgcagc
    15661 tgttagacgg cgtcagtgaa agcaaagaca gtcaaaatgt taatatcgtc accacctttg
    15721 acaactctta ttctaatgtt aaggtgatga gtcaaaaagt aaccctacgc gatggaaaaa
    15781 aaatgacatt gcttgggcaa tcttcacttc aacccgtaac agatgctagc aaagttctcc
    15841 ttgacctcta cccgtcacta ttgatgtttt cagtgaccgt agggagtatt gtcgcctact
    15901 tatatagtag gacttctagc cggcgtatcc ttagcatgtc acaaaccgcc aaaaaaatgg
    15961 tcaacctaga accaaactta acgtgtacca ttcacggcaa agatgaaata gcaatgttgg
    16021 ctagtgatat taaccgtttg tatgcgagtc tttcaacgag tatcaaatcc ttgcaaaaag
    16081 agtatgaaaa agcttctgat tcagaaagag agaaatctga gtttttacgc atgacatcgc
    16141 acgagctaaa gacaccaatc accagtgtta tcggaatgat tgatggaatg ctctataatg
    16201 ttggtgactt tgcagatcgt gacaaatatt tgcgaaaatg ccgagacgtc cttgaagggc
    16261 aagcccaact ggttcaatcc attttatctt tatctaagat tgaaacccta gcttctcaaa
    16321 atcaagaact gttttcacta aaaagcagcc tagaagaaga aatggaagtc tttctcgtct
    16381 tatcagaact aaaacatctc aaagtgacta tcaatctcga agaacaattc gtcaaagcca
    16441 ataaagtata cctactaaag gcgattaaaa atattattga caatgccttt cactatacca
    16501 aatcaggcgg ccaagtgatg attcaactaa aagacaacca actagtgatt aaaaatgaag
    16561 cagagacatt attgacacaa cagcagatga aacagttatt ccaaccgttt tatcgaccag
    16621 attatagtcg taacaggaaa gacggtggta caggattggg gctgtttatc acccaccaga
    16681 ttcttgatca gcatcatctg gcttatcgct ttgttgttct tgatcaaaga tggatggtat
    16741 ttacgattga ttttccatcc catcatgacg attgatccac actagtagga cgacaaaagt
    16801 catcaatcac taaccaagcc ttgaccttta cccatcattg tttaggcaag tataaaaaag
    16861 aggagagaaa tcatatatga agaaaaggaa attgttagca gtaacactat taagtaccat
    16921 actcttaaac agtgcagtgc cattagttgt tgctgacacc tccttgcgta ataacacatc
    16981 atccactgat cagcctacta cagcagatac tgatacggat gacgagagtg aaacagcaaa
    17041 aaaagacaaa aaaagcaagg aaacagcgtc gcagcacgac acccaaaaag accataagtc
    17101 atcacacact cacctaaccc ccccttcaaa tgatactaag cagaccgatc aggcatcatc
    17161 tgaagctact gacaaaccaa ataaagacaa aaacgacacc aagcaaccag acagcagtga
    17221 tcaatccacc ccatctccca aagaccagtc gtctcaaaaa gagtcacaaa acaaagatgg
    17281 ccgacctacc ccatcacctg atcagcaaaa agatcagaca cctgataaaa caccagaaaa
    17341 atcagctgat aaaacccctg aaaaaggacc agaaaaagca actgataaaa ccccagaacc
    17401 aaatcgtgac gctccaaaac ccatccaacc tcctttagca gcagctcctg tctttgcacc
    17461 gtggagagaa agtgacaaag acctgagcaa gctaaaacca agcagtcgct catcagcggc
    17521 ttacgtgaga cactggacag gtgactctgc ctacactcac aacctgttgt cacgccgtta
    17581 tgggattact gctgaacagc tagatggttt tttgaacagt ctaggtattc actatgataa
    17641 agaacgctta aacgggaagc gtttattaga atgggaaaaa ctaacaggac tagacgttcg
    17701 agctatcgta gctattgcaa tggcagaaag ctcactaggt actcagggag ttgctaaaga
    17761 aaaaggagcc aatatgtttg gttatggcgc ctttgacttc aacccaaaca atgccaaaaa
    17821 atacagcgat gaggttgcta ttcgtcatat ggtagaagac accatcattg ccaacaaaaa
    17881 ccaaaccttt gaaagacaag acctcaaagc aaaaaaatgg tcactaggcc aattggacac
    17941 cttgattgat ggtggggttt actttacaga tacaagtggc agtgggcaaa gacgagcaga
    18001 tatcatgatc aaactcgacc aatggataga tgatcatgga agcacacctg agattccaga
    18061 acatctcaag ataacttccg ggacacaatt tagcgaagtg cctgtaggtt ataaaagaag
    18121 tcagccacaa aacgttttga cctacaagtc agagacctac agctttggcc aatgcacttg
    18181 gtacgcctat aatcgtgtca aagagttagg ttatcaagtc gacaggtaca tgggtaacgg
    18241 tggcgactgg cagcgcaaac caggctttgt gaccacccat aaacctaaag tgggctatgt
    18301 cgtctcattt gcaccaggcc aagcaggagc agatgcaacc tatggtcacg ttgctgttgt
    18361 agagcaaatc aaagaagatg gttctatctt aatttcagag tcaaatgtta tgggactagg
    18421 caccatttcc tatcggacgt tcacagctga gcaggctcgt ttgttgacct atgtcgtagg
    18481 ggacaaactc ccaagaccat aaccccacaa ccaagaagct acctgcctgt tgaccaatct
    18541 caaaaagaga cagtgtcaac ttagaatgag gttcatcaga tgataaagaa agtgaccacc
    18601 ccgagtcaaa aaataaaaaa acgtgttcgt aatggctatc tcctaaaact aggtacagcc
    18661 tgtttactct taagtatact cagttatggc attggtcttt tgggccagcc tagcatggaa
    18721 aatacgttta tgggaatagc gagtgttgcc atgttaggaa gtgtctgttt ctttattatc
    18781 tttgctctaa accgtatctt tgacgcacta gaggacaatt tgcgagatta gagtaatagg
    18841 tcaaataatc ttccaataat ttagagcaca agctaagtta ttggaagttt ttttgtcttt
    18901 tgagagccat gatgccatca aaaagtcaaa cactccttat gatgtgagag tcacatgact
    18961 aaaattaaaa gcaaaataga ccccaaagcc ctgttttctc aaaacagcca acgaaaaaag
    19021 acaagttaac cagttcataa aaaacatcgt agaaacgttt tcaagagcta atgttggagt
    19081 aaattgactg aagtatgata gaatttttaa tgaatgtgac aataatgtca cagataaaaa
    19141 ccgaaaacag caaaaataat taaattgaat ataaaaaagt taattaagtg agtgataatc
    19201 aagaaatgaa gttcaaaaaa tataaataaa ataaaacaca agaactgcta aaagcagtga
    19261 atgacataaa tgtcgctaaa aaaatagata gaagagtcta aaatttcagt tttaggtcac
    19321 gttttatgaa tggaaatcag aatgatgacc ttaagttgtg atttgcgcgt cattgaccgc
    19381 tttcttcgtt ttaatgaaag aatttgaaag atattttatg gtatactttt ccttaatatg
    19441 gttcatacgg acttgataaa tctatttaca aaaataataa aaaattaaga taaggacatg
    19501 aagttaatgc atgtaagtaa attgtttact agccaacaat ggagagaatt gaaactgatc
    19561 tcatatttaa cagaaaactc caatgctatc ggagttaaag ataaggagct tagcaaggct
    19621 ctcaatattt ccatgttaac attacagtca tgcctcacta atatgcagtt tatgaaagaa
    19681 gtaggtggaa ttacctacaa agacggctat attaacattt ggtatcacca gtgttgtggg
    19741 ctacaagagg tctatcaaaa agctcttcga gaatcgcctt ctttgaaatt actggagctt
    19801 ttgtttttca gagactttag ttccttagaa gagttagctg aagagctgtt tgtcagctta
    19861 tctaccctca aacgcctcat caaaaaaacc aacacttact tgtctcatac ctttgctatt
    19921 agtatcgtga caagtccagt gcaggtgtca ggagatgaga ggcaaattcg cctgttttac
    19981 ctcaagtatt tttcagaagc ctataaaatc tcagaatggc catttgggga cattttaaac
    20041 ctgaaaaact gtgaacgcct attgagctta ttgatcaaag aagtagatgt caaagttcat
    20101 tttacgctat ttcagcactt aaaaatctta agtggcgtca acttgatccg ttactacaag
    20161 gggtattctt gcagttataa caataaaaaa accagtcacc gtttttctca acttatccaa
    20221 cattcctcag aaatccagga tctttctcgc ttgttctacc tcaaatttgg gttacatctc
    20281 gacgagtata ccatagctga aatgttttct aaccatctta acgacaagct agaaattggc
    20341 tgtgcttttg agattatcaa tcaagaccca atatcaggag gcagacaagt aaccaactgg
    20401 atccatctat tagatgagat ggaaatcaag ctcaatctca gcatcaccaa taaatacgaa
    20461 gttgccgtaa ccctccataa cgcttcggtg ttaaacgaag aagacattac agccaattat
    20521 ttgctgtttg attataaaaa gagttacctg aacttttacc aaaaagaaca ccctcgtatc
    20581 tatgaggcct ttgtgacaag tgtcgagaag ctgatgcagg cagataatgc tcaagttagc
    20641 aaagaattga tcaatcagct cacttattgt ttctttatta cctgggaaaa tagtttctta
    20701 aaagtaaatc aaaaagaaaa agtacgactc ctagtgatag agagaagtta caatagtgtc
    20761 ggtaattttt tgaaaaagta catcggtgag tttttcagca ttactaattt tgatgagtta
    20821 gattgtctga caattgatct agtagagatt gaaaaacagt acgatgttat cgtgacagat
    20881 gttatggtgg gtaaaagcga agagttagag attttctttt tctacaagat gatcccagaa
    20941 gctattattg atagattaaa cgaatttttg aacgttagtt tcacagataa caatgttatg
    21001 gtcaaaaacc tcgaagcccc ttctccttca aaatctcata gcgacaaaga ggtacaaaag
    21061 ccagaaaagc cagacaattc tgttaaacaa gcaacatcag caacatcatc ataggatttc
    21121 agacgtcatg gtaagaaaaa aagaatcatt tacagcccat agaggtaagg tcaaaagctg
    21181 aaaacagctc aaaaaaactg acctttacct tttggctttt tttatttaga ataattttat
    21241 tggagagatg cttaatctaa tttaagcaca attcttagaa agttaagaaa taaggagtaa
    21301 acaatgtcta aaagaaatcc aaacaaacac tactcactga gaaagttaaa aacaggtact
    21361 gcatcagtag cagtagcttt gacagttttg gggactggat tagcaaacac aactgatgta
    21421 aaggctgaga ctgagcatgt tgatgttgtg ctttcagcaa aagaagctaa caaagtattc
    21481 gaagagcgca aagccttgga aaaacaagcg cgtgatttgg gtgacactat taatcacatg
    21541 tcacaaacca ttagtgagca aagccgcaag attgcagcat taaagtctga agcagaactt
    21601 aaaaaccaac aagctcttga agctttaaat aataaaaatg agcagattgc taaattaacc
    21661 aacgaaaacg cacagttaaa agaagccatt gaaggttatg tgcaaactat ccaaaacgct
    21721 agccgtgaaa tcgcagcaaa acaacaagaa cttgcagctg caaaaagcca gttagaggca
    21781 aaaaatgctg agattgaggc attgaaacaa caagatgcct ctaagactga ggaaattgct
    21841 aaattgcaat cagaagcagc aactttagaa aacctcctag gttcagctaa gcgtgaattg
    21901 actgatctgc aagctaagct agatacagct aacactgaaa aagaaaagct ccaatcacaa
    21961 gcagcagccc tagaaaaaca actagaagca actaaaaaag agttagctga tttacaggct
    22021 aaattagcag caaccaacca agaaaaagaa aagttagaag ctgaagcaaa agctcttaaa
    22081 gagcaattag caaaacaagc tgaagagctt gctaagctaa aagcagataa agcttcagga
    22141 gctcaaaaac cagatactaa acctggcaat aaagaggttc caacaagacc gtcacaaaca
    22201 agaacaaaca ctaataaagc tcctatggcg caaacaaaga gacaattacc gtcaacaggc
    22261 gaagaaacaa ccaacccatt cttcactgca gcagctgcaa cagtgatcgc atctgcaggc
    22321 gtacttgccc taaaacgcaa agaagaaaac taagcccaat ccacactatc ttttctagcc
    22381 caagaaaaaa acaaaaaaag aggaagacca ttcctctttt tttgaacggt taaacagcaa
    22441 aaaggtcaaa aaggtactaa agtctcaaaa acctggtctt taccttttac cgcttattct
    22501 ttagaataga attattagag agaagtctta gaaaaatgag gctaattccc taaagatgaa
    22561 aaaataagga gcaaataatg gctagaaaag atacgaataa acagtattcg cttagaaaat
    22621 taaaaacagg tacagcatcc gtagcggtcg ctgtggctgt tttaggagca ggctttgcaa
    22681 accaaacaga agttaaggct gcggagtctc caaaaagtac tgagacttct gctaatggag
    22741 ctgataaatt agctgatgca tacaacacat tgcttactga acatgagaaa ctcagagatg
    22801 agtattatac attaattgat gctaaagaag aagaacctag gtataaagca ttgagaggcg
    22861 aaaatcaaga tcttcgggaa aaagaaggaa aataccagga taaaataaaa aaattagaag
    22921 aaaaagagaa aaatttagaa aaaaaatcag aagatgtaga acgtcactat cttaaaaaac
    22981 tagatcaaga acataaagaa caagaagaac gtcaaaaaaa tctagaagaa ctcgaacgtc
    23041 aaagtcaacg agaaatagac aagcgttatc aagaacaact ccaaaaacaa caacaattag
    23101 aaacagaaaa gcaaatctca gaagctagtc gtaagagctt aagccgtgac cttgaagcgt
    23161 ctcgtgcagc taaaaaagac cttgaagctg agcaccaaaa actcaaagag gaaaaacaaa
    23221 tctcagacgc aagccgtcaa ggcctaagcc gtgaccttga agcgtctcgc gaagctaaga
    23281 aaaaagtaga agcagactta gcagaagcaa atagcaaact tcaagccctt gaaaaactaa
    23341 acaaagagct tgaagaaggt aagaaattat cagaaaaaga aaaagctgag ttacaagcaa
    23401 gactagaagc tgaagcaaaa gctcttaaag agcaattggc taaacaagct gaagaacttg
    23461 ctaaactaaa aggcaaccaa acaccaaacg ctaaagtagc cccacaagct aaccgttcta
    23521 gatcagcaat gacgcaacaa aagagaacgt taccgtcaac aggcgaagca gctaacccat
    23581 tctttacagc agcagctgca acagtaatgg tatctgcagg tatgcttgct ctaaaacgca
    23641 aagaagaaaa ctaagctatt agactgatgc taaagttaag agagaatcaa atgattctct
    23701 ctttttgagt ggctaagtaa ctaacaatct caattagacc aaaaaatggg aatggttcaa
    23761 aaagctggcc tttactcctt ttgattaacc atatataagt aaaaacatta ggaaaataat
    23821 agtaatatta agtttgtttc ctcaataaaa tcaaggagta gataatggct agacaacaaa
    23881 ccaagaaaaa ttattcacta cggaaactaa aaaccggtac ggcttgagta gccgttgctt
    23941 tgaccgtttt gggtgcaggt tttgcaaacc aaacggaagt aagagctgaa ggggtaaacc
    24001 cgactacgaa cttgccagag aaggctaaat atgccgcagt gaaagatgag aatactggtt
    24061 tacgtggtga tcagaaaaaa ttagtaaaaa aacttgaaga agaacaagag aagagcaaaa
    24121 atctagaaaa gcaaaaacag gagttagaaa accaagccct taactttcaa gacgtaattg
    24181 aaactcagga aaaagaagat ctcaaaacaa ctttagctaa gactactaaa gaaaatgaga
    24241 tttcagaagc tagccgtaaa gggttaagcc gagacttaga agcttctcgt gcagctaaaa
    24301 aagagctaga agctaagcac caaaaattag cagaagaaca tcaagtttca gagactagcc
    24361 gtaagggtct aagccgtgac cttgaagcat cacgtgaagc taataagaag gttacatctg
    24421 agttaacaca agcaaaagct caactctcag cgcttgaaga aagtaagaaa ttatcagaaa
    24481 aagaaaaagc tgagttacaa gcaaaactag atgcacaagg aaaagccctc aaagaacaat
    24541 tagcaaaaca aactgaagag cttgcaaaac taagagctga aaaagcggca ggttcaaaaa
    24601 cacctgctac caaaccagct aataaagaaa gatcaggtag agctgctcaa acagctacaa
    24661 gacctagcca aaataaagga atgagatcac aattaccgtc aacaggcgaa gcagctaacc
    24721 cattctttac agcagcagct gcaacagtga tggtatctgc aggtatgctt gctctaaaac
    24781 gcaaagaaga aaactaagcc tttagaactt ggtttttgta acggtgcaat agacaaaagt
    24841 aagcaaggcc aaaaactgag aaagtcctaa aaagctggcc tttacccctc aaaattaatg
    24901 ttttataata aagatgttag taatataatt gataaatgag atacatttaa tcattatggc
    24961 aaaagcaaga aaaatagctg tatcatatgc aaataacccc tgtttgctct ttaaaaaaga
    25021 cgttatcctt atttctccac gcacagatgg acagctagga gagaatcgtt tgattctctc
    25081 ttttcttaat ggtcataaag acaaagtctc tcatcatgaa aggacgacac attgcgtaaa
    25141 aaacaaaaat taccatttga taaacttgcc attgcgctca tgtctacgag catcttgctc
    25201 aatgcacaat cagacattaa agcaaatact gtgacagaag acactcctgc taccgaacaa
    25261 gccgtagaac ccccacaacc aacagcggtt tctgaggaag caccatcatc atcaaaggaa
    25321 actaaccctc cacaaattcc tgatgacgca gaagaaacag tagcagataa ggctaatgat
    25381 ccagcccctc aagctcctgc taaaactgct gatacaccag caacctcaaa agcgactatt
    25441 agggatttga acgacccttc tcaggtcaaa accctgcagg aaaaagcagg caagggagct
    25501 gggactgttg ttgcagtgat tgatgctggt tttgataaaa atcatgaagc gtggcgttta
    25561 acagacaaaa ccaaagcacg ttaccaatca aaagaagatc ttgaaaaagc taaaaaagag
    25621 cacggtatta cctacggcga gtgggtcaat gataaggttg cttattacca cgactatagc
    25681 aaagatggta aaaccgctgt cgatcaagag cacggcacac acgtgtcagg gatcttgtca
    25741 gggaatgctc catctgaaac gaaagaacct taccgcctag aaggtgcgat gcctgaggct
    25801 caattgcttt tgatgcgtgt cgaaattgta aatggactag cagactatgc tcgtaactac
    25861 gctcaagcta tcagagatgc tgtcaacttg ggagctaagg tgattaatat gagctttggt
    25921 aatgctgcac tagcttacgc caaccttcca gacgaaacca aaaaagcttt tgattatgcc
    25981 aaatcaaaag gtgttagcat tgtgacctca gctggtaatg atagtagttt tgggggcaaa
    26041 acccgtctac ctctagcaga tcatcctgat tatggggtgg ttgggacacc tgcagcggca
    26101 gattcaacat tgacagttgc ttcttacagc ccagataaac agctcactga aactgctacg
    26161 gtcaaaacag acgatcatca agataaagaa atgcctgttc tttcaacaaa ccgttttgag
    26221 ccaaacaagg cttacgacta tgcttatgct aatcgtggta cgaaagagga tgattttaag
    26281 gatgtcgaag gtaagattgc ccttattgaa cgtggcgata ttgatttcaa agataagatt
    26341 gcaaacgcta aaaaagctgg tgctgtaggg gtcttgatct atgacaatca ggacaagggc
    26401 ttcccgattg aattgccaaa tgttgatcag atgcctgcgg cctttatcag tcgaaaagac
    26461 ggtctcttat taaaagacaa tccccaaaaa accatcacct tcaatgcgac acctaaggta
    26521 ttgccaacag caagtggcac caaactaagc cgcttctcaa gctggggtct gacagctgac
    26581 ggcaatatta agccagatat tgcagcaccc ggccaagata ttttgtcgtc agtggctaac
    26641 aacaagtatg ccaaactttc tggaactagt atgtctgcgc cattggtagc gggtatcatg
    26701 ggactattgc aaaagcaata tgagacacag tatcctgata tgacaccatc agagcgtctt
    26761 gatttagcta aaaaagtatt gatgagctca gcaactgcct tatatgatga agatgaaaaa
    26821 gcttattttt ctcctcgcca acaaggagca ggagcagtcg atgctaaaaa agcttcagca
    26881 gcaacgatgt atgtgacaga taaggataat acctcaagca aggttcacct gaacaatgtt
    26941 tctgataaat ttgaagtaac agtaacagtt cacaacaaat ctgataaacc tcaagagttg
    27001 tattaccaag caactgttca aacagataaa gtagatggaa aacactttgc cttggctcct
    27061 aaagcattgt atgagacatc atggcaaaaa atcacaattc cagccaatag cagcaaacaa
    27121 gtcaccgttc caatcgatgc tagtcgattt agcaaggact tgcttgccca aatgaaaaat
    27181 ggctatttct tagaaggttt tgttcgtttc aaacaagatc ctaaaaaaga agagcttatg
    27241 agcattccat atattggttt ccgaggtgat tttggcaatc tgtcagcctt agaaaaacca
    27301 atctatgata gcaaagacgg tagcagctac tatcatgaag caaatagtga tgccaaagac
    27361 caattagatg gtgatggatt acagttttac gctctgaaaa acaactttac agcacttacc
    27421 acagagtcta acccatggac gattattaaa gctgtcaaag aaggggttga aaacatagag
    27481 gatatcgaat cttcagagat cacagaaacc atttttgcag gtacttttgc aaaacaagac
    27541 gatgatagcc actactatat ccaccgtcac gctaatggca agccatatgc tgcgatctct
    27601 ccaaatgggg acggtaacag agattatgtc caattccaag gtactttctt gcgtaatgct
    27661 aaaaaccttg tggctgaagt cttggacaaa gaaggaaatg ttgtttggac aagtgaggta
    27721 accgagcaag ttgttaaaaa ctacaacaat gacttggcaa gcacacttgg ttcaacccgt
    27781 tttgaaaaaa cgcgttggga cggtaaagat aaagacggca aagttgttgc taacggaacc
    27841 tacacctatc gtgttcgcta cactccgatt agctcaggtg caaaagaaca acacactgat
    27901 tttgatgtga ttgtagacaa tacgacacct gaagtcgcaa catcggcaac attctcaaca
    27961 gaagatcgtc gtttgacact tgcatctaaa ccaaaaacca gccaaccgat ttaccgtgag
    28021 cgtattgctt acacttatat ggatgaggat ctgccaacaa cagagtatat ttctccaaat
    28081 gaagatggta cctttactct tcctgaagag gctgaaacaa tggaaggcgg tactgttcca
    28141 ttgaaaatgt cagactttac ttatgttgtt gaagatatgg ctggtaacat cacttataca
    28201 ccagtgacta agctattgga gggtcactct aataagccag aacaagatgg ttcagatcaa
    28261 gcaccagaca aaaaaccaga aactaaacca gaacaagacg gttcaggtca agcaccagac
    28321 aaaaaaccag aagctaaacc agaacaagac ggttcaggtc aaacaccaaa taaaaaacca
    28381 gaaactaaac cagaaaaaga tagttcaggt caaacaccag gtaaaactcc tcaaaaaggt
    28441 caaccttctc gtgctctaga gaaacgatct tctaagcgtg ctttagctac aaaagcatca
    28501 acaagagatc agttaccaac gactaatgac aaggatacaa atcgtttaca tctccttaag
    28561 ttagttatga ccactttctt cttgggatta gtagctcata tctttaaaac aaaacgcact
    28621 gaagattagt acaactgatc agatggctac tattgatgat gagttagatc agtcttcttt
    28681 tcaaaagaag gcatagcagt ataaaaatag atatgtgagg acaatatgcg tagagcagaa
    28741 aataacaaac acagccgcta ttccattcgc aaactgagcg ttggggtaac gagtatagca
    28801 attgcgagtc tctttttggg aaaagttgcc tatgccgtag atagcatgca gccaatctct
    28861 tcgactcaag gaaacagagc caatacatcc aaaaaatggt atgatattaa tgagtatggt
    28921 cttattcctg tagatataag ctttgaagct gccaaaaaag attttaaaga agaagtagaa
    28981 aaatcacttt tgtctgaaga acaacagaaa acgtataatc aaaaaattga agctgcaaga
    29041 aacaaagacg acctattgtt aacgtatcat agtgagtact ctaaagatat taagaaacac
    29101 tcaacgtcta ctgagccagt tgaggcatct gtgcaggaga cacaggcatc agtttcagat
    29161 tcgatggtga cagatgattc aacatcagtt acggctgatt ctcctgagaa aacaccatct
    29221 acagaaacac cggctccatc aattccagag gtcccagaag ccccagcttc atcacctgag
    29281 agtgaggagt cttcagtagc atcttctgag gaaacctcat ctccagaaac tccagtggcc
    29341 ccagaagccc cagctttatc tgaggctcca gctcaaccag ctgagagtga ggagtcttca
    29401 gtagcagctc cttctgagga aaccccatct ccagaaactc cagcggcccc agaaactcct
    29461 gaagagccag cagctccatc tccatcacct gagagtgagg aaccttcagt agcagcttct
    29521 tctgaggaaa ccccatctcc agaaactcca gcggctccag aaactcctga agaaccagca
    29581 gctccagctc aaccagctga gagtgaagaa tcttcagtag cagctacgac aagcccgtct
    29641 ccatcaactc cagccgaatc agagactcag acgccaccag ctgttactaa agactctgat
    29701 aagccatctt cagcagctga aaaaccagca gcctcttcac ttgtttcaga acaaaccgtt
    29761 caacaaccaa cttcaaagag atcttctgat aaaaaagaag agcaagaaca gtcttactct
    29821 ccaaatcgct cattgtcaag acaggttagg gcccatgagt caggtaagta cttgccttca
    29881 acaggtgaaa aagcacagcc actctttata gctactatga ctttgatgtc tctatttggc
    29941 agtcttttag tcacaaaacg ccaaaaagaa actaaaaaat agtattactt aaactgaata
    30001 gtattactta aactgataag agcttattta atagagagaa ccaatttggt tctctctatt
    30061 tttttagatg tgctgcctca tcaaaaagaa gttaggacaa atagctgtca acaattgtct
    30121 tttgcttatc tcttcagttg ctgtgatttt atcatatatt gataaaagat ggcacgccaa
    30181 acgcatgaag aaactttt
//
LOCUS       DATUYR010000018        29260 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.018, whole genome shotgun sequence.
ACCESSION   DATUYR010000018 DATUYR010000000
VERSION     DATUYR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29260
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.018"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(113..2020)
                     /locus_tag="VCI33_000734"
     CDS             complement(113..2020)
                     /locus_tag="VCI33_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP2831670.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDKTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMMDYDLLAERFRQQGGFTYEAEIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQETLME
                     EWETIAEQIES"
     gene            complement(2066..2392)
                     /locus_tag="VCI33_000735"
     CDS             complement(2066..2392)
                     /locus_tag="VCI33_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831671.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            complement(2422..3207)
                     /locus_tag="VCI33_000736"
     CDS             complement(2422..3207)
                     /locus_tag="VCI33_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEP2831672.1"
                     /translation="MASIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            complement(3340..5001)
                     /locus_tag="VCI33_000737"
     CDS             complement(3340..5001)
                     /locus_tag="VCI33_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP2831673.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            complement(5205..5963)
                     /locus_tag="VCI33_000738"
     CDS             complement(5205..5963)
                     /locus_tag="VCI33_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831674.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(5960..6829)
                     /locus_tag="VCI33_000739"
     CDS             complement(5960..6829)
                     /locus_tag="VCI33_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831675.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERYFKGVRLTR"
     gene            complement(7174..8172)
                     /locus_tag="VCI33_000740"
     CDS             complement(7174..8172)
                     /locus_tag="VCI33_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP2831676.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANRQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKNLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVIDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            8526..10178
                     /gene="fbp54"
                     /locus_tag="VCI33_000741"
     CDS             8526..10178
                     /gene="fbp54"
                     /locus_tag="VCI33_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEP2831677.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSEMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            complement(10237..11484)
                     /locus_tag="VCI33_000742"
     CDS             complement(10237..11484)
                     /locus_tag="VCI33_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEP2831678.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDMEGLTEVAREKLLQAVSISPEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLLYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(11474..12655)
                     /locus_tag="VCI33_000743"
     CDS             complement(11474..12655)
                     /locus_tag="VCI33_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP2831679.1"
                     /translation="MPLIPDKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(12713..13189)
                     /locus_tag="VCI33_000744"
     CDS             complement(12713..13189)
                     /locus_tag="VCI33_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEP2831680.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            complement(13410..14450)
                     /gene="rfbB"
                     /locus_tag="VCI33_000745"
     CDS             complement(13410..14450)
                     /gene="rfbB"
                     /locus_tag="VCI33_000745"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEP2831681.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(14694..15287)
                     /locus_tag="VCI33_000746"
     CDS             complement(14694..15287)
                     /locus_tag="VCI33_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEP2831682.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(15287..16156)
                     /gene="rfbA"
                     /locus_tag="VCI33_000747"
     CDS             complement(15287..16156)
                     /gene="rfbA"
                     /locus_tag="VCI33_000747"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEP2831683.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHELAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(16215..17321)
                     /locus_tag="VCI33_000748"
     CDS             complement(16215..17321)
                     /locus_tag="VCI33_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEP2831684.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGDDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KTSGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLMPLGYQVDVRPQKGQ
                     LLDYDVHHIISDNYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKSCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            complement(17361..18149)
                     /locus_tag="VCI33_000749"
     CDS             complement(17361..18149)
                     /locus_tag="VCI33_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEP2831685.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMERDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(18139..18825)
                     /locus_tag="VCI33_000750"
     CDS             complement(18139..18825)
                     /locus_tag="VCI33_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEP2831686.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIT
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(18897..19553)
                     /gene="nth"
                     /locus_tag="VCI33_000751"
     CDS             complement(18897..19553)
                     /gene="nth"
                     /locus_tag="VCI33_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEP2831687.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            complement(19550..20233)
                     /locus_tag="VCI33_000752"
     CDS             complement(19550..20233)
                     /locus_tag="VCI33_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEP2831688.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(20314..20832)
                     /locus_tag="VCI33_000753"
     CDS             complement(20314..20832)
                     /locus_tag="VCI33_000753"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEP2831689.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(20982..23192)
                     /gene="recJ"
                     /locus_tag="VCI33_000754"
     CDS             complement(20982..23192)
                     /gene="recJ"
                     /locus_tag="VCI33_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEP2831690.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYERGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGTEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(23189..23953)
                     /locus_tag="VCI33_000755"
     CDS             complement(23189..23953)
                     /locus_tag="VCI33_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP2831691.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGTFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(23953..24882)
                     /locus_tag="VCI33_000756"
     CDS             complement(23953..24882)
                     /locus_tag="VCI33_000756"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEP2831692.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFVVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(24897..25532)
                     /locus_tag="VCI33_000757"
     CDS             complement(24897..25532)
                     /locus_tag="VCI33_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEP2831693.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(25525..26763)
                     /gene="hflX"
                     /locus_tag="VCI33_000758"
     CDS             complement(25525..26763)
                     /gene="hflX"
                     /locus_tag="VCI33_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEP2831694.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(26836..27735)
                     /gene="miaA"
                     /locus_tag="VCI33_000759"
     CDS             complement(26836..27735)
                     /gene="miaA"
                     /locus_tag="VCI33_000759"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEP2831695.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLTDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            27863..28039
                     /locus_tag="VCI33_000760"
     CDS             27863..28039
                     /locus_tag="VCI33_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEP2831696.1"
                     /translation="MIMEYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(28157..29065)
                     /locus_tag="VCI33_000761"
     CDS             complement(28157..29065)
                     /locus_tag="VCI33_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEP2831697.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
BASE COUNT         8825 a         6354 c         5154 g         8927 t
ORIGIN      
        1 cgttttaccg accttgacat aaaaaaacac atcattttaa aacctctttc acaccttcaa
       61 acaagtaaca aagcgataaa aataagctta gcaaaactgc taaagcttat ttttatgatt
      121 ctatttgttc agcgatggtt tcccattctt ccataagagt ttcttgttct tgatccaatt
      181 ggtcccattc cttttgccaa gcgattaact gcgccgtatc attggaggca tgcatgtctt
      241 cttgaatagc accaatgcgc tcttcaatgg tttcaagtct tgcttcaatc tcctcatatc
      301 ggcgggtcaa gcgacgacgc tctttttgat tagctttttg tagctgataa tcagtggcag
      361 aggcttcttt tgtttcctca actgtctctc ctgctgctag gcgggctagt tcttctagct
      421 ctgccttttt ctcaaggtaa taatcatagt cacctaagta aagggttgag ccattttcgg
      481 taatttccag aaccttagtt gccaaacgat taatgaaata gcggtcgtgg ctgacaaaaa
      541 gcaaggtacc gtcaaagtca atgagggcat tctctagcac ttctttactg tcaatgtcta
      601 ggtggttagt cggctcgtcc agaacaagaa aattattgtt ttccatagat aacttcgcaa
      661 gaagcagacg ggctttttct ccacctgaca gcatagccac cgattttttg acatcatctc
      721 cagaaaagag aaaagcccct aatcggtttc tgatatcgac ttctggcgta gttgaaaaat
      781 cctgccataa ttcttccaaa actgtgttgc tagacgtcaa atgtgattgg gtttggtcat
      841 agtaacctat ttcaacattg gcaccgtatt tcacttctcc tttgatgaaa ggaatctgtc
      901 caataacaga tttaatcaga gtagatttac caataccatt tggaccaaca atagcaatcg
      961 catctaattt gtgaacgtct aggttaatag gctcagataa aacatgttgg ttataaccaa
     1021 tagcagcttt ttcaaccgtc aacaccacat tgccagacgg cttatctgcc tgaaaagtca
     1081 tgtgagctga ttttctagca ctagtcggtt tgtctaagcg ttctattttt tccaattgct
     1141 tacgtctcgc ttgagcacgt tttgtagttg aagccctgac aatatttttt tgaacaaaat
     1201 cttctaactt ggcaatttct ttttgctgtt tgtcaaactg cttttcttcg gctactaatt
     1261 tttcagcttt taaggtcata aaacgagaat aattgccaac gtagcgatcc aatccatgag
     1321 gagttaaatc caaggtcact gttgccacct tatccaaaaa ataacggtcg tgactcacaa
     1381 tgattagagc tccttgataa ttggccaagt agttctctaa ccaggcaatg gtttcaatat
     1441 ctaagtggtt tgttggctca tcaaggacca atagttctgg cttttctaaa agcattttag
     1501 ccaaggccag acgagtgttt tgaccccctg acagctcagc gatagtcatc tgccacatgc
     1561 tttcatcaaa tttaaagcca tttaaaatgg ctttaatctc agcttcatat gtaaagccac
     1621 cttgctgacg aaaacgttct gctaaaagat cataatccat catcagccgt gtcaagtcct
     1681 gacctgaaac ggttgccata tccatttcca tctgacggag acgcttttca tcttggcgta
     1741 atgcttcgaa aaccttgagc atttcctcat atatcgtttt gtcagattca aaacggctat
     1801 tttgagccag ataagacaag gttaggtctt ttttagtatt aacttctccg ctcgttgggg
     1861 tttcttcccc aaccaatagc ttgagtaagg ttgacttacc agctccattt ggccccacta
     1921 atgcaattct gtctctttca tcaacttgca gtgatatgtt ttgaaaaaga acatctcctg
     1981 agaaagagcg ttctaacttg tttccttgta aaataatcat actttcattc taacaaaaaa
     2041 atgccctttt gaggacattt tcagcctagt ttccaaaaga aatcgaactc tttttaagtc
     2101 gttgattacc ttctttagcg ctaacaagga aagtggaatg actattacca ctcccatgac
     2161 gcgtctttgc agatggcttt tgtgttaaga tatcaaaaat ttgcaccact tgctcagtaa
     2221 ctatcgagat cttattggct aaaacggaat cactgtgatt agaatagtac atcagcccta
     2281 taataataat cgttactaat aagatagttt gtaaaaaccc tttgataaga tgccaaaaca
     2341 ttttcactat tagcttaggc agccacaata tgatcgccag taattgtttc atttgtcacc
     2401 tcatgttgta ctaggaaatg gttaagacaa cctttcttct ttttggtagt ttatcggtaa
     2461 ccactctaac aggacttcta attgctggtt ccaatagtac cactcatgtt taccatgacc
     2521 tttatgataa tcaatgtcaa gaccttgagc ctgaaaatca gcaatagctt tttcattagt
     2581 ggcaaaaaga aaatcctcat agccacacca agcataaaat ttggtttttc catcactttc
     2641 tgccaccata tttttaaggt aatgcttgtc aaggtcaggg tcatcaaact ggccaaaaac
     2701 gccctgccaa taggctaatt ctcccaggtt tccctcaagc aaggtctcag gagaaaaatc
     2761 caaagcgcct gaaaacgatg ccgcataaga aaaccgattg cttttgagtg cccacttaaa
     2821 ggcaccatag ccacccatag agagcccagc aacaaaggtt ttttctcgtt tctgggtcat
     2881 attaggaaag aaagctgcta agacctgcgg taactcttgt gacaacgccc gataatagtt
     2941 aagtccataa gccgtgtctg tataccaacc taggtctgtc gaaggcataa cgactatcaa
     3001 attggtatgc cgcaacaagc gttcaatagc tgttcgtttt tgccaagagt tttcattgcc
     3061 tcccatacca tgtaacaaat ataaaacagg aatatcctta tccccttgat cttttttagg
     3121 gatttcagac tggtcaggat aaatcacatt taccttacgc tccattccta aaactaccga
     3181 atggtattca attgcaatac ttgccattca ttacactcct ttataccaaa ataatccaat
     3241 cacgtaaatc tactgtattt ttgggtgaac agtttcctgg tttaccagtt gttaaaatca
     3301 aagaaggtag gcagtttttg cctaaccttc ctattttcac taacgaactt ccatcacaac
     3361 cggcagaatc gctgggcgcc ttttggtttg ttcaaataag aatttagaca aatcatctct
     3421 gacattccct tttaattcgc cccagtcaaa ggtatctttt tttaagtaat tgccaacagt
     3481 tgtattaact aattctgcac tttctcttaa aatatcatga cttttcttga catacacaaa
     3541 tccacgcgta ttaactttag ccttagaaat aatgcgtttt tcttttttag aaacagtaat
     3601 agcaacaatg aaaataccat cttctgacaa gactttacgg tctcgtaaaa cgatattacc
     3661 cacatcaccg atagcattac cgtcaatcat gacatcactg gcaggtacac ctccttcatg
     3721 gagaaaacct tcgtcattaa gcaccatgat atcgccacgc ttcagaatat gaatattctc
     3781 tggaaaaata ccaacttctt cagctagctt tgcatgggca gctaaatcac gatattctcc
     3841 ttgcactgga aaaagatact ggggttttaa caaattcatc aatagttgca aatcacgccc
     3901 attagcatgg cctgacacgc gtaagttttg agtgattaat ttaacagaac caccggcctt
     3961 gtaaatcaag ttttctacac gcgccaccat agcttcttta gccgtacttg gtgtagttac
     4021 aatataaaca aggtctcctt ctttgatttg aacataacgg tgacgtcccg ctgccatttt
     4081 ttgtaagcta ttaattggct cacccatacg accagcttca aggatgatta attcatggtc
     4141 ttcgaattta gacatatcct taggtttaat taaaagacgc tcatccgtaa tcattaattt
     4201 ttctagacgt aaagctgttc tgacaatatt ttctgcatct gttccggtta aaacaacacg
     4261 acgcccatgg gcagtggcgg aatcaaatac ttgctgaatc cgaacaagat ttgaggctac
     4321 tgcagctaca atgacacggc catcagcatc agagataaca ctgtccattt cttcaccaac
     4381 ttcagattcg ctggcaatct gatcgttgct tgtcgcattg actgaatctg ataaaagagc
     4441 tagaacccct tctttcccaa tctcagctaa acgtagcaaa tccgtctgat aaccttctct
     4501 tgctgcttga tcaaatttga agtcaccggt ataaacaatg tttcctttat ctgttccaat
     4561 aacaataccc atactttctg ggattgaatg cgttgttcta aagaacgaaa ctaggccatc
     4621 tttaaattca atttcagtat cactgtctac cacatgaaaa ttattaaatt tcttagtgct
     4681 gttattactt ttcacaaata gcttggctaa ttcaatagtc aattcagatc caaatacagg
     4741 agctgatact tcagctaaca aataaggaag ggctccaatg gcgtcagcat ggccatgact
     4801 caagaaaatc ccttgtactt tacctttgtt ttcaattaca taatctaaat tggggatgac
     4861 taaatctacc ccaagttgct cattttcagg gtacttgagc cctgcgtcta aaataaacat
     4921 agaatcgtta atttctacca aatagaagtt ttttccgtat tcacggaccc ctcccaaggc
     4981 aatcatttta atatcagtca tgatactcct ttaaatattt tattattaca aacggtcctt
     5041 acaacgtaag tcgaattact gttatgtagc tatgctacta tcttctatag tatatcatat
     5101 ttgaggcgtt ttttctctaa atagccttga tactttcaga ttattttcag tatggaaatc
     5161 aaaaaatcac tgacttatta ttagtcaatg atttagatcg ttgattagtc aaatagttgg
     5221 taatagtcag taatcgtcag ttgttctttt tccatttgat ttaaatcttt aataatgttt
     5281 ccatctttca ttacaatcag acgattccca taagttaatg catcttccat atggtgggta
     5341 atcatcaaag ctgttaagcc atcttttgtc acaaactcat ctgtcaattg cattaaagat
     5401 tgactcgtct tagggtcaag ggcagccgta tgttcatcta ataataagag agctggcttt
     5461 ttcaacgttg ccattaataa acttaaagcc tgtctttgtc caccagacaa aagacctgct
     5521 ggtgtttcta aatgcttttc taggccattt cctgttcgtt taaccaaatc ctcaaaacta
     5581 gctaagtgct ctgttatttt tcgagaagcc aaggagcgtt tttctcctct ttgacgtgcg
     5641 attaagagat tttcagccac tgtcatccga ggggctgttc ccattttggg atcttgaaag
     5701 actcgcgata ggtaaagagc tcttttttct gccggccaat gcgtcacatc ctgaccaaga
     5761 atcctaattt gcccccgtgt caaacttagc gttcctgcaa ttacattaaa aagggttgat
     5821 tttccagctc catttcctcc taaaatagtt aaaaaatcat gttcatagat agttagtgtc
     5881 acattgtcca gtattgtttt agcatcttca aaaccattat ctacgtcaac tgttgcattg
     5941 atcaattcaa taatttttgt catcgtgtta acctcactcc cttaaagtag cgctctttta
     6001 ccactggtat catgaggcac agtgctaaaa caagagcact aattaatttc aaataacttg
     6061 tattaaaacc tagcgtaata actactgaaa ttaaaaattg ataaagaatc gagcctatta
     6121 caatagcaat caaacgttct aaaagagtca gtccagttga gtatagtact tcaccaacaa
     6181 ttatgctcgc caaaccaata acaataactc caatcccttt tgagacatct gcatagccat
     6241 cttgttggct cactagagca cctgataaag ctattaaact attagaaacc acaagtccca
     6301 taacttccat ccaatccgtg ttaataccaa aactcttagc catatcttta ttgtctcccg
     6361 tagcgatata agcttgacct agattagtgt aaaggaaata gatcaaaacg ctaataacaa
     6421 tcacaacagt tatcagacct gttaaaaggc tatttaaatc aattgaaaaa ggtaaacaat
     6481 cctgaatacg tttatgatca tgtaaaccca agtttgctct tcccattacc attagcataa
     6541 tggagttaca tgacgtcatc accaaaattc cagctaaaag tgttggcatt tttcctttcg
     6601 tatataacag ccctgttaaa aaacctgcta aagcacctga taacatacca agtaaggttg
     6661 atagaaatgg attccaccct aagctaatag cggtgaccgc aacagctcct cctaaaggaa
     6721 aagaaccttc tgtagtcatg tcagggaagt ttaaaatcct aaaggtcagg tagattccta
     6781 atcctaaaac tccccatata aggccttgtg aaactgatga aataatcata tttttcctca
     6841 tatttatatt agcgggtaac tctcgccagt ataaaccctt tctttacatt aatttttaga
     6901 tttgttttat acttagttca gcaccgtggc ttagtctttt ttccaatcac tttaacgaaa
     6961 ctaagacaga aaacaaaaaa atcgagaatc atgtttaaac ttactcaaaa ggcaagattt
     7021 tttacgtgtc tgaaatagag cattttaaaa ctaaaaatga taaaaataac ccttacatcc
     7081 tcaagacgat tgtctgactt ggcatccaaa ttcatctcag tgagggtgtt ttttatggtt
     7141 ttttcttgac cttttatatc acatgtcatt gtattaattc tcaataaaag aagctgaagg
     7201 aaaatcttcc ctgttaatag caatacctaa ttctgctgct acattcttat taattagcgg
     7261 ttgaccaata tccacaactt tgacaggaac atctataaca cgctttcctt tgattaattt
     7321 taacacctga ttagctgttt gaactcccaa atcgtactga ttttgagaaa tggctgccaa
     7381 accacctttt tcaaccattc gatcaacgct agtgtagaca ggaatctttg cagccttact
     7441 ggttgtcatg actgatgaaa atgcacttgc tatagtattg tcttggggga taaataccgc
     7501 gtctacttta cctaacataa ctgacatcgt ggcaggaact tcgttagtag aaggaaccgc
     7561 gtatgaaata acttgatagc cctttttgcg agctaaccgc ctaaactctt taacttgaga
     7621 taaggaatta tcctcattac ttgcatataa aattccaact gttttgacat gtggtgtcag
     7681 atttttcatt aaatcaattg tctgtttaac aggcacttta ttagatagac cagttacatt
     7741 agcttcaggt tgatccaatt gcatcactaa tctagatcct actggatcag atactgctga
     7801 catgacaact gggatatcct tgctggcagc tgctaagcct tgcgccgccg gggttgcgat
     7861 accaatcacg atatcacttc ctgattgcac taattgccta ctcattgttt ggattttact
     7921 ctgatccccc tcagcattca taaccttaat cactacattt tgtttttgag gcatctgctt
     7981 tttgagttga tcttcaatac ctcgcttaat atcatcaaga gcctcatgag taacgaattg
     8041 taagatacca attgtaatgt tttgtcggtt agccaaatca cggttttcct ttgatactcc
     8101 ttttgataat agagagccta tcactattac agttaaaaca aggagggtag ctattaaact
     8161 cttgtttttc atctgaattc ctcacatttc atgtcaatct ctttaaaacg ttgatgatga
     8221 gaatacttct caaaagaaaa agcacttagt aatgattact aggcgccctt gtgaaatcca
     8281 atgtcagcgc atagatatct ttttagaagt actctaaaag tcgtatctat gccaattcat
     8341 aaacacaact tatctatgcc agccttgcca atttactaag tgttgtgttt ttaccatcat
     8401 tgacttcctt tctcgttttt ttaacattat aacctaaatc aaaaaaattg caagctcttt
     8461 cgtccatatt tttagtcaag tatttgtggt ataatgaaga gattagaatt cagaaaggag
     8521 aaaccatgtc ttttgacggc tttttcttac accatttaac caacgaatta aaagagaact
     8581 tgctttacgg tcgtatccaa aaggtcaatc aaccctttga acgggaactt gtcttaacca
     8641 ttcgtaacca tcgcaagaat tataaattgc tattgtctgc ccatcctgtt tttggaaggg
     8701 tacaaatcac ccaagcagac tttcaaaatc ctcaggtacc taataccttt accatgatta
     8761 tgcgtaaata cctccagggt gcagtcattg agcagttaga acagattgat aatgaccgca
     8821 ttatcgaaat caaggtatct aataaaaatg aaatcggaga tgctattcag gcaaccctaa
     8881 tcattgaaat catggggaag cacagcaata tcatcttggt ggaccgtgca gaaaataaaa
     8941 tcatcgaatc cattaaacat gttggctttt cacaaaactc ttaccgcacc attttacctg
     9001 gctctaccta cattgagccc cctaaaacag ctgctgttaa tccttttaca attacagatg
     9061 ttcctctatt tgaaatcctc caaacacaag aactaacagt taaaagcttg caacagcatt
     9121 ttcaaggttt ggggcgagac accgcaaaag agttggccga gcttctgacc acagacaaat
     9181 taaaacggtt ccgtgagttt tttgctagac ctactcaggc aaatctgacc acagcttctt
     9241 ttgcgcctgt tcttttctca gatagccatg cgacgtttga gactttatca gagatgcttg
     9301 atcactttta tcaagataag gctgagcgtg accgtatcaa tcagcaagcc agtgatttga
     9361 ttcaccgtgt gcaaactgaa ctggacaaaa atcgtaacaa gctaagtaag caagaagctg
     9421 agctgctagc cactgaaaat gctgaattat tccgtcaaaa aggagaatta ttgaccacct
     9481 acctcagtct ggtcccaaac aaccaagact ctgtgatttt agacaattat tatacagggg
     9541 aaaaaattga gattgcctta gacaaggctc tgacaccaaa tcaaaatgct caacgttatt
     9601 ttaaaaaata ccaaaagcta aaagaagctg tcaaacactt atctggattg attgccgata
     9661 ccaagcaaag tattacttac tttgagagcg tggactacaa tttatctcaa gcaagtatcg
     9721 acgacatcga agatattcgc gaagaattat atcaggctgg ctttttgaaa agtcgccaaa
     9781 gagacaaacg ccataaacgt aaaaagcctg agcagtacct ggcttcagat ggaaaaacca
     9841 ttctcatggt gggacgcaat aacttgcaaa atgaagaact cacctttaaa atggctaaaa
     9901 aaggagaact ctggttccat gctaaggata tccctggaag tcatgtcatc atcaaagaca
     9961 atcttgaccc aagtgacgag gttaaaactg atgctgccga actagctgct tattattcga
    10021 aagctaggtt atcaaatctc gttcaggtcg atatgattga agctaaaaaa ttacacaaac
    10081 ctagtggcgc caagccagga tttgtgactt atactggtca aaaaacttta cgggttactc
    10141 ctgaccaagc caaaattctc tctatgaaat tatcctgata agcaactaaa aaacagctct
    10201 cctaggaaag ttatttcccc taggagaact gtttttttat tctgattgtt ggtgcttgat
    10261 atggtcaaga aagtcttgca tattcacatc atcaggcact tgtctgaggt aagcggtagc
    10321 catttggata gccttgtgaa actggccaaa ctcccttaat aaataggcat aatcctgtaa
    10381 aaactccgga ttttctgcca aatcagccga aatctcatta taaagagcca aggcaacttc
    10441 ttcttgttcc aaggcgtggt aagcctttgc aatggtccat ttggttaaga cattatcaat
    10501 cgtctcacga ttaagagcaa taacctcctc aaaacgctcg gcatcaaaat aaagggtcac
    10561 taaacgcatg agaatctctt cgtcatcaac agctacttcc tttgcttgaa gcaggtaatt
    10621 ttcagcattc tgcctatcgt gtaattcata agaaagctgc gatgctagta ataataattg
    10681 actgtcaaat gcatttttac gtaggccctg ttgaaccaag cgcaaagctt ctgacgtctt
    10741 atgctcttca tgaagactca aagcataacc atattcataa cctggataat ctggattgat
    10801 ggtttccaac tgtttaaagt aaagattagc tttttgataa ttttcttggt catacaagag
    10861 ggtcgccaat tcaaaaacag tttcatcttc gtattcaatg gccacggcct tttccaagaa
    10921 ttcgatggct gcctcaaatt tccctaagct agcataggct cgtccaatgc gttgataagt
    10981 ggaaatacct gtcaattcaa ggatttgacg attgtctaac tgtgcatagt aatcaatagc
    11041 ttctttaaaa tactgcaagg acatgtcaat ttctgccaaa ccaaaaatga ccaatggctc
    11101 tgggctgatg ctcactgctt gaagcaattt ttcacgcgcc acctctgtca gtccttccat
    11161 gtcatagagg tcagccatta ctaagagagc tgacaaataa ttggggctat cttttgaaac
    11221 cttatccaaa tacaagaaag cttcttcaat ggcgtcgtct tctgctgcaa tctgagctaa
    11281 atttatattg agttctgggt aatcatcagc taactgcaaa taaatacgct tggcatgtgg
    11341 caaaaaacct atactttcta agtattctcc taaggcgatt aaactatctg catcatcttc
    11401 ttttaaagct ttttggaaat atttttcagc gtgcgctagg tcttgctgat ccaaggaagc
    11461 aatcattttt tcactattta acatggtcct taacctcctc aatcaccaat acttcttcgt
    11521 catacaaacc gctaactttt ttgtaccagt caaataattc tttaatcaca actttgacag
    11581 aagcgtagat tgggatgact aagaagacac cccaaacgcc aaacattgat cctgcagtca
    11641 acaataagaa catgatcgta atcggatgaa tgctcagctt atttccaaga acaagcggtg
    11701 ccacaaaacg gccttcgatg gtttgctcaa tcataaagat aacaagcacc ttaaccaaca
    11761 taaatgggcc ttgtaccata gccataatca caacaggtat catggcaagg aaacttccta
    11821 agtaaggtat catgttaaga aagccagcaa taatcccaaa agtgatggca tatttcagtc
    11881 ccacaagact aaacatgata gaaaacataa agccgacaac aattgcaacc gtaacctgcc
    11941 cttggacata gcctgataat tgcttgttaa tatctcccaa gactcgaacc ataggcaccc
    12001 gtaatttgag aggtaagaca ttcacaaggc catttttcat gtggctactg tctcgtaaaa
    12061 aataaaagag aataaagggc gaaataataa tagccacggt aatccgagca atggcaccag
    12121 caaaattacc agcccagtca atagcatttt ttgaaaaact ttgcgcgtaa tccaaagcct
    12181 tttgacttgt atgggtaagc atgtcttgga gttggggctt gtagctgact aaccattcat
    12241 tttcaagaag tttattggct tcttcattga ccttgcccac atatttgggc agatcctcaa
    12301 taaaactggt aagttgggtc tgtaccatgg gaacaaagcc tgaaatcgcc cagacaaaaa
    12361 gtaaggtaat caaaccaaaa acaataaaaa tagaggtggt acgattcggg cccaagtgat
    12421 taataaggtc aactaagggt ttggtcagat aatacagaat ggtcgaaata accaagggca
    12481 acatgatgac cgcaaaaaaa gaaatcactg gcataaataa aaaagagatt ttagtaaaaa
    12541 caaaaatcgt taaaaaggct aaaagtgtta ttactaaagc catggtagct tgattattta
    12601 aaaaccattt gtaaaataag ctatctgttc tagacttgtc aggtattaag ggcattaaga
    12661 tagggttcct ttctttggga aaatgataag ttttactggt aacctgggta aatcaataaa
    12721 aggtcacaga tttatccatg agttggttgt tttggtcata ggtaaatttg gcagagaaaa
    12781 aaggacggtc ttctaaaatc catttaaaaa tgtcataatc accttcccaa gttggctttt
    12841 ccaacacttg atcgtaaggt acccattcca aggttccttc tcgtgactct tcatcggaaa
    12901 tgaggtctcc ttcaaatcct gttactttaa aaacataggt ataccagtca tgaccagggg
    12961 taaattctgg aaaagtaatg attcctttaa aggccatctc tgtaacagtc aaatgggttt
    13021 cctctaaaat ctcacgacgg gcacactcat ctggggtctc accagcctcg agcttacccc
    13081 caactgaaat ccacttgcct ttgtggacat cattttcttt tttattgcgg tgtaataata
    13141 gcaaagaatc gccattatca atgtaacaaa tggttgctaa ttgagtcatc tatctctttc
    13201 ctttccatct ttgtttctat tataccaatc tctagaaaat atgctatcat tttatgagtc
    13261 aaagtgtgaa tacttagtca cgctcaaaac cttctaaaga tgccttaaaa aagtgtagat
    13321 aagagcctac aatgaaaaac atcaatttaa actatcaaaa gaaccttgtc atatcaacga
    13381 taattgatat gacaaggttc tttaatgttt tatttaatca cttcttgagt tttagcatac
    13441 ttggcttcta cagcatcttt ttctgctttc caccatgtct cattttctgt ataccactta
    13501 atagtttctt ccaaaccttc tgaaaagttt gtaaattgtg gttcccagcc gagttcttca
    13561 cgcaattttg tagaatcaat agcgtaacga agatcgtgac cagcacggtc agttacgtga
    13621 tcataagcgt cttttggttg acccattttc tcaaggataa gctcaagaac ttccttgttg
    13681 tttttctcgc cgtcggcacc aataaggtat gtttcaccga tacgaccctt agtcaaaata
    13741 gcccatactc ctgtagaatg atcattagta tgaatccagt cacggacatt tttcccttca
    13801 ccgtatagtt ttggcttgat gcctgataaa atattggtga tttggcgagg aataaatttc
    13861 tcaatgtgct ggtaaggtcc gtagttattg gaacaatttg aaatggtcgc tttcacaccg
    13921 aaggaacgta cccatgcctt aacgataagg tcagaagctg ccttagttga tgagtaaggt
    13981 gatgatggat tgtattttgt ttcagcagtg aatttttcac ctggtccttc accctgtcct
    14041 ggaaggtctt cacgaagtgg aagatctcca taaacttcat cagttgacac gtggtggaaa
    14101 cggatatcgt atttacgagc tgcttcaagc aaagtgtaag ttccgataaa gtttgtatgg
    14161 ataaatggac ttggatcttc caatgagtta tcgttgtgac tctcagccgc atagtgaaca
    14221 atagcatccg ttttggcagc caatttgtct accaattcag cgtcagcgat atcaccaaca
    14281 actaactcaa cacgatcacc aagaatagct tcaatgttag cacggttacc tgcatatgtc
    14341 aatttatcaa ggacagttac atgaacatct gggtggttat tgtagacata gtgcacaaag
    14401 ttagatccga tgaaaccagc tccaccagtt acgataatat ttttatacat aagatataaa
    14461 gggacctttc gtttccgaaa aaatctatta aaagtaataa ctctaacaaa gacgttttag
    14521 gcctaagaaa acatcgcttt ctaagatttg agtcccatgt tcagctcaaa gagagaaact
    14581 gaactttcct ttcatcattt ttagttgtta cttgttacct gaagtagaga agaataactc
    14641 ccctcatatc cacctcaaat gattaatccc aatcatctga gtatgttgtt tccctatagg
    14701 tcttttggtt tcagtggttt gacatcacta agaagaggat ggtttttatc tgcttcagaa
    14761 acttctgcag ctgctagatt ttcccaagta atcccaagac ttgggtcagc atagtttaca
    14821 aaagcatact taggtttcaa gtcaagagcc cagtagtcat tgacaagata actgtaagag
    14881 acagtctctg aaagaacttg aaagccatta gcaacccctc taggaacaaa cattcctttt
    14941 gaagcatcaa tcactgtctg gtagacgttt ccaaaagtct ctccctcacg aagatcaacc
    15001 caagctccta aaaccttgcc atcatcagca actgagatgt atttatccca aggttcagca
    15061 tgaagtccac gcagcacatg ctgacgtgaa aaagaaacgt tgttttgtag ttttccttcc
    15121 tcaaaaaaac gttctggaaa gccaattggc aacatttttt ctttttggaa attttcttta
    15181 aaccagccac gattatcccc acgaacagga atatcaaact ctagtaagcc tgggatttct
    15241 ttgatttctc ggcaagccaa tggtttgtca aaaaaagttt ctgtcattaa gcttctccaa
    15301 tcaaacgaag caagtattgc ccgtattcgt ttttctttaa agactgcgcc aattcatgga
    15361 catcttcttt actgatgtaa cccatgcgat aggcaatttc ttccagattt gccacttgag
    15421 cgttttgcaa acgttgaact gtttcaatat attgagccgc ttcaagtaga ctttcatgcg
    15481 ttccagtgtc tagccatgca aaaccacgcc ccatcaattc aacggagagg tcaccacgtt
    15541 ccaagtaagc cttgttgacg tctgtaattt ccaactcacc acgcgcgctt ggtttaatgt
    15601 ttttagcaat ttctaccacg tcattgtcat agaaataaag tccagtcact gcaaagtggg
    15661 acttaggcac ttctggtttt tcttcgatag agatagcatt catgttctca tcaaattcca
    15721 ccactccaaa gcgctcaggg tctttcactt gataaccaaa aacggtggct cctttttctt
    15781 tggcagctgc cttttgaagc attttagtga ggccattacc gtggtaaata ttatctccta
    15841 aaataagagc aacacggtca tcaccgataa actcctctcc aataatgaaa gcttgtgcta
    15901 aaccatctgg gcttggttgt tccttatagg ataagctaat gccaaattct gacccatctc
    15961 ctagcaattc ttcgaaacga ggaagatctt ggggggttga aatgattaaa atatctttaa
    16021 tccctgccaa catcaaggtt gacaatgggt aataaatcat tggtttgtca taaatgggca
    16081 tcaactgttt agatgcggca cgggtcagtg gatacaagcg tgtccctgaa cctcctgcaa
    16141 gaataatacc tttcatacgt agtgctccta ttcttctata taccctatca ttctatcatt
    16201 ttttgaaagc agtgtcacgc tttaccagta gaccgcaaaa ggtaaggctc aggggaatag
    16261 tcgctaggcg ttaaaagtcc ttcgtgtcct agcaataatt gagctaattc atagccaatc
    16321 aaagggccaa ctgttaatcc cgatgaccct aaaccactag ctgtataaag gttcttcaat
    16381 cctggaacct gaccataaaa aggagaataa tcactggtat aagctcttat ccctacacga
    16441 catgattttt gagtagcttc ttttaataac ggcaaataag tgagagcttg catttcaagt
    16501 ttttctaaga cctgccaatc aggctccaaa tcataacctt tatcattttc atggctcgca
    16561 ccaacagaaa tcttaccttg attgaaagga atgaggtcta tttcaccttc tggcataaca
    16621 acgggataat tgtcgctgat gatgtgatga acgtcataat ccaagagttg tcccttttgt
    16681 ggtcgcacat caacctgata gcctaaaggc atcaataagt caggtagcca tgctcctgct
    16741 gccagaatga cctgatcaaa ataatgacca gcaatctcat aaccacttcc ttgctgtttc
    16801 agagtgactt tttggcgaat aactggataa ccactagtct tgagtaacgt ctcacataaa
    16861 gcggctccct ccactctagc agctcctgaa gcgtacagac agttatcaaa gccaatcaat
    16921 cctttaaaat catcccccag ttctttacga tttttaatgg ctaactcacc aataatcggt
    16981 gattctacct ttcttgctaa cgctaattca taaagatctc ttagtttttc ttcctgcttt
    17041 tttaaaacat agatgccatt ttgctgataa aaatctgttg caaaaccatc ttctttgagg
    17101 tcattgatta attgctgata aaagtccgct cctaaacgag ccatgcggta ccaaacttta
    17161 ttgcgacgct ttgaaaacca aggactaata ataccagcag cagccttagt ggcttgtcct
    17221 tgaccatggt caaaaatggt aacttctttt tgaccacttt gttgtaaata ataagcggcg
    17281 gttgatccta caatccccgc cccaataatg gctattttca ttcctggtat ccttttatct
    17341 tactttaaga ccttaacagc ctataaatgg ctaaatggat tggtcgaggc tttgcttgaa
    17401 ataatgctga catcccaacc gttctcttct ttccaccctt ggagtttctc ctttaatttc
    17461 tccgtaaaaa gcacctcaat atgatgcccc gggtcaaccg caaatagtcc ttctgtcagc
    17521 atctcctgtg ctgtatggta atagatatcc cctgtaatat aaacgtctgc gcctttttgc
    17581 acagcatcct gataaaattc accaccactt ccgccacaga tagcaatctt tgaaatcaaa
    17641 gggttttcct tatcgtaacg gataagtcgc acggtatcta aatcaaacac ccttttgacc
    17701 ttacttgcta actcttctaa cgcttgttct ttaactgttc caatacgacc aatgccaaag
    17761 ccttctttgg tttcagacaa gtaagttgct tccttgattt ctagcaggtc acaaaaccaa
    17821 tcatttagcc cgcctggtac aatgtcaata ttggtatggc tcacatagac agaaatgtca
    17881 tgcttgacca aatctagaag gatatcacgc tgaggggatg aaaccagatc tttaagcggt
    17941 ttaaagatgg gagcatgctt agttataatg agatcaactt catttttgat ggcttctgct
    18001 actgttgact ctcggatatc taaagtaatc ataaccttac gaatgtcttt atccaatgaa
    18061 cccatttgaa gacctttaac gtctctttcc atagataaat caagtgggca aaaggcttca
    18121 taggcatcaa ttagggtttt agctttcatg aacaatcacc tcttttattt gttgtatttt
    18181 tgtcaagaga agctgacgct cttgagtttt ttcttctggg atgcaggaca aggcataagc
    18241 taacttatcc atttctcgtt gccacttctc tttaaaaacg actgattttt cctgagaaag
    18301 gtatggacca aaacgcaatt ctgtcgcgga taaggccttt tcaccatgct cagcaacgat
    18361 gatttcataa tatttgtcat tttcagccat gatggtttct gcaacaattt taaaagcgtt
    18421 aaccgataac caagcccgta aatcatcctc acgattgttg ggctgtaaaa ccagacgttc
    18481 aatgccctgc aatttttcct tacctgcctc taaaatatca gtaatcaaac ggccacccat
    18541 gccacaaatg gtaatggctg tcacatcatc agcttcttca aaggctgcaa ggccattggc
    18601 taagcgcacc tgaatatgct cggcaaggcc agactgtgtg acatttttaa gagcagattc
    18661 gtagggaccc ctaaccactt cgccagcaat ggctgctgaa atctgattag tttctaccaa
    18721 aaatatggga agataggcat gatcactgcc aacatctaat aatttgacac cttttggaac
    18781 ataggccgca acttgggcta aacggtttga taattgacta tccatcttga tttctttcta
    18841 aaacgtaatg gttctattat agcaaaatta cctccataaa aaaagaaaac caaaggctag
    18901 gctttcgatt ttccataagt attttgatag tacttacagt aagactgaac gggacaaatc
    18961 tcacattttg gttttttggc taaacaatgg tagcgtccaa agaaaatcaa tcgatggtga
    19021 gtaataatcc aatctttctt tggaatttta gccatcaaat ctgcttcaat ctgcttgaca
    19081 tcggcatctg gtgatgaaat attgagcctc tttgagactc tagccacatg agtatcaacc
    19141 gcaatagctg gaacaccata aacctctgcc aataccacat tggctgtttt tcggccaaca
    19201 cccggtaaac tttcaagctc tttgtgggtt tttggcactt gacccttaaa atcatcacga
    19261 atagcctgag cagttttaat gatatttttg gccttatttt tatagagtcc aatcgttctc
    19321 agagcatttt caacatcaga aagttcagca aaagctaagt cttctatttc tggataagac
    19381 tgccatagtc caggagttac cttattgacg gccttatctg tcgtttgagc tgataaaatc
    19441 accgcaatta acaattgaaa aggtgtttcc cagtctaact ctcccttagc ctcaggaaac
    19501 atttgaccaa tgatggtcag gacttttgct aatcttgctt ttccaattct catgaatcac
    19561 tccataaatt catcgcagat agaaaatcct ctgatatcgt cacttgagaa agatcttcgc
    19621 cttctctaac acgtctacgt tcttctactt gtcgaagatt aacaatacct tcttttcgcc
    19681 aattacgtaa gatagcctgg atatatttcc aatttgtttt gccgttgaag acagcttctt
    19741 ttaaagcttc tcgtatcaaa tcaggatctg ttttatcatc acgaagcgtt ttttcaagat
    19801 cttctaactc aaaaggactc aagaatcgtc ctaattcacg ttcaaattca tcaactaatc
    19861 gtttgaaatg gtttggtgtt tcttgtttgt caatttcagt tgccgtttga ctgacaaaca
    19921 attgatctaa cttggctaac acaggactcg catcaaaaat aatttcaatt tctccagtaa
    19981 gttcaattgt ccgcatatct aaaagccctt gactagtcaa agatgaaatg attttattaa
    20041 tatcagcaac tgattttcca agcgcatgag caatttgact gggcgctaag tcgtctcgct
    20101 ttgtcgtatt ttgaagatag aaaaactgcc agaccaaaaa atcatctgag ctcttaaaaa
    20161 gatctttgta atgaaaaagc aaggcacttg ggataaccaa attacctgac ttataatgtt
    20221 ctaaaaaact cataatccct caatcaaaag gctaaaatcg aatctatagc ctttactact
    20281 ttaaatcccc taatcagatg ctgattaatg tgcttaccca ggaaactgca ttaagacttt
    20341 atagtcatag ttcctaatag catgacgacc atttaaacct tctaattcaa taaggaaggc
    20401 acaaccagct acaataccac caagtttttc aatcatttca atagttgctt ttaccgtacc
    20461 gccagtcgct agtaaatcat cgacaatcaa aacacgttgt ccaggcttaa tagcatctgc
    20521 atgcatagtt aaggtatcaa gaccatattc tttttcgtaa tcggcagaga caacatctct
    20581 tggcagtttt cctggtttac gaacgggagc aaaaccaatt cctaactcaa ctgcaacagg
    20641 acaaccaatg ataaaaccac gcgcttcagg tccaacaacc atatcaatat ctttatcaca
    20701 agcgtactgt gctatttcac gaatagcata gctatatgct ttaccgtcag ccattaaagg
    20761 tgagatatct cgaaacgtaa taccagcttt agggtaatct ttgatcgatg caatgtaatt
    20821 tgttaaatcc atagttaatt ctttctagtg gcaaattgcc aaatataaaa tactcttcat
    20881 tatatcacga aacatatttt tcgtaaaata agacttctga cattatgttt atctgatact
    20941 tcattcgtaa ctacctcaat aactactgtc aactgcttta cttattgatc gtttgatttc
    21001 accaaataat catacatctc tttcgggcta gccaaagcca tgatttcttg aaatttcacc
    21061 aattctttta agtcttggta aatgtggctc tctgaaatct ctcgtttttg cgcttggggg
    21121 ttaaccgtca tcagcccatc gtcaatcgtc acaaaagaca actcttcaaa aatttgaatc
    21181 aacttaatca gtaaaagttt ttctatgttt aaatagtgac tcaactctgt caatttatgc
    21241 cgtaaatcaa attcaggaaa ctgatagatg gttttgtaga gcttggcaaa ctgctctctg
    21301 ctgccaaatc ctgttaaata ataaggatgt ttcatctgat ttttaaaata gatggcgtca
    21361 aaatccttgt gaacaaactg gttgcgccag gttttgaaat cttcagggat gtcattaatc
    21421 agaatgactc tggcatttgg gtcttcttca atagtcggaa taccttcagg aactttggct
    21481 gtttttgtcc ttaagtcaag aagttgcact ccgtcaacac gagcatctac taacatgaat
    21541 tggagactcg tgttcccatt ccaatggttg acagataagg taactgctaa ttctaaacca
    21601 gtagcttgtc ggaactcttg cagttgactt ccttgaccaa aagccagtac gtcaaaggaa
    21661 gctttgccct gactgacttt aaatttaaga tggctctggt cttgccctat ggtacgcgct
    21721 tgactaaccc ttatgtcctt gacataaaag accggctttt gatgatccat cccataaggg
    21781 gctaatttat ctaaactctt taaaatatca aggctaaggt cgtccaaaga cagtctttca
    21841 tcaatagtta acgtattttt ggcagtctga tctaagccac gttctatcac aaactgacaa
    21901 agaaagtctg aaagagcttc caagttgtca acgggcaggg tcattcctgc tgcaccagca
    21961 tgtcccccaa aggctgtaaa taactcacgc ttgccattca aggcttcaaa aatattaata
    22021 gcttctaaac ttctggccga gccttttgca aagccattat caatggtcaa aacgacgacc
    22081 gtttgtccaa tagtttccat gatacgacca gcgacaatcc caagaacacc aggatgccag
    22141 ccagcttgtg ccaagacttg aacaggtttt tgaggatcaa ccatagccat cgcttgatca
    22201 aaaatgtctt ggactaaggc cttacgctct tcattttttt tcttaatcat caaggcaatc
    22261 gcttgagctt cttggtcatc aaagcccgtc agtagctcaa tggctggatt agggtcatct
    22321 aagcgcccta aagcatttaa ttgcggagca atctgaaagc caatagcgtc ttcattaaaa
    22381 tgttccaaat caataggaga caaagacatc aactcctgca agcccactct ttcggtgtgt
    22441 ttgagcatgg ccagaccatt tttgactaaa acacggtttt cacctgtcaa gcttaccata
    22501 tcggcaatgg ttccaatagc taccaagtct agacagtctg ttggtaaact ttctagcaaa
    22561 gctgttgcca atttaaaggc aacgccacag ccagctaagt gtttaaaggg ataatcagca
    22621 tcaggatgct caggatgaat gatggcaaag gcttctggca attcttcagg caggctatga
    22681 tggtcagtta caatgacatc aacttcctgt tcttgagcat atctaatcgc ttcatgtccg
    22741 gccacgccat tatcaaccgt gataattagg gacacagctt cctgctcaat aaaatattta
    22801 tagacgctct ggttggggcc atagccatct gtaaagcggt tgggaagata aaccaaaggc
    22861 tctgttccca tcatgtctag ggtttctttg acaatagaag ccgacgtcat gccatcagca
    22921 tcatagtcgc cgtaaatgag aattcgttcg ccctcttcaa tagcttgacg aattcttggc
    22981 acagcttttg ccatgtcatg tagcaagtaa ggatcatgca gttgagacaa gtccgctgtt
    23041 aaaaactcat ctaaggcttc ttcagtcctt atccctcgct cataaatcaa ctgagcggcg
    23101 gtctgggtta gaccttttgt ctttgctagc ttaaagaagc catcatctgg cttcttgtct
    23161 tttattttcc agctatattt ggatttaatc atttataatt aaataccttt cttgcaagat
    23221 aatcagataa tttagggaaa agggtgtaaa attgatgggt caccgctaaa ctaaagggca
    23281 aattcaattc tcgtttattt ttcccgataa tagaaaccaa acgcttagcc acttgatttg
    23341 gttggagagt aaatgtacca acgctttcca aataatgtcc agacgggtca gcttggtcaa
    23401 aaaacttggt ggcaatggga cctggattca cggtggtcac gtaaacccct ttatccgcta
    23461 attctaagcg aagggcattg gaaaatccga taagggcaaa cttggtggct gaataaatgc
    23521 tcgatttggc tgacgcaatc aagcctgcca tggacacaat attaataagg tgaccttgcc
    23581 cctgctctgc cattttctga ccaatcaagc aagcaaagtg aatgctcgct agggtgttaa
    23641 cctgaaacat atcagctatt tcttgggcag aaaactcttc aaagcctttg aaagctccgt
    23701 agccagcatt attaatcaag acatcaatac ggccatagcg ctggtaaatc tgggcgacca
    23761 ttttctcaat ggctactgga ttggtaatat ccaactcaag gcattctttg ttgtcaatat
    23821 gctgataaca gtgttctagg cgttctttgt tacgtcctaa taaaatcaag ctgtcttcct
    23881 tgggtaactg cttaacaatt gcctgagcca gtcctccaga agctcccgtg ataacaatga
    23941 ttctttgtgc cattaaataa tcacttcctc taaatcttgt accattttaa cattttcaaa
    24001 tatagtggcg gcatcttttt ccatctggcg acaatcacgc cctaaaaagc gagctgatac
    24061 atgattgagc aagaggcgtt ttgcgcctgc ttcatgagcg atttgtgctg cctgcatgtt
    24121 ggtagaatgc ccgtgatttc tcgcaatccg ctcgtcaccc ttaccatagg tggattcatg
    24181 aaccaaaaca tctgcatctt tagcgagttt aacactggca gacgttttgc gcgtatcgcc
    24241 gataatggtg atgattttgc cttttttagg ggcagaaata taatctttgg cacaaatcag
    24301 gcggccgtct tccaactcaa catcttgacc atttttaatt ttgccaaaga gtggtccgaa
    24361 gggaacaccc gctgctttta aggcttctgc gtctagggtt ccttctaagt ctttttgaac
    24421 tactcgataa cccatgcaaa aaatggtatg cgccagacgc tctgcataga ccacaaactt
    24481 atctgtttcc attattttac ctaatgattt gtcatcaaat tcatgaaaat gaatctgata
    24541 aggtacacgc gctccagaaa cttttagact agttaacacg taagttttaa tgccaattgg
    24601 tccataaata tcaagatctg tctgttcttc actagcttga aaagaccgac ttgataaaaa
    24661 tcctggtaat ccaaaaatgt ggtccccatg caaatgagta atgaaaatct ttcttatttt
    24721 tcgtggctta atcgtcgttt ctaaaatttg tctttgcgtt ccttcaccgc aatcaaacat
    24781 ccagacttca ttgatttcgt ctagtaattt caaggcaaga cttgacacat tgcgctgctt
    24841 ggctggttga ccagctccag ttcctaaaaa ttgtaattcc attcaatcta ttctttctat
    24901 tgggtgatgt aaatcataaa ttctcgttgg gcaaaatcat aatacttttg accagaaaat
    24961 gtttggctaa gagcccataa ctcctctaca ttaaacccct taagcatggt gataccgttt
    25021 gccagacggt aaatcttcga caagagtgtg tcatcaggcg taatgtcttc acattctcct
    25081 tgctcaaact gcttagcctg cattttaagc tggcctttgt cttcataaac catatattgt
    25141 gggaaaattt gtgttaattc aaagaagagt tcgggtttct taggctctgc tttttcagaa
    25201 aataccaatg caacttcttg gtcattttgg taagcaaaac cataagcttt tccagacaaa
    25261 ttgaggcgta caaagggctt ttcttcctca aaagatggtt ggtcagtaaa aagccccaaa
    25321 gccttagaaa gattaaaata ataatcagtc gcagcttgcg gtgactgttt ctggtagatt
    25381 tcagcaaagt aggcattaat aacacttggt ggtggtgtga taaaagctag tttttgctgg
    25441 tctgtcaaac tggctaatag atggtttagg tagttggtat ccagactagt aaatccacca
    25501 taggtttttg ctaaatcaat ataattattc ataaaaatcg tcaagtctcc attgctgttt
    25561 tggggaaatg tagcccgaaa tatcctctat ttcttggtca aacgtgtagt ggtctaaaag
    25621 agcaactcgg ttcaaatcat acagcttgta cgccttgtct tgatgcactt tgattctaaa
    25681 aggaacaaac tggtctcgaa tttggtcaat gattaaacgt ctcaagagaa ttttgctatc
    25741 ctttgaacgg gctgaaatcc taatatttgg aaaagcagta gctgtgaatt gttctgctat
    25801 atccaccttg ttataaatag ctaaacgtgg aatattaagc atgtctagct cttttagaag
    25861 gtttaaaacc actttttctt gctcactatg attagggtcg ctagcgtcaa tcacatgtaa
    25921 cagcaaatcc acatatttgc tttcttctag ggttgattta aaagcagcca ctaattccgt
    25981 tggcaagtct tgaataaagc ctacggtgtc tgttaaggtt gcttgaaatt gattttctaa
    26041 atagagttgc tttgtagttg catctaaggt agcaaataat tcattggctt catagtggct
    26101 gtcatctgtc aacaagttca taatggttga cttgccagca ttggtgtagc caatcaagcc
    26161 aattttaaag gtatctgaac ccacacgacg atctctaatt gtttgacgat ttttctctac
    26221 ctgagttaat tggcgttcaa tatcagcaat ctgatgacga atcgaacggc gatttaactc
    26281 tagttggctt tcacctggtc cacggctccc aataccacct gcttgacgac ttaacatgat
    26341 gccctgtcca accaaacgag gtaacatata tttcaattgg gcaaggtgta cttgtaattt
    26401 gccctcatgg cttctggcac gcatggcaaa aatatctaag attaactgca tacggtcaat
    26461 gacttttacc tctaaaacag cttctagatt agcattttgc ctagcagtca accgattgtt
    26521 cacaataact gcatcaatct catcagcttc cacaatcgcc ttaatctcat ccagtttccc
    26581 tgagccgata aaggttttgc tgtcgtaacg ttctcgtttt tggctgaaac ttgccataac
    26641 tttgacgcca gctgttttgg caagattagc taattctgtc atggacatat caaaatgttc
    26701 tgttgtctga agctcaactc ctaaaagaat cactctttct tgctgacgct ttgtctcaat
    26761 catcaccttt accaatcctc caaattccgt aaattctatt ataacatatc ttcaacagat
    26821 tctgctaatt ttgcgtcatg acttctcttt ctgaccaaga aaatccctga ccctatcatg
    26881 tactacctgt gggtaatcag gagccgttat ggcagtgaag ctaacagcca ttcgattgcg
    26941 gaaccaagtt agctgtcgct tggcaaaacg tctcgtgttt tgttttaact gatcactagc
    27001 ttctgccaaa gtcatttcac ctacaaaata ggggaataat tccttataac ctatgccacg
    27061 actagcctga acagtcgggt aatgctcata taaccatttt gcttcttcca ataagccgtt
    27121 ttcaagcata cgatccacgc gctgattgat acggtcatag atgacttggc ggtcatcatt
    27181 caagccgata ataagcggct cataagccgt ctcggcattc tctaattcat cagcaaactg
    27241 agccaattca agggctcgga tcgcccgcct gcgattcacc tgctcaatgg taatgttatt
    27301 gacttgcaag cgttcataca agtcgtgatc gtccaattgc tccaactcat tccgataggc
    27361 ttttaccgcc tcttgatcaa cttgacctcc tagatgataa ccttcgagta aactttgtaa
    27421 gtaaagacca gttccaccta caataattgg taatttccca cgactaacaa tgtctgaaat
    27481 agctttttga gcatcttgaa caaaatcgta ggccgaataa gactccgtca cctcacgaat
    27541 atcagttaag tgatgaaccg ctgcttcttg ctcttcttga gtggccttgg ctgttccaat
    27601 atccaactgt cgataaactt gctggctatc tcctgaaata atttctccat taaaagcctt
    27661 ggctaaacta atcccaagtg ctgtttttcc aaccgctgtc ggaccaacaa tcacaactat
    27721 ttttattttt gtcattttct tgtctttcta ttagaaaagc gtgtccaaaa acgcctttta
    27781 cttgaaaatt agcttatttt tcgctaccat atattataac ataagattaa ggaccactga
    27841 ggtccttcac tagaaatgga gaataatcat ggaatatcaa tttgttaaag gtcttgctac
    27901 tggcgtccta gcaactgctg ctactgttgc tggtgctgtc tttgctgtaa aaaaaacaat
    27961 cattgaccct gaagaagaaa aagctgcttt cattgaagaa aaccgcaaaa aagctgcgcg
    28021 tcgtcgtgta gctcgctaac gtgacaatgg atcaatttaa aagagactag cacattaaag
    28081 tgccggcctc ttttttagta atctcgataa ctagttagta gtaactgtct cttgatgagc
    28141 caagatgtta gctgctttac ttattttgaa atagaaatct caggtaagcc ccataaacat
    28201 acgtgactaa agctgtcgcc aacaatacct ctcctagcaa aagaacctgc caacctaaac
    28261 cttgccaacc taaaaaagat aaggtcattt ttaaggaagt ggcactaatc acaaacggaa
    28321 aggtaaaagc tgaaaaacct gggttaaagg gacgattgag taagcgagga agctgtacaa
    28381 ccacaaaagc atataacagt tgtgagctga tgagaagaaa gataaccatt cctacttgtg
    28441 gtctaggaaa ggttgctaaa taagacgctg aaagtaagga cagaggggca caaaacgttg
    28501 aaatattggg cattactgct tgacctaaac caatcccata agtctttttg gccataaaag
    28561 gtaaaatcac agcagttaag agaagacaaa cccaaaaaat cacctgtcct aataaaaact
    28621 gcctactagc aggtgctgtt aatgccgcca ttgcaattcc aacaaaaagc acggaccagg
    28681 aaggaaaaac gttatcccat gaaaaagaaa agacaaaacg atactggtaa taagcaatca
    28741 ggacaagatt tcccaaaaag aatagccacc aagtgagaaa gcctaaccaa caccctcctg
    28801 tcactttgag aaagagtgaa gacaacaaca tccctgtcat aaaaaaagtt ggaaaaacag
    28861 aggcaattag aggttgtcgt aactgcattt tggtatcgtt aaggttccta accatgccaa
    28921 caagcaagat accataaata aaaagagctg ccagaattcc caaataacta aaaatcgaaa
    28981 cataagtagc caacaaattg ccaaatgaca acgttcctaa tgccaatcct gacatgacca
    29041 atggtgggtt tttaaggtgt ttcataagtg tccttttctg cctattaaca gctattttta
    29101 gtgacataat cttgtttatt ttatcacatt tcgagcattt ttgccataaa ggctgccaga
    29161 tgcgcacgcc gtacgcatga aaactttctc ttcatccttt tgtgcccgga catcaatttt
    29221 gaaaagattc ctttttaaat caatcatctg cttagcctct
//
LOCUS       DATUYR010000019        29076 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.019, whole genome shotgun sequence.
ACCESSION   DATUYR010000019 DATUYR010000000
VERSION     DATUYR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29076
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.019"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..299
                     /gene="gpsB"
                     /locus_tag="VCI33_000762"
     CDS             <1..299
                     /gene="gpsB"
                     /locus_tag="VCI33_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEP2831698.1"
                     /translation="IFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNAQIEALKAENE
                     ALKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVFGKQIIE"
     gene            313..681
                     /gene="rnpB"
                     /locus_tag="VCI33_000763"
     ncRNA           313..681
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VCI33_000763"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            744..1898
                     /locus_tag="VCI33_000764"
     CDS             744..1898
                     /locus_tag="VCI33_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEP2831699.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWGNYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            1912..3381
                     /locus_tag="VCI33_000765"
     CDS             1912..3381
                     /locus_tag="VCI33_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831700.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERGIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGYQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(3527..4009)
                     /locus_tag="VCI33_000766"
     CDS             complement(3527..4009)
                     /locus_tag="VCI33_000766"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEP2831701.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(4083..5414)
                     /locus_tag="VCI33_000767"
     CDS             complement(4083..5414)
                     /locus_tag="VCI33_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEP2831702.1"
                     /translation="MEIIGSLGVLIGVIVIIYLSVKEVNIIIAAPLATSLVILFNQMD
                     PTTTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGETTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMARG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIMIAFDLFS"
     gene            complement(5485..6264)
                     /locus_tag="VCI33_000768"
     CDS             complement(5485..6264)
                     /locus_tag="VCI33_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEP2831703.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETFLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(6297..6956)
                     /locus_tag="VCI33_000769"
     CDS             complement(6297..6956)
                     /locus_tag="VCI33_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEP2831704.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(6958..7608)
                     /locus_tag="VCI33_000770"
     CDS             complement(6958..7608)
                     /locus_tag="VCI33_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEP2831705.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDTLVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITVDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREIVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(7632..8819)
                     /locus_tag="VCI33_000771"
     CDS             complement(7632..8819)
                     /locus_tag="VCI33_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP2831706.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            9020..9916
                     /locus_tag="VCI33_000772"
     CDS             9020..9916
                     /locus_tag="VCI33_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP2831707.1"
                     /translation="MDIKQIRYFIAIVENHFNLSQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSISIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNVKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQFKDIKCILMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
     gene            10046..11653
                     /locus_tag="VCI33_000773"
     CDS             10046..11653
                     /locus_tag="VCI33_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEP2831708.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            11763..12398
                     /gene="gmk"
                     /locus_tag="VCI33_000774"
     CDS             11763..12398
                     /gene="gmk"
                     /locus_tag="VCI33_000774"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEP2831709.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            12414..12731
                     /locus_tag="VCI33_000775"
     CDS             12414..12731
                     /locus_tag="VCI33_000775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEP2831710.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            12796..15180
                     /locus_tag="VCI33_000776"
     CDS             12796..15180
                     /locus_tag="VCI33_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEP2831711.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKGIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTKRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            15242..16177
                     /locus_tag="VCI33_000777"
     CDS             15242..16177
                     /locus_tag="VCI33_000777"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEP2831712.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMELGADGIITAAFGQFLPTILLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSD
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIAAGQGA
                     LSLLVVQPAGKPKMAIADFLNGLGRKLEVGDFIGR"
     gene            16167..17489
                     /gene="rsmB"
                     /locus_tag="VCI33_000778"
     CDS             16167..17489
                     /gene="rsmB"
                     /locus_tag="VCI33_000778"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEP2831713.1"
                     /translation="MADNWKISIRGKALLTLEHIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRSVAIMESLFVRSKASIRVTDPLKLEEAAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            17527..18267
                     /locus_tag="VCI33_000779"
     CDS             17527..18267
                     /locus_tag="VCI33_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEP2831714.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            18264..20162
                     /gene="pknB"
                     /locus_tag="VCI33_000780"
     CDS             18264..20162
                     /gene="pknB"
                     /locus_tag="VCI33_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEP2831715.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            20285..20977
                     /locus_tag="VCI33_000781"
     CDS             20285..20977
                     /locus_tag="VCI33_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEP2831716.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            20974..21978
                     /locus_tag="VCI33_000782"
     CDS             20974..21978
                     /locus_tag="VCI33_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2831717.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            21971..22612
                     /locus_tag="VCI33_000783"
     CDS             21971..22612
                     /locus_tag="VCI33_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2831718.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            22649..24049
                     /locus_tag="VCI33_000784"
     CDS             22649..24049
                     /locus_tag="VCI33_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEP2831719.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPLLQEKADFITKKVKEDGILYALEELGLIDKELQFPQL
                     DLPNHKGPKATIKTNHGDMTLVLFPDHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPAPIAASYAAIGGTPHLDRRHTVFGQLVDETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            24049..24426
                     /locus_tag="VCI33_000785"
     CDS             24049..24426
                     /locus_tag="VCI33_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP2831720.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALENGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKPSLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(24444..25385)
                     /gene="cysK"
                     /locus_tag="VCI33_000786"
     CDS             complement(24444..25385)
                     /gene="cysK"
                     /locus_tag="VCI33_000786"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEP2831721.1"
                     /translation="MTKIYKTITELVGQTPIIKLNCLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETVIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(25513..26145)
                     /locus_tag="VCI33_000787"
     CDS             complement(25513..26145)
                     /locus_tag="VCI33_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEP2831722.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            26201..27526
                     /locus_tag="VCI33_000788"
     CDS             26201..27526
                     /locus_tag="VCI33_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2831723.1"
                     /translation="MESIENSYGRLLLESQLPDSAKQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPNGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFNNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN"
     gene            27498..28163
                     /locus_tag="VCI33_000789"
     CDS             27498..28163
                     /locus_tag="VCI33_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEP2831724.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            28243..28791
                     /gene="raiA"
                     /locus_tag="VCI33_000790"
     CDS             28243..28791
                     /gene="raiA"
                     /locus_tag="VCI33_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEP2831725.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT         9386 a         5176 c         5933 g         8581 t
ORIGIN      
        1 atatttttga gcaagaattt aagactagta tgcgagggtt tgataagaaa gaagttgatg
       61 aatttcttga taatgtaatt aaagattatg aaaatttcaa tgctcaaatt gaagctttaa
      121 aagctgagaa cgaagcctta aaaaaggcca agtttcaagc tagaaataca gtttcagcga
      181 ctgttcaaca accagtaccg caaccgactc gtgttgctca atcagctact aattttgaca
      241 tcttgaaacg tattagtaaa ttggaaaaag aggtttttgg taaacaaatt attgagtaat
      301 gaaaactgtg caatttttgg ataatcgcgt agtattttaa tactatgagg aaagtccatg
      361 ctagcactgg ctgtgatgcc agtagtgatt gtgctaggcg aacacataag cctagggatg
      421 tgcatacaca ttacggcgaa ggaaatggct aagtcgtttg atatgccaaa gtacttctga
      481 aagtgccaca gtgacgtagt ttttatggaa acgtaaaaaa tggaacgcgg taaacccctc
      541 aagctagcaa cccaaacttt ggtaggggca tgggatagtt ggaaacgaac aagctatcct
      601 gactgtcaac agacggtaga cagatgatta tcgaaggaaa taattcctag ttatttccgg
      661 aacaaaacat ggcttataga aaattgcata ataggttaag ctagcacttg ctagctttta
      721 tttgatttaa aagaaagaga tatatgaaag aaacatttag attagtcgca actgcagccg
      781 caggtttaga agctgtcgtt ggtaaagaag tcagagcact aggttttgat tgtcaggttg
      841 aaaatgggaa agtttatttt gaaggtgatg tagaagcgat tgttaaaaca aatctttggt
      901 tacgtgcagc agatcgtatc aaaattatcg taggacaatt tcctgctaga acctttgaag
      961 aattattcca aggtgtcttt gccttggatt ggggaaatta tttaccgcta ggagctaagt
     1021 ttccaatctc aaaagccaag tgtgtcaagt caaagctaca caatgagcca agtgttcagg
     1081 cgattaccaa aaaagctgtt gttaagaaac tgcaaaaaca tttccacagg ccagaaggag
     1141 ttcctcttca agaagttggg tcaacgttta acattgaggt atctatttta aaagaccaag
     1201 ccactattat gattgacacg acaggatcta gcctatttaa acgagggtac cgcgttcaaa
     1261 agggtggggc tcctattaaa gaaaatatgg cagctgctat cttggcctta tctaactggt
     1321 ttccagataa acctctggtt gatcccacct gtggatcggg aacgttttgc atcgaggcag
     1381 ccctgattgg gatgaatatt gctccaggat ttaaccgttc gtttgctttt gaagaatgga
     1441 gctgggtaga taaagacatg gtgcaacaag ttcgtgatga tgccgaacaa gaagctaatt
     1501 atgaaatcga attagatatt tctggctttg atatcgatgg ccgcatgatt gagattgcta
     1561 aaagtaatgc tgaagaagct ggtttatctg atgtgattac ctttaagcaa atgcgtctgc
     1621 aagactttcg aacagacaag gttaatggtg tagtgatatc aaatcctcct tatggagaac
     1681 ggttgcttga tgacaaagcg gttgacatct tgtataatga aatgggacaa acctttgcac
     1741 cattgaaaac atggagcaaa tttatcctta caagtgatga actttttgaa ctaaaatacg
     1801 ggcaaaaagc tgataaaaaa cgtaaacttt ataacggcac cctaaaagtt gatttgtacc
     1861 aattttatgg ggaaagggta aagcgacatc tgatatagag aggtagtaag cgtgtcagaa
     1921 gaaagcaaag aagtcgaagt aacaaaagaa tcgcaaacat tggggcttaa tgaagccaag
     1981 tcaatgacaa ttggtgaagc tgtacgcaaa cagtcagaga taaaagcagg tgttactaag
     2041 gatgatagta ttttagataa gtacattaag caacaccgtg acgaagtttc ttctcaaaag
     2101 tttgatgcta agtatacaga gcttgataca gctagtttag ataactttat caaaaaacaa
     2161 agagaagcat tgagtaaagc cggtcttgtt gatgacgaac ctgtgtctgc ggagtctgct
     2221 gagcaagata gcactttggt tgaagaagta gctgaagact tagctccaat ggagactact
     2281 gcggtggtaa ctgggattcc ggtggaagcg acagtccctg tgttagatct agatccatca
     2341 gagaggggca ttccagaacc acagatgacc aaggaagagc caaaaagaga tcagtttctt
     2401 tctgaagata gccaccatcc agccaaacaa aacacgaaaa aaggttggtt aatagcttta
     2461 tttctactat tattggccat tcttgcagtt gtatttggtt ggaaccattt cttgagacaa
     2521 gacagtggta aaacaactca aacagcaagt aaacagacaa agacctcttt gcagaccgac
     2581 agtgctaaaa aagcaacgcg cttgaaggct gctgctaaag ctttcgaaaa acaatatggt
     2641 actttttata cagatgccac aaagagcaaa ttaaaaaata gtgcctttgc tactttgcca
     2701 gatttagaag ctgccttgaa agctttggaa ggctctgctt actatgataa ggcgaaagca
     2761 aaagtcgatt cccttaagaa agctattgct gctattacag cggttaacgg taaatttgtt
     2821 tccgatgttg ttgtagatgg tgaaaaggtt tcggctgaag tcaaggctga tgctaatttt
     2881 gatgatttat caagtgcaac cttgacaaca ggaaatgcaa atcttgatgc tgtcttgcag
     2941 gcaagcatca cagaaggtcg tcagcaacta gccagcaagg ctgaggccgc taaagcagct
     3001 aatgagcaag cagcccaggc tcaagcagct caaggacaaa gtacatcagt agcaccatct
     3061 ggctatggct tgacaagtta tgaccctgca agcttacaac ggcatttatc acgtgtacct
     3121 tacaatcaag atgttattgc tgacagagct aacccatctt gggcatttaa tccaggagtt
     3181 cttgaaaaaa ttgtagcaac ctcacaagct agaggttata tttcaggcta ccaatatatt
     3241 ttagagccgg ttaatattat taatggcaat ggttactata atatgtttaa gcctgatggc
     3301 acttatctgt tctctattaa ttgtaagacg ggttattttg taggaaatgg caaaggttat
     3361 gctgatgctt tagattactg agaaaatcaa aaaaaactga gataaggcaa cttgtctcag
     3421 ttttttgatt aataaagtat gttgtcttaa aatctgcaga tagacttgtt ttttgtaaca
     3481 agtcaaatag gtcagtagga ttgttatgtt tgacaacaca ctagtatcag atgacatggc
     3541 gactaaaagg atcgtctgaa atcccttgat caataatcaa ttgagcccat tctttggcgg
     3601 caaagaggct atgatcctta tagtttccac aggattcaag agttgttcca ggaacatctt
     3661 cccaagtaat cccagttgca atttcttcta ggctggattt aatcaccttg gcaatatcag
     3721 tagaactgtg ttttccccac atgataaggt gaaaacctgt tcgacagcca aaaggagagc
     3781 aatcaatcat cccatcaatg cgttggcgga tgagcttggc aagtaagtgt tcaatggtat
     3841 gcaaaccggc tgtttcaata gaattttggt taggctgcac taggcgaaca tcaaaatttg
     3901 taatacgatc gcccttgggt ccaaattctt cagaaataag acgaacataa ggggccttta
     3961 caatagtatg atctagctca aaactttcga cgataacttc ttttgtcatt tcaatctcct
     4021 ttgttttaca ctttatcata acacaaatag tgagaaagtg gctacttgtg gaggagaaaa
     4081 ggctaagaaa acagatcaaa ggctatcata atgacctgtg cgataaaagt acttacagaa
     4141 acagtaataa aggtttcttt aaaagcattt ttgtggttaa gtcctgtcaa tgctaagaag
     4201 gtgagaaaga caccactttg gggcacaatg gtaaagatat tagaagcaat agtggcaaca
     4261 cggtgaatca tttcgggatt aaggccttga tctaaataat actgggcaaa gttaggcatg
     4321 acaattccta gagcaccgga agaagagccg gtaatagctg acatagagga agtcaacacg
     4381 gctaaactaa taagaggatt accgggaata ttcaaaatga gatctgagaa gaatgtgaaa
     4441 cctggggcaa tcatcacaac ggctccgaag gcaactgcag atgctgtcgc aaaaataggt
     4501 gcaatagagc tgctagctcc taaattaaag acagcaatct tattaggaat aaactgtcta
     4561 aagagccagc ttgcggtgag aatggcagct aaaagtgcta tgaaaataat gttcttctta
     4621 aaaaaatcgt ttccgaaaag gctaccggtt agggcgataa taatgagcaa caataaaggt
     4681 aagatactgg caaggaaatg tgggagattt tttgttttca cttgtatctc attgtcaaaa
     4741 gcataagtgg cataagtttc accccttgcc atactttttg ctagacaata tttcatataa
     4801 aagagcccaa aagtcacgca gccaattgag ccaacgatac tcggaatggc ggcagcagtt
     4861 agtgaggtgt ccaagtattg aataggaata acattttgaa tggcaggtgt tccgggtaaa
     4921 atcgtcatgg taaaagttgc aataccgagc caaagaggaa cttgaattaa gttccaagcc
     4981 aagtccattt ttttgaatag acttcgtgca agcggcaaga cagcaaacat gacaacaaag
     5041 aggctgattc ccccgtaagt taatatagca ctgattagaa aaatagcaac gagtacttta
     5101 taaggactat catgaccaac tttttttaaa atataatctg caatagaagt ggtttcaccg
     5161 cttgtttcca ttaatttggc taagatagaa cctaataaaa agatagcaaa ataattgaga
     5221 atataagtgg agagtgcccc cataaattgg ttgggttctt ttccaagtaa ggttgtggtt
     5281 ggatccattt gattaaacaa aatgaccaag ctggtagcaa gaggcgcagc gatgataatg
     5341 ttaacttctt tgacagacag gtaaataata acaataacac ctatcaagac ccccagagaa
     5401 ccaataattt ccatagtttt atgacctcct tattagaaaa taaagagaca gatcacaaga
     5461 tacagcctct ttatctctcc aaatttattg agcagtgtag ccaccgtcta agatacaggc
     5521 ttgacctgta acaccttttg ccttatcact ggcaaggaaa gacacatagt ctgcaatttc
     5581 ttgaacgtca atgaggcgtt tttgaggaac aagtgggtat agcacttctt caagaacatt
     5641 ttcaagggga atacctcttg ttttggaaag gtcttcaaac tggccacgaa ccagtggtgt
     5701 gtcaacatat ccaggacaaa tggcattgac cgtaatgcct gaatcagcag cttctaaggc
     5761 agttactttg gtcagaccga tcaagccgtg cttagctgaa ttgtaggcgg atttgccagc
     5821 aaaaccaatg acaccattga tagaagccat attaataata cgaccgtgtt tttgagcttt
     5881 cattgtagga aaagcacgtt taatggcaat aaatggtgct gtcaacatga ttttaatcat
     5941 gaattcaaat ttttcagttg gaaaatcttc aatcatggca acatgttgaa ggcctgcgtt
     6001 gttaaccaaa atatcaatac gaccatattt ttcaacaacc gtatcaatag cagcattgat
     6061 ggcttcttct ttggtgacat cgcacacaac actaaaagcg tcatagcctt ctttagtata
     6121 gtcagcaaca acctgatcca gtttttcttg attaatgtca gaaaaaacaa ccgttttacc
     6181 ttcttttaag aaggtttccc cgatttgttt tccaatcccg cttgcagcac cagtgataaa
     6241 tgcaacttct ttagtatgtg tcatgatact atcctcgtct ttcttttggt tagtctttag
     6301 tttgttttat caattagggt aacttcggta acgttttgca cgtcttcaaa ggtgtaatct
     6361 gggttaattt caagggcgca tagaccttca tcttgatatt caaagactcc catttcagta
     6421 atgattaggt taacaacatt ttgagcagtg agaggaaggg tgcatttgtc taaaattttt
     6481 gctttgcctt tattggtatg ctccattgca acaatgactt ttttagcacc taccaataag
     6541 tccatggccc ctcccatccc aggaaccatt tttccaggaa tcaggtaatt agcaatgcta
     6601 gcttctttat caacttgtaa ggcacctaag acggttgcgg ccacatggcc gcctcgaata
     6661 ataccaaagg aagttgagct atcgaagaaa gcccctccag gagcaatact aacaggttgc
     6721 ccaccagcat taacaatact aggatcgtag tgatcatctg ttaatccagt gagaccgaca
     6781 aaaccatttt cggactgcag ggtaatggtt acaccttcag gaatatagtt ggctactttt
     6841 gttggcaaac ctatgcctaa gttgaccagt gtattgtgtt ctaattcttg tgcaacgcgc
     6901 ttagcaatac gtgtttgaat ctcttctttt gaaagaacag tagctgatgt tcccatgtta
     6961 ggctcctttc actaagtagt taacaaaaat gccaggagta tggacaaaat taggatccat
     7021 ttgccccacg tcaacaattt ctcttgcttc aacaatggtg gttttcgcat tagcagccat
     7081 cacatgatta aagttatttt cagaacctgc gtattgcaaa ttgccatttt tatctgcctt
     7141 gttagcaaaa ataagtgcga tatcagcttt caaaggtttt tcaagaaggt agtctttgcc
     7201 gtcaacagta atgacttctt ttcctttggc aacttcggtt cctattccag taggtgttaa
     7261 gaaaccgcct agcccaaaac cgccactgcg gatgcgctca gctaatgttc cctgaggtac
     7321 aagatcaatt acagtctcgc cctcagtcat ttgtcggccc gcttcacggt tgagaccgat
     7381 atgtgatgca ataatagttg aaaactgttt attggcaacc atcttaccaa cgcctttgtc
     7441 aggaaaaccg gcatcgttac agattaaagt gaggtctttg acaccttttt ccacaagtgt
     7501 atcaattaat ttttctggtg tgccatttgt catgaagcca ccaaccataa tggtatcacc
     7561 atctttaacg tgagctaccg cttcttttat tgtgatttct ttacacattt tttctaccaa
     7621 ctttcacgag actaagtatt gtttttcacg ataacagcag ttccttgtcc tccaccaatg
     7681 caaagtgttg ctagtcctct ggttacttgg cgttttttca tttcgtggat taatgtgact
     7741 agaatgcgag cgccactggc accaatagga tgacctaaag cgatggctcc tccattaaca
     7801 ttcacgattt ctgggtttaa ctttaaatct tttaaaaccg ccaaggactg tgctgcaaat
     7861 gcttcattgg actccactaa gtccaaatca gaaattgtta atccagcttt cttaagggct
     7921 ttttgagtgg caggaattgg tccagttccc atcagttcag gtgcaacacc tgcactagca
     7981 tagctttcaa tagtgactaa aggtgttaac cctaattcaa gagctttttc ttcagtcatt
     8041 aacatcaata aggctgcacc atcgtttatt cctgaggcat tcccagcagt aacggttcct
     8101 tctttaggga ggaaagcagg acgtaattgc tgtaattttt caaggcttgt atcaactttt
     8161 gggtattcat ctgttgtaac aattaaaggg tctttgcgtc gttgaggcac agagacaggg
     8221 acaatttcgt cagcaaaacg atgattttct aatgctttag cagctttttc ttggctttct
     8281 aaagcaaatt gatcttgctc ttctcttgag atgccatatt tctgagcaac gttttcagca
     8341 gtaataccca tgtggtaatt gttaaaagca tctgttaagc catcatgaac aagagtatca
     8401 atcaattgac tattaccaag acgttgacca aagcggtgct gagttgagac ataagctgct
     8461 tgtgacatgt tttcgacacc accagcaata acgatatcag cgtcaccaag catgatgctt
     8521 tgcgcagcta attggattgc tttaagtcca gaaccgcaaa ccatatctaa tgtgaaagct
     8581 gttttatctt caggaattcc tgagtggact gcaacttgtc ttgccacatt ttgtcctagg
     8641 cctgcatgaa ggacgtttcc taggatgact tcatcaacca tttcaggctt tacttgcgtt
     8701 ttatcaagga tttttttcag tacagttgtt ccaagttcaa cagcagagac acttttaaag
     8761 gagcctccaa aagctccgat aggagttcgt aaagcttcaa cgataactat gtttttcata
     8821 aaataaacat cctttcagtt ttagaaagcg ctttccatgt ctctaagata acaagaaaac
     8881 gctaaaaata aaaatataga atttttataa gcatataatt ttttttgata gagttggcaa
     8941 tagcatttaa catctacttg tgtcataata taggtaatta taccggaaaa agtaattgat
     9001 aagacaagga gaatcctatt tggatattaa acaaattcgt tattttatcg caatcgttga
     9061 aaatcatttt aacttgagcc aggcggcgga actgttgtat gtttctcaac cgacgctgag
     9121 tatgatgatt aacgattttg aaaaaagaga aaatgttaag ctcttcaaac gaaaaagagg
     9181 tcgtattatt gggttaacct atcttggtga taattattat aaggacgctc aaaaggttct
     9241 cagtctctat gatgacatgt ttttgaaatt gcatgatcac agtaaagggt taaagggaag
     9301 tatcagtatt ggtattcctc ctcttatttt atcagttgtt ttctcagaag taatgccaaa
     9361 actgatttta gaaaatccgg ggattcaatt caatgttaag gagattggtg cttaccaact
     9421 aaaaaatgaa ctactcgtgg gaaatgttga cgtcgcagtc ttgttatccc caacaggcat
     9481 cgctgataat ttggttgaga cttatgaaat ccagagatca gaattatctg tatgcctgtc
     9541 tccacgccat cgtttagctt ccaaaaaagt tattcagtgg gaagatttaa cagatgaaca
     9601 acttgcccta tttgatccta gtttcatggt tcaccattta gtgcttgaag cttgcgagcg
     9661 tcaccaagtc agacctaata ttattttgac gtcctcttcc tgggatttta tgcttaattc
     9721 taccaagatt aatcacaacg tcttaactat ttgtcccaaa cctattacag aattatacca
     9781 attcaaggac attaagtgta ttctaatgga acgtcccatt tcttggcgtg ttgtcttaac
     9841 acgcctccgg aaaaaaagtt attctgaaat agaagcctac atcatggacg acttgctaca
     9901 gtcttttttt aagtagggtt ctcaactact tggcccaaat gttaattttt ttaaaaatga
     9961 attggaattg tgtaaacgct caatttatga taaactaaga gatggctttc tattaatgaa
    10021 atctagaata tattgaggta caaacatggt taatattatt ttattaattg tttctgccct
    10081 cattggttta atattaggtt atgcacttat ttcgattaga ctcaagtctg cgaaggaagc
    10141 tgcagagttg actcttttaa acgctgaaca agaagctgtt gatattcgtg gcaaagcaga
    10201 agtagatgct gaacacatca aaaaaacagc taaacgtgaa agtaaagcaa atcgtaaaga
    10261 attactttta gaagcaaaag aagaggcaag aaaatatcgt gaagagattg aacaagaatt
    10321 taagtctgaa agacaagagc ttaaacaact cgagacacgc ttagcggagc gctccttaac
    10381 tcttgaccgt aaagatgaaa acctatcaag taaagaaaag gtactagata gtaaagaaca
    10441 aagtctgacc gataaatcta aacacattga tgagcggcaa cttcaagtgg aaaaacttga
    10501 agaggagaaa aaagcagaac tggaaaaagt tgctgcgatg acgattgcag aagcgcgtga
    10561 agtgatttta atggagacgg aaaacaaact gacccatgaa attgcgacgc gcattcgaga
    10621 tgccgaacgt gacatcaagg accgaacagt taaaacagcc aaggacttgt tagcgcaagc
    10681 catgcaacgc cttgctggtg agtatgtgac tgaacaaact attaccagtg tccatctccc
    10741 agacgacaac atgaagggcc gaattattgg acgtgaaggc cgtaatattc gtactttaga
    10801 gagcttgact ggcattgacg ttattattga cgatactcct gaagttgtta tcttatcagg
    10861 atttgatcct attcgacgtg aaattgctcg tatgaccttg gaatctctga ttgctgatgg
    10921 tcgcatccat ccagctcgta tcgaggaatt ggttgagaaa aatcgtcttg aaatggataa
    10981 tcgtattcgt gagtacggtg aagctgcagc ctatgagatt ggtgcaccaa accttcatcc
    11041 tgatttgatt aaaatcatgg gacgcctgca attccgtacc tcgtttggtc aaaatgtcct
    11101 acgtcactct gttgaggttg gtaagttagc tggtatttta gctggtgaac tgggtgaaaa
    11161 tgttgccctt gcccgccgcg ctggtttctt gcatgatatg ggtaaagcta ttgaccgtga
    11221 ggttgaaggt agtcacgttg agattggaat ggaatttgca cgtaaataca aagaacatcc
    11281 agttgttgtc aacactattg ctagccacca cggagatgtg gagccagatt ctgtgatcgc
    11341 tgtgctagta gctgcagcag acgctctcag ttcggctcgc ccaggcgctc gtaatgagtc
    11401 aatggagaat tacatcaagc gtcttcgtga tttagaagaa atcgcgacaa gttttgatgg
    11461 ggtacaaaat agttttgctc tacaagctgg acgtgagatt cgtattatgg ttcaacctga
    11521 aaaaatttca gatgatcagg ttgtcatttt gtcgcataaa gtaagagaaa aaattgaaaa
    11581 caatctagat tacccaggaa atattaaagt aactgttatt cgtgagatga gagcggttga
    11641 ttatgccaag tagaaaaaga ggaattatcc tctttttctt tatgacttgc ctttgaaaat
    11701 gttcttgaaa aagactagcc tttttgataa actattaaaa agcaactaat aaggagaata
    11761 taatgtctga acgcggtttg ttaatcgtct tttctggccc ttcaggtgtc ggaaaaggaa
    11821 cggtaagaca agaaattttt tcaacgccag atcataaatt tgaatattcg gtttcaatga
    11881 cgactcgtcc gcaaagaccg ggcgaggttg atggggttga ttatttcttc cgcacacgtg
    11941 aagaatttga agaactcatt aaaactggtc aaatgctaga gtatgctgaa tatgttggta
    12001 attactatgg caccccttta acttatgtta atgagactct tgataaggga attgatgtct
    12061 ttttagaaat tgaagttcaa ggagctcttc aggttaagtc aaaagtacca gatggtgttt
    12121 ttgttttttt aacaccacca gatttagatg aattagaaga ccgcctggtt ggtcgtggta
    12181 cagatagtca agaagtcata gctcaacgta ttgaaagagc aaaagaagaa attgccctta
    12241 tgcgcgaata cgattatgca gttgttaatg atgaagttgc cttggcagct gagcgggtta
    12301 agcgtatcat tgagacagaa cactttcgtg tggagcgcgt gattggtcgt tatgataaaa
    12361 tgattaaaat aacaaaaaat ccctttaaag ctaaatagaa aagagtaagc attatgatgt
    12421 taaaaccttc cattgatacc ttattagata aagtaccatc taagtattca cttgtgattt
    12481 tacaagccaa acgtgcccat gaactagaag ctggtgccac tccaactcaa gaatttaaat
    12541 cagtgaagtc aacacttcag gcacttgaag aaattgaatc aggaaacgtt gtgattcacc
    12601 cagatccttc agctaaacgt gaagctgtcc gtgctaagat tgaggcagaa cgcttggcca
    12661 aagaggaaga ggaacgtaaa atcaaagaac aaattgccaa agaaaaagaa gaagaaggag
    12721 aaaaaatcta aggtttcaga aatcttagat tttttcatat ttagtacgat gtccatgaca
    12781 gaaaaggaga agcaaatgga aaaagtagct catgtgattg tagatattcc attgatgcaa
    12841 acggacaagc cattttctta tggtattcca aaagagttag tttctctcgt gcagcttggt
    12901 tctcgtgtgc atgttccttt tggaaaaggg aatcgtttgt tgcaaggctt tgttattggt
    12961 tttggccagg aagattcaag ctctcttaaa ctgattcaga ccgttttaga tcctgagcca
    13021 gtcctaaacc aagaacaatt gacccttgct gatcagcttc gaaaaacagt tttttcttat
    13081 aaaatcacgc tcttaaaagc aatgattcct aatcttctta actctaacta tgataaagtg
    13141 ctaaggccag agtcaggtct aaaaaagcct gatagggact ttctttttga aggaaaacca
    13201 tctgtcttat attctacctt agaccgcgag aaggaaaaaa tagctcttaa gggcatacaa
    13261 gctgggcata ttactgttag ttatttagcc aaagacaaga aaaacctaaa gacagaaaaa
    13321 tattatcacg tcgacttaga cgccttagcg gtacatccca tctctagtcg tgccaaaaag
    13381 cgtcaattat taaaagacta tctgctaacc catacgaagg aagctaaact tgcaacgctt
    13441 tatcaagcct tttctagaga tgtggtggct tactttgtga caaatcactt aattcgtatt
    13501 gacgaacgtc aaattgatcg cagtgagccc tattttgacc agatcaaacc atcttctttt
    13561 ttaaccttaa atgaacagca ggcatcagct gttactgaaa ttgttgagca aattgggaaa
    13621 ccgagtaaac cttttttgat tgaaggtatt acaggttcgg gtaagacaga agtttatctt
    13681 catatcatcg aagcggtttt aaaacaggat aagacagcca ttgttttagt accagaaatt
    13741 tcgttgactc ctcagatgac ctctcgtttt atttcccgtt tcggaaagca agtggctatt
    13801 atgcattcag gcttatcaga tggtgaaaag tttgatgagt ggcgcaagat aaaaacagga
    13861 caggctaagg ttgtggttgg tgcccgctct gctattttct ctccactaga gcggattggt
    13921 gcaattatta ttgatgagga gcatgaatca acctataaac aagaaagcaa ccctagatac
    13981 catgccagag aggttgccct gctcagagct aaacaccatc aagcagttgt ggttatgggc
    14041 tctgctacgc caagtattga aagccgagca agggctagta agggtgtcta tcattttatt
    14101 caattaacga aaagagcaaa ccctcttgct aagattccag aagtaacgat tgttgatttt
    14161 cgtgattata ttggacagca agcagttagt aattttaccc catatttgat tgataagatt
    14221 aaagaacgtt tggttaaaaa agaacaagtt gtcttaatgt taaatagacg tggttattct
    14281 agttttgtga tgtgtcggga ctgtggttat gtggacaaat gtcccaactg tgatatctct
    14341 ttaacccttc acatggatac taaaactatg aattgccatt actgtgggtt tcaaaaacct
    14401 atcccaatta cttgtcctga gtgtcatagc aacagtatcc gctattatgg cactggaact
    14461 caaaaagctt ttgatgaatt gcaaggggtt atcccagaag ctaggatctt gagaatggat
    14521 gttgacacca cacgaaaaaa aagaagtcac aaaaccatac tagatagttt tggtcgccag
    14581 gaagcagata ttttgttagg gacgcaaatg attgccaaag gactggattt tccaaatgtg
    14641 actttagttg gggtactaaa tgcagatacc tccttaaatt tgccagattt tcgagcgtca
    14701 gaaaaaacgt ttcaattgtt aacacaggtt gctggtcggg caggccgtgc ccataagcca
    14761 ggggaagtct tgattcaaac ttataatccc gatcactatg ctattcaatt agctaaacaa
    14821 caagattttg aagcctttta ccgttatgaa atggcgattc gccgtcagat ggcttatccc
    14881 ccttactact ttacagtggg tattacgctt tcgcatcgtt tagaagcatc ggttgtaaaa
    14941 aaagcttatc aagttacaga actgctaaag agtcacttat cggataatat taaaatatta
    15001 ggaccgactc ctaagccaat tgctagaaca cacaatttat accactatca aattttgtta
    15061 aaataccgtt ttgaagataa cctagaagag acgctcaatc gtattcttga ttggagtcaa
    15121 gaagcagata atcgtcattt aaaactaatt attgattgtg aaccgcaaca atttttataa
    15181 ggtaaatcac taaatctagg atgatcagtt atgatataat aaaaatacaa agaggaaagt
    15241 catgataaaa ttattattta tgggaactcc ccaattttca gcaactgtat tgaagggctt
    15301 gctagataat cctgcctatg agattttagg agtggttact cagcctgatc gagctgttgg
    15361 acgcaaaaaa gatattaagg taacacctgt aaagcaatta gctcttgagc atggaatatc
    15421 aatctatcag ccggaaaaat tatcaggctc tcaagaactc attgagatta tggaacttgg
    15481 tgcagatggt attattacag cagcatttgg ccaattttta ccaacaatat tacttgattc
    15541 agtttcattt gctattaatg tacatgcatc attgttacct aaatatcgag gtggagcacc
    15601 tatccattat gctattatga atggagataa agaggctggc gttactatta tggaaatgat
    15661 aaaagaaatg gatgcaggtg acatggtcgc taaagcaagc actccaattc ttgaaaccga
    15721 taatgtaggt actctttttg agaaattagc catcattggt cgagacttac ttttggatag
    15781 tttgccagct tatctttctg gagaattaaa acctattcct caagatcaca gccaagctac
    15841 tttttcccct aatatctctc cagaacaaga aaaattagat tggacaatgt ctgatcaaga
    15901 agtgttcaat catattcgag gtatgaaccc ttggccggta gcgcatacgt ttttagaggg
    15961 gcaacgttta aaaatttacg aagctcaatt agctgaagga gaaggattac ctggacaagt
    16021 tattgtcaaa accaaaaaat ctttggtaat agctgctggt caaggagccc tgtcattgct
    16081 ggttgtgcaa cctgcgggga aacctaaaat ggctattgca gatttcttaa atggccttgg
    16141 tagaaaactt gaagtgggtg atttcattgg cagataattg gaaaataagt attcgaggaa
    16201 aagcacttct aaccttagaa catatttttg atcagggggc ttacacaaac atagcgctta
    16261 atcagcagct aagcaataaa gctttatctg ctaaggacag agctctatta actgagattg
    16321 tatatggtac cgtttcacgt aaaattagct tggaatggta tttagcccat tatgtcaaag
    16381 accgagataa attggacaaa tgggtttatt atctccttat gctcagtctt tatcaactga
    16441 tttatttaga taagttacca gcgcatgcca ttgttaacga tgcggttggt attgccaaaa
    16501 atcgtggcaa taaaaaaggt gcagagaagt ttgtcaatgc tattttacgt caattcacca
    16561 gccatcctct cccagatatg gaaacaatta agcgtcgaaa taagtattat tcggtcaagt
    16621 attcactgcc tgtttggttg gttaaaaagt tagaagacca atttggttct gacagatcag
    16681 tggccatcat ggaaagttta tttgtgcgta gtaaggctag tattcgcgtg acagaccctt
    16741 tgaagctaga ggaagctgct gaggctttag atgcagaacg ttccctttta tcagcaactg
    16801 gtttaaccaa agcgtcaggt cattttgcag ccagtgatta tttcacaaat ggggacatca
    16861 ctattcaaga tgaaagcagt cagcttgtag ctcctacttt aaacattgac ggtgacgaca
    16921 taattcttga tgcatgtagc gctccagggg gcaagacctc tcatatagca tcttacttaa
    16981 aaacaggtaa agttattgcc ttagatctct atgatcataa actagagttg gtgaaggaaa
    17041 atgctaaccg cttaggagtt gctgataaca ttgagacaag aaaactagat gccagagagg
    17101 ttcatcgtca ttttgaaaag gatagctttg ataagatttt ggtggatgct ccttgctcag
    17161 gaattggttt gattcgtcga aaacccgata tcaaatataa taaagaaagt caagatttca
    17221 atgctctaca ggctatccag ctagagatat tgtctagtgt ttgtcaaacc ttgcgtaaag
    17281 gtggtataat aacatacagt acttgcacta tttttgatga ggagaatcgt caagtcatcg
    17341 aagcattttt acaaagtcat cctaattttg aacaagtaaa actgaaccat acacaagcag
    17401 atattgtcaa ggatggttat cttattatca caccagaaca ataccagact gatggcttct
    17461 ttattggaca agtcagacga gtcttgtagt attttaggag gaaactgact gagtcagaaa
    17521 aaacgtatga agatttcatt aaaaacagac attgggcaaa aacgctcaaa caatcaagat
    17581 tttatcaata agtttgataa taaaaaaggc atcactttag tgattttagc agacggtatg
    17641 ggtggccaca gagctggaaa tattgccagt gagatgacag tcacagacct gggaagagaa
    17701 tgggtaaaaa cagacttcac agagttaagt cagattagag attggttatt tgaaactatc
    17761 cagtcagaaa atcaacggat ttatgatcta ggccaatcag aagactttaa aggaatggga
    17821 acgactgttg aagcagtagc tttagttgaa tctagcgcca tttatgctca tattggtgat
    17881 tcacggattg gtcttgtcca tgatgggcat tataccttat tgaccagtga tcattcttta
    17941 gtgaatgagt tggttaaggc aggacagatc acagaagaag aagctgctag ccatccacaa
    18001 agaaatatta tcactcaatc tattggtcaa gcttccccag tagagcctga tttaggagtg
    18061 agggttttgg agccagggga ttatcttgtc attaacagtg atggtctaac caatatgatt
    18121 tcaaatgatg aaattgtgac tatcttgggc agtaaggtta gtcttgatga aaaaaaccaa
    18181 gaaatgattg acttagctaa cctaagagga ggcctagata atattacaat cgctttggtt
    18241 cacaacgaaa gtgaggacgt cgaatgattc agattggcaa attatttgct ggtcgttatc
    18301 gcattctgaa atctattggc cgcggtggta tggcggatgt ttatttagca aatgatttga
    18361 tcttggataa tgaagacgtt gcaatcaagg tcttgcgtac caattatcaa acagatcagg
    18421 tagcagttgc gcgtttccaa cgagaagcgc gggccatggc tgaattgaac catcccaata
    18481 ttgttgccat ccgggatata ggtgaagaag acggacagca atttttagta atggaatatg
    18541 tggatggtgc tgacctaaag agatacattc aaaatcatgc tccattatct aataatgaag
    18601 tggttagaat tatggaagaa gtcctttctg ctatgacttt agcccaccaa aaaggaattg
    18661 tacacagaga tttaaaacct caaaatatcc tactaactaa ggagggtgtt gtcaaagtaa
    18721 ctgatttcgg catcgcagta gcctttgcag aaacaagctt gacacaaact aattcgatgt
    18781 taggcagtgt tcattacttg tctccagaac aggctcgcgg ctccaaagcg acgattcaaa
    18841 gtgatattta tgcgatgggg attatgctct ttgagatgtt gacaggccat atcccttatg
    18901 atggcgatag tgctgttacg attgccttgc aacattttca aaagcctctt ccatctatta
    18961 tcgaggagaa ccacaatgtg ccacaagctt tggagaatgt tgttattcga gcaacagcca
    19021 agaaattaag tgatcgttac gggtcaacct ttgaaatgag tcgtgactta atgacggcgc
    19081 ttagttataa tcgtagtcgg gagcgtaaga ttgtctttga ggatgttgaa agtaccaaac
    19141 ccctccccaa agtggcctca ggtcccaccg cttctgtaaa attgtctccc cctaccccaa
    19201 cagtgttaac acaggaaagt cgattggatc aaactaatca aacagatgct ttacagcccc
    19261 ccaccaaaaa gaaaaaaagt ggtcgttttt taggtacttt attcaaaatt cttttttctt
    19321 tctttattgt aggtgtagca ctctttactt atcttatact aactaaacca acttctgtga
    19381 aagttcctaa tgtagcaggc actagtctta aagttgccaa acaagaactg tatgatgttg
    19441 ggctaaaagt gggtaaaatc aggcaaattg agagtgatac ggttgctgag ggaaatgtag
    19501 ttagaacaga tcctaaagca ggaacagcta agaggcaagg ctcaagcatt acgctttatg
    19561 tgtcaattgg aaacaaaggt tttgacatgg aaaactacaa aggactagat tatcaagaag
    19621 ctatgaatag tttgatagaa acttatggtg ttccaaaatc aaaaatcaaa attgagcgca
    19681 ttgtaactaa tgaatatcct gaaaatacag tcatcagtca atcgccaagt gcgggtgata
    19741 aatttaatcc aaacggaaag tctaaaatta cgctcagtgt tgctgttagt gatacgatca
    19801 ctatgcctat ggtaacagaa tatagttatg cagatgcagt caatacctta acagctttag
    19861 gtatagatgc atctagaata aaagcttatg tgccaagctc tagctcagca acgggctttg
    19921 tgccaattca ttctcctagt tctaaagcta ttgtcagtgg tcaatctcct tactatggaa
    19981 cgtctttgag tctgtctgat aaaggagaga ttagtcttta cctttatcca gaagaaacac
    20041 actcttctag tagctcatcg agttcaacgt caagttcaaa cagttcttca acaaatgata
    20101 gtactgcacc aggtagcaac actgaattaa gcccatcaga aactactgct caaacacctt
    20161 aacaaagaat agttagtctc atgttttact agcatgccac taactattct ttttaactac
    20221 tcgtcatata aagatgcaat tttccaggaa ttctgataaa atacaaagaa ataaggggaa
    20281 actaatgaaa aaatttcaat tctttttact tatcgagtgt atactgctag ctatgggtat
    20341 catgacaatc ttggataatg atttatcaag ttttatccta attctagttc ttatcttatt
    20401 agcattacgc ttttataacc aagatagccg caataatttt ttactaactg tcagtttatt
    20461 gtttcttttt ttaattttca tgcttaatcc ctacattatc atggctgttc ttttggggat
    20521 agtttacatt ttcattaacc atttttcaca agtcaaaaag aagaatcgct ttgccttgat
    20581 tcgtttcaag gaagaaaaga ttgaagtgaa caataccaag catcaatgga ttggtactgc
    20641 taattatgaa agtgattatt attgttttga tgacattaac atcattcgaa tctcaggaaa
    20701 tgacacggtt gacttaacca atgttattgt gacaggaatg gataatatca ttgtgatacg
    20761 taaaattttt ggaaatacga ctatattggt acctattgat gtcactgtta ctttagatgt
    20821 tagttccata tacggaagtg ttgatttttt tagatgtcag caatatgatt tgcgcaacga
    20881 atctattaag ttaaaagaaa cggataacca atcccttaaa aaagtcaaaa ttatcgttac
    20941 aactattgcc ggaaatgttg aggtggatag gcgatgaaaa aacgttacta tgctcttgtt
    21001 tggctctact caaccattac cattttatct attgtttttg tggtcatgga taatttagga
    21061 atcactttta actaccttcg caatcattta tggcaggtcg aacgtctagg gttttcgatt
    21121 ttattgttga ttgtttctgt gactttatta ttgttgttat tgtggattat tatggatgat
    21181 aacagtaagc gtaacatcaa ccaaaatcta aaatatattc tcaataatcg acgactctat
    21241 ctagatgaga catcagaaat caatactaat ttaagtcgac tttctaaaaa aatgtctcac
    21301 ctgactgcta acatgcaaaa gaaagaaagt gcttatattc ttgatagcca agaagttgta
    21361 aaacaagagc gcaaacgaat tgcaagagat ttacatgaca cagtcagtca agagctattc
    21421 gcttcgtcat tgattttatc aggaatttca atgagtttgg aacaactgga caaaacacaa
    21481 ttacaaacac agttaacaac ggttgaagca atgttgcaaa atgctcaaaa tgatctacgc
    21541 attctccttt tgcatcttag acctaccgag ctagctaatc ggactttatc tgagggcctt
    21601 catatgattc ttaaggaatt aacagataaa agtgatattg aagtcattta taaggaaacc
    21661 attgctcagc ttcctaaaac aatggaagat aatcttttta gaattgccca agaattcatt
    21721 agcaacacgt taaaacatgc taaagctagt cgaattgaag tttatctcaa tcaaacctca
    21781 acagaattac aattgaagat gattgatgat ggtgtaggat ttgatatgga tcaggtaagg
    21841 gatttgagtt atggtctgaa gaatattgaa gaccgtgtca atgatttagc aggaaaccta
    21901 catttaatta gtcaaaaagg caaaggagtt tccatggata ttagactgcc gatagtgaag
    21961 ggagatgacg atgagtaaga taaaagtgat attggtcgat gatcatgaaa tggtccgcat
    22021 gggactcaag agttttttga atttacaagc tgatattgat gtcgttggtg aggcctctaa
    22081 tggacgtgaa ggggttgatt tggcattggc tttgaagcca gatgttttgg ttatggatct
    22141 agtgatgcca gagttaggcg gtgttgaggc aactttagaa gttctaaaaa aatggaaaga
    22201 ggctaaggta cttgtgttaa cttcctatct agataatgaa aagatatacc ctgtcattga
    22261 tgcaggagcg aagggttata tgttaaaaac atcgagtgcg gctgaaattt taaatgccat
    22321 tcgcaaggtt tcaaagggag agttagctat tgaaacagaa gttgacaaaa aaattaaggc
    22381 gcatgatcaa caccctgact tgcatgagga actaacagcg cgtgagtatg atattttaca
    22441 ccttttagct aaagggtatg ataatcagac catcgctgat gaactcttta tttccttaaa
    22501 aaccgtcaaa acacatgtgt ccaatattct agccaagtta gaagttgatg accgaaccca
    22561 agcggttgtc tatgcttttc gacatcattt agtcccccaa gacgataatt aatgctataa
    22621 tatagggtat agcaaattgg aggaaagtat ggacgcaaaa ctaaaataca aagctaaaaa
    22681 aatcaaaatg gtgttctttg atattgatga taccttgagg gtcaaagata cgggttatat
    22741 gcctgagtcg attcaaagag tatttaaggc tttgaaggca aaaggcatct tggttggtat
    22801 tgcttcaggt cgcgctcggt acggtgttcc acaagaagtt caagatttac atgctgacta
    22861 ttgtgtcaaa ttaaatggag cttatgtcaa agacgatgct aaaactatta ttttccaagc
    22921 tccgattcca gcagatgttg ttgtggccta taaaaaatgg gcggatgata tgggcatttt
    22981 ttatggaatg gcgggtcgtc atgaagcggt gttgtccgct cgaaatgaca tgattagcaa
    23041 tgctattgat aatgtctacg ctcaactaga ggtctgtccg gattataatg agtatcatga
    23101 tgtttaccag atgtggactt tcgaggacaa gggagatggc ttgcagttgc ctgctgaatt
    23161 agcagagcac cttcgtttgg tcagatggca cgataactca tcagatgttg tcttgaaagg
    23221 tacgtcaaaa gcgctaggtg tttcaaaagt agttgaccat ttaggattaa aacctgaaaa
    23281 cattttggtt tttggagatg aactcaatga cctagagtta tttgactatg ctggtatcag
    23341 tattgcaatg ggagtatcac atccattact gcaagaaaaa gcagatttta tcacaaaaaa
    23401 agttaaagaa gatggcattt tatatgcctt ggaggaatta ggattgattg acaaagaatt
    23461 acagtttcca caattggatt tgccaaacca taaaggacca aaagcaacca ttaaaacaaa
    23521 ccatggcgat atgaccctgg tcttgttccc agatcatgct cctaaaacag tagccaattt
    23581 cttaggtttg gctaaagaag ggtactatga tgggattatc ttccaccgta ttattcctga
    23641 atttatgatt caagggggcg atccaacagg aacaggaatg ggtggacaat ccatctatgg
    23701 tgaaagtttt gaagatgaat tttcggatga actctataat ctccgtgggg ccttgtcgat
    23761 ggctaatgcg ggaccaaata ctaacggcag tcagttcttt attgttcaaa atagtaagat
    23821 tccttatgcc aaaaaagaac tagaacgtgg tggttggccg gctccaattg cagcttctta
    23881 cgctgcaatt ggtggaactc ctcaccttga tcgtcgtcat acggtctttg gtcagcttgt
    23941 ggacgaaact tcattccaag ttttggattt gattgctggc gttgaaactg gcgcacagga
    24001 taaacctaaa gaagacgtta tcattgaaac aatcgaggta tttgactaat gaaaattggc
    24061 gacaaactgc atggcaccat cactgggatt aaaccatacg gagcctttgt tgcccttgaa
    24121 aatgggacaa caggtctcat tcatatttca gaaataaaaa cgggatttat tgacgatatt
    24181 gatcaattat tggctatagg caaccaggtt ttagtacaag taattgatat tgatgaatat
    24241 agtaaaaagc caagtttatc tatgcggacc ttagcagaag aaaaacagca ttttttccat
    24301 cgtcatcgtt attccaacag tcggcacaag attggcttta ggccacttga agagcagtta
    24361 cctcagtgga ttgaagaaag cctgcagttt ttaaaaaaga acactaaaaa gagtctggaa
    24421 ccttagtagt tccaaaccct tttttatttc gtcttaatta ctggcacatc atagagttca
    24481 gtcgataaat agcgttcgcc attatctggt aaaatagtta acacatgttt gccttttcct
    24541 aattgtttag cgacttcaat agcggcgtaa agagcagctc cagaagaaat cccaaccagg
    24601 aagccttcct tagctcctgt tagtcgagca gtttctaaag catctttcga tttaacacgg
    24661 ataatttggt cataggcttt ggtatctaac gtgtttggga taaatccagc tgatatacct
    24721 tgaattttat gtggtccagg ctcttgacca gataagacag cagattcttc agcttcaaca
    24781 gcatagataa cagtttcagg gctagctttt ttcaagacat gtgaaacacc agaaagtgtt
    24841 cctccagtac caacaccaga tacgaatgca tctaaagaaa tctccttaaa agcttccaaa
    24901 atttcttgag ctgttgtttt ttcatggatg cttggattgg cagggttatt aaattgcata
    24961 ggcatccaag cacctagttc tattgctaaa gtttcagctt ttgcaatagc ccctttcata
    25021 ccttctgctc caggtgttaa gacaagctct gcaccataag cctgaatgat ttgtcgtctt
    25081 tccaagctca tagtttcggg cataacaata atgactcgat accctttagc agcacctacc
    25141 catgcaagac caatacctgt attaccgctt gttggttcga taataacgtc accaggactt
    25201 atcagacctt cagcttcagc agcttcaatc atcgataaag caatacgatc tttaacagaa
    25261 gatcctgggt taaaagcttc taattttaca taaacgtcag cagcttcgtt tggaattaaa
    25321 cagttaagtt tgataatagg tgtttgacct actaattctg ttatagtttt gtaaatttta
    25381 gtcatagttt agcctctttt cttgatacaa aataaatacg ctgtgtattt aagtataagg
    25441 agagatatta ttaaagtaaa atacagattt actatcactc tgataataaa gccatatcac
    25501 ttttgttgat gattaatcac aggggaattc aataactttg gatccgattt tctcacagct
    25561 tatctgacca tgataataat ttgttaaagt atcaatgaga ttttccacac gttcagtatc
    25621 acaataaagt gttgtcttta tcgtgtctga aaacttagtt tctgtttcgg ttaaagccaa
    25681 ttgatctagc aagttagagt atagttggta ttggggataa gaaagtgtga tttctagtcc
    25741 tatctgctgt ttaacttcaa tgatgccaaa tcgtttaata gctgttgcgg tcatgttaga
    25801 gtatgctctg attagcccac ctgttcctaa tttaatacca ccaaagtaac gcgttaccac
    25861 aactactaca tttgttaaac actgcttttc taacacagaa aggataggta ttccagcagt
    25921 tcctgaaggt tcaccatcat cacttgagcg cttgatctga ctgttgttac cgataatcat
    25981 cgcaaagcag gaatgattag ctttgtaatg ttcttttttg atagcattaa caaaattctt
    26041 accatcttct tcagtgctta ctcgtttgat atgacaaatg aaacgagatt ttttaatact
    26101 ttcttcgaaa aaaccactgg ctttgattgt cttaaaatgt tccataaaaa aattgtacaa
    26161 gaaaagataa aaattagcaa aaaaaagcga ataataaagt atggagtcta ttgaaaatag
    26221 ttatggacga ttgttgctag aaagccagtt accagattca gcaaagcagc ttgctcaacc
    26281 tttaaaaagt gtagtaatat cgagaggaaa aatgatttgt caacgttgtc actatcagct
    26341 tgatgaagaa gcacgattgc caaatggagc ctattattgc cgattttgtc ttgtctttgg
    26401 acgaaatcaa tcagataaat tgctctatgc tatcccacct atgcattttc caaaaggtaa
    26461 ttatctggtt tggagcggtc aactaactgc ttatcaagag atgatttccc aacaattatt
    26521 aatcaatatg caaaatcaaa aaacaacatt agttcatgct gtaactggtg caggaaaaac
    26581 agaaatgatt tatgctgcga ttgaggcggt aattaacaca ggtggctggg tttgcattgc
    26641 cagcccaaga gtagatgttt gtgtagaggt ggctactcgt ttatcccaag ccttctcctg
    26701 cagcatctgt ttaatgcacg cagagtctct tccttatcaa agagctccta ttatagtagc
    26761 cacaacgcat cagctgctaa aatttcacaa ggcttttgat ctcttaatta ttgatgaagt
    26821 agacgcattc ccttttgtaa ataatattca acttcattat gctgcaagtc aggctttaaa
    26881 agaaggtggc gcaaagattt tattaactgc aacttcaact agaactttag aaagaaaggt
    26941 aaacaaggga gaagtagtca agctgacatt ggcgagacgc tttaataata ggccattagt
    27001 tattccgaaa tttataagaa gttttaatct atttaaaatg atacatcggc aaaaacttcc
    27061 tcctaaaatt ttaaaatact taaaaaaaca aagaaaaaca ggctatcccc ttttaatttt
    27121 tctacccact attataatgg cagaatctgt tacagcaatc ttaaaagagt tattgcccgc
    27181 cgaacaaata gcttgtgttt ctagtcaatc acaaaacagg aaagaagata tcacagcttt
    27241 tcgtcaaggg aaaaaaacga ttttaattac aacaacaatt ttagaaaggg gtgtgacatt
    27301 tccccaaatt gatgtttttg ttcttgggtc tcatcatcgt gtctatagtt cccaaagtct
    27361 tgttcaaatt gctggacgag ttggaagatc tatagacaga ccagacggaa cgttatattt
    27421 ctttcatgaa ggtatcagta aagcaatgct actagcaaga aaagagatta aagaaatgaa
    27481 ttataaaggg tattcgcatg atctgtctac tatgtcaaca aattagtcaa acaccaataa
    27541 gtattacaga aatcatcttt ttaaggcgta tctcttcacc gatttgtcaa caatgtcaaa
    27601 aaagctttca aaagatagga aaaagtgttt gtgcgacatg ttgtgcaaac tcagatataa
    27661 tagcttgtcg agattgtcta aaatgggaaa acaaaggata caatgtaaat catagaagct
    27721 tatattgtta taatgctgct atgaaagcat acttcagtca atataagttt caaggagact
    27781 atttattaag aaaagttttt gcagtagaac ttgccgatgt tattaccaag tactataaag
    27841 gctatatccc agtcccggtt cctgtaagtc ccggttgttt tcgagaaaga caatttaatc
    27901 aagtgagcgc tattcttgag gcagctaatg ttagctacct ttctcttttt gaaaagctag
    27961 ataatactca ccaatcttcc agaacaaaaa aagagagatt attagtagaa aaatcttatc
    28021 gactactaaa agtatcaaac attcctgata aaatccttat agtagatgat atttatacta
    28081 ctggtagtac aattatcgct cttagaaaac aattggctaa agtagcaaat agtgacatta
    28141 aaagtttgtc aattgcacgt taaaacttgc aaattttcat gaaagcagta taatagtatt
    28201 aaagataaag taagcgcttc aagcgcgaga aagaggtatc ctatgattaa gttcagtatt
    28261 cgtggagaaa atatcgaagt gacagaagct attcgcgatt atgtagaatc aaagcttact
    28321 aaaatcgaaa aatattttgc taaagaccaa gaaatagatg cacgtgttaa tttaaaagtt
    28381 tatcgggaga gatcttcaaa agttgaggta acgatcccat tagattcagt aacccttcgt
    28441 gctgaagatg tttcacagga tatgtacggc tctattgatt tagttgttga taagatcgaa
    28501 cgccaaatta gaaaaaataa aaccaaaatt gctaaaaaac atcgcgaaaa ggttccaact
    28561 ggtcaagtct ttacaactga gtttgaagct gaagaagtcg atgagatacc agaagtacaa
    28621 gttgtacgta ctaaaaatgt gacattaaaa ccaatggatg ttgaagaagc gcgtttacag
    28681 atggaactat tgggacatga tttctttatc tacactgatt ctgaagatgg tgcaacaaac
    28741 attttatacc gtcgcgaaga tggaaaccta ggattaatag aagctaaata gttcacaact
    28801 aaaacctaca gggatgatta tacgtatttt aaaagtggga aaaagctatt catgtttcga
    28861 ggtgacatga tgaaaatgag attaagtgat agatacgaca gtcattatat aacaaaagta
    28921 tctattacag ttcctcttta agtaaatctt acgtaaaatt agtttgaaaa aaagattagt
    28981 cccaattctg ggctagaaat tctcaaaaaa tatgaaaaag cgcgttttca gaatataaag
    29041 cgcgtttttt tctttttcca tttaacaata aaaaac
//
LOCUS       DATUYR010000020        27634 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.020, whole genome shotgun sequence.
ACCESSION   DATUYR010000020 DATUYR010000000
VERSION     DATUYR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27634
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.020"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            219..971
                     /locus_tag="VCI33_000791"
     CDS             219..971
                     /locus_tag="VCI33_000791"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEP2831726.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            1027..2100
                     /locus_tag="VCI33_000792"
     CDS             1027..2100
                     /locus_tag="VCI33_000792"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEP2831727.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKRIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(2535..2840)
                     /locus_tag="VCI33_000793"
     CDS             complement(2535..2840)
                     /locus_tag="VCI33_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP2831728.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPRDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKAHHLEAKTDCKLLQVLLD"
     gene            complement(2842..3180)
                     /locus_tag="VCI33_000794"
     CDS             complement(2842..3180)
                     /locus_tag="VCI33_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP2831729.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAAIPHALYAVEAFQ
                     MLLVVVKPEA"
     gene            complement(3233..3988)
                     /locus_tag="VCI33_000795"
     CDS             complement(3233..3988)
                     /locus_tag="VCI33_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEP2831730.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVTVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAKAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            complement(4240..5118)
                     /locus_tag="VCI33_000796"
     CDS             complement(4240..5118)
                     /locus_tag="VCI33_000796"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP2831731.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEIRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            complement(5256..8762)
                     /locus_tag="VCI33_000797"
     CDS             complement(5256..8762)
                     /locus_tag="VCI33_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEP2831732.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNVKQEIDMTPDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQDPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENASYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYANGKDYDKLHEEHSDWLLYGSET
                     ASAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNVTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPAHLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKVSQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKVSTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWYLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLATSQKRLTYCYQYDTVQAIGA
                     ISLQNHTDTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLVVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            complement(8692..10176)
                     /locus_tag="VCI33_000798"
     CDS             complement(8692..10176)
                     /locus_tag="VCI33_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2831733.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFDVVSTARSGQEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTNHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDWLDAQQAESIRQETYHDSLLTLWLTDELSEKEFHQLSQD
                     LPEHALTGFTVLYVECSEWIEEMEAYFKQEEQPCYLKKEEDKKIYLAILLGRAQQAKA
                     FTSNLQKQFSENIQQIILGETVDDWENVYESYNQVRQSLFYNSQMSQPTDKGNSKLEM
                     PESRLQFFAFNKALMIGDEPTILAKLEAIFHEMKALNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHNSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            complement(10176..11900)
                     /locus_tag="VCI33_000799"
     CDS             complement(10176..11900)
                     /locus_tag="VCI33_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2831734.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQLQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDV"
     gene            complement(11890..12495)
                     /locus_tag="VCI33_000800"
     CDS             complement(11890..12495)
                     /locus_tag="VCI33_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEP2831735.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLMTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            complement(12801..14246)
                     /locus_tag="VCI33_000801"
     CDS             complement(12801..14246)
                     /locus_tag="VCI33_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP2831736.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            complement(14327..15253)
                     /locus_tag="VCI33_000802"
     CDS             complement(14327..15253)
                     /locus_tag="VCI33_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP2831737.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            complement(15263..16213)
                     /locus_tag="VCI33_000803"
     CDS             complement(15263..16213)
                     /locus_tag="VCI33_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2831738.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIVFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLVGSG
                     ADPIKWYSNPTWWPLILLFMSGWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            16409..17287
                     /locus_tag="VCI33_000804"
     CDS             16409..17287
                     /locus_tag="VCI33_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP2831739.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAISSPGAINKEKGVIEGASAIPYIHHFKIQEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVLGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALAEQGDHLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            complement(17898..19340)
                     /locus_tag="VCI33_000805"
     CDS             complement(17898..19340)
                     /locus_tag="VCI33_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP2831740.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWTRLIDDFEQVTINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRQGPAELSDGR
                     IGTILNLTPAYPASQSEADMAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDAYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIRF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(19365..21059)
                     /locus_tag="VCI33_000806"
     CDS             complement(19365..21059)
                     /locus_tag="VCI33_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEP2831741.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDMPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAELTVKHFFNRQLDKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFAMRGIIEGFYGTPWTREERLDCLRFIGRKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQSDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSNSWLETLTDYTGDAELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVDELVERVNTLRKLDKPAFQQAIVPWFERVAKDQDFWQAI
                     LEQGPHLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            complement(21110..22150)
                     /locus_tag="VCI33_000807"
     CDS             complement(21110..22150)
                     /locus_tag="VCI33_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2831742.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAEKQVTSPEANKDLLLILPFTSDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LYIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            22283..23569
                     /locus_tag="VCI33_000808"
     CDS             22283..23569
                     /locus_tag="VCI33_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEP2831743.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSITP"
     gene            23584..26289
                     /locus_tag="VCI33_000809"
     CDS             23584..26289
                     /locus_tag="VCI33_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEP2831744.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLQVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDNSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLSDLANDLPDNL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLTQNHPHDSICGCSVDSVHREMINRFEKAYEVGHYLAKEAAQQIAGAV
                     NTSDFPTDSQPFLLFNTSGHSKTSVTELTLTWKKYYFGQRFPKEVYQEAQEYLAGLSQ
                     SFQVIDVSGNTISEADILDTSIAFDYDLPKRTFREPYFAIKVRLRLPITLPAMSWKAL
                     ALQLGNETAPSATVPLYDDSNQCLENEFLKVMIQTDGRLTITDKQSGVSYQDLLRFED
                     CGDIGNEYISRQPTHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPISADKLLQA
                     EMEAVIDITERQARRSQEKAELTLTTLIRMEKNNPRLQFTTRFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPDATFWKNPSNPQHQECFVSLFDGENGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPILVEQTSHHKGSLRAEHSYVTGADDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(26390..>27634)
                     /gene="rocA"
                     /locus_tag="VCI33_000810"
     CDS             complement(26390..>27634)
                     /gene="rocA"
                     /locus_tag="VCI33_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEP2831745.1"
                     /translation="NIFYLSLSMVLFQVVFWAFFPDHFILDVVMLAQFLFFALIALYY
                     GKSIKAKFLMFYAFFPLVSISLVKRFIVFFVMPLFGMPYSVVKHNTLLIYSITCFSIF
                     LIYRCIQVFHFDFSTWRQYFQSHRASKLLVFTNSSMALYYLCVQGIDVMSPSLSGLAT
                     TTARSIIVLFYFILFLTLLIHLERYVKQNSIEAIVQQKEYRELINYSQHLGLLYQDIQ
                     ELRQLLTTVSSRLKIGIEQNDISIVRLTYEGILNAEKNNAKDDRLDLTCLDKLQVEAI
                     RHIVLAKLIEAKNKKLKVEVSIPNCIATFFLEVVDFTKLLSFLLDNAIEMSLETKQPC
                     LSIAFLDQNHKLVIVIQSSTKQGQNDSQSVFATPALKKRDDWQFDLRNVTTILNRYDY
                     LTISSQIHDGILTQLIEIAKPD"
BASE COUNT         8269 a         6031 c         5318 g         8016 t
ORIGIN      
        1 taagatttgg ggcagtattt ttttatagct agcaagcctt ttttaataaa aatgactaag
       61 aacatagaca atctagggtt tacttgctat ttcatcttgt ttatggtatc atttcacagg
      121 ctaagggtgc ttcatggata acaacgagat tttgtattcc taaataagac gttatcccat
      181 agagcacgcc atacagaatt agtaaaggaa tcaaacgaat gattgacatt atgtctgact
      241 ttcaaaataa gacttgttct aagaataatt ttattgttgg accttgttcc attgaatctt
      301 atgatcacat tagattagca gcttcaagtg ccaaaaaact tggttacaac tattttagag
      361 gaggcgccta taaaccaaga acatctgctg cttcttttca aggccttggc ctgcaaggaa
      421 ttcgttactt gcatgaggtt tgtcaggaat ttggcttact atcggtcagt gaaatcatgt
      481 ccgagcgcca attagaagaa gcctatgact atctcgatgt cattcaggtt ggtgcccgca
      541 acatgcaaaa ttttgaattt ttaaaaacct tgtcacacat cgataagcct attttgttta
      601 aacgaggctt gatggctact atcgaggaat atttgggcgc cctttcctac ttacaagaca
      661 ctggaaaatc taacattatc ttatgcgaac gtggcgttcg tggctacgat gtggagacta
      721 gaaatatgct tgatattatg gcagtcccta ttattcaaca aaagacagat ttaccgatta
      781 ttgtggacgt gtctcattcg acaggcagac gagatttact cttacctgct gctaagatag
      841 cgaaggctgt cggtgccaat ggcatcatga tggaggttca tcctgatccc gaccatgcgc
      901 tgtcagatgc tgcccagcaa attgattata agcaattaga acagttgggg caggaacttt
      961 ggcaagatta agctctaaat atcactgcgt tatctctgtt acctagtact gatgaaagga
     1021 gtgcttatgc cacaaacact tcatgtccat tctcgggtca aggattatga tatcctgttt
     1081 accgaccatg ttttaaaaac cttagcagat tgccttggtg agcgcaagca gcgtaaactc
     1141 ttgtttatca ccgatcaaac ggtctaccac ttgtatcaga ctctctttga agaatttgct
     1201 cagcaataca acgcttttgt ccatgtctgc cctccgggag gtcaatccaa atccttggaa
     1261 cgtgtcagtg ctatctacga ccagctcatc gctgaaaatt tttccaaaaa agatatgatt
     1321 gttactattg gtggtggtgt tgttggggat ctgggtggtt ttgtcgctgc aacctattac
     1381 cgtggtattc cctacattca aatcccaacg actttactta gtcaggtgga tagcagtatc
     1441 gggggcaaag ttggagtcca ttttaaaggt ctaaccaata tgattggcag catttaccct
     1501 ccagaagcta tcattatttc aaccaccttt ttggagacct taccgcaacg ggaattctcc
     1561 tgcggcatca gtgagatgct caaaatcggc ttcattcatg atagaccgct ttttcagcag
     1621 ttacgtgatt ttcaaaaaga aaccgataag cagggacttg aacgtctgat ttaccaatcc
     1681 atttccaata aaaaacggat tgttgaacaa gatgaatttg aaaatggttt gcgcatgtct
     1741 ttaaattttg ggcatacctt gggccacgcg attgaatcgc tctgtcacca tgatttttac
     1801 catcacggtg aagccattgc tatcggtatg gtggttgatg ctaaactggc tgtttccaaa
     1861 ggactcttgc caaaagagga cttggatagc ctccttcaag tttttgaacg ttaccagtta
     1921 ccaacaacac ttgaaagagc tgatgtctct gcgacatccc tctttgacgt ctttaaaaca
     1981 gataagaaga attctgagca acatattatc tttatattac ccacagaaac cggttttacc
     2041 accctagcca ttaacaaaga cgatcaccaa tttgtcgaga agctcgacag tttattataa
     2101 atagtgactc cactctagct agttaagctt gctaagagaa taacggtatc ctggaactcc
     2161 taaaacatgt tactacaaga caagtcttga taacaatata acttttagct aagaataact
     2221 aacagcaaca tcaaaaagag gatttatgga actataggag aacggtatca ctcgtaaaaa
     2281 gatctttctt ttgcctgtcg ttcaattatt tcgtctaaaa agccttaatg agaaacctat
     2341 aaggagatag gaaactcatt aaggtttttt agtgaattct aaaataaacc aagctaaagg
     2401 gcttaatctg gagatgagaa ggccaagctt taaaattcgt attgtcatcg ctttgaatac
     2461 gtaaaaagag aagatgagcg tcatcttctc ttttgctttt ccataggcca cccaatacag
     2521 ttgacaataa gcccttaatc taaaagcact tgtaatagtt tacaatcggt tttagcttct
     2581 aaatgatggg ctttttgggc aggtatggct atgagacttt cttgagttag gacctgtttt
     2641 tgatcctcta aatcgaaaat gagctgacct tctaaaacaa gtattacttt atctcttgga
     2701 ctagtctctt gactaatgct ttctccttgg gccatggcat ataaaacaat agattgagat
     2761 aatcccagta gacggctcaa ggatcggcta gccacctgat gatcatgata ttgcaccagt
     2821 tgagagagat tttcaatcat gttaagcctc cggttttact actactaaaa gcatttgaaa
     2881 ggcttctact gcatataagg catgaggaat agcagctggc atcacaatgg tttgaccttc
     2941 agcaacccgg taggtctctt gatcaatggt gatttctgct aagccactta agatagtaac
     3001 catggcatct cctggtgatg agtgtcgccc aatttcttgg cccttatcta aggaaaaaac
     3061 tgttattcct aggtcttgcc tttggactag tgttctactt aacatttgat cttgctcaat
     3121 cattacttct tgcgtcaaat caagaacctt cgcgtgctca atattattga tataagacat
     3181 cttcgaactc ctctatggtc tgtttagttg tatggacagg ttggtctaag gtttatttta
     3241 cactttttac agcaatatag cagagatttt tgcctgttct ggtaaaaaaa cgacgcatct
     3301 ttaaaaactg ttgtctgttc tctttcttaa ggcctcgtct cataatgttc agggtgttca
     3361 taagaccttc atccttaatc attccacgag gtgtcatcaa agacattttc ccatggtgat
     3421 aagtcacttg agaaaaaccg tgttcttgat ataaggcttg ccaacctgtt agtgacaagg
     3481 gagcaacatt gacatgaatc gtttgacgta actcgtcaag tacttgtctt gtttttggat
     3541 cttcataagc tacgtcatgt gtcaaaagtc gcccgcctgg ttttaacaca cgccaatatt
     3601 cattgagtgc cttctccttg gctttggcgt tcaacatggt taacatggct tcattaatca
     3661 caatatcaaa atggttatct ggaaatggta gtgataaagc attggcttgg gatactgtaa
     3721 tgtgacgggt taacccctca acagtaaccc tttcttgtgc ttctttgaca accttggggt
     3781 ttaagtcaac ggcatcaatc tgacaaccga aggttttggc taactctatt gctgtcgtgc
     3841 agcgattgca ggcaacctct aaaacgtgac aatcctgaga gaattgtcct tgttcaatca
     3901 accagtttgt cgcctcaaca ccgccaggtc ttaagcgttt ttttcctaga cgtgctaaaa
     3961 agtcatgccc aacttccttt gttaccattg ctattctcct ttattcattg aacttttcct
     4021 tttataagag cttcttttct aaaaaaacgg taggttatct ggcagacctc aaaggaacta
     4081 tgacttgtat cacgttaggg ataaggctct tcaaataagc tttttactct taaaaacagc
     4141 tctcgcaaaa agaattttag cagcccatga tgactgctcc atctcttatt aatatatcat
     4201 aattaagctt acttaaaaag ttctttaaaa aaggttgagt tactcatcac ctaaaagagg
     4261 tttaatagcg tcaactggca tgtcttgacc agtgattaat ttaaaagctt ctgctccttg
     4321 ataaagcacc atccctagcc cattaatgac tttttgggcg ccatgttgac gggcaaaggc
     4381 tagtaacttg gtttctgctg ggctgtacac gatgtcaaag accaccaagt ctgggcgaat
     4441 gagctcgggg tcggtaatca aacttagatt ttctaaaggc ttcattccta cacttgtagc
     4501 atcaataaat agatgagatt ccctgatggc ctccttaaag gcagtctgat cttccaagtc
     4561 ttgaagggtt actttagtcc ttgttagctg gttcagtttt gtcacaagct tttgaacgct
     4621 acttagacgt gtagcttgtc ggttaaacaa acgaatctct ttggcgccgt caaaagcgag
     4681 ctggacagcg atagctttcc cagccccacc aactcctgct agggtaatga ttttgttctt
     4741 taccgacact ccttcgtcag ccaaggctct gagcgctcct atgccgtctg taatatgccc
     4801 cacgagatgt cctttaccat ccttattgac aaccgtattg actgcaccga ccaactcagc
     4861 agctggggac aagtcatcca ataagggtaa aatagcttct ttatttggca tagacacatt
     4921 ggagccacga attcctaagg cacgaatccc ttgcacggca tctgctaatt gctctgtccc
     4981 tacttcaaaa gcaaggtagg cataatccag ccccagttta gcataggctt cattgtgcat
     5041 tttgggagac aaactatgac gaataggagt agcaagcaaa gagaccagca gggtgtggcc
     5101 tgataaacgt tctgacattt agaaatctca ctttccattt caaccttgta aaggcgcctt
     5161 tcacgtaata aaggacgcct atttgttttt tattatacct tattttacaa aatttatcag
     5221 acctggtgcc aaagaagtct tcttttactt atgcttcatg ctaaatccca aaggccaatt
     5281 tgaatactgt cttcagttag gtcaggtcct tcaatgactt caagggtcac tactagatac
     5341 aaaacagtca aaatatcatc gaagaaaata acctcttcta actggctcaa gtggctatca
     5401 gctagcatca aggtttcttg cttaatagag ctctgactac cataactgat tgccacagaa
     5461 acactaagag atgtgtctgt gtgattttgc aagctaatgg ctcctatggc ttgcaccgta
     5521 tcatattggt agcaataagt tagccttttt tgacttgtgg ccagggattg cttggcgact
     5581 aaactgagat cctggtgccg tcttttgcta gcttgtgcta ggttaagcct tgcctcaacc
     5641 ggtattgatt gcccaaaaca agaaacatag cccgtgacaa gaataggctt ggcaaagatt
     5701 ttctctttta ggtaccatgt cattggaaaa gcttttttca caaaagaacc ttgggcatct
     5761 ttgacttgga gccactgagg caagaagata tcgtcgcctt ttttaaaatc aaatgcctga
     5821 gaacaaacct gcaagggcag agataaatag tgctggtaac tggtgataag aatgtgaggt
     5881 gtgctaacct tacctgcttt ttgctgggtg tctttgttct ccaaaacaga aaggaattgt
     5941 gtggtcttta gacaagattg accaatttct tgttgacttg tcagtcggct ctttttaaac
     6001 tgatatttct ctaaaggtag tgtcactaag tgttctttgg gggcacggca ggctacttgt
     6061 gtctgaaaaa cactgctaag ttgctgcgag acttttgccg tgaccttaat gtgtcccgcc
     6121 tctccagtga gggctaaaat ggctaataat tttcccccat atgcctggcg tagaggctcc
     6181 tgataagggg tgtggtcagt cggattccca ttatcaagag ccaacaaatg ggctggtcct
     6241 tctacttgaa cctttagcca atcttgagca tggctgacca attcacctcg gctatccaag
     6301 agtgataaat ccagataaag caattcccaa ggagcgtcaa taggtgcttc aaaggctttc
     6361 caagatagct ttgccactga accataggtt ctaacctgag aacgtccact ggtttttgga
     6421 atgcttttac cagaaatgtc gtaggccact gcccttagca accctttttg atagggcact
     6481 cgccaagtca ggtaaagatt ctcgtgagga ttcttagcag cgtcagctcc ttggtaaagc
     6541 tgataagtgt gtcccgttgg cgtgctataa gtatgaaatg ccttccttcc ataatctgtt
     6601 acgttgccct gctcatcttc aaacatcagt tgaacactgg cagcattgct ataaaccaca
     6661 acctccacca atccctgctc atcgaaacaa agctggtcct tctgccagac tggtaacagg
     6721 tgcaaggtgg tctgcccctg cgcccactga ctttggtaaa aataatagct atcttttgga
     6781 aagcctgccg tgtctaaaat gccaaagtag gcatttttag gagaaggcca aaggcctaca
     6841 acaccgctgt cgatcttgtt ccagggtgta ggttctccta aataatcaaa gcctgtccaa
     6901 acgcattcgc cagcaacaaa atcacgggta atggtatcat accaagcctg gctagcgaat
     6961 gcaccccaat cgaccttagc atgatcataa gaggttaggt gataaccgga gtcaagaact
     7021 ttttttgtct ctttataata agccctgctg gtaatggctg aggctgtttc agaaccatac
     7081 aacagccaat cagagtgctc ctcatgaagc ttatcatagt cttttccatt agcatagttt
     7141 aacccaatga gtccctgggg atggtctaat tgactaagaa gagttgccat ttgcgacact
     7201 tcctcatgcc agcaaaagtc agcttctttt aatttattgt ccccaaaggt aatgggacgt
     7261 gaggtgtcaa tagcagcaat ccattggcac atttgtcttg ttagctctgg gtaatgactg
     7321 acatcggctg aaaaaccttc catcaattca ttcccaatcg accacatcaa cactgacggg
     7381 tcattcttgg cagaccagac catggcctca atactatact gggcccagct cgtttcaggt
     7441 gacctcactc tttgaagata gcttgcattt tcagttccga tggtctgatg gaaatagttt
     7501 gaaaagtcat taacatttcc atttttggca taggtccagg tgtcaaaggc ttcttcaatc
     7561 acaaaaaagc cgagacgatt ggctagttgc ctaagtttgg gactacttgg gttatgggtg
     7621 ctacgaatgg tattagcccc catgtctttc aacaggacta attgtctggc tagggcatct
     7681 tcgtaagcac aagctcctag gcctccttgg tcatgatgca aacaaactcc ctttaatttg
     7741 acagctctgc cattcacaaa aagcccctga ttggccataa aagtcagctg acgaaaacca
     7801 gtttcaaggc aaaagcaatc aatgacctga ccttgatagt aaagagtgag ttctaaatcg
     7861 tagaggtgag gattatctgg tgaccacaat tggagttctt ccaaagacag tgtaagacca
     7921 tactgtcttt ggaaagccaa atcagctaga ggcacttctg tttcttgata aaggagtttc
     7981 ttgtcttttg agcaatgtga ttgttcccat aaacagaggc tcaattggta gtggcaagtc
     8041 tgaatagact gattgagggc aaagcgtaaa tcaactttct gttgcccttt ctcctgcaca
     8101 gcagtatcag ccaaggtcat tgctacctgg tctgccacaa aatgaagctg gggaagaaca
     8161 gacaaactca cttcacgata aagaccactg cctgaatacc aacgagaact gggaaccttg
     8221 ttttcaaccc ggacagccag aagattttca gctccaaaag tcacaacatc agagatatca
     8281 taagtaaatt cctgatagcc atttaagtgt ttgccaatga attgcccgtt aacataaatt
     8341 tccgtttcca tataggaacc ctcaaaagta atggcaacat gacaaccagc taaaacctca
     8401 tctaacacga gataatgccg ataccaaccg acaccaccta atttataagc actttcagct
     8461 tcaccatttc ttgtgtaagg ctgcgtaaga ctgaaatcat gtggtaaatg aatcacctgc
     8521 cagttcgaat catcaaaaga agggtcctga gcacacgcca catctgccat aagaaaacgc
     8581 cagccttgat taaagggctg ctttcttcct tcttgatcaa tatagtggta ttggtagcca
     8641 gtcactgtct gctccttgct atctggtgtc atatcaatct cctgtttgac gttactggtc
     8701 gcccttgatg gcttggtaat tgtccttgta acgatctctg aactcttttg gcgataaacc
     8761 attcaacttt ttaaacatct tgataaaata atgattggta ttgtaacctg tttcataagc
     8821 aatttcattg atggattggt tggtgtaaag caataactgt tgcgcttttt gaatcctaac
     8881 atgatttaag tactgggtaa aactacgctc ggtttcattt ttaaagcatt gccctaaata
     8941 aaccccattg acatgtaacc tatcagcgat agctttaagg gttaattctt gatggtattc
     9001 cttgcgaatg caatcaatgg tttcactaac taaatctgaa taacgttttt caggggaatt
     9061 gttctgattg gtgacatcca acactttttt tagttccctc aaaatgcgat caatggattg
     9121 gctgttatga atagttttta ccatggacag ataagtcatt ttatctaaaa tgggaaattg
     9181 acggtaaatg tcagagaaca acaagaacga cacatgcttc acatcctctg gtgagaagtt
     9241 caaggccttc atttcatgaa aaatggcttc taatttggcc aagatggttg gctcatcacc
     9301 aatcatcaat gctttgttaa aggcaaaaaa ttgcaaccga gattctggca tctcaagctt
     9361 actgttgcct ttgtcagttg gttgtgacat ttggctattg taaaaaagac tctggcgcac
     9421 ctgattgtag ctctcataaa cattttccca gtcatcaacc gtttctccta ggatgatttg
     9481 ttggatgttc tctgaaaatt gcttttgtag attactggta aaggctttgg cttgctgggc
     9541 tctcccaagt aaaatcgcca agtaaatctt cttgtcttct tcttttttga ggtaacaggg
     9601 ttgttcttct tgcttaaaat aagcctccat ctcttctatc cactcggaac actccacata
     9661 cagcacggta aatcctgtta gggcatgctc ggggagatcc tggctaagtt gatgaaattc
     9721 tttttcacta agttcatctg ttaaccacag tgtcagaagg ctgtcatgat aggtttcttg
     9781 ccggattgat tcagcctgct gggcatccaa ccagtcttta aattgtttca ttttagcctg
     9841 caattctgcc ttatccacag gttttagcaa ataccccttg gtttctaatt ccatagcctt
     9901 ttgcacatag gagaattctt gataaccaga taaaatcaag gtttgtagct gaggatgatt
     9961 agttttagct gcttctatta aatccagtcc tgtcattcca ggcatggtaa catctgaaat
    10021 catgacatcc actgggtact gagtcaaata ggctaaggct tcctggcctg atcttgctgt
    10081 tgaaacgacg tcaaaaccaa gagcctgcca atcaataatc atggttaatc cttgtagaat
    10141 catgtattca tcgtcaacta ataatacctt atacattaaa catcctttct aatcctaatt
    10201 tgaatgataa agccaccatt tggctcctta gccatggacc aagaaaccct gtctcgaaaa
    10261 tgatggaaaa gacggaggta aacattttgc aaaccaatag agctattgcc tgttgtttgg
    10321 tgctcttgaa gccttttttc catatctgct aagcgctctt gactaatacc acgtccatta
    10381 tcaagcacct gaatcaagag atgatccgtc tcatctaatg ccttgatgct tagtgcattg
    10441 tcatggcgac tataatcaat cccatgcaca aaataatttt caacgagagg ttgaatgaca
    10501 aatttaggaa tggctaaatc agcaatactc tcatcaattt ttacatgata agcgaagcta
    10561 tctggatacc gcatttggta gaggtaaatg tacttttcac aaaaagccag ttcttctttt
    10621 aaggtggtca tcttatcttg gctaatattg ttgcgaagca aactcgcaaa ggcataaatg
    10681 acatctgcta attcttcttg ttgacaacta agggcgtaca tccgaatata ctctaacgtg
    10741 ttgtataaaa aatgagggtt gatttgagac tgcagagctc tcatttgggc atctcgttgc
    10801 tctacctcta acacataaac ttcgtcaata taagctttaa tgctggccaa catctgatta
    10861 atggcttctg agatatgata cagttccaat tccatgtggc tgttgtcgat ggttaaagac
    10921 aaatcgcctg ctgcaaccat ttcaacagta tccgtaattt cagaaacctg ctggatataa
    10981 tttcggaaaa ccctaaagag caactgcaat aacagccaag ctaagagtag agaaacaagg
    11041 ccatacacaa aaactgtcct gacagcttcc gctaataaat aggattttct gacagccaca
    11101 taagctaaga ggtctcccga gcgattgtac tgcatccgat aagccttgct gagattacta
    11161 agatcagctg cccgatctgc cttgatagcc tttttaacct cagcctctaa atgaggaaat
    11221 ctccgacctc cttgatggaa gagtttgcgg ccatagtaat caaaagcaaa agtagctaaa
    11281 tagtgatcct gctgtgtctc taccaaagtc ggtggtttgt caaaggtcag gtaaagacga
    11341 cctgtcacat ctcctgattc tggatttcga atggagcgaa ctaaaacatt tccggttact
    11401 ttttcaggct tgctataaag caaacgaccc gtcttaatga ccctatcggc gtacagataa
    11461 gcactactgt cctctagcag tagggtcata gaagtcacat cagggtacaa gtcaaaaaga
    11521 cgcctcacct cttcagaaaa taggaccgtg tcttttcctt tttcccagtc ttcaaaaacg
    11581 tttgaaaaat attggtttgg ggttaacgtc atatattgat agaggttatg gtaatcttct
    11641 gaacggccaa ccaactggtc taaaacccgt agggtaatat cgttttgtaa ctgcaatcta
    11701 cctagagtct cctccagtcg catctgcgcc tcagcatcca cccttttata agtactacta
    11761 atagaataac tgctcaagcc aatagctgct aaagtgaaaa aacttacaat tagcaggcta
    11821 tataatttca tcagtcgatt aatcaaaaaa ccctgcttaa agtggttagt cacatacctt
    11881 cccaatgttt taacactcat caatcactcc atctactagt cgacgattag ccttagtgac
    11941 aaagtccaag caaaagacta gagctccgaa ggttaaaaag agataaaggc ctttgtattg
    12001 ccaagtcatt aaggtaattc ccagcaaaac actcagcatg agtaaaaagg atttgaaact
    12061 taaaaagacg ctgataaagg ctaatttcat attattccaa aggttaatct catagctcac
    12121 tgcataaaaa accatgtaaa gataggtaat ggttagccaa accatggcta ttacaatcaa
    12181 gaaagacacg gtaaaccaaa acaaggatgg aaactgtact gcccaatgca aattaaaggt
    12241 taaaaaaagg aaagtcaggg caaaaagacc aaatcttaga ttagcttctt tgaaataaga
    12301 tttccaaagt tgaacagccc ttttcaaaga atagtggttt tcttcttgag gtgtttttaa
    12361 aaatagggtg acaatggttt gccaggctgg ccccacccca aacacaatca gtccagctaa
    12421 ggacaagaga tgaaaaatga gggtcaattg gatcattttc caaattaaaa agcaggtttt
    12481 ttcaatgcca ttcatatgat agtcctcttt ttcttgttaa cacttatagc ggtttcaagg
    12541 accagcagct acgagactag ttttagcacc cccagtaaga tgctggtatt agttttactc
    12601 tgattaatag tttagactct tctaatatag cacactttat aggcttttgg aaaccgcttt
    12661 actaagagta gtaacattat ttacattaat ttttctccct ttataacttc cacttatttg
    12721 atgatccgtt ttttccactc atcagaagaa aagagctgag atttcccagc cctcaactta
    12781 taatcaagaa aatgaatgcc ttacttgtct ttagctacaa agtcgtccaa ttgtttttga
    12841 acttcttttt gcaccttgtc ccaaccagca cctttgaggt ctgcaagaag ttttggaagg
    12901 gcttcgtcag gatccactgt accagtattg atacttgctt tatatcggtt catgacattt
    12961 gaaatatttg ataattccgt tttgataact tttgtgtcaa cagtgaaacc aaggattgga
    13021 gattctttag catccttgat tgattggtca cgtttagcaa tcatgtcgtc agtaacagat
    13081 tcttgagtgt aaaggatttt attgttacca gtattccaag cacccatgtg catttttggt
    13141 tgatagccat cgagcagttt gattttcttg tcgccaattt tttcccaagc tttgccttcg
    13201 acaccataaa caagcccatt taacaattcg ggatcgctat taagaagaga aaggacttca
    13261 actgcttttt ctttgttttt agatacgctt gataccacaa agtttgccat ttgtgcttgt
    13321 gatgtggttt ttagcggttt agtcaatgga cgagacacga tgtcttttcc tgcagcattg
    13381 gtcaagatag tatcgccata gtccatagga ccttgggttt cttcacgcat aaaccaagtg
    13441 tttccttcaa ggggataacc ttctgtattg gtcgctgcat ctgttggaat caaaccttct
    13501 ttataccatt tgtgcatcaa gcggagattg tttttaaagg actcatcttc atactgatta
    13561 atgatggttg gcttgccttc atcaattttc acagcaaagg gttgggtttt ggttaatggg
    13621 tagtcatagt cacctgacat actaaagact tgaccaatag caaaagctgc tgtatttggt
    13681 tctttttcgt ggaattgttt caagacattt tcagcatctg cataggactt aatgtttgaa
    13741 atgtcaaggc catatttgtc cactagttct ttattgaaag aaagcatctg ttgggcataa
    13801 acgttggcat ctactgggaa ggcatagagt ttaccgtcaa tagtatttcc tttaatatag
    13861 gctgggtcta agtttttata cgttttctta gcgtattttg gcattaacgt tgtcaaatca
    13921 gcaaaagcac ctttttgtgc attgaccaca taattattgg caaaagcaat gtcatagttt
    13981 tcaccagagg caatgatggt actcatttta tcatcccagt ccccccaacc gatgtattga
    14041 aggtcaaccg ttgcacctgt tttttctttg atgcgcttgt tagcaattgt catcaattca
    14101 tcgaagttat caggtttgtc accaacttgg tacatcaata atttgacatc actatccttt
    14161 gatgctgact tgctttcgca ggctgctaaa ctagacgctg caagcacagt tccagtcaca
    14221 cacacgattt tttgccactt tttcataagt gaagcctcct aaaaaatgtt ttcttttgtt
    14281 actaatgatt agataaaatc tggtcttgtg accagttcct taagtcctat tccttcaccc
    14341 caccaatggt caaaccaccg acaaagtact tttggaagaa tgggtaagtc aaggcaatcg
    14401 gcaaggttgc aataaccacg atagccatac gaactgattc ttttggcagc gatgctaagc
    14461 tatctgccat ttgtgcactc atgccagcat tttgtgccaa ggcctgtagg ttgctctgaa
    14521 ttttcatcag taagtactgc attgggtaga ggttttcact ttgcacataa agcaaggcgt
    14581 taaaccaatc attccaataa gttaaggcag aaaataaact gatggtagca atccctggaa
    14641 cagccaaagg gagaacaatt ttcataaaaa tcatccattc actagcccca tccatacgag
    14701 ccgactcaat aatgctatct ggaactgtct ttttaaagaa cgttctcatg accaagatgc
    14761 caaatgggcc cagagccatt ggtaaaatca aagcccaaac cgtatccttc aaatgaagga
    14821 gattactgac caccaagtaa ttagcaacca tccctggtgc aaagagcatg gtgataagtg
    14881 cataaacagt aaaaaaacgt ctgtatggga aataaggtct tgaaatcgca taagcatagg
    14941 ttgatgtcat ggtcgtgtta ataaaggttc ccaccactgt aataaacacg gatatcccaa
    15001 atgacctcat gattctatgg gacatggctc cttgaaaaat atactggtaa gccttagttg
    15061 accagacatc tggaaaaaaa ctatacccat gattaatcaa gtaactttca tcagtgaagg
    15121 aaataataat cacgaagata aaaggaatga tacaagagat ggcaaacaga ccaatcagaa
    15181 tattaaagat agcattggtt tttcgatcaa aggttctaac attaaccttt tcgacttttt
    15241 tctttttctc catgttttcc tcctaaaaca aggctgcatc aggatccatg cgacgaacaa
    15301 tcgcattagt gactaacaag atgctggtcc caactactga ctgataaagg ctagctgccg
    15361 atgccatccc aatatctcct gtagcagtca aggcattgta gacataggta tctaacacac
    15421 tggtcacgtc ataaagtggg cctgaattct tgggaataac gtagaaaagt ccgaaatcag
    15481 atttaaaaat attaccaata ttaatgatca ataagataga catcattggc aataattgtg
    15541 gaatggtgat gttcctaatt tgttgccatt tgcttgcacc atccaccata gcagcttcat
    15601 aataagtatc agaaatccct ttgactgaag catagtagat aatgctattg tagcctagcc
    15661 ctttccagcc actcataaag agtaaaatca agggccacca agtgggattg gaataccact
    15721 taattggatc tgctccactg ccaaccaaga gctgattaat gaaaccttta tctgtattta
    15781 aaaaggcaga aacaaagtat tcaataacca cccatgataa gaaataaggc aacaaggaca
    15841 tggtgtgata gaccttaacc gttcgcttat ttcgtaactc actcatgata atggcaaaaa
    15901 caatcgcaaa gaaaacatta aaaagtaaga aaatcacatt gtaagcgatg gtatttctgg
    15961 taatcaacca agcatctttg gaagcaaaga gatatttgaa attagccaaa ccaacccaag
    16021 ggctctcctt caaactttcc ataaagcccc ctgctgaata atgaaaatct ttaaaagcaa
    16081 ccacattggc taaaactgga atataaaaga aaaagatgaa ccagatgaaa cctggtaata
    16141 ccattaataa taaggcccgg tatttaatca catttttcca aaaggatgtc tttttgttgg
    16201 tttgtcttgt catttcccaa cctccctttg tgttaatcac gttatagtta cagtatatca
    16261 agcgctttca ttattttaaa gcaacaaatt ataggtatga taacacaaag tatagaatca
    16321 cttagaaagc cttttcattg ttgcttaaat ctgtcatact aagatgagac atgatcgtct
    16381 attctactat aataaaggag aacacctcat gagcttgtta tgtattgata taggaggcac
    16441 ttcccttaaa tttgccctct gtcacaatgg ccaattaagc cagcaaagca gctttcccac
    16501 tccctctagt ctggaaaagt tttaccaatt actcgaccaa gaagttgctc gttactctgc
    16561 ttaccatttc tcaggtattg ccatcagttc acctggggca atcaataaag aaaagggggt
    16621 tatcgaaggc gccagcgcta ttccctacat tcaccatttt aaaatacaag aggccttaga
    16681 agaacggcta cattacccta tttctatcga aaatgatgcc aattgtgccg ccttagctga
    16741 agctacctta ggggcaggaa aaggcgctag tagcctagcc atgctcgttc ttggtactgg
    16801 agttggagga agcttggtca ttgatggcaa gatttaccat ggtgcccact tatttggggg
    16861 cgaatttggt ttcatgatca tgaacgaccg ctaccaaacc tttagtcagt tagggacagt
    16921 ggtaaatatg gccaagcgct atagtgcaat cgtcaataac ggaaaagatt atactggcaa
    16981 agctgtcctt gccctcgcag aacaaggaga ccacctagca ctcaaagaaa ggcaggtctt
    17041 tctccaaagt ttagccatcg gtatttttaa cattcaacac gcctttgatc ctcaactgat
    17101 tttaatcggg ggtggtgttt cccaagctga ctttctactg tcagcgattg aagctgaact
    17161 agacaaactc taccaagccg tcgaaatctc cgaccttaga ccccaattag ctatttgtca
    17221 ctttaaaaac gaagccaatc ttcttggagc tgccattgat ttttaccaag aacacaagga
    17281 ggcctaacca tggaccgcta aatccctctt atcccctatt gtccctaatc gcctctgaca
    17341 tctacaaaga catcctaaaa ggattgctga ttagctacaa cgatactcgc tttctgccta
    17401 aatgactctc tgctgatgaa cacagcctga tgcctggtcc ctttgttgat gtcattgttg
    17461 ctgacgcttc tgttaaaggc attggtcatc agttaatact cgagctatta gaagctatgc
    17521 tagttatcgc caataagcaa agcctcactt aataactcac tcatatagtc cccttatcgt
    17581 ggacagtgac ataacaaaca gtcactagaa accatgaggg gcttttatct ggtccaaaaa
    17641 tatgaaagaa aatcctaaaa taccaagatg aaaaacaagc atccttagcc aagatactct
    17701 aaagaattga agagccaaat tacccaagat ggatagatga tgccaaacca gacagaacgc
    17761 ttatatctag ttcggcaaat accaacacct tccaaagaaa tacaagagcc tcgatcctct
    17821 taacatgagg aaaaaggccc ttgtttaatc attggtaaca tcattattca cttgtctcaa
    17881 aggagacgtt cacacgttca atcactgagg tgagaagcag agcctccaaa ctcctccatt
    17941 acttctaatg aaatcaagcg gttatgagta gctacttttt taaaccaata agctgaagct
    18001 ttaggtcgtc gcacttgggt atggatatta ttttccacaa ggccatagcg gtttttatag
    18061 gcattgagcc aagaccagcc atcaattggc gtccatacat ggtatccaaa acaattcgag
    18121 cctgcttcta ttcctttgtg gaggtaggtc aaatgttctt tcaaaaatcg gatgcgataa
    18181 tcatcttgaa tctgaccagt ttcatcacgg taacggtctt ctcctgaaat ccctacacca
    18241 ttttcagata agaaccaagg aatattgtcg taatggtctc gcatcttgat ggcaatatca
    18301 tagacagcct caggatagat ttcccacccc ttatccacgt tcatgcggcg gccaggcatc
    18361 aggtaggcgt cataatacca ttcaggactc caagatgggc taatcactgg aatgacatcc
    18421 ggagccttca cgcgcttagg gtggtaaaaa ttcaatccca aataatccac tctattctca
    18481 gcaataagag ccaattcttc tggcgttgac tgccacaaaa ccccatcttt tttcaatacc
    18541 gcaaccaatt cttctgggaa tttcccatga acagcagctt ccataaacaa gtcattgttc
    18601 caaagttctg caaaatgcgc agcagccatg tccgcttctg actgactagc tgggtaggct
    18661 ggcgtcagat tgagaatggt tccaatacgc ccatcactaa gttctgctgg accttgcctg
    18721 taagcttgaa taaccttggc tgtggctagg gctaggttat aggcgacctg gactgctttt
    18781 ttcccatcca cgatagcagg gtaatgaaat tgcattaaat aagagccttc cacaacgacc
    18841 ataggttcat tgtggacaaa ccaatcctta acccgatcac caaactgctc aaaacaaacc
    18901 ttgctaaagg cgacaaataa gtccacgaca tgctttgatt cccagccacc ataggcttga
    18961 tagagggcaa tgggcaggtc aaaatgatgc aaattaatga caggacgaat accattggcc
    19021 aagcaagcat caatcactcg attataataa gcaaggccat caggattaat cgtcacttgc
    19081 tcaaagtcat caatcaaacg cgtccactga atggaggtgc ggtaggaatt gtgacctagt
    19141 gaagctaaca aggccaaatc agattcaatc tgatgatagg catccgaagc cgtatcaggt
    19201 cccacgtaat cgtaaaacaa gtcaggctcc tcttcatacc aataatcaaa gagattgcgg
    19261 tgctgtttag caaatcgccc ctcactttga gggcctgaag tggctcctcc ccaccaaaac
    19321 tcttttggaa atgctaacat atcattcctt tctagctcgt atgactaagc ataatcttca
    19381 ggtcttgctt gggtaacttg accttgatta gctgttagtt tatcttgctg ttgatagtag
    19441 atgcgatagt agcgacttgg aatgtcactt ccaatacggt gtttatcttc ttgtaagtca
    19501 gcatagagtg tttccaaatg aggcccctgc tctaaaatgg cctgccaaaa atcttggtct
    19561 ttggccacac gttcaaacca aggaacaata gcttgttgaa acgctggctt atctagcttt
    19621 ctcaacgtat tgacacgttc taccaattca tctacccaag ctgataacgt tgatacatcc
    19681 ttagccagga gagcttcttc cacttcaaaa ggaaggtccc tgtgcagatg acgattgcca
    19741 ttatgccaag caaatgcctg taaagctaag gctaattcag catctcctgt atagtctgtt
    19801 aaggtttcta gccaagagtt actaggctga taacggttag catcccacag ataatggctc
    19861 atgtctgtca aggtcagctt agataattcc caactaatca tagggttaga aacaatcccc
    19921 ttgacctgat agtctgattg gcataaaaat ggtgagcgat tagcaaaagg ggttaaaaag
    19981 agacgttctg gatctttttg ataatcatta acaggaatat tgtcccaaat aatgataggc
    20041 cgctgatata ctgctgccat ggtttcgata tctgcttgcg agatttgact cgctagcgtg
    20101 cttgggcctg tccagaaaaa ggcgacttct ttaggaatcc tttctgacag ctcttgcaaa
    20161 tagatagaat catggtggtt gtcgtattct gtcggacaga tcaccaattc tggcgccgca
    20221 tgttgttggt ttaaaaagtg atgaacctca gttgccaagt gggcctgggc ataggcagtc
    20281 tttttgaatc gtctctcaac agcatctaca atttggtagt caatatcatc cagcaaaagc
    20341 ccaaaatgac aaacacctaa cgctagaagt tgttgcaatt tttgatagag caactgatag
    20401 tcagcttcct tggtataatc aaaatcaagt cccggactaa tcatatacca aaaatcaatc
    20461 ccttcttcct tggctactgc aagtagctct ttgaaataag tcacccaatc ctcaggataa
    20521 agatcgcgcc acagttttcg ctggtaatca tcatctttcg gagcatacat gtaagtattc
    20581 atccttttac gtccaatgaa acgcaggcaa tctaaccgct cttctcgtgt ccacggtgtt
    20641 ccgtaaaagc cttcaattat cccccgcata gcaaaagaag gactatgctt gacagataca
    20701 atcggtaaat aaagtttatc ccctttgaaa gtcatcactt taagcaaagc ttcttgggcg
    20761 taacgaaaac ctctaaggtt ttggctttca ataataattt ccttatcttc tgaaaccttc
    20821 agggtaaagg catccttatc cagctgacgg ttaaagaaat gtttaacggt caactctgct
    20881 tggccttctt caatccactg aaaatacttt gacgttgcca attctgccag tagggctttt
    20941 tgattttcaa aacggaaaaa acggccaata aagcggcatg tcaacggcat gtcaaagaca
    21001 agatagtctc ctcttgtcat gacttcttga taatccttta aggcgtgata aatggtcatt
    21061 tctggtctcc tttacctgag ctaaccagct catgctagta acgtcttttt tagtgactac
    21121 tttctctgat aaatacttga acaggtaact ggcaagacac cactgcttgg tctggctcat
    21181 gaataatctg caacaagact ttagctgctt cttgccccat ttggtaaaaa tcttgagctg
    21241 ctgtgcttaa tttgggactc aacaaacggg aggcttgact attatcaaag cctacaatag
    21301 cgacttgatc aggaatatat aagcctacct gtgtgatttt ttgaatcagc tgaatagcca
    21361 accaatcgtt ttccacgaca agcccccttt tagtggctgt cgtctccgat aaggttttca
    21421 ccagacggtc caaataagca tctgttgttt cagtctctgt cttaggaaaa taatgggatt
    21481 ttagagacga ttctgccatt gccgctaagt aacccaaata acggtctcta acggatgata
    21541 cttctccaaa agagcctgtt gccacaaaac taatctggtc acagccttgc tcaatcaagt
    21601 gctgagtcaa ttgacaggca ccgcctttat tatccgctac aacagacgga aactgaaaaa
    21661 gatctagaga tttatccaac aagactagag gaatgtgatg atggtcgcag taaaacagga
    21721 aatcaatact gtgattgaca tcttcaggat aataaataat cccagcatag tctgacaggg
    21781 tgtctaacct tactgtactg gccaattgaa ccatgagacg gtaacctgtt tcagcaatgg
    21841 aggtcataat tcccttagca taatctccca attcataatc agaagtaaaa ggaagaatca
    21901 acaataaatc cttattcgct tcaggggacg tcacttgctt ttcagctacg aagcttcctt
    21961 tcccacgact tctcgtaatc aagccttctt gctccagttc tacaagcgct cgcttagaag
    22021 taatacggct aaccgagaac tgttctgaca attctttttc agtaggtagg cggtcaccaa
    22081 tttgcaaacg acctgatgct atctgctctt taatgaaatg atatatcttt ttgtataata
    22141 attttgtcat tctttactcc cgttatcagt atagcatgtc aaaaaagagt tgtaaacctt
    22201 ttcaaaaact tatagacaaa gtttaactta tcatttataa tgaaaccgaa gataaatgat
    22261 atatcatttt ggaggatatg atatgacacg accaatccca actagcgtca gccaatttat
    22321 ggccaaggtg gaaagccttt gtggagacca acaccctgat tgggctctta atttcaaaac
    22381 cagctttacc aataccttag agacaaccct aaaaacctac gaagatggca ctagctttct
    22441 cttgacagga gatattcctg ccatgtggtt acgtgattcc actgcgcaaa tgaaacctta
    22501 cctcttcctc gccaaggaag atgaagaaat tagaaagatt attgccggtt tggtcaaacg
    22561 ccaattccgc tacatttgta tcgatcccta tgccaatgcc ttcaacgaag aagctaacgg
    22621 aaaaggacac caaacagatc gtacccaaat gaacccttgg atttgggaac gcaaatatga
    22681 gattgattcc ctctgctacc ctattcagct agcttacttg ctctatcgtg aaacaggaag
    22741 caccgatcag tttaatgacg attttcatcg tggggtagaa ctcattcttg acctatggac
    22801 agttgagcaa gaccacgccc agtctcctta cttgtttgaa cgcgatactt ggcgcaaaga
    22861 agatacccta acacatgctg gaaaaggaag tcctgtcgca ccaacaggga tgacctggtc
    22921 aggctttaga ccaagtgatg atgcttgtca atatggttac ctcattcctt caaatatgtt
    22981 tgcagtggtt gtgctttcct atctagaaga tttatataac aacctttttc acaatgaacc
    23041 agtagctact cgtgccaagc agctgaaaga agctattcaa tcgggtattg cagaccatgc
    23101 ccttgttcaa aacagcaagg gagaaaccat ttacgcttat gaagtggatg gtctgggaca
    23161 gttttccatc atggatgatg ctaatattcc aagcctcctt gctgcacctt accttggttt
    23221 ttgcgctaaa gatgacccta tctatctggc aacgcgccgc accattctca gccaagaaaa
    23281 tccttattat tatcagggaa atgcagcagc aggcatcggt tcttcccata caccagaaaa
    23341 ttatatctgg catatcgccc ttgccttaca aggattgacc gctcttgacc aagacagtaa
    23401 aaaagagatg cttgatttgc ttgtggcaac agatgctggt acccacttaa tgcacgaggg
    23461 tttcgatgtc aatgatcctt atcaatacac tcgtgagtgg ttctcttggg ctaacatgat
    23521 gttttgtgaa ctacttcttg actatcttgg ctttagcatt actccttaga aagtgaggcc
    23581 actatggcaa ctaaaaaagt acatattatt tcacacagtc actgggatcg cgagtggtac
    23641 atggcttacg aacaacacca catgcgtctg attaacttaa tagatgacct gttagaagtt
    23701 tttcaaacgg atcctgattt tcatagtttt catttggatg ggcaaaccat tatcctagat
    23761 gattatttgc aagtacgccc cgaacgagaa cctgagatta gacaagccat tgcttcggga
    23821 aaactccgta tcggaccttt ctatatctta caggacgatt ttttgaccag cagtgaatcc
    23881 aatgtgcgca atatgctgat tggtaaggaa gattgtgaca gatggggcgc tagtgtgcca
    23941 cttggttatt ttcctgatac ctttggaaat atgggacaaa caccacagct gatgttaaaa
    24001 gccggcctac aagctgctgc ctttggtcgt ggcattcgtc caactggatt taacaatcaa
    24061 gtagatacca gtgaaaaata cagctcacaa ttctctgaaa tcagttggca aggcccagat
    24121 aacagtcgta ttcttggact cctcttcgcc aactggtaca gcaatggcaa tgagatcccg
    24181 acaacagaag ctgaggcgcg tcttttttgg gataaaaaac ttgctgacgc cgaacgcttt
    24241 gcctcaacca agcaccttct gatgatgaac gggtgtgatc atcaacccgt acaacttgat
    24301 gtcaccaagg caatcgcctt agccaaccaa ctctatcctg attacgaatt tgtgcattcc
    24361 tgctttgaag attacttgtc tgatctcgca aatgatttac cagataacct ttcaaccgtc
    24421 caaggagaga ttaccagtca agagacagat ggctggtata ccctagctaa cacggcttct
    24481 gctcgcattt acctcaaaca ggctaatacc agagtctctc gtcaactcga aaacatcacc
    24541 gaacccttag cagcaatggc ttatgaggtg acaagtacct accctcacga ccaactgcgt
    24601 tacgcttgga aaaccctcac gcaaaatcac cctcatgatt ctatctgtgg ttgtagtgtt
    24661 gatagtgttc atcgggaaat gataaaccgt tttgaaaaag cctatgaagt cggacactat
    24721 ttagcaaaag aagctgccca gcaaattgct ggcgcagtta acacgagtga ctttccaaca
    24781 gatagccaac ctttcctctt atttaatacc agtggccatt ccaaaacaag tgttactgag
    24841 ctcaccctga cctggaaaaa atattatttt ggccaacgct ttcctaaaga ggtttaccaa
    24901 gaagctcaag agtatttggc aggactctcc caatctttcc aagtcattga tgtatcagga
    24961 aacactatct cagaagcaga cattttggac acaagcatcg cctttgacta cgatttgccc
    25021 aagagaacct tccgagaacc ttatttcgcc atcaaagtga gattacggct accaataact
    25081 ctccctgcca tgtcttggaa agccttagca ttacaactag gaaacgagac agctccttca
    25141 gcaacggtcc cactctacga cgatagtaat cagtgccttg aaaatgagtt tctaaaagtt
    25201 atgatacaaa ccgatggccg tctaaccatc accgataaac agtcaggggt gagctatcaa
    25261 gatctcctac ggtttgaaga ttgtggcgat attggaaatg aatacatttc ccgccaacca
    25321 actcatgacc aacctttcta tgcggaccaa gggaccacca agcttaacat cattagcaac
    25381 accgctcaag ttgctgaact tgaaatccag caaacctttg ccattcctat ctccgcagat
    25441 aagctcttac aggctgagat ggaagctgtc attgacatca cagaacgcca agcaagacgt
    25501 tcacaagaaa aggctgagct aaccttaaca acccttatcc gtatggagaa aaacaatcct
    25561 cgcctccaat ttaccacacg ttttgataac caaatgacaa atcatcgctt gcgcgtccta
    25621 ttcccaacgc accttaaaac agaccatcat ctagctgaca gtatttttga aactgtcaaa
    25681 cgtccaaatc atccagatgc caccttttgg aagaatccaa gtaacccaca gcaccaagaa
    25741 tgctttgtga gtctctttga tggtgaaaat ggagtcacta ttggtaacta tggcctcaac
    25801 gaatatgaga tcttaccaga taccaacacc attgccatca ctctcttacg ttctgttggt
    25861 gaaatgggcg actgggggta tttccctact cctgaagccc aatgcctcgg caaacacagc
    25921 ctatcttata gttttgaaag catcactaag caaacacaat ttgccagcta ttggcgggca
    25981 caagaaggcc aagttcctat cctagtcgaa caaaccagcc atcataaggg aagcttaaga
    26041 gcagagcata gctatgtcac tggggcagac gatcaagtcg ctctaacggc tttcaaacgt
    26101 cgcttagcag acaatgccct tatcacgcgc agctacaatc tttcaaacga taaaacttgt
    26161 gactttagcc taagcctgcc aaactacaat gctaaggtca ctaatttgtt agaaaaagac
    26221 agcaagcaaa gcacacccag ccaacttggc aaagctgaaa ttttaactct agcttggaag
    26281 aaacaataaa ctattatcat tagtaataga ttaacatatt caaaaagagt ctgggactat
    26341 tgtctcagac tcttaagttg attttgagtg attattaata cctttaacat cagtcaggct
    26401 tagctatttc tattaactgg gttaaaatgc catcatgaat ctgcgagcta attgtgagat
    26461 agtcataacg atttaaaatg gtcgtgacat tccttaagtc aaattgccag tcatctcttt
    26521 ttttcaaagc cggtgtagca aacacgcttt gactatcatt ttgtccttgt ttagtactgc
    26581 tttgaatgac tatgacaagt ttatggtttt gatccagaaa tgctattgat aagcaaggtt
    26641 gctttgtctc caaactcatt tctatagcat tatctaacaa aaatgacaat agcttagtga
    26701 aatctactac ttctagaaaa aacgttgcaa tacaattagg aatcgatacc tcaaccttaa
    26761 gcttcttatt ttttgcctca attaatttag ctaaaacaat atgtctgatt gcttcaactt
    26821 gtaatttatc taaacaagtt aaatcaagtc tgtcatcttt agcattattc ttttcagcat
    26881 tcaagatacc ttcataagta agtctcacaa tagagatatc attttgttcg attccaatct
    26941 taagacgact ggacacggta gttaatagct gcctaagctc ttgaatatct tgatacagca
    27001 atccaaggtg ctgactataa ttaatcagct cacgatattc tttttgttgc acaattgcct
    27061 caatagagtt ttgttttaca taacgctcta aatgaattaa taaagtaaga aacaaaatga
    27121 aataaaagag cactatgatt gaacgagcag tcgttgtagc aagtcctgat aaagaaggcg
    27181 acatcacatc tatcccttgg acacacaagt aatatagagc catcgaagag ttagtaaaca
    27241 ctaatagttt actagctcta tgtgattgaa aatattggcg ccaggttgaa aaatcgaaat
    27301 gaaaaacttg aatgcagcga taaatcaaaa aaatactaaa gcaagtaatg ctgtaaatca
    27361 gtagtgtatt atgtttaaca actgaataag gcataccaaa caatggcata acaaaaaata
    27421 caataaatcg cttaaccaaa ctaatagaca ctagtggaaa aaaggcataa aacattaaaa
    27481 acttagcttt tattgattta ccatagtata gggctataag cgcaaagaaa agaaactgag
    27541 ccaacatcac aacatcaaga atgaaatggt ctggaaagaa agcccaaaaa actacttgaa
    27601 acaaaaccat actaagtgat aaataaaaaa tatt
//
LOCUS       DATUYR010000021        26099 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.021, whole genome shotgun sequence.
ACCESSION   DATUYR010000021 DATUYR010000000
VERSION     DATUYR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26099
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.021"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            41..1102
                     /locus_tag="VCI33_000811"
     CDS             41..1102
                     /locus_tag="VCI33_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEP2831746.1"
                     /translation="MVQLVTKSNRPIGTAYISKQNKGIGWYLGPEKIDLSISYFVSLF
                     SVAKAKRQDFAQSDETNAYRLFNQEGDGFGGVTIDLYKDFAVFSWYNAFVYDKKEMIM
                     EAFQQVFPEVKGAYEKCRFKGPDTETAHLYGELAQETFSILENGIAYQVFLNEGLMTG
                     VFLDQHDVRRALVDGLAMGKSLLNLFSYTAAFSVAAAMGGAIETTSVDLAKRSRELSL
                     AHFEHNQLNLASHHFVVMDVFEYFKYAKRKKLIFDVIVIDPPSFARNKKQTFSVSRDY
                     HKLITEALDILSPKGTIIASTNAANMTVSQFKKQIIKGFGSRRPESMTLQQLPSDFTI
                     NKADERSNYLKVFTIKVRE"
     gene            1099..1785
                     /locus_tag="VCI33_000812"
     CDS             1099..1785
                     /locus_tag="VCI33_000812"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEP2831747.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            1879..3045
                     /gene="aroC"
                     /locus_tag="VCI33_000813"
     CDS             1879..3045
                     /gene="aroC"
                     /locus_tag="VCI33_000813"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEP2831748.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            3106..3447
                     /locus_tag="VCI33_000814"
     CDS             3106..3447
                     /locus_tag="VCI33_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEP2831749.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3669..5021
                     /gene="gorA"
                     /locus_tag="VCI33_000815"
     CDS             3669..5021
                     /gene="gorA"
                     /locus_tag="VCI33_000815"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEP2831750.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKDKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(5109..6377)
                     /locus_tag="VCI33_000816"
     CDS             complement(5109..6377)
                     /locus_tag="VCI33_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP2831751.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(6407..6847)
                     /locus_tag="VCI33_000817"
     CDS             complement(6407..6847)
                     /locus_tag="VCI33_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831752.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSATEGTQLPNATGAEDANTGLPTNS"
     gene            7082..8224
                     /locus_tag="VCI33_000818"
     CDS             7082..8224
                     /locus_tag="VCI33_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP2831753.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            8236..9450
                     /gene="thiI"
                     /locus_tag="VCI33_000819"
     CDS             8236..9450
                     /gene="thiI"
                     /locus_tag="VCI33_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEP2831754.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAIAVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            9488..10780
                     /locus_tag="VCI33_000820"
     CDS             9488..10780
                     /locus_tag="VCI33_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEP2831755.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNKLAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    10885..10959
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            10994..11308
                     /gene="rplU"
                     /locus_tag="VCI33_000821"
     CDS             10994..11308
                     /gene="rplU"
                     /locus_tag="VCI33_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEP2831756.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            11320..11646
                     /locus_tag="VCI33_000822"
     CDS             11320..11646
                     /locus_tag="VCI33_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEP2831757.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            11674..11967
                     /gene="rpmA"
                     /locus_tag="VCI33_000823"
     CDS             11674..11967
                     /gene="rpmA"
                     /locus_tag="VCI33_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEP2831758.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            12325..13239
                     /locus_tag="VCI33_000824"
     CDS             12325..13239
                     /locus_tag="VCI33_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP2831759.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLSDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            13236..13694
                     /locus_tag="VCI33_000825"
     CDS             13236..13694
                     /locus_tag="VCI33_000825"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEP2831760.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            13684..14574
                     /locus_tag="VCI33_000826"
     CDS             13684..14574
                     /locus_tag="VCI33_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP2831761.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDTLAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            14969..15490
                     /gene="pyrR"
                     /locus_tag="VCI33_000827"
     CDS             14969..15490
                     /gene="pyrR"
                     /locus_tag="VCI33_000827"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEP2831762.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            15506..16765
                     /locus_tag="VCI33_000828"
     CDS             15506..16765
                     /locus_tag="VCI33_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEP2831763.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKVIGNDWIDKILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            16826..17761
                     /locus_tag="VCI33_000829"
     CDS             16826..17761
                     /locus_tag="VCI33_000829"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEP2831764.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            17805..18887
                     /gene="carA"
                     /locus_tag="VCI33_000830"
     CDS             17805..18887
                     /gene="carA"
                     /locus_tag="VCI33_000830"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEP2831765.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            19133..22309
                     /gene="carB"
                     /locus_tag="VCI33_000831"
     CDS             19133..22309
                     /gene="carB"
                     /locus_tag="VCI33_000831"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEP2831766.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPTLDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQDLQMHVEHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
     gene            22516..23787
                     /locus_tag="VCI33_000832"
     CDS             22516..23787
                     /locus_tag="VCI33_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP2831767.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVAVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            23787..24497
                     /locus_tag="VCI33_000833"
     CDS             23787..24497
                     /locus_tag="VCI33_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831768.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            24509..25729
                     /locus_tag="VCI33_000834"
     CDS             24509..25729
                     /locus_tag="VCI33_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831769.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGEGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
BASE COUNT         8186 a         4382 c         5605 g         7926 t
ORIGIN      
        1 agatgaaaaa gatttttcta atataaagga aaaaaatcag ttggttcaac tggtgaccaa
       61 gtccaatcgt cccattggaa cagcatatat ctctaagcaa aataaaggaa ttggttggta
      121 tctaggacca gaaaaaatcg acttgtctat ctcttatttt gttagtctat tttcagttgc
      181 taaggccaaa agacaagatt ttgctcaatc ggacgagacc aatgcttatc gcctttttaa
      241 tcaagaagga gacggttttg gtggagtaac aattgatctt tataaagatt ttgcagtctt
      301 ttcatggtac aatgcctttg tctatgacaa aaaagaaatg attatggagg cttttcaaca
      361 ggtttttcct gaagtcaaag gagcttatga aaagtgtcgc tttaaaggtc cagatactga
      421 aactgctcat ctgtatggtg agctggcaca agaaaccttt tcgattttag aaaatggcat
      481 agcctatcaa gtcttcttga atgagggctt aatgacaggt gtttttctag accagcatga
      541 tgttagaaga gcactagtag atggtttagc tatgggcaag tcccttctta acctattttc
      601 ctatacggct gccttttctg tagcagctgc tatgggtggt gctatcgaga ccacttctgt
      661 ggacttggct aaacgatcga gagaattatc actagctcat tttgagcata accagttaaa
      721 cttagcctca catcattttg tggtgatgga tgtttttgaa tacttcaaat atgccaaacg
      781 gaaaaaatta atctttgatg tgattgtgat tgacccacca agctttgcta gaaataaaaa
      841 acaaactttc tctgtgagca gggactacca caaattaatc acagaggcct tggacatatt
      901 gagtcctaag ggaactatta tagcttcaac aaatgctgct aatatgactg ttagccagtt
      961 caaaaagcaa attatcaaag gatttggaag tcgtcgtcct gagtctatga ctctccagca
     1021 gcttccgagt gactttacta tcaataaagc agatgaaaga agtaactatt taaaggtatt
     1081 tactataaag gtacgagaat gagaattgta gcaccagtca tgccaagaca ttttgacgag
     1141 gctcaagcca ttgatatttc taaatatgaa gatgttaatt taattgaatg gcgagcagac
     1201 tttcttccta aggatgagat tgtcgcagta gcaccagcta tttttgaaaa atttgccgga
     1261 aaagaaatta tctttaccct tcggaccgtt caagaagggg ggaatattac cctttcaagt
     1321 caggagtatg ttgatattat caaagaaatc aatgctattt ataatccaga ctatattgac
     1381 tttgagtatt ttacgcacaa gtcagtattt caagaaatgc ttgattttcc aaatcttatt
     1441 ttgtcttatc acaactttga agagactcct gaaaatttaa tggaagcttt ttcagaaatg
     1501 accaagttgg caccgcgtgt ggttaaaatt gctgtgatgc cccagagtga acaagatgtt
     1561 ctagatttga tgaactatac cagaggcttt aagacgctca atcctgagca agaatttgca
     1621 actatatcta tggggaaatt aggaagattg tctcgttttg cgggagatgt cattggctct
     1681 tcctggacat acgtttcatt agatcatgtt agcggtccag gtcaagtgac tctcaatgat
     1741 atgaaacgta ttattgaggt cttagaaatg gatatctcta actagtgaat ggtctaaaaa
     1801 tattatttct acaatagtta agaaatgatg tttttagatt aatgttttta aaataagtgg
     1861 aaccaaaaag gagaaaaatt gcgttattta acagcaggag aatctcatgg gccatcctta
     1921 acagctatta ttgaaggtat accagcagga ttaactcttc atccagcgga tattgatcac
     1981 gaattacagc gacgtcaagg aggttatggc cgtggtgcca gaatgtctat tgagactgat
     2041 cgggttcaaa tatcctcagg agttcgtcat ggcaaaacaa caggggcccc cattacctta
     2101 acggtaatta ataaggacca ccaaaaatgg ctagatgtca tggctgtagg agacattgaa
     2161 gagactctca agttaaagcg tcgtgttaag catcctagac caggacatgc ggatttggta
     2221 ggaggtatta aatatcattt taacgattta agagatgctt tagagcgttc ctcggctaga
     2281 gaaacaacga tgagagtggc tgtaggcgct gttgctaaac gtatcttagc tgaacttgga
     2341 attgatatgt tacatcatat cttaatattt ggtggtataa cgatcacaat accttctaag
     2401 ctctctttta gagagttgca agaaagggct ctccattcag agttatctat tgttaatcca
     2461 aagcaagaag aagaaattaa aacgtatatt gataagataa aaaaagaagg ggatactatt
     2521 ggtggtatta tcgaaacgat agttcaaggg gtgccagcag gtcttggatc ttatgtccaa
     2581 tgggataaaa aattagatgc caagctggct caagcagttc tttccattaa tgcttttaaa
     2641 ggggtggaat ttggagttgg gtttgatatg ggattccaaa agggctctca ggtaatggat
     2701 gaaatcacct ggacacctac ccaaggctat ggtcgtcaaa caaaccactt aggtggcttt
     2761 gaaggtggca tgaccacagg acaacctctt gttgttaaag gggttatgaa gcccattcct
     2821 actttgtata aaccactcat gtcggtagat attgatagtc atgaacctta taaagcaacg
     2881 gtggaacgct ctgatccgac ggccttacca gctgcagggg ttatcatgga aaatgtggtg
     2941 gcgacggtgc ttgccaaaga gattttagag actttttctt ctactaccat gtctgaattg
     3001 caaaaagctt tttcggacta tcgtgcctac gtcaagcaat tttagatagc gagatactgg
     3061 cttttatggt atcatacagg tattaatgaa caaaaggaga ctgtcatgtc acaagaaatt
     3121 tatgactatg ctaatcaact tgaaagagca gttcgtgctc tgccagaata ccaaaaagtg
     3181 ttagaagtaa aagaggccat tcaagcagac gctagtgcta gcgaactgtt tgatgagttt
     3241 gtcgctatgc aagaaaaaat ccaagggatg atgcaatcag gccaaatgcc aacggctgaa
     3301 gaacaaacaa gcattcagga acttagccaa aagattgaag ctaatgacca gctaaaagct
     3361 tattttgaag cgcagcaggc cttatctgtt tacatgagtg atattgagcg tattgtattt
     3421 gctccgttga aagatttagt caagtaagcc aattaagata ggaaaaaaaa cagattgctg
     3481 gcagctcgtc aggatctgtt ttttagtgtt tattttcttt gtatcagaaa gttgagcctc
     3541 tttcttattt gtgatatagt caaataaatt gaccaaaata gcaggccagt agtggcttta
     3601 atgaaggcaa gttgtctgct aactggctat gttaaaacat cagtattaga aagtaaaagg
     3661 ttataaaaat ggttattcct tatgattata ttgttattgg tggagggagt gcaggtattg
     3721 cttctgcgaa cagggcagcc atgcatgggg ccaaagtatt gttagctgaa ggtaaagaga
     3781 ttgggggaac ctgtgttaac ctaggctgcg ttcctaaaaa agtcatgtgg tatggcgctc
     3841 aagtagcaga tattttaggg acttacgcta aggattatgg atttgacttt aaagacaaag
     3901 catttgattt taaacaattg aaagcaaatc gtcaagctta tattgatcgg attcatgctt
     3961 cttatgagcg aggctttgag cagaatggtg ttgaccgtat ttatgattat gctgttttca
     4021 aagatgctca tacggtagaa attgcagggc agctttatac cgcaccgcat attttgattg
     4081 ccacaggagg acatcctgtt tttccagata ttgagggggc acaatacggt atcagctcgg
     4141 acggcttttt tgccttagat gaggtaccaa aacgtacggc tgtggttggg gcaggctata
     4201 ttgctgtgga acttgccggt gttttgcatg ctttggggtc gaagacggat ttgtttatcc
     4261 gtcacgatcg acctttaaga agttttgaca aagccattgt ggatgtttta gttgaggaaa
     4321 tggcagttgg tggtccttgc ttacaccctc attcagaagt ggcaaaggtt gtcaaaaata
     4381 ctgatgagtc cttgaccctt tacctcaaag atggccaaga agttgaggtt gaccagctca
     4441 tttgggctat cggtcgcaag cctaatctag aaggttttgg tcttgacaaa actggagtga
     4501 cgttaaatga taaaggttat attgagacag atgcctatga aaacacgtct gtcaaaggta
     4561 tttatgccgt gggagatgtt aatgggaaac tggctttaac tccagttgct gtggcagctg
     4621 gacgtcgcct atcagagcgt cttttcaatg gcaaaacaga cgagaaattg gactaccaaa
     4681 acgttgctac tgtgattttt agtcaccctg ttattggctc tgttggcttg tcagaagaag
     4741 cagcagtcaa acagtatggg caagaagcag ttaaaacata tcaatcacgt tttacgtcta
     4801 tgtttactgc tatcactaac catcgccaac cttgtctgat gaagttggta acagtcggag
     4861 ataccgaaaa aatagttggt cttcacggaa tcggctatgg ggttgatgaa atgattcagg
     4921 gctttgcagt agccatcaaa atgggagcaa ccaaggcgga ttttgataac accgttgcta
     4981 ttcacccaac tggctcagaa gaatttgtca ccatgcgcta aaaaatgtag cctaaggcaa
     5041 taaaaaatga tgttaacagt ttttgaggaa agtcttctaa actgctagca tcgttttttg
     5101 ttaggtgatt aggggaatat gtttttaaag atatttgcgt tgctcagaca atattgcctt
     5161 acctcggaga taaaataaag tgaccctgta acaacaaaga gttcatcaga tgttacggaa
     5221 ccttgtaacc agtctctgta atcttttacc tgtgggtatt tatcaggata atcctctaaa
     5281 agatttgctt cagggaaatc aaaagtcgtg acagatactt caaaagtatc tagttgagcc
     5341 agcaagtcgg ctaaaggctt acgtttgaga cctgcaaaga gaatatgaat aggacgtttg
     5401 ggaaattctt cttgcagtag agactttaat ttagcaatgg aatctggatt gtgagcgccg
     5461 tctaatagaa gattaggttg aataaattcg gaccggccag gccaagttgt tgcctcaatt
     5521 cctttttgaa tcgtttttgg agatatttta ggaaaggatt ttctaagtaa ttgagctgtc
     5581 ataatggcta aacaggcatt cgctttttga tgttgaccta atagtttgag acggagtttg
     5641 gttaaattca agttatcgtg ttgaaaggaa aacgtctttt ctgaaggatt gagcaaaaaa
     5701 tcttttccta attgatataa gcgtgaaccg gctttttggg taacttgttg aaagacttga
     5761 ctagcctcct gctcaagttg accgataata acgggttctt tagctttgat aacttctgct
     5821 ttttggtgag caatttctgc taggctataa cctaaacgtt cttggtgatc aaaactaata
     5881 gaagggcata cgacggccag agcatgaaaa acattggttg cgtcggtttt cccaccaatc
     5941 cctgcctcaa taatggcaat atcaacaggt ttaagattgg caaagtagca aaacattatg
     6001 agagtcacta actcaaactc ggtaggtcta tcccaatttg tttcaaggga cattttagtg
     6061 agaatgggtc taatcacgct cacacattgc gccaattcta ggtctgaaat gggctggcca
     6121 ttaaggcaaa tccgatcgtg aaaagagata ataaaaggag atgtaaaggt tccgacctga
     6181 taaccagaag ttgacaggat atgctgtaag taggctgtca cagagccctt gccgttagtt
     6241 ccaacaacgt gaatagcagg gaatttgtct tgtggccggc ctaatgcctc aagtaaccaa
     6301 gccattctgg ccaaatcggt cttgcgacca ttagccttaa aggtatgaat ccaggaaatg
     6361 ctatcatagg tagtcattac tatttcttgt ccttctaaca atgcttttaa gagttcgtag
     6421 ggagaccagt attagcatcc tcagctccag ttgcattagg aagttgcgtc ccttcagtgg
     6481 ctgatggtgt ctcaggagct gtaggagttg tctcgttgtg agaaggtgtg gtagaagatg
     6541 acggaagagt tgtcttatta tcatctgtgg tttctgaagt acctgtatgg ttttgattac
     6601 cctgagaaga ctctttttca ataatcttga caatggtatt actcgattct ttgggtgtat
     6661 cattagaggt cttatctggt acaggctgca gtgctcttgt aatggtaatg gttgccacaa
     6721 tgatactcaa aatactacca ataagtgcaa tggatttttg gaaagctgta aaacctgttt
     6781 taatatgctc ggtagggaca cctcctgatt tacgaggttt ttgttgacgg gaataacgtg
     6841 atgacatatt tttctccttt aaaatcatcg taatgccaag attaaggagg aggaaataga
     6901 agtcctagct cctctttaac acttcatcta tcagtataat ctagaaaaaa agtgaagtca
     6961 acggtttacc ttatgtctag gacaaggaag tcctttgggc ttattgtaat acgaatattc
     7021 ttgatttcat tttagctatt ttgtgataga ataaagtatt gagtaaaaat gaaggtgaca
     7081 catgatttat tttgataatg cagccacaac cataccttat ggagaggctc ttaaaactta
     7141 ccaagaagta gcaacaaaaa tatatggcaa tccctcaagt ctgcaccaat tagggacaaa
     7201 tgcgtctcgc attttagaag cttcccgtaa acagattgct ggtttgttgg gtgttaagtc
     7261 agaagaaatc ttttttacgt ctggtgggac tgaaagtgct aattgggcta ttaagggaat
     7321 tgcttttgaa aaaactgctt ttggcaaaca tattattatc tcagccattg aacaccctgc
     7381 cgtgagtgaa agtgtcaaat ggcttctcac acaaggattc gaagtttctt atgctccagt
     7441 gacaacacag ggagttgttg atgtgaatgc tttagctgag ctgattagac cagacaccat
     7501 cttaatctcg attatggctg ttaataatga aatgggagct atccaaccga ttagagccat
     7561 ttcaaaccta ttggctaacc aaccaacgat cacctttcac gttgatgctg ttcaagccat
     7621 tggtaaaata ccactctgtg attatatgac taatcgtgtg gatttggctt ctttttcagg
     7681 gcacaaattt catagtgttc gtggtgttgg cttcctttat aaaaaagcag gcaagcgact
     7741 caatcctctc ttgtcaggcg gcggccaaga gcaggagtta cgttcaacca ctgaaaatgt
     7801 tgcaggaatt gctagcatgg ccaaagcatt aagaatagtc actgaaaaac aagtgagcgt
     7861 cttacctaaa ctaacagcga tgcgagatgt gatttacaaa gctttgtccg cttacccaga
     7921 tgtgacagta ttttcagctc aagaaggctt tgcgcccaat attttgacct ttgggattag
     7981 aggggttcga ggagaagtta ttgtccacgc ctttgaaaaa tatgagattt atatttcgac
     8041 cacaagtgca tgttcgtcca aagcaggtaa gccagcagga agcctagtgg ctatggggat
     8101 tcctgtaaaa actgcccaga cagccgttcg tattagtcta gacgatgaca acgatatggg
     8161 acaagtagag cagtttttga ccatattcaa acagatttat gataaaacac agaaagttag
     8221 gtaagattag gtaagatgga ctattcagaa attatggttc gccatggtga actgtcaact
     8281 aaagggaaga atcgcatgcg attcattaac aaacttaaaa acaatattca agacgtactg
     8341 gctcctttcc cagccattgc tgtccgttca gatcgtgata ggacccatgt gtctctaaat
     8401 ggcacagatt atcagcctat tgtagaagct ttaaagctgg tgtttggtgt tcaagctcta
     8461 tctcctgttt ataaacttga aaaaagtgtc cctcttttgg taacagctgt tcaagatatc
     8521 atgacttctc tttatcgtga tggactgacg tttaagattg ccactaagcg tagtgatcac
     8581 gcgtttgaat tggatagtcg tgagcttaac agtcttcttg gtggagctgt ttttgaggtt
     8641 ttaccaaata tccaagccca gatgaaacat cccgatgtga ctctcaaagt cgaaattcgt
     8701 gatgaagcag cttacatttc ttatgaagag attaagggag caggcgggtt accagtagga
     8761 acgtcaggga aagggatgct gatgttatcg ggcggtattg attcaccagt agctgggtat
     8821 ttagccctta aacgtggcct cgatattgaa gttgttcatt ttgctagccc tccgtacacc
     8881 agtccaggtg ccttggctaa agcccaagac cttactcgtc ggttaactcg ttttggtgga
     8941 aatatccaat ttatcgaggt tccttttaca gaaattcaag aagaaattaa aaataaggca
     9001 ccagaggctt accttatgac attaacccgc cgctttatga tgcgcatcac agatgccatt
     9061 cgtgagcaac gtaagggtct tgttattgtt aacggtgaga gcctaggtca ggttgctagt
     9121 caaacccttg aaagcatgca ggccattaat gcggtaacat caaccccaat tattagacca
     9181 gtcgtgacaa tggataaact tgaaattatc gagatggctc aggcgattga tacttttgac
     9241 atttctattc aaccgttcga agattgctgt actatttttg caccagaccg tcctaaaacc
     9301 aaccctaaac taggtaatgc tgaaaaatat gaagaacgct ttgatattga tggtcttgtc
     9361 cagcgtgctg tgtcaggaat tgttgtcaca gaaatcacac ctgaaatcgt caatgatgag
     9421 gttgaaaatc tcattgatgc tttactttaa aagaaaagat caagtgtaaa tcttttagta
     9481 gtcagtaatg atactattga aaagcctcta taagggcttt tttgctgcaa tgaaggagga
     9541 aaattttgag gcaagatgtt ataatagaga aaaaggaggg tgtgtcttga ttaaaactat
     9601 gcaatacaaa aaaacgatgg caacagttgt tgctcttatc gtcgcacttt tgctatttgg
     9661 cttgatttat gatttacttg gggttcaaaa aaataagcta gcagctcaaa aatctgctca
     9721 gccaaaggtc aaaacagcgc gtgttgtagc taatggcgac attttaattc atgatattct
     9781 ctacatgagt gctagaaaag ctgacgatac ctatgatttc actccttatt ttgaatatgt
     9841 taaggactgg attagcggtg ctgacttagc tattggagat tatgaaggga ccattagccc
     9901 agattatccc ttagcaggct atcccttatt caatgctcca gaagagattg caggtgctct
     9961 gaaaaatact ggctatgatg tagtcgattt agcccataat catatcttag attcccaatt
    10021 agacggtgcc cttaacacta aaaaggtttt tcatcaatta ggtatagaca gtattggcat
    10081 ctatgacaag gatcgttcaa aagaatcctt cttaattaaa aatgtcaatg gtatcaaaat
    10141 tgcaatttta ggttattctt atggttataa cggcatggag gccacactta gccaagaaga
    10201 ctatgagaaa cacatgtctg atttagatga agctaagata aaaaaagaac ttcagctagc
    10261 tgaaaaaaag gctgacgtga ccattgtcat gccacaaatg ggaacagaat atgccttaga
    10321 accgacagca gagcaaaaag aactttacca caaaatgatt gattggggag ctgacgtcgt
    10381 cctaggaggc catccgcatg ttattgaacc ttcagagaca gttataaaag gtaggcaaaa
    10441 gaaattcatt atttactcta tgggaaattt tatttcaaat cagcggcttg aaaccgtaga
    10501 tgatatctgg actgagcgtg gcctattgat ggaccttact tttgagaaaa aagataacaa
    10561 aacaaaaatt aaaacagttg aggcccatcc aaccatggtt ttagccaaag gtaaaggcat
    10621 tgtgggtaaa gaaggttttg aactatacaa ttatcgaaca atggttttag aagattttat
    10681 caaaggtgga aaataccacg acaagattga tgaggaaacc aaagcaaaag tagcacttgc
    10741 ttatcaggaa attaatgatt tagttaacct gaaatggtaa tgtttggtgg tttagcatct
    10801 aacgataaaa aatagagatt aaacttaatt tttccttgat aaaaagcagt tatggtgcta
    10861 aaatagtaac gttgactaaa tgcacatcct gtgcaaccgc gcgaaaatcg ttgtcaagta
    10921 gaccaagtct cacgattcca ggcgagtctt cacagaggga aacctcaaac aaaaatttta
    10981 taggaggtgc atcatgagca catacgcaat catcaaaact ggtggaaaac aagttaaagt
    11041 tgaagtaggt caagcaatct acgttgagaa aattgacgct gaagctggcg cagaagtcac
    11101 ttttaacgaa gttgttcttg taggtggtga caaaactgta gttggtactc cagttgttga
    11161 aggagctact gtcgttggaa ctgttgaaaa acaaggaaaa caaaagaaag ttgtaacatt
    11221 caaatacaaa cctaaaaaag gtagccaccg taaacaaggt catcgtcaac cttacactaa
    11281 agttgtcatc aacgcaatca acgcttaatt tactagctta tgattaaagc aatttttact
    11341 cgccaaaaaa atggtcaact gagcagtgtt acactgacag gtcatgctgg tagtggtaaa
    11401 catggctttg atattgtttg tgcctctgtt agtactctag ccattaactt tgttaattct
    11461 cttgaggtgt tagcagattg ccaagcatta gtagatctta atgatgttga aggtgggtat
    11521 atggctatta caataccacc acatgacaac aaggaggaag ttcaattatt atttgaatct
    11581 tttcttctag gaatgactag tttggcgaag gattcttcga aatttgtcaa cactcaggtc
    11641 atctaaattt aatttaagag aggaaacaac attatgttaa aaatgaatct tgctaacttg
    11701 caacttttcg cccacaaaaa aggtggaggt tctacatcaa acggacgtga ctcacaagct
    11761 aaacgccttg gtgctaaagc tgctgacggt caaactgttt caggtggatc aatcctttac
    11821 cgtcaacgtg gaactcatat ctacccaggt gtaaacgtag gccgtggtgg agatgacaca
    11881 ctttttgcta aagttgaagg tgttgtacgt ttcgaacgta aaggacgtga taaaaaacaa
    11941 gtttctgttt acccagtagc taaataattg tttaacatcg cattatataa ggctttccag
    12001 gtgtctggaa agccgatttt tttattttgg tactcatgtt gctatccata ttttaaaaat
    12061 caatgtagga agagagcttg tcagcagttt tatcttttga ggatttagta acatgagcgt
    12121 agatgttcat ggtagtttgg atagtttcat gaccaagtgt attggatttt cattgtttgt
    12181 ttctatccta ctaaagattg tagttgctta tttagagcaa tagttctaat tgtaaaaagt
    12241 cagcataata aaacaatgtc ataagatgtt gttataacgg gctttatggt ataattaagg
    12301 atactcattt taggaaggaa agcaatgaat attcaacaat tacgctatgt ggtagccatc
    12361 gctaataatg gaacttttcg agaagctgcc tctaaattgt ttgtcagtca gcctagctta
    12421 tcagtttcta tcaaagattt agaggcggaa ttagggtttc aaatttttaa tcggactact
    12481 tcaggaacag tcttgacaag tcaaggacta gttttttatg agaaagcttt agaagtagtc
    12541 aaatcttttg attcttttga aaaaaccttt tctcaagctg atctggatca aaatgaattt
    12601 tctattgcta gccaacatta tgactttttg ccacctctta ttacagcttt ttcgcaacaa
    12661 tacgatggtc atcgtgtctt tcgtatcttt gaatcaacaa ctattcaaat tttagatgag
    12721 gttgctcaag gaaacagtga aattggtatt atttacttga atgtagacaa tcaaaaaggt
    12781 ctctttcagc ggatggataa gctgggcttg gaatatgttt ctctaattcc tttcactact
    12841 catatttatc tttcaaaaac gcatccattg gctaatcgcg aagcgcttta tttgagcgat
    12901 attcaaggat tacctgcggt aaggtttacc caagaaaggg acgagtattt gtattattct
    12961 gaaaattttg tggatactag tgaatgccct cgtatttata atgtgtcaga tagggcaacc
    13021 ttgaacggta ttttagagag aaccaatgcc tttgcgacag gatcaggttt tctcgatcat
    13081 cggagtgtta atgggattaa agtgatacct ctagctgatc atattgacaa tcaaatgatt
    13141 tatgtcaaac gaaaagataa aaacctttct gttgctgggg ccacttttgt gactatttta
    13201 aaagactatt ttgataaaag aaggaaggcc ttaacatgaa aaaacgattg tttgtgctta
    13261 gcttgatcct ccttgtagct ttggatcaac ttagtaaatt ttggattgtt tctcatatag
    13321 cgcttggaga agtgaaaccc tttatcccag gtatcgtcag cttgacttac ttgcaaaaca
    13381 atggggccgc cttttccata ttgcaggacc agcaatggtt ctttgttgtc ataacggttt
    13441 tagttatcgg ttatgctatt tattaccttg ctactcatcc ccatttaaat atctggaaac
    13501 aattagctct cttgcttatt atttctggtg gaatcgggaa ttttattgat cgtttgcgtt
    13561 tagcttacgt gattgatatg gttcatttag actttgtgga ttttgccatt tttaatgtgg
    13621 cagattcata ccttaccgtt ggtgtcatat tattagtgat atgtttatgg aaagaagagg
    13681 attatggaaa ttaatgtgat aacatcgggg caacgcttgg ataaggcact agctgattta
    13741 agtcccctgt cacgtggtca agccaatgac caaattaagc aaggccttgt tttagttaat
    13801 ggtcagcaaa aaaaggctaa atatacggtt caagcaggag atgtgatttg ttttgaactc
    13861 cctaaagaag aagtcttaga gtaccaagct cagaatattc ccttggacat tatctatgaa
    13921 gatgatactc tcgccattat taacaagcca cagggaatgg tagttcaccc ttcggcggga
    13981 cacccttcag gaacaatggt taatgccttg atgtatcata tcaaggattt gtcctcaatc
    14041 aatggtgtgg ttcgtccagg aattgttcat cggattgata aggacacctc aggtctctta
    14101 atggtagcta aaacagatgc agctcatcaa gccttggcag aagaattaaa ggctaaaaaa
    14161 tctctcagaa aatacctcgc cattgtccat ggaaacctac cgaatgatcg cgggatgatt
    14221 gaagctccta taggtcgtag cgaaaaggac cgtaaaaaac aggctgttac agcaaaaggc
    14281 aaagaagcag tgacacgttt tacagtgcta gaacgttttg gcgattacag tctagtggaa
    14341 ttacaactgg aaactggtcg aacgcaccaa attcgtgttc atatggccta tattggtcat
    14401 cctgttgctg gggacccttt atatggacca cgtaagacac tatcaggtca tggccaattt
    14461 cttcatgcaa aaacattggg attaacgcat cctatgacag gaaaagaaat gatatttact
    14521 gttgaagctc ccgaaatttt tcaaaaggtg ttaaagctgc taagaaaaaa ataaacagcg
    14581 aggagtagcc attattttta gtcttatatc tttgtgtaaa gtttttaaca ttataaaaaa
    14641 tactaaaaag catttacgat aagccaggaa atatgagaag tcttttatgt tattgggata
    14701 tgcaacatat ctgttagaga tctttattta aactcttaaa gaaaagagat attccaaata
    14761 gatgctaggt attgtctttt ttacttgttt ttcttttctt tttttgctat actgattgaa
    14821 ataaaatatt cctttaaaac agtcctgtga ggctgtcaag gagacggatt aaaaacatgc
    14881 actatgatac ttaattgtgc tatattttgc tgaatctcct tgtttccaag gagatctttt
    14941 tatttgaatt tttagaggag gtttcaacat gaaaactaag gaaattgttg atgacgtgac
    15001 catgaaacgc gccattaccc gtattactta tgaaattatt gaacgtaata aacaattaga
    15061 caatgtggtt ttggcaggca tcaaaacgag aggtgttttt ctagctcgtc gcattcaaga
    15121 aagactgcat caattagaag ggttggattt accaattggt gagttagaca tcaagccttt
    15181 tagagatgac atgagagttg aggaagacac aactctgatg tcagtagata ttacaggcaa
    15241 ggatgtgatt ttgattgatg atgtgcttta tactggtcgt accatccgcg cggccatcga
    15301 taacttagtg agtttaggtc gaccagcccg tgttagtcta gctgttttgg tggatcgtgg
    15361 ccatcgggaa ttaccgattc gcgcggatta cgttgggaaa aatattccaa caagcagtgt
    15421 agaagaaatt gttgttgagg ttgtcgaagt tgatggccgt gatcgtgtta gcattattga
    15481 cccaacataa gaaggaggtt ttactgtgaa agatgtcatt tatgatgtgg aagaagtgcc
    15541 aaaggcaggt atgttagttg gtttgtcctt tcagcattta tttgccatgt ttggggcgac
    15601 ggttcttgtt cctattttag ttggaattga cccgtctgtg gccctcctat cgagtggttt
    15661 aggaacactt gcacatttat cggtaaccaa atttaaaatt ccagcctaca tgggctcaag
    15721 ttttgcttat atagcagcta tgcagttact gatgaagaca aatggtattg gtgctgtagc
    15781 tcaaggggct atgacaggag gtctcgttta cctgattgta gctttgattg ttaaagttat
    15841 tggcaatgat tggattgata agattttacc accgattgta gttggtccga ttgttatggt
    15901 cattggtcta agtctagctt ctacagctgt taatgatgtc atgttaaaaa atgggaacta
    15961 taatctgact tatcttgtta ttgggttagt caccttatta tcagttattt ttttcaatat
    16021 ttatggtaag ggaattgttg ctattgtacc gcttttatta gggctattag ttggttatgt
    16081 tgtagccctt ttagtgggtg ttctcaccgg tcaagaaatt gttgatttta ccaatgtggc
    16141 tcaggccaaa tggtttagta tcccatcagt ggaaattcct ttcttaacct acggggttaa
    16201 attctaccca agtgccattt taactatggc acccattgcc tttgttacaa tgacagaaca
    16261 ttttggacac attatggtct taaatagttt gacaaaaaga gattatttca aggatccagg
    16321 acttgaaaag actttaactg gtgatggttt tgcgcaaatc attgctggct tcttaggggc
    16381 acctccggtc acttcttatg gtgaaaacat cggtgtaatg gctctgaata aaatattctc
    16441 ggtctatgtt attgcaggtg ccgcagtgat tgccgctctc ctcagtttta tcggtaaggt
    16501 atcagcctta attcaatcta ttccaacacc agttattgga ggtatatcag ttgccttgtt
    16561 tggtgtaata gcttctagcg gtttgaaaat cttgattgaa tccaaagttg atatggataa
    16621 taagaaaaac ttattgattg ccagtgtcat tttggtctct gggattggag gattgatgct
    16681 tcaagtaaat ggtcttcaaa tttcaggtgt cgctttctca acacttcttg gcatcatctt
    16741 atatcaggta cttcctgaaa aataggaaga attgttgtta gaccacttac atagtcatca
    16801 gtcgttagtg ataaagggga taatcatgtc agttgtaaat aatcgcgttg cacttaccaa
    16861 tttagtttca atggaagcat taaccaccga agaagtttta ggtcttatca accgtggttc
    16921 agaatataag gctggtaagg ttgttatttc ggatcatcaa aaggatcttg ttgccaattt
    16981 attctttgaa aactccaccc gaacccataa gtcttttgaa gtggctgaga agaaactcgg
    17041 cttgacagtt cttgatttca atgctgatgc aagtgcggtt aacaagggag aaagtttgta
    17101 tgatactgtg ttaaccatga gtgcacttgg aacagacatc tgtgtgattc gtcacccaga
    17161 agatgattat tacaaggaat tagtagaaag tccaactatt acagcttcca ttgtcaatgg
    17221 aggagatggt tctggacaac acccgagtca atgtttactt gacttattaa ccatctatga
    17281 agaatttgga cgctttgaag gcttaaagat tgcgatcgct ggtgacttaa ctcactctag
    17341 ggtggcaaag tccaacatgc aaatcctcaa aagattagga gcagagcttt atttctatgg
    17401 acctgaagaa tggtattcag aagcctttaa tgcttacggc acttatattg ctattgacca
    17461 gattattaag gaattggatg ttcttatgtt gctgcgtgta cagcacgaac gtcatgacgg
    17521 ccatcagagt ttttcaaaag aaggttacca tcaagcgttt ggattgacac aggaacgtta
    17581 ccaacaatta aaagattctg ctattattat gcatccagct ccagttaatc gggatgtgga
    17641 aattgcagat agtttggtgg aagctcctaa agcgagaatt gtatctcaga tggctaatgg
    17701 tgtctttgtt cgcatggcta ttattgaagc cattttgaat ggtagaaata aaaactcatg
    17761 atgaaagatt aaagcatgtt tagaaggtag gaaaaggtag gaaaatgaca aaacgattac
    17821 ttattttaga agacggcact atttttgaag gagaaccctt tggtgctgat attgatgtga
    17881 ctggtgaaat tgtctttaat acaggaatga caggctacca agaatccatc actgaccaat
    17941 cctacaatgg gcaaatttta acctttacct atcctttgat tggaaattat gggattaatc
    18001 gagatgacta tgagtccatt tcacccacct gtaaaggggt tgttgtgtct gaagtcagtc
    18061 ggttggcaag taattggcgc aagcaaatga cgctagatgc gttcttgaaa ataaaaggga
    18121 ttccagggat ttcgggaatt gatacgagag ctttgaccaa aattattcgg caacacggta
    18181 ccatgaaagc tacgatggca gatgatggag atagtatcca gcatttaaaa gatcaactgc
    18241 gtgcaacagt cttaccgact aacaccattg aacaagtgtc aactaaaaca gcttatcctg
    18301 caccagggat cgggaaaaat attgtattgg ttgattttgg tttaaaacat tctattttaa
    18361 gagaattttc taaaagacaa tgtaatatca ccgttgtccc tttcaacatc actgcagaag
    18421 aagtgctcca actaaatccg gatggtttga tgctatctaa tggcccagga aacccagaag
    18481 atttgccaga ggctttagac atgattcgtg gggtacaagg taagattcct atttttggta
    18541 tttgtatggg acatcaactc tttagtctag caaatggtgc caagacttgt aaaatgacct
    18601 ttggtcatcg tggctttaac catgccgtta gagaaattgc aacgggaaga attgatttta
    18661 ccagtcaaaa ccatggttat gccgttgaac gtagtagtct acctgatact ttaatggtta
    18721 cgcatgaaga cattaacgat aagacggtcg aaggagtgaa acatagagat tttccagctt
    18781 tttcagtcca atttcaccct gatgcggctc caggacctca tgatgccagc tacttatttg
    18841 acgaattttt ggaaatgatt gattcttgga gatgtacgag taaatagtaa atcagttgca
    18901 atttcacgac tatatcacct cttgttactc tatttattaa catttacagt tgatttagta
    18961 ccctatcaaa aatattattt aatgttgctc tgtgaggcaa caaatagaga aaggaaggca
    19021 aggagaacac aagtaaataa tttagtaaaa agttaggttt tcaaagagat aaagctctta
    19081 ttgagaagtg ctgactttac cgcttcttgt ggtattcttg gtatcattaa ttatgccaaa
    19141 acgaaaagat attcaaaaaa tcatggttat cgggtctggc ccgattatta ttggacaagc
    19201 tgctgaattt gattatgcag ggactcaggc ctgtttggct ctaaaagaag aaggctataa
    19261 ggtcatactt gttaattcaa atcctgcaac gattatgaca gataaagaaa tcgctgataa
    19321 ggtatatatt gagcctttga ctcttgaatt tgttaatcgt atcattcgaa aagaaaggcc
    19381 agatgccata ctgccaacct taggtggtca aacgggcctc aatatggcaa tggcattatc
    19441 taaagctggt attcttgatg atttagagat tgaattgtta ggtactaagt tatctgcgat
    19501 tgatcaggca gaagacagag accttttcaa acaattaatg caagaactag accaacccat
    19561 tccagaatct acaattgtta aaacagttga tgaagctgtt acatttgcta gagatatcgg
    19621 ttatccagtc attgtgcgcc cagcttttac tcttggtgga actggtggag gaatatgtag
    19681 tagtgaagaa gaactttgtg aaattactga aaacggccta aagttatcac cggtcacaca
    19741 gtgtttgatt gaacgctcaa ttgctggctt caaagaaatt gaatacgaag taatgcgaga
    19801 tagtgctgat aacgcgctgg ttgtttgtaa tatggaaaac tttgatcccg tagggattca
    19861 cacaggagat tctattgtat ttgcaccaac acagaccctt tctgatattg aaaatcaaat
    19921 gttacgtgat gctagtttaa aaataattcg agctctgaaa attgaaggtg gctgtaacgt
    19981 ccagttagca ctagatcctt atagtttcaa atattatgtt attgaagtaa atcctagagt
    20041 gtctcgttcc tctgcccttg cttcaaaagc aacaggttat cccattgcca aattggctgc
    20101 aaagattgct gttggcctaa cacttgatga aatgattaac cctatcacag gcactactta
    20161 cgctatgttt gaacctaccc ttgattacgt tgttgctaaa attccgcgtt ttccgtttga
    20221 taaatttgaa catggtgaac gccaactggg aactcaaatg aaagcaacag gtgaagtcat
    20281 ggctattggc cgtaatttgg aagagtcctt actaaaagcc tgtcgttctt tagaaattgg
    20341 tgtttgccat aatgagatga caagtttaag taacattagt gatgaagagt tagtgactaa
    20401 agtgattaaa gctcaagatg atcgactttt ttacttgtca gaagcaatac gacgaggtta
    20461 tagtatcgaa gaacttgaga gcttgaccaa gattgattta tttttcttgg ataaattact
    20521 tcatattgtt gaaatagagc aagatctaca aatgcatgtt gagcatttgg aaagcctcaa
    20581 aaaagcaaag cgttatggat tttcagatca aaaaattgca gaaatttggc aaaaggatga
    20641 aagtgatatc agagcgatga gacattctca tagcctgtat cctgtctata aaatggtaga
    20701 tacttgtgca gctgaatttg atgctaaaac cccttatttt tattcaacat atgagttgga
    20761 aaatgaatct gttcagtcga ataaagaatc aattttagtt ttaggttcag gccctattcg
    20821 tattggtcaa ggggttgaat ttgactacgc aacggttcat tctgttaaag ccatccaaaa
    20881 agctggctat gaagccatta tcatgaattc aaatccggag actgtatcta cagatttttc
    20941 ggtatcagat aaattatatt ttgaaccgtt aacctttgaa gacgtcatga atgtgataga
    21001 tttagagcag cctaagggag taatagttca gtttggagga caaactgcta ttaacttagc
    21061 acaagcactt tctgaggcgg gtgttacaat attaggaaca caagtagagg atttggatag
    21121 ggctgaagac agagatttat ttgaaaaggc gttgaaagag cttggaatcc cacaaccaca
    21181 aggtcaaacg gcaactaatg aagaagaagc attagaggca gctaaaaaaa ttggttttcc
    21241 agtcttagtt agaccttctt acgtacttgg cggccgggct atggaaattg ttgaaaacaa
    21301 agaagatctt agagaatata taagaacagc ggttaaagca agtcctgaac atcctatctt
    21361 agtggactct tatattttcg gtaaggagtg tgaagtggat gccatttcag atggaaaatc
    21421 agtcttaatt cctggtatta tggagcacat cgaaagagca ggtgttcatt caggagattc
    21481 aatggcagtt tatccgccac agcaattatc taagcaaatt caagaaacaa tagctgagta
    21541 tactaaacgt ttagccattg ggttaaattg tattggtatg atgaatgttc aatttgttat
    21601 taaaaatgaa caagtctatg ttattgaagt gaatccacgc gctagtcgta ccgtgccttt
    21661 cttatcaaaa gtaacaggta ttccgatggc tcagattgct actaagttga ttttaggtca
    21721 aacattaaag gatctaggat atgaagatgg tctttatccc caaagtcaac tggtgcatat
    21781 caaagcgcct gtgttttcat ttacaaagtt ggcacaggtt gatagtcttt taggtcctga
    21841 aatgaaatca actggagaag taatgggttc ggatactagt cttgaaaaag cactttataa
    21901 agcgtttgaa gccaataatt ctcatttatc agaatttggt caaattgtct ttactatagc
    21961 agatgatagt aaggcagaag ctctaagtct agcaagacga tttaaggcga ttggctatca
    22021 aattatggct actcaaggta cagcagctta ctttgctgag caaggcttaa gcgcgtgttt
    22081 agttggaaaa attggtgatg ctgccaatga catcccgaca ctcgtgcgac atggtcacgt
    22141 tcaggcaatt gttaatacag ttggaattaa acgcacagct gataaagacg gacagatgat
    22201 tagaagttca gctattgaac aaggagttcc tttatttaca gctttagata ctgcaaaagc
    22261 tatgttaacg gttttagaaa gcagatgttt taacatcgaa gcgatataaa aattaaaaaa
    22321 tcatttttaa gaaaacttaa aaatgatttt tttagattat aatgacaaaa gtttcagtaa
    22381 gctattaatt tgtcatagca tataagtcat tatcttgtgg cttgttttcg actaagggtt
    22441 aagaatttct tgacaacaat ataaaaaggt catatacttg tcttatacaa atagtacaaa
    22501 aaggggagga tagaaatgtc taaaagagga aaaattaaaa taacaacgaa aacaaagctt
    22561 attacagcta gtgttataac gctagtatta attataactg gagtagtctt gtggaaacaa
    22621 caacaaaata cgctaacagc tgatatcgct aaagaacctt actctactgt tagtgtaact
    22681 gaagggagta ttgcttcttc gactttactc tcaggtactg taaaggcttt atcagaggaa
    22741 tatatttatt tcgatgctaa taaaggaaat gatgcaactg ttacagttaa agtaggtgat
    22801 caggtaacgc agggccagca attagttcaa tataatacaa caacagctca gtcagcttat
    22861 gatactgctg ttaggagtct taacaagatt ggccgacaaa ttaatcatct taaaacatac
    22921 ggagttcctg ctgttagtac agaaactaat aaagatgaag ctaccggtga agagacgaca
    22981 acaacagttc aaccatcagc tcagcaaaat gctaattata aacagcagct gcaagattta
    23041 aatgatgctt atgcagatgc acaagcagaa gtaaataaag cgcagatagc gttaaatgat
    23101 acagtagtta tcagtagtgt ctctggaact gttgtggaag taaataatga tattgatcct
    23161 tcttcaaaga acagtcaaac acttgttcac gtagcaaccg aaggacagct tcaggtgaaa
    23221 ggaacattga cagagtatga tttagcaaac gttaaggttg gtcaatctgt aaaaattaag
    23281 tctaaagttt attctaatca agaatggact ggaaaaatat catatgtttc aaactatcca
    23341 actgagtcta atgcaggttc aacaacgcca gcaggtagca ctggagcggg aagttctaca
    23401 ggagctgcct atgattacaa gattgatatt ataagtcctc tcaaccagct taaacaaggt
    23461 ttcactgttt ctgttgaggt tgttaatgaa gctaaacagg ccttagttcc tttaacggct
    23521 gttattaaga aagataaaaa acactatgtt tggacttatg atgatgctac tggcaaagcc
    23581 aaaaaagtag aggtgacact tggaaacgca gatgcacaac aacaagaaat tcataaagga
    23641 gtagctgttg gtgacattgt tattgccaat ccagataaaa atatcaaacc ggataaaaaa
    23701 ctagaagggg ttatttcaat aggtaccaac acaaaaccgg aaaaagattc tcaatcaaag
    23761 aataaaaaat caggggtgga taaatagtgt cgcatgaaaa aaaacaatta atgcaattat
    23821 caaatattgt gaagagctat caaaatgggg atcaagttct taaagttttg aaaggtatta
    23881 atttaacagt atatgagggt gaatttttag ctattatggg tccgtctggc tcgggaaaat
    23941 caacactaat gaatattatt ggtttattag atcggccaac atccggagat tacacattac
    24001 ataatactaa aatagaaatc cttaatgatc gtgaattagc aaaagtacga aacgatgaaa
    24061 tcggctttgt ctttcaacaa ttctttctgt tagctaaatt aactgcatta caaaatgttg
    24121 agttgcctct tatttatgct ggtgtaaacg tctcaaaaag acgtgaacag gctaagcaat
    24181 ttcttgaaaa agtaggactt ggacgacgta tcaaacatct accgtcagaa ctgtcaggag
    24241 gtcaaaagca aagggtggct atcgcacgag ctttagtaaa tgacccatct attattttag
    24301 cggatgaacc aacaggtgca ctagatacta aaacaggtca gcaaattatg gaactcttga
    24361 cagagttaaa caaagaaggt aaaacaatta ttatggttac tcatgaacca gaaattgctg
    24421 attttgcaac tcggaaaatt attattcgtg atggtgatat cacaacggat actacagcta
    24481 gtgttgttat tgactaggag gaaaaataat ggaaaattgg aaattcgctc ttagctcaat
    24541 ttgggggcat aagatgcgct caatcctgac aatgttggga attatcatcg gtgtagcagc
    24601 agttgtgatt attatggggt tgggaaatgc tatgaaaaat agtgtaacaa gcacattttc
    24661 tagcaagcaa aaagatattc aactctattt tcaagaaaaa ggagaagaag aagacctgta
    24721 tgccggattg cacacgcatg aaaataatca cgaagtgaag ccagagtggt tagaacaaat
    24781 tgttaaagat atcgatggta ttgattctta ttactttacc aattctgcta cttctactat
    24841 ttcgtatgaa aaaaagaaag tagacaatgc ttcgatcata ggggtaagta aagattattt
    24901 taacataaaa aactatgata tcgtagctgg tcgtacacta acagataatg attattctaa
    24961 tttttcgcgt atcattcttt tagatacagt attagccgat gacctatttg gcgaaggtaa
    25021 ttataagtca gctttaaata aagtggtaag tttatctgat aaagattatt tagttattgg
    25081 tgtttataaa actgatcaga cgccagtttc ttttgatgga ttatcaggcg gcgctgtcat
    25141 ggcaaatact caggtagctt cagagtttgg caccaaagaa ataggatcta tctacattca
    25201 tgtgaacgat attcaaaaca gtatgaactt aggaaatcaa gcagctgata tgctaaccaa
    25261 catatcgcat attaaagatg gacagtacgc tgttcctgat aatagcaaaa tcgttgaaga
    25321 aatcaatagt cagttcagta tcatgacaac agtcataggt tcaattgcag cgatctctct
    25381 tttagtggga ggaattggtg ttatgaatat tatgttggta tctgtcacag agcgaactag
    25441 agaaattggt ttacgtaaag cacttggtgc aacacgcctg aaaattttat cacaattttt
    25501 gattgaatcc gttgttctaa cagttcttgg aggcttgata ggattattat tagctcaatt
    25561 aagtgtaggg gctttaggaa atgcgatgac attaaaagga gcttgtattt ctcttgatgt
    25621 ggccttaata gcagttttat tttctgcctc aataggtgtc ttttttggaa tgttgccagc
    25681 taataaagct agtaagttag atccaattga ggcattacgt tatgaataga atagttcgta
    25741 tcaattaatc tatagacgct gactagttta acgtctgatt atatctagtt tttgtattag
    25801 cacagtagtt atatcattga agtgttagtg cattttttga ggtgataata tctagcaaag
    25861 ctatcattgg tatgatgatg tggatagcgt ataagtgata catctcttat ttctagctga
    25921 acttcggttc ggctaggaag ttgtcaggca atgagttgtt taaaataaag gagattagtt
    25981 caatgacatt tttatctgat ttgatatcat taatgacaaa aatcagatta tcttgggtaa
    26041 taaaggcggg tatttttcaa ttattatttg taacgattgc taatattgtc ttatcagaa
//
LOCUS       DATUYR010000022        24971 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.022, whole genome shotgun sequence.
ACCESSION   DATUYR010000022 DATUYR010000000
VERSION     DATUYR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24971
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.022"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..173)
                     /locus_tag="VCI33_000835"
     CDS             complement(<1..173)
                     /locus_tag="VCI33_000835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831770.1"
                     /translation="MKDNKELMKDKKVATNRRSIIFISVPKSSIITTNLLEPEVGINE
                     KLAKNIAVTIAEN"
     gene            complement(468..845)
                     /locus_tag="VCI33_000836"
     CDS             complement(468..845)
                     /locus_tag="VCI33_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEP2831771.1"
                     /translation="MLIYPATFTQDTDYIMVTFPDVPEAITQGEDFQEAYEMAVEVLG
                     FALEDYTDYPKASAVSDLKEQYPDSDIALIGIDMVAYMKKYHSKKVRKNVTIPEWLND
                     AAEDKNLNFSQVLTEALELKLQA"
     gene            complement(897..1082)
                     /locus_tag="VCI33_000837"
     CDS             complement(897..1082)
                     /locus_tag="VCI33_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEP2831772.1"
                     /translation="MPMTPKQMIKLLKKNGFYEVSQNGSHKKFRDNNGHQTIVPMHNK
                     DLGKGLEDTILKQAGLK"
     gene            1145..1612
                     /locus_tag="VCI33_000838"
     CDS             1145..1612
                     /locus_tag="VCI33_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEP2831773.1"
                     /translation="MRIKYTKGGDGKLSKLTLKQKRFADEYIISGNAMESAIKAGYSH
                     NYSKAQSHKLLENVGIKEYLDERFEKMDSKTIADQKEVLQYLSSVMRGEQQEKTLISI
                     GELGQEIVDIDVGAKDRLKAAELLGKRYRLFTDKIEAEVQGTVVFVNEDDIPD"
     gene            1590..2897
                     /locus_tag="VCI33_000839"
     CDS             1590..2897
                     /locus_tag="VCI33_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEP2831774.1"
                     /translation="MKTTYQTKPKLEIKVDLPKTIGIGYGAFWRSKNFYRVVKGSRGS
                     KKSKTTALNFIVRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNQLKVTHLFKFNES
                     LPEITVKATGQKILFRGLDDELKITSITVDVGLLCWAWFEEAYQIETEDKFSTVVESI
                     RGSLDAPDFFKQITVTFNPWSERHWLKRVFFDEETKRADTFSGTTTFRVNEWLDDVDK
                     RRYEDLYKTNPRRARIVCDGEWGVAEGLVFDNFEVVDFDVEKTIQRVKETSAGMDFGF
                     TQDPTTLICVAVDLVNKELWLYNEHYQKAMLTDHIVKMIRDKNMHKSYIAADSAEKRL
                     IAEIKSKGVSGIVASLKGKGSIMQGVQFMQGFKIYIHPSCEHTIEEFNTYTFKQDKEG
                     NWLNEPIDKNNHVIDAIRYALEKYHIRSNESNQFEVLRAGFGY"
     gene            2909..4411
                     /locus_tag="VCI33_000840"
     CDS             2909..4411
                     /locus_tag="VCI33_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP2831775.1"
                     /translation="MYTESFRDSTGKTKTLEFRFHREARMRYQAESLESLLTEKYKLL
                     REMIEHHDKVQKPRIQELLDYAEGNNHTISEIGRRKDDDMADVRAVHNYGKYISTLKQ
                     GYLVGNPIRVEYIDGTEQQQDLLKDLSVKNNFHQLNRRLVKDLSKVGRAFELIYRSMD
                     DKTEVVRLDPREVFVIYQNNLEQSSLAGVRYYNKNQLDGTTKIVELYTDNKILKFEYD
                     GDLTLIREASSHAFGSVPITEYLNTDDGMGDYETELSLIDLYDAAQSDTANYMQDLSD
                     AILAIFGRVSFPHDVQTAEQRIEFVKVMRKARLLNLEPPVDQDGREGSVDAKYLYKQY
                     DVQGTEAYKNRIVSDIHKFTNTPDMTDSKFAGQQSGEALKWKVFGLDQERVDMQALFE
                     QSLKRRYKLIARVSQLLKEIDDFDISKLKITFTPNLPKSLQEKIEAFKALGGELSQET
                     AMAITDIVEDAKKEISLINSESKSRSQLAQKLEETSRLTDRELAHDHQKE"
     gene            4392..5954
                     /locus_tag="VCI33_000841"
     CDS             4392..5954
                     /locus_tag="VCI33_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEP2831776.1"
                     /translation="MTTRKSKYWRDRIKKEMDAKEADDISLEQSMKQLHDYHFRNIEK
                     EIESFYQRYADKEKIDLSEARKRASELDISAYQKKAKELIAKAEKLRKEGRTVTKADF
                     THQENADMAIYNLAMKTNALELLRLNIDLEMQELANGEHKLTKKFLDEGYRKEVEFQA
                     GLLGLSVASQANVKNLADAVINANFKGAKWSDNIWDRQDKLRSIISQSVQSAILKGKN
                     GLTIARDIRREFDVSASYAKRLAITEHARVQMEVGRLSMAENGFAMFDILPEPKACDV
                     CKDIAKHGPYHLDKWRIGENSPPFHPYCRCAVVGLDKKQEEKVNDKSEKFENITPNLM
                     PLFSKFASKITDLQRKIVYSADLADTGYIRTPHAFDINNTLRNKGYNYLNVDDKLITD
                     TLDSVISINSTPKNIKVYRFDDFELLGSINEQNNNIFDSGNFMDKLNQGGLSYTNDGY
                     TSASYDVKKNVMGYRPIKTEIKVPKGSHVYLTDNEEESEIILPRGTKYDIINAKINEY
                     EEIEITMEIRKE"
     gene            5958..6143
                     /locus_tag="VCI33_000842"
     CDS             5958..6143
                     /locus_tag="VCI33_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831777.1"
                     /translation="MDFSDFLNKKQKEWDESHPIPDFSAMSDEELLYQPMSEALVSER
                     FAKELSKEVRKRNLLSK"
     gene            6213..6527
                     /locus_tag="VCI33_000843"
     CDS             6213..6527
                     /locus_tag="VCI33_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831778.1"
                     /translation="MNRESKPGMESVKIGGLTYSVEKASDLQGKDGNWGLIHYKTQQI
                     KLDDSLTEQLEDQTLIHEIVHGILVEAGYTNHEEDQANRIGLILYQVLQDNDFSWLYK
                     GE"
     gene            6530..6796
                     /locus_tag="VCI33_000844"
     CDS             6530..6796
                     /locus_tag="VCI33_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831779.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAIQAKALRNQNKQIAELRAIIQ
                     QNVQAINREFVTAKNTILDNQLAIKLIGDDVGHIKQNYKRKWRK"
     gene            6940..7473
                     /locus_tag="VCI33_000845"
     CDS             6940..7473
                     /locus_tag="VCI33_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEP2831780.1"
                     /translation="MAEETQVVEMVEEQVVPEAKQPQDEKKYTDADVDAIIDKKFAKW
                     KSEQEAEKSEAKKMAKMNEKEKADYEKQKLLDELQELKNDKTRNELTAVARQMFAESE
                     INVNDDVLGLVVTLDAEQTKANVTTLANAFAKVIADDRKALVRQTTPSTGGGLRKQTN
                     YGANLASKAAQQSTKLF"
     gene            7483..7863
                     /locus_tag="VCI33_000846"
     CDS             7483..7863
                     /locus_tag="VCI33_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEP2831781.1"
                     /translation="MNKRKVTTSKEILHNLDYEAISVTLDSKKIGKEVVPAGTVLAGV
                     SGSVFEDRKQKVKAVDNSAVESEDNICGILLTDVDLTNGDAVGSCIYRGTINADKLAD
                     SSIAGKYEVLEEELPHIVFIKGGK"
     gene            7866..8948
                     /locus_tag="VCI33_000847"
     CDS             7866..8948
                     /locus_tag="VCI33_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEP2831782.1"
                     /translation="MALIHEIITSENIKGFYNAKNENVGNTLGENAFPPKQQLGLKLS
                     FIKGAAGKPVTLKAAAFDTKVPLRDRMTVELLDEEMPFFKEAMVVKEADRQQLSLLAQ
                     TKNQELIDTALAAIYNDKVTLIAGAKARLEAMRMEVLSKGKIHVASNGVMKDFDYGLD
                     SSQTTKPDTKWEQVDTATPLKDIEKAIEVMAERGFVPEAIIMNSKTLSLIKNASNTLD
                     VVKPMAPDGAAVTKGDLETYIADELGLKILLKDGMFVGDDGKAKKYFPDGFATLVPNG
                     NLGYTVFGTTPEQSDLLGGEATDAEVSIVETGIAITTTKTTDPVNVQTKVSMIALPSF
                     ERLEEVQIINATESEEETKKENSFEM"
     gene            8958..9200
                     /locus_tag="VCI33_000848"
     CDS             8958..9200
                     /locus_tag="VCI33_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831783.1"
                     /translation="MPRVIRAFKDKVTKVVYEVGDIYSGDRVEFLTEGGVLEPSVDFD
                     KLKVSEIKSKLDELSIEYDAKLKKSELLKLLKQTIG"
     gene            9214..9567
                     /locus_tag="VCI33_000849"
     CDS             9214..9567
                     /locus_tag="VCI33_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEP2831784.1"
                     /translation="MDAVNTSSVISNVKLDLGILDSQQDDLLNMLLKRVTDHFKAKYG
                     AVEIDSAFSFVLEDCLIARFNRRGAERAKSESVEGHTTTYYDFLDEFEPYDAMIMAKL
                     NLIKDKSRKGGLYFL"
     gene            9564..9872
                     /locus_tag="VCI33_000850"
     CDS             9564..9872
                     /locus_tag="VCI33_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831785.1"
                     /translation="MRYADRVTFVKTTDEQYNPDLGEYTHTEVISITKPCFVMDMGME
                     KSVQIFGDYQKDRKVIYLKQPYTKAFDYCEYEGKRYKAQANKLGAIVFYLEGDDSIGG
                     "
     gene            9853..10218
                     /locus_tag="VCI33_000851"
     CDS             9853..10218
                     /locus_tag="VCI33_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEP2831786.1"
                     /translation="MILLVADISLKVVGTAGLKKKLELIVKKDAVKKIVRDNGTQLQR
                     KMINKAVFTKGYSTGATRRSITMQIGDGGLSVKVKPGTHYAGYLERGTRLMSKQPFVL
                     PALKEQKVKFRKDLEALVK"
     gene            10215..10604
                     /locus_tag="VCI33_000852"
     CDS             10215..10604
                     /locus_tag="VCI33_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEP2831787.1"
                     /translation="MIKTRDQSIFDEMFKRIQSLGFKVYDYKPMTEVPYPFVEMESTD
                     AEYIPNKDDIKGSVELMLSVWGVQKKRKQVSDMASAIFSQAMSVARTDGFCWSFNIRQ
                     SSIQMLDDTTTVTPLKRAIVTLRFNLR"
     gene            10659..11282
                     /locus_tag="VCI33_000853"
     CDS             10659..11282
                     /locus_tag="VCI33_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEP2831788.1"
                     /translation="MSKAQEQTKQLEAKQGIHSILLFRLLKEASSEVATKLAFQTEHE
                     VGKSRDVDGQKTKDGIIQSVGALEYDFKATSILAKGDELAAKLEKAMEDGELVEIWDI
                     DSEEISKNGDSDNKLAKVWGINKNGTNGGNGKCLATYYQGYISSFSAKKNAEENIVIE
                     MEFAINGVGKKGFATLTDAQKAAVQYAFKDTTKSNSEETKKENSFEM"
     gene            11336..11689
                     /locus_tag="VCI33_000854"
     CDS             11336..11689
                     /locus_tag="VCI33_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HEP2831789.1"
                     /translation="MQLEIKGKTHNVKFGTRFVAEMDKNHIAERQGFKFGAGLQSSVP
                     FLIDHSVVTLAEVIYTGTITEPPRPSLNDIYDYIDEVEDIEKLFDDVLDELRQSNASK
                     LFMAQVEKDLAEAKA"
     gene            11764..12060
                     /locus_tag="VCI33_000855"
     CDS             11764..12060
                     /locus_tag="VCI33_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831790.1"
                     /translation="MTDINEIGRLTLYEYDLLMTGKALAAVDESHKAHKQAWINHQVT
                     ATKLVGGKKNKKEVPVYKKFKDFFDYEEEIRKITQEIDEGYDKKGMDLLLKANL"
     gene            12075..15710
                     /locus_tag="VCI33_000856"
     CDS             12075..15710
                     /locus_tag="VCI33_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEP2831791.1"
                     /translation="MGESYSVEAVLTAVDKTFGKTLQSAIRSIEGLEKRSTGFSSVSQ
                     KASSMFKSMLGANLAGQAISAMTRTVSSGLGSMLGEMNSSAKAWKTFDANLADIGFGK
                     KQILAAKTAMQDYATKTIYSASDMASTYAQLAAVGVKDTGKLVKAFGGLAASAENPKQ
                     AMKSISQQMTQAVGRPTVAWQDFRIMLEQAPAGMAKVAKSMGKNLDELVADIQAGRVK
                     TSDFLEAVKKAGNDKSFQKMATEFKTVDQAIDGMREGLSNKLQPAFEKVNQFGIRAIE
                     AIGKQLDKVDFSKFASNLGKFLEGINIDKIVSNISSAISSVTSKVKEFWDGFKQTGAI
                     SAFSGALQSVWEALKNVASAMSGGNWKTFGATVGGTVKHVSNFAKAVSDVLGKMDPGR
                     LRSWIATFAAVAGGFKLFEKLTGQSVIGSFLDKIGSKFGLFGNKAKEGTDKASNGARR
                     SGGIISQIFSGLGNIVKSAGTAISTAAKGIGAGIKTALSGIPPIISSLGTAISTVAQG
                     IGTGLAIAFKGLGSAIAMVPPTTWLALGAAILMVGAAFALAGTQADGISQILRTVGDV
                     VVQILQQVTDSLATLLPIIANAIGSMLPIVAGAISQIVGAVAGGLSQLVIAVSTGASL
                     VIGAFTGLLGGISGVINSISAVIQSLTGVITAVFNGIATVISSVGSAIKDVLTGLGTA
                     FEGFGNGVKSALEGVGAVIESFGSAVRNVLDGVANILDSMGTAALNAGRGVKEMAKGI
                     KMLVDLSLGDLVATLAAVASGLGKMASSAGEMTTLGSAMSKVANGMTRLATSATIAIT
                     GLTVFATTMATIKTAVATLPPVLTMAASGFTTFTTQAVAAVTGLAAINAPITMFKAQL
                     MTITPALAQAGAGFAAFVAQSSTFSTGLASAGPTIAAFNANLMSLSATTGALVASIAG
                     LSAVLSVVSAGFSQIGASATATVGQIQAFASSTTVVSSAFASMQSMIQSAMAAIVSSI
                     ITSFNQAASQMQSILSRMLSQARTFGSQLEQQMRQSGQRSGQNLARGLSSQQSAVINA
                     ISSMVNAAVSRANAGAGPMRQAGAYIGQGLAQGMYSALGAVTAAANALVAQAERAARA
                     KAMIHSPSRLFAKRVGQYIPQGVAMGIDKNADVVDDSVGGLFDSINSFDFNIADRLAS
                     IGAKFQGVIKSESSQSLSQQQEFVHTAQPAYINFSLGGNEYEAFVSDITNQQAKIEKI
                     RLKRSSW"
     gene            15742..16521
                     /locus_tag="VCI33_000857"
     CDS             15742..16521
                     /locus_tag="VCI33_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEP2831792.1"
                     /translation="MYEFNDTIRGTPKVTFNLKTTIGEKVLEEELNNDLGTFRTLTVS
                     GRDVVDLEHQTTSVLGRNGEYFHNATVEVRKLEIKAKITGKDNQSMRLQYEKLNRLIV
                     SHNQVFLSFSDEPDRNYLGIFKSKDVPEEVSNEQIIGLTFICYNPFKMSDVKTKKGTS
                     IQNGGLFQTKPIITLNLSSPTKEIKLLHVESQKYIRLTGTYTTDEIKIDMATGKITQN
                     GRNILGDLDMINSRYFELLPGNNTLQCANAAITAEFREVYL"
     gene            16518..18572
                     /locus_tag="VCI33_000858"
     CDS             16518..18572
                     /locus_tag="VCI33_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP2831793.1"
                     /translation="MIYLFDKLERLVATVGTDDLLSWHFKVKNNDWDQASFEVPVDYD
                     VEPFVYFGFFNYDPNQKEDVFKLFKVIDYNLEDSKFYKGLDKAESDLDTIAIIKDKRF
                     RQSSADACIDGALEGTGYQVGKVEGITNVRTLSYYYISPRAALIKIVEAFNCEFNVRY
                     TFINNKITSRYIDLKKRFGKPTGKQFEHGNNLLKVVYEESTDDIVTCLIGRGKGEEIQ
                     HEEAEPKDVEGHLPQEERRQGYGRRIEFTDVVWSVEKGDPIDKPAGQNFVALDSAREE
                     YGLSQNGELKHRWGVFVNEEIEDKTELLKATWEELQRLSIPIRIYKAEILDIGPETWK
                     GDSVAIIYDEVKIAFETRVDEIDIDKLNFNRSVVTLGDYSVVQNRESRSRKEAVQNMI
                     DESLETITDLGMTFQEFLQGIEKRIETGKKEMEDNWRKVNLEFDNFKKKVEQEGLQFN
                     TLKEQIKEVDERTDKELEEFRATLKNLALPEEAIKKITEAIKVDDIPSLKQNFDDLKN
                     KVSETSETARLNAEIIGTDGKTRYNKNLLVGDPNRVKTYDQDYIEVEANDGGFKRGET
                     YTISFSQTCELLKKVAITLTQINNKGVKLVLTPTKAKMDAQTFEVTKDKQSIEVYPLS
                     YTGVLTGDWYKSKSVEINASEAQELALEMAYKEIADAKGATIIGKQSDKPKIILDGRR
                     DR"
     gene            18569..19783
                     /locus_tag="VCI33_000859"
     CDS             18569..19783
                     /locus_tag="VCI33_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831794.1"
                     /translation="MTSVETIPIKIVFDRKDASEWQSTNPVIDEGELVVELDTHKLKV
                     GDGKTSYNDLPYYEGPQGESITKVQLSENGDLSVWIGDKETKLGNIKGQKGDKGTSIT
                     DITKDGETLTIKLSDDTQKIFNIPNGQKGDRGKGVESARIDESGHLKLKIEEESELDL
                     GNVKGESGPKGDSITITNHKRVSDGTQVSFSDGTQIVVPKGDTGDVNGINLEDYVKKS
                     ELKNVGSADVKAINDFLGLSQKVFTSSYSYTDSLLKSYAKPSYSASWYVNESTVSTKN
                     GDKVLITIHNTTTQADNYLEVAVTYVGANYVTATSTGRLLTTPGEVKVVTKKQAEKDY
                     AAKKHKHEISDIAGLNERLSGYLRQADIQSQLNNIGKLKDTQTGQYLEVKVVDKGQVP
                     SNTSGMIVFERS"
     gene            19770..20099
                     /locus_tag="VCI33_000860"
     CDS             19770..20099
                     /locus_tag="VCI33_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831795.1"
                     /translation="MRGHNLLKDLNNIIINNKTIDRIICNNTIVYQRSWDLLFDGLLT
                     SAGKKLDIYPFYKIVINNAFFVKTPTTKNNTIFLAGHKILALQDSHFCFFRALEKSFF
                     VKIYGKN"
     gene            20110..22005
                     /locus_tag="VCI33_000861"
     CDS             20110..22005
                     /locus_tag="VCI33_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEP2831796.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFTADGDTKSVKIANNKCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGTGNPTGLFKDLKEISLELTDTKNSKLWSKIKL
                     NNQGMIEEYYNGTIKSEIIKTAEGTQQRISSETDKKLALINETVSGIRREYQDADRQL
                     SSSYQAGIEGLKATMANDKIGLQAEIQTTAQGLYQRYDNEIRKLSAKITTTSSGTTEA
                     YESKLDGLRAEFTHSNQGMRVELESKISGLQSTQQATARQISQEISNREGAVSRVQQG
                     LDSYQRRLQNAEGSYSSLQQTVSGLQSDVNSPNSKLNSRISQLASQIDQRVTRADVTS
                     IINQSGDSIKLAIQRAGGIDAKMSAKEIVSAINLNGYGVRISGERIALDGNTTVNGAF
                     GAKIGEFIKLKADQIIGGTIDANKINVINLNASSIVGLDANFIKARIEYAITSLLEGK
                     VIKARNGAMTIDLQSGQINHYTNESAMRRIDSSTASQFIKMTKSGFISEIGNMQAAMT
                     VIGSNSDGSENHENKTFGGIRIWNGKSSYQSTSFVELVGNRVAIYGNKNRSPWLFDST
                     TSGYAYLIPQNESGIKHAIGRADRKIDQIHVGDIYVQGERVAMMLKDLANRIGYIGTG
                     GWANRIG"
     gene            22019..22180
                     /locus_tag="VCI33_000862"
     CDS             22019..22180
                     /locus_tag="VCI33_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831797.1"
                     /translation="MQEKLLGKIINDLTLKVANLTLENAQLKAQHEIELEELNAQLDE
                     ATAPKEEGK"
     gene            22183..22800
                     /locus_tag="VCI33_000863"
     CDS             22183..22800
                     /locus_tag="VCI33_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831798.1"
                     /translation="MRNWKVTGNYPQWDGTGAVIDTQVIITDDRGAVIAEKIKKDLRT
                     ANDAEIIEAALEEFKKTAYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQKMINLQTIHMLTSGGKIDPDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEPFTYEAQTLKDLESEDKVTVIKYADLVKQD"
     gene            22811..23107
                     /locus_tag="VCI33_000864"
     CDS             22811..23107
                     /locus_tag="VCI33_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831799.1"
                     /translation="MIDFVQIGAFCGAALSILGVWGFIVNPFKKAMEANEFAMAQLKD
                     SIKELAYELKNLDREREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            23104..23289
                     /locus_tag="VCI33_000865"
     CDS             23104..23289
                     /locus_tag="VCI33_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831800.1"
                     /translation="MNNWFKKVAIKTIKTMAQTAVGLIGSSMLITDINWPTMLSAVLL
                     SGLTCILMNVSQIKEEE"
     gene            23401..24735
                     /locus_tag="VCI33_000866"
     CDS             23401..24735
                     /locus_tag="VCI33_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEP2831801.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSLGAQCWDGYADYCKYLGLPYANC
                     TNTGYARDIWEQRHKNGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSG
                     YGWFLGQNQGGANGAYNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNL
                     AQREADLFLSNLPTKKVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSY
                     KPFTLNNIDYQQIIAKYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYV
                     ESKPNASSADKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
     gene            complement(24811..>24971)
                     /locus_tag="VCI33_000867"
     CDS             complement(24811..>24971)
                     /locus_tag="VCI33_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2831802.1"
                     /translation="RIEIGTKDGKHEQIDLFDSPNEGTRSDIFAKYKDNRIINMKNFS
                     HFDIYLEK"
BASE COUNT         8454 a         4321 c         5530 g         6666 t
ORIGIN      
        1 tcattttcag caattgtaac cgcaatattc tttgctagtt tttcattgat accaacttcc
       61 ggttctaata aattagtagt gatgatggaa gatttaggga cagagataaa aataatggac
      121 cgcctattag ttgctacttt tttatctttc attagttctt tattatcttt catttctcta
      181 ttctttagta agactgatac tgatttaatt tcaattttag tagtttcaag ttggttgagc
      241 tcaacagtaa tgatgttcct aagttcgttc tttgtgctaa gaacattaat tttgttagtt
      301 gaaacttata ataatttcaa aaataaatag gtcatcacat tgtggtgact tttttgttat
      361 ggtccacgac atggttactt attagttaca aacgttgaaa ttacaatgtt tcgataggtc
      421 aacgtcgtgg ttttttatta tgtaaaaaag aaccacaaca gtggctctta tgcttgtaat
      481 tttaattcaa gcgcttcagt aagaacttga gaaaagttga ggtttttatc ttctgctgcg
      541 tcgtttaacc attcagggat agtcacgttt ttgcggactt ttttggagtg atattttttc
      601 atataggcaa ccatatcgat gccaattaaa gcaatgtcgg aatcaggata ctgttctttt
      661 aaatcagaaa cggcgctagc ctttggatag tcagtatagt cttcaagggc aaaacctaag
      721 acttcgacag ccatttcata agcctcttga aagtcttcac cttgagtaat tgcttcaggt
      781 acatctggaa atgtaaccat gatatagtct gtgtcttgtg taaatgtagc tggataaatt
      841 aacataatga tcctcctttg attactgtga gataagcaca ccatctggca agcggattat
      901 tttaaacccg cctgctttag aatggtatct tcaagacctt ttccgaggtc tttattgtgc
      961 attggaacaa tggtttgatg tccgttattg tctcggaatt ttttgtgact tccgttttga
     1021 ctgacttcgt agaacccatt cttttttagc agttttatca tctgctttgg ggtcattggc
     1081 atattgctta cctcactttc tatacttata ttatacacat aaacaatgta gatgtcaatg
     1141 caaaatacgc ataaaataca caaaaggcgg tgatggaaaa ttgagtaaat taacactaaa
     1201 acagaagcgg tttgcagatg aatacatcat ctcagggaat gctatggagt cagctataaa
     1261 ggctgggtat agccataatt attccaaagc acagtcgcac aagctattgg aaaatgttgg
     1321 aattaaagag tatcttgatg aacgatttga aaaaatggat tccaagacaa tagcagacca
     1381 aaaagaggta ttgcaatatc tttcttcggt catgagaggt gagcagcaag aaaaaacact
     1441 catcagcatt ggagagcttg ggcaagaaat agttgatatt gatgtaggag caaaagacag
     1501 gttgaaagca gctgaattgc ttggtaagcg gtacagattg tttaccgata agatagaggc
     1561 tgaggtgcaa ggaacggtgg tgtttgtcaa tgaagacgac ataccagact aagccaaagc
     1621 tagaaatcaa agttgattta cctaaaacta tcggtatagg ttatggcgct ttttggcgct
     1681 ctaaaaattt ttatcgagta gttaaaggca gccgtggatc taaaaaatct aaaacgactg
     1741 ctttaaattt tatcgtcaga ctgctgaagt acccttgggc taacttattg gtcatccgta
     1801 gatactcaaa cactaacaaa caatctactt ataccgattt taaatgggcg tgtaatcaat
     1861 taaaggttac acaccttttt aagtttaatg agagcttgcc agagataacc gtcaaggcaa
     1921 caggacaaaa aatcttattc cgtggtcttg atgatgaact caagataaca tctattactg
     1981 ttgatgtcgg tttgctctgt tgggcatggt tcgaggaggc ttatcaaatt gagaccgaag
     2041 ataagttttc aacagttgtc gaatcaatcc gcggtagttt agatgctcct gattttttta
     2101 aacaaataac agtcacgttc aacccgtggt cagaaagaca ttggcttaag cgtgtctttt
     2161 ttgacgaaga aactaaacgg gccgatacat tttctgggac tacaacattt agagtaaacg
     2221 aatggcttga tgatgtcgat aaaagacgct acgaagattt gtacaagact aatccaaggc
     2281 gggctagaat cgtctgtgat ggtgaatggg gcgttgctga aggtcttgtt tttgataact
     2341 ttgaagtcgt agattttgat gttgaaaaaa caattcaacg tgtcaaagag acttcagctg
     2401 gtatggactt tggttttact caagacccta caactcttat atgtgttgca gttgacctcg
     2461 taaacaaaga attatggctt tacaatgaac attatcaaaa agctatgttg acggatcata
     2521 ttgtcaaaat gataagagat aaaaatatgc ataaatctta tatcgcagct gatagcgctg
     2581 aaaaacgtct aatagcagag ataaaaagca aaggtgtatc tggtattgtt gccagtctta
     2641 agggtaaagg gtctattatg caaggagttc agtttatgca aggttttaaa atatacatcc
     2701 acccttcttg tgaacacaca atagaagagt ttaatactta cacttttaag caagacaaag
     2761 aaggtaattg gttaaacgaa ccgatagata agaataacca cgttattgat gcgattagat
     2821 atgcgcttga aaaataccat atcagaagca acgagtcaaa tcagtttgaa gttcttaggg
     2881 ctggttttgg ttactagaaa ggaaaataat gtacacagaa tcatttagag atagtacggg
     2941 aaagactaaa acattagagt ttaggttcca ccgtgaagct cgcatgaggt atcaagcgga
     3001 aagtctagaa agcttgttaa ccgaaaaata taagctactc cgtgaaatga ttgaacacca
     3061 cgataaagtc caaaaaccac gcatacaaga gcttctagat tatgcagagg gaaataacca
     3121 caccatcagc gaaataggcc gtaggaaaga cgatgacatg gctgatgttc gtgctgtgca
     3181 taactatggt aagtatattt caacgctcaa acagggctat ttggtgggta atcctattcg
     3241 tgtagagtat attgatggta ccgagcagca gcaagaccta ttaaaggacc tatctgttaa
     3301 aaacaatttc caccagctga accgcagatt agtaaaagac ctatccaagg ttggtcgagc
     3361 gtttgaattg atttatcgca gcatggatga caagacagag gtcgttagac tagatccacg
     3421 ggaagtattt gttatctatc aaaataacct agagcaatca agcttagctg gtgtgcggta
     3481 ctataacaaa aatcaattag atggtactac aaaaattgtc gagctttaca ccgataataa
     3541 aatcctgaag tttgaatatg atggtgattt aacactgatt agggaggctt cgtctcatgc
     3601 gtttggttcg gtaccaatca cggagtatct caacacagat gacggcatgg gcgactacga
     3661 gacagagttg tctttaatcg acttgtatga tgcagctcag tctgatacag ctaactacat
     3721 gcaagatttg tcagacgcaa ttctagccat tttcggacgt gtgtcatttc cgcatgatgt
     3781 tcagacagcc gaacagcgta tcgaatttgt gaaagtgatg cgtaaagcta gattgcttaa
     3841 cctcgagcct cctgtcgacc aagacgggcg tgagggatct gtagatgcca aatatctata
     3901 taaacaatat gacgtacaag gaaccgaagc ctataaaaat cgtattgtgt ccgacatcca
     3961 taaatttacc aatacgccag acatgacaga cagtaagttt gccggtcaac aatccggaga
     4021 ggcgttgaag tggaaagtgt ttggtcttga tcaggagcgt gtcgacatgc aagctttatt
     4081 tgagcaatct cttaaacgta ggtacaaact aatcgctcgt gtaagccaac tgcttaaaga
     4141 gattgatgac tttgacatca gcaagcttaa aatcacattt acgccaaacc tacctaagtc
     4201 gctacaagaa aagattgaag cctttaaagc attgggtgga gagttgtcgc aagagacagc
     4261 tatggctatt acagacatcg tggaagatgc taagaaagaa attagcctta tcaatagcga
     4321 gtcgaaatca cgtagtcaac tagcgcagaa gttagaagaa accagtagat tgactgatag
     4381 ggagttagct catgaccacc agaaagagta aatactggcg tgaccgtatc aagaaagaaa
     4441 tggatgctaa agaggcagac gatatctctc ttgagcaatc catgaagcaa ttgcacgatt
     4501 atcatttcag gaatatcgaa aaagaaattg agtcgtttta tcaacgttat gctgacaaag
     4561 agaaaataga cctttcagaa gcccgtaaga gagcttctga gcttgatatt tctgcttacc
     4621 agaagaaagc taaggaactt attgcaaagg ctgagaagct acgaaaagag ggtagaacgg
     4681 taacaaaagc cgacttcact catcaagaaa atgcagacat ggctatttac aacttagcca
     4741 tgaaaaccaa cgctttagaa ttgttgcgct tgaatatcga cttggagatg caagaacttg
     4801 ccaacggcga acacaagcta accaagaaat ttcttgacga aggttaccgt aaagaggttg
     4861 agttccaagc tgggctatta ggattatcag ttgctagcca agcgaatgtg aaaaacttag
     4921 ctgatgccgt tattaatgct aatttcaagg gagcaaaatg gtcagataac atttgggaca
     4981 gacaagataa gttacgtagc atcatatctc aaagcgtcca aagtgctatc ctaaaaggta
     5041 aaaatggctt aactattgca agagatatta gacgagagtt tgatgtgtca gcatcttacg
     5101 caaagcgact agcgataacg gagcatgcaa gggttcagat ggaagttggt agattatcca
     5161 tggcggagaa tggctttgct atgtttgata tattgcctga gcctaaagca tgtgatgttt
     5221 gcaaggatat agctaagcat ggtccatatc accttgacaa gtggagaata ggggaaaact
     5281 ctccgccgtt tcatccgtat tgtcgttgtg cagttgtagg attggataag aaacaagaag
     5341 aaaaagtgaa tgataaatct gaaaaatttg aaaacataac accgaatctc atgccattat
     5401 ttagtaagtt tgcaagtaaa ataacagatt tgcaacgtaa aatagtgtac tctgctgatt
     5461 tagcagatac cgggtacatt agaacaccgc atgcgtttga tataaacaat accttgcgaa
     5521 ataaaggcta caattatcta aacgttgatg ataaactaat taccgacaca ttggatagtg
     5581 tcatctcgat aaattcaact cccaaaaata taaaagtata ccgatttgat gattttgagt
     5641 tattaggttc aatcaacgaa caaaacaata atatttttga ttcaggtaat tttatggata
     5701 aattaaacca gggaggatta tcatacacca acgatggata tacttctgca agttatgatg
     5761 ttaaaaaaaa cgtgatggga tatcgtccta ttaagactga aataaaagta cctaaaggaa
     5821 gccacgttta tcttacagat aatgaggaag aaagtgaaat tatactacca cgagggacaa
     5881 agtatgatat aattaatgcg aagataaatg aatatgaaga aatagaaatc actatggaaa
     5941 taagaaagga gtaagcgatg gatttttctg attttttgaa taaaaaacaa aaagagtggg
     6001 atgaatctca tccaattcct gactttagtg caatgagtga tgaagaattg ctttatcagc
     6061 caatgagtga agctttagtg tctgaaagat ttgctaaaga gttatctaaa gaggttagaa
     6121 agcgtaattt attatcaaaa taaaaacata ttattataga cggaagactg tggtcgcccg
     6181 tctttttttg tacccaaaaa caggaggaaa acatgaatcg tgaaagtaaa ccaggtatgg
     6241 aaagcgtaaa aatcggaggg ttgacatact ctgttgaaaa ggcatcggac ctgcaaggca
     6301 aagatggcaa ttggggtctt atacactaca aaacacaaca aattaaacta gatgactcat
     6361 taaccgagca gctcgaagat caaacactta tccacgaaat tgttcatgga attttagttg
     6421 aggcaggcta tactaatcac gaagaggacc aagctaatcg cattggtctg attttgtatc
     6481 aagtattgca ggataatgat tttagctggt tgtacaaagg ggaataaata tgaataaacg
     6541 catcaagaaa aaacgtaagt tagaaacagc agttgtgatg cttgttgcag aaaatgctat
     6601 acaagcaaaa gctcttagaa atcaaaacaa acaaattgca gagctgagag cgattataca
     6661 acaaaacgtc caagcaataa atagagagtt tgtaactgct aaaaacacga ttttagataa
     6721 ccaattagct attaagttaa ttggtgatga tgttggtcac atcaagcaaa attataagcg
     6781 gaagtggcga aaataaattt taaactggtc gaattcgacc cctttagaat caaactaagt
     6841 cgtaggaata cggttttttt attatgtcca agcattgacg acgataaaag ctatggaatt
     6901 aataatcggg gacgatttaa aacataggag gtgccaatca tggcagaaga aacacaagta
     6961 gttgagatgg ttgaagagca agtggtacct gaagcaaaac aaccgcaaga cgaaaaaaag
     7021 tatacagatg cagatgtgga cgctatcatc gacaaaaagt ttgcgaaatg gaagtcagaa
     7081 caagaagctg agaaatcgga agccaagaaa atggctaaga tgaacgagaa agagaaagca
     7141 gactacgaaa agcagaagct gttagacgaa ttgcaagaac taaaaaacga taagacacgc
     7201 aatgagttaa cagcagtagc tcgtcaaatg tttgcagaat ctgaaatcaa cgtcaacgat
     7261 gacgtacttg gtttagttgt gactttggac gcagaacaaa caaaagcaaa tgtaacaacg
     7321 ctagcaaacg catttgctaa agttatcgct gatgaccgca aggctcttgt acgccagacc
     7381 actccgtcaa caggcggtgg attgaggaaa caaaccaatt acggtgctaa cttggctagt
     7441 aaggcagcac aacaaagcac caagcttttt taggaggaaa ttatgaacaa acgtaaagta
     7501 acaacatcta aagagattct acacaatctc gactatgaag ctatttcagt aactttggat
     7561 tcaaaaaaaa tcggcaagga agttgttcca gctgggacag tgttagcagg tgtttcagga
     7621 tcagtatttg aagaccgcaa acagaaagtt aaagcggtcg ataacagtgc cgtagaaagt
     7681 gaggataaca tctgcgggat tttgcttaca gatgtcgatt taacaaatgg cgatgcagtc
     7741 ggttcctgta tttatcgtgg gactatcaat gcagacaagc ttgctgattc atctattgcg
     7801 ggaaaatatg aagtgttgga agaagaacta ccgcacattg tctttatcaa gggaggtaaa
     7861 taaacatggc attgattcac gaaattatca catcggaaaa tatcaaaggt ttttacaatg
     7921 ctaaaaacga aaatgtcgga aacacgctag gggaaaacgc atttccccca aaacaacaat
     7981 taggactcaa actttcgttt attaaaggtg cagcaggcaa acctgtcaca cttaaagcgg
     8041 cagcctttga tacaaaagtg ccactacgtg accgtatgac tgttgaatta ctcgacgaag
     8101 aaatgccatt cttcaaagag gctatggttg tcaaggaagc agaccgtcaa caactcagtt
     8161 tgttagctca aactaaaaat caagagctta ttgacacagc gctagcagcg atttacaacg
     8221 ataaagtaac acttattgca ggtgcaaaag cacgtctaga agcaatgcgt atggaagtat
     8281 tgtctaaagg taaaatccat gttgcatcaa acggtgtcat gaaagatttt gattatgggt
     8341 tagattcttc gcaaacaact aagccagata caaaatggga acaagtagat accgctacac
     8401 cacttaaaga catcgaaaaa gctatcgaag taatggcaga acgtggcttt gtgccagaag
     8461 ctatcatcat gaactctaag acacttagcc tgattaaaaa tgcaagtaac actttagacg
     8521 ttgtaaagcc tatggcgcca gatggggcag ctgttactaa aggtgatttg gaaacttata
     8581 ttgctgatga attaggtctt aaaattttgc ttaaggatgg aatgtttgtc ggagacgacg
     8641 gtaaagctaa aaaatacttc ccagatggat ttgcaacttt agttcctaac ggcaatcttg
     8701 gttacacagt atttggtact actccagagc aatcagattt gcttggtggt gaggcaacag
     8761 atgctgaggt ctctattgtt gaaacaggta ttgcaattac cactactaag acaactgacc
     8821 cagtgaacgt ccaaacaaaa gtttctatga ttgctttgcc atcgtttgaa cgcttggaag
     8881 aagtacaaat cattaatgca acagaatccg aagaagaaac taaaaaagaa aacagctttg
     8941 aaatgtagga ggtcaatatg cctagagtaa ttagagcatt taaagataaa gtaacaaaag
     9001 tagtctacga agtcggcgat atttactcgg gcgaccgagt agagtttttg acagagggtg
     9061 gtgttttaga accgtccgta gactttgaca agctgaaagt gagtgagatt aaaagcaaac
     9121 ttgacgagtt aagcattgag tatgatgcta aacttaaaaa atccgagcta ttgaagcttt
     9181 taaagcaaac aatcggatag tttggaggtg tttatggatg cagtaaacac aagtagcgtt
     9241 ataagcaatg taaagcttga tttaggcatc ttagacagtc aacaggacga tttacttaac
     9301 atgttgctaa aacgcgttac agaccatttt aaagctaaat atggcgctgt cgaaatagac
     9361 agcgctttta gttttgtttt agaagattgt ttaattgcta gatttaaccg tagaggtgcc
     9421 gagagggcaa aaagcgagag tgtggaaggt catacgacaa catactacga ctttttggat
     9481 gagtttgaac cttacgatgc catgattatg gcaaagctta atttaatcaa agacaaatct
     9541 cgtaaagggg gactgtactt tttatgagat atgcagatag agttacattt gttaaaacga
     9601 cggatgagca atacaatccc gatttaggtg agtatacgca cacagaggtc ataagtatca
     9661 caaaaccttg ttttgtgatg gacatgggca tggaaaagtc cgtacagatt tttggagatt
     9721 atcaaaagga tcgtaaagtt atctacctga agcagcctta tacaaaagca tttgattatt
     9781 gcgagtatga gggcaagaga tacaaagcgc aggcaaataa gcttggcgct attgtttttt
     9841 atctggaagg agatgattct attggtggct gatatatctt taaaagtagt tggaacagct
     9901 ggtttaaaaa agaaacttga gcttattgtc aaaaaagatg ccgtcaagaa gattgtcaga
     9961 gacaatggga cgcagcttca aaggaaaatg attaataaag cggtatttac aaaaggctat
    10021 tcaacaggtg caactagacg ttctattacc atgcaaatcg gcgatggtgg attgagtgtc
    10081 aaggttaaac caggaactca ttatgccggc taccttgaaa gaggaactcg ccttatgagc
    10141 aaacaaccgt ttgttttgcc agctctaaaa gagcaaaaag taaaatttag aaaagatttg
    10201 gaggcgcttg tcaaatgatt aaaactagag atcagtctat ttttgacgaa atgtttaaac
    10261 gcatccagtc tttaggtttt aaggtttacg attataaacc gatgacggaa gttccatatc
    10321 catttgtgga aatggaatct actgatgcgg aatatattcc aaataaagat gacattaaag
    10381 gttctgttga acttatgttg tctgtttggg gggtgcagaa aaaacggaag caggtgtctg
    10441 acatggcatc tgctattttt tcgcaagcta tgtccgtagc tcgtaccgat ggattctgtt
    10501 ggtcgtttaa tattaggcag tcgtctatac agatgttaga tgatacaaca actgtgacac
    10561 ctcttaaacg agcgattgtc acacttagat ttaatttgag ataggaggta gtaatgtcag
    10621 aacctagcaa agcaacaaca caggaggaga aagaagtaat gtcaaaagca caagaacaaa
    10681 caaaacaatt agaagcaaaa caaggaattc attcaatctt gttatttcgt ttgttgaaag
    10741 aagcatctag tgaggtagca actaaacttg cttttcaaac agagcatgaa gtaggtaaaa
    10801 gccgtgacgt agatggacaa aaaactaaag atggtattat ccagtccgtg ggggctttgg
    10861 agtacgactt taaagcaaca tctattttag ccaaaggtga cgaactagca gctaaactag
    10921 aaaaagccat ggaggatggt gagcttgtag aaatttggga tattgattca gaagaaataa
    10981 gcaaaaacgg cgacagcgac aataagcttg caaaagtttg gggtattaat aaaaatggaa
    11041 caaacggagg aaacggtaaa tgcctagcga cttattacca agggtacatt tcaagcttca
    11101 gcgctaagaa aaacgcagaa gaaaatattg taattgaaat ggagtttgcc atcaatggtg
    11161 ttggtaaaaa agggttcgct acattaacag atgcccaaaa agcagctgta cagtacgcat
    11221 ttaaagatac aacaaaatcc aacagcgaag aaactaaaaa agaaaacagc tttgagatgt
    11281 aacggttagg ttggatttag tatccaacct tttattttta aaggagaaaa gaataatgca
    11341 attggaaatt aaaggtaaaa ctcataacgt aaaatttggt acacgatttg ttgccgagat
    11401 ggacaaaaat catatagcag aacgtcaggg atttaaattt ggagctggtc tacaatctag
    11461 cgttccgttt ttaatcgacc acagcgttgt tactcttgcg gaagtaattt atacaggtac
    11521 aatcaccgaa ccaccacgtc ctagtttgaa tgatatttac gactatattg acgaagttga
    11581 agatattgaa aagctttttg atgatgtttt agacgaacta cgtcaatcaa acgcatcaaa
    11641 gttgtttatg gctcaggtgg aaaaagacct ggcggaagcc aaagcgtaac aaatataaaa
    11701 aagagttatt cttctcaaga aagctttgag acgattgtgc ttaattgcat tagatacctt
    11761 ggcatgactg acatcaatga aatcgggcga ttaactttgt atgaatatga tttattaatg
    11821 acaggcaaag cactagcggc tgttgatgaa tcacataaag ctcacaaaca agcttggata
    11881 aaccaccaag ttacggcaac aaagcttgtt ggtggcaaga aaaataaaaa agaagtccct
    11941 gtttataaaa aattcaagga cttttttgat tatgaggaag aaatccgaaa aatcactcaa
    12001 gaaattgatg aaggttacga caagaaaggt atggatttac ttctcaaagc taacctttaa
    12061 ggaaaggagg ttaaatggga gaatcttatt ctgttgaagc ggttttgaca gctgttgata
    12121 aaacctttgg caaaacatta caatcggcaa tccgttcaat cgaaggcttg gaaaagcgtt
    12181 caaccggttt ttcatcggtg tctcaaaaag ctagttccat gtttaaatcc atgttaggag
    12241 cgaatttagc cggacaagct atctcggcaa tgacaaggac agtgtcatca ggccttggct
    12301 ctatgcttgg cgagatgaat agttcagcga aagcgtggaa aacttttgac gccaatttag
    12361 cggacattgg atttggaaaa aaacaaattt tggcagctaa aacggcgatg caagactatg
    12421 caactaaaac aatctactcg gcatcagata tggctagcac gtatgcacag ttagcagcgg
    12481 ttggcgtgaa agataccgga aagctcgtaa aagcttttgg cggtttagct gcatctgctg
    12541 aaaatccgaa gcaggccatg aagtctatca gtcaacaaat gacgcaagca gtaggaagac
    12601 caacagttgc atggcaagac tttaggataa tgctggaaca ggcgcctgca gggatggcta
    12661 aagtcgctaa atctatgggt aaaaatcttg atgaactcgt cgccgatatc caggcgggta
    12721 gggttaaaac cagcgatttt ttggaagcgg taaaaaaagc aggcaatgat aagagtttcc
    12781 aaaagatggc aactgagttc aaaactgttg accaagccat cgacggtatg cgagaaggct
    12841 tatccaacaa attgcaacca gcgtttgaaa aagtgaacca atttggaatt agagcgatcg
    12901 aagcaatcgg taaacaactc gataaagttg atttttctaa gtttgctagt aatcttggga
    12961 aattccttga aggaattaat atcgataaaa ttgtatctaa tatttcatcg gcgatttcat
    13021 ctgtcacttc aaaggttaaa gaattttggg acggtttcaa acaaactgga gcaattagtg
    13081 ctttttcagg agctttgcag agcgtttggg aagctttaaa aaatgtcgct agcgccatga
    13141 gcggaggaaa ttggaagact tttggagcaa cagttggagg gactgttaaa cacgtctcta
    13201 acttcgctaa agctgtttcc gatgttttag gaaagatgga ccctggcaga ctaagaagtt
    13261 ggatagctac cttcgccgca gtagctggag gctttaaatt attcgaaaaa ttaacggggc
    13321 aaagcgtcat tggttctttt ttggataaaa ttggcagcaa atttggcctc tttggaaaca
    13381 aagccaaaga aggaacagac aaagcctcta acggcgctag aagaagcggt ggcattatta
    13441 gccaaatctt cagcggcttg ggtaatatcg ttaagtctgc tggtacagcc atatcaacag
    13501 ctgcaaaagg tatcggagct ggtattaaaa ctgctttgtc tggaatcccc cctatcatta
    13561 gttctctagg aaccgcaata tcaacagttg cgcaaggtat aggcactggg ctagcaatcg
    13621 catttaaagg ccttggttct gctattgcca tggtgccgcc taccacatgg ctagctttag
    13681 gagcggcaat tttaatggtt ggtgctgctt ttgccttggc aggaactcaa gctgatggca
    13741 ttagtcaaat tttaagaacc gttggcgatg ttgttgtgca aatcttacaa caggtcactg
    13801 atagtctagc cactttacta cctattatcg caaacgctat tggctctatg ttgccaattg
    13861 tagctggagc tatctctcag attgtaggcg cagtagcggg cggattatct cagctcgtta
    13921 tagccgtttc aacaggggca tctctcgtta taggggcttt cacaggactt cttggtggta
    13981 tttctggggt tattaactcc attagcgctg ttatccaatc gctaactggt gtgattaccg
    14041 cagtattcaa tggcatagct actgttattt catctgtcgg ttcggctatc aaagatgtat
    14101 tgacgggtct aggaaccgct tttgaaggat ttggaaatgg tgtaaaatca gctctagaag
    14161 gtgttggggc agtaattgaa tcgtttggta gtgcagttag gaatgtcctt gacggtgttg
    14221 caaatatcct tgattctatg ggtactgcgg cacttaatgc aggccgtggc gtaaaagaga
    14281 tggctaaagg cattaaaatg cttgttgatt tatcccttgg agatttggtt gctacattag
    14341 cagctgtggc aagcggtcta gggaagatgg ctagctcagc tggcgaaatg acaacattag
    14401 gttctgctat gagcaaggta gccaatggta tgacacgtct agcaacaagt gctacgatag
    14461 caattactgg attaacagtc tttgccacca ccatggcaac tattaagaca gcagttgcaa
    14521 ctctaccgcc agtcctaacg atggcagcga gtgggtttac cacatttact actcaggcgg
    14581 tggcagcagt gactggattg gctgcaatta atgctccaat cactatgttt aaagctcaac
    14641 taatgacaat aacaccagct ctagcacaag ctggcgctgg ctttgccgcg tttgttgctc
    14701 aatcatcaac atttagtaca ggtttagcat ctgccggtcc tacaatagca gcgttcaatg
    14761 ctaatttgat gagcttatct gcaacaacag gagcgctagt tgcatcaata gctggtttat
    14821 cagctgtgct ttctgttgta tcagctggct ttagccaaat aggggcttct gcgacagcaa
    14881 ctgttggtca aatacaagct tttgcttcta gtacaacagt tgtttcgtca gcatttgcta
    14941 gcatgcaatc tatgattcaa tctgccatgg ctgcaatagt aagcagcatt ataacatcat
    15001 ttaatcaagc ggcctctcaa atgcaatcaa tcttatctcg aatgctatct caggctagga
    15061 catttgggtc tcaactagag caacaaatga gacaatcggg acagcgttca ggacaaaatc
    15121 ttgctcgggg actatcttct caacaaagtg ctgttattaa tgctatttct agcatggtta
    15181 atgctgcggt atcaagagcc aacgcgggag ctggtcctat gcgtcaagct ggagcgtaca
    15241 tcggacaagg gcttgcgcaa ggaatgtatt cagcgctagg agctgtaaca gctgcagcaa
    15301 acgcccttgt agcacaagcc gagagagcag caagagccaa ggcgatgatt cattcgccgt
    15361 caaggttgtt tgcaaaacga gttggtcaat atatcccgca aggggtagct atgggtatcg
    15421 acaaaaacgc tgatgtcgtt gacgactctg ttggcgggtt atttgatagc atcaatagct
    15481 ttgattttaa tatcgcagat agactggcta gcattggagc taaattccaa ggtgttatca
    15541 aatcagagag ttcacaatcg ttatcgcagc aacaagagtt cgtacataca gctcaaccag
    15601 cgtatataaa ctttagttta ggtggaaacg aatacgaagc atttgtaagt gacatcacta
    15661 atcaacaagc aaaaattgaa aaaatcagac taaagagaag cagctggtag ttgtttctct
    15721 tagttttttg aaaggagtaa aatgtacgaa tttaacgata ctatcagagg tactccgaaa
    15781 gttactttta atttaaagac aacaattggt gaaaaggtat tagaagaaga actcaataat
    15841 gatttaggta cctttaggac attgactgtt tctggtcgtg atgtggtgga cttagagcat
    15901 caaacaacaa gtgttttagg gcgaaatggt gagtatttcc acaatgccac agtcgaagtc
    15961 agaaaactag aaataaaagc taaaatcact ggaaaagata accagtcaat gcgtttgcaa
    16021 tatgaaaaat taaacagatt aattgttagt cacaatcaag tttttttatc attttcagac
    16081 gaacctgaca gaaattatct aggcatcttt aaatctaaag atgtcccaga agaagtttct
    16141 aacgagcaga ttataggact aacattcatc tgctacaatc cgtttaaaat gtctgatgta
    16201 aaaactaaaa aaggaacatc tatccaaaat ggtgggttat ttcaaacaaa gcctatcatc
    16261 actctcaacc tatcatcacc aacaaaagaa attaaattgt tgcatgtcga aagccagaag
    16321 tatatcagat tgactggaac ttataccact gacgaaatca agattgatat ggccacgggt
    16381 aaaattaccc agaacggtcg caacatcctt ggagatttag atatgattaa tagtaggtat
    16441 tttgagctat tacctggtaa taatacatta cagtgtgcca atgccgccat aaccgcagag
    16501 tttagagagg tttatctatg atatacctgt ttgataaact tgagcgattg gtcgctactg
    16561 ttggtactga tgacttgctc tcatggcact ttaaggtcaa aaacaatgat tgggaccagg
    16621 ctagttttga agtgccagtt gattatgacg tagagccatt tgtctacttt ggttttttta
    16681 actacgaccc taatcaaaaa gaagatgtct ttaagctctt taaagtcatt gattacaacc
    16741 tagaagatag caagttttac aaaggcttag acaaagctga gagtgatctt gataccattg
    16801 ccattatcaa agataagcgc tttagacaat cgtccgcaga tgcttgtatt gatggtgctt
    16861 tagagggtac gggttatcaa gttggcaaag ttgagggaat aacaaatgtt agaacactta
    16921 gttattacta catcagtcca cgagcggctc tgattaagat tgtggaagct tttaactgcg
    16981 agttcaatgt caggtatacc tttattaata acaaaatcac tagtcgttat atcgacctca
    17041 aaaagcgctt tggaaagcca acaggcaagc aatttgaaca tggcaacaac ctgttaaaag
    17101 tcgtctacga ggaatcaaca gatgacattg tgacttgtct gattggcaga ggtaagggtg
    17161 aggaaatcca acacgaagaa gctgagccta aagatgtcga gggacacttg ccacaggaag
    17221 aaaggcggca aggctacggt cgaagaatcg aatttactga tgttgtctgg tcggtcgaaa
    17281 aaggcgaccc gatagacaaa ccagctggtc aaaactttgt agcactagat agtgcaaggg
    17341 aagaatacgg cttgtctcaa aatggtgagc taaaacaccg ctggggtgtc tttgtcaatg
    17401 aggaaatcga ggataagaca gaactcttaa aagcaacttg ggaagaattg cagcgtttgt
    17461 caatccctat cagaatttac aaggcagaaa tcttagacat tggtccagag acgtggaaag
    17521 gcgactcagt agccattatc tatgatgagg taaaaatagc ttttgaaact cgggttgatg
    17581 agattgatat tgacaagctt aattttaaca ggtcagtcgt aacacttggt gattacagcg
    17641 ttgtccaaaa tcgtgagtca cggtctcgta aagaggctgt ccaaaacatg atagatgaat
    17701 ctttagagac tatcacagac ttaggaatga catttcagga atttttgcaa ggcatcgaaa
    17761 aacgcatcga gactggcaaa aaggagatgg aagacaattg gcgaaaagtt aaccttgaat
    17821 tcgataactt taaaaagaag gttgagcaag aaggcttgca attcaacacc ttgaaagaac
    17881 aaatcaaaga agttgatgaa cgcaccgaca aagagctaga ggaattccga gccaccctca
    17941 aaaacctagc gttaccagag gaagcgatta aaaaaatcac agaggctatc aaagttgatg
    18001 acatcccgtc tcttaagcaa aactttgatg acttaaaaaa caaggtcagc gagacaagcg
    18061 agacggcacg tctaaatgcc gagattatcg gtacagacgg taagacccgc tataacaaaa
    18121 atttattggt tggcgaccct aaccgtgtaa aaacctatga ccaagactat atcgaggtag
    18181 aagctaatga tggcggcttt aagcgtggcg agacctacac gattagcttt agtcaaacgt
    18241 gtgagctact caaaaaagta gctatcacat tgacgcaaat taacaacaag ggagttaagt
    18301 tagtactgac accaaccaaa gcaaaaatgg atgcacagac gtttgaggtc actaaagata
    18361 aacagtctat agaggtttat cctttgagct atacaggcgt tttaacaggt gattggtata
    18421 aatctaagtc tgtggagata aacgcctcag aggcgcagga attagctctt gagatggctt
    18481 ataaagagat tgcagatgcc aaaggtgcaa ctattatcgg caaacagtcc gataaaccaa
    18541 aaattatttt agatgggagg agggacagat gacatcagtc gaaacaatac caataaaaat
    18601 tgtctttgac cgaaaagacg cttcagaatg gcagtcaact aaccctgtca ttgacgaagg
    18661 tgaactagtc gtcgagctag acacccataa gctaaaggtc ggagatggta aaacaagcta
    18721 caatgacttg ccttactatg aaggtccgca aggagaatcc ataacaaaag tacaactatc
    18781 cgaaaatggt gacttgtctg tgtggattgg cgacaaagag actaagcttg gcaacatcaa
    18841 aggtcaaaaa ggggacaaag ggacaagtat aaccgacatc accaaagatg gtgagacact
    18901 cactatcaaa ctatcagacg acactcaaaa aatctttaat atccccaatg gccaaaaagg
    18961 ggatagaggt aagggcgtag agagcgctag gattgacgaa tccggtcatt taaaattaaa
    19021 aattgaagag gaatcagagt tagatcttgg aaacgttaaa ggtgagtcag gacctaaagg
    19081 tgatagtatt actatcacaa atcacaagcg tgtttcagat ggaacgcagg tatctttcag
    19141 cgacgggaca caaattgttg tcccaaaagg ggatactggt gatgtcaacg gcatcaatct
    19201 ggaagattat gtcaaaaaat ctgaacttaa aaacgtcggt tctgcagatg ttaaagctat
    19261 taatgacttt ttagggctat ctcaaaaggt gtttacaagc agctatagtt atacagatag
    19321 cttactaaaa agttatgcaa aacccagcta ttcggcgagt tggtatgtca acgaatctac
    19381 agtttctacc aaaaatggtg acaaagtatt gattacaata cataacacta ccacccaagc
    19441 agacaattat ttggaggtag cggtgactta tgttggtgct aactacgtga ctgctacctc
    19501 aacaggtcgc ttactgacta ctcctggtga agtcaaagta gtgacaaaaa agcaagcgga
    19561 aaaagattat gctgctaaaa aacataagca tgagattagt gacatagctg gtcttaatga
    19621 gcgcttgtct ggctatctca gacaagctga tatacagtca cagcttaata atatcggtaa
    19681 gctaaaagac acgcaaaccg gacaatatct tgaggttaaa gtggttgata aaggacaggt
    19741 gcctagcaat accagtggca tgatcgtgtt tgagaggtca taatttgtta aaagatttaa
    19801 ataatatcat tattaataac aaaacgattg atcgtattat ttgtaataat acgattgtct
    19861 atcagcgttc gtgggatctg ctttttgacg gtttgctgac aagcgctggg aaaaaattgg
    19921 atatttatcc tttttataaa atcgtaatca ataatgcatt ttttgttaaa acccccacaa
    19981 caaaaaacaa tacgattttt ttagcagggc ataaaatact agcattacaa gatagtcatt
    20041 tttgtttttt tagagcatta gaaaaaagtt tttttgtcaa aatttacggt aaaaattagg
    20101 aggtaacatt tgagtagaga cccaacactt ttaatagacg agtcaaattt aacaatcggc
    20161 tcagatggac gtgcttatta tacatttacg gctgatggtg acacaaaaag cgttaaaata
    20221 gccaataaca aatgtatcgg tacaactcgc tttaaccagc tcatgattga gcgagggggt
    20281 aaaccaacta actacgtggc gcccgtggtt gtcgagggga caggtaatcc gactggacta
    20341 tttaaagacc tcaaagagat tagcctcgag ttaacagata ctaaaaactc caaactttgg
    20401 tcaaaaatca agcttaataa tcaagggatg attgaagagt attacaacgg tacaataaaa
    20461 tctgagatta tcaaaaccgc agagggcacg cagcaacgta ttagtagcga gaccgataaa
    20521 aaacttgcgc ttatcaacga gacagtctca ggcattagac gtgagtacca agatgcagat
    20581 agacagctat cgtcaagcta ccaagctggt attgaggggc taaaagccac aatggccaat
    20641 gataaaatcg gtttacaagc tgagatacaa acaaccgctc aaggcttgta tcaaaggtat
    20701 gataacgaga tacgcaagct gtccgctaaa ataaccacta cctcgtcagg cactacagag
    20761 gcctatgaaa gcaagctcga tggcttacga gctgagttta ctcatagtaa tcaaggtatg
    20821 cgcgtagagc tggagtcaaa aatcagtggg ttgcaatcaa cgcaacaagc aactgccagg
    20881 caaatctcac aagagataag taaccgtgaa ggtgctgtca gtcgtgtaca acagggccta
    20941 gacagttatc agcggcgatt acaaaatgct gaagggagtt acagcagctt acaacaaact
    21001 gttagtggtt tacagtctga tgttaatagt ccaaacagta agcttaattc tcggattagc
    21061 caattagcta gtcaaattga ccaacgagtc acaagagccg atgtgacaag catcattaac
    21121 cagtctgggg acagtatcaa gttagccata cagagagctg gtgggattga tgctaagatg
    21181 tccgcaaaag agattgtctc agccataaac ctcaatggct acggtgtcag aatttctggt
    21241 gagcgtattg ctttagacgg caataccacg gttaacgggg cttttggcgc aaaaattggt
    21301 gagtttatca agctaaaagc cgaccagatt atcggtggga caatcgatgc aaacaaaatc
    21361 aatgtgatta atctcaacgc tagcagtatt gttggtttag acgctaattt tattaaagca
    21421 aggattgagt atgcgatcac aagtttgctt gagggtaaag ttattaaggc tcgtaatggt
    21481 gcgatgacaa tagacttgca aagcggtcaa atcaaccatt acacaaatga gtcagccatg
    21541 agacgtatcg atagcagtac agctagtcag tttataaaga tgacaaagtc aggctttata
    21601 tctgagatag gtaacatgca agctgcaatg acggttatag gctctaatag cgatgggtca
    21661 gaaaaccatg aaaataaaac cttcggaggc ataagaattt ggaacgggaa gtcatcttat
    21721 caatcgacaa gttttgttga attagtcggt aaccgtgtag caatctatgg aaataaaaat
    21781 cgtagtccat ggctctttga ctcaacaacg tcaggatatg cttacctcat tccacagaat
    21841 gaaagtggta taaagcatgc catagggaga gctgatcgta agatagacca aatccacgtt
    21901 ggagatatct atgtgcaagg cgaacgtgtc gctatgatgt taaaagattt agcgaatagg
    21961 attggctata taggtacagg tggctgggct aaccgcatag gataaattag gagaaacaat
    22021 gcaagaaaaa ttattaggaa aaattattaa cgatttaaca cttaaagtcg ctaatttaac
    22081 gctggaaaat gctcaattaa aagcacagca tgaaatcgaa ttggaagaat taaacgcaca
    22141 attggatgaa gcaacagcac cgaaggaaga aggtaaataa acatgagaaa ttggaaagta
    22201 acaggaaact acccacagtg ggacggtaca ggagcagtca ttgatacgca ggtaattatt
    22261 acagatgaca ggggtgcagt catcgcagag aagatcaaaa aagatttgag aacagctaat
    22321 gatgcagaaa ttatcgaggc tgctttagag gagtttaaaa agactgctta cgtcgaaatc
    22381 gctatgggcg aagccgtgca aaaagtagac gaccttgaaa aaatctcaca ggaaactgct
    22441 aagactgcca aaactgctca aacagctgct ggactagcta aggtgtccgc agagcgcacg
    22501 caaaagatga ttaacttaca aacgatccac atgttaacga gcggcggcaa gattgatcct
    22561 gacatttata aaggcatgct tgagctaatc gagccagcca aaaaaggtga gtatcaagcg
    22621 tatgatgtct ttacggtggt cgacagtact aaagaggaag acggtgaagc aggcgaaggg
    22681 aacctagtct tcgtacacgt taacgagccg tttacttatg aggcgcaaac tcttaaagat
    22741 ttggaatcag aggataaagt cacagtcatt aaatatgcgg atttggttaa acaagattag
    22801 aggtggttag atgatagatt ttgtacaaat tggtgctttt tgtggcgctg ctttgtctat
    22861 cttgggtgtt tggggattta tcgtcaatcc ctttaaaaaa gccatggaag ctaatgagtt
    22921 tgccatggct caactcaagg actcaattaa ggagttagct tatgagctta aaaatcttga
    22981 tcgtgaacgt gagattacca aaaaaattat cgacagacac gaggagcgtt taggtcgcgt
    23041 agaagacgaa gttattatca acaaggagcg tattattacg ctatttaaaa aaggagaaga
    23101 aaaatgaata actggtttaa aaaagtagca attaaaacaa ttaaaacaat ggcacaaacg
    23161 gccgttggtc ttatcgggtc aagcatgttg attacggata ttaactggcc aacaatgttg
    23221 tcagcagtgc tgttgtcagg actaacatgt atcctgatga atgtgtcaca aatcaaggaa
    23281 gaggaatagg tcatgcgagc aatcacacga ttagcattag ttatagcaat cgcaatactg
    23341 tatgtgccat tatctgtggt tgctttgatt ttttatccat ttttagataa ggagggataa
    23401 atggctacat atcaggaata taaaagtcgt tcaaatggca atgcttacga tattgatgga
    23461 tcgcttggtg cgcaatgttg ggatggctac gcagactact gtaagtatct aggactgcca
    23521 tacgcaaact gtacaaatac aggatatgca agggatatat gggaacaacg tcacaaaaat
    23581 ggtattttaa actactttga cgaagtagag actatgcaag ctggagatgt cgcaattttt
    23641 atggtagttg caggtgttac accgtatagc catgtggcta tttttgatag tgatgcaggt
    23701 agtggatacg gatggttttt aggtcaaaac cagggtggag caaacggagc atataattta
    23761 gtaaaaatac catattcaac gacttatcct actgcattta gaccaaaagt ttttaaaaat
    23821 gcagttactg ttataggtaa tataggacta aataaaggcg attattttat tgatgtatca
    23881 gcttatcaac aagcagactt aaccgctact tgtcagcaag ctggcactac taaaacgatt
    23941 atcaaagtat ccgagtcact cgcttggctg tctgataggc atcagcaaca agctaatact
    24001 agtgacccta ttggttatta tcactttgga cgatttggag gagatagcaa cttagcgcaa
    24061 cgagaagcag atttatttct gtccaattta ccaaccaaaa aagtctctta cttagtcatt
    24121 gattatgaag actctgcaag tgccgacaaa gaagctaaca ctaatgcagt tattgcgttt
    24181 atggataaaa ttgcaaacgc tggatataag cctgtttatt acagctataa accatttacg
    24241 cttaataata ttgattatca gcaaattatc gctaagtacc caaacagcat ttggatagct
    24301 ggttatccag actacgaagt acgaacagag ccactttggg agttcttccc ttcaatggat
    24361 ggtgtgcgct ggtggcagtt cacaagtgta ggagtagcag gtggtttaga taaaaatatt
    24421 gtattattag cagatgatag tagcaaagtg gatataccta agattgacaa accacaaagc
    24481 cagcttactt ttaatcaaaa gctagatact aatactaaat tagacaactc gaatgtacct
    24541 tactacgaag caacccttag aacagactat tatgtagagt ctaagccaaa cgcaagtagc
    24601 gctgataaag aatttatcaa ggcaggaact cgtgtaagag tctatgaaaa agtgaatgga
    24661 tggtcacgta ttaacgcttc tcagtctgac cagtgggtcg aagataagta tttatctaat
    24721 gcaacacaag tataaaatag gaggtaaagc tcctttagat aagacaaaac cgctcagata
    24781 atttctgggc ggttttttgt gtatgatgaa ttatttttca agataaatat cgaaatgact
    24841 aaagttcttc atattgataa ttctattatc tttatatttt gcaaaaatat ctgatctagt
    24901 cccttcattt ggtgagtcaa ataagtctat ttgctcatgt ttcccatctt ttgtgccaat
    24961 ttcgattctg c
//
LOCUS       DATUYR010000023        23775 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.023, whole genome shotgun sequence.
ACCESSION   DATUYR010000023 DATUYR010000000
VERSION     DATUYR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23775
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.023"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(262..411)
                     /gene="rpmG"
                     /locus_tag="VCI33_000868"
     CDS             complement(262..411)
                     /gene="rpmG"
                     /locus_tag="VCI33_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP2831803.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            complement(427..609)
                     /gene="rpmF"
                     /locus_tag="VCI33_000869"
     CDS             complement(427..609)
                     /gene="rpmF"
                     /locus_tag="VCI33_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEP2831804.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            829..2109
                     /locus_tag="VCI33_000870"
     CDS             829..2109
                     /locus_tag="VCI33_000870"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEP2831805.1"
                     /translation="MKLQKPKGTQDILPEDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRSAYRQTLIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDVYLA
                     VLGDSANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFANISEQLLS"
     gene            2447..4195
                     /gene="aspS"
                     /locus_tag="VCI33_000871"
     CDS             2447..4195
                     /gene="aspS"
                     /locus_tag="VCI33_000871"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEP2831806.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVNGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLIAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKGNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            4188..5129
                     /locus_tag="VCI33_000872"
     CDS             4188..5129
                     /locus_tag="VCI33_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP2831807.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            5149..6027
                     /locus_tag="VCI33_000873"
     CDS             5149..6027
                     /locus_tag="VCI33_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP2831808.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGVLSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGFVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     EELGRGVTYLTGEGAYSGRRKKVIYVALGPRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            6070..6942
                     /locus_tag="VCI33_000874"
     CDS             6070..6942
                     /locus_tag="VCI33_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP2831809.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(6969..7277)
                     /locus_tag="VCI33_000875"
     CDS             complement(6969..7277)
                     /locus_tag="VCI33_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP2831810.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(7365..9056)
                     /locus_tag="VCI33_000876"
     CDS             complement(7365..9056)
                     /locus_tag="VCI33_000876"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEP2831811.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKTKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYKPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHSVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            9347..9784
                     /gene="argR"
                     /locus_tag="VCI33_000877"
     CDS             9347..9784
                     /gene="argR"
                     /locus_tag="VCI33_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP2831812.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            9781..10133
                     /locus_tag="VCI33_000878"
                     /pseudo
     CDS             9781..10133
                     /locus_tag="VCI33_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623648.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            10120..12675
                     /gene="mutS"
                     /locus_tag="VCI33_000879"
     CDS             10120..12675
                     /gene="mutS"
                     /locus_tag="VCI33_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEP2831813.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRGLSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFDSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDHYRKVMREGTGWIADIEAKERQASGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKILATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKAHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(13072..13725)
                     /locus_tag="VCI33_000880"
     CDS             complement(13072..13725)
                     /locus_tag="VCI33_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831814.1"
                     /translation="MWSSIFNFKENIVKKKLVMMLTLLISICAISVRANESGDLFTTI
                     GQSHLPGVTVAPFKTGDTTINVNLDPYGYVYGFVTSSNGNKIKKTENKTYEIPCSILE
                     KQDNPNCEGRKWTVSGNRADGGGNYTVQLEKPLAEGDKVTLSFADDGNLYFGQLVFED
                     GKTHNERLQKERDAEEQQYAEVLFKRSIEEESKKTWHQRLSDSIQDQWWNFKDWLRG"
     gene            complement(13890..14273)
                     /locus_tag="VCI33_000881"
     CDS             complement(13890..14273)
                     /locus_tag="VCI33_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEP2831815.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLTYTELYQFDIEYHPTRRKH
                     LEISRECALEELDAIRYAINQLSKVEYRQILIECYLISEEKTQQDIMEELNRSQSWYY
                     ESKKRALLEFVEFYRDGALKNNVRL"
     gene            complement(14248..14610)
                     /locus_tag="VCI33_000882"
     CDS             complement(14248..14610)
                     /locus_tag="VCI33_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP2831816.1"
                     /translation="MSAKEQLKELKPLFALITLFEEQRDKDIELINAFHNPEAIRHIE
                     KGTAKQLLYLAKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEILDKLGYSRNIVF
                     KLHREALEVLDEQEERYS"
     gene            complement(15014..15502)
                     /locus_tag="VCI33_000883"
     CDS             complement(15014..15502)
                     /locus_tag="VCI33_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831817.1"
                     /translation="MLELSIENIIKPMKTQGKTRVTGSIGDQAIRIDLDGLVIHYNGQ
                     GLLLETIPGTYGGKRYFFVCPDCERRCRKLFKASHAFACGSCQKVHQATLNRSKTDCC
                     YYWQLAFKECLKVNPEARHIHGYYSRDDFPKRPKYMRLTKYLYHWRRFHYYMDKGDRY
                     WL"
     gene            complement(15576..16085)
                     /locus_tag="VCI33_000884"
     CDS             complement(15576..16085)
                     /locus_tag="VCI33_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831818.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGTYEQPVID
                     ATYHNDDQGGRANQLVLKYVEDRKLNKVFKRICDSFKDYYNQPEDMTDEELDHQRVVS
                     LSKSTQGKVNNRDTLIAFISDHNQLAEHLSMNYKEMTPEDLGAILESISQAFNHLYDM
                     VVEGQLLVK"
     gene            complement(16087..16260)
                     /locus_tag="VCI33_000885"
     CDS             complement(16087..16260)
                     /locus_tag="VCI33_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831819.1"
                     /translation="MKLKLHTRGGNTITIQGDRTLYNELVKYLLSGEQPNWVASPSAI
                     INLSDIIAITKEK"
     gene            complement(16266..16439)
                     /locus_tag="VCI33_000886"
     CDS             complement(16266..16439)
                     /locus_tag="VCI33_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEP2831820.1"
                     /translation="MKIKLFYQKHNESLDDFEHRVNLFTLSVSVIDIKFQEATYGNYE
                     DMDSRTGLLVLYR"
     gene            complement(16436..16690)
                     /locus_tag="VCI33_000887"
     CDS             complement(16436..16690)
                     /locus_tag="VCI33_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831821.1"
                     /translation="MNPNYTNHGVKKMRYNYDDLPNDQLYRLELPNGQCGYDFITKIN
                     FNIKSRPGDCDFTRPELTRRGYQWVFDCGFAIPVEKGAKT"
     gene            complement(16984..18378)
                     /locus_tag="VCI33_000888"
     CDS             complement(16984..18378)
                     /locus_tag="VCI33_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEP2831822.1"
                     /translation="MKDYQNKLSQATQPAFAPAFRTRKGRGDKEYVISSPYNVGKVFE
                     FYENIYTGIKYNEFEKTIEITKAVPWSKEKGLWTNEQTSLCIAFIDEKYRFTPRKEHI
                     EVAITALAKKNTYHPIKQRIESQKWDGKARGERYFIDLLGCADNSYNREIAKVWLTGL
                     MARIYLRKVKFEVVPILIDKRQGTGKSTVTKRLLPSYHTDSEIKFGKNDSDYQKIQAN
                     AIIELGELKGMSKAEIETVKSFISSDSDTYREPYERKATPHPRHCVFIGTANKKSFLK
                     DSGTERRFFPIECGINDVKKHPMEVEEDYFLQVLAEAKVWFNNYEPLTPSKELMNQLA
                     DIQEDYKVEDVDKEIIDQLLNEFQIVEGWDSLSQYEQRQYILKQLGEPLDNAQSYSDY
                     PSAQTDCLLQVTSPNHIAYLGFNQKPTQGGKALISQKIRDYLDNDDGWKKGENPARKR
                     LFKGGTPVPYYERV"
     gene            complement(18390..19256)
                     /locus_tag="VCI33_000889"
     CDS             complement(18390..19256)
                     /locus_tag="VCI33_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEP2831823.1"
                     /translation="MAIYEARGFSSYLYPYKGPLEPFDYVAQFRPLKPPEDIDIEEYK
                     RTQAPYCLSGKVTAEKNGSYKRNNASLVYRDLIFLDYDEIETGVNLPKIVSQTLWEYS
                     YIIYPTIKHTPEKPRYRLVMKPSDVMTEATYKQVVKEIADKIGLPFDLASLTWSQLQG
                     LPVTTGDPEDYQRYVNHGLDYPVPKNGSTPNRQVVTTYTPRPRSQRSITMRVIDTLFN
                     GFGDEGGRNVALTKFVGLLFNKWVDCDLETAYELVQIANSVTTKPLPIDEIDTTFRSI
                     LDKELRKRGIKP"
     gene            complement(19257..19529)
                     /locus_tag="VCI33_000890"
     CDS             complement(19257..19529)
                     /locus_tag="VCI33_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831824.1"
                     /translation="MNVFSQDYEAKLLEQNLTAFNHFLEAYQKPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLRRYQPPLEDTRKHYYKVSDILIFLGVNV"
     gene            complement(19532..19861)
                     /locus_tag="VCI33_000891"
     CDS             complement(19532..19861)
                     /locus_tag="VCI33_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831825.1"
                     /translation="MTLPENYRRVLNLIKVGADNPITGAEISLILKLEERSVQSIISS
                     LITRYNVPIIGIRHGFNRGYFIPANKEELLDGAKAFYNQVQKEQERLSVLLNADLTSY
                     KELLKGG"
     gene            complement(19873..20064)
                     /locus_tag="VCI33_000892"
     CDS             complement(19873..20064)
                     /locus_tag="VCI33_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831826.1"
                     /translation="MQELNLTPTQTLILFFVLGLLGLLLSRSKPLIEIDLPEDTHAPK
                     PRQNANYGAYIQAQNHYYN"
     gene            complement(20064..20396)
                     /locus_tag="VCI33_000893"
     CDS             complement(20064..20396)
                     /locus_tag="VCI33_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831827.1"
                     /translation="MRYRVETNPFSKDRYTPEQLEMFKNRQLSKDKAEVYFTRLYNQH
                     IAWVIIANVMTEYVIKFRKSATSFEEAWDALDYQRTTEIVFRAVNGLPCSEKDSGELE
                     TYLSEVSA"
     gene            complement(20498..20950)
                     /locus_tag="VCI33_000894"
     CDS             complement(20498..20950)
                     /locus_tag="VCI33_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831828.1"
                     /translation="MLKNQDNMIKKKRNPRVLFRNVAYKLSEIEGKTLTEIASFLGFG
                     NSEVCRSTLYKWKRKKWLKFDLKNGHYRNVEVLHEVTLEKMANKELKEQGLIYKANIY
                     YEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELFAELYTNMN"
     gene            complement(21091..21264)
                     /locus_tag="VCI33_000895"
     CDS             complement(21091..21264)
                     /locus_tag="VCI33_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEP2831829.1"
                     /translation="MSPKMGRPVKGTAKRDKRLEVRLTADEYNTIQETADKNGLSKAD
                     LIVKAVNSYESEK"
     gene            complement(21482..21655)
                     /locus_tag="VCI33_000896"
     CDS             complement(21482..21655)
                     /locus_tag="VCI33_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEP2831830.1"
                     /translation="MTDTKKRVGRPKGENSNTVKLTVRVSEELDSKLNKYAKENNLTK
                     PETLRKAFENLTK"
     gene            complement(21903..22430)
                     /locus_tag="VCI33_000897"
     CDS             complement(21903..22430)
                     /locus_tag="VCI33_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEP2831831.1"
                     /translation="MTMRYLLEAFGVLSFEIHKPEKGSLGGRPRRIYHLNEQQATLLV
                     TYLGNTEPVREFKKNLVKAFFEMREELTQIRLQRSLEAPKRKTLNEAIKTWEHAPKMA
                     YPTVYNLLLKAVTGKNSKQLKVTRGGYSDIDCLNSIELAQYTALEDMAIALINLNFTY
                     QDIKTMALKNTLQCA"
     gene            complement(22842..23249)
                     /locus_tag="VCI33_000898"
     CDS             complement(22842..23249)
                     /locus_tag="VCI33_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831832.1"
                     /translation="MARTPFTQELLHQIFDDTGTMSLELIAERLPDWSEKDIKLRLAA
                     WRYRNNIDYTMANGEIDTFEIINNRKAISEEVSAGRQLKLEEYFKQVQATAEIINKPT
                     ASDTNRLKAIQLQQVAMDEIPDQYFKELTELYG"
     gene            complement(23270..23470)
                     /locus_tag="VCI33_000899"
     CDS             complement(23270..23470)
                     /locus_tag="VCI33_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2831833.1"
                     /translation="MTVGDKIKTIRLNLGETMEEFGKRFGTSKGTVNNWEKGRNLPNR
                     NSLLRIAQLANQTVSDFLAITD"
     gene            complement(23504..23710)
                     /locus_tag="VCI33_000900"
     CDS             complement(23504..23710)
                     /locus_tag="VCI33_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2831834.1"
                     /translation="MTQITLKAARINAGYTLKQVAGAVGKNPQTISKYEKDSSDISLG
                     LLQKLSSLYGVTIDNLFLGKKVRK"
BASE COUNT         6710 a         4320 c         4786 g         7959 t
ORIGIN      
        1 aattttaaca ttaaactttg tagatgtcaa tttcagctac accaaaatat agataagaag
       61 ttagtgtacc aaatataaaa aagccctgcc atccaaatga taacagggct taacttcaat
      121 atccaaatga tatattcatc taaaaaaggt agagtaaaag gtagagttct tattgattta
      181 tattacaatg taatgaactt caaaaattca aaaatcgctt aaaatcaacg ttttgacgtc
      241 taatgacgtg tagtgaaccc cttacttaac ctctgtaaat gtaacgtgtt tacgtaattt
      301 tggtgagtat tttttcaatt gaagacggtc tggagtgtta cgtttgtttt ttgaagtaag
      361 gtacaagcgt tcaccagatt ctttatgttc aagtgtaata tttacgcgca tggtatctcc
      421 cttctattat ttagcttcgt tagctttagc gattttacgt cctttgtagt atcctttaag
      481 ggatacacgg tgagaacgtg agtaatctcc agtagtttcg tcgaattgta cagatggagc
      541 tgtcaatttg tagtgtgtac gacgtttgtt tttcttcgct tttgacgtgt gacgtgcagg
      601 tactgccatt ttcttattct tcctttcgat gtgtaagttt tgattttcat caacttcact
      661 atgataacat atcttttttg taaagtaaag ttacttgaca aaacttttta tttttacaat
      721 ttcatggtga aaatactatc aaactaaagc aaatgatata tgcttgttta aaatataaga
      781 aaaaatggta gaatgtttaa agagaaaact aatcaaagag gtaaacttat gaaacttcaa
      841 aaaccaaaag gaacccagga tattcttcct gaggatgctg cgaaatggca gtatgtggaa
      901 tctgttgcgc gtgatacgtt tagccaatac aattatggag aaattcgtac tcctatgttt
      961 gaacactacg aggtaatctc gcggtctgtg ggtgatacga ctgatattgt gacgaaggaa
     1021 atgtatgatt tctatgataa aggagatcgt cacattacct tgcgaccaga gggtacagct
     1081 ccagtggtac gttcttatgt tgaaaataaa ttgtttgcgc cagaggtcca aaaacctgtc
     1141 aaactttatt atatcggttc catgttccgc tacgaacgcc ctcaagctgg tcgtttacgt
     1201 gaatttcacc aaattggagt agaatgtttc ggtgctgcta atccagcaac agatgttgaa
     1261 accattgcga tggcctatca cctctttgaa aaattgggta ttaaggatgt cacccttcat
     1321 ttaaatagtt tggggagccc tgagagtcgt tcagcttatc gtcaaacctt gattgactac
     1381 ttaacaccaa tgcgtgatca attatctaaa gatagtcaac gtcgcttgga tgaaaaccca
     1441 ctgcgcgtgc ttgattctaa agaaaaagaa gataaactag ccgttgaaaa ggcgccttct
     1501 attttggatt acttagatga ggaaagtcag gcgcactttg aggcggtcaa agatatgctt
     1561 gaagcacttg acattcctta tgtgattgac accaatatgg tacgtggcct tgattactat
     1621 aaccacacta tttttgaatt catcacgtct gtggagggct ctgacttaac catctgtgcg
     1681 ggtggacgtt atgatagttt ggtaggttat tttggtggac cagaaacccc tggctttggc
     1741 tttggtcttg gtttagaacg tctcttgatg attattgaaa agcagggcat taccttgcca
     1801 attgagacag agatggatgt ttaccttgct gtgttaggtg atagtgctaa tagcaaagct
     1861 ttagaattgg ttcaagccat tcgccgtcaa gggtttacag ctgaacgaga ttaccttggc
     1921 cgtaagatta aggctcagtt taaatcagcc gataccttta aagcaaaatt ggtaatgact
     1981 ttaggagaaa gtgaagttga agcaggcaaa gctgtgatta agaataatcg tagtcgtcaa
     2041 gaagtggaag tcagttttga agatatgatg acaaactttg caaacatctc agagcaatta
     2101 ttgtcttaat gattttattg tcgtcataag cttttgttga tatgattgtt tagaagataa
     2161 tcactaaaac aggtgtaact tcccttttta gaagggagtt gcacctgttt gttttctgta
     2221 aggggattga ttagctttct tagtattctg tgaggacacc tgatatgtca aaatgctgac
     2281 gactcagtat tcttgcttta tctttagaaa aactcttttt aaatgggtgg ggtttttaag
     2341 ggctttttgc tataatagta gggaatgaat taattgattt atatagtgaa aggaattgga
     2401 aacgagcagg tttcacagga ttctgtttgt tttagggaat ttatttatga aacgtagcat
     2461 gtatgcaggg cgtgttcgcg aagaacacat tggaacgaca atcacattga aaggatgggt
     2521 cagtcgtcgt cgtgacttgg gtggtctcat ctttattgat ttacgtgacc gtgagggtgt
     2581 gatgcaatta gtcattaacc ctgaggaagt ttccagtgat gtgatggcaa ctgctgaacg
     2641 cctacgtagt gaatatgtga ttgaagtgga aggttttgtt gaagctcgac aacaagccaa
     2701 tgataagttg gcgacaggaa tggttgagtt aaaagtatct gctttgacca ttttaaacac
     2761 agctaaaacg acgccttttg aaattaaaga tgacgttgaa gtaagtgatg acacgcgttt
     2821 acgttaccgt tacttagatt tacgacgtcc agaaatgtta gaaaacttca agctacgtgc
     2881 aaaagtgact cattctatcc gtaattacct tgatgacctt gagtttattg atgtcgagac
     2941 acctatgttg acaaaatcaa ctccagaagg tgcgcgtgac tatctggtac caagtcgtgt
     3001 tagccaaggg catttctatg ctttgccaca aagtccgcaa attaccaaac agttattgat
     3061 gaatgctggc tttgatcgct attatcaaat tgtgaaatgt ttccgcgatg aagatctacg
     3121 tggtgaccgt caacctgagt ttacacaagt tgacttggaa acgtcattcc tttctgaaca
     3181 agagattcaa gacatcgttg aaggcatgat tgctaaagtg atgaaagaga caaaagaaat
     3241 agatgtaacc ctaccattcc cacgtatgag ctacgatgta gcaatgaata gctacggctc
     3301 tgataaacca gacacacgtt ttgagatgct tttacaagac ttgacagtaa ctgtcaatgg
     3361 aattgacttc aaggttttct cagaagcacc agctgtcaag gcgattgtgg tgaagggaaa
     3421 tgcagatcga tactctcgta aagatattga caaattaact gagtttgcca aacaatttgg
     3481 tgctaaaggc cttgcctggg ttaaggtgac agatggtcaa ttggcaggcc cagtagccaa
     3541 gttcttgata gctattgaga cagagttgtc aagccaatta aaacttgctg agaatgattt
     3601 ggttttattt gtagcggata ctcttgaggt tgctaataat acattaggtg cgcttcgtaa
     3661 tcgtattgcc aaggaccttg acatgattga tcagtcgcag tttaatttcc tttgggtggt
     3721 tgattggcca atgtttgagt ggtcagaaga agaaggacgt tatatgtcag ctcatcaccc
     3781 cttcactctt ccaacgccag aatctgctca tgagttagag ggtgatttgg ctaaggtacg
     3841 ggcaattgct tatgacatcg ttttaaatgg ttacgagctt ggtggtggta gcctgcgtat
     3901 caatcaaaaa gagatgcaag agcgcatgtt caaggctctt ggctttaccg ctgatgaagc
     3961 taacgaccaa tttggtttct tactagaagc gatggactac ggctttcctc ctcatggtgg
     4021 cttggcaatt ggtcttgacc gttttgtcat gttgcttgct ggaaaaggca atattcgaga
     4081 agttattgcc ttccctaaaa ataacaaagc ctctgatcca atgacgcaag cacctagtct
     4141 tgtttctgaa aaccaattag aagaactcag tcttcaaata gaaagtcatg attaagaaaa
     4201 caacctataa gaaaaaagtt aaatatgtca ttagtagagg ggccaagaaa gttggcctac
     4261 tccacgctct aagaagtatt tcaagagaaa aatatgcaga gaagatttcg gcttctctgc
     4321 tttatggcat tctctctagt attgctgtga attttttctt ccagcctggg catgtttatt
     4381 caagtggagc aactggtcta gcacaggttt tttcagctct tagtcatcgt cttttaggct
     4441 atgattttcc catcgccttt gcgttttatt tgattaatat tcctttgctt attttagctt
     4501 ggtataaaat tgggcatcaa tttaccattt ttacctttat cacagtcagc atgagttctt
     4561 tctttattca aatcatgcct caagtgacgc tgacgactga tcctcttatc aatgctattt
     4621 ttggtggttt ggttatggga atgggaattg gtacaggtct caaatcacgt atctctagtg
     4681 gggggactga tattgtcagt ttgaccctta gaaaacgaac aggcaaggat gtgggcagtc
     4741 tctcattgat ggttaatggt gcaattttag cctttgcagg gattttattt ggctggcagt
     4801 acgcccttta ttctatggtc tctatctttg tatcaagtcg tgttacggat gccattttca
     4861 ccaagcaaaa gaaaatgcag gcaactattg ttaccagcca tccagagcgt gtgattcata
     4921 tgatccataa acgtctgcat cgcggggtga ccagtatcaa cgacgcagaa gggacttaca
     4981 agcatgaaca aaaagcagtt ttgattacca ttttgacatg tgaagaatac ccagaattca
     5041 aatggctgat gttaaaaaca gacccacaag cctttgtttc agtggctgag aatgttagaa
     5101 ttatcggtcg ttttgtggaa gatgattaag aaaaacacag gaggtgtcat gtcaattgac
     5161 caacgtttac agttaaagaa attttgcttt attattttag gagctgctat ctacgccttt
     5221 gcttttgttt atttttacat ggctaatcgc attgcagcca atggtttagc tggtctcacc
     5281 ctagttggta aggcactttt tgagattgat ccttctttga taggctattt gattaatttg
     5341 cctttggtct tgctgggagc tagatgtttt gggaaaagag ccatggttta cacagtaacg
     5401 ggtgttttat cactttactt ttttgtgtgg ctgtttcaac gctttccttt ggtcgttgac
     5461 ttggaccatg acaatttagt cgtttccttg atggctggaa ttattggtgg tctaggtggc
     5521 ggttttgtgt ttcgcaatgg tggaaccatc ggtggagcag atattattgc taagttatta
     5581 gaagacaagt taggactaca gctgaatcaa gctcttttag gcattgatct ttttgtcatg
     5641 attgtctctt tgacttatat ttccctgccg caaatgatgt atgctctgat tgctagcttt
     5701 atgtatggtc aaattgttcg cttggtacaa caaggtggtt attcagcccg tggcattttt
     5761 atcgtttcag aacaagcgga gaaaattgcc cggttcatca tggaagaatt gggaagggga
     5821 gttacttatc taacaggtga aggagcctac tctggtcgcc gtaaaaaagt gatttatgtg
     5881 gctttggggc caagagatat tagggaatta aaagcttttt taactcaggt tgatcctaat
     5941 gcctttatca cttattttga tgttaatgaa gttaatagcc cagaattttt aactattaaa
     6001 agcaaatacc ataaaaagag aaaataaatg agcattagag ttgcctgcga agaatagtga
     6061 ggataaagga tggcacatca cgataaattg actaaactac taaaactgtt tttgattgcc
     6121 ctaggtgtag ccatttatac cttcggtttt gttaatttta acatggctaa tgctttggct
     6181 gaaggtggtg tggcaggaat aaccttgatt ttacacgcgc attttgggat taatcctgct
     6241 tactcttctc tcttatttaa tcttccactt tttattttag gggcaaagat ttttgggaag
     6301 cgttctttgg ctctaaccat ctatggaaca gttctgatgt ccgcttttat ctggatgtgg
     6361 caaaaagttc ctatcgaact tggcttggaa aatgacatga tgttggtggc tgtagtggca
     6421 ggtctctttt cagggattgg gagtggcatt gtttttcgct atggtgcaac cacaggtgga
     6481 acggatatta ttggtcgcat tgcagaagaa aaatttggag ctaagctagg tcaaactttg
     6541 cttttggtgg atgctcttgt attgacagct tcgttgactt atgtggattt aaaacacatg
     6601 ctttatactt tggtggcaag ctttgttttt agccaaatga ttagtgtggt tcaaaatggt
     6661 ggttacacta tccgtggaat gattattatt accaagcatt cagaggctgc cgctcaagct
     6721 atcctaaccg aaatcaatcg tggagtgact tacttgaaag gtcaaggagc ttactctggt
     6781 aatgattaca atatcatgta tgtgaccttg aacccaactg aagttcggga agtcaaacgt
     6841 attttagctg gtttggatcc agatgccttt atctccatta ttgatgtgga cgaagttatt
     6901 agctctgatt ttaaaattcg ccgaagaaat tatgataaat aaaaatacag gtcaaacagc
     6961 ctgtattttt attttggtcc cccacttaag aaactaccaa gcataatagc tgatatagca
     7021 gttcccttgt aatggctagc ttttgttttt accaagcgac taagatcaaa tatgctttta
     7081 ggagcatcaa attgatgtgt taagatgtat tgattgataa gtgagtcaat ttttgtagct
     7141 aggagtgagc tcatcccctc tttttcaagt tgactggcga gctcgaagat cgcttgacga
     7201 aaataatcat cggcttgtat tacttgttcg ctataaactt tactaagagc atctaatatt
     7261 tctttttctg atggcatggc attctcctgt aacatattgt gctattatta taatataaaa
     7321 aactaagact gtcataaatg tgacagcctt agtaatgtta ttacttacat ttcatttggt
     7381 gcgtcaacac cgagtaagcg aagcgcttct ttaagaacag tagcagttgc atagcagaga
     7441 gctaagcggt tatcacgttc tgagttgtcg tcaagaatgc gagtgtgagc ataatatttg
     7501 ttaaagcttt gggctaagtt aatagcaaat ttagccataa tagaaggctc aaagttatct
     7561 gatgttcgct tgataatgcg tgggaaatct tggataagtt tgataatttc ccagctttca
     7621 gcatcagcta aactgtaagt ggtggttgca gatggcgtaa agtcagcttt acgtagaata
     7681 gattggatac gagcatgagc atattgaacg taaggtccag tttctccttc aaaggaaacc
     7741 atggcttcaa ggtcaaagtc atacccattc atacgatctg ttttgagatc gtaaaacttg
     7801 atggcaccga caccaacaga gtgtgcaacg gcttctttgt cagcaaggtt tggatttttc
     7861 gcttcaattt gagaagctgc acggttaatg gcttcagcaa ccgtaggttc aagaagaata
     7921 acattacctt tacgtgttga taatttagca ccgccttttg tgacaagtcc aaaagcaacg
     7981 tgtgtcatat catctgacca atcgtaaccc atttctttta gcactgcttt taattgtttg
     8041 aagtgagcag cttgctcatt accaacaaca taaacggatt tagcaaagtc ataagtgcgt
     8101 ttacggtaaa gtgcggcagc taggtcacgg gtaatgtaaa gagtagcgcc atctgatttt
     8161 ttgatcaagg cagggtgttc aatgccatat ttttcaagat taacaacttg ggcaccttta
     8221 gattctacaa gaaggttttt agcttctaat aagtcaagaa cctcatccat tttgtcatta
     8281 tagaaggcct caccgttgta actatcgaag gtgacatgta attggtcata aagtcggttg
     8341 aactcaagta aactttcatc acggaaccat tgccataatt cagtagcttc tttgtcgcca
     8401 tcttccaatt tacggaacca ttcacgagct tcttcatcaa cggtgggatc agtttcagct
     8461 tcagcattga tacgaacgta aagttttaag agttcatcaa taggatgtgc ctgaacagca
     8521 gcttcatctc cccatttttt gtaggcaaca atgagcatac caaattgttt accccagtca
     8581 ccaaggtgat tgatttttac aggtttatag cccattttgg caaaaatgtg agcgagacta
     8641 tcaccaataa ctgttgagcg gaggtgaccg attgaaaatg gcttagcgat gtttgggctt
     8701 gacatgtcaa tagcaacatt tcgcccttga ccttcgtctt gctgagcata gtctgaacct
     8761 gctgtaatca cttgttcaag gacctgtgag gaaatcttag ttttatcaag gaaaaagttg
     8821 atgtaagggc cgacagctac gactttttca aattggctct catcaatctg ttcagctaac
     8881 tcgctagcaa tcatttgagg ggctttgcga aggacttttg ctaaactaaa ggctggaaag
     8941 gcaaggtctc ccatgtcaga attttttggg gtttcaagca agttaaaaat agcatcttgt
     9001 tctaattctg gaacaacttt agctatttca cttgcgatta gagttttagt atccatacag
     9061 cgtctcctaa aaattgtttg tcaccctatt ctaacatatt tctttaaaat aaacgaggag
     9121 aggattgata aattatactg aaactttcaa ggaaatgaat tgcttgaggt tgttgttttt
     9181 ctgaaaatgt tataaaaaga gaaatgaggt ttcttttcaa cagttttcaa gtgaaattct
     9241 agttgaattt gctaaaaaat tgctgaaaat agctgggcga agatgaaata atacactttt
     9301 tttgttataa tttgagagga taattataca attggggtga tatatcatga ataaaatgga
     9361 gcgtcaacag cagattaagc gaatcattca agcagagcat attggcacac aagaagatat
     9421 taaaaatcac cttcaaaaag aaggcattgt ggtcacccaa gcaactctat ctcgggactt
     9481 aagagaaatt ggccttttga aattgagaga tgaacaaggc aaactctatt atagtttgtc
     9541 tgaacccgtt gcaacaccat ttagtcctga ggtgcgtttt tatgttttga aagttgatcg
     9601 agcaggtttt atgctggttc tccataccaa tttaggagag gctgatgttc tagctaattt
     9661 gattgacaat gatgccattg aagatattct aggaaccatt gctggtgcgg ataccttgct
     9721 tgtgatttgc agagacgaag agattgctaa acgctttgaa aaagatttgg ctgcaggcct
     9781 atgactagct attaagagtc tttgacacaa ttgattcttg ctatccatga attctagtat
     9841 cgttgcttat aaagaagtgc agcaatagct gtctaagctc cgtgatttta aaaagtaagc
     9901 ttatcagatg aaacgctatc aacaagatgc cctgcttttc aaaaaaaata gataaagaaa
     9961 aggctgcgac agtatctgca agcagggcaa aagaactaga agataggctc agtcatcagc
    10021 cattaattga tgattatcga gaaaagatgc aagatgcaag tgatgtgatt cagtatatca
    10081 ccaaacgtat agaagatcag ttaaacaagg agttaacaaa tggcaaaaac taacatttct
    10141 cctggaatgc aacagtatct ggacatcaaa aaagattatc cagatgcttt tttgcttttt
    10201 aggatgggtg acttttatga attattttac gaggacgctg tcaaagcagc acaactctta
    10261 gaaattggtt tgaccagtcg caacaagaat gcggaaaatc caattcctat ggcaggcgtg
    10321 ccacatcatt ctgcccaaca atacattgat gtgttaattg agttgggtta caaggttgct
    10381 gtcgcagaac aaatggaaga cccaaagcaa gctgttgggg tggtgaagcg tgaggtcgtt
    10441 caagtcataa ctcctggaac ggttgtggat tcagctaagc cagatagcgc caataacttt
    10501 ttggtagctg ttgactttga tggttgccgt tatggattgg cttatatgga tgtgtccaca
    10561 ggtgaatttt gcgtgacaga tttggcggac tttacgagtg ttcgtagcga aatccaaaac
    10621 ctcaaggcaa aagaagtctt actaggtttt gatttatctg aagaagaaca gacgattttg
    10681 gtcaagcaga tgaatttgct gctttcttat gaagaaacgg tctatgaaga taaatcttta
    10741 attgacggcc aattgacaac ggtagaactg acagcggcag gaaaactctt gcaatacgtt
    10801 cacaaaacac aaatgcgagg actcagccac ttgcaagcat tggttcacta tgagatcaag
    10861 gattatttgc agatgtcgta tgccactaag tcaagtttag atttggtaga aaatgctagg
    10921 actaataaaa aacatggaag tctctattgg ctgttagatg aaaccaagac agctatgggg
    10981 atgaggcttt tgcgctcatg gattgatcga cctttggttt ctaaagaagc tattttagag
    11041 cgtcaagaaa ttattcaagt ttttctgaat gcttttattg agcgaaccga tttaagcaat
    11101 agtttaaaag gtgtttacga catcgaacgc ttatctagtc gcgtgtcttt tggtaaggca
    11161 aatccgaaag atttacttca attggggcat accttagccc aagtgcctta tatcaaagct
    11221 atcttagagt cttttgacag tccttgtgtt gacaaacttg tcaatgatat tgacagtttg
    11281 cctgagttgg aatacttgat tagaacagcc attgatccag atgcaccagc aactattagt
    11341 gaaggcagta ttattcgtac tggttttgat gagcgcttgg accattatcg taaagtgatg
    11401 cgagaaggaa caggttggat tgcggatatt gaggctaaag agcgtcaagc aagcggcatt
    11461 aataacctaa aaattgatta caataaaaaa gatggttatt attttcacgt tacgaattca
    11521 aatcttagct tagttcccga gcattttttc agaaaggcaa ctttaaaaaa ttctgagcgt
    11581 tatggaacag cagaattggc taagattgaa ggtcagatgt tagaggctag ggaagagtca
    11641 tctagtttag aatacgatat ttttatgtgt attcgagctc aagttgaaac ctatattaat
    11701 cgtttacaga aactggctaa aattttggca acggtagatg ttttgcaaag tttagcagtc
    11761 gttgctgaaa ccaatcatta tatccggccg cagttcaatg ataatcatgt gattacaatt
    11821 caagaaggtc gtcacgcggt tgttgaaaag gttatgggag tgcaggaata cattcccaat
    11881 agtatctctt ttgaccaaca gaccagtatt cagctgatta caggtccaaa tatgagtggt
    11941 aagtcgactt atatgagaca gctggcctta acggttatca tggcccagat gggttcattt
    12001 gtggctgctg accatgttga tttaccttta tttgatgcga tttttacgcg tattggggct
    12061 gctgatgatt tgatttctgg gcaatcaacc tttatggtgg agatgatgga agcaaaccaa
    12121 gcaatcaaac gcgcaagtga caactctctt attctatttg atgaactggg acgaggtacg
    12181 gcaacttatg atggtatggc tttagcccag gcaattattg aatatatcca tgatagagtt
    12241 ggtgctaaga ccatatttgc aacgcattat catgaattga cagacttgtc aactaagttg
    12301 acaagtctag tcaatgttca tgtagcaacg cttgaaaaag atggcgatgt taccttcctt
    12361 cataagattg ctgagggacc ggcggataaa tcttacggta ttcatgtggc aaaaatagca
    12421 ggactgccaa aatccctatt aaagagagca gacgaagttc tgacccgttt agaaacacag
    12481 tcacgatcta ctgagataat atcagtccct tcacaagttg agtcaagcag cgctgttaga
    12541 caggggcaat tatccctttt tggtgatgaa gagaaagctc atgagattag gcaagcactg
    12601 gaagctattg atgtcatgaa catgaccccg cttcaagcaa tgacaaccct ttacgaattg
    12661 aaaaagttgt tatagtcttt cagctgaaaa tgaaaaagat gctttctata ttgaaggcat
    12721 ctttttgttc tgtcaaaaac ggtccgaggc cttcggctat ttattaagtg tgttataata
    12781 gtccataaga atgcgaggaa attatgacaa acattattgg acttggattg gcagtcttta
    12841 cagggtattg acagtcctta agcattgtta tgactgtgtt tttgaaaaaa tataggtttt
    12901 aagtttcctt agacttccct caaaagtcca caaaaaggtg aacaaaaaaa gacctttaca
    12961 ggtcctttac acaatgagtt cagcaggcaa gaactagcgt ggtttaaata ctacgctttt
    13021 tagtttgccc tatggcttat tatagcacga ttagtgtcat tgaggaaaaa actaccctct
    13081 caaccagtct ttaaagttcc accattggtc ttggatgcta tcgctcaagc gttgatgcca
    13141 agtttttttg gattcttctt cgatagaacg cttgaataac acctccgcat attgttgctc
    13201 ttcggcatct ctctcttttt gaagacgttc attatgtgtt tttccatcct caaatacaag
    13261 ttgcccaaag tatagattcc cgtcatccgc aaaagataag gtaactttgt ctccttcagc
    13321 aagtggtttt tccaattgaa ctgtgtagtt gcctcctcca tcagcacgat tgccacttac
    13381 tgtccatttc cttccttcgc agtttggatt atcttgtttc tccaaaatag agcaaggaat
    13441 ttcataagtt ttgttttcgg tttttttaat tttattacca ttacttgaag ttacaaaacc
    13501 ataaacatac ccatagggat ctagattaac attaatggtt gtatctcctg ttttaaatgg
    13561 tgctacggtt actccaggta agtgcgattg ccctatagtt gtaaacaaat cgccactttc
    13621 attagctctg acacttatag cacatattga tattaacaaa gttaacatca taactaattt
    13681 ctttttcact atattctcct taaaattaaa tatcgaactc cacaatacca gttaatcagt
    13741 caaaaaatcc attctacttt ttgatatttt atgtctaatt tgacccgcta agtcagattt
    13801 gcaaacgata ccaagtgatt ttcttctaat cattattttt tagatggata tgttaattgt
    13861 attagagacg tattaatctt aaaccattgt cataagcgaa cattattttt tagcgccccg
    13921 tctctgtaaa attccacaaa ctcaagcaag gctcttttct tagattcata ataccagctt
    13981 tgacttctgt taagttcttc cataatgtct tgttgagttt tttcctcact gatcaagtaa
    14041 cactcaatta gtatttgtct atattctact ttagacagtt gattaatggc atacctaata
    14101 gcgtctagtt cctctagggc gcattctcgg cttatttcaa ggtgttttct gcgcgtggga
    14161 tggtactcta tatcaaactg gtaaagctcg gtataagtta aatcaaggct attagcgata
    14221 cgttgccatc tatgaaattc tttcagctta cgaatagcgt tcttcttgct catctaacac
    14281 ctctaaagct tctctatgta gtttgaacac aatgtttctt gagtaaccta gtttatcgag
    14341 tatctcgtcc catgataagt catccacgta tctagcctta ataacagcta tctgtctctc
    14401 atcttggagc gtggcgatca tggctagtct cttatcacgt tctttagcta aatataagag
    14461 ttgtttagct gtaccttttt cgatatgtct tattgcttca ggattatgaa aagcatttat
    14521 cagctcgatg tctttatctc gttgttcctc aaataaggtt attaaagcga aaagtggttt
    14581 caattcttta agttgttctt tagcgctcat ggtcttttct cctggttatg gtataattta
    14641 tttaagctta aatttaacca agggggcgtt cgtatggacg tctttttgtt ttgttcgttt
    14701 tgtaaactag atattttttg tagtacttcg cttttcgctt ctaaaatcgt ttctaagcac
    14761 tttttagagt ttatagtata aatcatcaac ttagtagcta gaagcgctaa aacaatgttt
    14821 tattttagtc ttagatggtt attctttgtg gcagggttat gggctaaaat gagatgtcaa
    14881 atcctttcct atcaagcttt ttaatcttcg tcaaaaattt caaaaaggga attttttgca
    14941 cggaaaaggg cgcgttctta agtttccgaa caatatagtc ccgtttaaaa atgaaggggg
    15001 tagtttccga atattatagc cagtacctgt cacccttatc catatagtaa tgaaatcttc
    15061 tccagtgata aaggtatttg gttaatctca tgtactttgg acgtttagga aagtcatcac
    15121 gactataata tccatgaatg tgtcttgctt ctggatttac tttcaaacac tctttaaagg
    15181 ctaattgcca gtagtagcaa cagtctgtct tgcttcgatt gagcgtggct tgatgaacct
    15241 tctgacaaga accacaagca aaggcatgag aagccttaaa taatttccga caacgtctct
    15301 cacagtcagg acacacgaaa aagtaacgtt taccaccata agttcctgga atcgtttcaa
    15361 gcaataggcc ttgaccattg tagtgaatca ctagcccatc taggtctatg cggatagctt
    15421 ggtcacctat tgatcctgta actcttgtct tcccctgtgt cttcattggt ttaatgatat
    15481 tctcaataga tagttctaac attatttctc cttaaaactc aaacaaccca aaactattga
    15541 taaaacgaca aaaagaggaa aaaccctctg aatgtctatt taacgagtaa ctgaccttca
    15601 acaaccatat catacaaatg gttaaaggct tgactgatag actcaaggat ggcccctaag
    15661 tcttctggcg tcatctcttt ataattcata gagaggtgtt cagccagttg gttgtgatcg
    15721 gagatgaagg ctataagtgt gtctcgatta ttaactttcc cttgagtact tttagataaa
    15781 gaaaccacgc gctgatgatc aagttcttca tccgtcatat cctcaggttg gttgtaatag
    15841 tccttgaagc tgtcacagat acgcttgaag actttgttta gttttctgtc ttcaacatat
    15901 tttaagacta actgattagc acgaccacct tggtcatcat tgtgataagt tgcgtcaatc
    15961 actggttgct cataagtacc agtcatatag cctaaaatag catgacaagc cacttgtgcg
    16021 gtgtcaaagt ctttaaacgt gtagtggaat gtgaatgtct ttggtgtgtc tgaaaatgtt
    16081 ctcatgctat ttctcctttg tgattgctat aatgtctgat aaattgataa tggcagaagg
    16141 agatgctacc cagtttggtt gttctccaga taaaagatac ttgaccaact cattataaag
    16201 ggtgcggtct ccttgtatgg tgatggtgtt accacctcgt gtgtgtaatt ttagtttcat
    16261 atcagttacc tgtacaagac cagtagtcct gttcttgaat ccatgtcttc ataattgcca
    16321 taagtggctt cttggaattt aatgtctatc acagataccg atagggtaaa gagattgacc
    16381 cgatgttcaa aatcatctag tgattcattg tgtttttgat aaaatagttt gattttcatg
    16441 ttttagctcc tttctcaaca ggtatcgcaa agccacagtc aaagacccat tggtaaccac
    16501 gtcgtgttag ttctggtctg gtaaaatcac aatcacccgg tctagacttt atgttgaaat
    16561 taattttggt gataaagtca taaccgcatt gaccattagg aagttccaat cggtatagct
    16621 ggtcgtttgg caaatcatca taattgtaac gcattttctt tacgccatga tttgtatagt
    16681 taggattcat atgtccctct tttctctcta aaatgtgtgc tttttgtttt tttaaaaatc
    16741 aaatagcaca gcaaaaagca cagggataaa gccagtggta tcaagggggt tgggcacttt
    16801 gtgcttttag tagcattaat ttgagcaaaa gactttcttt ttttgcacgc gcactattag
    16861 ttataaatat tattatctat aatattaaat actactaata ctacaataga gtataaagcc
    16921 tataatacca aggttttagc ttgtatcatt acgtgtagtt tttatgtagc attagtgtag
    16981 tttttaaact cgttcatagt aaggtacggg agttccacct ttaaatagtc tttttcttgc
    17041 gggattctct ccttttttcc agccgtcatc attatctaag taatcacgta ttttttgaga
    17101 aataagggcc ttaccacctt gcgttggttt ttggttaaat cctagataag cgatatggtt
    17161 aggacttgtc acttggagta agcaatctgt ctgtgcggaa gggtagtcac tatagctttg
    17221 agcattatct aacggctctc ctagctgttt gaggatgtat tgccgttgtt catactgtga
    17281 taagctatcc caaccttcaa caatttgaaa ctcattcagt aattgatcga taatttcttt
    17341 gtcgacgtct tcaaccttat aatcttcttg aatgtctgct aactgattca ttaactcttt
    17401 agatggcgtt agtggttcat aattgttaaa ccatactttg gcttcagcga gtacctgtaa
    17461 gaaataatct tcttccacct ccataggatg ttttttcacg tcattgatac cacattcaat
    17521 agggaaaaag cgtctttctg ttccactatc cttaagaaaa gattttttat tagctgttcc
    17581 gataaagaca cagtgccttg gatgaggagt ggctttacgt tcataaggtt cacgataagt
    17641 atcactatct gaggaaatga agcttttaac tgtttcaatt tctgcctttg acatgccttt
    17701 tagctcccct agctcaataa tggcattggc ttgtatcttc tgataatcac tatcattttt
    17761 accaaacttg atttctgaat cagtgtggta gctagggagt agtcgcttag taacggtgct
    17821 tttcccagtt ccttgtcttt tatcaatgag aataggaacg acttcaaact ttactttacg
    17881 gagataaatt ctagccatga gacctgttag ccatactttg gcaatttctc tattatagga
    17941 attatcagca cagcctaata gatcaataaa gtagcgttct cctctagctt taccatccca
    18001 tttttgactt tcaatacgct gtttaatggg gtgataagtg ttctttttag ctaaagcggt
    18061 aatagctacc tctatatgtt ctttacgagg ggtaaaccga tatttttcat caatgaatgc
    18121 aatacaaagg ctggtctgct cgttcgtcca tagccctttt tctttagacc aaggaactgc
    18181 tttagtgatt tcaatagttt tttcaaattc gttgtattta atacctgtat agatattttc
    18241 gtaaaattca aaaaccttac cgacattgta ggggctacta atgacatatt ctttgtctcc
    18301 tctacctttt cgtgtcctga aagcaggggc aaaagcaggt tgagtggctt gcgatagttt
    18361 attttgatag tctttcaatt cttctttgtc tatggcttga ttcctctctt tcttaattct
    18421 ttatcaagta tgcttctaaa ggttgtatct atctcatcaa tgggtagtgg cttagttgtc
    18481 acgctgttag ctatttgtac cagctcataa gccgtctcta aatcacaatc cacccattta
    18541 ttaaatagca agccaacaaa cttagttaag gccacgttgc gcccgccttc gtctccaaaa
    18601 ccattaaaca aggtatctat gaccctcatg gtaatagaac gctgacttct agggcgtggc
    18661 gtgtaagtag taacaacttg tctgtttggc gtgctaccat ttttaggaac aggataatca
    18721 agaccatggt tcacatagcg ctgatagtcc tctgggtcgc ctgttgtaac gggtaagcct
    18781 tgtaattgcg accaggtaag actagctaaa tcaaacggca gtccaatctt atcggctatc
    18841 tccttgacca cttgtttata agttgcttca gtcatcacgt cactaggctt catgacaagg
    18901 cgataacggg gcttctcggg ggtgtgttta atcgttggat aaataatata actatactcc
    18961 caaagcgtct gagaaacgat tttaggtagg ttgacgcctg tttctatctc gtcatagtca
    19021 agaaaaatca aatcgcgata aactaaacta gcattattgc gcttatagct accgtttttc
    19081 tctgctgtga ccttgccact taggcagtag ggggcttgtg ttcgcttgta ttcttcaata
    19141 tcaatatcct caggcggttt caaaggtcta aactgagcaa catagtcaaa tggttctaaa
    19201 ggtcctttgt aggggtacaa ataagagcta aagcctcttg cttcataaat agccatctac
    19261 acatttaccc ccaaaaagat aagaatatca ctgaccttgt aataatgttt cctggtgtct
    19321 tctagtggtg gttggtatcg tcttaaccca gcattttccc accgttttag ggttttacct
    19381 ttgatattta attcctcttt gacttgttcg gccgtgatca accctaaaac tcttggttta
    19441 ggtttctggt aggcttccaa aaagtgatta aaagcggtca ggttttgttc taagagtttg
    19501 gcttcataat cttgactaaa tacgttcatg cctaacctcc tttgagtaat tccttataac
    19561 tggttaaatc ggcattcaat aacacactta ggcgttcctg ttccttttgt acttgattat
    19621 aaaaggcttt agcaccatct agtaattctt ctttgttagc tgggataaag tacccacgat
    19681 tgaatccgtg tctaatgccg ataataggga cgttatagcg cgtgattaag ctactgatga
    19741 tactttggac ggagcgttct tcaagtttca gtattaagct aatctctgcc cctgtaatgg
    19801 ggttgtctgc tccaaccttg atcagattaa ggacacgtct ataattctct ggtagtgtca
    19861 ttcagttcct ccctaattgt aataatggtt ctgtgcttga atataagccc catagtttgc
    19921 gttctgacgt ggtttaggtg cgtgggtatc ttctggtaag tcaatctcta ttaacggctt
    19981 agaacggcta agaagaagcc ctaagagacc taaaacaaag aatagaataa gcgtctgtgt
    20041 tggtgtgagg ttaagttctt gcatcatgcc gatacctcac ttaaataagt ttctagttcc
    20101 cctgagtctt tctctgaaca aggtaaaccg ttaacggctc taaagacaat ctctgtggtt
    20161 cgttgatagt ctaaagcgtc ccatgcttct tcaaagctgg tggcactttt tctgaattta
    20221 atgacgtact ctgtcataac gttagcaata attacccaag caatatgttg gttatatagt
    20281 cgagtgaaat agacttcagc tttatctttg ctgagttggc gatttttgaa catttctagc
    20341 tgttcaggag tgtatctatc ttttgaaaaa ggatttgttt ctactctata tctcattatg
    20401 ttttttctcg cttaattatt attttctgtg tagtgttttt attgattgct tgtttcttat
    20461 actagattca tgctagtttt aaggggtagc tccctgatta gttcatgtta gtgtataatt
    20521 ctgcgaataa ctcgctaggg atacgctcta gcgctttttg ttgtaagtgg atggctttaa
    20581 ttctatcttg tgttttggtt ttaatgtctt ctataatttc agatgttgag accacttgtt
    20641 catagtaaat gttagcttta taaatgagtc cttgttcttt taattcctta ttagccattt
    20701 tttcaagcgt aacttcatgt aatacttcaa cattacgata atgaccgttt ttgaggtcga
    20761 attttagcca ttttttacgc ttccatttat atagggtgct tctgcaaact tctgaattcc
    20821 caaaaccaag aaaagaagcg atttctgtta atgtttttcc ttcaatttca gatagtttat
    20881 aagcgacgtt tctaaataac actctcggat ttcttttttt cttaatcata ttgtcttgat
    20941 ttttgagcac acaaaaagcg cactcccttt ctatgaattt taggttcaca aaatagagta
    21001 cgcatgatat actatttacg tacctacttt gtgggtgctg ggagttccta acgtgtacgg
    21061 tcgccaaact ataccacgtt aggaactttt ttatttttca gactcataag agtttacagc
    21121 tttaacgatt aaatccgctt tagataatcc atttttatcc gctgtttctt gtattgtatt
    21181 atactcgtca gcggtcaaac gaacttccag ccgtttatca cgtttagctg taccttttac
    21241 aggtctgccc atttttggac tcataggagt tcctcctttc atttaagccc gtgcttatat
    21301 catatgataa gcacgtacat aagtcaaccc ctaaattaaa aaaacatgcg tattctattt
    21361 tcaatgtgca aatggttcac aaaatagagt acgcatgata tactatttac gtacctactt
    21421 tgtgggtgct ggctctatca cgttgacggt ttggcgattg taaacgtggt agagtacttt
    21481 tttattttgt aagattttca aaggcttttc tcaaagtctc gggtttagtc aagttattct
    21541 ctttagcgta cttatttagt ttgctatcta actcttcact aacccttacc gttaatttaa
    21601 cagtatttga gttttctccc tttggtcgtc ctactcgttt tttggtgtcg gtcattgagt
    21661 ttatcactcc tttcatgtcg acaaaaacta ttatagttta tgtcgtcaat aattgtcaac
    21721 ccctaaacca aacttttttt gcgtactcta ttttattttc aatgatcagt atgatacaat
    21781 ggaagtatca aatatttact aaaacccctt taataatagc ttgcctgctt tattaattga
    21841 gtttagttat actagttgaa ggcttggaag tttggtcgct gtcaaagcct tttttgttgt
    21901 tttcacgcgc attgtagcgt gttttttaat gccattgtct taatatcttg ataggtaaaa
    21961 ttaagattga taagagcgat tgccatatct tctaaagcag tgtactgtgc tagttcaatt
    22021 gagtttaagc agtcaatatc agaataacca cctctggtta cttttaattg cttgctattc
    22081 ttaccagtaa cagctttaag caatagattg tacacagtcg gataagccat tttaggcgcg
    22141 tgttcccatg tcttgatagc ttcattaagt gtttttcgtt taggagcttc aagagaacgc
    22201 tgtaacctaa tttgagtgag ttcttctcgc atttcaaaga atgccttgac taggttcttt
    22261 ttaaactctc tgactggttc ggtatttcct agataggtaa ctaataaagt cgcctgctgt
    22321 tcgtttaagt gataaattct tctaggtcgt ccgcctagtg aacctttttc aggtttatgg
    22381 atttcaaatg acaaaacccc aaacgcttct aaaaggtaac gcatggtcac gcaaagttac
    22441 gcagaatttt ccatgttgcg taacctactc aatctcttta gtaccaatcg atttcagcat
    22501 ttatttaaga aaggttacgc agtaacaaac aataagtcta attaatgatt tattaattat
    22561 ttatttatat ataggggtag ggtagggtga ttttgcgtta ctgcgtaacc atccttctct
    22621 aacgctatct atatcaacgt tttagaggtt acgtagtaaa aaagggggtg cgttacctta
    22681 tgtaccacac aacagcactt tcgttcttaa tcctttttat gcttatcaat cagctttcta
    22741 actgaaataa tgtcaatttc agcatgttca gctatgatgt cgtgtgtggt gtaaggctct
    22801 ttcttaccat ccatgtaaac taggttcatg gtaatcctcc tttatccata gagttccgtt
    22861 agttctttaa aatactgatc aggaatttca tccatagcca cttgttgtaa ttgaatggcc
    22921 tttaaacgat tggtgtcgct agcagtcggt ttattaataa tttcagccgt tgcctgtact
    22981 tgcttgaaat actcttcaag cttaagttgc cttcctgcgg aaacttcttc ggatatggct
    23041 tttctgttat tgataatttc aaaggtatca atttcaccgt ttgccatggt gtaatcaatg
    23101 ttatttcgat accgccaagc tgccagccta agcttgatgt ctttttcaga ccaatcggga
    23161 agccgctcag caatcagctc taggctcatt gttcctgtat cgtcaaatat ctgatgtaat
    23221 aattcttgtg taaatggtgt tctagccatt tgttatttac ctgcctttct taatcggtga
    23281 tagctaagaa gtcgctaaca gtttgattag cgagctgtgc tattcttaat agactgtttc
    23341 tattcgggag gtttcttcct ttctcccaat tattaacagt acctttagaa gtgccaaagc
    23401 gttttccgaa ttcctccata gtttctccta agttaaggcg aatagttttg attttgtcac
    23461 ccactgtcat ttcttgcctg cctttctaat taataaagta ctattatttt cgtacttttt
    23521 tacctaaaaa aaggttatca atagtgacac cgtacaaaga tgataatttt tgcaatagtc
    23581 ctaaagaaat atcggagcta tctttttcgt actttgaaat agtttgggga tttttcccaa
    23641 ccgctccagc tacttgtttt aaagtgtaac cagcgttgat tcgggcagct tttagtgtaa
    23701 tttgcgtcat gttttcaact cctttctaaa aagctatggc ttaatagtac tattattttc
    23761 gtaccaagtc aatga
//
LOCUS       DATUYR010000024        23084 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.024, whole genome shotgun sequence.
ACCESSION   DATUYR010000024 DATUYR010000000
VERSION     DATUYR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23084
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.024"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            57..275
                     /locus_tag="VCI33_000901"
     CDS             57..275
                     /locus_tag="VCI33_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2831835.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
     gene            complement(346..489)
                     /locus_tag="VCI33_000902"
     CDS             complement(346..489)
                     /locus_tag="VCI33_000902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831836.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            576..796
                     /locus_tag="VCI33_000903"
                     /pseudo
     CDS             576..796
                     /locus_tag="VCI33_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            1498..1881
                     /locus_tag="VCI33_000904"
     CDS             1498..1881
                     /locus_tag="VCI33_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP2831837.1"
                     /translation="MKVHTTLEKDENGNWYRKDQTKTPAGERLIELDDVTIVVLENWR
                     RNQVVNTDTDFIISRFGEPFCKSTICRVIKHKAQEVGVPVITGKGLRHSHASYLINVL
                     KKDILYVAKCMGHADKSTTLNTYSH"
     gene            2144..3379
                     /locus_tag="VCI33_000905"
     CDS             2144..3379
                     /locus_tag="VCI33_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP2831838.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVDRTYCRRKILKKGSLVLERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(3495..4457)
                     /locus_tag="VCI33_000906"
     CDS             complement(3495..4457)
                     /locus_tag="VCI33_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP2831839.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMFPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            4789..6066
                     /locus_tag="VCI33_000907"
     CDS             4789..6066
                     /locus_tag="VCI33_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP2831840.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTGFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRQYWKKHIEKNVLLTH"
     gene            6101..6685
                     /gene="folE"
                     /locus_tag="VCI33_000908"
     CDS             6101..6685
                     /gene="folE"
                     /locus_tag="VCI33_000908"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEP2831841.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            6694..7494
                     /gene="folP"
                     /locus_tag="VCI33_000909"
     CDS             6694..7494
                     /gene="folP"
                     /locus_tag="VCI33_000909"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEP2831842.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWVDPGFGFAKSVQQNTELLKGLDRVCHLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            7501..7860
                     /gene="folB"
                     /locus_tag="VCI33_000910"
     CDS             7501..7860
                     /gene="folB"
                     /locus_tag="VCI33_000910"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEP2831843.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGEKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            7857..8357
                     /gene="folK"
                     /locus_tag="VCI33_000911"
     CDS             7857..8357
                     /gene="folK"
                     /locus_tag="VCI33_000911"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEP2831844.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEFYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            8507..9394
                     /gene="murB"
                     /locus_tag="VCI33_000912"
     CDS             8507..9394
                     /gene="murB"
                     /locus_tag="VCI33_000912"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEP2831845.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVVDGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            9440..10594
                     /locus_tag="VCI33_000913"
     CDS             9440..10594
                     /locus_tag="VCI33_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2831846.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWVFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            10578..11372
                     /locus_tag="VCI33_000914"
     CDS             10578..11372
                     /locus_tag="VCI33_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831847.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            11369..12145
                     /locus_tag="VCI33_000915"
     CDS             11369..12145
                     /locus_tag="VCI33_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2831848.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            12138..13211
                     /locus_tag="VCI33_000916"
     CDS             12138..13211
                     /locus_tag="VCI33_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP2831849.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(13266..13931)
                     /locus_tag="VCI33_000917"
     CDS             complement(13266..13931)
                     /locus_tag="VCI33_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEP2831850.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIIENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(13912..15453)
                     /locus_tag="VCI33_000918"
     CDS             complement(13912..15453)
                     /locus_tag="VCI33_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2831851.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTRPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLFLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKTGILQLCNQAGQELIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSVTKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGWQH
                     ERFNH"
     gene            15614..16945
                     /locus_tag="VCI33_000919"
     CDS             15614..16945
                     /locus_tag="VCI33_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEP2831852.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILITG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            16977..18143
                     /locus_tag="VCI33_000920"
     CDS             16977..18143
                     /locus_tag="VCI33_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEP2831853.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDITNKHDLSIAYTPGVAG
                     VSSAIAKDKTLAYDLITKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLQKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEADQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPGDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(18225..19163)
                     /locus_tag="VCI33_000921"
     CDS             complement(18225..19163)
                     /locus_tag="VCI33_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEP2831854.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTYRPVGKNHSICGTDLHIL
                     GGDVPACKEGTILGPEGIGIVKEIGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSSH
                     CEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVLP
                     SHVKPGDNVCIVGAGPVGLAALLTVQLFSPANIIMVDLSQNRLEAAKTFGATHTICSG
                     SSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFNL
                     DELWIKNITLNTGLVNANTTEMLLNVLKVIIDNDIS"
     gene            19399..20130
                     /gene="aphA"
                     /locus_tag="VCI33_000922"
     CDS             19399..20130
                     /gene="aphA"
                     /locus_tag="VCI33_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEP2831855.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            20308..21840
                     /locus_tag="VCI33_000923"
     CDS             20308..21840
                     /locus_tag="VCI33_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEP2831856.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVMVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGNWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            22003..22617
                     /locus_tag="VCI33_000924"
     CDS             22003..22617
                     /locus_tag="VCI33_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEP2831857.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQAYI"
     gene            22776..>23084
                     /locus_tag="VCI33_000925"
     CDS             22776..>23084
                     /locus_tag="VCI33_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP2831858.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     I"
BASE COUNT         6989 a         3879 c         4864 g         7352 t
ORIGIN      
        1 acaattaaac attttttaat caaatctcct ttatactgat attcggagaa tgttagatgt
       61 atccgcgtat taggaatttg agagaagata acgatttcac tcaaaagttt gttgcaaatt
      121 tactttcctt ctctcatgca aattatgcaa aaattgagag aggagaggtt gccttaatgg
      181 cagatgttct tgtgcagttt tctaaactct ataacgtcag tattgattat ttattagggc
      241 aaacagatta tccgtatcgt tttcataata aataaaagtc tcgccttgat ttgttcattg
      301 acaactgaat aaaccgaggt gggactttct tgtttgtcga gcaatttagt gagatacgcc
      361 gtgataggag cgataattat tagctataaa atagagtggt tctctatctg ccatgaccac
      421 aaatagggac aatgatactt ctgaacgttc aggctgccag cgtaaaaggc cagtgggtga
      481 gattcccatg agtgatttaa ccagtcatac ccacggaggt tgtaattttt ttgaaaggag
      541 tggaggtgtg aaaatagtaa catataaaaa gcttgatgaa tattggtttt ctcgaacata
      601 ctgcaagaaa tattattaga gaagcaaaaa gaatagctat aaaaaagttg aagaagctcg
      661 cgaacttgaa caaaatgcgg tacaattaag taaatcgccc tttgacaata gaagacttgg
      721 tattgctcct aaagatattg tagaagagtt agtaggtgtt ctactctctg aagaaagttt
      781 agaaagtgag aattaaagat gactataaaa aatcaaaaaa agtacaaagg tgtttattgt
      841 gataagaatg ggaagatatt ttatcaagct gatcttggtg tagatccagt aacaggtaaa
      901 cgagttcaaa agaaagccag aaagaattag tatggaaagc catttggtac aatgaaagaa
      961 gcgtatgatg aactggttcg gattaggtat gagttcagta ataatcttag tattgaaaat
     1021 gacaatatat cgtttgaaaa ctatatgaat actatttacc taagagcata taagcaaaag
     1081 gttcaggctg taacttatga aatagcctta ccacatcata agttatttat tcagtatttt
     1141 ggttctaaac ttttaaaggg aataacagct tgagattgtg aatcttttag tttacatatt
     1201 attgaaaatt atgcaaagaa tttatggtcg cgattcaaag cttgcatggg gtatgctgaa
     1261 aggttagact atatttcaaa agtgccatgt aaggctttgg ataatcctaa agggaaacat
     1321 cccgacactc cattttggac ttatattgag tttcagaatt ttataaaatc gtttgatttg
     1381 caagattatg aagagctaca aagatttaca actatttggc tatattatat gacaggtgtt
     1441 cgtgtaagtg aaggtttatc attatgatgg gaaaatatcg atttcgaaca aaaattcttg
     1501 aaagttcata caaccttgga aaaggatgaa aatggaaatt ggtatcgtaa agaccaaaca
     1561 aaaacgccag caggagaacg tcttattgag ttagatgatg tgacaatagt agtattggaa
     1621 aattggagaa gaaatcaagt agtcaatact gacacagatt tcattatttc acggtttggt
     1681 gaaccatttt gtaaatcaac gatttgtcga gttattaagc acaaagctca agaagtagga
     1741 gtaccagtga taacgggaaa aggcttgcga catagtcatg cttcctatct tattaatgtt
     1801 ttgaaaaaag acattctgta cgttgcaaag tgtatgggac atgctgataa gtctacaact
     1861 ttgaatacat atagtcactg atttaacgca ttagataaaa cagtatctga agaaataacc
     1921 caaaatgtaa taagttcagg attagattcc attttatacc aaaattcctg ccaaacaact
     1981 aattgatata acgaagctta ttatatcgcc catataacag gatgtgttat tgttacaggg
     2041 tacttgcttt atgagcttgt ttaagctact aaaggtgggt gccactatac ttccttccaa
     2101 atttctgaga atttttgcta taatagtaac tatgaaagat ataatgataa aaaggtgggt
     2161 tatccttttt tttatggtat taacttgttc agggtttggt aaaacagttc tcgcagctga
     2221 ttttcaagtg ggagcagaac atgcgattgt cgtagaggca gactctggga gagttctgta
     2281 tgagaaagac gctaagacgc cagatgctat tgcctctttg accaaattgg tgacggctta
     2341 tctggtttta gataaggtta aatcaggcca gctgcaatta tcagatcaag ttaatctctc
     2401 agattatgcc tttgagttaa caaaagatag gtctttaagt aatgtacctt ttgacaaaaa
     2461 aacttattca gttcaagacc tgttgacagc tactttggta gcaagctcaa atagcgctgc
     2521 tattgcgtta gccgaaaaag tggcgggctc agagcttcat tttgtaaatc aaatgagaga
     2581 acagctatct cattggggga ttacttctgg taaaatcctc aacgcctcag gtctgccaaa
     2641 tgaggtgtta aaggacaatc gttatcctgg ttctgcgttg gaagaagaaa ataccttgag
     2701 tgctcaagat gttgcagttg tgacgatgca tttgttagaa gattttcctg agattttaga
     2761 gatcaccaaa caaacagaag ttaattttgc tggcaattct attaaaagtt ttaatcaact
     2821 cttaccaggt atggctaagg gtagagcagg agtggatggt ttaaagactg ggacaacaga
     2881 cttggcaggt cattgtcttg tggtaacttc tattgaaaat ggtatgcgag tgattaccgt
     2941 tattctaaat gccgatggat ctgataaaaa ccaaaacacg cgatttgagc aagcaaatcg
     3001 tttgttggac tatgtggata gaacttattg tcgtcgtaag attcttaaaa aaggaagtct
     3061 tgtcttggag cgatcgctcc ctattcaaga tggccaagta aaagaactac cggtatctgt
     3121 tgccgaagat gtaaccatca ttttgcaaca gggtgaacaa gtccccaaac ctaaacaatt
     3181 tatgatatca gagacttctt tactagcacc gattactaaa ggtgaagtgg ttgcttattt
     3241 gacgtctcca cgaataacag atcagtcagt gaggtattta aaggaaccta agcgtattcc
     3301 tcttaaagct agccaatctc ttaaaaaagc ctctgactta cagctgtggt ggcgagattt
     3361 tcttgaaaaa agacgttaaa gataagaata aaataatttt aaaacaatca aaaaagacag
     3421 ttatgaaaga attttttcat aactgtcttt ttagtttaca gtgagtatga tttggaatga
     3481 taaaaaaatt gatgttatgg ttccattgtt tgtaaaagat tttccggact catgtttggg
     3541 aggatacgaa tccggttaag agaaagaagt ccattagccg cgctagctat tccttcgtta
     3601 gtggtaacac ctaatttata gttaagtcta gctgcaattt gtaaggtagt gtcattatat
     3661 ctaccagatg gataagcaat ggcaatagtg ttttggttta attgcttatc aagatagtcc
     3721 tttgaatctt tcatttcagt tgtttgaaca tcaggactag cctgctctaa atcaggatga
     3781 ttcactgtat gatcttggaa agacataccg aattgtttca tttctttcat ctgcttaaga
     3841 gtcagattgg cagcgcttcc tatttcggtt agtcctgtaa taacattatt ggtagcttta
     3901 gcatcatatt tttttaagat tgggtaagca acgttgtaga aatcaatcat actgtcatca
     3961 aatgttagcc atacaacttt tttagcaggc aattcattat ttgaaagagc gcggtagacc
     4021 tcttcaggac tcaaaaaata gtagccctcg tctttcattt tttgaagttg ttgatcaaag
     4081 agatctgggt tgacaatcaa attagcattg gccgtctctt cagggaacat tacatgaata
     4141 gcatgataca ttaaaatggg gatcttgaca ggtgtttctt gcttggtcca agttaattta
     4201 gaatgggcct ttttttgaga ttttgaggaa tcgaaatgag tcttcttaag ttttgtcgtt
     4261 tctttttttg ccgtcttttt gtgatgaaaa agacttattt taggtacaaa ttgagagaca
     4321 tctgacaagc cgaatttgtt agacccttgg tataaataga aaatgagggt caaaccaaga
     4381 atgcaggcaa ataatagtat aaggttaatg agagtgaagg cttttctttt ttggcgccgt
     4441 ttatgtctat tgttcattac ttgctccttt ttttgatata agtacctcta ttgtaacatt
     4501 ttttagagaa aagaaggctt gaagtcagcc ttttttcttg ttttttggaa gaacgtgtat
     4561 taaggagtaa aaaaattgtt gtcacagata acactttagg aggtagcaaa gactatgtgg
     4621 ggctggtatt cttatcacgt aaaaaaagca atgaagataa aagtgtagtg tactttagaa
     4681 gttatttgag tgtttggtga ggataataaa aaaaagggta ctactctagg ttatctgctg
     4741 tcactttttt ttatatatgg tatactaaag atagatgtga ggtatgaaat gacttatgaa
     4801 gagactctag aatggattca tgatcactta gttttcggca tcaagccagg cttaaaacgt
     4861 atgctttggg ttcttggaca gttaggaaat ccccaaaaaa atgtcaaggg agtgcatatc
     4921 gttggaacta acggaaaagg ttccacggtc aatcatttac aacacatctt tacgacagct
     4981 ggttatgaag tggggacctt tacctctcct tatattatgg atttcaaaga acgtattagc
     5041 ataaatggtc gaatgatttc agaaaaagat ttggttattg ctgctaatcg tattcgtcct
     5101 ttgacagagc gtctagttca agagactgat tttggagagg taactgaatt tgaagtgatt
     5161 actttgatca tgttcctcta ttttggagac atgcatcctg tagatattgc tattattgaa
     5221 gcaggccttg gcggtcttta tgattcgacc aatgtttttc aagcaatggt tgtggtctgt
     5281 ccctcaattg gtttagatca ccaagctatt ttgggagaga cttacgctga tattgccgcc
     5341 caaaaagctg gtgttttaga agggggcgag acccttgttt ttgccgttga aaacccgtca
     5401 gctcgagagg tttttttaac aaaagctgag caggttggtg cttcaatctg ggaatggcaa
     5461 gagcagtttc aaatggctga gaacgcatct ggctatcgtt ttactagtcc tttaggagtt
     5521 atttcagata ttcacatcgc tatgccagga caccaccaag tgtctaatgc agctttagct
     5581 attatgactt gcttaacctt gaaagaccgc tatcctaggc taacttcaga tcatataagg
     5641 gaaggtctgg caaatagttt gtggctaggc cgtaccgaat tattagcacc aaatctaatg
     5701 attgatgggg ctcataacaa tgaaagcgtt gctgctttgg tagccgtctt aaaaaataac
     5761 tacaatgata aaaagcttca tattttgttt ggtgcgatcg acaccaagcc tattgcggat
     5821 atgttggttg ctcttgagca aataggtgac ttgcaggtta ctggctttca ttaccctaac
     5881 gcctatccat tggaaaaata cccagaacgt tttggtaggg ttgctgattt caaagatttc
     5941 ttggccctgc gtaagcatgc caaagcagat gactttttcg tgattacagg gtcgctatat
     6001 tttatttcag aaattagaca gtattggaaa aaacatattg aaaaaaacgt tcttttaacc
     6061 cattaaaaaa gagaaaatga aaaggaaaag gaaaaaacta atgaaaaggg aaagactaat
     6121 gtctattaat aaggaaaaag cagaagcagc catttatcag tttttagagg ctattggtga
     6181 aaatccaaat cgggaaggtc ttcttgacac gcctaaacgg gttgctaaaa tgtatgcgga
     6241 gatgtttttg ggactgggaa aagatcctaa agaggagttt acggctgtct ttaaagaaca
     6301 acatgaggat gtggtgattg tcaaagacat tagtttttat tctatatgtg aacaccactt
     6361 agttcctttt tatggcaagg ctcatatcgc ttacttgcca agtgatggtc gagtaacagg
     6421 tttgagtaaa ttagcgcgag cagttgaagt agctagtaaa cgacctcaac tccaagagcg
     6481 tttgacctct caaatagcgg atgctttagt tgaagccctc aatcctaaag ggactttagt
     6541 tatggtagaa gcagagcaca tgtgcatgac catgcgaggc atcaaaaagc caggtagtaa
     6601 aaccattacc accactgcca gaggcttata taaggaaagt cgtgctgaga ggcaagaagt
     6661 tatttctcta atgacaaaag attaggagaa cttatgaaaa ttggaaagtt tgtgattgag
     6721 ggcaatgcgg ctatcatggg gattttaaat gtgactccag attctttttc agatgggggg
     6781 tcttacacta ctgtgcaaaa agcattagat cacgttgagc aaatgattgc tgatggggct
     6841 aaaatcatcg acgttggtgg agaatcaaca cgtccaggtt gccaatttgt aagcgctacc
     6901 gatgaaattg accgggtggt tcctgtgatc aaggccatca aagaaaacta tgatattcta
     6961 atcagcattg atacctataa aaccgaaaca gctagagcag ctttagaggc gggtgccgat
     7021 attctcaatg atgtttgggc aggtttgtac gacggtcaaa tgtttgcctt agcagccgag
     7081 tacgatgcgc ctatcatctt gatgcataac caagacgaag aagtttatca agaggtaaca
     7141 caagacgttt gtgattttct aggcaataga gcacaagcag ctcttgatgc tggcgtgcca
     7201 aaaaacaata tttgggttga tccaggattt ggatttgcca aatctgttca acagaatacg
     7261 gagttattaa aaggattgga ccgcgtctgt catttgggct atcctgtctt gtttggtatt
     7321 tcgagaaagc gtgtcgtaga tgccttgtta ggcggaaaca ccaaagctaa agagcgagac
     7381 ggagcgacag cagccttgtc tgcttatgcc cttggaaaag gctgtcagat tgtacgcgta
     7441 cacgatgtca aggctaatca agacattgtg gctgtgttga gccagttgat gtgaggactt
     7501 atggataaaa tcgtattaga aggttgccgt ttttatggct accatggagc ctttaaagaa
     7561 gaacagaccc ttgggcaaat ttttctggtt gatttagaat tgtcagtcga tttgcaagca
     7621 gcttctttgt cagaccaatt aacagatacg gtccattacg ggatggtgtt tgatagtgtc
     7681 cgtcagttag tggaagggga aaagtttatt ttgattgaac gtttagcagg tgcgatttgt
     7741 gaacagcttt ttaacgaatt tccgcctatt gaagccatca aagtggccat taaaaaggaa
     7801 aacccaccga ttgcaggcca ctataaggca gttggtattg aattggagag acagagatga
     7861 ccattgttta tttaagttta ggcaccaata tgggggaccg agcagcttac ttgcaaaaag
     7921 ctcttgaggc tttagctgac ttacctcaaa cacggttgct tgctcaatca tccatttatg
     7981 aaacgactgc ttggggcaag acaggccagg ctgattttct caatatggcc tgtcaattag
     8041 atacacaatt aaccgcggct gatttcttaa aagaaacaca agctattgag caatctcttg
     8101 gtcgtgtgag gcatgaaaaa tggggatcaa gaactattga cattgatatc ttgctatttg
     8161 gagaagagtt ttatgacaca aaggaattga aagtgccaca cccttatatg actgagcgtg
     8221 cttttgtttt aatcccctta ttggaattgc agccagattt aaaattgcct cctaatcata
     8281 agtttttaag ggattatctt gcagctttag atcaatcaga tatcacgctc ttttcagctc
     8341 aacagacaga gttctagact ctgttttcgg agtaatgcct gctataagcc aatccataaa
     8401 gttttgccaa attcatcgtt attttttcta aaatttgata taatagtatc ggctttatgc
     8461 cgattttttt acgcgtataa gaagtgataa aagaaagaag ataactatga taactgaact
     8521 tcatgggatt gatattcgag agaatgaacc cctaaaacat tacacttaca ctaaggtagg
     8581 tggcccagca gatttcttgg cttttcctag aaatcactac gagctatcac gcatcgttgc
     8641 ttatgccaac aaagaaaata tgccttggct tgttttggga aatgccagca atctcattgt
     8701 gcgagatggt ggtattcgtg gctttgtcat catgtttgat aagctaaatg cagtgcactt
     8761 aaatggttat accttagaag ccgaagctgg tgccaatctg attgaaacaa cgaagattgc
     8821 caaattccat agtttaacag ggtttgaatt tgcatgtggc attcctggaa gcattggggg
     8881 tgctgttttt atgaatgcgg gtgcctatgg tggggaaata tcacatattt tcttatccgc
     8941 taaggtgctt acctcaagtg gagagatcaa aaccatttca gctagggata tggcttttgg
     9001 ttaccgtcac tctgccattc aagaaacagg tgacattgtc atttctgcta agtttgctct
     9061 taaaccgggt aattatgata cgatcagcca agaaatgaat cggttaaatc accttcgcca
     9121 actaaaacaa cctttagaat ttccatcttg tggatctgtg tttaagcgcc cgccaggaca
     9181 ctttgcaggt caattaatca tggaagcaaa tcttaaaggg catcggattg gtggtgtgga
     9241 agtttccgaa aaacacgctg gttttatgat taatgtggta gatggcacag ctaaagatta
     9301 tgaagatttg attgcttatg tcattgagac agttgaaaac cattctggtg tcaggcttga
     9361 accagaagtt cggattattg gggaaaacct gtaaccatta ttgataatta aagaaagtac
     9421 cgtggaggat ttatgacaat tgactaagcc aattatcaca tttaacaatg tttcaaaaac
     9481 atttgaggac agtggaacac aggttctaaa gaacattaac tttgaccttg aagaaggaaa
     9541 attttacacc ctgcttggag cttctggctc tggaaaatca actattttaa atattatggc
     9601 gggcctacta gatgccagca gcggagacat ttatttagat ggggaacgta ttaatgattt
     9661 gccgattaat aaacgtgaca ttcataccgt tttccaaaat tatgccctct tccctcatat
     9721 gactgttttt gagaatgttg cctttgcttt gaagctgaag aaagtggaca agaaagagat
     9781 tgctaagcgc gtgaaagaaa ccttgaaaat ggttcaatta gaagggtatg aaaatcgttc
     9841 tatccagaag ttatctggtg gtcaacgtca acgtgttgcc attgcgcgtg cgattatcaa
     9901 tcaaccgcgt gtggtcttac ttgacgaacc actctcagcc cttgatttga aattaagaac
     9961 agagatgcaa tatgaattac gtgaattgca acaacgtcta ggcattacct ttgtttttgt
    10021 tactcacgat caagaagaag ccttggccat gagtgattgg gtttttgtca tgaatgaagg
    10081 tgaaattgtt cagtcgggaa caccagtgga tatttatgat gagccaatta atcattttgt
    10141 tgctaatttt attggagaat ctaatattat taacggtacc atgattgaag actatcttgt
    10201 ctcctttaac gggaaagaat ttgaatctgt ggacggtggg atgcgcccta atgagcctgt
    10261 tgaagtggtt attcgtcctg aagatcttca aattactttg ccagaagaag ggaaattaca
    10321 agttaaggtt gatacccaat tattccgcgg ggttcactac gaaattattg cctatgatga
    10381 attgggtaat gaatggatga ttcattctac ccgcaaagct atcgagggag aagttatcgg
    10441 attagacttt acccctgaag atcttcatat catgcgtctt aatgagactg aagaggaatt
    10501 tgatgcccgt attgaagaat atgtggaaat ggatgagcct gaagatggat tgattaatgc
    10561 cattgaggag gagcgtaatg aagaaaacct ctagtctttt ttcgattcct tacttcttat
    10621 ggattctctt ctttgttgtg gcaccagtca ctctcttgtt ttacaagtcc ttttttgaca
    10681 tagaagggcg cgtgacctta gccaattatg aaaccttttt tagctcttgg acctatttga
    10741 gaatgagtgt gaattctatt ttatacgctg gtattatcac actcgtcacg ctcttgattt
    10801 catatcctac ggctctcttt ttaacgcgcc taaagcacaa gcagttgtgg cttatgctca
    10861 ttattttgcc aacttgggta aacttattgc taaaagccta tgcctttatg ggaatctttg
    10921 gtcaacaagg aggaattaac agctttttaa cctttatggg gattggcccg cagcaaatcc
    10981 ttttcacgga tttctccttc atttttgtag cctcttacat tgagctccct tttatgatgt
    11041 taccgatttt taacgctttg gatgatattg accataatgt cattaatgcc agtcgcgacc
    11101 taggagctag tgaatttcag gccttctcaa aagttatttt tcccctttct ttaaatgggg
    11161 ttagggcagg tgttcagtct gtctttatcc caagtttgag tctctttatg ttaacccgtt
    11221 tgattggtgg aaaccgcgtg attacacttg gtacagccat tgaacaacat tttttgacca
    11281 cccaaaactg gggaatggga tcaaccatag gtgtggtgtt aatcttaacc atggttgcta
    11341 ttatgtggct cacaaaggag aaaagtaaat gaaaaaattt gccaatcttt atttagcgag
    11401 tgtctttgtt ttactctaca ttcctatttt ttatttgatt ttctattctt tcaacaaagg
    11461 tggggatatg aatggtttta caggatttac ccttgagcat taccaaacca tgtttgagga
    11521 tagtcgtctc atgacaatct tactgcaaac ctttgttctt gcttttagta gcgctctact
    11581 agcaacgatt attgggacct ttggagctat ctttatccac catgttagag gtaagtacca
    11641 aaatgccatg ctatcagcca ataatgtctt gatggtatca ccagatgtca tgattggggc
    11701 ttccttttta attcttttta catcattgaa gtttcagctg ggcatgtctt cagttttatt
    11761 aagtcatatt gctttttcga ttcctattgt ggttttgatg gtattgccgc gcttgaaaga
    11821 gatgaatcaa gacatggtca acgccgctta tgatttggga gctaattatt tccaaatgct
    11881 caaagaagtc atgctgccat acttaacacc agggattatt gcaggttatt ttatggcctt
    11941 tacctattcc ttagatgatt ttgcagtgac tttctttttg actggaaatg gttttactac
    12001 tttatctgtt gagatttatt cgcgggctcg tcagggaatt tccttggata tcaatgcttt
    12061 gtcaaccatc gttttctttt tctccatcct cttagtgatc ggttattatt atatgtcaca
    12121 ggacaaggag gaaaaacatg cgtaaacttt attcctttct agcaggagtt ttgggtgtta
    12181 ttgttatttt aacaagcctt tctttcatct tgcagaaaaa atcgggttct ggtagtcaat
    12241 cggataaatt agttatttat aactggggag attacattga tccagctttg ctcaaaaaat
    12301 tcaccaaaga aacgggcatt gaagtgcagt atgaaacttt cgattctaat gaagccatgt
    12361 acactaaaat caagcagggc ggaaccactt acgacattgc tgttcctagt gattacacca
    12421 ttgataaaat gatcaaagaa aacctactca ataagcttga taagtcaaaa ttagttggca
    12481 tggataatat cggtaaagaa tttttaggga aaagctttga cccacaaaac gactattctt
    12541 tgccttattt ctggggaacc gttgggattg tttataatga tcaattagtt gataaggcac
    12601 ctatgcactg ggaagatctg tggcgtccag aatataaaaa tagtattatg ctgattgatg
    12661 gagcgcgtga aatgctaggg gttggtttaa caacttttgg ttatagtgtg aattctaaaa
    12721 atctagagca gttgcaggca gccgagagaa aactgcagca gttgacgccg aatgttaaag
    12781 ctattgtagc agatgagatg aaaggctaca tgattcaagg tgacgctgct attggaatta
    12841 ccttttctgg tgaagccagt gagatgttag atagtaacga acaccttcac tacatcgtgc
    12901 cttcagaagg gtctaacctt tggtttgata atttggtact accaaaaacc atgaaacacg
    12961 aaaaagaagc gtatgctttt ttgaacttta tcaatcgtcc tgaaaatgct gcgcaaaatg
    13021 ctgcatatat tggttatgcg acaccaaata aaaaagctaa ggccttactt ccagatgaga
    13081 taaaaaatga tcctgctttt tatccaacag atgacattat caaaaaattg gaagtttatg
    13141 acaatttagg gtcaagatgg ttggggattt ataatgattt atacctccaa tttaaaatgt
    13201 atcgcaaata agcactcaaa aaggctggta tttctccagc ctttttaatg tatcgacaga
    13261 tgacgtcacc agtacacgcg atagggacga ccaaccttgg taaaaatttg ttggctgttc
    13321 agttgcttat tttcttctaa ataagcaatg tatttacgga cagagacatg ggataagtga
    13381 caagcggaag ctaattcttg gatagtaaaa ggttgatcaa aggctttgat attttctata
    13441 atccactgga aagtggattc agataagcct ttttctaaca attgtttgtt tttagtgtct
    13501 tttttgctag tcaaacattt cagttggtca atttgggcct gctctagttg ctgattggcc
    13561 aaatgttctc gatgcgtaac aaactgttgg atgctttctt gaaagcgttc aaaggtaaag
    13621 ggcttgatga ggtagtcgat aattcctaaa tgaaaaccat ctcggataat attcccatca
    13681 tttgcggctg aaataataat gacttcgcaa ggaatatgct gagcacgcca tttctccaaa
    13741 aattgaatcc cattgccatc tgtaatatga atatccagta aaatgaggtc aattgcgtaa
    13801 tcagtaagta tgctttgaac agctttcata gagtcgctgg atataatgcg atcaaaaaga
    13861 tttagttttt cgaggtaatt gcgatggata aaatccacca taggatcatc ttcaatgatt
    13921 aaaacgttca tgttgccatc ctacataagg tgttgtcact gagaactgta actcggttac
    13981 ggtttgatgt tgatggaatt gggaatttgt atcggtcaag agctgttgaa aataaggtaa
    14041 gtcaaaaata ggctgtaccc gctccaaatc aatccaattg tctgtcaagg tgtaatggct
    14101 gattaaatga ttctgatcat gttgaatact caaaacgagt gatttgggtc tcagtgtggt
    14161 tagaagatta gtatggataa agcggtaaat catcaaacca ttattaagtt gattcttggt
    14221 aacagaatga ggaatctcgc ttaaaacatc aatcttgaga tggacattta attcttggta
    14281 tttctcttgc tctccaatga gaaaactagc tagaagaggc tccttaacaa ggacagataa
    14341 gctagtgaga atttcatttt cgggctccaa aatactgtct aaataaattt tcaattggtc
    14401 ataataagca atatctacta agccataaat aacatgcaat tgattcatga atttatgagt
    14461 ttgggcttgt agagcagaag cataggcggt ggtataggct agctgatcca aggtatcaat
    14521 agccttaact gcttctcgca tgaaaataat gtggcctcgg tgatcgtttt tgacacagat
    14581 gggtgaaatg gctaacaagt agtcttcctc accataccga aagagttgtt catgcccctc
    14641 ttgcaaacta agttttggaa aatcaggaaa gaggtgattg aaactgttgc cagtaggttt
    14701 tcccaactgg cattttcgag cgattaactc ttgtccagcc tgattgcata attgtaaaat
    14761 accagtttta tctaccacaa atacggcggc ttcgatttga tccaacatgg cattacgctc
    14821 ttcaaagagc tggtaaattt cacttggttc taactggtgc agttgacgct tgagtcgaaa
    14881 agaaataaaa ctggtgacca gtaaactaat aagaagacaa aggaataaag acagggtata
    14941 attgcgtttt gacgttaaag ccacatcatt tagggtagtt agtttaatgc caaccgcaat
    15001 agcaccaatc tgtttgtcac cgtcaaacac aggtactaga tagcgaagtg actttcctaa
    15061 agtgcccttg gcggtagaga taaccttttt tcctgctaga acagcctcct catcacctcc
    15121 ttgaaaaggt tttccgatat ttttcgggtt aggacgggtg agtcgaatcc ctttcatgtt
    15181 catcacgacc acataatcaa ggttatagat ggaggcaatg gagttggtat aggctgttgt
    15241 tttagcattt ggttggttgt ttaggagtaa ttctttaatt gcttgatgac ttgctaacat
    15301 ttttccagtt gacgttagca aatgggtttc ctgatttttg atggattggt gcgtatcatg
    15361 tagcataatc ccgtaaaata gggaggttgt gacaacaatc atagatacca aaatcaacga
    15421 taaactggcc cataaacgta gtggtttttt cataaggtct cctctttatt gagtataaca
    15481 cgataagaaa tgacctgcta gcttaaatta attaaataac ttgaaaaaat taaagtagtt
    15541 aaacaactta aataaactat gcttttgaca tgttctcttt tatactagtc ttatcactaa
    15601 aggaggttgt ttcatggaaa caataagcaa gaaaatgcct caaaaagact tgtctgagca
    15661 ttcaaaagca tggcaaaata ggcgaatagg ctccgttccc ttgcctgttt atctggtttt
    15721 agctacctta attttgatca caggctggct ccaacaattg cctgttaata tgctgggtgg
    15781 ctttgctgtt attttaaccc ttggttggct actaggaacc attggagcga ccattccagg
    15841 cctcaaacat tttggtggcc cagctatttt atcactctta gtcccttcga ttttggtttt
    15901 ttttaatctc ctcaacccca atgtcttaga agcgacgaat gtcttgatga agcaagccaa
    15961 ttttctttat ttttatattg cctgtctggt ctgcggtagt attttaggca tgaatcgaaa
    16021 aatcttgatt caaggtcttt ttcggatgat tattcccatg ttacttggaa tggtttgtgc
    16081 aatgggtgta gggactttgg ttggcgtcat tttaggatta gactggcaac ataccctctt
    16141 ttatgttgtg acgccagtcc ttgctggagg aattggtgaa ggtattttac ccttatctct
    16201 tggttacagt gccattacag gagtcggaag tgagcaatta gtggctcaac tgattccagc
    16261 caccattatt gggaatttct ttgctatctt gtgcacggct ttgctcaatc gttttggaga
    16321 gaagcaccca agttattctg gtcaagggca actggttaaa attggtcaca gcgaagacat
    16381 gtctgatgcc ctaaaagaca attctggagc cctagatgtt aaattaatgg gggcaggcgt
    16441 tttaacagct tgttcacttt ttattgctgg tggcttgtta cagcatttaa caggttttcc
    16501 aggaccggtg ttgatgatta ttctggcagc cttcttgaaa tacctgaatg ttattcccca
    16561 agaaacccaa aatggtgcca agcaacttta taaatttatt tccagcaact ttacttttcc
    16621 gttaatggca gggcttggac tgctttatat ccccttaaaa gaggttgtgg caacccttag
    16681 ctggcaatat tttattgtgg tgattagtgt ggtgttaacg gttgtcagtg tcggtttctt
    16741 tgtctctcgc ttccttaaca tgaaccctgt tgaagcagct attatttcag cttgtcaaag
    16801 tggtatgggt ggaacagggg acgtggctat tttgagtacg gctgaccgca tgaatctaat
    16861 gccgtttgcc caagtcgcta ctcgtctagg tggggctatt accgttatca ccatgacagc
    16921 tattttaaga attattttta gttaatccaa accaataaaa aagagaggta tttcctatga
    16981 aaaatcaatt aggtcaactt gcccttgaac aagctaaaac gttcggaggt aaattggaag
    17041 ttcaaccaaa agttgacatt acaaataaac atgatttgag cattgcctat acaccaggtg
    17101 tagctggtgt gtcatctgcc atcgcaaaag ataaaacatt agcatatgac ttaatcacca
    17161 agaaaaatac agtagccgtt atcagtgatg gtactgcggt tcttggtctg ggggatattg
    17221 gccctgaagc agccatgcca gttatggaag gaaaagcagc cctttttaaa gcatttgcag
    17281 gtgtcgatgc tattccgatt gtattagata ccaaagatac tgaagaaatt attagtattg
    17341 tcaaggcgct tgcaccaacc tttggtggta ttaatttaga ggatattagt gcgccccgct
    17401 gttttgaaat tgagcaacgc ttaattaagg aatgtcatat tcctgttttc catgacgatc
    17461 agcatggaac agctattgtg gtgttagcag ccatctttaa tagtttgaaa cttcttcaaa
    17521 aatcccttga tgaggtatcc atcgtggtga acggtggtgg atctgcggga ttatccatta
    17581 cccgtaaatt attagcagca ggtgctacga aagtgacagt agtggataaa tttggcatta
    17641 tcaatgaaca agaggcagat cagttagcac cacaccattt ggatatagcg aaagtcacta
    17701 atcgtgagtt taaatcaggt accttagaag atgctcttga aggagcagat atcttcattg
    17761 gcgtatcagc accaggagtc ttaaaagcag agtggattag taaaatggca gcgcgcccag
    17821 taatttttgc catggccaac cctattccag agatttaccc tgatgaagcc cttgaagcgg
    17881 gggcttacat tgtaggtact ggccgtagtg attttcctaa ccagattaat aatgtccttg
    17941 cctttccagg tatttttagg ggagcattag atgcacgggc aaaaaccatt actgttgaga
    18001 tgcaaattgc tgccgcaaaa ggtattgcca gtcttgttcc aggtgacgct ttgagcacta
    18061 ctaatattat tccagatgcc tttaaagaag gagttgctga gattgtcgct aaaagcgtca
    18121 ggtcggtagt ccttaaatca tagaatatat gaaaaaaaga gaaagttata ctttaaatca
    18181 caaaataagt agaagcatat ttctagcttc tacttatttt gtgattagct aatatcatta
    18241 tcaataataa ctttcaagac atttagtagc atttcagttg tattggcatt aacaagtcca
    18301 gtgtttaagg tgatattttt aatccataat tcatccaggt tgaaatcaac tggtttccca
    18361 tgaaccccaa cgttagcaat atgcccacct actgatataa ttttttggca aatatcaaat
    18421 gtcgcgggat aaccaacaca ctccatggaa atatcgacac cacgtccatt ggttatatcg
    18481 tcaatgatag ccttgacttc ttcactactt cctgaacaaa tcgtgtgtgt ggctccaaac
    18541 gttttagctg cttcaaggcg attttgagat aagtcgacca taataatatt ggctggagag
    18601 aaaagttgaa cggttagtag ggctgcgagt cctactggac cggcaccaac aatacaaaca
    18661 ttatctccag gcttgacatg tgatggcaag acaccaattt catatgaagt tggtaaaata
    18721 tcagaaagca tgaccagtgc ctcatcatca atcgtatcag gagcatgata gagactacca
    18781 tctgcatgtg ggatatggac atattctgct tgtgttccat taattaaatg gccaagaatc
    18841 caaccgccat cttcacaatg tgacgaaagg cctcgtttac aataataaca ggtatgacat
    18901 gatgtgacgc aagaaataat cactttatct ccgattttaa agttagtgac cgcatcaccg
    18961 atctccttaa caattccaat tccttcagga ccaaggattg ttccttcttt acatgcggga
    19021 acatccccac ctaagatatg caaatcagtt ccacaaatag agtggttttt accaactgga
    19081 cgatatgtag gctttataat gactggtttt ggtttgtcta tgagctgtag atttccggtt
    19141 gaaaggtaag ttgctgcttt catatttaac tctttataag gttgagaggt aatcaaccaa
    19201 ttcattttta cacagatatt ataatagttt aagaaagggt tttcaatatt ttttaaatcg
    19261 attatgataa ttttttagat catctttgtt gaataacttc atccttattc agaacttaaa
    19321 gggcactatt ataagaattt gaaaccgctt tacttaaacc gtgtattttt gtacaataat
    19381 gattaaggag gtttttagat gaaaaaagaa tttaccagta tcctgtttac agttagtttt
    19441 tgtggaatca tagcattgcc tgtcgaagct agtggcccta aagttcctta tacacaagaa
    19501 ggtataacgg ccatttcaaa tcaggcaaca gtaaaattga tttctattgc tgatatcgcg
    19561 agcagtttag aaggacaaaa acctataacg gttagttttg atatcgatga tactttgtta
    19621 tttactagcc aatattttca gtacggcaaa gaatatataa ctccaggctc atttgatttt
    19681 ttgcataagc aaaaattttg ggacttagtc gctaaacggg gagatcagga ttctattcca
    19741 aaagagtatg caaagcaact aatcgcaatg catcaaaaac gaggagataa aattgtcttt
    19801 attactggcc gaactcgagg atcgatgtat aaaaaaggtg agattgataa aacagctaag
    19861 tcactggcta aagattttaa gttagacaag ccaatagcta tcaattatac aggagataaa
    19921 gctgtgaagc cgtatcagta tgataaaaca tattatataa agaaaaacgg ctcacaaatt
    19981 cattacggag atagtgatga agatataaat gctgccaaag aggcaggtgc aagacctatt
    20041 cgcattttaa gagctcctaa ttcaaccaat cttcctttgc caaaagctgg aggatacgga
    20101 gaagaggttt tagaaaattc ggcttattag tcaaatattt gtgtaagaaa ggaagtaaca
    20161 gtaatttctt tcatcacccc actgagtaac caatagccca gtgggttttt tgggttacag
    20221 aaatcagttc taaaaaagaa tcactggtaa cttaaggttg tctttgatac aatggtaaca
    20281 gtgtatttaa aagaaagaga ggataacatg gaaaaccata aaaatgaatt caccttttcc
    20341 aacaaatcaa ttattgccta tgtttggcgt ggtgttatgg ttggtattat tgctggtgtt
    20401 attgttagtt tattccggtt gttaattgaa gtgacagctg actgggtgat tgaatggtac
    20461 cgatacgctc atatcaattc gctgttgcta cttcctatac taagtgtcag tttgttagct
    20521 gttctttttg taggtttttt agtaaagtct gattctgata taaaaggatc agggatcccc
    20581 catgtcgaag gagaactaaa ggggctaatg tccccagatt ggtggagtgt cctttggaaa
    20641 aaatttttag gaggaatcat ggctatttct atgggattta tgctcggtcg tgagggtcct
    20701 tcgattcagt taggtgctat gtcagccaaa ggattagcta agtttttaaa atcgagtcgt
    20761 ttggaaaagc gagtgctaat tgccagcgga gcagcagcag ggctatcagc agcttttaat
    20821 gctccaatag caggcctctt gtttgttgtt gaagaaattt accatcattt ttcgcgttta
    20881 atttggatta cagcgctagt ggctagctta gtggctaatt ttatttctct gaatattttt
    20941 ggattgaaac cagtcttagc aatgtcagag gcgatgcctt ttttagggct aaatcaatat
    21001 tggttgttgc ttttacttgg attatttcta ggctgcttag gctatttata tgagatagtt
    21061 atccttaatt ttaataaact ttatgttatt ttagggaatt ggttacatct gccagcttat
    21121 ttttacggta ttattatggt ttttctaatt ttaccgattg gctattattt accacagtta
    21181 cttggaggag ggcatggttt aatactttct ttatccaatc aacaattgcc tttgatgact
    21241 atcttctttt attttattat tcgatttatt gttagtatgt ttagttatgg aagtggctta
    21301 cctggaggta tttttttacc cattttaact ttaggtgctt tggcaggcct attatttggg
    21361 caaatagctt ctcaattagg tttattgaac caatcttttc tttctctttt tttgatatta
    21421 gggatggcag ggtattttgc ggcaatttca aaagcaccac tgacaggcat gattttggta
    21481 actgaaatgg taggtgattt gaaaccctta atggccatag ctgtagttac ttttgtctcc
    21541 tatttagtga tggatttgtt aaatggacag ccaatttacg aagctatgct agataagatg
    21601 gcaatgaagc atccaactaa tcttgttgaa ccaacattaa ttgaattgac tgttggtgat
    21661 aagattgcag ggaaatatgt taaagagtta aagcttccag aaaatgtctt aattacaacc
    21721 caaattcacc atcaaaaatc tcaagttgtg tcaggtaaca cgcgcctttt gtcaggagca
    21781 actatctttt tggtcgttaa tgaagctgat acaggttttg tccgtgaggt attgatgtag
    21841 gtattaattt atcacaattc ggaaagttcg agcttgtctt ttagactaag tcttcgaacg
    21901 ttttactgct agagtttcat ctgaaccaaa gtgaaacgct tttgatgaag tcaagtaggc
    21961 tttttgttat actaacaata aagataagct aaggaggctg agatgttaga aattgtttca
    22021 gaagagctac gtcactatca agaacaaaaa ttgattgagt accgtaacaa aaatcagtta
    22081 gccccaaaag gaggcattgt atttgcaggt gattctctca ttgaattttt tcctttaaaa
    22141 aaagcttttg gttcatgttt gcctattatt aatcgaggta ttgctgggat agatagtcag
    22201 tggttattgc gccatttttc tgtccaaatt actgatttag agcctaaaca tattttttta
    22261 ttaattggtt gtaatgatat cgggcttgga tatgataaat gtcatattgt caagactatt
    22321 gtagagctta ttagccagat tcgtagtcat tgtgtctaca gtcagattta tctcttatct
    22381 ttattaccag tttccaacaa tccccgatat cagaagactg tcaaaataag aacaaatgct
    22441 atgattgatg ctattaataa agatttagca atgattccaa ctatagaatt tattaatctt
    22501 aatacttgtc ttaaagacga aaaaggtggt ttatcagatg agaatactct agatggttta
    22561 catcttaatt ttccagctta tgctaaacta gctgaaatta ttcaagctta tatctagcaa
    22621 ataaatgtac taatagcgat gaggcaagaa gtcagatagc agatggttca tcaatcaatt
    22681 aagaaaaaag ttatcaagat accattaaaa atagttgctc aaagttcttt caaatcaaaa
    22741 gattatgata tcgctatgtt gagttttagt gtataatgat aattaaagga gttaccatga
    22801 aatttgaaaa aaaacaagtc ttctatttag tccttacgtt tatcttatgc tatgggatcc
    22861 tagcaaactg gcgaaatgga acggctatcg tcacgactat ttacaaaaca agccttcctt
    22921 ttttttatgg agcagcaggt gcctatattg ttaatattgt aatgagcgct tacgaaaagg
    22981 tatatgttta tatctttaaa gactggtcac atgtcttaaa agtaaaacgg ggtatttgtt
    23041 tactgttagc gtatttgaca ttttttattt taattacttg gatt
//
LOCUS       DATUYR010000025        21988 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.025, whole genome shotgun sequence.
ACCESSION   DATUYR010000025 DATUYR010000000
VERSION     DATUYR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.025"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            51..416
                     /locus_tag="VCI33_000926"
     CDS             51..416
                     /locus_tag="VCI33_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP2831859.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            453..1472
                     /locus_tag="VCI33_000927"
     CDS             453..1472
                     /locus_tag="VCI33_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEP2831860.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            1926..2246
                     /locus_tag="VCI33_000928"
     CDS             1926..2246
                     /locus_tag="VCI33_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831861.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            2236..4236
                     /locus_tag="VCI33_000929"
     CDS             2236..4236
                     /locus_tag="VCI33_000929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEP2831862.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEERHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKKHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVALWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            4238..4717
                     /locus_tag="VCI33_000930"
     CDS             4238..4717
                     /locus_tag="VCI33_000930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEP2831863.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            4785..5369
                     /locus_tag="VCI33_000931"
     CDS             4785..5369
                     /locus_tag="VCI33_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEP2831864.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            5385..6383
                     /locus_tag="VCI33_000932"
     CDS             5385..6383
                     /locus_tag="VCI33_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEP2831865.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTETQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            6380..6700
                     /locus_tag="VCI33_000933"
     CDS             6380..6700
                     /locus_tag="VCI33_000933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEP2831866.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            6901..8676
                     /locus_tag="VCI33_000934"
     CDS             6901..8676
                     /locus_tag="VCI33_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEP2831867.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKKEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     QSNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            8677..10092
                     /locus_tag="VCI33_000935"
     CDS             8677..10092
                     /locus_tag="VCI33_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEP2831868.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGHPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGLYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            10137..10763
                     /locus_tag="VCI33_000936"
     CDS             10137..10763
                     /locus_tag="VCI33_000936"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEP2831869.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(10883..12145)
                     /locus_tag="VCI33_000937"
     CDS             complement(10883..12145)
                     /locus_tag="VCI33_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEP2831870.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDNM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSIKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTRA"
     gene            complement(12158..13036)
                     /locus_tag="VCI33_000938"
     CDS             complement(12158..13036)
                     /locus_tag="VCI33_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831871.1"
                     /translation="MIRKYDRTSTKNKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            13474..14766
                     /locus_tag="VCI33_000939"
     CDS             13474..14766
                     /locus_tag="VCI33_000939"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEP2831872.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            15093..16136
                     /locus_tag="VCI33_000940"
     CDS             15093..16136
                     /locus_tag="VCI33_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP2831873.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSAKQITDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIEAAK
                     KAIIEGTITVPEN"
     gene            16309..16848
                     /gene="nusG"
                     /locus_tag="VCI33_000941"
     CDS             16309..16848
                     /gene="nusG"
                     /locus_tag="VCI33_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEP2831874.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            17219..18574
                     /gene="nga"
                     /locus_tag="VCI33_000942"
     CDS             17219..18574
                     /gene="nga"
                     /locus_tag="VCI33_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEP2831875.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFASPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     RYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            18579..19064
                     /locus_tag="VCI33_000943"
     CDS             18579..19064
                     /locus_tag="VCI33_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEP2831876.1"
                     /translation="MYKVPKGLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILENGVYPAFLKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            19088..20803
                     /gene="slo"
                     /locus_tag="VCI33_000944"
     CDS             19088..20803
                     /gene="slo"
                     /locus_tag="VCI33_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEP2831877.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTIEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDMTYPAALQLADKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRT
                     EYVETTSTEYTSGKINLSHRGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            21058..21444
                     /locus_tag="VCI33_000945"
     CDS             21058..21444
                     /locus_tag="VCI33_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831878.1"
                     /translation="MKQQSYQPLRLVYLLVALFAAMLLIARPVMADGEQKDPWELGYN
                     QGLTKGKEDGESAKWTDLTPTVPTDPTIPQPPTVIEETNKGIYKEGYKDGYKEGYNKG
                     FGGSYPLLIPVQFIWGLILHWWGKLS"
     gene            complement(21760..21972)
                     /locus_tag="VCI33_000946"
     CDS             complement(21760..21972)
                     /locus_tag="VCI33_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831879.1"
                     /translation="MKKKLSLFMIATAAVVGLSLATPENQTVSANETTTTQACADGTN
                     CCGYNFRGYCGPITWLYYCIRDGKIY"
BASE COUNT         6846 a         3908 c         4683 g         6551 t
ORIGIN      
        1 atgcggtttc ctgtaaaatt taactatatt atcaatgagg aggttcgtat atgaagagct
       61 acccagaacc aatgggtcct tattcaactt atacgattga aggacatttt ctatacaccg
      121 caggccaatt accccttaat ccagtgacag gtcaattgtc agatggtttt gaggcacagt
      181 gtcgccaggt ttttgttaat ttacaatcta ttttggcaga gcaaaagttg gacttgaatc
      241 atatctataa gctaaatgtg tatttaacag atgtgaccaa tgtggaaata ttaaatcatg
      301 tcatgactga tttgtttgaa gagccctacc ctgttcggac agcggttcaa gtgtcggcgc
      361 ttcctttgca agctttgatt gaagtcgaag ctgttgccag agtgaagcag ttatagtgtc
      421 ttatgtaatg agatagattg gtggaataga ttatggatat tattattgga acaagtcttt
      481 tgattcttgt tttagctatt tttagcttgt ttaattataa ggctcctcat ggtgccaagg
      541 caatgggagc tttggcttcg gcagcttgtg cctctttttt agttgaagcc tttcaagatt
      601 ctttttttgg taaggtttta ggctttcagt ttctaagtga agtaggcgga gcaaatggct
      661 cattatcagg ggtagctgct gctatcttgg ttgccattgc aattggtgtg tctccaggtt
      721 atgccgtttt gattggtttg tcagtttcag gaacaggtat tatcccaggt tttgtggctg
      781 ggtatgtggt atctttttta atcaagtgga tggaaaaaaa tattcctggt gggcttgatt
      841 taatctcgat tattattgtc ggtgcaccat taactcgttt cttggcgcag ttaattacgc
      901 cagtgatcaa tagtacttta ttgactattg gagacatcct gacctcaagt gctaatagca
      961 accctattat catgggaatg atactaggcg gtacgattgt tgttgtagca acagctccgc
     1021 tgtcatcaat ggctttgaca gccatgcttg gtctgacagg tattccgatg gctataggtg
     1081 ccttatctgt ctttggctct tcttttatga atggtgttct tttttaccga ttaaaattgg
     1141 gtgaaagaaa agacaatatt gcttttgcta ttgaaccact aacgcaggca gatgtgacgt
     1201 cagccaatcc cattccgatt tatgtgacta attttgttgg tggtgctgca tgtggtgttt
     1261 tgatcgccct aatgaaattg gttaatgata cgccagggac agctactccg attgccggtt
     1321 ttgcggtgat gtttgcctat aatcctgtcg ctaaagtgtt gataacagcc cttggatgta
     1381 ttattatcag cttaattgtg ggttatattg gaggttctgt ctttaaaaat taccgtttag
     1441 taacaaaaca agaattacaa gcgagaaact aataaacatc tttttgacag gacttttaac
     1501 aagatagcag ttatactgtt atcacattgg cttggctatt ggctggtaaa ggttccttac
     1561 cgtcaactag atcaaggtta ctaaatgcct aaagaagtat aagataacac acgaaagaca
     1621 gcaatacaaa gctttagagt tctgtgtcat cttactgctt tgacaggttt ttagctaacc
     1681 tttttctgaa ctaagccatt gtttttatgg atagaattga aagcgcttaa cttaaagatg
     1741 ctgttcgctt aatcaagcaa ttatcctatc tcaaaaaagc tgttagtaga taggttagag
     1801 atttagctct agcttatagt cgctggttga ttaaccccca aaagtagcct ttcttgtagc
     1861 tttgttttta agtacctagt gagtcaaaaa attagcaata gataaagata aggagttgat
     1921 atgtgatgaa taccctattg tcactaatga atagtatcga acaagaggct agaacaattt
     1981 atgacagtta cgatgctaag aaaaaagctc tagctgacaa aaaagaagaa gagttaaagc
     2041 agctgtttct tgagtacgaa caagaaactc agctactatt agaagctaaa cgtgaggtat
     2101 tttttgaagc taatcgtcaa gaacaaaaag cttcaaaaaa gaggttagcg acgcaacagc
     2161 tagctcttca tgccatttta gaagagcata agctaaccgt tgttagggag ttagtggata
     2221 aggtggtgga gacatatggc cattagtcaa atgaaaaagc tggcaatggt ttttgaaaaa
     2281 gattatcttg atcttgtttt aaaaacactt caacaatctc aattagttga agttcgtgac
     2341 atgaagcaac taaaacattg gcaagatgct tttaacaaag gcaacgtcaa gttgccacaa
     2401 atcgtccaat acgatctgac gcatcaaaaa cctctactag atgatgaagc tttacaatac
     2461 cttttacaaa gccaacaaga gttagaaaac ggccttgcta gcttatcagc gtttttacca
     2521 ccaataggaa agttaacagc cttgagacaa aaaacgccat ctctttcgtt taaacagttc
     2581 gaagaacgcc atcgacaaca agcagctcaa acggctttga aaatgatgtc acaaaagata
     2641 gagcgactag agcagctgca aaacaaaata gaccagctaa cagaatactg tcaggagtta
     2701 gaaaagtggc gttcattgac tgttttgcct caggacttag cgcaatttca ttttttgtcg
     2761 gcaagagttg gaacgattcc tagtacagca aatgatcatt tttatcatca gttaaagaag
     2821 cacaaaggtc tttttattga ggaagtatac catacagaat ttgagtacgg ccttgtctta
     2881 ttttggcaag cacaagatac gattcattta cagaaatatc agtttaaacc cttattatat
     2941 aaagaacagc tactgccatc agaacaactt cgtataaaca aagagttact aacgaattgg
     3001 ctagctgaaa aagatagtct actaaaagag cttaggcagt ctcaaaagat actggcacag
     3061 ttacaggttg aaatagacta tgtccttagt cagtatcaac ggcagcaaac taaaaagcaa
     3121 ttactcggta caagacatct aatagcctta gaagggtgga tagaagctga ttcggttaac
     3181 caattaaaag ggctaatgac taagacacta ggtgatatgt tttatctgga tagttacgat
     3241 gtcacgccag acgattggga agatgttccc attaagttac gtaatcaccg ctacattgca
     3301 ccgtttgaat tggttacaga aatgtacgct ttgccaaaat atcaagaaaa agatccgaca
     3361 ccttttttag ctcctttata ccttactttt tttggcatga tggtagctga tttgggctat
     3421 ggtttgttgc tatatgctgt gacactagct gcattggtct tttttaatct ccaaaaaacc
     3481 agtaaacggt tagtgacctt ctttaatatt ttagctattt cagtagccct atgggggctt
     3541 atttacggtt ctttctttgg ttttgactta cctgtggcat tattatcaac taaaacggat
     3601 gtgattacta ttttggttgt ttctttgcta tttgggtttg ttacacttat atttgggctt
     3661 ttgttaggag catggcaaca agtaaggatg aaagcatatg ctacggccta tacgtcaagt
     3721 ctagcttgga cttttatttt attaggcctg ctgttattta ttcttggtaa gaatgtatct
     3781 ggccttgctt acttatcggt tattggtaag tggcttgcat tgggcaatgc ctttgggatt
     3841 ttggttgttt ctttactaaa aagcaaaagc cttttaggtc taggtagtgg attatataat
     3901 ttatatggca ttagctctta cttaagtgat ttggttagct ttactcgtct aatggcatta
     3961 gggctttctg gagctagtat tggagcggct tttaacatga ttgttgggat ttttccacca
     4021 gtcacgcgct ttacagtagg tatatttatt tttattcttt tgcatgcgat caatattttc
     4081 ttatcaatgc tttcaggata tgtgcatggt gctcgtctta ttttcgtaga gttttttgga
     4141 aaattttatg aaggtggagg aaaagctttc aatcctttaa agttagctga taattatgtc
     4201 aatgtcaatg aagaaacaga tttggaggac aattaacatg gaacatttag cacattattt
     4261 tacagcacat ggtggcgttt tcttcgcagc cttaggaatt gttttagccg tagctcttag
     4321 tggcatgggc tcagcttatg gtgtcggtaa aggtggtcaa gcggcagctg cgctcttaaa
     4381 agaagaaccc gaaaaattta cgtctgcgct tatcttgcaa ctgttacccg gtagtcaagg
     4441 aatctacggc tttgctattg gtattttaat ttggatgaaa ctgacaccag aactttcagt
     4501 taaccaagga ttagcttact ttttggtatc tcttccaatt gctattgttg gttatttttc
     4561 ggccaagcac caaggcaatg tttctgtagc tggtatgcaa atcttggcta agcgccctaa
     4621 agattttatg aagggtgtca ttttagcagc tatggttgaa acctatgcta ttcttgcctt
     4681 tgtcgtctcc tttattctct taaataaaat tggataaggt atggtcctta tctagatttt
     4741 aaaaaacggt gacaccacta acactatcaa aagggggaaa ttaggtgaat gatataacac
     4801 agttaagaca aaacgttctt gaaaaagcac atcaagaagg tcaacagtgc ctaaagatag
     4861 cgacagatag tcttgatact gactttaaag agcgtcaaca gcaaggacta cacgatttaa
     4921 aagcaaagcg tcaaaaagag ttaaaagctc ttgaacagca atttcaggtt gcgcagcagc
     4981 aactcaaaaa tcaagaaaga caagctctac tagcattaaa acaagacagt ataaaggagc
     5041 tatttgaagc ttctttggag aagatgacga acttttctaa ggaagaagaa ttggcttttt
     5101 tgaagcaagt cttgtctaaa tatccagaac agccattgca agttactttt ggcgaaaaaa
     5161 cagggcaaaa gtttagttct tatgattgtg ctgagctgag attagcgttt cctcaattaa
     5221 gctataatca agagctaatt cctcaggaag caggattttt agtcagttta gaccaagtcg
     5281 atgacaatta tttataccgt taccttttag aatcagttct taaagaagaa agctcccgca
     5341 tcattgatat gcttttttca gagatatagg aggtttgact agcgatgcca acatttctag
     5401 aattaaacac tactatcagt gtcaaagaaa aagagttatt gactaaagag caatttgata
     5461 agctattgca ggctcccaat acaacaacct tagctcgact gttgcaccag tcagtctatc
     5521 acctaactgt tgacgatctc aacgatttgg atcggctaga atctatctta atggctgagc
     5581 taaccaagac ctatcgctgg gcttttgcag aaacccctca acctgacata gtacaattat
     5641 ttaccttgcg ttacacctat cataatgtca aagtgttgtt aaaagctaaa gctagtcaag
     5701 cagatctgag tcacttatta ctaccaattg gtgacaagcc tttggtggct ttggaacatt
     5761 tgattaggac gatgacttct gatgagtttc caaaagaggt ggtaacagaa acccagtcca
     5821 tttgggccga atatcaagat tatcaagaca tacgcgtctt agaaattggt accgacttag
     5881 cttattttaa ggcattgaaa caaatagcac aaagattaga ggatcctgtt ttccaacaag
     5941 ctgttcttat tgtgattgat ttgtataatc tgattaccgt cagacgggcc aaatcgcaaa
     6001 ataaaccaat aagtttcatg atgcagttat tatctgatga agcaagtaga ccaagtaaaa
     6061 catttatcac acttgaagat gataaggatc tgatgacgtg gtttgaaaat gtaacacctg
     6121 atagctatat gacagcccta aaaccttatt cagagaaact tcgccaaggc actcttcaaa
     6181 caactgagtt agaatacctt gttgatgaat gcctttacca tttatttgcg aaggccaaat
     6241 atcaagtcga tggaccttat gtgcttgctc gttttttgct cgctaaatcg tttgaagtaa
     6301 aaaatctacg gcttttagcg gctgctttag ctaatgattt acccaaagaa cgtgtcatag
     6361 aaaggatgag accaatagca tgaccaaaac attgtataaa gtcggggtaa ttggtaacag
     6421 agatgttatt ttaccttttc agatgattgg gtttcaaact tttccagtaa taaaaccaca
     6481 agacgctatt aaccagttgc gtcaactagc aatggaagac tttggtatta tttatatcac
     6541 agaagatatt gctgcagcta ttccagaagc cctcactcac tatgacaatc aagttctacc
     6601 agcagtcata ccattaccaa ctcatcaagg agcgcaaggg ataggcctgt cacgcataca
     6661 agccatggtt gaaaaggctg tcggtcaaaa tattttataa cactaagacg ttaggcaaag
     6721 aggacttata ctctgtttga ggcttatgag tagctaactg atgaaatgta aggacaggaa
     6781 aattggtgaa gttagcagct gaacgcatag gatagtttgc tgactattca aaatagtcac
     6841 attaaacctt gacagtatag attagttagt atgatttgga aaaaataaag gaggaaaaag
     6901 gtgaaccaag gaaaaataat aaccgtttcg ggacctcttg ttgtggcttc tgggatgcaa
     6961 gaagctaata ttcaagatat ttgtcgtgtg ggacatcttg gcttagtcgg agaaattatt
     7021 gaaatgcgcc gcgatcaagc gtctattcag gtttatgagg aaacatcagg gatcggtcca
     7081 ggagaaccag tagtgactac tggttgtcct ttgtcggtcg agttaggccc aggcctgatt
     7141 tcagaaatgt ttgacggtat tcagcgaccg cttgatcgtt ttcaaaaagc aacggacagc
     7201 gactttttaa tccgtggtgt ggctatccca agtcttgatc gaaaggctaa gtgggcattt
     7261 attcccaagc taagtgttgg tcaagaagta gttgcaggtg atattttagg aactgtgcaa
     7321 gaaacagctg tcattgagca tcgtatcatg gttccttata aagtttcagg gaccttggtg
     7381 gctattcatg caggtgactt cacagtaaca gatacagttt atgaaattaa gaaggaagac
     7441 ggttccattt accaaggtag cctcatgcag acttggccag ttcgtcaaag tcgccctgtt
     7501 gctcaaaagc ttatcccagt cgaacctttg gttacaggtc aacgggttat tgacaccttt
     7561 ttccctgtta caaaaggtgg tgccgctgct gttcctggac catttggggc aggaaaaaca
     7621 gttgtgcagc atcaaatagc taaatttgcc aacgttgata ttgttattta tgtcggttgt
     7681 ggggaacgcg gcaacgagat gaccgacgtt ttgaatgagt ttccagagtt aattgaccca
     7741 aatacaggcc agtccattat ggagcgcacg gtgttaattg caaacacctc taatatgcca
     7801 gtagcagcgc gtgaagcgtc gatttacaca ggcattacca ttgccgaata tttccgtgat
     7861 atgggctatt ctgtggctat catggcagac tcgacatcac gttgggcaga agctctgcgc
     7921 gagatgtcag gacgcctaca agaaatgcct ggtgatgaag gctacccggc ttacttaggg
     7981 agtcgtattg ccgaatatta tgaacgggct ggtcgtgttc ggaccttggg aagtcaagaa
     8041 cgtgagggaa ccattacagc catcggcgcg gtttctcctc ctggagggga tatttcagag
     8101 cctgtcactc aaaacaccct tcggattgtc aaagttttct gggggctcga cgcgcctctt
     8161 gcgcaacggc gtcacttccc agcgattaac tggctgacgt cttattcatt gtatcaagat
     8221 gatgtaggaa gctatattga ccgtaaacag caatctaatt ggtccaacaa ggtaactcgt
     8281 gccatggcta ttttgcagcg tgaagccagt ctagaagaaa ttgtacgctt ggtggggctt
     8341 gattcactgt ctgaacaaga tcgtttgacc atggctgttg cccggcaaat tcgggaggat
     8401 tatctccagc aaaatgcctt tgattcggtg gataccttta cttcctttcc gaaacaagag
     8461 gccatgctaa ccaatatttt gacctttaat gaggaagcca gcaaagccct ttctttggga
     8521 gcttatttta atgagattat ggaaggcact gctcaggtac gcgatcgcat cgcacgcagc
     8581 aaatttatcc cagaagaaaa cttagagcag attaaagggc ttactcagaa ggttaccaaa
     8641 gagattcacc acgttttagc aaagggagga atttagatga gcgttctcaa agaatatcgg
     8701 acggttagcg aagtggttgg ccctttgatg attgttgatc aagttgcagg agtgcactac
     8761 aacgaattgg tagatattac tttgcataat ggagaaaggc gtaaaggtca agtcttagaa
     8821 gtccaaggtg acaaggctat ggtacagcta tttgaaggat caactggcat caacctagcc
     8881 aaaaccaagg ttcgatttac agggcatcct ttggaattag ccgtatcaga agatatggta
     8941 ggacgcatct ttgatggaat ggggcatccc atagacggcg gtccagaact tattcctgaa
     9001 aaataccttg atatcgatgg tcaagcgatc aatcctgtcg ctcgtgatta cccagacgaa
     9061 tttatccaga caggtatctc agctattgat cacctcaata ccctcgttcg tggtcaaaag
     9121 ttaccagttt tttcgggctc agggttacct cataatgaat tggccgcaca aattgctcgt
     9181 caggcaactg ttctaaactc tgatgataac tttgcggttg tgtttgcagc tatgggtatt
     9241 acttttgaag aggcagaatt ctttatgaat gacttgcgtg aaacgggtgc cattgatcga
     9301 tctgttttat ttatcaattt agctaatgac cctgctattg aacgcattgc aacaccacgt
     9361 atagctctga caacagccga atacttggct tatgaaaaag gtatgcatgt tttagtcatc
     9421 atgacagata tgacaaatta ttgtgaagcc ttacgagaag tatcagccgc tcgcagagaa
     9481 gttccaggca gacgaggtta tcctggctat ctctatacta atctctccac cttatatgaa
     9541 cgggctggtc gcttgatcgg gaaaaaaggg tcagtgactc aaattcctat tttaaccatg
     9601 ccagaagatg acattaccca ccctatcccc gatttgacag gttatattac cgaaggtcag
     9661 attattttgt cgcaagaact atataaaaat ggttttagac cgcctattaa tgtcttgcca
     9721 tcgctatctc gcctaaaaga taaaggctca ggtgaaggta agactcgaca agaccatgct
     9781 gctacaatga atcagctctt tgcagcctat gcccaaggaa aacaagctaa ggaattggct
     9841 gttgtactcg gtgaatcagc cctttctgaa acggataaac tatatgtggc ctttaccaac
     9901 cggtttgaag aagaatacat taaccaagga ttgtacacca accgtagcat tgaagaaagt
     9961 cttgacttgg gttgggaatt gttgtcgatt ttgccacgta ccgaattaaa acgcattaaa
    10021 gatgatatgt tagatcgtta tttgcctaaa gcagatacca cgatgacaaa ggtttttgtt
    10081 gcaaatgatt gagttgacca tgcaatttca agtccttgac gaaaggagtt atatcaatgg
    10141 cacgattaaa tgttaaacca acacgcatgg aacttagtaa cttgaaaaat cgcttgaaga
    10201 cagctacaag aggtcataaa ttattaaagg acaagcggga tgagttgatg cgccgcttcg
    10261 ttgatttaat tcgtgaaaac aatgagcttc gtcaaactat cgaaaaagaa cttgctgcca
    10321 atatgaagga gtttgtcttg gcaaaagcat ctgaaaatag tctaatggta gaagaactct
    10381 ttgctgttcc agtccatgaa gtgaccttat ggattgatat tgaaaatatc atgagtgtca
    10441 atgtgcctaa attccatgtc cagtctaata cggcaagaga gcaagaacaa ggtgagtttg
    10501 cttatagtta cctcagctca aatagtgaga tggacaatac tatccaaaag acaaaagaac
    10561 tattagaaaa gctcttacgg ttagctgaag ttgaaaaaac ttgtcaacta atggctgatg
    10621 acattgaaaa gactagacgt cgcgtcaatg gcttagagta ttctattatc ccgcaactgg
    10681 aagagacgat tcactatatt gagttgaaat tagaagaggc cgagcgtgct agccttgttc
    10741 gtattatgaa aataacatca taaaagataa gtgttatatc aaacaaggcg attattcgtt
    10801 aagtagtaaa aaagcaagcc atcaaaggga ttaacctaac tgatgacttg ctttttaagt
    10861 agcgtgacag gtgatgttta gcttatgcgc gagttgtttt atcaattgtt ttttgagttt
    10921 ccttgccttc actaagaagt gcttgcacaa tattttggtc acgtagtttg acagaatcgt
    10981 taatggtttc ggcagatctg ataatagcac tttctagttg tgctcgttct ttgcgtccat
    11041 gatcaatcgc cgcaatgatc ccattatttt gtgctactaa actttctgcc agacttgtca
    11101 cagattttat agacaaagta gggttttgtg ctgattgttc caatgctggg atggcttctt
    11161 tacttgtttc tgctagcatt tggagggcag cattattagc attaataatg gcatcagcag
    11221 tcattccaga tttaaccgat tgttgcatca tgcctaactg agcaatagat agtttcatcg
    11281 ttggaatggt attgcggcgt aacatgccta gtttttgacg catgtcagaa gagaccttga
    11341 ctagattacg catttgcgga gttgtcgccc aagcaacata gaggcgactc aagtattcgg
    11401 tgtgttgttg ttcgagagtg ttgatgacct cggtagttct tgccaaaaga tcagctttta
    11461 tttgatagtc tggtgtagca ctgtctttgg tttttaggtc tttttgtaaa gccgcagcgc
    11521 gctgactagc ttctttttga ctagcttcga taaaagcaat gacgcctacc aaatgttcaa
    11581 tggacttagt attatcttca attaacaatt ctgctgagac aatattgcga gctaaggtat
    11641 cttcttgctt gacaaccgct gcagccatat tatccatttt ttgctcaatg ttttgagaat
    11701 caaaataaaa ctcttgcagg gtatcacggc tttgtttaaa gagtttttga agaaagtttg
    11761 gctttttatc aaggtccaca ggtgtggcat ccttgtattt agcgataaaa ccattgagtt
    11821 cacgattggt tgattttaaa agatcatcaa cctgaggaat ctgtaatttt ttttgctctg
    11881 ccaagatatg attaaccgtt gcattgaccc cttcaacagc agactgacca aaatcaagta
    11941 gcgcattttg gtctgccaaa aaggtatcta ccaatgccgg ggctttggca gtaatagctg
    12001 tctgctggtc agcacttagt ttctcaaaaa aagagatttg gccatttgtg tcagttggta
    12061 agtctgagat aatatcggtg gttttatcgg ttttgacaac agcattgtca gcaatttggt
    12121 caatgtcaaa attaaaatct gccataaagg gactccttta gtctttttgg tgatgcgttg
    12181 ttcgtctttg agtagcacct tgcatgattc gaaggctaat atcaaaatct ttaagatcac
    12241 tttcattgag tcgacgaagg gtttcgtcaa gatcttcatc aaattgttgg atagcttgtt
    12301 tggcttgttc taaacgagca gcagcgttat agtaattttt aggctcttct ttgattttta
    12361 agtagcctgt taagatatcc tcaaacctcc tcatgttagc ttcatgcagc gcaagcaatt
    12421 cttcttgatt gtcggctcct tggattttag caaggatagc ttgatgatct gtctgaatat
    12481 tatcataata cggtttgagc tcaggtgctt tgttaataag attaggagat ggggttttca
    12541 aaggctgagg gttaagagtt gtggcaatct gcagtgtttc gagttgaagg gtaatatcac
    12601 tttcaacgtc attggctttt ttggtgatac gacgataaat tttgggatct aactggtctt
    12661 ttaacgtaat agcttttgct ttgatgtcac taagttgtgg taataattgc tgagcaagta
    12721 cgttatattg gaagtcatca tggtctgcca gataagtgtc taacagccga actttacgat
    12781 cagctagctg aatttttgct tttagatctt ctaattgttt ggcaatcttg gcctttgcta
    12841 gccgcttttg atgagatcta acacgaaaaa tagcataacc tgtaccacca atggctatgg
    12901 caggcaaaat gtctagaagt gactcagtaa aagaagagcc acctataaag cttgaaatca
    12961 taaaaaaagc aatgattagc caaatccagt ttagtgattt attttttgtg ctcgtgcggt
    13021 catactttct aatcatgtga tatttcctcg atatgttttt actggctatc tcatagaggt
    13081 aacttactat tattttacct taaaaccact atttctgaaa gaaaagagct aatgttttaa
    13141 gagaatcgtt taaccctgct aaagcttagg agaaacgagg ttaaaagctt ttctcttata
    13201 atgttcgttt ttttaggatt gtttatcatg gttaaaaaaa gagtttctct ttttccttaa
    13261 taatgctaag aaatctagca gaaaaaatca ttttttgcta taataagttg taagatatat
    13321 gctagcaggt gagattcctg cctgttttct ttaaatatca agagctacat tgctgatctt
    13381 acagatctga ccttcttttt ggtttttaag cagtggttag cttgcctatg atttttattg
    13441 agaataagtg tttcagaaag ttaggacaat gatatgacat ctgtagttgt tgttggtacc
    13501 cagtggggtg acgaaggtaa aggtaaaatc actgatttct tgtcagcgga tgcagaagta
    13561 attgcacgct atcaaggtgg tgataatgct ggtcatacga tcgtgattga tggtaagaag
    13621 tttaaacttc acttgatccc atcaggaatt ttcttcccgc aaaaaatttc cgttattggt
    13681 aatggcgttg tggtcaaccc caaatctttg gtcaaagagt tggcgtacct gcatgatgaa
    13741 ggagtgacga cagataacct tcgcatttca gaccgagctc atgtcatttt accttatcat
    13801 atccagctgg atcaactgca agaagatgca aaaggtgaca ataaaatcgg aacaactatt
    13861 aaagggattg gtccagctta tatggataaa gctgctcgag ttggtatccg tatcgcagat
    13921 ttactggata aggatatctt tgcagagcgc ctacgcatta atttagctga gaaaaaccgt
    13981 ttatttgaaa aaatgtatga cagtacacct cttgattttg atgctatctt tgaggagtac
    14041 tatgcttacg gtcaagaaat caagcaatat gtgacagata cttctgtaat tttaaatgat
    14101 gcacttgatg ccggtaaacg tgttcttttt gaaggtgcac aaggggttat gcttgatatt
    14161 gaccaaggaa cttacccatt tgtaacgtct tcaaacccag ttgctggtgg tgtaaccatt
    14221 ggttctggtg ttggcccaag taaaatcaac aaagtagttg gtgtctgtaa agcctacaca
    14281 agccgtgtcg gtgatgggcc attccctaca gaactctttg atgaagtggg tgagcgcatt
    14341 cgtgaagtgg gtcatgagta cgggacaacg accggtcgtc cacgtcgcgt gggttggttt
    14401 gattcggttg tcatgcgcca cagtcgtcgt gtatcaggta ttactaacct ctctctgaat
    14461 tcaattgatg ttctttcagg gcttgatacg gttaagattt gtgtggctta tgaccttgat
    14521 gggaaacgta ttgactatta cccagcaagt cttgaacaac tcaaacgttg caaaccaatc
    14581 tatgaagaat taccaggctg gcaagaggac atcacgggtg ttcgtagcct tgatgagctg
    14641 cctgaaaatg cccgcaacta tgtccgtcgt gttggagaat tggttggcgt tcgcatttca
    14701 accttctcag ttggtccagg acgtgaacag accaatattc ttgagtcggt ctgggcatct
    14761 atctaaggtg tctagtatga caaagagttg gatagcgttt ccagctcttt ttaccgcttg
    14821 ttggattgta tagttagtca tgctattact agtagcttga gttttgtcgt tgacttatct
    14881 ccttaagagg tgatttttga ggagataatg ggatttacca gttgatctca ctttatcgtt
    14941 cggtcatttt ggtgaaaaag agatttttca acaaaaatag taggataaaa ctgatttttt
    15001 tgatatacta aacttttcgt tctagagcat aagtagaacg ttaacaatga cacatcactt
    15061 taggattaaa catttaggag gttcattttc acatgaacaa aaaagtaatg tcacttggtc
    15121 ttgtttcgac tgccctattc acattaggag gctgtaccaa taactccgct aaacaaataa
    15181 ctgacaattc attaaaaatc gctatgatta ctaatcagac gggtattgat gacaagtcat
    15241 ttaaccagtc agcctgggaa ggcttgcaag cttggggaaa agaaaataaa cttgaaaaag
    15301 gaaaaggcta tgattatttc caatcagcca atgaatcaga gtttaccaca aaccttgagt
    15361 cagcagtaac caatggttat aatcttgttt ttgggattgg atttccatta catgacgctg
    15421 tagaaaaagt agccgcaaac aatcctgaca accattttgc aattgtggat gatgtgatta
    15481 aaggtcaaaa aaatgttgca agtatcacct tttcagacca tgaagcggca tacctagccg
    15541 gtgttgcagc agctaaaacg acaaaaacca agcaagttgg ttttgtaggt ggtatggaag
    15601 gagatgttgt caagcgcttt gaaaaaggtt ttgaagctgg tgtgaaatca gtagatgata
    15661 ccatcaaagt aagagttgct tatgcaggct cttttgcaga tgctgccaaa ggcaagacga
    15721 ttgcagctgc tcaatacgct gaaggcgcag atgttattta tcatgcagca ggaggcacag
    15781 gggcgggtgt ctttagcgaa gctaagtcta tcaacgaaaa acgtaaagaa gaagataagg
    15841 tttgggttat tggtgttgac cgtgaccaaa gtgaagatgg aaaatacacc acaaaagatg
    15901 gcaagtcagc taattttgtt ttgacctcaa gtatcaagga agtcggaaaa gctttagtaa
    15961 aagtagccgt aaaaacctca gaaggccaat tcccaggtgg tcaaataacc acttttggtt
    16021 taaaagaagg tggtgttagt cttacaacgg atgctctgac acaagacact aaaaaagcta
    16081 ttgaggctgc taaaaaagcg attatcgaag gaaccatcac agttcccgaa aactaaaact
    16141 gtacaatacc gtccagagtc gtcactaacc gactttggac tttttaataa aggtgtcaag
    16201 tagctccaca gatacgttga cagcagccct gtcttttatt ataatgaaga tgatagaaaa
    16261 cagcgcaaaa gccaaaaaaa gggctttttt ataatgaaag gaaacatcat gctagattca
    16321 tttgataagg gctggttcgt cctccaaact tactcaggct acgaaaacaa agtaaaagaa
    16381 aacctcttgc aacgtgccca aacctataac atgttggaca atattttgcg cgtcgaaatt
    16441 ccgacacaaa cggttaacgt tgaaaaaaat gggcaaacca aagaaatcga agaaaatcgc
    16501 tttccagggt atgtcttggt tgaaatggtc atgacagatg aagcttggtt tgttgtgcgt
    16561 aacacaccaa acgttacagg ttttgtggga tcacacggga accgatcaaa accaacacca
    16621 cttcttgaag aagaaatccg tgctatcttg ttgtctatgg gtcaaaccat tgatgtcttt
    16681 gataccaata tcaaagaagg cgatgtggtt cagatcatcg acggtgcctt tatgggacaa
    16741 gaaggtcgtg tggtagaaat cgaaaacaac aaagtgaagc tgatgctcaa catgtttggc
    16801 tctgaaacag tagcagaagt tgaactttat caaattgcag aactataggc tctcaaaggt
    16861 ctgatggtat aaatattacc atcagacctt ttttagttgg aagtgaagcg tgtgtacaaa
    16921 caaaggtatg actatcgtta acatcattgt aatcagagat aattaacaga tctattactg
    16981 ataacggtgc tactatcaaa tatttaacta gtacttttaa tctggtttaa atagaacaga
    17041 tgtgaaggtt ctgtgataat aagatggaaa tatccccttg aaacttgtaa cagttggcta
    17101 cttgtttttt tgtgttcatt tttttgatag aaaagtctaa aacgatgtta ataaatcgca
    17161 ttgacaaaaa ctatttgcta atgtatagtt tacttaaaaa taatataagg tggtttacat
    17221 gagaaacaaa aaagtaacat tagctcatat tgtcgcaaag acaagtgttg ctattgcttt
    17281 ggctggagca atgggtagca gtttattagc taatagcaca acgtacgctg ttagtggcaa
    17341 agaaaataaa aaaagcgatg tcaaatatga aacgaccaaa gttatggaag ctaacgcaac
    17401 ttcctctaaa gaagacaatc atgtcatgca cacattagac ggctcaatga gtactgtctg
    17461 ggaggaaaat tcacctggtg gtggtgttgg tgaggtactt tcctacaagt ttgcttcccc
    17521 gatgcgtatt gggagaattt taattgttaa tggagataca tctagcaagg agaattacta
    17581 caagaaaaat agaattgcaa aggctgatgt taaatactat aacgggaata aattggtcct
    17641 ctttcaaaaa attgaattag gcgacaccta cactaaaaaa ccgcatcaca ttgagattga
    17701 taaaaaatta gatgttgatc gtattgatat tgaggtaaca gaggtccatc agggacaaaa
    17761 caaggatatt ttagccttgt cagaggtcac ttttggcaat atggaacgcg atctttttga
    17821 aaaaaagttt aaagaaatta aagataaatg ggtaacagat aaacaagcag atgagtttat
    17881 tgaaactgcc gacaaatatg ctgataaagc tgttcagatg tctgctgttg cgtcacgtgc
    17941 tgagtattac cggatgtacg ttagccgcaa ataccactac aaaaaagagt ttgttgaaaa
    18001 actaaaacaa gtctacaaag aaagcggagc ttctcacgtt acaagtaaaa aagatttgat
    18061 gttagctttt gatgatgcta aaagaaagtc aacgattggt cgacaagaaa acggtctttt
    18121 tgtgacaagt tttgctgaag atatggcatt gctctttact gatcaaggta agttaaaatc
    18181 agctgaccaa attgaaaata taaaaggtgt cgatagcgga agatatagcg atggggttta
    18241 tcagtatgag tacgattctg aactaacaaa aaacattgat aagctaggct atattcgaac
    18301 agctagcgga gatactcctg gagcaaattc gctcaacatt cctggttgcc aaacgtggtc
    18361 aggaaaacac attgaaaatt cagaaagtga attaattttc ccatcgatta gtgttaaaga
    18421 tctaaaatct aaagctgtct tagcagagat tgatgccaaa ggctattttg aaattattga
    18481 tcctaccatc attgctccaa atggtgacca taaaaaagta actggtcgct tcaaaattaa
    18541 gaaaatgcaa gatagaatgc aagataggaa gtaacaatat gtataaggtg ccaaagggtt
    18601 tagaacatta ccaaaaaatg tttcaaaaag aagtaactgt taatgattta aaaaaatact
    18661 tgataggtag tgacaaagag tatcgtatca ctagaagaga ctcttatatg ggagatattt
    18721 ctgatccaga agttatttta gagaatgggg tttaccctgc tttcctaaaa ggctataccc
    18781 aattgaaagc taacatcgaa gaagcattat tagaaatgtc aaatagcggt caagcattag
    18841 acatttacca agcggttcaa accctaaacg ctgaaaacat gttattaaat tattacgaaa
    18901 gcttgccatt ttatttaaac cgtcaaagca tactagctaa tataaccaaa gcgttaaaag
    18961 atgcgcatat tagagaggct atggcacatt acaaattagg agaatttgct cactatcaag
    19021 atactatgct tgatatggtc gaaagaacaa tagaaacatt ttagaatgat aaaaaggtat
    19081 gaaggacatg tctaataaaa aaacatttaa aaaatacagt cgcgtcgctg ggctactaac
    19141 ggcagctctt atcattggta atcttgttac tgctaatgct gaatcgaaca aacaaaacac
    19201 tgctagtaca gaaaccacaa cgacaaatga gcaaccaaaa ccagaaagta gtgagctaac
    19261 tatcgaaaaa gcaggtcaga aaatggatga tatgcttaac tctaacgata tgattaagct
    19321 tgctcccaaa gaaatgccac tagaatctgc agaaaaagaa gaaaaaaagt cagaagacaa
    19381 aaaaaagagc gaagaagatc acactgaaga aatcaatgac aagatttatt cactaaatta
    19441 taatgagctt gaagtacttg ctaaaaatgg tgaaaccatt gaaaattttg ttcctaaaga
    19501 aggcgttaag aaagctgata aatttattgt cattgaaaga aagaaaaaaa atatcaacac
    19561 tacaccagtc gatatttcca ttattgactc tgtcactgat atgacctatc cagcagccct
    19621 tcagctggct gataaaggtt ttaccgaaaa caaaccagac gcggtggtca ccaagcgaaa
    19681 cccacaaaaa atccatattg atttaccagg tatgggagac aaagcaacgg ttgaggtcaa
    19741 tgaccctacc tatgccaatg tttcaacagc tattgataat cttgttaacc aatggcatga
    19801 taattattct ggtggtaata cgcttcctgc cagaacacaa tatactgaat caatggtata
    19861 ttctaagtca cagattgaag cagctctaaa tgttaatagc aaaatcttag atggtacttt
    19921 aggcattgat ttcaagtcga tttcaaaagg tgaaaagaag gtgatgattg cagcatacaa
    19981 gcaaatcttt tacaccgtat cagcaaacct tcctaataat cctgcggatg tgtttgataa
    20041 atcagtgacc tttaaagagt tgcaacgaaa aggtgtcagc aatgaagctc cgccactctt
    20101 tgtgagtaac gtagcctatg gtcgaactgt ttttgtcaaa ctagaaacaa gttctaaaag
    20161 taatgatgtt gaagcggcct ttagtgcagc tctaaaagga acagatgtta aaacgaatgg
    20221 aaaatactct gatatcttag aaaatagttc atttacagct gtcgttttag gaggagatgc
    20281 tgcagagcac aataaggtgg tcacaaaaga ctttgatgtt attagaaacg ttatcaaaga
    20341 caatgctacc ttcagtagaa aaaacccagc ttatcctatt tcatacacca gtgttttcct
    20401 taaaaataat aaaattgcgg gtgtcaataa cagaactgaa tacgttgaaa caacatctac
    20461 cgagtacact agtggaaaaa ttaacctgtc tcatcgaggt gcgtatgttg ctcaatatga
    20521 aatcctttgg gatgaaatca attatgatga caaaggaaaa gaagtgatta caaaacgacg
    20581 ttgggacaac aactggtata gtaagacatc accatttagc acagttatcc cactaggagc
    20641 taattcacga aatatccgta tcatggccag agagtgcacc ggcttagctt gggaatggtg
    20701 gcgaaaagtg atcgacgaaa gagatgtgaa actgtctaaa gaaatcaatg tcaacatctc
    20761 aggatcaacc ctgagcccat atggttcgat tacttataag taggactggt tcaagaggtt
    20821 cgtcaagcac cttgatgctg gttatctctt gagaccttgt gcctactcct ctcacaaata
    20881 cagaagaaag cttgtctatg agataccttc atagacaagc ttttttgata tactaatcaa
    20941 aaagttattc ttttctcaca tagtgacctt gaagctaagc tgcttctaat ctataatact
    21001 aacaagatac tttttaacaa gcaaagcata atataaaagg gagagataac tattatcatg
    21061 aaacaacaat cttaccagcc tctacgcctc gtctacctct tggtggcttt atttgctgct
    21121 atgttgctta tagcaagacc tgttatggcg gatggggaac aaaaagatcc gtgggaacta
    21181 ggctataacc aaggtttaac aaaggggaaa gaagatggtg aatcggcaaa gtggactgat
    21241 ttgaccccaa ctgtcccaac tgatccaact attccacaac cacctactgt tattgaagag
    21301 actaataaag gtatttataa agaaggatat aaagatgggt acaaagaggg ttataataaa
    21361 ggatttggag gaagctaccc tttgttaatt cctgtccagt ttatatgggg actcatttta
    21421 cattggtggg ggaagttgag ctgacatttg ataatcaact ctaagctgat tccatagcta
    21481 gtggctatag ttaggaaaaa acgaatttaa aacagttcct tgctaccaca ccttaaaccg
    21541 cactttaatc attaggtttt agtgatatgt gcccaggatt ctggggacat agcctatgtc
    21601 taattgttgg ggtgcggttt aattgtcttt gggtttgaag ttgtggcaac accctaactt
    21661 taggacagca aaaaagccca ggactgccta aataggcacc taagcttttg agtacttcag
    21721 acttactaat gagatatttc tcttaattta gatgtaatat tagtaaattt ttccgtctcg
    21781 gatacaatag taaagccaag tgatagggcc gcaataccct ctgaaattgt aaccacagca
    21841 atttgttcca tctgcacatg cttgagtagt tgtagtctca tttgctgaaa cagtttggtt
    21901 ttctggtgtt gcaaggctaa ggcctacaac agcagcagtt gcgatcataa ataaagataa
    21961 tttctttttc atttgtaact ccttaaat
//
LOCUS       DATUYR010000026        21565 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.026, whole genome shotgun sequence.
ACCESSION   DATUYR010000026 DATUYR010000000
VERSION     DATUYR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21565
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.026"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(158..457)
                     /locus_tag="VCI33_000947"
     CDS             complement(158..457)
                     /locus_tag="VCI33_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831880.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     INFDDLRLTMVDMVSLSSEYDVIVTDMILEQTMDSEILFFNQMAPSVVANRLTDM"
     gene            complement(614..1081)
                     /gene="perR"
                     /locus_tag="VCI33_000948"
     CDS             complement(614..1081)
                     /gene="perR"
                     /locus_tag="VCI33_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEP2831881.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQSDF"
     gene            complement(1133..1588)
                     /locus_tag="VCI33_000949"
     CDS             complement(1133..1588)
                     /locus_tag="VCI33_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEP2831882.1"
                     /translation="MIYSFQNPSEDILKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEVQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNL"
     gene            complement(1775..4417)
                     /gene="polA"
                     /locus_tag="VCI33_000950"
     CDS             complement(1775..4417)
                     /gene="polA"
                     /locus_tag="VCI33_000950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEP2831883.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     CEAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(4548..6275)
                     /locus_tag="VCI33_000951"
     CDS             complement(4548..6275)
                     /locus_tag="VCI33_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEP2831884.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGVFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADVGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAQKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     QSKVSSWTK"
     gene            complement(6291..7487)
                     /locus_tag="VCI33_000952"
     CDS             complement(6291..7487)
                     /locus_tag="VCI33_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEP2831885.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(7974..9065)
                     /gene="ulaG"
                     /locus_tag="VCI33_000953"
     CDS             complement(7974..9065)
                     /gene="ulaG"
                     /locus_tag="VCI33_000953"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEP2831886.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(9318..10964)
                     /locus_tag="VCI33_000954"
     CDS             complement(9318..10964)
                     /locus_tag="VCI33_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEP2831887.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INKALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQVNDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(11289..11993)
                     /locus_tag="VCI33_000955"
     CDS             complement(11289..11993)
                     /locus_tag="VCI33_000955"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEP2831888.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGSVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(11995..12858)
                     /locus_tag="VCI33_000956"
     CDS             complement(11995..12858)
                     /locus_tag="VCI33_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEP2831889.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLT"
     gene            complement(12863..13525)
                     /locus_tag="VCI33_000957"
     CDS             complement(12863..13525)
                     /locus_tag="VCI33_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEP2831890.1"
                     /translation="MTHLPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     IKEERGGRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            complement(13634..14119)
                     /locus_tag="VCI33_000958"
     CDS             complement(13634..14119)
                     /locus_tag="VCI33_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2831891.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNHNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKNSPYLEGLDLD
                     S"
     gene            complement(14242..14520)
                     /locus_tag="VCI33_000959"
     CDS             complement(14242..14520)
                     /locus_tag="VCI33_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2831892.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(14591..16024)
                     /locus_tag="VCI33_000960"
     CDS             complement(14591..16024)
                     /locus_tag="VCI33_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP2831893.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(16331..18832)
                     /locus_tag="VCI33_000961"
     CDS             complement(16331..18832)
                     /locus_tag="VCI33_000961"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEP2831894.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGNEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDKSKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(19044..20183)
                     /locus_tag="VCI33_000962"
     CDS             complement(19044..20183)
                     /locus_tag="VCI33_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEP2831895.1"
                     /translation="MKENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            20667..20957
                     /locus_tag="VCI33_000963"
     CDS             20667..20957
                     /locus_tag="VCI33_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831896.1"
                     /translation="MKNKLFLVALATVTVLGPSLATPHHQTVHATEQAKEPQTNSMTC
                     TNSGAVCAGWHSLTEEEDSITSNSSSKTNTSPLAWLWSLITSLWISATSLFT"
     gene            21132..21299
                     /locus_tag="VCI33_000964"
     CDS             21132..21299
                     /locus_tag="VCI33_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831897.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGSHQTVSANDASYSNGFYGHN
                     GPDSWLLYTVW"
BASE COUNT         6381 a         4754 c         4127 g         6303 t
ORIGIN      
        1 gctttttgtt ttttaatttt tcaagttgaa taattaaacc ttgcttttct ttttcaaggt
       61 aatagactct actccttgta agtctaactt attcttaatc ttatttatcg ctcatttttt
      121 aaattccgat gctctcacag atattatcat tgtttagtta catgtctgtc aatctattcg
      181 ccaccacact aggggccatc tgattgaaaa ataaaatttc ggagtccatt gtttgctcaa
      241 gaatcatatc tgtcacaatt acgtcatact ctgaagacag tgacaccata tcaaccatgg
      301 tcagcctcaa gtcatcaaag ttgataatct caaaaaatcc tccaaaatac tcttttaaga
      361 agttgccaac attgttataa cttctctcaa ccactaatag tttcaagcga cgacgcgatt
      421 gatttgtaag aaagagattt tcccaggtga ggaacaaaca aatagaggag atgattgatg
      481 gttctttcct ttaattcctt atgatcccca gcaaataact ctttaacata agcaacaaag
      541 gtctggtatt gaactggggg tttttgattt gtaaaggcac tatatttata atgcctttat
      601 ttaagggtta actctaaaaa tcagattggt cttttgcttg gcaatccgga caaatcccgt
      661 aagcaataac tgggatacgc gtgaccttat aacctgtttg ctcatgggct tctttggcaa
      721 tatccataac atcaacatcc ataaagtcag caatcttccc acatatttca caaaccacat
      781 tgacgtgttg atggcccata aagtcatagt aggtggttaa atcattgctg attttcaact
      841 ctgagacaaa accttcatca actaaaactt tcaaattatt atagactgtg gcaaggctca
      901 tgttaggaaa gttaggctgt aagtcacggt aaatcttatc ggcactaggg tgttctgtgg
      961 actgaatcat gtaagaaata atggctttac gtgtctctgt aattcgaata tgtttttccc
     1021 gtagatgctc taggacattt tcataggcat ctagggcttg ctgatgtgaa tgaatgtcca
     1081 taagctgcta ctcctatcct tttttccttt attatcacgc acgtctctag tttcaaagat
     1141 ttgtgatgca ttctggttta gcgactaatt gcaaatagtc tatttttagg cagcgattca
     1201 tcacaatagc ctctttgcct gccgaccgta aaatggtttg tacttcttgg ttttcaaggc
     1261 ctaattgcgc ccaaaagact ttggcctgac ctgctaaaaa gtctctggca acttctggta
     1321 aaaactcact tctgcgaaag acatctacaa tgtcaacctc aaagggaatt gccttgatac
     1381 tggcatagac cttctctccc aaaataagct gacctgctaa tttgggattg actggaatga
     1441 tccgataatc catagcctgc ataaatttgg cgacaccata agctgctgtg tctttgcggt
     1501 cagaaagacc aacaaccgca atggttttgg ctgactccaa gtaagctttt aggatatcct
     1561 ctgagggatt ttgaaacgaa taaatcactt gttcccctat ctatttaata acctctacag
     1621 ctgttcaata tagcaggata actcctaaag catggcttca atcactttta accttatccg
     1681 tcaaaaagct caaagcagct tcatgacttc tcctacaatt ttttagttta ttataacata
     1741 agttgtcaaa atgagaaata acaagtaaga aaacttattt ggcttcgtac cagctctgcc
     1801 cagtactttc atcgacacat aaaggaacag ctaaatcaac agcagcttcc atggtgtctt
     1861 tgacaagttt ttttatagca gtcaactcat catttggcac ttccaaaacg atttcatcat
     1921 gcacttgaag cagcatttta gctctaaaac cacctgcttg caaggcctta tccagattaa
     1981 tcatagcgat tttgagaata tcagctgcgc ttccttgaat gggcgagttg atggctgtac
     2041 gctccgcaaa agagcgaacg ttgaaattac gagagttgat atccggtaat tcacgacgac
     2101 gcttaaagag ggtctctaca tagcccttgt ctttagcctc acgcaccaca ttttccatat
     2161 aggctttaat cccaggatag cgttcaaaat aggtatcaat gtaagacttg gcctgtttac
     2221 gggtgatacc aaggttattt gacaaaccaa aatcagaaat cccatacaca attccaaagt
     2281 taacagcctt ggcattacgg cggtcattgg cagtaacatc tgctgccttg tcaatcccaa
     2341 acacacgcat agccgttgag gtatgaatgt ctgccccttc attaaaggca gcaatcaaat
     2401 gctcatcccc tgaaatatgt gccagtaccc gcaattcaat ctgtgagtaa tctgaactca
     2461 acaagacggc atcttcatga gatggggtaa aggccttacg aatcaaacgc ccctgttcca
     2521 aacgaatggg aatgttttga agattagggt ccacactaga caaacgccct gtctgggtga
     2581 gatcctgcac atagcgggta tgaatcttgc catcagccag aatataatcc tgtaaaccaa
     2641 tcacataggt ggattgcaat ttagtaatct ggcggtaatc caaaatttta gccacaattg
     2701 gagcaatcgg tgccaaacgt tccaaaacat caacagctgt agaataacct gttttggtct
     2761 tcttagtcat ctcaagaggt agttgcatct tctcaaacaa gatactgccc agctgtttag
     2821 gcgaattaat gttaaattct tggccagcca tatcataaat ctcttgggtg agcgcttcaa
     2881 taatgacctt gttttgctct gccatatctt gcaaagtcgc cctattaacc ttgatccctt
     2941 caatttccat tttagcaagg acattagcca aaggcaattc aatattctga tagaggtcta
     3001 gttgctcatg ggcagtaagc ttgtctagca tttgagactt gctatctaat aatactttga
     3061 ccttacgagc taagtgttct agtaaaacgt ctttatcagg aacagcccgt ttagcacctt
     3121 taccatagac tgtatcgtct tcttccaaag agatgtcagt aaaaatgcgg gcaatggtgc
     3181 taagctcatt gtcttctaca gttgacagaa ggtaattagc taatctagca tcatagctag
     3241 gtgctaccaa ctcaattcct aagtggctca acaagacctt gctacgttta aaatcataag
     3301 tggcaatggg tttttggaag acctgcttaa aactatccgt cgctaatagg ctaaggtcag
     3361 tggatgcgta aatctgctca ccatgtcccc aagcaaaacc aatgatggcc tcacaatggt
     3421 aattatcccg aagcgtttca aagtagaaga tgtcttctgc gctaaacaaa tcagcagaca
     3481 cattggtcac ttcttgatag gaaatggcag ctactggctc ttgaggcatc tggctagcaa
     3541 gacctttctt gagttgcaca aaatccatct catcgtaaaa ttgagagagg ctagcaacat
     3601 ctggaccatt gtagacaata tcatctaagc caattgtgat aggagatgct gtattgatag
     3661 tggcgagcgt tttagatagg aaagcttggt cacggtcatt gatcaagttt tctttcattt
     3721 ttgaggcctt gaatccatca atatgctcgt aaatgccttc caaactacca aattcatgaa
     3781 ggagcttgag cccagttttt tcaccgattt tggtcactcc tggaatatta tccgatttat
     3841 ctcccatcaa ggctttgagg tcaataaact ggtttggcgt caagcccatt ttttccatga
     3901 ggtaagccgg tgtgaattct tcaaattcgg caaccccttt tttagaaatt tcaaccacgg
     3961 tattttcgtc agttaactgg atcaaatctt tatcaccact gacaatggtc acatcaaaag
     4021 gcacttctgt gcgctccgcc attttatcaa gcgtcccgat aatatcgtct gcttcgtagt
     4081 gttccaactc ataataagca atcccaaggg ctgtcaacat ctcacgaata taaggaaact
     4141 gctcacgaaa ttcctctggt gtcttggcgc gacccgcctt ataatcggca tacatttctg
     4201 ttcggaaggt ggttttgccc gcatcaaaag ccaccagcac gtgagtaggc tggactcttt
     4261 tcatcatgtg gtctagcatc aaatgaaagc cataaatggc attggtatga agaccgctat
     4321 gatttttaaa gcggtcaatt tgattgtaga gggcaaaaaa ggcacgaaag gcaacagatg
     4381 agccatcaat taatagtaat ttattcttgt tttccatagc cttattataa cacagaggga
     4441 cgggcttttc tcctttaagc cattggtttt gctgataagt aaatagttaa cctaaaaagc
     4501 ctaggaaaat tcctagactt ctctatttgc tatttagaca aaagcactta ttttgtccaa
     4561 cttgatactt tagattggtt tgcctctacc cattttttgg cggcagcttc tgggctcatg
     4621 cctttgttga tgtcaagcat gactgcttcc atatcttttt gggtccagtg gaacttatca
     4681 atgattttgt agacatttgg aagatctttt ttcaagcctt tacgagcaat ggtattgatg
     4741 ttctcagtcg agccaaaggt ttctttagga tctttaaggt acttcaagtc atacttggca
     4801 aacatccagt gtggtgacca agctgtcacg acaataggat cttttttctt gatcgcttga
     4861 tccaaggaag tcgtcatggc tcctgttgat gccgccacta actcccaact agatagatta
     4921 tgatactctt ttagagtctt ttgggctgct gccatgatac ctgcacctgg ttcgatccct
     4981 gtgatttttt gatcggcttg tttgcttaag tcctcaatac tattaacatc tgtcatgtat
     5041 tttggaacag ctaaaccaag tttggtccct tttaggttag gtcctaaatc atctaatttt
     5101 gacttgtatt tctgatattg ttggccatgg gtgactggca accaagcact agttgaaaaa
     5161 tcagcgttcc catttgcaac ggtttgccac ataacggcat tgtcaagcgg agtcaaggtc
     5221 acatggtaac cttcgttttt caaaacttct gcaatcacat gagttgaggc aacctcagaa
     5281 tcccactgca cgtaagcgat gttaacagtt tcacccttat ctgccatacc agaagtcata
     5341 gccataatac cgcgtcctaa agcagctatc aaaaagacag caagggctgc tagaccaatc
     5401 catttattgg ttttgccagc tttggctttt tcttgaggtt tgctattgaa caactgtgtc
     5461 atacggtcaa gcacaatcgc caagataacc aaggcaagac cactgacaaa tccactacca
     5521 acatcagcgt gttgaagggc tgacaagact tcacgtccta agccaggagc accaatcatg
     5581 gagcctgtta ctaccatgga aagggctaac atcatggtct gattaacacc tgccataata
     5641 gtatttttag ctaaaggcaa ttccacttta aagagctttt gtttcccagt tgacccaaag
     5701 gcatctgagg cctcaatcaa ttctgttgga atgtcacgaa tggccaaatt tgtaaaacga
     5761 accgttggag gcaaggcaaa gatgactgag gcaaacactc caggcaccat accaatacca
     5821 aaaaaggcaa cggcaggaat caagtaaaca aaagccggca tggtttgcat aaagtccaaa
     5881 ataggattca caatctgacg gacagtggca tttttagcca tccaaatccc cagcggaata
     5941 ccaataagta ccgaaatcaa actagctact aacaccagag taaaggtatt cattagctgc
     6001 ttccataacc cttgattgta aataaagagc aatcctaaca aggtaaaggt tggcaaaggc
     6061 cattttttct tagcaaggaa aaacacccct gctgttacta gaacaataaa aagtaacggg
     6121 tggataaata aaagtgtttt ggtcatccaa tccatcaaaa agctaccaac tacttgcata
     6181 atatcaaata gacctgagaa agttttagtt agccactccg ttagctgctc caccagttga
     6241 gctactggta attttgtttg taatatcgtt tccaaaatgc atcactcctt ttagttaagg
     6301 tcttcgtctg agatattagc caaggcttca atcactcgtc ctcgaataat gacacctagc
     6361 agacgattat tatcatctgt cactgcaatc ggagcagatg aatcatagat aagaggcaaa
     6421 atatctgtaa taatagtatc ttttgagaca gttctcacat cgcgatcaat cacttctgat
     6481 agcggtaacc cttttttgcg cgcctctata gcggcatcgg ccgtcaaact accgactaat
     6541 tggcggcggc gattcgtcgc catcaacata gacacctctt cgttgtgcat acggttcaag
     6601 gcaacttgag gtccatctaa ttcaacagta gttgttaacg gtttgatcat gatattttgt
     6661 gctgtcaaga ctttagaacg gtccacatcc tcaacgaatt cacgcacaaa gtcattggct
     6721 gggttagtca agatttcctc accagtacca atttgcataa tttgtccgtc tttcatcaaa
     6781 gcaatccgat caccaatacg caaagcttca ttcaagtcat gactgataaa gatgatggtt
     6841 tgtttcatgc tgtcttgcaa atcaagtaat tcatcttgca tctcacgacg aatcaaagga
     6901 tcaagcgctg agaatgcctc gtccattaag agaattttag ggctattagc tagcgcacgg
     6961 gctaggccaa cacgctgctg catcccacca gatagttggt ttgggtactg gtctttaaaa
     7021 tctaataggc ctgaattatc aagggctttt tctgccaatc gctggcgttc ttctttggga
     7081 acgccacgta attccaaacc aaattcggta ttttccaaaa tggttttatg aggaaagagg
     7141 gcaaagcttt ggaaaaccat gttaatgtca tggcggcgca cctcacgcag ctgatctgct
     7201 gacatggttg agatgtcttt accttccagc aagatagatc ctgctgaagg ttcaatcaaa
     7261 cgatttagca tgcggacaag ggttgatttc ccacttcctg atagccccat aataacaaag
     7321 atttcacctt ttttgacctc aaaactagcg tcatagacac ctacagtagc gcctgttttc
     7381 ttaaaaatct cactcttatt tttgccagtc tttaccatct caagagctgc tttttgtttt
     7441 ttgccaaaaa ttttactgag atgtttgact tctaaaattg tttccattcg ttttttctcc
     7501 taggtaatcg tttatcatgt gaaatagtga catagctttg gcgaaatatt agccaatatt
     7561 taacaaagta gctctacaca attaataaac gtgtggtcac ttttttactt aaaaaaagaa
     7621 tctgccgaaa cagaacataa ctatattatc acgtgtggtc actttttgtc aaggacaata
     7681 actcgatctg taataccaca atgataggtg atcgctttca ttcttttagg tatggtcttt
     7741 taaccagtac aattttttat cacaactgat gactatttct ttgctgatta ggctattgtt
     7801 acattagaaa tccttctttt acctataata tctcttttgc ggccatcgta aataacgacc
     7861 acggtgactc ttaaacattt gctcatctct ccaaaaaaac tcacctaaga cagtgtccta
     7921 tgaagcgagt tagcttaatc aggcactatc atcttaggtg agtagggtgt tgcttatagt
     7981 aaagctttaa attggatatt agaatcttct aagaagcagt catcaaatcc acgaggatga
     8041 tggtattcga tacgattttg gtcttgtgga taggtgtact tgccaccaac ctcccagata
     8101 aagggatgga aatcgtattg gagacgttct ttgcgcattt tccacagttc aaggatttca
     8161 tctgttgatg ccataaagtt tgaccaaatg tcataatgaa ctgggataac gactttagca
     8221 cggagatttt ctgccatacg aagaaggtca acagaagtca tcttgtcttg aatcccaatt
     8281 gggttttccc catagttatt taacacaacg tcaatatcat aatcacgtcc atgtttagca
     8341 aagtagttcg aaaaatgaga atccgcacca tgatagatgg taccacctgg tgtttcaaaa
     8401 atatagttaa cagctttgcg agccatgtca tcatctgtga tagctaaacc tgctaaatca
     8461 ccatcttgag catcagctcc ttgtattggg agagttacca gacaggtgcg gtcaaacgac
     8521 tctacagcgg tgatcttaat atctttgaat tcaaagctat cacctggctt taagatcata
     8581 atacgatctt ttggcacacc ccaatttttc caaacttcac cacattcata aggtcctaca
     8641 aatttaacat gattaagttt tgggttattg ataatggctg ccgcagtatt gatatcgatg
     8701 tggtcactat gataatgaga aaccaaataa taatccaact cattaatcat aaatggatca
     8761 atcaccattg gttgggccct aagattgggc tgtaatttac gagctcctgc catattagcc
     8821 atttgatgtc cacgaaccat atccttaact tgcttggtcg ctttcccacg atttgaccat
     8881 aagtccatga caacattagc accgcctggt gtcttaatcc agataccaca gtttcctaac
     8941 caccacatcg caaagttgtc agctggcacg acttcttgct cgatttcttc atttaaccat
     9001 gttccccatt ctggaaacgt gttaaggatc catgattcac gtgtaatatc ttgtacttta
     9061 gtcattaggg tttcctccga atttgttctt cgtttctata ctttaagtat agactttctt
     9121 gtcaatgata aaaagaccaa gtgtcacaca gatctgtgtt caaaagtgga ctgacttttt
     9181 cattagctca gtctaatact gtctcatttt gcacactgta tctcgcatca atactgtatt
     9241 caaagctata ttaaaaacct cagttatatt tttctatgtc atagaaaact gaggtacttt
     9301 gacgacttaa cacttactca tagcagctcg cgatgaatga gtttttcaat tttagaaacc
     9361 aaggcataac gatctgattc atcttttaca acagcttcaa tagcttttgt gagctctgta
     9421 gaaaagcggg agtgctcact caagcgtcct tgctttgaaa agcgtatcaa tttgatgata
     9481 tcatcgtcac ttaaaatagg gttaacaata agcatcggga ttttagtctt tagcgtatct
     9541 gtagtagcaa caatcatatc gactgctaaa aggtcgtaaa cactctgata ctgttctgtc
     9601 gtaaaaacag cttcaatctg accatttgct agataccttt gacactgttt gaacaataac
     9661 ttttgaatgc ctatcccatc atctgaaaca ataacgagtt tggtcttttc ttgctctgta
     9721 ttattgtgac gcagctctcc cccaagatgg atagtcaagt aggcaatatc atcgtctgtc
     9781 aagctaatcg tccaatcttg ttccaaaata gtggcacaag attgagtcat agcaaacaat
     9841 tcttcgtatt tttctttaat atggtctgtt aagggattaa ctaaaaaaat cccataagct
     9901 ttacggtaaa cgagcgcctt acagtgggtc gtcaactgct tcagcaaatc ctgtttatgc
     9961 gtgaaatgta actgataacg gctctccaac tggtcaataa agtgactaat ggttgctcgc
    10021 atgtcgtcat aatcttgact ttccacgtga tgatcttgat cttttcgaaa agacaacatc
    10081 aacatcgcta ccatactaac ttcgatatca tccaaatgaa gcttaaaatt atggtaaagc
    10141 tctctagcta aatcctgagc aatgtgatat tctttacgtt tccaaatcaa atgaaattct
    10201 tgtttaagag cagactttgt ttctgaatca agacgcatat tacgataact taaaagcatg
    10261 aaaggtaaaa tctgcaacat gaattggcta tcttgggtat taattgtctt tcctaagcta
    10321 gcttgagata aaaagagata ctcatgaaaa taagtcaaaa tatctttaga aaaataaacc
    10381 gataagcctt gtatctctga gagtttacgg ttgaatatat caataaagct agtattgcca
    10441 ccttggtaaa cgtccactaa caatttgtac aggtattgaa taagagccat tggatgacaa
    10501 ccaaaataat atcccctagc ctttgtcgca tgaagctgaa tcttgtattg tttgtcttcc
    10561 agttcttccc ttaactcagt caggtcattt aaaatcgtgt tacgagacac atcattgacc
    10621 tgcatcagtt tatcaatggt cacgcgctca gtggaaaccg caatataaat cgaggatagg
    10681 cgtctgcgct catcactttt catgacataa ttataatcag tcacttcttc taataataag
    10741 cgacaagcag ctttttgatc cgccgttaaa agaatgccaa gtctaggata agagataatc
    10801 tgatctactc cctttggcaa agccttatta attttgtcaa gctggtaata aactttacga
    10861 cgcgactgat ttaaagcatg agaaatggcc ataactgtct caggtgtttc tagtttaagt
    10921 aaataagaca gcaagtcata actcttttta tctaaaatca tcatgtttac aaacctcctc
    10981 tctgaaaatc catccttgct tattttatca tgatttcagt gactgcgcag ccaagttttg
    11041 aaaaactcac cataataaga ttcgcttaat atgattcgag ctaatcacct caactgatcc
    11101 aataccaaca ccttatatct ggtctctaag aactccacaa catacggttc atccagtttg
    11161 tgctcattgg cgtttaaaca gttcatcgaa aaggacaagt caaaaggact tgcccaaaca
    11221 atgattaaca aaaccttgaa ctttttggct tctagtgaga acctgtgcta ataagccata
    11281 tcgtgacctc atttttgacc gtaataagca tttgggccat gtttacgcaa ataatgctta
    11341 tccataatgt atcttggcgc tggctctacg cgtggattga tttgttctgt caagcgattc
    11401 atcttagcca cttcttctaa gacaaccgag tggtaaacgg cttgctctgg ggttttcccc
    11461 cacgtaaatg ggccatgatt acgcaccacg attcctggaa ccgccatcgg gtcaagacca
    11521 cgcttgctaa actcttctaa aatcacgctg ccagtttctt gctcgtaagc cccatcaact
    11581 tctgcttttg tgagagaacg agcacaagga accgggccat aaaaatagtc agcatgggta
    11641 gtcccataaa acggaatatc acgacctgcc tgtgcccaac cgacagcttc tgtggagtga
    11701 gtgtggacaa taccgcccac ttctggccaa gctttataaa gttccacatg ggttggcaga
    11761 tctgaagaag ggttcaaatc accttcgacc acattcccat caaggtctgt caccaccata
    11821 ttttctggtg tcaagaggtc gtagtccacc ccagaaggct tgatgacaat acgtcctaat
    11881 tcacgacaaa cttcagagac attgccccac gtaaatttga caagaccgtg ttgtggtaga
    11941 gacttgttag ccgcacagac acgttcacgc atttcttgca agtttttcgc catattatgt
    12001 caaccccgct ttctcaataa gtgggtaaag gaagtcctga gcttctttga tagcagcttt
    12061 tgtttcctcc accgtgtcac aattttctga ccacatttca atcaaaaaag gcccattgta
    12121 gttggttttc ttgaggaccg caaacagctc ttgccagtcc acacaacctt gaccaaatgg
    12181 cacatcacga aactgccctt ttgaggtttc cgtgacagca taagtatctt tcaagtggag
    12241 ggctgcaatg gatttgtgcc cattgtaaaa ttcactccac aaatcattat gccaagcaga
    12301 aacattgcct gcatcaggat aaacaaagag ataaggcgag tcaatctctt tttcaactgc
    12361 caagtatttc tcaatagagt tgataaaagg gtcatccata atctcaatag aaagcatcac
    12421 ttgggcttct tcggcccagt cacaagattg tctcaagttt ttgataaaac gagcacgggt
    12481 ttcaggcgat ttttcttcat agtaaacgtc ataaccagct aattgaatgg tacgaacgcc
    12541 taagtcctgt gccaattcaa tacattgttt catcagttta agggacttgg cttcaatagc
    12601 aggatcattt gagcctagtg ggtaacgtct atgaccagaa aaacaaatgg tcgggatgcg
    12661 aatgcctgtt tcataaatgg ctttcactaa atcaagccgt tcttccttag tccactctaa
    12721 gcgcgctaag cgagcatctg attcatcaac agacatctcc acaaaatcaa aacctaagtc
    12781 ttttgcaaat tgcaagcgtt cacgccaggt aaactgtttt ggcgttgctt tttcataaat
    12841 accaatagga cgcgccataa agttaccccc agatacgttt gatttcatct ttgaaggcac
    12901 gcgccgctgc agcaggatct tctgcttcag taatgcctcg ccctgcgata aaggtaaaga
    12961 catcaacccc ttcaaaaagt ttcaaagtgt ctacatcaag cccaccagtt actgagacac
    13021 gaaagcccat gtcaatcaac gttttcacct tgttaaggtc tttttcaccc caagtttcgc
    13081 cagcaagaag ggcatcacga gattggtggt aaatcgcttg ggaaatccca gcgtcaagcc
    13141 ataattgagc ttgctcataa gtccaatcac cataaagttc gatttgaatt tccccacggc
    13201 caccgcgctc ttcttttatg gctttgaggg ctgcttccat ggttggaatc gtcgcacagc
    13261 aaatgcaggt catccagtca gcaccacgtt ttgcattgtt tttagcgacc gtaccgccag
    13321 cgtcagcaca tttggtatct gctacgataa ttttctctgg gaagaggctg cgtaatacct
    13381 caactagttc gcttcccact tgaagcaaac aaacggtacc agcttcaatc acatctactt
    13441 cgtgacccac agcaacggct gctttaacag ccccctgtaa gtcagaatgg tctaaagcaa
    13501 cttgtaaatt tggaagatgt gtcatgtgtt tttcctagaa ctctcagcgg ctatgaaaaa
    13561 gctgatgagg agttcttatc ctttcaataa tgattgtgag tcgttttatc cgtttaagtc
    13621 agtaagctat cgattaagag tctaaatcca aaccttctaa atacgggcta ttttttgatt
    13681 cttctaccat ttctagaacc tctttaggac tttggcaggc caccaagcgt tcaatggcgt
    13741 tatctagttc aaacaaggcc acaatttgtg ggatagccac tgttgtgtga atagaagggt
    13801 ctgtagcggc caacgtcaaa agaacagaaa cctctttacc atctgagaag gtaactggtt
    13861 ttgttagtgt aatcaaagca aatgcattgt gattaacgcc taaacctgct tctgcatggg
    13921 gcatagccat acctggcatc aacacataat aagggccgta tttttctgtt gatgcaatga
    13981 tggcgtcgta gtaagctgac gtcactgctt tgctatcaat caaaggttga acagctaatt
    14041 gaacagcctc ttgccaagta tcagcactta gccccaatcg gatggagttg ttgtcaataa
    14101 aggcttgttt taaattcata aaaactatct ctttctagtg tcttgctttg caacacacac
    14161 catttttact agttttatcc tactaaagag aaagagctga aaaccttcgt cacaactcct
    14221 tcgttttgat tcagaattag cttatagcac ttcttgtagt ttggttttaa tttcattatc
    14281 atccatcagg ttatcaagac caaccaagtg gcctttggta cgaccatcaa gctcatgaat
    14341 caagtggttt gatgccacta caatatcata gccagaagct agacctttag cttcaccaac
    14401 tgagcaagaa gctgactgaa tatctgtcac accaagttga cgaagcgcat tttcaacttt
    14461 cattttgatc accatagatg accccatacc attgccacat gctgttaaga ctttaaccat
    14521 tgttttttcc tccgaaaatg ttttattatt gttttgttaa aatagcagag ctatttctta
    14581 agatgcagtg ttattgagct tccccacggt agtaagcttc tttgtcctta gcttttgcga
    14641 attgcaattg tggaatggca agtaagaaga tgcaaacaag cacataccct gcaataccaa
    14701 ggaatttgaa gaggtaccca aatggtaacc atggaagaac caagtcgata ttaccgtgat
    14761 aaccacctgt aagtcctaaa agacctacag caacagcacc aagtgccacc tgaataatac
    14821 ctgagataaa ggaaagagct accgctgctt tccagccacc acgtttatct gcataaaccg
    14881 caattgccgc attatcaaag aatacgggaa cgaatcctgt gataattaag attggatttt
    14941 taaagataac aagaagagca atggtgatta attgaccaat caaaccaaag gcaaatcctg
    15001 aaagcaccgc atttgaagaa ccaaagccat aggaagctgc aacgtcaacc gctgggaatg
    15061 accctggaag caatttgctt gagatccctt gaaaggcatt ggtcaattca gaaacaaaca
    15121 tgcgaacacc ctgcatcaag ataaagagat aaactgagaa ggtgagagag gtttgcaaaa
    15181 tatacatgaa gaaagcttgc ttagctgggt tgaatgctcc aggtccgatt aagtccacat
    15241 cagacataat gtctggacca aggacagcca agatagctcc gaaaaagacc aacatcaaag
    15301 ttgctgacgc aacaacagta tcatggaaaa tgtttaagaa ggttggtaat tttaggttat
    15361 caaggttttc ttctttttta ccaaagaacg gtgcaacctt atccacaaac caaatcgcaa
    15421 attgttgttg gtgaccaatc gcaaacccac cgccacctgt aaggcgttga gttgcttcaa
    15481 ccgtcatatt tgagctgact gcccagtaaa gaccacagat gataccaact gcccacgcac
    15541 caaaggcatt ttggaattga gggattaaaa gaaggacaaa gaccgaaatc gtcgctgcct
    15601 gttggaccat gatgtgacct gtgatgaaaa gggtacgaac tttagtgacc ttgcgaagtg
    15661 caaccaaaag aatgttgacc ccaaatccaa tcaagagagc tgtcgtagca acactgatga
    15721 agcccatctc ttctaactta gtattagcag cagccaaacc aaaataagga tcaataacgg
    15781 cggctttcaa ttcaaatttc ttggcaagag caaccaagat cggacgaaaa gtcgttacaa
    15841 gaccgcctgc tccaacgttc aaaatcaagt aaccaacggt agcctttaca aaaccagcaa
    15901 agacctcata aatgggtttt ttaaggagta aatacccgat taaaaccaaa agacctacaa
    15961 agaacgctgg gttttgcaaa atattttgcg agaaccagtt taggggagct aggagcattt
    16021 ccataagaac tacctctttt caaagttaat aaagataaag tgtttatatg ttcattatag
    16081 gtaatgaaag cgttttaaaa aagaccaact atcacacaaa gaagtgttca atattagact
    16141 taagttaact tattttaaaa tttaactaca cttaataata aagagaaaat caccctaaac
    16201 aacgattaaa aaccacaaga acgaaggttc ttgtggtttt tctagatgag ttgctttttg
    16261 aatagatcag ctctttggca ttaactgtga cgtgacgcaa ataaccactg aagggctgtg
    16321 tcacctcaaa ctatttgata acaatattaa cgagtttgtt tggtactgcg atgactttga
    16381 tgatgtcttt gcctgcgatt tcggcttgga ctttttcgtt agcaagagca acttcttgca
    16441 attcttcgcg tgaaaggtct ttggcaacga caagtttagc tttgacctta cctttgattt
    16501 ggacaacgat ttccacatcg ttttcaacca atttgctttt atcataactt ggccatggca
    16561 cgtaagagat tgactcacct gaggctgtca agacttgcca gagttcctca cctaagtgcg
    16621 gtgcgaatgg tgcaatcaat tgaacgaagc ctttggcata gtcgctaaag agtttgtctt
    16681 ccttgttggc tgcattgaca aataccatga gttgagcgat agctgtgttg aatttcattt
    16741 ggtcaacttg ctcagtaaca gccttaacag tttcgttgta aaccttgtca agggctccgc
    16801 tattttcctc agtgatctct ttagttgtga tcaaacggta aacacggtcc aagaacttac
    16861 gtgagccttc aagaccttct tcagaccaag cgatggaagc gtcaagtggt cccatgaaca
    16921 tctcgtaaac acgaagagtg tcagcaccgt actgctcaac aacatcatct gggttgacaa
    16981 cgttcttaag tgatttagac atcttagctg gtgcttgttc aagctcttca ccagtttcca
    17041 catggaagaa gctgccatca cgtttttcaa ccttgtcagt cgctacaagc gctccacggc
    17101 tgtcgcggta gcttgttccc aaaatcatcc cttggttaaa gagtttttgg aatggctctt
    17161 tggttggtac gacaccgaga tcgtaaagaa ccttgtgcca gaagcgagcg tagagaaggt
    17221 gaagcacggc atgctctgca ccaccgacat agatatcaac tggcaaccat tgtttgagga
    17281 gttcctcatc agccagtttt tcggtgttgt gtggatcgat gtaacggaga tagtaccagc
    17341 ttgaacctgc ccattgtggc atggtgttag tctcacgacg acctttaaca ccatcttcac
    17401 gagtcacttc caaccagtct gtcacgttag caagtgggct ctcaccagta cctgaaggac
    17461 ggatatcctt cgttactgga agaaccagtg gcaactcact ctcaggaacc gctgttgagg
    17521 tcccatcttc ccagtggatg attgggattg gctcacccca gtaacgttga cggctaaaga
    17581 gccagtcacg aagacggtaa gtgactttct cattaccgac gccttctgct tcaagccatt
    17641 cgaccatttt ggcaatagct gacgctttgt ccagaccatc taggaagtct gagttgatgt
    17701 gaagaccgtc ttctgtaaag gcagcctctt caacattacc accttcaaga actggaatga
    17761 tatctagctt aaattgtttt gcgaattccc agtcacgttc atcgtgggct ggaacagcca
    17821 tgatagcacc agtaccgtag ctggcaagga catagtccgc aatccaaact ggcatttcgt
    17881 taccattaac tgggttaatc gcatagctac ccgtccagac accagttttt tctttggcaa
    17941 ggtctgtacg agcaaggtct gatttgagac ttgcttgacg tttgtagtca gccacggctt
    18001 ctgcctggtc agctgttgta atagcatcaa ccaaggcatg ctcaggagca agaaccgcgt
    18061 aagttgcacc aaaaagggtg tcaggacgtg ttgtaaagac agtgaagtcc ttatctgtat
    18121 ccttaacctt gaaggttaca ttagcaccag ttgatttacc aatccagttg cgttgcatgt
    18181 ccttgattga ttctggccag tcaacttctt ccaagtcttc tagcaagcgc tctgcatagg
    18241 cagtgatttt gagcatccat tggcgcatgg gttttctgac aactgggtag ccgccacgtt
    18301 ctgatgtccc atcagggagc acctcttcgt tggcgatagc tgttcccaat tcttcaaccc
    18361 agttaacggg aacttcagct tcgtaggcta ggcctttttc ataaagcttg gtaaagatcc
    18421 actgcgtcca cttgtagtag ttagggtcag ttgtgttaac ttcacgatcc cagtcgtatg
    18481 agaaaccaag agcattaatc tggcgtttga agttagcgat gttttcagct gtaaactcgg
    18541 ctgggtcatt accagtatcc atagcgtact gctcagcagg aagtccaaag gcatcccaac
    18601 ccattgggtg gaggacattg tgaccttggg cacgtttgaa acggctgaga atatccgttg
    18661 ctgtatagcc ttctgggtga ccaacgtgga gaccagctcc tgatgggtat gggaacatat
    18721 ctagggcata aaacttaggt tttgatgcgt ctgttcctgt tttaaaagta tgattatctg
    18781 cccaaaaggc ttgccatttg ggttcaatag cggtgtgatc gtaaaatgtc atgtgaaatc
    18841 tcccatgtaa aaaatctagt cttcttatta tagcatgaga tgaagctttt aggaatggtc
    18901 aagacatgac tagcaccttg gcttttgaga cttcagacga ttagctattt ttaaaccctg
    18961 ctttagcatc aaccatcctc acaaaaagct aataaaaaaa gccagacttg ctctgtaaac
    19021 agagccaatc tggacaaata gacttaaaaa gcaagtgctt gctcaaaatc ggccaataaa
    19081 tcttcgatat tttcaacacc acaagacaag cgcagcaaac catcaacaac cccttgttcc
    19141 aaccgcgctt gtgatgataa acaagcatga gacatcgttg ctggatgtga caaaatggat
    19201 tctactccac ctagactaac ggctaaaatc ggcaactgaa tacgatgggt aaaggtcatc
    19261 agctcctcct tagaagccaa ctcaaaggat aaaacagcac caccattctt agcttgttgc
    19321 aggtgggtat cgtgattagg atgactgtct aagccagggt aatgtacctt aagaactttg
    19381 gggtgcttcg ctaaatagtt agcgagctgc tgagcatttt ttaccgcttg ttccatacgg
    19441 atccccatag ttttcatgcc acgaagaatc agccaagcgt cttcgacccc aacaattgct
    19501 ccaaagtttt tttgaaaaag cttgagctgg ttgtagatag cctcattatt ggtagcagct
    19561 aaaccagcta ccacatcact atgaccattg ataaatttgg tcacactttc taccacaata
    19621 tccacaccca tagctaaggg ttcttgatac aaacttgtca taaaggtatt atcagccaca
    19681 gttagcacac cataagcttt ggcgagttgc acaagctctg aaatatcaca aattttcaag
    19741 agcggatttg acggtgtctc tagataaatc atacgcgtat taggcctgat acttgctttt
    19801 actgttgcta aatcagccat atctacaaag cttgtttcaa tctgataatt gggtaaaata
    19861 gctgtcgcaa attgacaagt ccctccatag acctccaatg ggaaaatcac atgatctcca
    19921 gcttttaaga gcatcaaaac agttgaaata gcagccatac cagatgcata agccaccgca
    19981 taagtcgctt tttctaaaca ggctaacccg tcttctaaag cttcagtcgt tgggtttgaa
    20041 aagcgtgtgt acaaatgttt ttgactagga caatacagtt ctgaattgtg gaacgtcgaa
    20101 gtctgataga tgggaactga agctgctcct gtaaactcgt caataaccgt atagccatgt
    20161 aataaagtag tgttttcttt catttcgtgc acctccgcaa tagcgttgac tccaatgaac
    20221 tctcttttag acaatggtcc caccccaatg gttgcacaag ccaaaaagct cttcaaaaaa
    20281 gcgtgatctg ctaacctata agaaaagtag tgggcacctt ttaaacggat aatgaatttt
    20341 tccaacaata tcatttttag atcgattacg tttattatac aacctttagc taagtttgca
    20401 agccctttta tcatctttga ccaagctgcc aacacctctc cagcaagtta aagtatagct
    20461 acctttggtc ccatcgtctc aaaagcttag cttatcacac tgacagtcct actttgcatc
    20521 gtcacctact caatacggca atattgccat caaaatttca ttaaaaggtc acaatttatt
    20581 tcatttaatg agaaacagaa cttgttttca ccatgcgtct tatagtacac tgctaaagta
    20641 ctatttattt ttaggagaga aatatagtga aaaacaaatt atttttagtt gccctcgcga
    20701 ccgtaaccgt cctagggccg tctttagcaa cccctcatca ccagaccgtg catgcgactg
    20761 agcaggcaaa agaacctcag accaactcaa tgacgtgtac taacagtgga gctgtatgcg
    20821 ctggctggca tagcttaaca gaagaagaag acagtataac ttcaaattca tcttcaaaaa
    20881 ccaatacttc cccgttagct tggttatgga gcctaataac aagtttgtgg atttctgcaa
    20941 caagcttgtt cacctaagat ctctataatc atatccatgg aatttaaata accatctcgt
    21001 ggcggttttc taatcctagg tcgccacatt tcttattcag ataaataata ataaaaacat
    21061 taaaataata cttgatttat gaacaaaaag ttagtataat ataatcacaa tacaaattaa
    21121 aggagttatc tatgaaaaag aaattatctt tatttatgat cgcaactgct gctgttgcag
    21181 gccttagcct tgcaacacta ggaagccacc aaactgtttc agcaaacgat gctagctata
    21241 gtaacggttt ctacggacac aatggtcctg acagctggtt actttacaca gtatggtaaa
    21301 cctcataccg taatatagaa gctatcttct tcgtaagact acggttatga aaagcctaag
    21361 gctgcataag cagccttagg ctttttgatt ggctaaaacg tttttgccaa tgacacctca
    21421 aggtaaaaca gaaaaggcac actgcctata aaaggagaga agcccaaaac acaagtcgta
    21481 acagttgcta gctaaccagc accaaaaaag ctgtaacacc tcaaatagaa tgataaccag
    21541 attaatatta ttagtaatta atgtg
//
LOCUS       DATUYR010000027        20986 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.027, whole genome shotgun sequence.
ACCESSION   DATUYR010000027 DATUYR010000000
VERSION     DATUYR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20986
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.027"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(406..792)
                     /gene="rplQ"
                     /locus_tag="VCI33_000965"
     CDS             complement(406..792)
                     /gene="rplQ"
                     /locus_tag="VCI33_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEP2831898.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(807..1745)
                     /locus_tag="VCI33_000966"
     CDS             complement(807..1745)
                     /locus_tag="VCI33_000966"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEP2831899.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1791..2174)
                     /gene="rpsK"
                     /locus_tag="VCI33_000967"
     CDS             complement(1791..2174)
                     /gene="rpsK"
                     /locus_tag="VCI33_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEP2831900.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2192..2557)
                     /gene="rpsM"
                     /locus_tag="VCI33_000968"
     CDS             complement(2192..2557)
                     /gene="rpsM"
                     /locus_tag="VCI33_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEP2831901.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2575..2691)
                     /gene="rpmJ"
                     /locus_tag="VCI33_000969"
     CDS             complement(2575..2691)
                     /gene="rpmJ"
                     /locus_tag="VCI33_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEP2831902.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2717..2935)
                     /gene="infA"
                     /locus_tag="VCI33_000970"
     CDS             complement(2717..2935)
                     /gene="infA"
                     /locus_tag="VCI33_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEP2831903.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3053..3691)
                     /locus_tag="VCI33_000971"
     CDS             complement(3053..3691)
                     /locus_tag="VCI33_000971"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEP2831904.1"
                     /translation="MNLLIIGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3841..5145)
                     /gene="secY"
                     /locus_tag="VCI33_000972"
     CDS             complement(3841..5145)
                     /gene="secY"
                     /locus_tag="VCI33_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEP2831905.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5162..5602)
                     /gene="rplO"
                     /locus_tag="VCI33_000973"
     CDS             complement(5162..5602)
                     /gene="rplO"
                     /locus_tag="VCI33_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEP2831906.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5817..5999)
                     /gene="rpmD"
                     /locus_tag="VCI33_000974"
     CDS             complement(5817..5999)
                     /gene="rpmD"
                     /locus_tag="VCI33_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEP2831907.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6014..6508)
                     /gene="rpsE"
                     /locus_tag="VCI33_000975"
     CDS             complement(6014..6508)
                     /gene="rpsE"
                     /locus_tag="VCI33_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEP2831908.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6527..6883)
                     /gene="rplR"
                     /locus_tag="VCI33_000976"
     CDS             complement(6527..6883)
                     /gene="rplR"
                     /locus_tag="VCI33_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEP2831909.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(6988..7524)
                     /gene="rplF"
                     /locus_tag="VCI33_000977"
     CDS             complement(6988..7524)
                     /gene="rplF"
                     /locus_tag="VCI33_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEP2831910.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7727..8125)
                     /gene="rpsH"
                     /locus_tag="VCI33_000978"
     CDS             complement(7727..8125)
                     /gene="rpsH"
                     /locus_tag="VCI33_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEP2831911.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8276..8461)
                     /locus_tag="VCI33_000979"
     CDS             complement(8276..8461)
                     /locus_tag="VCI33_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEP2831912.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8477..9019)
                     /gene="rplE"
                     /locus_tag="VCI33_000980"
     CDS             complement(8477..9019)
                     /gene="rplE"
                     /locus_tag="VCI33_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEP2831913.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9043..9348)
                     /gene="rplX"
                     /locus_tag="VCI33_000981"
     CDS             complement(9043..9348)
                     /gene="rplX"
                     /locus_tag="VCI33_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEP2831914.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9427..9795)
                     /gene="rplN"
                     /locus_tag="VCI33_000982"
     CDS             complement(9427..9795)
                     /gene="rplN"
                     /locus_tag="VCI33_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEP2831915.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9820..10080)
                     /gene="rpsQ"
                     /locus_tag="VCI33_000983"
     CDS             complement(9820..10080)
                     /gene="rpsQ"
                     /locus_tag="VCI33_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEP2831916.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10106..10312)
                     /locus_tag="VCI33_000984"
     CDS             complement(10106..10312)
                     /locus_tag="VCI33_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEP2831917.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10322..10735)
                     /gene="rplP"
                     /locus_tag="VCI33_000985"
     CDS             complement(10322..10735)
                     /gene="rplP"
                     /locus_tag="VCI33_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEP2831918.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10739..11392)
                     /gene="rpsC"
                     /locus_tag="VCI33_000986"
     CDS             complement(10739..11392)
                     /gene="rpsC"
                     /locus_tag="VCI33_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEP2831919.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11405..11749)
                     /gene="rplV"
                     /locus_tag="VCI33_000987"
     CDS             complement(11405..11749)
                     /gene="rplV"
                     /locus_tag="VCI33_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEP2831920.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11765..12043)
                     /gene="rpsS"
                     /locus_tag="VCI33_000988"
     CDS             complement(11765..12043)
                     /gene="rpsS"
                     /locus_tag="VCI33_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEP2831921.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12183..13016)
                     /gene="rplB"
                     /locus_tag="VCI33_000989"
     CDS             complement(12183..13016)
                     /gene="rplB"
                     /locus_tag="VCI33_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEP2831922.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13034..13330)
                     /locus_tag="VCI33_000990"
     CDS             complement(13034..13330)
                     /locus_tag="VCI33_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEP2831923.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13330..13953)
                     /gene="rplD"
                     /locus_tag="VCI33_000991"
     CDS             complement(13330..13953)
                     /gene="rplD"
                     /locus_tag="VCI33_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEP2831924.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(13977..14603)
                     /gene="rplC"
                     /locus_tag="VCI33_000992"
     CDS             complement(13977..14603)
                     /gene="rplC"
                     /locus_tag="VCI33_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEP2831925.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14851..15159)
                     /gene="rpsJ"
                     /locus_tag="VCI33_000993"
     CDS             complement(14851..15159)
                     /gene="rpsJ"
                     /locus_tag="VCI33_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEP2831926.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15358..16647)
                     /locus_tag="VCI33_000994"
     CDS             complement(15358..16647)
                     /locus_tag="VCI33_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEP2831927.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(17035..18051)
                     /gene="adhP"
                     /locus_tag="VCI33_000995"
     CDS             complement(17035..18051)
                     /gene="adhP"
                     /locus_tag="VCI33_000995"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEP2831928.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVAPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18303..20945)
                     /gene="adhE"
                     /locus_tag="VCI33_000996"
                     /gene_synonym="adhC"
     CDS             complement(18303..20945)
                     /gene="adhE"
                     /locus_tag="VCI33_000996"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEP2831929.1"
                     /translation="MTEGHNTVETTSVSVTINALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKIADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
BASE COUNT         6020 a         4472 c         3797 g         6697 t
ORIGIN      
        1 taacaatata gtttgagcat ctgtttgcga tgcaagtttt tttcggacat ttaaacgagg
       61 ggattacacc gtttatttgc ccatgagagg tctttaaaca cctgcataag gttttcttat
      121 tctgatgacc ttattgtctc tgagaaaact ttataaacgc atcagaagag gcttctgtgt
      181 tattgtttta ctcagcttaa aagcgggagt gtcacctcat aatacaacaa aacgcctgct
      241 tataaaaagt ttctatagct tagatacctt tcataaacaa gcgtcacata ttaacaatat
      301 gatgagtgtt cccgctagga aaaatcctag cggtagacca gagctagact aagaataatc
      361 tctcgattac tccatcatca taacactcaa caaaattgat aaaaattaaa ctaattcaat
      421 aattgccatt ggagcagcat ctccacggcg tggttctgtt ttaagaatac gagtgtatcc
      481 accgttacgt tctgcataac gaggtgcaat ttctgagaaa agtttttgaa gagctgtcgt
      541 tgatgtgtat ttatcggtag cttcatcata attttctgat gcgatttcgt tacgaacgta
      601 tgcagctgct tggcgacgag catgaagatc accacgttta ccaagagtaa tcattttttc
      661 aactgtttta cggatttctt ttgcacgtgc ttctgtcgtt acaattgatt cgttgatcaa
      721 aagatctgtc gtcaaatcac gaagcattgc tttacgttgt gagctagtgc gtcctagttt
      781 acggtaagcc attttgtcct cctatattat ttatcgtttt ttagtccgag acctaagtca
      841 gcaagtttaa ccttaacttc ttcaaggctc ttacgtccaa gattacggac tttcatcatt
      901 tcaggctcag atttttctgt taagtcgaag acagtgttaa tgcctgcacg ttttaaacag
      961 ttatatgagc gtacggataa atcaagctcc tcgatggtgc ggtcaagtac cttttcatcg
     1021 ttcacttttt cagtttcttt cattacttcg gtagcttttg caacctctgt taaatcagta
     1081 aacaggttta agtgttcgat caaaactcga gcagatagac ctaaagcatc ttcaggaata
     1141 attgttccgt ttgtcatgat ttcaatcgtt aatttatcaa agccatcatt gctacctaca
     1201 cgggcaggtt caacttgata attaactttt ttaactggcg tatagattga atctactgcc
     1261 aatgtaccca caggtgcatc atcttttttg tttccttctg ctggcacata accacgtttt
     1321 ttagcaacag tcattgttgc acgtagagaa tgtccttcag cgattgtaaa aagataatga
     1381 tccgggttaa caagttcgat atcactgtca gtaagaatat caccagctgt tacctctgct
     1441 ggtccttcta cttcaagttc gataatcttt tcgtcttcga cgtatgattt cacagcaagt
     1501 cccttaacat taaggataat ttgcatgaca tcttcacgta cacctgggat tgtatcaaat
     1561 tcgtgtagta ctccatcaat tttaattgat gttactgctg cacctggaag tgaagacaag
     1621 agtacacgac gaagagaatt acctagagtt gttccgtagc cacgttcaag tggttcgatg
     1681 acaaatctac cgtaatcttt attttcatca atttttgtta ttattggttt ttcaaactca
     1741 atcatttttt cacccctcga aacgaaactt gtgtactatt tgttatatga ttatacacga
     1801 cgacgttttg gaggacgagc accattatgt ggcacagggg tcacatcacg aattgcagtt
     1861 acttcaagac ctgcagctgc tagagcacga atagctgatt cacgacctga accaggacct
     1921 tttacagtca cttcaacagt ttttagtccg tgttcttgtg cagattttgc agcagcttct
     1981 gcagccattt gagcagcaaa tggagtagat ttacgagaac ctttgaaacc aagagcacca
     2041 gctgatgacc atgcaagagc attaccatgc acatctgtaa tcataacaat agtgttatta
     2101 aatgtagcgt gaatgtgtgc aacaccagat tcgatgttct ttttcacacg acgtttacgt
     2161 gttggtttag ccaattgttt tacctcctat tttatttttt cttacctgca atcgcaacag
     2221 ctttcccttt acgagtgcga gcattgtttt tagtattttg tccacggaca ggaagtccac
     2281 gacgatgacg gattccacgg tatgaaccga tttccatcaa acgtttaatg ttcatgttta
     2341 cttcacgtcg aaggtcacct tcaactttga ttgcatccac ttcacgacgg atagcatctt
     2401 cttgatctga tgttaaatct ttaacacgga tatcttcaga aatacctgca gctgctaaga
     2461 tttttttaga tgttgcaaga ccaattccgt aaacataagt aagtgaaatt actacgcgtt
     2521 tatcatttgg aatatcaact ccagcaatac gagccatttt ttctcctttc tattttatcc
     2581 ttgacgttgt ttgtgttttg gatttgtcgg acaaattacc ataacacgac cattacggcg
     2641 aataacttta cagtattcgc aaattggttt aaccgatggt cttaccttca tgttttaatc
     2701 cctccaatta tttcgattat ttaaagcggt atgtgatgcg tccacgtgtc aagtcataag
     2761 gactcatttc tactgtaaca cggtcaccta ctaaaatacg aatgtaattt ttgcggattt
     2821 ttcctgatac agttgctaga atttgatgtc cattttccaa ttcaacagta aacattgcat
     2881 ttggcatcgt ttctactact ttgccttcaa tttcaatcac gtcttctttt gccacgcaaa
     2941 agtaccccct aaatttcgat ttgatgtcct ctgagcacag aggtaacaat tataagtcag
     3001 actaaactag tatatcatta ctaatctact tatgcaagta gattagtata atttatttga
     3061 gttctagcaa cgctttttca acatctgcaa aaacgtctgt tatttcttga ttaccttcaa
     3121 tatctgtaac aagaccaagc ttacgatagt gttcaagaat aggttctcct tgagccatat
     3181 tgacgtccaa acgacgttta acagtttcag gcttatcatc ttcacgttga tagtaatctt
     3241 cttctttata atctactggt gggttgaaca ctttatggaa cgtttcacca gttttacgat
     3301 tgataatacg accactcaaa cgctctacaa gacatgatgg atccacttta atattaataa
     3361 caccatctaa gcggagtcct agttcttcaa gtgttgcatc taaggcgtgt gcttgttcaa
     3421 tagtacgtgg atacccatca agcaaaaaac ctttttctgc gatatcatcc tcagctaagc
     3481 gctcttttac aatcccgttt gtgacttcat caggaaccaa ttcaccttta tcaatataac
     3541 ttttagctaa acgtcccatt tcggtttgat tagccattgc ggcgcggaac atatcccctg
     3601 ttgagatgtg agcaacacca aattcttcaa caatcttagc tgcttgggtc cctttaccag
     3661 ctcccggcaa acctatgatt aaaagattca tcttttcctc ctcttttaaa ataaatacat
     3721 agtaggaaat tattttcctt ggtttttgtt ttcaaatggc aacaagaaat cgtttgatga
     3781 taaccaagtc ttattgtcat ttaaaaacaa aatagaaggt tgacaatgtc aacctgtatt
     3841 ctattctgct gtattcataa atccgacata ttttctcttt agaagatacc cctcaagctg
     3901 tttcatacct tcgataccag ttgaaatcaa aatgagcaaa ctagttccac ctaaagcaat
     3961 actagaagaa aggttgagag cttgttgtgc tgctattggc gctaaagaga taaatgctaa
     4021 gaagatagct cctacggttg ctagtttttt tagcaatgcg gacataaatt gttctgtctc
     4081 acgtccaggg cgaacacttg gtatatatga ggaattcttc tgaagatttt ctgctgtctt
     4141 ctcaggatta acttgtacaa aggtatagaa gaatgagaac aatataatca acaaggcgta
     4201 aactatcatt ccgactggag tttgataatt aaaaatttct tgcaaacggt ttaaccacgg
     4261 taaatctcta ccattttgga caaaaggaat aatcgtactt gggatagttg taatcgagct
     4321 agcaaagata acgggaataa cgccggctgg atttactttt aatggaagat atgaacttgt
     4381 aggtgcacct tgcattagct ttgtgtattg gattggaatt ttatattccg cttgttggac
     4441 atatgttgta aagaaaacaa tagcaagaac agctatgatt aaaatcccaa caatgagata
     4501 agacgagtgt aaatcacttg ccttaacatt tacaaaatag tcttcacgaa ttgttgcaat
     4561 tgcacttgga atagacgaaa taatacccgc aaagataatc attgatacgc cattaccaaa
     4621 tcctttatcg gtgatttgtt ctccaagcca ggtaacaata acgctacctg ttgtcagtaa
     4681 tgccccgatc agtaaatatg ttttgatgtc tggtgtctta acaagtgcaa cgttcgacaa
     4741 agtattaaac cctgcagtga taccaatgga ttgggcaaaa gccaaaacaa gtgaaatata
     4801 gcgcgtcgct tgatttaatt tacgacgtcc aacctcacct tgtttgcccc actcaacaaa
     4861 cttaggtaaa atatccatct gcaataactg aacaacgata gaagcagtaa tatatgggct
     4921 aacccccatt gaaaatactg agaaatttct catcgcatta ccactaacta agtttaacat
     4981 atttaagaaa gggagttcac ttaattgctc taagctcttg gcatttacac caggtacggt
     5041 aatatgtgtc ccaattcgga atacaagaat gataaatata gtaaagaaaa ttttattccg
     5101 tacagtcttt atctttaacg cgtcttttag tatttttaag aacataatga gtatcccctc
     5161 attagatgac ttcgattgaa ccacctttag caataatagc tgcttcagca gattttgaga
     5221 acttagctgc ttttacagtc aattttttag tcaattcacc gttaccaagc actttaacgc
     5281 ctgatttttc agctcggaca ataccagcat cttttaaaat agccggagtc acttctgtac
     5341 catcatcaaa aacattcagt tgatcaagat tcacaagtgc atattcttta gtattaatat
     5401 ttgtaaagcc acgttttgga atacgacgga acaatggtgt ttgtccacct tcaaaaccta
     5461 aacgtacacc gccaccgcta cgagcttttt gacctttttg accgcgacca gatgttttac
     5521 cattacctga tgatgtccca cgaccaacac ggttacgtac tttacgtgag ccttcagcag
     5581 cttttaattc atgaagtttc attttttctc ctttttttgt aaaatgctag cgcctctatg
     5641 agggaaaagg actccctata gaaactcgcc tatacattaa tgatacttaa gttatccaaa
     5701 ataacttaat acagatacgc actaaccgac tactgtttgt tgccaaaaaa gcaaactaca
     5761 agagtcggtt ttgtgctcgt ttttaattat gaaatcagag atttcacagt tataacttat
     5821 ttaacatctt caacggtaac caagtgagaa atagctgtca ccataccacg gatagcagca
     5881 ttatcttctt tgactactga agagtttaat ttaccaagtc caagagcaac aacagtttta
     5941 cgttgttctg gcttacgtcc gataggagac ttagtcaaag taattttaat ttgagccatg
     6001 atgatcccct ttcttatgct aagtcagaaa ctgagatgcc acgaagtgcg gcaacttctt
     6061 ctgcacgttt aagttgtttc aatccttcaa cagttgcacg aacaatgttg attggagtgt
     6121 ttgaaccaag agattttgaa gtaatatcag caacacctgc taattcgatg acggcacgaa
     6181 ctgcaccacc agcagcaact ccagaacctt ctacagctgg tttcaataat actttagctc
     6241 caccgaagtt agtgtaaact tcgtgaggga ttgttgtacc aaccattggt acttcgatca
     6301 tgtttttctt agcagcttca actgctttac ggatagcttc tggaacttct tgagctttac
     6361 cagtaccaaa tccaacacga ccatttccat caccaacaac tacaagagct gcaaagcgaa
     6421 gacgacgtcc acctttaaca acttttgtaa cgcggttaat cgcaacaacg cgttcttcaa
     6481 gttcaactgc attatcttta aatgccatta ttttgtgtcc tccctattag aatttcaatc
     6541 cgttttcacg agctgcatca gccaaggctt taacacgtcc gtgatagaga tatccaccgc
     6601 ggtcaaacac cacttcagaa atacctttag ccactgcacg ttcagcaaca agtttgccga
     6661 ctacaacggc ttgttctgtt tttgttcctt tagaaacgtc tttatcaaga gttgatgcac
     6721 ttgcgagcgt tacacccgct acgtcatcaa taacttgagc gtagatgcct gtattagaac
     6781 ggaatacgtt caaacgtggg cgatcagcag ttccagagag tttaccgcgg acgcgacggt
     6841 ggcgtttttg gcggattttg tttttatctg gtttcgaaat cacaattttc acctcttaat
     6901 tttaatcaat ttgtctcatt gacaagttgt cgattgaact tatcgagcat gttaaggtgc
     6961 aatacttccc atacccgact aagaacatta tttacctgtt ttaccttctt taaggcgtac
     7021 gtattcacca acgtaacgga tccctttgcc tttgtaaggc tctggtgaac gcaagctacg
     7081 gatgtaagca gctgtttgac caacaacttc tttgttgatt ccttcaactg agattgaagt
     7141 tgggttagca acagtgaaag taattccttc tggagcttca acttcgtctt ggtgagactt
     7201 acctactgaa aggacaagtt tagtaccttg aagttgagca cggtaaccga cacccttcat
     7261 ttcaagatct tttttgaaac cttcagaaac acctacaacc atgttattca agttagcacg
     7321 ggttgtccca tggattgttt tcatttcttt tgagtcgtta ggacgtacaa ctgtgatttc
     7381 agtcccttca actttgattt caatattttt gttgaactca cgagtgagtt cgcctttagg
     7441 gcctttaaca gtaataacat tgttgttatt tgttaattca acgcctgcag gcatagtaat
     7501 tactttatta ccaatacgtg acatgtttat tttctcctgt taaattgtca agccgcatag
     7561 cgactagttt tcacgggggt ttgatagaat gatatacttc aatttatatt tcaaaattga
     7621 acacgagctg tggtctgtgt gaaaaagata aatcttcctt gagtgtaaac acgctacgtc
     7681 agatttccta ttttcacttt gtccacgacg ctcttagtat cttaaattac caaacgtaag
     7741 cgatcacttc tccaccaaca ttcttttggc gagcttcttt gtcagtcaaa agaccttctg
     7801 atgttgaaat gattgcaatt ccaagtccgt taagaacttt aggcatgtca tcacgttttg
     7861 cgtaaacgcg aagacctggt tttgaaatac gttttaagtt agtgataaca cgctcaccat
     7921 tttttccgta ttttaagaac acacgaatga tgccttgttt atcatcttcg ataacttcaa
     7981 cattttttac aaaaccttca cgtttaagaa tctcagcaat ccctttttta atgtttgaag
     8041 caggtacttc taacacttcg tgttttactt gattagcatt acggatacgt gttaaaaagt
     8101 ctgcaattgg gtcagtcata accatttttt atattctcct cttactagca gtttgatgct
     8161 atcacttact agttaatgtt gtccttaaag gactttggta tcagcttatg ctcacaccgt
     8221 tttaattaag cacaagactt aatctgaaga acaaattaag ttttgtgtca tatttttacc
     8281 aagaagcttt gacaacacct ggaatttgac ctttgtaagc caattcacgg aagcaaacac
     8341 gacaaagttt aaacttgcgg taaactgaat gtggacgtcc acatttttca cagcgagtat
     8401 aagcttgtgt agagtgtttt gcaggacgtt tgttcttagc aatcatagat tttttagcca
     8461 atttatttac cccctattat tttgcaaaag gcattccaag gcccttaagc aattcacgtg
     8521 attcttcgtc agtatttgca gtagtgacga ttacaatatc aagaccacgt actttatcaa
     8581 catcatcaaa gctgatttct gggaagataa gttgttcttt cacaccgaga gtgtagtttc
     8641 cacgtccatc aaacgatttt gtcggaacgc catggaagtc acgtacacga ggaagtgaaa
     8701 cgctaaccaa tttgtctagg aattcgtaca tacgttcgcc acggagggta acttttgcac
     8761 cgatcgctac accttcacga agacggaagc cagcgattga tttcttagct ttagtaataa
     8821 gtggtttttg acctgagata agtgctaatt cagctgcagc tttctctagg ttttttgcgt
     8881 tggatacagc atcaccaaca cccatattga gaacaatttt ctcaactttt ggcacagcca
     8941 taactgatgt gtaattgaat ttctctgtca acgcaggaat tacttcgtta gtatattttt
     9001 cttttaaacg atttgccatt atgcttctcc tttccttcgt gattaatcaa gcacttcgcc
     9061 tgattttttg ctgtaacgta cttttttacc gtcaacaact ttataaccaa cacgtccagc
     9121 tacaccattt ttatctaata cttgaacatt agagacatgg ataggtgctt ctttttcgac
     9181 gatagctcct tgaggatttt cagtgttagg tttttggtgt tttttaatca ttccaacacc
     9241 ttcaacaata actttgttaa cttttgggag tgctttaaga actacagctt cagtgccttt
     9301 gtccttgcca gcaattacgc gaactttgtc gccttttttt acaaacattc tggtttctcc
     9361 tattatttct tacgccccga tgggcaccct aggaaaacct aggggactac gtttgttaat
     9421 ctgagattaa agtacttctg gtgcaagtga tacgatcttc atgtagccac cctcacgcaa
     9481 ttcgcgtgca actgggccaa agatacgagt tccgcgagga gttttatcat cacggatgat
     9541 tacagcagca ttgtcgtcaa atttgatgta tgaaccgtct ggacggcgtg caccagtttt
     9601 tgtacgaacg ataacagctt taaccacatc acctttttta actgctccac caggagtagc
     9661 ttgttttaca gaagcaacga ttacgtcacc gatgttagcg aatttacgtc ctgaaccacc
     9721 aagtactttg atagtcaaga tctcacgagc accgctatta tcagcaactt tcaagcgagt
     9781 ttcttgttga atcatttcaa ttttctcctt ttagtttgat tagataataa cagctttttc
     9841 cactacttcc acaagacgga aacgttttgt agctgaaagt gggcgagttt ccatgatacg
     9901 aacgatatcg ccttctttag caacgttgtt ttcatcatgt gctttatatt ttttagaata
     9961 gttgatacgt ttaccataga ctgggtggtt acgtttagtt tcaactacaa ctgtgattgt
    10021 tttgtccatc ttgtcagaca ctacgcgtcc gtaaagagtt ttacgttgat tacgttccat
    10081 ttagaatttc tcctttccca atctattatt tcatttctga ttgcacagtt ttaacacgtg
    10141 caatttgttt cttaacttcg tcaaggcgag cagtcttttc aagttgacct gctgcagctt
    10201 ggaaacgaag atcaaacaat tcttttttga gttcgttttc tttcttagca agttcttctt
    10261 gagacaatcc acgaagctct ttaacaaaat ctttaatttc ttgaagtttc atgtcttctc
    10321 cttattctgc ttcacgtttt acgaatttgc acttaactgg taatttgtgg ctagcaagac
    10381 gtaatgcttc gcgagcaatt tcttcagaaa caccagcaat ttcaaacatc actttaccac
    10441 gtttaactgg tgctacccaa ccttcaggtg cccctttacc agaacccata cgtacaccga
    10501 tagcttttgc agtgtatgat ttgtgtggga agattttaat ccaaacttta ccaccacgtt
    10561 tcatgtaacg agtcatcgca atacgggcag cttcgatttg acggtttgta atccagtggc
    10621 tagttgtggc ttgaagtccg tattcaccga atgaaacttc tttaccacct ttagcctcac
    10681 cgcgcatttt tccacggaat tcgcgacggt gtttaacacg tttaggtact aacatttgtt
    10741 atttgcctcc tttagtgttt ttacgagctg gaagaacttc tccacggtaa atccaaactt
    10801 taacgccaag tttaccataa gttgtgtcag cttcttccca agcatagtcg atatcagcgc
    10861 gaagcgtgtg aagtggaaca gttccttctg aataaccttc agcacgagcg atatcagcac
    10921 cgttcaaacg accagaaact tgagttttaa tccctttagc tcctgcacgc attgtacgtt
    10981 ggattgcttg tttttgagca cgacggaaag caacacgttg ctcaagttga cgagcaatgt
    11041 tttcaccaac aaggtgagca tctaaatcag gttgtttgat ttcgatgatg ttgatgtgta
    11101 cttgttttcc agttaatttg ttaagttgac cacgaagagc gtcaacgttt gcgccacctt
    11161 taccgataac catacctggt ttagcagtgt gaagtgaaac aataactttg tttactgcac
    11221 gttcaatttc aatagttgaa actgatgcgt cagctaattc tttattaatg aatttacgga
    11281 ttgcaagatc ttcatgaagg taatccgcgt attctttttc agcataccat ttcgcatccc
    11341 agtcacggat gattccgaca cgcataccaa ttggatgtac tttttgaccc acgattttac
    11401 ctccttattt ttctgataca actacagtta cgtgagttgt acgtttgttg attggtgaag
    11461 ctgaaccttt cgcacgtgga cggaaacgtt tcattgttgg tccttcgtta gcgaatgttt
    11521 cagatactac caagtttgct ttttccaaac caaagttgtt ttctgcgtta gcaattgctg
    11581 agttaagagt tttctcaata acacgagctg ctttgtttgg agtgaatttt aagattgcga
    11641 ttgcgtcagc aactttctta ccacggataa gatcaagtac taaacgtgtt ttacgaggtg
    11701 aaacacgcac tgtacgagcc attgctttag ctgaagtaat ttctgccatt gtgtcctccc
    11761 cctattaacg acgtgttttc ttgtcgtcag ctgcgtgacc tttgtaagta cgcgttggtg
    11821 caaattcacc aagtttgtga cctaccatgt cttcttggat gtaaacaggt acatgtttac
    11881 gtccgtcata aactgcgatt gtatatccga tgaaacttgg gaaaatcgtt gaacgacgtg
    11941 accaagtttt gattactttt ttcttttcgt cgtttgcttg agcttcaact tttttcatca
    12001 aatgctcatc gacgaaaggt ccttttttaa gactacgtcc cattttgtag ttttctcctt
    12061 taaataatgt accacagcgg cttgagttca agagcaagac tccaaaactc taccgagttg
    12121 gcggaataaa tagcaaagct attgtaacgc tatttcagca agttttaagc aacttactaa
    12181 gattattttt cgttacgacg acgaacgata agtttgtctg atttagcttt cttgttacga
    12241 gttttaagac caagcgctgg tttaccccat ggagttgatg gcgctttacg tccaactggt
    12301 gctttacctt caccaccacc gtgtgggtga tcgttagggt tcattacaga accacgaact
    12361 gttgggcgga tacctttcca acggctacgt cctgccttac caatgttaac aagtgattgt
    12421 tgttcgttac caacagtacc gatagttgca cggcatgtac caagaatcat acgaacttcg
    12481 cctgattgaa gacgaacaag aacgtattta ccttcttgac caagtacttg agcagaagct
    12541 ccagctgcac gaacaagttc tccaccttta ccaggtttca actcaatatt gtgaacaact
    12601 gtaccgacag gaatgtttgc taatggaagt gcgttaccaa ctttgatatc tgcatctgga
    12661 ccagaaacaa tacgttgacc tacttcaaga cctttaggtg caatgatgta agctttcaca
    12721 ccgtcagtgt aatgtacaag tgcgatgttt gcagtacggt ttggatcata ttcgattgtt
    12781 ttaacaactg cttcaacgcc atctttgtta cgtttgaagt cgatcaaacg gtaatgacgt
    12841 ttgtgtccac caccttggtg acgaactgtg atgcgaccat tgttgttacg accagctttg
    12901 ctcttaagag aaacaagcaa tgatttctca ggcgtgcttg ttgtgatttc cgcgaaatcc
    12961 aaagaagtca tgttacgacg gccatttgtc gttggtttat aaactttaat acccacgtta
    13021 tttcctcctt agattattca gcttcagctg cgaacaactc gattgcttta gaatcagctg
    13081 taagagtgat gatagctttt ttagtttttg aagtgaaacc tgtgtaacga ccaacgcgtt
    13141 ttgcttttgg tttaacgtta acagtgttta cacttgcaac tttaactccg tcaaaagcag
    13201 cttcaacagc ttgtttgatc aaaagtttgt gtgcacgagt atcaacttcg aaagtgtatt
    13261 tgcctgcttc aagagcaatc attgacttct cagtgataac tggttttttg attacgtcgt
    13321 acaaattcat tatgcaagaa cctcctcaat tgtagagatt gcttctttag taacaagaag
    13381 tttgtctgcg ttcacgatat caagaacact tgcagttgct gcagttgcaa cagttacgtt
    13441 tggaaggtta cgtgcagaaa gtgctgcaaa ttcatttcct tcttcaacaa gaacaagtac
    13501 ttttgtatca atgctaagag ctgaaagcac ttttgcaaat tcagcagttt ttggtgctgc
    13561 aaatgaaagg ccttctacag ctacaaattt atcttcagca acttttgctg agtaaactga
    13621 tttcaacgca aggcgacgaa ctttttgtgg aagtttgtat ccgtatgaac ggggagttgg
    13681 tccaaagaca acaccaccac cacgccattg tggtgagcgg atagaacctt gacgagcgcg
    13741 tccagttcct ttttgacgcc atggtttacg tccaccacct gatactgctg aacggttttt
    13801 aaccgcatga gtaccttggc gaaggctagc gcgttggcta attacaacat caaaaacaac
    13861 tgattcgttt ggttcgatac cgaagatagc gtcgtttaat tcaactgagc taacttcttt
    13921 accagtttgg tcaaatagtt ttacgtttgc cattttaact gttttctcct ttcttattat
    13981 ttagcagctt taactgctga cttgatagtg ataagagatt tcttagcacc tggtacgtta
    14041 cctttaataa ggataacgtt cttttctggg ataacttgta caatttcaag gttttgaact
    14101 gttacacggt taccacccat acgtcctgcc aagcgtttgt ttttgaaaac gcggttaggc
    14161 gcaacaggtc ccatagaacc tgggcgacga tggtaacgag aaccgtgagc cataggacca
    14221 cgtgattgac catggcgttt gataacacct tggaaacctt taccttttga tgtccctgtg
    14281 acgtcaacaa catcaccagc ttcaaattgt tctacagata attctgcacc aacttctaag
    14341 ccttcaatgt ttttgaattc acgaatgaag cgcttaggag ctgtgtttgc ttttgcaaca
    14401 tggcctttgg caggtttgtt actcaagact tcacgtttgt catcaaaacc aacctgaact
    14461 gcttcataac cgtctgtttc aacagtttta acttgaagca caacgtttgg agttgcttca
    14521 atgacagtaa cagggatgaa ttcgcctgat tcagtgaaga tttgagtcat tcccactttt
    14581 ttccctaaga ttccttttgt catgagaaaa tatttccttt tcttaataat atttcaaaaa
    14641 gttttttatg agcgtttttt gtgctcaaaa aagtttttta cgagcgtttt ttgtgctcat
    14701 agcaagatac aaagagcgtc aaactcaaaa taaaaataga aaatctaaca caaaaacttc
    14761 tgtttcgagg aagatttatc tttttcatgt agagtttagc tcgtgttcaa gttccatcaa
    14821 acactaactg tacttgcaaa atctcaccga ttaaagtttg atttctacgt tgacaccact
    14881 tggaagatca agtttcatca aagcatcaac agttttttgt gttggattga tgatgtctac
    14941 caaacgtttg tgtgtacgca tttcaaattg ttcgcgagaa tctttgtatt tgtgagtcgc
    15001 acgaataatt gtgtaaagac tgcgttcagt tggaagtgga actggtccag caactgtagc
    15061 acctgtacgt gttgcagttt caacgatttt ttctgccgct gtatcaagtg tacggtgttc
    15121 gtacgctttc aaacggatac ggattttttt gtttgccatc ttttctcctc tcgtctattt
    15181 acgataatag gctagctcct caagaaaacc gacacctgtt gcgtggcaat gcaaccgagc
    15241 gtgtcgcaac ctcttgtatc attgctaaag ctgtattttt acagcaccac aatataataa
    15301 cagaatcatt tagtcattgc aagcttttta gggcattttt tcaaaaaaac taccttccta
    15361 atgaaaggta atttcttggt actttttaaa ttgtttagag agaataaacc accgactggt
    15421 attatctaac actgtcgcca tccagacccc tatgagtcca tattgccaaa ccacaccaat
    15481 cacataacca aggccaatgc gaatcaccca cattcctatg gttgttgcat agaagggaag
    15541 ctttgctttt cccaaaccct gccagacggc tgtataaact aaagtgcctg ccgttgcggg
    15601 agctcctagt aaagaaaaaa gtaaaacaac catggcggac ctttgagcat cagtattctg
    15661 agtaaatagc ggcaagagac taggacctag cagataggtc aaggctccca taaccagcat
    15721 catgagggtg gataaaataa aagcttctct aataatatac cttatctcag tcaccttacc
    15781 gccccccagt tgcctggcca ctagaataat tgttgccgtt gccatggcta agccaggcat
    15841 gtaattaaac tgagtcaagg tttctccaat agcattacct gccaaggccg ttgtcccaaa
    15901 acgaacaaca atgccaataa ttaggacatc tcctgctctc atcatcaaac gttcaccagc
    15961 agcaggtagt gataaatcaa aaataatttt gtccaaaggt ctcatcaatc gtttcgcaac
    16021 ctgtttaata ggaataaact gacaaagtaa aaacacaccc actaaacgag ataaaaccgt
    16081 cgcccaagca acaccaagaa gaccaaatcc ccatacatag atagacaagg cagaaaaaat
    16141 ggcattgagt acgtttatta ataagctcac ttgcatagga atcttaggat acccttgagc
    16201 tcgaacaata gctcctaagc tagttaaaag tcctagacta acaatcatac cgccgacaat
    16261 ggatagatat tgccctccaa ccaatgttac agaagcttca gctcctagcc attctaacac
    16321 ctgatgatgt ccaacaactg ataacaaccc taagccgacc gaaaggagga gtgttacttg
    16381 caagacacct gccatatagt ttaattgttt gttctgatta ttttctccaa tacttcgagc
    16441 aatcagacta gatacagcgg ctcccagcgc gataaagagg gactgataaa tactgataat
    16501 gttgttagct atggagacac cagagactgc tacaagtcca atttgagcca ccaggtaatt
    16561 atcaaccatt cccattaaca tctgtaaaat attttctatc atagaaggca gagctaaaga
    16621 gaaaattttt cttctattat atatcattta tgtcccctaa taaaaaattc ttgatcaaac
    16681 gattttttta aacacagtca ctggactcat tcagttattt tgttccatta atgaattcat
    16741 aaagtctacc ttaatagcag agtcccaaga tacacgacac ttagtgttac agtaacaatt
    16801 gaccacatgc caatccttaa catttaccta acgtttataa ctaagaagac gaagaacaaa
    16861 aggactccaa acgaattttt tctattatat aatatattct tcatttagca actttcaatg
    16921 tatcaaaata gcactctcga gtcaaaaatg ttttctgata ctcgagagtg ctattttatt
    16981 tggaatcaag tttaagcaac ttaggcttat ctcatattta atacatttgt atgtttagct
    17041 gatgaaatct aagaccttgc gcccttggat taaaccacgt tccatttcat caaagacttc
    17101 tggagcagtg tcaactggaa ctttttcgac aaccggagca accagacctt ctgcaccaaa
    17161 ggcaaaggct tcttccaagt ctttacgtgt ccctacaagt gatccgacca ctttgatacc
    17221 atctaaaaca gtttttacga tagacagttc catgtattct gatggaagac ctacggctac
    17281 aactgttcca ccggcacgga cactatcaat tgcttggtta aaagcaactt tagaaacagc
    17341 cgttaccaca acaccatgtg ctccaccggt tttttcttga atatagcctg gaacatcttc
    17401 aatttctttg ccgttaacta aaatatctgc gccaacttct ttagctaact caagtttatc
    17461 attattaatg tccactgcga caacatgtgc attaaagact tttttagcgt attgaacagc
    17521 cagatttcca aggccacctg ccccaaagat aacaatccat tggcctggag cagcacctgc
    17581 ctctttaata gctttataag ttgttacacc agcacaagta atggaagaag cttgagccgg
    17641 gtctaaacct tctggaactt taactgcata atcagctgtt actactgcat attcactcat
    17701 tcctccatca acactgtatc cagcattttt aacactgcga caaagtgtct cacgtcctgt
    17761 agtacagtat tcgcaatgac cacatccttc aaagaaccat gcaatagaga cacgatcacc
    17821 aacctttaag gatgttacgc cttctccaat ttcttctact ataccaatac cttcgtgccc
    17881 caaaatacgt cctggtacct gaccaaaatc gccatgtgca acgtgtaaat cagtatgaca
    17941 aacgccacag tattctactt taacaagcgc ttcaccatga ccaacatttg gaagatcatg
    18001 ctcaacaact tcgacacctg tactagcttg attaacaact acggctttca tatcagtcct
    18061 ccatctaata acgacttatt tgtaatcggt tacaaataaa ttgtacatct tttcacaatt
    18121 aaagtcaaca gaaacctgaa aagtttctga aacttaactg gttaatctta tagctgtttg
    18181 cctatctgca aaatacttaa ctacattttc tgacactgga tataaatgat atatttagaa
    18241 aataaaatga tttcttatga ctttaatcat aagaaatcat ttcctggtat caacattaaa
    18301 aatcacttac gtcgtcctgg acgttcagca taaccatagt aagcatcttc aataatttct
    18361 tgcatgtgat caaccattgg aagacgtggg ttagcaggtg aacattggtc ttcataagca
    18421 aggtaagcca attcacgaga atgttctttc cattcatttt cgtcgatacc ttgagctttg
    18481 aagttcattt gaataccaac tcggcaaccc agatcataaa cagctttcgc ataagattcc
    18541 actgcttctt ctggtgtaga agctggaagc cctaacaatt tcgcaatgtc ttggtatttt
    18601 tcatccgcac ggtagtaatt atacttaggc catgtcgccg ttttagctgg acgagttcca
    18661 ttatagcgga taacatacgg caaaagaatt gcatttgtac gtccgtgtac ggtatggaat
    18721 tgtgcgccaa ttttatgcgc cattgagtga gaaataccta agaatgcatt ggcgaaagcc
    18781 atacctgcca tagttgacgc attgtgcatc ttttcacgtg cttcaaaatc ggctatttta
    18841 actgattttt caagattatc aaaaacaatt ttaatggctt gaagagctaa accatccgta
    18901 aagtcattgg ccatttgtga cacgtaagct tcggttgcgt gtgtcaaaac gtccatacca
    18961 gtatcagcag caataaagcc tggaacagtc aataccaaag ctgggtcaac aatggctaca
    19021 gttggtgtta atgagtaatc agcgattgga tatttacgat tatttgcttt gtcagagata
    19081 acggcaaatg gtgtcacctc tgaacctgtt ccagaagttg ttgggatagc aacaaatttt
    19141 gttttcttac caagttctgg gaatttgaac gcacgtttgc ggatatccat gaatttttga
    19201 acgaggtcat ggaaatcaac ttctggttgc tcgtagaaga gccacataac tttggctgca
    19261 tccataggag atcccccgcc aagggcaata atggtgtctg gtttgaaggt acgcattaat
    19321 tcagtacctt tcataacagt tgtaatgtct ggatctggtt ctacttcagc aaagatctga
    19381 taaacaactt tattgcgacg aagatcaagt tgttcaatga tacgatccaa gaaaccaagc
    19441 tcaaccatag cgtgatctgt aacaatcatg acacgttcaa cgtcacgaca tttttgcaaa
    19501 tattgaattg aatcacgttc gaagtatgtt tttgaaggaa ctttaaacca ttgcatatta
    19561 tttctacgtc ttcctacttt tttgatgttc aagagattta tagcactaac attatcacca
    19621 actgcgttac gtccatacga accacaacca agagtcaatg atggcaagaa agcattgtaa
    19681 acatccccga taccaccaaa tgtagaaggt gaattccaga tgacgcggat agcacggatt
    19741 ctagttccaa attcttttgc caactctgca tctgctgtat gaatggcagc tgagtgaccg
    19801 agtccattaa attcaaccat ttgacgagct ttttcaacac cgtcttcacg tgattcagat
    19861 ttcaaaacag caatgactgg agataatttt tcacgtgtta aaggctcatt ttctgaaact
    19921 tctttacatt ctgcagcaag aatattggtt ccttctggaa cagtaaatcc agcttgttct
    19981 gcaatccaag tagctggttt tcctacaata tttggattta attttgcacc agcacagttt
    20041 ttgctatttg ctttggcacc aaaacagaat tcttcaagaa gggctttttc ttttttattt
    20101 acaaaataag tatggtaaga tttgaattct gctacaaaat cgtcgtagat ttctttatcg
    20161 atgataacag cttgttcaga ggcacagacc ataccattat caaatgattt agacatcaca
    20221 atatcatgag cggcttgacg gatattagct gatttttcaa cataagctgg aacgttacca
    20281 gcacctaccc caagggcagg tttcccacat gagtatgccg ctttaaccat cgcatttcca
    20341 cctgtcgcaa ggatagtggc aatgccgtca tgattcatca aagcatttgt tgcttcaagt
    20401 gatggtgttt caacccactg cacacaattt tcaggagcac cagcggcaat agcagcatca
    20461 cgaacaatac gagcggcatg tgctgatgat tcttgtgctg atgggtgaaa ggcaaaaata
    20521 attggattac gtgtttttaa cgagatcaaa gatttaaaga tagctgttga agttgggtta
    20581 gttgtgggtg taatcccaca gataacaccc accggctcag caattaatgt caaacctgtc
    20641 acatcatctt cttcaataat tcctactgtt ttttgatggc gcatgttatt aaccacatgc
    20701 tcacaagcaa atagattttt tgttgcttta tcttcaaaga caccgcgacc tgtttcttca
    20761 taagcatgtt tagcaagttc accgtgagca tccaaggcag caacagaagc tttggctacg
    20821 atataatcaa cttgttcttg gtcaagctta cgcatctctt caagagcagc taaacctttt
    20881 tgaacaagag cattaattgt cacagaaaca gaggttgttt caacagtatt atgtccttca
    20941 gtcatttgag actcctttat cttgttaaaa gtttcacaat tttgtt
//
LOCUS       DATUYR010000028        20323 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.028, whole genome shotgun sequence.
ACCESSION   DATUYR010000028 DATUYR010000000
VERSION     DATUYR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20323
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.028"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..859)
                     /locus_tag="VCI33_000997"
     CDS             complement(<1..859)
                     /locus_tag="VCI33_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2831930.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSIITF
                     NNSNYAKLIH"
     gene            1102..2349
                     /locus_tag="VCI33_000998"
     CDS             1102..2349
                     /locus_tag="VCI33_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP2831931.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQILFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            2623..3984
                     /locus_tag="VCI33_000999"
     CDS             2623..3984
                     /locus_tag="VCI33_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2831932.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPNFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINAKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            3984..4820
                     /locus_tag="VCI33_001000"
     CDS             3984..4820
                     /locus_tag="VCI33_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2831933.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            4978..5775
                     /locus_tag="VCI33_001001"
     CDS             4978..5775
                     /locus_tag="VCI33_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2831934.1"
                     /translation="MTSKITIKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHI
                     SNLLNYTPNQAARALVTQQSNSVVLYYKYADSILARAEQLHTISFLSRLLHEQDYQLV
                     CLNEDSIEKFDWADLILTLDVDNDTFYKIGKLNFKPLIAIDSFVNDPLFFQINTDYAA
                     LKDQANQFFKDEPYTLLSLPITNRERYEHVSAFFPSLHIVNDLRELTYFEKENLLLID
                     ESLDQFFVNSSQKLYCPRFSPKKGQVILEAIEWAKKRQPDKKHNLLV"
     gene            complement(5891..6706)
                     /locus_tag="VCI33_001002"
     CDS             complement(5891..6706)
                     /locus_tag="VCI33_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEP2831935.1"
                     /translation="MPDKKQFPISYISHCLSARAMFASRAQFTWWQNLLIIVFLNALI
                     MIPVTLHYANMTTYPLERIVTKSLSPITDKTYQALTQGKIEKDTFQGQSLIRRDGELV
                     LAVLPTKVDLEQLASESTRQIIVTKKEWRFVTPDGKELRAHVRGQQQSLADLTTVKAV
                     KDFVNQQWYDSNKASVLGFLLLTFVLMVCVGTLIVIGLGAFFLTLTKRSRLFMIRNFS
                     EGLGLMVNCLAWPSLLAIALSFFIQDPVLIMNCQVFGTLLMLTWVFYKTQFRD"
     gene            complement(6728..7588)
                     /locus_tag="VCI33_001003"
     CDS             complement(6728..7588)
                     /locus_tag="VCI33_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2831936.1"
                     /translation="MRKTYVSISMKRKRFFTQLLTYLYLISLVIVILFPILVTVSSAF
                     RPGNTTAFSFHFDGPWTLSNFKTLFQDTLYLRWYWNTLIVAFFTMLIQVTVITLTGYA
                     YSRYNFFGRKKSLIFFLVVQMVPTMAALTAYFVMAWLFNALNQYWFLILIYVGGGIPM
                     NAWLMKGYFDTVPYDLDESAKLDGSGHFRTFYQIVLPLVRPMIAVQSLWAFMGPFGDF
                     MLAKFLLRAQENYTVAVGLQSFITNDARNPKVTLFAAGAILIAVPISVLFFFLQKNFV
                     SGLTSGGTKG"
     gene            complement(7585..8892)
                     /locus_tag="VCI33_001004"
     CDS             complement(7585..8892)
                     /locus_tag="VCI33_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP2831937.1"
                     /translation="MVDNQRQQKPNKAMWLSVIPGLGQLYNKQIVKGGVLLVVFFLEL
                     LEIVVLGIPALTGFYSLGSVPMQDHSLFMLIKGAMQLITLVLFGIFHLVAMRDAKLVA
                     HQMNEGKKVPVTAKETLEAIYEKGFPYLLIIPAYLAMAFAIIFPVLVTLLIAFTNYDF
                     RHIPPYRLLDWVGLKNFLNILELSTFKTAFTSVLSWTLIWTLAATSLQIVIGIATAVI
                     ANQSFIRGKRLFGVIFLLPWAVPAFISIMSFGNFFNDSIGAMNVQVLPFIEKFLPFVD
                     FGIVSWKTDPFWTKVAVIMVQGWLGFPYIYILVSGILQAIPADLYEAATVDGATVLQK
                     FRHITMPMIFAVAAPTFISQYTFNFNNFSIIYLFNNGGPGTVGGGAGTTDILISWIYK
                     LTTQTSPQFSMASAVTLIISVIVISVSLITFKKFKAFDMEERS"
     gene            complement(8967..11102)
                     /locus_tag="VCI33_001005"
     CDS             complement(8967..11102)
                     /locus_tag="VCI33_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrin glucanotransferase"
                     /protein_id="HEP2831938.1"
                     /translation="MRELHIKTYKLLTKSAVLLGLISFPLTVSAADNASVTNKADFST
                     DTIYQIVTDRFNDGNTSNNGKTDVFDKNDLKKYHGGDWQGIIAKIKDGYLTDMGISAI
                     WISSPVENIDSIDPSNGSAAYHGYWAKDFFKTNQHFGTEADFQQLVKVAHQHHIKVVI
                     DFAPNHTSTAEKEGTTFKEDGALYKNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYH
                     SMYGLADLNNINPKVDQYMKEAIDKWLDLGVDGIRVDAVKHMSQGWQKNWLSHIYEKH
                     NVFVFGEWFSGHTDDDYDMTTFANNSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVL
                     ENRDQVTNEVTDQVTFIDNHDMERFATKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ
                     YATGDKDPNNRGDMPSFNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFE
                     RKFGNHVALVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSF
                     ELGAGQVAVWTYEGEDKTPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEI
                     LSWSDTLITLKVPTVPANYYNISVTTADKQTSNSYQAFEVLTDKQIPVRLLINDFKTV
                     PGEQLYLMGDVFEMGANDAKNAVGPLFNNTQTIAKYPNWFFDTHLPINKEIAVKLVKK
                     DSIGNVLWTSPETYSIKTGHEAQTITIKK"
     gene            complement(11128..12831)
                     /locus_tag="VCI33_001006"
     CDS             complement(11128..12831)
                     /locus_tag="VCI33_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEP2831939.1"
                     /translation="MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGP
                     KYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTT
                     SYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
                     LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQF
                     GTKYDLQELIDLAHQMGIKIILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEH
                     PSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHD
                     FWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTS
                     KQLAEKLSNILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGI
                     PCLYYGTEVAMEGGYDPDSRRGFPWDDAERDKDFFSKVKAIIALRQQPALQEGAISID
                     YDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQDGYIVFS
                     N"
     gene            complement(12997..14256)
                     /locus_tag="VCI33_001007"
     CDS             complement(12997..14256)
                     /locus_tag="VCI33_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP2831940.1"
                     /translation="MSWNWKKASVLGTLSLASVLPLTACVSGGGKGVKETDGKTIVVS
                     VDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRV
                     GGLGTEGQISEVTLGNSKEFDDTVKKLVTIDGKTYGAPDVIETLVTYYNKDLVPQAPK
                     SFTELEVLQKDSKFAFASEPGKSVGFLAKWTDFYYGYGLIAGYGGYIFGDKGTKPSDL
                     GLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASSFK
                     DAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY
                     DKTQEIPANLTARNYASKEGNELTKAVISQFESAQPMPNIPEMAEVWEPGANMFFNVA
                     SGKEEASKAAKEAAKTIKEAIEQKYAE"
     gene            complement(14865..15848)
                     /locus_tag="VCI33_001008"
     CDS             complement(14865..15848)
                     /locus_tag="VCI33_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP2831941.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGIQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTALQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDCEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEPT"
     gene            complement(15879..17129)
                     /gene="dltD"
                     /locus_tag="VCI33_001009"
     CDS             complement(15879..17129)
                     /gene="dltD"
                     /locus_tag="VCI33_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEP2831942.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(17122..17361)
                     /gene="dltC"
                     /locus_tag="VCI33_001010"
     CDS             complement(17122..17361)
                     /gene="dltC"
                     /locus_tag="VCI33_001010"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEP2831943.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(17379..18635)
                     /gene="dltB"
                     /locus_tag="VCI33_001011"
     CDS             complement(17379..18635)
                     /gene="dltB"
                     /locus_tag="VCI33_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEP2831944.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(18632..20170)
                     /gene="dltA"
                     /locus_tag="VCI33_001012"
     CDS             complement(18632..20170)
                     /gene="dltA"
                     /locus_tag="VCI33_001012"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEP2831945.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGEHRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELASGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(20182..20322)
                     /locus_tag="VCI33_001013"
     CDS             complement(20182..20322)
                     /locus_tag="VCI33_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEP2831946.1"
                     /translation="MKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIYN
                     EF"
BASE COUNT         6507 a         4136 c         3408 g         6272 t
ORIGIN      
        1 ggtgaattaa tttagcgtaa ttagaattat taaaagtgat aattgacata tcatctggca
       61 cttttatatc ataaaaagat aataactgta ccatacgaat agccagaaca tcaccaacaa
      121 caaccaaggc agttgcttta aaggaagata tagtttccat caatatttct acgctaacag
      181 gatccttacg atcaaataac agcattggct tcgtttttaa agatcgctta aaacaaccct
      241 tcaaatagcc aagataacgc tcagaagtta cttctgatag taaatcatct gttataaata
      301 atatgttgtg atggcctttt tgataaaggt gttctactgc tgttttagcc attagctgat
      361 tatcattatc aatataagtg gttttattag tgtcaccttg tggagcacct acaatgacaa
      421 atggtaagtt atttgtcatc aaatattttt tgacaggatc atcttgatca gaataaagaa
      481 taataaaacc atcaactcgt ttttgaagat gcatcagttt cacttgctct atcaggtcat
      541 caagagatat acctgtagca atcgacacag taaaatgaga ttctttagct tcgttagtaa
      601 tagtcgacaa aatttccata aagaaaggtt cacttaatct atcgctagtt gtaataggag
      661 gaaaaacaag tcctatattg tgtgtcaaac cactcgctag aatctgtgct gcgacatttg
      721 gaacatatcc caaatctgcc atcgcttttc taaccttctc ttttgttttc atagaaatag
      781 atcgattatc ttttaatacc ctactcaccg tcgaagggtt aacacctgcc ttttgagcga
      841 catcttttat tgttaccata ttaacctcgt tactatattt accaaagata aaccttatca
      901 tgggtttgtc atgtatttcg tttccattat acaattatct ttcaaaaaat caacttcaat
      961 caagattccc ttgtcatttt aactcaaatt ctctaaacta tttttaaaaa aaacgacctt
     1021 tttttaaaaa aaggcttgac tttttctgca agcggttgca taatattaag tgtaagaaat
     1081 ataccttagg aggaaatagc tatgaaatca tggcaaaaag ttatcgtcgg cggagcaagt
     1141 ttgacacttg caagtacctt gttagtggga tgtggatcag gttcaaaaga taaaaaggaa
     1201 gctggcgctg actcaaagac tattaaactt tgggtaccga ctggctctaa aaaatcatat
     1261 gcagatacca ttgcaaaatt tgaaaaagat tctggttaca ccgttaaagt ggttgaatca
     1321 gaagatccaa aagcacaaga aaaaatcaaa aaagatgcct caactgctgc cgatgtcttt
     1381 tcacttcctc atgatcaact tggtcaactt gtagaatctg gtactattca agaagttcca
     1441 gaaaagtaca ataaagaaat tgctgctact tctactgatc aggcgctcgt cggtgcacag
     1501 tacaaaggga aaacatatgc tttcccattt ggtattgaat cacaaattct tttctacaat
     1561 aaatcaaaat tagctgctga agacgttact tcatatgata caattactac aaaagcaact
     1621 tttggtggta cctttaaaca agctaacact tacgctactg gtccattatt catgtcagtt
     1681 ggtaacactt tatttgggga aaacggtgaa gatgtaaaag gtactaactg gggcaatgaa
     1741 aaaggcgctg ctgttcttaa atggatcgca gaccaagctt caaataaagg ttttgttagc
     1801 ttagatgcta acaacgttat gtctaaattt ggtgacggtt cagtagcttc ttttgaatca
     1861 ggaccgtggg actatgaagc tgctcaaaaa gctatcggta aagaaaacct tggtgttgct
     1921 atttatccaa aagtaactat tggaggcgaa acggttcaac aaaaagcatt cttgggtgta
     1981 aaactttacg cagtcaacca agcaccagct aaaggtgata caaaacgtat cgcagctagc
     2041 tacaaattgg catcctactt gactaatgct gaaagccaag aaaaccaatt caagactcgt
     2101 aacattgtcc cagccaacaa agaagttcaa tcttcagaag ctgttcaatc aaacgaactt
     2161 gctaaaaccg ttatcactat gggttcttct tcagactaca ctgtcgtaat gccaaaactt
     2221 agccaaatgg gcacattctg gactgaaagt gctgctattc ttagtgatgc cttcaacggt
     2281 aaaatcaaag aaagcgacta ccttactaag ttgcaacaat tcgacaaaga tatcgctgca
     2341 acaaaataat atgatctcat gtcatcactg ctatactaaa acaagcacaa ggtaagtact
     2401 tctaaaaagt tctttccact taccttatga agagtggaac taggctccat cacattttat
     2461 tcaagcttag tctcactctt acttgttttt gttttactag tagcttagtg ctacttaaat
     2521 ttttaactct ttaaaagttt acgtaacact tagctatttc atcctaacaa gagattgaat
     2581 ctgaactacc actctagtat cctaaaataa aggagaatct atatgactca gtcaaacttg
     2641 acaccaaata tatctgtaat agaagcttta aaaagaggca gttgggatat taaactttcg
     2701 agtattatca tgggatttgc caactttgct aacaaacagt ttatcaaagg aatcttattt
     2761 ttaataagtg aacttatctt tctagtagct tttgtctccc aaatcatccc tgctatccgt
     2821 ggccttgtca ctcttggtac tcaaactcag ggaatgacta caaaaactat tgatgggatt
     2881 aacatccaag ttgctgttga tggcgataac tctatgctaa tgcttatttt tggtttagca
     2941 tcactcattt tctgcctagt ctttgcttac atttactggt gtaatctcaa aagtgctcgt
     3001 aacctttatc ttttcaaaca aaaaggtcag aagataccaa acttcaaaga agatcttgcc
     3061 acacttacaa atggtcgctt tcatatgact ttaatggcta tcccactgat tggtgtgctt
     3121 ttattcacca ttcttccttt gatttacatg atctgtttgg cctttaccaa ttttgatcat
     3181 aatcacttgc ctccaaaatc tttatttgat tgggtgggac ttgctaactt tggaaatgtt
     3241 ttaagcggcc gtatggcagg aacctttttt cctatcttct catggacttt gatttgggct
     3301 gtttttgcca cagtaactaa tttcttcttt ggtattattc ttgctttgtt aatcaatgcc
     3361 aaaggattaa agtggaaaaa aatgtggcga accatctttg ttatcaccat tgcagttcca
     3421 caattcatct cattattgat tatgcggaac ttactcaatg acgaaggccc actaaatgct
     3481 cttctcaata aaatcggctt gattaatggc tcactgccat ttttatctga tcctctttgg
     3541 gcaaaattct caatcatttt tgtcaacatg tggatcggta ttccatttac catgttgatt
     3601 gcgactggta ttatcatgaa ccttccaagt gaacaaattg aggctgctga aattgatggc
     3661 gctagcaaat tccaagtctt caagtctatc acattccctc aaattctctt gatcatgaca
     3721 ccaaacttga ttcaacaatt tatcggaaat atcaataact ttaacgtcat ttacctcctt
     3781 actggtggtg gtccaacaaa ttcagaatac tatcaggcag gaacaacaga cttgctggtc
     3841 acttggctct ataaattaac cgtcacagct gctgactaca atttagcttc tgttatcggt
     3901 atcttaatct ttacagtttc agctatcttt agcttacttg cttatacaag gacagcatcc
     3961 tacaaggaag gagcggctaa ataatgaaaa acaaacgtcg atttcaactt ggacttgttt
     4021 atgctacttt aatcatcctc tcaatcattt ggctcttccc aatcgcttgg gttattctta
     4081 ctagcttccg tagcgaagga actgcttacg tcaattattt tattcctaag acttttacat
     4141 taaatcacta cattaatctt tttactaatg aaacctttcc ttttggaaaa tggtttatga
     4201 atactcttat tgtagccact tttacatgta ttatttcaac cttcataact gttgccatag
     4261 cctactctct tagccgaatt aagtttaagt ttcgcaatgg ctttttaaaa ctagccctta
     4321 ttttaaatat gttccctggt ttcatgagca tgattgccat ctactatatc ttaaaggcct
     4381 taggtttgac gcaaacccta acagcacttg tccttgttta ttcctcaggt gcagcccttg
     4441 gtttttacat tgcaaaagga ttttttgata ccattcctta ttcacttgat gaatcagcta
     4501 tgattgatgg cgctactcgt atggatattt tctttaaaat aacccttcct ttagcaaaac
     4561 caatcattgt ctatactgcc cttcttgctt ttatggggcc ttggattgac tttatctttg
     4621 cacaagttat cttaggtgat gctacaagta aatataccgt tgctattgga ctattttcaa
     4681 tgcttcagcc agataccatt aacaactggt ttatggcctt cactgctggt tctgtcttaa
     4741 ttgccgtccc aatcactctc ctcttcatgt ttatgcaaaa atactatgtt gaaggcatca
     4801 caggtggttc tgttaaataa gttacaacat accaaacctt ctaagggcaa gccctttagg
     4861 aggtttttga taaataaggc cgtagataat catattagtc ctcacattta tcttcagatc
     4921 agccaaatgt attaatgatt ccttgacttt taccatgaat tcggatataa caaaatgatg
     4981 acatctaaaa ttaccattaa agatattgca cgagaagccg gggtttctgt ggcgaccgta
     5041 tcttacgtgt taaaccagcg caatgatcaa aaaatcagcg aagaaacccg caataaagtg
     5101 ctacatatta gtaaccttct taactataca cctaatcagg cagccagggc attagtcaca
     5161 caacaatcaa acagtgttgt cctgtattac aaatatgcag atagcatttt agctagggct
     5221 gaacaactgc ataccattag ctttctatcc cgcctattgc atgagcagga ctatcagctg
     5281 gtttgcctaa atgaagatag cattgaaaaa tttgactggg cagaccttat tctgaccttg
     5341 gacgttgata atgatacctt ttacaaaatt ggaaagctta atttcaaacc cttaattgcc
     5401 attgatagct ttgttaatga tcccctcttc tttcaaatta atacagatta tgctgctctc
     5461 aaagatcagg ctaatcagtt ttttaaggat gaaccttata ccctgctttc cttgcctatc
     5521 actaatcgtg agcgatatga gcatgtttct gccttctttc caagtttgca cattgtaaac
     5581 gacctaagag agctaaccta ctttgaaaag gaaaatcttt tactcattga tgagagtttg
     5641 gatcaattct ttgtgaactc ttcgcaaaaa ctctactgcc cacgtttttc cccaaaaaaa
     5701 gggcaagtta tcttagaggc catcgaatgg gccaaaaaac gccagcctga taaaaaacat
     5761 aaccttctgg tgtaacgcct gagaattaac ctatcttgac taattccttg taactcaaaa
     5821 ccttgcctct ttagataaag aagcaaggtt ttttgatgtc taatctaata acagggcctg
     5881 acttgtctca tcaatctcta aactgtgttt tgtagaacac ccaggtcagc attaataagg
     5941 taccaaatac ttggcaattc ataatcagta ctggatcctg aataaagaaa ctaagagcaa
     6001 tcgccagtag actcggccaa gccaaacaat taaccatcaa tccaagtcct tcagagaagt
     6061 ttcgaatcat aaatagccgt gatcgtttag tcagagtcaa aaagaaagcg cctagaccaa
     6121 tcacaataag ggtacccaca cacaccatga gaacaaacgt cagcaaaaga aaaccaagta
     6181 cactggcttt attactgtca taccattgct gattgacaaa gtccttcact gctttaactg
     6241 ttgtcaggtc agcgagtgac tgctgctggc ctctaacgtg agcacggagt tccttgccat
     6301 caggggtaac aaaacgccat tctttttttg tcacaataat ttgtctggtt gattctgatg
     6361 ccaattgctc taaatcgacc tttgtcggta aaacagccaa aaccaattca ccatctcgtc
     6421 gaatcagaga ttgaccctga aacgtatctt tctcaatctt tccttgggtc aatgcttgat
     6481 aggttttgtc tgtgataggc gacaaactct tagttacaat ccgttccaag ggatacgttg
     6541 tcatgttagc atagtgtaac gtaactggaa tcattattaa ggcgttcaaa aaaacaataa
     6601 taagaagatt ttgccaccaa gtaaactgag ctcggctggc aaacattgcc ctagcagaaa
     6661 ggcaatgtga aatgtaagaa ataggaaatt gttttttatc tggcattaaa cattactcct
     6721 tgtcaggtta ccctttggta ccaccacttg ttaaaccgga aacgaaattt ttctgtagga
     6781 agaaaaagag aactgaaata ggcactgcaa tcagaatagc tcccgcagca aataaggtta
     6841 ccttgggatt tctagcatca ttcgtaataa acgattgaag accaactgct accgtataat
     6901 tttcttgcgc ccttaaaagg aatttagcca acatgaagtc tccgaaagga cccataaaag
     6961 cccataagga ttgtactgca atcattggtc gcacaagagg aaggacaatt tggtagaagg
     7021 tcctaaagtg tccagaacca tctaacttag cagattcgtc caagtcataa ggtaccgtgt
     7081 caaaataacc cttcatcaac caggcattca taggaatacc accaccgacg taaatcaaaa
     7141 tcaagaacca gtattggttt aaggcattaa agagccacgc cataacaaag taagccgtta
     7201 aagccgccat ggtaggaacc atctgtacga ctaggaaaaa gattaaactt tttttgcgtc
     7261 caaagaaatt gtaacggcta tacgcataac ctgtcaaagt gatgactgtt acttgtatga
     7321 gcatggtgaa gaatgccacg atcaaggtat tccaatacca tcttaagtat aaggtatctt
     7381 gaaagagtgt tttaaagtta gacaaggtcc atggcccatc aaaatgaaaa ctaaaggctg
     7441 tggtatttcc tggtctaaaa gctgaactga ccgtcactag tattgggaat aaaatcacga
     7501 tcactaagga gattagatag agataagtca ataattgggt gaagaaacgt ttgcgtttca
     7561 tggatattga aacataggtc tttctcatga tctctcctcc atatcaaaag ctttaaattt
     7621 cttaaaggta atcaacgata ccgaaatcac tattacagag atgattaagg tcactgccga
     7681 cgccatcgaa aactgaggcg aggtttgtgt ggtcaacttg taaatccaag aaatcagaat
     7741 atcagttgtc ccagctccgc cccccacagt accagggccc ccattattaa acagataaat
     7801 aatagagaaa ttattgaagt taaaggtgta ctgagagata aaggttggtg ctgcaaccgc
     7861 aaaaatcatt ggcatggtga tatggcggaa tttctgtaag actgtcgcac catcaacggt
     7921 tgctgcttcg tataaatcag ctggaattgc ctgtaaaata cctgatacca aaatatagat
     7981 atagggaaat cctagccaac cttgcaccat aataacagcc acttttgtcc agaaggggtc
     8041 tgtcttccag gacacaattc cgaagtcaac gaaaggaagg aatttctcga tgaaaggaag
     8101 aacctgaaca ttcatggccc caatggaatc attaaagaaa ttaccaaaac tcataataga
     8161 gataaatgct ggaacagccc atggtaacaa gaatatcaca ccaaagagac gttttcctct
     8221 gataaaggat tgattagcaa taacagccgt tgcaattcca ataacaattt gaagacttgt
     8281 cgctgctaat gtccagatca gcgtccatga caaaacagat gtaaatgctg ttttgaaggt
     8341 gcttaattct aagatattaa gaaaattctt caaccctacc caatccaata aacgatacgg
     8401 aggaatatgc ctaaaatcgt aattggtaaa agcaatcagc aaggtcacta aaactggaaa
     8461 aataatagcg aaagccattg ctaaataagc cggaatgatg agcaagtaag gaaatccttt
     8521 ttcataaatc gcttcaaggg tctctttagc cgtcaccgga accttcttgc cttcattcat
     8581 ttgatgtgca actaatttag catctctcat agcaaccaaa tgaaaaatac caaataagac
     8641 tagagtaatt aattgcatgg cccccttaat taacataaat aaggagtgat cttgcattgg
     8701 aacacttcca agactgtaaa aacctgttaa agcaggaatt cctagaacta caatttctag
     8761 tagttctagg aagaaaacaa ctaataagac tcccccttta acgatttgtt tattgtatag
     8821 ttgtcccaaa cctgggataa cagatagcca catggcttta ttaggcttct gttgtctttg
     8881 gttatctacc attttttatt cctcttctca taaatgattt aaggttatac tccttatcat
     8941 actaacttcc ttttaagctg actaatttat ttttttatag taatggtttg tgcttcatga
     9001 cctgtcttta tactataagt ctcaggactt gtccataaaa cattcccaat actatctttt
     9061 ttaacaagtt tgactgctat ttctttattg attggtagat gagtatcaaa gaaccagttt
     9121 gggtacttgg caatggtctg agtgttatta aatagaggac caacagcatt cttagcgtca
     9181 tttgccccca tctcaaaaac atcacccatg agatatagtt gttcccctgg tactgtctta
     9241 aaatcattga tgagtaaacg aacaggaatt tgtttatcag tcaatacttc aaaggcttgg
     9301 taactattgc tggtttgctt atcggcagtt gtcactgaaa tgttataata atttgctgga
     9361 accgtcggta cttttaaggt gataagggta tctgaccaag aaaggatctc agcagagatt
     9421 tctccaaaag tcacttgacc tttagaatta ccaaaacctt gacctgaaat agtaatctta
     9481 tttccagcaa tacccactga agcatcgaca tctccaagtt gtggtgtctt gtcctctcct
     9541 tcataagtcc atacagcgac ttgccccgct ccaagttcaa agctactaat agtgccatct
     9601 gctccaacta ttaattcttg accgccaaga agaccttcta atttgtcctt gtagctattt
     9661 tggggcaagg ctgttttagc attagtaatt gtataaccat tcgtttgatc tctattaata
     9721 gccactagtg cgacatgatt accaaattta cgctcaaata ccaaaacatg atcactaatc
     9781 caacgttgtt cagttgttcc ataagctaaa gcttgatttt gttttcttaa aggagctagc
     9841 ttactaatca ctttataggc ttgtgactct ttattaaaac ttggcatatc accacgatta
     9901 ttaggatctt tatcacctgt tgcatactgc tctgtaccat aataaatatt aggcacacct
     9961 ctagatgtta aaagcaaagc ataggcttga ttaacagcag tttgattatt agccactttt
    10021 gttgcgaagc gttccatatc atgattatca ataaaggtca cctggtctgt cacttcatta
    10081 gtcacctgat ctctattttc aagaaccttg taaaaatctc gcatggtaaa cgttgaaaaa
    10141 cctgtataca actgtctaat agcattggca aatctaaaat ctaaaagccc catcccacta
    10201 ttgttagcaa atgtcgtcat atcataatca tcgtcggtat gtcccgagaa ccattccccg
    10261 aaaacaaaga cattatgttt ttcatagata tgactcaacc aatttttttg ccaaccttgt
    10321 gacatatgtt taacagcgtc aactcggata ccatcaacac ccaggtctaa ccatttatca
    10381 atagcttctt tcatgtactg gtcaactttc ggattaatgt tatttaaatc agctagtccg
    10441 tacattgaat gataaataga attttcataa gtactaaaat cggtccaaga ttcatgatta
    10501 aaaatcttgt ctttatcatc tgaaaattta ccaactaatt taccgttttt atataaagcg
    10561 ccatcttctt tgaaagttgt gccttctttt tcggctgtag acgtatgatt aggagcaaaa
    10621 tcaataacta ccttaatatg gtgttgatga gctactttga ctagttgttg aaagtctgct
    10681 tcagtgccaa aatgctggtt tgttttaaag aagtccttag cccaataccc atgatatgca
    10741 gcacttccat tagaaggatc aatactgtcg atattttcaa caggagaaga aatccaaatg
    10801 gcagaaatcc ccatatctgt caggtaacca tccttaatct tggcgatgat tccttgccaa
    10861 tcacctccat ggtatttttt aaggtcattt ttatcaaaaa catcagtctt accattatta
    10921 gaggtattac catcgttaaa acgatctgtt acaatctgat aaatcgtatc tgttgaaaaa
    10981 tctgctttgt tggtgacaga agcattatcg gcagcagaaa cagttagtgg aaatgaaatt
    11041 aagccaagta aaacagcact tttcgttaat aacttataag ttttgatatg taattctctc
    11101 atcgcttttt ccttttcaaa taacctttta gttactaaac acaatatatc catcctggag
    11161 taattgtttt ttgtttttag ttaccccatt gcttgtcaaa atctgtcctc cttccttaat
    11221 agaataagca ctctcaccat tgttaagaac aagtgaaatg ctttgctcac ctaagctgcg
    11281 tttcagaatg aggcagtctt cgtcataatc aatgctaata gctccttctt gaagagctgg
    11341 ctgttggcga agagcaataa tcgcttttac tttgctaaag aaatccttgt cccgctcagc
    11401 atcatcccaa ggaaaaccgc gacgcgaatc agggtcgtaa cccccttcca ttgccacttc
    11461 tgtcccataa tacaagcaag gaatgcccat aaagataaag agcaatgcta aagcagctaa
    11521 caatttatct ttggaaccct taacttgagt gaagaaacga tgcgtatcat gagaatccaa
    11581 aagattcaag ttcatgcgat tgacttgcca agtgtttcgc atcaaaatgt tgcttaattt
    11641 ctcagccaat tgttttgatg tcatggtttc aaaagcaaag taatccaaac aggctttggt
    11701 gaaagcataa ttcattatcc catcatactg atcccctgcc aaataaggat aggcatcatg
    11761 ccagttctcc ccaattaaaa tggcatctgc tttttccgct ttaaccgcct gacggaaacg
    11821 tcgccaaaaa tcatgagata cttcatcaga aacatctagg cgccaaccat cgatacaaaa
    11881 ttctttaatc cagtatcgcc caatctcaat cagatagtct tgcacgtctc tatttgaggt
    11941 attccatttg ggcatatagt tacatccagc aaaggtttca tagtttacta ggtccatgga
    12001 tgggtgttca tcatgagtca taaaccaatc aaaaaactta gactctttac cgtatctcaa
    12061 gacatcctga aattccacgg catcactgct ggcatggtta aagacagcgt ctagaataat
    12121 cttgattccc atttggtgag ctaaatcaat caattcttgc aaatcatatt tagttccaaa
    12181 ttgtggatca atagcgtagt aatcactaat atcatatttg tgattactaa tggattgaaa
    12241 aattggcgtt aaatagatga cagtaatccc taaatctttg aggtaatcta atttttctgt
    12301 aattcccttt aaatctccac cagcaaaact tttaggagtc ggtttttcta accaacccat
    12361 gttgacataa ctgtcacctt tttcccaatc cccacggtta aagcgatcaa caaagatttg
    12421 ataaaaaacc gcgtcttttg tccaaggaat gacttgatga acatcaacag cattgatata
    12481 aggcatttgg aagaaattat aaaacccatt tgaaaaatcg taactagtag ttaacccatc
    12541 ttcactaaaa taataggttt gaccttcttc ttcaatttta aaaatgtagg ccaacctcac
    12601 atcgtctaat gttaaagtag tttcataata agcatgcgta tggtctgtat aggcaactga
    12661 catttccaat tcttcttgga attcatgata accatattta ggcccgtgga ccaaattaac
    12721 agtaatacct ctatcttctt tggccacgcg taatcggatg acaagttcct tttgagatat
    12781 ggcaaagcaa tacctcgaat caggaatatg gagcattccg gcaacattca tttctacctc
    12841 cattaataaa gtattgacga ttacttcttt ttctttaaaa cgaaaaaata gcagagcgac
    12901 tcatacttct atatttttag tatctgtttc atcttcatca aactaacaga aatagaagct
    12961 gggcgtactt ctttatccca gcctcccttc tttgccttat tcagcgtatt tttgttcaat
    13021 agcttcctta atagtttttg ctgcttcttt tgcagcttta gatgcttctt ccttaccaga
    13081 agccacgtta aagaacatat ttgcacctgg ttcccaaact tcagccattt ctggaatatt
    13141 tggcattggt tgtgcgctct caaactgact aatcacagct ttcgttaact cattaccctc
    13201 tttagaagca tagttacgtg ctgttaagtt agctggaatt tcttgtgttt tatcatagaa
    13261 acgtttttgg ttttcagcat tggtcacata atcaaggaat ttttgagcag tcgtttttcc
    13321 tttagaatag tttgaaataa cccaagcttt accacctgcg aacggttggt attttttacc
    13381 atttgtcaaa gtaggaattt ccattacccc aaagttaaca ccagcatctt taaaggagct
    13441 agctgcccat ggaccatcaa taatgacacc agctttttta gaaataaatt gttcagtgat
    13501 aaaatcacca gcttttttag tgtcttgcat tccttgaggc caagtgccat accactgttt
    13561 cgcgtagttt aacccttcaa cagttccgtc gttacctagg cctaaatcac ttggtttggt
    13621 tcctttatca ccaaagatat aaccgccata accggcaatt aaaccataac cataatagaa
    13681 gtctgtccat ttcgccaaga aacctacaga ttttcctggt tcagaggcaa aagcaaactt
    13741 agaatctttt tgtaagactt ctaattcagt aaagctttta ggtgcttgag gcactaaatc
    13801 cttattataa taagttacca aagtttcaat tacgtctggt gcaccataag ttttaccatc
    13861 aatggttact aatttcttaa ccgtatcatc aaactctttg ctgtttccta aagtaacctc
    13921 agagatttga ccttcagttc ctaagccacc tacacggtca aaaggtgcta aaaagacatc
    13981 tggtgaagct cctgttggac catccgttga tagcttatca agagtatcca tcataccttc
    14041 ttttttgact ttaacggtga ctttatgctc tttttcaaac tcacccttaa tactcttaat
    14101 gtaatcaaca tatccctcat caacagagac tacaatagtc tttccgtctg tttccttaac
    14161 gcctttacca ccaccactta cgcaagcagt taggggtagc actgaagcta aggataaagt
    14221 tccaagcaca gatgcttttt tccaattcca tgacataaca tgttcctccc gaaatggttt
    14281 tttcttaaac acaaatcaaa tcatctatct actctcatca tattagcaaa taaaagataa
    14341 cataaggcat ttaaaattaa taacagtaat agtttcgaaa cttaaacgtt taagttaagt
    14401 ttatcgtaat cgctttcatt tgtcaataac ttttttactt ttcttttaag agcatatcag
    14461 tagtttttct gctaattgaa aacaaggcat ttactgtcac acaagtctgt cctttctttt
    14521 ttctacaact ttatttcgtc agcgtaaata aaacaaaagc tctataatct cttgaatggg
    14581 taaacccact gtagagatta tagagctttt tgaatatcaa aaaagtccca taaaatttat
    14641 aatgaaaagc aaccaaactc tcattagaaa gactcttatg gaacatatca atcatacctt
    14701 acaacacatc ggaattaaag acaaaaatat cactctaaac aatgttttga aggatgaatc
    14761 tcctactgag ctcgttacta cttaccagaa aggtagatag gaaatcctga tgaagagtgt
    14821 gtccaaggga ttatcaatta ggtcttagat tacaaaatag taattcaggt aggctctact
    14881 gttttcaaca ctagttctgc aatttgtttt cgagcttgct tagcttctct tattggaaaa
    14941 ataacatacg catgaatcat tttttctgca ataatcagct gattagcaat tcgttgtctt
    15001 gttaattgat catgtaaaga cataatgtct gggtaaaaaa tttcttttgt cccaacaaag
    15061 gtcgttattt caggcaaatc ctcacaatca ccatacaaag gactgacata aggaagttgc
    15121 atattacctt gaccacctgc ccatagttca cctatatcac gaagacgacg agcatctagg
    15181 taagcttctt ctgcttcaaa atccttgtag gcttgatttt taaaactaac atctaaccaa
    15241 ggagatataa gaattaattg ctttggctgt agtgctgttt tagtatctcg tatatactta
    15301 gcaaacgcca aagccatccc cccaccagaa gaatcaccaa tgacactaac ttgactcggt
    15361 ccattagaca agcaaaattg ctggtaagct tcaactaatt taggatagac atcttgataa
    15421 gtagcatacg gtactttagg aaagttagga aaaacaacct tggcattagt cagctgtata
    15481 agatgcttaa ccgtcttaaa atgaagtttt tcgggtcgtc gtaaatagac actgccataa
    15541 agataataaa taacaggctg atgactttgc ttttgatcat taataataaa aacttgtatc
    15601 ccgttcacat cgtattcttg agttgaaaaa cctactatct cttcaggatt atctagatga
    15661 taagggatat tacgattacg ctgcgtctca acccccttat aattatcaac ttttttagat
    15721 aacttatact ttctataagc aaacattttc tcaaccaacc aacttgaaaa ggagcgcttt
    15781 tgaccatatt caactatcaa aatacctagt atcactccaa aaaaaatcac cacagcaata
    15841 atgaccactt attatcctcg ttttctaata ttattttact attgcaagtc tttcctattt
    15901 gcccaaattt tactataaaa ataagggttc attttatagt tgggcactgg ctgcttcgtt
    15961 tctagaaatg gttgcacttt cttatcaaaa gctaaccagc cattccaacc gagatggatg
    16021 gtatcttgca taaagtagga ctcaccacca tcttttgaga agtcagcaat gcgatgaaat
    16081 ccttgtgact ttaactggaa ttttatttta cgaactgccg cttgatactt atcttgattt
    16141 aagccggtat aatccgccca agctttatta acaggagtta taacaaagag tacatctgtt
    16201 tttcgtttag caaattctga taataaaagc tgaaaatcat tgtattctgg tgacgccagg
    16261 taactataat taacttggaa attcttataa agattgtatt taggtgctat tcgtttacga
    16321 taaaatgtat ttttaatccc aaaacggttg ttggttgttg ctaattggcc tctcttagtt
    16381 gctaacgcat tcaattgttt atacgaaaat actttaggta atcccttaac gcgaggcaaa
    16441 attctttttt catagttagt agatttgcct aaaaaactaa aaagggactc ttctctcaat
    16501 gctacttgat gttgacattt caaaatagct ctgtctaacc gactaagaga cttaccctta
    16561 cttacttttt tcaataagtt tgatttagac acaccagggt taagctcaag caaacgcttt
    16621 gctgcaaact gtgattcttt atcagttcta gcttttagta aaaattcaat cacttgagtg
    16681 ttagacaagt acatctgaac cgcactagga ttaatccctt gagcagtaaa ccattgagga
    16741 gatactacaa agatggcttt tttcttttgc atttcattgg taatttgttg cataccataa
    16801 taatgcgaca aagatgctga tcctctctta ccaattaaaa atggtctata gctccgcttg
    16861 tagcgctctg caagcaccga agggtgcata ctatccattc gactccattc gctagaacca
    16921 aaaaaaggca caaaacgaca agtctcatct gataaagctt gtcttttaat caaaccattt
    16981 ttaaaagaac tatctgtgat cgcaacggca tttgcttttt cctgagctat ggaatgatca
    17041 agttgtgtag gaaaactaaa aatagtaatc actactaaaa caaaggcaat aagaagagga
    17101 cctagaatta accagagtct cttaagcatg ttgcaattcc tctacacctt gaacaatctt
    17161 agttactgtg ttccaatcat cacgaccaaa ttctgaaata ggtactttaa tattgaacgt
    17221 tgactctaac tcaacaatca attcgactgt cccaagacta tccaacacac ctgcatcaaa
    17281 caagtcttcg tccatcatct ctgaaacatc ttccataaac aaacggtcaa acaattcaat
    17341 tacagtttct tcaatagaca tttttatact tccttttatt aatgtagtgg tctgttaaac
    17401 caaaggtcat ttaaaaaacc agagaataac agaagtgaaa acatgacaac gtggaaagtt
    17461 accacgatac ataagacatg gaatgccctg ctttgaaaga ggggtgataa gccttttttc
    17521 ttacggtgac ggttgatttc ttttttctta cgaatccaag catcattaat aataagtcct
    17581 ataccatgga aaagcccata agcgatataa taccaggtta agccatgcca aaatcccatg
    17641 actagcatat taaccagata ggcaacacct gaagtgacat ttcggttttt aaaggtttta
    17701 tgcttaatca atagatgaac caatcgcata aacacataat ctctaaacca gaaggacaaa
    17761 ctcatgtgcc atctattcca aaaatctttc aagcttgcag ataagaaagg catgttaaag
    17821 ttttctggtg ttcgaatacc caataggtag gaaataccaa tcgcaaacat cgaataacca
    17881 gcaaagtcaa aaaagagatt caagccatac acgtacataa ccagaatagt ttccttatta
    17941 aagtagccac caaccatcaa agccttattt tcaaggagag gaagtaagat gccacccaaa
    18001 caataggaaa tgatatgctt gtataggaaa cctaacatga gatacatgac tgccttattg
    18061 agcattgcaa aataatcatc acgttcagga agtttatgat aatcttcctg aaaacgtctg
    18121 aagcgatcaa tggggccact tgagaaagtg gggaagaaaa tcatgaagcg aataaaatcc
    18181 ggtaaacgaa cctcttggag agtcccgtca cgcatctcaa taatcattcc aatactttta
    18241 aaggtcaaat atgaaatccc tagaaaacta aagagtgttt gagaagaagg acctgttagg
    18301 ggagcaactt tgacccagat aagggggaat aaggccatcg caatagctaa gtaaaacact
    18361 cctgcactat tagctttctc acggtatttc tggtaagcaa aaacactgat gatttgccac
    18421 aaaagataaa agagaaaagc aactaattgt tgataatgat ctcctccaaa cataaagaga
    18481 ataaatacca aacttaccaa ggtttcataa gtgcttaagc gcttttgttt aaaaatccca
    18541 atgatgactg gtaagaaagc caaaataaga tagacaaagt aaatggggtt accgtaaggt
    18601 tccatgtagg gaatgtggct aaaaaacatc atcatcggtt attgacctca ttaatcaaag
    18661 ttttaatatc tatcttacca tttggagtta atggtagact gtctcgataa agaaacttag
    18721 aaggcatcat ataactcatc atatgatcct taacagaggc tttgatagct ttggttaact
    18781 ccaattccct atcaaaacgc tctttaaccc catctttgac aacgatatag gccaagagat
    18841 tttgaacctt atgttcttta ttgtaccttg gaacagccac agccgatgct accattggtg
    18901 attgattcaa ctgttgggaa acatcttcta gttcaatacg ataaccagca tatttaatct
    18961 gaaaatccaa acgcccacca tacagcaaaa tattatcttc tgtcagtgag ccaatgtccc
    19021 ctgtatgata agcaggctgt cctttaaaag taaagaaagc ttctgcggtt ttctcaggat
    19081 tgttgaggta accttttgac acagcaggcc ctgtaacaat aatttcaccc tgctcccctg
    19141 aagctaactc cttaccatct tcatcaataa tataggtcgg agaatctggt ttggggtagc
    19201 caatcggcaa gcgggtataa ttatccacca tttctctggt aatttcaata gcggataagg
    19261 ctacagtcgc ttctgttggt ccataggcat tgatgatctt ggcactcggg aaacgttcaa
    19321 agagttttcg agccgtagaa acggttaatt cttcgccatc aaaataaaaa tgcgttaaag
    19381 ctggcatctt agcctggcaa aaatcatcgc taagcatggc catatcagca aatgaaggag
    19441 tcgatgtcca aatgccaact ggaagttgcg caatcgttgt aaataactgt ttaaagtctg
    19501 ccaccaattc ttttggaagt gcaaataagg ttccacctaa agccaaggta ggtgcccagt
    19561 acatgacaga aaggtcaaag gaatagggtg gctgcgccag catttgtggc tgtttaggta
    19621 catcaaatgc tgcatcttcg atcatccagt tggtaaagct caaaagattg tcatgtgaaa
    19681 tttgcacccc ttttggttgg cccgttgttc cagaggtgaa aataatgtaa taattatcat
    19741 ctcctttaac agaatgtgtt cgctcatagg gcatttctgc taattttgct gactcaatct
    19801 ctgatagtga gacaagggaa atcccttcaa tagtaagagg gaattcctca atagcaatga
    19861 ttaaactggg ctttgcaatc tcaataatcg ctaaaatacg ttctggtgct gaatgcacat
    19921 ctactgggat ataggcatga ccagatttgg ttaaagctac aaaagtagct aacatatcat
    19981 aagtttgcgc cccaaaaacc aaaactggag attttgccag taaagctaag ctatctataa
    20041 atgcagcaat gctatcagaa tctcttttga gttgtccgta agtgcggtgt tcccctaaac
    20101 aatcataaac tgggaaatca gcctgtgtct gagcaaactg ctcaatactg tcaatcatat
    20161 ccttaatcat cagtcaatct cctaaaattc attataaata aagttacttt gaccatgtcc
    20221 tagataacca aagaaataga ccagcaacat taaaatcaga taaaataaaa ttgtcttgcc
    20281 aataaaaaca gcaattcctc tcattcttga tttgtttttt atc
//
LOCUS       DATUYR010000029        20158 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.029, whole genome shotgun sequence.
ACCESSION   DATUYR010000029 DATUYR010000000
VERSION     DATUYR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20158
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.029"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            281..715
                     /locus_tag="VCI33_001014"
     CDS             281..715
                     /locus_tag="VCI33_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831947.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIATE
                     TVEESLGQVKEKVEGIGEATSQTIQSKMEKLTSDKTDADDEK"
     gene            complement(765..1676)
                     /locus_tag="VCI33_001015"
     CDS             complement(765..1676)
                     /locus_tag="VCI33_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP2831948.1"
                     /translation="MKTVRIFYNPNSGKKENQLARQVKDYFCQHGFSEDSVEVITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIMQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGEFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(1793..2443)
                     /locus_tag="VCI33_001016"
     CDS             complement(1793..2443)
                     /locus_tag="VCI33_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEP2831949.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(2440..2880)
                     /locus_tag="VCI33_001017"
     CDS             complement(2440..2880)
                     /locus_tag="VCI33_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEP2831950.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLLQEVIQ"
     gene            3099..3947
                     /gene="ylqF"
                     /locus_tag="VCI33_001018"
     CDS             3099..3947
                     /gene="ylqF"
                     /locus_tag="VCI33_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEP2831951.1"
                     /translation="MATIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTHY
                     PSRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDVD"
     gene            3937..4728
                     /locus_tag="VCI33_001019"
     CDS             3937..4728
                     /locus_tag="VCI33_001019"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEP2831952.1"
                     /translation="MSTSIKAIKESLEAVTSLSDPLFQELATDARLGVQKALKSRQKA
                     IQADLAEEERLEAMLSYEKALYKEGYQAIAGIDEVGRGPLAGPVVAACVILPQHCKIK
                     GLNDSKKIPKSKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            4793..5629
                     /gene="dprA"
                     /locus_tag="VCI33_001020"
                     /pseudo
     CDS             4793..5629
                     /gene="dprA"
                     /locus_tag="VCI33_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
     gene            5736..7865
                     /gene="topA"
                     /locus_tag="VCI33_001021"
     CDS             5736..7865
                     /gene="topA"
                     /locus_tag="VCI33_001021"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEP2831953.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGSLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(7940..8824)
                     /locus_tag="VCI33_001022"
                     /pseudo
     CDS             complement(7940..8824)
                     /locus_tag="VCI33_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8837..9382
                     /locus_tag="VCI33_001023"
                     /pseudo
     CDS             8837..9382
                     /locus_tag="VCI33_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            9397..10389
                     /locus_tag="VCI33_001024"
     CDS             9397..10389
                     /locus_tag="VCI33_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEP2831954.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESPLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLREMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADFVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARGALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            10494..11159
                     /locus_tag="VCI33_001025"
     CDS             10494..11159
                     /locus_tag="VCI33_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEP2831955.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLAFRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            11152..11868
                     /locus_tag="VCI33_001026"
     CDS             11152..11868
                     /locus_tag="VCI33_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEP2831956.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGVCMFMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            12014..13360
                     /gene="trmFO"
                     /locus_tag="VCI33_001027"
     CDS             12014..13360
                     /gene="trmFO"
                     /locus_tag="VCI33_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEP2831957.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAKSVTAELENHPLIEVIRDEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHDALTNAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRNNPNLFFAGQMTGVEGYVESAASGLVAGTNAARLFKREEALVFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            13513..14919
                     /locus_tag="VCI33_001028"
     CDS             13513..14919
                     /locus_tag="VCI33_001028"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEP2831958.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQVRQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            14962..15276
                     /locus_tag="VCI33_001029"
     CDS             14962..15276
                     /locus_tag="VCI33_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831959.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            15276..15626
                     /locus_tag="VCI33_001030"
     CDS             15276..15626
                     /locus_tag="VCI33_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEP2831960.1"
                     /translation="MKRYEVTVNGQVYEVSLLELADGETVQVSRPAAPATEKEMNANA
                     AGGGLQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            15636..16766
                     /locus_tag="VCI33_001031"
     CDS             15636..16766
                     /locus_tag="VCI33_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEP2831961.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKVMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKVGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(16967..17851)
                     /gene="citG"
                     /locus_tag="VCI33_001032"
     CDS             complement(16967..17851)
                     /gene="citG"
                     /locus_tag="VCI33_001032"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEP2831962.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGCFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(17844..18539)
                     /locus_tag="VCI33_001033"
     CDS             complement(17844..18539)
                     /locus_tag="VCI33_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2831963.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKTTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(18697..20100)
                     /locus_tag="VCI33_001034"
     CDS             complement(18697..20100)
                     /locus_tag="VCI33_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEP2831964.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
BASE COUNT         6383 a         3747 c         4073 g         5955 t
ORIGIN      
        1 agtgatgtgg tcactttgac aaccatttat tggtcctttt agataagaca attttgtggc
       61 tcttcagttg aaaggatgtg ttgttgatgt tagaggaaac aatcatgtta aattaactca
      121 ggcttaaaga ccaaaaaagg agtgaagtct attatgaaaa aaagaaattt taaacttaca
      181 gaaatgttac aattggtttt attagtggtt cctgctgtaa aacttgctaa aaaaatgttt
      241 aacgatagta aaacaccgtc taaaagaaag taggtctaca atgactaaaa aaaatcatgg
      301 cgttttttgg gggttgttgc ttggatcagc agcagctagc attgcttact taagtttatc
      361 gtcttccaaa aaagatcaac tcttgaaaga tagtgctaaa aaaatagatg atctcaacgc
      421 ttacttgcaa gataaaagta agcaagttct ggatgcagta tcggaaaaag tacaagaatc
      481 caaagatgct gttgaagttt atggtggtat tgccgcagaa accgttgaag aaaagtggca
      541 agataccaaa gatgctgttg aagtttacgg tggtattgcc acagaaacag ttgaagaatc
      601 tttgggacag gttaaagaaa aggtagaagg aattggcgaa gctactagcc aaactatcca
      661 atctaaaatg gaaaaactca ccagcgataa gacagatgct gatgatgaaa aataggtttt
      721 aaaatacctt taagataaga caaaaagtgc cattaggcac ttttttaatc ttctgttgga
      781 acaataatcg aaatggcttt tggaatgata tctaattgaa tgggcagata gtcactttta
      841 tccccatcaa tcctggtacg tggtaatatc gcttgttttt tagcttgtgg tagtaagcta
      901 agcgtattgg cttgaaaatg actgatttcc tctgctttgg aaaactcacc ctgtttaaaa
      961 tcattaatat gccataaaaa tttaaagaaa gagacttttt tcatggtata aacttggaaa
     1021 tagccatcgt cagcttgagc accaggagaa aaagaaggaa aaccagcaat ggtatttgtc
     1081 atcgtgatta agagaaactt cgttttcagg tgaatacgat ggttgtggct aattaaagag
     1141 atggcatagc tgcgatttcg tttcagtatt cgaatactat cttttaaaaa tgctagagga
     1201 ccaaagcgtc gtttttcctc agccgttacg tcggcagcaa tatcagccag taatcctaag
     1261 gttaaactgg aaatcatgta ttgctggtta gctttgcaaa tatcaacttg catgatttgt
     1321 ccagtcaaga tagtatccaa cgcctcagta atttgtaaag ggatatgcat agccttggca
     1381 aaattattga ctgtccctga gggcactaag cctattaaac aatgagctcc tccttcatag
     1441 atgccaccga tgattttatt tagtgtccca tcgccaccaa gagggataac caagtcaatc
     1501 ttgtccttag cagcttgttt tgctaattga aaagcctggt cagcatcttt aggggtaata
     1561 acttctacag aatcttcgga aaaaccatgt tggcaaaaat agtctttgac ttgcctagca
     1621 agttggtttt ctttctttcc agaattagga ttgtaaaaaa tccgaacggt tttcatcagg
     1681 tctctccttt gacagcttat tacttgctat ccattatacc acttctaaga attgatgtct
     1741 tacatcaaat catcacatta agcctctcaa tcagtctgag catctatttg tcttaaagca
     1801 taaagaaagt gatagcgata aattggaggg cagaggccaa taaaataaag agatgccaaa
     1861 tcatgtggaa gtagggtctt tttttggcat aaaataccgc tccaatggta taagacaagc
     1921 caccaaataa catgagaaga ccaaaagcaa gactagtttt ttgaatgatg actggcaaga
     1981 taaacacaac gagccagccc ataacgatat aaagcatcaa actgaatttt tcattgatac
     2041 gtttggcaaa aattttgtat aaaatcccaa aaagcgtaat gccccactgc aaaacaataa
     2101 tgatatagcc tagccaacca gaaacaaggg ataaggcaac aggtgtataa cttcctgcga
     2161 tagcaatgta aatcatactg tgatcaataa tgcgtagaat gtatttgtga accgaaccat
     2221 aagccatgga gtggtaaatc gtagaggaca aaaacatcaa aaagagactg atgacaaaga
     2281 tggagatacc aatagcggct ttaaggtcat aagtttgata agcatagctg gctgaaatag
     2341 gaagtagaat gagcatagca aatgctccga tagcgtgtgt cacgctgttt gctacttcct
     2401 cactaaaaga taagggtaaa ctttgtttaa aggtttggtt cattggatca cctcctgtaa
     2461 taaataaata gttttgtggt aatctttgac cgtttgacag gcaacctgca caatcggtgg
     2521 aatgtcctcc tcaatgggag ccttctgatt ccaacaggtt acaaaagtat tgtaaagact
     2581 ttcagaatca gcctgtgcct gaagttcctc caatacacga ctaatatctt gaataggaaa
     2641 taccgtttta aacaaactga tggcaattaa gcgcgctagt tgttgctttt ggtatttttt
     2701 cttgataggt ttagtcacat acccatgctt gacgtaatta ttaatcatag atgccgttag
     2761 tgatttatta tctgacactt ctgaaaaatc agtgcactgg ttgacataaa gtaagacttg
     2821 atccaaatat aagtctaaat cagggaggtc cttccaataa ggtaatgatg gaaaaatcat
     2881 agttttgtct ctctatctag ttttgataac tagattatac gattaatgaa aacttattgc
     2941 aagcaaaaac tgtaaagact taaaagagga aagtagaaat ggcgaagtga agctaatcat
     3001 gctataatgt taaagaattg attgggatag atgccttctg tcgcaatcag actattttat
     3061 cacgaagaag atgatgctac gaaaggaatt taaactctat ggctactatt caatggtttc
     3121 ctgggcacat gtccaaagcc agaagacagg ttcaggaaaa tgtaaagcat gttgactttg
     3181 taactatttt agtggatgca cgcttaccac tttctagtca aaatcccatg cttacaaaaa
     3241 ttgttggtga taaacctaaa ttaatgattt taaataaagc tgacttggca gatgcaactc
     3301 gaactaaaga gtggaaggct tattatgaaa gtcaaggtat taaaacatta gccatcaatt
     3361 ccaaagaaca atcaacggtt aaaaaagtaa cagaagcagc caaggaactc atggctgata
     3421 aaattcaacg cttgcgagag cggggcattc aaaaagaaac cttgcgaacc atgattattg
     3481 gcattccaaa tgctggtaaa tctaccttaa tgaatcgctt ggctggtaag aaaattgctg
     3541 tagttggcaa taaacctggt gtgactaaag gacaacaatg gttgaagtcc aataaggaat
     3601 tggaaattct agacactcct ggtattttat ggccaaaatt tgaggacgaa ttggtgggct
     3661 taaaattagc tttaacaggt gctatcaagg accaattact accaatggat gaggtcacta
     3721 tttttggttt gaattacttt aaaacacatt atcctagtcg ccttactaag cgttttaaga
     3781 atattcctct tgaggaagaa gctcctgaaa tcattatgac cttgacacgc caactgggat
     3841 ttaaagatga ttacgaccgt ttctacactt tattcgtcaa ggaagttcgt gatggtaaat
     3901 taggtcaata caccttagat caggtgggag atatggatgt cgactagtat taaagctatt
     3961 aaagaaagcc tggaagctgt tactagcctc tcagaccccc tctttcaaga attggcaact
     4021 gacgctaggt taggtgtcca aaaagcgcta aaaagtcgac aaaaggctat tcaggccgat
     4081 ttagcagagg aagaacgatt agaagccatg ctttcttatg aaaaagcgct ttataaagaa
     4141 ggctaccaag ccattgcagg tattgatgag gtgggacgtg gccccctagc aggtcccgtt
     4201 gtcgcagcat gtgtcatctt acctcaacat tgcaaaatta aaggtctgaa tgattctaaa
     4261 aaaatcccta aatctaagca tgagaccatt tatcaggcag tgaaagaaaa ggctttggct
     4321 atcggtatcg gtattattga caatcagctt attgatgagg tcaatattta tgaagcaacc
     4381 aaactagcta tgctagaagc cattaaacag ttggagggcc aactcacaca accagattat
     4441 ctcttgattg atgccatgac attggatatt gctatttcgc agcagtctat tcttaaaggc
     4501 gatgccaatt ccttgtctat tgcagcagca tcaattgtag ctaaggtcac cagagatcag
     4561 atgatggcta actatgatcg catttttcct ggttatggct tcgctaaaaa tgcaggctat
     4621 ggcaccaaag aacatttaca gggattaaaa gcttacggca taacgcctat ccatcgtaaa
     4681 agttttgaac ctgttaaatc catgtgctgc gattcaacta atccttaaaa agttctgttt
     4741 ttagcagaat ttttttcttt tttacgaata aatgaagtaa ggaggtaact tagtgaatca
     4801 ttttgaactt tataagctaa agaaagctgg actgactaat aaaaacattc tcaatattct
     4861 tgactaccaa gaacaccagg aaaaatcgtt gtcacttcga gatatggccg ttgtttctgg
     4921 ttgtaagcat ccgtctcact ttatagaagc ctataagcag ttagatattc aaaaattaaa
     4981 aatagaattt aaacaatttc ctagtatttc tatcttagat aagcattacc caatggcatt
     5041 gaaagaaata tacaatccac ctgtcctctt gttttttcag ggaaatttag accttcttga
     5101 gaaacctaaa ttagccattg tcggctccag acgctcaagc gataccggag taaagtctgt
     5161 ccgtaaaatt cttaaagaac tcgggaaccg ttttgtgatt gttagcggac ttgctcgagg
     5221 tatcgacact agtgctcatt tagcctgcct taaaaatggg ggacaaacta tcgctattat
     5281 tggaacagga ttggatcgct tttaccctaa agaaaatagg gagttgcaaa ctttcttagg
     5341 gaaaaatcat cttgtgctaa cagaatacgg tccaggagaa gaagctttat cttatcactt
     5401 tccagaacgg aatcgtatta tcgcaggcct tagccgaggt attcttgtcg ttgaagcaaa
     5461 aaatcgttca ggttccttga ttacttgtca aattgggata gaagaaggct gagacatttt
     5521 tgctgtccca ggaaacattt tggacgggaa atccgaaggt tgccttcagt taattaaaga
     5581 gggagcaaca tgcgtcacat cgggcatgga tatcctttca gagtactaaa aaagttaaag
     5641 tttcttccta tatagcattt ttttattgac agtttcttaa aaatgggata ttatgctata
     5701 agctttaaac aaactgttat aggaagtgtg tcgaattggg aacaaaaact acaactaagc
     5761 caaaaacggg tactaaaaag tccaccacta aaaagaaatc aacagcgaaa aaaaatctcg
     5821 ttattgttga atcacctgcc aaagctaaga ctattgaaaa atatcttgga cgcagttata
     5881 aagttgttgc ttcggtagga catattcgtg acttaaaaaa atcgtctatg tctattgatt
     5941 ttgataataa ttatgagcca caatacatta atatccgtgg taagggatcc cttatcaatt
     6001 ctttaaaaaa agaagccaaa gctgctaaaa aagtttattt agctagtgac ccggatcgtg
     6061 agggagaggc tatttcttgg catttgtcac atatcttagg acttgatcct caagataata
     6121 accgagtggt ttttaatgaa attacgaaag atgcagttaa acatgccttt gtggagcctc
     6181 gtcagattga tatggacttg gtagattctc aacaagcacg tcgtgttttg gaccgtattg
     6241 tgggatattc catttcacca attctttgga aaaaggtcaa aaaaggactt tcagcaggac
     6301 gtgtccagtc ggttgcttta aaactcatca ttgaccgtga aaatgacatc aaagcatttg
     6361 ttccaaagga atactggtct attgatggtc tttttaaaaa aggaactaaa aagtttcaag
     6421 cgacttttta cggtattaat ggcaaaaaaa caaagcttga taataacaac gatgtcaaag
     6481 aggtattagc aaagctaaca aatgaagatt ttcttgtcag caaggttgac aagaaagagc
     6541 gacgtcgtaa tgctccctta ccttacacaa catcatctct tcaacaagat gctgctaata
     6601 aaatcaattt cagaacgcgt aaaaccatga tggtagccca acaactgtat gaggggattc
     6661 atttaggaga aaatggaact caaggtttaa ttacctatat gcgtacggat tctactcgta
     6721 tcagtccagt agcacaaaac gatgctgctc aatttattat caatcgcttt ggagctaatt
     6781 attcaaaaca tggtaatcgt gtcaaaaata ccagtggtgc tcaagatgct cacgaggcga
     6841 ttcgtccatc tagtgttaat catacgccag actctattgc caaatacctt aacaaggatc
     6901 agttaaaact ttatacgctt atttggaatc gttttgtggc tagccaaatg acagcagctg
     6961 tttttgatac cgtaaaggtg aatttggagc aaaatggcgt gatttttgtt gccaatggta
     7021 gccaaatgaa gtttgatggt tacatggctg tttacaatga ttccgataaa aacaaaatgt
     7081 tacctgagat ggcagaagga gaaacggtta aaaaaatatc gacctctcca gaacagcact
     7141 ttacgcagcc accagctcgt tattctgaag caactctgat taaaacgcta gaagaaaatg
     7201 gtgttggacg tccatcaact tatgccccaa ccttagaagt gattcaacgc aggtactatg
     7261 ttaaattatc tgctaagcgc tttgagccaa ctgagcttgg ggaaattgtc aacaagctta
     7321 ttgtcgaatt tttcccagat attgttgatg ttgcctttac tgccgagatg gaaggtaagc
     7381 tagatcaagt tgaaatcgga gaagaacaat ggcaacatgt catcgatcaa ttctatcaac
     7441 cctttgtcaa agaattgaat aaggctgagt ctgaaattga aaagattcaa atcaaagatg
     7501 agccagctgg ttttgactgt gatgtttgtg gccacccaat ggtcattaag ttaggacgtt
     7561 ttgggaaatt ttacgcgtgc agcaacttcc ctgagtgtcg taatacaaaa gccatcacta
     7621 aagaaatcgg tgttacttgt ccggtttgtc ataaaggaca agttatcgag cgtaaaacca
     7681 aaaaaaatag gatattttat ggttgtgatc agtatccaga ttgtgagttt atctcgtggg
     7741 atttacctat tggacgtgct tgccctaagt caggtgacta tcttattgag aaaaaagttc
     7801 gtggtggcaa acaagtaatg tgtagcaatg agacctgtga ttacaaggaa gaaaaaatta
     7861 aataaaaaaa gaattggaac tccttgtctt gttccaattc tttttttatt tacaaaggta
     7921 tttttaacta caaaaatcgc tagttcgttt ttaaaaggct taaaaattcc tcaataactg
     7981 gactgaaact cgcatcatct cggtaagcaa tcccaatagg gtaaaaatta tttttatctt
     8041 gcaaaggaat ccaaactaag ttatctacct gattaagact ctcaaaatca ctcgggagga
     8101 tagtcaaggt atttaaatcg tgcaaactat gaataagaac ttcccaatca ttgtgtttaa
     8161 agacaatatc tggttcataa ccgagagctc ggcattttct aggaagcatt tcgcctaaca
     8221 tgtagtgatc gtttaaagaa gcaatcttat agccttttaa atctttaagt tttaactgag
     8281 gatgttgcgc caaagggtgc tgtttgagga gaacgatact gaccttatag ccttttgtgg
     8341 acgtttgtaa tagctcaatg gtaatatcct tttgaatact tagaaaggag aggagaccaa
     8401 tatcgatttt tccttgggac aataattctt ataatttgcg agaaccatcc tgtataagcg
     8461 ataattcaac atttgagtga gtagccataa agattgaaat ttgtttcatg aattgtatag
     8521 caaacagtga tattaattca acacgaatca tctctttttt tcttgattta attgctgaat
     8581 atccgcaaca atttgattaa agtcatccac aaggtctttg cctttgtgat aaagaatatc
     8641 tccagcttcg gttagtagta gctgtcaatc actttgtgta aaaagcgtcg tgttcaaact
     8701 aggactctag ccttttaatt gattgagaaa gagtatgttg cgttacaaac aaacttttag
     8761 cagcgtggga atgatttttt gtttcagcaa ctgcaataaa ataggttaat tatctaatat
     8821 ccatagtaga tccttcatga ttggtcaggg aattgcgtct ctacttggtt tacaacctat
     8881 tttgatgtct ctccttattg ccatgatttt ctgcttcctt attgtttctc caattacaac
     8941 ggtaggaatt gctcttgcga ttaacttatc tggtattggt tcaggtgccg caagctttgg
     9001 actttgctta gcaggttggg ctgttaattc aaaaggaact tcacttgctc acgtcttgag
     9061 atcaccgaaa atttcaatgg ccaacgtttt atcaaaacca aaaatcatgt tgccaatgtt
     9121 atgttctgca gctgtacttg gtgttattgg tgctatcttt aatattcaag gaacacctgc
     9181 tagtgcaggc tttggaatta gtggtttgat cggacctatc aatgccctta atttagctaa
     9241 aggcggatgg tctccagtaa atattctact tatcattatc atttttgtag gggcacccat
     9301 tgtgcttaat atgatcttta actacctctt tattaaggtg cttaaagtga ttgatccaat
     9361 ggattacaag cttaatatct aagaaaggaa cttttgatga aattaaaatt atataatgtt
     9421 agaggcgaag aagcagtctt agcaaaaaaa tgggctgatg acaatgggat tgagatttcc
     9481 ttgacagagt ctcccttaac ccctgaaacg gttaaagaag ctgaaggctt tgacgggatt
     9541 gccaatgccc aaattggtcc tttagatgat gcaatctatc ctctcttaag ggaaatgggg
     9601 attaaacaaa tcgctcagcg cagtgctggt gtcgatatgt ataaccttga tttggccacc
     9661 gaaaatgaca ttattatcac aaatgttcct agctattctc cagaatccat tgcagaattt
     9721 acggttacta ttgcccttaa tttaattcgt catgtggagt tgattcgtga aaatgttaaa
     9781 aaacaaaatt tcacttgggg actccctatc cgcggtcgtg ttttaggtga tatgacagtt
     9841 gcaattatcg gaactggacg tattggtcta gctactgcta aaatctttaa aggttttggc
     9901 tgcaaggtcg ttggatatga tatttaccaa agcgatgctg ccaaagctgt cttggactat
     9961 aaggaatctg tagaagaagc gattaaagat gctgattttg tttccttaca catgccacca
    10021 actgcagaga acacacacct tttcaatgct gatttattta aatcattcaa aaaaggggct
    10081 attttgatga atatggcgcg tggtgccctt attgagacgc aagatttact ggatgctttg
    10141 gatgcaggct tattgagcgg agctggtatt gacacttacg aatttgaagg accttatatt
    10201 cctaagaatt ttgaaggtca agaaattaca gattcactat ttaaagcttt gattaaccat
    10261 cctaaggtta tttatacgcc tcatgcagct tattatactg atgaagccgt taaaaaccta
    10321 gttgaaggtg cccttaatgc tacagtagag attatcaaaa ctgggacaac gacaactcgt
    10381 gttaactaac aactgatttg acgtccatta taaaggaata attccaaaaa ttcctttata
    10441 atggactttt ttgcattagt ccgttataat agactgtaag aaagggggct cttatgacat
    10501 atatagcgct tattggagat attatccagt ccaaacaatt gacggatcgt tccaaagttc
    10561 aaaaaacttt agcagcttat ttagacgacc tcaacaaaac ctttgctcct tatattattt
    10621 ctaaattatc cctaacgttg ggagatgaat ttcaaggact ttttcaggtg gatactccta
    10681 ttttccatct gattgatttg attaatcatc acatggatat ccctattcga tttggagtag
    10741 gagtaggttc tattctaaca gacatcaatc ctgatattag tattggggct gatggacctg
    10801 cttattggca tgctcgtgag gctattcgtt atattcatca aaaaaatgat tatggtaata
    10861 ctacattagc ttttcggact ggtcatcaca accaggatga tgtattaaac agtttactag
    10921 cagctggaga tgctattaaa gccaactggc gagctagtca atgggaaatt tttgacacgc
    10981 ttttagattt gggcatttat gaggaatact ttgatcaaca acgactagga aagcaattat
    11041 ctcttagctc tagcgctttg tcaaaacgtc taaaaagtag tcatgttaaa atttatctga
    11101 gaacacggca gagtgcgcta aactgtctca aacaaatcaa ggaggaaact gatgattaat
    11161 ggtgtctctc actacctagc tcaaacccct acacttaccc tgtttctcat ttgtcatgtg
    11221 ttatcagact accagttaca aagtcagcaa gtagctgatt taaaagaaaa acatttgact
    11281 tatttaggtt accacttgat tggagttagc attcccctaa tttgtttaac cctcattatc
    11341 ccacaagcat ggttgatgag cctattggtt atgataagtc acgcgcttat tgactggcta
    11401 aagccaaaga tggctaactc tctaaaatgg aaaagagagt ggatttttct gctggatcaa
    11461 tgcttacata tcgctattag ttcatttgct gggctaaggt tagcaggggt gaccttgcct
    11521 aattggcttc cgatatctat tttgatgacc gttttattta tcctactcat taccaaacca
    11581 actaatattg tctttaaact ctttttcagc aaataccagc ctgaccaagg agaaaaaatg
    11641 gacacgatca taggagcggg ggctaccata ggaattctag agagaattgt tattggggtg
    11701 tgtatgttca tggggcaatt tgcttcaata ggtctggtct ttaccgctaa atctattgct
    11761 cgctataata aaatttcgga aagtccagct tttgcagaat actacctaat tggttcttta
    11821 tttagtattc tcagtgtttt tatcgcagca tggatttgtt tcttctagtt gaaaagggtt
    11881 cccgtagaaa gaggctacac atagcctctt ttgctttttt cccctttctg ttataataag
    11941 tcaggtaact acattagaaa gatttgtggg tctgtcatct aagagatatc attcccgaag
    12001 aggtattagt gacttgtctc aatcaactgc aacttatatt aatgttattg gagctgggct
    12061 agctggttct gaagctgcct atcagattgc taagcgcggt atccccgtta aattgtatga
    12121 aatgcgtggt gtcaaagcaa caccgcaaca taaaaccact aattttgccg aattggtctg
    12181 ttccaactca tttcgtggtg atagcttaac caatgcagtc ggtcttctca aagaagaaat
    12241 gcgtcgcttg gattccatta ttatgcgtaa tggtgaagct aatcgtgtac ctgctggagg
    12301 agcaatggct gttgaccgtg aggggtatgc aaagagtgtc actgcagagt tggaaaatca
    12361 tcctctcatt gaggtcattc gtgatgaaat cacagaaatc cctgacgatg ccattacggt
    12421 tatcgcgacg ggtccattga cttcagatgc cttggcagaa aaaattcacg cgctaaatgg
    12481 tggcgacgga ttctattttt acgatgcagc agcgcctatc attgataaat ctaccattga
    12541 tatgagcaag gtttacctta aatctcgcta cgataaaggc gaagctgctt acctcaactg
    12601 ccctatgacc aaggaagaat tcatggcttt ccatgatgca ctaacaaacg cagaagaagc
    12661 accgctgaat gcctttgaaa aagaaaagta ttttgaaggc tgtatgccga ttgaagttat
    12721 ggctaaacgt ggcattaaaa ccatgcttta tggacctatg aaacccgttg gattggaata
    12781 tccagatgac tatacaggtc ctcgcgatgg agaatttaaa acgccatatg ccgtcgtgca
    12841 attgcgtcaa gataatgcag ctggaagcct ttataatatc gttggtttcc aaacgcacct
    12901 caaatggggt gagcaaaagc gtgttttcca aatgattcca gggcttgaaa atgctgagtt
    12961 tgtccgctac ggcgtcatgc accgcaattc ctatatggat tcaccaaatc ttttaaccga
    13021 aaccttccaa tctcgtaaca atccaaatct tttcttcgca ggtcagatga ctggagttga
    13081 aggttatgtc gaatcagctg cttcaggttt agtagcagga accaatgctg ctcgtttgtt
    13141 caaaagagaa gaagcgcttg tttttcctca gacaacagct attggtagct tgcctcatta
    13201 tgtgactcat gccgacagta agcatttcca accaatgaac gtcaactttg gcattatcaa
    13261 agagttagaa ggcccacgca ttcgtgacaa aaaagaacgt tatgaagcta ttgctagtcg
    13321 tgctttggca gatttagaca cctgcttagc gtcgctttaa cactaaaaaa gagttccttt
    13381 tcagttgagt tgctgaagag gacttttttc cttgctgatt tgagcgctag ctatgcaaaa
    13441 gaatgcacaa aaaatacatg aatagactat ggtcactaga agcaatatta tataatgaat
    13501 agtactcatt agttgattca gtttggaggt tttagcatgc agcagcaagt cgcaataaca
    13561 gaaacagttt tacgggacgg tcatcaaagt ttgatggcaa cgcgtttatc tattgaggat
    13621 atgcttcctg tccttaccat cttagacaag attggttatt attccctaga gtgttgggga
    13681 ggagcaacct ttgatgcctg tattcgtttt ttgaacgaag acccttggga aagactgcgc
    13741 acgttaaaaa aaggcttacc taatacccgt ttacaaatgt tattaagagg ccaaaacctt
    13801 ttaggctacc gtcattatgc agatgacatc gttgacaaat tcattagttt atcagctcaa
    13861 aatgggattg atgttttccg catttttgat gccctaaatg acccacgaaa tatccagcaa
    13921 gccttaagag ctgtgaaaaa gacgggtaaa gaggctcaac tgtgtattgc ctatacaact
    13981 agcccagtgc acacgcttaa ctattacctt tctttagtga aagaattggt tgagatggga
    14041 gctgattcta tctgcatcaa agatatggct ggtattctaa ctcccaaagc ggctaaagag
    14101 ttagtatctg gtatcaaagc catgactaac ttacctctta ttgtgcatac ccatgcaact
    14161 agtggcattt ctcagatgac ctatctagca gcagtagaag ctggtgctga tagaattgat
    14221 actgctttgt caccttttag cgaaggcact tcacaacctg ctacagaatc catgtattta
    14281 gccttaaaag aagcaggtta tgatatcaca ttagatgaaa cgctactgga acaggctgct
    14341 aatcaccttc gacaagtccg tcaaaagtac ttagcagatg gcattctgga tcccagtctt
    14401 ctctttccag acccaaggac actacaatac caagttcctg gaggcatgtt atctaatatg
    14461 ctatctcagc tgaaacaagc caatgccgaa tcaaaattag aagaagtttt agcagaagtt
    14521 cctcgggtaa gaaaagactt gggttatcct cccttagtaa caccgcttag ccaaatggtc
    14581 gggactcaag ctgctatgaa tgtgatttta ggaaaacctt accagatggt gtctaaggaa
    14641 attaaacaat acttggcagg cgactatggc aaaacaccag caccagtcaa cgaagactta
    14701 aaacgaagtc aaattggctc agctcctgtc accacaaatc gtcctgcaga tcaactaagt
    14761 cctgaattcg aggttttaaa agcagaagta gcagatttag cccaaacgga cgaagatgtg
    14821 ctgacttatg ccctcttccc aagcgtagca aaaccgtttt taacaaccaa ataccaaact
    14881 gatgacgtca ttaaagtgac tgcctttatc aaagcataat aggctatctt aatcagatag
    14941 aaacaaaaat atggagatca aatgacctat tcctttattg atatcttaca agttacctta
    15001 gtatccttgt tcttagtgtt aacctgttta acagggatta tgggattaat gatgctaagt
    15061 ggtaaattat tgtcacaaga aaaaaaaact ctctctcaac aagcttccag cattcctcaa
    15121 gagcaagaag ctgacaagtt gagtggagaa gctctcgaaa ctcttttagc caaagacgaa
    15181 ttggctagaa ttgctgttat gactagtctt gcttatgcta gccaagaaga gtcaggaaaa
    15241 cggtttgaag ttattgccgt ggaaaggaaa tcctaatgaa aagatatgaa gtgactgtca
    15301 atggacaggt ttatgaagtg agtttacttg aactagctga tggagaaact gttcaagtta
    15361 gccggccagc agcacctgct actgagaaag aaatgaatgc caacgctgct ggcggtggcc
    15421 tacaggtaaa ggctccaatg tctggaacag tcttgtctat ctttgctaca gaaggaaaag
    15481 ctgttaaaaa aggagaagct gttctggtct tagaggccat gaaaatggag aatgaaattc
    15541 tagcacctgc agatggtctg gtttcaaaaa ttcatgttgt ggctaaccaa acggtcgagt
    15601 ctgaacaagt tttgatttca ttctaggagg aaaccatgct agacgtactc aatcaaatgg
    15661 tccaatcgtc aggattggct catctaactg ttaataatct tatcatgatt tgcttggcct
    15721 gtttcttttt ataccttggt atcaaaaagg aatatgagcc ttaccttatg gtaccaatcg
    15781 cctttgggat tttactcgtt aatttgccta tggcgggatt gatggaccac cctgccaatg
    15841 gtaatcctgg tggccttctt tattacctat ataagggaac tagcctaggg atttacccac
    15901 ctttaatttt tctttgtttg ggggcatcaa ctgactttgg tcccttgatt gccaatccta
    15961 aaaccatttt actaggaggc gcagcacaag ttgggatttt cttagccttt ttccttgcta
    16021 ttatgttagg catgacacct caagaagcgg cttctgtcgg gattattggt ggtgccgatg
    16081 gaccaacagc tatttatgtg acgacaaagt tggcgcctga tttactctct actatcgccc
    16141 tagcagctta ttcttacatg gctctggtac ctattattca acctcctatc attaaactct
    16201 tgactaccaa ggcggaacgc caagtgaaaa tgactcaagc aagaactgtt tcacaaaaag
    16261 aaaaaatcat tttcccgatt atggtaacaa tctttgtcag ccttttggtt ccaagtgcta
    16321 caaccttggt tggctgtttg atgctgggaa atctggtcag agaaattaaa attgttccta
    16381 aaattgtaga gaatctccaa caagtggtta tgttttgcat tactatcatt ttaggattaa
    16441 ccgttggtgc taaggccaat ggagatctct ttttatcagc cacaaccttg aaaattattg
    16501 cccttgggct aatcgccttt gctgctggta cagcaggggg tgtcttgatg gggaaggtca
    16561 tgtactattt atctggtaac aaagtgaacc ctatgatagg agcagcaggt gtttcagctg
    16621 ttccgatggc agcgcgagtg gtccaaaagg tcggacaaga agaagatcca agtaatttct
    16681 tactcatgca cgccatggga cccaatgtag ctggcgttat tggctcagcc attgcttcag
    16741 gtgccttgct ggccttcttt ggctaattgt gaaaaaggat tagtaaatta atcttaataa
    16801 atcattatca ggagttggaa gcaatctagc tcctttttta gtagaaagct cgagtgataa
    16861 actattccaa aataattata aaaaaagact gtcctgattg tacggactat atcaattgac
    16921 ttttatagtc tagctgaaat agcagtgcaa aactaagaca ctcgttttaa agtagttttt
    16981 ctaaaaaagc aaaataaaag gtcagagcca ataaatctgc cgcgccccca gggcttaaat
    17041 gctgattaat gaggcattga ttgtaggagg acaaagccaa tcttaggtca gttgttgata
    17101 aatcttgttg caggagtagt cgcatatctg ctttaacgcc tttccatgct tcaatccctc
    17161 cacgatgaat cagatttcca tcttcaacaa tagacatcaa ataaacaaga agacgaagct
    17221 gactggtttc ggggtcattt tgacttatca tctgacgaaa gtagggcaag gcatggtctg
    17281 ttaaggtcgt gaatccctcg cttgcctcac cccgaggtcc tttgatgcca taagttacaa
    17341 agagttgctc cccataagtg aactcttttt tagtgttcaa atgaccaagg tctgtttgaa
    17401 tcaaatgtgc cgtcataggt tttactaaac gacagatagc tagagtatct tccttgctaa
    17461 aacaccctaa atcagtcatt aagtgaggtg ttcttgctag ataggcccca gttgcaccaa
    17521 gaagtaaagc catggaaaaa ttcaggcctt tatgagtgtt aattccctga gtagcacaaa
    17581 acatggtttc ctcggccttt tgacctagtt gtctaagacg gttaaacaga agaagtggct
    17641 cttcttttgc ataagcaaaa cctgtttcga tataagcctg aaaaaaagga gagagtgcta
    17701 tcatactgtc gataaaggtc ataaaggaca tgtcatcatg ggcaccgtta tcaaatcgat
    17761 caactaaacc aggttttgga gatagtgaga cttcataaag caaagctttt aaggccagtt
    17821 gactaatact tgttagaact gccttagtca ttttgctcag gcctcatttc ttttaagata
    17881 aattcaagtg agctattcaa gtgttcatgt gtaatcaacg taatttgctc atgatctttg
    17941 ttcttcatac ctcggtaaat catccagtgt tcagatagag ctcgtttacg gcgttcaact
    18001 gaagaaatcg aaatttttct aaagtagacc aaataagctt gcaattctgt cacaatttct
    18061 tttaagcgga ccatttggct atagctgtaa atcaaattgt taaaggcatt aaaattatcc
    18121 aaaatacgat tggtgtcatc ctctgcgata aaggtttcac agtcttccaa caagttatgc
    18181 atgactttaa aatcttcttc tgtcattaaa tgcatcgctt gcactgttgc gagagtctct
    18241 agagcttttc gaatttcaaa aatttcacag gcatccttga tactaactcc tttgacaata
    18301 atgccttttt taggaatgtg ctctacaagg tgttcttcag acaataatcc caaagcgtag
    18361 cgaataggtg tcctgctaat attaagcgca atggaaaact ctttttcatt aatgcggctt
    18421 ccaacaggaa tttgtcgcaa aataatggtc gttttaagag cgttataaaa ggctattttc
    18481 aaagggatat ttcttgatag gtcaagattt tgtttaacgg cttcgataat aggattcatg
    18541 tcatctcctt aatgctagtg tcctcacttt attatactta aatttgtgaa acattccaat
    18601 aggactagat gagttctgaa aaattaaagc catgctcaca aaaggacatg actttaatca
    18661 tctcataagg aataaggtag tagtgttatc gttggtttat tgtggaatgt aaagtggcat
    18721 ttgccccatg gcaataatgg ttaccacaaa gataacaaag atgataaggg catatttagc
    18781 agcctctttt tgccattccc ccatgtcaag acctgtcaag cgaagaagaa gataaatgaa
    18841 ggcaactaat ggactaagta agtggaaggc ttgtcccatg agggaagcaa gtgccatagc
    18901 catgttactg aaaccatatt cagcaccagc ttctgcaaga acaggcaaga taccatagta
    18961 aaaaccatca ttggagataa agaaggtacc aggtgcagaa actaaggcaa taacaagacc
    19021 ccagaaacct gccagttgtt ttggaataat ggttgcaaaa ctttgtgcaa gagcgctagc
    19081 cataccagaa ccttggaaaa gacccataaa gataccagca gcaaagacta agataaccac
    19141 ttgaaccgca tcaccagcat tgtcaccaat gcgttttgat tgatctttaa gtactgggta
    19201 attgaccatc aaggcgatac aagttcccac cgcaaaaaga agaagcggtg ccatagcaat
    19261 agatttgttg aaagagccag caactaacca agtgattaat ccaatggtta ggatagcatt
    19321 gaaaacaaag tttttaggac gacgaataaa aagggtatct gggtcagtaa tgtctgtgag
    19381 ttgacgcatg tcttcttcag acaagtcaat aacaccaagt cttgcacgtt ctttacgccc
    19441 catgcttgga gcaacccaac aaatcacata aagaagagat aaaatcatac ccggtgcaag
    19501 atagccaaga atttcaggac cgacaccaag aacagacatc gcacgagcag tagggccacc
    19561 ccatggcagt aagttcataa tagtattttg aagaataatc aagacaccta ggttcatgat
    19621 tttcatgtcc aattttttat agataggtaa gaaagcagag caacaaatta aagtagtggt
    19681 tgttccatca ccattaagag atactgcagc agcaacgaca gctgttgcga taagcacctt
    19741 cattggatca cctttagcaa agcgaatcat cttttcagta atcggatcaa acaatcctgc
    19801 atctaacatg acagagaaga acaaaatggc aaatagcaac atgatacctg ttttagcagt
    19861 attgttgata ccatacataa ccattggtcc gatagctgtt aggtctttag caattccacc
    19921 gtcaccaaca aaggcaacaa atttggcatc tgcattgaag tcagctgaac cagtcagtat
    19981 aacagcaatc gtcatgatta atggaatcat gaccaaagca gtaaaagggg tcattttttt
    20041 cttcattacc acgtacataa agacaataat catggcataa gccagcattg ttaataacat
    20101 cattgttatc tcctttttca agttatgatt tcagtataga tgaaagcgct tataaaat
//
LOCUS       DATUYR010000030        20067 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.030, whole genome shotgun sequence.
ACCESSION   DATUYR010000030 DATUYR010000000
VERSION     DATUYR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20067
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.030"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            149..1117
                     /locus_tag="VCI33_001035"
     CDS             149..1117
                     /locus_tag="VCI33_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEP2831965.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            1176..2174
                     /locus_tag="VCI33_001036"
     CDS             1176..2174
                     /locus_tag="VCI33_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEP2831966.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            2359..3768
                     /locus_tag="VCI33_001037"
     CDS             2359..3768
                     /locus_tag="VCI33_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEP2831967.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKADVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLAEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            4099..5862
                     /gene="lpdA"
                     /locus_tag="VCI33_001038"
     CDS             4099..5862
                     /gene="lpdA"
                     /locus_tag="VCI33_001038"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEP2831968.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(6535..8952)
                     /gene="hylA"
                     /locus_tag="VCI33_001039"
     CDS             complement(6535..8952)
                     /gene="hylA"
                     /locus_tag="VCI33_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEP2831969.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQMLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASANITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSSIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTSVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYHAYVNNQPVDLNNQLVDFTNTKSIFLESDDSAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATMFSNHNLSHQGFYSFPH
                     PVKQNQQ"
     gene            9185..10174
                     /locus_tag="VCI33_001040"
     CDS             9185..10174
                     /locus_tag="VCI33_001040"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP2831970.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFECLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            complement(10287..11078)
                     /locus_tag="VCI33_001041"
     CDS             complement(10287..11078)
                     /locus_tag="VCI33_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEP2831971.1"
                     /translation="MTYTSLKSPENTDYLYDLNIAHLYGNLMNTYGDNGNVLMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTSLKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(11078..12421)
                     /locus_tag="VCI33_001042"
     CDS             complement(11078..12421)
                     /locus_tag="VCI33_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEP2831972.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GRQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIMEAIEKQESKHAYILATYTAMLEFRSLLADRHVVEKEMN"
     gene            12528..13379
                     /locus_tag="VCI33_001043"
     CDS             12528..13379
                     /locus_tag="VCI33_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEP2831973.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            13376..14332
                     /locus_tag="VCI33_001044"
     CDS             13376..14332
                     /locus_tag="VCI33_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEP2831974.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVNVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            14386..15741
                     /locus_tag="VCI33_001045"
     CDS             14386..15741
                     /locus_tag="VCI33_001045"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEP2831975.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            15865..16506
                     /locus_tag="VCI33_001046"
     CDS             15865..16506
                     /locus_tag="VCI33_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2831976.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTPKQDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQENLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSRP"
     gene            16569..17699
                     /locus_tag="VCI33_001047"
     CDS             16569..17699
                     /locus_tag="VCI33_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEP2831977.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGVSFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            17709..18461
                     /locus_tag="VCI33_001048"
     CDS             17709..18461
                     /locus_tag="VCI33_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEP2831978.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKPHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESETD"
     gene            18461..19225
                     /locus_tag="VCI33_001049"
     CDS             18461..19225
                     /locus_tag="VCI33_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEP2831979.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQERGIPYLLVTNN
                     TTRTPEMVQSMLANQFHVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            19225..19857
                     /locus_tag="VCI33_001050"
     CDS             19225..19857
                     /locus_tag="VCI33_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEP2831980.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT         6194 a         3569 c         4182 g         6122 t
ORIGIN      
        1 attttttagt acagttttgt ttgtaatttc acaaactatt gcatttctaa aaaaccaaat
       61 aaggtaaggt agaaagcgat ttaattattt gcaaattgtg aaaaaaagtt ttatcataaa
      121 aaggtaatat gataatatgg aggccgaaat ggtaacagtt tctaaagaac aacatttgga
      181 tatgttcctt aaaatggaac gtattcgtga atttgattca cgtattaata aactcgtccg
      241 tcgtggattt gtacaaggca tgactcactt ctcagttggt gaagaagcag ccaatgttgg
      301 agcggtggct cacttgtctt acgatgacat tattttttca aatcaccgtg gacatggaca
      361 atcaatcgct aaagacatgg accttaataa aatgatggct gagcttgctg gtaaagcgac
      421 tggtgtttct aaagggcgtg gtggctcaat gcacttggct gattttgaaa aaggaaacta
      481 tggtaccaat ggtatcgttg gtggtggtta cgctcttgca gttggtgctg ccctcaccca
      541 acaatacaaa ggaactaaca atatagctgt tgctttctca ggtgatggcg ctactaacga
      601 aggatcattc cacgagtctg ttaatatggc tgcaacatgg aaacttcctg ttattttctt
      661 catcatcaac aaccgttatg gaatttcaat gagcatcaat aatgcaacaa atacaccaca
      721 tttatacaca cgtgcagaag cttacggaat tcctggtttc tactgtgaag atggcaatga
      781 cgtgatggct gtctatgaaa caatgggcaa agccgttgaa catgttcgtg gtggaaatgg
      841 tccagctatt gttgaggtag aatcataccg ttggtttggt cactcgactg cagatgctgg
      901 taaataccgt actaaagaag aagtggatga gtggaaagaa aaagatccaa tgatcaaata
      961 tcgtacttat ctgacaagcg aaggcattgc cactgatgac gaattggatg ctattcaagc
     1021 acaagtcaaa aaagaagttg acgatgctta tgaatttgca caaaacagtc cagatccaga
     1081 actttcagta gccttcgaag acgtttgggt tgactaagat caccttgtca gtaagaagca
     1141 atcgtatttg aataataaat ggagaaataa aaatgatgtc agaaacaaaa ttaatggctt
     1201 tgcgtgaagc agtcaacctt gctatgactg aggaaatgcg caaggacgaa aacattttcc
     1261 ttatgggaga agacgtaggg gtttacggag gagacttcgg gacttctgtt ggaatgattg
     1321 aagaatttgg tcctaaacgt gttaaagata ccccaatttc agaagctgct atctctggtg
     1381 cggctattgg tgcagctatt acaggactac gtccaattgt cgacgttacc tttatggatt
     1441 tccttaccat catgatggac gctatcgtga ataatggtgc taaaaataat tacatgttcg
     1501 gtggaggcct tattacccct gtaaccttcc gtgtggcatc aggttctggt attggttccg
     1561 ctgcgcaaca ctcacaatct cttgaagcat ggttgactca cattccagga atcaaagttg
     1621 ttgctcctgg taatgccaac gatgctaaag gcttattaaa atcagctatc cgtgataata
     1681 acattgttct tttcatggaa ccaaaagcgc tttatggtaa aaaagaagaa gttaaccaag
     1741 atcctgactt ctatatccca cttggcaaag gtgacatcaa acgtgaagga actgatttga
     1801 caattgtgtc atatggtcgt atgttggagc gtgtccttca agcggctgaa gaagttgcag
     1861 cggatggtat taacgttgaa gttgttgacc cacgtaccct catcccactt gataaagagt
     1921 tgattattga atctgttaag aaaacaggta aattgatgct tgtcaacgat gcctacaaga
     1981 ccggtggatt cattggggaa attgcaacca tgattactga aagtgaagcc tttgattacc
     2041 ttgatcaccc aatcgttcgt cttgcaagtg aagatgttcc agtaccttat gcacgtgtac
     2101 ttgaacaagc gatcttacca gacgttgaga aaatcaaagc tgccattgtg aaaatggcaa
     2161 acaaaggcaa ctagaagata aggcaagtgg tctgataaag gctatgttta tcagtaacgt
     2221 ctcagctaaa aactattttg aaagttttta gaagaactga gttgtcttat cccgtgtcgc
     2281 ggcacctcac ctttcgtatg ttttcgtagc aaggtgggga tgttgatggc gcttatcaaa
     2341 tcgtgaaagg atgtagaaat ggcatttgaa attattatgc caaagctggg cgttgacatg
     2401 caagaaggcg agatcatcga gtggaaaaaa caagaaggtg ataccgtcaa tgaaggtgat
     2461 atccttcttg aaatcatgtc agacaaaacc aatatggaac ttgaggcaga agactcaggt
     2521 gtgctattga aaattgtccg tccagctggt gatactgtac ctgtaactga agttatcggt
     2581 tacattggtg cagagggtga atcagtagat actattgctt caagtgaaaa aacaacagaa
     2641 attccagttc ctgcttcagc agacgctgga ccagcagttg ctccaaaaga aaatgtggca
     2701 agtccagctc ctcaagtggc agcgacagct attccgcaag gaaatggcgg aaaagttcgt
     2761 gctactccag ctgcacgtaa ggcagcagca gaaatgggca ttgaccttgg ccaagttcca
     2821 ggaacaggac caaaaggacg tgtccataaa gcagacgttg aaaactttaa aggtgctcaa
     2881 ccgaaagctt caccacttgc tcgtaaaatt gcagcagaca aaggtattga tttggcaacc
     2941 gtatcaggta ctggctttaa cggtaaggtc atgaaagaag atatcatggc tattcttgaa
     3001 gcggctaaac cagctgaagc aaaagcccca gcagctaaag aagaaaaagt tgttgatctt
     3061 gcagaaggtg tggaacacaa gccaatgtca gctatgcgta aggcaatctc taaaggtatg
     3121 acaaactctt accttactgc tcctaccttt acgcttaact acgatattga catgacagag
     3181 atgattgccc ttcgtaagaa gttaattgac ccaatcatgg ctaaaacagg ccttaaagtc
     3241 agcttcacag acttgattgg tatggcagtg gttaaaacct tgatgaaacc tgagcatgag
     3301 tacatgaatg cttccctcat caatgatgct aatgatattg aattacaccg ctttgtgaac
     3361 cttggtattg ccgtaggtct tgatgatggt cttatcgttc ctgttatcca tggggctgac
     3421 aagatgagct tgtcagattt tgtgcttgct tcaaaagatg tgattaaaaa agcgcaaact
     3481 ggtaagttga aagcagctga aatgtctgga tcaaccttct ctatcacaaa cttgggaatg
     3541 tttggtacga agacatttaa cccaattatt aaccaaccaa actcagcaat tcttggtgtt
     3601 ggagcaacta tcccaactcc aacggttgtg gatggtgaga ttgttgcccg tccaattatg
     3661 gcaatgtgcc taaccatcga ccaccgttta gttgatggta tgaacggtgc aaaattcatg
     3721 gttgatctca agaaattgat ggaaaatcca tttgaattgt taatctgatc aatcagtttt
     3781 aaacaagaaa agcattcaat aataaaaggg attttgctaa gtttagactt ggtgggtgcc
     3841 cttttctcaa aaatatagaa aggatgtaga acgaggagat ctcatgcaaa tgagtgataa
     3901 gcaattgaat ccgagcgaaa agctcggata aaaatggtaa gagttggcta atcttagcgt
     3961 cgtagacgtc tgaactttcc tcaatttttg tttcggaaag tcctagtcgt cctgacgtgg
     4021 gtgcgtcaac gaaatcaaag atttctgact taccgcaaaa attcaatcgc tttctccttg
     4081 ctcttggtat cttaaattat ggctgttgaa attattatgc caaaactcgg tgttgacatg
     4141 caagaaggtg aaatcatcga gtggaaaaaa caagaaggtg atactgtcaa tgaaggcgat
     4201 attcttcttg aaatcatgtc agataaaacc aatatggaac ttgaagcaga agactcaggt
     4261 gttcttctta aaattacacg tcaagcaggt gaaacagtac ctgtaacaga agttatcgga
     4321 tatatcggtg ctgaaggtga atctgttgag gtttctagcc cagctgcttc agatgtgaat
     4381 gttgctcgta caacagaaga tttagaagct gctggacttg aagtgccaaa agcaccagct
     4441 caagctgctt cagctgcacc aaaagctgca cttgctgatg atgagtatga catcatcgtt
     4501 gttggtggtg gccctgctgg ttattatgca gctattcgtg gtgctcaact tggtggtaaa
     4561 attgccatcg ttgagaaatc tgaatttggt ggaacttgct tgaacgtagg gtgtatccca
     4621 accaaaactt accttaaaaa tgctgaaatc cttgatggta ttaagattgc agcaggacgt
     4681 gggattaacc ttgcctcaac taactatacc attgacatgg acaaaacagt tgactttaaa
     4741 aacaccgttg ttaaaacctt aacaggcggc gttcaaggtc ttctaaaagc taataaagtc
     4801 actatcttta atgggcttgg tcaagttaac cctgacaaga ctgtaaccat tgggtcacaa
     4861 accattaagg gtcgcaacgt tatccttgct acaggttcta aagtatcacg tatcaatatc
     4921 ccaggaattg actctaaact tgtcttaacg tctgacgata tccttgacct ccgtgaaatg
     4981 ccaaaatcac tagcagttat gggcggtggt gttgttggta tcgagcttgg acttgtttgg
     5041 gcatcttacg gtgtggatgt taccgttatt gaaatggctg accgcattat cccagctatg
     5101 gataaggaag tctctcttga acttcaaaaa atcctttcta agaaaggcat gaagatcaaa
     5161 acatctgttg gtgtctctga aattgttgaa gcaaataacc aattgacctt gaaacttaac
     5221 aatggtgaag aagttgtcgc tgaaaaagct cttctttcta tcggtcgtgt accacaaatg
     5281 aacggtctgg aaaatcttaa ccttgaaatg gatcgtaacc gtatcaaagt taatgactac
     5341 caagagacat caattccagg tatctatgcg ccaggtgacg ttaacggaac gaaaatgctt
     5401 gctcacgctg cttaccgtat gggtgaagtg gctgcagaaa atgcgataca tggcaacaca
     5461 actcgtaaag ctaaccttaa atacactcca gcagccgttt acacacaccc tgaagtggca
     5521 atggttggtt taactgaaga acaagcgcgt gaacaatatg gtgatgtgct tattggtaaa
     5581 aacagcttta ctggtaatgg acgtgcgatt gcttcaaatg aagcccatgg ttttgttaaa
     5641 gttattgctg atgccaaata ccacgaaatc ttaggtgttc atattattgg tccagctgca
     5701 gctgagatga ttaacgaagc agctactatt atggaatctg aattaaccgt tgatgaattg
     5761 ttattatcaa ttcatggaca cccaaccttc tctgaagtga tgtacgaagc ctttgcagat
     5821 gtgcttggcg aagctatcca taacccacca aaacgtaagt aaaacgtctt tgtcttatta
     5881 actaatgaaa aatatcatca cataacagat cacaaaaaag actagcacaa ctatgaactg
     5941 caccccaaac gttagatata aaaccctaac gtttggggtg tttttgtatg acattaagct
     6001 atgaagataa gctagacatt tagcgctaaa aaaacgagag aactccgttt gaaagaagca
     6061 tctctgaaaa gaagaagaca aaaaaccatt ataaagaatg gacaaagacg agctctgatt
     6121 agattttctt ttaaggacgg ctaaagtgac tcgttctacc tactattatc atcgattatt
     6181 gcaccaataa ccgtatcaaa ataaaacgaa aaggacttag tcctctacaa tacagaacta
     6241 aatcctttac ttcaaataga gtagccaact tttggggctc agtgcagttt ccagtttttt
     6301 gtctttaata acgtttcatt gttaaccctt actttccaat aacagctaaa tccttaagtc
     6361 tttcttacga aagggttttg tgatatggtt aaaagaagca actaaagctc cacctaagaa
     6421 ttctaaagag gaggactgat acccagtatg cggtaagcgt cgttgtcttt tgtggggaat
     6481 tttatgagag ttaagagcat tgtttttagc tgcaattcct tgatgagcaa gcttttattg
     6541 ttgattttgc ttgacaggat gaggaaaact ataaaagcct tgatgagaaa gattatgatt
     6601 tgaaaacatc gttgcttttt tttcatagtg aatgacgtgc atctgttgac tatcatgatc
     6661 gtagacagcg gccagtttgt cattgttttc taacaagtcg atatcaagct tgctaatata
     6721 ggtttcaaat tcttgacgag tcatatttgg aagcatcata taggcataac gatcgccatc
     6781 ttgagtatgg ttttgcatga tagtgataaa ggtatttgaa acttctttga tggcttctgg
     6841 tgatttgtca tcagctttta tgttttgcca tttccctgtt tgaagtgcct tacttatgct
     6901 aagtgttgtt ggcttgaaga agtagtaacc aatattttga gcagaatcat cactttcaag
     6961 gaaaatactt ttagtgtttg taaaatcaac tagctgatta ttcaagtcaa cgggttgatt
     7021 gttaacataa gcatggtaag ggtgcttttg attttctttt cgttgttcaa tagttgtata
     7081 cgccttgtga gatgattggt ttttgatatt gctaccaaca aagattattt tgtttcctaa
     7141 gataaaccac cctttattga gagtgaggct tttattccaa ttagtgaagg tcatagcagc
     7201 tagagcactt gtgttattaa gttttttact tgcaacaaaa gcgtcatcag agagactagt
     7261 catgccaact tgttgatagt tcgttttaat attctcagga gttccctcta gtggtttttg
     7321 ctcagtttct gtggttccag gtaagcggta gggatttaca gttgcccaat agttttcact
     7381 gtagtgtcct aaatcgttat tgtataggta aaacattcca tcagaagtaa accagccatg
     7441 aagattttca ttattcatag cttcataatt ttgagttcga ttcgaaaaca ttgataggcc
     7501 aaaagcaaaa tcgtgtttat tattatatag tgccaattta tccatactat tgaaactagc
     7561 tacgtaacta tcaagttttt ggactggaac agaagtatca cttagcagtt ctttcataag
     7621 tttgatatcg tgataggttt tcaaattatc atagacattg taaaaagcat tcccttgtgt
     7681 gacgagtgtt tttatacgtg ttttaagtgc caaacggtga ggctcttcag acatgtcagc
     7741 aatacgtaaa atagcacgaa gtgcttcaat gccagcaaca tgagattgag cattaaaacg
     7801 actgatagaa cgccctcgag tcatatccat catttctcca cgaacgatga tagggaaaaa
     7861 agaatggtta atccaatgat agatagtagc cattttatcc gcttctatag aagactttgt
     7921 tttttgaata ataggaatta attgcgataa gccatctata agaacattac cgtaagctcc
     7981 agtgtaagca atgccttttt tataaagtgg actttgagcg ttagtaacca cgtgatcaat
     8041 taaagaaccg tcttggtaaa aaccatttcc ttcgtcaact agcgtgaaaa ctttctcaat
     8101 tgctttgatt gtatcactaa tttcgagatc atctttacga agaataccgg aaatgagttt
     8161 aacacgcccc atatcaatta aatttccgct attggcttca aaaggtgaaa aattggcagc
     8221 gcgaacccta aaacgagtag ggtcaggcac aaatttttcg attggagctg tgtatttaag
     8281 gatttcttct tgagtaaaat aaggatacat caaggataag gtattattaa tagcacgagg
     8341 agttccaatt tcataaaccc accaattttc tttgttttct tttccagttg tttgatgatt
     8401 ttcacgatct agattataag cgtgttcata catgaaggcc ataccgtctt ttacaatagc
     8461 aatagcttta ctatcttgat aatagcatga ctcaggatta gtgatctgtt ttgctatttt
     8521 ttcaatattg cggtaagttt tcgtgatatt agcagaagcg gaataatcct ttgcatgttc
     8581 ccaaaggtaa gttctatttt cgtggtcagg cccttgatag cttttaataa tgttttgagc
     8641 atccttttca gctttattat gaaaagtgac catgtcagga ttggttttat cataagcatc
     8701 gttgcctgcg ataatactgt tccattggtc taacaattca gtatagtagt ctttttgttg
     8761 tggctgtact accttttttt cgctatctgt agtagtgagc atttgcgttt gaaagtaagt
     8821 attattaggt tctgaatttg aagtcagtgt atcggcataa atggcagttc cttggcccat
     8881 catagcaaag ctaaggaata ggtttgaata aagtagtaat tgtttatggt gtgtgcaaaa
     8941 ataagtattc actgataaat tcctcctata taaaaatgag ataataaaag taaattggtt
     9001 tactaagtaa aaatatacca cgttaaagta tttttataaa cccttacata aataatggct
     9061 gagtttttgt gcactaataa gtgtaggtac tgaagatgtc atacagaata ggacacttgt
     9121 cttttatatt tgttttcatt atactagaaa ggtagattac atttttgaaa ggattttttc
     9181 tcctatgaaa tacatcgtta acaaaagtca caatcctgcc tttaacatcg ctttggaagc
     9241 ctatgctttt agagaactag tggaagaaga cgaattgttt atcctttgga tcaatgagcc
     9301 agctattatt attggcaaac atcaaaacac cattcaagaa atcaataaag aatacattga
     9361 tgaacatggt attcatgttg tgcgacgttt atctggtggt ggtgcggttt atcatgattt
     9421 gaataacttg aactacacta tcatttcaaa taaaacagcg gaaggcgcat ttgatttcaa
     9481 aaccttctca caacctgtga ttgcgacttt agctgatttg ggtgtaacag ccaactttac
     9541 aggccgtaat gatattgaaa ttgatggcaa aaaaatctgt ggtaatgctc aagcttacta
     9601 caaaggtcgc atgatgcacc atggttgcct tttgtttgat gtggatatga ccgttcttgg
     9661 cgatgctctt aaggtaagta aggataagat tgagtctaaa ggagtgaagt ctgttcgtgc
     9721 tcgcgtgacc aatattttaa atgaattacc agaaaaaatt accgttgagg aattttcaga
     9781 taaaatcttg accaagatga aagaaacgta tccagacatg acagaatatg tcttgtcaga
     9841 ggatgagttg gctaagattg aacaatctgc caaagagcaa tttggtagtt gggattggac
     9901 ctacggcaaa gcgccagaat atacgattga acgcaatgtt cgttaccctg caggtaaaat
     9961 ttcaaccttt gcaaatgttg aaaattctat cattaaaaac cttaaaatct atggtgattt
    10021 ctttgggatc aaagatgtgc aagacattga gaacttatta attggttgta aatatgagta
    10081 ccgtgatgtt tttgaatgtt taaaaacgat tgatacgacc cagtacttct cacgcatgac
    10141 cgttgaagaa gttgctaaag ctattgtttc ttagtggttt taatcactta agaagctctg
    10201 tcagaagaaa aaactataaa actcctaagc gaactatcta tcgtgtcctg cttaggagtt
    10261 tttgtttgat gacatgaaag aagaacttaa actttattaa aggaagcttt gcttttaagg
    10321 tcggcatatt cttcagtgat ttcttgtttc agtatatcat catagctagg tagtgaaata
    10381 gctgatccat actttttttt aagagatgtt gttactaagc ggtaagcaag attaacattg
    10441 cgtgataaga taggaccgtg gaaataacta ccatagacgt ttttatagtg aacaccttca
    10501 gtttggtctt ctttattatt accgttgcca taaaccactc gtccaagtgg cttttcgtca
    10561 ccagataaga aggtacgtcc ttgatgattt tcaaagccat aataggtttc gttaaattcg
    10621 tcattatgaa ttttaatatc tccaataaag cgattttggt gctgattaag ggtgtagtgt
    10681 cccataattc caagtccatc aattttaacc ccattagctt ggacatagta ttgccctagt
    10741 aattggaagc caccacagat ggcaagcacc actttattat tagcaatgta atctgctaag
    10801 gcagcttttt tgctaggcaa atccttggca acaatgcttt gttcataatc ttgaccacca
    10861 ccaaagaaaa caatatcgta atcgtcttgt tcaaaggtat cattgataga tacaatatca
    10921 acagtaaccc tagctccaag tttttcagcg acatacttga gcatcaagac attcccattg
    10981 tcaccgtagg tattcatcag gtttccgtag agatgagcaa tgtttaggtc atagaggtag
    11041 tcagtgtttt ctggagattt tagtgaagtg taagtcatta gttcatctcc ttttcaacaa
    11101 catggcggtc ggctagcaag ctacggaatt ctaacattgc agtataggtt gctaaaatat
    11161 aagcatgttt gctttcttgt ttttcaatag cttccatgat ttgctcgagc ttttcagctt
    11221 gttttatctt agtgtcgtca aatcctgtga ctctaagtct tctggcaatt tcagagtgac
    11281 ggacgccgcc agcattaatt tctgtaatgg gcatttgggt aatgagttca aaattagcat
    11341 cccaaatcca gctggtatca atgccgtcag cgtaattagc atttaaaagc acagataagc
    11401 taaatgggta atcagctagc tgaatcattt caagcgcttg actggctcca acaggattct
    11461 tgataaggat aagggtgcat gatttatcac caatagtaaa agtttcttgt cttccaaaaa
    11521 ccgccttact tttgttaaag cccgccttga ttttctcggg agaaacaccg aaaaattcag
    11581 caacagaaac cgcagcaagc gcattgtaaa tattataaag accaccaaca tttatcttgt
    11641 agttttgtcc atcaataaca aattcagaac tttggtgagt aatagcggtg agttcagtta
    11701 atcgataatc caactgtggt ctcttgaagc cacaatttaa acaaataaag tctcctagat
    11761 tcgcataagt attgagacga tactgaagaa tatgttcgca ttttggacat aaaatccctt
    11821 ctgtgttgta atgagctagc tgcggagcat gcttagcagt atcaaaacca taatactgaa
    11881 ctggattaac tatgtcctta gaagagaaaa taggactatc tccattggct agaatagttg
    11941 cttttggagc atttcgagca ccatctacaa tcatctgata agtagtataa atctctccgt
    12001 agcggtccat ctgatcccta aaaatattag tataaacaaa aagactaggt tttaagtaag
    12061 tggtaattct aggaagacta gcttcgtcaa tttctaaaac agcaatttgc cttccagatt
    12121 tccctttttt agcagctaaa aaagtagaag taattccagt aatcatattg gcaccacttg
    12181 gatttgtgat tatctcgcca aatgcctctt tcaaaatacc gacagtaaga gcagttgtta
    12241 acgttttacc attagtacca gtgacgacca caatatcata gtccttagac aaatccttta
    12301 aaatatcttt attacaagct agagccaatc gaccaggata agtagatcct cgccctagtt
    12361 ttgttaaaat ggattgagca gctttgccag caataatgcc gagtaatgtc ttcattttca
    12421 tgcttatatt ttatcataag tttattgatt ttagataggg aaaaatcttt gatcatgata
    12481 tgatataata tagctaataa cttttttgat atagaaatga gagttgtatg aataatttat
    12541 ctagtatcga tattaaattt ttattaagct tatttgcgga tccttggctg ctggcagtgc
    12601 atcttttaga tattctaatt gttgcctatt tgatttatcg ttttattaaa gcattgactg
    12661 gaactaaaat catgtcttta gttcaagggg tcattttctt tttggttctt cgttttattg
    12721 cagagtggat tggctttacg acaatcacat acttaatgaa ccaagtcatt acttatggtg
    12781 taattgcagg agtagtcatt tttacccctg aaattcgggc aggactggag aaatttggtc
    12841 gttctacgca agttttttta caaaaacagt acgtcagcag tgaatctgct ttggtcgatg
    12901 ctttgattaa atctgtggcc tatatgggtc cacgcaagat aggtgcattg attgctattg
    12961 aacagactca aaccttacaa gaatatattg cgacaggaat tccgctgaat gccgacattt
    13021 caagtcaact actcatcaat atttttattc cgaatacgcc gcttcatgat ggagctgtta
    13081 tcgttggtca gaataagatt gtggcagcct gtgcttactt gcctttgtca gaatcaaagg
    13141 caatttccaa agaatttggg acccgtcata gagcggctat tggattatct gaaaactctg
    13201 atgcactaac tatcattgtt tcagaagaaa caggagctat ttcagtaact cgcaaaggcc
    13261 aatttttaca tgatttaagc acagatgaat ttgaaacggt acttaggacc tacctgatga
    13321 gtgattcaaa tgttagttta ccatggtata agaaaattct aggagggaaa tctaaatgaa
    13381 acgattttta aatagtcgtc cttggttagg aatggtatca gtcttttttg cgattctcct
    13441 ttttttaact gcagcatcga gcaatcataa taattctagc tcgcaaattt atagcccgat
    13501 tgaaacctat acgcatagcc ttaaagatgt accaattgat atgaagtatg atagtgacaa
    13561 atattttatc agtgggtatt cttatggtgc ggaagtttat ctaacctcca ctaatcgtat
    13621 caaattagat tctgaagtga acaatgatac acgtaatttt aagattgtgg cggatctaac
    13681 acatagtcat ccaggtacag taaatgtcaa cctaagagta gaaaaccttc cttctggggt
    13741 gacagctacc gtatctccag ataaaatatc agtcacaatt ggcaaaaaag aatctaaggt
    13801 atttcctgtt agaggaagcg ttgatgctaa acaaattgca aatggttatg aaattagcaa
    13861 aattgaaacg ggggttaata aggttgaagt gaccagtgat gaatctacca ttgctttaat
    13921 tgatcacgtt gtggcaaagt tacctgatga tcaagtgtta gataggaatt atagtagtcg
    13981 agtgacctta caagctgtct cagctgatgg aactatttta gcaagtgcaa ttgatcctgc
    14041 aaaaacaaat ttatcagttg ctgtaaaaaa aataacgaaa tcagtcccga ttagagttga
    14101 ggcagtgggg atgatggatg atagcttatc agatattcaa tacaaattgt caaaacaaac
    14161 agctgttatc tcaggtagtc gagaggtctt agaagatatt gatgaaatta ttgcagaagt
    14221 taacatttct gatgttacta aaaatactag taagaccgtg agtttgtctt ctagtcaggt
    14281 gtcaattgag ccgtcagtag tgaccgttca gttgacaacc accaaaaaat aatggataat
    14341 aactaggaaa attgaatgga ggaagccatt aggcttatat aaattatggg aaaatatttt
    14401 ggaacagatg gtgttcgtgg cgaagcaaat gttgagctga cacctgagtt agcttttaaa
    14461 ctgggccgat tcggtggtta tgttttaagt caacatgaga ccgaaagacc taaagttttt
    14521 gtggcgcgtg acacccgtat ttcaggagaa atgttggagt cagccttgat tgctggtcta
    14581 ctatctgttg gtattgaagt ttataaactt ggcgttttag cgacacctgg tgtctcttat
    14641 ttagttagaa cagaaaaagc aagtgcaggt gtgatgattt ctgcaagtca taaccctgcg
    14701 cttgacaatg gcatcaaatt ctttgggaat gatgggttta agttagctga tgaccaagaa
    14761 ttggaaattg aagctctctt agatgctccg gaggacacgc ttcctcgccc gagtgcagaa
    14821 ggacttggaa ccttggtaga ttatccagaa ggacttcgta aatatgaaaa gttcttggta
    14881 acaactggta ctgacttaag cggtatgaca gttgccttag acactgctaa tggagctgct
    14941 tcagtgtcag cacgtgatgt ctttttagat ttaaatgcag aaattgctgt cattggtgaa
    15001 aaaccaaatg gtcttaatat taatgacggt gttggttcaa cgcatccaga acaacttcaa
    15061 gagttagtta aggaaacagg agcagatctt ggtttagcct ttgatggcga tagcgaccgt
    15121 ctgattgccg tggacgaaac tggcgagatt gttgacggtg atcgtattat gtttatcatt
    15181 ggtaaatact tgtcagaaaa agggcttctt gctcacaata ccattgtcac aacggtgatg
    15241 tctaacctcg gttttcataa agctttagat aaacaaggta ttaataaagc aattacagca
    15301 gttggagacc gctatgtggt tgaagaaatg cgtagctcag gatacaatct tggtggtgaa
    15361 caatcaggcc atgtcattat catggactac aataccactg gagatggtca gttgacagct
    15421 atccaattag ccaaggtgat gaaggaaact gggaaatcac tttcggaatt agctgctgaa
    15481 gtaaccattt accctcaaaa actggtgaat attcgtgtgg aaaacagcat gaaagaccgt
    15541 gctatggaag ttccagctat tgcgaacatc attgcaaaaa tggaggatga aatggctggt
    15601 aatggtcgta ttctcgtgag accaagtggt acagaacctc ttcttcgtgt catggcagag
    15661 gcgccaacag atgccgaagt tgattattat gtcgatacta ttgctgatgt cgtccgtaca
    15721 gaaatcggat gcgataacta acgagttgag tacaaacaag aaaccttatt tctaaggctt
    15781 ctgtcaacaa ttggaaagag gcttggggcg tttagcccaa gtctcttttt ataaattgct
    15841 aaagtagaag agaaaggata tcggatgaca aaacagcaaa tcgaaaaact agcggactta
    15901 ctgcctcctt ttgcagccac actttactta gatatgattc ctgaatttca ggaaggttat
    15961 gcttcatatg tttatcaaac accaaaacaa gatattcaaa agcgccgcat caagcagtta
    16021 actaaaaatt ttgaacgatt actagatacc ccatcttttt atgtgatgcc tgtgacaaga
    16081 aaaacagcag agcaattgct agcagataaa ggcctagctt atcatttgtt atcgacagtt
    16141 gcagctaatc aagtgtttgc ggctgctgtt aacgaggatc agacgctttt tcaagtgctt
    16201 cgaaatggag caagagtagc ctttgtgatt cttcaagaaa agggggaatt tctagaaatg
    16261 actttaggga ttcttgaaac agtgacagaa aacgaggttc cagcggctat tgtggaggct
    16321 gtccagacat atcttctggc agatatgcaa gagaacctcc cttctttaag cattatggtc
    16381 agtcaagaat caagcaagct tttttaccaa gaacaaggat ttgttttgct agctaagggt
    16441 tgcaagccag gaaacgaaaa tgattctgac gtttgtcagc tcgtaaaata tttttccagg
    16501 ccatgaagaa tcaagggaaa tggcttattc atagaaacat tttatcatca aagtggtata
    16561 ataagaccat gtcaaaaaaa cctacttcag cctatgtgca cattcctttt tgcacacaaa
    16621 tttgttatta ttgcgatttt tcaaaagtct ttatccaaaa tcaacctgtt gatgcttatt
    16681 tgaaagcctt gattcaagaa tttgactctt atggcattcg agacctaaaa accctttata
    16741 ttggaggagg tacgcctaca gctatcactg ctaaacagtt ggagtatctc ctaaatcacc
    16801 ttgaacgaaa cctcaattta gatgatcttg aagaattcac aattgaggca aatcctggtg
    16861 atttgacgcc agaaaaaatt gcggtgcttc agaggtcagc tgtcaatcgg atttccttgg
    16921 gggtgcagac ttttaataac aagcaattaa agcagattgg ccgtagccat aatgaagaac
    16981 aaatttatag caccattgcc aatttaaaaa ctgcaggctt tcacaatatt tccattgatt
    17041 taatttatgc cttgccaggt caaaccttag atcaagtcaa ggaaaatgtt gctaaagctc
    17101 tggcacttga tattcctcac cttagtcttt atagccttat tttggaacat cacactgttt
    17161 ttatgaacaa aatgcgacgt ggtaaactta acttaccaac ggaagacttg gaagcggaaa
    17221 tgtttgagta cattatttca gaaatggaag ctaatggttt tgagcattat gaaatttcca
    17281 attttaccaa accaggtttt gaaagtcgcc ataacttgat gtattgggat aatgtggagt
    17341 attttggatg tggtgcaggg gcttcaggtt accttaatgg cattcgttat cagaatcggg
    17401 tgcccatcca gcattatctt aaagcggttg aagcagggaa tgctagacta aatgaagaag
    17461 tgctgcgcaa agaggaaatg atggaagagg aactcttttt gggcttacgt aaaaagacag
    17521 gagtctccat ccagcggttc caagaaaaat ttggtgtttc ttttgaggag cgttatggaa
    17581 atattgttag ggagcttcaa aaccaaggac tgcttgtcaa agatgacgct tttgtcagaa
    17641 tgactaaaaa gggtcttttc ttaggtgatt ctgttgctga gcggtttata ttagattagg
    17701 agagtattat gggattaagt tatcaggagg agttgacact tccttttgaa ttatgtgatg
    17761 tcaaatcaga tataaaattg ccccttttat tagactattg tttgatggtt tctggtagac
    17821 agtctgcgca attaggacga agcaacaaca accttttagt cgattacaag cttgtttgga
    17881 ttgtaacgga ttatgagatc actattcatc gcttgccaca ttttcaagaa accatcacca
    17941 ttgaaacaaa agccctctcc tacaataaat ttttttgtta tcgccaattt tatatttatg
    18001 atcaagaggg gggtctttta gtggatatct tagcctattt tgctttgtta aacccagata
    18061 cgcgaaaagt ggcaactatt ccagaagatt tagtagcgcc ttttaagact gattttgtta
    18121 aaaagccaca ccgtgttcct aaaatgcctc ttttagaaca atcaattgat cgtgattatt
    18181 atgtgcgtta ttttgatatt gatatgaatg gtcatgtcaa caacagtaaa tatttagatt
    18241 ggatgtatga tgtgttgggg tgtgagtttt taaaaacgca tcagcctctt aagatgactt
    18301 tgaaatatgt taaagaagtc tcaccaggcg gtcaaattac ttccagttac catttggatc
    18361 aattaacttc ttaccatcaa atcacctcag atgggcagct gaatgcccaa gccatgattg
    18421 aatggcgagc gattaaacaa acagaaagcg agacagacta gtgccttata agggttattt
    18481 aattgattta gacggaacga tttaccaagg gaaaaaccgt attccagcag gggagcggtt
    18541 tatcaaacgc ctacaagaaa gaggcattcc ctatttatta gtgactaata ataccacaag
    18601 aacacccgaa atggtgcaaa gcatgctggc taatcagttt catgttgaga caggcattga
    18661 gaccatttat acggctacca tggcaaccgt cgattacatg aatgacatga accgtggcaa
    18721 gacagcttac gttattggag aaacagggct caaatcagct attgcagcag caggttacgt
    18781 agaagaactt gagaatcccg cttacgtggt ggttggttta gacagtcagg tgacctatga
    18841 gatgttggct atagccacct tggctattca aaaaggtgcc ctctttattg ggacaaatcc
    18901 tgatttaaac attccaaccg aacgtggttt gatgccagga gcaggtgcat tgaatgcctt
    18961 actagaagct gccacacgtg taaaacctgt ttttattggc aagcccaacg ctatcattat
    19021 gaataaatca ctagaggtat tgggcattca gcgctctgag gctgtcatgg ttggcgataa
    19081 ttacttgact gatatcatgg caggtattca aaatgatatt gcaacgatcc tcgtgacaac
    19141 aggctttacc agacctgaag aggtaccaac cttaccaatt cagccagatc atgtcctatc
    19201 tagccttgat gagtggaggt tgtgatggta gaaaacacta aattactatg tagctgggta
    19261 tggttattag cgttagctat tctcataacg atctattcaa cgtggctgtg gtatcctctt
    19321 gaggtggacc atctaaagtt agaacaagtt gtttttatga gcaaggatgc tatcttgcat
    19381 aactataacg gactactaaa ttacctcacc aatccttttg tgacaaggtt ggaatttgct
    19441 aattttcact catcagcaga cggactcaag cattttgcag atgtgaagtg gctttttcac
    19501 ctaactcaag tcgtttttct tggcctcctt tacccaaccc ttaagacttt cacacaaagg
    19561 ttaaaaacca aacgtttctg gctccttcaa aaacctttaa tactagcagc tttgtttcct
    19621 cttatgattg gtctaatggc tagtttcatt gggtttgaac atttttttac tctttttcat
    19681 caggtgcttt ttgtaggaga tagcagctgg ctctttgatc ccttgaaaga tcctgtgatt
    19741 tggatccttc cagaagtctt tttcttgcac tgtttccttt tctttatgat agtttacgaa
    19801 atcatcctgt ggagcttagt aggtttagca agatggcagc gcctaaatgt agaatgataa
    19861 aacaagcgtt ttgaaagcgc ttgtttttta gtttgcagtc agagtagaat agaagtatca
    19921 aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
    19981 cttgctatgc tgttttgaat ggttccaaca agattatttt ataactttta taacaaataa
    20041 tcaaggagaa attcaaagaa atttatc
//
LOCUS       DATUYR010000031        19233 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.031, whole genome shotgun sequence.
ACCESSION   DATUYR010000031 DATUYR010000000
VERSION     DATUYR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19233
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.031"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..330
                     /locus_tag="VCI33_001051"
     CDS             <1..330
                     /locus_tag="VCI33_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEP2831981.1"
                     /translation="TIDKDGQLSQENRYFIFSFKPDVLTFANCVRGHWQIESMHWLLD
                     VVYHEDHHQTLDKRAAFNLNFIRKMCLYFLKVMVFPKKDLSYRRKQRYISVHLEDYLV
                     QLFGERG"
     gene            499..2331
                     /gene="lepA"
                     /locus_tag="VCI33_001052"
     CDS             499..2331
                     /gene="lepA"
                     /locus_tag="VCI33_001052"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEP2831982.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDAKK"
     gene            2615..3748
                     /locus_tag="VCI33_001053"
     CDS             2615..3748
                     /locus_tag="VCI33_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP2831983.1"
                     /translation="MLTFGGASAVKADEQEEKAKVRTELIQELAQGLGGIEKKNFPTL
                     GDEDLDHTYMTKLLTYLQEREQAENSWRKRLLKGIQDHALDGQDGRNGERGEQGPTGL
                     AGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEP
                     GTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDG
                     QNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGK
                     PAPKTPEVPQKPDTAPHTPKTPQIPGQSKDVTPAPQNPSNRGLNKPQTQGGNQLAKTP
                     AAHDTHRQLPATGETTNPFFTAAAVAIMTTAGVVAVAKRQENN"
     gene            3934..4371
                     /gene="msrB"
                     /locus_tag="VCI33_001054"
     CDS             3934..4371
                     /gene="msrB"
                     /locus_tag="VCI33_001054"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP2831984.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDDFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYEQMEKEGYAQWLTLFDET"
     gene            4501..5520
                     /locus_tag="VCI33_001055"
     CDS             4501..5520
                     /locus_tag="VCI33_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEP2831985.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWYLGRLFPIIGAPVFAILLGMLL
                     ALFYHHRDKAKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNDGFATFAGTAVNDTSSVTATATAWDALHHSNTLDDATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSFGVS
                     ADVFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            5727..6152
                     /locus_tag="VCI33_001056"
     CDS             5727..6152
                     /locus_tag="VCI33_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2831986.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDNFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDDIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            6171..6662
                     /locus_tag="VCI33_001057"
     CDS             6171..6662
                     /locus_tag="VCI33_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP2831987.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            6679..7488
                     /locus_tag="VCI33_001058"
     CDS             6679..7488
                     /locus_tag="VCI33_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP2831988.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            7485..8312
                     /locus_tag="VCI33_001059"
     CDS             7485..8312
                     /locus_tag="VCI33_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP2831989.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            8448..10097
                     /locus_tag="VCI33_001060"
     CDS             8448..10097
                     /locus_tag="VCI33_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2831990.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQDLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            10101..10889
                     /locus_tag="VCI33_001061"
     CDS             10101..10889
                     /locus_tag="VCI33_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2831991.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            10883..11929
                     /locus_tag="VCI33_001062"
     CDS             10883..11929
                     /locus_tag="VCI33_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP2831992.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            12042..13439
                     /locus_tag="VCI33_001063"
     CDS             12042..13439
                     /locus_tag="VCI33_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEP2831993.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQDLADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGVKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            13541..15337
                     /gene="uvrC"
                     /locus_tag="VCI33_001064"
     CDS             13541..15337
                     /gene="uvrC"
                     /locus_tag="VCI33_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEP2831994.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLKIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            15522..16124
                     /locus_tag="VCI33_001065"
     CDS             15522..16124
                     /locus_tag="VCI33_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEP2831995.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            16249..17658
                     /gene="pepV"
                     /locus_tag="VCI33_001066"
     CDS             16249..17658
                     /gene="pepV"
                     /locus_tag="VCI33_001066"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEP2831996.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDRSADDQHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEYGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGINGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFNFDSQSADNTIALNFRYPKGTDAATLKSGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(17726..19102)
                     /gene="mnmE"
                     /locus_tag="VCI33_001067"
     CDS             complement(17726..19102)
                     /gene="mnmE"
                     /locus_tag="VCI33_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEP2831997.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQGSNRI
                     ILLNKTDLEQKIELEQLPADLISISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
BASE COUNT         5898 a         3526 c         4113 g         5696 t
ORIGIN      
        1 acgattgata aggatggtca gctgagtcaa gagaatcgtt attttatctt tagctttaag
       61 ccggatgtcc tcacatttgc caattgtgta cgaggtcatt ggcagataga gagtatgcac
      121 tggttattgg acgttgttta tcatgaagat catcatcaga cattggataa aagagccgca
      181 tttaacctaa attttatccg aaaaatgtgc ttatattttc tcaaagtgat ggtatttcct
      241 aaaaaagacc tcagttatcg tcgcaaacaa cggtatattt ctgtccattt ggaagattat
      301 ttagtccaat tatttggaga aagaggctaa gcagatgatt gatttaaaaa ggaatctttt
      361 caaaattgat gtccgggcac aaaaggatga agagaaagtt ttcatgcgta cggcgtggat
      421 aatccgtaag aaaagatagg caaaggccta ccttttttgt tataatagaa ggaaagacta
      481 aatgtatagg aaaatagaat gaacagtcaa gatttaaaaa aacgtcagga gaagattcgt
      541 aatttctcca ttattgcaca tattgaccat ggcaaatcta ctctcgctga ccgtattttg
      601 gaaaagacag agacggtttc gtctcgtgaa atgcaggctc agttattgga ctccatggat
      661 ttggagcgtg agcgtgggat taccatcaag ttaaacgcca ttgagcttaa ttacacggct
      721 aaagatgggg aaacctacat tttccacctt attgataccc cagggcatgt ggactttact
      781 tatgaagtat cgcgttcgtt ggcagcctgt gagggagcga ttctagttgt ggatgcagcg
      841 cagggcattg aagcacagac ccttgccaat gtttacctag cccttgataa tgatttggaa
      901 attttaccgg ttatcaataa aattgacttg cctgctgctg accctgagag agtccgccat
      961 gaagtagaag atgtcattgg acttgatgct tctgaggctg ttctggcgtc agccaaggct
     1021 ggtattggga tcgaagagat tcttgagcaa attgttgaga aggttcctgc tcctacgggt
     1081 gatgtggatg cgcctttaca ggccttgatt tttgactctg tgtatgatgc ttaccgaggg
     1141 gtaattttgc aggttcggat tgtgaacggt attgtcaagc ctggcgataa aatccagatg
     1201 atgtcgaatg gaaaaacctt tgatgttaca gaagttggga ttttcactcc taaggcagtt
     1261 ggacgagatt tccttgcgac gggagatgtt ggttatgttg cggcctctat caaaacggta
     1321 gcagataccc gtgtgggcga tacggtgact ttagctaata accctgctaa ggaagccttg
     1381 catggctaca agcagatgaa cccgatggtc tttgcaggga tttatccgat tgaatcaaat
     1441 aaatacaatg acttgcgtga agcgcttgaa aaattacaac tgaacgatgc cagtttacag
     1501 tttgaacccg aaacgtcaca agcgcttggc tttggttttc gatgcggttt tttaggcttg
     1561 cttcacatgg atgtgattca agaacgtttg gaacgtgaat ttaacattga tttgatcatg
     1621 acagcaccgt ccgtggtgta tcacgttcac acaacagatg aggacatgat tgaagtgtct
     1681 aacccttcag aatttccaga tccaacacgg gtggctttta ttgaagagcc ttatgttaaa
     1741 gcgcaaatca tggtgccaca agagttcgta ggggctgtta tggaattatc gcagcgcaag
     1801 cgtggtgatt ttgtgaccat ggattacatt gatgataacc gtgtcaatgt gatctaccaa
     1861 atcccgctgg ctgaaattgt ctttgatttc tttgataaat tgaaatcctc aacgcgaggc
     1921 tatgcgagtt ttgattatga catgtcagaa taccgcaggt cacaattggt gaaaatggac
     1981 attcttttga acggtgataa ggtcgatgcg ctcagcttta ttgttcataa agaatttgct
     2041 tatgaacgcg ggaaaattat tgttgagaaa ttgaaaaaaa tcattccacg tcaacagttt
     2101 gaagtgccta ttcaagccgc tattggtcaa aaaattgttg cgcgttcaga catcaaggcc
     2161 cttcgtaaaa acgttttagc gaagtgttat ggtggtgacg tgtctcgtaa acgtaaattg
     2221 ctcgaaaaac aaaaggctgg taaaaaacgg atgaaggcta ttggttctgt tgaagtccct
     2281 caagaagcct tcttgagtgt tctttcaatg gatgacgatg ccaagaaata accaagccta
     2341 atcgcttagt cttcagagga gaactcttgt gggtctacat gaccaaaaaa tggttatctt
     2401 atacaaaaag aactttacaa tcatttgcac ataatgttat aataatatta tagtgttatc
     2461 tcattattag gtgcgcctgc atttaatctt attaaacaga aagaggaaac aaaaactgtg
     2521 aacaaaacaa aaacaaaaca aaacaaaaca aaacaaaaca aaaacaaaac atagccttct
     2581 ttgtcgctat ggcttaacct cagcggctgc tcttttgctg acctttggag gtgcaagtgc
     2641 agttaaggcg gatgaacaag aagagaaagc taaagttaga actgaattaa ttcaagagtt
     2701 agctcaggga ctagggggta ttgagaaaaa aaattttcca actctaggtg atgaagattt
     2761 agatcatact tatatgacaa agctattaac atacctacag gaacgagaac aagctgagaa
     2821 tagttggcga aaaagactac taaagggtat acaagatcat gcccttgatg gacaagacgg
     2881 tcgaaacggt gaaagagggg aacaaggacc aacaggtctc gctggtaaag ccggtgaagc
     2941 tggagccaaa ggcgaaaccg gccccgctgg tccacagggt ccacgtggtg aacaaggccc
     3001 gcaaggtctt ccaggtaaag atggtgaagc tggtgctcaa ggcccagcag gtccaatggg
     3061 tcctgctggt gagcgaggtg aaaaaggaga acctggtacc caaggcgcta aaggtgatcg
     3121 cggtgaaacc ggtccagtag gtccacgtgg tgagcgaggc gaagccggtc ccgctggaaa
     3181 agatggtgaa cgtggtccag taggtccagc tggtaaggac ggccaaaacg gccaagatgg
     3241 tcttccaggt aaagacggta aggacggcca aaacggtaaa gatggtcttc caggtaaaga
     3301 cggtaaggac ggccaaaacg gtaaagatgg tcttccaggt aaagacggta aggacggtca
     3361 agatggtaaa gacggcctcc caggtaaaga cggtaaagat ggcctcccag gtaaggacgg
     3421 taaggacggt caaccaggta aaccggctcc taaaacacca gaggtccctc aaaaaccaga
     3481 taccgcacca catacaccaa aaacccctca gatccctggc caatcaaaag acgtgacacc
     3541 tgcccctcaa aacccttcta atagaggtct aaacaaacca caaacacaag gtggtaatca
     3601 gctcgcaaaa acaccagcag ctcacgacac acacagacaa ttgccagcaa caggcgaaac
     3661 aaccaatcca ttctttacag cagctgctgt agctatcatg acgacagctg gagttgtagc
     3721 tgttgcaaaa cgtcaagaaa acaactaatc ctctttttgc tacctaactg atggaaagta
     3781 aaactcagag agaacctcaa ggttctctct tttttgaatc aagatagtag ctctaggtat
     3841 aacgtcttgt ctgaggtgct gtgaacaaat cagttaccac tcacctcttt ttttggtaaa
     3901 atgtaagcac taagacatca aaaggagttt tccatggaaa catcagatga gttaaaacaa
     3961 cgcattggtg atctttctta cgaggtcacc cagcatgcag caacagaatc tccctttaca
     4021 ggcgaatatg atgatttttt tgagaagggt atctatgtcg atattgttag tggtgaggtc
     4081 ttattttcat cactggataa atttaattct ggctgtggtt ggcctgcctt ttcaaaaccc
     4141 attgaaaatc gtatggtcac aaaccacgat gactcttctt atgggatgag acgagttgag
     4201 gttaaaagcc gtgaagcagg atcgcatttg gggcatgtct ttagtgacgg ccccaaagaa
     4261 gctggaggct tgcgttactg tatcaattca gcagctctta aattcattcc ttatgagcag
     4321 atggaaaaag aaggctatgc ccagtggctc acattgtttg atgagacata accgggtttc
     4381 aaaatgactg aaaaagaagc gtcagtgatt cccacaagca ctggattggt gaggcgtacg
     4441 ggtcaatttt acaagccttt gtaaaaggtc tataataggg aatgaaaaaa ggaggttagt
     4501 atgtcaactc acttaagaaa acttccaggg ctgttacttt gcttgttgtt agctcttcca
     4561 gcctggtatt tagggcgctt atttcccatt attggagcac ctgtttttgc tattctttta
     4621 ggaatgttgt tagccttgtt ttatcaccat cgtgacaagg ctaaagaggg aattagtttt
     4681 acatccaagt atattttaca aacagcagtt gttttgcttg gttttggatt aaacctaacc
     4741 caagttatgg cagtgggcat gcagtcttta ccgattatca tttcaactat tgcgacagct
     4801 cttttggtag cttatggctc acagaaatgg ctgcgcttag atgtcaatac agccaccttg
     4861 gtgggtgtag ggtcttccat ttgtgggggg tctgctattg cagcgacggc tcctgtcatt
     4921 aaggcaaagg atgatgaggt tgctaaggca atttcagtca tttttctctt taatatgtta
     4981 gcagctttgc tatttccaag tttaggacaa ttactgggct tatctaatga tggttttgct
     5041 acttttgctg ggacagctgt taacgacacc tcttccgtga ctgcaacggc cacggcctgg
     5101 gatgcccttc accattccaa tacactagat gacgcaacca ttgtcaaatt gactcgcacc
     5161 ttggctattc ttccaattac tttaggttta tccctttacc gagcgaaaaa agagcacgac
     5221 atcgttacag aagaaaactt tagccttagg aagtcattcc ctcgtttcat cctcttcttt
     5281 ttattagctt ccctcatcac gacattgatg accagctttg gtgtttctgc cgatgttttc
     5341 cattccctaa aaaccttatc aaaattcttt atcgtgatgg ctatggcggc tatcggttta
     5401 aatacaaacc tggttaaatt aatcaagacg ggcggtcagg ctatcctttt aggagctatt
     5461 tgctgggtag ctatcaccct tgtcagttta gccatgcaat taagtttggg catttggtaa
     5521 tcagacacga gaaatcagtt tgactgattt ctttttttgt atcttatttg tgtagaattg
     5581 tctacatttt aaagagaata gtgcatttta aaaacgtttt catttgataa aataaaccta
     5641 tcgttaaacc agttcagtaa agacaactgg ttgagataag caattaccgt ttattcagcg
     5701 gtttttgtta atagaggagg ataggaatga aacgaaaatt tctcattggg agtcacggca
     5761 gactagccag tggtttgcaa agctctattg acattttagc aggtatggga caggcacttg
     5821 aaaccattga tgcttatgtt gatgatagtg actacactag ccagattgat aactttatcg
     5881 ctggggtagc agcagatgag cagggcctta tttttacaga tttgttaggc ggcagtgtga
     5941 atcagaaaat ggtaaccgct gtaatgaaca gtggcaaaga caatatcttt ttaatcacaa
     6001 acagtaactt agccacttta ttgtcgttgg tgtttttaaa accaggcgaa gcgcttacta
     6061 aagatgatat tgtaaccgtt atcaatgaat cgcaagttca gttagtggat ttggtccctg
     6121 agacaaattc agaagatgac ttttttgatt aatatctagg aggaagacaa atgattactc
     6181 aaattcgtgt tgacgaccgt ttgattcacg ggcaagtggc agttgtatgg accaaagagt
     6241 taaatgcgcc acttttagta gtggctaatg atgaagctgc aaagaatgaa atcacacaaa
     6301 tgacgcttaa aatggctgtg ccaaacggca tgaaactgct tattcgttca gttgaagact
     6361 cgattaagct ctttaatgat ccacgcgcca aagacaagcg tatttttgtg attgtcaatt
     6421 ctgttaaaga tgcttgtgcc attgctaaag aagttccaga cttagaagct gttaatgtgg
     6481 ctaacgtggg gcgttttgac aaatcagacc cagcaagcaa agtcaaagtg acaccaagtc
     6541 ttttacttaa cccagaagaa atggcagccg caaaggaatt agtcagttta ccagaacttg
     6601 atgtctttaa ccaagtctta ccatcaaata ccaaggtcca tttgagccaa ttagtcaact
     6661 aaagagaggt aaaaaagtat gttagtacca gcaacaatgg cagctttagc tgtattgatt
     6721 tgttttgggg gaaattattt aaccggtcaa agcatgatgg aaagacccct agtagttgga
     6781 ctggtcacag gtcttctttt aggagatatg aaggtcggta tcttaatggg ggcctctcta
     6841 gaagctcttt tcctaggaaa tgtgaatatt gggggtgtta ttgcagctga gcctgtaaca
     6901 gcgactgcca tggcaacaac ctttaccatt atttcacata ttgaccaaaa agcagccatg
     6961 accctagctg ttccaattgg aatgttggca gcctttgtgg ttatgttttt gaaaaacgtc
     7021 ttcatgaata tctttgctcc aatggttgat aaagctgcag cagctaacca tcaaggtaaa
     7081 ctagtgatgc tccactatgg cacttggatt atctattact tgatcattgc ctctatttct
     7141 ttcattggta ttttagtagg tagtggacct gttaatgcct ttgtggaaca cattcctcaa
     7201 aatctcatga atggcttgag tgctgcggga ggccttcttc cagctgtcgg ttttgccatg
     7261 ctgatgaaat tattgtggac caacaaatta gctgtttttt acctattggg ctttgtctta
     7321 acagcttatt tgaaattacc agcggttgca gttgctgccc taggagcagt tatttgcgtc
     7381 attagttccc aacgtgatct agaattagac gcaattacta gaggtgcaat tagtaaacaa
     7441 acaacctttg actctaaaga atcagaagag gaggacttct tcgcatgaca tcacaagata
     7501 atttgactaa agaagaccgt aaaatgcttc gctcagtctt ttggcgctca tggacgatga
     7561 atgctagtcg tactggggca acacaatacc acgcagtagg ggttatttac actttactac
     7621 cagtcatcaa tcgtttttat aagactgata aggataaagc agaggcactg gtccgtcata
     7681 caacatggtt caatgccacc atgcacatca ataactttat catgggtctt gtggcatcga
     7741 tggaaaagaa aaacagtgaa gatcctgatt ttgatgctag tgctatcacc gctgttaagg
     7801 cttcgttgat gggacctata tctggagttg gagattcctt cttctgggga attttaaggg
     7861 ttattgcggc aggtatcggg atttccttgg caagtgcagg ctcagccatg ggagctgttg
     7921 tcttcttact cttgtacaat attcctgctt ttattattca ctactatagc ctttatggtg
     7981 gttattctgt aggtgcagga tttatcaaaa aactttacga atctggtggt ataaaaatcg
     8041 tcactaaaac ctcaagcatg cttgggttaa tgatggtagg ctcaatgacg gcttcaaatg
     8101 taaaatttaa aacgattctt acagttgctg cgaaaggtgc caaagaagca gcttctattc
     8161 aagattacct tgaccaattg tttattggta ttgtaccatt aatggtaaca ctagcagctt
     8221 tttggcttct acgaaagaaa gtgaatatca actggattat gtttggtatc atgttccttg
     8281 gcattatcct tggactttta ggtatttgct aataatctcc tagcaataaa ggtaagtaag
     8341 acaagttttc ttgttttgct tacctttttc cttttaagat gatgagactt gttttgatga
     8401 aaaacgtagt agcattaaga aatttgttat aatgaaagga gcttaagatg cgaggtgaac
     8461 aggtggaaga acattttaaa aaacaattac aagatgatat ttctagacat ttttcgtacc
     8521 aatcgttaat gttatcattg ttgctaattg gtctttttat tattttttca ttagcgccac
     8581 aacaattagg cctctatcgt gatatcaatg ccactgcaac ccgttatcac cgtttgatta
     8641 gcaaacaaga agccttgctt gatgacttag ggaaaaatag cttactacct tttttaaata
     8701 aaaacctcag cactgctgat ttaagcaagc actattttca tttgcgccat agcagccaaa
     8761 cgtcaccaga acttttacta ttttcaccta gtcaagacct tttatttgct agtaacccac
     8821 atttaggaaa tgtttttagt aaatctgttt atattcaaga agtcttgaga gcagctcatc
     8881 ctccaaaaac cttgtttaaa gttgctatgg atagtgaaga tggtcactac ttgatgatca
     8941 ttaagccaat gattgatcaa aaccaactaa agggatatgc ttttttagtg atgagtggca
     9001 aagattttct tcatcctaca aaaacactga cgtcagagtt ggtcattgct gataagctgg
     9061 acaatacgtt tacgttttcc aatcgtgagt ttatagcatc tagtctagat aagatcaaca
     9121 gtcagtattt acaccattat tttgtttttc aagataatcg agcctttatc acaagaaagg
     9181 ttgccttaca aggaggtctt tggctttaca tgtataggcc cttgattcca atggtatcgg
     9241 tgatgttatt ttcacttatt tcatcagctg ttatctttgt gatcttgcaa cgtaaatcaa
     9301 gtggtttagc taaccgaatt gcagctaaaa attcaagagc aatcaatcaa atggttagag
     9361 atatgagtgc gatctctcgg caagaaaaaa gacgtattga ccttgagagt caagatgaat
     9421 ttcaatattt atctgatcaa atcaatcaaa tggtagagcg attgcaacag ttacatgata
     9481 aaacgttaga tttggaaact caaaaattat tatttgaaaa acggatgtta gaagctcaat
     9541 tcaatccgca tttcctctac aatacgctag aaaccatttt gattacgagc cattatgatt
     9601 ctgccctaac agaaaaaatt gttatccaat tgacaaaatt gttgcggtat agtctcacgg
     9661 attctagcaa acctgtcttg ctcaaagatg atttaagtgt catcgagtct tatttagtga
     9721 ttaatcaggt gcggtttgaa gaattgcaat atagcattaa cctatcccca gaccttgata
     9781 gtcttgcagt gcctaaattg ttcttgttac ctttgataga aaatgccatc aaatatggct
     9841 tgaaagaacg acacgatgtc aagattaaca ttgcttgtta ttatcaagat gaccatatta
     9901 ttttttctgt gcgagataat ggttcgggaa ttgatgctca tcaccaaaaa gtgattcgag
     9961 agcaattgga agctggagag tcacaccatg ggttaattaa ctcttatcgt cgacttaagt
    10021 atcatttttc agaagtatcc ttggtttttg atcaaggtga taaacagttt aatgttagct
    10081 accatgttaa ggagtagtct atgtacaaat tagtcattat tgaagatgaa catattatta
    10141 ggaaatggtt gcgatacgct attgattata aagctttaga tattttggtc attggcgagg
    10201 ctaaagatgg caaagaagga gctgttttaa tcaaagagag tcaaccagat atcgttttaa
    10261 ctgatattaa catgcccatc atgacagctt ttgacatgtt tgaagcaaca aaagatcaaa
    10321 cttacgccaa aattatttta tctggctatg ccgattttcc aaatgcaaga tctgctatac
    10381 actacggtgt acttgaattt ttaacaaaac cgattgaaaa agcagctctt tgggaatgtc
    10441 ttcaaaccat tattgctaaa atagaaaagc aaaaggggtc taatcaaaaa acagatgctt
    10501 gcgtctatat tcccttaccc cagatgactg atcagattcc tgaagtagtc aaagatattt
    10561 tggagtgggt acatgctcat tttcaagaca agatcagcac gagccgccta gctcatgact
    10621 tagggtatag tgagagttat atctatcaaa atatcaaaaa gcatcttcag atgcctttaa
    10681 gtgattacat taatcaatat cggattaatc aggcaattca attgatgcaa caagaaccag
    10741 atttaatggt atatgaaata gcacaagctg taggtattta tgactatcgc tattttgata
    10801 gggtttttaa aaagtatcta ggacaaactg taaaggcttt taaagaagag cattttatga
    10861 aggataccga taggagtaag ccatgttaaa actcaaacgc aaatggctat taagcttttt
    10921 gttagtaata attattctag caatcgttaa cattgcaatg tatatattct catcgtcaaa
    10981 aaaagatagt gctaaagaat tagttatctt gactcctaat agccaaacta ttttaacagg
    11041 gactattcca gcctttgagg aaaagtatgg ggttaaagta agattaatcc aaggtgggac
    11101 gggccaactt attgatcaat taggtcgaaa agataagcca ttaaacgctg atattttctt
    11161 tggtggcaat tacactcaat ttgaaagcca taaagattta tttgaatcct atgtttctcc
    11221 gcaggtttct actgtcattt cagattacca attgcctagt catcgtgcaa ccccatatac
    11281 gatcaatggc agtgtactga ttgttaataa cgaattagca agaggacttc atattaccag
    11341 ttatgaggat ttgctacaac cagctttaaa aggcaaaatt gcttttgctg acccaaatag
    11401 ctcatctagc gctttttcac aattaacaaa catcttactg gctaaaggag gctatacgag
    11461 ctcagaagct tgggagtatg tgaaacgttt attactgtct atgaattcta tcagagcaac
    11521 aagttcttca gatgtttacg aatctgttgc agaaggtaaa atgattgttg gtttgaccta
    11581 tgaagaccct tgcatcaatc tgcaaaaaag cggtgcaaac gtttcgattg tttatccgga
    11641 tgaggggaca gtatttgttc cgtcatcagt cgctattatc aaaaaagcaa aggcaatgaa
    11701 agaagctaag caatttatta attttatttt atcatcagat gtacaaaatg cctttgggca
    11761 gtcaactagt aatagaccta tccgacgaga tgctcaaacg agtcatgaaa tgaaaccatt
    11821 agatgaaatt attactttaa aagaagatta cggatatgtt actagacata agaaaaaaat
    11881 attgaatacc tataatcgac tacatgaagc attagaaaga ggaaagtaga ctctaggctt
    11941 gttgttgcta tactagtagt gttacaaaat agcgtagtca ttttttgaaa tcgatttcat
    12001 aatctggtag actaagatta tcacgaaaag gagataatat aatggcttat caaactattt
    12061 acccttatac aaatgaagtc cttcatacat ttgataatat gaccgatcaa gatttggcag
    12121 atgtccttga aagagctcac cttctctata aaaaatggcg caaggaagat catttagaag
    12181 aacgtaaagc ccagcttcat caagtggcta atattcttcg ccgtgaccgc gataaatatg
    12241 ctgaaatcat gaccaaagac atgggaaaac tgttcacaga agcccaaggt gaagtagatc
    12301 tttgtgccga tattgctgat tactacgctg ataaggcaga tgaatttttg atgtctacgc
    12361 cactagaaac agattcaggt caagcttact atttgaaaca atcaactggt gttattctag
    12421 ctgttgaacc ttggaatttc ccttactatc aaatcatgcg ggtctttgcg ccaaacttta
    12481 ttgttggaaa cccaatggtt ttaaaacacg cttctatttg cccacgatct gcccaatcct
    12541 ttgaagaatt agttcttgaa gctggtgctg aagcaggtag tattaccaat ttgtttattt
    12601 cttatgacca agtatcacaa gtgattgctg ataaacgtgt ggttggtgtc tgcttgacag
    12661 gttccgaacg tggaggagct agcattgcag aagaagctgg taaaaacttg aaaaaaacaa
    12721 cccttgaatt gggtggcgat gatgccttta ttatccttga tgacgcggat tgggatcaat
    12781 tagagaaggt gctttacttc tctcgtctct acaatgcagg tcaagtatgt acctcatcga
    12841 aacgttttat cgtgcttgac aaagactatg accgtttcaa agagttatta acaaaagtct
    12901 tcaaaacagc taaatggggt gatccaatgg atcccgaaac gacgttggcg ccattatcat
    12961 cagctcaagc taaagcagat gttcttgacc aaattaagtt ggccttggat catggtgcgg
    13021 agttggttta tggtggtgaa gcgattgatc atccaggtca ttttgtcatg ccaaccatta
    13081 ttgctggcct cacaaaagac aaccctattt actatcaaga aatctttggc ccagtcggtg
    13141 aaatctacaa agtgtcttct gaagaagaag cgattgaagt ggcgaatgat tctaactatg
    13201 gccttggagg tactatcttt agtagcaatc aagaacatgc gaaagccgta gctgctaaaa
    13261 ttgaaactgg tatgtctttt atcaactcag gctggacaag tcttccggaa ctcccatttg
    13321 gtggggttaa acattcaggc tatggccgtg aattgagtga gcttggcttt acatcttttg
    13381 tgaatgaaca ccttatctat attccgaata aaacgaataa tagcaatact aaagtttgat
    13441 ctagattgtt aaagaagagt gtaacgcctt aataaacaat caaggcgtta cactcttctt
    13501 ttttaaagtc aaaattttaa aaatttgata aactagtaac atgaacgaac tgattaaaca
    13561 taaactagaa ctcttaccag acagccctgg ctgttactta cataaagata aagaaggaac
    13621 tattatctat gtcggtaagg ctaagaattt gaaaaaacgc gtccggtcct attttcgtgg
    13681 gagccatgat accaaaacag agttactagt atctgagatt gtagatttcg aatatattgt
    13741 aacagagtca gataccgaag cgcttttgtt ggaaattaac ttgattcaaa aaaatatgcc
    13801 taagtacaac atcaagttaa aagatgataa atcttatccc tttctcaaaa tcacaaatga
    13861 gtcttttcct cggttagtga ttacaagata tattaagaaa aatgatggat tgtactttgg
    13921 gccctaccca gattcttaca ctgctaatga agttaaaaag ttattagata ggatttttcc
    13981 atttaaaaag tgtaaaaatc ctatcaataa ggtctgtttt tattaccatt taggccagtg
    14041 ctgtgctcat accatttgtc atacagacaa ggcctattgg gatcgtttaa ttgatgatgt
    14101 taagcacttt ttaaatggta aagatgacaa aattattgaa gatcttcgtt ctaagatgtt
    14161 agcagcttct gaagaaatgg catttgaacg tgccgctgaa tatcgagatt tgatttcagg
    14221 tattgccacc atgcgcacca agcagcgggt gatgagcaag gatttgcaag atagagatat
    14281 ctttggctat tatgttgata agggatggat gtgtgtccaa gttttctttg tgcgtcaagg
    14341 gaaattaatt cagcgtgatg ttaacctttt tccctattat aacgatgcag aagaagattt
    14401 cttaacttat atggggcaat tttaccaaga taaacagcat ttcatcccta aagaagtctt
    14461 tattccagaa gctattgatg aagaactggt agccgctatt gtgccaacca aaatcatcaa
    14521 acccaaacgc ggtgaaaaaa aacaactggt agctttggct actaagaatg ctcgtgttag
    14581 tctgcaacaa aaatttgatt tattagaaaa agatatcaaa aaaacgagtg gtgctatcga
    14641 aaatttaggt cagttactta agattgacaa gcctgtgcgc attgaagcct ttgataattc
    14701 taacattcaa gggacaagtc cagttgcggc tatggttgtt tttgttgatg gcaaacctag
    14761 taagaaagat tatcgtaaat ttaagattaa aacagtggtg ggaccagatg attatgccag
    14821 tatgagagaa gtgctttttc gaaggtacag tcgggtgaaa aaggagggac tgcaagctcc
    14881 taacttgatt attgttgatg gaggcgttgg acaagtcaat gtggccaaag atgtgataga
    14941 aaaacaactt ggactcacta ttcctgttgc gggacttcaa aaaaacgata agcaccagac
    15001 ccatgatttg ctttttggaa acccacttga agttgtaccg ttgccgcgcc gttctgaaga
    15061 gtttttctta ctgcatcgga ttcaagacga agtgcatcgc tttgcagtta catttcatag
    15121 acaagtgcga cgtaaaaatt ctttttcatc aacattagat catatttcag ggcttggccc
    15181 aaagcgaaaa cagttattac taagacactt taaaaccata acagcgattg catcagctac
    15241 ctctgaggag attcaagctt taggtatacc taaaacagta gttgaagcaa tacagcaaca
    15301 gataacagac aataaaaatg atagatcgtc tccttaaata agtgttatgt tttcagtact
    15361 gatgaggtaa aaaggcgcta cttgttacgc acgacatttc agttcgttat tttgaaactt
    15421 tcagaaagtt gcgaagtgta gtgagtttat gattttaaaa actattttat ggtaaaataa
    15481 aatggtatta aaaaaaacag atttagatag gaagttttga aatgaaattt ctagagttaa
    15541 acaaaaaacg ccatgccatt aagaccttta atgaccagcc agttgactac gaggatttgc
    15601 gaacagccat tgagattgca accttagcac ctagtgccaa taacattcaa ccttggaaat
    15661 ttgtagtcgt tcaagagaaa aaagctgagt tagcaaaagg gttacctcta gcaaataaag
    15721 tgcaggttga acaggctcaa tatgtagtgg cgctattttc agacactgat ttagctttac
    15781 gttctcgtaa aattgcccgt atcggtgtca aatcgttacc agacgactta attggttatt
    15841 atatggagac tttgccacca cgttttgctg cctttaatga ggttcaaacc ggtgagtact
    15901 tagctattaa tgcaggcatt gtggctatga acttggtgtt atccttgacg gatcaaaaga
    15961 ttgcttccaa tattatcctt ggttttgata agtcaacgac caatgaaatt ttagacatcg
    16021 atccgcgttt tcgtccagaa ttattgatta ctgttggcta tagtgacgag aaaccagaac
    16081 caagttatcg tttgcctgtt gatgaagtca tcgaaagaag atagagtcag gacaattagt
    16141 taaatgtaat gtccagatgc gctacggagc gaccgctatt gttagactga tactattttg
    16201 gcttatttta agttaaacat tatccctaaa acgaataagg agagtataat gacaaccatt
    16261 gattttaaag cagaagttga caaacgtaaa gaagctatgc tagcagattt agttgaccta
    16321 ttacgcatta attcagaacg cgatgacaga tcggccgatg atcagcatcc ctttgggcca
    16381 ggtccagtta aggctttgga gcatttcctc gcaatggctg agcgtgatgg ctacaaaacg
    16441 cgtaatattg ataactatgc aggtgacttt gaatttggtc aaggtgatga agtactagga
    16501 atctttggcc accttgatgt tgtgccagct ggtagcggtt gggatacaga cccttatgag
    16561 ccagtgatta aagacgatcg catctatgcg cgtgggtctt cggatgataa gggaccaacc
    16621 atggcgtgct actacgctct gaaaatcatt aaagaattgg gtcttcctgt gtctaaaaaa
    16681 gttcgtttca ttgttggtac cgatgaagaa tctggctggg gggatatgga ttattacttt
    16741 gctcataatg gcttgaaaaa tccagatttt ggtttctcac cagatgctga atttccaatt
    16801 atcaatggtg agaaaggaaa tatcaccgaa tacctccatt tcgctggtga caacaagggc
    16861 gcttttgtgc ttcatcgctt ccaaggtggg ttgcgtgaaa acatggtacc agaatctgcc
    16921 acagctgtca ttacggcacc gcatgacctt gatgtgctag aagcagcctt ggagcaattt
    16981 ttatcagaat atggtgttaa aggctctatg aaggctactg atggtaaaat tgaagtaact
    17041 attattggga agtctgctca cgggtcaact ccagaagctg gaatcaatgg tgccaccttg
    17101 ctggctaaat tcctgaatca atttaccttt gaaggggctg ctaaagatta tttacatgtg
    17161 gcaggtgaag tgcttcatga agactttgcg gcagaaaaac taggccttgc ttataccgac
    17221 gaccgtatgg gagccttgag catgaatgca ggtgtcttta actttgatag tcaatcagct
    17281 gacaatacca tcgccttgaa tttccgttat ccaaaaggga ctgatgcggc aaccttaaaa
    17341 tcaggattgg aaaaattacc aggtttaact aaggtaagcc tttctgagca tgaacataca
    17401 cctcattatg tgccaatgga tgatgaattg gtagcaacct tgcttgctgt ttacgaaaaa
    17461 caaacaggtc ttaaaggcta cgaacaagtt atcggcggag gaacctttgg tcgtttgcta
    17521 gaacgtggtg tggcctttgg agcaatgttc ccaggtgatg aaaacactat gcaccaagct
    17581 aatgaatata tgcctttaga aaatatttat cgctcagcag ctatttatgc tgaagcaatc
    17641 tacgaattaa ttaaataaga tgtttatgac agcaaaaaga gctggaacag tgttccagct
    17701 cttttgtgat gattagagac tgttgttatt taccaagaca gaattgacta aagagttggg
    17761 taatgagctc atcaggagca gcatcccctg tgatttctcc gagaatttcc caagttctgg
    17821 ttaaatcaac ctgtagtaaa tcaactggca tgccaagcgc caagccgtca ttgacagctt
    17881 ctaaactctg gactgccttt tcaatcaaag aaatgtggcg ggcatttgac aggtaagtgg
    17941 catcttgttc tactagtcca gcattatcaa agaagagctg gttgatacga tcttcgatca
    18001 gattaatgtt ctggttggtt aacaccgaaa tagaaataag gtccgctggc aactgttcca
    18061 attcaatctt ttgttccaaa tctgttttat tcagcaatat aatgcggtta ctgccctgac
    18121 tgagatttaa caaagcccgg tcttggtcag tcagtttttc agaagcattc agcacaagaa
    18181 gaacaaggtc agcttcttga agagcttttt tagaccgttc aactccgatt tgttcgacca
    18241 gatcatctgt ctcacgaata ccggccgtat cgacaagttt tagaggaact cccttgatat
    18301 taacgtattc ttcaatgaca tctcgggtgg ttcctgcaat atcagtcaca atagccttgt
    18361 cttctcgcaa caaattatta agtaagcttg acttaccgac gtttggacga ccaataatag
    18421 ctgttgataa gccctcacgc agaattttac cgcgtttagc tgttcgaagg agattttcca
    18481 aaaggctttg gaattcttgt gttttttcac gtagcagagc agtggtcatt tcttccacat
    18541 cgtcgtattc agggtagtca atattaactt cgacttgagc taaggtgttg agaatttctt
    18601 gtcgggtgtc attgataagc tgggaaaggg agccatccag ttgcttgact gcaatcgtca
    18661 tagccttgtc tgttttggca cgaataatgt ccatgacagc ctcagcttga gtcaaatcaa
    18721 cacgaccatt taagaaggca cgtttggtga attcgccagg ctctgccatt ctagcgcctt
    18781 ggcgaatcaa tagttgcaag atttcatttg tcacagcaat accaccgtgc gtattgattt
    18841 caaccacatt ttcacgggta aaggttttgg gggctagcat gactgacacc ataacttcat
    18901 cgataatagt tcctgtctta ggatcaatga tatggccata gttaatggtg tgtgaggcga
    18961 cttgctctaa gttctttccc ttaaaaacag attgtgcaat ggcaagcgca tctgtcccgg
    19021 atagacgaac aatgccaata gctccttcac caagaggggt tgaaatggcg gtaattgtat
    19081 caaattcttt agttatactc atgccctcta ttttaaatca agtcctagta gaaagcaagg
    19141 tattattttg tatagaaaat aggactagca ccattttgag tttagctaat actaaaaaaa
    19201 taaatgtaaa aaaatcattt ttttaataat ttt
//
LOCUS       DATUYR010000032        18669 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.032, whole genome shotgun sequence.
ACCESSION   DATUYR010000032 DATUYR010000000
VERSION     DATUYR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18669
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.032"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            283..1641
                     /locus_tag="VCI33_001068"
     CDS             283..1641
                     /locus_tag="VCI33_001068"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEP2831998.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDEDFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            1641..2723
                     /locus_tag="VCI33_001069"
     CDS             1641..2723
                     /locus_tag="VCI33_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEP2831999.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            2724..3872
                     /locus_tag="VCI33_001070"
     CDS             2724..3872
                     /locus_tag="VCI33_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEP2832000.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQAHG"
     gene            4084..5448
                     /gene="ftsA"
                     /locus_tag="VCI33_001071"
     CDS             4084..5448
                     /gene="ftsA"
                     /locus_tag="VCI33_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEP2832001.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            5472..6791
                     /gene="ftsZ"
                     /locus_tag="VCI33_001072"
     CDS             5472..6791
                     /gene="ftsZ"
                     /locus_tag="VCI33_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEP2832002.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            6794..7465
                     /locus_tag="VCI33_001073"
     CDS             6794..7465
                     /locus_tag="VCI33_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEP2832003.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            7477..8133
                     /locus_tag="VCI33_001074"
     CDS             7477..8133
                     /locus_tag="VCI33_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEP2832004.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            8138..8392
                     /locus_tag="VCI33_001075"
     CDS             8138..8392
                     /locus_tag="VCI33_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEP2832005.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            8392..9183
                     /locus_tag="VCI33_001076"
     CDS             8392..9183
                     /locus_tag="VCI33_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEP2832006.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVGDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            9193..9951
                     /locus_tag="VCI33_001077"
     CDS             9193..9951
                     /locus_tag="VCI33_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEP2832007.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            10224..13025
                     /locus_tag="VCI33_001078"
     CDS             10224..13025
                     /locus_tag="VCI33_001078"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEP2832008.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRDDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMTENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNTKENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGIAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESDK"
     gene            complement(13290..13592)
                     /locus_tag="VCI33_001079"
     CDS             complement(13290..13592)
                     /locus_tag="VCI33_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEP2832009.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(13643..14098)
                     /locus_tag="VCI33_001080"
     CDS             complement(13643..14098)
                     /locus_tag="VCI33_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP2832010.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLSWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(14226..16508)
                     /locus_tag="VCI33_001081"
     CDS             complement(14226..16508)
                     /locus_tag="VCI33_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP2832011.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            16806..17036
                     /locus_tag="VCI33_001082"
     CDS             16806..17036
                     /locus_tag="VCI33_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEP2832012.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            17163..17849
                     /locus_tag="VCI33_001083"
     CDS             17163..17849
                     /locus_tag="VCI33_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP2832013.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSAFLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            17849..18583
                     /locus_tag="VCI33_001084"
     CDS             17849..18583
                     /locus_tag="VCI33_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2832014.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
BASE COUNT         5795 a         3250 c         3957 g         5667 t
ORIGIN      
        1 tttttaatag ttcatttttt ctgaaagaaa gcttttagcc gattttcatt gagtgtgcaa
       61 ctaaaaaaag atgcttggta gtgacagtta agacctctga ctagtatttt atcttacttt
      121 acttaaaaaa acgtaccaat aatctcacaa atatgggcat gatgtttgta tattggaaac
      181 ggattctaat tctaaactca agaacggtat ttttaaaatg ttgaaaaagc atctaaataa
      241 aggtaaaata gaaatcaaat agaatggata gtgtgagctt agatgaaagt gataagtaat
      301 tttcaaaata aaaaaatatt aatattgggg ttagccaaat cgggcgaagc agcagcaaaa
      361 ttattgacca aacttggtgc tttagtgact gttaatgata gtaaaccatt tgaccaaaat
      421 ccagcggcac aagccttgtt ggaagagggg attaaggtca tttgtggtag ccacccagta
      481 gaattattag atgaggactt tgagtacatg gttaaaaacc ctgggattcc ttatgataat
      541 cctatggtta aacgcgccct tgcaaaggaa attcccatct tgactgaagt agaattggct
      601 tatttcgtat ctgaagcgcc tattatcggg attacaggat caaacgggaa gacaaccaca
      661 acgacaatga ttgccgatgt tttgaatgct ggcgggcaat ctgcactctt atctggaaac
      721 attggttatc ctgcttcaaa agttgttcaa aaagcaattg ctggtgatac tttggtgatg
      781 gaattgtcct cttttcaatt agtgggagtg aatgcttttc gccctcatat tgctgtcatc
      841 actaatttaa tgccgactca cctggactat catggcagtt ttgaggatta tgttgctgct
      901 aaatggatga ttcaagctca gatgacagaa tcagactacc ttattttaaa tgctaatcaa
      961 gagatttcag caactctagc taagaccacc aaagcaacag tgattccttt ttcaactcaa
     1021 aaagtggttg atggagctta tctgaaggat ggaatactct attttaaaga acaagcgatt
     1081 atagctgcca ctgacttagg tgtcccaggt agccacaaca ttgaaaatgc cctagcaact
     1141 attgcagttg ccaagttatc tggtattgct gatgatatta ttgcccagtg cctttcacat
     1201 tttggaggcg ttaaacatcg tttgcaacgg gttggtcaaa tcaaagatat taccttctac
     1261 aatgacagta agtcaaccaa tattttagcc actcaaaagg ctttatcagg ttttgataac
     1321 agtcgcttga ttttgattgc tggcggtcta gatcgtggca atgaatttga cgatttggtg
     1381 ccagaccttt taggacttaa gcagatgatt attttgggag aatccgcaga gcgtatgaag
     1441 cgagctgcta acaaagcaga ggtctcttat cttgaagcta gaaatgtggc agaagcaaca
     1501 gagcttgctt ttaagctggc ccaaacaggc gatactatct tgcttagccc agccaatgct
     1561 agctgggata tgtatcctaa ttttgaggtt cgcggggatg aatttttggc aacctttgat
     1621 tgtttaagag gagatgccta atgcctaaga agattttatt tacaggtggt ggaactgtag
     1681 gtcatgtcac cttgaacctc attctcatac caaaatttat caaggacggt tgggaagtac
     1741 attatattgg tgataaaaat ggcattgaac atacagaaat tgaaaagtca ggccttgacg
     1801 tgacctttca tgctatcgcg acaggcaagc ttagacgcta tttttcatgg caaaatctag
     1861 ctgatgtttt taaggttgca cttggcctcc tacagtctct ctttattgtt gccaagcttc
     1921 gccctcaagc ccttttttcc aaaggtggtt ttgtctcagt accgccagtt gtggctgcta
     1981 aattgcttgg taaaccagtc tttattcatg aatcagatcg gtcaatggga ctagcaaaca
     2041 agattgccta caaatttgca actaccatgt ataccacttt tgagcaggaa gaccagttgt
     2101 ctaaagttaa acaccttgga gcggtgacaa aggttttcaa agatgccaac caaatgcctg
     2161 aatcaactca gttagaggcg gtgaaagagt attttagtag agacctaaaa accctcttgt
     2221 ttattggtgg ttcggcaggg gcgcatgtgt ttaatcagtt tattagtgat catccagaat
     2281 tgaagcaacg ttataatatc atcaatatta caggagaccc tcaccttaat gaattgagtt
     2341 ctcatctgta tcgagtagat tatgttaccg atctctacca acctttgatg gcgatggctg
     2401 accttgtagt gacaagaggg ggctctaata cactttttga gctactggca atggctaagc
     2461 tacacctcat cgttcctctt ggtaaagaag ctagccgtgg cgatcagtta gaaaatgcca
     2521 cttattttga gaagaggggc tacgctaaac aattacagga acctgattta actttgcata
     2581 attttgatca ggcaatggct gatttgtttg aacatcaggc tgattatgag gctactatgt
     2641 tggcaactaa ggagattcag tcaccggact tcttttatga ccttttgaga gctgatatta
     2701 gctccgcgat taaggagaag taaatggcaa aagataaaga gaaacaaagt gatgacaagc
     2761 tcgttttgac agagtggcaa aagcgtaaca ttgaattttt aaagaaaaag aagcagcaag
     2821 ctgaggaaga aaaaaaactc aaagagaaat tattgagtga taaaaaagcg cagcagcaag
     2881 ctcaaaatgc ttctgaagca gttgagctta aaactgatga gaaaactgat agtcaggaaa
     2941 ttgagtcaga aacgacgtca aaacctaaaa aacccaaaaa agttagacaa cccaaggaaa
     3001 aaagcgcgac acaaatcgct tttcaaaaat ccttgcctgt tcttttgggg gcgctcttac
     3061 ttatggcggt gtctattttt atgatcactc cttatagcaa aaagaaagag ttttctgtaa
     3121 gaggaaacca tcaaacgaac cttgacgaat taatcaaagc tagcaaagtc aaagcatctg
     3181 actattggtt aacgttgtta acttcgcctg gtcagtatga acgaccgatt cttcgtacta
     3241 ttccatgggt gaaatctgta catctctctt accaatttcc taatcacttt ctatttaacg
     3301 ttattgaatt tgaaatcatc gcttatgcac aagtcgaaaa cggttttcag cctattttgg
     3361 agaatggaaa acgtgtggac aaggtcaggg catcagaact accgaaatct ttcttgattc
     3421 ttaatttaaa agatgagaaa gcgatccaac agttagttaa gcaattaacg acattaccta
     3481 aaaaattagt caagaatatc aagtcagtgt ctcttgcaaa ttccaaaacg acagcggatt
     3541 tactacttat tgaaatgtat gacggtaatg tagttagagt accgcagtca caactcacat
     3601 tgaaacttcc ctattatcaa aaattgaaaa aaaaccttga aaatgatagt atagtggata
     3661 tggaagttgg aatttacact acaacacagg agattgaaaa tcaacctgaa gttcctctta
     3721 cgccagaaca aaacgcagct gataaagaag gagataagcc tggtgagcat caggaacaga
     3781 cagacaatga ttcagaaacg ccagcaaatc agagtagtcc tcagcaagca ccaccatccc
     3841 cagaaacggt cctcgaacag gcccatggct agctaatatc taagttgaaa aagcaatgaa
     3901 aacgttagaa attcaacgat tctaacccat aatgaattgc ctaaaaaaaa ttaagtttat
     3961 ataacaaaaa acgtaaaatg ataacatttt acgttttttt atggtataat attttctgaa
     4021 tgattctgtt ttttagcagt ttttagaata gcaaaagttt ggaaagtaag tgaggtcaag
     4081 tgaatggcta gaaatggctt ttttactggt ttggacattg gaacaagctc gataaaagtt
     4141 ttagtagcag aatttatttc tggtgagatg aacgtcattg gtgttagtaa tgttccaagt
     4201 accggcgtaa aagatggcat aataatcgat atagaggcag ctgcgactgc catcaaaact
     4261 gcggtagaac aagcagaaga aaaagcaggg atgacaattg aaaaggttaa tgttgggcta
     4321 ccggcaaacc ttcttcaaat tgaaccaaca caaggaatga ttcctgtccc aagtgagtct
     4381 aaagagataa aagatgagga tgttgatagc gttgttaaat cggctttaac aaaaagtatc
     4441 acaccagaac gagaggttat ctctttagtt ccagaagagt tcattgtgga tggctttcag
     4501 ggcattcgag atccacgtgg tatgatgggg attagattag agatgcgcgg gcttatttat
     4561 actggaccaa gcaccatttt acataatctg cgtaaaacgg tagaaagagc aggcattaaa
     4621 gttgaaaaca tcattatttc tccgttagct atggctaaaa ccattttaaa cgaaggtgag
     4681 cgcgagtttg gagctactgt aattgatatg ggaggtggac agacaactgt cgcttctatg
     4741 cgagcacaag aattgcagta taccaatata tatgctgaag gcggcgaata cattactaaa
     4801 gatatatcaa aagtattaaa aacgtctttg gctattgcag aagcacttaa gtttaatttt
     4861 ggtcaagcgg agatatcaga agctagtata actgaaacag taaaagttga tgtggtaggt
     4921 agtgaagagc ctgttgaggt aactgaacgt tatttatctg aaattatttc agcgcgtatt
     4981 cgtcatattt tagatcgtgt gaagcaagat ttggaaagag gtcgtttact agacttacca
     5041 ggaggcattg ttttgattgg tggcggtgca atcatgcctg gagtggtaga aattgcacaa
     5101 gaaatctttg gagtaactgt aaagctccat gttccaaatc aagtcggtat tagaaatcca
     5161 atgttttcaa acgttatcag tttggtagaa tatgttggta tgatgtctga agtagacgtt
     5221 ttagcacaaa ctgcagtttc aggagaagaa cttttgcgac gcaagcctat cgatttcagt
     5281 ggtcaagaat cttatttacc agattatgat gattcaagaa gaccagaatc gaccattggc
     5341 tatgaacaac aagcgtcaca aacagcatat gattcacaag ttccgagtga tcctaaacaa
     5401 aaaatttcag aacgtgttcg tggcatattt gggagtatgt ttgattaaaa gtaataaagt
     5461 gaggagataa aatggcgttt tcatttgata ctgcatcaat tcaaggtgca attataaaag
     5521 taattggagt cggcggaggt ggcggaaatg ccattaatcg tatgattgat gaaggtgttg
     5581 ctggtgtcga gttcatcgca gcaaatacag acattcaggc attaagctca tcaaaagctg
     5641 aaacggttat tcaactaggc cctaaattaa ctcgtggact tggtgctgga ggacaacctg
     5701 aagtaggacg taaagctgct gaagaaagcg aagaaatttt aacagaagct cttacaggag
     5761 cggatatggt atttattact gccggtatgg gtggtggctc tgggacaggg gctgcaccgg
     5821 ttattgctcg tatcgctaag agtttgggag ccttgacagt agctgttgtt actcgcccgt
     5881 ttggttttga aggtaacaaa cgtggtaatt ttgctattga aggtatcgaa gaactccgtg
     5941 aacaagttga tactttgtta attatttcaa ataataacct tcttgagatt gttgataaaa
     6001 agacaccttt attagaagca cttagtgaag ctgataatgt tttacgtcag ggagttcaag
     6061 ggataaccga cttaattact agtcctggcc ttatcaatct cgattttgcc gacgtgaaaa
     6121 cagttatggc aaataaaggg aatgccttaa tggggattgg gattggttct ggagaagagc
     6181 gcattgttga ggcggcgcgt aaggcaatct attcacccct attagaaacg actattgatg
     6241 gtgcacaaga cgttattgtg aacgttacag gaggtctcga catgacactc acagaagctg
     6301 aagaagcctc tgaaattgtt gggcaagctg ctggtcaagg cgttaacatt tggttaggaa
     6361 catctattga tgatactatg aaagatgaca tccgtgtgac tgttgtagca actggagtgc
     6421 gccaagaaaa agccgaacaa gtttcaggtt ttcgtcagcc taggactttt acccaaacca
     6481 acgcgcagca agtagcgggt gcacaatatg catcagatca agcaaaacag tcggttcaac
     6541 cagggtttga tcgtcgctca aattttgatt ttgacatggg ggagtctcgc gagataccaa
     6601 gtgcacaaaa ggtaatttct aatcataatc aaaatcaagg ttctgctttt ggaaattggg
     6661 atttgagacg tgataatatt tctcgtccaa cagaaggtga attggataac catcttaata
     6721 tgtcaacgtt ctcagctaac gatgacagtg atgatgaatt agaaacgcct ccattcttta
     6781 aaaaccgtta ataatggatt tactgacaaa taaaaagaaa atttttgaga ctatccgctt
     6841 atctacagag gcagcaaata ggactaatga tagtgtttca gttattgctg taacaaaata
     6901 tgtggatagt acaattgcag gtcagcttat cgaagcagga attgagcaca ttgccgaaaa
     6961 ccgtgttgat aaatttcttg aaaagtatga tgcgttaaag tatatgccag taaagtggca
     7021 tttaatcggt accttacaac gtcgtaaagt caaggaagtt atcaattatg ttgattattt
     7081 tcacgctcta gattctgtga gattagcttt ggaaatcaac aagagagctg accatcctgt
     7141 gaagtgtttt ctacaagtta atatttctaa agaagagagt aaacatggtt ttaacatttc
     7201 tgagattgat gaagcgattg aagaaatagg taagatggag aagatacagt tagttggttt
     7261 aatgactatg gcaccagcaa atgccagtaa agaaagtatt ataactattt ttcgacaagc
     7321 aaatcaatta agaaaaaact tgcagttaaa aaaaagaaag aatatgcctt ttacagaatt
     7381 gagcatgggc atgagtaacg attatccaat tgctattcaa gaaggctcaa cttttattcg
     7441 gattggtaga gctttctttc actaatggag aataagatgg cttttaaaga tacatttaac
     7501 aagatgattt cttattttga cacggatgag gttaacgaag ttgaagaaga tgttgcagca
     7561 tcaactgata acgttattcc aagatcacaa caatcagtca gagcaagtag tcatccaaaa
     7621 caagaaccta gaaacaatca cgtacaacaa gatcatcagg cgagatccca agaacagaca
     7681 aggtcacaaa tgcatccaaa acatggtact tctgaacgct attatcagca gtctcagcca
     7741 aaagaaggcc atgaaatggt tgacagaaga aaacggatga gcacttctgg tattgcaaat
     7801 cgccgtgagc agtatcaaca atcaacttgt tcagatcaga caactattgc cttaaaatat
     7861 cctcgtaaat atgaggatgc tcaagaaatt gtggatcttt taatagttaa tgaatgcgtt
     7921 ttgattgatt ttcagtttat gctagatgct caggctagac ggtgtttaga ttttattgat
     7981 ggtgctagta aagtgctcta tggtagctta caaaaggtcg gctcttcaat gtacttactg
     8041 gctccgtcaa atgtatccgt caatatagaa gaaatgacta tcccacatac tacacaagat
     8101 attggctttg attttgatat gaaaaggcgg taaataaatg atattaatac tatctattct
     8161 tcttcgtctg atcaaagttt acacttattt attgattgct tacgcattaa tgtcatggtt
     8221 tcctggggca tatgattcaa aaattgggcg tttgattagt ggtatcgttg aaccaatttt
     8281 aaaacctttt agagcattta atttacaatt tgccggtctt gacttcacta tttttgtcgt
     8341 cattattagt ttgaattttt tagctcaagt tttggtccgt gtgtttattt aatggttagt
     8401 catagtaaga tttatcagca ttttcaccaa gaagaatatc cttttattga tagaatgtct
     8461 gatatgatta atagagttgg agattactat cttttagaag ttactgagtt tttaaatcct
     8521 agagaagtga tgattttaaa aagtttgatt gctttaacag atctaaaaat gttcgtatca
     8581 acagattact acccaagcga atatggtcgt gtcattattg cacctggtta ctatgactta
     8641 gaacaaagtg attttcaaat agctttagta gagataagtt atcaggcaaa gtttaatcag
     8701 ttgacacata gtcaaatttt aggaacttta attaatgaat taggagtaaa gcgaaattta
     8761 tttggagatg tttttgttga aatgggatat gcccagctca tgattaagcg ggagttattg
     8821 gattattttt taggaacaat tactaaaata gctaaaacta gtgtgaaatt aagagaagtt
     8881 aactttgatc agttaattag gtctattgat aacagccaga ccctggatat tctagtttct
     8941 agttttcgat tagatggtgt agttgctact atcttaaaaa aatctcgaac gcaagttata
     9001 gcattaattg aagcaaataa gattaaggta aactatcgac tcgctaataa agcttcagat
     9061 aatctagtca taggggatat ggtgagtatc agaggtcacg ggcgttttac tcttttagca
     9121 gataatggag tgaccaaaca tggcaaacaa aaaataacac taagtaaaat gatacataaa
     9181 taaggagata caatggcact tacaacgcta gaaattaaag ataaaacttt taagacaaaa
     9241 ttccgaggat actgtgaaga agaagtcaat gaattccttg atattgttgt tgatgattac
     9301 gaagctcttg tacgtaaaaa tcgtgataac gaagcaagaa ttaaagatct tgaagaaaaa
     9361 ttatcttact ttgatgaaat gaaagagtca cttagccagt ctgtaatctt agctcaagaa
     9421 acagctgaaa aagtgaaagc aacggctaat gcggaagcaa caaacttggt tagtaaagcg
     9481 acttatgatg ctcagcattt attagatgaa tctaaagcta aagccaatca aatgttgcgt
     9541 gatgcaactg atgaggctaa gcgagttgcg attgaaacag aagaactaaa gcgtcaaaca
     9601 cgtgtgttcc accaacgttt aatctcatct attgagtcac aattaagctt atcaaattca
     9661 cctgaatggg atgaactgct acaaccgact gcaatctatc ttcaaaattc tgacgatgct
     9721 tttaaggaag tcgtgaagac cgttcttaat gaagacattc ctgaatctga tgatagtgcc
     9781 tcttttgatg caacacgtca gtttactcca gaagagttag aagaattgca acgtcgtgtt
     9841 gatgaaagca ataaggagtt agaggcttac caacttgact ctcaatctga ttctacgact
     9901 gagccagagg taaatctcag tgaaacacaa acgtttaaat taaatatcta aataaagaaa
     9961 aacaagatga tttgcttata atcacagcaa gcgggagatg atgagagtct cgtattccct
    10021 aagtattatc attatcattt tctaaaattt tcgctgaact acagtaagcg aggaggtttg
    10081 ttcaccttac gagcaaagag ggagcggtaa gtcttatgat atattatcgc tctgaactaa
    10141 ggtggtacca cgagctttcg tcctttttga gacgaggctt ttttcgttat agtcgcaagt
    10201 ttgtttaaaa ggagtttgaa ataatgaaat taaaagagac acttaatcta ggaaaaacag
    10261 cctttccaat gcgcgcaggt cttcctaata aagagccaca atggcaagca gcttgggaac
    10321 aagcagagct ttacaaaaaa cgtcaagaat tgaatgcagg taagcctgcc tttcatcttc
    10381 atgatggacc tccatacgct aacggcaata ttcacgttgg gcatgccctt aataaaattt
    10441 caaaagatat cattgtgcgt tctaagtcca tgtctggctt tcaagcgcct tatgtacctg
    10501 gttgggacac acacggcctt ccaatcgaac aagtcttggc taagcaagga atcaaacgta
    10561 aggagatgga tttagcagaa taccttgaga tgtgtcgtca atacgcttta agtcaggttg
    10621 ataaacagcg agatgatttc aaacggttag gtgtttcggc agactgggaa aatccttatg
    10681 tcactttgga cccacagttt gaagctgatc aaattcgtgt ttttggagcg atggctgaaa
    10741 aaggttacat ctatcgtggt gctaaacctg tttattggtc atggtcttca gaatcggcct
    10801 tggcagaagc tgaaattgaa taccatgaca tcgattcaac ctctttatac tacgccaata
    10861 aagttaaaga tggtaaaggt atccttgata ctaatacgta tatcgttgtt tggacaacaa
    10921 caccatttac ggttacagca tcacgcggtt tgactgttgg tcctgatatg gattatcttg
    10981 tggtgaaacc tgctggttca gaccgtcaat atgttgtggc agaaggtctt ttggatagcc
    11041 ttgctggaaa atttggctgg gaatcttttg aaactttagc tagccataaa ggagctgatt
    11101 tagagtacat tgtgacagaa cacccatggg atactgacgt agaagaattg gttatccttg
    11161 gtgaccatgt tacccttgag tcagggacag ggattgtcca cacagcacca ggttttggtg
    11221 aggatgacta caatgttggg acaaaatata aactggaagt tgctgtgact gttgatgaac
    11281 gtggcctaat gacggaaaat gcaggtccag atttccacgg acaattttat aacaaggtaa
    11341 cgccgattgt cattgataaa cttggtgatc ttttattggc acaagaagtg atcaatcact
    11401 cttacccatt tgactggcgg acgaaaaaac caatcatctg gcgtgctgta ccgcaatggt
    11461 ttgcttctgt ttctgatttc cgtcaagata ttttggatga aattgaaaaa acaacctttc
    11521 atccatcatg gggtgaaaca cgcctttaca atatgattcg tgatcgtggc gactgggtta
    11581 tttctcgtca acgtgcttgg ggagttccgc ttcctatctt ctatgccgaa gatggcacag
    11641 ctattatgac taaagaagtg acagatcatg tggctgattt gttccaagaa aatggatcaa
    11701 tcatttggtg gcaaaaagaa gctaaagacc tattaccaga gggcttcact catccagggt
    11761 caccaaatgg cgagtttaca aaagaaactg atatcatgga cgtttggttt gactctggtt
    11821 cttcttggaa tggagtcatg aatactaaag aaaatctttc ctatccagcg gatctctact
    11881 tagaaggctc tgaccagtat cgtggttggt tcaattcatc cttaattaca tctgtggctg
    11941 ttaatgggca tgctccatat aaggctattt tatcgcaagg ctttgttctt gatggtaagg
    12001 gcgaaaaaat gtctaaatca aaaggcaata tcatttctcc taatgatgtg gctaaacaat
    12061 atggtgcaga tattcttcga ctttgggtag cttctgtgga taccgataat gatgtccgtg
    12121 tttctatgga gatccttggt caagtctctg aaacttaccg taaaatccgt aacacccttc
    12181 gcttcttgat tgctaataca tctgatttca accctgctac ggatacagta gcttatgcag
    12241 atttaggagc tgttgataag tacatgacaa ttgtctttaa tcagttggta gcaacgatta
    12301 ctgacgctta tgagcgttat gactttatgg ctatttacaa agcagtggtg aactttgtta
    12361 cggttgattt atcagccttc tatcttgatt ttgctaaaga cgttgtctat attgaagctg
    12421 ccaatagctt agaacgtcgt cgcatgcaga cagtcttcta tgatatcttg gttaagatta
    12481 ccaagttatt gacgccaatc ttgcctcaca ctactgaaga aatctggtct tatctagagc
    12541 atgagtcaga agcatttgtt caattggcag aaatgcctgt ggcagaaact ttctcagctc
    12601 aagaggatat tttagaagct tggtctgctt tcatgacttt gcgtacccaa gctcaaaaag
    12661 ctttggaaga agcgcgaaat gctaagatta tcggtaaatc attggaagcc catttgacca
    12721 tctacgctag cgaagaagtg aaaaccttat tgactgcttt agacagcgat attgctttgc
    12781 ttttgatcgt gtctcaatta accattgctg acttggcaga tgcgcctgcg gatgcagtgg
    12841 catttgaagg tattgccttt atggtagaac atgccatagg tgaagtttgt gagcgttcac
    12901 gccgcatcga cccaactact cgcatgcgtt cttacaatgc atttgtctgt gatcacagcg
    12961 ctaaaatcat tgaagaaaat ttcccagaag ctgtggctga agggtttgaa gagagtgaca
    13021 aataagtctt atcaagactt gaagtgacac tgagtaagaa gacaaccatc gggtcttagg
    13081 gcctgatggt tttttagtag agctgattac gtccattagc cagctttaac gagagacaaa
    13141 gtaggagaaa tttagagtac ttttttaatt tcttttaaga aaattgctgg cttcatataa
    13201 ggactattaa gatagagttc acctttcgct aataaaaaaa cttagctctt ttagaagggc
    13261 taagttttgt ctgaatagct ggctgctgtt tatacagaag tttgaaatga aatttcaata
    13321 aggttattcg tgtgaagtgt ttttgattgt ccaggaataa caattttagg atcaatttta
    13381 cgttttaaaa aaaattcgcg aatgatatca agttcatcct ccttgatggc acggtattta
    13441 tttgaaataa gtagttcgta atgttttggt gcttgggtaa aaatgacatc tgtatttcct
    13501 gctgagtata cttcgactaa ataggcatcg gtgtttttta gctgatttct gatgagttct
    13561 gggtgactgc tagtggtatt gatgagtctc ataaatgtcc tccttagtat tcctgtacct
    13621 attatagcac taatttaaag ccttagttag tagaatgatg ctttttttgc cattccttga
    13681 ctccccattg atggctctct cgttttaatt tagcaattgc ctcgatagga gaaaaccaac
    13741 ttaagttatt aaaatcttct aagggcttag aaacggcttg aaatgcggtt acttcataaa
    13801 gataggcagg atggtagaag tgggtatcac gatggcgaga gtagaaatat tcatcagcct
    13861 gaccgtaata tgaaccaata gtagcagaaa aaccaagttc ttcaatcagt tcacgctcta
    13921 gggcctgaag ctgaccttca cctgcttcaa tttcaccacc aggtaaaaac caggaaccat
    13981 taggagcttg aacaagaata atttttgttt gttcgtgatt gggaatgata gcatagacac
    14041 cgtaacgtgt gacatagtct ttatgtgcat ttttatgtcc aaatgttgga atcatcattt
    14101 tatttacctt tcctttttct tcattatact aaatttttgc taattattct tatcaatttg
    14161 tttagtttaa taaaaaattg atagaaaaag agagagtctc cttatagaaa ctctcttagc
    14221 taacgttaat ctagagactc ttcgttaaga aaagaatctc cctctttaac agctttaatc
    14281 gtgatgtttt cactgtttgt catcagtgca cgtaactgtt tttcagttgg gtgttctaaa
    14341 taatagtctg taatggcatc ttcgatataa tcttggatag tacgacggag cggccgagcg
    14401 cccattttag gatcatagcc taaatctact aatttttctt tgaccttttg tgtcacatca
    14461 agatgaatcc cattatagcc taagcgctca ttaacatctt ccagcattaa atcaacgata
    14521 tgcagcaaat gctctttaga taaagctttg aattcaataa taccatcgaa acgattcata
    14581 aactctgggc tgaagaagtt gcttaattca ccaaggacag aacttgtccg tccttctcta
    14641 gcagcaccaa atccgacaga agcttcgctt ttacctgttc cggcattaga ggtcatgata
    14701 ataatggtgt ccttgaagct gactgttcgc ccttggccgt ctgttaagcg accgtcatca
    14761 agaacttgta agaacatgtg catgacgtca ggatgggctt tttccacctc atctaagaga
    14821 ataagggaat aaggattgcg acgaacttgt tcggttagct gtccagcttc ttcatagccg
    14881 acataacctg gaggagcccc gactaatttg gcgacagcgt gtttttccat gtattcggac
    14941 atgtcaaagc gaatcatatt gtttgtcgaa ccaaagagtt caatggctag ttgtttagac
    15001 aattcagttt taccaacccc agtcggtcca acgaataaga aagaaccaat agggcgattt
    15061 ggagttccta atcccacacg gttacgacga atagccttag cgattttatc aacagcatcg
    15121 tcttgcccga tcacgtgtgc tttcagatca ttggctaagt ttacgagttg agactgttcc
    15181 ttttctttta agtccccaac tggaatatta gttttttgct ctacaatagc ttcaatggtt
    15241 ttttcagtaa tgatgggaat atcttgctca tcgacttttt gagcctgcat ttctttgtat
    15301 tttgtaattt gatcgcggaa ataagctgcg cgttcatagt cttcgtctcg agtagcttgc
    15361 gccttgagat tctcagcttc aataagacgt ttgtcaattt ctttaggatc aacaaagttg
    15421 agggttagat tcattttgga accagcttcg tccagaaggt caatagcctt atcaggaaga
    15481 aaacggtctt gaatatagcg gttagataaa tgagcggcag cttcaatagc ggctgggcta
    15541 tatttgacat gatgatagtc ttcgtatttc ggttggatac cttttaaaat cgtgatggtt
    15601 tcctcaacag aaggttcatc aactttaacg ggttgcatac gtcgttctaa ggcagcgtct
    15661 ttttcaataa tacggtattc atttaatgta gtagcaccaa cgagttgcaa ctcaccacgg
    15721 gccaaggctg gttttaaaat attaccagca tccatattgc cgtctcctgc agaaccagca
    15781 ccgacaatct catgaatttc atcaataaag agaatcacat ccttgcgatt gcgaatttct
    15841 tccattaatt tttgcatgcg ctcttcaaac tgaccacgga tacctgttcc ctgaacgagg
    15901 ctaaccacat caagacgaat cacttgcttg ccttggagtt tttgaggaac agtaccatca
    15961 ataatttttt gagccaaacc ttctacaaca gcagttttac caactccagg ctcaccaatt
    16021 agcacaggat tatttttagt acggcggttg aggatctcga taacgcgtgt aatctcttcg
    16081 tcacgaccaa taacaggatc aatattacca tttcttgcaa tatctgtgac attaatccca
    16141 aactcttcta ataagccctt tgcttgctga tttggtgttt gtggctgagc aggtcgttgt
    16201 ccgttgtagt taccaccata gcgtcctccg ccatttccat tttgccctgc ctgagtaggt
    16261 ggggtatttg gaagattacc aaaagctcta aaattattca aatcaccaaa aaagtcatca
    16321 aagaaaggac ttgtggatct atcttgtgct ctatatcctg gggttaggcc atttaaaata
    16381 gaattggcag gatcggattt catgatttga taacaatttt gacagagatc aacctgtctt
    16441 tgttttccat taacacttgt ataaagatga atagtagatt cgtttaaatt acaattttga
    16501 cacagcataa tatacctccg agggtttatc taaaagtcaa agtcatttag tcaaaaatgg
    16561 tcaaacttta tattcatatt atagcaccac ggcttgggaa atcaagtaaa aagtttatcg
    16621 ctttagcgtt agcttttaga gagtaacatg acagagtaac tttattaagt ttccaatgag
    16681 caaatgttta aaatcattga aaagagtaaa aaaagtaaaa tatcaataaa tatattactt
    16741 tccttgttag tttcccttgt tttgtgttaa aataagaggg ataaaaaacg aataggagaa
    16801 acgaaatgga atcacatttg gtgagaatca tcaatcgttt ggagttgatg gtgacagatg
    16861 gcggtaatct caagcgaaat tttgaacgcg atggtgttgt tgttgcagaa gttgcctttt
    16921 caaatgatcc tgaaaatgga cctgttttca cattgcgtga tgttgaagcg cgtgagtcat
    16981 attcctttga tagcattgac ttgattgcaa tggaaatcta cgatttatta tattaagctt
    17041 gacatagtaa agagacttgc ctgaggcagg tttttttatt gtcaaaaagt attgtattaa
    17101 tattcatcag atggtataat tatctttatt gtaatgaacc taggacttat gaggagatat
    17161 aaatggattt gtcatttttg cccaaatact gggcctactt taactacggt gtacttgtca
    17221 ccattatgat ttcagtcagc gttgtctttt ttggaaccct tattggtgtc ttggtaaccc
    17281 tgattaagcg tagtcatgtg aagccgttga cctgggtcgt taatctttac gtttggatct
    17341 ttcggggaac acctatggtg gttcaaatca tgattgcctt tgcttggatg cattttaaca
    17401 atatgcctac tattggtttt ggggttttag acttggactt ttcaagacta cttcctggaa
    17461 ttattatcat ttcattgaat agcggtgctt atatttcaga aattgttaga gcaggtattg
    17521 aggctgtacc aaaagggcaa ttagaagcag cttattcact aggtattcgt cctcaaaatg
    17581 ccatgcgtta tgtgattttg cctcaggcct ttaaaaatat tttgccagcc ttaggaaatg
    17641 aatttattac cattattaag gatagtgctt ttttacaaac cattggagtg atggaacttt
    17701 ggaatggtgc ccaatcagtg gtaacggcta cttattctcc aatttcccct ttactggtgg
    17761 ctgcttttta ctacttaatg gtcacaacag tgatggcaca gttattggca gtcttagaac
    17821 gtcacatggc gcaaggaggt aatcattgat gactgaggcc attatttcaa ttaaagactt
    17881 gcacaagtat tatgggcaca atgaagtgct taagggaatt gatttagaca ttatgccagg
    17941 agaggttgtg gtgattattg gcccgtctgg ttctggaaaa tcgaccttac ttcgaaccat
    18001 gaatcttttg gaagtaccaa ctaagggaca gattaggttt gaggggattg atattaccga
    18061 taagaagaac gatattttca gcatgcgtga aaaaatgggg atggtttttc agcagtttaa
    18121 cctctttccc aatatgactg ttttagaaaa tatcacttta tcgccaatca aaactaaggg
    18181 aatggctaaa gcagaggctg acaaaacagc cttgagcttg ttggacaaag ttggattatc
    18241 agaaaaagcc aaggcttatc ctgctagtct ttctggtggg caacagcagc ggattgcgat
    18301 tgcgcgtgga ctggctatgg atccagatgt tttactcttt gatgaaccaa cttcagctct
    18361 agacccagaa atggtgggtg aggtcttggc tgtcatgcaa gatttggcta aatctgggat
    18421 gactatggtt attgtgactc atgaaatggg ctttgctaaa gaagtcgcag atcgtgtgat
    18481 ttttatggat ggtggcgtta ttgtggaaga aggaagtcct aaccaacttt ttgatctgac
    18541 taaagaagaa cgtacaaaag attttttgag tagagtatta taaatctgtt ctatgaatta
    18601 taaaaggaag tcctaaatca tttaggactt ccttttataa tttgctaagg cattttatag
    18661 gagattact
//
LOCUS       DATUYR010000033        16191 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.033, whole genome shotgun sequence.
ACCESSION   DATUYR010000033 DATUYR010000000
VERSION     DATUYR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.033"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            128..3154
                     /locus_tag="VCI33_001085"
     CDS             128..3154
                     /locus_tag="VCI33_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEP2832015.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTIGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINRAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            3174..3575
                     /locus_tag="VCI33_001086"
     CDS             3174..3575
                     /locus_tag="VCI33_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEP2832016.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(3765..4640)
                     /locus_tag="VCI33_001087"
     CDS             complement(3765..4640)
                     /locus_tag="VCI33_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEP2832017.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLKESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSGIEVN"
     gene            4776..5297
                     /locus_tag="VCI33_001088"
     CDS             4776..5297
                     /locus_tag="VCI33_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP2832018.1"
                     /translation="MTKDRQIKKTKTAIYSAFLALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLELIK"
     gene            5512..6030
                     /gene="rimM"
                     /locus_tag="VCI33_001089"
     CDS             5512..6030
                     /gene="rimM"
                     /locus_tag="VCI33_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEP2832019.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DQFVQEVTIVSHRKQKHFDIIKLKDMYHINAIEKYKGYTLKVSKANQGDLQEGEFYYH
                     QIIGMAVYEKDRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            6020..6751
                     /gene="trmD"
                     /locus_tag="VCI33_001090"
     CDS             6020..6751
                     /gene="trmD"
                     /locus_tag="VCI33_001090"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEP2832020.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALGQGED"
     gene            6751..7743
                     /locus_tag="VCI33_001091"
     CDS             6751..7743
                     /locus_tag="VCI33_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP2832021.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGVELTENLIKQLNRFEDRITICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLTTSTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            7922..8980
                     /locus_tag="VCI33_001092"
     CDS             7922..8980
                     /locus_tag="VCI33_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEP2832022.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            8993..9916
                     /locus_tag="VCI33_001093"
     CDS             8993..9916
                     /locus_tag="VCI33_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEP2832023.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     VVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDAGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            10172..10885
                     /locus_tag="VCI33_001094"
     CDS             10172..10885
                     /locus_tag="VCI33_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP2832024.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKTIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGIDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            10882..11793
                     /gene="pfkB"
                     /locus_tag="VCI33_001095"
     CDS             10882..11793
                     /gene="pfkB"
                     /locus_tag="VCI33_001095"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEP2832025.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETEIN
                     GQGPVISREQLEDLKTKLSQLTAEDTVVFAGSAPANLGNAVYKELFPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            11790..13736
                     /locus_tag="VCI33_001096"
     CDS             11790..13736
                     /locus_tag="VCI33_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP2832026.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            13829..14428
                     /locus_tag="VCI33_001097"
     CDS             13829..14428
                     /locus_tag="VCI33_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP2832027.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYNTKLNELIEWYQLTNFDGLMK"
     gene            14580..15287
                     /locus_tag="VCI33_001098"
     CDS             14580..15287
                     /locus_tag="VCI33_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP2832028.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(15344..15661)
                     /locus_tag="VCI33_001099"
                     /pseudo
     CDS             complement(15344..15661)
                     /locus_tag="VCI33_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(15859..>16191)
                     /gene="spej"
                     /locus_tag="VCI33_001100"
     CDS             complement(15859..>16191)
                     /gene="spej"
                     /locus_tag="VCI33_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeJ"
                     /protein_id="HEP2832029.1"
                     /translation="SKIVGNLLIDGVQQKTLINPIKIDKRIFTIQEFDFKIRQYLMQT
                     YKIYDPNSPYIKGQLEIAINGNKHESFNLYDATSSSTRSDIFKKYKDNKTINMKDFSH
                     FDIYLWTK"
BASE COUNT         5202 a         2606 c         3321 g         5062 t
ORIGIN      
        1 ttaaaataag tgatagactt ggatattatt aggtcgacta gtgttctttt caataattta
       61 atatcgttat tattagtatc agccaatcaa atgtggataa gttggctttt agaaaggttt
      121 taccacaatg aagaaacatc ttaaaacagt tgccttgacc ctcactacag tatcggtagt
      181 cacccacaat caggaagttt ttagtttagt caaagagccg attcttaaac aaactcaagc
      241 ttcttcatcg atttctggcg ctgactacgc agaaagtagc ggtaaaagca agttaaagat
      301 taatgaaact tctggccctg ttgatgatac agtcactgac ttattttcgg ataaacgtac
      361 tactcctgaa aaaataaaag ataatcttgc taaaggtccg agagaacaag agttaaaggc
      421 agtaacagag aatacagaat cagaaaagca gatcacttct ggatctcaac tagaacaatc
      481 aaaagagtct ctttctttaa ataaaacagt gccatcaacg tctaattggg agatttgtga
      541 ttttattact aaggggaata cccttgttgg tctttcaaaa tcaggtgttg aaaagttatc
      601 tcaaactgat catctcgtat tgcctagtca agcagcagat ggaactcaat tgatacaagt
      661 agctagtttt gcttttactc cagataaaaa gacggcaatt gcagaatata ccagtagggc
      721 tggagaaaat ggggaaataa gccaactaga tgtggatgga aaagaaatta ttaacgaagg
      781 tgaggttttt aattcttatc tactaaagaa ggtaacaatc ccaactggtt ataaacatat
      841 tggtcaagat gcttttgtgg acaataagaa tattgctgag gttaatcttc ctgaaagcct
      901 cgagactatt tctgactatg cttttgctca cctagctttg aaacagatcg atttgccaga
      961 taatttaaaa gcgattggag aattagcttt ttttgataat caaattacag gtaaactttc
     1021 tttgccacgt cagttaatgc gattagcaga acgtgctttt aaatcaaacc atatcaaaac
     1081 aattgagttt agaggaaata gtctaaaagt gataggggaa gctagttttc aagataatga
     1141 tctgagtcaa ctaatgctac ctgacggtct tgaaaaaata gaatcagaag cttttacagg
     1201 aaatccagga gatgatcact acaataaccg tgttgttttg tggacaaaat ctggaaaaaa
     1261 tccttatggt cttgctactg aaaataccta tgttaatcct gataagtcac tatggcagga
     1321 aagtcctgag attgattata ctaaatggtt agaggaagat tttacctatc aaaaaaatag
     1381 tgttacaggt ttttcaaata aaggcttaca aaaagtaaaa cgtaataaaa acttagaaat
     1441 tccaaaacag cacaatggtg ttactattac tgaaattggt gataatgctt ttcgcaatgt
     1501 tgattttcaa aataaaactt tacgtaaata tgatttggaa gaagtaaagc ttccctcaac
     1561 tattcggaaa ataggtgctt ttgcttttca atctaataac ttgaaatctt ttgaagcaag
     1621 tgacgattta gaagagatta aagagggagc ctttatgaat aatcgtattg aaaccttgga
     1681 attaaaagat aaattagtta ctattggtga tgcggctttc catattaatc atatttatgc
     1741 cattgttctt ccagaatctg tacaagaaat agggcgttca gcatttcggc aaaatggtgc
     1801 aaataatctt atttttatgg gaagtaaggt taagaccata ggtgagatgg catttttatc
     1861 aaatagactt gaacatctgg atctttctga gcaaaaacag ttaacagaga ttcctgttca
     1921 agccttttca gacaatgcct tgaaagaagt attattacca gcatcactga aaacgattcg
     1981 agaagaagcc ttcaaaaaga atcatttaaa acaactggaa gtggcatctg ccttgtccca
     2041 tattgctttt aatgctttag atgataatga tggtgatgaa caatttgaca ataaagtggt
     2101 tgttaaaacg catcataatt cctacgcact agcagatggt gagcatttta tcgttgatcc
     2161 agataagtta tcttctacaa tggtagacct tgaaaagatt ttaaaactaa tcgaaggttt
     2221 agattattct acattacgtc agactactca aactcagttt agagacatga ctactgcagg
     2281 taaagcgttg ttgtcaaaat ctaacctccg acaaggagaa aaacaaaaat tccttcaaga
     2341 agcacaattt ttccttggcc gcgttgattt ggataaagcc atagctaaag ctgagaaggc
     2401 tttagtgacc aagaaggcaa caaagaatgg tcagttgctt gaaagaagta ttaacagagc
     2461 ggtattagct tataataata gcgctattaa aaaagctaat gttaagcgct tggaaaaaga
     2521 gttagacttg ctaacaggat tagttgaggg aaaaggacca ttagcgcaag ctacaatggt
     2581 acaaggagtt tatttattaa agacgccttt gccattgcca gagtattata tcggattgaa
     2641 cgtttatttt gacaagtctg gaaaattgat ttatgcactt gatatgagtg atactattgg
     2701 cgagggacaa aaagacgctt atggtaatcc tatattaaat gttgacgagg ataatgaagg
     2761 ttatcatgcc ttggcagttg ccactttagc tgattatgag gggctcgaca tcaaaacaat
     2821 tttaaatagt aagcttagtc aattaacatc tattcgtcag gtaccgactg cagcctatca
     2881 tagagccggt attttccaag ctatccaaaa tgcagcggca gaagcagagc agttattgcc
     2941 taaaccaggt acgcactctg agaaatcaag ctcaagtgaa tctgctaact ctaaagatag
     3001 aggattgcaa tcaaacccaa aaacgaatag aggacgacac tctgcaatat tgcctaggac
     3061 agggtcaaaa ggcagctttg tctatggaat cttaggttac actagcgttg ctttactgtc
     3121 actaataact gctataaaaa agaaaaaata ttaaattgat ttaaggagaa gaaatgataa
     3181 cacaagaaat gaaagatctt attaataacc aattagcaat ggttgcgacg gttgatgcca
     3241 aaggtcaacc caatattggt ccaaaacgtt ctatgcgtct ctgggatgat aaaacattta
     3301 tttataatga aaatacagat ggtcaaactc gtattaacat tgaagataat gggaaaattg
     3361 aaattgcctt tgttgatcgt gagcgccttt taggttacag gtttgtagga actgctgaaa
     3421 ttcaaacaga aggtgcttat tacgaagcag cgaaaaaatg ggcacaagga cgcatggggg
     3481 taccaaaagc agttggtatt attcatgtcg agcgtatttt taatttacaa tctggtgcta
     3541 atgcaggtaa agaaataaac agtgaaagta actaaaatta actaaaatta actgtaacag
     3601 tatttaggac ccttatttta aatgctaaaa aacaagcata ctctttttta cagcatagtg
     3661 actatgttgt aaaaaggggt atgcttatta ggtgctctta atatttctag ataactgcac
     3721 ctaataagtt ttcaatagac ttatagcaca ttagttgact tcaattagtt gacttcaata
     3781 ccagaaagag aagaacagtg atgttggtgt tcgtttaaag aagtatcgat ttccacagtg
     3841 actgagttta tattgtacct ttgacaaatc agtctaatag attttttgca atgtttttca
     3901 gtggtagatt ctttcaagca gcaatgtata gttgctctat ggtcaatacc atccatcgac
     3961 caaacattaa gctgattaac agacactata tgaggaagtt ggctgagttc atggtgcaaa
     4021 gcgcaatagt ctatcgaatc aggaacacca tctaaaaaaa tgttagcggt tgctactaac
     4081 tttggaagag ctttagataa tataaaagaa gcaatggcaa ttgataataa aggatcaaga
     4141 atgtaccaag gtttccattt taagatcagt gacagtatga taatggctaa ccatccaagg
     4201 atatcttcta aaaagtgaag gcttaatatt tcttcatttt ttgtctggtt agagtgaatg
     4261 atgaagctag caaatccatt gattataatt gcaaaaatag ctaaaacgaa cataccgtca
     4321 taattaacaa tagtaggatg ccataatttt ggtatcgttt caatcattac taataatgac
     4381 ccgctaataa gtattagatt tgtcgttagc gctcctaaaa ggctaaatcg cttatagcct
     4441 agtgaaaaat ttggactctc ttttttaaca gcctttcttt ctaagatagc tgagatacca
     4501 atagcaatgg catctccaaa atcgtggaca gcatctgcta aaatagcacc cgaaaagaat
     4561 aatgttccaa aaataaattc aatgagggaa aaactaagat ttaatataaa aataatggtg
     4621 acttttttac ttgctggcat aattttctcc ttttgttata ctgataatat gtatagtaga
     4681 attctttatt ctattatcta aaagttaaaa tacaattact ataaccaaaa atgagcacct
     4741 gttcaatagt gaacaacatt tagaaagtgt ccgatatgac aaaagacaga caaatcaaaa
     4801 aaacaaaaac agctatctat agtgcttttc ttgctttatt acaaaaaaaa gaatattcga
     4861 aaataactgt tcgagatatg attacacttg ctaatgtagg gagatcaacc ttttatgcac
     4921 attatgaaag caaagaaatg ttactaaaag aactctgtga agaacttttt catcatttgt
     4981 ttaggcaaaa aagaaatgtg acttttgagg actatttggt acatatttta aagcattttg
     5041 aacaaaataa agacagcatt gcgacgttgt tactttctaa tgatccctat tttttattaa
     5101 gatttaaaaa cgaactagaa catgatgttt accctaactt gcgctgcaag tatatcgaca
     5161 aaacgactat tccagaagta ttcctgaaac aatttgttct atctagtttt atagaaacct
     5221 taaaatggtg gctccatcaa aggcaaagga tgtctgcaaa tgaactatta aagtattatt
     5281 tagagctcat aaaataatga ctagtgagtc atcttgccaa attcaatttt ttaatacaga
     5341 cggattttaa caatttaaca gagaaagaca aaactgtaag tgcaaaatag actataacgt
     5401 gataaaataa taggtaattt ggttctgaaa aagaagtgtc tatcaaactt tagaatcaag
     5461 agaggtcgct aaagtgacgt gagataaaga gttaacgatt taggagctac catggaatat
     5521 tttaatgttg gaaaaattgt caacacgcaa ggcctgcaag gagagatgcg cgtgttgtct
     5581 gtcagtgatt ttgctgagga gcgctttaag aagggatcgc aattggcttt gtttgatgac
     5641 aaagaccagt ttgttcaaga agtgacaatt gttagtcacc gtaaacaaaa gcattttgat
     5701 attatcaaat tgaaagacat gtatcatatt aatgcgattg aaaaatataa gggctatacg
     5761 ttaaaagtgt caaaagctaa tcaaggagac ctgcaagaag gagaatttta ctaccatcag
     5821 attatcggta tggctgttta tgaaaaggac cggttgattg gctatgtcaa ggaaatcttg
     5881 cagccaggtg ctaatgatgt ttgggtagtg aaacgccaag gaaaacgaga cttgctctta
     5941 ccatatattc ccccagtagt tcttaacgta gatgttccta acaagcgcgt agatgttgaa
     6001 ttgatggaag gcttagacga tgaagattga tattttaacc ctttttcctg aaatgtttgc
     6061 ccccctagaa cattccattg taggcaaagc aaaagaaaaa ggattacttg acattcacta
     6121 ccataacttt cgagattatg ctgaaaaagc tcgtcatgtg gatgacgaac cctatggtgg
     6181 gggccaaggc atgttattga gagcacagcc catttttgac accattgaac agattgaagc
     6241 caaaaaacca aggattattc ttttagatcc agcaggtaaa ccctttactc aggcttatgc
     6301 tgaagaatta gctcttgagg aagagttaat ttttatttgt ggtcattatg aaggttatga
     6361 tgagcggatt aagaccttgg tgacggatga aatttcatta ggtgactttg tattaacagg
     6421 aggtgagttg gcggccatga ctatggtgga tgcgactgtc aggttaattc cacaggtact
     6481 tgggaaagaa agtagccatc aggacgattc tttttcatca ggacttttag aataccctca
     6541 atacacaaga ccttatgatt atcgtggtat gacagttcca gatgttctaa tgagtggtca
     6601 tcacgaacgt attcgcttgt ggcgtctaga agaaagtctc agaaaaacct atttgagaag
     6661 gccagatttg ttagagcggt acgatttttc cgaggaagag cgcaagctat tggataagat
     6721 taaagaagca ttgggtcaag gagaagatta gtgaaagata aagcatatga tattacgatt
     6781 attggtggtg gtccaatagg tttatttgca gccttttatg caggtcttag gggcgtaaca
     6841 gtaaagatta ttgagagttt gtcagaactt ggtggacagc ctgccattct ttatcctgag
     6901 aaaatgattt atgatattcc agcttatcca tctttaacag gtgttgagtt gacagaaaat
     6961 ttaataaaac aattaaaccg ttttgaagat cgtataacca tttgtttaaa agaagaagta
     7021 ttaacctttg acaaggttaa aggtggtttt tcgattagga ctaataaggc agagcatttt
     7081 tcaaaagcca ttatcattgc ttgtggaaat ggcgcttttg caccaagaac tttagggtta
     7141 gaaagtgagg aaaactttgc tgatcataac cttttttata atgttcacca gttggaccag
     7201 tttgcgggac aaaaagttgt gatttgtggt ggtggagatt cagcagttga ttgggctttg
     7261 gctttagaag atattgctga gagtgtgacg gttgtgcacc gtcgtgatgc tttcagagca
     7321 catgaacaca gtgtggaatt attgacaaca tcaacggtaa acctcttaac accttatgtg
     7381 ccaaaagcat tgaaaggaat tggaaacctg gctgaaaaat tagttattca aaaagtgaaa
     7441 gaagatgaag tgttagaact tgaattggac agtcttattg ttagttttgg tttttcaact
     7501 tctaataaga atttgaaaaa ttggaatctc gactataagc gttcaagtat tactgtttca
     7561 ccactttttc aaacaagtca agaaggtatt tttgccattg gtgatgcagc cgcttataat
     7621 gggaaagtgg atttgattgc tactggtttt ggcgaagcac caacagcagt taatcaggcc
     7681 attaactata tttatccaga tcgagataac cgtgtggtgc attcaacctc tttaattgac
     7741 taagggagtc ggaagtttca agagcagtag caacagctaa tattcagttt gtcaggagag
     7801 gacaaatggt tttttaccta agtcttcgtg acaagtttta tactttgtga taaaatagtc
     7861 attgtgaaaa aagtgtgatg gttttcacag attaacacat tacaatgaaa aggggtctat
     7921 catgacaaca acagataaag agacatttag ttcttttatg aataaggtct tggctggtac
     7981 agcgattgct atcgttgtgg cactcattcc aaatgctatt ttagcgactt tcttaaaacc
     8041 gcttttgcca aacatggcag ctgctgaatt tttacacatt gtgcaagtct tccaattctt
     8101 cacgccgatt atggctggtt tcttgattgg gcaacaattt aagtttaatc ctatgcaaca
     8161 gttggctgtt ggtggagctg cttatatcgg ttctggggct tgggcttata cagaagtcat
     8221 tcaaaaaggt gtggcgacag gaacattcca acttagaggt attggggact taatcaatat
     8281 gatgattaca gctagccttg cggttttagc tgtgaaatat tttgggaata aatttggctc
     8341 tttaaccatt atcttgttac ctatcactat aggaactggc gtaggttata ttggttggaa
     8401 atttttgcct tatgtgtctt acgtgacaac cctgatcgga caaggtatta attcttttac
     8461 aaccttacaa cctattttaa tgtctatttt aattgcagtt gctttctcgc ttatcattgt
     8521 tagtccgatt tcaactgttg caatcggttt agctattggg cttaatggaa tggctgcggg
     8581 agcagcttct atgggaattg catcgacagc agcagtctta gtgtgggcga cgcttaaagt
     8641 taataaatct ggtgtaccaa ttgctatcgc acttggggct atgaaaatga tgatgccaaa
     8701 cttcttgaaa catcctatca tggctattcc gatggtgttc actgcagcaa ttagctcatt
     8761 aacagtacca ttgtttaatc ttgttggaac accagcttca tctggttttg gcttagttgg
     8821 tgcagtaggt ccgattgctt ctttagcagg tggtagctca atacttatta ttatccttgc
     8881 ttggatcatt gttccgtttg cggttgcttt tgcggcgcat aaggtttcta aagatattct
     8941 aaaactttat aaagaagaca ttttcgtctt tgaaggctaa taggaggaaa atatgttagt
     9001 ttatattgct ggctcaggag ccatggggtg ccgttttggt taccaaattt caaaaacaaa
     9061 taacgatgtg attttgttag ataactggga agatcatatc aatgccatta aagaaaacgg
     9121 tttagtggtg acaggggatg tggaagaaac agttaagctt cctattatga aaccgactga
     9181 agccactcaa gaagccgatt tgattatctt gtttacaaaa gcaatgcagt tgccacaaat
     9241 gcttcaagac attaaaggca ttattggaaa agaaaccaaa gtgctttgcc ttttaaatgg
     9301 tcttggacac gaagacgtta ttcgtcagta cattccagag cacaacattt tgatgggggt
     9361 cactgtctgg actgctggtc tagaaggacc aggtcgtgct catcttcaag gagttggtgc
     9421 tcttaactta caaagtatgg atccaaacaa tcaagatgcg ggacatcaag tagctgactt
     9481 gttaaataaa gctaacttaa atgcgactta tgatgagaat gttgtgccaa acatctggcg
     9541 aaaagcttgc gtgaatggta ccatgaattc gacttgtgct cttttagatt gtacgattgg
     9601 cgaattattt gccagtgaag atggtctcaa aatggtaaaa gaaatcatcc atgagtttgt
     9661 tatcgttggt caagcagaag gtgtcgaact aaacgaagaa gaaatcacac aatatgtaat
     9721 ggatacatct gttaaagctg ctcaccatta cccatcaatg caccaagacc ttgtgcaaaa
     9781 tcaccgattg actgaaattg attttatcaa tggtgcagtc aataccaaag gtgaaaagct
     9841 tggtatcaat actccatact gccgtatgat tacagaacta gttcacgcta aagaagcagt
     9901 gttgaacatc caataaacgc tatcaaatcg ttatactcaa tattatggct aaagctttaa
     9961 tattgagttt tttgttatgc tgatagtatt tttcttaggc gtcattattt gtgagcctag
    10021 attatgagtt aataaaagaa ataataattg gtagaatttg atagattttg aaaggttatg
    10081 cttgactttt agttataaaa tagtataata attgtaaacg atttcagtta ggggtggttc
    10141 tatcttaaaa tcaaaacgaa aacaattaat catggcaaag atcactgaag aaaactatgt
    10201 ttctttagag gatttaatgc aattactcaa ctcctctgag tctactatcc gtagagatct
    10261 aggagagttg gagcaagaag gacggctaca tcgtgttcat ggaggtgctg aactttttca
    10321 ctctttacaa gaggaacttt ctaaccaaga aaagtctgtc aaaaacagtc atattaaaaa
    10381 aactattgcg caaagagcct ctcagtttat ctatgacaac gatgtgatct ttattgatgc
    10441 tgggacaacg actgaatttt tattaccttt tttacaagct aaaaatttaa ctgtagttac
    10501 taattctatt catcatgctg ctagattagt tgaactctct attgagacga ttattgtagg
    10561 tggttatgtc aaacaaacga ccgatgccag tattggtaat gtagctttgg aacagattag
    10621 gcagatgaat tttgacaagg catttcttgg aatgaatggt attgatgatt cttacctgac
    10681 gactcctgat atggaagaag cagttattaa aaaagcggtt ctgtcaaatg caaaattagc
    10741 ttacatttta gtagatggca ctaaaattgg tcaggtatcg tttgtaaaag tagctcctat
    10801 taatgatgtg actattatta cattaggagg atcagcaagt attcttaaac aaataaagga
    10861 aaaggcaaag gtgattgaat tatgatttat accgtgacct taaacccttc tattgacttt
    10921 attgtcagaa ttgaccaaat aaatcttggg tcagtgaatc gcatggcgag tgatgataag
    10981 tttgctggag gtaaaggcat taatgtgagt cgcatcttgc aacgtttaga cattgctagc
    11041 acggcgacag gattcttggg tggttttaca ggacgtttta tcgaagaaag tttgagtgca
    11101 gaaggcgtta aaactgattt tgtaaaaggt gatcaagata ctcgaatcaa tgttaaaatc
    11161 aagtcacaag aagagactga aataaatggt caaggacctg tcattagtcg ggaacaacta
    11221 gaggatttga aaactaagct ttcccagcta acagcagagg atacagttgt ctttgcaggt
    11281 tcagcgccag ctaatcttgg caatgctgtt tataaggaat tatttccttt agttaggcaa
    11341 agcggtgctc aggtggtttg tgattttgaa ggacagactt taatagatgc gcttacatat
    11401 aatcctctgt tggttaaacc taataatcat gaactagaag ctatttttgg tacgattctg
    11461 actagtcttg atgatgttga aacctatgcc cgtcgtcttt tagagatggg tgctcaaaac
    11521 gtcatcattt caatggctgg tgatggtgct ttgctggtta ctaaagaagc gacttatttt
    11581 gctaaaccga ttaagggaga agttaaaaat tctgtcggtg ctggtgattc gatggttgct
    11641 ggctttacag gcgaatttat gaagagtcaa aatccaatag aagccttaaa atggggcgtg
    11701 gcttgtggta cagcgacggc tttctctgat gacttggcga caattgcatt tattaaggaa
    11761 acatatcata aagttgaggt agaaaaacga tgaaaattca agatttattg agaaaagata
    11821 ttatgattct cgatttacag gctatttcta aagaagttgc cattgacgag atgattacta
    11881 aattagttga aaaagatatt gtacatgatt ttgatgtctt taaaaagagt atcatgacac
    11941 gtgaagaaca aacatcaaca gggcttggtg atgggattgc catgcctcat tctaaaaata
    12001 ttgtagtaga taaaccagcg gtcttgtttg cgaaatcaaa taaaggtgtg gattataagg
    12061 ctttagatgg tcaaccaacg gatctcttct ttatgattgc tgcgcctcaa ggagcaaatg
    12121 atactcactt ggctgcgcta gcagaattat cacaatacct tttgaaagac ggctttgctg
    12181 ataaacttcg tgctgcagcg acaccagaag cagtcattgc tgtttttgat gaagcgtcaa
    12241 cagctaaaga agaagtggtt gctccaacaa gtggtcaaga ctttatcgtt gctgttacag
    12301 cttgtccgac aggaattgct cacacttata tggcagaaga ggctttgaaa aaacaagctg
    12361 cagaaatggg agtagctatt aaagttgaaa caaacggtgc ttctggtgtg gctaatcgtt
    12421 taaccgctga agatattcag agagctaaag gagttattgt tgcagctgat aaagcggttg
    12481 aaatggaccg ttttgatggt aaacaattca ttgctcgccc tgttgcagat ggtatcaaga
    12541 aaagtcagga attgatttct ttgattttaa acaatgaagg aaacacttat catgctaaaa
    12601 atggaaaatc tgaaacagca gtatcaactg agaaaacaag tttaggcggt gctttctata
    12661 aacacttgat gggcggtgtc tcacaaatgt tgccatttgt tattggtggc gggattatga
    12721 ttgctctagc atttttattg gataacatgc ttggtgtgcc aaatgatcag cttggaagtc
    12781 ttggatccta tcatgaaata gcagctattt ttatgaacat tggtggagca gccttttcct
    12841 tcatgttacc agtactagca ggttatatcg cttattctat tgctgaaaaa ccgggcttag
    12901 tagctggttt tgtggcaggt gccattgcct caaatggtct tgcttttggt aaggtaccat
    12961 ttgcagcagg aggagaagtg agcttgggat taacgggcgt gccatcaggt ttccttgggg
    13021 cgcttgtggg tggtttcctt gccggtggtg ttattcttgc ccttcgtaaa ttgttggcag
    13081 gcctaccacg ttctctagaa ggggttaaat ctatcctcct ttacccatta cttggcgtcc
    13141 ttgtcactgg tttcttgatg ctttttgtca atattccaat ggcagctatt aacacagctc
    13201 ttaatgattt cttacaaggt ctttcaggaa gctctgctgt ccttatggga cttcttgttg
    13261 gtggaatgat ggctgttgac atgggtggtc ctgttaataa ggcagcttat gtttttggta
    13321 caggaacttt agcagctact gtggcaaatg gtggttcggt cgttatggcc gctgttatgg
    13381 caggtggtat ggtacctcct cttgctgttt ttgtagccac tctcttgttt aaagataaat
    13441 ttaccaaaga agaacgcgaa tcaggtttga ccaacattgt catgggactt tctttcatca
    13501 ctgaaggagc aatcccattt ggcgcagctg acccagcgcg tgcaattcct agctttattg
    13561 ctggttctgc cttgacagga gctcttgttg gtttggctgg cattaaatta atggcgccac
    13621 atggtggtat cttcgtaatc gctttaacaa gcaatccaat cttatatctt gtctttgttg
    13681 tgattggtgc ccttgtatca ggtatcttat ttggcgctct tcgcaaaaaa gcctaaaagg
    13741 taaagaactt ttttcttact aaaactagaa tctgtcaagg ttctagtttt ttgctatact
    13801 agagttaaag gataagaaat gaggaccttt gaaggtgaaa aaaaggagaa gaagggctaa
    13861 atcatcagta aatcgacttg tcttagggtt agtattactt aatctgattg ttagtatgtg
    13921 gacactaaaa ttgggaaacc agcgattagc tccgtatgcg gatcacgaaa ctctgacatt
    13981 tgtcaggaag attagtcatg ctgctcaatc agtcgctcaa aagaaacagt tatacagttc
    14041 ggtgatgatg gctcaggcca ttttagaatc caataatggc aagtcacaac taagtcaaaa
    14101 accttattat aattttttcg ggattaaggg gagctataaa gaacggtcag tcatttttcc
    14161 aactttagaa gacgatggac aagggaatct ttatcaaatt gatgctgctt ttcgttccta
    14221 tgggagcctg acagcttgtt ttttagatta cgcaagagtt ttgaatgatc cactttatga
    14281 caaaacccac aaaaagtttt ggtctcatta tcaagatgct actgcaacct taacaggcac
    14341 ttacgccact gatacaactt acaataccaa attaaatgaa ttgattgaat ggtatcaact
    14401 cactaatttt gatggtctaa tgaaatagtt atccacaatt ttaaaaaaaa tcagaagaaa
    14461 tatgtcgatt tcaagacaaa agttatcaat ctcattaaat tatctgtgga aaagttaatt
    14521 taaacgttcg tgtgctataa tagaaacata gaaaatgtat aaaaaaggat atgtgaataa
    14581 tgacaaaaaa gaaaggtaag cttgtactta tcagtctttt tgttctagca gcttgtttag
    14641 gagcttatag tgcaatgaga caatctcata aaacttcaaa cgtgtcagct gagactatcg
    14701 ccagttcttc aacccgacat tttattgatg aaattggtcc aactgctagt actattggtc
    14761 aagaacgtga tctttatgcg tctgttatga tagcacaagc gattttagaa tcaagtaatg
    14821 gtaaatcaag tctaagtcaa gcaccttatt ataatttttt cggtattaag ggtgcctata
    14881 acggttcttc tgtgacgatg tcaacttggg aagatgatgg taatggcaat acctatacga
    14941 ttgatcaagc ctttagagct tatccaagta ttgcagactc ccttaatgac tatgcagact
    15001 tgctaagttc tagcacttat ataggtgcta gaaaatcaaa cacgctatcg tatcaagatg
    15061 ctacagcagc tctaactggc ttatacgcta cagacaccag ttataactta aaactaaata
    15121 atattattgc aacctatggt ttgacggctt atgatgttgc taatagctca gctcaagaaa
    15181 ctggtctagc aacttcgggt tatgtttgga atgagtatcg tcgtaattac actgatgcgg
    15241 agaccctagc agttgatgag gcttgggcta aacgcatgac ttattaatgt aacactaaca
    15301 acaatctgct tgcactgacc ccaaaaaagc tggatactct attttaagta aaggatttag
    15361 ttctgtattg tacagaacta aatcctttta gttttacttt gatatgttta tcgttgtaat
    15421 aatcaatgtc atttgtaatc gcttattctg agtcttctag tgatttacag ttattctcat
    15481 aaccatagaa catttctgat tttaagatgc caaagaaaga ttccatcatg ccattgtcag
    15541 gcctgtttct tttacgtgac atagacggac gaattccttt agagtttagg aaatgatgat
    15601 aatagttgtg ttgatattgc caaccttgat cactctatca tatagttccg tcatagcatt
    15661 tatttggaga tgcctttttc cattattggt ttaatgtgat ttaagcaagg ttattagcta
    15721 aacttaatgc cagacaaaaa caccccaatt attagatttt ttgtctaact tttggggcgc
    15781 aattcaatct gagcagtttt agatttggta tcaatgttat tataggtcaa ttgatcatat
    15841 ttatccagag cttttagctt atttagtcca aagataaata tcaaaatggc tgaaatcttt
    15901 catatttata gtcttattgt ctttatattt tttaaaaata tcactccttg tactagatga
    15961 ggttgcatcg tataagttaa aactttcatg tttattgcca ttgatcgcaa tttctaattg
    16021 cccttttatg tatggagaat taggatcata aattttgtat gtttgcataa gatattgtct
    16081 gattttgaag tcaaattctt gaatcgtaaa aatacgttta tctattttta tgggatttat
    16141 tagtgttttt tgctggactc catctattag taaattacct acaattttac t
//
LOCUS       DATUYR010000034        15519 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.034, whole genome shotgun sequence.
ACCESSION   DATUYR010000034 DATUYR010000000
VERSION     DATUYR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15519
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.034"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(13..2004)
                     /gene="uvrB"
                     /locus_tag="VCI33_001101"
     CDS             complement(13..2004)
                     /gene="uvrB"
                     /locus_tag="VCI33_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEP2832030.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIIYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            2196..4370
                     /locus_tag="VCI33_001102"
     CDS             2196..4370
                     /locus_tag="VCI33_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP2832031.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDIVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            4370..5110
                     /locus_tag="VCI33_001103"
     CDS             4370..5110
                     /locus_tag="VCI33_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2832032.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVVVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEAAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(5258..5410)
                     /locus_tag="VCI33_001104"
     CDS             complement(5258..5410)
                     /locus_tag="VCI33_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832033.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(5407..6786)
                     /locus_tag="VCI33_001105"
     CDS             complement(5407..6786)
                     /locus_tag="VCI33_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP2832034.1"
                     /translation="MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGIFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(6964..7413)
                     /locus_tag="VCI33_001106"
     CDS             complement(6964..7413)
                     /locus_tag="VCI33_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEP2832035.1"
                     /translation="MIISAEGDVSVHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANYFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(7410..7745)
                     /locus_tag="VCI33_001107"
     CDS             complement(7410..7745)
                     /locus_tag="VCI33_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP2832036.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(7748..8059)
                     /locus_tag="VCI33_001108"
     CDS             complement(7748..8059)
                     /locus_tag="VCI33_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP2832037.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(8082..10076)
                     /locus_tag="VCI33_001109"
     CDS             complement(8082..10076)
                     /locus_tag="VCI33_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP2832038.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVTQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTTRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVAICREPLEWDKAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            complement(10182..11276)
                     /locus_tag="VCI33_001110"
     CDS             complement(10182..11276)
                     /locus_tag="VCI33_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEP2832039.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAEQSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEKIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGSIIIDNDRYHRYKGELQIALKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            complement(11285..12685)
                     /locus_tag="VCI33_001111"
     CDS             complement(11285..12685)
                     /locus_tag="VCI33_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP2832040.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSDAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEVIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            12910..13605
                     /locus_tag="VCI33_001112"
     CDS             12910..13605
                     /locus_tag="VCI33_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEP2832041.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            complement(13610..13768)
                     /locus_tag="VCI33_001113"
     CDS             complement(13610..13768)
                     /locus_tag="VCI33_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832042.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(13837..15150)
                     /gene="obgE"
                     /locus_tag="VCI33_001114"
     CDS             complement(13837..15150)
                     /gene="obgE"
                     /locus_tag="VCI33_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEP2832043.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVNEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDESDLVDEEAYYGFAEAEKEFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDLVRIGKFEFEFVD"
     gene            complement(15207..15335)
                     /locus_tag="VCI33_001115"
     CDS             complement(15207..15335)
                     /locus_tag="VCI33_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEP2832044.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIASALSVLM"
BASE COUNT         4662 a         3324 c         2790 g         4743 t
ORIGIN      
        1 tttattcaaa acctagtcca ttaatttaag ttctaaaatc aagtctcgca tttgagctgc
       61 caattcgaaa tcaagtaatt cagctgcctc ttgcatttgt ttttgcagag cattgatcgc
      121 ttcttttcgt tctcctcttg acatactttc ataatccatc tcttctttcg atatatcatt
      181 gtggcttgtt ttacttatac taataaggcc tctgatgtct ttttttatag tttgtggaac
      241 gatgccgtgc gctttgttat atgctatttg aatctcacga cgacgagccg tttcgtcaat
      301 agcccgctgc atagaatctg tcatcttgtc cgcatacata atgacatgac catctacgtt
      361 tcgagctgct ctaccgatgg tctgaatcaa gccgcgttca ttacggagga aaccctcttt
      421 atcagcatcc aaaatggcta caagactaac ttctggcaca tcaatccctt cacggagcag
      481 attgattcca atcaagacat caaaaacacc aagtcgtaaa tcacgaataa tttctgtccg
      541 ctctaaggtt ttaatatcac tgtgcatata cttgaccttg acacccatct cttttagata
      601 atcagtcaaa tcttctgcca tttttttagt taaggttgtg atgaacgtcc gttcatcgcg
      661 cgctacgcgc tggttaattt ctcccaacaa atcatccatt tgccccatac ttggccttac
      721 atcaatttca ggatcaagta gcccagtcgg acgaataatt tgctcaataa ttgtattagt
      781 ctgtgacatt tcgtattcac caggcgttgc tgaaacatag ataatttggt gcacatgact
      841 ttcaaattcc tcgcgtctta agggacgatt atcaagagct gatggcaatc gaaaaccata
      901 gtcaactagc atttgttttc tggcttggtc accattatac atgcctttga tttgtcccat
      961 tgtcatgtga ctttcatcaa tcataattaa aaaatcttct gggaagaaat ctaacaaggt
     1021 ataaggaggt tctcctggtg acctgccatc catatgacgg gaatagtttt cgactccgct
     1081 agtatagccc atttcacgta acatttcaat atcatattcc gttctttggc gtaatcgctg
     1141 tgcttctagt agtttacctt cagattcaaa taactgtaac tgctctgcta actctgcttg
     1201 aattttggct attgattgct ccatatgctc atcatttgtt acaaagtgag tagctggaaa
     1261 taaaaccaaa tggtcaactt ctccaatagt tttcccagtt aaactttcga tttcacatat
     1321 acgatcaatc tcatccccga aaaattccac ccgaaaagca tgttcatccc tggatgctgg
     1381 aaagacctca acaacatcgc cacgtacacg aaaacagccg cgttgaaaat caatatcatt
     1441 acgctcaaat tgaatatcta ctaattgatt tagcaaagtg tcacgtgata tctcctgacc
     1501 tggacgtagg ctgactgctg aatctgcgta ctcttttggc gaacctagtc cataaataca
     1561 agatacagaa gctaccacta tgacatcgtt acgctcaagt aaagaagagg tggctgaatg
     1621 gcgaagttta tcgatttcat cattgactga actatctttt tcgatatagg tgtcgcttga
     1681 tgggacatat gcttcaggtt ggtagtaatc ataataggaa acaaagtatt ccacagcatt
     1741 gtctggaaaa aattctttaa attcaccata taattgtcct gctaaggttt tattgtgagc
     1801 tatcactaag gtaggtttat ttactttact aattacttgg ctcatggtgt atgtttttcc
     1861 cgttcctgta gcacctagga gaatctgagc tttttctccg ccctcaatat tatcaactag
     1921 gctttcaata gcttgtggct gatcaccaga cggtttatac tttgatttta gtttaaatgg
     1981 tttatcatca cgtttatcaa tcattttata ccctctaata gtcttatctc ttatagacta
     2041 ttttaacata gataagccag aatcacctcc aataagcgta aaaaacatgt catttacatg
     2101 ttatgtttta tgacatataa ataacaaatt tcttgcttaa tgttcaacat tttgttataa
     2161 ttaaccatat tttttagttt tataaggaga ttagtatgac gcacaaaata aaagtattgc
     2221 tgcttgcgat aatgtctatt tttttgacat gcaatattgc aagtgctgaa actattgcta
     2281 ttgtttcaga tacagcttat gccccatttg aatttaaaga ctcagatcaa atttacaaag
     2341 gaattgacgt tgatattatt aatgaagtag ccaaacgtca atcttgggat ttcagtatga
     2401 gtttcccggg ttttgatgca gctgtaaatg ctgtacaatc tggtcaagcg agtgctctaa
     2461 tggccggtac aaccattacg aatgctcgta agaaagtctt tcatttctca gagccatatt
     2521 acgataccaa aattgtcatt gcgacacgta aagccaatgc catcaaaaaa tacagtgact
     2581 taaaaggaaa aacggtcggt gttaaaaatg gaacagcggc tcaagccttt ttgaataact
     2641 ataaaaaaaa gtatgattat actgttaaaa catttgacac aggtgatctt atgtataata
     2701 gtttatctgc tggttctatt gccgctgtta tggatgatga agcggttatc caatacgcaa
     2761 tcagccaaaa ccaagatatt gctattaaca tgaaaggaga gcccattgga agctttgggt
     2821 ttgctgtcaa aaagggaagc ggatatgatt atctagttaa tgatttcaat acagctctta
     2881 aagctatgaa agctgatggt acctaccaag ctatcatgac caagtggtta ggcacagatg
     2941 ataaagctac caccagtcag gcaacgggaa atccatctgc caaagctaca cctataaagg
     3001 acagttataa aattgtctct gattcgtctt ttgcaccttt tgaatttcaa aatggtaagg
     3061 gcaaatacgt tggtattgac atagaattaa tcaaagctat tgctaaacaa caaggtttca
     3121 aaattgaaat cgctaatcca ggtttcgatg ctgccttaaa tgctgtgcaa tctagccaag
     3181 cagatggggt cattgctggt gcaactatta ctgacgctcg taaagctatc tttgattttt
     3241 ctgatcctta ttatacttct aatatcattt tagctgttaa agctggaaaa aacatcaaga
     3301 actatgaaga cttagacaga aaaacagtcg gtgctaaaaa cggcacttca tcttactctt
     3361 ggttaaaaga aaacgctcct aaatatggtt ataatgtcaa ggcatttgat gatggttcta
     3421 gcatgtatga tagcttaaat tcaggttctg tagatgctat catggatgat gaggcggttc
     3481 ttaaatacgc tatttctcaa ggtcgtcgct ttgaaacacc tcttgagggc atttctactg
     3541 gtgaagttgg ttttgctgtc aagaaaggaa ctaatccaga attaatcgaa atgttcaaca
     3601 atggcttagc tgctctcaaa aaatctggtc agtatgatga cattatagat aaataccttg
     3661 actctaagaa agctgcaact ccttctgaaa aaggtgctga tgagtctact atttcaggcc
     3721 tattatcaaa taactacaaa caactattgg caggacttgg aaccacgctc agtttaaccc
     3781 ttatttcatt tgctattgct ataattatcg ggatcatctt tgggatgatg gccgtgtcac
     3841 caactaaatc acttcgactc atttcaacgg tctttgtaga cattgttcga gggattcctt
     3901 tgatgattgt ggctgccttc attttctggg gagtaccaaa ccttatcgag agtatgaccg
     3961 gccaccagtc accgattaat gatttcttag ctgctacaat tgcactgtca cttaatggcg
     4021 gagcctatat tgctgaaatt gttcgcggtg gtatcgaagc tgttccagca gggcaaatgg
     4081 aagctagtcg aagtcttggt ttgtcttacg gaaccacgat gagaaaagta attctcccac
     4141 aagctgtgaa actaatgtta cctaacttta tcaatcagtt tgttatttca ttgaaggata
     4201 caacaatcgt ctcagcaatt ggtttagtgg aactcttcca aacaggtaaa atcattattg
     4261 ctagaaatta ccagtcgttc cgtatgtatg ctattttagc aattatttac cttatcatga
     4321 ttacactctt aacaagactt gcaaaacgtt tagaaaagag gcttaactaa tgacagaatt
     4381 aaaaattgat gttcaagatt tacacaaatc ttatggtcaa aatgaagtct taaaaggaat
     4441 tgatgccaaa ttctacgaag gtgatgtcgt ttgtatcatt ggtccttctg gttctgggaa
     4501 atcaaccttt ctacgtaccc tcaaccttct agagacgatt accagtggta aggtcgtggt
     4561 ggacggtttt gaattatcag atcctaagac taatattgac aaggcacgtg aaaatatcgg
     4621 gatggtattc caacacttca accttttccc tcacatgact gttcttgaga atattatctt
     4681 tgctcctgtt gaattaggta aagagtctaa agaagctgct aaaaagcacg gcatggcact
     4741 tctagaaaaa gtcggacttt ctgataaggc cgacgccttc ccaggaagtt tatccggcgg
     4801 gcaaaaacag cgtgtcgcca tcgccagaag tttagcaatg aacccagata ttatgctctt
     4861 tgatgaacca acttctgctc ttgaccccga aatggttggg gacgttttga acgttatgaa
     4921 agacttggca gaacaaggca tgaccatgtt gattgttact cacgaaatgg ggtttgctcg
     4981 tcaagtagct aaccgcgtta tctttacaga tggtggtcaa tttttggaag atggaactcc
     5041 tgaagaaatt tttgaccatc ctaagcaccc tcgtctcatt gaatttttag acaaggtctt
     5101 aaacgtctaa tagagcagac accatactaa cttccaaaaa ataataaggc aaggaaacag
     5161 gtttccttgc cttttttggt tactgttatt attcaaacca gcaaaatagg gatactctta
     5221 tgactctcta cttcataaga gtgtccccta tgctttttta tgggcgttta tgtttagtga
     5281 ggtaataacc taatcccgta agagctagtc ccgtcactat gtgtaagtat ttgttaccaa
     5341 ccatcggact aatggcaatg agaaaaaagc caacagccat taaaaaagtt gctccatttt
     5401 tttctgtcat gcaaggtcac tcgctttttc tttattggcc aaaatgacaa atggtgccca
     5461 gataaggaag gcaacaaaga ggttaaacag tgaaaccaaa cctgccataa tattacctcc
     5521 agtagctagg taagcataaa gtccaacagg ggtaatccaa ggtactgata agaataccgg
     5581 tggaatcaaa cctattgcag tagcaaaata agctatgcta gcacaaattg gtggcacaat
     5641 tagccatgga atcacaaagg ttggattcaa aacaattggc atcccaaatg taattggttc
     5701 gttgatattg aaaataccca ttggtgcgga caatttggca atggttttat gctcttctcg
     5761 ttttgagaat atgaagatag caataatcaa tgccaaggtt actcctgaac cacccatttg
     5821 agcataagca tcaaaggacc cgcgtgtcca aaggtatggt aaattagcag cgctatgcgc
     5881 tgtattgtaa gcagctgtat tttctgttaa agctaccata taaataccat ccatcactgg
     5941 ggctaagaca ttatgaccat gcaagccgaa gaaccagagc agttgaacta agaaggttaa
     6001 caagataact gaaccaatac cctgtgataa gcctagcaat ggtaattgga tataggttga
     6061 aatgacatca cttagggaag aacctgttaa tgcgaagatg aggtaggcga aaattgccga
     6121 agcgtaaatc gcaactgttc ctggaataat ggcagcaaag gctttattaa ccgcaggtgg
     6181 tacattatct ggtaaggtaa tggtaatatt acgtttggtc aaagccgcat acacaaaact
     6241 tgataaaaag ccaataatta aagctgtgaa caagcctgtg gcaccgacat actttagagc
     6301 tattgctccc cattgactag tttcaatact tgaagctcca cccgcagtaa cgactgaaag
     6361 ccccatttct ttcaaattgc taattaggtt agcagaggca ttttcaagtg gtgtcgaaat
     6421 ggttaaagtc tgaggtaggg tggcaataaa tgagccaaaa gaaatcaatc ctcccgccaa
     6481 tggattaatc ttatgcataa cagctaaatt gtaaccgaaa gcaaaggcaa aaaagagcgc
     6541 catgatacca attgtcccaa aataaacata gccattgatg tcgataacag gttgcatagc
     6601 ttttgcaaac cctgtccatc ccatattgtt tggaatatcg cgtaaaaaaa cgttcaataa
     6661 gacagcaact gaacctgcca tggtaatagg catcattgaa ataaaggaat cacgaatagc
     6721 tactaaatgc cgttgtgacc caatttttcc tgaaatctcc ataaacttat cagaaaaatg
     6781 cgacataaat cttctcctct ttttattttt ttaatgtaac aaaggtaacc ttaaaagcag
     6841 cggtgtctgc ctaacacaat tatctcaaaa tcatccttgt taaacgtgat gcttagagat
     6901 aaccggtaat cggctcttat ccaaatgaac ccttctctct cattcaaggt ctatgaataa
     6961 tcttcacaaa gacgatggtt gctgcaactg tttagcatat tggataaggg ctgctaactg
     7021 ggcttgagat tggcgttcta ggcttttttt gaacagtttg ccaacaataa aataatttgc
     7081 tttttgaaaa atatctagag gatgatagtc ataagaatag gtaattctag tccgattctc
     7141 ccccagagcc tctaatcgat aagccaattc ctttatccca tgattggaac taaaacaagc
     7201 agtatataat tgcggagctt ctagggccgt aataagcacc ctcactgtac gctctttgta
     7261 tttaccataa tgtttaacat agtttatccc cgctttgata tcatctcttg acaactgtct
     7321 caaggtattt tgtcgataat cttcttggag cgatatcagc atggcttcaa aacaatgatg
     7381 gacggaaaca tctccttctg ctgaaatgat catgactcac ctctttgtat tgctgacatt
     7441 ttgtcgtaaa cggtaaccaa ttccttggct aaatcaataa aggctaaggc agtcatcatg
     7501 tgatcttgcc catgcaccat caataaagac aattcaaccg catcaccagc tgcttcttgg
     7561 gacagcaaat gtgtctgcga tttatgggca atatttaacg agtttgtcgc ttcttgaagt
     7621 aagtgctgag cctcctcaaa ctgcccatct cgtgctgcat gaatagcctc tacggccttg
     7681 cccttggctt cgcctccata catgattaag cccataatag cttctaagtt ggatggttcc
     7741 gtcatgccta ctcctccatc atcgctaggg ctgtttcaag gactttttca ccgttcataa
     7801 gaccataatc agccatgtta atggcatcta ctttaatatc tggttcaaac ttatctttaa
     7861 aatcctttag taagaatttg acttgtggac ctaacaagag aacgtcaatg ctattagcag
     7921 ccacttcgtt gtcagcttca ctcacaggaa ctgcccaaat agaaacttca aggtcacgcg
     7981 cttgggctgc tttttgcatc ttagtcacca acatactagt tgacataccg gcattacaaa
     8041 ctaacataat agatttcata aggtttctcc ttttaatcgt tttaatcatg aataagtggg
     8101 gtaaacactg tcatgaattt ggaataattt ggttcttcta gtaacgcttg ctgaagctcc
     8161 acctgattga caaaagatgc caaacctggt gatacatatt ttaacctatg attatgccct
     8221 ttagacgggg ataatagaaa gacaaaatga acagcttgat gcgccttgtc ccactcaaga
     8281 ggctctcggc aaatagccac tactacctgt tcagaataag tcatgggatt agcaggatgc
     8341 ggaaaggcaa gcacttctcc atagatgact gggctgtaat tttccctcag taccatttga
     8401 tgataaaagt cctctacaaa cgtttctgtt cccgcctcgt caagacaagc aatcatccgt
     8461 aaaagcaagt cttcctttga gattttctcg tctaaataaa gaaaacgatt agggctaaaa
     8521 acacctgtta gaagctgctc tgcttttgct aaactcatct gtgacggtaa actgacctct
     8581 ttcttgatac cttcttgttc tccaataaat tgtcgaatag tttcaatatc ctgatttgac
     8641 aaaaaaacac tgacagtaat cactggtgtt aaaaacatca gattggcaag gctgatagag
     8701 gaaataatca aatcaacggt tttcagtcgc tcttcggtta tttcatagta actgataaca
     8761 tcgacaatac gcaaacgtcc ttcaaattcc ttttccaagc gatttttcaa catcatggca
     8821 ctaccataac ctgtggcaca aactactaga accctcaatt tatgacggtt tgaataacgc
     8881 tcaatggccg ccattaaatg caaactaata taagcccatt catcatctga gacatcattc
     8941 ttttgacata caagcaaatc cgaaaaggtc tgcttggtaa gggtaaatat ttcaggatac
     9001 tggcttttaa tttcctcagt taaaggatta gtcagctgaa tatggttttc aagacgtgtg
     9061 gtcaagggca tcatgtgggc taataaacct tgaaataaat tggtatctgt ttctaaagtc
     9121 atgcctgtca attgactaat cttggcaata catgctttta aatggtcctg caacttttca
     9181 tcggtattat catcttgcca atgcttacca acagaatgtt tgacctttag atgcagtgca
     9241 atataattag cctcctcttt aggaaaacgt atccccatca cttcttccac tcgatacaaa
     9301 atgcgcagag ctacttgata ctcatcagac tgacgaatag ctattggaat ggaaaacagt
     9361 tctaatggac accccgatcg aatacgctga accattaggg caatatgcaa gactagatta
     9421 ttcatgacaa aatcagaaag tttcaactta gcatcccgac attcatccaa gacaataatc
     9481 atgatttctg caaaattaat accttcaaga aaggtattac ctaccatggt ggacaaggtg
     9541 ttatcaaaac tagtaacaaa aaaataatcc atgataaaat gcctaatgtc ctcttcttta
     9601 cctcttattt gaatatgttg gtgagttatt tcaagctcta actggtaagg tattatgcgc
     9661 tccttaatgc ctgctaaaac atgttttaaa gttgtccgac tgatatacaa ttcttgacaa
     9721 agctcttcaa aatcctgaac ggcatcttca aaaaagaatt tatgaagcac ataatcttct
     9781 cgatcaaccg attcttccac ttgagtcaca tctgctaaac gttttttaga cgtcacactt
     9841 tcttgccaaa agatgtcaaa tgccatagag tggtcaattt ctaagcagta acctcgacct
     9901 tgtttagaaa tgatgtgagc tccgtgttca ggaaggctac tgatcagttc tttgaggtat
     9961 ttcctaaccg tacggtcact cataccaata acctcagcca gagttgcact agagacaaac
    10021 tgctctttgt gatggattag aaatgttatg atggccagtt ctttttttga taacattagt
    10081 cctccttact actttagaaa acgctctcta ccctctttta gtgtaccaaa tatttcctga
    10141 aaaaggtgac catcattttc cgcattgatt gtgaaaagaa tctatgatgg gtcaatctcc
    10201 aaacaaaatt cctgccaagg tcgcaaatca tctaacaaac taagatagtc ctctctgacc
    10261 tcagcaacca cattagcttt tgaactaacc gtaaaatttt tcaaagcaat ttgcagttct
    10321 cccttatatc gatgatagcg gtcattgtca ataataatgc tacctcgttt gacctcctta
    10381 gattggtctc ttggagcaat cgattcctga gcataaacta gacgtggcat ggttgatcga
    10441 atcacataat cagatacatc gccacgataa caatgcggat agccaatgat tttctcctca
    10501 acttctgtca aatcgatgat aggcctaacc ttgatcgttg tgactggtct tgctaaagcc
    10561 tgagtacatg ctgccaattc ttcttccgaa atgaattggt tagatatgag gatattatca
    10621 atggttccta tcgctttcat taattctact tgcaggccaa tgggcaagtg cctatggtct
    10681 tccactgtcg gcaagccttc tgccagtagc caagggcctt cagatgctga ctgagcggta
    10741 ataaaagcag ccgtttcaat atcatgctca tgataaaaac gtgacatatc cttaaaatgt
    10801 tgccatgaca aaccggtaaa ttcatgtgga taaaaattat ggcaaccaat aatattgctc
    10861 tgttctgcat ctgtcgctag caatgacatc aataattgtt tatcagtgct catattaagt
    10921 tcaattttaa gcccaaaggg atttcttgtt aaagttacaa tctctgctag agggagagcc
    10981 tcgtctaagc gtaagcccgc taacccgaag gcatgagctc gttcaataag ctgatctgac
    11041 caaccagctt ggctaataaa gcttggacta acgtcagcaa ttactcgaat gcctaattga
    11101 tttgcataag caatgagctc tgcgtagcaa tgaaacatct ggtgatcagc tggtgccagc
    11161 tgtaagagac tcataaacaa gcgcttggct ccatagctat gacataagtc aatataagct
    11221 ttactcttgg taacatcata ccgctcaggg tatagagaaa atcctaaatc aaccatattg
    11281 cctcctattt atcaaaacca tttgactcgc tcaacgcctt aaaccagtaa cctgattttt
    11341 ttatggttcg cttttgactc tctaaatcca aagctaccaa accatatcgg tttttataag
    11401 catttaacca agaccagcaa tcaataaaag tccacaagag gtagcctttg cagttggctc
    11461 cttctcccaa agccttatga agctggataa gatgatcctc gataaaggtg atgcggtagt
    11521 catcttgtat ctggccatct gctaaaaaag cttcttcacc ttcgacaccc ataccatttt
    11581 ccgtgactag ccattcgatg ttaccatagt gttcttttaa attaagggca atatcataaa
    11641 gtcctggttc ataaatttcc caaccccggt gaggattgac ttttttacca ggcatatcat
    11701 aaggttcaaa ataaatatct aagataaggg ggtccccctc ttgctgtgac ttactaggtg
    11761 cctgaacccg caaaggctga taataattaa ctcccaaaaa atcaacagta ttgtttttga
    11821 taacctctaa ttcatcagca ctatattgag gtaataaatc agcttcctct aaaatggaaa
    11881 ctaactctgc tgggtaaaca cccaaaactg atggatctag gaaggatttg gtttggaaaa
    11941 gctctgcgat tctagcagcc ttgacatctt caggagcatc actccgagga taagcaggtg
    12001 tcatgttaag cacaatacta atcttgtggt cgggatgaag cttatgacaa gctttaaccg
    12061 ccagtgaact ggccagttgt gtgttgtaag ccacttgaac tgctgccttg gcatctactt
    12121 tacaagggta gtgataataa cctaaatagc cacattcgac tggaacaatc ggttcattaa
    12181 aggtaatcca agtgtttact aaatcaccaa aaagctcaaa acaggttttt gcgtaggttt
    12241 cataagccca gacagtagct ttgttttccc agccaccttt ttcttgcaag gcatagggga
    12301 ggtcaaaatg atagaggtta acaatcaatt taataccttg agcaataatg tcttgaaata
    12361 cttctcggta aaatgtcacc gcctttggat taacctcccc gactccttct ggaatcaatc
    12421 gagaccactg gattgaggtc cgaaaaatcg tgtggcctgt ttctttcaaa agggcaatgt
    12481 cacccttata gttttcataa aaggttgagg ttttttctgg accaatttgg tgatgaaact
    12541 tagctgactc aatactaaac cagtaatccc agttgctagg tcctttgcca tctccaggaa
    12601 ccgttccttc gctttgaggc ccagatgtgg aacttcccca taaaaagcca tctggaaatt
    12661 gataacggtg ttttctttgt ttcatatcct ttgtcctttc tttgctcact tctcttttta
    12721 ttataagaag aaaaacaggt gatgtaaacg ctttaaaaaa tctgtttttt ccttatcatc
    12781 acggaaaata agtgatttaa agcttgacat attttattat cttaaagagc ctatctaacc
    12841 tgccctgcta cttcttatca aaaccaagcc cctattgaca gatgccttag cctatgtttg
    12901 aataggggta tggcttattt aaactacttt acaaaaaccg agtgggcact ttggttaagc
    12961 tctgtcttgg ccattttaat tagcgccctt ttattcggta agcaagctcc cttagcttta
    13021 atagcttctt tgattggtgt gacctctctc atttttagtg ctaaagctaa tcctattggt
    13081 caagggctcg tcattatttt ttccattatc tatgcctatc tttcattacg aaacagttac
    13141 tatggagagt taatgaccta tcttttcatg accctcccta tgactatttt ttcacttttt
    13201 acttggttaa accatccttt tgaagggaaa aaatcacaag tgactatttc acgattaaca
    13261 cccactgatc gaagatgtct tttcgttttt actatactgg tcactcttat tttctacggc
    13321 atcttggctt ctattcaaac agcctattta cttgtctcca ctttatcgat tgccacttct
    13381 ttctcagccg tttatctcag ctataagcga agcccttatt ttgctttggc ttatggactc
    13441 aatgatctag tcctcattct cttatggatg catgctgctc aaacagatgc cagccaatat
    13501 gctgtggtca tctgctttgc cacctttctt attaatgata gttacacttt ttatagttgg
    13561 ctaatgctcc aacgttacca agaaaaaaag gtcaaaaaag gatgagggtc taagcctcat
    13621 cctttttcaa tttttcacgt tctaagcgca aacgtctatt gggatttaaa agattaaaca
    13681 attcttctaa tttctcagca ttccaaacat ctgctgccga tacaaaattt ccatccttat
    13741 ccttaaagga tatcggttta tcacccataa tcagaaacct ccatcttaaa gataaaacac
    13801 aaggctgcta tggcaatgcc atttctaaag accacattaa tccacaaatt caaattcaaa
    13861 tttgccaata cgaacaagat ccccgtcttt tgctccacgt tcacgaaggg cttcgtcaac
    13921 ccccataccg cgcaattgac gggcaaattt catgatggat tcgtcacgtt ccatattggt
    13981 catgacaaag agacgttcta acttctcacc agacaagacc caagtagcgt catcgtcacg
    14041 tgtgatctca aattctttct cagcctcagc aaaaccatag taagcttcct cgtccaccag
    14101 atcagattca tcatacaata agaattcatc cgtcttagca agtaattctg ccgttgcttc
    14161 taacaagttc tctaagcctt gatgtgctag gcttgaaata gggaaaatca ttggcaaatc
    14221 atcaaactcg tcatattggg cagccaattt tttcttgaag gctttcaagt tttcttgagc
    14281 ctctggcatg tccattttat tagccacaat aatctgtggg cgctccatca atcgcaaatt
    14341 ataggtttcc agctcgttat taatagaaac ataatcttcg taaggatcac gaccctcact
    14401 agcagacatg tcaatcacat gaagaataac ccgtgtccgc tcgatatgac ggaggaattg
    14461 agttcccaaa ccaacccctt ggctagcacc ttcaatcaaa cctggcagat ctgccatagc
    14521 aaagctatca cctgactttg tacgaaccat tccaagatta ggaacaatag tggtaaaatg
    14581 ataggcacca attttcggtt tagctgatga cacaacactt aacaaggttg atttcccaac
    14641 agatgggaat ccgaccaagc caacgtcagc taagattttg agttctaact ctaactgacg
    14701 ttcctcacct ggctcaccat tttcagcaat ctctggcgca ggattacgag gagttgcaaa
    14761 acggatattc ccacggccac cacgaccacc cttagcaatg accacttctt gaccatgttc
    14821 tactaagtct gtaattactt ttccagtttc agcatcacga accgttgtcc cttgaggaac
    14881 aaaaacaatc aaatcttctg caccacggcc gtgcatgcct ttggtcatac ctttttcacc
    14941 agatttcgcc ttgaatttac gattatagcg gaaatccatc aaggtacgca aaccttcgtt
    15001 cactcggaag ataactgagc cacctttacc gccgtctcct ccccaaggac cgccatttgg
    15061 gacgtacttt tcacgacgaa aagctaccat accatcgcca ccacggcctg cttggacact
    15121 gatttttgcc gtatctaaaa acatactcat acttttttta ttctctaact ttcttttact
    15181 ttaaaaaaac gcctttcagc gtttccttac attaatactg atagggcact cgcaataagc
    15241 ccgccaacag ttacaagaac cattaggata acaacgacca ttgtcacttt ttcaaaggtt
    15301 gtttttttgc gaggtccatt ttctccaaat gccacgttaa atacctcatc tattgatttt
    15361 tcttcttata tcttaacatg aaagatcgtt ttaggcaaat accagatgaa tatttacgct
    15421 atgtaggcta ttactgagta ttgtatcttg tgaatttcat atgaatatga tacattagca
    15481 tttgcaaaat aatgttacag ataaaaacct cctcgtctg
//
LOCUS       DATUYR010000035        14924 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.035, whole genome shotgun sequence.
ACCESSION   DATUYR010000035 DATUYR010000000
VERSION     DATUYR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.035"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            25..729
                     /locus_tag="VCI33_001116"
     CDS             25..729
                     /locus_tag="VCI33_001116"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEP2832045.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVIAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            853..4578
                     /locus_tag="VCI33_001117"
     CDS             853..4578
                     /locus_tag="VCI33_001117"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEP2832046.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIESLPGQFDQRAVSSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYKADDFTAYKVEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRAGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNDETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEARITSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNAYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKILSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQTQHKIT
                     AASAVKYGGVLESFALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQTDVLEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEVAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            4739..6193
                     /locus_tag="VCI33_001118"
     CDS             4739..6193
                     /locus_tag="VCI33_001118"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEP2832047.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDRGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6221..7243
                     /locus_tag="VCI33_001119"
     CDS             6221..7243
                     /locus_tag="VCI33_001119"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEP2832048.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELINGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            7411..7965
                     /gene="purN"
                     /locus_tag="VCI33_001120"
     CDS             7411..7965
                     /gene="purN"
                     /locus_tag="VCI33_001120"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEP2832049.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLTGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8149..9696
                     /gene="purH"
                     /locus_tag="VCI33_001121"
     CDS             8149..9696
                     /gene="purH"
                     /locus_tag="VCI33_001121"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEP2832050.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVVLKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(9754..10878)
                     /locus_tag="VCI33_001122"
     CDS             complement(9754..10878)
                     /locus_tag="VCI33_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEP2832051.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFTVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            11131..12396
                     /gene="purD"
                     /locus_tag="VCI33_001123"
     CDS             11131..12396
                     /gene="purD"
                     /locus_tag="VCI33_001123"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEP2832052.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGVPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVRPVLEGMVAEGRPYLGVLYVGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFVENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIRE"
     gene            12674..13165
                     /gene="purE"
                     /locus_tag="VCI33_001124"
     CDS             12674..13165
                     /gene="purE"
                     /locus_tag="VCI33_001124"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEP2832053.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            13152..14225
                     /gene="purK"
                     /locus_tag="VCI33_001125"
     CDS             13152..14225
                     /gene="purK"
                     /locus_tag="VCI33_001125"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEP2832054.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGYKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENIDADGLDAVVSACQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLGGLDFTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHCNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFSDLPDEVEEF"
     gene            14227..>14924
                     /locus_tag="VCI33_001126"
     CDS             14227..>14924
                     /locus_tag="VCI33_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832055.1"
                     /translation="MYLKYIFKIKDRNLVSDYESLVRNLTINLQKLVIKDRTALIGDK
                     QLPITIYYRGENEKFPTGIVKVESDSVEGLELFDKTIKEIVGNESRFGKFTCIYDGIS
                     TYYSTEIYSELHEIERRTQEFLREAFFLSDKSGELKEYQATIKKMDSSDFSFSKLINE
                     IFKASDITDTFIDHVRACVEFGTVPNGDLLPKSFWDSLDDSLLDDKEQGLEINNLLNE
                     IRSTRNTIAHCKEF"
BASE COUNT         4287 a         2899 c         3502 g         4236 t
ORIGIN      
        1 acagaaagaa aggcgaataa tcaagtgact aaccagctta tttataaagg aaaagcgaaa
       61 gatatttata gtacaaaaga tgaaaatgtg attagaacgg tctataaaga ccaagctact
      121 atgttgaatg gagctcgtaa ggagactatt gatggcaagg gagcactaaa taatcagatt
      181 tcatctctta tttttgagaa attaaataag gctggtgtgg tgacacatta tattgagcaa
      241 atttcaaaaa acgaacaatt gaataaaaaa gttgatatta ttccacttga ggtggtgtta
      301 cggaatgtga tagcaggctc attttcaaaa cgttttggag tggaagaagg acgtgttctt
      361 gaaacgccaa ttgtggaatt ttactacaaa aatgatgatc tgaatgatcc ttttatcaat
      421 gatgagcatg tgaagttttt gggtattgtt aatgatgagg agattgctta tctgaaagga
      481 gagactcgcc gtattaatga actcttgaaa ggttggtttg ctcagattgg gcttaacttg
      541 attgacttca agttggagtt cggatttgat caagaaggta caattatctt agcagatgaa
      601 ttttcgcctg ataactgtcg cctatgggat aagaatggta accatatgga caaggatgtt
      661 tttagaagag accttggcaa cctgacggat gtctatcaag ttgtacttga gaagttgata
      721 gcattgtaac tagaagagga aggaattgaa tccgttcggg aaactccgaa aaaaagatgg
      781 aacttactga aatctcaatt tcagatgtcc tcctattttc cagtcgctcc cttgtcggac
      841 ttggtatctt atatgaataa acgtattttt gttgagaaaa aggctgactt tggtattaaa
      901 tcggctagcc ttgtgaaaga gttgacgcat aatctacaac tgacttcttt gaaggctttg
      961 cgtattgtgc aggtctatga tgtcttcaat ttggctgagg atttgctggc gcgtgctgag
     1021 aagcatattt tctctgagca ggtgacagac tgtcttttga cggaaactga aatcactgcg
     1081 gagcttgata aggttgcctt ctttgccatt gagtcgcttc ctggtcaatt tgaccaacgt
     1141 gctgttagtt cgcaagaagc tttgctatta tttggaagtg acagtcaggt taaggtcaat
     1201 acagcccagc tttacttggt caataaggat attgcagaag cagagcttga agccgttaag
     1261 aactatcttt tgaaccctgt tgattcgcgt ttcaaggaca ttactttgcc gcttgaagag
     1321 caggctttct ctgtgtctga taagacgatc cctaatcttg atttcttcga gacttataag
     1381 gctgacgatt ttacggctta taaggtagag cagggcttgg ctatggaggt cgatgatctt
     1441 ctcttcatcc aagattattt caaatcaatc gggcgtgtgc caactgagac tgagttgaaa
     1501 gttttggata cgtattggtc agaccactgt cgtcacacaa cctttgaaac tgaattgaag
     1561 aacattgatt tttcagcttc taaattccaa aaacaattgc agacaactta tgacaaatat
     1621 atcgccatgc gtgatgagct tggtcgttct gaaaagccac aaacacttat ggatatggcg
     1681 actatttttg gtcgttatga gcgtgccaac ggtcgtctgg acgatatgga agtctcagat
     1741 gaaatcaatg cctgctcagt tgagattgaa gtagatgttg atggtgtgaa agagccttgg
     1801 ctcctcatgt ttaagaatga gacacacaat cacccaacag aaattgagcc attcggtgga
     1861 gcggcgactt gtatcggtgg tgctattcgt gacccattgt caggacgttc atacgtttat
     1921 caggctatgc gtatttcagg cgcaggcgat atcacgactc cgattgcgga aacacgtgct
     1981 ggtaaattgc cacaacaggt tatttctaag actgcggcgc acggctattc ttcatatggt
     2041 aaccaaattg gacttgcgac aacttatgtg cgtgagtact tccaccctgg cttcgtagcc
     2101 aaacgtatgg agcttggagc tgtggttggt gctgcaccta aggaaaatgt ggttcgtgaa
     2161 aaaccagaag caggtgatgt ggtaatcttg cttggtggta aaacagggcg tgacggtgtc
     2221 ggcggtgcga caggttcatc taaggttcaa acggttgaat ctgtggaaac agcaggagca
     2281 gaggtacaaa aagggaatgc catcgaagag cgtaagattc aacgtctttt ccgtgctggc
     2341 aatgtcactc gtctgatcaa gaaatcaaat gacttcggtg caggtggtgt ctgtgttgcc
     2401 atcggtgaat tggctgacgg tcttgaaatc gatttggaca aggtgcctct taaataccaa
     2461 ggtcttaatg gtactgaaat tgcaatctca gaatctcaag agcgtatgtc agtcgttgtt
     2521 cgtccaagtg atgtggatgc cttcatcgca gcctgcaaca aggaaaatat cgatgcagtc
     2581 gtagttgcga ctgtgactga aaaaccaaat cttgtcatga cttggaatga tgaaacaatt
     2641 gttgatttgg aacgccgttt ccttgatacc aatggtgtcc gtgtcgttgt tgatgctaaa
     2701 gtcgttgaca aggacttgac agttccagaa gcacgcataa catcagcaga gacacttgaa
     2761 gcagatacgc ttaaggtctt gtctgacctc aaccatgcta gtcaaaaagg tctccaaacc
     2821 atctttgact catctgttgg tcgttcaacc gttaaccacc caatcggtgg tcgttaccaa
     2881 atcacaccga cagaaagttc tgttcaaaaa ttgccagttc aacatggtgt gacaacaact
     2941 gcatctgtta tggctcaagg ttacaatgct tatattgcag agtggtcacc ttatcacggt
     3001 gctgcctatg ctgttattga agcgacagct cgcttggtag caacaggtgc tgactggtct
     3061 cgtgcacgtt tctcttacca agagtatttc gagcgtatgg ataaacaggc agagcgtttt
     3121 ggtcagccag tatcagctct tcttggttct attgaggctc agattcaact tggcttgcca
     3181 tcaatcggtg gtaaggactc tatgtctggt actttcgaag acttgacagt accaccaacc
     3241 ttggtagctt tcggtgtgac aacagcggac agccgcaaga ttctctctcc tgagtttaaa
     3301 gcggctggtg agaatatcta ctacattcca ggtcaagcta tttcagaaga tattgatttt
     3361 gaccttatca aggctaactt cagtcagttt gaagctattc aaactcaaca taagattaca
     3421 gctgcctcag ccgttaaata cggtggtgtc ctagaaagtt ttgctctcat gacttttggt
     3481 aaccgtatcg gtgcttctgt tgaaattgca gagcttgaca gcagcttgac agctcaactc
     3541 ggaggttttg tctttacatc agctgaggaa attgctgacg cggtgaaaat tggtcaaact
     3601 caggcagact ttacagtcac tgtcaatgga aatgaccttg ctggcgctag ccttctagca
     3661 gccttcgaag gcaaattgga agaggtttac ccaacagaat ttgaacagac agatgttctt
     3721 gaagaagttc ctgctgtggt atcagatact gttatcaagg ctaaggaaac aattgaaaaa
     3781 ccagtggttt acattccagt cttccctggt accaactcag agtacgattc agctaaggcc
     3841 tttgaacagg ttggagctag tgtcaacttg gtaccatttg taaccttgaa tgaggttgct
     3901 attgctgagt cagttgacac tatggttgct aatattgcta aggcaaatat catcttcttt
     3961 gctggaggtt tctcagcagc ggatgaacca gatgggtctg ctaagtttat cgtcaatatc
     4021 ttgcttaacg agaaggtccg cgcagctatt gacagcttca tcgaaaaagg tggccttatc
     4081 atcggtatct gtaatggttt ccaagccctt gttaaatcag gtcttcttcc atacggaaac
     4141 ttcgaggaag ctggtgagac aagtccaact ctcttctata acgatgctaa tcagcatgtt
     4201 gccaagatgg ttgagactcg tatcgcaaat accaactcac cttggttggc aggagttgag
     4261 gtcggcgata ttcatgccat tccagtttca catggtgaag gtaaatttgt tgtcagcgct
     4321 tctgaatttg cagagctaag agacaatggt caaatctgga gccaatatgt ggactttgac
     4381 ggacaaccat ctatggattc taaatacaat ccaaacggct ctgtcaatgc catcgaaggg
     4441 attaccagca agaatggtca aatcatcggt aagatgggac actcagaacg ctgggaagac
     4501 ggactcttcc aaaatattcc tggtaacaaa gaccaaaccc tctttgcaag tgctgtaaaa
     4561 tactttacag ggaagtaaaa aggttcagtg aaccttttta gccctcgcct tgaattagtg
     4621 aagggcaagg ttagaaagaa tctcgcttaa tttttgagaa atagtaaaca cgggcgataa
     4681 gttgtgcgaa aaactttcta ttttcgcttt acttgacagc ctttgtatcg aggtaaaaat
     4741 gacatacgaa gtaaaatctc taaatgaaga atgtggagtc tttggtatct ggggacatcc
     4801 tcaggcagct caagtcactt actttgggct tcatagcctt caacatcgcg gtcaagaagg
     4861 agctggtatt gtttcaaatg acaatgggaa actctatggt tatcgaaatg ttgggctcct
     4921 ttctgaagtt tttaagaatc aatctgaatt agataattta actgggaatg cggctattgg
     4981 acatgttcgg tatgctactg caggttctgc agatattcgc aatattcagc cttttcttta
     5041 taaatttcat gacgggcaat ttgctttatg ccataatggt aatttgacaa atgctatttc
     5101 cttaaggaaa gaattagaaa agcaaggtgc aattttcaat gcctcctcag atactgaaat
     5161 tttgatgcac ttgattcgtc gaagccataa ctcaagtttc atgggaaagg taaaagaagc
     5221 tctaaataca gtaaagggtg gctttgccta tctactgatg acagaaaata aactcattgc
     5281 tgctcttgac cctaatgcct ttcgtccttt gtcaattgga caaatgcaaa atggtgcctg
     5341 ggttatttcc agtgagacct gtgcttttga ggtggtaggc gcaaaatggg ttagagatgt
     5401 tgaacctggc gaagttattc ttatagacga tcgtggtatt caatgtgatc gttatactga
     5461 tgaaacgcaa ctggcaattt gttcgatgga atatgtctat tttgcaaggc cggattcaac
     5521 tatacatggt gttaatgttc atacggctcg aaaaaatatg ggaaagcgtc ttgcacaaga
     5581 atttaaacag gatgctgata ttgtaattgg tgtcccaaat tcgtccttat cggctgctat
     5641 gggctttgct gaagaatccg gattaccaaa tgagatgggt cttgtaaaaa atcagtatac
     5701 gcagcgaacc tttattcaac cgacacaaga attaagggaa caaggtgttc gaatgaaact
     5761 atcagcggta tctggtgttg tcaaaggaaa gcgcgttgtt atgattgatg actcaattgt
     5821 aagaggaacg acttctagaa ggattgtcgg attattaaga gaagcaggag ctagtgaagt
     5881 acatgttgct atagctagtc cagaattaaa gtatccttgt ttttatggta ttgatattca
     5941 aacaagacga gaattaattt cagcaaatca cgctgctgac gaagtgtgtg atattattgg
     6001 cgcagatagt ctgacctatc tctcactaga tgggttgatt gaatcaattg gactcgaaac
     6061 aaaagcacca aatggtggtt tatgcgtagc ttactttgat ggacattatc caacaccgct
     6121 ttatgattat gaagaagaat acctcagaag tttagaagaa aaaacaagtt tttatattca
     6181 aaaagtgaaa taagctctgc taattatata aggagaaaac atgtctgaaa aaaatgctta
     6241 tgcaaaatct ggtgttgatg ttgaagctgg ttatgaagtg gttgaaagaa taaaaaaaca
     6301 cgttgctcgt acagaacgtg cgggagtcat gggagctcta ggtggctttg gtgggatgtt
     6361 tgacttgagt aaaacaggag ttagagagcc tgttttggtc tcagggacag acggtgttgg
     6421 cacaaaactc atgcttgcta tcaagtacga caagcacgac acaatcggtc aagactgtgt
     6481 tgccatgtgt gtcaatgata ttattgcagc aggtgctgag cccctttact tccttgacta
     6541 cgtagcgaca ggaaaaaata atccagtcaa atttgaagaa gttgtttctg gcgttgcaga
     6601 aggttgtgtt caagcaggag cagcactcat tggaggtgag accgctgaaa tgcctgggat
     6661 gtatggcgaa gatgattatg accttgctgg ctttgctgtt ggtgtggctg aaaaatctca
     6721 gctcatcgac ggttcaaaag taaaggaagg cgatattctt ttgggacttg cttcaagtgg
     6781 tatccattca aacggttatt ctttggtacg tcgtgtcttt gctgactata ctggtaaaga
     6841 gctgcttcca gaacttgaag gaaaacaact taaggatgtc cttcttgagc caactcgtat
     6901 ttatgttaga gcagctctgc cattaatcaa ggaagaacta atcaacggta tcggccacat
     6961 cacaggtggt ggctttatcg agaatgttcc tcgtatgttt gcagatgatt tggctgcgga
     7021 aatcgatgag gacaaggtgc cagtgcttcc gattttcaag gcgcttgaaa aatatggtga
     7081 catcaagcac gaagaaatgt ttgaaatctt caatatgggt gtcggtctta tgctagctgt
     7141 tagtcctgaa aatgttaacc gcgtcaaaga gctcttggat gaaccagttt atgaaatcgg
     7201 tcgtatcatc aagaaagcag acgatagtgt ggtgattaaa taagtcacta aattttataa
     7261 gaaaggagtg tttagttagt ttgcctaact gaacacggga ttaaatttct aggaaaaaag
     7321 ataaaatgac ttagacgcaa gcgtcgttgt tcgttttcct atttttctac gaaatttttc
     7381 ggcaagccga atcatccctt tgtatcgaat atgaaaatcg ctgtttttgc ttctggtaat
     7441 ggttccaact ttcaggtcat agcagagcag tttccagtta gttttgtctt ttcagatcat
     7501 cgtgatgcct atgttttaga gcgtgctcag aatttagcca ttccaagctt cgcttttgaa
     7561 ctcaaggagt ttgagaataa ggcggcttac gagcaagcca tcgttgattt gttggacaaa
     7621 cacgagattg atttggtctg tctaacgggc tatatgaaga ttgttggaga aaccttactc
     7681 ttggcctatg aggggcgtat tatcaatatt cacccagcct acctgcctga atttccaggt
     7741 gcccacggta tcgaggatgc ttgggaagct ggtgttgacc agtctggcgt gaccatccac
     7801 tgggttgact ctggtgtgga taccggtcag gtcatccaac aagtgcgcgt gccacgccta
     7861 gcagacgata gcctagaaag ctttgaaacc cgtatccatg aaaccgaata tcaactctac
     7921 ccagctgtct tagatagctt gggagtggag aggaaagtaa tctagccttt atcttaaaat
     7981 tttaggtaaa gttaggaaag atagtaaaga agaattaacg ttccaaaaag aaaggaattg
     8041 aacccgtctg gaaggcacag aaaaaagata aacatctcct aggcgcaagc gtcttcgtcg
     8101 agtttcctat ttttccttgg ccttctagtc ggacttggta tcttaaatat gactaaacgt
     8161 gctttaatct cagtgtctga caagtcagga attgttgact ttgcaaaaga attgaaaaac
     8221 ttgggttggg atattatctc aactggtggg actaaggttg cccttgataa tgctggcgtt
     8281 gagaccattg ccatcgacga tgtgactgga ttcccagaga tgatggacgg tcgtgttaag
     8341 accctccacc caaacattca cggtgggctt ttggctcgtc gcgacgctga cagccacctt
     8401 caggctgcta aggacaacaa tatcgagctg attgacctcg tggttgtcaa cctctatccc
     8461 ttcaaggaga ctatccttcg tccagatata acctatgatt tggcggtgga aaacatcgac
     8521 atcggcggtc catcaatgct tcgctcagcc gctaaaaatc acgctagcgt gaccgttgtg
     8581 gttgacccag cggattatgc gactgttttg ggagaattgg ctgacgctgg tcagacgaca
     8641 tttgaaacgc gtcaacgctt ggcagctaag gtctttcgtc acacagcagc ctacgacgct
     8701 ttgattgctg agtacttcac aactcaagtg ggagaggcta agcctgaaaa attaaccatc
     8761 acttacgacc ttaaacaggc tatgcgttac ggagaaaatc cacaacaaga cgctgatttc
     8821 tatcaaaaag ccttgccaac agactactca attgcttctg ctaaacagct caacggaaaa
     8881 gaattgtcct tcaataatat ccgtgatgcc gatgcagcaa tccgtattat ccgtgatttc
     8941 aaagatcgtc caactgttgt tgtcctcaaa cacatgaacc catgtggtat cggacaggct
     9001 gatgatattg agacagcttg ggattacgct tatgaagctg acccagtttc aatctttggt
     9061 ggaattgtcg tccttaaccg tgaagttgac gcagcgacag ctaagaagat gcaccctatc
     9121 ttcttggaaa tcatcatcgc accatcatac tcagaagaag cgctagctat tctcacaaat
     9181 aaaaagaaaa acttgcgaat ccttgagttg ccgtttgatg cccaagctgc cagcgaagtg
     9241 gaagctgagt acactggcgt agttggtgga cttttggtgc aaaaccaaga cgttgtggct
     9301 gaaaatccat ctgactggca agtggtgaca gaccgccaac caacagaaca agaggcgact
     9361 gcccttgagt ttgcctggaa ggctatcaag tatgttaagt ctaacgggat tattattact
     9421 aacgatcaca tgacgcttgg actcggtgca ggtcaaacca accgtgtcgg ctcagtcaag
     9481 attgctatcg agcaggctaa agaccacctt gacggtgccg ttctagcatc agatgccttc
     9541 ttcccatttg cggacaacat tgaagaaatc gctgccgcag ggatcaaagc aatcatccaa
     9601 ccaggtggtt cagttcgtga ccaagactct attgacgccg caaacaaaca tggcttgacc
     9661 atgatcttca caggtgtgag acattttagg cattaataaa aaaatgtggt ctaattaaaa
     9721 ccacattttt tattaaaaga aaatataagt taactataat ttgttagctt tatcagcatt
     9781 tacccatgtt ccacgagatc cgatttgaac ataaatacca ttacttggtt gatcaatttt
     9841 taatacttta taactaccag tgacggtaaa atattcaccc acacggagaa tttgatctcc
     9901 taaaactttt ccttggttat ctgtttcatc taatggtgtg ggatcaatcc agtttagtgg
     9961 agttgggtct cctccggcta attctttatt aacgatcaaa ttattaacaa gctgatctac
    10021 acgaaaaacg ccagggaagg taacagtatc accgacctta atggaaggtt ttgtattgtc
    10081 attttgaacc acagactgtg cgggctcttt aatgggaata tagcgtcggt ttccagaaaa
    10141 actgaggtag ctaagccatg tataacctcc agcagtcacg actttatcgt aataaacaga
    10201 ttgacctgct tcatagtaag caagatcagg gctatcgata ctggtttgtc ctttgactgg
    10261 taatctggtt gtaaagtgat aagttccaga ggggtcaaat gaagcttgag aaatgtgttt
    10321 tagttgtctt gggtaggaat gacttgtctg agatgataag tctttaaaat ggatataacc
    10381 acttacctga gattttggaa tttgacgctt atggtatctt tcagggcctt gtccagcgtt
    10441 gtaattatac tcttcgatag tgacagtgtc tccacggata tcagctaccc atgctacatg
    10501 accgtaagca gcatttgact gataagcgtt tttatcaaac caagcgatag cccctatgct
    10561 tggtgtctta ttcacaggat aaccctgatt tttcgcgata tgtccccacg tgcaggcatt
    10621 accgtagcct ttaggtaact gaaaaccatt agcagagctt aaacgaaaag ctgcaaaaga
    10681 agtgcattgg cggatataca tgttccacga atcgattcca ttgccttttt tccacttact
    10741 cggatagtta tcgcctaaaa cagctgcatg aacaagattt tcctgttgcg aaataagtgt
    10801 agatggcata gtaggtacta gaccattaaa acctaaaagg attactcctg agaccaaaaa
    10861 acgatgaaat tttttcatta attacctcct gtaacttatt attcgaaagc aagaaacaaa
    10921 aaatgttatt tttagatgat tattttgaaa attggcttta cttgatatta aataactgtt
    10981 tgattggcta atagagtgtt ccttatttca agaattgtgg tctactatca catttttaca
    11041 atgtttcaat tattgataac taatacatta tgtaaaaaag cgaacgttta tgatataata
    11101 tctaattata atacgaaaga ggtggatgtt ttgaaattac ttgttgttgg ttcaggtggt
    11161 cgtgaacatg cgattgctaa gaagttgtta gcgtctaagg gtgtggatca ggtttttgtg
    11221 gcacctggta atgatggtat gaccttggat ggtctggact tggtgaatat agtagtttcc
    11281 gaacattcca ggttgattgc ctttgctaag gagaatgaga tttcttgggc ctttattggt
    11341 cctgatgatg cgctagcagc tggtatcgtt gatgatttca acagtgctgg actcagagct
    11401 ttcggtccaa caaaggcagc agcggagcta gagtggtcaa aagactttgc caaggaaatc
    11461 atggtcaaat acaatgtccc aacagcagcc tatggcacat tttcagactt tgaaaaagcc
    11521 aaagcctaca tcgaagagca gggcgtacct atcgtggtca aggctgatgg cttggcgcta
    11581 ggtaagggtg tggtcgtggc tgaaaccgtt gagcaggcgg tagaggcggc gcaagagatg
    11641 cttctggaca ataagtttgg cgattcagga gcgcgtgtgg ttatcgagga attcttggat
    11701 ggcgaggagt tttccctctt tgcctttgtt aatggtgata agttctacat catgccgaca
    11761 gctcaggatc acaagcgtgc ctttgacgga gacaaggggc caaacactgg tggtatgggt
    11821 gcctatgcgc cagttcctca tctacctcag agcgtggtag acacagcggt tgagacgatc
    11881 gttaggcctg tccttgaggg catggttgcc gaagggcgtc cttatctagg tgtcctctat
    11941 gtggggctta tcctgacagc tgatggacct aaggttatcg agttcaactc acgtttcggt
    12001 gaccctgaaa ctcagattat cctccctcgc ctgacttccg atttcgctca gaatattgac
    12061 gatatcatga tgggcattga gccttacatc acttggcaga aggacggtgt gactttgggt
    12121 gttgtcgttg cctcagaagg ctatccactc gattatgaga aaggtgtgcc actgcctgaa
    12181 aagaccgacg gcgatatcat cacctactat gccggagcta agtttgtgga aaatagcgag
    12241 ctacttctct caaacggagg acgtgtctat atgcttgtca ccacagaaga cagcgtcaaa
    12301 gcagggcagg acaaaatcta tacccagcta gcccaacaag acacaacagg tctcttctac
    12361 cgaaatgaca tcggaagcaa agctataaga gaataagact aaccattcaa atattagcgt
    12421 cagcaatcaa cgaccgaagg tcgctccaaa aagaaagaag aaaggaactg aacacgggac
    12481 taatttccta ggaaaaaaat aaaactacct ggacacaagc gtcatcgtta gttttctgcg
    12541 tcgtaggcgt ctgatgctaa agcacctagt cgccctgacg tgggttcgtc ttcgaaatca
    12601 aagatttcgg acttaccgca tatttttcta cggaaatttt cgacaagtcg aaacgcccct
    12661 ttgtatctta attatgaaaa ctccaattat ttctattatc atgggctcca aatctgactg
    12721 ggcaaccatg caaaaaacgg ctgaagttct agacaacttt ggcattgctt acgagaagaa
    12781 agtcgtctct gcccaccgta cgccagacct catgttcaag catgctgagg aagcacgtgg
    12841 tcgtggcatc aaaatcatca tcgcaggagc aggtggggca gctcacttgc ctggtatggt
    12901 ggcagctaaa acaaccctgc ctgttatcgg tgtgcctgtc aaatcacgtg ccctttcagg
    12961 tttggattca ctctattcga ttgtgcagat gcctggcggt gtgcctgtgg caaccatggc
    13021 tatcggagaa gcgggagcga ctaacgctgc cctgacagcc ctccgtattc tctccatcga
    13081 ggatcaaaat ctggctgatg cgctagctca tttccatgag gaacaaggaa aaatcgcaga
    13141 ggagtcaagt ggtgaactca tttaagacca ttgggattat cggtggtggt cagctggggc
    13201 agatgatggc cattgcggct atctatatgg gctacaaggt catcacgctg gatcctgcta
    13261 gcgactgccc tgcctcccgc gttagcgagg taattgtggc gccttacgat gatgttgagg
    13321 ctttgggaca attggctgcg cgttgcgatg tattgactta tgagtttgag aatattgatg
    13381 cggacggttt ggatgccgtt gtgtcagctt gtcagctacc gcaggggacc gatctgctcc
    13441 gcatttccca aaaccgtatc tttgaaaaag actttctggc aaataaggct ggtgtgactg
    13501 ttgctcccta taaggtggtg acgtctagcc ttgacctagg gggacttgat tttaccaaga
    13561 cctatgtcct caagacagcg acaggtggtt atgacggtca tgggcaaaag gttatccgct
    13621 cagcagaaga tttgccagag gcgcagcaat tagctaactc agctcagtgt gtcttggaag
    13681 agtttgtcaa ctttgacctt gaaatctcag tcatcgtgtc tggaaatgga gaggatgtga
    13741 cggtctttcc cgttcaggaa aatatccact gcaacaatat cctgtcaaaa actatcgtgc
    13801 cagctcgtat ctcagatcaa ctggctgata aggctaagaa aatggcggtg cagattgcca
    13861 agaaactcca gctgtcagga actctctgtg tggaaatgtt tgcgaccgca gatgatatca
    13921 ttgtcaatga aattgcccca cgtccccaca actcagggca ctactcaatc gaggcttgcg
    13981 acttttcaca gtttgacacc cacattttgg gcgtgctagg agcgccgctc ccaaccatcc
    14041 acctccatgc tccagccgtc atgctcaatg tcctcggaca acacgtccag caggcaattg
    14101 actatgttgc ccaaaaccct agcgcccacc tccacatgta tggtaaacta gaagcgaaac
    14161 ataaccgcaa aatgggacat gtgacggtgt ttagtgattt gcctgatgag gtggaggagt
    14221 tttaggatgt atctaaagta tatatttaaa ataaaggata ggaatttagt atcggattat
    14281 gaatctttag ttcgaaattt gacaatcaat cttcaaaaac tagttattaa agatagaact
    14341 gcattaatag gggataaaca acttccaatt acgatatatt atcgtgggga gaatgaaaag
    14401 tttcctacgg gaatagtaaa agttgaatca gactcagttg aaggattaga gttatttgat
    14461 aaaacgataa aggaaatagt tgggaatgaa agtagatttg gaaagttcac atgtatatac
    14521 gatggaataa gtacatatta ttcaacagaa atttattctg aacttcatga aattgagcgt
    14581 cgaacacaag agtttttaag ggaagcattt tttctgagcg ataaaagtgg ggaattgaaa
    14641 gaatatcagg caactatcaa aaaaatggat agtagtgatt tctcattcag caaattaatt
    14701 aatgaaattt ttaaagcaag cgatataact gatacattta ttgaccatgt tagagcgtgt
    14761 gttgaatttg gaacagttcc aaatggagat ttattaccaa aaagtttttg ggatagttta
    14821 gatgactctt tactagatga taaagagcaa ggtttagaga ttaataattt attaaatgag
    14881 attcgttcaa caagaaatac aatagctcac tgtaaagagt ttaa
//
LOCUS       DATUYR010000036        14712 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.036, whole genome shotgun sequence.
ACCESSION   DATUYR010000036 DATUYR010000000
VERSION     DATUYR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14712
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.036"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..346
                     /gene="amaP"
                     /locus_tag="VCI33_001127"
     CDS             <1..346
                     /gene="amaP"
                     /locus_tag="VCI33_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEP2832056.1"
                     /translation="RIYTEVQLRHKNKKGTLLLKKSAIESYVATAIQTAGLMPNPTVT
                     AKLYKRKFKIIVKGRLASRVAVADQISGVKEGIEKGLTEFFGINYPVNFKVYVKDIAD
                     SDRKHITRNRVE"
     gene            357..545
                     /locus_tag="VCI33_001128"
     CDS             357..545
                     /locus_tag="VCI33_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEP2832057.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            558..1097
                     /locus_tag="VCI33_001129"
     CDS             558..1097
                     /locus_tag="VCI33_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP2832058.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1138..1338
                     /locus_tag="VCI33_001130"
     CDS             1138..1338
                     /locus_tag="VCI33_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP2832059.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            1349..1837
                     /locus_tag="VCI33_001131"
     CDS             1349..1837
                     /locus_tag="VCI33_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP2832060.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTNAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2000..2641
                     /locus_tag="VCI33_001132"
     CDS             2000..2641
                     /locus_tag="VCI33_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2832061.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(2784..3326)
                     /locus_tag="VCI33_001133"
     CDS             complement(2784..3326)
                     /locus_tag="VCI33_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP2832062.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            3444..4145
                     /locus_tag="VCI33_001134"
     CDS             3444..4145
                     /locus_tag="VCI33_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832063.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4160..6796
                     /locus_tag="VCI33_001135"
     CDS             4160..6796
                     /locus_tag="VCI33_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEP2832064.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWSKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRVALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNANHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            6888..7484
                     /locus_tag="VCI33_001136"
     CDS             6888..7484
                     /locus_tag="VCI33_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP2832065.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLVNL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            7495..8451
                     /locus_tag="VCI33_001137"
     CDS             7495..8451
                     /locus_tag="VCI33_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP2832066.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            8548..9888
                     /locus_tag="VCI33_001138"
     CDS             8548..9888
                     /locus_tag="VCI33_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEP2832067.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEM
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANERVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVRRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFAALKDQ"
     gene            10004..10888
                     /gene="truB"
                     /locus_tag="VCI33_001139"
     CDS             10004..10888
                     /gene="truB"
                     /locus_tag="VCI33_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEP2832068.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARTGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALWVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            10917..11849
                     /locus_tag="VCI33_001140"
     CDS             10917..11849
                     /locus_tag="VCI33_001140"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEP2832069.1"
                     /translation="MEIEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFEKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNCTDSDYLTRNFEGQVYTIKEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            11892..12296
                     /locus_tag="VCI33_001141"
     CDS             11892..12296
                     /locus_tag="VCI33_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEP2832070.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            12298..12576
                     /locus_tag="VCI33_001142"
     CDS             12298..12576
                     /locus_tag="VCI33_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEP2832071.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            12566..13354
                     /locus_tag="VCI33_001143"
     CDS             12566..13354
                     /locus_tag="VCI33_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEP2832072.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            13357..14667
                     /locus_tag="VCI33_001144"
     CDS             13357..14667
                     /locus_tag="VCI33_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEP2832073.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
BASE COUNT         4775 a         2435 c         3057 g         4445 t
ORIGIN      
        1 aagaatttat actgaggtcc agttacgcca taaaaataaa aaaggaacac ttttattaaa
       61 aaaatcggct attgaaagtt atgttgcgac cgctattcaa actgcaggtt tgatgccaaa
      121 cccaacagtc acagctaaat tatataagcg gaagtttaag attatcgtta aaggacgttt
      181 agcttctcgt gtagcagtgg cagatcaaat tagtggcgtg aaagaaggga tcgaaaaagg
      241 tttaaccgag ttcttcggta ttaattatcc tgttaatttc aaagtttatg ttaaagatat
      301 tgctgattct gatcgtaaac atattaccag aaatcgtgta gaataggaga tctatcatgg
      361 aattttatga aaaattcaag taccctatta ttggtggctt ggtggggcta attattgcca
      421 tattgctcat ggcttttggt ttattcaaaa cgctactagc aattattttc attattttag
      481 gaatatatgg tggcctgtat gccaaaaaaa caggtattat tgatcaattt cttaatcgca
      541 aataaaggag acttattatg actgaaactt atattaaaaa cacatctaaa gacttaactt
      601 cagcaattcg tggtcaatta acatatgatg ataaagtaat tgaaaaaatt gttggtttag
      661 cacttgaaaa tgttgatggg ttactgggtg taaatggtgg tttttttgct aacctaaaag
      721 ataaattagt aaacaccgaa tctgtacgtg atggcgtgaa cgtggaagtt ggaaaaaaac
      781 aagttgccgt tgatcttgat attgtcgctg aatatcaaaa gcatgtgccg actatttatg
      841 actctattaa atcgatcgtt gaagaagaag tgaagagaat gaccgatctg gatgtgattg
      901 aagttaatgt taaagtagtc gacattaaaa caaaagaaca atttgaagct gaaaaagtaa
      961 gtttacaaga caaagtttca gacatggcac gttcaacatc tgaatttacg agtcatcaag
     1021 ttgagaatgt taaagcttca gtggataacg gtgttgaaaa acttcaagat caaaaagctg
     1081 agccacgtgt aaaataatca tatgtcgtca attaggtaag ctaaaggagg gtttattgtg
     1141 tcagatgaaa aatataatgc gaaattagat caagctggtg gcaagttaaa agaaggattt
     1201 ggtaagattt ctggagataa atctttagaa actgaaggta aagtagataa agttactgga
     1261 aaagttaaag aggttattgc agacgccaaa gatactgtaa aaggattagc taaaggctta
     1321 gataataaag ataaataaag gggacatgat ggaaaaaact caaattaaaa gcactcttac
     1381 ttatgacgat aaggtcatcg aaaaaattgt cggtcatgct ttggaaaacg ttggcggatt
     1441 actagctgta acaggtggat ttttctcaaa tattaaaaat aatttggtga attctgagtc
     1501 tgtaactgat ggtgtttcag ttgaagttgg tagcaaggaa gtagcagttg atttagcaat
     1561 cattgtagaa tacggtaaag atattcctgc tattgcagaa tctattaaag ctattgtttc
     1621 tcaaaacgtt gatagtatga cacatttgaa agttgttgaa gtcaatgtga acgttgttga
     1681 tattcgtact aaggaagaac atgaagctgc tagcgttact gttcaagatc gtgtcactaa
     1741 tgcagcttca agtacttcac aatttgtatc agaacaaacg gaaaaattaa aagatactat
     1801 ctctgatact gtaaatagtg atgaggcagc taaataagca atcgtctagg aagtattatt
     1861 gactagtggt gataagtttg agctggtatt taccagctct ttttgtcacg gttaaaaaga
     1921 cacggactat tgtcatctct ctaaaaatat gatttaatta gatagaaatt tttcaaattg
     1981 ttagaaagga gtaaattaag tgataagtcc taaaaaagag ataacaagtt caaaatatca
     2041 aaaaattgct atttcagtag ctcagcgtat cgcaaatggc gaatatgaag tgggcgaaaa
     2101 gttaaagtca agaactacta tcgcctcgac atttaatgta tcaccagaaa cggctcgtaa
     2161 aggcctgaat attttagctg acttgaaaat tttgacttta aaacatggta gtggagctat
     2221 tgtgctgtca aaagaaagag ctattgagtt tataaatcaa tacgaatcaa cccattccat
     2281 cgctgtttta aaagaaaaaa tacgtgagac tattaatgat cagggaaaag caatggaaaa
     2341 aatggctgtt ttggtcaatg attttttaat gcaaagtcaa tctgttagta agcaataccc
     2401 attagcacct tatgagatta tttgtagtca agattcggaa cattttggca aatctattgg
     2461 tgtgttaaat atctggcacc agacaggagc aactatcgtc gctattgaac atgctggtca
     2521 gtttatcgtt tcaccaggtc cttattctgt tattgaaaaa ggcgatcata tttatttcgt
     2581 tggtgatgag tcagtcattt cacgaatgaa gacctttttt aatttacgaa aaggattata
     2641 agtttgaggg cccacctcaa actttttttg ttgctgttga gcattattaa aagaggcaga
     2701 tcatcatggt ttgtctttcc aaaatatctg tttagacagt tgttttggat tgggagcatg
     2761 atttcagact cttttgatgt tttttaattg tctagagtaa ctgctttaag aataatatcg
     2821 gcaacctctt cagtggtttc ttgatagcca ttagcaatcc aatagctgat aatgctctca
     2881 atactggcaa ggtaaacttc taaagcatag ggataaggaa tatggtattg attggtcaca
     2941 atgtcttgga aaccagtaat agtagtcaaa aattggtaac agaaatcctt gatgagttgc
     3001 ggaaatttga ggtaagaaat agtagcgagt gcggttataa attctctatg ttctataagg
     3061 taggacaagg tttgattgag gacttgtcga gtgtctgtgt agatttcagc ctgatttaac
     3121 aaacggtaca aatcatccag agtgtcattt ttgaaatgat tcatcatgtc aaatttatca
     3181 gtgtagtgga ggtagaaggt tcctctattg ataccagcct ttttagtaag gtcagagaca
     3241 gtaagagttt caaaagattg ttcagttagt aaggtggtta aggcggtctt tacataagct
     3301 ttggtttcag tatgtctttt ggtcattctc aggtgctcct taaatcaaca gttgcgtcag
     3361 atttgttctt gttaaatact tatggccatt atataataag gctataatta aatcaacact
     3421 atgttcaaat aatggagggg cagatggctt ttattgaatt aaaacaggtt tcaaaatctt
     3481 atcaggtagg agagacgact gtttttgcca accacgaagt gtcttttgag attaataaag
     3541 gagaactagt ggttattttg ggagcatccg gagctggtaa atcgacagtc ctcaatattt
     3601 tggggggcat ggacacagta gatgcaggtc aagtaattat tgatggcaaa gacattgctc
     3661 attacacgtc taaggcctta actcagtatc gtcggaatgc tattggtttc gtttttcaat
     3721 tttataattt ggttcctaat ttgacagcta aagaaaatgt tgaattagca gttgaaattg
     3781 tagcagatgc tttagatcct gtgaccattt taaaggaagt aggactcagt catcgtctgg
     3841 atcattttcc tgctcagctc tcaggtggtg aacagcaacg ggtttcgata gcacgtgcct
     3901 tagctaaaaa ccctaaattg cttctttgtg atgaacctac aggtgccctt gactaccaaa
     3961 caggaaagca aattctaacc ctcttacagg atatggcacg aactaagggg accacggtag
     4021 ttattgtaac ccacaatgct gctattgctc cgatagctga tcgtgtcatt tttatgcatg
     4081 atgcacaggt taccaagaca gtcattaata aggaacctgc aagcattgag acaattgatt
     4141 attaagagga aggtgtcgta tgataaagaa aaccttatgg aaggatattt tgagagctat
     4201 caagaatagt aaagggcgat tcatttcctt atttttccta atggccttag gctcctttgc
     4261 tttagttgga cttaaagtca ccggtccaga tatggaacga acagccagcc gttacctcga
     4321 aagacaccag gtgatggacc taacggtact ggcttctcat caattttccc aagccgataa
     4381 acaagagtta gatacgttaa aaggggctca tttagaatat ggtcatttac ttgatgtcag
     4441 tctaactagc aaccagaaat ctcttaggct ctatagcgtg ccaaagaaag tgtctaagcc
     4501 agtcttggtt aaagggagtt ggtcaaagcg agagacagat ttggttttat cctcctcact
     4561 tgctaaaaac tatcagattg gagatgaact agcagtcacc tcacctatgg aaggtttgct
     4621 gacgacaacc cattttcaag tggttggttt tgcgaattct tcagaagttt ggtccaagtc
     4681 caatttagga agttcctcaa ccggagatgg aagtctctac gcttatgctt ttgttaatcc
     4741 aaacgttttt aaaagtgcct ttaacctgct gcgcatccgt tttagtaatc ttcgtctgac
     4801 aaacgctttt tccaaagact atcaaaaaag ggtaacccaa aaccaagctc atttggataa
     4861 tctacttaaa gataatggcc agaagcgtta tgatgacctc caaaatcagt atgaccttgc
     4921 cttaaaaaat ggcagagtag cacttgcaaa ggaaacagtg aaactagctg cgagtgagga
     4981 gaacttaact tttttagaag gctctgcttt acaagaagct aagcatcaga ttgaacaagg
     5041 caaacaagca ttagccaagg aggaaaagca gttagagcag gtgcaggcta caaaagataa
     5101 gctagaaaaa cccagttacc tgacttataa tcgctcgacc ctaccaggag gagaaggata
     5161 tcatacttat gcaacttcaa cgacctccat ttcaaatgtt ggaaatattt ttcctgttgt
     5221 tctttatctc gtagctgctc tagtagcctt taccaccatg acacgttatg ttgatgaaga
     5281 aagaacaagc tctggtctat taaaagccat tggttattct aacaaggata tcagtttaaa
     5341 gtttcttatc tatgggcttt tagccagttt tttagggaca actttaggta ttattggggg
     5401 aacttacctc ttatctgcct tgatttcaga gatcttaaca ggagctttga ctattggaaa
     5461 gactcacctt tatagttatt ggttttataa tggcatagct tacttgctgg ctatgttatc
     5521 tgctgtttta cccgcctact taattgtcaa aaaggaatta ttcctcaatg cagctcagtt
     5581 attgctgccc aaacctccta gtaagggggc aaaaatctgg ttggaacacc ttacttttgt
     5641 ctggaaagcc ctgtccttta ctcacaaggt gaccatacgt aatatttttc gctataaaca
     5701 aagaatgctg atgaccattg taggcgttgc aggctcagta gcccttttat ttgcaggctt
     5761 agggattcag tcgtcattag ccaaagtagt tgagcatcaa tttggtgatt taacgactta
     5821 tgatattttg gctgtcggtt cggccaaagc gacagcgaca gagcaaactg acttagctag
     5881 ctatcttaaa caagaaccta ttacagggta ccaaaaggta tcttatgcca gcttaaccct
     5941 tcctgtaaag ggattacctg ataagcaaag tatttccatt ttatcaagtt cagctacttc
     6001 tcttagcccc tattttaatc tgctggatag tcaggagcaa aagaaggttc ccattccaac
     6061 ctctggtgtt ttgatttctg agaaattagc ctcctattac aaggtaaaac caggtgatca
     6121 gttggtattg actgatcgga aaggacagtc ttataaagtg acgattaaac aggttattga
     6181 catgacagtt ggccattacc tgataatgtc taatacctat tttaagaatc attttaaagg
     6241 attggaggct gctcctgcct atctgattaa ggtaaaagac aaagatagca agcacataaa
     6301 ggagacagcc agtgacttgt taaccttaaa agcgattaga gcagtttcac aaaacgccaa
     6361 tcatattaaa tctgttcagc tagtagtcac ctctcttaat caggtcatga ccctccttgt
     6421 cttcttgtct attttattag caatcgttat cctttataac ttaacgacta ttaatattgc
     6481 tgagcgtatt cgagaattat ccactattaa agttctggga ttttacgatc aggaggtcac
     6541 cttatatatt tatcgagaaa ctatttcgct atccctagta ggcattcttt taggtatcta
     6601 tttaggaaaa ggcctgcata cttatatcat gacaatgatt tcaactgggg atattcaatt
     6661 tggtgtaaag gttgatgctt atgtttatct agtgccaatt ctagtaatcc ttagcttgtt
     6721 agcggtatta ggtatctggg ttaatcgcca tttaaaaaag gttgatatgt tagaagcttt
     6781 gaaatccata gattgattgg tgaaaagaca gatatagaca gcacttgaaa agttaatcat
     6841 attatgatag tatctaaata ctataattta atagaaagat actatgtatg gaaaatagca
     6901 ttgaggccta tctagcagat agcagagaaa aggtaacatt aggtacagtt gttgattgtt
     6961 ttaaaggaaa agcagtctca agtaaggttg tgccaggtga tgtgggattg gttaatttat
     7021 ctgacatggg caccttaggg attcaatatc accaattaag aactttccag atggatcgta
     7081 ggcagctctt gcgttacctt ttggaagacg gagatgtctt aatcgcctct aagggaaccc
     7141 taaaaaaagt ctgtgttttt cataagcaaa atcgagatgt ggtggcatca tcaaatatca
     7201 ctgtcttacg ccctcaaaaa ctgttaaggg gctactatat caagttcttt ttggactcac
     7261 ctattggtca agcgctcttg gacgcagcgg accatggcaa agatgtgatc aatctctcaa
     7321 cgaaagaatt gttggatatt ccaattcccg ttattccttt agtgaaacag gactatttga
     7381 ttaatcacta cttgagagga ttaacagatt atcatcgtaa actcaatcga gcagaacagg
     7441 agtgggaata tattcagaat gaaattcaaa aaggattagg ttaggagtct tgtcatgggt
     7501 tcagttgaac gtacattaaa aatgaccttg gctaccattg ttgccatttt aattgcttac
     7561 caacttcatc tggattatgc aatgtcagcg gggatcattg ccctgttaag tgttttagac
     7621 acccgtaagt ccagtttggt ggtcgctagg aatcgcttgc tatctttttt cctggctttt
     7681 gggattgcga tgatgtgctt tagcttgttt ggttttacga cagtaggttt tatgtgttat
     7741 ttgttaatta ttattccttt gctctatcac tttcagatag aggcaggact ggttcctata
     7801 acagtacttg tgacacattt gattgctaaa aagagtattg cattacctat tttatcgaat
     7861 gagtttatgc tcttttttgt agggactagt gtcgctttac tatttaatgc ttatatgggt
     7921 ccacaagatc agcaaattcg gtactatcac caaaaagttg aatcggatct gaaaggtatc
     7981 ttataccgtt ttgaaagttt tctactggaa ggaaaaggtc aaaatgaagg gctcttgatc
     8041 aaaaaccttg ataaaatatt agatgaagct ctaaaactag tttatcgaga aaggcataat
     8101 caactatttc agcagaccaa ttatcaagtt cattattttg agatgcgacg acaacaaaat
     8161 agactattgg gacaaatggc tatcaacgta aacacgttaa tgagacaaag taaggaaagt
     8221 attcttttgt cacatctttt tcacgaaact gcttgtcagc taagcgaaca aaatccggct
     8281 ttaaccttga ttgatgatat cgaacaattg cttgaaacct ttcgtcatgg tgatcttcct
     8341 caaactcggg aggaatttga gcgacgtgcg gttttatttc agctcttaca agacttggaa
     8401 cgttttatct tattaaaggt agagttttat caggattatc aaaatgacta aatctctatt
     8461 gcacacttct tatctatgac agcttgggac ttaacttcct gagctttttt gctataataa
     8521 aaacaagaat ctcagaatgg agacaagatg aacgaaataa aatgccctca ttgtcatacc
     8581 ctatttacta tcaatgagtc agagtatagt caattattgg aacaagtcag agggcaagcc
     8641 tttgatgaag aattgcaaaa acggttaatc aatgagatgg ccttgctgga agaaaaagcg
     8701 aagcatcaat tgcatgaggt tgtcgcaaaa aaagaaacag ctattaccag cttgacaaac
     8761 caattagagc aaattgaaaa agaacaagcc tacctcagac aagaagaact ggctaaaaaa
     8821 gaccagctta ttgcttcttt agaagcgaaa cttgataaat tagcctcaca aaatgctttg
     8881 gaactggcca atcaattagc tgagaaggac aaagaagtgg ttagtttgac caatcagctg
     8941 gacaaattag cacttgaaaa agatgccacg ttccagtcaa agcttgctac aatcgaaaaa
     9001 gaacgcgatg gaatcaaaaa ccaattggcg cttcaagcta aagaaagtga attgtcgttg
     9061 gcttcggtgc gcagcgacta cgaagcacag ttaaaagcgg ctaacgagcg agttgaattt
     9121 tataaaaatt tcaaagcgca gcaatctacc aaggctatcg gagagagtct agaactctat
     9181 gctgaaacag agtttaacaa agtaagaagc tatgcttttc ccaatgctag ctttgtcaaa
     9241 gataaccaat tgtctagtcg tgggtcaaaa ggggactata tttaccgaga ggttgatgcc
     9301 aatggtgtag aaatcctctc tatcatgttt gaaatgaaga acgaggcaga caccaccaaa
     9361 acaaaacata aaaatagtga ttttttcaag gagttagata aagaccgtcg cgaaaaagat
     9421 tgtgagtatg ctgttttggt aagcatgtta gaagctgata atgattacta caatacaggg
     9481 attgttgatg tcagtcatga gtatcaaaag atgtatgtgg ttcgtcctca actctttatt
     9541 cagttgatag ggattctgcg aaatgcagcc ctgaatagtt tacattataa gcaagagttg
     9601 gccttgatta aggaacaaaa tattgacatc actcacttcg aagaggattt ggatcaattt
     9661 aagaatgcct ttgctaagaa ctatcagtct gccagcaaca actttaaaaa ggctattgac
     9721 gagattgata aatcgattaa acgcatggag gaagtgagac gctttttaac cactagtgaa
     9781 aatcagttac gcttagctaa taataaatta gaggatgttt cagttaaaaa actgacccgc
     9841 caaaacccaa ccatgcgaga gaaatttgca gccctcaagg accagtaagc ctgaaagtga
     9901 gtctttccct gctaaaattg gttaagcgtt tattttggca aaactgatga taaagcggta
     9961 gaatagaatc agtaacaatt actaattaga gagaaaaata gacatgataa atggaatcat
    10021 taatttaaaa aaagaagccg gcatgacctc tcatgacgca gtttttaaat tacggaagtt
    10081 attacaagaa aaaaagattg gacatggggg gaccttagat cctgatgtgg tcggtgttct
    10141 accaattgca gttggtaagg ctactcgtgt gatcgaatac atgacagaag ctggtaaagt
    10201 ttatgaagga caagtgactc tcggttactc aacaacgact gaggatgcta gtggagaggt
    10261 agttgcgaga tcttctttgc cagctgtgct aacagaagaa ctcgttgatc aaacgatgac
    10321 gacttttctt gggaaaatca cacagacccc acctatgtat tcagccgtaa aggttaatgg
    10381 ccgtaaatta tacgagtatg cgcgtactgg tgaaagtgtg gaacgtccta gaagagaagt
    10441 gaccatttct ctgtttgaac gcaccagccc tttgaatttt actgaagacg gtctttgccg
    10501 attctctttt aaagttgctt gtagcaaggg gacttatgtg cggaccttag cagttgattt
    10561 aggtcgagct ctatgggtag agagtcatat gtccttcttg cagcgatcag cttcagcagg
    10621 attaaccctc gagactgcct atactttggg agaaattgct gatatggttt ctaaacaaga
    10681 gatgtctttt ttgttaccga tcgagtatgg cgttgctgat cttcctaaaa tggttattga
    10741 tgatactgaa ttgactgaaa tttcatttgg tcgccgtctt agtctcccta gtcaagagcc
    10801 actgttagct gcttttcatg gcgaaaaagt aattgctatt ttagaaaaaa gagatcaaga
    10861 atacaaaccc aaaaaagttc ttatttaaat agtttgagta agtagaatgg ataattatgg
    10921 aaattgaata tatcaaagat tatcgtgata ttaaccaaga agatgataca gtcttggtat
    10981 tgggatattt tgatggatta caccgtggtc ataaagcctt atttgaaaaa gcaagagagg
    11041 ttgcaaataa agaagggtta aaagtagtcg tttttacgtt tacagagtct ccgaaattgg
    11101 ctttttcgcg tttttctcct gagttattgc ttcatattac ttatccaaag aagcgttatg
    11161 aaaaatttgc cgattacggt gtaaataaat tatatttggt tgattttact tctaaattct
    11221 ctaaggtctc atctgatcat tttataactc attatattaa aaacctgaaa gcaaagcata
    11281 ttgttgtcgg ttttgactat aagtttgggc ataattgtac agatagtgat tatttaacca
    11341 gaaattttga aggacaagtt tataccatta aggaaataaa agaagatcat cgaaaaattt
    11401 ctgctacatg gataaggaag cttattcaag aaggtaatgt agttaaagca aaccacttgt
    11461 taggctatga cttatcgaca agggggagag tcgttcacgg ggatgcgcgt ggccgtacta
    11521 ttggtttccc aacagccaat ctagctccaa ttgacaacac atacttacca gctgatggag
    11581 tctatgttac aaatgttatt gttgctaata aaatctatcg ctctatgacg agtttaggta
    11641 aaaatgtgac atttgggggg aaagagttgc gtctagaagt taacattttt gactttgacg
    11701 aagagatcta tggagaaatt attgagattg tttggttaga taagattcga gacatggaaa
    11761 aattcgaggg aatcgaagat ttgactgata gattggaata tgataaacga acagcattaa
    11821 attggaaaaa agatagcaaa ttatcttaaa tttttgtata ataaggtaag taaatttgat
    11881 agaggaaaaa tatggttacc ttatttttat cacctagctg taccagttgt cgtaaagcaa
    11941 gggcatggtt agtaaaacac gaagttgact ttcaagagca taatattatt acaagtccat
    12001 taagccgtga tgaattaatg tcaatcctct cttttacaga gaatggaacg gaggatatta
    12061 tttcaacacg ttcaaaggtt tttcaaaaac ttgatgttga tgtagaagag ctttcaattt
    12121 cagatttaat tgatttaatt gctaaaaatc caagtcttct tcgtcgccct attatcatgg
    12181 atcaaaaacg catgcaaatt ggttttaatg aagatgaaat tagagcgttt ttatcgagag
    12241 attatcgcaa gcaagaacta cgtcaagcaa cgataaaagc agaaattgag gggtgatatg
    12301 tcaggtaatt attattatcc acttgactta agttggagca ctgaagaaat atcctcagtg
    12361 cttcattttt tgaataaagt ggagttagca tatgaaaaaa aagtagatgc aaaacaactc
    12421 ctagatagtt ataagactta taaaaccatt gtcaaaagta aagctcagga aaagcaaatc
    12481 gaccgtgatt ttcaaaaagt tagtggttat tcgacctatc aagttgttaa aaaagcgaag
    12541 gcaattgaga aaggattctt ttcccttgga aactaaatac gcatttgcaa ggcaaatcat
    12601 taaagaagca gggcttttta tcaagtcaaa aatgtcagag cagctagata ttcaggtgaa
    12661 aacacaattt gatgatctgg ttaccaatgt tgatcaagaa actcaacagt tactgatgga
    12721 tcgtattcat cagacctatc cttgtgatgc cattttagct gaagaaaatg acgttcgtca
    12781 ccccattaat caaggaaatg tttgggtcat cgatcccatt gatggaacgg ttaactttat
    12841 tgtgcaggga tcacagtttg cagtgatgat tgcctattat gaacaaggaa ttggccaatt
    12901 tgggcttatt tatgatgtga tggcagatca attactagct ggtggtggtg attttgaagt
    12961 tactttaaac ggtgataagc ttccagctta ccaagaaaaa cctttagagc gtagcttaat
    13021 tggctgcaat gcaggcatgt ttgccagaaa tgatcgtaac cttgctcatc taattgcaaa
    13081 gactttaggt gtaagggttt atggtggtgc aggtatctgc atggttaagg tcatgaagca
    13141 ggaattactg gcctattttt catttatcca accatgggat tacgcagcag caaaagtact
    13201 aggcgataaa ctgggttatg ttttactaac tattgacgga tatgagcctg attttcaaac
    13261 acgtcaaaaa atcatgtttg ttcctaaatg ccaactcact aggatagcgt cttttctaac
    13321 aaaggactct gccgtcacac ttgaagatca atagaaatga tgtcattacc aaaagaattt
    13381 attaacactt atcaagcaat actaggcaaa gaattagaag atttcttggc aagttttaac
    13441 caagagccgg tcaatgcttt tcggattaac cccttaaaga atcaactaaa aacctttgaa
    13501 caccccattc caaatacctt atggggatat tatggtaaac tatctggcaa gtctccagag
    13561 catgtgtcgg ggttggttta ttcccaagaa ccagcggctc aaatggtggc acaagtagcg
    13621 gctcctcaaa aggggagtcg tgttttagac ttggcagctg caccaggagg aaaatcaact
    13681 catttattag cttatttaga caatacaggt cttttagtca gcaatgaaat ttctaagaag
    13741 cgcagcaagg ttttagttga aaatatcgaa cgttttggcg ctagaaatgt tgtggtgact
    13801 aatgaatccg cagatcgttt agccaaggta ttttctcatt attttgatac tatcgtcttt
    13861 gatggaccat gttctggtga aggaatgttt cgcaaggacc ctgatgctat tcaatattgg
    13921 catcacgggt atcccgcaga atgcgccaag ttgcaaaaat ctattttaga agacgcctta
    13981 accatgttaa aaccaggagg agaattgata tactccacct gtacttgggc tcctgaagaa
    14041 aatgaagatg ttgtgcagtg gctcttagag acttatacct ttttagaatt ggtcgatgtt
    14101 ccaaaactaa atggaatggt tagtggtatc ggacttccag agactgctag aatgtaccct
    14161 catcgttatc aaggggaagg gcaatttgtg gccaagctaa aagataagcg tcaagaagga
    14221 caaagcacta aactaaaagc acctaaatcc aacttgacca aagaccagtt acgtctttgg
    14281 aaaatgtttg aaaaagacca tctaaacctt accctatcag gaacactaca aacctttggt
    14341 gattacctat acctcttgcc agatggtctt cctgctttag atggccttaa aattgctcgg
    14401 aacggtttag aattaggcgt cttcaagaag aaacgctttg aaccctctta cgccttggga
    14461 ttagctttaa agcctgatga ggtccgatca tctattgagc ttagagagga agactttaga
    14521 ccttatgtgt caggtaacgt cattactttg ccccaatctt atccaacagg ctggtaccaa
    14581 ttactcatta atggcaatgg tctaggattt gccaaggtcg ttgacaagac catcaaaaat
    14641 aatttcccta aagggttaag attttagcgt aaaaaataat attaaaaagt taaatattta
    14701 aagaaaaaat gc
//
LOCUS       DATUYR010000037        14170 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.037, whole genome shotgun sequence.
ACCESSION   DATUYR010000037 DATUYR010000000
VERSION     DATUYR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.037"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..1863)
                     /locus_tag="VCI33_001145"
     CDS             complement(<1..1863)
                     /locus_tag="VCI33_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEP2832074.1"
                     /translation="MIKDTFLKTNWLNISHHIILLVFGFYFSFYSLAKELVSSTAQPV
                     NYYAHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLT"
     gene            complement(1860..3605)
                     /locus_tag="VCI33_001146"
     CDS             complement(1860..3605)
                     /locus_tag="VCI33_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEP2832075.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMGPYFENFEEKEAVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSVFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLFVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
     gene            complement(3602..4609)
                     /locus_tag="VCI33_001147"
     CDS             complement(3602..4609)
                     /locus_tag="VCI33_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP2832076.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTVYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEMSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            complement(4634..5839)
                     /locus_tag="VCI33_001148"
     CDS             complement(4634..5839)
                     /locus_tag="VCI33_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832077.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            complement(5839..6642)
                     /locus_tag="VCI33_001149"
     CDS             complement(5839..6642)
                     /locus_tag="VCI33_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832078.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYVVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            complement(6645..7577)
                     /locus_tag="VCI33_001150"
     CDS             complement(6645..7577)
                     /locus_tag="VCI33_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP2832079.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            complement(7567..8721)
                     /locus_tag="VCI33_001151"
     CDS             complement(7567..8721)
                     /locus_tag="VCI33_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEP2832080.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEDNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDRLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            complement(8840..9694)
                     /gene="rfbD"
                     /locus_tag="VCI33_001152"
     CDS             complement(8840..9694)
                     /gene="rfbD"
                     /locus_tag="VCI33_001152"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEP2832081.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFAMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            complement(9832..10170)
                     /locus_tag="VCI33_001153"
     CDS             complement(9832..10170)
                     /locus_tag="VCI33_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEP2832082.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            complement(10406..11515)
                     /gene="rpoD"
                     /locus_tag="VCI33_001154"
     CDS             complement(10406..11515)
                     /gene="rpoD"
                     /locus_tag="VCI33_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEP2832083.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            complement(11524..13338)
                     /gene="dnaG"
                     /locus_tag="VCI33_001155"
     CDS             complement(11524..13338)
                     /gene="dnaG"
                     /locus_tag="VCI33_001155"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEP2832084.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADMEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPIPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     EILYQRLKQQGYITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            13468..13830
                     /gene="mscL"
                     /locus_tag="VCI33_001156"
     CDS             13468..13830
                     /gene="mscL"
                     /locus_tag="VCI33_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEP2832085.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            complement(13958..14134)
                     /locus_tag="VCI33_001157"
     CDS             complement(13958..14134)
                     /locus_tag="VCI33_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEP2832086.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT         4429 a         2734 c         2323 g         4684 t
ORIGIN      
        1 agtcaataaa ccgacatttt caatgttctt aaacttatcc ttagaacccg atagagctaa
       61 gaatttggaa aaacctaaat tactataaac tgtttttcta ttaaaattgt tagcactagc
      121 aggatgcatc gcaatacgat tttttccttg gtaaaactca ctaatcgtat gaggtttggc
      181 cattttagga aagacttctg aataaagaac agatactgaa gaagaaaaat tataaaaagg
      241 taagcttgtt aacgtttgaa actccatgtt agcagttcca cccccgtagg agtctgactg
      301 catgagtccc gcagttgtgc tatttttgat tgccttgata ttaggtaaaa catcgtggct
      361 aacagtaaca tttgatactc tatcaggatc tgacaaactt tcacttaaaa gataaataac
      421 cgtttggtca gcaatattat tttttctgtc tttattaata tcttctgcta atttttggta
      481 cttctgagcg atactcgcta ttgtttcttg gctataattt gtcggttttt ccattatttt
      541 cttggtcacc tgtcttgtcc aaacatatgc taaagatttg tacctagcat ttgttgaaaa
      601 acctaaccag tttatatcat tatcattatt taaagccgaa ataatcggaa gaccagataa
      661 tattttacct tctttttcat aagaaaatgg tattaacata ctacaagata caataaataa
      721 acctaataca gcacttgccc gtttgacagg gctcataaat aatttccctg ctaaaactcg
      781 gccatgtaga taccaagcta ccacaataca aatagcaagt cctacaacca tttctacaat
      841 caccgatttt ttaacaaagc ttgtcactaa acctaattct tgtaaccaaa caaaatcagt
      901 tactaataac ggctcgcttc tcattgattc ttttaaatca ttaacaacag aaataattgt
      961 tcccagaata agcaaaaaga aggtagttgg ccaatatcta ttcgttatca cgtatgctaa
     1021 aagagcaact agtgtaatag ctactatctg aaaaagagta gctccaggga aaacataata
     1081 tcctagcaat gcttcatcac cttttacgcc atactgaaac gtatagttaa aaactaatgc
     1141 taaaaacaaa ctagtagtag ctgctactga aaatgaaggt ttatttgact taaggtgttt
     1201 tagagcattt aaaaccacgt atatgattgt tgcaaatatg acaagtaaaa ctatcaacag
     1261 tccacttatt tttaacagtg caatttggct attacctgct cttactaatt cagaaaatcg
     1321 agaacttatt agttttaaca gatgtatgtc attaattggc acaactgcta aaatcagagc
     1381 tacagaaaaa gtattacctt gatagcctaa tagcccaaat ctatcttttt ctattgcaaa
     1441 atatgctacc aatattttta ttaaagtggc catcactaaa ataatgagta aagaaggtag
     1501 agcttgaaat tgataaaatt gatttttagt taatgaccag atatcaaacc gtttatcatt
     1561 caggtgctgt gttatttgta cccaatagct gacaattaga taagatatca caataaaact
     1621 agttttcaaa aacaactgtc gactaacttg gcgactcaaa taataagata atccaatcag
     1681 tgatataata tatcccacaa aagaaacatt tagtaaatga gcataatagt ttaccggttg
     1741 tgccgtggag cttactagtt ctttcgccaa actgtaaaaa ctgaaataaa aaccgaaaac
     1801 aagaaggata atatggtgac taatatttaa ccaattggtt tttaaaaatg tgtctttaat
     1861 catttaagtt tctccattaa acgcaagaat atatacttca ttgctttagc gggaccaacg
     1921 ataatatatt ttaatgctcc tttgataccg cccatttgat taaagttaac gtaggtatga
     1981 gctccttcat cttcacgtaa atttaacagt ttattgtcaa taaaaggtgt taactcatag
     2041 ggatttttaa caatcctaaa atcataagaa ttcccccacg ctatatagac aaataaccgc
     2101 tcaatcgcat gtaatataga gttttgtggt aaaggttcac ttggaatatc attttgagtt
     2161 aattctaaat caaaaagtga ttttagagca tcgtatttaa accatacaaa tgttccataa
     2221 ctcataacaa atgtatccat agcttgaaaa tcaatttgtt ttttaacatc catttttctc
     2281 cacaaagaca tcatttcttg agcgattaaa tgctcattcc aagcattgac aatcttatta
     2341 aatctaaaaa aacttggaat atctgcgatt ataataccaa tatcatctgt ctcaaaaacg
     2401 ctaagaatac tatctgctgg tttcacgagc atatcaatca attcttttcg ccaagattct
     2461 cctgcccaaa aatcagcttc ttttgatttt ttcgtatgga aatgaccaat ataatcatat
     2521 tgagaaagtt tatcttttag taataacatt ggataaatat ctcttcctct atttcccgta
     2581 acgcgtatat cagcagtttt accttttctt tgaagtattt ctttgatctc ttttcttttg
     2641 atatcactat ctgttgtaat aaaaagatca taatggaagt tccagtcttc gaaagctgtt
     2701 aaaaattcat ctagcaaatc aacgtaaaaa acatgtaaat gaacagctac cttttgacaa
     2761 gtttgttttg ctaaccgttg gcagtttagg tatttttgag ataaaaggta cttagcatct
     2821 ggcaaagaga tttgcgacat atgtgaaaca ataagatcta taggataatt ggttgtttct
     2881 cgtatcagat tcagtaagta aggagcgata tgctggttag catcaatagc ttttactttt
     2941 ataaaaggca catggtgttt tagaattgct gttggattat aatatgaaaa atccggatgt
     3001 ggcatgaatg acgactcagc ttttctagta tcaaagaccg tctgataact aaagccttca
     3061 tttaagagaa tggatgttaa ttgcgtttca taatgctgaa tcacttcttg aacgttttca
     3121 tattctatta tcgattgcca aaattgttga aaaactttgt tttgaatgac ttgattttta
     3181 aacgtcataa aataactttg gacatgttct ttaaaagctt ttgtacctct attattagta
     3241 attccccaaa agtcaactgc ctctttttct tcaaaatttt caaaataagg acccatctcc
     3301 caaataggac caaaacaagt atcgttcata atcgtcaatg agtcaaactc ttccaacttg
     3361 tcaaatccca tagtaatcaa gccatcgtgc catgcagaaa aatcaaaacc tttatttttg
     3421 cgttgcaaaa actcatcaat aagacagtgg tttttgagac gttttaaatc ttcatgactc
     3481 actttactat ttgagataaa aacaatctta gaaaacagtg atctcatttg ttccaattga
     3541 tagtaaacat gtgcacttat tttattgtat ttgttgaagt gaacatataa taaaatacga
     3601 ttcatttttt tctcctaata aaagcaatga gcttagacgt gtatttccat tgacgtgaat
     3661 taataatggc atggtattgt tgtcttaatt cagatagttg atttttttgt tcttctatga
     3721 cattttgcaa ctccacttca cgtgtttctt gctgctttgt tttatctacg atagtttgtt
     3781 gtaattcttg aataaagcaa ctcaactcat gaattcgtat ttcttgctta gcatcttgtt
     3841 gtaataatag ttcagacatt tcttttgtat ctacctgatt atactttttt aagtaattta
     3901 actgagtatc aagtagtttt tgaatacgat gaatcatttt tggtgtatca gtaggtcttg
     3961 aatcagaatc ggttgtcatg cgataaaccg tcgttgagtc tttaatatag gtcagttgag
     4021 tcagttgaaa taactctagt tgaatgtcaa aagtatcgtc tggtgtatct acattgattt
     4081 tttggtttac ttgtctcatt aaatcagcat caactaacca tgtcgatgcc atcgtcatcc
     4141 cttttaaggc taaaactttt tcgtaagtat ctgtcaaagg tgtatgacca gatgaaaaag
     4201 cattagcata taataagtcg ccttgactat ttacaaagtc aaaatcagta ttacaccatc
     4261 gtgaccgttt ggaagcttct aagacatcaa cttgtttttg gagtttaaaa ctatccgtcc
     4321 agtaatcatc accatcacat ctagcaatat atttgccttt tgcataaagg catgctttta
     4381 tccaagtctt tgttattcca atattagttt cgttaaagag taatttaatt tttcctgaag
     4441 actttttttg ataactcttt aaaatctctc gtgagtcatc tgtagaagca tcatcaataa
     4501 caataatttc aacctcaaaa tctgtcactt ggcttaaaaa actgtcaagt gcatcagata
     4561 tccaaggagc tttgttataa ttggtgcaaa taattgaaac tttatacata aattcccttc
     4621 tagctattac tcattaatga aaaacccact tcccattacg ttgatacaag ccacttgctg
     4681 aatctagagc tgacagatca tcttgatcaa tatcgtttct ttgaatcact atctggggtg
     4741 attgttctgc tgttgaaaag agtagcatct gaccgttctt atctcggaca gttacttcta
     4801 attttagttt tatatcatta agttgagata gttgacattg gtaggaaatg cattttctac
     4861 ctataccttg tgttggataa tctaaagaat tgtcgttgta aacccaaatg tttctatcaa
     4921 tatccgttaa agaaaaggca atgtaggtaa aatcatcttt taaaacttca taagaaactt
     4981 caaatgtgat tgtgtcattt ggtgttattc ttggatttga gattaatgac actttcaaat
     5041 ctttggcaat atcagataca gatatttttt cagcattcat tgcatcctca gctgtctctg
     5101 tattatctac actatactga ttagcaacat caaatggttc tccatatgct ttaacaagac
     5161 catcttcaat aagaactgca cggttacaat acttcttaac agctcccata tcatgtgtca
     5221 ctaaaatagt cgtcttacca ctgtcttttc tttccataaa ataatcatta cattttcgct
     5281 gaaaagcttc atctcctaca gcaagcacct catctaaaat aagaacgtca ccttgggctt
     5341 taatagccac tgaaaatgct aaacgtacct gcatgccaga agaataattt tttaattttt
     5401 gattcataaa atgatgcaac tctgcaaaat caacaatatc attgtacata tcatcaactt
     5461 cgtctttggt aaatcccagc atggcgccgt tcatatagac gttttctcgt cctgttaatt
     5521 ctggattgaa tcccactcca agctctataa aagaaaccat cttcccatct actgtaacct
     5581 gtcctttttc tggaacatag atctgggaaa taattttaag gagcgttgat tttcccgagc
     5641 cgttacgccc aacaattcca aaaaaatcgc ctttataaac atcaaagtta atatctttta
     5701 gcacatgttg ttcagtaaat cctttaatcc ctcttaggcg attaaccaga gtcgtgcgaa
     5761 aacttttagt tgcttctgta ggcagtttaa atgttttact aacatgctgt acttttactg
     5821 caatttctct attaatcatt aaataatctc cgcaaatcta tcggcatttt tcttaaagac
     5881 aaagatgcca ataaataata taacaaatgg tactaaatat ggaataacaa cgtaaaacca
     5941 attggttgac atctgccaaa tcgttacgtt ggctctgtca atcaataaat aacgaaaatc
     6001 ttgaatcatt tgtgctactg gatttagcat caacaacttt gccgccaaag ggtggctatc
     6061 taaaacaaaa gtgatcggat aaatgattgg agtggcatac atacctgctt gtaatagtac
     6121 ttcccagact tgagctaagt cacgataata aacaaagaca ttcgacaata acaatgcaat
     6181 tccaagcact aaaacaacta attctataaa aagaaaaaga gagagataag cataccctga
     6241 tatagtcaca ccatttatca acgcaaaaat taaaacaaca accaaattaa taagaaaatt
     6301 aattaaagct cctaacactg ccgaaaaaac aatgatgtgc ttagaaaagt tcaattttcg
     6361 caacaagtct ccccgagata caatagatac cattcccatg ctagttgctt ctgaaaaaaa
     6421 agaccagatg acatttgcca ataaaagcgc tactggaaaa tgaggtacat ttccacccag
     6481 gcgaagaaaa cgaataaata ccaagtacat aatcgtaaac atcataagcg gttttaagat
     6541 agaccataga tagccaattg cgctcccttg atagcgtaat ttaaaatccg tttttaccat
     6601 ttctctcagt aaaatacggt ttttctttgt taaaaaattc attcttactt tctcctgtaa
     6661 ccaaataagg taacaactaa actacgaaaa ataaaggtgt gaaagatgcg attttttcta
     6721 aaaccgtacc ttttcaaggt agctaagcgt ttggtaaaag gcatatccaa taaagaaaca
     6781 taggccgtta ccaattcatg gtcatttggt tttaatggta aatctaatag taattgagcc
     6841 tgtttttggc ttgaagtaat caaccaccaa tatttgttga ctaaatgatg gggggttaac
     6901 caatttttca tgcgtttaga ccaagttcta gctcccaaca cgttagcatc gtgctgacgg
     6961 tataattctg ttggaatatc aaggtaaact aatttcccag tagcagaagc tagtaaagct
     7021 aaataccagt catgcatcaa taaaccgtca caagtggtcc attcctcagc cagagcatga
     7081 gtaatcatca ttgtgccgcc tgttacggta ttttcggtta attcttgaag taggcttgtg
     7141 tttgcatgac cagattgggt cttaatcata ctgtcatggc aaatagctaa atgttgggtt
     7201 accactttga ggtctgtata aaccaataaa ggagctgtca tttcatgttt ttgagcctcc
     7261 aataaggtca cttccagttt attgtctaac catatatcat cttggtcact gaaaaaatag
     7321 acatctgctt tttggtgctt taagagggtg taaaaattct taataactcc cagattttct
     7381 gtctgcccct cattgatcca ctgaattcgt ttgtcttcct tgacaaatgt gcgaataata
     7441 tcctgggtgc catcagttga tccatcatct ctaatcaata aggtccagtc gttaactgtt
     7501 tgcctttgga tactttgaat ttgttctgct aagaagcgtt ctccattgta ggtggatagt
     7561 aaaatattaa tgttcattta aaaataaagc ctcgtactcc cctacaattt tttcccaagt
     7621 gtaatgttct tgaataatag ctttggcttc tttgcccaga cgatcactat caaaacctgc
     7681 atcaacttgg ttaatcagct ctgctaattg ccctttttgc ttggaccaat acagcgcagc
     7741 agacttagcc accgactggt taaaatcaac tcctaaaacc aaattaagat tggtatgggc
     7801 tagtgcttct agaaggcctg gattagtccc tcctacctca tgaccatgga tataggcata
     7861 agcctgttct cgaatgtagg ctaatagttc tttgtcataa agggtaccaa caaatttaat
     7921 gcgtgggtct ttatcacact ctgtctcagc aagtaattgc ttaaagtaag cattgtcttc
     7981 atgattacaa ataatcacta aatcacgctt agttgaggaa gccataaatt ctttaatagc
     8041 agtttcgtaa ttattttcag gaacaaagcg tcctaaaatg agatagtaat ccttttctct
     8101 aatatcaaag gtttgaaaat aagctctgac cttgctatct gctgttgtca aacgtgaagg
     8161 ttgtgtctgc gttccataag caataaaaca tgttttagac caaggataaa cctgtttaag
     8221 gtagcggtca atgccaatat tatctgaaat gaccaaatca gcctgtctag tcatttgctt
     8281 ttcggaaaac ttcaggtagg cctgcactgg ttttgaccat ttagaccgtt tccactctaa
     8341 cccgtctgga ttgataaaaa agcgtccacc tcgattgtga atctgcttca caaatggcgc
     8401 aataaaagcg cctacagtat tgcccaacac ataaaaaata gggttctgaa tctggtgttg
     8461 gtcgctataa gacagggcat aggtaattgc catcatatca taagcaataa ctctagcagg
     8521 accaagtttt gggggattaa ggtaaaagca atctgccccc ttataatcaa aatgaacctt
     8581 atgcttcgta tcgcttaaac aagcaacgtg ataacgaatg tttttagatg attgatgact
     8641 aatcaactct tcgacaaagg tttcaaagcc accgtatttc gctggtaacc ctctgctacc
     8701 aatgataaaa acatcctgca ttgcatacta ccccattcta aaatataatc agtgatatta
     8761 taccacaatt agaaggctaa tgcgacctta aacccatgaa aaagagttgg ccttagaaag
     8821 ccaactcttt ttagaatact tactttttca gtccttgttg gtaaaatgcc ttcaaggctt
     8881 cttgccatgt tggaataaca aaccctgttg cttttgcctt atcaagattc atggttgaat
     8941 ttaaaggacg tttggctttt gctgggaaag ctgatgaatc aactggcacc acttcaacag
     9001 ccttgtcttt taagatttct ttggcaaaat cataccaagt ggtgtcttcc ttagcatcat
     9061 ttgacaagtg atagtaacca aaggcttttt gattctctgt caaataacac atgaattctg
     9121 ccaaggttct tgtccacgtt ggccgaccgt gttggtcatt gacaacagtc aaacgggagt
     9181 gattttccgc taactgctcc atggcaaaga caaagttttt accataattt ccaaataccc
     9241 aagcagtacg gataatataa aaatgctctg cgtatcgttc aacagctagt tctcctaaac
     9301 gttttgtacg gccatattct gtcttagggt ctggacgatc tgtttctacc cattcttgac
     9361 cgactggttt attgccatca aatacgtagt ctgttgaaat gtaaacaagg gtagccccat
     9421 attttccaca agccttggca atattttctg agcctgttac attaatagcc tcattcaagg
     9481 cttttccttc gtcttctgct gcatcaactg ccgtataagc agcacaatga tagaccaatg
     9541 ttggcttgac ctgcgcaaaa acagcttcga ctttgtcttc atttgtgatg tccatttctg
     9601 cgacatcaac ggctacataa tcaacacctc gttcatctaa taagtatcga agttctgttc
     9661 ctaattgacc attgcttcct gtaattaaaa tcatcagttc tcctttttgc cataggtaga
     9721 acacttttct cagtatctta gtgttctcta acattaacca aaggctttca cctgtatttc
     9781 cttttattct acatcaaaaa agggaatatt tcatcccttt actatcaatg attagcgaat
     9841 acccagcgca atcctagcat agcggctcat tttatcaacc gtccaagcag ggtaccagac
     9901 taatttgacc tcagtcttag tgacttctgg cacatcttgc agggcatcat ggatgtaatc
     9961 tgtcaacaag tctgccaaag gacaacccat tgttgtgagt gtcatatcaa tctctgtata
    10021 accgttatca ttgaaccgaa tttcataaat caaacctaga ttaacaacat caatacctag
    10081 ctcgggatca ataacagttt ctaaagcttc aagaattcta ttcttgatag ctatcacctg
    10141 atcttgtgta tattttggtg tatcagacat ggctttcctt tcattttttg aatatgtcgt
    10201 agataagtaa aaaaaccact aaagctttgc ctatttagag gaattgccct ctgggcttgt
    10261 ttaagatatc aagtccaacc ttaaaaatac ccaccacatt cggtccgatt tctaatttgt
    10321 aggtgtaccg ctgataagtg ccacttagga ttttcgctcc tttcttattt caaaggaacg
    10381 acctaaaatc gtccactgga cgattttagt cctctataaa gtctcttaat tgtttactac
    10441 gactaggatg gcgaagtttt ctaagggctt tagcttcaat ctgtcgaatg cgctcacgag
    10501 taacattaaa gactttacct acgtcctcta acgtacgcat tttaccatcg tcaaggccaa
    10561 agcgtaggcg aagaacgttc tcttcacggt cagtaagcgt atctaaaact tcatccaatt
    10621 gctcacgaag aacaacacgc gtcgtataat ctactggatt ttcaataact tcatcttcga
    10681 taaaatcacc taagtgacta tcatcttctt ccccaattgg tgtttctaaa gaaacaggtt
    10741 cttgagcaat ttttagaatt tcacgaacct tgtctggagt catttccata cgttctgcga
    10801 tttgttctgg cgttggatct tgaccaagtt cttgaagaag gttacgttgt tcacggacta
    10861 atttattaat cgtttcaacc atatgaacag ggatacgaat cgtacgtgct tgatctgcaa
    10921 ttgcgcgtgt tatagcttga cgaatccacc atgttgcata agttgagaat ttgaatcctt
    10981 ttgaataatc aaatttatca acagctttca tgagtcccat gttaccttct tgaattaaat
    11041 caagaaattg cattccacgt ccaacataac gttttgcaat tgagacaaca agacgaaggt
    11101 ttgcttccgc taaacgttgt ttagccatta aatctccctt ggcaactgca acagccaatt
    11161 ctttttcctc ttcacttgtt aaaagcggta ccacaccaat ttctttgagg tacatgcgga
    11221 cagggtcgtt tactttagct gaattactac caattaattc ttcatcagtt aattcttctg
    11281 gttttggttc ttcaacaatg tattttgatg atgggttacc ttctttatcc gtaatcgaga
    11341 taccaccatc tgttaaccgc tcaagtaaat catcaatctg atcagcatcc aaagcaaatg
    11401 gaatgacaag tttttcggtc acgtcatcat caatcgctgt tccttctttt ttgtgatggc
    11461 gaataaactc cgcaacttga acgttaaaag ttgttatttc tttttgtttt gtcataattt
    11521 cctctattcc atttttcgtt tctgcgcaat aaaatgttct agtacttcta gagccgcttg
    11581 atgatcgcct ttgttactag attctctaac ttttttccct tgtttgtgaa gatcgcgctc
    11641 tgctaaaagt ttggcacgtt tggataaaat atcatcaatc tcaccaagag ctacttcttt
    11701 gggaaggttt tcttctaaaa cattgtaata agcacggtta acttcctctg acatctctga
    11761 caaatcataa gatgtaatgt atccttgttg cttcagccgt tgataaagta tttctaaggt
    11821 agaggtatca aaataaaaat catcacgatg tcgaaattca tttaataaat agtcatgatg
    11881 taagagccga tgcatgagat gactttctgt cttagcaata gctgtcaaac tttttggtat
    11941 tggtaaggtc acaagattgc tgacggcttg agctatttga ccttgacgct tttgcctatc
    12001 ttgaatcctt aatgcattta ctgattgttc tacttgaaaa tagtcaaagt ttggcaacaa
    12061 atcagcaatc ttgttaatat acgaatgttg agctgtgatg gatggtgatt gagcaatcaa
    12121 tggtgccatt ttctccacat aaacaatttg tgattgcaga ttgtctacat tagtaggttt
    12181 taggtaatca ataaaaaatt caacactact gatccgtgac tgcttaagca aatctgcaaa
    12241 tgcttctggg gaatgccgtt gtacaaattc gtcaggatcc attttattgg ggattctgac
    12301 aatttcgaca acaaaatctt taagcaattc tagtgatttt gcaatagcat gttgtccagc
    12361 atcgtcacca tcataaatca aaacaacttt tttagtgact tgcttaaggt gattgacatg
    12421 ttcttgagtc aatgccgtcc ccattgaagc aacagcattt tcatagccgg aacggtaagc
    12481 ggcaatcacg tccataaacc cttccattag aaacacttca tgggttttgg caataacagg
    12541 ccttgctttg tccagatgat acaattcata agatttgtta aaaagaactg ttcctcttga
    12601 atttttatac ttggcctgtc tcttttccat atcagctgcc gtccagatac gtcctgaaaa
    12661 ggcaataata tgccctcggt catctgataa gggaaacatg atacgatttc gaaaggcgtc
    12721 gtaaatggta ttggattgat cggacaagtt aaacaatcct gaagcaacca attgaccttc
    12781 ctcgtatttt ttagaaagag cttgataaag ataatctgac tcatctgggg ctaaaccaat
    12841 attgaaatgc tcaattaatt ggtcatccaa gcctctctgg taaaggtact tcctagcttc
    12901 ttgaccaatg gtagtggtca tcaaaactgc atggtaaaat ttagcagcat cctcatgaag
    12961 tgtcatcaaa gcgtgattag ggtgcttgtg ttggctagca agtactgcct gacttggcgg
    13021 tatattaagc gacataccag tcttatcggc aataatctga acactttcta agaaggggac
    13081 ttggcggtat tcctcaataa atttaaaaac atcccctgat tttccacagc caaagcagtg
    13141 aaaaaattgt ctgtcttcaa caacattgaa agagggtgtc ttttccttat gaaatgggca
    13201 aagcccgaga taatgccgcc ctgatcggga aagtttgacc acttctccaa tgacatcgac
    13261 aatattaacg ctatttttta cttgggaaat catttctttg tcaattgcca aatcgtcacc
    13321 tccccataaa aatcccattc tagttttacg ttttattata ccaccgaaac tcttttttgt
    13381 aaatttaaaa actgaagtga ttccattgga ttttgtttac taattatgct atgcttaact
    13441 tagagatatt aaaaggggga atatttaatg gttaaagaat taaaagcatt tttgtttaga
    13501 ggaaacatta ttgaacttgc tgtagcagtt atcatcgggg gagcttttgg tgctattgtc
    13561 acttcttttg tgaacgatat tatcacaccg cttattttaa acccagctct gaaggccgca
    13621 aatgttgaaa acatcactca attgtcatgg aatggtgtta aatatggtag ctttttagga
    13681 gccgttatta attttcttat catcggtacc agtcttttct ttgttgtaaa agccgctgaa
    13741 aaagctatgc ctaaaaaaga aaaagaagct gcagctccta ctcaagaaga attattgaca
    13801 gaaatccgtg atttgttggc tcaaaaataa agcgattttt gataaagtag ttaggcatgt
    13861 ctaactgctt tttttgatat caaaaaagct cccttgcttc ttctggataa aaaataaaaa
    13921 cagttctaat tgtgagaact gtttttattt tttaccatta gaatttttta cgtttgcgag
    13981 ctgcttctga tttacgttta cgttttacag aaggtttttc gtagaattca cgtttacgtg
    14041 attcttgaag agtaccagct ttagtaacag aacgtttgaa gcgacgaaga gcatcgtcaa
    14101 gagattcatt cttacgtact actgtttttg acatgtgttt cacctcctca agatattgta
    14161 acattttcac
//
LOCUS       DATUYR010000038        13914 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.038, whole genome shotgun sequence.
ACCESSION   DATUYR010000038 DATUYR010000000
VERSION     DATUYR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13914
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.038"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(287..1732)
                     /locus_tag="VCI33_001158"
     CDS             complement(287..1732)
                     /locus_tag="VCI33_001158"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEP2832087.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(1915..2625)
                     /locus_tag="VCI33_001159"
     CDS             complement(1915..2625)
                     /locus_tag="VCI33_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEP2832088.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(2756..3253)
                     /locus_tag="VCI33_001160"
     CDS             complement(2756..3253)
                     /locus_tag="VCI33_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEP2832089.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVRKDMAKLRASSLIPDDVVINGAVYDVATGKM
                     TQVME"
     gene            complement(3442..4803)
                     /gene="radA"
                     /locus_tag="VCI33_001161"
     CDS             complement(3442..4803)
                     /gene="radA"
                     /locus_tag="VCI33_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEP2832090.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            complement(4884..5330)
                     /locus_tag="VCI33_001162"
     CDS             complement(4884..5330)
                     /locus_tag="VCI33_001162"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEP2832091.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(5440..6207)
                     /locus_tag="VCI33_001163"
     CDS             complement(5440..6207)
                     /locus_tag="VCI33_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEP2832092.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(6325..8109)
                     /locus_tag="VCI33_001164"
     CDS             complement(6325..8109)
                     /locus_tag="VCI33_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832093.1"
                     /translation="MKTARFFWFYFKCYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTVTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(8112..9818)
                     /locus_tag="VCI33_001165"
     CDS             complement(8112..9818)
                     /locus_tag="VCI33_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832094.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIESGQMVGIVGATGSGKSTLAQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(9811..10260)
                     /locus_tag="VCI33_001166"
     CDS             complement(9811..10260)
                     /locus_tag="VCI33_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP2832095.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            10558..11574
                     /locus_tag="VCI33_001167"
     CDS             10558..11574
                     /locus_tag="VCI33_001167"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP2832096.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     HNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            11607..12506
                     /gene="galU"
                     /locus_tag="VCI33_001168"
     CDS             11607..12506
                     /gene="galU"
                     /locus_tag="VCI33_001168"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEP2832097.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(12605..13276)
                     /locus_tag="VCI33_001169"
     CDS             complement(12605..13276)
                     /locus_tag="VCI33_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEP2832098.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLSPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGVLLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
BASE COUNT         4245 a         3028 c         2514 g         4127 t
ORIGIN      
        1 gcatatacca aaaaaatatt gtacactaat tgattcataa aaaccatttc tttaaatcac
       61 actaatcaaa acctcaccgt ttgctgtcat tatcacgtaa gcatctcacc acctctcttt
      121 caaatccaat ctccaaaata gaaataaact atactatttt ttactttacc aaggtagcaa
      181 gctcagataa cgataaaaaa gactgaagaa cttaatcttc aatctttcgt tgtcgtgact
      241 ttttaactcc ttaaagcgtt taaaaataaa ggtgaacgat acagccttat agcatgtttt
      301 tgatgtgctc aatggattta tcacgtccca ataaataaat cgtatttggt aattctgggc
      361 catgcatttc accagatact gcaatacgaa taggcataaa gaggtttttc cctttgattc
      421 ctgtttcttt ttgaacagcc ttaatttgtg ggaaaatatt ttctggtttg aagtcttcat
      481 ctgacatagc ttccaactta gccttgaaag cttgcaagac tgtcgaaact gtttcacctg
      541 ccatcacttc tttttcggct tctgttaatt cagggaaatc agaaaagaaa aggtcagtca
      601 atggaataat ctcatctgca gattttaatt gaggtttgta taattctact agcttctcag
      661 ctttttctgt caaacggcct gcctcttcaa ggaaaggttt gcagagggca taaactgttt
      721 caaaatccgc atgtttaaga tactcattgc tcatccaatc cattttcttc tggtcaaagg
      781 cagctggcga cttgctaagg cgattttcat caaaaagggc aatcagttgc tcacgagaga
      841 agatttcctc ttctccgcca ggattccagc caagaagggc gatgaagtta aagacagctt
      901 caggcatata accttttttg cggtaatctt caatgaattg gagggtattg gtgtcgcgtt
      961 ttgataattt tttacccgtt tcagaattga tgataagagt catatgacca aactctggcg
     1021 cttcccagcc aagagcttca tagaccatca attgttttgg cgtattggca atatggtcat
     1081 cgccacggat aacgtgagaa atttgcatat catgatcatc aacaacaacg gcaaagttat
     1141 aagttgggta gccatctttc ttttgaataa cccagtcacc accgatgtta ccaccttcaa
     1201 actcgatatc acctttaacc atgtcagtcc atttgtaaat accggactca ttaacagcaa
     1261 gacggactgt tggaacgata cctgctgctt cacgctcagc aatgtactta gctttttcgt
     1321 cagctgacat accaataaat tcattgatat agcgaggcgt ttcgccagct gcttcttggc
     1381 gttcacgctc tgctgccaat tcttcctcag tcacgtaaga cttataggct tttccttcag
     1441 ctaacaattg atcaatatat tgttgataaa gtgctagacg ttcagactgg cggtaatttt
     1501 catgcgtttc tgggctttca tcccaatcca ttcctaacca ttttaggttt tcaagttgag
     1561 aacgttcacc atcttcaaca tgacgtttac ggtcagtatc ttcaatacga atgataaaag
     1621 tacctccgtg acgacgggca taaaggtaat taaatagggc tgtgcgggca ttcccgatat
     1681 gtaaaagccc tgttggactt ggtgcataac gcacacgaat tggtttagac atgcttcttc
     1741 tcctgttaca tatatcactc agaaacctgt ggcacgaaaa acaagcaaca aatgatgatt
     1801 tgagcttgcc aagacctttt cagacttctt ctaaaatctt tcttattata gcatatttta
     1861 cttgcttagg cgaaaaaaaa tcagccctaa aaagctgatt ttatgttatt ttgactaatt
     1921 tgaactagtt ggtagataac gtttaagagt cccagcaaat tgctcaaggt taagactttg
     1981 gatataaatc tcaccatgac ctctaaaggt attaacaact ccttcacctg taccaattga
     2041 ctgcataaag ccattttcta aatgaatatc ataatcaagt tctctgctcc atgctacaac
     2101 atgggcatta tcaatagtca ttgtgccacc atcaagtgtg attttcttga tagatccaaa
     2161 cgagttagca agtaaggtac caaggccttc agttgtcatg acaaaaagtc cgccttgacc
     2221 accaaaaagt gcttttccaa tattttgacg ttccattttg tactgtgctg aaccatctag
     2281 ggctagaaag gcaccatcat ttagacgata ttgtttttca cctagttcta aagcaacaat
     2341 ctgtcctggt gtattgggtg ctaatgctaa tttaccatct ccatttgaca tagcttgtgt
     2401 aatgaacatt gattcaccag aaaccatgga acggccgatg gctccaacta attttcctaa
     2461 accagatccc ttaccattta atttggtatt caaggttaca ttctcagtat gataaaccat
     2521 acttccttgt tgtaagtaaa ctgatccccc atgttccaga tcaatctcaa ctagtggaaa
     2581 ttgcatgttt tgatcaatcg taaatctcat tctattgtcg accatcttac tctccttgtt
     2641 tgtatgaata acttgataca attattataa cgcttcccta tttctttgtc taatattttc
     2701 aataattttt aaaaataaaa aagagctgaa ttcagctcta caacttggct actaactatt
     2761 ccattacttg tgtcatcttt ccagtagcca catcgtagac ggcaccgtta ataacgacat
     2821 catcagggat aagagaggag gctcttagct tagccatatc cttgcggaca ctgtcttcca
     2881 catcttggaa gggaaggaaa tcttgccctg aaacatccac acctaagtgc tcatgaatat
     2941 gcttggcaaa gccttcatta gtaaaagtct gcgcgccaca atctgtatgg tggagaacca
     3001 caatctctcg cgttcccatt tgctgctgag aaataaccag cgaccgaatc atgtcttcgg
     3061 tgacacgtcc gccagcattc cttaaaatat gagcatctcc tagagccagc cccagagctt
     3121 gggccacatg tagccgtgaa tccatacaag tcacaatcgc tactttagtc ttgggtttta
     3181 agggcagatg agctgtccca tgcaaggcca cataagcctg attggcagcc ataaaatgtt
     3241 caaaataaga catcaataaa ctccttttga gaaacagcag agctattttt gctgttagtt
     3301 tgtcttatct taacatcttt tatggaaaat agctggatta acctcttaga cggtcggtat
     3361 cgttcgaaaa gttaaaaaaa caaatgattg ggatactttc ttgacaaatt tcctcctcat
     3421 ttgctcttag tttctgatcc tctagctaaa aactgccttt aaaacctgac caaccgtagt
     3481 cactccaaca acctcaatac cttgagggat atcaatgcct tgcaaggcat ttttaggagc
     3541 atagaccttg gtaaagccaa gctttgctgc ttcattaatc cgttgctcaa tacgggtcac
     3601 tcgccgaatc tcacctgtta acccgatctc gcctaagaaa gcttcttgag ggctggttgg
     3661 tttttcctta taactggatg cgatagccac agcgactgcc aaatcaatgg caggttcgtc
     3721 tagtttgaca ccaccagccg acttgaggta ggcatcttga ttttgtaaga gtaaaccaca
     3781 acgcttttct aaaactgcca ttattaggct tacgcgattg aaatcaagtc ccgtagtggt
     3841 tcgtctggca ttgccaaaaa cggttggtgt cactaaagat tgcacctctg ctaagatagg
     3901 acgacttcct tccatcgtta caacgacagc cgatcctgtt gccccatcta agcgttcctc
     3961 taaaaagact tgactaggat ttaagacctc taccaagccg ccagactgca tttcaaagat
     4021 gccaatctcg ttagtggagc caaagcggtt tttcacagcc cgaagaatgc gaaaggtgtg
     4081 atgcctttcc ccttcaaagt agagaacggt atccaccatg tgttctaaca tacgaggccc
     4141 agccaaggtt ccttccttgg tgacatgacc cacaataaag gttgcaatgt tgttcgtctt
     4201 tgcaagctgc atcaattctg cagtgacttc tctcacctga ctgactgatc cttgaacacc
     4261 tgtaatatca gggctcatga tggtctgaat ggagtcaata attaaaaaat ctggtttaat
     4321 attttcaatt tctgtgcgaa tagcttgcat attggtttcg gcatagaggt aaaattcatt
     4381 gtcaatatca cccagacgct cactccgcag tttaatctgc tctgctgatt cttccccaga
     4441 cgcatagaga accgttccct tattagcgag ctgcgtagat acctgcaaga gtaaggtgga
     4501 ttttccaata cctggatcac caccaattaa aatcaagctt ccaggaacca caccccctcc
     4561 aagaactcta ttaaattcag acatgtcagt ctgtgttctg tgataactaa tattatccac
     4621 atcttttagt ttaaccggtc tggacttttc tccagctaga ctaacacggg catttttgac
     4681 ttctttgacc tctacttctt ccacaaaaga agaccaggcc gagcaatttg gacagcgccc
     4741 taaatacttt ggcgactggt agccacactc ctgacagata aaggttgctt ttttctttgc
     4801 catagtaatc tccctcccct agacaagaca aacccactct taaagaatag gtctactgtc
     4861 ataagcattt ttgttaaatc gctttactgc cctgtcgaac caaaaccacc tgttcgttca
     4921 cctctggctt ggtcaccatc agcaattaaa aaaggcataa aaaccccctg aacgatcctc
     4981 tcacccacag ctaatgtcac agggtgatct gtaatgtttt gcatttgtgc aaaaatatgt
     5041 ccttcattag cctcatttcc ataataatct gcatcaatca cacctactga attaatcagg
     5101 ataatccctt tttttcgtgg atttgacgaa cgatcataga ggtaaagcac ctcaccatct
     5161 tgcatgtagg ccttaacccc tgttggtacc aacttaatct cccctggggc aatggttaca
     5221 gcctcagcaa cagacaggtc ataaccagct gcatgagtcg tctcacgttt tggtagtaaa
     5281 tcaggattag taaagcttga aacaagctcg aaaccacgaa tttttgtcat tatgataggt
     5341 atatcctttc aagtcaatca tatcctctta ttatagacta ttcgtaaaag aatagcaaaa
     5401 aggcggtcaa ctttaaacgg accactttta atcatgccat cacatctcat ccccacgata
     5461 actaaagcgg atgtgggtga aatcattatc caaatccttg tctgccataa aagctttggc
     5521 atcttttttc acttgaacca agtcaatacc ttgcattttg gcagcataga cacagccaca
     5581 atagcattgg cggtaaatgt catactcctc acacatttca acggatctgc gataaccgtt
     5641 gtttttcttg aaatcacttg gcaggtattt ggtggtgtaa accttttgca catcgatgcc
     5701 cacatcatta atggtttgcg aatttttatg aggactgatg gttagggcac tggcaaagta
     5761 gtcaaaacct agttccactg ctttttgagc ggtcttgtct agtcggtaat caaaacagac
     5821 acggcaacgg tccccacctt ctggttcttc ttctaaacct cgcacttgtc gaacgtactc
     5881 attggggaca tagtccgctt cgagaaactg cacggtgttg cctgttttag cgttgaactc
     5941 ggagacaaac tgttgtgtca cataggctct gcgatggtat tcgtccttag ggtgaatatt
     6001 agaattggca aagtaaaccg taatgtcagc aaactgcgtc aggtattcta gggtgtaagt
     6061 ggagcatggg gcgcagcaga catgcattaa aatactggga cgcacggatt ccttagccca
     6121 aaccttggcc atctgttgca tgaccctgtc ataatttatc ttttgattgg gattcatgtt
     6181 agcgagaatc tcttgtaaat caatcattgt ggctactttc taattgtttt ttctcttgct
     6241 tgctattata ccatatcagg aaagaaagct tgactgcttt attgttcacc aaaaaactcc
     6301 gctaagacgc ggagctgata ccatctattc aaagacaaat tgattgtgat acaattcggc
     6361 ataaaagcct ttttgatgca atagctcatg atgatttcct tgctcaatga ccttaccatc
     6421 tttcaacaca ataatgtgat cggcatttaa aatggttttg aggcggtgag caatgacaaa
     6481 gctggttcga cctgccacga tagcttccat ggcccgttga attttacttt cggtaactgt
     6541 atcaacattt gaagtggcct catccaaaat caacacttca gggtcagtca gtagtgtcct
     6601 agcaatagaa atcaactgct tttgacctgt tgaaaagaca ttgtcatcat ctgagacata
     6661 ggtattgtac ccttttggta aggacatgat aaagtcatga atatgggtcg cacgcgcagc
     6721 agtttcaacc atgtcttgac taatggtctg atcaccaaaa cgaatattat ccgtaatggt
     6781 tcctgaaaaa agaactgact cttgcaacac aatccctacc ttttgacgaa gactatccaa
     6841 atcgtagtca cgaatatcac ggccatcaaa ggtaatcgaa cctgcatcca catcgtagaa
     6901 acggttaatc aagttcataa tagtggtctt tccagaacct gtcggtccaa ccacggcaat
     6961 cattttgccc ttgggtgcaa cgattgacac atctgataaa actttttgcc caggaagata
     7021 cccaaaatcg acgtggttaa ttgccactgc ttcttttaag ctggtgaacg ctggtgcatt
     7081 ttgtggacga acttcttcgg tttcatcaaa catttcttga atacgatgag caccggtaaa
     7141 ggccagctgc aattctcccc aactagacgc gatttgcatc atgggttggt aatattgctg
     7201 ggaatattgt acaaaagtaa ccactaaacc aagcgctgcc gctgctggca tagatttgtc
     7261 actgaggaca attgttgaac cgacaaaaat cacgatagcc gtgttaataa ggctcattcc
     7321 attcatgact ggaaataatt gtcctgagaa cagacggcgt ttgaaggtgg cttgtcgaac
     7381 cctttcgtta tgttttaaaa aggctgtcac cgtatcttct tggacacctt gtacaataat
     7441 agccttttgt cctgaaatgg tttcatccat aaaagcattt aaagcactga cttcttgctg
     7501 ttggatattg gtgtattttc ttgccaaacg gatgttaatc actaaaaaaa tgagagcaac
     7561 tggggttgat gcgatggtta acaaagctaa acggctatct tgcctaaaca tcatccagac
     7621 caggccgatg taaagggcaa tattagtcac cacttgaacc aaggattggt tcagcgagtt
     7681 ttgaatatta tccaaatcac tcgtgaaacg agaaagaatc tccccatctt tatggcggtc
     7741 aaaaaaggcg acggttaaac gttctaattt accaaacaag cccttgcgca tgcggttggt
     7801 cgaatgtgag acaacacgtg tgagtaagaa actatatatt agattagcta aaacagtaaa
     7861 gaaataagtc atcaaaagct taaacatcac ggcattaaaa gctgataaat cagggctaaa
     7921 atgtgtctga ccactcatct tagcaacgta ataagcctga ccgatttttc ccaactcagt
     7981 caaggactcc cctaagaaga caggagcttt tacttgtaaa taagttgcta agataacggc
     8041 aacagcaatg acagtaaacg agaaacgata gcatttaaaa taaaaccaga aaaaacgtgc
     8101 tgttttcatc actactcctc ccttcctttt tgtgtctcat aaatttcacg ataaatcgca
     8161 ttattagcca ccaactctgc gtgtgttcct tctccaatta actgtccttg gtcaaggact
     8221 aagatcttat ctgccttaac taccgatgaa attttttgag caacaataac tgtagttgta
     8281 ccttctaatt tatgacttaa ggcttcttga acgcgttttt cagattttgc atcaagagct
     8341 gaagtggaat cgtctaatat aagaattttg ggatgattaa tgacaccacg tgcaatagac
     8401 agacgttgct tttgtccgcc agacaagtta ctacctcgct cttcaacttg gctctcataa
     8461 cggctatcca tacgatcaat aaattctttt gcttgcgcga tttgagcggc tttttgcata
     8521 gcatcaatat cagcttttgc tgagccttgg cgaaggttat cagctatcgt tcctgaaaag
     8581 agaatagcct tttgcaacac gatggaaacc gattggcgca gggttgtttg acttagggtt
     8641 ttaatcggct ttcctcctaa caaaatttgg ccatcttgag gatcaaataa gcgagggatg
     8701 agctgtgcaa gcgttgattt cccagatcct gttgcaccaa cgatgccgac catttgccca
     8761 gattcaatag caaaagaaat gtgcttcaag gttggctcat catcattagg atatgtaaaa
     8821 ctaacatcgt caaatacaat gcttcctgag atggtttctt ccttttcatt ttcaaatgtc
     8881 atggcaggtt ctgtagacaa tatttgccta attctagcca tagaaacaaa agcacgacta
     8941 acttgcatcc ccatggaacc aaccacaata attgaaaaca taatttgcat catataagtc
     9001 atgaaagagg caatattccc aatcactgtt ggatctgttt cgaccatggt cgaaacaaca
     9061 tttatagaaa cataaactgc caaataagac accaacatta aagccggctg cattagtgaa
     9121 aaaccataac caatagataa atttaacgcc aaaagatcat tggacgtttc tttaaattta
     9181 gtgtattgtt gttgttcttg tacaaatgat ttaaccacgc gaacgccacg gagattttcc
     9241 ttagcgatgc gattaatctt atccattaac cgttgaaatt taccaaaacg aggacccatt
     9301 tgacgcatca ccaaacccat gatgagggca attaagataa ccattacaac aatgacccac
     9361 cataattgag ggaatgtctg aactgccata ataaacgcgc caataaaaag gatcggcaaa
     9421 cgaaagagaa tttgaaacat catcatgact aaactttgga tttgattgat atcattagtt
     9481 agcctaacca caagattacc agcattaaaa gcttcaatat tggcatatga aaagtcttgg
     9541 atttttcgaa aggtcttctc cctcatatca gcagatacac cttgagctat tttagcagct
     9601 aaaaccgtat taatcgctcc agacattaac cctaaaaggg caataataag taatggtttc
     9661 ccaacctcat aaattttctc ttgattattt tgaatcacag cgcttagcac agatttaaga
     9721 taatgtggtt gcaagagact acttgcaaca accccaataa ccataaaaaa tgagcctaaa
     9781 gcataccatt tataacgtaa gattgcgtct ttaagcatcg ttgtctcctt tataagttag
     9841 catattttgc ttgagctgtt caattacttt tttcgctgtc agcaactctt cttttgtaat
     9901 ttcttttaaa aaatagtgct cgatatcctt acgacactcc cgcaacaaag gcaatttatc
     9961 tctaccaacc tgcgctaaca ctacttgctt ataacgctta tccacttggg aaggcatcac
    10021 attgataaac ccatttttga ccatacgttt cactaaatga ctagcaactg actttgagat
    10081 acgaagttgt ttttcaatat ctttgacaaa tatttcttga ttttggtgtt tagctaaaaa
    10141 aacaagaaca taaccttgag gacctgctag atgttctaca tcatattttt ttgcaatctc
    10201 atcactaatt tgttcgattt gatgtatcaa ttcacgtaaa tcacctatca cttgtgacat
    10261 ctcttccctc cttattttct ttaatagttt ctatgataag tattttagtt ctctagataa
    10321 ctattgtcaa ttaaaatatc tttattttta aaaataagtt tccgaactta aataactaaa
    10381 aagaacaggg caaagccatg acaacagctc acctgatgaa gcttattaaa caacattatt
    10441 aatatagcta gctctcttgg gaacgcctta ttagccaaga ccatatacct tttctaatag
    10501 agctaatcaa ggtgttatgt tataatagag tgactagctc ttgaaggaga atttagaatg
    10561 acaaaacaaa aagtagctat tttaggacct ggttcttggg gaactgccct ttctcaggtt
    10621 cttaatgaca atggccatga cgtccgttta tggggaaata ttcctgatca aattgaagag
    10681 ataaatacca agcataccaa tagacactac ttcaaagaca ttgtgttaga caaaaacatt
    10741 acggcaactc ttgatttagg tcaagcccta tcagatgttg atgcggtgct ttttgttgtg
    10801 ccaactaaag taacacgatt agttgctaga caagttgccg ccattttaga tcataaagta
    10861 gtggtcatgc atgcctcaaa aggtctagaa ccagagaccc acgaacgttt atcaactatc
    10921 ctagaagaag aaattccagc tcattttcgt agtgaagtgg tcgttgtatc aggtcctagt
    10981 catgctgaag aaactatcgt tcgcgatatt actctcatca cggcagcctc aaaagatatt
    11041 gaagctgcaa agtatgttca gtccctcttt agtaaccact acttccgact ttataccaat
    11101 accgacgtca tcggagttga aacagctggt gccctcaaaa acattattgc tgttggagca
    11161 ggagctctcc atggacttgg gtatggagat aatgccaaag cagcggtaat tactcgtggg
    11221 ttagctgaaa tcacccgtct aggcgtcaag cttggtgctg acccgttaac ctacagtggg
    11281 ttatctggtg ttggtgatct aattgtaaca ggtacttctg tccattcacg taactggcgg
    11341 gctggagctg cccttggacg tggtgaaaaa ttagaagata ttgaacacaa catgggtatg
    11401 gttatcgaag gcatcgcaac aactaaggta gcctatgaaa ttgcccaaga cctcggagtt
    11461 tacatgccaa ttacaacagc tatttataag tccatctacg agggcgctga tatcaaagaa
    11521 agcatcctcg gcatgatgtc aaatgaattt cgttctgaaa atgaatggca ttaagccatt
    11581 cgtcactaaa ataagaggag gtgctgatga ccaaagtcag aaaagctatt attcccgcag
    11641 caggtttagg cacacgtttt ttacctgcaa ccaaggccct tgccaaggag atgctaccta
    11701 ttgttgataa accaactatt caatttatcg ttgaagaagc tctaaaatca ggtatcgaag
    11761 aaattcttat cgttactgga aaatccaaac gctccataga agatcatttt gattctaatt
    11821 ttgaattaga atataattta caggctaaag gaaaaatcga acttctaaag ttagtagacg
    11881 agaccacttc tattaacctt cactttattc gacagagtca tccaagagga ttaggagacg
    11941 cagttcttca agcaaagacc tttgttggca atgaaccttt tgttgtcatg ctaggtgatg
    12001 atctgatgga tattacaaat ccaaatgtaa aaccattaac caaacaattg atcgatgact
    12061 atgaagaaac tcatgctgca actattgccg tcatgagggt tcctcacgag gatgtctcta
    12121 actacggtat cattgctcct caagcaaaag cagtaaaagg actctatagt gtggatactt
    12181 ttgttgaaaa accacaacct caagatgcac ctagtgattt ggcaattatt ggacgttact
    12241 tgctcacacc agaaattttt agtattttag aaaaacaaga acctggtgca ggcaatgaag
    12301 ttcaactgac tgatgctatt gataccctca ataagacaca gcgtgtcttt gctcgtgagt
    12361 ttaaagggaa acgctatgat gttggcgata aatttgggtt tatgaaaact tctttggatt
    12421 atgccctgaa acaccctcaa gtcaaagatg acttgaaagc ctacatcatc caactgggca
    12481 aagcactaga gaaaacaaaa ccataaaaat aaggagggaa aatccctcct tatttttatg
    12541 gtttttgagt cgtttcatcc tactagccat ctgagaagta acagtgctaa ccactcactt
    12601 atttttataa aaacccaagc actaacacac caaccaaaat aataccatag ctcactaaag
    12661 ccaacattcg cttggttttc ttcactgtta tcactctcat cttagttgga aatactatac
    12721 tcagtaacac accacccacg acaccgccaa tatggcctgc catactcacg tttggcataa
    12781 aaagattcat cagtaaattg actacaatta acgtctggta tgacttgccc aaatctttca
    12841 acgcttggtt cttacccaaa aaactcaata ctacaatagc agcaaaaagt ccaaataacg
    12901 acgtcgatgc tcctgcagct actgtttcag gactcaacca aaaagtaaag gcattgccca
    12961 taacacctga taaaacataa agtaataaaa aacggcgtga accccagagg tcttcgacaa
    13021 tttgaccaac aaaatatagt gttaacccat tgacaaaaaa atgaccaaag ccgatatgaa
    13081 taaatatcgg agtcaccaat cgccacaact gatctggcat tgcttttact agtaatccaa
    13141 acatacctcc aacttgataa atagcttgag caccagttgc taaatgtcca tataccactt
    13201 gcattgctat aaaaattaag cctgttaacc ctaaaaggaa aatagtaata ggatatcgtt
    13261 ttaataaccg tgtcatctaa gataaacctc ctaaacagca atatcataaa tcgtttgatt
    13321 tggcaatcgt attggcccgc tgataagctt gattgctaac aacatccact aataaagctt
    13381 gatcattttg acgttttaag tctacatcca tagttcgtaa agactagaag agttctttga
    13441 gaatctcttt tttcatcatc ttcttatcat accataaatt aggaaaaagg ttcaagactg
    13501 acaagaaatt agctctctca aaaaagatta aaaataaagg cattctaaag aatcatttaa
    13561 gaaaacttta aggttactat cgtagaatga aaacatccta aaaagcggtt tacctaaacc
    13621 gttgggagaa taggttttcc ccttgttttc ccttttcata aatctcctgg gcacctatcc
    13681 tcgtaggtgc cctcccttgt ttaaacctga ccagttaacc ggctggttag gtttttaaat
    13741 ttaaaaagtc aacagcaaaa atatgatatc acccgcgtgt tcttagctct ctcaattatg
    13801 ggctaaaaac acgcatcaat caatctattg atgaagtttg ctgctttttg atgatcttta
    13861 ttgtgactat actggtaatc ctattgattt tagttgactt ttacggtaga ttcg
//
LOCUS       DATUYR010000039        13430 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.039, whole genome shotgun sequence.
ACCESSION   DATUYR010000039 DATUYR010000000
VERSION     DATUYR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13430
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.039"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            23..1432
                     /locus_tag="VCI33_001170"
     CDS             23..1432
                     /locus_tag="VCI33_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEP2832099.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCESIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            1610..3304
                     /locus_tag="VCI33_001171"
     CDS             1610..3304
                     /locus_tag="VCI33_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP2832100.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            3512..4366
                     /locus_tag="VCI33_001172"
     CDS             3512..4366
                     /locus_tag="VCI33_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEP2832101.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            4519..5859
                     /locus_tag="VCI33_001173"
     CDS             4519..5859
                     /locus_tag="VCI33_001173"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEP2832102.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHSLANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            5837..6052
                     /locus_tag="VCI33_001174"
     CDS             5837..6052
                     /locus_tag="VCI33_001174"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEP2832103.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            6052..6924
                     /locus_tag="VCI33_001175"
     CDS             6052..6924
                     /locus_tag="VCI33_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP2832104.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            6917..7744
                     /locus_tag="VCI33_001176"
     CDS             6917..7744
                     /locus_tag="VCI33_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEP2832105.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVLASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQEVVAKAHKEFEKHEKE"
     gene            7731..8201
                     /locus_tag="VCI33_001177"
     CDS             7731..8201
                     /locus_tag="VCI33_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP2832106.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            8223..9884
                     /gene="recN"
                     /locus_tag="VCI33_001178"
     CDS             8223..9884
                     /gene="recN"
                     /locus_tag="VCI33_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEP2832107.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            10056..10997
                     /locus_tag="VCI33_001179"
     CDS             10056..10997
                     /locus_tag="VCI33_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP2832108.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFISQNETDKMFENLTDDRLLNKL
                     DELDELDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLR
                     SLSDTALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAH
                     YEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAK
                     RKIALPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            11213..12064
                     /locus_tag="VCI33_001180"
     CDS             11213..12064
                     /locus_tag="VCI33_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP2832109.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            12057..12899
                     /locus_tag="VCI33_001181"
     CDS             12057..12899
                     /locus_tag="VCI33_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEP2832110.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLTKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            12877..>13430
                     /locus_tag="VCI33_001182"
     CDS             12877..>13430
                     /locus_tag="VCI33_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEP2832111.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLF"
BASE COUNT         4349 a         2233 c         2866 g         3982 t
ORIGIN      
        1 gcatagaaga aagggatctg ttatggtttc agtgatgtgg tttcgtagag atttaaggat
       61 tgatgatcaa aaagctttag ctcatgctat tgctagtcaa tcgccaacct tatgtgtgtt
      121 tcaatttaac aaggagcaat taaacctgct acatagtcgg aatcaatcta gttttattac
      181 tagcgttttg gcttttagaa agttattaaa atgtgaaagt attgacttat atctaatgta
      241 tggagactta atgacctgtt ttgagcaatt attgacacaa ttaaaagact ggacagatgt
      301 gtattttaac tatgatgaat ctggatatgg tcgtttacgt gatcaaaaag cagcacaatt
      361 tttcaaaaaa aatgggattg ctgttcatac ctaccaagat cactatttac atggtagtca
      421 agagatcatt aaccaatcag gtcagcctta taaagttttt acgccctact ataggatttg
      481 gcaaaattat ccgaaagaga ctcctattaa agtagaacta tcgcaaggca ggtggttaaa
      541 tcttgagaca ccagatgatg ttttaaggac tgttgagagc tttaaagatg aaaaatatca
      601 ggatgttgca acttttgatg aagcgtcaaa acaactaaat cgatttattc aagaccaatt
      661 agcagcttat cacgctaatc gagattttcc agcgcagcta gggacgagtc ggttgtctcc
      721 ctttttaaga ataggagcta ttggcattcg cacggtatat cacgctgtgc gtcaagcacc
      781 aaatagtcta gggcaagcta cttttttaaa agaattagct tggagagact tttacaatat
      841 ggtctatgtg gcttatcctg atcagaaaac tcagcctatc caaaaagcct tttctcaaat
      901 cgaatgggtt aataatcctg actggtttca gttgtggaaa gaaggtaaaa cgggttaccc
      961 tattgttgat gctgccatgc ttcagttaca aaagacagga tggatgcata atcgtttacg
     1021 aatgatagta gcttcttttt taactaaaga tcttttatgt gattggcgtt taggagagca
     1081 gtattttcaa caacaactaa ttgattatga tgctgccagt aatatcggag gatggcagtg
     1141 ggctgcttcc actggaacag atgctgttcc ttattttaga atatttaatc ctgttactca
     1201 aggtaagcgt tttgatccca aaggcgagtt tattaaagcg tatttgcctc aactagaaca
     1261 cgttccagag aagtatctcc acgaaccgtg gaagatgcca aaataccttc aagagagtgt
     1321 atcctgtatt attggcacag actatcctca accaattgtc gatcatgcta aacaacgtga
     1381 acaagctatt gcaaagtatg agtgggcaaa agaaaaagca aaaatagaat gactgttctc
     1441 attataagag tttaaacaaa aagttatcat gaattaagat tatttaattt aaaaaataag
     1501 ttgactttat ttagattatt tgatactatt cagttgtctc ccttagagaa agcgatttct
     1561 tgttttggag ctgattttta ccatttggtc ttagaatagg atgagtaata tgacttacaa
     1621 cgaggtatat caagaatggt tgcacaataa tgatcttagt gatgatatta aagcagattt
     1681 agcagccata aaagacaatg aggctgagat tcaagatcgt ttttacaaaa cacttgaatt
     1741 tggaacagca gggctaagag gaaaacttgg agcaggcacc aatcgcatga atacctatat
     1801 ggttggtaag gcagcacaag ctttagctaa tactattatt gatcatggac ctgaagcggt
     1861 taaaaagggc attgcggtta gttatgatgt tcgctatcaa tctagaacat ttgcagaatt
     1921 aacatgctct attatggcag ctaacggtat taaggcgtac ctttataaag ggattcggcc
     1981 aacaccaatg tgttcgtacg ctattcgtgc tttgggatgt atctctggtg ttatgattac
     2041 agctagtcac aatccccaag cttacaatgg ttataaagct tattggcaag aaggttctca
     2101 aattttggat gacattgcgg atcaaattgc acaacacatg gctgcactaa ctcagtatga
     2161 ggaaatcaaa caaatgcctt ttgaaaaggc tctggacagt ggacttgtta cttatattga
     2221 tgagagtatt gaagaagcat ataaaaaaga agtgcttggt ttaacgatta atgatactga
     2281 cattgataag tcagtccgag tagtctatac ccctttaaat ggtgttggga atctaccagt
     2341 gcgtgaggtt ttaagacgcc gcggttttga aaatgtttat gtggtacctg agcaggaaat
     2401 gcccgatcct gattttacaa cagttggcta tcctaatccc gaagtcccta aaacttttgc
     2461 atattcagaa aaattaggta aggcagttga tgctgatatt ttgattgcaa ctgatccaga
     2521 ttgtgaccgt gtggctttag aagttaaaaa tgcagtcggt gactatgttt ttctcaatgg
     2581 caataaaatc ggagcgcttt tatcttacta tatcttttca caacgatttg acttaggcaa
     2641 tttaccggct aatcctgttt tagtgaaatc cattgtaaca ggagacttgt cacgggctat
     2701 tgctagccat tatggtattg aaaccgttga aacattgact ggttttaaga atatttgtgg
     2761 caaggcaaac gaatatgatg tgacgaaaca aaaaaactac ctgtttggtt atgaagaaag
     2821 tattggtttt tgctatggca cttttgttcg tgacaaagac gctgttagcg cctctatgat
     2881 gattgtagaa atggcagctt actacaagaa aaaagggcag aatttactag atgttttgca
     2941 gactatttat gcgacatttg ggtattataa tgaacgtcaa attgccttgg agcttgaggg
     3001 gatagaagga caaaaacgca ttgcgaggat aatggaagat ttcagacaga caccaatagc
     3061 tagtgttgca gagatggcgt tagacaagac aattgatttt attgatggct atcaagattt
     3121 tccaaagcaa aactgtctca aattttattt ggatgatggt tcttggtatg cacttagacc
     3181 atcaggaaca gagcctaaaa taaaatttta tttatatact attggccaaa cacaagaaaa
     3241 tagtgcaaca aaattagatg ctattgaagc agcgtgtcgc acaaaaataa atcaagttaa
     3301 ttaaacatca aaaatggata acacttttac actatttttc catataaaca actacaaaag
     3361 gaggtgagac ttttaggcac cttctttttc ttgtaggtgc cactaatcaa tttaatcgac
     3421 tcaaaccaat actaaaaaat aagtaagagt ataaagaaca ggcatctatt tttttgttat
     3481 aatagaaaaa tgaaattaaa gggagatgtc aatgacagaa ctaattgatg gtaaagcctt
     3541 agctcaaaag atgcaacaag agttagcagc taaagtcaac aatctaaaac aaaaaaaagg
     3601 aattgtacca ggcttagccg ttattcttgt aggtgatgat cctgctagtc aggtgtatgt
     3661 ccgtaataaa gagcgtgcag ctcttactgt aggttttaaa agtgagaccg ttagattatc
     3721 agaattcatt tgtcaagaag agcttattgc agtaatcgaa cgttacaatg cagataacac
     3781 tattcatggt attttagtgc agctacccct gccaaatcat attaatgata aaaaaattat
     3841 tctcgccatt gatcccaaaa aagatgtgga cggttttcac ccgatgaata caggtcacct
     3901 ttggtcagga cgtcccttaa tggttccttg tactccatca gggattatgg aattgcttcg
     3961 agaatataat gttaaccttg aaggtaaaca tgccgtcatt attggcagat cgaatatcgt
     4021 tggaaaacca atggcacagc tcttactaga caaaaatgca acagtcacgt tgacacattc
     4081 aagaacacgt caattagaag aagtatgtcg ctgtgcagat gtgttgattg tggcaatcgg
     4141 acaaggtcat ttcataacaa aacaatatat aaaagatggt gcgatagtga ttgatgtggg
     4201 aatgaaccgt gatgataatg gcaagctaat tggagatgtg gcctttgatg aggtggcaga
     4261 agttgcagcg aaaatcaccc ctgtaccagg aggtgtcggt cctatgacga ttgctatgtt
     4321 gctagagcaa acttatcaat ctgccctccg tagtactcat aaatagttat aattagacaa
     4381 ttcaacataa gagaatataa gaagtcagca atggcttcct tttttgtttt tctgctcact
     4441 atttatccag aaaaatggta taattgcatt agtatgcaaa gggactatgt caggtccaac
     4501 taagtgaaag gtggcttgat ggcagattat ttaaccgtca ctcatttgac gaaatattta
     4561 aaattaaaat ttgaccgtga cccttatttg gaacgggttt atctgactgg tcaagtgtcc
     4621 aattttcgaa aacgaccaac tcatcaatat ttttccttaa aagatgaaag tgctgtgatt
     4681 caagccacca tgtgggcagg agtctataaa aaactaggat tcgacctaga agaaggcatg
     4741 aaaattaatg taattgggcg agtccaactt tatgaaccta gtggctctta ctctatcgtg
     4801 attgaaaagg cagagccaga tggtataggt gccttggctt tgcagtttga acaattaaag
     4861 aaaaaattaa cggcagaagg ttattttgag caaaaacaca agcagccctt gccacagttt
     4921 gtatcaaaaa ttggggtcat tacaagtcct agtggtgctg tgattcgaga tattatcaca
     4981 accgtttcac ggcgttttcc aggggttgag attttattat tcccaactaa agtacaaggt
     5041 gatggagccg cccaagaagt agtggctaat attcgaagag ccaatcaaag agaggatttg
     5101 gacttgctca ttgtcggccg tggaggtggc tcgatagaag acctttgggc cttcaacgag
     5161 gaaatagtgg ttcaggctat ctttgaatcg caacttccag tgatttcgag tgtgggtcat
     5221 gaaacagaca cgactttagc ggattttgtg gcagatcgca gagcagccac accaacagca
     5281 gcagcagagt tagcaacgcc tattacaaaa acagatctta tgtcttggat agtagagcgg
     5341 caaaatcgtt cctatcaggc ctgcttgagg cggattaagc aacggcaaga gtgggtggat
     5401 aaactctcgc aatctgttat ttttaggcaa ccagaacgat tatatgatgc ctacctgcaa
     5461 aaaattgatc gccttagcat gaccctgatg aatacgatga aagatcgtct gagttccgcc
     5521 aaagaaaaca aggttcagtt ggaccattcc ttggccaata gtcaattaca gacaaaaatc
     5581 gagcgatacc aagaccgtgt tgctaccgct aagcgtttac tgatggctaa tatggctagt
     5641 caatatgata gtcagctggc tcgctttgaa aaagcacagg atgctttgct atcactggat
     5701 gccagccgaa ttatcgcccg tggttatgca atgattgaaa aaaatcaagc actggtagcc
     5761 tctgtaagtc agataacaaa gggtgatcag ttaaccatta agatgcgtga tggacaatta
     5821 gatgtagagg taaaagatgt caaaaacgaa aacatttgaa gaaaatttac aagatttaga
     5881 gactattgtg aacaaacttg aaaatgggga tgtccctttg gaagaagcta tttcagaatt
     5941 tcaaaaagga atgcttctct caaaagaact tcaaaagacc ttacaagcgg cagaaaaaac
     6001 actcgtcaaa gtgatgcaag ctgatggcac agaagtagat atggatgatt aatggacaaa
     6061 ttagctagaa ttgacgaagc cattcgtcgc tactataaga cgactagtaa cggtgtatct
     6121 gaagagttga ttgatgctat cttgtattct gttgacagtg gtggcaagcg cattcgccca
     6181 cttatcttat tagagatgat tgagggattt ggggtatcgt tacagaatgc tcattttgat
     6241 ttggctgcag cccttgaaat gattcataca ggaagtctga ttcacgatga tttgccagcc
     6301 atggataatg atgattaccg acgtggtaga ctgaccaatc acaaacaatt tggagaggct
     6361 actgctattt tggcgggaga tagcttattt ttagacccct ttgggttaat agctcaagct
     6421 gaattaaata gcgaggttaa agtagcctta atccaagaac tttccctagc ttcaggaact
     6481 tttggcatgg ttggtggtca gatgctagat atgaaagggg aaaatcaagc tttgagtctc
     6541 cctcagttgt cactgattca cctcaataaa accggaaaat tattaacttt tccttttaaa
     6601 gcagcagctc ttgtaacaga acaggctatt actgttcgtc aacaactaga gcaggcggga
     6661 atgctcatcg ggcacgcctt tcagattagg gatgatattt tagacgtgac agctagcttt
     6721 gaagatcttg gaaaaacgcc taaaaaggat ttatttgcag aaaaagctac ttacccaagt
     6781 ttattggggt tagaagcctc ctaccaactg ttgacagaga gtttagatca ggctttgacg
     6841 atttttcaga cactcgaaag cgatgtaggc tttaagcctc aaataattac aaaactgata
     6901 gaagggttac gacttaatgc ctaaagaaag agtagatgtg ttagcctata agcaaggatt
     6961 atttgagacc agagagcaag ctaagcgtgg tgttatggca ggcttagtgg tctctgtgat
     7021 taatggccaa cgctatgaca agccaggtga caaaattgac gatgggactg agttaaaact
     7081 taaaggtgaa aaactcaaat acgtcagtcg tggtggatta aaactggaaa aggggctgca
     7141 cgtttttggt gtatcagttg ctaatcaaat tgggattgat attggcgctt caacaggtgg
     7201 ctttaccgat gttatgttac aagatggggc caaactagtc tatgccgtcg acgttgggac
     7261 taaccagttg gtatggaaac tcagacaaga tccacgagta agaagtatgg aacagtataa
     7321 ctttcgttat gctcagccag aggactttaa tgagggacag cctgtattgg ccagtattga
     7381 tgtgtccttt atttctctca gcttgatttt gccagccctg cataacgttt tgtcagatca
     7441 gggacaggtt attgctctca ttaaaccgca atttgaagct gggcgcgagc agattggtaa
     7501 aaaaggcatc gtcaaggaca aacagattca tgaaaaagtg attcaaaagg tcatggattt
     7561 tgcctcaggt tacggattta cagttaaggg acttgatttt tcccctattc agggtggtca
     7621 tggcaatatc gaatttttag ctcatcttgc taagtcacag acacctgaaa cgttagcccc
     7681 gcatttgatt caggaagttg ttgccaaggc acataaggag tttgagaaac atgaaaaaga
     7741 gtgaacgact tgaattaatc aaaaaaatgg ttttgacgca tccaattgag acgcaacatg
     7801 acctcttaag attattggca gagcatgggt tagaattaac ccaggctact atttcaagag
     7861 acatgaatga aattggtata gtaaagattc cttctggcag tggacgttac atctatggtc
     7921 tttctcaaga tagtggaaaa aagatcgttc aaggacctag atcgataaag agcactattt
     7981 tagctgtatc tgacaaaaca aaaggtttag aacaacacct ctatttaaaa gtcgtacctg
     8041 gcaatagtaa attaatcaaa cgttatttat tagcagattt ttcaaaagct atttttagtc
     8101 ttattgctga tgatgatagt ttattgttaa ttgcaaagtc tccttcagaa gcagatatga
     8161 tacgccaaga aattttgctt tggatgcagg gtataaccta gaaaaaaata ggggagaaag
     8221 tgatgttatt agaaatttct attaaaaatt ttgctattat tgatgaaatt tccctaaatt
     8281 ttgaaaatgg tatgacggtt ttgacgggtg aaactggagc aggaaagtct attatcattg
     8341 atgctatgaa tatgatgctt ggtgcgcgtg ctagtacaga agtgattcgt cgtggagcta
     8401 ataaagcaga aattgagggg ttcttttcgg tagatgctac cccagaactg gttgcgtgtc
     8461 tggaatcatc aggcattgct atggaagaag aactgattat tcgccgagat atctttgcca
     8521 atggcagaag cgtgagccgc attaatggtc agatggttaa cctagccacc ttaaaacagg
     8581 tcggacagtt tttggtagat attcatgggc aacatgacca agaagaatta atgcgaccac
     8641 agctccacca gcaaattttg gacgcttttg gtgataaggc ttttgagcaa ttgaaagaga
     8701 actatcagct tatttttgat cgttataaaa gcttgcgtcg ccaggttatt gacaaacaaa
     8761 aaaatgaaaa ggaacacaaa gatcgtattg atatgttggc ctttcagata gcagaaattg
     8821 aagctgctgc cttgagtcga ggtgaggacg accggttaaa tcaagaacgt gatcgcttaa
     8881 tgaaccacaa acaaattgct gataccctga ccaatgccta cgtcatgcta gataatgacg
     8941 atttttcaag tctatccaat attcgctcta gcatgaatga cttactatca attgagcagt
     9001 ttgattcaga gtacaaaggg atgtcgactt cgatttctga agcctattat attcttgaag
     9061 aagtgagcaa gcaattatca gataccattg accaactgga ttttgatggt gggcgattac
     9121 aggaaattga atttcgcttg gatacactca atagcttaac ccgtaaatac ggtggtaatg
     9181 tgaatgatgt gcttgactac tacgataata ttatcaaaga ataccagcta ttaacaggag
     9241 acgacttgtc ttcaggggat ctagaatcag aattgaaagc cttagaaaaa caattggttg
     9301 ctgcagccac taacttgagt gaacgccgcc atcaattggc agagcaatta gaagctgaga
     9361 ttaaagctga acttaaggaa ctttatatgg aaaaagcaga tttcaaagtt cactttacca
     9421 catcaaaatt caatcgtgat ggtaatgaaa gcttggaatt ttacatctct actaatccag
     9481 gcgaaggatt taagcccctt gtcaaggtgg cttctggagg agagttatca cgccttatgt
     9541 tagccatcaa ggctgctatt tctagaaaag aagataagac gagtattgtt tttgatgaag
     9601 tagatacagg tgtttctggt cgcgttgcgc aagccattgc ccaaaaaatt tataaaattg
     9661 ggcgacatgg gcaagttctc gctatttcac acttacctca agttattgct attgctgatt
     9721 atcagtattt tatttctaaa gaaagtaaag aagagtccac cgtttctaag gtaaggttat
     9781 taactcctga agaacgtgtg gaagaaattg ctagcatgat tgcaggaaca gatatgacac
     9841 aagctgctct tacgcaggct cgtgaactac ttgccaaaca ttaagattgt gtttggtatt
     9901 tgatattttc ataattagat gacattgatt ttttaggtgc taaaataatc attgtgctag
     9961 accatttttc ctctgttaat ctggcttggt ttgccttgac aaacctctga gaaatagtag
    10021 aatagataag agaattttaa gctgagggga tagaaatgac tagtaaaaaa gcgtgtttat
    10081 caagcatcat tgtgttagca agtttaacgt gtggaaatga tactgttagt gccaatcatc
    10141 tctcagcaac tggagataag tttgatgatt gctcaacact tgttgaaaaa gatgtggccc
    10201 ctaaagatga acttgagatg ttagcatggt cctcgtctca aacaactgat gatgctgaca
    10261 gagactatga agattttctc gatgatgatt cttttatttc tcaaaatgaa actgataaga
    10321 tgtttgagaa tttaactgat gataggttat taaataaatt agatgaatta gatgaattag
    10381 atgaattaga tgaagaagat acaattgagc cagagcaaaa tgtaataatg cctagtgacg
    10441 atgagctatt tgatttaact gatgctgttg agacacgcct tactgtttct agtgctcccc
    10501 atttagaggc tgaattgccg aaaccacatt tgaggagcct atcagataca gcactgcggt
    10561 ctggtgaaat tagaggacat ttagataaca aactggacgc tttgtctgta acagctacaa
    10621 agttagcatt aacgatggct caaaaatttg atttgacaac gcatgtctat tctataggtg
    10681 aaagctttag tgaagtatta gctgctcatt atgaagacag aaaagcagaa tcagcttttt
    10741 ctaagaaaaa gagatttcac cttcctattg ctactccaga tgttgttata gaggagttaa
    10801 ggcgcctagt ctcttctatt ggaagttcaa aagaagatgt ttcagttcct tataatcgga
    10861 agctaggtat ggcagttgca aaaagaaaaa tagccctgcc acaaacggga gaggagttct
    10921 cttattatcc agctttactt ggtttaatga tattaggatt aacgccgatt atgataccaa
    10981 agaagataaa taattaggta ataagttatt gtagttgtaa aagacggggc aaatgttcaa
    11041 gcgtgaaaag gttgactttt gcctcgtttt gtatctcgtt attgctaagg cattttcttc
    11101 gagcagctag tgctcgccat acctatctac cgagtgatta ttcaatagca ctaccagttt
    11161 tgctttattt agtgaggagt gttagaataa agaggtgaga aactagttta gaatggaaaa
    11221 atacatggga actataaaaa ttgttacaga ttcatcaata actattgaac cagaattaat
    11281 taaagcttta gatattactg tagtgccttt atctgtcatg attgatagca aattatattc
    11341 tgataatgat ttaaaagaag aaggtcattt tttgagtctt atgaaggcta gcaaatccct
    11401 tcccaaaacc agtcagccac cggttggtct ctttgctgaa acttacgaaa atttggtcaa
    11461 aaaaggagta actgacattg ttgctattca cctctcacca gctttatcag gtactattga
    11521 agcttcccgt cagggggctg aaatcgctga ggcccctgtt acagttttag attcaggatt
    11581 tactgatcaa gccatgaaat ttcaggtcgt tgaggcagca aagatggcaa aagcgggtgc
    11641 tagtttgaat gagattttgg ctgctgttca agctatcaaa tcaaaaacag agctctacat
    11701 tggtgtatct accttagaaa atttagtaaa aggaggtcgt attggtcgtg tgacgggtgt
    11761 tttgagctcg ctacttaatg tcaaagttgt tatggcactt aaaaatgatg agttaaaaac
    11821 gcttgtcaaa ggtcgaggta ataaaacatt tacgaaatgg ttagatagtt acctcgctaa
    11881 aaatagtcat cgcccgattg cagagatagc tatttcttat gctggtgaag ctagcctggc
    11941 attaacctta aaagaaagaa tcgcagctta ttacaaccac tctatttcgg ttttagaaac
    12001 aggctctatc atccaaacac atacaggaga aggagctttt gcggttatgg ttcgttatga
    12061 ataatcgtca tttatttagt gggatatttt tctttgttat tagtttatgt ctggcctttt
    12121 tattgctaaa tattattatc cctaagtcaa attcacgttt gaaaaagagt gattttctga
    12181 caaaagaaca agtagctatc caatatgttg ctataggaga ttcattgaca gaaggagtag
    12241 gtgatctaac tcatcaaggt ggttttgttc ctttgttaac gaatgatctc agtgaatatt
    12301 ttaaggctaa tgttaatcat caaaattacg gcgtatctgg tgataccagt caacaaattc
    12361 ttgataggat gataaaacaa aagcagatac agttatcttt aaaaaaagca gatataatga
    12421 cgttaaccgt tggtggtaat gatgttatgg cagttattcg gaagaattta gcggatttgc
    12481 aagtttctag ttttagaaag ccagctcgtc agtatcaaaa acgattaaga cagattatcg
    12541 agttagccag aaaagataat aaagatcttc ctatttttat tttaggcatc tataatccgt
    12601 tttatttgaa ttttccagaa ctaactgata tgcaaaaagt gattgatgac tggaatacca
    12661 aaactaagga ggttgttgga gaatacgatc gtgtgtactt tgtgccaata aatgacctct
    12721 tgtataaagg gataaatgga caagaaggaa ttgttcattc ttcaggagat caaactacaa
    12781 ttgtcaatga tgccttgttt actggggacc attttcaccc aaataatact ggctatcaaa
    12841 tcatgtcaaa tgcagtaatg gagaaaataa aaaagcatga aaaaaaaatc aaaccttaat
    12901 tggtggaagt ggagcttctt atgtctgctt gcttttaata ccgccttttt gatggttatt
    12961 gcaagtcgtc tcattcaagt aagagaacct gagtcagaac tgattgctaa aaaaccagtt
    13021 aaaaatatca aaattggtac atttgtcaca accagagaac agcttaacga aactgtagca
    13081 agttatctta aagattatca gactaaaaaa atgtcttata aattttatgc gacatcttct
    13141 tctattttgt ttgaaggaac ttatcaatta ttagggtatg aagtcccttt atatatctat
    13201 tttcaacctc atcgtctaga aaatggagct gtccaactac aggtgatctc tttttcagtg
    13261 ggaactctgc cactcccaga aaaagatgtt ttgcagtatt tgaaatcaag ctacaaattg
    13321 ccaagctttg ttaaagtgat gccaaatcaa tcagctatcg ttgttaattt acaagatatc
    13381 caaaatgatg ctaaggttta tttaaaggcc aagaaaattg atttatttaa
//
LOCUS       DATUYR010000040        13370 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.040, whole genome shotgun sequence.
ACCESSION   DATUYR010000040 DATUYR010000000
VERSION     DATUYR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13370
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.040"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            21..683
                     /locus_tag="VCI33_001183"
     CDS             21..683
                     /locus_tag="VCI33_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEP2832112.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGSVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            664..2906
                     /locus_tag="VCI33_001184"
                     /pseudo
     CDS             664..2906
                     /locus_tag="VCI33_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
     gene            2977..4017
                     /locus_tag="VCI33_001185"
     CDS             2977..4017
                     /locus_tag="VCI33_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEP2832113.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILDRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            4114..4719
                     /locus_tag="VCI33_001186"
     CDS             4114..4719
                     /locus_tag="VCI33_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEP2832114.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            4886..5112
                     /locus_tag="VCI33_001187"
                     /pseudo
     CDS             4886..5112
                     /locus_tag="VCI33_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5097..5345
                     /locus_tag="VCI33_001188"
     CDS             5097..5345
                     /locus_tag="VCI33_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832115.1"
                     /translation="MDADLAQMDALGLYYDDNHLSLVNTVKVYLKEFGIGESQIAFSY
                     KDLNSGRTAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            5342..5932
                     /locus_tag="VCI33_001189"
     CDS             5342..5932
                     /locus_tag="VCI33_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEP2832116.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVYSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQGLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLITLYSSGL
                     GGATQASEEVNGLGYVQLVQLTYVINEWHRVNHNMI"
     gene            complement(6046..7266)
                     /locus_tag="VCI33_001190"
     CDS             complement(6046..7266)
                     /locus_tag="VCI33_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEP2832117.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQNLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(7273..8301)
                     /gene="queA"
                     /locus_tag="VCI33_001191"
     CDS             complement(7273..8301)
                     /gene="queA"
                     /locus_tag="VCI33_001191"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEP2832118.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            8503..9207
                     /gene="nagB"
                     /locus_tag="VCI33_001192"
     CDS             8503..9207
                     /gene="nagB"
                     /locus_tag="VCI33_001192"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEP2832119.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIKANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            9326..10042
                     /locus_tag="VCI33_001193"
     CDS             9326..10042
                     /locus_tag="VCI33_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP2832120.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPIHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            10337..11299
                     /locus_tag="VCI33_001194"
     CDS             10337..11299
                     /locus_tag="VCI33_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEP2832121.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFCCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            11312..13117
                     /gene="pepF"
                     /locus_tag="VCI33_001195"
     CDS             11312..13117
                     /gene="pepF"
                     /locus_tag="VCI33_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP2832122.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFS
                     GHLLDSSANLLTITDTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
BASE COUNT         4100 a         2384 c         2763 g         4123 t
ORIGIN      
        1 taaaaagatg aggtaaaaaa atgttagacg aattattaat taaaggaaaa tcgtttttga
       61 gagagcggta ttttctgcca tacctaatta gtcttttatt aggacttttt ctaatactaa
      121 gcttcagttt tttgttctgg ggaaataggc agagcaaggc agccgttcct gctttgagag
      181 aaatatcgcc tgtgaaacag caggttagtg aagaaaaaaa ggaaattcaa gaagatagca
      241 gtattttagt ggatttaaaa ggctccgtac aaaaagaggg agtttataag ctaactgcta
      301 gcagtcgtgt gcgagatgtc attgaactag ctggaggcct aacttcagaa gctgataaac
      361 atgctattaa ttttgcagaa aaactaactg atgagcaagt tgtttatgta cctaaacaag
      421 gagaagagat ttctgtgcta ccaagatcat tagtttctgg taaaaaagaa actgccagca
      481 aggatcagtc aaaggttcat attaataagg ccagcttaga agaactacaa catatcccag
      541 gtattggtgc taaaagagct caggatatta ttgatatgag agataagtta ggtggtttta
      601 aagcgttaga agatctccgt caggtttcgg ggattggcga aaaaacgctc gagaaattaa
      661 aagatgacat tttcttggac taagctagtg cccctgtcaa agattcaatt tgctttttta
      721 atcttggttc tcttttatca aatccatagc cctagctggt taacgtttct ccttagcctt
      781 agtttgattt gcttacttgt caagcgtttg tctaaaaaag aatttctcgg tgtctttgcc
      841 atccttagtt tctgtgcttt gtttttgctg tatcagaaac agcagttgat tcaaaaactg
      901 gaaattcagc cagtacaaat aacctctgta gcattagttc ctgatagtat tcggattaat
      961 ggtgatcaat tactgttctt ggacgtcacg ggaaacattc ttatcagtta ttttaccgtt
     1021 tgaaaagcca agcagaagcc caattgttca agaaagagca ccgttggtta gtgatgcacg
     1081 caaaagtgac attggaaaaa gctgaggaag ttcgaaattt taaaggcttt aattatcaga
     1141 cttttttggc ttatcaagga atttacagga tagggaaggt agagcaaata gaacagcttg
     1201 aagtgatttc acccgagtca atatgtgact atttgtcctc tttaagacga cgagccattg
     1261 ttcattgcca gcagcatttt ccaagaccta tgtctcacta cctgacgggc ctcctttttg
     1321 gttatttaga taagtcgttt ggtgagatga cagattatta tagtcagttg ggtatcattc
     1381 atctctttgc tttatcaggg atgcaggttg gcttctttct tacttgtttt agaagggtgt
     1441 tgctattact cgcagttcct ttggaatgga taaaatggat agagcttcct tttgcttgct
     1501 tttatgccgc gttgacaggt tatagtattt ctgttattcg aagcctcgtg cagtcgcagt
     1561 tgcgtcattt aggaataaaa ggattagaca atctggcttg tactttttta cttgtctttt
     1621 tatgggatgc tcatttctta atgactgttg gtggcgtatt aacgtttagc tatgcttttt
     1681 tgttaacagt ggttactgtt gaagaattat caggagctaa aagacagttg gtacaagtct
     1741 tgaccatttc cctaggaata ttaccatttt tgctctttta tttttctagt tttaacccca
     1801 tgtcaatggt cttaacaggg ctcttgtctt atctctttga cctttttatt ttgccccttc
     1861 tttgtctggt tttttgtcta tcccctttgg tgactgtctc aatatgtaac catcttttta
     1921 ttttattaga aaaagtcatt caatttctcg gaaatacttt caatagttcc cttgtttttg
     1981 gaagcccgac cagttggcac ttgcttatct tggtgattag cttcgctatt ttttatgatt
     2041 atcggcaggt gcgacaacga gttatcactt gcggtttggt tatagcgttg acgcttttgt
     2101 ctgtcaaata tcctttgacc aatgaagtga cctttatcga cattggccag ggagatagta
     2161 ttttggtgag ggagtggaca gggaaaaatc tcttaataga tgtaggtggg cgccctttct
     2221 tttcttcaaa agagcactgg cgacgaggac atcacgtagc taatgctcag aaaaccttaa
     2281 ttccttactt aaaaagtaga ggtattcaca caattgacca actattagta actcacgcag
     2341 atactgatca tatgggggat atcgaagtgg ttgccaaagc tattcgaata aaggagattt
     2401 tgactagtca aggcagttta agccatccaa gctttgttag gcggctaagg cgtttaaaat
     2461 gtcacgttag ggttttagca gctggagacc agttgcctat tatgggaagt gtcttacagg
     2521 tactctaccc ttggcagtta ggggacggga aaaataacga ttctttggta ctttatggca
     2581 ggctcttaaa tcgaactttt cttttcacag gtgacttgga aaaagagggg gaaaacgaga
     2641 tcataaaacg ttatcctcaa ctaagagtag attatttaaa agcaggtcac catggatcaa
     2701 atacctcttc tagtgcagct tttttagacc atattcagcc taaagtggcc tttatttctg
     2761 ctggaaaaaa taaccgttat cagcatcctc acagagaaac cttagcccgt ttggaagaca
     2821 gacaaattac ttattaccga acggatacgc aaggagctat tcgtttgact ggttggacaa
     2881 gctggcatct tgaaacggtc cgttagttaa aattccagcc tgacgaagaa acaagcagtc
     2941 gctttccttt taggagaaag atgatataat aaaagcatga ttgcgataga aaagattgaa
     3001 aaactgagta aagaaaattt gggtcttata acccttgtca caggagatga cattggtcag
     3061 tatagccagt tgaaatcccg cttaatggag cagattgctt ttgataagga tgatttggcc
     3121 tattcttact ttgatatgtc tgaggccgct tatcaggatg cagaaatgga tctagtgagc
     3181 ctacccttct ttgctgagca gaaggtggtc atttttgacc atttgttaga tatcacgacc
     3241 aataaaaaaa gtttcttaaa agaaaaagac ctaaaggcct ttgaagccta tttagaaaat
     3301 cccttagaga ctactcgact aattatcttt gctccaggta aattggatag taagagacgg
     3361 cttgttaagc ttttgaaacg tgatgccctt gttttagaag ccaaccctct gaaagaagca
     3421 gagctaagaa cttattttca aaaatacagt catcaactgg gtttgggatt tgagagtggc
     3481 gcttttgaac aactgctttt aaaatcaaac gatgatttta gtcaaatcat gaaaaacatg
     3541 gcttttttaa aagcctataa aaaaacggga aatattagcc taactgatat tgagcaagcc
     3601 attcctaaaa gtttacaaga caatattttt gacttaacca gacttgtcct aggaggtaaa
     3661 gttgatgtgg ctagagattt gattcatgat ttacgcttat caggggaaga tgatattaaa
     3721 ttaattgcta ttatgttggg gcaatttcgc ttatatttac agcttgccat tcttgctaaa
     3781 gaaggaaaat cagaacagca gcttgtaacc agtttgtcag agatacttga tcggcgggtt
     3841 aatccttacc aggtcaagta tgcgttaaag gattctagga ccttatctct tgcctttcta
     3901 acaggagcgg tgaaaacctt gattgagaca gattaccaga taaaaacagg actttatgag
     3961 aagagttatc tagttgatat tgctctctta aaaatcatga ctcactctca aaaataggcg
     4021 ttggaccgct ttccaagaga taatcaaatt agttgaaagc tcttattttt tgcgataaga
     4081 tagacgtatc ataaaggaaa agaggacagg aaaatggcta ttattttacc agaacttcca
     4141 tacgcgtatg atgctcttga accacagttt gatgcggaga caatgactct tcatcatgat
     4201 aaacaccatg ccacttatgt cgctaatacc aatgcggctt tagaaaaaca cccagagatc
     4261 ggagaaaatc tggaggaact attggcagat gtcactaaga ttccagaaga tattcgtcaa
     4321 gccttaatta ataatggtgg cggacaccta aaccatgcgc ttttctggga actattatca
     4381 cctgaaaaac aagacgtcac tcctgatgtt gcacaagcca ttgatgatgc ttttgggtca
     4441 tttgacgcct ttaaagaaca atttacagca gctgctactg gacgttttgg ttcaggttgg
     4501 gcatggttag ttgttaataa agaaggacaa ctcgaaatca catcaaccgc taaccaagat
     4561 actccaattt cagaaggcaa aaaaccaatt ctagcgcttg atgtgtggga gcatgcttac
     4621 tacttgaatt atcgtaatgt acgtccaaat tatatcaaag ctttctttga aatcattaat
     4681 tggaaaaaag tatcagaact ttaccaagcc gctaaataag aaaagggttt ccaatctcaa
     4741 aaagagattg gttttttaag tggttttgtg aataacagtg tggggacatc attttttaga
     4801 aaagataaaa atagtttgtc tttttaatgt aattccttga tttttagcta aatttagcta
     4861 gaatagaatg gataggagat tttaggtgtt aagaaagaag aaaactacca ttgtagggat
     4921 tattttagga atgatgatag tgaccttact tgtcggctgt ttgactggta aaaagaaagg
     4981 ctttcctcct aaagcaggtg agcggcagca aacggctgac ttaaaaatga aaaaagatta
     5041 gaaaaactgt tcaaaaaccg ttacctgaaa aatctaaaga ggctaagcaa gctgttatgg
     5101 atgcagattt agcgcaaatg gatgccttag ggctctatta cgatgacaac catttgagtt
     5161 tggtcaatac ggtcaaggtg tatttaaagg aatttggcat tggtgaatct caaattgcct
     5221 tttcctacaa agacctcaat tcaggcagga ctgctgccat gaatgaccat cagccaatga
     5281 ttgcaggaag cacctataag ctgccattaa acatgctggt ggtagataag gcaaattatc
     5341 catgacagag cgttttgaca ttacagaaac tgattatgaa tatgatcagg aacatcatgc
     5401 ctatgttgct caattcaatg gtgccatgtc tattccggat atgcaagaat attccttggt
     5461 gtactcagaa aacacgccag cttatgctct agcagaacga ttgggtggca tgaataaggc
     5521 ttaccaatta tttgaccgct acgggaaggt atccggagct attacgacca ttgatcgtaa
     5581 tggcaataaa atcaccaccg cttactattt gcaaggtttg gattacttgt ggcagcatca
     5641 ggataaatat aaggatatct tgtactacat tggtgaatct ttccctgatt tgtattacaa
     5701 gacttatttg cctcatgtaa aggtttatca aaaaccgggt tatgtcagag aagccttaaa
     5761 tgtaggtgcc attgtttgcg aagagtctcc ttatttgatt actctttatt catctggttt
     5821 aggtggagca acacaagcat cagaggaagt caatggcctt ggttatgtgc agcttgttca
     5881 gctaacatat gtaattaatg agtggcatag agtcaatcac aacatgatct aatactcagt
     5941 tgacattagt ctgatagtag acatacttac tgatgaaagt cactaaaata agtttttgct
     6001 cattaaaaaa gcttggcctg tttcttcagg tcaagctttt tgagattaat gctgtgtttt
     6061 tgaggtctta ttgtcacctc ttgtttcttg ataatggtga agcaattttt tgaatttttt
     6121 aacggcagcg cgtgttagct gcttagatga tttgccattt ctgctgtaaa tgggattgtg
     6181 cttatccctt tgctgttcta aatcgggaac tagaaaagtt gcatcaggac tattaattgc
     6241 tttttcaata aaagccggat caaaaagcag aatagggtca atatcaagca gacggtgcaa
     6301 gtcatcgttt tggttgccat aattgaaaga agaaccattg acaatactat ttcgcgttag
     6361 gttatcagga tctagtgcta ctaaaaattg tccagattct gacaaaataa actctgagtg
     6421 aaaatgaatt aaaatcttat agttggcttg aggaatattg tctgtataga ctgcctgatg
     6481 attgccatta gcatctaaaa tactagcaac cttattatgg tatctagaag attcataaag
     6541 agcataatcg tcatcttcta gagtagccaa atatttggct aaggcatccg catcggtttt
     6601 ccctttattc ccataatgtg agcgaaccca ctccgtttgc tgggttgata tgaggtaacg
     6661 gagttgatga attttccgat taaaacggct ataaggaatg tgttcgagat taacataata
     6721 agcctgaatc agggtagata attggtccca aaaaggatct gttggcgata gctttggact
     6781 taattgtgcc aatacttgct gtgtttgttt tgattgtcct gtcaactcgt ctggcatctt
     6841 taaatacgtc aagagcagtt gtaaccgctt ttgagcttct tgaggcgtct gtgccatctc
     6901 ttcttgttgc caaagctttt gaaagtcagg tgatcctatc aggcgaatgc gactataaag
     6961 ttgttcgaaa tgataagagt ttcctctaac agcgagttgt tcaaggtatc ctttttggat
     7021 ggctttatag gaccatccgt ccttatcgag ctttcgaaca taatcaagca agtcgtggcg
     7081 taatttaata gcttctgaaa gctggagttc tcgtaattta ggatagtttt ggtaaaaagg
     7141 tgccactaaa ttttgataaa gtttgtgttc tttttcaaag gcaatccgct gatagtcttt
     7201 ctgccaaaaa ctgacgcaaa aagtcagaaa catcaagaag aatagccatt ttccaagtaa
     7261 tttcataggt ttttagttga caaacatggc atcgccaaag ctaaagaaac gatacttttc
     7321 gtcaaccgcg tgtctgtaag cttcaaggac aaagtcacgc cctgcaaagg ctgatactag
     7381 catcacaagg gttgattttg gaagatggaa attagttgaa aaagcatcaa ctaccttgaa
     7441 ttggtagcct ggtttaataa aaatattggt ccaacctgaa tcagcttgaa tgtccccttg
     7501 gaacttacct ccgatggttt ccaacgtacg aatagaggtg gttcctacag cgacaacacg
     7561 accgccagct tgtttgacgt ctcttagggt ttgcgctgct tcttctgaga gggaataaaa
     7621 ttccgaatgc atatcatgct catcgaggtt atccacggaa acaggtctaa aagtcccaag
     7681 ccctacatgt agggtcaaat aaaccaaatg tacccctttg gcttcgattt ttttgagcaa
     7741 gtctgttgtg aagtggagac cagcagttgg ggcggccgca gagccatttt ctttagcata
     7801 aacggtttgg tatcgctcag catcctctaa tttttcgtga atataaggag gcaatggcat
     7861 ttcgccgagg ctctctaaaa cttctaagaa aatcccatca tagctgaatt caacaattcg
     7921 tccaccatgt tctaattcgt cgataatggt ggctttgaga cgcccatctc caaaattgac
     7981 ttggctacca accttgagcc gtttggctgg cttggctaaa acttcccatt gatctccttg
     8041 tgtattttta agcaaaagca attccacatg accatgagta tcgggttttt ctccataaag
     8101 tctggcaggc aagacacggg tgttattcat gacgagggca tcccctggat ttaattgatc
     8161 aatgatatgg tcaaaatggc tatcaaccat agttttttga cggtgatcaa taatcaatag
     8221 ttttgaactg tctctttttt cgagtggggt ttgggcaatc aattcttctg gtaactcaaa
     8281 atcaaaatcg ttagtattca ttgggactcc tttaaagcca ctagtaaatc tcttcctagt
     8341 aactctttca tgtaagataa atcttttcta ttatatcact tgtcgtctaa cccgtcatct
     8401 ttaatctttc atcagaaagt aaaggatagg cttgacaaaa attggtatat accatataat
     8461 aaaaacacga aaggtctata ccaattagaa aaggagaatg acatgaaaat tattcgggtc
     8521 caagatcaga ttgaaggtgg gaaaatcgct tttaccttat tgaaagatag cttagctaag
     8581 ggagctaaga cgttaggatt agcaacagga agttcaccaa ttagttttta ccaagaaatg
     8641 gtcaaaagtc ctttggattt ttcggactta acaagtatta acttggatga atatgtcggt
     8701 ctttcagtag agagcgatca gagttatgat tattttatgc gtcaaaacct ttttaatgcg
     8761 aagcctttta aaaagaatta cttgcccaat ggtttagcaa ctgatatcga agctgaagct
     8821 aaacgatatg accaaatcat tgcagagcat ccgattgatt ttcaagtgct tgggattggg
     8881 cgtaatgggc acattggttt taacgaacct gggacctctt ttgaagaaga aacgcatgta
     8941 gtggatcttc aggaatcaac tatcaaagcc aatagtcgtt tctttacaag tattgaggat
     9001 gtgccaaaac aagctatctc tatgggaatt gcctccatca tgaagtccaa aatgattgtt
     9061 cttcttgcct ttggacaaga aaaagctgat gcgatcaagg gaatggtttt tggaccaatc
     9121 acagaagacc tcccagcaag tattctccaa aaacatgatc atgttatcgt cattgtggat
     9181 gaagctgctg cgtcacaatt agattaacct aaccagtcgt taaaccttgt aagaacaaaa
     9241 atgaggagat agtcaacctc atttaagaag aagtcttgta acttctattt cttttgcaag
     9301 ggtaaattta tgttacacta ctaatatgcg tttagataaa ttattagaag gtacaaaagt
     9361 tggctctaga agtcaagtga agaaactgat taaggctcaa ggggtttggg ttgatcacat
     9421 gcctgccaga aatggtcgac aaaatgttga tcctggcttg caactgattg aggtgactgg
     9481 acagcgagtg acgcatccta aacattccta cattatcctc aacaagccat caggggtggt
     9541 ctcagctaag aaagatacta actatttaac cgttattgat cagcttgctg aagaagataa
     9601 aagtccagac ttgtatcctg ttggcagatt ggatagagat accgaagggt tggttttact
     9661 cactgataat ggtcccctag ggtttcggat gttgcaccct attcatcatg tttctaaaac
     9721 gtatcttgta accgttaatg gcttgttagc agaagatgcc tcagattttt ttgcagctgg
     9781 tatttgtttc ccaacaggtg aacaatgtca accagctcaa ttgactattc ttaaagccga
     9841 cacagaccag tcacaggcta gtttgaccat ttcagaagga aaatttcacc aagtgaaaaa
     9901 aatgtttttg acctatggtt tgaaagtgac ctctctgaaa cggacgcact ttgcaggatt
     9961 ggaactcggt catttagcca gcggagaata tcgttatcta acagcgtcag aaaggcaact
    10021 cattaaaacg tatctagact gagcaaaaca tgctttcaaa aagaagctat tcttgcttaa
    10081 tcaataaagt gtccattgac tattatagtc atgatgagtc cgacttttgc tatgtttagt
    10141 catcttgttg ttacaaaatc aggtaagcaa aaaaattcaa ttcccactgt ctccttaacc
    10201 tgaagcgtta taaaaaccct tgtgttaaac tctcataaat tggaaatggg agtgtttttt
    10261 gatggctaaa aaaactaaag ctacttaaag tttggtactt agttttcgaa taataaggtg
    10321 gaagatctaa taggaggtga caaaaatatt aaccgcttta gatggtaaga atcagctcat
    10381 ttcgctagta actcagccta tttcaacgaa accgcctttt tgttgcccag cttgcaaatc
    10441 tcctgttcgc ttgcgtcagg ggacaattag acgacctcat tttgcccatg tacaactagc
    10501 tcattgtcaa ttccaagcag aaaatgaatc tgaggaacat ttgacgttga aggcaaaact
    10561 ttacaccagt ttagtccgaa cagaagctgt ctgtattgaa aaatatcttc ctgagctgca
    10621 acagatagca gacctttggg tgaatgataa gttagcctta gaaattcagt gcagtccctt
    10681 gccagtggag cgtctaaaga aacgcaccaa agcctatcaa gaaaaggggt atccggtacg
    10741 atggttactg ggaagaaaat tgtggcttaa cactcactta actgctttgc aaaaacagtt
    10801 tctttacttt tcatctagtt tgggctttca tttgtgggag ctggatgcag ctgcaaatct
    10861 gctacgtctc aaatatttaa tccatgaaga cttatttggt aaggtaagtt atctaaccaa
    10921 aaccatttct ttagaccaca atattatgga aatgtttcga ttaccctatc agcaagaaat
    10981 cctttattct tatcagaaaa aaatgactgt gaacctatct aaaagaatac aaagagctct
    11041 tttagctaga cacccgaaat ggctacgacg acaagaaaag gcttacttat caggctataa
    11101 tttacttatg ttaacaactg atgcttttta tcctcaatgg agaccagttc aatcttccag
    11161 cggtttttgt caaattaaag gaaatcttcg cccttattat gaaagtttta aagtctatta
    11221 taaaaaagaa aaggataaga aggtgcaaac gcttttttca ccaaaatatt atgttaaaat
    11281 ggatagtaat agaaaatgag aaggagatgt tatgactgat aatcgcagcc atttagaaga
    11341 aaaatacact tgggatttga gcactatttt tgcaacagat aaggattggg aagctgaagt
    11401 cagtgactta gctactgaaa ttgacgcggc taaggatttt tcaggtcatt tactagattc
    11461 tagcgctaat ctgttgacaa ttacagatac ttatttagag ttagctcgtc gtgttgagaa
    11521 agtctatgtt tatgctcaca tgaaaaatga ccaagacact actgttgcca agtatcaaga
    11581 ataccaagct aaggcttcag gcttgtatgc aaagtttagc gaagtgtttt cattctatga
    11641 cccagaagtt atggcattga gtcaggaaga ttaccaagct tttcttgcgg aaacacctga
    11701 tttaaaaagg tatcatcatt tctttgacaa attgttccag acacgtgacc atgtgctcag
    11761 ccaggctgaa gaggaattat tagcaggagc ccaagaaatt ttcaacggcg ctgaagaaac
    11821 ctttagtatt cttgataacg ctgatattgt ctttccagta gttaaaaatg ataaggggga
    11881 agatgttgaa ttaacccatg gtaacttcat tagcctaatg gaatctaagg accgtagcat
    11941 tcgccaagct gcttatgaag ccatgtatag tacttatgaa cagttccaac atacttacgc
    12001 taaaaccttg caaaccaatg tgaaagtgca aaactataag gcgcgtgttc ataaatacga
    12061 ttcagcacgt caagctgcca tggctgctaa ctttattcca gaagctgttt acgatacctt
    12121 gttagaaacg gttaataaac atttgccact cttacaccgt tatttgaaat tgcgtcaaga
    12181 ggttcttggt ttagacgatt tgaagatgta tgatgtttac acaccattat ctgaaacaga
    12241 tttagccatt ggttatgatg aggctttgga aaaggctgag aaggttttgg ctgtttttgg
    12301 taaggattac gcggatcgtg tacatcgtgc ttttaccgaa cgttggattg atgttcacgt
    12361 caataaaggg aagcgctctg gggcttattc aggtggttcc tatgatacca atgccttcat
    12421 gctgttgaac tggcaagata cacttgataa tctttacaca cttgtccacg aaacaggcca
    12481 cagtttgcat tctactttca ctcgtgaaac acagccttat gtgtatgggg attatagtat
    12541 cttcttggca gaaattgcat ccacaacgaa tgaaaacatc atgacagaag ctctcttaaa
    12601 tgaagtgcaa gatgagaaag aacgctttgc cattttaaac cattacctcg atggtttccg
    12661 tggaacagtt ttccgtcaaa cccaatttgc tgagtttgaa catgccattc atcaagctga
    12721 tcaaaaagga gaagtgttaa caagtgaata ccttaaccag ctatacgctg atttaaatga
    12781 aaaatattat ggtttaagta aaaaagataa tcattttatt cagtatgaat gggcacgtat
    12841 tcctcatttc tattacaatt attatgttta ccaatatgca acagggtttg cagctgcaag
    12901 ctatttagcg gataagattg tgcatggcac acaagatgat atcgatcatt acttggccta
    12961 cctcaagtca ggaaattctg attacccact tgaagtgatt gctaaagcag gcgttgatat
    13021 ggcaaaaggt gattatttgg aagctgcctt taaagtcttt gacgaacgtc taacagaact
    13081 tgaagtactc gtgtccaaag gcatacacaa aagctaaaat cacaaaagtc aaggacaaaa
    13141 agagtttagg attgatagaa atggagattc acagttggtg tgaatctcca tttctatcgg
    13201 tcattttctt tttatatttt agtggattat cagtcctgaa aagcaggctt cattgacctt
    13261 agtttgacgg tttctttttt gtctttttta ttgtgtggtc actcacttaa ataaaaccaa
    13321 tagttgagta aggcgcttca gattaacgcc attccattgt tttagagata
//
LOCUS       DATUYR010000041        13284 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.041, whole genome shotgun sequence.
ACCESSION   DATUYR010000041 DATUYR010000000
VERSION     DATUYR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13284
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.041"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..669)
                     /locus_tag="VCI33_001196"
     CDS             complement(<1..669)
                     /locus_tag="VCI33_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832123.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNIN
                     TSADS"
     gene            complement(679..1377)
                     /locus_tag="VCI33_001197"
     CDS             complement(679..1377)
                     /locus_tag="VCI33_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832124.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(1377..1748)
                     /locus_tag="VCI33_001198"
     CDS             complement(1377..1748)
                     /locus_tag="VCI33_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2832125.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            1933..5043
                     /locus_tag="VCI33_001199"
     CDS             1933..5043
                     /locus_tag="VCI33_001199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEP2832126.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLRELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQIYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSNYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIVEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKIMDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            5123..6136
                     /gene="pfkA"
                     /locus_tag="VCI33_001200"
     CDS             5123..6136
                     /gene="pfkA"
                     /locus_tag="VCI33_001200"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEP2832127.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            6199..7701
                     /gene="pyk"
                     /locus_tag="VCI33_001201"
     CDS             6199..7701
                     /gene="pyk"
                     /locus_tag="VCI33_001201"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEP2832128.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICCETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            7919..8476
                     /gene="lepB"
                     /locus_tag="VCI33_001202"
     CDS             7919..8476
                     /gene="lepB"
                     /locus_tag="VCI33_001202"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP2832129.1"
                     /translation="MVKRDFIRNILLLLIVIIGAILLRIFVFSTFKVSPETANTYLKS
                     GDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYL
                     EKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLI
                     NASQIKGLVTFRVLPLSDFGFVEVE"
     gene            8652..10466
                     /gene="glmS"
                     /locus_tag="VCI33_001203"
     CDS             8652..10466
                     /gene="glmS"
                     /locus_tag="VCI33_001203"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEP2832130.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            10662..10997
                     /locus_tag="VCI33_001204"
     CDS             10662..10997
                     /locus_tag="VCI33_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEP2832131.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            11104..11745
                     /locus_tag="VCI33_001205"
     CDS             11104..11745
                     /locus_tag="VCI33_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP2832132.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            11755..12384
                     /locus_tag="VCI33_001206"
     CDS             11755..12384
                     /locus_tag="VCI33_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP2832133.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKSDAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            12400..13236
                     /locus_tag="VCI33_001207"
     CDS             12400..13236
                     /locus_tag="VCI33_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP2832134.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKGLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQEKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
BASE COUNT         4178 a         2201 c         2770 g         4135 t
ORIGIN      
        1 actatcggca cttgtattaa tattaaagat gtgactgtga acatatgttg cagcaacgct
       61 aatcagaata actaatataa aatatgcaat aaaggccatc aagcctcgcc gattagaaaa
      121 aagttgacct atagaaatgg aaagataaat taataagata ctcgtaaatg ttgataatag
      181 gacataggct agtacagtta gcatattgat gaaataatcc atcttaaaac tattaaagaa
      241 tccttctaat agttcgttga tgttaaacat tggtacaatt acaatagcga tagcaaatgc
      301 aaggatgata gtattgaaaa cactgcaaat aaaagaagct agtagttttg ataaaataat
      361 ttgatgcgaa ttaacgggta aagtcaacgt taagtatcct tcccgtccaa aaacactttt
      421 gctaaaacgt ttgataatga taagtaatgt tgataagagg gaaccagcga tcagggagcc
      481 aaaagttaaa cctaatgtca gaggtaacat cttattggtt agtactccaa atagtccatc
      541 actattactt tgagcaaaca gttttatcgt aaatgacaaa atggcagcga tggcaataac
      601 gaaagcatta agcgcaaagt accattttcc aatagatcta aattcgtatt ttaataattt
      661 tccaaacatg atatcctcct aaaccttata agtttcccta aagagggaat caatggattg
      721 ctggtattct tgtcttagat catcagcatt gccagataag aaaagtcttc cttgtttcaa
      781 gaaaatgact tcgtcaagga taggttcaat gtccgaaatc aagtgggttg agataataac
      841 agaagcattt tcacaataac tattaatgat ggtttttagg atgtagtctc ttgcggcagg
      901 gtcaactcca ccaataggtt catcaagtac atagagtctg gctttacgac tcatcacaag
      961 aattaattgt actttttctt tgtttccttt ggatagagtt ttgaagcggt cttcagggtc
     1021 tagttctaaa tctcttagaa ggcttgttgc tttggatttg tcaaagtcgc tgtaaaaatc
     1081 actaaagaac tccaacatgt ccttaattct catattttca ttgagataag tagtatctgg
     1141 taaataagaa ataatttttt ttgtctcaac acagggacgg tagccatcaa tgacaatttc
     1201 tcctttattt ggttgtaaaa ggccattaat gagtttaatt aaagttgttt taccacttcc
     1261 atttggacct aataatccga taattttacc gttcgggata gtaatggtta agtcatcaat
     1321 agcttttttt tcacggtaag attttgaaac atgatgtagt tgcaataggt gtgccattat
     1381 tgtgaatcct cctttaaaaa gctcgtcaaa acagggataa tctcagtgtg actaaatccc
     1441 atttttgtca tattggtgat aaatgattct aattcactaa ttgctaactc tcgtctttta
     1501 cgagcaatta atttttgatc gtcagttaca aaacggccag cagtacgttg cgaataaacc
     1561 attccttccc gttcaagttc tgtaaacgct cgttgcatgg tgttaggatt aacacctgcg
     1621 atttctgcat attcacgaac agttggaagt tgatcaccac tcttgatttc ttggctaata
     1681 atttgcatca tgacatgttg tgctatctgc gcataaatag gagatttttc atcaaatttc
     1741 caagacatgt ggtctccttt ctgagtaata tagatatttg tactaattaa atggtacaga
     1801 aaaaaaaagg gaatgtcaag ttttatgcca tttttcactc tttgagaagt agacgtagat
     1861 tagagagaaa attgaagcaa gaaaaccttt ttcatagtat aatagggaac aagataacca
     1921 aaggaggaac ttatgtttgc tcaacttgat actaaaactg tatactcatt tatggatagt
     1981 ttaattgact taaatcatta ttttgaacga gcaaagcaat ttggttacca caccatagga
     2041 atcatggata aggataatct ttatggtgct taccatttta ttaaaggttg tcaaaaaaat
     2101 ggactgcagc cagttttagg tttggaagta gagattctct atcaagagcg gcaggtgctc
     2161 cttaacttaa tcgcccagaa tacacaaggc tatcatcagc ttttaaaaat ttccacggca
     2221 aaaatgtctg gcaagcttca tatggattac ctctgccaac atttggaagg gatagcggtt
     2281 attattccta gtaagggttg gagcgataca ttagtggtcc cttttgacta ctatatcggt
     2341 gttgatcagt atactgattt atctcatatg gattctaaga ggcagcttat acccctaagg
     2401 acagttcgtt attttgcgca agatgatatg gaaaccttgc acatgttgca tgccattcga
     2461 gataacctca gtctggcaga gacccctgtg gtagaaagtg atcaagagtt agcagattgt
     2521 caacaactaa ccaccttcta tcaaacacac tgccctcaag ctctacagaa tttagaagac
     2581 ttagtgtcag gaatctatta tgatttcgat acaaatttaa aattgccaca ttttaataga
     2641 gataagtctg ccaagcaaga attgcaagac ttgactgagg ctggtttgaa ggaaaaagga
     2701 ttgtggaaag agccttatca atcgcgctta ctgcgtgaat tggtcattat ttctgacatg
     2761 ggctttgatg attatttttt gattgtgtgg gatttacttc gctttggacg cagtaaaggc
     2821 tattatatgg gtatgggacg tggctcggcg gcaggtagtc tagtggctta tgctctgaac
     2881 attacaggga ttgatccagt tcaacatgat ttgctatttg agcgcttttt aaacaaagaa
     2941 cgttatagca tgcctgatat tgatatcgat cttccagata tttaccgttc agaatttcta
     3001 cggtatgtcc gaaatcgtta tggtagcgac cattcggcgc aaattgtgac cttttcaacc
     3061 tttggcccta aacaggctat tcgtgatgtt ttcaaacggt tcggggttcc agaatacgaa
     3121 ctgactaatc tcactaaaaa aattggtttt aaagatagct tggctactgt ctatgaaaag
     3181 tcaatctctt ttaggcaggt tattaatagt agaactgaat ttcaaaaggc ctttgctatt
     3241 gccaagcgta tcgaaggaaa tccaagacaa acgtccattc acgcagctgg tattgtgatg
     3301 agtgatgata ccttgaccaa tcatattcct ctaaaatcgg gcgatgacat gatgatcacc
     3361 cagtatgatg ctcatgcggt cgaagctaat ggcctgttaa aaatggattt tttggggtta
     3421 agaaatttga cctttgttca aaaaatgcaa gagaaggttg ctaaagacta cgggtgtcag
     3481 atcgatattg cagccattga tttagaagac ccgcaaacgt tggcactttt tgctaaaggg
     3541 gataccaagg gaattttcca atttgaacaa aatggtgcta ttaatctttt aaaacggatt
     3601 aagccacaac gttttgaaga aattgttgcc actaccagtc taaatagacc aggggcaagt
     3661 gactatacca ctaatttcat taaacgaaga gaaggacaag aaaaaattga tttgattgat
     3721 cctgtgattg ctcccatttt agagccaact tacggtatta tgctttatca agaacaagtt
     3781 atgcagattg cacagattta tgctggtttt acgttaggca aggccgactt gttaaggcgt
     3841 gccatgtcta aaaaaaatct acaagaaatg caaaaaatgg aagaagactt tattgcttct
     3901 gctaagcacc tagggagagc tgaagaaaca gctagaggac tttttaaacg gatggaaaaa
     3961 tttgcaggtt atggttttaa ccgcagccat gcctttgcct attcagcttt agcttttcaa
     4021 ttggcttatt tcaaagccca ttacccggct gttttttacg atatcatgat gaattattct
     4081 agcagtaact atatcacaga tgctctagaa tcagattttc aagtagcgca agttaccatt
     4141 aatagtattc cttacactga taaaattgaa gctagcaaga tttacatggg gctgaaaaat
     4201 attaaggggt tgccaaggga ttttgcttat tggattgttg agcaaagacc atttaatagc
     4261 gtagaggatt ttctcactag aactccagaa aaatatcaaa aaaaggtttt ccttgagcct
     4321 ctgataaaaa taggtctgtt tgattgcttt gagcctaacc gtaaaaaaat tatggacaat
     4381 ttggatggtt tactggtatt tgttaatgag cttggttctc ttttttcaga ttcgtccttt
     4441 agttgggtag atacgaaaga ttactcagca actgaaaaat attctttgga acaggagatc
     4501 gttggggttg gcatgagcaa gcatccttta attgatattg ctgagaaaag tacccaaact
     4561 tttactccca tttcacagtt agtcaaagaa agcgaagcag tcgtactgat tcaaatagat
     4621 agcattagga tcattagaac caaaacaagt gggcagcaaa tggctttttt aagtgtgaat
     4681 gacactaaga aaaagctcga tgtcacactt tttccacaag agtatgctat ttataaagac
     4741 caattaaaag aaggagaatt ctattactta aaaggtagaa taaaagaaag agaccatcga
     4801 ctgcagatgg tgtgtcagca agtgcaaatg gctattagtc aaaaatattg gttattagtt
     4861 gaaaaccatc agtttgattc ccaaatttct gagattttag gtgcctttcc aggaacgact
     4921 ccagttgtta ttcactatca aaaaaataag gaaacaattg cattaactaa gattcaggtt
     4981 catgtaacag agaatttaaa ggaaaaactt cgtccttttg ttctgaaaac ggtttttcga
     5041 taaaaatgga caattagtga agtattttgc aaatgaatgt gctataataa gaccgttaaa
     5101 ttaattaaag gagtaccaga aaatgaaacg tattgctgtt ttaaccagtg gcggcgacgc
     5161 tcctggtatg aacgctgcta ttcgtgcagt cgttcgtaaa gcaatttcag aaggaatgga
     5221 agtttatggt atcaaccgtg gctatgcagg tatggttgat ggcgacattt tcccactggg
     5281 ttcaaaagaa gttggtgata aaatctcacg tggtggaact ttcttatact cagctcgtta
     5341 tccagagttt gctcaacttg aaggtcaatt ggctggtatt gagcaactta aaaaacatgg
     5401 tattgaaggt gttgttgtta ttggtgggga tggttcttat catggcgcta tgcgcttgac
     5461 agagcacggc ttcccagcgg taggtatccc aggaacaatc gataacgata ttgcgggaac
     5521 tgactacact atcggatttg atacagcagt taacacagct gttgaagcta ttgataaact
     5581 tcgtgacacc tcatcaagtc acggacgtac ttttgtggtt gaagtgatgg gtcgcaatgc
     5641 gggtgatatt gctctttggg caggtattgc tagtggtgct gatcaaatca ttgttccaga
     5701 agaagagttt gatattgaaa aagtggcatc aacaatccaa tatgattttg aacacaaagg
     5761 taaaaatcac cacattatcg tcttagctga aggtgttatg agcggtgaag cttttgctca
     5821 aaaactgaaa gaagctggcg ataagagtga ccttcgtgtg acaaatcttg gccacattct
     5881 tcgtgggggt tcaccaactg ctcgtgaccg tgtcattgct tcttggatgg gttcacatgc
     5941 tgttgagcta cttaaagaag gcaaaggtgg tttggctgtt ggtattcaca atgaagaact
     6001 tgttgaaagc ccaatccttg gtactgcaga agaaggtgct ttgttcagct tgactgaaga
     6061 aggcaagatt attgttaata atccacacaa agctcgttta gactttgcag ctttgaaccg
     6121 ttcattgtca caataagttt agttaaaatt gttaaatcgt caattattga caaaaaaata
     6181 agggagtttc agagtaaaat gaataaacgc gtaaaaatcg ttgcaacact tggtcctgcg
     6241 gttgaaatcc gtggtggtaa aaaatacgga gaagatggct actgggctgg tcaacttgat
     6301 gttgaagaat ctgcaaaaaa aatcgctgaa ttaattgaag ctggtgcgaa tgttttccgt
     6361 ttcaatttct cacatggtga ccacaaagaa caaggtgacc gtatggcaac tgtccgcctt
     6421 gcagaagaaa ttgctcgtca aaaagttggt ttccttttgg atactaaagg tcctgaaatg
     6481 cgtacggaat tgtttgcaga tgatgctaaa gagttcagct atgttactgg agaaaaaatc
     6541 cgtgtagcga ctacacaagg tattcaatca actcgtgatg ttattgcttt gaacgttgct
     6601 ggtagccttg acatctacga tgaagtagaa gtaggtcata ctattcttat cgatgatggt
     6661 aaacttggtt tgaaagtgat cgacaaagat atcgcaacac gtcaatttat cgttgaagta
     6721 gaaaacgacg gtattattgc taaacaaaaa ggtgttaata tccctaacac taaaattcct
     6781 ttcccagcac ttgcggaacg cgacaatgct gatatccgtt ttggtcttga acaaggactt
     6841 aactttattg caatctcatt tgttcgtaca gccaaagacg ttgaggaagt tcgtgaaatc
     6901 tgttgcgaaa ctggtaacga ccatgtgcaa ttatttgcta agattgaaaa ccaacaaggt
     6961 atcgataatc ttgatgaaat catcgaagct gctgatggta tcatgattgc acgtggcgac
     7021 atgggtatcg aagtaccatt tgaaatggtt ccagttttcc aaaaaatgat tatcactaaa
     7081 gtgaatgcag ctggtaaagc agttatcaca gcaacaaaca tgcttgaaac aatgactgaa
     7141 aaaccacgtg cgacacgttc tgaagtatct gacgtcttca atgctgttat cgatggtact
     7201 gatgcaacaa tgctttcagg agagtcagct aacggtaaat acccggttga atctgttcgt
     7261 acaatggcga ctattgatcg taatgcacaa actcttctta acgaatatgg tcgtttagat
     7321 tcatctgcct ttccacgtac caataaaaca gatgttattg cttcagctgt taaagatgcc
     7381 actcattcaa tggacatcaa acttgttgtg actattactg agacaggtaa tacagctcgt
     7441 gcgatttcta aattccgtcc agatgcggac atcttagctg taacatttga tgaaaaagta
     7501 caacgtgctt tgatgattaa ctggggtgtt attcctgttc ttgctgaaaa acctgcatct
     7561 actgatgaca tgtttgaagt agcagagcgt gttgctgtgg aggcaggctt agttcaatct
     7621 ggggataata ttgttattgt tgctggtgtt ccagttggta ctggtggaac gaatactatg
     7681 cgtgttcgta cagtaaaata aatgtgttat aactatttac ctaaaggtta gaaggcaagc
     7741 ttctaacctt ttttgatatc aaaaatatca tgttaattca ctagtgttaa tttactttaa
     7801 tgagtctaag tttgtatttt atacagaatg agaaacgttt attggtattg atcaaaaatt
     7861 gtttactttt tttctagctt agaacatggt ataataagac aatagactag gagttaatat
     7921 ggtaaagcga gattttataa gaaatatatt attgttatta attgtgatta ttggggctat
     7981 tttgttgaga atctttgttt tttcaacttt taaagtttct ccagaaacag ctaatactta
     8041 tttaaagagt ggtgatttag tcacaatcaa aaaaaatatt cagcccaaat ataaagattt
     8101 tgtggtttat agagttggaa aaaaagatta tgtcagtcga gtcattgctg ttgaaggcga
     8161 tagcgtgact tatatggacg atatttttta cctcaataat atggtagagt cacaggctta
     8221 ccttgaaaag atgaaagcac attacttgaa tcatgcaccg tttggcacat tgtatacaga
     8281 tgattttaca gttgctacca tcacagctga taagtatcaa aaagttccta aggggaagta
     8341 tcttcttttg aatgataacc ggaaaaatac gaatgacagt cgtcgatttg ggttaataaa
     8401 tgcctcgcag attaaaggtt tagtgacctt tagagttttg cctctcagcg attttggatt
     8461 tgtagaagta gagtagttgt caggctactc tacttttttt ataattagac tataccaatt
     8521 ttatgtttga caagacattg tggatattat atactgtaaa catactgttt ttatcggtat
     8581 aatatgacta taccaattat tgaaattatg agagaaagta gcaagttaac gaacttgtta
     8641 aggtaattaa gatgtgtgga attgttggag ttgttggaaa tcgcaatgca acggatattt
     8701 taatgcaagg ccttgaaaag cttgaatacc ggggttatga ttcagcagga atttttgtgg
     8761 ctaatgccaa tcaaacaaac ttgattaaat cagtggggcg gattgctgat ttgcgtgcca
     8821 agattggcat tgatgttgct ggttcaacag ggattggtca cacccgttgg gcaacgcatg
     8881 gccaatcaac agaggataat gcccatcctc acacgtcaca aactggacgt tttgtacttg
     8941 ttcataatgg tgtgattgaa aattaccttc acattaaaac agagttccta gctggacatg
     9001 attttaaggg gcagacagat actgagattg cagtacactt gattggaaaa tttgtggaag
     9061 aagacaagtt gtcagtactg gaagctttta aaaaagcttt aagcattatt gaaggttcct
     9121 acgcctttgc gttaatggat agccaagcaa ctgatactat ttatgtggct aaaaacaagt
     9181 ctccattgtt gattggactt ggtgaaggtt acaacatggt ttgttcagat gccatggcca
     9241 tgattcgtga aaccagtgaa tttatggaaa ttcatgataa ggagctagtt attttaacca
     9301 aagataaggt aactgttaca gactacgatg gtaaagagct gatacgagat tcctacactg
     9361 ctgaattaga cttatctgat attggcaaag ggacttatcc tttctatatg ctgaaagaaa
     9421 ttgatgagca accaaccgta atgcgtcaat taatttcaac ttatgcagat gaaactggta
     9481 acgtacaggt tgatccagct atcattacct ctatccaaga ggctgaccgt ctttatattt
     9541 tagcggcagg gacttcctac catgctggtt ttgcaacaaa aaatatgctt gagcaattga
     9601 cagatacacc agttgagttg ggagtggctt ctgagtgggg ttaccacatg cctctgctta
     9661 gcaagaaacc aatgtttatt ctactaagcc aatcaggaga aaccgcagat agtcgtcaag
     9721 ttttagtaaa ggcaaatgct atgggtattc cgagtttgac agtaactaac gttccaggat
     9781 caaccttatc acgtgaagca acatacacca tgttgattca tgctggacct gaaattgctg
     9841 ttgcgtctac aaaagcttac actgcacaaa ttgctgccct tgcctttttg gctaaggcag
     9901 ttggtgaggc aaatggtaag caagaagctc ttgactttaa cttggtacat gagttgtcat
     9961 tggttgccca atctattgag gcgactttgt ctgaaaaaga tctcgtggca gaaaaggttc
    10021 aagctttgct agctactact cgtaatgctt tttacattgg gcgtggcaat gattattacg
    10081 ttgcgatgga agctgctttg aaattaaaag agatttctta tattcaatgc gaaggctttg
    10141 cggctggtga attgaaacat ggaaccattt cattgattga ggaggacacg ccagtaatcg
    10201 ctttaatatc gtctagtcag ttggttgcct ctcatacgcg tggtaatatt caagaagttg
    10261 ctgcccgtgg ggctcatgtt ttaacagttg tggaagaagg gcttgaccgt gagggagatg
    10321 acattattgt caataaggtt catcctttcc tagccccgat tgctatggtc attccaactc
    10381 aactgattgc ttactacgct tcattacaac gtggacttga tgttgataag ccacgtaatt
    10441 tggctaaagc tgtaacagta gaataaatga ttataatgga taaggcaacc atctgttagg
    10501 tggttgcttt ttgtttttag gagaatagca gagcttagaa aatggccttg agaaaagcta
    10561 tacaagaaga atagtcatct tctgatgaga acgtattcat cttgtcaaat cattgattct
    10621 aaagtaaaat ggtaacatta agaaatgttt aggagtgtaa gatgtcatta ccaaattgtt
    10681 tgcaatgcca gtctgaatat gtttacgaag atggtctttt attaatctgt ccaatgtgtg
    10741 cttttgaatg gacaccaggc gaagaagcga ctgaagaaga aggacttgtt gttttagata
    10801 gcaatggtgt ccgactgtct gatggtgata ccattactgt ggttaaagat ttgaaagtaa
    10861 aaggcgctcc aaaagacctg aaacaaggga ctcgtgttaa aaatattcgc ctggtggaag
    10921 gtgatcataa cattgattgt aagattgatg ggtttggcgc gatgaaattg aaatcagaat
    10981 ttgtcaagaa aatctaataa taatacgcaa ccagttgatc tggttgtatt tttataaaaa
    11041 aacctctccc caaatacgtt caaatttggt ataatagtaa aatattcagt tttaggaggc
    11101 aacatgacct atattcagca agttttaccc agcttattag atggtgcctt ggtgacctta
    11161 caagtattct ttattgttat cattctttct atacccttag gggctatttt agctttcttg
    11221 atgaagattc cctttaaacc gctacagtgg tttttgaccc tatacgtgtg gatgatgcga
    11281 gggacaccat tactacttca attgattttt ttctattatg ttttgccaag tgtggggatt
    11341 agttttgatc gaatgccagc tgctattttg gcgtttactt tgaattatgc tgcctacttt
    11401 gctgaaattt ttagaggtgg gattgaggct attccaaaag gtcaatatga agcagctaaa
    11461 gtattaaagt taaaacctct tcaaaccatt cgttatatta ttttgcctca agtgtttaaa
    11521 attgtgttac caagtgtttt caatgaagtc attaatttgg tcaaagattc ttcccttgtc
    11581 tatgtactcg gtgtaggaga tcttttatta gcaagtaaga cggcagccaa tagggatgca
    11641 accttagccc ctatgtttat cgctggtctt atctatttgc ttttaattgg actggtcacg
    11701 attatttcaa aacaagttga aaaacggttt aattattatc agtaaggagg cttcatgtta
    11761 gaactcaaaa atatttccaa acagtttggt caaaaaacca tttttgatgg ctttaatcta
    11821 accgttcaag acggagaagt gctctctttg gtaggcccat caggtggcgg taagacaaca
    11881 ctcttacgga tgttggctgg tcttgaatct attgattcag gtcaagtctt ttacaatggc
    11941 gaagatgttg gtattgatca tttggaaaat cgtaacttgc ttggatttgt ttttcaagat
    12001 tttcaattgt ttcctcatct taccgtttta gacaacctaa cgctgtctcc aacaattaca
    12061 atgggcaagc aaaaatctga cgctaaagaa aaggctttgg atttactggc acgtttagga
    12121 ttaaaagaac atgcccaagt ttatccctat tcgttatctg gaggtcaaaa acaacgggtt
    12181 gctttggcaa gagccatgat gattgaccct caaattatcg gttacgatga accgaccagt
    12241 gctctagatc ctgaattacg tcagacggta gaagccttga ttgttcaaaa ccgtgaaatg
    12301 ggaataactc aaattgtagt cactcacgat cttgtttttg ccgaagccat ctcagatcgt
    12361 attattaggg ttaatcctaa atagaagagg acccaaaaga tgattataaa aaaaagaacc
    12421 gtagcaattt tagccatagc tagtagcttt ttcttggtag cttgtcaagc tactaaaggt
    12481 cttaaatcag gagatgcttg gggagtttac caaaagcaaa aaagtattac agttggtttt
    12541 gacaatacgt ttgttcctat gggctataag gatgaaagcg gcaaatgcaa aggttttgat
    12601 attgatttgg ctaaagaagt ttttcaccaa tatggactca aggttaactt tcaagctatt
    12661 aattgggaca tgaaagaagc agaactaaac aatggtaaaa ttgatgtaat ctggaatggt
    12721 tattcaataa ctaaggagcg tcaggaaaag gttgccttta ctgattctta catgagaaat
    12781 gaacaaatta ttgttgtcaa aaaaagatct gatattaaaa caatatcaga tatgaaacat
    12841 aaagtgttag gagcacaatc agcttcatca ggttatgact ccttgttaag aactcctaaa
    12901 ctgctgaaag attttattaa aaataaagac gctaatcaat atgaaacctt tacacaagct
    12961 tttattgatt taaaatcaga tcgtatcgat ggaatattga ttgacaaagt atatgccaat
    13021 tactatttag caaaagaagg gcaattggag aattatcgga tgatcccaac gacctttgaa
    13081 aatgaagcat tttcggttgg acttagaaaa gaagacaaaa cgttgcaagc aaaaattaat
    13141 cgtgctttca gggtgcttta tcaaaatggc aaatttcaag ctatttctga gaaatggttt
    13201 ggagatgatg ttgccactgc caatattaaa tcttaaaaag gacacttgtc ctttttttgt
    13261 ttaacaggtg agatctagca gaca
//
LOCUS       DATUYR010000042        13249 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.042, whole genome shotgun sequence.
ACCESSION   DATUYR010000042 DATUYR010000000
VERSION     DATUYR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.042"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..770)
                     /locus_tag="VCI33_001208"
     CDS             complement(<1..770)
                     /locus_tag="VCI33_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEP2832135.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEVSTAAM
                     TKEERQQEIEKIKEDIKKEYPKASEEKFWESSFWGRRYFNEQEYLKSLQSFTEQRLQD
                     IVNLIEKGATKGDKGDAGPKGERGPAGPQGPVGPKGEAGKVGAQGPKGDPGAPGKDGA
                     KGEKGDKGDTGERGEKGDIGATGAQGPQGEKGETGAQGPAGPQGEAGKPGEQGPAGPQ
                     GEAGKPGEKAPEKSPEGEAGQPGEKAPEKSPEVTPTPET"
     gene            1152..1634
                     /gene="nrdI"
                     /locus_tag="VCI33_001209"
     CDS             1152..1634
                     /gene="nrdI"
                     /locus_tag="VCI33_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP2832136.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(2028..2846)
                     /locus_tag="VCI33_001210"
     CDS             complement(2028..2846)
                     /locus_tag="VCI33_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEP2832137.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPNIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLGWR"
     gene            complement(2929..5115)
                     /locus_tag="VCI33_001211"
     CDS             complement(2929..5115)
                     /locus_tag="VCI33_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP2832138.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHESAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGALASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASLAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDHNTQATPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKIGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGAVIPEVNMSQLT
                     SKPTPAKDFKHVTEEVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTTEIQDVTLTSLGAQPAKTKVATVEL"
     gene            complement(5472..6221)
                     /locus_tag="VCI33_001212"
     CDS             complement(5472..6221)
                     /locus_tag="VCI33_001212"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEP2832139.1"
                     /translation="MQQYFIKGKAEKEVTITDKDTIKHMFQVMRLADKAEVVLVFDDG
                     VKYLAKVTNSMAHELEIIEALPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKAEFLKSLSG
                     FDHIFVAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPTEITQFEAAGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(6221..7174)
                     /locus_tag="VCI33_001213"
     CDS             complement(6221..7174)
                     /locus_tag="VCI33_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEP2832140.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAIIAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQILRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLIKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            complement(7244..7714)
                     /locus_tag="VCI33_001214"
     CDS             complement(7244..7714)
                     /locus_tag="VCI33_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEP2832141.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKVDFGLEWNAQAFEEGQQGLEEAASYAYPRY"
     gene            7915..9672
                     /gene="pabB"
                     /locus_tag="VCI33_001215"
     CDS             7915..9672
                     /gene="pabB"
                     /locus_tag="VCI33_001215"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEP2832142.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKSLDQVGPVIENVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDGITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDDFAVISASPELFFKQEGNRLTTRPMKGTTKRGVNSWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQTGSVCVDRLCEVERYSTVWQMTSTIVGDVKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVKHKKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKDNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFVQLGRTLYTPPVVVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLKLTHLDALLAKSQA"
     gene            9705..10271
                     /locus_tag="VCI33_001216"
     CDS             9705..10271
                     /locus_tag="VCI33_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEP2832143.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVLSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGNLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            10304..11572
                     /locus_tag="VCI33_001217"
     CDS             10304..11572
                     /locus_tag="VCI33_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEP2832144.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            11661..11857
                     /gene="ssrS"
                     /locus_tag="VCI33_001218"
     ncRNA           11661..11857
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VCI33_001218"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11858..11930
                     /locus_tag="VCI33_001219"
     tRNA            11858..11930
                     /locus_tag="VCI33_001219"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11891..11893,aa:Lys,seq:ctt)
     gene            12071..12511
                     /locus_tag="VCI33_001220"
     CDS             12071..12511
                     /locus_tag="VCI33_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2832145.1"
                     /translation="MKIIEYKGNYRDDLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            12566..13072
                     /locus_tag="VCI33_001221"
     CDS             12566..13072
                     /locus_tag="VCI33_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEP2832146.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVVHLDQLHFSSNWQERS
                     DHDMIADLSTCLLKQDWIIEGNYANCLYEERMSEANHIIYVNFSRFHCVYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVIQKYPHKTIVLTNQKQL
                     NHYMNTIN"
BASE COUNT         3691 a         3087 c         2611 g         3860 t
ORIGIN      
        1 gtttctggcg ttggtgtcac ttctgggctc ttttctggag ctttttcgcc tggttggcct
       61 gcttcgcctt ctgggctctt ttctggagct ttttcacctg gtttgcctgc ttcaccttgt
      121 gggcctgctg gaccttgctc acctggtttg cctgcttcac cttgtgggcc tgctgggcct
      181 tgtgcgccag tttcaccttt ttcaccttgt gggccttgtg cgccagttgc tccgatgtca
      241 cctttttcac cgcgctcacc ggtatcacct ttgtcgcctt tttcaccttt ggcgccgtct
      301 ttacctgggg caccaggatc acctttagga ccttgggcac caaccttacc tgcttcacct
      361 ttaggtccta ccggaccttg tgggcctgct gggccgcgct cgcctttagg accagcatcg
      421 cctttgtcac cttttgttgc acctttttca atcagattta caatatcttg gagtctttgc
      481 tcagtaaaac tttgtaaaga ttttagatac tcttgttcat taaagtacct tcttccccaa
      541 aagctactct cccaaaattt ttcctcagac gcttttggat attctttctt aatgtcttct
      601 ttaattttct caatttcttg ttgtctctcc tccttcgtca tagccgcagt agaaacctca
      661 gccttaacgc tgcttgcccc gcctccaaag gctagaagga cggcagcggc tgaggttagc
      721 ccgtagcgcc agactagttt gttgagattg tggtgttttg atgtcaacat atgttgttct
      781 ctctttcttt attagatggt gtgttttaga taaaaataac atggtagcta gacctgatta
      841 tttataatag tattataata gagtgtgaaa acgattgtaa agttctcttt tgttgttcat
      901 cttttagatt ttgactgatc actcttttgt ggagatcaga gggctacttt ggcacttgcc
      961 tgcctcgttt ttaagtcgag ggcatccgga tcctttcttt aaggtcactc ttttttgaaa
     1021 tgttactttt tttgaccgct tgtaccttct tgtctggttg gatatcgcta ggtttcttct
     1081 ttacttgttt gttggtcttg attgtggtaa gcttatgaag aaaccttgta ggataaaaaa
     1141 aggagacact tatgcctcag attactcttg tttttattag cctcagtggc aacaccctta
     1201 gctttgtcaa gcggctatcc ctttatttag cagacaatta cgattaccat gtcaaacaga
     1261 ttaacattaa agatttgaag cacgagactt ttcccgtcaa ggaagagttt gtggcgattc
     1321 tacccactta tctagaaggc ggaaatggcg ttgactctgg tgaggtggag atcctcacca
     1381 ctcctttagg agagtttatt gccgcccatg gcaacgccca gcgttgtctt gggattattg
     1441 gttcaggcaa caaaaacttt aaccatcagt attgcctgac agccaaacag tatgccaaac
     1501 gctttggctt tccactgcta ggtgattttg agctgcgagg gactcctgat gacatttcgc
     1561 ggttagccca ggtcatcatg gaggccagct ccagacactc gtctaacgac acacagacac
     1621 tgcctaacag ctagaccgtc tatagatgat catagctgtt ttaaaaaaga gcacttttag
     1681 gacaccaact agggaggtct cttaccctac gccttgcgat ttctcagcct ttgaaaagag
     1741 cgatcacagt gcttcttggg gcttgggatc actccaagcg ctcctagaaa tacccctatg
     1801 cagactttag caaggcaact gaacgcttgg cggtactttc tgtgaagctc agatggttgg
     1861 tggcaaaaaa gccctggttt tttagggtta ctgacatgac taaagaggtc taaccagctc
     1921 taccttgagc tcgttagacc tctttgtgtt ctcctaatcc ccctagacga tcgcagtttc
     1981 ttatgtgtct tggcttctcc tatgtggtgt gcctgttagc acatgctcta acgccagcct
     2041 aatgtcactt ctaaaccgta gtggtcactg acgactggcg catctccgcc ttcaaaggta
     2101 atcttagaag atcggatgat gaagtccttg ctggtaaaga catggtccac cttgagggct
     2161 tctttgttac cttgccagcc atcaatatca gccacgatgc tatgatcacc aaacacatgg
     2221 tcagcaacct tatggctatc ttgtaggtct agggggctac ccatcatcac ttgataaccg
     2281 tcttgatccg ttggattgtt aaaatctccc ataagtagca agggacagtt tagtgtgagc
     2341 agttcttttt ctaatttctc ccactcccct aaaaaccctt tgtcaaacca agataggtgc
     2401 acattgacaa cagcgacttc tttgccatct aacgtggttt tggctatgag tgagcgtctg
     2461 gtgtggtagt ctgtttcatc agccatcgct gacacgagaa tatctgaaac atgaataggt
     2521 tgttttgata agattgccac gccttcctgg taaatgtcat agccaatatg gttataagcc
     2581 caagaccaat aatagtgctg ccctcgtttt tgtaaataat ggatcaagag cagggcaaaa
     2641 tggtctttat gaatgtttgg tgtccctgaa agagcttgat atcttggtaa atctgtggcc
     2701 agctcgctct cgattaactg attgatttct tgcaagcaaa tgatatcgta tttttcagct
     2761 aaaatatgct cagccaaagc aactaatttt ttaagagtgt tggcttgcat ccacgaatgg
     2821 gtattcagtg ttaatacctt ggtcattaat cctcctttat aagacaagaa gagccatagg
     2881 gcctaaacca gctatggcta ttacctatca ctatctcgtg atgttttgtt aaagttctac
     2941 ggtagctact ttagtcttag caggctgagc tcctaaagat gtcaaggtca catcttggat
     3001 ctctgttgtg ttggtaaaag caacaacaat ggttgtttca cgctctgctg atttaatagc
     3061 agcaagatct gctacgacta ataggtcacc tgcatgaacc cgttgtcctt ctgagacttt
     3121 gactgaaaaa ggcacaccat ttaaggcgac ggtatcaagt cccacgtgca ccagcacttc
     3181 aagaccatta tctgtcagta aaccaaaggc atgcttggtc ggaaagacac tggtcacaag
     3241 gccagctact ggtgcataga tattaccatg ggttggttca accgcaaacc catctcccat
     3301 catcttagcc gcaaacacct ggtctttcac acctgtgatt gggagaacca tcccgtctgc
     3361 cactgatagc acttcttctg tcacgtgttt aaaatctttt gcgggagtcg gtttgctagt
     3421 gagctgtgac atgttgactt ctggaataac agcccctgag tccagcaaat cttgaatatc
     3481 tgatttcaaa atatctgctt taggcccata gaccgcttga acgccgttgc ctttttggat
     3541 caagcccata gcgccagctt ttttccaatc gtcctcagcg ccaatcttag cggggtcttt
     3601 gaccgtcact cgtaagcgcg tcatacaagc atcaacatca tcaatattgc ctgccccacc
     3661 aaggagatta atgatttgta cgacttgaga gttgctatct gctacttggg taggtgttgc
     3721 ttgagtgtta tggtcaccca gtatatcggc atcgtagttg cctaaacggc cagctgtcgc
     3781 gagatgcatt ttcttgatca tcatatccgc aataaagtac atgataactg caaagaggat
     3841 agaaacccaa acaaagttaa tcacatccat gcctagacct gctttaaggg ccataggggt
     3901 acgggttagt agctcaatgt tcccaaatga gtgaacacgg agattcacaa ggtctgccat
     3961 ggcaagtgaa gctccttgaa caagggcata gacgatgtaa agtggcattg ctgcaaacat
     4021 aaataggtac tcaagtggct cagttacccc tgtcaaaaag accgctgccg ctgctgagat
     4081 gaacatcatt ttgtatgtat gttttttatc ggcatccaca ttacggtaca tggctaaggc
     4141 aacccccatc aatgttccgg tagctccaat catttgtcct accttaaagc gagctggagt
     4201 cacactatcc attaagtgac tataggctga agcgtctgat cctttaaggt gaacaaggtc
     4261 agttacccaa gcaagccaca aagggtcttg accaaatact tttgtccccg ctgctgcacc
     4321 tgtcatgacc tcataagttc caccaagagc tgtatagttc attggaatcg tcaacatgtg
     4381 gtgaagacca aatggcaaca agagacgttc caaggtacca tataggaatg gggctaggat
     4441 tggagctgaa tcttgtgaag aggcaatcca catcccaaaa ctgttaatcc cagactggat
     4501 caccggccaa acaacaacca aaataagggc tacaaaaata gaacgtaaaa taacgacaaa
     4561 cggtacaaaa cgcttcccat taaagaaggt caaaacttca ggaagtttac ggtaattata
     4621 gtatttgtta taggccgttg ctcctacgaa ccctgcaata atcccaacaa aaacccctgt
     4681 gtttaaagct ggagactcca agacactggt gaaataatct ttaacgatca tttgagtgcc
     4741 aaggaggctt gtgatttttg cttctggatc agctagcata gcactagaca cgccataaaa
     4801 agcacctgtt atccgattga tcaagacaaa agcaagccct gaggcaaggg cccctccagc
     4861 tctttcttta gcccaactac caccaatggc caaggcaaat agcaaatgaa ggttaacaat
     4921 aacagcccaa ccaatttgag cgataatgtt gcctagagat gccaaaaatg ctgattcgtg
     4981 gttaatcatt ggaatagagt ttccgatact gatcattaaa ccagccgctg gcatgacagc
     5041 aataacgacc ataagacatt tcccaaattt ttgccaaaat tcgaaacgaa acagttgctt
     5101 gaatgatgtt ttcattctat ctctccttta ttatcccctt ttagccatag ctgcccttaa
     5161 ggcgcttttg tgacacaatg atatagactg caactcagtg atgcaatcgt ttgcattgat
     5221 ctgatacctt tattatacta gcctcgcaac aatttgtaaa gcgttttctt agacagatgt
     5281 tagtcgtttt tcttcattaa atggtagtgt aacttaccaa aaagataaca agagcccatt
     5341 tccttttttg gtgatgagac catcaacgct cttcgaaaaa acaagaagca ataaaaaaag
     5401 gccgttgaca caatcgtgaa ctgcctgttc gtttctttag cctcttcttt ctaaagtaag
     5461 cagcagctta cttattttga tgcctgcaat tccaaagcat aactaagagc acttaaggca
     5521 tatagcggcg ctgtctctgc tcgcataatg cgagggccta agcccacctt gatggcacct
     5581 gctgcctcaa actgagtgat ttcagttggg gaaattcccc cttctggtcc aaagataaag
     5641 aggatttttg cccctggctt aacttctttt acttcacgcg ccaaggtcgc tagctgacct
     5701 gccttagctg tttcttcgta tgcaacgaaa atatgatcaa agccagaaag gctttttaga
     5761 aactctgctt tgtgctcaaa aaggtgaact tcaggcaccc tatttcgttt gctctgctca
     5821 gccgctccta atacaatctt agccaatttg tcttcttttt tagctaattt tttgccatcc
     5881 catttgacca ctgaccagtc tgctgggtag ccccatagcg cactggctcc caattcagtc
     5941 actttctgtg caatcgtgtc taacttatcg cctttaggaa atccagaagc aatagtcacc
     6001 ttaacaggca attccacctg atctggcaaa gcctctataa tctctaactc atgagccata
     6061 ctattggtca ccttggctaa atacttcacc ccatcatcaa agaccaagac aacttcagcc
     6121 ttgtcagcca agcgcatcac ttgaaacatg tgcttgatgg tatctttatc ggtaattgtt
     6181 acctcttttt ctgctttgcc tttaataaaa tactgttgca ttagccgcca atcactcctg
     6241 agatatcatc tgttttttta aagacaaggg cgttccactc accttgaacc atatgcgtct
     6301 caaggaagaa tcctgcgcta aaggcagctt ccagaaccat atccaatttc tcagaaatga
     6361 tgcctgataa gataaggtaa ccttcctttt tgatcaaacg gtaagcatca tcagtcaata
     6421 agactaagat atccgccaag atattggcta caatcacatc cgcttcttgg ctaacccctt
     6481 ttaaaaggtc accagcagcc acatgaatgt tgtctgttcc ttgatttaaa tcaatgtttt
     6541 cttgagccac tcgaacagct acatcatcta agtcataagc gtagatggtt ttagcaccta
     6601 gtaaggaact ggcaatcgag aggacacctg atccggtccc tacatcgata actgtctcac
     6661 caccacgaag gatttgttct agagcaaaaa ggctcatctt agttgtggga tgtgtccctg
     6721 tcccaaatgc cattccagga tctaatttaa taaccttttc cccagcagaa gcgtcataat
     6781 ccgtccaact agggacaata gttaaatcat gcgtgatgcg agctggttca tagtattttt
     6841 tccaattgtc tgcccagtct tcctcagcta attcttgact atccacagta acctgaccaa
     6901 cctgtaaccc aaaactagct aactccgcta actgttcatt aatggtcgca ataacatccg
     6961 ccaaattagt gctacttggg taataggcta tgatagctat catgtcagac tgctccacat
     7021 ctgggtataa ctccccaaag cggtcctttt gcccaatata gtctgcgcta tccgcgatag
     7081 cgaccccttg gctgcctgtc tcaatcaaca catgtgaaac agcctcttgc gcatcacgat
     7141 gcacatgaac cgtcacttct tgccacgttt ccataagtat gtcctttcta ttttgacctt
     7201 ttggttattg tcctaatggt gttacaccat ctcgtgtgtc accttagtac cttgggtaag
     7261 cgtaacttgc tgcttcttct agtccttgtt gaccttcttc aaaagcttgc gcattccatt
     7321 ccaagccgaa atcaaccttg ctgtcatctg ctaagaaatc catcaaatcg ctgtgccctt
     7381 caatagcaac atcgttgaga aattgcgcaa agtaagctaa gaattcacga gaaaagccct
     7441 ttttagcgtc aaaaggaata gtaaccaaat aatctgctgc atcaaattga gacttggcag
     7501 ggttgtaaaa gagcacatag tcctcaaaga caatatcatc ttgggtcact tcgccactgt
     7561 cgtcaatggt ttttatggcc tctttatttt gagcttctaa cacaaaagtc acttcaaccg
     7621 catgattttt cttgtcccaa tccatagcat aatcatactg aaaatgctta tccatttctt
     7681 cttctaatac tgataaaaag ccgtattttg ccatcttttt ttatacctaa tctttctaac
     7741 tgttgctgta tagagctttg taagacctca ttagtttact tactttgaca tgctgagcgt
     7801 actgttataa cttttattaa ctgaattata acatatttga ctaatattga ccataagaga
     7861 aggtcttaat ggctggatca cctctagcaa ccgcttttat gatataattt gggtatgcat
     7921 agaaaaacga ttattgattt caaagagctc gggcaacgct atctttttga tgagccatta
     7981 gtagaattgg tagccaagtc cttagatcag gtcggtccag tcattgaaaa cgtacaacac
     8041 taccaacagc tgggctatta cgtggtgggc tatctcagtt atgaagcagc tgcttttttt
     8101 gacaatgcct tacaaaccca taatgacaga ctaggcaacg agtaccttgc ctattttaca
     8161 gtgcacaaaa cctgtcaaaa aaaagaccta cctcttgatt atgacggcat tactatccct
     8221 aaccaatggg tcagcgcaac tcaaaaagaa gcctatcaaa aagccattga aaccattcac
     8281 cgtgagatgc aacagggcaa cacttatcag gttaactaca cccttcaatt aactcaggaa
     8341 ctcaatgcag ctgacagctt agccatttac aataaactcg tcgttgaaca ggctgctggc
     8401 tacaatgcct atatcgccca tgatgacttt gctgttattt cagctagtcc tgagttgttc
     8461 tttaaacaag agggcaacag gttaactact aggcctatga aaggcaccac caagcgtggg
     8521 gttaatagct ggctggatca acaagaacat gactggctcc aagctgacgg gaaaaaccgt
     8581 tctgaaaaca tgatgattgt ggatttgctc cgcaatgaca tgggaaaaat ttgccaaaca
     8641 ggcagtgttt gtgtcgatag gctctgtgag gtcgagcgat actctaccgt ctggcaaatg
     8701 acctcaacca ttgttggtga cgtcaaagcg gactgtgacc tgatagacat tctcacagct
     8761 ctctttcctt gtggctctat cacgggggct cctaaagtct ccaccatggc aattatcaca
     8821 tctctggagc caaaaccaag ggggatttat tgtggaagta ttggcatttg cctacctgat
     8881 ggtcgccgtt tcttcaatgt tcccattaga accatccagc tcagtcataa tcaagctact
     8941 tatggtgtcg gaggcggtat cacttggcaa agcaagtggg aagacgaata cgaggaagtc
     9001 catcaaaaaa cagcttttct ctatcggcac aaacaaatct ttgacctcaa aacaaccgcc
     9061 aaggtgaaac acaaaaaaat agccttcctt gagcaacacc tcaatcgcct taaagaagca
     9121 gccacttatt ttgcttaccc ttacgatgag aaagctttgc agaaacaact gtcaacctat
     9181 ttggaaaata aggataatgc tgcctaccgc ttgatgattc gtttatccaa ggatggaaag
     9241 attagcctct ctgatcaacc cttggaacct ttgtcagccg attttctgac agctcaactc
     9301 tctttacaga agaaggacgt gacagcttcg ccttttacct actttaagac cagctaccgg
     9361 cctcatattg agcaaaaatc ttatgaacag ctattctata accaagcggg gcaattattg
     9421 gaaacgtcca ttggcaatct ctttgttcag ctgggtcgga ccctctacac accaccagtt
     9481 gtggtaggta ttttaccagg actttttcgc caagagctgc tagctactgg tcaagctcag
     9541 gaaaaagagg tgacactagc tgatttaaaa gaggctagcg ctatttttgg aggaaatgct
     9601 gtccgtggcc tctatcctct caaccttaag cttactcacc ttgatgccct cttagctaag
     9661 agtcaagcct aaactgccac tcatcttttg ttattaagga acctatgata ctcttaattg
     9721 ataattacga ttcatttacc tacaacctcg cccaatattt aagtgaattt gacgagacga
     9781 ttgtcttgta taaccaagac ccaaacttat atgacatggc caaaaaagct aacgctctag
     9841 tcctctcacc tggtcctggt tggcccaagg aagccaacca aatgccaaaa ctcattcaag
     9901 acttttacca aacaaaacct atcttaggag tgtgtctggg acaccaagct atcgctgaaa
     9961 ccctaggggg aaacttacgc ttggctaaac gtgtcatgca tgggagacaa agcaccattg
    10021 aaacgcaagg ccctgctagt ctttttcgct ccctgccaca agagatcacc gtcatgcgtt
    10081 accattccat cgttgtggat cagttaccaa aaggttttag cgtaactgct agagactgtg
    10141 acgatcaaga aatcatggcc tttgaacacc acaccctgcc actttttggg ctacaatttc
    10201 acccagaaag catcggaact cctgatggca tgaccatgat cgccaacttc atcgcagcca
    10261 ttccccgtta atctcatccc ccaaaagaaa gaaggacagt tacatgcctg atcatttagc
    10321 gctcagaatg cgccccaaaa ccatttcaga ggtcatcgga caaaaacacc ttgttggcga
    10381 aggcaaaatc atccgtcgca tggtggaagc aaatagacta tcgtccatga tcctctatgg
    10441 ccctccagga atcggtaaaa cttccattgc ttctgctatt gctggtacta ctagatatgc
    10501 ctttcggacc tttaatgcca ccattgacag caaaaaacgt ctccaagaaa ttgcggaaga
    10561 agctaaattt tctggtggct tggtattatt actagacgag attcatcgcc ttgacaagac
    10621 aaaacaagat tttcttttac ctttacttga gaatggcacc attatcatga ttggcgcaac
    10681 cactgagaac cccttctttt cagtgacacc cgctatcaga agcagggtcc agatttttga
    10741 attggaacca ctttctaacg aggatattaa aacagccatt cagctagcaa tttccgacaa
    10801 agaacgggga tttccttttt tagtgacaat tgatgatgag gcgctagatt tcattgttac
    10861 cgccacaaat ggagacctac gatctgctta taattccttg gatctagcag tcatgtctac
    10921 ttctcctaat gaagatagta gccgtcatat tagtctagag acaatggaaa atagtctgca
    10981 gcgtagttat attaccatgg ataaaaacgg ggacgggcat tatgatgttt tatctgcctt
    11041 acaaaaatcc attagaggtt ctgatgtcaa tgctagcctg cactacgccg ctcgtttagt
    11101 agaagctgga gacttgccta gtttagcaag acgattgacc attattgctt acgaagatat
    11161 tggcttagcc aaccctgacg cccaagttca taccgtcacc gcactagatg ccgctcaacg
    11221 cattggtttt ccagaagctc gtattcttat cgctaacgtg gtcattgatt tggctctatc
    11281 acctaaatcc aattcagctt atttagctat ggatgcagct ttagctgatt tgagaacatc
    11341 aggtaacctc cccattccaa ggcatcttag agacgggcat tatgctggca gtaaggacct
    11401 aggcaatgcc aaagattacc tctacccaca cgcctaccct gaaaaatggg tcaaacaaca
    11461 atacctacca gataaattag taggacatca ttattttgaa gccaatgaaa ccggtaaata
    11521 cgaacgcgca cttggttcaa ataaagagcg tattgataag ctctcagact gactgctagt
    11581 tttaaaaatc gcaaccgatt tcaaaaatcg ataattttgc ttgaaaaaat tttaaaaagt
    11641 ggtattataa aagtacagaa acattgctgt ggcatacgaa tccacatcta agtgttgacc
    11701 gactatttct ttgtattttc gggaaacaaa gaccctttct agtctgcaag ccgtttcacg
    11761 cggacgcaac ttccaagaaa gagcgcgtta cccacctgct tatactgcgc gggctcaata
    11821 caaaaacgtg gatcaccggt gccaatacag cattcttgcc tccttagctc agttggtaga
    11881 gcagtagact cttaatctat gggtcacagg ttcgagccct gtagggggta tacaactata
    11941 catgaaaaaa cctttgttta aaggcttttt ttgcttgtca gaagaggatt tgttccatca
    12001 tttatccccg cagtaatttt tttatggtat gataccaatt aaagcgctta agattaaggt
    12061 ggagactgtt atgaaaataa ttgagtacaa aggaaattac agagatgacc tcatttttat
    12121 ggtcttggaa gcaaaaaatg ccttaaggag aattcccagc attaacccgg atcttttgac
    12181 aattgaaaca agttatttgg aaaaagggga ttgtttttgg ctagcggtca atgaagaaag
    12241 tcgtgttatt ggctcaatcg cttattcttc tattcccaaa acaaatgagg tcttcttgca
    12301 ccgtctcttc atcaaaccaa gcttaaaaca tcaaggcatt ggcagtcaac ttctgacgtt
    12361 tgcagaagat tatctaagga aaatcggcaa gagtcatgtt cgtatccact taggtcaacc
    12421 taaagaacaa tggtttgagt cttatcggtt ttatcctaaa catggttatc aattttatga
    12481 tgacacgcat ctcatcaaga aactaatcta aacacatttc atacccttta tagtcatttc
    12541 atacgtcatt tttaggaggt atccattgaa aattgccatc attggacata gtggctctgg
    12601 aaagtcaacc ttagcacgat ttttaggtca acattaccat tgtgaggtgg tccatttaga
    12661 ccaactacac ttctcatcga actggcaaga acggtcagat catgacatga tagctgattt
    12721 atcaacatgc ctgctcaaac aagattggat tattgagggg aattatgcca attgtctcta
    12781 tgaagagcgc atgagtgagg ctaatcacat tatctatgtt aatttcagtc ggtttcattg
    12841 tgtctatcga gcatttaagc gttatttaaa ctatagggga aaaacgcgtc ctgatatggc
    12901 tgacaactgt caggaaaaat ttgacgttgc gtttgtgaaa tggattctac tagatggacg
    12961 ttcaagaaac caattaaaaa aataccaatc cgttatccaa aaataccctc ataaaaccat
    13021 tgtcttaaca aatcaaaagc agctcaatca ctatatgaat acaatcaact aattagagtg
    13081 aaaaactaaa atagccacgg gtaacccggg ctattcagac tgaaagcctt tgtttaaagg
    13141 cttttttgct tgtttcataa ctaatcggct taattaaaag cgtcaatcca cttttagtat
    13201 catgtctcaa ttttcatttg atagtcagca agtatatcta caaaattgt
//
LOCUS       DATUYR010000043        12787 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.043, whole genome shotgun sequence.
ACCESSION   DATUYR010000043 DATUYR010000000
VERSION     DATUYR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.043"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(147..887)
                     /locus_tag="VCI33_001222"
     CDS             complement(147..887)
                     /locus_tag="VCI33_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEP2832147.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTILFSYIASFVWNPDKHRH"
     gene            927..1781
                     /locus_tag="VCI33_001223"
     CDS             927..1781
                     /locus_tag="VCI33_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEP2832148.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            1771..2049
                     /locus_tag="VCI33_001224"
     CDS             1771..2049
                     /locus_tag="VCI33_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEP2832149.1"
                     /translation="MTTKKAYMYVLECADKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(2073..4073)
                     /locus_tag="VCI33_001225"
     CDS             complement(2073..4073)
                     /locus_tag="VCI33_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEP2832150.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKISPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLISEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLAMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVTNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(4200..5819)
                     /locus_tag="VCI33_001226"
     CDS             complement(4200..5819)
                     /locus_tag="VCI33_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2832151.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            complement(6126..7670)
                     /locus_tag="VCI33_001227"
     CDS             complement(6126..7670)
                     /locus_tag="VCI33_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEP2832152.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNVFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            complement(7918..8613)
                     /locus_tag="VCI33_001228"
     CDS             complement(7918..8613)
                     /locus_tag="VCI33_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEP2832153.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            complement(8693..10084)
                     /locus_tag="VCI33_001229"
     CDS             complement(8693..10084)
                     /locus_tag="VCI33_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEP2832154.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVD
                     RLVTDKNCDSYQP"
     gene            complement(10283..11329)
                     /locus_tag="VCI33_001230"
     CDS             complement(10283..11329)
                     /locus_tag="VCI33_001230"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEP2832155.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            complement(11430..12026)
                     /gene="recR"
                     /locus_tag="VCI33_001231"
     CDS             complement(11430..12026)
                     /gene="recR"
                     /locus_tag="VCI33_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEP2832156.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
BASE COUNT         3982 a         2673 c         2268 g         3864 t
ORIGIN      
        1 gtcatttttt gtgcttaaaa gagttaaggg tatcatcgaa tatctttttt agaaatgaaa
       61 aaagtgatta agcattagcg gcttctttca atagaagact aatctcaatc acttcttatt
      121 tcaggcacat gttagccctc atgttcttaa tggcgatgtt tatctggatt ccaaacaaaa
      181 ctagcaatat aggaaaataa tatggttaat aataatacca ccaccaaaac caatcctaat
      241 ggtaaacggt agagataagt taaagggttc cttgcttttc ctggttggaa aggtgccaac
      301 tcgtcatcca tccgatcaaa ctctgcctga atacggtttg ccacctcagc aatcccttca
      361 tcattcatgc gtttaatatc tgagacgtca atgggattcc caaaagtcat gtcaacccgt
      421 tctcccgcta gtaatccctt gaccgacata ggcccctgat aggctgctgg cataatcttg
      481 accttagcca atttagcaat cacagcgacc cctcctttga cctcttgcga atgcctactt
      541 ccacttggaa acataagaag agaacgattg ctctttttga gcatattaac agggtaacga
      601 atagcatctg gactcggttt gtcacgatca ataggaaaag caccacacat tttaatccac
      661 caagcaaaga gacgatttgc aaacaactct tttttagcca taaaaataaa ctgcttagga
      721 cgtgctgcaa atgccatgta aactggatcc caaaaggttc gatggggtgc taccagaata
      781 taattctcag aagcatctaa catcttttct tcatgatggt agtgggcatt gccattgaca
      841 acccataata aaaagactac taaaccgcgt aagtaagcgt aaaacacagg cttctccttt
      901 aatagaataa tcttacttat tgtatcatga aagctgactt ttcgaaagtc aaaatcactc
      961 ttcaaacatt cacctcaagc ctattttatg ttaaaataac aggtatgata aaagcaatct
     1021 taaaagaagg cgaacgtatt gatcaactct tttcaagtga tgttggcatt attcaaaaca
     1081 aggatgtctt tagttactct attgatagcg tcctcttatc ccgcttcccc aagatgcctt
     1141 ccaaagggtt aattgtggat ttgtgctcag gaaatggggc ggttggctta tttgctagca
     1201 ctagaaccaa ggcagccatc gttgaagtag aattacaaga acggcttgct gatatgggac
     1261 aacgctctat ccagctcaat caactggaag accaagtaac catgatttgt gatgatttaa
     1321 aaaacctcct aaaccatgtg ccacgttcag gagttgattt gatgctctgc aatccccctt
     1381 actttaaaag tcacgaaagt tctaagaaga acgtctctga acattattta ctggctcgtc
     1441 atgaagtgac cactaattta gaagagattt gtcaggtagc ccgtcatgcc cttaaatcaa
     1501 atggtcgtct ggcaatggtt catcgaccag atcgattctt ggaaattata gatagcctta
     1561 gagctaatgg tcttgcccct aaaagggtac aatttgtcta tcctaaatta ggtaaatcag
     1621 ctaatatgtt acttattgaa gcaatcaaag acggttctat tgaagggatg actattttac
     1681 ctcctttagt tgtccacaag gaaaacggag aatacactga tcatattttt gagatttatt
     1741 ttggtgctgc ttccaaagga aaacctaatc atgaccacta aaaaagctta tatgtatgtg
     1801 ttagaatgtg cagataaaac cctctacact ggttatacaa ctgatttgaa aaagcgttta
     1861 gctacacata acgcaggaaa gggggctaaa tatactcgct accggttacc tgtttcttta
     1921 ctttattacg aagtgtttga tagcaaagag gctgcgatga gcgctgaagc actctttaaa
     1981 aaacgaaaaa caagaagcca gaaattggcc tatattgcta ctcatcaaaa agaaaagaaa
     2041 aatcattgag acttatcatc tcaatgattt ttttatgctt cactgtcttc cacaggcacc
     2101 aattctttga ttggtaactt caagacatct gatagaatca agggaaccac ttcaagggtt
     2161 acttcggaat attgaagccc aaaccaacgc attggactag cggtaacatg tttaatgctt
     2221 gccttagtct gcataataaa gcgttcaaag ttactcaatt gacgtgcatt ggtgacccgt
     2281 tcttctatta aaacaaagcg aaaatcacca acatcacgac caggtgtgat ggtatattcc
     2341 tgttcttgtt tgggtaaacg gccactttcc atcaaatcct gaacaatcgt gcgcaaatag
     2401 cgtggcacag tctgtggcat tctaaaacca agataaaggt tgactcgaac catataatcc
     2461 gttcccatca tatctacctt gtacttagct gtataaggtt catcagttac ctggacattt
     2521 acaaaccaat aaacttgagc gcgttttggc cgcttgtcta aaatggagta caaaatagac
     2581 cggtcaatca tatgatcctg catacgattg gataagtaga caacatttgt ttggtagagg
     2641 tcaaaacaaa cgtcgtcacg aagttgttta atctgctcct tgtaatcatt caaattaaga
     2701 gatttcacat acttgaaaac aatccgtgtg cccgcatgcc agataaacat gacaaagaca
     2761 attgcaagtg ccaagataac aaccgcatag ccaccgtgca taaatttgat agctgaagcc
     2821 aagaagaaaa tgaattctac aagggcaaag aaagccatcg ctaaatgagc caagatgggt
     2881 ctcgttcctt tcttgattaa gtaatacttt aagaggattg ttgtcatcaa catggtgatg
     2941 gtaatagcaa gaccataagc tgcctccatg tgggctgatg ttctaaaagc caaaacagta
     3001 caagaggtca ctgcaaataa aatccagtta atgacaggaa tatacaattg ccccaaattt
     3061 gcccctgggt aagtcacacg aaacaaaggg aaaattttca aacgcatggc ctctgaaatc
     3121 aaggtaaagg agcctgaaat aagagcctgt gaggcaataa tagctgccaa ggttgccaaa
     3181 ctaacaaggt aaactcttaa ttgtgatggc acactagcaa agaaagggtt tagttcaata
     3241 cctgagtgtt tattcgctaa aatccacgca gcctggccac agtaagacaa cacaatacac
     3301 atcttaacaa agggccagct cacataaata tttcctcgac caacatgtcc taaatccgaa
     3361 taaagggctt cagctcctgt tgtcgcaaga aaaatggacc ccaagataaa aatcccacga
     3421 tggttttcag gactaaacag caaatgcaga gcatagtaag gattgatagc cttgaaaatt
     3481 tccagatgcc ccaacgtatt aaaaaaacca ctaacaccta gaaaactaaa ccagataaac
     3541 ataactggtc caaaaatttt accaataaaa cctgttccaa accgctgaat accaaacaag
     3601 actattaaaa tcactagagt tgtgataatg acattggttt gattttggta gatgtgactc
     3661 agaccaggaa cggctttcaa gccctcaata gctgaagtaa ctgttaccgc aggtgtaaga
     3721 gccccatcag acaaaagtgt tgcacctcca atcatcgccg gaataatgag ccacggtgat
     3781 attttacgta ccaaggtgaa taaagaaaaa atcccacctt catggtggtt atcagctttt
     3841 agagcaatca aaacgtactt gattgttgtg atcaaggtca gtgtccaaat aattaaagag
     3901 attgatccta aaataaagga ttcagaaacc tgattaaccc ctccttgatt ttcaactaga
     3961 gactgcatcg tataaagggg gcttgttcca atatctccat aaacaatacc taaagcaata
     4021 ataaaacctg cttttgaagc tttgtcaaag gcagtaagat ggctatcaga catcttatac
     4081 tcctcctaat tttttaaaaa ttataatgaa ataactgaaa ccattataac agatcccatt
     4141 ataaaagcaa ctttttcaga aaattaaaaa gagctgactc tcatcaactc tctttaagct
     4201 tattcacctt tgtgacgaat cacaaaacca gtatttttag cacttgattt tttctcttta
     4261 ttgaaaaagt ctttactatt acgttttgat ttacgtttga aatcgcggtt gccactgcca
     4321 tcgtgaccat cacgcttatc acgtttttga cgacgacggt caccatcacg ctcatctctg
     4381 ttacggtcac cacgatagcc accacggcga tcaccacggt tacggttgtc acgtccacga
     4441 ccacctttgc catttttatt gccgtgacca ccacccacat atttgaatgg taatggtttt
     4501 tcacgtgcaa tttcaacttc cggtaagcta tctggatctt gaaccgttaa gctcaaaata
     4561 tataaagcta attcttcagg agtgaattcg gcagccaatt ggacagcatc gcctttgaat
     4621 ttatcaaagt ttgaacgaat agtctcatcc gcaaagtcgc gttcaatttt cttgagggct
     4681 acttttttct tggcttgaaa ggcctcttca gcagttgctg gacgaagagg tttcatctgt
     4741 tttttcgtta agttctcaat cattgagagg tagcccatct catttggtga gacaaaagtg
     4801 atagactcac cagatttacc ggcacgacct gtacgaccaa tacggtgaac ataactttct
     4861 ggatcttggg taatatcgta gttataaacg tgggtaactc ctgaaatatc aaggccacgc
     4921 gctgctacat cagtggcaac aagaatatca atttggtcgt ttttgaaatc acggataaca
     4981 cgtaagcgtt tattttggtc aagatcacca tggattcctt cggcacggaa accacgaagt
     5041 ttcaagccac gtgtaatttc atcaactcgg cgcttagtcc gcccaaaaac gatggataat
     5101 tctggctggt taacatccat cagacgggtc atagtatcaa atttttcttg ttctttcaca
     5161 cgaacatagt attgatcaac attgacgttt gttaattctt tgttcttaat ttgaacatgt
     5221 tcagggtctt tcatgaattt aaccccgatt tgtttaatag gtgccggcat cgttgcggag
     5281 aagagcaagg tttgacggtc tgctgggaca cgactaataa tagcttcaat gtcttccaag
     5341 aatcccatgt taagcatttc atctgcttca tcaaggatta aggtttccac atgatctaaa
     5401 ataagagcct tgcgcttaat caaatctaac aagcgacccg gtgttcccac gacaatgtgg
     5461 gcacctgatt taagggcttt gatttgtttt tcaatacttg atccaccgta aacagagcga
     5521 accttaaccc ctttttcacg tccaaaacgg aacaattctt cctgactttg tacagccaat
     5581 tcacgcgttg gtgcaatgac caatgcttgg atgatatttt cgttagtacg aatcttattc
     5641 aaggttggta gaccaaacgc tgctgtttta ccagttcctg tttgtgcttg accaataaca
     5701 tccttccctt ctagtgcgag aggaatcgtc atttcctgga taggagatgc tttctcaaaa
     5761 ccagcggtaa caacagctga ttggatgtct tgtgataagt taaattctgt aaatttcaat
     5821 tgtttctctt ttctaaaaag gcagtgcgaa gctgccttat aaacctttta attttcgtcg
     5881 tttgattaac aacctaacta gtgtaacaca attagaatca aaaagatagg aaataaggct
     5941 aatataagca ataacacgct ttcacgatta tccgctaaat cataaatatg cttacagttg
     6001 tgaagatatc gttttttgat agaatcttaa atctgcaaaa gagataacta gccactcatt
     6061 tcttttaaaa agttgctcag taaattgatc tccaaaacgt tagactgttg aaaatcatgt
     6121 ttcgattaca ttttctcttc taaagtgaca tctgggtatt tgtctgcaaa ccagcgaagg
     6181 gcaaaatcat tttcaaatag gaaaactggt tggtcaaaac gatccttagc taaaatgttt
     6241 cggcttgagg acatgcgttg atccaaatca tcttctgaaa tccagcgaac agtcttttta
     6301 cccattggtg tcatcacaac ttcagcgttg tattcacctt ccatacgatg tttaaagact
     6361 tcaaactgca gttgacccac agcacccagc atgtattcgc ctgtctgata attcttataa
     6421 agctgaattg ctccttcttg tactaattgt tcaatacctt tgtggaagga tttctgcttc
     6481 ataacatttt tcggagaaac cttcatgaag atttctgggg taaaagttgg taagggttca
     6541 aattcaaatt tatttttccc aactgttaag gtatccccaa cttgataagt tcctgtatca
     6601 taaaccccga taatatcccc agcaacagca ttcgtcacat tttcccgtga ttctgccata
     6661 aattgagtaa cattggagag cttagcgcct ttgccagtgc gtgtcaaatt aactcccata
     6721 ccgcgctcaa attcaccaga aacaatacgc acaaaagcaa tacggtcacg gtgtttaggg
     6781 tccatgttag cttgaatttt aaagacaaaa cctgaaaaat ctttagctaa ggggtcaaca
     6841 acattacctt ctgtagtctt gtgaccatga ggttctgggg caaactccaa gaaagtatca
     6901 agaaaagtct gaactccaaa gttcgtaagg gcagagccaa aaaagactgg agttaggtca
     6961 ccatcaagaa tagcttgttc tgaaaagaca ttacctgctt cttgaagaag ttcaatatct
     7021 tctttgactt gttcataaaa gggattattg gcaaacagct gatcaccgtc ttcaatactt
     7081 gcaaaacgct cgtctccctt atagagttct aaacgtttat tatgtaaatc atataagccc
     7141 tcaaaggcgc gtcccatacc aatcggccag ttcatcggat aagaagcaat ccctaaaact
     7201 tcttctaatt cttccaagag ttctaaaggc tcacgcccat cacggtctaa cttgttaata
     7261 aaggtgaaga caggaatatt acgatgtttg acaacttcaa aaagtttttt cgtttgcgcc
     7321 tcaatacctt tagccgaatc tacaaccatg acagccgcat ccacagccat taaggtacgg
     7381 tatgtatctt ccgagaaatc ctcatgtcca ggtgtatcca aaatattaac acgtttaccc
     7441 gcgtaatcaa attgcataac agatgaagta acagagatac cacgttgctt ttcaatatcc
     7501 atccagtcgg actttgcaaa cgtacctgat tttttccctt ttactgtccc tgcttctcta
     7561 atttcaccac caaaatataa tagttgctca gtaatagtcg tcttaccagc atccgggtga
     7621 gagatgatgg caaaagtacg gcgtttttta atttcttctg taagtgacat tcttttttcc
     7681 ttgtatagta aaatggcaga gcttttctct gagttatttt cgttaggtat tacttgttta
     7741 tcaaaagagt tgggctacat tgggtttctc catatacgat tagcctctaa agttcttcaa
     7801 aactggtatt ataacttatc ttgggctatc aatgagtatc ttttttaaaa aaatcgagcc
     7861 tggaaatcat cccagactct tcttattata gctgatttta agtgcttttt caacgaccta
     7921 cttttcttta gagaaagcaa atgccagctg ttgagcttca ccaaatttaa ggtaaaaata
     7981 ttcggttatt ttaaccataa aagtcaagcc acttgtaacg atcacaaaat taagtagcaa
     8041 atgacgcgta tgaatcactg gaatatccat cacaaaacca taatttcctt tcgtggctaa
     8101 actgaccaac agaagaagaa agttaacagt tgctaaatag cgtactactg atagcagtct
     8161 taactgactg gcatcgtaaa aacgcaagag gtaaatcaaa ccattcacta acaaagcata
     8221 atgaccaaga taaaacgaga catttgctac atgccataat ctaaacggat aaagatctgg
     8281 cgataaaagt gcaaggaatg ttccacctat ccccaaaacc ataaataatt gcttaaactt
     8341 gttacgatca ggtagaaaaa agatagctaa catagcgatg cgacaatgat acaacggcaa
     8401 agcttcatct aaaggaaacc cccttaacac gtaccatgtg tacaagccaa tcacctgaga
     8461 taactgtaaa aacaagaaaa aatatcgata agattttaac ctatatgctt gaaaagtcaa
     8521 aaaaacaagc aaaagcgcta tcaagagtga cgacaagtaa aagataagtg aggtatgagg
     8581 cagtccgatg gggtcaattg caaaaaaatc cataatcgtc cctttctata ttttttcatt
     8641 ataccttaat cttcatgaga gaacaagact ttatctgaaa gttttataaa aatcaaggct
     8701 gatagctatc acaattcttg tcagtcacta atcgatccac taacttttct agcgacatcg
     8761 agtggctgcc ttttaaaaga atttgatcaa agggattaag aatgttttga acatattgtg
     8821 ccatggcttc aaactggtct tcttgttcag tttttaaaaa ataatgtact tgctcagcag
     8881 gataaacctg acttgccaac cgtgctaatt ctttaatatg atccccataa aaaacaagct
     8941 gatcaatatt acctgatgtt aagctgtcaa ttaactgaga atgaagtatc actgaatcct
     9001 tcccaagttc tttcatatct gctagcactg caatcttttt tcctccgggg ttctttgcta
     9061 tgttagcaaa tgtttctaaa atcagacgca tggctgttgg gttggcattg tagacatctg
     9121 ataaaatatc agcaccattg gctgcttttt tccactccgt acgattacct gtcaacgtca
     9181 cagtctgtaa agcagctata atatcctcgt ctgttactgc caatagtttt cctacatagg
     9241 ctgctaccat agcattggtc gcgttgtatt ttcctggtag aggtagggac acaggtgttg
     9301 ctaacacatt tgttgtaaac gttaagctat ctttgtcttc ttgaatacca gtaacgtcaa
     9361 tttcctgctg atttccaaaa cggataacca tctgattttc tggaagatat gggtcaataa
     9421 tcggatctcc cggagcaatt aaaatcccat ctgaattcat gccgtcaaca atctgcattt
     9481 tgccttgggc tattttgtct cggcttccaa aatattctaa atgagcttcg ccgactaagg
     9541 ttaaaactgc aatacgaggc cttgcaattt cagacagaag acgaatatct cccatgtgat
     9601 cttgtcccat ttcaagaaca attttttccg tatcgtcagg catgtggaga acggtgtaag
     9661 gcaaaccaat ctcattattg taatttcctt gcgttttgta agttttgtaa gtggttgata
     9721 ggacagctcc tatcatatct ttggtggaag ttttaccatt tgagcctgtc acagcaataa
     9781 catccacgcg catcttatca atataataat gggctagctt ttgaaaagct tttaggcagt
     9841 cttcaacaag aaggtgaggt ttccccggca attctttttc tgaaaaagta gcgacagctc
     9901 cattttgaaa ggctaaatca ataaactcat gaccatccct ttgacctttt aagggtaaaa
     9961 agaggtctcc ttttgtaatc tttcggctat caaattcaat gtgatgcaga ggaacgtcgt
    10021 ctaagtcaga cacattattt tgggcgtcaa ctattttagc cacttcgtgt aaggttaatt
    10081 tcattttgtt ttctttctaa gaacactagt atttacgatg tcctatctat tatagcacaa
    10141 aacattagaa aaatttctaa aatgtcaaac tcccttaatg gctctactat tcttcctaaa
    10201 agagcaacaa aaaaaggacg aacgtcacat cttgacgatg tttgccgttc catccaagtc
    10261 aatgtttatt catctcatag gtttaaatca ggtggctctc acgctggtca aacatttctt
    10321 gtgccagtgt caccagctct tcaatcaaat ctggataagc caatcccata ttttcccata
    10381 agagtggata catagaccat tgggtaaagc ctggcatggt gtttaattcg ttaaggtaaa
    10441 cctgtccgtc ttgggtcaag aaaaagtcac aacgggacaa cccacaacca cctaaggctt
    10501 taaaggctac ttcagcataa gaacgcattt ctgtcacaat actctggtct acatctgctg
    10561 gaatggccat cgtaatctta ttgtcaacat attttgcttg gtagtcgtaa aaatcaacgt
    10621 ccttgattac ttctcctggt agagttgatt taaccttatc atttccaagg agaccaactt
    10681 caatttctct ggcaaccacg ccttgttcaa tcaagacacg actgtcatag gtcagggcta
    10741 actgaatcgc ttttcttaat tctacttttg tttgggcttt ggaaatcccc acagaagaac
    10801 ccatattagc tggttttacg aagataggaa aagtaagtct tgctaaagtt tcaactaaac
    10861 aagcctccaa atcttgtccg tcaatataaa cagtataggc aacttgtgga attccaattg
    10921 attctaagac acgtttagta gtaattttat ccatagctat gctagaagac atgacattag
    10981 tccctatata tggcattcta agcacttcta agaaaccttg gatagagcca tcttctccca
    11041 taggtccatg taatacagga aatacaactg caccttcttc atagatatca cttggtttta
    11101 ttttttgagt tagctctatc gtctcatttg tcatcaagcg ttctgattcc gatggctttt
    11161 ctgaaaattg ctgagttttg ataaattggc ccatctgagt aataaaataa gtttttacta
    11221 agaacttatc atagttgact gctcgcatca cactctctgc tgaaagcact gagacttcac
    11281 gttctgctga gcggcctccg tataatagaa ctaacgtttg tttggacata gctaaccttt
    11341 cttatctgaa ttgaatgttt ttttattata acaaaaagat gtagcctttt caaaacctct
    11401 ctttgcgagt aaaggcacca acatgatcat taaagttcag tacgattctc aatagctctc
    11461 aataatgtta cttcatcagc atactcaata tctgaaccta ctgcgagacc tcttgccaaa
    11521 cgagttacct taatcccggc tggtttcaag acacgtgaaa tatacatgga cgttgcttcc
    11581 ccatctgctg tggcattggt agctacgata acttcgctca ccttaccatc cattagacgg
    11641 gtaattaaac ttttaaggtt gatgtcatct ggccccacac cattcatagg cgaaatcaag
    11701 ccgtgaagca catgataata gccatgatac tcttggattt tttccatggc agaaacatct
    11761 ttagcatctt ctactaccag aatggtcgtc tgatcacggc tcgtgtctgt gcaaatgtga
    11821 caaggatcgt catcggtaag gtttccacaa atcgaacaat aggtcagttc tcttttagct
    11881 gctaataagt ttttagcaaa atcattgaca tcttcatttg acattccaat agtataaaag
    11941 gctaatctcg tcgccgtctt gataccaatt cctggaagtt tagagtaact gtcaattaac
    12001 tttgcaatgg gtgttggata aagcactaag ttctccttta ttgttttcca tatatcgcca
    12061 cataaagcgc cctcacatct ttgccgatat attcgtaata tgtggataca taacagcaac
    12121 cgcaatatta tcttcgttat ttgtattata agctactaca tttaagttgt aagttactaa
    12181 ggtctgatct gaattgtctg tgatacaagt ctcagcagtt ccaattttag cgtgaatcgt
    12241 gacagaaccg tttggattag ccaagtaaaa acttttttac tcgacttgtt accttggcct
    12301 tgagatattt ggcttttgtt ttttgattca ttttactttt ttagaaaccg caaaccatcc
    12361 ttatgatata agtttatagg aattgtttca ttccatgcag aaatgatgtt tactcaattg
    12421 actatgctat ttttctcaac aagagtgcat aaagcgaact catggttatc tttaagcttt
    12481 catgctacac tttcttcact aaaagtgtat taccaaaagc attattaaga aatctctaag
    12541 cacaaaccat aagacagttt gtacgcctga tgaatctgtt gctgcatcac tgtccacttt
    12601 ttaccctatc aaggctattt ttgaaagaca actaatgatc tgcattattt tgggaactac
    12661 tcccatagca aattcttttt acggattttt tgggaatact ccttttttag ggaataaact
    12721 aaccattatt tagctaaatt ttttaaactt cgcttcttat tttaccataa attataaaaa
    12781 gaagact
//
LOCUS       DATUYR010000044        12583 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.044, whole genome shotgun sequence.
ACCESSION   DATUYR010000044 DATUYR010000000
VERSION     DATUYR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12583
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.044"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(54..623)
                     /locus_tag="VCI33_001232"
     CDS             complement(54..623)
                     /locus_tag="VCI33_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2832157.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(681..1226)
                     /gene="coaC"
                     /locus_tag="VCI33_001233"
     CDS             complement(681..1226)
                     /gene="coaC"
                     /locus_tag="VCI33_001233"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEP2832158.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(1219..1911)
                     /gene="coaB"
                     /locus_tag="VCI33_001234"
     CDS             complement(1219..1911)
                     /gene="coaB"
                     /locus_tag="VCI33_001234"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEP2832159.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNKLLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            2092..2943
                     /locus_tag="VCI33_001235"
     CDS             2092..2943
                     /locus_tag="VCI33_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEP2832160.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            2940..4139
                     /locus_tag="VCI33_001236"
     CDS             2940..4139
                     /locus_tag="VCI33_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEP2832161.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVINQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNIAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            4136..5134
                     /locus_tag="VCI33_001237"
     CDS             4136..5134
                     /locus_tag="VCI33_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEP2832162.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            5176..5508
                     /locus_tag="VCI33_001238"
     CDS             5176..5508
                     /locus_tag="VCI33_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEP2832163.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            5501..6313
                     /locus_tag="VCI33_001239"
     CDS             5501..6313
                     /locus_tag="VCI33_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEP2832164.1"
                     /translation="MPSSFDLLGEMIDLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGRHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            6306..7187
                     /locus_tag="VCI33_001240"
     CDS             6306..7187
                     /locus_tag="VCI33_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEP2832165.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            7234..8253
                     /locus_tag="VCI33_001241"
     CDS             7234..8253
                     /locus_tag="VCI33_001241"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP2832166.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            8381..10051
                     /locus_tag="VCI33_001242"
     CDS             8381..10051
                     /locus_tag="VCI33_001242"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP2832167.1"
                     /translation="MKSDIEIAQSVALQPIIDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLLLVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFTEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            10136..11713
                     /gene="cls"
                     /locus_tag="VCI33_001243"
     CDS             10136..11713
                     /gene="cls"
                     /locus_tag="VCI33_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEP2832168.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLTQRP
                     WYQKLIGLLVRTIAPLL"
     gene            12053..12391
                     /locus_tag="VCI33_001244"
     CDS             12053..12391
                     /locus_tag="VCI33_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832169.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT         3903 a         2165 c         2622 g         3893 t
ORIGIN      
        1 gttaaaataa ccaagttttt aaaagagaaa aactcggtta ttgtgaaagc taattacctt
       61 tttagtcttg atagtgttgg aataagtaca aaggtaataa tagctgagat gatcatctca
      121 acaatagcgt ttgatgagat gatggctgtt agtagtgctt taatatttcc atcaaaaact
      181 gtcgcgaaaa aaataaatat cccagataat acaaatatag tatttgttag agaaccaatg
      241 ataccggata ctattaaacc aaactgatta ggaatcaatt tatagcaata ataaggggta
      301 ataccaatca aaatacgtgg gataatagca attattaaag aagcgaaagt tccatgatcg
      361 acaaaaggtg aaaagagata atttgtaggc agtagaatga tggtatttgt aatgacactt
      421 ataatcccca ttaatcctcc taaaatagca cctatccttg ggccatataa gacagaagca
      481 ataataactg ggatatggac aagcgttggt ttgattggaa taggccaaat attaaagaca
      541 agactgctca caaaatgaat gactaacatg atggcaaaaa agatagcaat acgagaaata
      601 tctgctgatt tacggtgatt cattttactc tccaaaaaat ataatactta attctaaaaa
      661 ggtgatcaga aaaacgactg ttaaagtttc caaatcgtat caatagtagc taagataaca
      721 tcaatatcag ccaaagcacc aggtccttta tctccacagg ctaataagct agattttgga
      781 ggaatttctg taaaccctat tgtcgataag cgtttaatat tttcttgggt aatagggttt
      841 tgatacattt tggtattcat ggctggggct attaattttg gtgtggtagc tggtagggca
      901 agagcaacac ttgttacaat attatcagcg aatccatatg ctagatgagc aatagtatta
      961 gcagaggcag gtgcaacaat aaataaatct gtgcgttttg ctaattcgat atggttgata
     1021 acttttgggt catgctcatc cataacatct aaatgaatag cattttttga taacacttgc
     1081 agggttaatg gtgtaataaa ttgtgtagca gcctgagtca ttatgatgtg aacatcatag
     1141 ccaattttag ttaattgact cgtaagatct gctgctttat aggcagatat actaccagaa
     1201 acagctaagg taatatgttt agtcatgttt ggtcatcctt tcatataata agtcagcaat
     1261 tgcctctttg gtatctgcag atgcaacttc gttattggat atcaaaagag ccttgtgcat
     1321 acctgtttgg atatcaacta aatcattggc gagaatataa tcagcatggt ttttagctag
     1381 acttgctctg gctactttaa ttaactcttc ttgaggcaca ttaactagaa gtttaaagcc
     1441 gactagttta atattaggat tccactgttt aacataagag ataactttgg gtgttttttt
     1501 caaaaaaaga acctgataat cagatgcaga tgagattttc ggctcactgt tatgttcaca
     1561 tagaaaacaa ttaaggttat cagcttgaga gacttgttcc aaatcagtca tgtagactgg
     1621 agtatagtca gaaacagcca tactatgaat tagaatgtca tggtgaggaa cctgatcttt
     1681 gagggcagcc attaagtcgt taactgtttc cacttcaata atccttagga gcttatttgg
     1741 caggggttta gtggctgttt tagtagtgac tagggtaacg tcatgatgat attgtaaaaa
     1801 tcgctctgtg attaattttc caagttgccc agttgagtga ttagtgatac ctcgtactgc
     1861 atctataggt tcagttgttc cacctgatgt tattattaat ttcatagtca tattttatca
     1921 aaaaaggagc tgatagtaaa cggaagataa tgacgccaga cattgtcttg cgtttgtatt
     1981 aatatggaat agtaatagat ttctagtaat taaaacgaag ttctggaaag caagacacta
     2041 tagtagctaa aatctaaatt ttaaggtaaa atagagcaag cggtttcaag tgtgaaagga
     2101 agaattgtga taactattag agatttagcg tcactgccga tttttgttta cggtgatgga
     2161 aataaaaaag ttccaggagc tttgagtcct tttgtgtgga cagaggtgtt tttaaaaact
     2221 atcaatcaag aacctaatca actgatctta catatttggc cgatgacaag aacagttata
     2281 ttagggatgt tagatagaca gcttccctac tttgagctgg ctaaaacaga gattggcaat
     2341 aatggttatg ttcctgttac tcgtaatatt ggcggtttgg ctgttgtcgc tgatgatggg
     2401 attttgaatt tttcgcttgt tattccagac catttttcgg aatctatcag tattagtaat
     2461 gcttatttaa taatggttga tgtcattaga gagagttttt cggattatta tcagcgtatt
     2521 gaatatcacg aaattaaaaa ttcttattgc cctggtaatt ttgatcttag tattgcaggc
     2581 cgcaagtttg caggtatcgc ccagcgtcgt atcaaaaagg gaattgttgt gtctatttac
     2641 ttgagcgttt gtggggatca agcagcgcgt ggtcaactca tcaaggattt ttatgaggcc
     2701 ggaacacaag gagaagttac caaggttaat taccctcaaa ttgatccaga atgcatggca
     2761 actctttcag aactccttga gacgcctttt acagtggcag aagtccttga acgtcttcga
     2821 ttgaccttgc gacagcttgg cttttccctc acggaaaaaa gtccagatca agccttactg
     2881 actaattttg atgctgtcta tgaacggatg cagctagaag tcgtcagaaa ggaaggcaaa
     2941 tgaaagaaag atttagtcct ttatttgagc ctttgacttt gccaaatggg agtcaattgg
     3001 ataatcgctt tgttttgtct cctatggtga ccaactcatc cacaaaagac ggttatgtca
     3061 cccaagatga tgtatcctat gctttgagac gagcagcttc tgcccctcta caaattacag
     3121 gagcggccta tgtggatcct tatggtcaat tgtttgaata tggttttagt gttacaaaag
     3181 atgccgatat ttctggcttg aaagagctag ctcaagcgat gaaggcaaag ggagctaaag
     3241 ctgtcttaca actgacccac gctggccggt ttgctagcca tgccttgact aaatatggct
     3301 ttgtctatgg gcctagttac atgcagttga gatctcccca acctcatgaa gtcaaacctt
     3361 taacaggtca gcaaatagag gaattgattg cagcttacgc tcaggccact cggcgggcaa
     3421 ttcaagcagg ctttgatggc gtggaagtat cttcggcgca gcgcttattg attcaaacgt
     3481 tcttttcgac tttttcgaac aagaggacag atagttatgg ttgtcaaaca ctttttaatc
     3541 gctctaagtt aactttagct gttttgcaag ctgttcaaca ggtgattaat caagaagctc
     3601 ctgatgggtt tatttttgga tttcgtgcta caccagaaga aactagggga aatgatattg
     3661 gttatagtat tgacgagttt ttgcagttga tggattgggt acttaatatt gcaaaacttg
     3721 attatttagc gatagcaagt tggggccgtc atgtatttcg aaatactgtt cgttctcctg
     3781 gcccttatta tggaaggcga gttaatcagg tagttagaga ttatttaaga aataagctac
     3841 ctgttatggc tacaggggga atgaacacac ctgacaaagc tattgaagca ctagctcatg
     3901 ctgattttat tggagtatct acaccttttg tcgtggatcc agaatttgct cataaaatca
     3961 aagaagggtg tgaagagagt attcatttaa ggattcgacc tgctgatttg aaaagtttag
     4021 cgattcctca ggcttctttc aaagatattg tacctttgat ggactacggg gaatcattgc
     4081 ctaaagaatc aagaacttta tttcgtagtc ttactcacaa ttataaggag attaaatgaa
     4141 agtcagtatt cttgattatg gggtgattga taaagaaaaa acaccccaag aagctttatt
     4201 agaaacgcgc tgcttagcac aagtagccga caaacttggt tttcatcgtt tttgggtagc
     4261 agagcatcat aatatttatg cctttgcaat aagcagtcca gagctgttga tgatgcattt
     4321 agcagatcat accaaacaga ttcggatagg ttcaggaggt attatgcctc tacattatag
     4381 tagctttaag attgcagaat ggataatgac tttagaggcc ttgcacccta atcggattga
     4441 tttaggtatt ggcaactctt taggaactac gttagttcaa agagctctat caagtatcca
     4501 ttgcaaagac agctatagtc aggttgtcac agaattatac cagtatctta atcctgatca
     4561 tttgtcacca ctaccgattt ttgttaaccc taggggtaat acttaccctc agatttggac
     4621 gctaagcaat agtttggaga cagcagaact ggctggtcaa ttaggtttag gctatacctt
     4681 tggtattttc ccctatatac caaaagatcc tataacagaa gctaaaagag tatcggcaca
     4741 ttatagaaag gcttttaggc cttcaaagct attaaaaatc cccaagttaa tattagcagt
     4801 ttttattgtc ctttctgata ccgacgagga agcagaagct ttggctaaac cgttggacat
     4861 ttggatgctt ggtcagcaag attttaatga atttaagacc taccctgatg ttgaggaggc
     4921 tagaaactat cacttaacag agaaacaaag agaagccatt gcagcaaatc gtagtcgcat
     4981 ggttattgga tcacctcaca cagtcaagaa acaattggat aggctgatag aagcttgtca
     5041 agctgacgaa ttattagcca tccccttagt tcctgaattt gccaatagac aacgaacctt
     5101 agagctgcta gcagacttat acgtgaccat ttaaccttac caaaaacaat tccctaaact
     5161 gaaaggaaag aaacaatgaa aaaaattgca aattacttat taattgagaa aacagatgac
     5221 cgctatacca ttagcatgac accagaatta caagacgata ttggtactat tggttacgct
     5281 gaatttacgg ataacgacca ccttgccgtt gatgacatta tcttaaatct agaggcttct
     5341 aagaccgtca tgtctgtctt gtcaccattg gctggggcag ttgttgagcg caatgaggca
     5401 gccacactaa caccaaccct tttgaattca gaaaaagctg aggaaaattg gattgttgtg
     5461 ctaacagatg ttgatcaagc agcttttgat gccttagaag atgcctagtt catttgattt
     5521 attaggggaa atgattgatc tgttgcaaac agagcagctt actagttctt gggcctgtcc
     5581 cttgcctaat gctttaacaa agcgacaaga cttgtggcgg gcgctcatta accagcgccc
     5641 tgctttgcct ttatcaaaag attacttgaa tctggaagat gcttatctag atgattggcg
     5701 tgcttctttt gttcctgtat cagtcaagga ctgccaaaaa actaattata ccagtctttt
     5761 tctttaccat ggggatatta ggtaccttgc tgtggatgct attgtcaatg ctgctaatag
     5821 tgagttattg ggatgtttta tccctaacca tggctgtatt gataatgcga ttcatacgtt
     5881 tgccggaagt cgtttacgct tagcttgtca ggcaatcatg actgaacaag gccgtaagga
     5941 agccattggc caagcaaagc ttaccagtgc ttatcatcta ccagcctctt atattattca
     6001 taccgtaggg ccaagaatta ccaaaggacg acacgtttct ccgattcgag ctgatttgtt
     6061 agcacgttgt tatcgttcta gtcttgattt ggcggttaaa gcaggattga ctagcctagc
     6121 tttttgctct atttcaacag gagaatttgg ctttcctaaa aaagaagccg ctcagattgc
     6181 cattaagaca gttttaaaat ggcaagcaga acatccagaa tctaagactt taactatcat
     6241 ttttaatacc tttacatcag aagacaaggc cttatacgac acttatttgc aaaaggagaa
     6301 taactgtgag taattggaca acctatcctc agaaaaatct cacccaggca gaacaactgg
     6361 cccaattaat caaagaagca gatgcacttg tagttggtat tggagctggc atgtctgcag
     6421 ctgatggttt tacttatatc gggccgcgtt ttgagacggc attcccagat tttattgcta
     6481 agtatcagtt tttggatatg ctgcaagcta gcctttttga ttttgaagat tggcaagaat
     6541 attgggcttt tcaaagtcgc tttgtcgctt tgaattatct tgatcagccc gtaggccagt
     6601 cttatcttga tttaaaagag attttggaaa ccaaagacta tcatattatt actactaatg
     6661 ctgataatgc tttttgggtg gctggttatg atcctcataa catttttcac attcaaggag
     6721 aatatggttt gtggcaatgt agccagcact gccatcagca aacttataag gacgatacgg
     6781 tcatccgtca aatgatcgca gagcaaaaaa acatgaaagt cccaggtcaa ctgattcctc
     6841 attgccctga atgtgaggcg ccttttgaaa tcaataagcg taatgaagaa aaaggaatgg
     6901 ttgaagatgc tgactttcat gcccaaaaag cacgttatga ggctttttta tcagaacata
     6961 aagagggtaa agtgctttat ctagaaattg gtgtgggaca tacgacgcct cagtttatca
     7021 aacacccctt ttggaaacgt gtctcagaaa accctaatgc cttattcgtt accttgaatc
     7081 acaaacatta ccgcatccct ctgtcaataa ggcgacaaag cctagaactg acagaacaca
     7141 ttgcacaatt gattagcgcc acaaaaacca tttaccaaaa atcgtaacca aagccaatag
     7201 acggttaatt aagagatata gaaggagact aacatgtacc ttattgaacc gattcgtaac
     7261 ggtaaacgta tcaccgatgg agctgttgct ctagccatgc aagtttatgt tcaagaaaat
     7321 ctatttttag atgatgatat tttatttccc tactattgtg atcccaaagt tgaaattgga
     7381 aaattccaga atgcggttgt agaaactaat caagagtatt taaaagagca tcatatccct
     7441 gttgttcgcc gagatactgg cggtggtgcc gtttatgtag attctggtgc tgttaatatt
     7501 tgctacttga ttaatgataa tggtattttt ggtgatttta aaagaaccta tcagcccgct
     7561 attgaagccc ttcatcattt gggagcaaca gaggttgaaa tgtctggacg gaatgacttg
     7621 gttattgatg ggaaaaaggt atctggcgct gcgatgacta ttgctaatgg ccgtgtttat
     7681 ggggggtatt cccttttgct agatgtggac tttgaagcca tggaaaaagc attaaaacct
     7741 aatcgcaaaa aaattgaatc aaaaggcatc aggtcagttc gtagtcgcgt tggaaacatc
     7801 agagaacatt tagcaccaca ataccaaggt attacgattg aagagttcaa agatttaatg
     7861 gtttgccaat tgttacagat tgagacgatt agccaagcta agcgttatga tttaacggaa
     7921 aaagactggc aacaaattga tgctttaaca gagcgtaaat atcacaattg ggaatggaat
     7981 tatggcaacg cgcctcaata ccgttaccat agagatggcc gttttaccgg tggaacagta
     8041 gatattcatt tagatattaa aaaaggctat attgcagctt gtcgcattta cggagatttt
     8101 tttggcaagg cagacattgc agaacttgaa ggtcacttga taggaacacg aatggaaaaa
     8161 gaggatgtct tagctaccct caatgcaatt gatttagccc cttatttagg tgcgattaca
     8221 gcagaagaat taggagattt gatattttct tgataaaaaa gatacctcag accggtaaag
     8281 tgcgattaaa aacgaacaat aattgtttta attcaaataa atgtatgtat gatatgaatt
     8341 gtgatataat gacgtcatta cgtaaaattg aggagtcatc atgaaatcag atatcgaaat
     8401 tgcccaaagt gtcgccctac aaccaatcat tgacattgtt aaaaaggttg gaatagacgg
     8461 cgatgacatt gagctttatg gcaaatataa ggctaaattg tcttttgaga aaatgaaagc
     8521 tgtcgaagct aatgagcctg gaaaattact cttggtaact gctattaacc ctactccagc
     8581 gggagaagga aaatcaacca tgtctattgg actggcagat gccttaaatc aaatgggaaa
     8641 aaaaaccatg cttgccttac gtgaaccctc ccttggtcct gttatgggga ttaaaggcgg
     8701 ggcagcaggt ggtggttatg cccaagtatt gcctatggaa gacattaatc ttcactttac
     8761 tggtgacatg catgccatta caaccgctaa taatgcatta tctgctttga ttgacaatca
     8821 tttgcagcaa ggaaacgatt taggaattga tcctcgccgc attatttgga agcgcgtgtt
     8881 ggaccttaat gatcgtgctc ttcgtcaggt aattgttggc ttaggcagtc cagtgaatgg
     8941 tgtgccacgc gaagatgggt ttgatatcac agtagcttct gaaatcatgg caattttatg
     9001 tttggcaact gacttgaaag atctaaagaa acgtcttgct gatatcgtcg tggcttatac
     9061 ctatgatcgt aagcctgtct atgtgcgtga cttgaaggtt gaaggagcat tgacattaat
     9121 tctaaaagat gctatcaagc caaacttggt gcagactatt tatggaacac cagctcttat
     9181 tcatgggggg ccatttgcta atattgctca tggttgtaat tctgtccttg ctacctcaac
     9241 agctttgcga ttagcagatt acactgttac cgaagcagga ttcggggccg acctaggagc
     9301 tgaaaaattc ctaaatatca aagtacctaa tcttcctaaa gcaccagatg ctatcgtcat
     9361 tgttgcaacc ttgcgcgccc ttaaaatgca tggaggtgtc gccaaatcag atttagccgc
     9421 tgaaaattgt gaggctgttc gcttaggatt tgccaactta aaacgtcacg tggaaaacat
     9481 gcgccaattc aaagttccag ttgtggtggc tatcaatgaa tttgtagcag ataccgaggc
     9541 tgaaattgct accttaaaag cgctttgtga agagattaag gttccagtag aattagccag
     9601 tgtttgggca aatggagctg agggaggctt agcattagct aaaacagtgg ttcgtgtgat
     9661 tgatcaagaa gctgctgact acaaacgtct ctattcagat gaggatactt tggaagaaaa
     9721 agtgatcaat atcgttaccc agatttatgg tggcaaagct gttcaatttg gccccaaggc
     9781 caaaacacaa ttgaagcagt tcacagaatt cggttgggat aaattgcctg tttgtatggc
     9841 taagacccaa tacagttttt cagataaccc atccttgctt ggagcgccaa ccgattttga
     9901 tattaccatt cgtgaatttg ttcctaaaac aggggcaggt tttattgttg gtttaacagg
     9961 tgatgtgatg accatgcctg gtttgcctaa agttccagct gccatggcaa tggacgtagc
    10021 agaaaatggc acagccctcg gtctctttta ggaaaaattg ctcgaataat tcgagcaagc
    10081 gctcctttct tcccgcttta ctgtgatata ataaggtaac tgagattttg gagggatgat
    10141 tatcaaaaag aaagcaaaag taaaatattt gctccataaa gggaaacatg gatttttaag
    10201 aggaattttt agtagaacaa caatcattgt attattaatt attttgcaat tagttttctt
    10261 atttcaatct tatgcctgga tggagcagta ccgtgtttgg ataacgattc ttgaaagtgt
    10321 ctttgctatt actattgttt tatatttagt taatagtgat atggatgcta tttcaagaat
    10381 gacgtggtta attttgatta tgattgctcc attactaggg tcactgtttt taatttatac
    10441 aaaattggat tggggttaca gagggctaaa acaaagaata aatcatcttg tagatctgtc
    10501 tgcaccttac cttagcgatg atgacgctat attagaggta ttaaaggata gcacatcaac
    10561 cacatatcat ttagtacagt acttagaaag aagtcgtggc aactttccga tttataataa
    10621 tacaagggta acttacttcc caacaggtga aacttttttt gatagtttaa aagaacagtt
    10681 attcttagct aaaaagtaca tttttcttga attttttatt attgcagaag gccaaatgtg
    10741 gggagaaatc cttagtattc tagaaaaaaa agtcagtgaa ggtgtagaag ttagggtgtt
    10801 gtttgatggc atgaacgaac tatcaacgct atcatcagat tacgccaaga gattagaaca
    10861 aatagggatt aaagctaaat catttttacc gatttcaccc tttatctcta cctattacaa
    10921 ttatcgagat caccgaaaaa ttgtcgttat tgatggggag gtatcattca ctggaggtat
    10981 taatctagca gatgagtaca ttaatgaagt agagcgtttt ggccactgga aagatgctgg
    11041 tttaatgctt gagggtgaag caacagacag ctttttaatt ttgtttttac aaatgtggtc
    11101 tatcacagaa aaagaactga ttattgatcc ttatctttca gatcattctt taaaacttcc
    11161 ttcagatggc tatgttattc cctacggtga ttccccgctt gatactgata aaataggtaa
    11221 aaatgtttat atagacattt taaatcatgc taaagagtac gtttatatca tgacacctta
    11281 ccttatttta gatagcgaga tggaacacgc tttacgattt gcatcagaac gtggagtaga
    11341 tatccgaatt attatgccag gagtgccaga taagggggta ccatatgcct tagctaaaac
    11401 ctattataaa gctttgatga cttctggagt aaaaatttac gagtatcaac cagggttcgt
    11461 tcattcaaag gtatttatct ctgataatac aaaagcagtt gtcggaacaa ttaacttaga
    11521 ctatcgtagc ctttatcatc attttgaatg cgcgacttat ttatatcgtg tgtcagttat
    11581 tgctgatatt gtgaatgact ttaatgaagc tcaaaagcaa tcactattga tgacatcaga
    11641 tcatttgacg caacgtcctt ggtatcaaaa attgatagga ttattagtac gaacaattgc
    11701 ccctctcttg taattttcgt aaaatatgtt ataatatctg tatgcgatga gtcgattgtg
    11761 actgcaaagt cacttttgcg caggggaggt cattaaggcg ggaagccgac tttgataatc
    11821 tgtgtgaacc attttatcac aatcttattt attaagaatg ccccttggat tgagatattc
    11881 tcaatccttt ttttaatttt attatcttag ttaaaattca gaatgtatca gtgattactg
    11941 tgtttcaaaa taagaaagta gtttttcggg tatgagtgct atgacttgac aaatgctaat
    12001 aacatttgca taatgtaagt agagatgaaa acttatgaaa agagatgatc ttatgcaaat
    12061 tattaagcgt gatggccacg ttgctgattt tgatccagat aaaatttatc aagcaattat
    12121 taaagcagca aggacggtct atgttattga tgacacgtgg cgacaaaact taacacatgt
    12181 cactaaaaaa gtagttattg atttagaaga agcacatgta gaacgtccta ctatcaatat
    12241 ggtccaatcc cttgtggaga atcgtttgat ggatgctgga tatattacca ttgcagaaca
    12301 ttacatttct tatcgtcttc agcgagattt agagcgcaat ggctatggtg ataagattgt
    12361 ggttcacttg cgttttgaac aaataaaata aaaacgcttc taatggcatt ttagtgtctt
    12421 tgaggggata aggtttaaat acctaacaga taaaaatctg ttaggtattt ttttgctact
    12481 attaggtgta ttctatatca catagaactt aagcattcaa gctttaattt aactttctgt
    12541 ttattttttg ctacattttg ggaaactccc ctcctttctt agt
//
LOCUS       DATUYR010000045        12176 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.045, whole genome shotgun sequence.
ACCESSION   DATUYR010000045 DATUYR010000000
VERSION     DATUYR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.045"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(113..418)
                     /locus_tag="VCI33_001245"
     CDS             complement(113..418)
                     /locus_tag="VCI33_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEP2832170.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(430..849)
                     /gene="ruvX"
                     /locus_tag="VCI33_001246"
     CDS             complement(430..849)
                     /gene="ruvX"
                     /locus_tag="VCI33_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEP2832171.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(846..1115)
                     /locus_tag="VCI33_001247"
     CDS             complement(846..1115)
                     /locus_tag="VCI33_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEP2832172.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1229..1627)
                     /gene="spx"
                     /locus_tag="VCI33_001248"
     CDS             complement(1229..1627)
                     /gene="spx"
                     /locus_tag="VCI33_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEP2832173.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            complement(1918..3054)
                     /gene="recA"
                     /locus_tag="VCI33_001249"
     CDS             complement(1918..3054)
                     /gene="recA"
                     /locus_tag="VCI33_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEP2832174.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPELFD
                     EIDRKVRVKFGLLEESEEESAMVVASEEIDDLALDLDNGIEIED"
     gene            complement(3143..4414)
                     /locus_tag="VCI33_001250"
     CDS             complement(3143..4414)
                     /locus_tag="VCI33_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEP2832175.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            complement(4483..5043)
                     /locus_tag="VCI33_001251"
     CDS             complement(4483..5043)
                     /locus_tag="VCI33_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEP2832176.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVAAFTSEEMADALENPSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            complement(5053..5649)
                     /gene="ruvA"
                     /locus_tag="VCI33_001252"
     CDS             complement(5053..5649)
                     /gene="ruvA"
                     /locus_tag="VCI33_001252"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEP2832177.1"
                     /translation="MYDYIKGQLTKITAKYIVVETNGLGYIINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGNTKARSNKAGNTQLDEAIEAL
                     LALGYKAAELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            complement(5651..6871)
                     /locus_tag="VCI33_001253"
     CDS             complement(5651..6871)
                     /locus_tag="VCI33_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2832178.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFVGILLVEIASNFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFHDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            complement(6882..8864)
                     /gene="mutL"
                     /locus_tag="VCI33_001254"
     CDS             complement(6882..8864)
                     /gene="mutL"
                     /locus_tag="VCI33_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEP2832179.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQFRGLYYDPQKNDFFVKESAVSEKIPETDFYFGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNTFIETDHPNLDLKNRQKLSQMLTRLE
                     NEEQSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            complement(8959..10110)
                     /locus_tag="VCI33_001255"
     CDS             complement(8959..10110)
                     /locus_tag="VCI33_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP2832180.1"
                     /translation="MIMKITEHKKKNGTIVYRASIYLGIDQMTGKRVKTSITGRTRKE
                     VNQKAKHAQFDFLSNGSTIKRKVVIKTFKELSHLWLETYKLTVKPQTYDATVTRLNRH
                     IMPTLGNMKVDKITASDIQMLINRLSKYYVNYTAVRSVIRKVLQQGVLLGLIDYNSAR
                     DIILPRKQPNAKKKVKFIDPSDLKSFLEHLETSQHKRYNLYFDAVLYQLLLSTGLRIG
                     EACALEWGDIDLENGTIAINKTYNKNLKFLSTAKTQSGNRVISVDKKTLRSLKLYQMR
                     QRQLFNEVGARVSEVVFATPTRKYFNASVRQSALDTRCKEAGIERFTFHAFRHTHASL
                     LLNAGISYKELQYRLGHANISMTLDTYGHLSKDKEKEAVLYYEKAMNNL"
     gene            10359..10502
                     /locus_tag="VCI33_001256"
     CDS             10359..10502
                     /locus_tag="VCI33_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP2832181.1"
                     /translation="MSGGGAYVCKSQKPKERRRQGLSVYETLTLMIAFGTLIVAIMNN
                     KNK"
     gene            complement(11364..12131)
                     /locus_tag="VCI33_001257"
     CDS             complement(11364..12131)
                     /locus_tag="VCI33_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2832182.1"
                     /translation="MAKNSPQDLINREIFSTNLNMLMAKKNIKQIDIHNKLGIPKSTI
                     TGYVKGRSLPTAGNVQKLADFFGVLKSDIDPRFDSNNIETNSNIIPSTLQKVTSTLSQ
                     LEHKRQLNVLDYAETQLEQQNTVEEPQATYYTYNYYDHAASAGTGQYLNDVQVETIEL
                     PVDYDADFVIPVYGDSMEPEYHSGDYVFIKLSINLSDGDIGVFEYYGDAYIKQLVIND
                     SGAFLHSLNDKYDDILIDRDSDFRIIGEVIGSFTSKS"
BASE COUNT         3713 a         2510 c         2078 g         3875 t
ORIGIN      
        1 ataaatttat catttaattc ttttatcatt atcacctcct gcttatttat tcgaaaataa
       61 cattaaaaaa atagccctca cggctacttt ttaacatagt gtcactaaca atttagttct
      121 catctagaaa actattaaat acctcttcaa tcatatccca ttcagcatct gaatcctcag
      181 gaatcggttg taaatcccct tcagtaccat cttcattttc agtaaatgaa taggcttgga
      241 tttcaatttc accagactca tcttcttcag atcccgctgg aaccaagaga acataatttt
      301 taccaaactc ttcacgaccg tcaattgtta ataaaatttc aaacaaggtt tcatttcctt
      361 gttcatccac aagtgtgata acttcgtgtt ggtggtcatt ttcatgatta tgtgtcattt
      421 tttatctcct taaaaattcc gatttaaata gttctgtaaa atgagttgtg cagctaattt
      481 atcaatgact ttttttcgct taccacgact aatgtcagct tgctcaataa gcatacgctc
      541 tgcctcaaca gtcgtcaatc gttcatcttg ataatggact ggaagtccaa agaggtgctc
      601 tatgtggttt ccataagtta tacttgcatc aacacgtgga ccattggtgt tattcatatt
      661 ttttggcaat ccaataacaa actgttcaac ttggtactgc ttaacaagct cttctaaacg
      721 tgtaaagcca aattctgctt tttcctcatc aatcttgata atttcaagtc cttgggctgt
      781 aaagccaaga ggatcactaa tagctacacc aaccgttttc gagccaacat caagtcccat
      841 tattctcatt taacatcaat tccatttcct tgtaaatagt agcgaacaag ttcttctaca
      901 atttcatcac gttcatattt acgaatctga tttcgagcat cattataacg aggaacataa
      961 gctgggtccc cactcaagac ataaccaaca atttgattaa ttggattata acccttttca
     1021 tcaagtgaat gatagactgc tgttaatgtt tcactaattt gtcgtttgtc gccatcatcc
     1081 aacttaaagc ggactgtttc atctgtaaat cccatactta caccttcttt ctaaaattaa
     1141 gtgctatgct ttattatagc ttatttctat taaataaaca agaatttcaa gcgaagaagg
     1201 tatttaaaaa aaagctctac aagagctttt agagtgcagc acgtaatcgt gcttcagtat
     1261 tttcaatatt acgaatagag cgaggtaaaa aagcacgaat atcgtcttct ttgtagccta
     1321 cttgcagacg tttatcatca atcaaaatgg gacttttaag gatgcgcggg ttatcttgaa
     1381 tcaaatcaat aacctcacta acacttagtt cttcaatatc gcaatcgaga gctttggcat
     1441 aacgattttt agatgaaaca atgctctcca ctccattttc ggtttttgat aaaattgcta
     1501 aaatttcttc tttagttagc ggttcttttc ctaagttctg ttctttataa gcaagcttat
     1561 gggcatttag ccaagttttg gctttcttgc agctcgtaca acttgaaatc gtgtaaattt
     1621 taatcatgtt gctgactcac tttctatttc gtgatagctt tattgtagca cagtttctta
     1681 tctataaata taggtctttg gacctatttt tttaatttta aagatattat cttggtaaaa
     1741 taaggttttt cacctgtatt cccttgaatt attagttatt ttaattttat atcagttaac
     1801 ctaagttctg ctctctcaca tctttttaaa tgatggtttc tattcaccca cacacctatg
     1861 aatagtagtc ctactaacct ttaaaaatac attttaaaaa gccctgaagg gctcttttta
     1921 atcttcaatt tcaataccat tatctaaatc taaagcaaga tcatcgattt cttctgatgc
     1981 tactaccata gcagattctt cttcgctttc ttcaagcaaa ccaaatttaa cacgtacttt
     2041 acggtcgatt tcatcaaaca attctggatg atcggccaaa taacgcttag cattttcaga
     2101 accttggcca atcttctcac cattataaga gaaccaagca cctgcttttt ggataatgtc
     2161 caaatcagaa gcaattttca caagctcccc tgtacgagaa ataccttccc catacatgat
     2221 ttcaacttct gctaccttaa atggcggagc gaccttgttt ttaacaacct tgattttggt
     2281 ctccttacca atgctgctat ctttttggtc accagttcct ttaatttgag ttgttccacg
     2341 cacatccaac cgaacagaag catagaattt caaagctcga ccacctggtg ttgtttcagg
     2401 atttccaaac atgacaccaa ctttttcacg caattggtta ataaagatag cgatagtttt
     2461 tgttttatta atagaagctg ataatttacg catagcctga ctcatcatac gtgcttgcaa
     2521 tccgacatgg ctatcgccaa tatcaccatc aatttcagca cgtggcacta aagctgctac
     2581 tgaatcgaca acaaccagat caaccgcacc agaatcaatc aatttacctg caatttcaag
     2641 tccttgttct ccagaatctg gttgagacaa gaggagttca tcaatattaa ccccaagcgc
     2701 agcagcataa gctggatcaa gcgcatgctc ggcatcgata aaggctgcga ttccaccttc
     2761 tttttgagct tgtgctacag catgtaaagc cacagtcgtt ttaccggaag actctggacc
     2821 atagatttcg atgatacgtc ctttaggata accaccagct ccaagcgcaa tatcaagagc
     2881 tagacttcct gaactcataa cctgaacttt ttgctctgca cgttctccta atcgcatcac
     2941 tgcgccctta ccaaaatctt tttcaatatt tttcaaagca tcatcaaggg ctttgcgacg
     3001 ttcgtcacca aacttcttag tgatttcttc attctttttt aatttttttg ccaatacttt
     3061 ctcctctttt ttaacaaacc atcgtcagca ggattaatct acataatcgt aaaccatatc
     3121 ctactatagc tactaaataa cattatagca aattctcaag ttttaataaa gttttacgga
     3181 ccatattaaa ggcatgtaaa gtagcgatat agcgcacatc caaacgactt cgcccgctaa
     3241 tcaaaacctt tattgattct accttatttt gagtggcaag gccaatgaaa actgtacctg
     3301 caggctgttc ctccaacata tctggtccgg caacacctgt taaacttacc ccaatatcag
     3361 caccagtcaa taaccttgct tgctccgcca tcccctcggc tgtataagca ctaacaacgc
     3421 catgcgattt caactcctct aaaggaaggc ctagcatttt cgctttttct tccatgctat
     3481 aagtcacaaa gcctccattg aatacttgag aagctcctgg aaaactcgcc aaagttgact
     3541 gaaataatcc cgcggttaga ctttctgctg ctgtaattgt cttatcatat tttactaaga
     3601 gctcaaatgt ctcacgcgct aaggaattat cctccccata gccataaaag acatcagcta
     3661 agggttgacc ttcaagagtt tttcgggata gtagctgcgc ttctagctga cctaactttt
     3721 tatctgccag agcttggttt tcagtttttg ttgataagcg aagagtcact tcgccagtct
     3781 tagcatacgg agcaatggtt ggatcagttt gattctcaat aaaatctgac aagactgtta
     3841 ccaactgact ttccccaata ccaaaaaagc gtagtacctt tgaatacaat gtactgtatt
     3901 gttttgacag aagtggtacc aattcttcat ttaccatcgg cttcaattca cttggaggac
     3961 ccggtaagac aacataacta ataccatcga ctgtgatgaa cccaccaacc gcaagaccag
     4021 ttttatttgg caaagggatt gacccttcaa tcacttgtgc ctgtcgctca ttatttggtg
     4081 tccgtgatga aggcttgcgc ttagcaaaaa agtcatctag tttctgacaa gcttgctcat
     4141 cataaaccaa gtctttccta aggtactttg ctaaagtttg tttggttaaa tcatctttcg
     4201 ttggaccaag gccaccacat aaaattacca agtcactacg ctgactagca gttgtaatca
     4261 ctgaaagtaa acgctcctcg ttgtccccaa cagccgtttg aaaatagaca tcaataccta
     4321 gctctgccat tttttccgac agaaattgag cattggtatt cacaatttga ccagtcaaaa
     4381 tttcggtacc tactgcaatc agttcagctt tcatttcccc acctacttat ctattcgtaa
     4441 tctgatctca ttttatcaaa aaatcttcca attcacgaag aattagaaga ttctattgaa
     4501 aacacaagcc tcttcatggt catttaccag acccgatgct tgcataaaag aataaaccgt
     4561 tgtaggccct aaaaatttaa atcctctttt ctttaaatcc ttagctaagc gaatagataa
     4621 ttctgtttga gcaggaacaa gattctcttg attgacaagg ttgttaattg gctttccccc
     4681 aacaaaattc cagagataag ttgaaaaact accaaactct tcttgaattt tctgtactgc
     4741 gatagcattg ttgacagttg ctgctagctt tagcttgtgg cgaatgatgg atggattctc
     4801 taaagcgtca gccatttcct ccgaggtaaa ggctgcaacc gatgcaatgt cgtaatgatg
     4861 aaaaacagtt ctgaaagctt ggcgtttttt caaaacagtc agccaggata agcctgactg
     4921 gtaactttca aggcagagta attcaaaaaa atctctatca tcatgtaggg gttgacccca
     4981 ttctaaatcg tggtaatcac agtaaagctg gttatcctta ggaacccatg aacaacgttt
     5041 catgtgaaac acctagcctt tcattaatag tttaagggct gatttgatgt attgttcggc
     5101 cgtttcagag gttccttcaa agaaggcacg aattttcttc aattccgctg ccttataacc
     5161 gagggcaagg agggcttcaa tcgcttcgtc cagttgagta ttgcctgctt tattgctgcg
     5221 cgctttggta ttaccagtct cttgtggagc ctccacaaat ttgccagcta agtcaagaac
     5281 catttgctgc gctgtttttt taccaatttt aggaaatttc attaggtact taatgtcact
     5341 gttatcaatg gcattcacaa gtccctcatt atcatcaact gccacaatag caagagctgt
     5401 tgtggggcca atgccagata cagaaattaa tttcagaaaa acatcttttt cgtcttccgt
     5461 atgaaaccca aacaacaagt gagcatcctc acgaatcact tgatgcaggt aaatagttac
     5521 cagttggttg acactatctg taaagctata aggattggct acattgataa tatagcctag
     5581 tccatttgtt tcaacgacaa tgtattttgc cgtaattttg gtcaattgac ctttaatata
     5641 atcgtacata ctacaccttt ttcatttttg cagaaacaac cgtaaaataa atccctagca
     5701 aagcaatgac tacaaataag cattgcacac ctaaaggacc tgtaaaatgg ctgacagata
     5761 ctagtagact agccaaaata gcacctaggg gttgtgccat tgacacaaaa cctgtataag
     5821 atcctatttg ggaatgatcc atcatatcag cacgtaggac ttgactagca ggaacattta
     5881 tcatttcccc aaaagttaac aaaacaactg atagccaaat agcatagaac tgcgtaaagg
     5941 taaatgccaa gagcatccct agagtaaata gtaaagaacc aaccacaagc tgaggtaata
     6001 gtttccattt ttccgttaat ttatttacta ctgtcatgaa aaggacaatc agcaatgtat
     6061 tagttaaaac cattaatgat aacattttag aactagttac ttggaaacct aacacagccg
     6121 ttggctgaaa atacagtttc aaatggactg gcacatagtt atccatctgc atccaaatac
     6181 taccagaaaa gatggcacct aaggtaaaca agacaaaggc acgatcatga aacacctgac
     6241 ggtagttctg aaaactcgcc agtaatcctt taccatggtc aaaagcgtgt gtttctggtc
     6301 tagtctcatc aaaatagtag taagcaacgc caaaacaaag tacattaacg agtaataagg
     6361 ctactaacaa ggctaaaaaa tgatggtcat aaaaaagccc agatagccct gcaccaaaca
     6421 tgacggcaat attgataaac caataattga tggtgtaaac aaatcgacga ttactctcat
     6481 cagtcaaatc aattaacata gcttcatagg caggaccata aaaattagaa gcaatctcta
     6541 ccaataaaat acccacaaag gttaaccaag gaatagctgc attaggcaaa ttagctaaaa
     6601 tagtcagaaa ccagcctagt gttgttccta ctgacccaat cataatgact tttttgcgac
     6661 caagagcatc tgacagatgc ccaccgtata aagttccaac aaatcccatc aaactggtaa
     6721 tcatcattaa gaggcctgtc caaaacgtac caaagtaagt cgtataatac atggccataa
     6781 agggaaaaat actactgcct aaggtaatgg tcacaaagcg taccagttgc ctcagctgaa
     6841 tctgcttagg aagatttaaa aactcttgca tctcatcctc tctaatattt tcctagctct
     6901 cgcaggctag tgtgattttc ttggatacga cggaacattt tttccatatc tgccttacta
     6961 aaattaatca atacgggtct accatgaggg cagttataag gattttgaca ttgggccaat
     7021 tgcagtagca gatttcttgc tgaataatca tccaagctat gatttgcctt gatagatcgt
     7081 ttacaactca tcataatagc taactctgct cggtaagttt taatagatac ctcattggtt
     7141 agaagtaaca tgtcacacat ttcatagaca ccagatgcaa tctcttcttc tttcatccag
     7201 ataggatgct ccctcaaaat gaaagtattg tgcccataaa cttctaagaa gataccaact
     7261 tcatttaaga gtgccatttt ctcctgtaag ttaatgaaat cagaaccaga aaactcaaac
     7321 aaatagggca ccaacaattg ctgtaaacta ctgtcaacct cacctatctt atcacgataa
     7381 tattcgtatt taacccgctc ttgagcagca tgctgatcga taataaataa gccatcttta
     7441 ccttgagcaa agagataagt tccgtgcatc tgaccaaaat aatccaattc gggaaacact
     7501 gattgctctt cattttccaa acgggttagc atttgagata acttttgtct gttttttaag
     7561 tcaagattag gatgatcagt ctcgataaag gtattctggg gacgacttgc gtgtttaacc
     7621 gaagaaggtc ctataacttc ttctgagtgg ggtaacagct ctgctttttc aacctttaca
     7681 ctgttgtcaa cagcgccgaa ataaaaatca gtttcaggta ttttttccga gacagccgac
     7741 tctttgacaa aaaagtcatt cttttgagga tcataataaa gtcccctaaa ctgtaaagga
     7801 agttgtgttt gctctggttt agagaaatgt cgtgtgcttg atttggctaa gttttccaga
     7861 gcatctggaa tcaaatcttg ttccttaaga ctttcagaaa tcgccgtact aattaaagcc
     7921 atcaactcac gctcttttga aatacgaacc tcttgttttg tgggatgaac attgacatcg
     7981 gccaaatagg gatcaatctg aatatcaatc acaacaattg ggaaacgtcc aaccatgagc
     8041 ttagaaccgt aaccatcaag aattgctcga ttaagcaaga aattcttgat gtaacgccca
     8101 ttaaccaaaa tcgtcatgta attacggttg gcgcgtgtca actctggcaa gctaacatag
     8161 ccggaaactt caaaatccaa atcagcatta gagatagcca gcattttttt ggtagtattc
     8221 aaaccataga tccctgcaat tgcttggcgt aaatcgccag ttcctgatgt ctgagttaat
     8281 tggcgaccat cactaatcag tgtgaaagca acttctggat gtgccaaact caaccggttg
     8341 accacatcta caatgtgagc taattctgcc tgtaaacttt tcatatattt gagacgagca
     8401 ggcgtgttgt aaaaaaggtt ttcaacctta attttggttc cagtaggagt tgagatcgct
     8461 tcaagtgtct caatttcccc accagtagct ataagaagag aaccgtgagt gacttctttt
     8521 gttgctgttt ttatcgtgat tttactgata gaggcaacag acggtaaagc ctctccacgg
     8581 aagccaagtg ttctaattct aaacaaatca ctctgactct taattttact agtagcgtgg
     8641 cgacgcagac ttaaaggtaa atcttcatga gacattccct caccgttgtc tgtaacctgt
     8701 atcatcttaa ggccagactc ttcaatttca acggtaatct ggctactttt agcatcaata
     8761 gcattctcaa ccaattcctt gacaacactc gctggccttt ctacaacttc accagctgca
     8821 atttggttgg cgagaacttc cggtaattca ataatgtttg tcataatttt gtttttaaca
     8881 ctttctaaac gttgatataa caaggtttca ttaggtttac acctctcaaa atattaattt
     8941 gtggactttt ttgtggactt ataaattatt catagccttt tcataatata aaacagcttc
     9001 tttttctttg tccttagaaa gatggccata ggtatccaaa gtcatgctga tattcgcatg
     9061 tcctagacgg tactgaagtt ccttataact aatacctgcg ttcagcaata aactagcgtg
     9121 agtgtgtcta aaagcgtgaa aggtaaagcg ttcaatccct gcttccttac acctagtatc
     9181 taaagcgctt tgtctaaccg aagcattaaa atactttcgt gttggtgtgg caaacactac
     9241 ctccgacaca cgcgcaccaa cctcattaaa taattgtcgc tgtctcattt gatagagctt
     9301 taggcttcta agggtctttt tatcaacact aatcactcta ttgcctgact gggttttagc
     9361 tgtactcaaa aacttcaaat ttttattgta agtcttatta atggctattg taccattttc
     9421 taggtcaata tctccccact ctaatgcaca ggcttcgcct atcctcaagc cagtggataa
     9481 taaaagttgg tagagaactg catcaaagta aaggttatag cgtttgtgtt gactagtttc
     9541 taaatgttct aaaaaagatt tcaaatcaga cggatcaata aacttaactt ttttcttagc
     9601 gtttggctgc ttccttggaa ggataatatc tcttgctgag ttataatcta ttagccctag
     9661 caatactcct tgttggagaa cttttcggat gactgaacgt accgcagtat aattgacgta
     9721 atatttagat aatctattaa tcagcatttg aatatcacta gcggttatct tatcaacctt
     9781 catattgccc agagttggca taatatgtcg attaagtcta gtaacagtag catcataagt
     9841 ttgaggcttt actgttaact tataggtttc aagccataaa tgactaagtt ctttaaatgt
     9901 tttaatcaca acttttcttt taattgtaga tccattagat aggaagtcaa actgcgcgtg
     9961 cttggctttt tgattaactt cttttcttgt tcttcctgtg atgcttgttt ttactctctt
    10021 acctgtcatt tggtcaatgc ctagataaat actagcacga taaacaattg taccgttttt
    10081 cttcttatgt tctgttattt tcatgatcat aaacctttcc atcagcaggc aagctattat
    10141 taaaagggtt ttagattagt tcatatcatg gtataataat ggtatcgggt aaatatccga
    10201 aatattataa aagaaaatca tggtataata ttagtctttt taagaccttg cgagcgtgga
    10261 aaacttgcgt ggtctttttt gtttggtttt gtaaggtaca gagagtttca gaagaagttt
    10321 tgcatgtcta aaaactaggt caaggtagtc gtagccttat gtctggaggt ggtgcttatg
    10381 tatgtaagtc acagaaacca aaagaaagga gacgacaagg tttgtcagtt tatgaaacgc
    10441 ttactttgat gattgccttt ggtactctga tagtagctat catgaataac aagaacaaat
    10501 agaaaaaccg tcccacactt tagccaatcg tggacggttt atagaaaata taaacaaata
    10561 cggtaaggct gccgtcttaa acggtgctac cacaggagat tgtttgcgca atctcctttt
    10621 tctatgtcca ttatagcttt gtttgataaa gaagtcaaaa gggtgtagtt agaaagtaca
    10681 gtctttagcg ctcttaacat tccttagtat taccaaaatg aataaaaata attttatgca
    10741 agaatttata atactttatg caaccactga tcacgcgcag atagtttttt taattaccgc
    10801 cgacaaaaag ttcagaaaag ttcagattat acctcaattt ttgccaagtt ttgagggtct
    10861 tgtaattaaa gaaaattaaa ggcgtatcaa ggtatttgcg cgtaggtaaa gatgataaat
    10921 ttggcattta gacatgaaaa ttgaacaggt cataaaatga gcggtaacgg taaggttaca
    10981 gtaaggtagc taattttatt ccacgcgcat aatgctgaac ttcgagcgaa cttagagaat
    11041 agaaggttac tactcccaaa cacgaacaca tggtcgcatt tcggaatgct ggaaaatgtt
    11101 ggcacgtact gacataatac gtcaacctat ttcaatagtt gtaaaacgct cggtaaattc
    11161 acttaaagaa cttgatatat cggtcgttta ttaatttact ttttccgttg tgacagcttc
    11221 taaaatcagt tggcattttt agcgtatagt attcagaaaa tcggactttt cggctgtctt
    11281 aaagagcaaa atgcgagtcc ttaaaggtaa agcaaccaaa attagtggaa aatagtggaa
    11341 ttcggcgcgt gggtatcaga tagtcatgat ttagatgtaa aactcccaat aacttctccg
    11401 ataatacgga aatcactatc tctatctatg agtatatcgt catacttgtc gttcagacta
    11461 tgcagaaacg ctccagaatc gtttataaca agttgtttga tataagcatc accgtaatac
    11521 tcaaaaactc ctatatcacc atctgacagg ttaatagata gtttgataaa tacatagtcc
    11581 ccagaatggt attcgggttc catagaatcg ccataaaccg gtatgacaaa atcagcgtcg
    11641 taatcgactg gtaattcaat tgtttctact tgtacatcat ttagatactg acctgtacca
    11701 gctgaagctg cgtggtcgta gtagttgtaa gtgtagtagg tggcttgtgg ttcttctact
    11761 gtgttttgtt gttctaattg tgtttcagca taatcaagga cgtttaattg tcgcttgtgt
    11821 tctagctgag ataaagtaga tgttactttt tgtagggttg atgggataat attactattt
    11881 gtttcaatat tattagaatc aaaacggggg tcaatgtctg attttagaac tccgaagaag
    11941 tccgccagct tttgaacgtt tccagcagtt gggagtgaac gacctttaac atagccagtt
    12001 atcgtactct taggtattcc tagtttgttg tgaatatcta tctgtttgat attcttttta
    12061 gccataagca tgttgagatt tgttgagaaa atttctctat ttattaaatc ttggggacta
    12121 tttttagcca tttctcttac ccctttcttt ttgtatctga attaattcta acacaa
//
LOCUS       DATUYR010000046        11892 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.046, whole genome shotgun sequence.
ACCESSION   DATUYR010000046 DATUYR010000000
VERSION     DATUYR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.046"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..165)
                     /locus_tag="VCI33_001258"
     CDS             complement(<1..165)
                     /locus_tag="VCI33_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2832183.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVT"
     gene            complement(267..365)
                     /gene="ffs"
                     /locus_tag="VCI33_001259"
     ncRNA           complement(267..365)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VCI33_001259"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            565..1797
                     /locus_tag="VCI33_001260"
     CDS             565..1797
                     /locus_tag="VCI33_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEP2832184.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEQLCLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(2411..2926)
                     /locus_tag="VCI33_001261"
     CDS             complement(2411..2926)
                     /locus_tag="VCI33_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEP2832185.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(2926..3705)
                     /locus_tag="VCI33_001262"
     CDS             complement(2926..3705)
                     /locus_tag="VCI33_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP2832186.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDSFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGIDPDNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(3848..4384)
                     /locus_tag="VCI33_001263"
     CDS             complement(3848..4384)
                     /locus_tag="VCI33_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEP2832187.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTK"
     gene            complement(4388..4699)
                     /locus_tag="VCI33_001264"
     CDS             complement(4388..4699)
                     /locus_tag="VCI33_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832188.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(4916..6058)
                     /gene="tgt"
                     /locus_tag="VCI33_001265"
     CDS             complement(4916..6058)
                     /gene="tgt"
                     /locus_tag="VCI33_001265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEP2832189.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVIKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            6277..7140
                     /locus_tag="VCI33_001266"
     CDS             6277..7140
                     /locus_tag="VCI33_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEP2832190.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGVILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGGSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
     gene            7226..8568
                     /locus_tag="VCI33_001267"
     CDS             join(7226..7676,7676..8568)
                     /locus_tag="VCI33_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP2832191.1"
                     /translation="MKFNQETKVKIYELRQMGESIKSISKKFDMAESDLKYMIRLIDR
                     YGVTIVQKCKNNYYSPELKQEIINKVLIDGQSQKQTSLDYALLTSSMLSRWIAQYKKN
                     GYTILEKPRGGPSKMGRKRKKNLEEMTEVERLQKELEYPRAENAVLKKPERIPLEGRS
                     KAQRATEIIQALRNQFPLEMLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDE
                     HKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMGLAARIRRKRKYSSYKGEVGKKADN
                     LIKRHFEGSKPYEKCYTDVTELALPEGKLYLLPVLDGYNCEIIDFTLSRSPNLKQVQT
                     MLEKTFPADSYSGTILHSDQGWQYQHQSYHDFLESKGILPSMSRKGNSPDNGMMDSFF
                     GILKSEMFYGLEKSYKTLDKLEEAITDYIFYYNNKRIKAKLKGLSPVQYRTKSFQ"
     gene            complement(8868..9740)
                     /locus_tag="VCI33_001268"
     CDS             complement(8868..9740)
                     /locus_tag="VCI33_001268"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEP2832192.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLASGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            9964..11274
                     /locus_tag="VCI33_001269"
     CDS             9964..11274
                     /locus_tag="VCI33_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEP2832193.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAICCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISTQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            11352..11645
                     /locus_tag="VCI33_001270"
     CDS             11352..11645
                     /locus_tag="VCI33_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP2832194.1"
                     /translation="MPRKTFDKAFKLSAVKLILEEEQSVKMVSSTLEIHPNSLYRWIQ
                     EYEKYGESAFPGHGSALRHAQFETKKLEKEHKLLQEELALLKKFQVFLKPNRK"
     gene            11678..>11892
                     /locus_tag="VCI33_001271"
     CDS             11678..>11892
                     /locus_tag="VCI33_001271"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP2832195.1"
                     /translation="MNSHHTCQTLQVSRSGFYAYLKRRLSSKHVENEALKEMIKDIFY
                     EHKERYGSVRITQELCRRGIHVNHKRVG"
BASE COUNT         3681 a         2494 c         2172 g         3545 t
ORIGIN      
        1 agtaacaaat gcaatttcta cattttcata atcgcatggg gtaatattat aggcaaacct
       61 taaacttctt tttaaatctt ttaaattttc atctgcataa gctaattgac ttccatagct
      121 aaaaacacat gataatatga taattgtcaa aatgtttgtt ttcatttaaa actttttatg
      181 tcctgtttta tacctcttaa cctaaataaa aatatcaatc ggtttcaaca actgatgaca
      241 ctatagaagg aggagattac tttagtaaag tcaacaaaaa aagagcacac acccgaaaat
      301 cgcttagggc tgctggattc ctcccctgac ccacttcacg cacaagtgtt gctccgccta
      361 ttattatatc atattacgga cagtttgcaa gttttccaac taagtaaaat cactttcaag
      421 tgagcatagc ttcattttgg gacaagatgg actgaatacc tagaaaaatc atgacccttt
      481 ccaaacacga ttttgctttt tgaaaatctt tccctctccc cattccagct attctctatg
      541 ttataataag attattagat tagaatggga gttatgatga aacaaaagat taaaatctta
      601 acagtaattg gccttatgac agttggtatg tctgcgtgtc acaacactag caaaccttct
      661 aataccgatt ctgttttttc tttaaccgga aaaaagcgcc aacaaatcgt caaacaagta
      721 agacaacggt attatttcca acaattatcc aaaaccgaac aagaaaatta cctcacttta
      781 tacgatagtc tagcacaatt tcgtgaaatc atcagtttga cgccggcttc taaaaaaagc
      841 ttaattaaaa cgattgacgc ctttgtgatg gataatcccg aattttactg gattacatct
      901 gccgattatc gatttgagtt ttctgatcaa acggtctttg tcacctttcc tatcccagaa
      961 gatgccaaga acgtttacca agacttgcaa gcaatcggta atgatatcgt cgcaaacacg
     1021 ccatcaaagg atcgctatga gcaggtcaaa tatttttatg aggtcatcat tcgagacaca
     1081 gactacaata aaaaagcctt tgaagcctat caatcaggca gtcaagccca ggtcgcttct
     1141 aatcaagaca ttaaaagtgt ttttattgat catttatctg tttgtaatgg ctatgcccaa
     1201 gcctttcagt ttctttgtca aaaagctggc attccagtcg cctatatccg tggaactggt
     1261 acatctcagc aacctcagca atcttttgca catgcatgga atgctgttca aattaataac
     1321 acttattatg gcgttgatgt cacttggggt gaccctgttt ttgataacca tctctcacat
     1381 caaaagcaag gaactatcaa ttacagtttt ctatgcctgc cagattatct aatggcctta
     1441 tcacatcagc caagcaaaga cattgctttt aatactaaag aacgttttga aaatgtttgg
     1501 actatacctt cttgtacaga tgattctttg ctttattcta aacgtcatca aagctatatc
     1561 tcaacgtttg acagtgacgc catccttgca agccttgaaa atcagttgtt aaatagacaa
     1621 gagcaactat gtctacaatt tgctcatcaa gatgattatc aacagatggt aactgattta
     1681 acgacgaatc aaacaggtta tcataacctt ttcaatcagt attggaataa ttatacgggc
     1741 tttacctatg gactcttacc agagactctt agtatttcat ttgctagtag gaactaggtt
     1801 tagacacatg aaacacaaag ttaagatgat tatctttgtc aaacattaca agaaattgat
     1861 atactgacac aattgaaggc gattattatt ctaaccgtct tcaatagata taaccttttc
     1921 agattgagca catctaatgc ttgtaacatt agcaacagtg atgagctctt ttttaatgct
     1981 ttagcaaaat atatcagcta agacaaaaga ttaaataact caagccatgc ctataagtca
     2041 cttgttttta atccctgtgt cagtgtcaat ctacgccaca gataactgac agctctgagc
     2101 cttagaagtc tgagctggat aagtttacta aaaacaaaaa taaggtgagc cattactatc
     2161 tgtttttaga tagtaaatct ttttaagaat gactaactca tctagtcatc acgtgtttta
     2221 tttttcgatt gttttcacct cgattgggtc tgaaaaagag cgcttctagt acttggaaac
     2281 gcccttttgt atattctatc ttcgtatgtt gaatctaaca accattggaa aaatggtcta
     2341 acgtttcact cattattttg acaggttacc cttcaccaaa aatgcatgtg ataacaagct
     2401 ccttaaaact ctaatcaaag ggatctgact gttccttgat caaatgctta gctatttttt
     2461 tgcgctctcg cccttggcgg aaaaaggttt gcatgatatt agcacagtct gctgctagca
     2521 ggcctcgttc aacttgtacg cggtggttta agcgctcatc tgtcaaaatc tggtaaaggc
     2581 tgtctgctcc accaaatttt tgattgctgg ctccataaat aacatgcgga atccttgcta
     2641 gcccaatggc accactacac atcacacaag gctcgatggt cacaaagagt gtggtgtcta
     2701 ataaacgcca gttgccttca tgagcgttag cctcgttaat ggccatcatt tcagcgtgca
     2761 taatggcttg gttactctct tcacgcgcat tgtgcccacg accaataatc tcaccatcct
     2821 tgacaatgac acagccaatg gggatttcag ctttctgtaa ggatttttcg gcttctttta
     2881 aagcctcttg catgaaataa gtttgctctt ctaaactata tggcatcaag aaagactccc
     2941 caagttccaa gatttgtgtg gtaaggcaag gtcaacagga atattacctg cataggcttg
     3001 tgtttcttgc ctcaacactt ccaagtgatt gtcaggatca ataccttctg gaggaaccgg
     3061 tggcatgtgg gtcaagacaa gcttcttaac acctgcctgg ctagccatta gtcctgcttc
     3121 cttactggtc aaatgggcca tatgattttc attgccttcg tataagtaaa catctgctaa
     3181 gaaaaggtct gcttcttttg cgaagtctgc caatccttca aaataaccac tgtctccagt
     3241 aaagacaagt acctgaccag tcgctctttc tacaattcga aaggcataac acacaactgg
     3301 atgaactgtt ttaagaaagg taatgtcaaa aggaccaatc tgttcaactc cagtgacatc
     3361 ataggctcta ccttctgaaa catcaggaat gcttaactta gcaaattcgt gtcggtcctc
     3421 atcatgccca taaatgggca atactttagg ctgccacagg tgtttaggat aaagttgaaa
     3481 ataatggcgc aacacgccta aatcggccac gtggtctgga tggtaatggc taataatcac
     3541 tgcatccaaa tcgagaggac tgatttcttt ttccagctca ttgagcgctc gactaccagc
     3601 atccatcaat aactgaaagc tgtcatgtcc tgtgaccaaa taagaggttg tgcctgcatc
     3661 ttgataggga taggctcccc aagaccctaa ggtggttaat ttcatctcaa acccttcttt
     3721 cttgctttaa ctcttattat aacaaaaatt agtgtcaaag aggattgaga cccaccaaaa
     3781 acaggtccta gcagataaat ctgccaagac ctgttcaaca caacttaact atttgatgat
     3841 tagattacta ttttgtattc aaaaaactat ctttgtattt aacagcgata atggtcgcaa
     3901 tgattgcttt gatggtatct cctggaataa agaccaagtt tgacaaaagc gatgtcacaa
     3961 gagacatgcc agtgtaggct gccaaccaga tagcacccag aacatcaacc agtacaactc
     4021 ctgagattaa aagagctagg aatgccaatg gcgtgctttt tgagaaccag ttgcggtaaa
     4081 gtaatgaaaa gacaataggc acaagaaggt aggcaataac atagccagca gatggcccca
     4141 tcaatactgg aatggttgtt cgtgagcctg agaaaacagg taagaaaagg ccaatcacta
     4201 aaaataagaa aacagataag gttccctttt taccacccag catcaatcca gccaacatga
     4261 cacctaagtt ttgtaaaaca atagggactg ggataaagcc tagcgggata gcaggaataa
     4321 agcctaaaat aataatcaaa gtggtcatca tagccacctt aacaagttct ttggttgtaa
     4381 acatctgtta cgactccttt aaaaaataga tatccttgtg cctatgacag gctggattaa
     4441 agggactttg acaataagga cagcatccac aaccatactc agctaggctc aagagctttc
     4501 gacaatgccc acaaataaca ggagatgttt cttgatgtcc tgttggcaca aaggcatgat
     4561 ctgttaactg gtcatggcaa tggtagcaag cataataagt ctgacaaaaa gcacatttga
     4621 gcccaacaat atcaagcggg gtatgataat gtaggcaccg atactcttgg tcaaggtcaa
     4681 ttccaaaaca gtccgtcata tctcatcctc ttgtaacagt ttaatcaaaa gcatatgatt
     4741 tgtcaactct atttttacaa aagggtaact gttttctgaa aaagaacacg taaaacaaga
     4801 acttcccttt aaagagggac ctatcagttg aaagcaatag gacatccgtc tatacaaaaa
     4861 aactgaaacc aaagacgata gatcgccctc agttccagga tcatagcatt tttaattaga
     4921 aattacggtt tgatttatta taaccatacc gttcaagaaa atcttggcgg aattcaagta
     4981 agttatcgtc cataatagcc tgacgtactt ttttcatcaa gttaaccaag aaataaaggt
     5041 tgtggtagct tgtcaaacga ataccaaaag tttcgtccgc ttttagcaag tgacgaatgt
     5101 aggcacggct atagttttga caagtgtagc agtcacagtc atgatcaagt ggggtaaaat
     5161 cttcagcgaa tttggcattt ttaatcacaa gacgcccctc actagtcatg caagttccgt
     5221 tacgggcgat acgcgttggc aagacacagt caaacatatc tacaccacga atcacaccat
     5281 caatcaagct atctggtgct cccactccca tcaggtaacg aggtttattt tctggtaaga
     5341 ggggagtcgt aaagtcaagg acggcattca tttcttcatg gctctcccca acagctaaac
     5401 caccaataga ataacctggg aaatccatag ccaccaaatc tgccgcagac tgacgacgaa
     5461 ggtcctcaaa tccagctcct tgtacaattc caaataatcc ttggtcatga ggacgacgat
     5521 gggcttttaa gccacgttct gcccaacggc tagtacgttc aatggatttt ttcacgtaat
     5581 cataaggttg gtaaaattgc gggcactcgt caaaactcat catgatgtct gagccaaggt
     5641 tattctgaat ggaaatggct ttttcgggtg atagaaacat ttttgagcca ttcaagtgat
     5701 ttttgaaagt cactccttct tcagtgatgt ttcgtgaatc ggctaaagag taaacttgaa
     5761 aaccacccga atcggttaag attggctgat cccagttcat aaacttgtgc agaccaccag
     5821 agcgagcaat caactcatct cctggacgca accaaaggtg ataagtgttt gataaaataa
     5881 tccctgatcc aattgctttt aactcttctg gagactgtgt tttaacagta gcctgagttc
     5941 ccactggcat aaacattggt gtcggaaagg tcccgtgagg ggtgataatt tcgcccaagc
     6001 gtgcaccggt gtgcttttct gttttgataa ggcgatactt aatcggataa tctgtcatgt
     6061 gctttcttcc ttgctatcaa gataaacagt cttgagcttt tttgatctgc ctgagtttcc
     6121 atcataatga agggcttcaa gatgacatgt tgttctagcg cctatcattt tatcaaaaaa
     6181 tccataattt gtcatgcttt cccaaaaaag tatagtcaaa ataatggtta gctgtctcat
     6241 tttgttttat ggtataatga aataaaggag tctgttatgt caattaaagc aatcaaaggc
     6301 caggctagag acacgttaaa aaatctctct ggcaagtatt tactattttt aattccaacc
     6361 cttctcttta tgtttcattt cggtattgag attcatcaag gttacgtcct atctagtggt
     6421 attgaagtct cgctagcggc tagttatttc ccattactac taggagttat ccttagcctt
     6481 ttcattttat cagcatcctt tacaatgatt gatgttgtta ggcattttcg tcagaaagtt
     6541 tcttttgccg aatcaacaac agccttctca aaagaattct ttgggaacct gctggtttta
     6601 gcgatcacta agtggctatt ctttctcatc tggtcactaa tttggttttt tggcctcttt
     6661 attttccttt ccggactttc agcgtttttg gtcaatgcta aatcaggtgg ctcaacagtc
     6721 atttcactga tctttttact atttggagca gttttgtcct taattggttt tggtatttat
     6781 atcaaccgtt attacgctta tagtctatca gaataccttc tctacgataa ggttaaagag
     6841 gggacttatc taggagctat agccgtcatt gaaacaagtg tagctatgat gaaaggttat
     6901 aaatggaagc tttttttctt acaacttagc ttcaccggtt ggtttctact gaatattgtt
     6961 acttttgggt tacttaatat ctatctttta ccatatttta caacagccaa tgtcattttt
     7021 tatgatcaac tcaagaaacg tttcaaagac aaagatgacc caattgaagg agaacattta
     7081 agccttcatc agcttaataa aaagcctact cctatgatgt atgacgatca aaaagactaa
     7141 ataaatcctc catccataaa aatagccctt gaactgcacc ccaaaagtta gacacaaaat
     7201 ctaacgattg gggtgctatt tttttatgaa atttaatcaa gaaacgaaag ttaagattta
     7261 tgagttacgg caaatgggag agtccattaa atccatatca aaaaagtttg atatggcaga
     7321 atccgatctc aaatatatga ttcgcttgat tgataggtat ggggtaacca ttgttcaaaa
     7381 atgtaagaat aattattatt ctccagaact gaagcaagag ataataaata aagtcctgat
     7441 tgacggccaa tctcagaaac aaacatcctt agattatgct ttactaactt ctagtatgct
     7501 ctcaaggtgg atagcgcaat acaagaaaaa cggttatact attcttgaaa aaccaagagg
     7561 gggaccgagt aagatggggc gtaaacgtaa gaaaaatttg gaagagatga cagaggttga
     7621 acgtcttcag aaggaattag aataccctag agcggagaat gctgtgctaa aaaagctgag
     7681 agaataccgc ttgagggacg aagtaaagct caaagagcaa cagaaatcat tcaagcatta
     7741 aggaatcaat ttcccttaga gatgctactt gagatccttg atttatcacg ctccacctat
     7801 tactatcaag tgaagcgact agctcaagga gataaggata tagaactaaa gcatgtgatt
     7861 cgagagattt atgatgagca taaaggtaat tacggttatc gtcgtattca tatggagttg
     7921 cgtaaccgag gatttgtggt caatcataag aaagttcaac gtctcatgaa agtcatgggc
     7981 ctagcagccc gcattcgtcg gaagcgcaag tattcttctt acaaaggaga ggttggtaag
     8041 aaggctgata atctgattaa acgtcacttc gaaggctcta aaccttacga aaaatgttac
     8101 actgatgtga cggagttggc cttacctgaa ggaaaactct acttattacc tgttcttgac
     8161 ggctataact gtgagattat tgatttcact ctgtctcggt ctcctaactt gaaacaggtt
     8221 caaaccatgc ttgagaagac ttttccagcg gattcataca gcggaactat tcttcatagt
     8281 gaccaggggt ggcaatacca acaccagtcc taccatgatt ttttagagtc taaaggtatt
     8341 ctgccatcca tgtcccgcaa agggaatagc ccggataacg gcatgatgga ttccttcttt
     8401 ggcattctca aatctgaaat gttttatgga ctcgaaaagt cttataaaac ccttgacaag
     8461 cttgaagaag ctattacaga ttacattttt tactacaaca acaaacgaat caaagcaaaa
     8521 ctaaaaggac ttagtcctgt ccaatacaga actaaatcct ttcaataatt atttgtccaa
     8581 ctttttgggg tcagtacact cctagttaac gactttgatt gattttatta tgatgaatcg
     8641 tgatttctga aaacaaaatt ttcttcatgg atttggcgtg gaacttttac aataaaaaca
     8701 ctaacctctc cctatcgttt tttcaggtga atgcttggaa ttggtataat aagcggacac
     8761 gaatcaggtg aaaacagaga aagtatttga agaggcgttt aaactatctg acataaaact
     8821 cgttctagag gaaagacatc ctttaaaaag ttagtttatt ttgcaactta gacatcaagg
     8881 taggttaaac ccttcatgga aaaatcgaga ctcttagcac tatgggttaa actaccactg
     8941 gagacgtaat caatcgctaa accacgaaaa cggctaatag tggtcatatc aatatttcca
     9001 gaacattcaa tccgagaacg tcctgcaatt agagtaatgg cctgttcaat ctgttccaat
     9061 gacatattat ccaacatgat aatatcaaca cccgccgccg cagcttcttc ggcagcagca
     9121 agactttcca cttccacctc gaccattttc acaaaagggg cataggcacg cgcttgagca
     9181 atcgcctttt gaacactacc tactgccgca atgtgattgt cttttagcat gatagcatct
     9241 gataaattaa agcgatgatt atagccaccg ccaactctca cggcatattt ctcaaaaaga
     9301 cgtaaattag gagtggtttt tcgagtatca aataccttaa tgcgatcatc gcctaaggct
     9361 tctacataag cagctgtcat cgaagcaatc cctgataaat gttgtaaaaa attcaaggca
     9421 acgcgttcac atgttaagag gcttctcacc gagcctatga tttctaaaac caaatcgcca
     9481 ctagccaaac gatctccatc cttaaattga tgaggattct ggaaggtcac ctcggcatca
     9541 aatagggtaa aaaccctttg aaaaacggtt agccccgcta aaacaccagc ttccttggca
     9601 aaaagcgaca ccttggcttg gccatgatga tcaaaaatgg cattggtact gtaatcttcg
     9661 gaatgaacat cttctcgcaa ggctgctttc aatgtatcat ctatttgaaa aggggttaaa
     9721 tcagttgaaa tgattgacat cactaagtcc tctattttta atatcacttg taagcactag
     9781 aggcaattgc tattaacttc cgtaggaaat cacaaacctg aatacctcgt tttaaattcc
     9841 aacaagtgtc aagacaattg taattttttt gtgtataata gtcaagtacc aataggaaac
     9901 aggtggtctt ttgagcgcca aaattgccaa cctatttaca tattctaact aaaggacaat
     9961 tcaatgattc atttaataat aattagcgcc attgctctag ccattggaat tggttaccgc
    10021 accaaaatca atattggcct actggctatt gctttttctt acctcatcgc aaccactctc
    10081 atgggattaa gccccaaaga acttcttcat ttttggccaa cctcactctt ttttaccatt
    10141 tttagcgtct ctctctttta taacgttgca acaactaacg gcactcttga tgttttggct
    10201 caacacattc tctaccgcac acgcacccac cctaacgccc tctacatgat tttatacctg
    10261 atagcaaccc ttttgtctgc tttaggtgct ggatttttca ctactatggc catttgctgt
    10321 cctctagcga ttaccctctg tcaaaaagcg gacaaacacc ctttgattgg agctcaagcc
    10381 gtcaattggg gagcttcagg aggggctaat ttgataacca gtggctcagg cattgtcttt
    10441 caaggcctgt ttaagcaaat gggatgggaa gagcaagctt tttcacttgg caatcatatc
    10501 tttatcgtca gcattatcta cccactcatc gtcttgcttt tattatcttg ttacatccgc
    10561 tacagtaagg gaagaacaaa ctcatccttg actattgacc aaccaccagt cctcagcaag
    10621 gtccaaagac aaaccacctt gctcatgatc agcagcatgg ttttggtttg gctttttccc
    10681 ttgttgcatc tcattttccc aaacatcgca tggatagcta cttatcgaca aacatttgat
    10741 attggctttg tctcaatact catggtctgc ctcgccttaa ggctcaaatt agggaagcaa
    10801 gaagccattt tggctaaggt tccctgggct ataattatca tgctctgcgg catgagctta
    10861 ctaatgtctc tagcggtcaa gtcaggttta gttacactga tagggcacct cataacaaca
    10921 accattcctc atttctggtt acctctgttc ttttgtgtga tagccggagt gatgtcactc
    10981 tttagttcca ccctatccgt tgtagcccca actctctttc ccatcattgc taccatcagt
    11041 actcaaagtc ctcacattga tattcgctta ctcacaacag ccactattat tggcgctcta
    11101 tctactaata tctctccttt ttcatcagct ggttccctca ttcaactctc ccttccccat
    11161 atagaggaac gcagtcttgc tttcaaaaaa caaattcttc tgggtgtgcc catcagctta
    11221 agtttggcac tcttgaccat ctggattctc atgctgctag cttccttaag ttagatttat
    11281 tgtctaacat ttggttggac ttggtatact aaagtagaca caaaattaag tggaaacgag
    11341 gaatagtcat tatgccaaga aaaacctttg ataaagcctt caaactctct gctgtaaaac
    11401 tcatccttga ggaagagcag tctgtaaaaa tggttagttc aactttagaa attcatccca
    11461 atagtcttta tcgatggatt caagaatatg aaaaatatgg agaaagtgcg ttcccaggac
    11521 atgggagcgc acttcgtcat gctcaatttg agacaaaaaa acttgaaaaa gagcacaaac
    11581 tgctacagga ggaattagct cttctaaaaa agttccaggt ctttttgaag ccaaaccgga
    11641 aataaaattt cagtttctga aggaaaatag tgttagcttg aatagtcatc acacctgtca
    11701 aactcttcaa gtctcccgtt caggtttcta tgcttattta aaacgtcgtc tatcttcaaa
    11761 gcatgttgag aatgaagctt tgaaggaaat gattaaagac attttctatg aacataaaga
    11821 gcgttacgga agtgtacgaa ttacccaaga actttgtaga cgtggcatcc atgtcaatca
    11881 taaacgtgtt gg
//
LOCUS       DATUYR010000047        10203 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.047, whole genome shotgun sequence.
ACCESSION   DATUYR010000047 DATUYR010000000
VERSION     DATUYR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.047"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            108..296
                     /locus_tag="VCI33_001272"
     CDS             108..296
                     /locus_tag="VCI33_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2832196.1"
                     /translation="MLITENIAVKVRKKRAVKQLGKVELSKKLGITPPTLSKIEAGNY
                     KAPKRIYASVMEWLAEDY"
     gene            312..878
                     /locus_tag="VCI33_001273"
     CDS             312..878
                     /locus_tag="VCI33_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEP2832197.1"
                     /translation="MNRQVSIFEEVNDQGGAFQVVTTMTFHDRLLDIYGNIQEPLFRA
                     RDVAEMIDHSKTRNGYYDVQSMLRKVDDDEKVKGVPLEGATKNFRSGQKVWFLTEHGL
                     YEVLMRSSKPKAKEFRKAVKNILKEIRLNGYYMQGELVQNSQPTPDIDDLSYIKQKLT
                     DLQDVDSLQDLRWKMAKLYHVIETLDEY"
     gene            935..1177
                     /locus_tag="VCI33_001274"
     CDS             935..1177
                     /locus_tag="VCI33_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HEP2832198.1"
                     /translation="MKATTYKELKKWIDEGVDLAELAQGYADKVPSVDREQFEAVTQG
                     IFNVLEGVSLMLDDKVLIYNRKAEQKRLNDIEQGDY"
     gene            1398..1580
                     /locus_tag="VCI33_001275"
     CDS             1398..1580
                     /locus_tag="VCI33_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832199.1"
                     /translation="MTSVRTAQLLNNGLGLVGQDLKDVLSALANSVNPSIEKQISHIM
                     IDRANTGEWLANVGKK"
     gene            1592..1924
                     /locus_tag="VCI33_001276"
     CDS             1592..1924
                     /locus_tag="VCI33_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832200.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKNKAEAYFTRLYNQH
                     IAWVIIANVMTEYVNKFRKSATSFEKAWDALGYQQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            1924..2115
                     /locus_tag="VCI33_001277"
     CDS             1924..2115
                     /locus_tag="VCI33_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832201.1"
                     /translation="MQELNLTPTQTLILFIVLGLVGLLLSLSKPLIEIDLPEDTQVPK
                     PPQNANYGAYIQSQNHYYN"
     gene            2024..2392
                     /locus_tag="VCI33_001278"
     CDS             2024..2392
                     /locus_tag="VCI33_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832202.1"
                     /translation="MTYQKIPKYLNHLRTQTMGLIFNHRTIITIRREQMQLLSREAEL
                     ELLEKVGCYLEKRWQLELENKDDWDLIPRQDLLEKLDITGTTLKNWEKEGLKSYQSPF
                     ENSKKVYYRKSDIYNFLSVD"
     gene            2402..2923
                     /locus_tag="VCI33_001279"
     CDS             2402..2923
                     /locus_tag="VCI33_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832203.1"
                     /translation="MDYLEVTMTKDCVKIFNFLYKYPTKAYQKDEYFKLLYMHPLDSF
                     LTSFSLSGIRVKVTDKPVLAGWKLVRDIEVRIATGELLEMIEELEICYLRKHQTVSYV
                     EIKFYVVHLLTYGIRSRYDMQFFTKLLFCCGYDQETVIGIYSNITKNTRLSRDFITLQ
                     AKLYQTKKGTHEH"
     gene            2913..4526
                     /locus_tag="VCI33_001280"
     CDS             2913..4526
                     /locus_tag="VCI33_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEP2832204.1"
                     /translation="MSIKENIDKLVSMEWTESIFGQSKMKADYKRQLSKVIELDTLSN
                     IHHNGETYAVSNQEVMYLSESKGRTHKFISGNVKTKSKGKGRDSQKYLVVSFDHQFPP
                     FAKLVVDYLLGRFTFFNNKLYDVNNRQAQVLDDFTIQSLYGFKKDNPFILEILEGIHQ
                     TLNIRPAKQLEAYKISGRDFIIDLKEHRLYRTNPSSDSSFFKFYDVDYHTAIGSKKKV
                     GKFLDYVIADHDSLHNATLQAYYIAQVACGLKSKQNFFISKSGVRTGKGLRHIGLSGI
                     FNKIDVELDLLIKGGFEASNAWGAFAGGEMALATEQGDIVGDKMERVLKIIATEKTHM
                     ARSIGGNLGTVNLTSVLCIDTNRTVALSDEMNGRAVLIQYQNRPEGETDLEREKAFEE
                     YWQTFTEMDKSPKIEGVIGFLLVSLDYFRKNDMKFDWRAVEVVNDDDLDDFQVYLINH
                     LSEKDFVERDSYVRELYNSTYGKNNNQAGQRMRTIGVKSYRKQTTGGRKIPGYKVDNI
                     KRFNKFSGGEKENTNIFDMFEKLNQPLPP"
     gene            4813..4986
                     /locus_tag="VCI33_001281"
     CDS             4813..4986
                     /locus_tag="VCI33_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEP2832205.1"
                     /translation="MKIKLFYQKHNESLDDFEYRVNQFTLSVSVIDIKLSEATYGNYE
                     DMSTTTSLLVLYR"
     gene            4992..5165
                     /locus_tag="VCI33_001282"
     CDS             4992..5165
                     /locus_tag="VCI33_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832206.1"
                     /translation="MKLKLHTRGGNTITIQGDSAFYDELVKYLLSGRQPNWVTCPSAV
                     INLSDIIAITKEK"
     gene            5167..5493
                     /locus_tag="VCI33_001283"
                     /pseudo
     CDS             5167..5493
                     /locus_tag="VCI33_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5489..5734
                     /locus_tag="VCI33_001284"
     CDS             5489..5734
                     /locus_tag="VCI33_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832207.1"
                     /translation="MSNQMAVFNQFWAYQTLFQPTAQDIKLAILGYFTGTLWACEVSV
                     LEDVELGYRVIVSYVSCFDKSDRLLKIAQAVENSQLG"
     gene            5748..5906
                     /locus_tag="VCI33_001285"
     CDS             5748..5906
                     /locus_tag="VCI33_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832208.1"
                     /translation="MQTEVKDGFIYFKENGRIGVVEIPTFGKLTLSSKEGYIHQVDHS
                     FSTKHNQK"
     gene            5997..6785
                     /locus_tag="VCI33_001286"
     CDS             5997..6785
                     /locus_tag="VCI33_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832209.1"
                     /translation="MYKQTNKTYADILEKVEAATAQWIIRKKASSLKDDVIITDEDIQ
                     AEKERIFIEKYGTALFDFKIDSYRKELDDLKIKAREQVNQFNDFVKELEQDDRYTERG
                     KVDLYRAERKKAEKTLKEIGEAQHKLNVDIQKIEVESAQNAWKKLESEMTPDSITPSE
                     YQYLEMMLSRNDSDEMRKKLAEKFHYHLMVLEYLNADRKFGEVIIKHPLEGVKNQSVN
                     YIKIGDVRLPEVYESTYIHELLKHYKNDIYFGESYANHEWDEEQ"
     gene            6782..7048
                     /locus_tag="VCI33_001287"
     CDS             6782..7048
                     /locus_tag="VCI33_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832210.1"
                     /translation="MMSLECLRIYLKKSQFTELEHLLFRIIVLGGYPDDMYFPSRVRT
                     IITSLVNNIRKNLDSEGYRSVEELEEAIEKAISEHDEITQKGGG"
     gene            7090..7428
                     /locus_tag="VCI33_001288"
     CDS             7090..7428
                     /locus_tag="VCI33_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEP2832211.1"
                     /translation="MVAMVTSKTITEAAERAGITRGTAHRYLKDITFKKACRRYRSEL
                     MQQTTSLLQSASIEAVEVLRNIMLDDSISPYARQQSAQTILSTAYKAHELDDIVEMVE
                     ELEARFEDEQ"
     gene            7418..7693
                     /locus_tag="VCI33_001289"
     CDS             7418..7693
                     /locus_tag="VCI33_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832212.1"
                     /translation="MNSNLKKRVDKLRRLHSLDNASGIVFVEYADGKYQIKKQDHVPK
                     FIETRLIAEFESEEEAENFLSTIPSGYIIFWDIFPEEVEHVKKNKYL"
     gene            7668..7913
                     /locus_tag="VCI33_001290"
     CDS             7668..7913
                     /locus_tag="VCI33_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832213.1"
                     /translation="MLKKINTYRRLEIIKNKLDEASKDSIEDIMLDWFRALHYALKVD
                     DTIDLPTFLEFKDNKALRDKIMADLGDFDPFEDLNIE"
     gene            8194..8541
                     /locus_tag="VCI33_001291"
     CDS             8194..8541
                     /locus_tag="VCI33_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP2832214.1"
                     /translation="MNAKEQLKELKPLFALMTLFEEQRDKDIKLMNAFRNPELLNGIE
                     KGTAKQLLYLAKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEIPDKLGYSRNTVF
                     KLHREALEVLDEP"
     gene            8531..8902
                     /locus_tag="VCI33_001292"
     CDS             8531..8902
                     /locus_tag="VCI33_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP2832215.1"
                     /translation="MNHKNAIRKLKEFHRWQRIANSLNLTYNECYQFDIEYYSFRRKH
                     LEISRKCALEELDAIKHAINQLSKIEYRKILIECYLIGEKKPQQDIIAELNRSKSWYY
                     ETKRRALLEFVELYRDGVLKK"
     gene            9055..9282
                     /locus_tag="VCI33_001293"
     CDS             9055..9282
                     /locus_tag="VCI33_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation repressor RelB"
                     /protein_id="HEP2832216.1"
                     /translation="MAMVSLRLNSAEEELFRSYATHTGKTLSELFKTALAQQIEDQLD
                     YETGIQALKRFEENPITYSIDDLIEELENDL"
     gene            9272..9541
                     /locus_tag="VCI33_001294"
     CDS             9272..9541
                     /locus_tag="VCI33_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP2832217.1"
                     /translation="MTYKLVVSDEVKKQLKKMDKHVGLMLAKDMKKRLDGLNNPRQFG
                     KALTGQYKGLWRYRVGNYRVICDIVDNEMIILALEVGHRKEIYKR"
     gene            complement(9915..>10203)
                     /locus_tag="VCI33_001295"
     CDS             complement(9915..>10203)
                     /locus_tag="VCI33_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEP2832218.1"
                     /translation="EVFEFLQREQLLSSLLSANGTKEIQAFIINKVRLLITTDLQDKF
                     STEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKKESPQEMTQFVLKMLTST"
BASE COUNT         3626 a         1647 c         2072 g         2858 t
ORIGIN      
        1 acgaaaaaaa attggatttt tatcttgaca accaaattta tttgcgttaa tatagatttt
       61 gcaaaaataa gacgaaaaaa aattgggttt ttgcaaagga ggaatatatg cttattacag
      121 aaaatatagc tgttaaggtt cgtaaaaaaa gagctgttaa acagttagga aaagtggagc
      181 tttctaaaaa attagggata acgccaccca ctctatcaaa aattgaagct ggcaactaca
      241 aagcacctaa gcgtatctac gctagtgtca tggaatggct agcagaggat tattagaaag
      301 gcaggaacaa aatgaataga caggtcagta tttttgaaga agtcaatgat caagggggag
      361 ctttccaagt tgtaaccact atgacttttc acgatcggct attagatatt tacggtaata
      421 ttcaagaacc gttatttaga gctagagatg ttgcggaaat gattgatcac agcaaaacaa
      481 ggaacggata ttatgacgtt caatctatgc taagaaaggt agatgatgac gaaaaagtta
      541 aaggcgtccc tttagagggt gctacgaaaa atttccgtag cggtcaaaaa gtttggtttt
      601 taactgagca tggactttat gaagtgctta tgcggtcaag caaaccaaaa gccaaagagt
      661 ttagaaaagc agtcaaaaac attctcaaag aaatccgttt gaatggctac tacatgcaag
      721 gagagcttgt ccaaaacagt caaccgaccc cagacattga tgatttaagc tatatcaagc
      781 agaagttaac tgacttacag gacgttgaca gcttacaaga tttacggtgg aagatggcta
      841 agttatatca cgtcatcgaa acactggatg aatattaaga aataagacaa caaaaaaaag
      901 cgcaataata cgcacggata aaggagaaat gattatgaaa gcaacaactt acaaagagtt
      961 gaagaaatgg attgatgaag gtgttgattt agctgagcta gcacagggtt acgctgacaa
     1021 agtacctagt gtagaccgtg aacagtttga agcagtcaca cagggaattt tcaacgtatt
     1081 ggaaggcgta tcgctcatgc ttgatgacaa agtgctaatc tataatcgca aagcagagca
     1141 aaagcgtttg aatgacattg aacaaggtga ttattaatta ataatcaaga gtaacaaaaa
     1201 aagccttgaa ctttggcgag gaacaggctt ttcacttcaa ttaactaaaa ccataaaaaa
     1261 gcaagtatgg gatttagtta ggtatttatt taattatacc acaatcaaca tcagggagct
     1321 accccttaaa actagcatga atctagtata agaaacaagc aactacacag aaaataagaa
     1381 ttaataagga agataaaatg acatcagtta gaaccgcaca attacttaac aatggtttag
     1441 gattggttgg tcaagaccta aaagacgtct tatcagctct agccaatagc gtcaatccaa
     1501 gtattgaaaa acagatttct cacatcatga tagatagagc taatactggc gaatggttgg
     1561 caaatgtagg caaaaaatga ggtaagacat aatgaaatat agagtagaaa caaatccttt
     1621 ttcaaaagat agatacactc ctgaacagct agaaatgttc aaaaatcgac aactcagcaa
     1681 aaataaagca gaagcctatt tcactcgact atataaccaa catattgctt gggtgattat
     1741 tgctaacgtt atgacagaat acgtcaataa attcagaaaa agtgccacca gctttgaaaa
     1801 agcatgggac gctttaggct atcaacaaac cacagagatt gtctttagag ccgttaacgg
     1861 tttaccttgt tcagagaaag acacagggga actagaaact tatttaagtg aggtatcggc
     1921 atgatgcaag aacttaacct cacaccaaca cagacactta ttttattcat tgttttaggg
     1981 cttgttgggc ttcttcttag cctttctaag ccattaatag agattgactt accagaagat
     2041 acccaagtac ctaaaccacc tcagaacgca aactatgggg cttatattca atcacagaac
     2101 cattattaca attaggaggg aacagatgca attattatca cgagaagcag aattagagct
     2161 actagagaag gtgggctgtt accttgaaaa acgttggcaa cttgaactag aaaacaaaga
     2221 tgattgggat ttaattccaa ggcaagatct actggaaaaa ctagacatta ctggtaccac
     2281 gttaaaaaat tgggaaaaag agggcttaaa gtcgtatcag tcgccattcg agaacagtaa
     2341 gaaggtgtat taccgtaaaa gtgatattta taattttttg agcgttgact aggaggactt
     2401 tgtggattat ttagaggtca ccatgacaaa agattgcgta aaaatattca attttttgta
     2461 taaatatcct accaaagcat accaaaagga cgagtatttc aaattactgt atatgcatcc
     2521 cttggacagc tttttaacaa gtttctccct atcgggcatc cgagtgaaag ttacagacaa
     2581 acctgtttta gcagggtgga aacttgttag ggatatagag gttagaattg ctacggggga
     2641 actcttggaa atgattgaag agctagaaat atgctattta aggaagcacc agacggtttc
     2701 ttacgttgaa attaagttct atgtcgtcca tctattgact tatggtatta ggtcacgtta
     2761 cgatatgcaa ttttttacca agttgttgtt ttgttgtggt tatgatcaag aaactgttat
     2821 cggtatttat agcaatataa ccaaaaatac cagactttca agggatttta taactttaca
     2881 ggctaaactt taccaaacaa agaaaggaac ccatgagcat taaagaaaac attgacaaat
     2941 tagtatctat ggaatggact gaatcaattt ttgggcaatc taaaatgaaa gcagactata
     3001 aacgccaact atctaaggtg attgagttgg ataccttaag caatatacac cataatgggg
     3061 aaacctatgc tgtttcaaat caagaagtca tgtacttaag tgagagtaaa ggtagaactc
     3121 ataagtttat ttcaggaaac gttaaaacaa aatctaaagg aaagggaaga gatagccaaa
     3181 agtatctcgt ggtttccttt gaccatcaat tcccaccttt tgctaagtta gtggttgatt
     3241 atctacttgg acgatttact ttttttaata acaaattata tgatgttaac aatcgtcaag
     3301 cgcaagtgtt agatgatttc actattcaat cgttatacgg cttcaaaaag gataacccgt
     3361 tcattttaga gattctggaa ggaatccacc aaaccttgaa tattagacct gcgaaacaac
     3421 ttgaagctta taagatttct gggagagatt tcattattga tcttaaggaa catcggttat
     3481 accgaacaaa tcccagttcc gatagttctt tctttaagtt ttatgatgtc gattatcaca
     3541 cagcgatcgg tagtaagaaa aaagttggca agtttttaga ttatgttatc gcagaccatg
     3601 atagcttgca caatgcaacg ttacaagcct attatatcgc acaggtcgct tgtggactga
     3661 aatcgaaaca aaatttcttt atctctaaat ctggtgtgag aactggtaaa ggcttaagac
     3721 atattgggct tagcggtatc tttaataaaa tcgatgttga gttagactta ttgattaaag
     3781 gtggctttga agctagcaat gcttggggtg cttttgctgg tggagaaatg gctctagcaa
     3841 cagaacaagg ggatattgta ggcgataaaa tggagcgtgt gcttaaaatt attgctactg
     3901 agaagaccca tatggctaga agtatcggtg gcaatttagg aacagttaat ttaacaagtg
     3961 ttctgtgtat tgacacaaac cgcactgttg cgctgtcgga tgaaatgaat ggtagggcgg
     4021 ttttgatcca ataccagaat agaccagaag gggaaactga tttagaacgt gaaaaggcct
     4081 ttgaagaata ttggcaaacc ttcactgaaa tggataaaag ccctaaaatt gaaggagtga
     4141 taggtttttt acttgtatca cttgattact tccgaaaaaa tgacatgaaa tttgattgga
     4201 gagcggtcga agtggtgaac gatgatgatt tagatgattt tcaagtttac ctgattaacc
     4261 atctttctga aaaagacttt gtagagcgtg actcttatgt tagagagctg tataactcta
     4321 cttacgggaa aaataacaac caagccggcc aaagaatgcg gacgataggg gttaagagtt
     4381 atcgcaagca aacaactgga ggacgcaaaa tacccggcta taaagtcgat aatataaaac
     4441 gatttaacaa attctcagga ggagaaaaag aaaatactaa tatctttgat atgtttgaaa
     4501 agctaaacca accactaccg ccatgaccca gacagtaggg gttgtggggg gtataaaccc
     4561 agtggtatca agggatagag tacaatcacc cataaccacc tataactata taggggagag
     4621 agaattattt ttttagtaga tatgttattt ctttacatta tttttatttt ttctccctta
     4681 tctatataga aaagtggcgg taggtggtgg ttgtgctggc aaatggctca accatgcggt
     4741 tttaagcaca ccaccacttt atttagggtt gtgtctacac ccaccacttt gagccaaaac
     4801 aggagaaaaa acatgaaaat caaactattt tatcaaaaac acaatgaatc tttagatgat
     4861 tttgaatatc gggtcaatca atttacccta tcagtatctg taatagatat caaactttca
     4921 gaagcaactt atggcaatta tgaagacatg agtaccacaa cttctttact ggtcttgtac
     4981 aggtaactga tatgaaacta aaattacaca cacgaggcgg caacaccatc accatacaag
     5041 gagacagtgc cttttatgat gagttggtca aataccttct ttctggccga caaccaaact
     5101 gggtaacatg tccttctgcc gtcatcaatt tatcagacat tatagcaatc acaaaggaga
     5161 aataatatga gaacattttc agacacacca aaaacattca catttcacta cacgtttaaa
     5221 gactttgaca ccgcacaagt agcctgtcac gccattctag gctatatgac tggtacttat
     5281 gagcaaccag tgattgatgc cacctatcac aatgataatc aaggcgggca tgctaatcag
     5341 ttagtattag aatatgccga ggacagaaag ttaagcaagg tcttcaagcg tatctgtgac
     5401 agcttcaagg attattacaa ccaacctgag gatatgacgg atgaagaact tgatgacatg
     5461 gttcaagaaa atgaattaat caaggaagtt gagcaaccag atggcagtat ttaatcaatt
     5521 ttgggcgtat cagacacttt ttcaaccaac ggcacaagat attaagttag ctattttagg
     5581 atacttcaca ggcacgctat gggcttgtga ggtgtccgtg ttggaagatg ttgaacttgg
     5641 ctaccgtgtt atcgtctctt acgttagttg ttttgataaa agcgatagac tgctgaaaat
     5701 tgctcaagca gttgagaata gtcagttagg atgaaaggag gtatttaatg cagacagaag
     5761 tgaaagatgg ctttatttat ttcaaagaaa atggtagaat aggagtagta gagataccta
     5821 cttttggtaa gctaacccta tctagcaagg aaggctatat tcatcaagta gaccacagct
     5881 tttctactaa acacaatcaa aaataattat actgaccgaa gaacggaggt atattcatga
     5941 agcttaatgg cttttttgga tatacctctt tttgcattta agaaaggaaa gacacgatgt
     6001 acaaacaaac taacaaaact tacgccgata ttttggaaaa agttgaagcc gcaacagcgc
     6061 aatggattat tagaaaaaaa gcttcaagcc ttaaagatga cgtgattatc actgatgagg
     6121 atatacaagc tgagaaagag cgtattttca ttgagaaata tgggacagca ctgtttgatt
     6181 ttaaaattga tagctaccgt aaagaattag atgatttaaa aatcaaagct cgtgagcaag
     6241 taaaccaatt caatgatttt gtaaaggaac tagaacaaga cgaccgttac acagaacgtg
     6301 gtaaagtgga tttataccga gcagagcgaa aaaaagctga aaagaccctt aaagagattg
     6361 gagaagctca gcataaactt aatgttgata ttcaaaagat tgaagtagaa tcggctcaaa
     6421 atgcttggaa aaagttagaa tctgaaatga cgcccgattc aatcacccct agtgaatatc
     6481 agtatttaga aatgatgtta tcacgtaatg atagtgatga aatgcggaaa aaacttgctg
     6541 agaagttcca ttaccatctg atggtcttgg agtatttgaa tgctgaccgt aaatttggag
     6601 aagttatcat caagcaccca ttagaagggg ttaaaaatca gtcagttaat tatattaaaa
     6661 ttggggatgt ccgattgcct gaggtttacg aaagcactta tattcatgaa cttttgaagc
     6721 attacaagaa tgacatttac tttggcgaga gctacgcaaa tcatgaatgg gatgaagagc
     6781 aatgatgtca ttggaatgtt taagaatata ccttaagaaa agccagttta cagagcttga
     6841 acacttgcta ttcagaatta tagtgttagg ggggtatcct gatgacatgt atttcccctc
     6901 aagggttcgt acgatcatca caagcttggt taataatata cgtaagaatt tagattctga
     6961 gggttaccgt agtgtcgaag aattggaaga agctatagaa aaggctattt cagagcatga
     7021 cgaaatcaca caaaaaggag gggggtgatt tttatagaac atggtttaac acctaaacaa
     7081 gagaaattca tggtagcaat ggtgaccagt aaaactatca ccgaggcagc cgaaagagca
     7141 ggtatcacaa gaggaactgc ccatagatac cttaaagaca ttactttcaa aaaggcttgt
     7201 cgccgttatc gtagtgagtt aatgcagcaa accacatcct tattacagag tgccagcatt
     7261 gaagccgttg aggtgctacg taacattatg ttggatgatt ctattagtcc atatgcaagg
     7321 caacaatcag cccaaactat cttaagcact gcttataaag cgcatgaact agatgatatt
     7381 gttgaaatgg ttgaggaact agaagcgaga tttgaagatg aacagtaact taaaaaagcg
     7441 agttgataag ttaaggcgac tacatagttt agataacgct tctggtattg tctttgtaga
     7501 atacgcagat ggcaagtatc agattaaaaa acaagatcat gtccctaaat tcattgagac
     7561 tagacttata gcagaatttg aaagcgaaga agaagcagaa aactttttaa gcactattcc
     7621 aagtggttac atcatttttt gggatatttt ccccgaagag gttgaacatg ttaaaaaaaa
     7681 taaatactta tagaaggctc gaaatcatca agaataagct cgatgaagcg agtaaagata
     7741 gtattgaaga tataatgctg gactggttta gggctttgca ttatgctctt aaagtagatg
     7801 atacaattga tttaccaacg ttccttgaat ttaaggacaa caaggcattg agagataaaa
     7861 taatggcaga ccttggagat tttgacccat ttgaagatct aaatatagaa tagttaaatg
     7921 gtgacagcat cgccacaagg tcgtcataag gagatagttt tttgactgtc tccctttttc
     7981 gtcatctatt ccccatcaga ctaaaataaa acatggtttt agcgcttctg tctgctaagt
     8041 tgatgatttt atattataag ctctgaaaag cgcttagaaa cgattttaga gccgttcttc
     8101 cgctatcaag aatgactaaa acaaaaagac gtccacacgg aacgccttca tgattatatc
     8161 tgttaacaca attataccac actgggggct ttcatgaacg ccaaagaaca acttaaagaa
     8221 ttgaaaccac tttttgcttt aatgacctta tttgaggaac aacgagacaa ggacatcaag
     8281 ctaatgaatg cttttcgtaa tcctgagtta ctaaatggca tcgaaaaagg tacagctaaa
     8341 caacttttat atttagctaa ggaacgtgat aagagactag ccatgattgc cacattgcaa
     8401 gatgagagac agattgccgt tatcaaagct agatacgtgg atgacttatc atgggatgag
     8461 atacctgata aactaggtta ttcaaggaat accgttttta aattacatag agaagcttta
     8521 gaggtattag atgaaccata agaacgctat tcgtaaacta aaagagtttc atagatggca
     8581 acgtatcgct aatagcctta atttaaccta taacgagtgt taccagtttg atatagaata
     8641 ttattccttt agacgtaaac accttgaaat aagccgaaaa tgcgccctag aggagctaga
     8701 tgctattaag catgccatta atcaactatc taagatagag tatagaaaga tactgattga
     8761 gtgttacttg atcggtgaga aaaaacctca acaagacatc atagcagaac ttaacagaag
     8821 taaaagctgg tattatgaga ctaagagacg tgctttactt gaatttgtgg aattatacag
     8881 agacggtgtg ctaaaaaaat aatgttcgtt tatgataaca gagcaataga tataaatgtt
     8941 aataatacag tgttagaaga gcttaacaag gtatatcaat acgggtaaat aattgactga
     9001 ttcctcctta tgttatacaa tgtattacag ataaacaaac agaggtggaa gaatatggca
     9061 atggtatcat taagactgaa cagcgcagag gaagaacttt tcagaagcta cgctacacac
     9121 acaggcaaaa cattatctga attattcaag acagcactag cacaacaaat tgaagatcaa
     9181 ttggactatg aaacaggtat tcaagcttta aaacgctttg aagaaaatcc cataacttat
     9241 tctatcgatg atttaattga ggagttggaa aatgacttat aagctagtgg tgagtgatga
     9301 agtcaagaag caactcaaaa agatggataa acatgtaggg cttatgttag ccaaagacat
     9361 gaagaaacga cttgacgggt taaataatcc tagacaattt ggaaaagccc tcacaggtca
     9421 atataaagga ctttggcgct atcgtgttgg taattatcgg gtgatttgtg acattgtgga
     9481 taatgaaatg attatccttg ctttggaagt tggacacagg aaagagatat acaagcgcta
     9541 gcaaatacaa gacatggtac gaggtataat aaaaagcgca gctaatgacc acgctatttc
     9601 ttgcttactg aactcattat tttaattcct ttttagttac cctttagatt tctgatagtg
     9661 tatctagttt acactgtttt tagagaaata agagtgataa ctcacttggt ttacagctac
     9721 tttacactac taaatatgag tagtataaca aaatgcttta attttaagat taaacttaca
     9781 aaaagcctat ttaataggct ttttttgtat ctcataacaa atttatcctg atcccttcat
     9841 tttactatgg cttctaaaaa agttatcact aaggctgatg acttgtaaaa tgatactttc
     9901 gtaagggtta ttatttaggt actcgtaagc attttgagaa cgaattgtgt catttcttga
     9961 ggtgattctt ttttaccttt agcaatccaa ctttgacaga caccaaaaaa agcatgggct
    10021 aagtagatac tttggtattc tttttccgtt tgagataact cttcggtact gaacttgtcc
    10081 tgtaaatcag tagtgatgag taggcgaact ttattgatga taaaggcctg tatttcttta
    10141 gtaccgttag cagacaaaag ggaagagaga agctgttctc tttggagaaa ttcaaatact
    10201 tca
//
LOCUS       DATUYR010000048        10096 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.048, whole genome shotgun sequence.
ACCESSION   DATUYR010000048 DATUYR010000000
VERSION     DATUYR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.048"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..299)
                     /locus_tag="VCI33_001296"
     CDS             complement(<1..299)
                     /locus_tag="VCI33_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2832219.1"
                     /translation="MLRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEFDFKIRKF
                     LMESKEIHLTKSPYIRGSLEIHSKNRKHEKINLYDAKPNSTRSDVLKKYKDNKTI"
     gene            complement(340..1365)
                     /locus_tag="VCI33_001297"
     CDS             complement(340..1365)
                     /locus_tag="VCI33_001297"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEP2832220.1"
                     /translation="MRKRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIIYSEVT
                     PYHVTSVWTKGVIPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWFDQNKEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKA
                     FPGLSARRIGVMPDLALDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGD
                     QSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTCANVRINHVINLWGADFDSN
                     GNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQ
                     DSWNQTN"
     gene            1869..2071
                     /locus_tag="VCI33_001298"
                     /pseudo
     CDS             1869..2071
                     /locus_tag="VCI33_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            2273..2653
                     /locus_tag="VCI33_001299"
     CDS             2273..2653
                     /locus_tag="VCI33_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEP2832221.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            2653..3501
                     /locus_tag="VCI33_001300"
     CDS             2653..3501
                     /locus_tag="VCI33_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP2832222.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            3626..4834
                     /locus_tag="VCI33_001301"
     CDS             3626..4834
                     /locus_tag="VCI33_001301"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEP2832223.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            4831..6708
                     /locus_tag="VCI33_001302"
     CDS             4831..6708
                     /locus_tag="VCI33_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP2832224.1"
                     /translation="MSHFLVKKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDATNLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGSYSDYLDEKAFEKETVEADLAKAIVTEEVPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(6806..6883)
                     /locus_tag="VCI33_001303"
                     /pseudo
     CDS             complement(6806..6883)
                     /locus_tag="VCI33_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7600..8010
                     /locus_tag="VCI33_001304"
     CDS             7600..8010
                     /locus_tag="VCI33_001304"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP2832225.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLSLTGLTAQICQHELDHLEGILI"
     gene            complement(8093..10072)
                     /locus_tag="VCI33_001305"
     CDS             complement(8093..10072)
                     /locus_tag="VCI33_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEP2832226.1"
                     /translation="MSILTSFTLLVTDVQADQVDVQFLGVNDFHGALDNTGTAYTPSG
                     KIPNAGTTAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANSALLQDEPTVKV
                     FNKMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEHEASHQTIVIAN
                     VIDKKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQNYEHYQFLDAA
                     ETIAKYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQIYPEHSIDIIFA
                     GHNHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFMTTPSANVIAVAPGIKTE
                     NSDIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNLVTTAQLTIAKK
                     TFPTVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQMTGQHIYDVLNQ
                     QYDENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLTTTYTVVVNDFL
                     YGGGDGFSAFKKAKLIGAINTDTEAFITYITNLEASGKTVNATIKGVKNYVTSNLESS
                     TKVNSAGKHSIISKVFRNRDGNIVSSEIISDLLTSTENTNNSLGKKETTTNKNTISSS
                     TLPITGDNYKMSPIMTILALISLGGLNAFIKKRKS"
BASE COUNT         3187 a         1651 c         2005 g         3253 t
ORIGIN      
        1 tttatagtct tattgtcttt atattttttt aagacatcag atctggtgct attgggtttt
       61 gcgtcatata aattgatttt ttcatgtttc ctatttttac tgtgaatttc taaactcccc
      121 cttatataag gagacttagt caaatgaatt tcttttgatt ccatcaaaaa ttttcttatt
      181 ttaaaatcga actcttgaat cgtaacaata tccttatcga tttttatagg atttattaat
      241 gttttttgct gttgcccatc tataaaaaaa ttccctgcaa tagtattagt ccttagaata
      301 ccatcatctg ttgaatgtaa tctttcttat tgatacgctt taattggtct gattccaact
      361 atcttgccct gttgaaagtg taaataaccc tagtacttga gcaccaatat tatcttcttt
      421 tatttcttta gcagaaatag ctacttttcc agcggagtta acaccaacaa agtatttctt
      481 cataccaata gatgcattac tatcagagtc tgttacataa atagctttaa ggttcccgtt
      541 agaatcaaag tcagctcccc acaggtttat aacatggttg atgcgtacgt tagcgcaggt
      601 gtgtgatagg cctagagcct tgccttcggt taactctttc ttaatgagat cactgatttc
      661 tttgagattt ttttctttaa aatcatgacg acttgtcaat agcttacttt gatcacctct
      721 tgtaaatacg gcgtcaaaaa taccacctcg gcgatctttc tcttgatagg ttctattgac
      781 atcagtagtc tgtgtcttat aaacatttaa gtaataacca ttgataaaca tatctaaagc
      841 aagatcaggc ataactccaa ttcggcgtgc tgacaaaccg gggaaagctt tatctcggaa
      901 ataattaaaa agctcgctat ttgtttggtc acctttagta ttaataactt ttcttacatc
      961 caataattct tgatcaccaa acatgatttt ttgtttatct gggtgttttt ttagatatgc
     1021 ttcaattttt tctttgtttt gatcgaacca ccagtgaagc atattccctg ctgtggcagc
     1081 cccgcaaaga agatcgtctt ttccattgaa tgttttggta atatcatacc atccttggtt
     1141 agcaacataa ggagcgtgaa aaacatcttc gccttgagtg aagtttgctg gaggaataac
     1201 tcctttggtc caaacggaag taacgtgata aggtgttact tccgaatata taatctcttg
     1261 attagcagaa aaactatctg ctataacacc acgatctacc gatagagcaa ataaagtcac
     1321 tgctgccaat actgcagctg aagttgaata gcatcttttt ctcataagaa tacctttttc
     1381 tatataattt tattataaaa agattataac atatagacat ctattgttgc aattttttaa
     1441 aataatactt aattttttaa ataattttta ataaacgtaa acaagcgatt gacaatggag
     1501 atatcacaag agatacagta gtggttgtgc ttaaaatgga cggttttttg atgatatgtt
     1561 gccgcatgag gaagtgagca ataggagagc tgtgatagaa gaaagagagg aagaagcgtt
     1621 gatgaaatta gacaaataaa aaaaaagacc tcatccagaa gaggtcgtgg tataactggg
     1681 gacgataggt tcaattgata aaaaatcttg ttagcttacg atctatgttg ttttgaatgc
     1741 ctttaactca acatcgttaa tggattttga gataaaaaaa taaatccaat agtctggtac
     1801 tttcctattt tttgtctgtt aaaacgtttt tattttataa tggataaaag gagaagaagg
     1861 gcggagcgat gattaagctc tatgggttta agtatgggtt agttggagag gttttgcggc
     1921 ggattgaacg cctaggattt acgtttgagc gattagagct aagacaagct agttcgaaat
     1981 acttggctaa gcatgatgag gccttggtga taaacctttt tatcctggac ttaaaactta
     2041 cctgaccagt ggttctgttt taattgaggt gcagtacaag accactaacg ggtgtttttt
     2101 tgatataatg tcagtataag gacgactaaa tataaatagg tgaaaaaata gatatctgtt
     2161 cgagagatag cactgtttaa aacgttatgc gaaaatagta gtttaggggc tactttaata
     2221 atcttcaaat ctatgctata atggatcata ttgataccaa ggagaataaa aaatgcaatt
     2281 agttcgtgaa aaagaatttg tcaaccagta ccattatgat gctagaaatt tagagtggga
     2341 aaaagaaaac ggaacaccag agaccaattt tgaagtgact tttcagttga ttgataaaga
     2401 tgagcagcaa aaagaaacag taattgtatc tgtattgcaa tttgttattg tcaaagaaga
     2461 atttgttatt agtggggtca tctcacaaat ggttcgtatt ttagatcgtt tagtggataa
     2521 accaagcgaa tttacgcaag aggaagttga atccttagct gctcctttgc tagacatggt
     2581 taagcgattg acttatgaag tcacagaaat tgccttggat cgtccaggta ttcatttgga
     2641 gtttaaaaat taatgaaatt agcagtaatt actgacagta cagcaacact accaacagat
     2701 ctgaaacaag ataaagctat ttttagcctt gatataccag ttatcattga cgatgagacc
     2761 tattttgaag gtcgaaattt gagtatagat gatttttatc aaaagatggc tgactcccaa
     2821 aatcttccca agacaagtca gccaagttta tctgaactag ataatctttt aggacttttg
     2881 tcttctaagg gctatacaca tgttattggc ttgttcttag ctggaggaat ctcaggcttt
     2941 tggcaaaata ttcaattttt agctgaagaa catcctgaga ttgaaatggc atttccagac
     3001 agtaagatca catctgctcc tttaggtagt atggtaaaaa atgtgctaga ttggtcacgt
     3061 caaggaatga catttcaagc tattttaaac aagctgcaag aacagattga tgggacgaca
     3121 gcctttatta tggttgatga ccttaatcat ctggttaaag gcggacgcct gtcaaatggg
     3181 tctgctcttc ttggaaatct tttgagtatt aaacctatct tacgctttga tgaagaggga
     3241 aaaatcgttg tttatgaaaa agtgagaaca gaaaagaaag ccatgaaacg cttggttgag
     3301 attttaaatg acttgatagc agacggtcag tataatgtgt ctattattca ctcaaaagca
     3361 caagacaaag ctgattattt gaaacgactc ttacaagaca gtggttatca atatgacatt
     3421 gaagaggttc attttggagc ggtgattgct actcatttag gagaaggagc cattgcattt
     3481 ggcgtaacac cgaggcttta attgtcgatt agaaaaaata gttaattgca agaatcacta
     3541 aaacaataac aaaatgtgat agaacccaag tagcctaatc acactttaga ttgggctact
     3601 tggatgatag gttagaaaac aacgtatgaa attaatgaca atgccttctg agtttcagaa
     3661 ggctttaccc atattgacta aaataaaaga agctggttat gaagcctact ttgtgggagg
     3721 aagcgtcaga gatgttttac tcgagcgtcc cattcatgat gtagatattg caactagttc
     3781 ctatcctgaa gagactaaag cgatttttaa cagaactgtc gatgttggca ttgaacatgg
     3841 tacagtgctc gtgcttgaaa atggcggtga gtatgaaatc acaaccttta ggacagaaga
     3901 tgtctatgtt gactatcgcc gtcctagtca ggtttctttt gtgcgctctt tagaggaaga
     3961 cttaaaacga agagacttta ccgtcaatgc cttggccttg gatgaaaatg gtcaagttat
     4021 tgataaattt agaggactca ttgatttgga gcaaaaacgg ttacgagcag tgggtaaagc
     4081 agaggaacgt tttgaagaag atgcactacg cattatgaga ggctttcgat ttgcggcgag
     4141 tctggatttt gacattgaag ctgcaacttt tgaagctatg aggagccatt cacctctatt
     4201 ggaaaagatt tctgtggaac gttcttttac tgaatttgat aaattattga tggcaccgca
     4261 ctggcgaaaa gggatttctg ccatgattgc ttgtcaggct tatgactatt taccaggctt
     4321 gaagcagcag gaagcaggct tgaatcatct gatagtttct ttgaaagata actttacctt
     4381 tagtgatcac caccaagctt gggcttatgt gatgattagt ttagccattg aggatcctaa
     4441 aagcttccta aaagcatgga agacttcaaa tgattttcag cgttatgtca caaagttgat
     4501 tgctctttat agaattcgtc aggaaagatc ttttgaaaaa ttggatattt accagtacgg
     4561 caaggaaatg gcaagtctag tggaaggcct ccgaaaagct caaagcttat ctgttgacat
     4621 ggaccacatt gaagctttag atcaagcgct agccattcat aacaaatacg atatcgtcct
     4681 taatggttct cacctgatta aggattttgg gatgaaacca ggtcctcagc tagggcttat
     4741 gcttgagaaa gttgaattag cgattgtaga gggacgccta gataatgatt tcactacaat
     4801 tgaagcattt gttagagagg aattagcaac atgagccatt ttttggtgaa gaaattgaca
     4861 aaaacggtgg gagataaaac agtctttcag gatatttcct ttattattca tgactttgac
     4921 cgtattggga ttattggggt taatggaaca ggaaaaacaa ctcttttaga tgtcttgtcg
     4981 ggaaggcttg gttttgacgg agaccattca ccattttcta aagccaatga ttacaaaatc
     5041 gcttacttaa cacaagatcc tgaatttaac gatgcatcct ctgttttaga tacggtattg
     5101 tcatctgatg ttaaggcgat tcagctgatt cgtcagtatg aactattgat ggcgaattat
     5161 actgaagata aacaagagac cttagagtcc ctcatgtctg aaatggatcg cttagatgct
     5221 tggtctattg aaagtgatgt caaaacagtc ctatcaaaat taggcatcac ggatttggaa
     5281 caaaaagtgg gagacctatc aggtggtatg agaagacgtg tccagttagc tcaagtattg
     5341 ctaggagctg ctgacttact cttgttggat gagccgacca accatctcga tattgacacc
     5401 atcgcttggt taacaaccta tctcaagaca gctaaaaaaa ccgtcctttt tattacccat
     5461 gaccgttatt tccttgatca tgtggctacc cgtatttttg aattggataa agcgggactg
     5521 acagaatatc aaggcaatta ccaagactat gtgcgcttaa aagcagaaca agacgaacga
     5581 gatgctacta atttacacaa gaagaagcaa ctctataaac aagaattagc ttggatgagg
     5641 acgcagccac aggcgcgtgc aaccaaacag caggctagaa tcaatcgttt cactgacttg
     5701 aaaaaagaag ttcaccaaga cagttctgca gataaattgg agatggcttt cgaaacgagc
     5761 cgaattggga agaaggtgat tcactttgaa gacctttctt ttgcttatgg cgaccgacag
     5821 ttgattaagg attttaactt gattattcaa aataaagatc gtattggaat tgttggagat
     5881 aacggcgtcg gaaaatctac cctgttaaat atcatcaacg gtgatctcaa accaacttct
     5941 gggaagcttg atattggaga tactattcgt gtaggttatt tctctcagca gctcaaagat
     6001 atggatgaga ccaaacgggt cattaattac cttcaagaag tggctgatga ggttaaaaca
     6061 agcgtgggaa caacgagtat cagtgagctt ttagagcaat ttctttttcc gagatcaagt
     6121 catggcacgc tgattgctaa attatctggt ggggagaaaa aacggctcta cttgctaaaa
     6181 ctgctcattg aaaagccaaa tgttcttctc cttgatgagc caacaaatga tttagacatt
     6241 gccaccttga cggtcttaga aaacttttta gctaactttg caggaccagt cattacggtt
     6301 agtcacgatc gttatttcct agacaaagta gccactaaga ttttagcctt tgaagaaggc
     6361 gatattcgta ttttttatgg gagttacagt gattaccttg atgaaaaagc ttttgaaaaa
     6421 gaaacagttg aggctgactt agctaaagca atagtcactg aagaagtgcc acttcctccg
     6481 aaagaggaga aaaagcgcat gtcctacctt gaaaaacaag aatgggctca gatagaagat
     6541 aaaatagcaa ctattgaggc taatattgaa gaaattgaaa accaaatgtt aactgtagtt
     6601 agtgattatg gccagttagc tcaattacaa aaggaactcg atcagcgcaa taatgattta
     6661 ttattggcgt atgaacggtt tgagtatttg agtggactag acatataagt atactatggt
     6721 aatgttatgt ttataataaa caagtgataa tagaaaagaa gtgctcttgc actgagcccc
     6781 aaaagttggc tactctattt gaagtaaagg atttagttct gtattgtaga ggattaagtc
     6841 cttttcgttt tattttgata cgtttattgt tgcaataatc gatgataata gtaggtagaa
     6901 cgagtcaatt tagccgttct taaaagaaaa tctaatcaga actcgtcttt gtccattctt
     6961 tataatggtt ttttgtcttc ttctttgcag agatgcttct ttcaaacgga gttccctcgg
     7021 tttttttagc gctaaatatc tagcttatct tcatagctta atgtcataca aaaacacccc
     7081 aaacgttaga tttttttatc taacgtttgg ggtgtagttt acattatggc gcttcttttc
     7141 tattattacg tgaggaaaaa tcaagtaaaa aagttaagtc aactgaaaaa cttgacagaa
     7201 atattcagaa aatttataat actaactatt aacaacatat cagctattaa agcatttcaa
     7261 acagcaggtg ttttatttgg acctgttaag gctgcaaatg caagagaaag cagtatctgg
     7321 tttgaaaatc ttacaaagca ataactatct ctcttagaca tttgaagaag cagacagtaa
     7381 attcgataat attgtgatga gaacttagca acaatctgct gatgcaggtc aatttttatt
     7441 tggtactcat attgcagaat ttcataaagt tgcccaagat atcttgaaat aaggaatcac
     7501 ctgatgtttt aaacagatga tactaagtct atcatatggt gataatagtc cattgaacat
     7561 gtagtttatg aattaaaaag aaggtttaag gagataagaa tgattagaga aatcataaca
     7621 gatcattttt tattacagca aaaagcgcaa gtcgccaaga aagaagatct atggattggc
     7681 caagatttac aagatacctt agccttttat agacaggaat gtcttgggat ggctgccaat
     7741 atgattggtg aacaaaagcg cattgttatt gtgagtatgg gatttattga tttggttatg
     7801 tttaatcctg tgatggtttc taaaaaggga atttaccaaa ctaaagaatc ttgtttgtcg
     7861 ttatctggct atcgcaaaac acagcggtat gataagatta ctgttgagta tttggaccat
     7921 aattggcgac caaagcgatt aagcttaaca ggcttaactg ctcaaatatg ccagcacgaa
     7981 ttagatcatc tagaaggtat tttaatttaa caaaaaacct agagtaggac gctttaggtt
     8041 ttttttggca aagatgcctg attatataag ggtgatcgat tactagtaaa cgctaggatt
     8101 tccttttttt aataaaagcg tttagtccac ctaagcttat caaggcaagg attgtcataa
     8161 taggagacat tttataattg tcccctgtta ttggaagagt ggaactagag atagtatttt
     8221 tgtttgttgt tgtttctttt ttgccaaggc tgttattagt gttttcagta gaagtcaaaa
     8281 ggtctgaaat gatctcacta gacactatat tgccatcacg atttctaaaa accttactaa
     8341 tgatactgtg tttaccagca ctattaactt ttgtcgaact ttcaaggttt gaagttacat
     8401 aatttttaac cccttttata gtagcattaa cagttttacc tgatgcttct aaatttgtga
     8461 tatatgtgat gaaagcttca gtatctgtgt taatagctcc aattaattta gcttttttaa
     8521 atgctgaaaa gccatcacca ccaccataaa gaaagtcgtt gacaacaacg gtgtaagtag
     8581 ttgttaagtt aatttcttca ccattgtctt tataaacctt aactatcttg aagggggtat
     8641 cagagttctt aggatcatta tctgtataag tgtatgttaa acctgacatt tgaagaaaat
     8701 aggtctggtt ttcatcgtat tgctgattta ggacatcgta aatgtgttga ccagtcattt
     8761 gaatgacttg aaggatatta ccaaatggtt gtacagcctg tgcagctccc caggtgatgg
     8821 tccgatcatt tttgacaact aggtcacttc gaataccacc attattggtc atagcaaagt
     8881 caacagttgg aaaagttttc ttagcaatag taagctgagc tgttgttact aagttaccga
     8941 caggagattc tttatcaata ttttctgttt tagaaatagt tgaagaatta gttgcagttc
     9001 cgatttttcg ttcagtaact gttttaacaa tatcattagc atgatttatt atagctttga
     9061 tatctgaatt ttctgttttg atacctggtg ctacagcaat aacatttgct gatggagttg
     9121 tcataaaatc attggtatca gtatctagcg taccacggac atctgcataa gcttttcctt
     9181 gagagagagc ttgaacgata cgtgttttac cgatagttcc attagtgtat tgatgattat
     9241 gtcctgcaaa aataatatca atgctatgtt cgggatagat ttggttcact ttttccataa
     9301 ccgtagccat ttcatgatca acaacaccat ctttacttgt tgcaggaata tgagctaaaa
     9361 ccacaatagc atgaacatgt tgttcttgta gttctttagc atatttggca atggtttcag
     9421 ctgcatctaa aaattgatag tgttcatagt tttgctttaa aacgagattt ggaatctctg
     9481 tagtcacaac accaatgaag ccaatcttaa cgattttgtc attaatggct atgtctttta
     9541 tagcataagg tttccaacca taggggatat cattggtttt tttatcaata acattagcaa
     9601 tgacgatggt ttgatgcgaa gcttcgtgct catattgttt ggtgatatca ttaattgttg
     9661 attcaggatc aggcgcttga cctgtcatga tacggttaaa ttcatctagt ccttcgtcaa
     9721 attcatgatt accaagagtg ccatattcaa atttcatttt gttaaagact tttacagtag
     9781 gctcatcttg taaaagtgca gagttagcag gacttgctcc gaccatatct ccagcttgaa
     9841 cacgtatact tgttccgtct tgatttgctt gcttgaagtc tatctcagcg tcatccatat
     9901 aagcacctaa ttgagcagtc gtcccagcat ttggtatttt accacttggt gtgtaagctg
     9961 ttccggtatt atcaagagcg ccgtgaaaat cattgacgcc aaggaattgc acatcaactt
    10021 gatctgcttg aacgtctgta actaatagag taaaactagt caggatactc aatacactac
    10081 tttttaaaat aaaata
//
LOCUS       DATUYR010000049        10068 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.049, whole genome shotgun sequence.
ACCESSION   DATUYR010000049 DATUYR010000000
VERSION     DATUYR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.049"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            247..564
                     /locus_tag="VCI33_001306"
     CDS             247..564
                     /locus_tag="VCI33_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEP2832227.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRTKVNQLAFVVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
     gene            688..2586
                     /gene="mnmG"
                     /locus_tag="VCI33_001307"
     CDS             688..2586
                     /gene="mnmG"
                     /locus_tag="VCI33_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEP2832228.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDAEYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            2676..4652
                     /locus_tag="VCI33_001308"
     CDS             2676..4652
                     /locus_tag="VCI33_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEP2832229.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYHLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            4649..5101
                     /gene="rplI"
                     /locus_tag="VCI33_001309"
     CDS             4649..5101
                     /gene="rplI"
                     /locus_tag="VCI33_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEP2832230.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLTITEA"
     gene            5131..6498
                     /gene="dnaB"
                     /locus_tag="VCI33_001310"
     CDS             5131..6498
                     /gene="dnaB"
                     /locus_tag="VCI33_001310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEP2832231.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            6515..6787
                     /locus_tag="VCI33_001311"
     CDS             6515..6787
                     /locus_tag="VCI33_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEP2832232.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            7517..8128
                     /gene="rpsD"
                     /locus_tag="VCI33_001312"
     CDS             7517..8128
                     /gene="rpsD"
                     /locus_tag="VCI33_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEP2832233.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(8285..9448)
                     /locus_tag="VCI33_001313"
     CDS             complement(8285..9448)
                     /locus_tag="VCI33_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP2832234.1"
                     /translation="MNIKEYKKKNGTIIYRTSVYLGVDQVTGKKARTTITASTKKGVK
                     IKARDALNNFAMNGYTVKEKPTVTTYKELTALWWESYKNTIKPNSRQSMEGIVRLHIL
                     PAFGDCKLSKLTTPVIQQQVNKWANNANKGIKGAYANYSFLNNINRRILQYGVTMQVI
                     EHNPARDVIIPRKQNNKEHKVKFFSNQELKQFLNYLDDLDLSSYENFFDYVLYKTLLA
                     TGCRIGEVLALEWSDIDLKKGTIKVSKTLNRYQETNTPKSKAGLRDIEIDRATVLLLK
                     QYKNRQQVLSWDLGRSETIVFTPFTTKYAYACLLRKRLQKHFKAAGVPDISFHGFRHT
                     HATIMLYAGIEAKDLQYRLGHSNISMTLNTYVHATKEGAKKAVSIFETAISNL"
     gene            complement(9628..10050)
                     /locus_tag="VCI33_001314"
     CDS             complement(9628..10050)
                     /locus_tag="VCI33_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2832235.1"
                     /translation="MRKEQTNLNQKDFFDQVVNKKLNLDISFRTYQNWENPKNTIKPD
                     KAQILADYFGVWLPYLLGNSNIRTEEQANFLAEMNKRINSEGDSFSFDMTATESDEHD
                     LLYNYRQLETPYKNQVRKFTRDMWELQKESKNFSPKSP"
BASE COUNT         3194 a         1618 c         2074 g         3182 t
ORIGIN      
        1 aaaaacattg acattactgt aaaggtctgt gatgaaatca atattgacaa tcactaatca
       61 tttttttaaa atagataata acaatcggaa tgatggtcaa agctcatgaa agttgtaggt
      121 ttttttgtcc tgcaatcttc tagggttttg gcccttttta tggtttagct ctagaattga
      181 gatggctaat aggactggga tttagaatca ctagaggtca gataacttta gtttacgagc
      241 tagtgcttga ttgttagaaa tggtaagaat tttttaacta gagatgcagc tgaccaatat
      301 tacaccattg gtggtacaag tttggtaggt gaaaaaacgc atgaaactgt tttacgtgaa
      361 accttagagg aagttggtat tcgaactaag gttaatcaac tggcttttgt ggttgagaat
      421 cattttgata tcgatgatgt tttctggcat aatattgaat ttcattattt ggtaagcccc
      481 ttagaagacc ctaaactaga aatgatagag aatgcaagcg acaggtttgt gaatggattc
      541 caatcaatca actggatcaa atgaatcttg ttcctgagtt tttacaaacc gagttggcta
      601 aatggccagg acatattgtc cgcattgaag gctaaactaa agcgtatgat tttaaatgaa
      661 tagactgaaa taaaggattt taaaaaaatg acacatgaat ttacagaaag ctatgacgtc
      721 attgtcatag gtgcaggtca tgctggtgtt gaagccagtt tggccactag tcgtatgggt
      781 tgtaaaactt tattggcaac aatcaacctt gatatgttgg cttttatgcc gtgtaatcct
      841 tctattggag gatctgccaa agggatagtg gttagagaga ttgatgctct tggcggcgaa
      901 atgggtaaaa atattgataa aacctatatc cagatgaaaa tgcttaatac aggtaaggga
      961 ccagctgtac gcgctctccg agctcaagca gataaatccc tgtatgcgcg tgaaatgaaa
     1021 catacggttg aaaaacaggc taatttgact cttcgccaaa cgatgattga tgatattcta
     1081 gttgaagatg gacgagttgt aggtgtttta acagccacag gacaaaaatt tgcagctaag
     1141 gcagttgtag tgacaacagg aacagctctg cgtggcgaaa ttattttagg agaactcaaa
     1201 tattcttctg ggcctaataa tagtttggca tcagtaactt tagcagataa tttaaaaaaa
     1261 ctcggcttag aaattggcag attcaaaaca ggaacgcctc ctcgtgtcaa agcatcttct
     1321 attaattacg atcaaacgga aattcagcca ggtgatgaca aacctaatca tttttcattt
     1381 atgtcaaaag atgctgagta tctcaaagat caaattccct gctggttgac ttatacgaat
     1441 caaactagtc atgatatcat taatcagaac ctttacagag caccgatgtt ttctggcatt
     1501 gttaaaggtg taggccctcg ttactgccca tctattgaag ataagattgt tcgctttgct
     1561 gataaagaac gtcatcaact tttcttagag ccagaggggc gtgatacaga agaagtgtat
     1621 gttcaagggt tgtcaacgag tcttccagaa gatgtccaaa aagatttgat tcattcgatc
     1681 aaaggacttg aaaaggctga aatgatgcgc actggttatg ctattgaata tgatattgtt
     1741 ttacctcacc agctacgtgc cacacttgaa accaagctaa tttcaggact ctttacggct
     1801 gggcaaacta atggaacttc tgggtatgaa gaggctgctg gccaaggact tattgctgga
     1861 atcaatgctg ctttgaaagt tcaaggtaag ccagaactta ttttgaaacg gagcgatgct
     1921 tatattggcg ttatgattga cgatttggtt accaaaggaa ccttggaacc ttatcgattg
     1981 ttgacatcgc gagctgagta tcgtttgatt ttacgtcatg acaacgctga tatgcgtttg
     2041 acagagattg gtcgcgacat tgggttggtt gatgatgagc gttggaaggc cttcgaaatt
     2101 aagaaaaacc aatttgataa cgaattaaaa cgtctgaaca gtatcaaatt aaaaccagta
     2161 aaagcaacca acgatcgtgt gcaggaactt ggttttaaac cattgacgga tgctatgaca
     2221 gctaaagaat ttatgcgtcg cccagaaatt gattatgcga ctgcagtctc ttttgttggt
     2281 cctgcggctg aagatttaga tgctaaaatc attgaacttt tagaaacaga aatcaagtac
     2341 gaaggctata ttcgcaaagc tttagatcag gttgctaaga tgaaacgcat ggaagaaaaa
     2401 cgtattcctg caaatattga ttgggatgct attgattcta ttgcaactga ggcgcgtcag
     2461 aaatttaaga aaattaatcc tgaaacaatt ggacaagcta gtcgtatttc aggagtcaat
     2521 cctgcggaca tcagtatctt gatgatttat ttagaaggaa acggaaaagc tcatcgaaaa
     2581 tattaaatgg aaatgaaatt gtaatctcaa agcaagtgga agctctgtat ttcagggctt
     2641 ttttatggta taatgtgtgc ttaagaggtt ataagatgaa aaaatttcgt tttgaaacca
     2701 ttcatttgat tatgatggga ttgattttat ttggactact ggcactttgt gtcagtatta
     2761 tgcaatcaaa aatattaata ttattagcta tttttctcgt tttgctattt gttgttgctc
     2821 ttttatggta tcaaaaagaa gcttatcact tatcagactt agctcatatt gagctcttaa
     2881 atgaacaaac agaagataac ctaaaaacgc tacttgataa tatgcctgtt ggagtagttc
     2941 aatttgatca agagaccaac gctgtagaat ggtataatcc ttatgcagaa ttaattttta
     3001 caactgaaga gggttttata caaaacagtt tgattcagca aattattacg gaaaaacgtc
     3061 gtgaagatat ttctcaaaca tttgaagttt ctggtaataa atacacttct tatattgatg
     3121 tatcatcagg aattttttat ttttttgata gctttgtggg aaacaggcaa ttagctgatg
     3181 ctagtatgtt aagaccggtt gttggcatta tttcggttga taattatgat gatatcactg
     3241 atgatttatc agatgcagat acctcaaaaa ttaattcatt tgtagctaac ttcattgatg
     3301 agtttatgga gtcaaaacgg attttttacc gtcgtgttaa tatggatcgc tactatttct
     3361 ttacagactt taaaacatta aatgatttga tggataataa attttctgtc ttagaagagt
     3421 ttcgtaaaga agctcaagat gctcaacgcc ctttaacact cagcatagga atttcttttg
     3481 gtgaagaaaa tcatagccag attggacagg tagctcttga gaatcttaat attgcgcttg
     3541 ttcgtggtgg tgatcagatt gtcattcgtg aaaatgcgga tcatacgaat ccaatttatt
     3601 tcgggggagg gtctgtttct acagttaaac gttcaagaac acgtacccgt gctatgatga
     3661 cagctatttc agatcgaatc aagatggtgg acaatgtttt tattgttgga cataggaaac
     3721 ttgatatgga tgcactaggt tcagctgttg gtatgcaatt tttcgcaggt aacattattg
     3781 aaaatagttt tgccgtttac aatccagatg agatgagtcc agatattgaa agggctattg
     3841 agcgattgca ggctgacgga aagactcgtc taatcagtgt ttctcaagca atgggtttag
     3901 tgactccaag atcactttta gtaatggttg accattctaa gatatcttta acactttcta
     3961 aagaatttta tgaacagttt caaaatgtta ttgtagttga tcatcacaga agagatgatg
     4021 attttcctga taatgctatc ttaactttta ttgaaagtgg agcaagtagt gctgcggagc
     4081 tcgtcactga gttaattcaa tttcaaaatg ctaaaaaacg tttaaataag atccaagcca
     4141 gtgttttaat ggcaggtatt atgcttgata ctaaaaattt ttctacgcga gtgacaagtc
     4201 gtacctttga tgtcgctagt tacctaagaa gcaaaggaag tgatagtgtt gaaattcaaa
     4261 atatatccgc aacagatttt gaagaatata agcaaataaa tgaaattatt ttacaaggag
     4321 aacgtcttgg tgacagtatc attgtagctg caggagaaaa aaatcatctt tatagcaatg
     4381 ttattgctag taaagctgca gatacgatac tgtcaatggc tcacgttgag gctagctttg
     4441 tattagttga aacagcttct cataagattg ctatttcagc tagaagtcgt agtaaaatca
     4501 atgttcaacg tgtgatggaa aaattaggtg gaggaggcca ctttaatctt gctgcctgtc
     4561 agttaacgga tatcagtctt ccccaagcga agcacttatt attgaaaact attaatatga
     4621 caatgaaaga aacaggagaa gtagaatcat gaaagttatt tttttagcag atgtaaaagg
     4681 aaaaggtaaa aaaggagaaa tcaaagaggt cccaacaggc tatgctcaaa atttccttat
     4741 taagaaaaat ttagctaaag aagcaactag tcaaagcatc ggtgaattaa aaggaaaaca
     4801 aaaagctgaa gaaaaagctc aagctgaaat attagcagaa gctcaagctg tcaaggctgt
     4861 tttggatgag gacaaaacac gtgttcaatt tcaagaaaaa gttggtccag atggacgtac
     4921 atttggttcc atcacagcta aaaagatttc agaagaattg caaaagcaat ttggtgtaaa
     4981 agttgataaa cgtcatattg ttttagatca tcctatccgc gccattggtc taattgaagt
     5041 tcctgtgaaa ttacataagg aagtaacagc tgagattaaa ttgactatca cagaagcata
     5101 atataaaaaa catcacaaga tttggaggtt atgaggttgc ctgaagtagc tgaattacga
     5161 gttcaacccc aagatttact agcagagcaa tctgttcttg ggtcaatctt tatctcacct
     5221 gataagctga ttgcagtgag agaatttatc agtccagacg atttttataa gtacgctcat
     5281 aaaattatct ttcgggcaat gattaccctc agcgatcgta atgatgccat tgatgcaacc
     5341 actataagaa caatcctaga tgatcaagat gatctgcaaa gtattggtgg cttatcctat
     5401 attgttgaac tagttaatag tgtcccaact agtgctaatg cagaatatta tgctaaaatt
     5461 gtagctgaga aagctatgtt gcgtgatatt attgctaggt tgacagaatc tgtcaaccta
     5521 gcttatgatg aaattttaaa accagaagag gttatcgctg gagttgagag agctttaatt
     5581 gaactcaatg aacatagtaa tcgtagtggg tttcgcaaaa tttcagatgt gctaaaagtt
     5641 aattacgagg ctttagaagc acgttctaag cagacttcaa atgttacagg tttaccaact
     5701 ggttttagag accttgacaa gattacaaca ggtttacacc cagatcaatt agttatttta
     5761 gctgctcggc cagcagtggg gaagactgcc tttgttctta atattgcgca aaatgtgggg
     5821 actaagcaaa aaaagactgt tgctattttt tctttggaaa tgggtgctga aagtttagta
     5881 gatcgtatgc ttgcagcaga aggaatggtt gattcgcaca gtttaagaac agggcaactc
     5941 acagatcagg attggaataa tgtaacaatt gctcagggag ctttggcaga agcaccgatt
     6001 tatattgacg atacgcccgg gattaaaatt actgaaatcc gcgcaagatc acggaaattg
     6061 tctcaagaag tggatggtgg tttaggtctt atcgtaattg actacttaca gttgattaca
     6121 ggaactaaac ccgaaaatcg tcagcaagag gtttcagata tttcaagaca gcttaaaatc
     6181 ctagctaaag aattgaaagt accagttatt gccctaagtc agctttctcg tggcgttgag
     6241 caaaggcaag ataaacgacc agttttatca gatattcgtg aatcaggatc tattgagcag
     6301 gatgccgata ttgtagcctt cttataccgg gacgattatt accgtaaaga aggtgatgat
     6361 gctgaagaag ctgttgaaga taacacaatt gaagttatcc tcgagaaaaa tagagctggg
     6421 gcgcgtggaa cagtcaaact gatgtttcaa aaagaataca acaaattctc aagtatagcc
     6481 cagtttgaag aaagataaga aagagagaca aaaaatgagc gatgcattta cagatgttgc
     6541 aaaaatgaaa aaaattaaag aggacataag agcccatgaa ggacagttag tagaattaac
     6601 ccttgagaat ggacggaaac gggaaaaaaa taaaattggt aggcttattg aggtttacag
     6661 ttcgctcttt atcattgagt atagtgatag ctctgatact ccaggagcaa ttgataattc
     6721 ttatgtcgaa tcatacacct attcagatat tttgacagaa aaaacattaa ttcgttattt
     6781 agattgattt ttgcctcata gaggcttttt ctttacaata aattgatata attattagcg
     6841 atagaagatt attctgcctg tattcttttt attttgaaaa aattcctata aaagataata
     6901 tttttaggtt agattgaagg aaaaaagagg tcttcaccat ggttaagcaa atgatcttag
     6961 tactaaggtt aaaagattat cgtgaaataa agttttgaca caagtgtaat taatagagga
     7021 acttgaagta tccggtaaaa ctatcttgaa gtgaaaaaac gagtagacct cccctgataa
     7081 atacaatata ctagccattt cagagcaatt aagagtttcc agtgaagtgc ttttgataga
     7141 gagctaagaa aaaaacagtc aaggtttaag aactgcaaat tagcatttaa attatttttt
     7201 gttaatgaca gttgtcttta ttaccataac tataacttct tataatatag ccttcttaat
     7261 tcatcagtgg tggttcgtcc catatctgac cttaaaatat taaactgatc atcagatgat
     7321 agatagactg actttacaaa acaactttct tatggtaaac tgttctttgt gcgaaataac
     7381 agcagaaaaa tatgaagctc gtcaacaggt ggttatctat tcaagtaacc tttgctgttt
     7441 aggcgaaaac cccctgcacg aatcaggatt ttttaaaccg ttatttcctc aaaaaattta
     7501 gaggaggaca tgtaatatgt cacgttatac tggtccatca tggaaacaat cacgtcgcct
     7561 tggtttatca cttacaggca caggtaaaga attggcacgt cgtaactacg ttcctggtca
     7621 acacggtcca aacaaccgta gcaaactctc agaatatggt ttgcagttag ctgagaaaca
     7681 aaaacttcgt ttctcatacg gtttaggtga aaaacaattc cgtaacttgt tcgttcaagc
     7741 tacaaaaatc aaagaaggaa ctcttggttt caactttatg gttcttttag aacgtcgtct
     7801 tgacaacgtt gtttaccgtc taggtttagc aactactcgt cgtcaagcac gtcaattcgt
     7861 taaccacggt cacatccttg ttgacggtaa acgcgttgat atcccttcat accgtgttga
     7921 tccaggtcaa gtgatttcag ttcgcgaaaa atcaatgaaa gtacctgcaa tccttgaagc
     7981 tgttgaagct actcttggac gtccagcttt cgtatcattt gatgctgaaa aacttgaagg
     8041 ttcattgact cgccttccag aacgcgatga aattaaccca gaaatcaatg aagcacttgt
     8101 cgttgaattc tataacaaaa tgttgtaata ttttattgaa taggataggc ttaaaagcct
     8161 tgatactaag cactttgggg cattattcca aggtgctttt tttgatttta ctaccctttt
     8221 agttctcttt agtcggattt tagtatagta agagggtagc cccaaagtgg ggcacccttg
     8281 ttaattataa attgctaata gctgtctcaa aaatcgagac ggctttttta gcgccttctt
     8341 ttgttgcatg gacataagta ttcaatgtca ttgagatatt agagtgtcct agtctatatt
     8401 gcaagtcttt agcctctatc ccagcgtaaa gcataattgt agcgtgggtg tggcggaagc
     8461 cgtggaaact aatatcaggc acgccagcag ctttaaaatg cttttgaagc ctttttctaa
     8521 gcaaacaagc gtaggcgtat tttgtggtaa acggagtaaa aacaatcgtc tcagaccgtc
     8581 ctagatccca agataaaact tgctgacggt ttttatattg cttaagaagt aagacggtag
     8641 ctctatcaat ctcaatgtct cttaatcctg cttttgattt aggagtgttt gtctcctgat
     8701 atctattcaa agttttggaa accttgatag ttcctttttt tagatcaata tcagaccatt
     8761 caagagctag aacttctcca atccgacaac cagtagcaag caaggtctta taaagaacgt
     8821 aatcaaagaa gttttcatag ctagataaat ctaagtcatc taaatagttc aagaattgct
     8881 ttagttcttg attgctgaag aactttacct tatgttcttt gttattttgt tttcgaggaa
     8941 tgatgacgtc acgagctgga ttatgttcaa taacttgcat agtaacgcca tattgtagaa
     9001 tacggcgatt gatattattt agaaaactgt agttagcata tgcgccttta atgcccttat
     9061 tagcgttgtt agcccacttg ttaacttgtt gctgaataac aggggtagtt agcttagata
     9121 gcttgcaatc cccaaatgcg ggcaaaatat gaagtcttac aatcccttcc atagattgcc
     9181 gagagtttgg tttgatcgta ttcttgtaac tctcccacca aagggctgtt agctctttat
     9241 aagtggttac tgtcggcttc tctttgacgg tatagccatt catagcaaag ttatttagag
     9301 catcacgagc ttttatttta accccttttt tagttgaagc tgtaatagtt gtgcgagcct
     9361 tcttacctgt tacctggtcg acaccaagat agacactagt gcggtaaatg attgtaccgt
     9421 tcttcttttt gtattctttt atgttcatga taataccttt ctaacatcag taagcaagta
     9481 tgggatttag ttaggtattt aggttttatc atgggtgata gttggttatc tggtagggtg
     9541 gcgataaggt agagggtgtg ttaatgctat ggttgtcaat ataagtgttg attattgttt
     9601 ggtgggatag ttgcgagctg gtcggtatca gggagatttt ggagagaagt ttttgctttc
     9661 tttttgcaac tcccacatgt ctctagtaaa ttttctaact tgatttttat agggagtttc
     9721 aagctgtctg taattatata ataggtcgtg ttcatcagat tcggttgcgg tcatgtcgaa
     9781 ggaaaatgaa tcaccttcag agttaattcg cttgttcatt tctgctaaaa aattcgcttg
     9841 ctcttctgtt cggatattgc tatttcctag aagataaggg agccatactc caaaatagtc
     9901 cgccagtatt tgagctttat caggcttgat tgtattcttt gggttttccc aattttgata
     9961 tgttctaaat gaaatgtcta gatttaattt cttatttacg acttgatcaa aaaaatcttt
    10021 ttgattgaga tttgtttgtt cttttcttat tttttgtagc ctattttc
//
LOCUS       DATUYR010000050         9962 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.050, whole genome shotgun sequence.
ACCESSION   DATUYR010000050 DATUYR010000000
VERSION     DATUYR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.050"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            15..752
                     /locus_tag="VCI33_001315"
     CDS             15..752
                     /locus_tag="VCI33_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP2832236.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALVDQA
                     DALQSEEAAVVQSDTAASDALEALADQTDALQSEEAAVVKADTAASDALEALADQTDA
                     VQSEEASVVKADTAASDAWEKAATPTALDVKKTKDTTPVVKKEERQNVNTLPTTGEES
                     NPFFTAAALAITVSTGALVVSSKCKEN"
     gene            990..1532
                     /locus_tag="VCI33_001316"
     CDS             990..1532
                     /locus_tag="VCI33_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP2832237.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1541..2824
                     /locus_tag="VCI33_001317"
     CDS             1541..2824
                     /locus_tag="VCI33_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP2832238.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCENSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2840..3700
                     /locus_tag="VCI33_001318"
     CDS             2840..3700
                     /locus_tag="VCI33_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEP2832239.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTRSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3702..4667
                     /locus_tag="VCI33_001319"
     CDS             3702..4667
                     /locus_tag="VCI33_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEP2832240.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4778..6061
                     /gene="aroA"
                     /locus_tag="VCI33_001320"
     CDS             4778..6061
                     /gene="aroA"
                     /locus_tag="VCI33_001320"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEP2832241.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            6054..6545
                     /locus_tag="VCI33_001321"
     CDS             6054..6545
                     /locus_tag="VCI33_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP2832242.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSIIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6753..8204
                     /locus_tag="VCI33_001322"
     CDS             6753..8204
                     /locus_tag="VCI33_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP2832243.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDTPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8286..9920
                     /gene="rlmD"
                     /locus_tag="VCI33_001323"
     CDS             8286..9920
                     /gene="rlmD"
                     /locus_tag="VCI33_001323"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP2832244.1"
                     /translation="MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMR
                     VLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDS
                     LYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYY
                     IQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPK
                     VFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAI
                     SAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEV
                     IEAAVRDAQHNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFI
                     QASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVETVALLSK
                     LGVDKHINIEIEMDELDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKRKCGIEL
                     REHYNKSKKEKQIIPQCTPEKEEAIMDALRHFKMIE"
BASE COUNT         3286 a         1679 c         2058 g         2939 t
ORIGIN      
        1 aaaaaggaga aacaatggga aaagaaataa aagtgaaata ctttttgcgt agatcagctt
       61 ttggattagt tgcggtgtca gcatcagtat tagtcggttc aacagtatct gctgttgact
      121 cacctatcga acagcctcga attattccaa atggcggaac cttaactaat cttcttggca
      181 atgctccaga aaaactggca ttacgtaatg aagaaagagc cattgatgaa ttaaaaaaac
      241 aagctattga ggataaagaa gctacgacag ctatagaagc agcaagttca gatgccttag
      301 aagcattagt ggatcaagca gacgctttac aatcagaaga agctgcagta gttcaatcag
      361 ataccgctgc tagtgatgcc ttagaagcat tggcggatca aacagacgct ttacaatcag
      421 aagaagctgc ggttgttaaa gcggataccg ctgctagtga cgccttagaa gcattggcgg
      481 atcaaacaga cgctgtacaa tcagaagaag cttcagttgt taaagcagat accgctgcta
      541 gtgacgcctg ggaaaaagca gcaactccaa ccgctttaga tgttaagaaa actaaagata
      601 caacacctgt agttaaaaaa gaagaaagac aaaacgttaa tacccttcct acaactggtg
      661 aagagtctaa cccattcttt acagctgctg cgcttgcaat aacggtaagt acaggtgcgt
      721 tagttgtaag ttcaaagtgc aaagaaaatt agttgctatt tgttctagca aatgaaacaa
      781 gagaatcgaa agattctctt tttctgatat ttaaaaatgc tacagtttat aatagcttga
      841 aagactaaat gttctcaaac tcattagaac aagctttaat aaaactaacc cagttgtatc
      901 aaaggataga taaagactaa gactgttata gaatatttgc aaaagatttg ttataatgga
      961 cccaatacag gtattgtgaa aggtataata tggatatttg gacaaagctt gcagtgtttg
     1021 ctttttttga gactccgaaa gtaatattac ggccttttcg ctatgaagat cattgggatt
     1081 tttacagcat ggttaacgac actaaaaacc tttattatgt ttttccagaa caaaaaacta
     1141 aggcagcaag tgactatctt ttagtacata gttttataaa gtttccttta ggtcagtggg
     1201 caatagaaga taaagcaacc caccaagtaa taggttctat tagaattgag cattatgatg
     1261 ctaaaacgcg ttgtgctgat attggctatt ttttaaacta tgccttttgg ggacaaggaa
     1321 ttatgacaga agtcgtaata aaacttgttt atttatcttt tcacgaattt ggtctcaaaa
     1381 cgttgcgtat tataactcat ttagaaaata aggccagcca gaaagtagct aaaaaagcag
     1441 gttttcaact aaaaacctgt tttaaaggaa gtgatcgtaa tactcataaa atctgcattt
     1501 ataaaatgta ccaactaact aatgataggt gataatcatt atgagcaaac atcaagatat
     1561 tttagattat ttagaaaaac ttgctattgg taaaaaagtg agtgttagaa gcatttcaaa
     1621 ccacttaaaa gtaagcgatg gaacagccta ccgtgctatt aaagaggcag aaaatcgagg
     1681 tattgtcgaa accaaaccaa gaagtggaac tgttcgtatt gaaaaaaaag gacgggttcg
     1741 tattgaccgt ctgacctatt ccgaaattgc tcggatcagt gattcggaag tgctagcggg
     1801 acatgctggt ttaggacatg aattcagtag gttttcaatt ggtgctatga ctcaacaaaa
     1861 tattcgtcgt tacttggtca aaggagggtt attaatagtt ggggatcgtg aaacgattca
     1921 actcttagct cttgaaaacc ataatgccat tttggtgacg ggcggatttc cagtatccaa
     1981 acgtgttatt gaaatggcaa ataaccaacg aatcccagtt atggtaactc attatgatac
     2041 ctttactgtg gcaacaatga ttaatcacgc gctatctaat attcgtatta aaacagatct
     2101 caaaacagtt gaacaggtaa tgatacctat tacagattat ggttaccttt gcgaaaacag
     2161 ctctgttgaa gaattcaata cattaataaa aaaaacacga caagtcaggt ttcctgtatt
     2221 agattataaa agaaaagtga ttggtgtagt tagtatgcgt gatgtggtgg atcagttgcc
     2281 aacaactaag cttaccaagg tcatgtctaa aaatccaata acggctagac caaatactag
     2341 tcttgctaat attagccaga aaatgatctt tgaagattta aacatgttac ctgtcactga
     2401 cgaagaaaac aatttacttg gtatgattac tcgtagacaa gcaatggaaa atttgccaaa
     2461 tcaccaaccc aataacccct atacttatag tgagcaaata ctttctaacc ttgaagaaac
     2521 ggttgattat taccaagtag tagttgagcc aacaatgatt gatagtgctg gtaacatgtc
     2581 aaacggtgtt atttcagagt tcttaaagga aattagtatc agagccctca cgaaaaagca
     2641 ccaaaaaaat atcattatcg aacaaatgat ggtatacttt ttacatgcta ttcaaattga
     2701 ggatgaatta aaaatatatc ctaaaatcat tactgaaaat agacgaagca gcaccattga
     2761 tattgaaatt tttgtggatg atcaagtgat tgctaaagct attattacaa cgaaaataaa
     2821 ttagaggagt aacaaaacaa tgataacatt aaaatcagcc cgtgaaatag aagcgatgga
     2881 tagagcagga gacttcttag caggtatcca tattggctta agggatatta ttaagcctgg
     2941 tgtggatatg tgggaagtgg aagcatatgt tcgtcgccgt tgtaaagaag acaacgttct
     3001 tcctttgcaa ataggggttg atggtcacat gatggactat ccttatgcga cctgttgtgg
     3061 tctaaatgac gaagtagcac atgcctttcc ccgtcattac attctgaaag aaggggattt
     3121 gcttaaagtt gacatggtac tcagtgagcc tttggataaa tcaattgttg atgtagcagc
     3181 tttagatttt gataatgtgc ctgagatgaa gaaatggaca agatcttata ctggtggttt
     3241 agctgactct tgttgggctt atgctgttgg aacaccgtct gacgaaataa agcaattaat
     3301 ggatgtcacc aaagaagcca tgtaccgtgg tattgaaaaa gcagtaattg gcaatcgtat
     3361 tggtgatatt ggcgcagctg ttcaagaata cgctgaaagc ttcggttatg gtgtcgtgcg
     3421 tgatcttgtt ggtcacggtg ttggtcctac aatgcatgaa gagccaatgg taccaaacta
     3481 tggaacagca ggtcgtggac ttcgtctaaa agaaggcatg gtcttgactg ttgagccaat
     3541 gattaataca ggaacttggg aaattgatac cgacatcaag acaggttggg ctcataaaac
     3601 cctagatggt ggtttatctt gccaatacga acaccaattt gtgattacta aagatggtcc
     3661 cgttatttta acaagtcaag gagaagaaag aacttactaa aatggcagaa aaaaaatggt
     3721 ttgataaggt actgtccaag tggcagtatg aacccattca agtctttatg aggcatttac
     3781 aaagtgcaga aatggattta tctgccattg cggttgcgta ttaccttatc ttaacggcct
     3841 ttccattaat tgttattgca gccaatattt ttccttacct caatattgat attgctgatt
     3901 tgttacgttt aatgaagcaa aatttgccta aagatatttt tagaccggct tctgcaattg
     3961 tagaaaatat tttctctaaa ccgtctggaa gtgttttggg agtagcgact ttgacaggat
     4021 tatggacaat gtctcgaagt ttgacatcgc tgcaaaaagc tattaataaa gcctatggcg
     4081 cttctcagca tcgagatttc tttatcggac acttggttgg tttgttgacc agtttaatta
     4141 ttttattttt attagccttt gccttaatct tttcaacatt ttctaaagct gctattcaag
     4201 tgctggataa gcattaccat ttgagtgata atatcacaac gattttctta ctcctcattc
     4261 aacctattac agttttgatt atttttgttg gcttgatgct actatatttt ttattaccca
     4321 atgttaagat taaaaaaatt cgctacattt taccaggaac cctatttaca tcttttgtga
     4381 tgacattttt gagtaatctg gttggcaatt acgttgtgta taatgttgag cggatggtag
     4441 atattaaaat gtttggttca gttatgattt ttatcatcat gctatggttt attttcttag
     4501 caagaatcct gattttagga gctatattta atgcgaccta tcaagaaatg tccttgggga
     4561 aattagaagg tcgtagtggt gatatgatcg ctatccttaa aaaaacactt gggaatgatt
     4621 cagatctatc tccttcacag tctatcgaag atagtcacac tgattgatac aaaaaagata
     4681 ataagtcgtg gagtgagaaa ctattttctt gcttcatttt ttatgaggat aaatagaaaa
     4741 tcattgatct tagcaaatag aaaggcagat aaaacggatg aaattgcgaa caaatgcagg
     4801 accattacag ggaacgattc aagttccagg agacaagtcc attagtcacc gggcagtcat
     4861 attaggagct gttgctaaag gggaaacacg agtaaaagga ctcttaaaag gagaagacgt
     4921 gctttccacg attcaagcct ttaggaattt gggggtccgt atcgaagaaa aagatgacca
     4981 gcttgtgata gaaggtcaag gttttcaagg attgaccgct ccttgtcaga cacttaatat
     5041 gggaaattct ggaacctcta tgcgcctcat tgctggctta ttggcaggtc aaccttttag
     5101 tgtcaaaatg attggcgatg aaagcttatc caaacgtcct atggatcgga ttgtttatcc
     5161 gttaaagcaa atgggggttg agatatctgg agaaacagat aggcaatttc ctccactaca
     5221 attgcaaggt aatcgtaatt tacaaccaat cacatatact ttgcctatct cttctgctca
     5281 ggttaagtca gctattttgt tagcagcttt gcaagcaaaa ggaacaacac aggttgttga
     5341 aaaagagatt actcgaaacc atacagaaga aatgattcaa caatttggtg gacgattgat
     5401 agttgatggt aaacgcatta ctttggtggg accccagcag ctcactgctc aagagataac
     5461 tgtccccgga gatatttcta gtgcagcttt ttggcttgtc gccggcttaa ttattcctgg
     5521 ttcggagctc cttttaaaga atgttggagt caatccgaca cgaacaggga ttcttgaagt
     5581 ggttgaaaaa atgggagcac agattgtcta tgaagatatg aataagaaag agcaagtaac
     5641 tagtattcga gtggtatatt ctcacttgaa aggaactatc atatcaggag gtcttatccc
     5701 tcgattgatt gatgaacttc ccatcattgc acttcttgca acccaagcac aaggaacaac
     5761 ctgtattaaa gatgctcaag aattacgagt caaagaaaca gatcgtattc aagtagtaac
     5821 tgatattctt aacagtatgg gagctaatat caaagcgaca gcagatggca tgattatcaa
     5881 aggccctacg gtactatatg gggctaacac aagtacctat ggagatcatc gtattggtat
     5941 gatgacagca attgcagcct tgcttgttaa acaaggacag gtccatttgg ataaagaaga
     6001 agccatcatg acgtcttatc caacgttttt caaagattta gaaaggctgt gtcatgacta
     6061 aagtgttatt gggatttatg ggagtgggga aaacaacggt ttcgaaacac ctaagtatgc
     6121 actgtaaaga tatggatgct attattgaag ctaaaatcgg tatgtctatt gcagcttttt
     6181 ttgaacagca tggagaaata gcatttcgaa caattgaaag tcaagtgtta aaagatctat
     6241 tgtttgcaaa tgataatagt atcatcgtaa caggtggcgg tgtagtggtt ttacaagaaa
     6301 atcgacaatt actgagaaaa aatcaccagc ataatatttt attggtagct tcttttgaaa
     6361 ctttgtacca gcgtttaaaa catgataaaa agtctcaaag acctctattt cttaagtact
     6421 ctaaggaagc attttatgaa ttttatcaac aacgtatggt attttatgag ggactttcag
     6481 atttagtaat cagagttgac catcgtacgc ctgaagaagt tgccaacatc attgagggtt
     6541 attagtaggt cattgtcgta atctgatatt tatggtttta aacaggtatc tgtctaaagc
     6601 acttcttatg gcaagttggc tttattaaat attaggaaat aatcttgatt attctataat
     6661 gattaaaatt ttgagaggac aatctggcta atataggtta taatggtagt attcgttaaa
     6721 agtgcaattg taatactaag agatgataag atatgacaaa ataccccatg ggtggcctta
     6781 gtcaccatga agaattacgt tatttttatc tcctaaggaa tttgagttac cttagtgaga
     6841 atgaaaaaaa agagtttgct tttttaaaat ctaaattgga aattggtcga gcttatgctc
     6901 cttctaagca gcattatagg aagtcaaaac ggcaagaacc ttactttgaa gatgactact
     6961 ataacgacta ctcaccaaat gatttactag aagatgatga cgtcaatcat gactcttctt
     7021 ttgttccata tggtttaccg atatatccta aagaagacag atatttaaac aaaaaaacaa
     7081 aacttacagc tcgtagacct atagatactc cgcagcctat tgatgaagac gatgcttttt
     7141 taacagagtc tgttgctcgt tgtgctcttc ctagatctca aaaaagaaag cataagaaaa
     7201 aaggatgtat gaaatggttt tttaacattc ttggcttatt gctaatgact gtgcttatgg
     7261 ggttagggtt gatgtttgca aaaggggttt ttgatatttc tactaataaa gctaattaca
     7321 aaccagctgt ttcccaagct tttgatggtc aagaaactca agatggtacg aacattctgg
     7381 ttttgggttc tgaccaacga gttactcaag gttctacaga tgctcgcaca gataccatta
     7441 tggttgtcaa tgtcggcaac catgctaaaa aaataaaaat ggtctctttt atgcgtgaca
     7501 ctttgattaa cattccagga tacagttata atgataattc gtatgatctt aaattaaata
     7561 gtgcatttaa tcttggagag caagaagatc accacggagc agagtacgtt cgacgggcct
     7621 taaaacataa ttttgatatc gacattaagt attatgtgat ggtagatttt gaaacatttg
     7681 cagaagctat tgacaccttg tttccaaatg gtgttaagat tgatgccaaa tttgcgacag
     7741 taggtggtgt agctgttgac tccgtagaag ttccagatga cctgcgaatg aaaaatgggg
     7801 ttgttcccaa ccaaaccatc gaagttggag aacaacgcat ggatggtaga accttgctca
     7861 actatgctcg tttccgtaaa gatgacgaag gagatttcgg acgaaccgtc cgtcagcaac
     7921 aggttatgtc agctgttatg tcccaaatca aagacccaac taaactcttt acagggtctg
     7981 cggctattgg taaaatctat gcgctgactt cgaccaatgt ttcttttcca tttgttgtta
     8041 aaaatggtgt ttctgtttta ggaagtggga aaaatggagt tgagcacgtg accatcccag
     8101 aaaacgggga ttgggttgac gaatacgata tgtatggagg acaagccctt tacatagatt
     8161 ttgataagta tcagaaaacg ttagccaaat taggtctgcg ctaagatatg gtataatgga
     8221 gagtaactaa agttgctctt cttttttgag caatggttag ccctagaaaa ggaaaaagaa
     8281 taagaatgtt gaaaaaaaat gatattatcc aagttgctat ttcagattta agccatgagg
     8341 gagcaggtgt cgctaaacac gatggttttg ttttctttgt tgataatgcc ttgccagaag
     8401 aagtgattga catgcgtgtc ttaaaggtca ataaaaacag cggttttggc aaagtagaag
     8461 cctatcatta cctgtcgcca gctagaaatg cagatgtcaa tttaacttat ttgcgaacag
     8521 gcattgcaga ccttggacat ctaacttatg aagaccaact gactttcaaa aagaaacaag
     8581 ttcaggacag cctctataaa attgctggaa tcagtgacgt gacagtcgaa agcacgattg
     8641 ggatgacaga acctttagct taccgaaata aggctcaggt tcctgttaga cgtgttaatg
     8701 gtcagctaga aacaggcttt ttccgcaagc attctcatga cttgattcct atttcggatt
     8761 attatattca ggacaaagag attgatcgtc tcattaattt tacccgtgat ttattacgcc
     8821 gctttgatat taaaccgtat gacgaaacag aacaaacagg tctcttgaga aatattgttg
     8881 ttcgccgagg ccattactct ggagaaatga tgctggtctt agtgactaca cgcccaaaag
     8941 ttttccgtgt tgaccaagtc attgaaaaaa tcgttgaagc ttttccagca gtggtgtcta
     9001 ttattcaaaa tattaatgat aagaacacca atgcaatctt tggtaaagat ttcaaaacgc
     9061 tttacggcaa ggataccatt actgatagca tgcttggaaa caactatgcc atttctgccc
     9121 agtctttcta ccaagtgaat acggttatgg cagaaaaact ctatcaaaca gctatagcct
     9181 tttcagacct ttctaaagat gacattgtga ttgacgccta ctcaggtatc ggaaccattg
     9241 gcctatcatt tgctaaaaca gttaaagctg tttatggtgt tgaagtgatt gaagcagctg
     9301 ttagagatgc tcaacacaat gccgctctta atggcatcac caatgcctac tttgtcgcag
     9361 ataccgcaga acatgccatg gcaacatggg cgaaagatgg cattaaacca agtgttattc
     9421 tggtagaccc gccacgaaaa ggcttaacag aaagtttcat ccaagccagc gtggcaatgg
     9481 gaccacaaaa aattacctac gtttcctgca acccagccac catggcgcgt gacatcaagc
     9541 gttatcaaga gttgggttac aaactggcca aagtacagcc cgtagatttg tttccccaga
     9601 cgcatcacgt ggagacggta gcacttttgt ccaaacttgg tgtcgataag catataaata
     9661 ttgaaattga gatggatgag cttgatttga caagtgctga aagtaaggca acatatgctc
     9721 aaatcaaaga atatgtttgg aataaatttg aattaaaagt ttcgacatta tatattgcac
     9781 agataaaaag gaaatgtgga atagaattac gagaacatta caacaagtct aaaaaggaga
     9841 aacaaattat tccacagtgt acacctgaaa aagaagaagc catcatggat gctttgagac
     9901 atttcaaaat gattgaatag aaaagaatga cagcatatca ctttctgcat ttattacatt
     9961 cc
//
LOCUS       DATUYR010000051         9241 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.051, whole genome shotgun sequence.
ACCESSION   DATUYR010000051 DATUYR010000000
VERSION     DATUYR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.051"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..897
                     /locus_tag="VCI33_001324"
     CDS             <1..897
                     /locus_tag="VCI33_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP2832245.1"
                     /translation="KAERIPLEGRSKAQRATEIIQALRNQFPLEMLLEILDLSRSTYY
                     YQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVM
                     SLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELALPEGKLYLLP
                     VLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSYSGTILHSDQGWQYQHQSYHDFL
                     ESKGILPSTSRKGNSPDNGMMDSFFGILKSEMFYGLETTYQSLDKLEEAITDYIFCYN
                     NKRIKAKLKGFSPVQYRTKSFQ"
     gene            complement(946..1677)
                     /locus_tag="VCI33_001325"
     CDS             complement(946..1677)
                     /locus_tag="VCI33_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP2832246.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVVGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQRTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
     gene            1792..2157
                     /locus_tag="VCI33_001326"
     CDS             1792..2157
                     /locus_tag="VCI33_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEP2832247.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            complement(2277..3497)
                     /locus_tag="VCI33_001327"
     CDS             complement(2277..3497)
                     /locus_tag="VCI33_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2832248.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLSL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            3871..5355
                     /locus_tag="VCI33_001328"
     CDS             3871..5355
                     /locus_tag="VCI33_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEP2832249.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLKKNGIASYLHYPIKSYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCISQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            complement(5460..5861)
                     /locus_tag="VCI33_001329"
     CDS             complement(5460..5861)
                     /locus_tag="VCI33_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEP2832250.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(5946..6727)
                     /locus_tag="VCI33_001330"
                     /pseudo
     CDS             complement(5946..6727)
                     /locus_tag="VCI33_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            complement(6879..8558)
                     /locus_tag="VCI33_001331"
     CDS             complement(6879..8558)
                     /locus_tag="VCI33_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP2832251.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRKQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGAGMFGNKCTKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFRKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKIIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLITAL
                     ISEIQVYEEKQPNGQWLKSITFKLPIIDDDLNISLDNNEQVECVSLLEKRS"
     gene            complement(8655..8816)
                     /locus_tag="VCI33_001332"
     CDS             complement(8655..8816)
                     /locus_tag="VCI33_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832252.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     KAKTQIETD"
BASE COUNT         2800 a         1995 c         1587 g         2859 t
ORIGIN      
        1 aaagctgaga gaataccgct tgagggacga agtaaagctc aaagagcaac agaaatcatt
       61 caagcattaa ggaatcaatt tcccttagag atgctacttg agatccttga tttatcacgc
      121 tccacctatt actatcaagt gaagcgacta gctcaaggag ataaggatat agaactaaag
      181 catgtgattc gagagattta tgatgagcat aaaggtaatt acggttatcg tcgtattcat
      241 atggagttgc gtaaccgagg atttgtggtc aatcataaga aggttcaacg tctcatgaaa
      301 gtcatgagcc tagcagcccg cattcgtcgg aagcgcaagt attcttctta caaaggagag
      361 gttggtaaga aggctgataa tctgattaaa cgtcacttcg aaggctctaa accttacgaa
      421 aaatgttaca ctgatgtgac ggagttggcc ttacctgaag gaaaactcta cttattacct
      481 gttcttgacg gctataacag tgagattatt gatttcactc tgtctcggtc tcctaacttg
      541 aaacaggttc aaaccatgct tgagaagact tttccagcgg attcatacag cggaactatt
      601 cttcatagtg accaggggtg gcaataccaa caccagtcct accatgattt tttagagtct
      661 aaaggtattt tgccatccac gtcccgcaaa gggaatagcc cggataacgg catgatggat
      721 tccttctttg gcattctcaa atctgaaatg ttttatggac tcgagacaac ttatcaatca
      781 cttgataagc ttgaagaagc tattacagat tacatttttt gctacaacaa caaacgaatt
      841 aaagcaaaac taaaaggatt tagtcctgtc caatacagaa ctaaatcctt tcaataatta
      901 tttgtccaac tttttggggt cagtactgac aggggaggtt tctttttata gtgagctctg
      961 acaataaggt gccaggcttt gcaattcctc aggcgtcagg cgcctaaact ccccttctgc
     1021 aaggctaggg tctagtttta atggtcccat gctgagacgc tgcaagtcaa gcactccctt
     1081 tccacaggct gctaccatgc gtttcacctg atggaatttt ccttcttgta tggtaatttt
     1141 aaccaaactt gttcgctgct gtaaatcact agcaagcact tctaaatgag caggtaggca
     1201 ctggtgatcc tttaaggaaa tacctctagc aaaataatct ttatcagctt cagtcatgat
     1261 gcctactact ttagccaagt actctttcgt aacatgtttt ttaggggata aaaggtcatg
     1321 ggctagttgt ccattattgg tcaagagcaa aagtccacgg gtatccttat ctaacctacc
     1381 cacaggaaaa actgcttttt gcctagccgt gtcatctaac aactccataa cagtagcctg
     1441 ctgtctgtcc tgggttgctg acacataccc tgacggcttg tttaacaggt aataaacaaa
     1501 ggattcatag accaagggcg tgccttgata cgtcaccaaa tccttgtatt catcaatgtg
     1561 tgctttggca gaggtttcaa ccttttgatt gacgaaaata gccttctttt ttaagagaag
     1621 tttgacttgg ctcctagttc caacaccagt tgccactaaa aatttatcta agcgcatcat
     1681 aaccctttca aagaaaattc attattaagt ataacataaa aaaacagtag tttcatgact
     1741 gtaagcgctt aatatattat aataggctta tagaaacgag gagattaact tatgactatt
     1801 tattctcaca cttatgaaac ccgtcccaat cattcggcta aagccatggg ctcaggaacg
     1861 ctgaatgtcc tagctactcc tgctcttgtt gcttttatgg aaaatgcttc ttacttattt
     1921 gcccaagaac agctcgacag tgactttaca acggttggca gcgagatagc tatccaacat
     1981 ttggcagctt cagccattgg agatcctgtt acaatcatca ttacagccct taaagaagaa
     2041 ggccgaaaat acgatttccg cttggaagct tttgtgggag aacaactcat tggaaaagcc
     2101 tgtcatacta gagttagggt gaacagtaaa cattttatgg ccaaagtagc ggagtaaaac
     2161 aaaagaaaag aaccaaaaag gtaagcaatc cctaagtgct tacctttttg gttatctcaa
     2221 tcaataaaat agctttgtga gtgaaagtaa ctcaaatact aacacccatc tctttgctaa
     2281 cgcaattgtt ggtagcgttc tgcttttaac tgaccaatag accacatagc tccaaaacta
     2341 atcacataca gagaagctag gaaaatcatg accattgaaa tacctgaggc atctaaaata
     2401 ctaccaatag caacggacga aaatcctcct agagcacggc caacattcaa aataacatta
     2461 ttagccgtgg atcgaatgtg atggggataa agtctggtaa tcatagcccc gtagccagca
     2521 aacatcccat tgacaaaaaa accgacaata gcacctccaa taaccatact cgccatggaa
     2581 ttggcaaatt gaaaaaggta aacacaaata gatgatgcta ataaaaagag ggaataaatc
     2641 agacgtggac caaagcaatc taaaagctga ccaaaacaca gcatccccaa acacatgccg
     2701 acaatagtag ccaccatcca caaggacgaa ctttttactg aaaggtttaa gcttgtttga
     2761 ataatggttg gcaaccaatt catcattcca aaatagcccg caatctgcac agtcgtcatt
     2821 accattaaag caagggtttg agctgttaag gattttgttt tgaatagttc attgatttta
     2881 attggttgac tgcattcttc ctcctcttgc ccataatgat cccaaatttt ttggtcatct
     2941 atggctaaag tcatccaaat caccaagaga atcggaagta atccaaagag aaaaaggcca
     3001 cgccagccta gacttggagc cagccaccct gctaaaagag ccgaactaat ggagccaagc
     3061 tggccagcaa taccatttaa agaagacatc cgacccattt tttctggtgg tacaatccct
     3121 gccattatgg caatggctac accgtactca cctccaactc ctatgccagc aataaatcgc
     3181 atcaggtaca ggtagggcaa agactgggta aaataaatta atcctgttgc tactgaaaac
     3241 agtaaaatcg tccatttaaa gaccttaaac ttgtggtgcc tatcagccag caacccgaat
     3301 aagaggcctc ccaccaacat tcctaaattg gtaatggtcg caatccagcc accctgagtg
     3361 ccactaagac tcaagtctgt cataatggaa gacatggaaa aggcaagaaa catgacattc
     3421 aagtcatccg ttcctgaagc tgcaatagct gccactaaag ctcgcttatt tgtggtgtcc
     3481 aatgataatg ttgacataat tgtctccttc ttaatagtag ctcactgact accttaatta
     3541 attgaatacc ctttgtccaa aggtcaaaaa aagacttgga aacaagtccg caagtccatc
     3601 aaagttcact aaataataag agatgacaaa aaaactctct ttaatgttaa gactttcatg
     3661 cttcacggaa catgtttaaa gtagtattta attgttacta gtatagtaaa gctcatttta
     3721 attgtcaagt tcttttgaac atagcggata catttttctt ctaagaacca agcctaagtt
     3781 tttttaaaaa gtcacaaaat gaggtatccc tgcaaaaaac cttttctttc ttggaatttc
     3841 tttgttataa tgctttacag aggtgaaaaa atgaaaacta ttgcttttga ttctaacaag
     3901 tatttaaact tgcaaagaga tcatatttta gaacgtatca gccaatttga tggcaaactt
     3961 tacatggaat ttggtggtaa aatgttagaa gattaccatg ctgcccgtgt acttcctggc
     4021 tatgaacctg acaataagat taaattactc aaagagctca aagaacaagt cgaaattgta
     4081 attgccatta acgctaataa cattgaacat tctaaagcgc gtggtgattt aggcatttct
     4141 tatgaccaag aagtctttcg cttaattgac aaatttaaca cgttagatat ttacgtaggt
     4201 tctgtcgtta ttacccaata caataaccaa cccgctgcag atgcctttcg caagcaactc
     4261 aaaaaaaatg gcattgcttc ttacctgcat tatcctatca agagctaccc gacagatatt
     4321 aatcatatta tctcatctga ggggatgggg aaaaatgatt acatcaaaac gagtcgtaac
     4381 cttatcgttg ttacagcacc aggccctggg tctggaaaat tagccacttg tatatcccaa
     4441 atgtatcacg accaaatcaa tggggttaag tctggctatg ctaaatttga aaccttccca
     4501 gtttggaatc ttcctcttca tcaccctgtc aatctggctt atgaggcagc cacggcagac
     4561 ctagatgatg tgaatatgat tgaccccttc catttggaaa cttatggcaa aacggccgtt
     4621 aattacaacc gtgatatcga ggtctttcct gtcttaaatc ggaccttcga gcgtattttg
     4681 agcaagtctc cttatgcctc accaaccgat atgggtgtta acatggttgg tttttctatc
     4741 gtcaatgaag aagctgctat cgaagcttct aaacaagaaa tcatccgtcg ttattaccaa
     4801 accctcgtgg attttaaggc agagcgggtc acagaatctg ctgtcaaaaa aattgagctc
     4861 ttgatgaatg atatcggtgt cacccctgac gatcgtcacg ttactgtagc tgctcatcaa
     4921 aaagcagagc aaacaggcca acctgccctt gctctccaac taccaaatgg gcaaattgtg
     4981 acaggtaaaa catctgaact ctttggacca actgcagctg ttatcatcaa tgcgattaaa
     5041 accttggcaa agattgataa aaccactcat ttgattgaac ccgaatacgt gaaacctatt
     5101 caagggctca aagtgaacca cttaggcagt cacaatccgc ggcttcattc caatgaaatt
     5161 ctgattgcgt tagccattac tgctatgact agtgaagaag ctaatctagc catgaaagaa
     5221 ctcggcaatt tgaaaggtag cgaagcccat tctaccgtta tcttaaccga agaagataaa
     5281 aatgtactcc gaaaacttgg ggtaaatatt acttttgacc ctgtttacca acatcacaaa
     5341 ctttaccgta aataaactat aaccacccgt ctaacgggtg atttatggta tctctcaata
     5401 aaggaaaaaa gtcccaattc tcagagagtt gggacttttt tcctttattg attcattaat
     5461 cacccgttat gacgtcggcc agtcacaaac atggtcaagc ttgtttttgt tagaagagct
     5521 cctgtttggt tatgaatcgt cacatcaacc acctgcgtgg ttcgacctcc atgaaccaat
     5581 cgaccttcaa ccatcagctt gtctcccttg tgccctgctt ttaaatagtt agcattggcc
     5641 tgtaaggtga cagattctac gccagtagtt ctagcaacca aaccacctac ttgatcacat
     5701 agagtgaaaa gataaccacc atgagcattc ccatagtaat tcaaagccgt ctcagtcact
     5761 tcagtactta aaataaggtg tcctttttca gcaagttcaa tttggtaatt atcaaaaata
     5821 ctaataacat ttaaggtcat ctcttgtgtt aaatctgaca ttgtttttct cttttctaag
     5881 cttagtctaa aagcaataca agcctcatta tactacaaat aggaacagaa ggcagagctc
     5941 atgtttcaaa aatcagtagc ctttccatcc ttttctagcg tattgaacac ttccaagcat
     6001 aaatgaagcg caaaactaaa ggaatcagcg ccccggttgc gttgatcgta aacctctaca
     6061 tctgctagag tatctgctgt aaataagagt tgtccccaaa ggatgcctct taattgagct
     6121 actgctgcta aggcagaaca tcccatctca acaaccgaac acccttcttc ctggcgataa
     6181 gccacttttt ctttggtttc tcggtaaaaa ccatctgttg accaggtgat gacctcttga
     6241 taagctaacc cctgtgctga aagcgttttc tctatcggtc taagcatctt aggatcaata
     6301 tcaatataac aagatggtgc cacgtaatga taacttgttc cttcatctcg caaagcctta
     6361 actggcacta aaaaacggtt ttctgcgata ggaaccaaaa cgccacaggt tcctgtactg
     6421 atcatttctt taaccccata accaatcagc cagtccatga attgcgccgc aggagctgag
     6481 ccaacgggag cttgagctag gcaaatgtct tggcctttat aaggtatctg ataaacaggg
     6541 taaacctttg tggctgaaat gaattctgct acttttttac cttgatggtc taaagcaaaa
     6601 gcttcaatct cgtcttctaa gaaagcatag acacacttag ctgtagacct tcatgtgtag
     6661 gtataatgac ggctgatgga ttactatcac attctaaaag gggaatctcg tgtttggtaa
     6721 taggcattcg tttctcctta tttttgtcat gtggaggcag ctgccttact ccttgattag
     6781 caacactacg cactcgacgt gatgggtctg cgggagtcaa aaggtccagt ggaccttttg
     6841 agcgtctcac cttgaaataa cagagtgagg cggactcttt aactccgttt ctcaagtaaa
     6901 cttacacact caacttgttc attattatcc aaacttatat tcaagtcatc gtctataatt
     6961 gggagcttga aagtaattga ttttagccat tgtccgttag gttgtttttc ttcataaact
     7021 tgaatttcag aaatcaaagc tgtaattaac tgtctacgct ctacatcatt catgacttta
     7081 tagagtttat caaaatagat cagaacctta tatatgttat ctcctgtgag tttttcagct
     7141 tcaatagtct gtttctttgc tttcgcatca attagtgatg attctaattc atctattttg
     7201 tcatacatac gatatagtct atcatctaaa tcctgtttcc ttctcttata gtgcttatct
     7261 tcaacatcta aattatctat ttcctcaatt atcttaaact ttgtagaatg actcttcctc
     7321 aattcctttt ggtaattatc tatttctttt tctatttcag aggtgtccac cttcatgttg
     7381 attttttctt gcatcataga agcaaatttc ggattactta caatcttgac aatcacctct
     7441 gcaacagcat catctaacaa ttcttctcta atttgcttac ggaatgtaca cttatgacct
     7501 cttatcatct gcctatgttt acaaccatag taataaaaat ctttatactt tgtgccatct
     7561 ttctttttct tggtacactt gtttccaaac attcccgctc cacatatagg gcattttaca
     7621 attccagaaa gcaagtgcgt gcgtgtatct tttcctttat tcacatgctc atatttcttt
     7681 gcttgagatt ttagcttaac ctgagcagct tgccaaactt cgtcggaaac tatagcttca
     7741 tgtatacctt cagatattag atattcatct tgttcaacct gcttatattc atttcttgta
     7801 ccatgaactt tttctaaagt tcttcttcca aatgctattt tcccattata tacaggattc
     7861 tttaatatct ttcttataag tcctgcatca aacaaaggat tctttccatt ctgtcttggg
     7921 atttttctaa ttccatgatt ctctaagtat ttagatagcc cattggctcc tatcgtagta
     7981 tttacatact ggtcgaaaat cgttcttatg gcaactgcct cttcctcatt tataaacagc
     8041 ttgccatctt caagtttata tccatacgga gcaaagccac cattccattt tccttccctt
     8101 gctttttgaa tgcgaccttc cattgtttga atacggatgt tttctctttc tatttcagcc
     8161 acagctgata aaacagaaat cattagtttc ccagcatctt tagatgaatc aatgccatct
     8221 tcaacgcaga taagattaac tccataatcc tgcattatct gaagtgtaga aagaacatca
     8281 gcggcatttc ttgcaaatct tgataactta aacacaagaa caaaagacac tccatctttt
     8341 ccagatttta tatcttccat cattcgattg aactgttttc taccttcaat agacttgcca
     8401 gacttcccgg catcttcata ctctccgaca atttcataat cgttgtaaat agcaaaagct
     8461 ttcattcgtg atttttgtgc ctctaacgaa tacccctcta tctgtattga cgtagatact
     8521 cgtgtataga ggtatacttt tattttttct tttgacatag cattaacctc attataactt
     8581 tctatatcat atataaactt tgatttttga atttaagttt ggactatcat ttcaagtata
     8641 ttataacact tttattagtc cgtctcaatt tgtgtttttg ccttgtcaaa actatttttc
     8701 atctcttgat tttttgctgg cgttggatcg ggcagattat ctaaatctaa agcaccagca
     8761 tattttgcaa tcagatttgc tattaaatca gccaatccat tccagtcatt gtccaatata
     8821 tacctcctct ctataaagtt ttatatctaa taattatttg tttaattaag ttttacgaca
     8881 ttgacaagtg ctttggatta gcaacatagg aatctcacct ccgcctctat tccggatgag
     8941 ccggcttcaa ccttagaagt atcattatcc tcattttctt catagcggat agggtatccc
     9001 tccctatatt caaactctta cttatcgctc tctttcttct tccttttttt gcttagcagt
     9061 acttgttttg ccgaattatt cagcagaaag atcttgacgg ataggttatt acgctccaaa
     9121 aataattaac gtcttgtctt ggtctattca attgttaagg ttcaaaattt atcgagagtt
     9181 attaatcttt ttaaaatttg accatcagag aaaatttatc ttgatgtaac aaaattctat
     9241 a
//
LOCUS       DATUYR010000052         9125 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.052, whole genome shotgun sequence.
ACCESSION   DATUYR010000052 DATUYR010000000
VERSION     DATUYR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.052"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(363..1559)
                     /locus_tag="VCI33_001333"
     CDS             complement(363..1559)
                     /locus_tag="VCI33_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2832253.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMVIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVLS"
     gene            1725..2384
                     /locus_tag="VCI33_001334"
     CDS             1725..2384
                     /locus_tag="VCI33_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP2832254.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            2406..4688
                     /locus_tag="VCI33_001335"
     CDS             2406..4688
                     /locus_tag="VCI33_001335"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEP2832255.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKI
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDHYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYSHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRASKAALFKGKANQALIDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(4768..4989)
                     /locus_tag="VCI33_001336"
     CDS             complement(4768..4989)
                     /locus_tag="VCI33_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2832256.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            5159..5533
                     /locus_tag="VCI33_001337"
     CDS             5159..5533
                     /locus_tag="VCI33_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2832257.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELEMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            5726..6025
                     /locus_tag="VCI33_001338"
     CDS             5726..6025
                     /locus_tag="VCI33_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEP2832258.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(6082..6810)
                     /locus_tag="VCI33_001339"
     CDS             complement(6082..6810)
                     /locus_tag="VCI33_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP2832259.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTEGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKRFITLIVCLTVETLLFVGGAYF
                     LQLTVWKYLLIPVFLLVLLGTLYWYYQNICYLCPSCHQTFEAKWTEFALAGHTPRTRK
                     LTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(6963..7550)
                     /locus_tag="VCI33_001340"
     CDS             complement(6963..7550)
                     /locus_tag="VCI33_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEP2832260.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            complement(7599..8129)
                     /locus_tag="VCI33_001341"
     CDS             complement(7599..8129)
                     /locus_tag="VCI33_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEP2832261.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            complement(8333..8824)
                     /locus_tag="VCI33_001342"
     CDS             complement(8333..8824)
                     /locus_tag="VCI33_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832262.1"
                     /translation="MKKKIYIGLIVIVGVFVVFLSGKQITQKETSNKPVVTLTVYTIS
                     SSDTQKWSKVKQFETKEAIYLVKVKETTSSEEVFSNIIADGMAVGFGVSEEDVKQFND
                     NLCSPVEDAKNNKLIGIEFINFSSVDKGFVVANFDYGKKAFNSQKTENKKLYKKLYEA
                     YKG"
     gene            complement(8821..>9125)
                     /locus_tag="VCI33_001343"
     CDS             complement(8821..>9125)
                     /locus_tag="VCI33_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP2832263.1"
                     /translation="ISAVLEKVGLSSGYLNQKIYELSGGQAQRVAIARMLMKPRKIIL
                     ADEPTGALDGEIGKEIINLLLSETAENKYVIIATHDPAVYNKVDFIIDMKDIGSKK"
BASE COUNT         2892 a         1838 c         1550 g         2845 t
ORIGIN      
        1 aggaccataa aaggccttat ctttatcact ttatttcata gtattataac attttttatt
       61 agctctcacg atcaacagat caatcttaac atgacaaagc ctatattctg atgttaatcc
      121 aattgtcatg tatctaaacg tgcttaatcc aattgtacct actaaaaata aggccacatt
      181 ttctctttca cgaatggctt ttgcgagtca tttttattga catgtacgaa tagcaaacta
      241 gaattatttc aaagcgctat agtaatggct ttacccacag ctaatcccgg ctcataggaa
      301 ctatctacag ctgttaagat acgtttgtat ttaagtataa gactaaactc cttttcttgt
      361 ccttaagaca aaacctgtct tgtctttgtt tttaataagt aaagcaaggt acaaatcagt
      421 ggaatcacag cagctaacca gaataagtgc ctaaaggaag ccacaccact accaagtgcc
      481 aaatctttaa tcaagcccaa gaggtaaggc ccagcaccta aacctaaatc aagaccaatc
      541 atataagtag acatagccgt attaaagcgg tgctcatcca ctccttgtat ggaggcggct
      601 tgaccacaag acataaaagt gccataacca aagccaatca aggctcctga aagcaaatag
      661 cttcctccag acgatacact acctagcaag aagagtccca ttgctaaaaa aagataactt
      721 gggtacaaga cccatttatc gcctttagca tccattaaac gtcccattgc tggacgagtg
      781 atggtaatga ttaaggcata gaccacaaag aaataagcgc ctactgttgt caaatgaatt
      841 tcagacgtgt ataatttttg aaatcctaaa actgaagcat aggcaatccc cattaaaaag
      901 gcgattgcag tgataaacaa ggcttttttc tcaatgaaac tatccacagt ccacgatttg
      961 gttttagcca attgctccgc atttaaagac atgtttttga ctggaaaagc aaaagcacca
     1021 acaaccacac acccaatcaa gacgctacat aagacaataa ccatgcgaaa atcaatatga
     1081 agattatcca acataaaggt tcctacaaaa gggccaatag ctgctgctag actggttgac
     1141 aagccataaa aattaattcc ttctcctctt ttacgagctg gaatataggc tgttacaatg
     1201 gtatttgtcg cagttgatac tacaccataa ccaaatccgt tcaagaaacg aaccaagtac
     1261 atcatgctaa tcgttggcat ataaaagtag gctaaggtcg ttaagaggta aaaaatagca
     1321 cctcctctta aaaccaaacg acgaccaaac acctccaact gctttccaaa aatcaaacgg
     1381 gctaataaag tccctagaat ataaatccct gttgccaatc ctgcctggct agtctgagct
     1441 cctagctctc tagtcgccac aaaggcaata ataaccgtaa aaagatagta gaccatatag
     1501 actataaagt ttattactgt gatggccaca aaatgtttgt taaacaattt ttcttccatt
     1561 gaaaatgaac tcctctctat tttcataaac attttacaaa aaaagcagaa tataatatat
     1621 ttcaatggta taatataagt gttactatca aagggcatta gcaaatgtta atttctcttg
     1681 aagatacaaa ttatacattg gtaaaagaat atttggagaa aagcgtgaat catcacattt
     1741 tgcaacggta tattgataat cataactttc ctatcattga aaaaagttat cacaagtact
     1801 tgacttttga aagtctcgaa gaggacttca cttatatttt aaaagacggc attgtgaaac
     1861 agagtgttct ttcaaaatat ggaatggaat ttaatttgag atatgttaca ggacttgaaa
     1921 ttacttctgt tctcaacact ggttattcaa aagatatggg agagccttat aatgttcgca
     1981 tcgagtcaga gaaagctagt ttttacaagg ttcgccgctc agcattttta aaagatataa
     2041 acgaagatat tgaactacaa ggatatgtga aggactttta ccataatcgt cttcaaaaat
     2101 ccatgaaaaa aatgcaatgt atgttaacta acggccgtat cggtgccatt tctactcaaa
     2161 tatatgactt aatgacccta tttggcgaag aactaccaaa tggtcaaatt ttaattaatt
     2221 ttgtcattac taatgaggaa ctcggtaaat tctgtggcat ttctaccgct agcagcgtca
     2281 gccgtattct aaaacagttg aaagaaaaaa acatcattcg cattgataaa caacatatta
     2341 tcattaccaa tcttgataaa ttaaaagata atatcgtttt ctaagccact agaaaggaaa
     2401 ctcctatgcg ctataatcaa ttttcctata ttccaactag tctagaaaga gcggctgaag
     2461 aattaaaaga attgggcttt gatctggatc tgcaaaaaac tgccaaagct aacctagaaa
     2521 gctttttacg taaaatcttc ttccactacc ctgatagtga ttatccttta agtcatctaa
     2581 tcgccaaaaa tgacatggac gcattgagct tttttcaatc agagcaagag ttatccaaag
     2641 aggtttttga tttactggcc ctgcaagtcc ttggctttat tcctggagtt gattttacag
     2701 aggccgatgc tttccttgat aaactggcct ttcctatcca ctttgacgaa acagaaatta
     2761 tcaagcatat ccaccactta ttagcgaccc gttgtaagtc tggcatgacc ctgattgatg
     2821 atttggtcag ccaaggaatg cttactatgg acaatgatta ccatttcttc aacggtaaat
     2881 cattagctac ctttgacacg tcacagctga ttcgagaagt tgtttacgtt gaagcacctc
     2941 ttgataccga tcaagatggc caacttgatc ttattaaggt taatatcatt cgtcctcaat
     3001 ctcaaaaacc attaccaact ttgatgacac ctagtccata ccatcaaggt atcaatgaag
     3061 tagccaacga caaaaaactt tatcgtatgg aaaaagagtt agtcgttaaa aaaaggagac
     3121 aaatcactgt cgaagacagg gattttattc ctcttgagac acagccttgc aaacttccta
     3181 taggtcaaaa tctagaaagc tttagctaca tcaactccta tagcctcaac gattatttcc
     3241 tcgcacgtgg gtttgctaat atctatgttt caggtgtggg aactgctggt tccactggct
     3301 ttatgacgag tggagactat gctcaaattg aaagctttaa agccgttatt gattggctaa
     3361 atggaagagc cacagcctac accagccact caaaaaccca ccaagtgaga gctgattggg
     3421 caaatggtct agtttgtacg actggaaagt cctacttggg aactatgtct actggtttag
     3481 ccactacagg cgtagacgga ttagccatga ttattgcaga atcagctatt tcttcttggt
     3541 acaactacta ccgcgaaaat ggcttagttt gtagtcctgg tggctatcct ggagaggatc
     3601 tagatgtcct aactgagctc acctactcgc gtaatttatt ggctggcgat tatcttcgcc
     3661 ataatgatca ttatcaagaa ttgctaaacc aacagtctca ggcgttagac cgacaatctg
     3721 gtgattataa tcaattttgg cacgaccgca attacttaaa aaatgctcat caaataaagt
     3781 gtgatgtcgt ttatagtcat ggcttacaag actggaatgt caaaccaaga caggtctatg
     3841 agattttcaa tgcgcttcct tccacaatca acaagcacct ctttttacat caaggtgagc
     3901 atgtttacat gcataattgg caatccattg attttcgcga aagcatgaat gcccttttat
     3961 gtcaaaaact cttaggccta gctaatgatt ttagccttcc tgaaatgatc tggcaggata
     4021 acacctgccc acaaaactgg caggagcgta aagtctttgg aacctcaacc atcaaagagc
     4081 ttgacctcgg tcaggaactg cttttaattg ataatcacta tggtgaagat gaatttaaag
     4141 cttacggtaa agatttccgt gcgtccaaag cagctctctt taaaggcaag gccaatcaag
     4201 ccctgattga tattttactg gaagaggact tacctattaa cggtgaaatt gtcctacagt
     4261 taaaagtgaa atcaagtgaa aataaaggac ttctatctgc acaaatcctt gactatggta
     4321 agaaaaaacg cttgggcgat cttcctattg ctctcaccca atcaagtatt gacaatgggc
     4381 aaaatttctc tagagaaccc cttaaagagc taccatttag agaagattct taccgtgtta
     4441 tttcgaaagg tttcatgaat ttacaaaatc gcaataattt gtcgtcaatt gaaactattc
     4501 ctaataataa atggatgact gttagacttc ctttgcaacc aactatctat catctagaaa
     4561 aaggagatac ccttcgagtc atcttataca caaccgattt tgaacatact gtccgtgaca
     4621 acagcaacta tgctttaact atcgatctca gtcagtctca attgattgtg ccaatagcat
     4681 caaattaaca aataaaagtg cttagcaata taactaagca cttttttgtt aattggtctt
     4741 actaatagca aacagaaaac gatcgtctta gttcataaat tccgaatcta ttcctaaaaa
     4801 aatatcatca acggaaaccg ctaatgcttg tgcaatacct tttagcttct catattttgc
     4861 tcgccttagc tttttagtat ctttttcata cctagcaatc gttctgacag aaatgcctgt
     4921 taagtgagac aattcctgct gtgtcatttt ctttgttgtt cttaactctt ttaaagttga
     4981 tttggtcaca attttccctc cacactaaaa tgagattagt taatcatgtt aagtttatta
     5041 taaacttcgg tttttatgaa gtcaagtttt tagagagttt ttagaccatc ttttacgata
     5101 ccttttgctt taacctcttt tatggtatca tattttatat aaagaaaagg agaaaaatat
     5161 gtccgccaag aaaacttttt ttgcaagtaa tttaaagtac cttagattaa aaaagaacat
     5221 ggaacaatta gaacttgcta atctcctagg acgaaaaagc tcttcttcaa tcagtgagtg
     5281 ggaaaaagga aaatatactc caaagtctgg tctattaagt gatattgctg ctatctttga
     5341 tgttagttta accgccctaa tggaagaaga tttaacctta gaaagtaagg gagtctctta
     5401 tcagttgcca caacgagagt tagagatgat ttcagaacaa ttatccgaaa ataactacac
     5461 taagtgggtt gacttcgcac aaattctttt agaacaacaa gctaaagaag atcgtgaatt
     5521 agataaccac taagtcggac tagtctatga caaaaataat ccatttctag acttattctg
     5581 tcattagcca gacccttgct gctaatccat cttatccata gcgactttct tactgataca
     5641 ggctagtcta ctaactgaca ttgtttttat tgcctctttt tatatgttat aatagttagt
     5701 attaaattaa ggagagtata gtcttatgat gaacatgcaa aacatgatga agcaagcaca
     5761 aaagcttcag aaacaaatgg agcaaaagca ggcggatttg gctgctatgc aatttactgg
     5821 aaaatcagct caagaccttg tcactgccac tttcacgggt gataaaaaat tggttgggat
     5881 tgacttcaaa gaagctgttg tagatcctga agatgtagaa acgcttcaag acatgaccac
     5941 tcaagccatt aatgatgccc ttacgcaaat tgatgaagcg actaagaaaa cattaggcgc
     6001 ttttgctggt aaattaccat tctaaaaaaa caaaaactag cttgagaagg atctcaggct
     6061 agttttttta tgagttagtg cttatcctct tgagacagtg tcctcttttg ccagttccaa
     6121 gcaatacgat ttttgatggc aatgggagca ggttaacttt ctggttcgtg gtgtatggcc
     6181 tgcaagggca aattctgtcc attttgcttc aaatgtttga tgacaagaag gacaaagata
     6241 acagatattt tggtaatacc agtataaggt tcctagtaag actaaaagga acaccgggat
     6301 taataaatat ttccagactg tcaattggag aaaataggct ccacccacaa aaagtagagt
     6361 ttccactgtc aaacagacaa ttaaagtaat aaagcgtttt ttccaagctt tttgattttt
     6421 catgctaagc gaaatgtcca tcagaaaatc tagtgactgg gggtcttgtt ttcgaagtct
     6481 gtcaagtatc ttgacagcta tatcaacttg ttgctctttt gcggccaagt cttccttggc
     6541 agtcgcaata tgatcaacta gaagcaattc cagcacctgg gctgcatttt cctctgctaa
     6601 gactttgcga atctgctcaa tggaaaatcc caaatcgcgc agaaagcaaa tcatgcgtaa
     6661 ttgttctaaa tcagaatccg tatagagtcg ccgtccacct tctgtcaaag ctgtcggaat
     6721 caaaatccca cgttggtcat agtattggac agttcgaata gaaacgccag ctaagttggc
     6781 caattctcca gtcgaataga attgagacat ttccacacct tctttctatc atttagtgat
     6841 caaaaacgat tcgtgtgcac tcgactccct cttgatatgc ccctatctta tcacatgacc
     6901 tagcgttaca agcaagagct aaccctagga atttttgtta ttttttagaa atagcagagc
     6961 ccttagtcaa aaaagtctcc caaagctgcg aaagggttat ctgtggttac ctcttcttgc
     7021 tggctgagat atgctttgtt tgtatctgtt tctaaaaatg acttatagat tgctgctgtc
     7081 attctggcat cttctaagct attatgacct cgacccttaa tgcctaaaaa ggttgccact
     7141 gtttgcagac gaaggttagc aatcccattt aagtctgcgc tacgtctatc gtaagcttca
     7201 tcaaagaggt caatctggta ttggtccctt aaatctaagc cattttcagc tagaataggc
     7261 aggtcagatt tttgagcatt ataaccaatc agtggtaact ctcccacaaa atttttaaac
     7321 gcagccataa cctcctctac ttttggtgcc gcagcaatct tgtcagatgt gattcctgtt
     7381 aggccattga taaaactctg taagggaaca tctgtgtaaa cataggtgtc aaagctgtca
     7441 acttctttat ggtggtcata tttgactgct gatacttgaa tgacatgact aacgtcattg
     7501 acagtgttaa attctaaatc aaaggcaata taagtatcta agtgtttcat gttttcctcc
     7561 aaaaaagacc ctgatagggc cttcgtcatt ttttgtgatt atttcgcaaa caagcgggtt
     7621 aaaaagcctt ttttagggcc aacttcagcc tgaacttctt cgacttgtaa gcgagcttgg
     7681 ttggcttctg ctttagcttc ttccaattct agttttaggg tttctttatc agccatggct
     7741 ttagcagtta attgttgctg ctgatccaat tgtttatctt tttcagcgat ctgcacgtct
     7801 ttaacacgca tttgctcatc ttttgaggca agttgagcat ctttggcttt gagttgctca
     7861 taaaggcgcg tgatttcagt gttcttctca tccacaagaa tctctaaaag ctcacgttgt
     7921 ttcgtttctt cactgatagg ctcatcgtca aaaattgttt ttttatagat ctcttccagc
     7981 ttaacaagac cactgcgctt aaccacagta acgccttttt catttttgtc aagatcttct
     8041 tcgggtaaag acttaacacg attattgacg gcctgacgac tcactcccaa aatatcagct
     8101 agttcactaa ctgtcttttc aattcccata ttttcctctt tatcactatc actgaatatt
     8161 cttattaaaa atagtaccat aataggagta ttctgtcaat ttgacctcat ttcagtcgtg
     8221 ctaaaggcat caattatttc ttgaaaacat tgttagatga atagaaaacg gtatgccatg
     8281 ttagttcatt tattattcag aagtggtatc actctcaaaa aactataaat agctatcctt
     8341 tatacgcttc atagagcttt ttatataatt ttttattttc tgttttctga gaattaaacg
     8401 cttttttgcc gtagtcaaaa tttgcaacca caaacccctt atcaacacta gaaaaattga
     8461 tgaattctat tccaattaat ttattattct tagcatcttc taccgggcta cataaattat
     8521 cgttaaactg tttaacgtct tcttcactaa ctccaaaacc aactgccatt ccatctgcaa
     8581 tgatgtttga aaagacttct tctgaagagg tagtttcttt tacctttact aggtatattg
     8641 cttctttcgt ttcaaactgc tttactttac tccatttttg ggtatcacta gaggagatgg
     8701 tataaactgt tagggtcacc acaggcttat ttgaagtttc tttttgtgtg atttgttttc
     8761 cagacaagaa cactacaaaa actccaacaa tgacaatcaa gccaatataa attttctttt
     8821 tcatttctta ctccctatat ctttcatatc aattataaaa tcaaccttat tatatacagc
     8881 tggatcatga gttgctataa taacgtattt attttcggct gtttcgctca acaacaaatt
     8941 aataatttct tttccaattt caccatccaa agctcctgtt ggctcatctg ctaaaattat
     9001 tttacgtggc ttcattaaca ttcttgcaat agctacacgc tgagcctgcc ctccagacaa
     9061 ttcatatatt ttttgattta aatagccact agagagccct actttttcaa gtacagcaga
     9121 tattt
//
LOCUS       DATUYR010000053         9105 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.053, whole genome shotgun sequence.
ACCESSION   DATUYR010000053 DATUYR010000000
VERSION     DATUYR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.053"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..508
                     /locus_tag="VCI33_001344"
     CDS             <1..508
                     /locus_tag="VCI33_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832264.1"
                     /translation="NITHGFIVNELNDLVSGIRDEMDTYYPIVPANQTMKENIQSLFD
                     DIVKSRKSNTLLYFNCSDNMVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSLTIQS
                     DIIIIEESNQVVEKVHSISYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLPIDWK
                     LCYEDFIN"
     gene            489..1094
                     /locus_tag="VCI33_001345"
     CDS             489..1094
                     /locus_tag="VCI33_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2832265.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDITLLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(1646..3226)
                     /locus_tag="VCI33_001346"
     CDS             complement(1646..3226)
                     /locus_tag="VCI33_001346"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP2832266.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLFAVCDNLEEHFNTFTDAQKIFENAYQDEGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGWEDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDSHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(3239..4426)
                     /locus_tag="VCI33_001347"
     CDS             complement(3239..4426)
                     /locus_tag="VCI33_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP2832267.1"
                     /translation="MTKSKRPQYRFDSFEGEWEEKKLGEISNIVRGASPRPIQDPKWF
                     DAKSDIGWLRISDVTEQEGRITYLQQRISELGQEKTRVLKDPHLLLSIAATVGKPVIN
                     YVKTGVHDGFLVFLDPKFNREFMFQWLDMFRPYWNKYGQPGSQVNLNSEIIRNQVINL
                     PSLPEQEAIGELFQTVDQLIQLQRQKLAILKEQKQTFLRKMFPAQGQKVPEIRLQGFD
                     GEWEEKELGDIVQITMGQSPSSQNYTTNPSDYILVQGNADIKNGYVFPRVWTTQITKQ
                     ADKGDIILSVRAPVGDVGKTNYHVIIGRGVAAIKGNEFIFQILKYLKEIGYWKRISTG
                     STFDSISSSDIKYAKIQIPSLSEQEAIGNFFQTLDQQIAQSEEKLTELKALKQTLLNR
                     LFV"
     gene            complement(4439..7417)
                     /locus_tag="VCI33_001348"
     CDS             complement(4439..7417)
                     /locus_tag="VCI33_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEP2832268.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTASAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVATTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRYLTNTFRNSRWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLDSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLMKKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDTALSLYSNGGQSEVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLYASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIAYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGISTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            7698..8840
                     /locus_tag="VCI33_001349"
     CDS             7698..8840
                     /locus_tag="VCI33_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEP2832269.1"
                     /translation="MTINRVLKDDNLLQYTEREFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFYQKTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILVANLMLLIAQFNK"
BASE COUNT         2765 a         1567 c         1747 g         3026 t
ORIGIN      
        1 taatataaca catggtttta ttgttaatga gttgaatgat ttagtttctg gtatcagaga
       61 tgaaatggat acttactacc caatagttcc cgctaatcaa acaatgaaag aaaacataca
      121 gtctcttttt gatgatattg ttaaaagtag aaaaagtaat accctattgt attttaactg
      181 ttcagataat atggtagtgc catccccata tggagatatt gtttataggt ttataaaaga
      241 gctaataaac aatgctataa aatatggaga tggaaaagac attcgtttat ctttaacgat
      301 tcaatctgat attattatca ttgaggagag caatcaagta gtggaaaaag tacactctat
      361 aagttatggc agaggattga aatcatttca agaaacacta gcagcttttg atggagatct
      421 agaactacaa atggatacta aacaatttac tatcagaata ttacttccca tagattggaa
      481 attgtgttat gaagatttta ttaattgatg accacagact atttgcaaag agtatccaac
      541 tgttattcca acagtatgat gaagtagatg tcatagatac gattacttct cattttaacg
      601 atgtgacgat tgatttgtct aagtacgata ttacattgct tgatattaat ttaacaaata
      661 tttctaaaga aaatggattg gagatagcaa aagaactcat acagtctact cctcatttga
      721 aagttgttat gttgacagga tatgttaaat caatctatag agagagggca aaaaaagtag
      781 gagcatacgg ttttgtagat aaaaatatag accctaaaca actcatttct atcttaaaaa
      841 aagtggattc tggaaaaaag tattttgaac aaatcgaatc tcaggattat gttgaatctt
      901 taactgacca ggaaatagct atcttaaatt tgagtaagaa aggattctct ataaaagaaa
      961 tagaggaaac gttacaaatt agtaggagaa cagtatttaa tcatttgacc cacatctact
     1021 caaagctttt ggtaaataat aagcaagagg ctatctacaa agccgaacaa ttagggtatt
     1081 ttatggattt ctgagttgca gataatcaaa cgacataagt tttacttgtg tcgttgctag
     1141 ggatttcctg ttaaataccc agatttaaac tcattgtttt tgagttcttt ttgattgaaa
     1201 ttgattgaga taacgctttt ataaaaagta gggcaatgcc tgatatgaaa agctttttaa
     1261 atttagtcaa acaaaagttg tttaaaccag gtctaaaaaa actcgtaaag cttcacaact
     1321 cccagaacgt taatatatgc ttatatatca acgattggaa ctactttatg gttcgcacca
     1381 tggtttttgt ggaaggatca aaagttgtcc tgaaaatttc tcttaaccgt gtttaagctt
     1441 tctaaacgtt tgagaaatca ttgtaacttc aaataactat tttaaataaa acaaagaaac
     1501 ggacaaagtt agatgagatg attctatttt gatttatagg tgaagaagga gaatcttgtt
     1561 tgataagtac tcaataaagg ttcactaccc aattttaaat aaaacgccac agtagtaatc
     1621 atctactgtg gtgttttttg gtcttttagt ttctaaaagt atccaattcg tcttgtgctt
     1681 ctttggttgt tccgactagt tgtttcatga gttgatctag tttggcattt gttttggcaa
     1741 tttcttgatc aatgtcactg agctgtttag ctaattctgg cagtggtttg acggggactt
     1801 cctcaaaggt atcgacatag cgtgggatat ttaagttata gtcattttca atgatttcat
     1861 caaatgaggc taaatatgaa aacttatcag aattatcacg actcttataa gcatcaagga
     1921 tctttttgat atggctatct gtcattgtgt tttgattttt gcctttatca aactcttttg
     1981 aagcatcgat aaagaagaca tctttgttgg ttctgttctt ctttaatatg ataatagtcg
     2041 tagggatgct tgtattataa aagatatttg atgggagtcc gataatggta tcgatagccc
     2101 cttgttcgag tagtttttgg cgaatcttgc cttctgcagc accacggaaa aggacaccgt
     2161 gtggtaagac aatcgccata gttcctgtat tttttaaatg atagaaacca tgaagtaaaa
     2221 aggcaaagtc agcttttgat tttggtgcta atacaccata acttgaaaac cgtggatctg
     2281 ttaagaaacc agcagtcgct gaccatttca aagaataagg tgggttcatt aacactccat
     2341 caaagtttgt tggttcatct gttggccagt ctgcatccaa ggtatcagcg ttacttaagt
     2401 gttggttttc gatggcaacg ccatgaagca tcatgttcat acgagccaag ttataagtag
     2461 aagtattgat ttcttgacca tagtaagaga cggtatcgga ttggttacta tatttcttag
     2521 catttaaaag gagtgatcca gatcccatag cagggtcata aagggtcatt cctttttgat
     2581 cttcccaacc taaaaagaca atctgagtca tcaagtgaga gacagcttgt ggggtataga
     2641 actcacctgc tttttttcct gactcactgg caaattcacc aatcaagtat tcataagcat
     2701 cacctaaagt atccccatct actgcctcaa agtcaatttc atttaatgtt ttcatgacat
     2761 tagaaatggt ttgattttgt ttttgaggag tagagcctaa ttttttagag tagaggtcaa
     2821 tatcttcaaa taagttttca aagtcttcac cactttgttc aatatcgcga aaaccttgag
     2881 ctaatgattc taattggaaa gtattgtgat aaacatcttg gatgagtttt tcaaaggtca
     2941 gtgtaggttc gataaagtaa cctaaatcac cagtaacaac agaaatgagg tcatctttta
     3001 ggccttcatc ttgataggca ttttcaaaga ttttttgagc atctgtaaag gtattaaagt
     3061 gttcttctaa attatcacaa acagcaaaga gtaatttatc agataaatgt ttgtaaaaga
     3121 tgagtccaag aagatagttt ttatagtcat tggcatccat ttggccacgt aattgatcgg
     3181 ccgaatgcca tagtgcttgc cgtagtgaag ttgttttctc tgccatgtgc gtcctttctt
     3241 atacgaataa acgattgagt aatgtttgtt tgagtgcttt gagttctgtt agtttttctt
     3301 cagattgagc aatttgttgg tctaaagttt ggaagaaatt accaatggct tcttgttctg
     3361 agagggaagg gatttgaatt ttagcatatt tgatatcact agagctaatg ctatcaaaag
     3421 ttgaacctgt actaattctt ttccaataac caatttcttt caagtatttt aatatttgaa
     3481 agataaattc attacctttt atagcagcaa ctccacgccc aataataaca tgataatttg
     3541 tctttccaac atcaccaact ggagccctaa cactaagaat aatatcacct ttgtcagctt
     3601 gttttgtaat ttgtgttgtc cagacacgcg gaaaaacata tccatttttt atatcggcat
     3661 ttccttgtac tagaatataa tcagaagggt ttgtagtata attttgactt gatggagatt
     3721 ggcccattgt aatttgaaca atatcaccta gttccttctc ttcccactca ccatcaaaac
     3781 cttgtaaacg gatttcaggg actttttgtc cttgagctgg gaacatcttt ctgaggaagg
     3841 tttgcttttg ttctttaagg attgctagct tttgcctctg taactgaatt aactgatcaa
     3901 ccgtttggaa gagttcgcca atggcttctt gttcagggag ggaggggaga ttgataactt
     3961 gatttcgaat tatttcagaa ttcagattca cttggcttcc tggttgacca tatttattcc
     4021 aatatggtct gaacatatct aaccactgaa acataaattc acggttgaat ttaggatcta
     4081 aaaacacaag aaatccatcg tgtactccag ttttaacata attgataact ggtttaccta
     4141 ctgtagctgc aatacttaat agtaaatgtg gatcctttaa tactctagtc ttttcttgac
     4201 caagctcaga tattctttgt tgtaaataag ttatacgacc ctcttgttca gtaacatcag
     4261 aaattcttaa ccaacctata tcacttttgg catcaaacca ttttggatct tgtatcggtc
     4321 taggactggc accacgaact atattagaaa tttctcccag ctttttctct tcccactccc
     4381 cttcaaaact gtcaaagcga tattgaggcc gtttagattt tgtcatctct ttcctttcct
     4441 agtgcccttt taagggttgg atttgatctc taatgaattg aactaactcc cgttcaaatt
     4501 ggcttttgta tttgagttta ttcatggccg agtcaccttg tttttcttta taggcttggt
     4561 agtctaggga ttcttttaag gttgatatgc ccacttgttt ggtagagtta gcactatgat
     4621 aattagtcgc cacaaaggct aatttgtcag gatctgcctt ccactggtcc gcaaagtgtt
     4681 tgatggccct ttggatctta tcaccaatga ggttatccaa ctcataagca atgcttttcc
     4741 cttcataggc tttgggttct ttttggatat cttgccataa ctcagctagt gagtcagcca
     4801 tagcagggtt tgattttgcc aaatcttgga tgtattgatc catgggattg tcattgagcc
     4861 tttcttgaag agcttcttgt tcttgatcga caaaggcttg aattaaggtc aagatatagt
     4921 gatagttgat atcatccatc tgaacacttt ctagctcata gtcgatattg atctctggga
     4981 tggcttcgtc atcttctctt cgctttcgta tttctgcaat gacattttgg taagtgcctt
     5041 tataagtatc aatcacttca tctgataacc caacctcact caatagtgtt tcattaaagt
     5101 cactatagac ctgtacagat gcatagagct tatcaaacgc ttgaaaggct ttggcgtatt
     5161 gtttgagaaa agctgtctgt gcgctctcaa tagttgggaa agcgtctgga tcaggaacta
     5221 tattttttaa tactgttact ttttcaaaga atcttgcttt ttcttcttcc caagaaggcg
     5281 ctaaaacttc actttgacca ccatttgagt ataggctaag agccgtatca acagcttctt
     5341 taaaacgaag tggtgtttgg aaagtcacaa cctgaccgta atgttttcta ctttcaaaga
     5401 tacggttggt tcgtgagaaa gcttgaataa tgtgctgagg tttcataggc tggcgatcga
     5461 tgaagatggt tgataaacaa ggcgcatcaa agcctgttaa gagacggtca accacaatca
     5521 cgaggtctaa ttgttcatga cggtctttga atttatcttt tttacgggct aagcgatcat
     5581 ttaaatctct gttatagcct tgtaagttat cgatggtaaa gttagtgcca aagaggtgat
     5641 tatagtcttc tagatttttg gtcattttat cttgtcggct gatagacgca ttgtcatttt
     5701 cagtgataga ataagtaata gctacctttg gaaagtctgg tagtttttct ttgaccttct
     5761 tagaaatgac taaatccgtt tcaccagcct taactttctt catcagatca taataggctt
     5821 gggcacgagc aatactctta acggttagta aaccttcgaa tgtctgacca attccattat
     5881 taaaaccaag tttcttgcgt gattgattaa tgatactatc taagacagct aacatatgtt
     5941 cttcatgatc ataagcttct tccggaatac tgtcttcagg catatcagga atggttgtct
     6001 tatactcaac ttgaaagcct agaaccgctt tatcatgaat ggcttcttta acggtgtatt
     6061 gatggagaca tttgccgtat tgctgttccg ttgtttgggc taaatcacct aattgagcac
     6121 gcttattttc cacaaagata ggagtgccgg taaagccata ccagcgactg ttacgaaaag
     6181 tattcgtgag ataacgttga cgttccggtg tgacagcacg gtggcattcg tccacaacaa
     6241 agacaacatt taagtgtgca agacgctctt ttagtttctt gaattttgga gtgtcataac
     6301 tttccatctg actgatcatg gcattgagtt tttgaatggt ggtggccaca acacgacgat
     6361 cactggattc taaatttttg atgagttgtc tcgtatcttc tgtctcatcg acatcaaaga
     6421 tatcattttg cgcataggat tgaaaagcag aagcggtctg attatctaaa tcctttcggt
     6481 caatgacaaa gatggatttt tcaacggccg gtatttgaag gatattgcga gccactttat
     6541 aggaagttaa ggtttttcca gaacctgttg tgtgccagat atagccagac ttgcggtgac
     6601 gactcgcttc agcgacggct tcgattgcat gaatctgata aggacgaagg agaattagtg
     6661 cttttttatc atcatcaatg acagagtaag tcatgaccat ttggtgagca cgagggatag
     6721 ataggacttc tttggcaaag gcaaagagat ctttttgtgg tttgttgttt tgatctaccc
     6781 attgggtcaa gaagttaggg tttaatttat tttctttagc tgcagcgata taccttgtat
     6841 ctgttttatt tgagaccaca aacatttgta aggttgaaaa gatacctcta aactggcctt
     6901 ctttatcata ctttttgact tggttaaaag cttcaatgca ttgatgattc tgagatttta
     6961 attcaatttg gatcataggt aagccattga tgagaagtgt cacatcacct cgtctatcac
     7021 gagaaccaga gaaagcaact tgattggcaa tctcataaac agaagttcca ccagccacat
     7081 tgtctgcttt aacgacttct aaacgaatcg ttcctagttt tgcatcttcg cgttgcactt
     7141 gtactttagc aatgccgttt tcaccagcta accatttagc cgcttcatag taattgacaa
     7201 agttaagttg attcttaatc tgctcttttt cactagctgt taaaggctcc tcattcagat
     7261 attgcgtgtt attttgcgct aagatcttaa agaaattatc ccataaagca tcttcagttt
     7321 ttaattcttt acgatatgtc cattgacttt cacctgtttc taagagatgg atcaactctt
     7381 tttcaagttc aagttccgtt ttagtttgat ttcccatttt taatccccat tcgacatgat
     7441 atagtaatat tatatcattg gcggatcctt tttagaatgg caaagctatt gaaaccttcc
     7501 aaacagtctt tttttgatta gttgaatgct taagatccta gcaactaaac aaagctatga
     7561 ctatgtaaat gcaatcgaga aagttcttgt atttttgagt ttgcttgaca ataatttaag
     7621 gcgaagaaag taataagaca acactagtaa aaaggtcgag ttctcaaaat agataaaaaa
     7681 cttaagacag atctagcttg actattaata gggttttaaa ggacgataat ctgctacaat
     7741 atacagaaag ggaatttatt gaagatatga aaattttggt agccatagat tcttttaagg
     7801 gatcggtaac gtctccagaa cttaacacta gtgtagctca agcccttctt tctgtcgata
     7861 agcagttagt tattgaaaca agagctatcg ctgatggagg agaaggaagt ttagcagccc
     7921 tctcacaaac cgtggcgggg aggtggcatc aagtaaaaac cattgatcta ttaagacgcc
     7981 cgattaaagt cgcttattat cgtcacgcaa agcaagcttt tattgaatct gcgagtatta
     8041 ttggtattga taagatcacc cccaattcag taacctatgc acaagccact tcctatggtt
     8101 tgggtttggc agttaaagat gccattcaaa aaggagctac tcaaatcgaa atcatgctag
     8161 gaggaacagg cacttctgat ggtggcaaag ggtttttgga aagtttaaac tatgatttca
     8221 tgacaggaag atcttatctt gatactttag catcacctgt tacgctatta gggctgactg
     8281 atgtgaccaa tccatatcat ggccctcaag gatttgctgc ggtctttggc cctcaaaaag
     8341 gtgggagtct tagtcaaatt gaagaaactg atcaaattgc aagtaacttt gctaaaaaag
     8401 ttttttacca aaaaaccatt gatttacaga caataccagg aagtggagca gctggaggat
     8461 taggaggtgc cattgtccta ttaggaggca ccttaactag tggttttagc aggattgcag
     8521 agctactaaa tctggataat tcccttcagt cctgtgattt agttattact ggagaaggat
     8581 gcctggatac acaatcacaa tctggcaagg tacctgttgc gattgcaaga atggctaaaa
     8641 aatatcaagt tccaacgatt gctctatgtg gttctgtaaa gattgagaca ggactagcag
     8701 cagaagactt tttggctgtt ttttcaatac aacagcagcc aatttcctta gaagcagcga
     8761 tagataaaac aacaacactt tccaatatca aaatattagt agctaacctt atgctattga
     8821 tagcccagtt taataagtaa actcatctat tttcgattgt gtagcattca tttggcggtt
     8881 aaaagtaacc tatgaagaag gagacgtacg agctaactca ttagcatagt atgaaaaaca
     8941 gaaacatttt gtaaaaaaaa gagttgacaa tgacagtaaa gatatcgtat actaataaag
     9001 ctgtcagaga ggttgctgta acacacttat tgaaaaaaga tgaaaaagta gttgacaggc
     9061 tatgagaaac ctgatagaat aaagaagttg tctcttagga gacgt
//
LOCUS       DATUYR010000054         9088 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.054, whole genome shotgun sequence.
ACCESSION   DATUYR010000054 DATUYR010000000
VERSION     DATUYR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.054"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(77..1390)
                     /locus_tag="VCI33_001350"
     CDS             complement(77..1390)
                     /locus_tag="VCI33_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEP2832270.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKADQKLLLACASSAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWSVLAGLILSLFAHAYKHLLEHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
     gene            complement(1365..2324)
                     /gene="nrdF"
                     /locus_tag="VCI33_001351"
     CDS             complement(1365..2324)
                     /gene="nrdF"
                     /locus_tag="VCI33_001351"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP2832271.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINNIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(2657..4816)
                     /gene="nrdE"
                     /locus_tag="VCI33_001352"
     CDS             complement(2657..4816)
                     /gene="nrdE"
                     /locus_tag="VCI33_001352"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP2832272.1"
                     /translation="MSLKDIGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(4836..5054)
                     /locus_tag="VCI33_001353"
     CDS             complement(4836..5054)
                     /locus_tag="VCI33_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEP2832273.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            5447..5710
                     /locus_tag="VCI33_001354"
     CDS             5447..5710
                     /locus_tag="VCI33_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEP2832274.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            5715..7448
                     /gene="ptsP"
                     /locus_tag="VCI33_001355"
     CDS             5715..7448
                     /gene="ptsP"
                     /locus_tag="VCI33_001355"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEP2832275.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            7633..9060
                     /locus_tag="VCI33_001356"
     CDS             7633..9060
                     /locus_tag="VCI33_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP2832276.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
BASE COUNT         2906 a         1635 c         1902 g         2645 t
ORIGIN      
        1 cacttaattt tgtgtctact ttagtatacc aagtccaatt catccgaagc tttttattaa
       61 aaaagtaatt aactaatcat ttaaggatga aacgataaaa ctgatagcta aaataaaaaa
      121 tgaggttagc taatgctaaa gggataatca ttagtaacga ttggcctgta atttcaacca
      181 gaaaaactgg tgccgccaag ggtatcttat tgatcacacc taaaaagatt gttgcaccaa
      241 ccataatggc taacgtattt gatagagaca agggaataag gtactgacta aggatagcta
      301 gtagtaagcc actagcacct ccaagcatca tagaaggagc aattttaccc ccttttgctc
      361 cagaagcaaa aactaaagag atagcgacag ctttggcaac aagtagccag ctaatatagc
      421 tcagatgtgg ttcttcatgg agaaaaaaga gtaatccagc tttgccattt cctaaaatct
      481 ctgggaagaa aatgctgagt cctgcgataa ggctaaaagc gatgagaact ttcggaataa
      541 accactgact tttggcatca gccttaggaa ggtgttccaa aagatgtttg taagcgtgag
      601 caaagaggga aaggataaga ccagccagga cggaccaaat aagggttcct agcgtccaat
      661 gttgctgagg cattaggtat tgaatctcat gtcggccttg taataaagca actgtttcca
      721 cagccacata gctcgttagg caggcagcgt atatattttt aagggaccaa cggttaagaa
      781 tagcttccaa gataaataaa atagtggcta agggagcatt gtagacagca ccaagggcag
      841 cgctagaagc gcaagctaat aaaagttttt gatcggcttt tgacaagttg catctttggc
      901 tccaaagaga cgtcaaggta acagcgactt cccgtgaagc tccttctcgg ccgacaggag
      961 ctcccataga tacggtcgtt aattggagcc aaccatgcca aaactgtgtc cagggactaa
     1021 atgagatgtc atcttgaatg atttgctgaa tagattgaat gtcctttcct tttttagcca
     1081 ataggtgcca cccaagacct gcaagaagtc cagcaaagag aagtgaaaga gctcgtcttt
     1141 gtggtggaac gctagcaatc atagtgctaa aagaaccttg actgaatcca aaactgaggg
     1201 actgaatcag gtgaatggct tttgttaaga taaaagcaac aaggccagct gttaaacctg
     1261 ttaaaaagag gtaaaaggtc aagagtaaaa agtgtcgttt cataagacta ttataacaaa
     1321 agttaagaaa agagactgat agattatcag tctcgtgagc actattagag tccataatta
     1381 taatcgtcat cagacatagc ttctacagaa ccaagaagat agccattccc tacctgtgag
     1441 aaaaagtcat ggtttgatgt tcctgttgaa ataccattca taacaattgg gttaacatcg
     1501 tttgcagtat ctgggaataa aggatcttgt cctaaattca taagagcttt gttagcattg
     1561 tagcgtaaaa aggtcataac ttcctcagtc caccctacgc catcataaag cgttttggtg
     1621 tatttttctt cattttcata cagctgataa aggaggtcat acatccaatc acgaaaattc
     1681 tcttgctcat cttctggtag ttcgttaaaa ccaagctgga atttgtaacc gatataagta
     1741 ccatgtacag attcatcacg aataattagt ttaatgattt cagcaacatt tgctagctta
     1801 ttatttccca aatagtaaag aggggtgaaa aagccagaat aaaaaaggaa agtttcgaga
     1861 tatgttgagg caactttctt ttgtaaggca tctccattag cgtaaatgtt attgataata
     1921 cgtgcttttt cttgaaggaa ctcattatta ttagtccact caaagatctc ttcaatttct
     1981 tttttggtgt ttaaggttga aaagatagac gagtatgatt tagcatgaac tgattccata
     2041 aattgaatat tgtttaagac agcttcttcg tgaggagtgc gaacatcagc acgaatagct
     2101 tcgacaccag tttctgattg catggtatca agtagggtta atcctccaaa aaccttgcca
     2161 acaagatctt tttcttgaag agaaagtttg cgccaatcgt ctaagtcatt tgataaaggg
     2221 atacgggtat caagccaaaa ttgttcggtc aacttttccc aagttgactt gtcaatgacg
     2281 tcttcgattt cattccagtt aattgcttca taatatgtag tcatagaaaa atgatctcct
     2341 aaagagtagt aaagtgcgtt aaggatatcg agataaacca gtagaaccga ccaaagttct
     2401 accggtaata ataagtttaa atagattcaa aagggtaacg aatgagtcag tgagtggttt
     2461 aggcggaacg cctaaagcga ttgtccaaga agtaatagat gctttagcat cattagggca
     2521 tatcgaggaa ttacgacacg gagtaaggaa agtcgataat actttcctta cgtagttagt
     2581 gagtggttta ggcagatcgc ctaaagcgac tgccgtagaa gtaatagatg ctttagcatc
     2641 attacttctt aaccaattaa atgacacaag attcacattg gtttgcgccc acttcttccc
     2701 catcatccgt aaaggtacgg atatagtaga tagatttaat gcctttattg aaagcgtagt
     2761 ttcgtaagat ggataaatca cgagtggttt gtttgctttg tgttttccac tcataaagct
     2821 ccataggcaa ctcactacga aggaatagag ttaatgacaa gccttggtcc acatgttcgg
     2881 tcgcagcggc atagacatca ataactttgc gcatgtccat atcgtaagca gatgtatagt
     2941 aaggaatggt atccgtagac aaaccatttg caggatagta gattttacca attttctttt
     3001 cttgacgttc ttcaattctt tgtgtgattg ggtgaataga agcagagcag tcattgatat
     3061 aagaaataga gccatttgga gcaactgcta gacggttttg atgataaaga ccatcttttt
     3121 gaacggcgtc gcgaagagcc tcccaatctg aagcttgcgg aataaagtga tctttaaaga
     3181 gacttttgac caaatcagat tttggaacaa agtgtcctgt aacgtattta tcaaagtaac
     3241 taccattagc gtacttagag ttctcaaagc caacaaaggt agtttggcgc tcacgagcga
     3301 tgttattgga ttcgaccaag gtccaataat tcaggagcat aaagtaaata tcagtaaact
     3361 cgatggattc tggactgcca tattcaatat gatgttgagc aaggtaagaa tgtagtccca
     3421 tagctccaag gccaaaagta tgagcttggc tattgccatg tttaatggtt ggaacagctt
     3481 caatgcttga tgaatcagta acaaaagtta gggcacgtgt catggtctta atagaacggc
     3541 caaagtctgg tgaggtcatc atgttcagga tattagtgga acctaagtta catgaaatat
     3601 cagttcccat ttctacaaac tcttgcgcat cattgataag gctaggtgtt tgaacttgta
     3661 aaatttcaga acacaagttg ctcatgatga tttttccatc gataggatta gctttattag
     3721 ctgtatcaat attgatgata taagggtaac cagattcttg ttgtaatttt gaaatctctg
     3781 tttcaagatc acgagcttta attttagtct tagtaatttt agggttcgcc actaactcat
     3841 cgtacatatt ggtaatgtcg agatagttaa agggaatgcc atattctttt tcaacattgt
     3901 aaggactaaa gagatacatg tcctcgtttt tacgagctaa ttcgtagaat ttatcaggaa
     3961 cggtaatccc tagtgacaag gttttaacac gcaccttttc atcggcattt tcttttttag
     4021 tagataagaa agcaatgata tcaggatgaa aaacatttag gtaaacaaca ccagctcctt
     4081 gacgttgccc aagttgattt gaataagaaa aactatcttc aaataatttc ataacaggaa
     4141 caactcctga ggctgcacca gcatagcctt tgattggtgc gccagcttca cggaggttag
     4201 acaaggtaat cccaacacct ccaccaatac gggataattg caaggcagag ttaatggaac
     4261 gtccgataga attcatgtca tcagttactt gaatcaagaa acaagagacc aattcaccac
     4321 gacggcttcg accagcattt aaaaaggaag gagtagccgg ttggtaacgt tggttaatca
     4381 tttcaacggc taaatctttt gctaagtctt cttgaccatc tgcaaaatac aaagcattaa
     4441 acaagacacg gtcttcaagg ttttctaaat aatgctctcc atcatttgtt tttaaggcgt
     4501 attgctggta gaacttgtag gctgccataa atgatttaaa gcgaaaattt tctgatttga
     4561 ttatgctatc taattcagta ataaattcag gacggtattt ctgaataaaa gctgattcaa
     4621 tgtaatcatt tgagattaaa tactcaattt tttccgtaat ggaagtaaaa gacatggtgt
     4681 ttggcagcac attttcagcg aaaaaagctt ttaaagcttc tttgtcttta tgaagtggaa
     4741 ttttaccatt aacaggacgg ttaatttcat tatttaggcg aaaatatgaa atatcgccaa
     4801 tatctttgag actcatatta tttttccttg tcttattaaa tcaattcttt taactttgca
     4861 ggttggaagc cagaaaatac gaggttatct gcttcgataa caggagcaga tgtgaagcca
     4921 agacttttca cataatccac tttttcagga tgttcatcga tattaatttc ctggaagttg
     4981 gctccgtgtt gttctaaaaa tttcttagtc attttgcact gcatgcaatt atttttggaa
     5041 tatactgtaa tcatgtcttg tccttctaaa attagtaatg gttccatctt aaatggaatt
     5101 actataatag catataaaaa ataaagctaa aaagcaagct ataaacttat ctaaaacaca
     5161 acatattgtg ttgaaataaa aaacagaaac ctaaatattg tgtcaatctg gattcttatg
     5221 gttagagctc tagcgttttg gtgatagaag ctaagcagtt ttaataaagc attaaagtct
     5281 gaaaaataaa ttgtgaaaaa cgtgaaaata aaagaaaata atttcacatt ttttgtcgtt
     5341 taaatattta atcttgaaaa taaaaaagca ttgtgctata atcggttgtt gtaagggtta
     5401 tcatgtgatg gctcaaaaat acacaactta aaaggagaac catactatgg cttcaaaaga
     5461 ctttcacatt gttgcagaaa caggtattca tgcgcgtcca gcgactttgc ttgttcaaac
     5521 agctagcaaa tttgcttcag acatcactct tgactacaaa ggtaaagcag taaatctaaa
     5581 atcaatcatg ggtgttatga gtcttggtgt tggtcaaggt gcagatgtta ctatctcagc
     5641 tgaaggtgca gatgctgaag atgccattgc agcaattgaa gaaacaatga ctaaagaggg
     5701 attggcataa gactatgaca gaaatgctta aaggaattgc agcctcagac ggcgttgctg
     5761 ttgctaaagc atatctacta gttcagccgg atttgtcatt tgagactgtt acagtcgcag
     5821 atacaaatgc agaagaagct cgccttgatg ttgcactcca agctgcacaa gacgagcttt
     5881 ctgttatccg tgaaaatgca gtagaaagct taggtgaaga agcagcagcc gtttttgatg
     5941 cccatttgat ggttcttgct gatccagaaa tgatcagcca ggttaaagaa acgattcgcg
     6001 caaaacaaac gaatgcagaa acaggtctta aagaagtgac tgacatgttc atcaccatct
     6061 ttgaaggcat ggaagataac ccatacatgc aagaacgtgc agcggacatc cgcgacgttg
     6121 caaaacgtgt gttggctcac cttttaggtg taaaacttcc aaatccagct acaatcaatg
     6181 aagaatcaat cgttatcgca cacgatttga caccttcaga tactgctcaa cttaacaaac
     6241 aatttgtaaa agcatttgtt acaaatatcg gtggtcgtac aagtcactca gctatcatgg
     6301 cacgtacact tgagatcgct gcggtacttg gaacaaatga tattacaaaa cgtgttaaag
     6361 atggtgatgt gattgccgtt aatggtatca ctggtgaagt gattatcgat ccaagcgaag
     6421 atcaagtact tgcttttaaa gaagctggtg cggcttatgc caaacaaaaa gcagagtggt
     6481 ctctccttaa agatgcgcac actgaaacag ctgatggcaa acactttgaa ttggctgcta
     6541 atatcggtac gcctaaagac gttgaaggtg ttaatgacaa tggcgctgaa gctgttggcc
     6601 tttaccgtac tgagttcttg tacatggatt ctcaagactt cccaactgaa gacgaacaat
     6661 acgaagctta caaagcagtg cttgaaggca tgaatggcaa acctgttgtg gttcgtacaa
     6721 tggatattgg tggagataag gaacttcctt actttgacct tccaaaagaa atgaacccat
     6781 tcctcggttt ccgtgctctt cgtatctcca tctctgaaac tggggatgcc atgttccgca
     6841 cacaaatgcg tgcgcttctt cgcgcctctg ttcacggaca acttcgtatc atgttcccaa
     6901 tggtagcact tcttaaagaa ttccgtgctg caaaagcagt ctttgacgaa gaaaaagcaa
     6961 acttgcttgc agaaggcgtt gcggttgctg atgacatcca agttggtatc atgattgaga
     7021 ttcctgcagc tgctatgctt gcagaccaat ttgctaagga agttgatttc ttctcaattg
     7081 gaacaaacga ccttatccaa tacactatgg cagcagaccg tatgaacgaa caagtatcat
     7141 acctttacca accatacaac ccatcaatat tacgtttgat caacaatgtg atcaaagcag
     7201 cgcacgctga aggtaaatgg gcaggtatgt gtggtgagat ggcaggtgac caacaagctg
     7261 ttccacttct tgttggaatg ggcttggatg agttttctat gtcagcaact tcagtacttc
     7321 gtacgcgtag tttaatgaag aaacttgact ctgctaagat ggaagaatat gcaaatcgtg
     7381 cgcttacaga atgttcaaca gcagaagaag ttcttgaact ttctaaagaa tacgtttctg
     7441 aagattaacg ggttaaagag ttggtttaaa ccagctcttt tttgctagct aaaaatacaa
     7501 ttactttttg gtgtgaaatc atttgagtta tcaaataatt atcagaattt tcagatattt
     7561 agtgtagcca aatcttgaat tatatcagaa aagtgataga ataagattac tatcataaaa
     7621 ggagataaaa cgttggcaaa acaatataaa aatttagtga acggtgaatg gaaactatca
     7681 gaaaacgaga ttaccattta cgcaccagca acaggtgaag agttaggatc agttccagcg
     7741 atgacgcagg cagaggtaga tgctgtttac gcttcagcta aaaaggctct atcagattgg
     7801 cgcgccttgt cttatgtgga acgtgcagct taccttcata aagcggctga tattttagta
     7861 cgtgatgctg aaaagatcgg cgcgattctt tcaaaagaag tagccaaagg tcacaaggca
     7921 gctgtcagtg aagttattcg taccgctgaa atcattaatt atgcagcaga agaagggctt
     7981 cgtatggaag gtgaagttct tgaaggtggt agcttcgaag ccgcaagtaa gaagaagatt
     8041 gctattgttc gtcgtgaacc agttggttta gttcttgcca tctcaccttt taattatccc
     8101 gttaacttgg caggttctaa aattgctcca gcccttattt caggaaatgt tgttgctctt
     8161 aaaccaccaa cacaaggctc tatttctggt ttgttactag cagaagcttt tgcagaagct
     8221 ggtattccag caggtgtctt taataccatt acagggcgag gttctgttat tggggattac
     8281 atcgttgagc acgaagcggt taactttatc aactttacag gttctactcc aattggggaa
     8341 ggaatcggta aattagcggg tatgcgacca attatgcttg agcttggcgg taaggattct
     8401 gctatcgttt tggaagatgc agatcttgtt ttagcagcga aaaatattgt agccggtgct
     8461 tttggttact caggacaacg ttgtacagcg gttaaacgcg ttcttgtgat ggacaaggtg
     8521 gcggatcaat tggcggctga gattaaaaca cttgttgaaa aactaagtgt cggaatgcct
     8581 gaagacgatg ctgatattac accattaatt gatacatcag ctgctgattt tgttgaaggg
     8641 ttgattaaag atgcaactga taagggagct actgctttga cagcctttaa tcgtgaaggc
     8701 aatcttattt cacccgttct ctttgatcat gtgacaactg acatgcgttt ggcatgggaa
     8761 gagccgttcg gcccagtatt accaattatt cgtgtaacca ctgtagaaga agccatcaag
     8821 atttctaatg agtctgaata tggtttgcaa gcttctattt ttacaactaa tttcccaaaa
     8881 gcttttggca ttgctgagca attagaagtt ggaactgttc accttaacaa taaaacacaa
     8941 cgtggaacag ataatttccc attcttaggc gctaaaaaat caggtgcagg ggtacaagga
     9001 gttaaatatt ctatcgaagc tatgacaact gttaaatctg ttgtatttga tatccagtaa
     9061 taaaaaaaat aaacaaaaag ttaggata
//
LOCUS       DATUYR010000055         9000 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.055, whole genome shotgun sequence.
ACCESSION   DATUYR010000055 DATUYR010000000
VERSION     DATUYR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.055"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            314..796
                     /locus_tag="VCI33_001357"
     CDS             314..796
                     /locus_tag="VCI33_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEP2832277.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            885..1937
                     /gene="citC"
                     /locus_tag="VCI33_001358"
     CDS             885..1937
                     /gene="citC"
                     /locus_tag="VCI33_001358"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEP2832278.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGGGFLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDQSLVPFSVRKMLIQEGLKHLDNIIFHETGPYLISQ
                     ATFPAYFQTEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDSDWDLLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(2047..3441)
                     /locus_tag="VCI33_001359"
     CDS             complement(2047..3441)
                     /locus_tag="VCI33_001359"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEP2832279.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFV
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKII
                     GDEAVIACRPADLIEPQMIYLRDEIAQYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(3456..4034)
                     /gene="citX"
                     /locus_tag="VCI33_001360"
     CDS             complement(3456..4034)
                     /gene="citX"
                     /locus_tag="VCI33_001360"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEP2832280.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEVFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            complement(4027..5559)
                     /gene="citF"
                     /locus_tag="VCI33_001361"
     CDS             complement(4027..5559)
                     /gene="citF"
                     /locus_tag="VCI33_001361"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEP2832281.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPI
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHP
                     SAVSLGKHADHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINP
                     NRQDLVDHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            complement(5562..6449)
                     /gene="citE"
                     /locus_tag="VCI33_001362"
     CDS             complement(5562..6449)
                     /gene="citE"
                     /locus_tag="VCI33_001362"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEP2832282.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIV
                     DVEAVIERVERENSIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTEKEIDHSKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            complement(6437..6745)
                     /gene="citD"
                     /locus_tag="VCI33_001363"
     CDS             complement(6437..6745)
                     /gene="citD"
                     /locus_tag="VCI33_001363"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEP2832283.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEKTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGVDQYDWKEIDSWNV
                     "
     gene            complement(6933..8054)
                     /locus_tag="VCI33_001364"
     CDS             complement(6933..8054)
                     /locus_tag="VCI33_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEP2832284.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            complement(8099..8494)
                     /locus_tag="VCI33_001365"
     CDS             complement(8099..8494)
                     /locus_tag="VCI33_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEP2832285.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            complement(8551..8874)
                     /locus_tag="VCI33_001366"
     CDS             complement(8551..8874)
                     /locus_tag="VCI33_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832286.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSAIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
BASE COUNT         2647 a         1857 c         1793 g         2703 t
ORIGIN      
        1 acagcttctt taacatcaat tggttgaaaa caagaatcag ataacattat cgttagatag
       61 actgaagaat caaaaacaca gtttgttaca taaataaatt cttgtcaaaa tttgtatttt
      121 ctatttgaac tgatacgatt gacaaaggct aaactaaaat agcttgactt ttttagaatc
      181 tttgtgtttt ttgtcggctc ccctcctatt ttttccttga attgatctat ttaacctttt
      241 agaacaaggt ttatcgcata ctatttttag acattttgtt ataataaaaa gaaacgactt
      301 gaggagataa aagatgccaa agagaataag aaaagctcta ggagtagttg ggaaactgat
      361 gtcaattgtt cctgacacta ctgaaattat tggaaaaacg attgataaca gtcgaccaat
      421 tatcgaaaaa cgtatggaac aaaaacatga aaaagagatg caactacgca cgatagatga
      481 tgtgattaat cttcctgttg atcaggcaca agctcatttg gaacaacttg gctttgtagt
      541 agccactata cctgctaagc ctcataaaaa atggcttcat agtaatctca atgaagtggt
      601 tgctatgtct ccaaaatctg gtaaatataa gataggaagc ctgatcaaac tctactacat
      661 tactgttgat gttttggaaa aaagtcaaga cttattagac caagaaaact tgcggactgt
      721 ggaacgcaat caaaaaatcg ctgatacttt tgagtctgtc aaacacattc gatttccttt
      781 taaaaaatgg cgttgataaa aaacagagtt gcctttagat agatcagcct gttttggtac
      841 taagttatgt tatgattcaa taaaattaaa gatacggaga agaaatgcct tactacacta
      901 tttcaaaagt ctttccttca gataaaacga caatggctag tgtaaagaac ctgcttcatc
      961 aagaaggtat tcgtttggat gctcatttag attatacttg tgccatcatg aatgcgcaaa
     1021 atgatgtgat tgctaccggt tcttactttg gaaatagctt gcgctgtctt tgcgtatcca
     1081 gtgcctatca aggtggagga tttcttaata ggattgtgag tcatctcatt gatgaagaat
     1141 atgcccttgg caactatcat ctttttgttt atacgaagac ctcttctgct gcttttttca
     1201 aagatcttgg ttttactgaa attgtccata tcgacaatca catcagtttc ttggaaaata
     1261 aaaaaactgg ttttcaagat tatctgatga cacttaataa gcctgagcaa acacctggta
     1321 aagtcgctgc tattgttatc aacgctaacc cctttacctt agggcatcaa tttttagtag
     1381 agaaagctgc aagagaaaat gattgggttc atctgtttat ggtcagtgag gaccaaagct
     1441 tggttccttt ttcagtacga aaaatgttga ttcaagaagg attgaaacat cttgataaca
     1501 ttatctttca tgaaacaggt ccttatttga ttagtcaggc aacctttcca gcttatttcc
     1561 aaacggaaga caatgatgtg atcaaaagcc aagctttgct ggatactgct atttttctaa
     1621 agattgctca aaccttacag attacaaaaa gatacgttgg agaagagcca accagccgag
     1681 taactgctat ttacaatgaa atcatggcag agcagttgca gcaagctggt atcctcctag
     1741 atattttgcc aaggaaagct atcaatcagc agcaagatcc tatcagtgcc tcaacggcta
     1801 ggcaggcatt gaaagatagt gattgggacc ttctggcaaa actccttccc aaaacgtctt
     1861 tagattattt ttgttcgcta gaagcccaac ccattattaa aaagatacaa gccacttcat
     1921 ctgtcaaaca ttactaagat aaaaaaccta tctgattatc aaaatcaggt aggtttttgg
     1981 tattgttctt acagcaaatg cgtttcacca aggagattaa tggttaagag gattagtcat
     2041 tccctgttag tcttgaatgt ccagttgtac agtaacttct tgaaccggaa catcatagaa
     2101 aggatcttca cggcgtccca agaaatctct ggcttgctgt gggaaactcg catagcttaa
     2161 cacgtcttct tctgaacgag cgtattgagc aatctcgtca cgtagataaa tcatttgagg
     2221 ctcgattaag tcagctggtc tgcaagcaat gactgcttcg tcaccaatga ttttctcttt
     2281 gataccttct gctaatgggg ctggtgattg accgtatagc cctcgaacat agtctttgat
     2341 ttcatttgga actaccttgt agcgttcccc tgagatgata ttcatcaagg cctgtgttcc
     2401 aaccatttgt gataatggcg ttacgagtgg cggataccca agatcagccc ttactttagg
     2461 tacttctgct aagacttctt cgtatttatc agctaggcct tgctcggtta attggctcaa
     2521 taggtttgat agcatgccac ctggaacttg gtaaatcaat gtttttggct cagtatcttt
     2581 gactttagga tttaaaatcc ctatttctcg atagtggtca cgaatagtgt tgaaatattc
     2641 tgcaactttg gccacctctt gcatatccaa gcctgtgtca aagcctaaat ccgtcaaggc
     2701 aatcgccatt gactctgtgg caggctgact ggttccccct gaaaaagagg aaatcgccgt
     2761 atcaataata tcagctcctg cttctgctac ttttaagtaa gtcatttctg aaataccact
     2821 ggtagcatgg gtatgaacct caagaggaat agttgtattc tccttgatac atttgaccaa
     2881 ttggtaacct atttcaggag ttaagacacc agccatgtct ttgatacaaa tggagtccgc
     2941 tccgatagct tggtaagctt tcgccaattc aacaaagtag tcaatggtat gtaccggact
     3001 tgtcgtgtaa gaaatggcaa cttgagcatg ccctccaaat tttttagtcg ctgaaacagc
     3061 tgtttgcaag ttgcgagggt catttaaagc atcgaagata cggacaatat caatcccgtt
     3121 ttcaatggat ttttgaacga aggatctgac cacatcgtca gcataattgc gatatccaag
     3181 aaggttttgt ccgcgtaaga gcatctgaag tttagtcttt ttcactgctt tacggatggc
     3241 tcttagccgt tcccaagggt cttcatttaa aaagcgtagg caggaatcaa aggttgctcc
     3301 tccccacatt tcaagggcat gataaccagc attgtctagc gtttcgagaa taggaatcat
     3361 ttccttggtt gtcatacgtg tggcaatctg actttgttga ccatcacgta aaactgtttc
     3421 cgttatacgg attttcgtca taattgttct cctcatcaac ctgtcttatc tgatgacgat
     3481 gattggttgt ctttattgaa aaatgaatgg agtaattttg aaatagcagt ctgcatctcc
     3541 tcgacactgt gcttacgtag gcgaccgcag actttggcct ctttagaaca gataaaacat
     3601 tgcctagggg agcctcctaa tacggttctg ctaattgaat gaggcagatc attttgcaag
     3661 accaagacat ctaaatccat gagacgacct attggcaact ctgtctctaa ggctatcatt
     3721 tttaacttca ggtcgcggct aggtagactt gtgatgaggt aatactcata acccgttgta
     3781 ggcagtaatc gcaactggta acaaatctta tcgtcagcta aggcagtttg aatggcctta
     3841 atcactatat caaaaacttc aagaagttta ggagaggttt taattggtcc agggatattc
     3901 atggtgaccg ataataagct accttctggg tactccttta ataaatgcag ctgacgcaga
     3961 gctctttctt cgcgggctct taacatatca ctaagttgga tagcttcgcc tgaaaaataa
     4021 gtgtctttac acattgtaaa ccacatccat gaggctacca tcacggtatt caataagagc
     4081 aacaacctta tcaccatatt gaatacgctc aggtgtgcca acgatagcgt aagccttctc
     4141 ttttagctcc tcaatactaa attgtggcac attgagggat ttgaaatggt ctactaagtc
     4201 ttgacgattt gggttaatgg caattcccac ttctgtgacg accacatcga cacttgttcc
     4261 tggtgtaatg accgtattaa cctcatctac aaaagttggg atgcgtccgc ggataagtgg
     4321 tgaaataacc aaactcatct tagccgcaaa ggcagtgtcg cagtgtcctc cagaagcgcc
     4381 acgaatcacg ccgtcagatc ctgtcatcac attaacattg aagttagtat cgacttcaag
     4441 ggctgataaa atacaagtgt ctaattgatt gataacagca cccttgctca aaggtgaggc
     4501 atacatgttg gcatcaattt cataatggtc agcatgctta ccaagagaaa cagctgatgg
     4561 gtgatcaaag tcttggacat caagaatttt ttcaaccagt ccttcttcga gcaactccac
     4621 catagcattg gtaataccac caagagcaaa gctagctttg atattttctt taatcatagc
     4681 ttcacgcatg aaacgggtta ctgccaaaga agcgccacct gttccagttt ggaaagagaa
     4741 tccttcttta aaataaggag agttggtgat tactttagcg gcgtattctg caattaagag
     4801 ttctttagga tttttcgtaa aacgagttgc ccctttggcg attccttgag gatctccgat
     4861 agcatccact gttaccacat agtcaacatc tgtttgagga atgctgattg gggtattagg
     4921 ataaggaacc aaattatctg tcaagataac cacctgatcc gcatatttgg catcaatcat
     4981 ggcatagccc aaagaaccac aggttgcttt cccttttgtc ccattaacat ttccgtaagc
     5041 atctgaactt ggggcgccca gaaaggcaac atcaatatgg atatccccac tagcaatggc
     5101 acgagcgcga ccaccatggg agcgaataac cacaggattt tccattagtc cttctgagat
     5161 ggctgcccct actttgtcac gaagaccaga tgaagtgatg ttagtgacca caccattttt
     5221 gatgtgatca atcaaaggct catgtacatt agcgatggaa cttggggcaa tagacaggtt
     5281 tttaatgccc attttggcaa tttcctccaa gaccatattc ataatgaaat ccccttcacg
     5341 gaaatggtgg tggaaagaaa tggtcatgcc atctgttaaa cctgtttttt caatcgcttc
     5401 acgaacagat cctagtaatt tagaatctcc aggcttaact ggtttaatac ggcgacttga
     5461 ttcgtcatac tgtttgatat ttgcgagttc tccttcaaag acaccgtatt ggtcggcgta
     5521 tggttgagga atatctctac cgagtttatt tgttaccatc tttaaatgtc ctcctcagat
     5581 aaaacacctg ctgctgttgc tagggcaatg acacgttctg cacgttcaac gattggttta
     5641 tcaaccattt ttccatttaa ggaaataacg ccagagcctt tgctttcagc ttcacgaatt
     5701 gcccagatga cttgtttgga atggtcaatt tctttttcgg ttggggtata aatctcattg
     5761 accagaggaa tttggcgagg gttgatgacc gatttaccgt caaatcctaa ctgtttgatc
     5821 atacgaactt cgttttggaa tccttctgta ttattgacat cagaataaac agtatcaatg
     5881 gcagcaattc cagcagcacg agcagcatgt aaaatcatgc tacgagcaaa gaataattct
     5941 tgaccatctg ggtaacgacg cgttttcata ttcgtgacat agtcttctgc cccaagagca
     6001 ataccaatca agcgcttaga agctttggca atctcacgag catttagaac accttcggct
     6061 gattcaatgg ctgccatcat acgtgtgcga ccaacttcaa tgctgttctc acgttcgaca
     6121 cgttcaataa cagcctccac atcaacgata tcttgtgcag tctctgtttt tggaagacga
     6181 atcacgttga cacctgccaa aacaacagct tcgatgtcta aagccccaca agaatcaaga
     6241 ccattcacac gaacaaccgt ttcaacgctc gaataatcaa aagtttttag cgcaaaatgg
     6301 acgagagctc tagaagtgtc tttttctttg agtgaaacag aatcttccaa gtcaaacatg
     6361 attgaatcag caccaaacaa aggagcatct cgaagcatag cagcattggc acctggcaca
     6421 aacatcattg ttcttcttaa acgttccatg agtcaatctc cttccaatcg tattggtcaa
     6481 caccagctgc gcgatgaaca gctgcgatgg ttctcgcttg aatggtgcaa tctaaagcgc
     6541 ctttatcaac agcctctact ttagcagccg tcaccttcaa attcactagg gtttgatgaa
     6601 tgagttgacg aatgtgattg ccaaattgtt tttcaacact actgtctagg gttattgtaa
     6661 tggttttctc gtcattaggg gatactgtaa tcattaggtc acttgattca agtgaaccag
     6721 caacggcagt ttgtttaata tccattgcgt ttacctcctt gattttctta aatgatattg
     6781 ttatttacta actgggaaaa gtttgatcag gatctctgcg acaagataga gaattcctaa
     6841 cacgataaag acacctgcca taccaagcgc cattagctca aaggaactaa tcaaatggtc
     6901 catattaatt gtcataagct ttccttcctt tcttagctaa agtaagctag caacaaacca
     6961 cctgcgataa cagaggcgat ttggccagag acattggcac ctacagcata catgaggata
     7021 aagttttgcg ggtcttcatc tgttgccatt ttttgaatga cacgacttga cattggaaaa
     7081 gctgaaatac ccgcagcgcc aatcattgga ttaatcttct cttttcggaa gagattgaga
     7141 aatttggcaa acatgacccc accgatagaa tccatgataa aagcaagtaa accaaagacg
     7201 ataattaaga gtgtctgtac atttaagaac aggtctgcct gcattttaat agagatggtc
     7261 aatccaagca agatactaat aatgtttacc aattcatttt gagcagtttg tgacaggcga
     7321 tcgagcacac cgcactcacg taataagtta ccgaacatca aaaagccaac taaaggtaga
     7381 gaaattgggg cgatgaaccc tgctactaag gtaatgatga ttgggaataa gattttagtc
     7441 atttgagata cattctcagc tttgtaagtc atacggatac ggcgctcttt tttcgttgtc
     7501 actaatttaa tggcaaatgg ttggataata ggaaccagag ccatgtaaga ataggctgca
     7561 accgtaattg gacccagtaa atccttggcc agttggttgg caacaaagat agaagtcggt
     7621 ccatctgctg ctccgataat gccgattgaa gctgcttctt taatgtcaaa gccagccagt
     7681 acagcaacaa caacaacaaa gaaaatccca aattgcgctg cagcaccaaa caagagcatg
     7741 aatgggtttt gaagcaaggg accaaagtca atcatggcgc caataccaat gaaaatcaac
     7801 agtgggaaaa gttctgttcc aatcccaaaa ttgaagagag cctcaaaaac cccttcttgc
     7861 tcgactccgt taaccacttg ggttaaaact cctgaaccag ggaagttaac gagaatggtt
     7921 ccaagtccca tggggacaag taaggttggt tcgtattctt ttttaattcc tagatacatt
     7981 aagagggcac cgatgaccat cataacaatt tgaggaatgg tgatggaggt tattcctgcg
     8041 attaaagtgt ccacaaattc acttcctttt ctgtctattt attctagtta tagtactatt
     8101 aaccaattgt aatgagacca tcaccaggat tgaccacttg acctggaccg acgtggatag
     8161 ctgtaatggt tcctgctgat gaggccacaa tctcattttc catcttcatg gcttctaaaa
     8221 tcaatagtgg ctgattttca gttacttggt ctcccactgc tactaagact tttaagatgg
     8281 ttccaggcat tggtgatgga atggcatccg ctcccgctgc agctactggc tgtggtgctt
     8341 gcgcttcctc cacttgtggg cttgcttcag tagggacagg tactggagtg gatattggtt
     8401 gagcgggggc agctgcttgg gcaggagcac cgatttcttc catttctaca agatattctt
     8461 taccgtcaat ggtaatttta aattttcgta acatcatttt tctcctatat gttattgtgt
     8521 catgagttcg tctaaagtat cgttcaacga ctaccttttg caatagatgg atgtcacccg
     8581 aaactgactt tctgggtaaa cacctgcagc gatacttgaa gcaattaaag aaacagtgat
     8641 tgcctcagga ttgcgcttga gaatgcgttt aaccttgaat tgactattag gtctatcgcc
     8701 agcagcaata gcactggtaa ttaaagtaac ctttttgtaa tcacttttgt ctgcaggaat
     8761 ataagcggga atcttttccc agttttcaga aggttctatt tctgtactaa ggggcgagga
     8821 ttctttcaac ttctcttttt tagagtgttt aaaccatttt tgtaaaaact gcatgagcta
     8881 cctctttttc acatttacct atttagggaa ctagtaagtt tattatatac taccaatctt
     8941 ttttgagatg gcttttttgt gttctttcta agagcgcttt caatgtttca taaagttttt
//
LOCUS       DATUYR010000056         8981 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.056, whole genome shotgun sequence.
ACCESSION   DATUYR010000056 DATUYR010000000
VERSION     DATUYR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.056"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            88..1008
                     /gene="lmb"
                     /locus_tag="VCI33_001367"
     CDS             88..1008
                     /gene="lmb"
                     /locus_tag="VCI33_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEP2832287.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1021..3498
                     /locus_tag="VCI33_001368"
     CDS             1021..3498
                     /locus_tag="VCI33_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEP2832288.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGSATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPAPGRRKAPIPDVTP
                     NPGQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEED
                     GLIFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDH
                     SKPSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHF
                     HYIGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGK
                     VGYMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSS
                     LPMHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPG
                     HEESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKE
                     TFVWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQ
                     ADKSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIF
                     QPEGVQFYNKNGELVTYDIKTLQQINP"
     gene            3667..3867
                     /locus_tag="VCI33_001369"
     CDS             3667..3867
                     /locus_tag="VCI33_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP2832289.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(3949..4575)
                     /locus_tag="VCI33_001370"
     CDS             complement(3949..4575)
                     /locus_tag="VCI33_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832290.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHEFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(4559..5362)
                     /locus_tag="VCI33_001371"
     CDS             complement(4559..5362)
                     /locus_tag="VCI33_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832291.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGNLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(5374..6195)
                     /locus_tag="VCI33_001372"
     CDS             complement(5374..6195)
                     /locus_tag="VCI33_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832292.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNASLRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVSLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVVFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6192..7169)
                     /locus_tag="VCI33_001373"
     CDS             complement(6192..7169)
                     /locus_tag="VCI33_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832293.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRVKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7282..8910)
                     /locus_tag="VCI33_001374"
     CDS             complement(7282..8910)
                     /locus_tag="VCI33_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP2832294.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQINIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELADKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINIGKQGVHSHGHDWSLLTNIAEWTWDESTK"
BASE COUNT         2945 a         1760 c         1785 g         2491 t
ORIGIN      
        1 aagatggtag ttgccattga caaagcattg ataaaggagt aaaattaact ggttaataac
       61 tggttaaatt ataattgagg aggtactatg aaaaaaggtt tttttctcat ggctatggtc
      121 gtgagtttag taatgatagc agggtgtgat aagtcagcaa accccaaaca gcctacgcaa
      181 ggcatgtcag ttgtaaccag cttttaccca atgtatgcga tgacaaaaga agtatctgga
      241 gacctcaatg atgtgaggat gatccaatca ggtgcaggca ttcattcctt tgaaccgtct
      301 gtaaatgatg tggcagctat ttatgacgcg gatttgtttg tttaccattc acatacctta
      361 gaagcttggg caagggatct agaccctaat ttaaaaaaat caaaggttga tgtgtttgaa
      421 gcgtcaaaac ctttgacact agatagagtc aaagggctag aagatatgga agtcacacaa
      481 ggcattgacc ctgcgacact ttatgaccca catacctgga cggatcccgt tttagctggt
      541 gaggaagctg ttaatatcgc taaagagcta ggacgtttgg atcctaaaca caaagacagt
      601 tacactaaaa aggctaaggc tttcaaaaaa gaagcagagc aactaactga agaatacact
      661 caaaaattta aaaaggtgcg ctcaaaaaca ttcgtgacgc agcacacggc attttcttat
      721 ctggctaaac gattcggctt gaaacaactt ggtatctcgg gtatttctcc agagcaagag
      781 ccctctcctc gccaattgaa agaaattcaa gactttgtca aagaatacaa cgtcaagact
      841 atttttgcag aagacaatgt caatcccaaa attgctcatg ctattgcgaa atcaacagga
      901 gctaaagtaa agacattaag tccacttgaa gctgctccaa gcggaaacaa gacatatcta
      961 gaaaatctta gagcaaattt ggaagtgctc tatcaacagt tgaagtaaag gagattatta
     1021 gtgaagaaaa catatggtta tatcggctca gttgctgcca ttttactagc tactcatatt
     1081 ggaagttacc aacttggtaa gcatcatatg ggttcagcaa caaaggacaa tcaaattgcc
     1141 tatattgatg atagcaaagg taaggcaaaa gcccctaaaa caaacaaaac gatggatcaa
     1201 atcagtgctg aagaaggcat ctctgctgaa cagatcgtgg tcaaaattac tgaccaaggc
     1261 tatgtgacct cacacggtga ccattatcat ttttacaatg ggaaagttcc ttatgatgcg
     1321 attattagtg aagagttgtt gatgacggat cctaattacc attttaaaca atcagacgtt
     1381 atcaatgaaa tcttagacgg ttacgttatt aaagtcaatg gcaactatta tgtttacctc
     1441 aagccaggta gtaagcgcaa aaacattcga accaaacaac aaattgctga gcaagtagcc
     1501 aaaggaacta aagaagctaa agaaaaaggt ttagctcaag tggcccatct cagtaaagaa
     1561 gaagttgcgg cagtcaatga agcaaaaaga caaggacgct atactacaga cgatggctat
     1621 atttttagtc cgacagatat cattgatgat ttaggagatg cttatttagt acctcatggt
     1681 aatcactatc attatattcc taaaaaggat ttgtctccaa gtgagctagc tgctgcacaa
     1741 gcctactgga gtcaaaaaca aggtcgaggt gctagaccgt ctgattaccg cccgacacca
     1801 gccccagccc caggtcgtag gaaagcccca attcctgatg tgacgcctaa ccctggacaa
     1861 ggtcatcagc cagataacgg tggctatcat ccagcgcctc ctaggccaaa tgatgcgtca
     1921 caaaacaaac accaaagaga tgagtttaaa ggaaaaacct ttaaggaact tttagatcaa
     1981 ctacaccgtc ttgatttgaa ataccgtcat gtggaagaag atgggttgat ttttgaaccg
     2041 actcaagtga tcaaatcaaa cgcttttggg tatgtggtgc ctcatggaga tcattatcat
     2101 attatcccaa gaagtcagtt atcacctctt gaaatggaat tagcagatcg gtacttagcc
     2161 ggccaaactg aggacaatga ctcaggttca gatcactcaa aaccatcaga taaagaagtg
     2221 acacatacct ttcttggtca tcgcatcaaa gcttacggaa aaggcttaga tggtaaacca
     2281 tatgatacga gtgatgctta tgtttttagt aaagaatcca ttcattcagt ggataaatca
     2341 ggagttacag ctaaacacgg agatcatttc cactatatag gatttggaga acttgaacaa
     2401 tatgagttgg atgaggtcgc taactgggtg aaagcaaaag gtcaagctga tgagcttgct
     2461 gctgctttgg atcaggaaca aggcaaagaa aaaccactct ttgacactaa aaaagtgagt
     2521 cgcaaagtaa caaaagatgg taaagtgggc tatatgatgc caaaagatgg caaggactat
     2581 ttctatgctc gtgatcaact tgatttgact cagattgcct ttgccgaaca agaactaatg
     2641 cttaaagata agaagcatta ccgttatgac attgttgaca caggtattga gccacgactt
     2701 gctgtagatg tgtcaagtct gccgatgcat gctggtaatg ctacttacga tactggaagt
     2761 tcgtttgtta tccctcatat tgatcatatc catgtcgttc cgtattcatg gttgacgcgc
     2821 gatcagattg caacaatcaa gtatgtgatg caacaccccg aagttcgtcc ggatgtatgg
     2881 tctaagccag ggcatgaaga gtcaggttcg gtcattccaa atgttacgcc tcttgataaa
     2941 cgtgctggta tgccaaactg gcaaattatc cattctgctg aagaagttca aaaagcccta
     3001 gcagaaggtc gttttgcaac accagacggc tatattttcg atccacgaga tgttttggcc
     3061 aaagaaactt ttgtatggaa agatggctcc tttagcatcc caagagcaga tggcagttca
     3121 ttgagaacca ttaataaatc tgatctatcc caagctgagt ggcaacaagc tcaagagtta
     3181 ttggcaaaga aaaatgctgg tgatgctact gatacggata aacccaaaga aaagcaacag
     3241 gcagataaga gcaatgaaaa ccaacagcca agtgaagcca gtaaagaaga agaaaaagaa
     3301 tcagatgact ttatagacag tttaccagac tatggtctag atagagcaac cctagaagat
     3361 catatcaatc aattagcaca aaaagctaat attgatccta agtatctcat tttccaacca
     3421 gaaggtgtcc aattttataa taaaaatggt gaattggtaa cttatgatat caagacactt
     3481 caacaaataa acccttaacc aaaagaagat ctcattgtta aagcactgct ttgtcaaagc
     3541 aagttaccgt gattttgaag tcattttatg taacgagtag tgataaaagt tggataatag
     3601 cggttttctt ttgcaaagaa atggtatcca tgttagaata ataaaaaaaa gaggaggatt
     3661 ctaatcatgt cagaagaaaa attaaaatca aaaattgagc aagcatctgg tggcctaaaa
     3721 gaaggtgctg gtaagttgac aggtgataaa gagctagaag caaaaggatt tgttgaaaaa
     3781 acaattgcta aaggcaaaga acttgcagac gatgcaaaag aagcggttga aggtgctgta
     3841 gatgccgtta aggaaaaatt aaaataatta aaaaaaccag ctgtaagctg gtttttttaa
     3901 tggtgtgatt ttgttaggaa aagactcaat aggtaataac cgtaggtgtt aggtatgctt
     3961 gaacagttga gtacgattct cttcaatcat atagcacgcg tcacaaagtt tttctaacat
     4021 atcaaactca tgactaataa tgatgagacc taaatttcta tcctttacaa tctccaacag
     4081 gcttttccat acactagctt gtgtaatgct atccaacata gtagtcattt catctgcaat
     4141 aaggtatttc gtctctggat gtaatgaacg cacaatcgaa aagcgttgca attcccctcc
     4201 cgagagttca ctagggcgac gctttagcca tttttcttga atgccaaaag catctcgcaa
     4261 atcttgactt ggatagtagg cttcttccaa actttttttc ataggccata aaggattcat
     4321 ggtttgttca ggatgttgtt ggataagttg tacaggtcta aatgctttgt taggcaaatg
     4381 actgccatca actaatactt caccagattt aggctgcaaa aaaccggcaa gcactctgga
     4441 taaacttgtt tttccacacc cactttgtcc aaatatccct aagatttgac caggtgctac
     4501 ctctaaatca atctccttaa aaagccattg atctttttta tgataaaagc caagcttttt
     4561 agcctctaag gtcatgagta actcctttca aaaagtcttg ctgagggagt gtgcgccata
     4621 aacgtctcgc aaattctgtt tgtaattgct ccccacctcc gctaaaaaag ctagctgggg
     4681 ctgtttcaat agcttttccc tctttaaaaa tagttatacg atccgcaatt tgactagctg
     4741 ctacaatatc atgagtgata aatatgacgc ttattccttt atctgcaaag gagcgtagtt
     4801 gatctaaaac catttgcaga gcatctggat gtaatccagg ggtgggctca tccgcaataa
     4861 tcaaagaaac cgtatcacta atacacgttg taaacaaaac acgtcggagc attccgccag
     4921 aaagttggaa aggatacaag ttaccatcac tttcttttaa accaaactgt tgaaacaatc
     4981 cttcttgagt agccttagca ttttctgaga tacccaagcg cacctgatgc ttgactttca
     5041 tagatggatc taaataatta acggattgtg gaatcaacgt catttctttt cctcgcaact
     5101 gtttgatgcg tttagaagtt agtgattgac cacgataaat catatctcct gttacagctg
     5161 catttttagg aagaatatcc ataatagcat gtgctaataa actcttgcct gaaccactag
     5221 cacctataat agctaataac tcaccttttt taacttctaa attcagcgct tggatcggtg
     5281 tggattgaaa tggttttaaa aatcttccgt attgagtgaa ggtgatggag aggtctttaa
     5341 tgcttaataa tgtttctgtc atatctctac ctcctaaaaa tgatccgttt gagggtaaaa
     5401 gagtttcttt aaagattctc cgatagtatc aaaggcattg acaactaaaa taagataaag
     5461 gcctggaaaa accaccaacc accaattgcc aagagagata tgcttagctg cttctgacag
     5521 aatgatacca accgaaggtt gttcggcaga aaggccaaat cctaagaaag tcatggatgc
     5581 ttcatgcaag atgacgtgcg gaaataagag gataaaccca atgaaaattt gagaagcaat
     5641 caaaggcagg atatgatgcc tcacaatata ataaggcgtt tttcccatgc ttttagagag
     5701 ctggacaaag gctttattct ttagatcata gacttcattg cggataagcc ttgctagaga
     5761 gggccaatgg gtaacagccg ttgcaatgat aaccccttga gcccctttac caacaacaaa
     5821 agaaatgaga atcataaaaa tcaaatgagg cataccaata aacaaatcaa ccaaccaggc
     5881 tattatttta tcaataaggc tatttcctag acctgcaagc actccaaaaa cggtcgcaag
     5941 agagactccc ataagggcac ctaataagcc gacttgtaaa gagaaataaa gccctttaat
     6001 cgttctgacg aacatatccc tacctaaacc atctgtccca aaaagatggt ttaaggaagg
     6061 agcgaggttg cgtaaagatg cattggtttc caaaggcgtc ctatagaaat aaaggtttaa
     6121 ggctagaata ctaagaatga gagaaatggc gatacctagt tgccataaaa ccatcgttcg
     6181 acgtttcaat atcatacttt tctccttaac tgtggattga tgatgctatt taaaatatcc
     6241 gcaataagat tgcccgcaaa aacaaataat gtccctatca tcacgatagc tagaaggagc
     6301 ggtgtatcac ttttaagtcc tgcttcagtg agggtagacc ctaagccagg atatgagaaa
     6361 acttgctcag caagaacaga tcctccaaac aattctccaa aataggaaaa atgcagtgta
     6421 atagctggta cgatagcatt tctaaggcaa tgatgtttaa aaatttgcca ctgtgtttcc
     6481 ccacgcgctc tggcaaataa gacatattca ctagaaagca ccgacatcat tttagttctc
     6541 gtatgaaggg tgacattggc aatgcctaga atacttaacg tgaaaacagg taacataagg
     6601 tgcttaaccc gatcagctaa cgtaatatct tgactcaaag tgcctatcgg ggaagaaata
     6661 ccaatcggga accaccccag ctggacagaa aagattaata aaaaaatgag gccaatccaa
     6721 aacgttggta ctgatatctg aaggtaagaa aaccacctga caactcgatc aagtaatttc
     6781 ccttgatgga aagcggataa cgttcctaag ataaatccaa taagacccga taagatccaa
     6841 gagagtccca taagtatgaa agaagcacct gcccgtgatc taataatatc actaacaggt
     6901 tgccgataaa cgagcgaggt ccctaaatgt ccctgtatca catttttcaa ccaaataaaa
     6961 tactggacta gagctggctt atccaagcca tagtggtgag caatcgcttt gtactgagca
     7021 ggggttagtg atgtgtcata attgacactt gccatgactg gatctactgg agattgtttt
     7081 aacaaaacga aggtcaaaac agatacccca aaaataagcg tgacacatct gatgattttc
     7141 cagatgataa taatggtagt acgtttcaca atgaaatctc cttaatcgat aagaaaagga
     7201 gccgaggaaa cgatcagcac agttttgtgt atcttgtttt ctcagactcc aaacttaaag
     7261 tcgctgtatt aactaaacgc gttacttagt tgattcatcc caagtccact cggcaatgtt
     7321 agtcaataat gaccaatcat gaccatgact gtggacgccc tgtttaccta tattgatacg
     7381 tttatcacca atataagtat ggttaaggct caccaaccat acatttggca aatctccaag
     7441 agtagaagca cctgttttgc catcccactg cgctaacttc caatattcgt tagctttgtc
     7501 aaggtcagaa gatgtcattg ctttgtcaag gtacttagtc acggtaggat tgttataaaa
     7561 cgtaatattg gtccaacctt tacctgctag gcttggatga tgtgattcat aaaattgctg
     7621 cgcgtgatga cgtcctccgg cataaagtaa ggctgagtca tgtgacttcg ttgccatttc
     7681 atcccagtta ctagctttga gtttaatagt aatccctagg gctttggctt gctctgctac
     7741 ttcaacggct aagttcgctc gcaattgatc attagtaggg tagtacagat caaacgctgc
     7801 atcaaggtca ccttttttac ggctaccgtc tgcttgttct ttccatccag cttttgtcaa
     7861 caattgctta gctttagcta ctttattatc tttaatggct gtttttggat tccaaaatgg
     7921 tgttttatca ataattgaat aagctggttt accataacca tttaaaaccg tatcgagaac
     7981 tttttgccta tttaaaccaa tagtcaaggc ttttcggatt gctggatcac tagtgacatc
     8041 atttcctaca ggataaccat caggagaatc agtgatgacg ccttttttca cataaggtaa
     8101 tgataagccg cgcacatcat ttgatggaat atcaaggagg cgggtgcctt tgactttttt
     8161 atcagcaagt tctggcgttg cgtagatcat atcaacatca ccagattcta aagctgctag
     8221 tgctgtgttt tcatcaagta agacccaagt ccatttttta aagtatggtt ttttcccatg
     8281 ccaataaggg ttacgaacaa aaatagcttg ttctccagcc ttatattctt ttaccatgta
     8341 aggtcctgaa ccgataggat tgctcttata cttatcattg taatgttttt tagggacgat
     8401 tgggatttca gtcaactgtg ctgtaaatgt cgaatgcgcc tcagtcaaat ggatattgat
     8461 ctgatttttc ccaactactt caacgttctt gatgaaggtt agatcccaag cctttccatc
     8521 tgctttcaac atatcataag taaacttaac atcatcagca gtaacaggct caccatttga
     8581 gaatttaaaa tcatcatgca agtcaaacga ccaagtcagc ccatcttcag agagatggta
     8641 tgttttagca agctctcctt ttatatctag ttcaggagaa cgtttcaata gagtgctatg
     8701 agtgatattc ccttcattgt ggactccata acggtccttt ggatcgaatt catgagggag
     8761 ctttgccccc atagaaacga caagttcgtc ttttggacgt tgtttgcgct gacgttcttt
     8821 tgtttgaggc ttttgttgtt gacatgcaac taaaataagc ccagtcaaaa ataacgtgat
     8881 aatagagaag tattttaggt attttgacac aatcaaatct ccttatttct aattaataaa
     8941 taataatagt aacgactcca tctcattata gcaataaaca a
//
LOCUS       DATUYR010000057         8868 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.057, whole genome shotgun sequence.
ACCESSION   DATUYR010000057 DATUYR010000000
VERSION     DATUYR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.057"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            41..1237
                     /locus_tag="VCI33_001375"
     CDS             41..1237
                     /locus_tag="VCI33_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEP2832295.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     ALRLKK"
     gene            1303..2010
                     /locus_tag="VCI33_001376"
     CDS             1303..2010
                     /locus_tag="VCI33_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEP2832296.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQITGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLKVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADILLPD"
     gene            2073..3128
                     /gene="prsA"
                     /locus_tag="VCI33_001377"
     CDS             2073..3128
                     /gene="prsA"
                     /locus_tag="VCI33_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP2832297.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            3515..6133
                     /gene="alaS"
                     /locus_tag="VCI33_001378"
     CDS             3515..6133
                     /gene="alaS"
                     /locus_tag="VCI33_001378"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEP2832298.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            6494..6709
                     /locus_tag="VCI33_001379"
     CDS             6494..6709
                     /locus_tag="VCI33_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2832299.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            6706..7467
                     /locus_tag="VCI33_001380"
     CDS             6706..7467
                     /locus_tag="VCI33_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEP2832300.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            7486..8181
                     /locus_tag="VCI33_001381"
     CDS             7486..8181
                     /locus_tag="VCI33_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP2832301.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIAGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(8275..8802)
                     /locus_tag="VCI33_001382"
                     /pseudo
     CDS             complement(8275..8802)
                     /locus_tag="VCI33_001382"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2767 a         1524 c         1835 g         2742 t
ORIGIN      
        1 ttaaaaaata aacgttcgta taataaaaaa ggagtttttt atggaaaaaa caaaacgtta
       61 tatcattgca acagcaggga tacttctcca cttaatgttg ggatcgacct atgcttggag
      121 tgtttatcgt aatccaattt tacaagagac cggttgggat caagctcctg tcgcttttgc
      181 cttttcttta gctatttttt gtttaggttt atctgctgct tttatgggaa atttagttga
      241 acaatatggt cctcgtctaa ccggaacggt gtcggctatt ctttatgctt ctggaaatat
      301 gctgactggt ttagcgattg atcgtaaaga aatttggctt ttatatattg gttatggtgt
      361 tattggtggc ttaggacttg gagcgggata tatcacaccg atatcaacta ttataaaatg
      421 gtttccagat aaaagaggca tggcaacagg gtttgctatt atggggtttg gttttgcgtc
      481 tttattaaca agtccgattg cccagtggct aattgaaaca gaaggtttgg tagcaacatt
      541 ttacctttta gggctgattt atctaatagt tatgcttttt gcttctcaat tgataataaa
      601 accaactgct gctgagatag ctatactaga taaaaaaaga ttacaaaata acagttattt
      661 aatcgaagga atgaccgcta aagaagcact taaaacaaaa tcattttatt gtctttgggt
      721 aattttattt atcaatatca catgtggctt aggacttatc tcagttgttg cgccaatggc
      781 acaagatcta actggtatga gtccagaaat gtcagcaatt gtggttggtg ctatggggat
      841 ttttaacggt ttcggtcgtt tagtgtgggc cagtttgtca gactatattg gacgccgtgt
      901 gacagtaata cttttatttt tagtaagcat aattatgact attagtttaa tatttgcaca
      961 tagttcccta attttcatga taagcattgc cacccttatg acttgttatg gtgcgggttt
     1021 ttctctcatt ccgccttatt taagtgatct ttttggggca aaggaactgg ctacattaca
     1081 tgggtatata ttgacagctt gggcaatagc tgcattaact ggacctatgt tattatctat
     1141 cactgttgaa tggactcata attatcttct taccttatgt gtttttattg tcttatacat
     1201 tttaggatta atggtagcat taaggttaaa gaaatgatgt cctataaaaa atttaaaagt
     1261 aaaccctagc aaagcctagg gtttttttat ataattaggt ttatggttaa atcatacagt
     1321 aaaacagcaa atcataatat gcgtcgtcct gttgtcaaag aagagcttgt tcattatatg
     1381 cgaacacggc agaagcaaat cacaggtttt ttagcagagt tagagcaatt cgcaagacaa
     1441 gaaaacattc ctattattca gcctgaggtc gtggcttatt tcaggttttt attgcaaagc
     1501 ttacagccaa aacacatttt ggagattggc acagccattg gtttttctgc gcttttaatg
     1561 gctgaaaatg caccagatgc gacgattgtg actattgatc gtaatcgaga gatgatcgat
     1621 tttgctaaag caaattttgc taagtacgac agtcgccaac agatccgcct gttagaagga
     1681 gatgcagcag atatattatc tacacttgaa ggaaattttg actttgtgtt tatggattcc
     1741 gccaaatcca aatatattgt ctttctacca gagattttaa gactgttaaa agtcggtggt
     1801 gtagtcattt tggatgatgt ctttcaagga ggtgacatta caaaaccgat cgaagacatt
     1861 cgacgaggtc agcgtactat ttatcgtggc ttacagtctt tgtttgatgc taccttaact
     1921 catccaaacc taacaactag cctggtccct cttagtgatg gattattaat gattcgtaaa
     1981 aatcaagcag atattctatt accagattaa gtaacattga taaatttatg ttataataaa
     2041 ttagttaaag ataaaaagga gctcagaagt aaatgaaaaa ctcaaataaa ctcattgcta
     2101 gtgttgtgac attggcctca gtgatggctt tagcagcttg tcaatcaact aatgacaata
     2161 ctaaggttat ttcgatgaaa ggtgatacaa ttagcgttag tgatttttac aatgaaacaa
     2221 agaacacaga agtatcgcaa aaagcgatgc taaatctggt aattagtcgt gtttttgaag
     2281 ctcaatatgg tgataaggtt tcaaaaaaag aagttgaaaa ggcgtatcat aaaacagctg
     2341 aacagtatgg cgcttcattc tctgctgctt tggcacaatc aagcttgaca cctgagactt
     2401 ttaagcgtca gatccgctct tcaaaattag tagaatatgc ggttaaagaa gcagctaaaa
     2461 aagaattgac aacacaagaa tataagaaag catatgaatc ttatactcca acaatggcag
     2521 tcgaaatgat tactttagat aatgaagaga cagctaaatc agtcttagag gaactaaaag
     2581 ccgaaggcgc agactttaca gctattgcta aagaaaaaac aacaacacct gagaaaaaag
     2641 tgacctataa atttgattca ggcgcgacaa atgtaccgac tgatgtcgta aaagcggctt
     2701 caagtttgaa tgagggtggc atatcagacg ttatctcggt tttagatcca acttcttatc
     2761 aaaagaagtt ttacattgtt aaggtgacta aaaaagcaga aaaaaaatca gattggcaag
     2821 aatataagaa acgtttgaaa gctatcatta tagctgaaaa atcaaaagat atgaatttcc
     2881 aaaacaaggt tattgcaaat gcattggata aagctaatgt aaaaattaaa gacaaagctt
     2941 ttgctaatat tttggcgcaa tatgcaaatc ttggtcaaaa aactaaagct gcaagtgaaa
     3001 gttcaacaac cagcgaatca tcaaaagctg cagaagagaa cccatcagaa tcagagcaaa
     3061 cacagacatc atcagctgaa gaaccaactg agactgaggc tcagacgcaa gagccagctg
     3121 cacaataagt aggattttat tgactaatca gttaaaaaaa cttataataa tttagttaag
     3181 aattgtttat caaggattag atacagaaaa gtgacggttg ctgcgagtca ttgataatta
     3241 gataaacatg ctacttaact ttacaacaat tgatatcgta aattaagaat gacaagttca
     3301 ttgaatagag gtggtaccgc ggttttcgcc ctctgtgaga tggacttgtt ttgtattgag
     3361 gactatttga aatcaacgaa aggaatagag tagactaggc tgccaggctt atagctgatc
     3421 tcctagcaag aaagacgagc tgtgtcaact ggtgtgatta cattactagc ttcagcataa
     3481 cactgttttt cttactcttt gtatctaaat ttttatgaaa gaattatcgt ctgcacaaat
     3541 ccgccaaatg tggttggatt tctggaaatc taaaggacat tgcgttgagc cttcagctaa
     3601 cttggttcct gtgaatgacc caacgcttct ttggatcaac tcaggtgttg caaccttgaa
     3661 aaaatacttt gatggttcag tgattccaga aaatccacgt attaccaatg cacaaaaatc
     3721 aattcgtact aatgatattg aaaatgttgg taaaacagca cgtcaccata ctatgtttga
     3781 aatgcttggt aacttctcaa ttggagacta tttccgtgat gaagctattg agtggggatt
     3841 tgaactcttg acaagtccag actggtttga tttccctaaa gataagctct acatgactta
     3901 ttacccagat gacaaggatt catataaccg ttggattgct tgtggcgttg aaccaagtca
     3961 cttggtgccg atcgaggata acttctggga aatcggtgct ggtccttcag gtccagatac
     4021 ggagattttc ttcgatcgtg gtgaagattt cgatccagaa aatatcggac ttcgcctctt
     4081 ggctgaagat atcgaaaacg atcgttacat cgaaatctgg aacatcgttc tctcacaatt
     4141 caatgctgac ccagccgtac cacgttcaga atacaaagaa ttaccaaaca aaaacattga
     4201 tacaggtgct ggtcttgaac gtcttgcagc tgttatgcaa ggggcaaaaa caaactttga
     4261 aactgacctc ttcatgccaa tcatccgtga agtagagaag ttgtcaggta aaacttataa
     4321 tccagatggc gacaacatga gtttcaaggt tatcgctgac cacatccgtg cgctttcatt
     4381 tgctatcggt gatggtgcgc ttcctggaaa tgaaggtcgt ggttacgttc ttcgtcgtct
     4441 tctccgtcgt gctgttatgc atggtcgtcg tcttggcatc aacgaaactt tcctttacaa
     4501 attggttccg actgttggac aaatcatgga aagctactac ccagaagtgc ttgaaaaacg
     4561 tgattttatc gagaaaatcg ttaaacgtga ggaagaaaca tttgctcgta ctatcgatgc
     4621 aggtagcggt cacttagatt cattgcttgc gcagcttaag gctgaaggta aggatactct
     4681 tgaaggtaaa gatatcttca aactttatga tacttatgga ttcccggttg aattgacaga
     4741 ggaattggca gaagatgcag gctacaagat tgatcacgaa ggctttaagt cagccatgaa
     4801 agaacaacaa gaccgtgcgc gtgcagctgt tgttaagggt ggttcaatgg ggatgcaaaa
     4861 tgaaacccta gctggtattg ttgaagaatc acgattcgaa tacgacacat atagtcttga
     4921 atcaagtctt tcagtcatca tcgctgataa tgaacgtacc gaagctgttt cagaaggtca
     4981 agctcttctt gtctttgctc aaacaccatt ctatgctgaa atgggtggac aggttgctga
     5041 cacaggtaga atcaaaaatg ataagggtga cacagttgct gaggttgttg atgttcaaaa
     5101 agcaccaaat ggtcaacctc tacacactgt aaacgttttg gcatcacttt cagttggaac
     5161 aaactacaca cttgaaatca acaaagagcg tcgtttggct gttgagaaaa accacacagc
     5221 aactcacttg cttcatgcag cccttcacaa tgttatcggt gaacacgcaa ctcaggctgg
     5281 ttcattgaac gaagaagaat tcttgcgctt tgattttact cactttgaag cagtaagcaa
     5341 tgaggaactt cgtcacattg aacaagaagt taatgagcaa atttggaacg ctcttacgat
     5401 cacaacgact gaaactgacg ttgaaaccgc aaaagagatg ggagcaatgg cactttttgg
     5461 tgagaaatat ggtaaagtgg ttcgtgtggt tcaaattggt aattattctg ttgaactttg
     5521 tggtggaact cacttaaata attcttcaga aatcggtctc ttcaagattg tcaaagaaga
     5581 aggtattggt tcaggcactc gtcgtattat tgcagttact ggtagacaag cttttgaagc
     5641 ttatcgtaac caagaggatg ccctaaaaga gatcgctgct actgtaaaag ctccgcaatt
     5701 gaaagatgca gcagctaaag tacaagctct tagcgactca cttcgtgatc ttcaaaaaga
     5761 aaatgcagag cttaaagaaa aagcagcagc tgcagcagct ggtgatgtct ttaaagatgt
     5821 tcaagaagct aagggcgtgc gcttcattgc tagtcaagtt gatgttgcag atgcaggggc
     5881 acttcgtaca tttgctgata actggaaaca aaaagactac tctgatgtgc ttgttctcgt
     5941 agcagctatt ggtgagaagg ttaatgtcct tgttgcaagc aaaactaaag atgtccacgc
     6001 tggtaacatg atcaaagaat tggcaccaat tgtagcaggt cgtggtggag gtaaaccaga
     6061 catggctatg gcaggtggta gcgatgcaag taaaattgca gagctgctag cagcagttgc
     6121 tgaaacagtg taaatccata aaaaaccaag ttatttagaa cttggttttt tatatttgaa
     6181 atgactacca tcataacctt tttgactatt atcgttgtca aaaagaaaaa gatatgttac
     6241 aattatagta acaaatagac atgaggaggt gttagagaaa atgacacatg gtatcaaact
     6301 taagaggcag atttgcttat gggcaagtat cctgactctt ttcattttaa gttatcatct
     6361 tatcagggta atctggtttg gagtaccctt gagttatctg gttttgggct ttacaggact
     6421 agccttgctg ttgaatactt tttcttattt tagaaagtaa gttataagtt aaagaagaat
     6481 gaaggagatg gcaatggagg ttattcttaa aaatcggctc aaagaattac gagccagaga
     6541 tggaattaat caaacggaaa tggctaaact ggcaggagta tcgaggcaga ccatcagtct
     6601 cattgagcgt aacgagtaca ccccatcagt tataattgcc atgaaaattg caaaggtatt
     6661 tcaggaacca gttgaagagg tgtttcgtct agtggaggtc gaggaatgaa aaagaagaag
     6721 aatagttttt tacgcttgtt aaaaatgtta ttgctatcaa gtttggcggg agggattata
     6781 ggtggcatgg ttggagcctt tttaggttac catggagaga gattagatca gctcacgttc
     6841 ctaaaagatg atgtgattaa cctgattatc ttattaaatc gtctagtagt tgtcacaggc
     6901 ctcacacttt cctttgtttt tttaacgcaa ctgaaaaaag agacagctgt ttataacaca
     6961 attgaagagg atgattattc tgaaaatggt taccgtcagc tgaataaaaa gcatgcttat
     7021 accatgttat tgattgccgt agcttctatt ttatccatgt gcaatgtgct acttggtctg
     7081 accttaacta atgattcaca gcatgccatg ctggccattc cgcttctgga tatcctttta
     7141 ttattaatgg tcattccttt tcaagcttta gccatgaaac gttacaatgc cattcgggga
     7201 accgatgttc cttattttcc aaatttgaaa gaattaaaac ataatatcat ggctctagat
     7261 gaggcagagt tacaagccta tcataagaca tcgtttgaaa gtgtgttgtc attaaatgga
     7321 gtcatcatcc cttccttgta tgtgatttta ttttttgtct atctattcac tggtcaagtg
     7381 gagttaacag ccatcttagt tttggtgtta attcagctct atttactcgt taagagtgca
     7441 acaatgacca gacaatttta ccgttagcct aaaaggagac tgtttatgaa aggattcatt
     7501 aattatttaa aaatagcagt gctcattatt ctggctatgg ttttcaatgt ccttccgatg
     7561 attttattgc aaaagcaaca cgatattcct atggtgctta attggggaat tggtattttc
     7621 tacttggtta ttgttggaag tgtccttatt gtattatggc gtctttatca agctaagcaa
     7681 gacaccccta ttaaacagca aaaaatgaga ttggttgact ggggttattt agcattattt
     7741 tggttaatca tccgtgtgat agctattgca ggtacccttg ttaaccagct atggtctggt
     7801 caacaagtga gtgctaatga tgctgcaata cataccttag ctagacttat caaaggtggt
     7861 ttcccgcttt atactgccct atttgtactt gtgatagctt ttatcgctcc tattatggaa
     7921 gaactagtct ttagaggatt tcctatgatt gatctcttca aaggaaaatc acttaaggtg
     7981 gcaggtttag tgacctctct tgtttttgct ttaccacatg ccaccaatag tgttgaattt
     8041 atcatgtaca gctgtatggg catttttctc tttgttgcct atcaaagacg aggaaactta
     8101 aaagacgcta tcttgttaca tatttttaat aacttgattg aagtcatttt gttaatgtca
     8161 ataggcttag gagtcatata ataaaaagct tcggatggat gaactgcacc ccaaaagttg
     8221 gacaaaaaaa tctaacattt gggttggaat tgtcaagaaa aaatagacat aaagttaagc
     8281 taatgagtaa ctattttttt caaattcaac aggtgagaga taccctaaag ctgaatgcag
     8341 acgtttgggg ttatagtaag tctcactata tttaaaaatc tcaagcttag cttgctcaat
     8401 agttgcgaaa tgagcatcat tgacaagctc tctttttaat gttttataaa aggattccat
     8461 gagtgcatta tcgtatggat taccttttcg actcatcctg gacttacatt ttttccatct
     8521 aagtaggtcc tgaaatcgtg ctccggtata ttgagaaccc tgatctgtat ggacgattac
     8581 cccttcttta ggcttttctc tattataagc ttgattgaaa gcttctgtta ccaacttgtc
     8641 ttgcatacgt tggcccattg cccaaccaac aacctttcta ctataaacgt ctatcaagac
     8701 agacacataa agtattcctt cttgtgtagg aatgtaggtt aggtctccta gccataattt
     8761 attcttacca gtcgcttgga aacattgatt aacaagattg ggacgagttc atgaagaacg
     8821 tcgacggtta tagtatttat attgatatcg acttccttta gcatagag
//
LOCUS       DATUYR010000058         8628 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.058, whole genome shotgun sequence.
ACCESSION   DATUYR010000058 DATUYR010000000
VERSION     DATUYR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.058"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(57..2633)
                     /locus_tag="VCI33_001383"
     CDS             complement(57..2633)
                     /locus_tag="VCI33_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEP2832302.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKINAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPPTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFPSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLSIESYLEAMNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGK
                     NLPVKKAQGGFLSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSK
                     LL"
     gene            complement(2716..4335)
                     /locus_tag="VCI33_001384"
     CDS             complement(2716..4335)
                     /locus_tag="VCI33_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP2832303.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVIVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(4414..5286)
                     /locus_tag="VCI33_001385"
     CDS             complement(4414..5286)
                     /locus_tag="VCI33_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP2832304.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    5409..5650
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5705..6727
                     /gene="trpS"
                     /locus_tag="VCI33_001386"
     CDS             5705..6727
                     /gene="trpS"
                     /locus_tag="VCI33_001386"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEP2832305.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
     gene            7035..8516
                     /gene="guaB"
                     /locus_tag="VCI33_001387"
     CDS             7035..8516
                     /gene="guaB"
                     /locus_tag="VCI33_001387"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEP2832306.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT         2896 a         1393 c         1634 g         2705 t
ORIGIN      
        1 gcttttagta tattgactca tttgccaaat tgataaaaaa atctgatttt tttggactaa
       61 agtaatttag actttatatc tatgtactta tacaaaagca tataagcgat aattcctacg
      121 caagatagag ataaccctaa tttaaaacca ttaggaataa aggtaaggat aacacgtccc
      181 tttcctttag gaatagtaac tgatagaaat cctccttgtg cttttttgac tggaagattt
      241 ttcccatctt tttgtgctga ccaaccttta tcgtaaggaa gtgtaaaaat aagagaacct
      301 ttcgtttttg aattataatc agtgattacc gtattacttt tagcgtaagt atgaacattt
      361 ttttgtttaa tactattcat tgcttcaagg taagattcaa tagacaaact ataaaaatga
      421 ggttccttaa aactaatttg tttatttttt ggaaaaacaa acgaaaatgt agcaactttg
      481 gcatcggcga aatatcctaa atcaaaaaaa gagtaagcgt tatctgtagt atatatgaaa
      541 ttatgattat ctgtctgaat acgcacctct ttagcatcag gatttgaaaa tataatattg
      601 ggtatgctaa catagagttg actatgttta gggatgttaa tttgataatt aagggtaacg
      661 gagttttgat aatcagaagc ttgtgcagat atttgtccgt taaattgatt agcaccagaa
      721 ataagttgag ctggctgtaa gttaaaatag gttaaagatt tcccacttag ttggttaagt
      781 aatttggttt ggttatcaag ggtattgaca tttagattga catctttgta aacattacgt
      841 gttaatatag ctaaagggct actatagtga ttttgataaa gagtagtatt cccgctagtt
      901 tttagttttg taaatccaaa tttatttaga ggtccttcgc taagattata ttttatacca
      961 agtagactgt ccataataat agtattgttt tggtatctaa ggtttaaatt cgtgccttta
     1021 gactgaaatc ccaatcgatc caataaagaa ctagatagtc tatttcttac agaggaaaat
     1081 tgtgaaatgc cgtaataatt aaatttcatg ctatcgttcc ctgtttgggg aagtagtctt
     1141 tccattctaa aaaaaggttg actatttttt gacacagagt tgacaaggtt gttaatatcc
     1201 tttaattgac tattatatat ctgtcgtgat gggaatcccc actccttatt aattccttga
     1261 agctggtagt aggtgtttaa ccctgattca agaaggctaa agataagtat gaaagcagaa
     1321 ataaaagtag gtgggatttg agaatttcta aacgaaaata gtgaaatagt ataacctaat
     1381 aataaaaaaa cactaagtaa aaaaagagtt aaaggtagaa aattatattg ttgagaaaag
     1441 ctataaggaa gagatgtcag tataatgagg aaaataaaag caaaacctgc ttttatttga
     1501 gtcacttctt ttagtcgaga gagagtctca catgcgagta ataggataac tatggaaaaa
     1561 gaccaagcgt agcgatgcaa aaacatattt ggtgagtgca tcccctgcca aaaaagatca
     1621 agtggctgta ggtaaaaact tattataata aaagttaaca agcaggcatt ggctaatttt
     1681 atttttaaag ggatactttc taaagtaaaa taaataacac taagcattag gggaaataat
     1741 cctacgtaaa tcataggcag agcattaaac ttggtagtat cgtacactcc tattgagagc
     1801 ttagcaggta tatccaaaaa ccaagcattg ttcgtaacta actgttttac cggggataga
     1861 ttctctccat aagttgacaa atctagatag gtaggaagta ttaccagagc acttgttaaa
     1921 gcagcacata ttgacacagc tgtaaaccta acaaaagcga taaacatttt gttaaaatca
     1981 ttgagactta aaagacaaac taaggcgtaa agaatacaaa aaagagcaat catgtagcca
     2041 aagtagtaat tttgaatgaa taataatgat atcgaaagat aataagttct ggttttattt
     2101 tctgtgataa gtttatttaa tccaagtaca acaagtggaa gaagaatgaa aacatctaac
     2161 caagaattta gttccatttg acttgtcaag aagctcatta agctataaaa aactgagatg
     2221 gaaatcatca agaaagcatt gatttttgga tataatctat gaaaagaata gcatgcagct
     2281 aatcctatta acccaaattt tatcaaggta aacaaataga tagcatctgg catagaggtt
     2341 aaattaaaaa agaaaagtaa tggagaaaag aaactgccaa gataataaca cattaaagca
     2401 taaaaattta tccctagtcc gcttgtaaag gtataaaaaa aactatcaga accgtgcatg
     2461 atgttacgaa agttctgcgc aaaaataaca tactgatgaa aagcatcact agctagaatt
     2521 gttttatcac tattataata aatgcccatc gatagaagga tgataaatat aatactaaga
     2581 gggaacaaaa aactagcaag tccagcaatt atccatttat tattattttt cataaattat
     2641 atggtctttc gtttacaaat tttaaaaaag aggctaggaa gagatcttct tagccacctc
     2701 atttatcata aggttttatt tccaaagttc agcaactcgg gcttggactt ctggattgtc
     2761 aaggaattcg tcataagttt catctatacg gtcgataaca ccattttttg aaataacaac
     2821 gatgtggtta gcaatcgttt gaataaattc atggtcatga ctggcaaaga taacagattc
     2881 tttgaagtct ttgatcccat cattaagact tgaaatagat tccaaatcca agtgatttgt
     2941 tggatcatca agaataagaa cgtttgattt caatagcatc aacttagaaa gcatgacacg
     3001 cactttttcg ccaccggaaa gaacgataac agatttttta acttcgtctc cagagaaaag
     3061 catccgtcct aggaaaccac gtaaaaaagt atcgtcatct tcaccttttg aggcaaattg
     3121 acgtaaccat tcaagaatag attcttccgt ggcaaaatct tttgaattgt ctttaggcaa
     3181 gtaagaacga cttgttgtca ctccccattt aatggttcct tcgtagtcaa tatcatctgc
     3241 taaagcacgc ataagagcgg ttgtttgaat atcattttga ccaataatag cagctttgtc
     3301 acctggacgt aaaatgaaac tgatattatc aatgattttc tccccatcga tcgttacaga
     3361 aagattttca acagtgagga agtcattccc catttcacgc tctgctttaa aattaataaa
     3421 tgggtattta cggcttgagg gaacaatttc ttctaattca atcttatcaa gcattttttt
     3481 acgagaggtt gcttgttttg atttagatgc gtttgctgag aaacgggcaa cgaattcttg
     3541 taattctttg attttttctt cggctttagc attgcggtca gcttgtaaac gagcggctag
     3601 ctcagaagat tgtttccaga aatcgtagtt acccacaaag agtttgattt taccaaagtc
     3661 aaggtcggcc atgtgagtac atactttatt caagaagtga cggtcatggg aaacaacgat
     3721 gactgtattt tcaaaatcaa tcaagaaatc ttctaaccat gaaatagatt gaatatcaag
     3781 accattggtt ggctcgtcaa gtaataaaac gtcaggctta ccaaagagcg ctttagcaag
     3841 aaggactttc actttatctc cattagccag ttcactcata ttttggtagt gaaggtcttc
     3901 tggaatattt agattttgga gaagttgaga agcttcactt tctgcctccc agccacctaa
     3961 ttcagcaaag ataccttcta gctcagcggc acgaacccca tcttcttctg aaaaatctgc
     4021 tttcatatag atagcgtctt tttctttcat gatattgtaa agttgctcat tacccatgat
     4081 tacaacatca atcgcacgtt cttcttcata atcaaaatga ttttggcgaa gaacagataa
     4141 gcgttcgtca ggtcctaaag agatatgacc agtacttggt tcaatatctc ctgcaaggat
     4201 tttaagaaaa gttgattttc cggcaccatt cgcaccgatt aagccgtaag tattgccggc
     4261 tgtgaatttg atattaacat catcaaatag ttttcgatca ctaaagcgta gtgatacatc
     4321 agaaactgta agcaatttgt cacctcattt tatttatcta agcttcattg tacctaaaat
     4381 agtccccttt ttcaatcaga aaagggctaa tcactacata ggcaataaaa cgtctttgtc
     4441 ttcacgtttg tattgtttag tcaaactaaa tcctcttccc ataacttgag ctgctggcat
     4501 gacaacaata aaggctgtgt cgtcaatttc gaggattttt tcttggagag tgggtaattc
     4561 gtgacttgat acaatagcca ttaacataat tttatcagaa gtagtataac caccgcgaat
     4621 aggaagttta gtcactccac gatccataac agttgtgata tattctcgaa tagcttgata
     4681 atctttggaa ataatcataa catttttgga tgagtcaaag ccattttcca taacgctaat
     4741 cacataacca attacgaaaa gtccaatcgt agaatacatc acgtcatctg cagaaagtgc
     4801 aataaatccc ataaggacac taataccatc tacaattgtc attgcaactc caagagataa
     4861 aggagagtat ttatgaagta tttgtgttag aattccagtt cctcctgtcg aagagtttcc
     4921 ccaaaaaacc attcctaaac caatgccaca gataatacca ccaaaaatag ctgctaacaa
     4981 ctgattgtgt gtcagagtag gtaatgaatt ggttgaacgg atggcaatag gatatatcca
     5041 agaaccataa agtgttttaa taaaattttg ttttccaaga aagaagtaac acattaataa
     5101 taagggaata ttactagcca ttaaaaaaag tgacggacta atgccaaata gagcttttat
     5161 aacaactgca ataccaacca taccccctga tgcaatatga ttgtcgacaa acattgtatt
     5221 gaaaccaata gctgttatga atgagccaat tgtgacaagt agtaaatcaa tccctcgttt
     5281 tttcatgctt ttaatctcct ttataactgg ttaaaaatag tagactcatt atctcttaaa
     5341 tgtactgaga ccgtcaagtc atattttcaa aaaatctggt gaacttgaca agaagtttaa
     5401 aatttcatag aataaatttg aattagaaga gtattctttt catgttttta gagagttagt
     5461 ggttggtgca agctaacaac agaaaaggtg aaatggacta atgattattt gaaaggaaac
     5521 cataactttc aggttggcac cccttaccgt gcagcttctt tcattgagaa gattgagata
     5581 gtatctcgtt ttgataaagc agatgctaat tgaggtggta ccgcgtatta cttgtaataa
     5641 cgccctcacg ttttaatagc gtggggactt tttgctatat caagagatag ttagggagaa
     5701 aatgatgaca aaacctatta ttttaactgg agatagacca actggaaaat tacatttagg
     5761 tcattatgtc ggaagtctta aaaatcgtgt ctttttacaa aatgaaaaca agtataagat
     5821 gtttgttttc ttggccgatc aacaggcact gacggatcat gctaaagaat ccgaattaat
     5881 tcaagaatct attggaaatg ttgccttgga ttacctctct gtcggtttgg atccgaagca
     5941 atcaacgatt tttattcaaa gtcagattcc agagctagct gaattgagca tgtattatat
     6001 gaatctggta tcattagcac gtttggaaag aaatcccact gttaaaaccg aaattgctca
     6061 gaaaggtttt ggcgaaagta ttccatctgg ttttttggtt tatccagtat cacaggccgc
     6121 tgatattaca gcatttaaag cgaatttagt acctgtaggt aacgatcaga aaccgatgat
     6181 tgaacaaaca cgtgaaattg tgagaagttt taatcatact taccatacag actgtttagt
     6241 agaacctgaa ggtatttatc cagaaaatga aaaagctgga cgcttacctg gtcttgatgg
     6301 caatgccaaa atgtctaaat cattgggaaa tggaatctat ctttcagatg atgcagatac
     6361 cgttcgcaaa aaagtgatga gcatgtatac tgatccaaat catattaaaa tagaagatcc
     6421 tggtcaaatt gaagggaata tggtctttca ttatttggat atttttgcta gaaaagaaga
     6481 tcaagctgat atcgaagcaa tgaaagagca ttatcaaaga ggtggtttag gagatgtgaa
     6541 aacgaaacgc taccttctag atattttaga acgtgaatta gcacctattc gtgaaagacg
     6601 tttggagtac gctaaagata tgggagaggt gttccgtatg ttacaagaag gtagtcaaaa
     6661 agcaagaact gtggcagcca agactttatc agaagtgaag tcagcaatgg gtattaatta
     6721 tttttaatgt tttagagaaa aagctaagtt agtaatgata agataattac tattatcaaa
     6781 gcaggaccta atttgtcatt tctttacagg atagtagaga ttatgtttta tatagtctta
     6841 tttttgatta aaaataagac ccaaatgaat tttttgatta ataaaaaacg gataatacta
     6901 atatattatg ctgatagtga cttcagctgt aggaatattt tattgacaaa tgattttagc
     6961 gtgatatgat agtaacaata aaaaatagac cagggtaata ttccctaaaa ataaaagaaa
     7021 agaggattgt ctggatgtca aattgggaca ctaaattttt gaaaaaaggc tacacttttg
     7081 acgatgtctt gcttattccg gcagagagtc atgttcttcc aaatgaagtt gatttaaaga
     7141 caaaactcgc agacaatttg acattaaata ttcctattat tacagcagct atggatacgg
     7201 ttactggtag taaaatggct attgcaattg cgcgtgcggg tggtttaggt gttattcata
     7261 aaaacatgtc tattactgag caagcagaag aagttcgcaa ggtaaaacgt tctgaaaatg
     7321 gtgtcatcat tgatccattc ttcttaacac ctgaacacaa ggtttcagaa gctgaagaat
     7381 taatgcagcg ctaccgtatc agtggtgtgc caattgttga aactttagca aaccgtaagt
     7441 tggtcggtat cattactaat cgtgatatgc gttttatttc tgattacaat gcaccaatct
     7501 ctgaacatat gactagtgag cacctcgtta cggctgctgt tggtacagat cttgaaactg
     7561 cagaacgtat tcttcatgaa caccgtattg aaaaacttcc tttggttgat aatagcggtc
     7621 gtctgtctgg tttaatcact atcaaagata ttgaaaaagt aatcgaattc ccacatgctg
     7681 ctaaagatga atttggtcgt cttctggtcg ctgctgcggt aggtgttacc tcagatactt
     7741 ttgagcgtgc cgaggccttg tttgaagctg gtgcagatgc gattgttatt gacacagctc
     7801 atggtcattc ggctggtgtt cttcgtaaaa ttgctgagat tcgtgctcat ttcccaaatc
     7861 gtactttgat tgctggaaat attgccactg ctgaaggtgc gcgtgctctt tatgatgcag
     7921 gtgttgatgt tgttaaagtg ggtattggtc ccggatctat ctgtaccact cgtgtggttg
     7981 caggtgtcgg tgttcctcaa gtgactgcta tttacgatgc tgctgctgtt gctcgtgaat
     8041 atggcaaaac gattattgct gatggtggta tcaagtattc tggcgatatt gttaaggctc
     8101 ttgcagccgg cggtaatgct gttatgcttg gatcaatgtt tgcaggtact gatgaagctc
     8161 caggtgaaac tgaaatctac caaggccgta agttcaaaac ttatcgtgga atgggttcca
     8221 ttgcagctat gaagaaagga tcaagcgatc gttacttcca aggttccgta aatgaagcta
     8281 ataaactagt tccagaaggt attgaaggcc gtgttgctta taaaggagct gcttcagata
     8341 ttgtcttcca aatgttaggt ggtatccgct caggtatggg ctatgttggc gcaggtgaca
     8401 ttcaagaatt acatgaaaat gctcaatttg ttgaaatgtc aggtgcaggc ttgattgaaa
     8461 gtcatccgca tgatgtccaa atcacaaatg aagcaccaaa ctattctgtg cactgataat
     8521 ttaatttagt aactttaagg gctgacatat tgtgccagct ctttttttat attaaaaaat
     8581 gataaaatat aataacaatt ttcggataac ttttattgtt ttttctta
//
LOCUS       DATUYR010000059         8506 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.059, whole genome shotgun sequence.
ACCESSION   DATUYR010000059 DATUYR010000000
VERSION     DATUYR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.059"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(355..1761)
                     /gene="lacG"
                     /locus_tag="VCI33_001388"
     CDS             complement(355..1761)
                     /gene="lacG"
                     /locus_tag="VCI33_001388"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEP2832307.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(1834..3531)
                     /locus_tag="VCI33_001389"
     CDS             complement(1834..3531)
                     /locus_tag="VCI33_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP2832308.1"
                     /translation="MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMQVILFSSIF
                     ILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKVYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEK"
     gene            complement(3531..3848)
                     /locus_tag="VCI33_001390"
     CDS             complement(3531..3848)
                     /locus_tag="VCI33_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP2832309.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(3872..4855)
                     /gene="lacD"
                     /locus_tag="VCI33_001391"
     CDS             complement(3872..4855)
                     /gene="lacD"
                     /locus_tag="VCI33_001391"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP2832310.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(4858..5787)
                     /locus_tag="VCI33_001392"
     CDS             complement(4858..5787)
                     /locus_tag="VCI33_001392"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEP2832311.1"
                     /translation="MILTVTLNPAIDVSYPLNELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNDFGETVKATGCIGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVDEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            complement(5833..6348)
                     /gene="lacB"
                     /locus_tag="VCI33_001393"
     CDS             complement(5833..6348)
                     /gene="lacB"
                     /locus_tag="VCI33_001393"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP2832312.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHEIIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRAALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(6383..6811)
                     /gene="lacA"
                     /locus_tag="VCI33_001394"
     CDS             complement(6383..6811)
                     /gene="lacA"
                     /locus_tag="VCI33_001394"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP2832313.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            7257..8030
                     /locus_tag="VCI33_001395"
     CDS             7257..8030
                     /locus_tag="VCI33_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP2832314.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
BASE COUNT         2646 a         1662 c         1398 g         2800 t
ORIGIN      
        1 aacaaatagc cctctgatat aaaaaacaaa actcgttctt tacagctatt ttacaatcta
       61 atcgcttctt gttataacat caatctctaa tcagaaaatg gttactttac ttatttaatc
      121 aggtcaggag tttgaatgac agaagtcaaa tatctgaaaa aggatattaa ataaaacttt
      181 caaaacccat tttcatcaac taaaaacact tataacccga tcttacaact aatggattac
      241 catcaacagc aaataatact atgcttgaaa aatgtcaaca tcatctactt tttgtgacta
      301 taattcagta aacgaacatg aagtgtaaaa atgtattaaa aaagtatctc aaaatcattc
      361 aatcacttga gtttctgcta cttttttata ccagtaggca ctcttcttag gataacgttc
      421 ttgagtttca aaatcgacat agaagagacc gtaacgtttt tcatagccat ttgaccatga
      481 aaagacatcc atcagtgacc acataaagta tcctttaaca tttgcaccat cagaaatagc
      541 atcagaaata acttctaagt gttttttcac ataatcgata cgtccatcat catagacagt
      601 attatctaca aactcatctt tgtagccaag accattctct gtaatgtaaa ttttcttgta
      661 gttaggataa tcggctttga cacgcatgat ttgatcatat aagccttgtg ggaagataat
      721 ccagtcccag tccgtttttg gaacatcaac gggtgctttt cttcgaccaa cacccttgat
      781 ttggtattta gagctgcctt tttccccttt accattatga atgatttcag tctcaccatc
      841 aaaagcttgc atccaatcac tcatataata gttaatacca aggaagtcat tcaaatcttt
      901 tgcagcgtct agtgcagcaa aatcttcctc acgaagatca agttcaccac catttacctc
      961 aaggatatgg ttaacacctt ccattgtttt atctgaatac ttacctagat aagttgcatc
     1021 aagaataaat ttattatgga tgatatcttc cagttcagct gctctaacat catcaggatt
     1081 gttagcgtca aatggatact tagttggaag agcatggacc acgccgattt cacctgaata
     1141 gccactatct ttaaagagtt tgactgcacg agcatgagag accatcatgt tatggtgtga
     1201 ttggaaaact ttagcaagat catattggat acctggaggg aatttaccaa ccaagtattg
     1261 gccatcacca ataggcccaa tttcgttaaa tgttgtccaa tagttaactt ctgagaattc
     1321 tttaaaacaa aattctgcat aatttacaaa atgttcaatg ttctcacgat tgaggaaatc
     1381 accatccgag tggagagctt ctggggtatc aaaatggtga agtgtaacaa aaggctcaac
     1441 atgacgctta tgacactctg caaagagatt gtggtagtat tcaactcctt tagggttaac
     1501 ttctcctttt cctgttggaa aaatacgaga ccaggcaata gagatacgga tgccgttgac
     1561 accaaattct tcactaagtt tcaaatcgac tggataacga ttgtaaaaat cacttgctgg
     1621 ctcagctgtg taccaatagt tgtcttctaa gtatttatcc caagctactg gtcctttacc
     1681 atctgtgtgg gtagcgcctt cagcctggta agcagctgta gcaccaccaa aaataaaatc
     1741 tttaggtaat gttttagtca tgatatacct cttctaaatt ttcatttcac tataaaggaa
     1801 gattgagtac ccccaacctt ccctagcgac atattatttt tccatttgtt ctttaacgaa
     1861 gtctaatgcg ccttgaccat cgcgagtcaa tttgatgtat tgagcccctt ctgttttagc
     1921 taatttaata tcaagtttat cagtttccac tttcatatct tcatagttag aagctacttg
     1981 aggtgcaagg ataacaagtt gatattgtgg taaaatctca cggtgagcac cataacttcc
     2041 tgctgctgca gtgacaggtg caccatattc tttagctgct ttattcaaag cgtttgcaag
     2101 caaaccactt gtaccaccac cggcacaaag aacgagaaca ttagtttgat cagtgatttc
     2161 tttggtttct ttagctgctg atttttcaag aatagcatca gcttttttag tatcaaagtt
     2221 tgctgctact ttttctttga gggcatctga tgaattcgta cctgctgctt cttcagcaag
     2281 aatttgctca tcatatactt ttaagaatgg gtagtagata atcacatcaa caacaattaa
     2341 caaagctgcc aaaatgaatg ctagcacttg gaagtttgtc ccaaggataa gacccaatgg
     2401 agctggtgtt gtccaaggta agtttgcagt gaaactgttc attccaagtg tgtcaatgaa
     2461 gaatttgaaa atccatacgt tgacaatcgg tgccaggata aatggaataa agaagattgg
     2521 gttcaaaaca agtggggcac caaacaaaat gggttcgtta acaccaaaga atgttggaac
     2581 aactgatgca cgtccaatgg ctttgttacg ttttgatttt gttaaccaca tgaacatgaa
     2641 tggaacgacg agagttgcac cagtaccacc catggtaacg atgaacattt gagtccctga
     2701 agtcaagatc ttatcagcat gttcaccagc ttgaagtaat tgaaggttgg tttcagcatt
     2761 tgcgtaagta atcgctgcaa tggctggttc aacaatagat ggtccatgaa taccaacaaa
     2821 ccagaagaaa gcaaatgcac cgaagataat agtgattcca acatagccat ctgctgcttg
     2881 gaacaatggt gcaagaagtt taccaattga ttctgcaacg ttaacgccta aagtagtatg
     2941 aacaatcaat tcaatcacat aaagcagtac aactgataac gtaaatggaa tcaaatcttt
     3001 aaaaacttga gatatatttg gtggaacctc ttctggcatg cgaatagtaa cattgttttt
     3061 cacacaggtt ttatagacat ttacggtaat aaaagctgcg atgaaggctg tcaacaaacc
     3121 ttttgttccc ataaatccag ttaagaaacc accgtctttt gctggattgg ctgccataag
     3181 taaaaaacct acaatagcag cgagcatagt tgaaataaag ttgatttggt ttgtgcttgg
     3241 taatgatcga ttcattgagt cagttaaagc tttggctgtc gtaccagcca caaacaaacc
     3301 taaaataccc attgagtagt tgtatggcgt catcaagaga ttctcaatat ccttactcca
     3361 atggaagccc catgcatttg gaacataggc aatcaagata aagatacttg aaaacaagat
     3421 aacttgcata ccagcgataa aaccatcacg aatagcgcga agatagatat ttcgagaaat
     3481 tttctcgaag aaaggtttcc ctttctcaat ctgagcaatt aatccattca ttattttgct
     3541 cctctcttat aaagttcaat catgtgtttc attaaatctt gtaataaaat ggtcgtcatt
     3601 aaatgatctt gaccatgcat taaagtgaca ctataagcta agtcatctcc agctgcttct
     3661 ttttgtagta gacttgtctg agcattgtga gcatcattca agcagtcact acctgcagcg
     3721 attaattctt ctgctttagc ataatctcct tcttgcgctg ctttaagcgc ttccaaaaat
     3781 tttgaacggg catcaccagc ataggctacg atttcaaaac ctaaaagagt agtttcttct
     3841 ctattcattg tagtttcctc cgaaaataat gttacatttt ttcagtccaa ggacttgcag
     3901 ttttatctag tactttgttt aattcgtcaa tatttttgaa accttctgta cgcaaccatt
     3961 cacgagctgc ttgtggtcct tcttcgatat agacttttac agatcctgcc caggtagcac
     4021 gtccacataa gacaccgttg aatttagcac ctgattctgc tgcaaacacc aaagtatctt
     4081 ggaacaattt ggcagaaaca cctgcactca agtaaatata tggtaaatca gtagaagctt
     4141 cttggtcacg gaaagcttga gctgcttctt cttttgtaaa gagaacctca ccatctgcaa
     4201 agctttctac aaatttcata tttactggaa cttcgacttt caagacgtca acaccaaagc
     4261 gttcttctga gaagactttc attgcttcat taactttatg agctttaact tttgcaaact
     4321 cagggcttgc attgtcagca attttctcat cataagttag aatttctaag tagaatggaa
     4381 tatcttctgc acggcattca gaaccaatac gttcaatgta agcttttttc tgctcgttaa
     4441 catcttgatc tccatcaata tcatagtaaa gaaggaattt aactgcttct gctccagctt
     4501 ctttaatacg ttttgcagac caaacatcta agcaatcagg taagcgacta gttgttgttg
     4561 catcgtatcc tgttttctca tatgccaaca acaagcctgc ttcttctgaa cgaacacgac
     4621 ttgctggcaa gccatattca ggatcaagta aaattgatga tgcaaatggt gtcagttctt
     4681 cagaaactaa acttttaagc tcttcaattt gttctactgt tggctctttt gtttgatgtt
     4741 gagccatcat acgtttgaga gctccacgtt ggtcaaaagc taaagcagaa ataacaccat
     4801 ctatacttag tttttccatg cttttacgtt tgttttcagt taaagtaatt gtcattttta
     4861 tacctctttg actttaatag attgatataa cttatcgtaa tttgccatgt taacatgccc
     4921 agttgttttt tcttgcgcat ttaacatacc taagacattt gcttttgtta ataaagctcg
     4981 atcatcttca tcattcgcta aacctgaagc aatacctgcc actgtagaat cacccgaacc
     5041 tacagcgctt acaattttga ttttaggaat gtcaacgtta taaaacgtat ccttgtgctt
     5101 tgcaaataca ccatcagcac caagagaaac gattatccat tcaattcctt caaatagttc
     5161 atcttgtaag acttctttta aggcttcaaa atcttttgtc atttctttac ctagaagttg
     5221 agaaagttct tcaagattag gtttgataac tgttggttta ctatcacctt tcaatactgc
     5281 ttctaaagca tttccagaac aatccagaac agtcttctta ccattaagat tggcaatctt
     5341 gattaacttc tgatagtaat catctggcat accagcaggc aaactgcctg agagcgtcac
     5401 tacgtcaaca tcattcagta aatatttaaa atgatgagta aatccatcaa tttcatctac
     5461 acttaacagc ggtccttttt ctaaaatctc agtttgatta ccttcgtgta atatggctat
     5521 acatgttctg gtatcacctg aaattttata gaagcgactt aaaatcgaat caggaagatg
     5581 attaatgata aaatcaccgc tttctccacc aatacaacca gtagctttga ctgtctcacc
     5641 aaagtcattt agaactcggg aaacattcaa gcctttacca ccgggtgtct ttgtaacgtc
     5701 tacaactcga ttgacagtat cgcattttag ctcattcaaa gggtaggaga cgtcaatagc
     5761 tggatttaaa gtaactgtta aaatcacaat agctcctttt tctatatatc cttatcataa
     5821 ttctttcaaa tattagtcgt gatattcacc acggtcccat ttttctagaa attcatcaaa
     5881 gaagtgtggg ttgttttgat cttggctagc atgttgcaag ctatccattt tttcaatcca
     5941 ttttttattt tcttcagttg gtttgtattc agccgctaag aaagcatcga taatatcatt
     6001 catcagcaaa ccaccgatga tttttccacc aaaaccaata acgtttgcgt ttagctcttc
     6061 ttttgagtag atagcagatg tcatatctct aacgagtgct gcacgaatac cgggaacttt
     6121 gtttacagca tttgtgatac caacaccagt accacagata acaacaccta attctgcttc
     6181 accacttgct actgcttcac caactttttt accaaagatt ggatagtgtg tgcgaacatt
     6241 atcgtatgta ccgcaatcaa tgatttcatg accttgtgat tttaggtaat ccacaacagc
     6301 gattttttca taagtaacaa tatggtcaca acctacagca attttcattt ttgttctcct
     6361 tgttttaacc tatttcaatc aattagcaca ttttattaag catgtcgaca cgaacttgat
     6421 gacgtccacc atcatattta ccttcgacaa agcctttgat aatgttcttt gcaattcctg
     6481 gagcactaat ttcagaacca agcgtgatga ttcgagcatt gttgtgtcct cttgtcatgt
     6541 atgctgaacg ctcatctgat acttcagctg ccaccattcc tttgatttta gtagctacca
     6601 tgaaagaacc aacaccatat gcatcaatga caatacctaa gttatcttcg gaggcattaa
     6661 cttcttttgc aacggctaaa gttgtatcta caaagtcacg tacttcgttt tcactaacat
     6721 caacgacttc gtatttacct tctttaaggt aatctttgat aacttcttta agttcttgac
     6781 ctgctttgtc agcaccgata ataattgcca ttttatttct cctatgattt agaataagta
     6841 aatgtggaaa acctacgtag atagttaatt aacgatttgc aacatctttt gaaataacgc
     6901 tttcattatt tctatgacct aatcatacac ccaccaaaca taaaagtcaa catttttttg
     6961 tttattttta taaaaaataa acaaaatatg tttatttttt tcatttttac gttattatcc
     7021 taatgtttct tgtgtttctt tttcatttca taaatccctt tgacagccac tcgtaaccta
     7081 tcatatatta cgagaccctt atctctattt attgtaaacg ttttatttaa aacattttct
     7141 attttgttaa tttttgtttt ttccaatgtt tatgtttgtt ttgtttgatt tttttattga
     7201 tattatgtta tgataccttt aatagttata ccctaagaca ctgataggag ataagaatga
     7261 aaaagaaaga gcgtcatgaa aaaattctcg atatcttaaa agttgacggt tttataaaag
     7321 taaaagatat tattgatgaa atgaacatct cagatatgac cgctagacga gatctcgata
     7381 cattggcaga taaaggatta cttatcagga cccatggtgg tgctcaatat ttggattatt
     7441 cttcagcaaa ggacgaaggt cacgagaaaa cacacactga aaagaaggtc ttacagacta
     7501 ccgaaaaaaa acaaattgca caaagagcta aaaacattat taaagacggc gaaactgtct
     7561 ttataggtcc agggacaaca ttagagcatc tagctgtcga attaaaaaat agaaatatca
     7621 gggtcattac aaatagcctt cctgtttttt taattttgca cccgagcact actattgatt
     7681 tgattttaat tggtggtgaa tgtcgtgaaa ttactggagc ttttgttggt gctattgcca
     7741 ttaataatct agaatcatta actttttcaa aggcttttgt tagcgcaaat gcagtttaca
     7801 ataattctat tgcgacgtac agtgatctcg aaggagaagt tcaaagggtt gcattgaata
     7861 atgctgttga gaaaatcctt ctagttgata gtaccaagtt tgaaagttat gacttctata
     7921 atttttacca actagatcaa gtggacttaa ttgtgactga taaaaatgtg acaactgata
     7981 tcatagaaaa gtacaagaaa ttctcaaaaa tcataatcgc tgacaagtaa aggacctact
     8041 catattaaaa agcacttcag ggtttagata tatcccctgt caagtaaaca gggagcatat
     8101 ctgaaagtag tgtttgtgtt ataaagaagt ttcttgctaa cttattttct ggtttttttc
     8161 agacttatca gtcttgtact gttccttttt ctgatgaata acgtggaaat aggcagacag
     8221 ctatatattt cacctctaga atggttgtca ttactgtcat caagacatgc ttgttcttta
     8281 tcgtatggag tgaagctttc ttttagacat caacatacct cttaagtaga ttttggttcg
     8341 ttaccatgtc tacttaagag gtgtcttcaa agcgctttaa aacgttgata taacagtata
     8401 ttaacgtttt gagaattgtg aagctttacg agctttttta agacctggtt tttggttcac
     8461 tccattattt aaagtcaaat ccctttaaaa tcaatgtttc taaaat
//
LOCUS       DATUYR010000060         8014 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.060, whole genome shotgun sequence.
ACCESSION   DATUYR010000060 DATUYR010000000
VERSION     DATUYR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.060"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            62..928
                     /locus_tag="VCI33_001396"
     CDS             62..928
                     /locus_tag="VCI33_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEP2832315.1"
                     /translation="MKMKKKFFLLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            939..1874
                     /gene="pstC"
                     /locus_tag="VCI33_001397"
     CDS             939..1874
                     /gene="pstC"
                     /locus_tag="VCI33_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEP2832316.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATCWQTIWRVVLNAARP
                     GIFTAIIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            1864..2751
                     /gene="pstA"
                     /locus_tag="VCI33_001398"
     CDS             1864..2751
                     /gene="pstA"
                     /locus_tag="VCI33_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEP2832317.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGSLALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            2767..3570
                     /gene="pstB"
                     /locus_tag="VCI33_001399"
     CDS             2767..3570
                     /gene="pstB"
                     /locus_tag="VCI33_001399"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP2832318.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            3583..4341
                     /gene="pstB"
                     /locus_tag="VCI33_001400"
     CDS             3583..4341
                     /gene="pstB"
                     /locus_tag="VCI33_001400"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP2832319.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            4409..5062
                     /gene="phoU"
                     /locus_tag="VCI33_001401"
     CDS             4409..5062
                     /gene="phoU"
                     /locus_tag="VCI33_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEP2832320.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            5267..7804
                     /locus_tag="VCI33_001402"
     CDS             5267..7804
                     /locus_tag="VCI33_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEP2832321.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
BASE COUNT         2429 a         1403 c         1641 g         2541 t
ORIGIN      
        1 gaatatgtgg cacttagttt ctacatatat accctttatt tttacgaata ggaaataaat
       61 aatgaaaatg aaaaaaaaat tctttttgtt aagtcttttg gtcctatcaa ctttcttttt
      121 atccgcatgt tctagctgga ttgataaagg tgagtcaata accgctgtag gatcaacagc
      181 actacaaccc ttagtagaag cagtagctga tgaatttgga agcagtaatc taggcaagac
      241 tgtcaatgtt caaggtggtg gttcaggtac agggttgtct caagttcaat caggagctgt
      301 ccaaattgga aatagtgatg tctttgcgga agaaaaagat ggtattgatg cttctaaatt
      361 agttgatcat caagtagctg ttgcaggact tgcagttatt gccaatccta aagtcaaggt
      421 ttccaatctc agtagtcagc agttgcaaaa gattttttca ggagaatata ccaattggaa
      481 acaagttgga ggagaagatc ttgcgatttc agtgatcaac cgagcagcaa gttctggctc
      541 acgagcaacc tttgacagtg ttatcatgaa aggggtcaac gctaaacaaa gtcaagagca
      601 agactccaat gggatggtta aatcgattgt ttcacaaaca ccaggtgcca tttcttacct
      661 ttcctttgcc tacgttgatt catctgttaa atctttgcaa ttaaatgggt ttaaggcaaa
      721 tgctaagaac gtggctacaa atgattggcc aatctggtcc tacgaacaca tgtataccaa
      781 agataaacca acagggttga ccaaggaatt tcttgattat atgttttcag atgaagtaca
      841 acagaacatt gttacacata tgggatatat ttcgataaat gatatggaag tggtcaaatc
      901 tcatgatgga aaagtaacaa aaaggtaatg gaggaattat ggaaaatcag gaattggcta
      961 aaaaattagc ctcaccatca aagaattcaa gacttgaaac ctttggccgc accattacgt
     1021 ttttgtgtct ggctttgatt gtttttatcg ttgcgatgat tctgatcttc gttgctcaaa
     1081 aagggttgtc aaccttcttt gttgacaagg ttaatctttt tgatttcttg tttggaaagg
     1141 agtggcaacc aagtgtgaaa aatgcggccg gcattcctta tcttggtgct ctgccaatga
     1201 ttacaggatc ctttttggtt accattttat ctgctattat tgccacccca tttgccattg
     1261 gcgcagccgt ttttatgact gaaatctcac ctaaatatgg cgctaaatta ttacagcctg
     1321 cggttgagct tttggttgga attccttcgg ttgtttatgg gtttatcggt ttgcaagtga
     1381 ttgttccttt tatgcgctct atctttggtg gcacaggttt tggaatccta tctggggtct
     1441 gtgtcttgtt tgttatgatt ttaccaacag tgacttttat gacaacagac agtctgcggg
     1501 cggtgcctcg tcattaccgc gaagcgtcta tggctatggg agcaacctgt tggcaaacca
     1561 tttggcgtgt tgttcttaat gcagctcgtc caggtatttt tactgccatt attttcggga
     1621 tggcgagagc ttttggtgaa gccttagcca ttcaaatggt tgtcggtaac tctgctgtaa
     1681 tgccaagttc actaacgaca ccagccgcaa ccttaacgtc tgtcttgaca atgggtattg
     1741 gtaacacggt tatgggaacg gtccaaaata atgtgctttg gtcccttgcc ctagtcttat
     1801 tattaatgag tctggccttc aattcccttg tcaaattaat cacgaaagaa agaaagagaa
     1861 attatgaacg ctaaaaaagt cgataaagta gcaactggta ctctttatac cattgctgga
     1921 attattgtag ctattttagc ttccttaatt ctatatatcc ttgtccgtgg cttgccacac
     1981 atcagctggt cctttttaac aggaaaatcg tcttcgtacg aagctggtgg gggaattggt
     2041 atccagttgt ataattcctt cttcctattg attgttaccc tgattatttc cattccttta
     2101 tcaactggag cggggattta cttggctgaa tatgccaaaa aagggcctgt taccaacttt
     2161 attagaacct gtattgagat tctgtcttcc ctaccatctg tggttgtagg gctctttggt
     2221 tacttgattt tcgttgtgca gtttgaatat ggcttttcta ttatttcagg gtctcttgct
     2281 ttgacggtct ttaatcttcc tcaaatgacc cgtaatgttg aagatagttt actgcatgtt
     2341 catcataccc aaagagaagc aggattagct ttaggcctgt cacgctggga aacagttttt
     2401 tatgtggtta tcccagaagc tctcccgggg atggtaacag gtattgtctt agcttcaggt
     2461 cgtatttttg gtgaagcggc agcgcttatc tatactgctg gtcaatcagc accagctctg
     2521 gattggtcaa attggaatcc acttagtgtt actagtccaa tctctatttt ccgtcaatct
     2581 gagacccttg ctgttcatat ttggaaagtc aatagcgaag ggacaattcc agatgctact
     2641 cttgtatccg caggaagtgc agcagtgcta ttaattttta tccttatttt caacttttca
     2701 gctcgcttta ttgggaagaa acttcattct aaaatgacag cagcgaaata agatattgga
     2761 gaaatcatga cagaatataa ttggaatgaa cgtcatatca tcacttttcc tgaagaaacg
     2821 cttgctctag ctaccaagga tttacatgtt tactatggtg cgaaagaagc tatcaaaggg
     2881 attgatatgc agtttgaaaa acataaaata acagccttga ttggtccatc tggttgtggc
     2941 aaatcaactt atcttcgtag tcttaaccgc atgaacgata ccattgatat tgctcgggtt
     3001 acaggtgaga ttctttatca gggcattgat gttaatcgaa aagacatgaa tgtttacgag
     3061 attcgcaagc acttaggaat ggtttttcaa agacccaatc ctttcgctaa atccatttat
     3121 aaaaacatta cctttgccca tgaaagagct ggtgtgaaag acaaaaaagt tttagatgaa
     3181 attgttgaga cttcactgaa acaagccgcg ctttgggatc aagttaaaga tgaccttcac
     3241 aaatcagcct tcaccttatc agggggtcag caacaacgct tgtgtattgc tagagccatt
     3301 tctgtcaaac ctgatattct attaatggat gaaccagcct ctgcccttga cccgattgca
     3361 acgatgcagc tggaagaaac catgtttgaa ttgaagaaga attacacgat tattattgtg
     3421 acccacaata tgcaacaagc agctcgtgcc agtgattaca ctgccttctt ctatttaggc
     3481 aacctcattg aatatgataa gacacgtaat attttccaaa atgcgcaatg tcagtcaaca
     3541 aacgactacg tttcagggca ctttggttag aaaggtaata tgatgacaga acctatttta
     3601 cagatcagag acctttctgt ttactacaat caaaagaaaa cgttaaaaga tgtctctttg
     3661 gacctttatc caaatgaaat aacagcttta attggtcctt ctggatctgg gaaatcaacg
     3721 ctcttgcgtt ctatcaaccg tatgaatgat ttgaatcctg aagtaaccat tacaggctct
     3781 atcgtctata acggacacaa tatttatagt ccaaggacag atacggttga tctacgcaaa
     3841 gaaattggta tggtattcca acaacccaac ccatttccga tgtctattta tgagaacgtg
     3901 gtttacggcc ttcgcttaaa aggtatcaga gataaatcaa tcttagacca tgctgttgaa
     3961 agctctctta aaggggcttc catttggaat gaagtcaaag atcgtttaca tgattctgcg
     4021 gttggcttat caggcggaca gcaacagaga gtttgcatag ctagagttct tgctaccagc
     4081 cccagaataa tccttttaga tgaacctacc tcagctcttg atcctatctc agctgggaaa
     4141 attgaagaaa cattattgtt attaaaaaaa gattatacac tggctattgt gacccgctca
     4201 atgcagcaag cctcacgttt atcagatcga acaggttttt tcttggaggg tgacttactt
     4261 gagtgtggtc ctacaaaagc tatgtttatg aatcctaagc gcaaagaaac cgaagactat
     4321 atttctggta aatttggttg agaaagacgt atatggttga cccctctgtg tttttattat
     4381 aatgactaga ttgtaggaag aaaaaatcat gttaagaacg aaatttgaag aagaattaga
     4441 taaattgcat aaccagtttt attcaatggg aacggaagta ttggcacaaa tcaataaaac
     4501 tgttcgtgcc tttgttagtc atgatcgtga attggccaaa gaagttatcg aagaagatga
     4561 cactattaat aattttgaga caaagcttga aaagaaatcg ctggaaatta ttgccctaca
     4621 acagccagtt tcgaatgatc ttagaatggt tattactgtt ttaaaggcat caagtgatat
     4681 tgaacgtatg ggtgaccatg ctgcttcgat tgccaaggcc actattcgta tgaaaggtga
     4741 ggagcgtatt ccagtggttg aagaacaaat caaccttatg ggaaaagctg tgaaacagat
     4801 ggtagaagaa gcccttaatg catatatcaa tgctgacgat acaaaagctt atgaaatcgc
     4861 agcttctgat gagattatcg atcaatattt ccgtaatatc caaaccttag ctgttgaaga
     4921 aatccgtaag tctccagatg ctgtttttgc aggaaaagaa tattttcaag tattaatgta
     4981 tttggaacgt attggtgact atgctcgcaa catttgtgaa tggattgttt atcttaaaac
     5041 tggaaaaatt attgaattat aagaaattat gctataatga gactgggaaa cagcttgcca
     5101 gtccctggtt ttatacgaaa ttgtgcaagt tagaaaatga actaacaaga aaagttttct
     5161 ttgctgaatc tgattttgtt aggttaacaa atatatgtag acagagatga tacttaggta
     5221 tcatctgttg atctattatg atttgttatt agaattaagg agttatatga agacagtaga
     5281 acatctcatc gaaacatttg tgcctgaaaa ttataacatc tttttagata ttaatcgtca
     5341 gactaaaaca tttacgggga atgttgcgat taatggtgaa gctttagata atcatgtggc
     5401 tttccaccaa aaagatttgg acattaagtc tattttgctc gataatgaag ctgttattta
     5461 tcaagtggat aacgacaatg aagttgtgcg tgttgaatta cctgaaacag gaatgatgac
     5521 gcttgtcatt gaattttcag gtagcattac agataatatg acaggtattt acccttcata
     5581 ttacactaaa aatggtgaga aaaaagaagt catttcaact cagtttgaaa gccattttgc
     5641 acgtgaagct ttcccatgta ttgatgaacc acaagcaaaa gctacttttg atttaagctt
     5701 gacctttgat caagaaattg gtgagatagc gttatcgaat atgccagaag tcaatattga
     5761 ccgtcgcgag gagactggtc tttggacatt tgatacgaca ccaagaatgt catcttactt
     5821 gctagcgttt gcgctaggag aacttcatgg aaagactgta gaaagcaaaa aaggaacaac
     5881 tgttggtgtg tatgcgacaa cagcgcatcc tcttagctcg cttgattttt cattggatat
     5941 tgcagttcgt gttatcaact tttatgaaga ctattttgga gtacattacc caatcccgca
     6001 atctcttaat attgcgcttc cagatttttc atctggtgct atggaaaatt ggggtttaat
     6061 cacttatcgt gaaatttatt tattagtaga tgaaaattcg actgttcaaa gccgacaaca
     6121 agttgcactt gtaatcgccc acgagatagc acaccaatgg tttggaaact tggttacaat
     6181 gaaatggtgg gacgatctgt ggttaaatga gagttttgcc aacatgatgg aatatgtctc
     6241 tatcgaggct attgagccgt cttggaaaat aatagaagat ttccaaacag gtggtattcc
     6301 tcttgcttta aaacgtgatg ctactgatgg tgttcaatca gtgcatgttg aagtcaatca
     6361 tccagatgag ataaatacat tgtttgatcc tgctattgtc tatgcaaaag gtagtcgttt
     6421 gatgcatatg ttacgccgtt ttattggtga tcgtgatttt gccattggct tacatcacta
     6481 ctttgaaaaa tatcaatatc gaaatactgt cgggcgtgat ttatggaata ttttatcaga
     6541 tacttcaggc aaagacgtag ctgcttttat ggatgcttgg cttgaacaac caggttatcc
     6601 agttttaaca gctcgccttg aaaacgatca attgattttg agtcaaaaac aattttttat
     6661 tggaaaaggt gaagaaaaag gtcgcctttg gccgattcct ttaaacacta attggcatgg
     6721 ccttcctgaa actttgacag aggcagaaat ggtcattcca aactttagtc aattggcagc
     6781 agaaaatgaa ggagctctac gtttcaatat tgataataca gctcattaca ttacagacta
     6841 ccaaggatca ttattagatg ccttagtcac tgaacttgcc caacttgata atacttctgc
     6901 tttacaagtt attcaagaac gtcgtttatt ggcagatagt ggcttgattt cctatgctga
     6961 attggttgac ttaattgctc aattggacga ttctaagtct tatatggtag cagaagcggt
     7021 tcagcaagtg gtctctggtt tgaaacgctt cattgatgaa ggaagcttag cagaaaaatc
     7081 ctttaatcgc ttggtaacca ccatctatca agaagacttt aatcagcatg gttttgagaa
     7141 aaaagctgac gaatcagatg aagatgagat ggtacgtcag gttgccttgg gccgcttgtg
     7201 gctagcagaa aatcagacga ttattgacgg attgagaact atttttgaag catatcaaaa
     7261 caatattgca agtattccag cagctgttcg tcgcttggtt ttagccaacc aaatgaaata
     7321 ttttgaaaca gatagcttgg ttgatattta ttttgagact tatgtagcaa ctactgataa
     7381 taatttacgc agtgatttaa cggttgcttt tagtcaaaca aaacaaccaa ctactattag
     7441 acgtattctt gttagtttaa aagataaaga tattattaaa cctcaggatt tatcttattg
     7501 gtacaatgct ttgcttggac aaagctttac tcaagatatc atttgggaat gggctagaga
     7561 aaattgggat tggattaaat ctgcacttgg tggagatatg agctttgata aatttgttat
     7621 ttatccagca agtaacttta aaacaccaaa acatctagca gaatataaat ctttctttga
     7681 acctaagcta gatgatatgg ctattagccg taatattacc atgggaatta atgaaattga
     7741 agcacgtgtt gctttgatca ctaaagaaaa agaagcagtt attgcagctc tcagtcatta
     7801 ctagaccata agcaaaagag ttgggaggac tttccagctc ttttttgtag tgagctagct
     7861 tcctttctta gttccgtcta taactaaatg attatccata gtttaattca aaaggagatt
     7921 gcactttaaa gcgaaggcta ttagcctctt aatacttttt ttaccaggtg gtgaataaga
     7981 acaattataa atgatttaat cattatgctt taaa
//
LOCUS       DATUYR010000061         7760 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.061, whole genome shotgun sequence.
ACCESSION   DATUYR010000061 DATUYR010000000
VERSION     DATUYR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.061"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            202..2442
                     /locus_tag="VCI33_001403"
     CDS             202..2442
                     /locus_tag="VCI33_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEP2832322.1"
                     /translation="MKQTKVLTGIAVAALSTGTGLVQADDLSNTRPAKAAEVVTHTEV
                     TENQVADAKTKADEATKAVKDQEATVKAIEAEKSQAQQSLVEATTAVNDTEKLASEAT
                     PDGLTKAQEETEASQSAVTDAQAQLEAAQEAESKAQTAVDHQKETVAKASQAVNQAKA
                     ALTTSNQAADVSAAEEKVLEAQEAVTKAETQVSQAQEADAKHESDVRHAKEELDLKSQ
                     KLTEKQTTLEQVMAAIEAERLTKPVENGTYFNQRDNEWQKAYGGKTFAATGCVPSALA
                     MVFSELAKREVTPTEIADYLWHNTDEFNKHYGGTSGKGLVAATNHYGFASTHLDSQST
                     IIAALQAGHHVLAAVQNNKFSPWGSQYSHEIVLRGYSNGNTYVYDPYNRANIGWYPIA
                     NLWNEQSRDAIDTAEVGVPFFKITTQKMAQLEMAKAVANEGLDEAKQAVKQAEANVTA
                     LEQVISQTSQAKEQLAAAKANLVTAKDMLDKAKQTAKLTEQDLETKTANLKQAQEHLT
                     LKQDDLAKAQTELAAQEKSLEQLTQVLSLAQENLGRATKAVEQTKADLTQKQAYVTNL
                     KQVTKLRQESQAKVNQAKAVLSEASARLEQELETLKTLQAKEANLTAQYQAIFKAYQA
                     VLAVKHHSQLAESKAKLEAKGEEAVPVVNEVGHVTDYVSGPTQLADAVAVRTEETSSK
                     TKNTSPVSVKLMAKQGHQKALETIVTSQSSLPQTGEQVVSILSLVGLNFLGLSSFLGL
                     RKQEEK"
     gene            2612..6670
                     /locus_tag="VCI33_001404"
     CDS             2612..6670
                     /locus_tag="VCI33_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP2832323.1"
                     /translation="MKQMETKGYGYFRKTKAYGLVCGITLAGALTLGTTSVSADDVTT
                     LNPATNLTTLQTPPTADQTQLAHQAGQQSGELVSEVSNTEWDNAVTTAQKAGVTVKQS
                     EKVTHDSLSSAQADLEKQTQAVTEATTKQEANTTAINQAVSENKAIDQANRDEKARVD
                     ALNTKGEADTKAKNETGQAQVDAQNKQAQAAADAMNAKLKADYEAKLTEIKQIESENE
                     AIRQRNQQASQATHQANQAAQAAYQEKLAEIERIKAENAAIRDRNAKAQQEAERQNQA
                     LQAAYEAKLAEIKQIESENAAIRQRNEQAGQATNQTNQAAQAAYQEKLAEIERIKAGN
                     AAIRDRNAKAQQEAERQNQALQAAYEAKLAEIKQIESENAAIRKRNEQAGQTTNQTNQ
                     AAQAAYQEKLAEIERINAENAAIRDRNTKARQEAERQNQQLKTTYEAQLSAYQQALQA
                     KKEAENKAIDQVVFGIDAKANGVDNAEYGNSIMTVTTQPDGSFVFKHDMIDGVKTIGH
                     GTLTGKINHHYEANKDGSITAYIDSVTFDKYEYQNVAKNDAVDKNIAFRILSATGQEL
                     FVKAHDGDKTFSETLNKTVSLKLTYQLKPHEAVKDIKVFQLHDDWVHDTHGSALVSYV
                     NNNDAVPHIEVPEKPVEPDMVTPKVEQEKPVPEAPQKPTDGVPNLEKEKPVPPTPVKP
                     EAVKPVLEQEKPVPEAPQKPTDDVPNLEKEKPVPPTPVKPEAVKPVLEQEKPVPEAPQ
                     KPTDDVPNLEKEKPVPPTPVEPEAIKPDLQSFTPEVYDPIKPVVKPHVTVPEKVVYEV
                     MVHPVQVKQTPTNVKSVTNSDQVNIDGQLVPKGSTVTWELVNTSLKAGRQDITSYELT
                     DPLPDGFELDVTATQTLSPEWVITTDEAGKVSLKASQSLLAHFNAKRDQDVEVPKVSL
                     VGRLLNDAGTYHNTFKTVVTTPTGSYTVISNTPVIYTPGNDPKTPRNPGGDNPTPHDN
                     LIQPTKTIVDDKGQSIDGKSVLPNSTLTYVAKQDFDQYKGMTAAKESVMKGFIYVDDY
                     KDEAIDGHSLVVNSIKAANGDDVTNLLEMRHVLSQDTLDDKLKALIKASGISPVGEFY
                     MWVAKDPAAFYKAYVQKGLDITYNLSFKLKQDFKKGDITNQTYQIDFGNGYYGNIVVN
                     HLSELTVHKDVFDKEGGQSINAGTVKVGDEVTYRLEGWVVPTNRGYDLTEYKFVDQLQ
                     HTHDLYQKDKVLATVDITLSDGSVITKGTDLAKYTETVYNKETGHYELAFKQDFLAKV
                     VRSSEFGADAFVVVKRIKAGDVANEYTLYVNGNPVKSNKVTTHTPEQPQPVTPKAPAL
                     PSTGEQGVSILTALGAALLSLLGYVGLKKRQQ"
     gene            6932..7237
                     /locus_tag="VCI33_001405"
     CDS             6932..7237
                     /locus_tag="VCI33_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEP2832324.1"
                     /translation="MEKSTLKLKPDFIGKAWYEKLGNAPRRIDGEAGQFQRHIVSNEK
                     HGVFHVITPALAQVFKTDDVVEIDGEAIFFEDRALNGNSVAPALNVRATGLKLAGGK"
     gene            7240..7695
                     /locus_tag="VCI33_001406"
     CDS             7240..7695
                     /locus_tag="VCI33_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP2832325.1"
                     /translation="MAKVGLNFGEKIQIVDKSYRVLNDGLVDLKEIFGDLSFRSFEET
                     DLIYEDDMTQPRRQDGSYPQIPTGESRGLVVGIFSPVQQQTEFVTLVDMSLAEFEALQ
                     LKYREPVELEGIIVTYSSVGSNQFKLFASKIKRSVSQKVANEGNHKQEK"
BASE COUNT         2723 a         1452 c         1667 g         1918 t
ORIGIN      
        1 tacacatggt cataattatg actttggctg ttttatttat ggtataggaa atgacggaaa
       61 agataagttc cgtgatagat ggtttaaaga aggagtcatt ttctattgaa ttttaaaata
      121 attaaaaaaa agatgaccca aggtaacatt aaagtagtat aataatctta ataaaggaag
      181 ttataggaag ggcaactact tatgaaacaa acaaaagtat taacaggaat cgcagtcgca
      241 gctttatcta caggtacagg tcttgtgcag gcagatgatt tatcaaacac aagaccagct
      301 aaagcagctg aagttgtgac acatacggaa gtgacagaaa atcaagtggc ggacgctaaa
      361 acaaaagctg atgaggcaac gaaagccgtt aaggatcagg aagctacggt taaggctatt
      421 gaggcagaaa aatctcaagc acaacaatct ttagttgagg cgacaacagc agttaatgat
      481 accgaaaagt tagctagtga agcgacacca gatggtctaa caaaagccca agaagagact
      541 gaggctagtc aaagcgctgt tactgatgca caagcacaac ttgaagcagc tcaagaggct
      601 gaatcaaaag cccaaacagc agttgaccat cagaaagaaa cagtcgccaa agcgtctcaa
      661 gctgttaatc aagccaaagc agctctcacg acaagtaatc aagccgctga tgttagtgca
      721 gcagaagaaa aagtgttaga ggctcaagaa gcggttacga aagcagaaac acaagttagt
      781 caggctcaag aagcagatgc taaacatgag tctgatgtga gacatgccaa agaagagctt
      841 gaccttaagt ctcaaaaatt aacagaaaaa caaacaactc tagaacaagt catggctgcg
      901 attgaagcag agcgtctgac taagccagtt gaaaatggca cttactttaa ccaaagagat
      961 aacgagtggc aaaaagccta tggtgggaag acctttgcgg cgacaggatg tgtgcctagt
     1021 gctttggcta tggttttttc agagctggct aaaagagagg tgacaccgac tgagattgct
     1081 gattacttat ggcataacac agatgagttt aataaacatt atggtggtac aagtggtaaa
     1141 ggacttgtag ctgctactaa tcattatggc tttgcttcaa cacatttaga tagtcaatca
     1201 actattattg cagctttaca agcaggtcat catgttttgg ctgcagtaca gaataataaa
     1261 tttagtcctt ggggatcaca atatagtcat gaaattgtct tgcgtggtta ctcaaatggc
     1321 aatacttatg tttacgatcc ttataatcga gctaacattg gttggtaccc tattgctaat
     1381 ttatggaatg aacaaagtcg tgatgctatt gatacagcag aggttggagt acctttcttt
     1441 aaaataacaa ctcaaaaaat ggcacaactc gaaatggcaa aagcagtagc taatgaaggc
     1501 ttagatgaag ctaagcaagc tgttaaacaa gcggaagcaa atgtgacagc cttagaacaa
     1561 gttatttctc aaacctcaca agccaaagaa caattagctg cagctaaggc taacttagtc
     1621 acagccaaag atatgctgga taaggctaaa caaacggcta aattaacaga acaagactta
     1681 gaaacgaaaa cagctaatct taagcaagct caagaacatc taacccttaa acaagatgac
     1741 ctagctaaag ctcaaacgga gctagcagct caagagaaaa gcttggaaca gttaactcaa
     1801 gtgctttcat tagctcaaga aaatcttggc cgtgctacaa aagcagttga acagactaag
     1861 gctgatctta cccaaaaaca ggcttatgtg acgaatctta agcaagtgac aaaactgaga
     1921 caagaaagtc aagccaaagt aaaccaagct aaggcggttc tcagtgaagc aagtgctcgt
     1981 ttagaacaag aacttgagac cttaaaaacc ttacaagcca aagaggctaa cctaacagca
     2041 cagtatcaag ctatctttaa agcctatcaa gctgttttag ctgtgaaaca ccactcacaa
     2101 ttggctgaaa gcaaagctaa gcttgaagct aagggagaag aagctgttcc tgtggtgaat
     2161 gaagtgggac acgtaacgga ttatgtgtct gggccaacac agttggctga tgctgtagca
     2221 gtgagaacag aagaaacatc atcaaaaaca aagaacactt ctcctgttag tgtgaaattg
     2281 atggccaagc aaggtcatca aaaggctctt gaaactatcg ttacttcaca aagtagcctt
     2341 cctcagacag gtgagcaagt tgtctctatt ttgagcttag taggtttgaa ttttttaggc
     2401 ttaagtagtt ttttggggct taggaagcaa gaggaaaagt aataaaatat agaacaagag
     2461 gtcattcttt agagaatggc tttttgattg gagttatgat gaaaatagtt tttaattgga
     2521 aggcaagatt accttccttt tctttttagt aaaacatata agacctggga tcgtcacaaa
     2581 aagcaaccta gaagagagat gagaaaataa catgaaacaa atggaaacta agggttatgg
     2641 ttattttaga aaaacaaagg cctatggtct tgtttgtggt attactttag cgggagcgtt
     2701 gacgttgggt acaacaagtg tttctgcgga tgacgtaact actttaaacc cagcgactaa
     2761 cttaacaacg ctacaaacac ctccaacagc tgatcaaaca cagctagctc atcaagctgg
     2821 ccagcagtca ggggagttgg tatcagaggt gagcaatacc gaatgggata atgctgtcac
     2881 aacagctcaa aaagcaggtg ttactgtcaa acagtctgag aaggtcacac atgatagctt
     2941 atcatctgcg caagccgatt tagagaaaca aactcaagct gtcacagaag cgacaactaa
     3001 acaagaagcc aacacaacag ctattaatca agcagtttcg gaaaataaag cgattgatca
     3061 agctaatcgt gatgaaaaag ctagagttga cgctcttaat actaaagggg aagctgatac
     3121 caaagctaaa aatgaaactg gtcaagccca agtagatgca caaaataaac aagcccaagc
     3181 agcagctgat gccatgaatg ccaaattaaa ggcagactat gaggccaagt taacagaaat
     3241 caagcagatt gaatcagaaa atgaagctat ccgtcagcgc aaccaacaag ctagtcaagc
     3301 gactcatcaa gccaatcaag cagcccaagc tgcctatcaa gaaaagctag cagagattga
     3361 gcgcatcaaa gccgaaaatg ctgccattcg cgatcgtaat gctaaggctc aacaagaagc
     3421 agagcgtcaa aaccaagcgc tccaagcagc ttatgaagca aaattggcag aaatcaagca
     3481 gattgaatca gagaatgctg ctatccgtca gcgtaatgaa caagcaggtc aagcgacgaa
     3541 tcaaaccaat caagcagctc aagctgccta tcaagaaaag ctagcagaga ttgagcgcat
     3601 taaagctgga aatgctgcca ttcgcgatcg taatgctaag gctcaacaag aagcagagcg
     3661 tcaaaaccaa gcgctccaag cagcttatga agcaaaattg gcagaaatca agcagattga
     3721 atcagagaat gctgctattc gtaagcgtaa tgaacaagca ggtcaaacga cgaatcaaac
     3781 gaatcaagcg gctcaagctg cctatcaaga aaagctagca gagattgagc gtattaacgc
     3841 cgagaatgcc gccattcgtg atcgcaacac taaagctcga caagaagcag aacgtcaaaa
     3901 ccaacagtta aaaactactt atgaagctca attatcagcc taccaacaag cactacaagc
     3961 taaaaaagaa gctgaaaaca aagctatcga tcaggttgtc tttggaattg atgcaaaagc
     4021 caacggtgtt gataatgcag aatatggtaa ttcaatcatg actgttacaa cgcaaccaga
     4081 tggttcgttt gtttttaagc atgatatgat tgatggtgtc aaaacaattg gtcatgggac
     4141 tttaacgggt aaaattaatc atcattatga ggctaataaa gatggtagca ttacagctta
     4201 tattgatagt gtaacatttg ataagtatga atatcaaaat gtagctaaaa atgacgcagt
     4261 tgataaaaat attgcttttc gtattttaag tgccacaggt caagaattat ttgttaaagc
     4321 ccatgatggt gataaaactt ttagtgaaac tttaaataag acagtgtctc ttaagttaac
     4381 ttaccaacta aaaccgcatg aagctgtaaa agatatcaaa gtatttcaac tgcatgatga
     4441 ctgggtacac gacacacatg gttctgcttt agtttcttat gtgaacaaca atgatgctgt
     4501 gccacatatt gaagtaccag aaaagccagt ggagcctgat atggttacac caaaagttga
     4561 gcaagaaaaa ccagtcccag aggctcctca aaaaccaaca gatggtgttc caaacttaga
     4621 gaaggaaaag ccggttcctc caaccccagt taaaccagag gcagttaaac ctgttttaga
     4681 acaagaaaaa ccagtaccag aggctcctca aaagccaaca gatgatgttc caaacttaga
     4741 gaaggaaaag ccggtaccac caactccagt taaaccagag gcagttaaac ctgttttaga
     4801 acaagaaaaa ccagtcccag aagctcctca aaagccaaca gatgatgttc caaacttaga
     4861 gaaggaaaaa ccagtaccac caaccccagt tgaacctgaa gcaattaagc cagatctcca
     4921 aagctttacg ccagaggttt atgacccgat taaaccagtt gtgaaacccc atgtcaccgt
     4981 tcctgaaaag gtcgtctatg aggttatggt tcatcctgtt caagtgaaac aaacgccaac
     5041 aaatgttaag tctgttacta atagtgatca ggttaatatc gatggacaac tggtaccaaa
     5101 aggatcaacg gtaacgtggg agcttgttaa tacttctctc aaagctggtc gtcaggacat
     5161 cacaagctat gaactaactg atccactacc agatggtttt gagttagatg ttaccgccac
     5221 acaaaccctt tctcctgaat gggtgataac aacggatgaa gcaggcaagg ttagtcttaa
     5281 ggcttctcaa tcccttttgg ctcacttcaa tgctaaacgt gatcaagatg tagaagtccc
     5341 taaggtatca ctggttggtc gtttactgaa tgatgcgggg acttatcaca atacctttaa
     5401 gacggtggtc actactccaa ctggtagcta cacggtaatc tctaacacac cagtgatcta
     5461 tacaccaggg aacgatccga aaacacctcg taacccagga ggagataatc caacaccgca
     5521 tgataatctg attcagccaa ctaaaaccat tgtggacgac aagggtcagt ccattgatgg
     5581 gaagtctgtc ttgccaaaca gcacgctgac ttacgtggct aagcaagatt ttgatcagta
     5641 caaagggatg acagctgcca aagaaagtgt catgaaaggc tttatctatg tcgatgatta
     5701 caaggatgaa gcgatagatg gtcattcact cgttgtgaat tccattaagg ccgctaacgg
     5761 agatgatgta accaatctct tagagatgcg ccatgtgtta tctcaagata cgcttgacga
     5821 taagctaaaa gcactcatca aggcatcagg catttctcca gtcggtgaat tttacatgtg
     5881 ggtagctaaa gacccagcag ccttttacaa agcttatgtt caaaaagggt tagacattac
     5941 ctacaacttg tcctttaaac ttaaacagga ctttaagaaa ggtgacatta ctaaccaaac
     6001 ctaccaaatt gattttggaa acggatacta cggtaatatc gtggtaaacc atctgtctga
     6061 actaactgtt cataaggatg ttttcgataa agaaggtggt caatcaatca atgctggcac
     6121 tgttaaagtt ggtgatgagg tcacctatcg cttggaaggt tgggttgttc cgactaaccg
     6181 tggctatgac ttgacagaat acaaatttgt ggatcagctt cagcatacgc atgacctcta
     6241 ccagaaagac aaggtccttg cgacggttga tattacttta tcagatggta gtgttatcac
     6301 taaaggtact gacttagcga aatatactga gacagtttac aataaggaaa caggtcacta
     6361 tgaattagcg ttcaagcaag acttcttagc taaggtcgtt cgttctagcg aatttggtgc
     6421 agatgctttt gtggttgtta aacgcatcaa agcaggtgat gtggcaaatg agtatacctt
     6481 gtatgtcaac ggtaatcctg tgaagtctaa taaagtcacc actcatacgc cagaacaacc
     6541 tcaacctgta actcctaaag caccagcttt accatccaca ggagagcaag gagtatctat
     6601 cttaacggcg cttggggccg ctttactctc actcttaggt tatgtagggc ttaaaaagcg
     6661 tcaacagtaa gttattaatg gataagccat caaagtagat tactttgggt ctatccattc
     6721 tctctagcaa gtaactatta gggggatctc ctttcccttc aaaaaactag ccgaaaaagt
     6781 ttactattgg ttacttgtta gggtgagtgg atagatcttg gtttatcact agaatagtga
     6841 ccgaaaagtc aaaaaactaa gtggtaaggc taggaaataa ttgataagac aatcctagcc
     6901 tttttatttt aaaaatgaaa agaggtaaat tatggagaaa tcaacattaa agttaaaacc
     6961 agattttatt ggtaaagcat ggtatgaaaa attaggaaat gctccccgtc ggattgatgg
     7021 cgaagcaggg cagtttcagc gtcatattgt gtcgaatgaa aagcatgggg tttttcatgt
     7081 gattacaccg gctttagccc aagtatttaa aacagatgat gtagttgaaa ttgatggtga
     7141 agctatcttt tttgaagata gagctttgaa tggtaatagt gtagcacctg cacttaatgt
     7201 acgtgcaaca ggattgaaat tagctggagg taaatagtaa tggcaaaagt aggtttaaat
     7261 tttggagaga aaattcagat tgtagataaa agttatcgcg tattaaatga tggtttagtt
     7321 gacttgaaag aaatttttgg tgacttatca tttcgatcct ttgaagaaac agatttaatt
     7381 tatgaagatg atatgactca accaagacgt caagatggtt cttacccaca aattcctaca
     7441 ggcgaatcac gtggacttgt tgttggtatt ttctcaccag tgcagcaaca aacagaattt
     7501 gttactttgg ttgatatgag ccttgcggag tttgaagcgt tgcagttgaa atatcgtgag
     7561 ccagttgaat tagaaggtat tatcgtgact tattcttctg taggaagtaa tcagtttaag
     7621 ttatttgctt ccaaaattaa acgctctgtt agccaaaaag tagctaatga aggtaatcat
     7681 aagcaagaaa aataaataat aagggagagg taagatgcgt ttactgcctg aatatagagg
     7741 gattaaggta aaaccatata
//
LOCUS       DATUYR010000062         7718 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.062, whole genome shotgun sequence.
ACCESSION   DATUYR010000062 DATUYR010000000
VERSION     DATUYR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.062"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            542..931
                     /locus_tag="VCI33_001407"
     CDS             542..931
                     /locus_tag="VCI33_001407"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEP2832326.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1025..2077
                     /locus_tag="VCI33_001408"
     CDS             1025..2077
                     /locus_tag="VCI33_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP2832327.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            2216..3748
                     /locus_tag="VCI33_001409"
     CDS             2216..3748
                     /locus_tag="VCI33_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832328.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVAERVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGIPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNKEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVVHDGKIQGIVLPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            3741..4805
                     /locus_tag="VCI33_001410"
     CDS             3741..4805
                     /locus_tag="VCI33_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832329.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            4807..5763
                     /locus_tag="VCI33_001411"
     CDS             4807..5763
                     /locus_tag="VCI33_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832330.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(5981..7699)
                     /locus_tag="VCI33_001412"
     CDS             complement(5981..7699)
                     /locus_tag="VCI33_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP2832331.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKHGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
BASE COUNT         2306 a         1284 c         1600 g         2528 t
ORIGIN      
        1 taaccttttt taatgtattt taactgaaaa aggctttcat aattttaaaa aaatgttaaa
       61 ataggtgtat aacaactgat taccaagaga ttattatcat tatcttctaa ggtgtcattg
      121 ttatggtgac aatcaaccat acgctactaa agataggtag cgttcgatca caggttgatc
      181 aagtgattga tacagatatt tataatttta caaatatctg tatcaatcca tgttgaatag
      241 tctatgcttc taaggtgtcg acgttaatgt actgacggga ccgttatagt ctttcttttt
      301 ggagaaacgt cggatgccag tgaactagtt gaagttaaag atgttaattc aaacagtgct
      361 gaagagattg atatcgtttt gaacggtcgt ttacttcaaa gcaaagaaat taccagtcta
      421 tcggagatga tataagtgct aaagttagat cacgttaatt atctatcaag tcgggtgcat
      481 atttcgttaa agtatctaca ggggtttcaa tagatgtagc tattatgaaa ggtgatgtta
      541 aatggggact actgatttag taagttgtgc tgtacaagct agtgagtatg cttatgtccc
      601 ttactctcat tttccagtag gagcagcttt aaaaacaaaa gatggaacga tctatactgg
      661 ctgtaatatt gaaaatgtta gttttggtct gacaaattgt ggagagcgaa cagctatatt
      721 taaagccata tctgatggtc ataaagaatt ggttgaaatt gctatttatg gagaaactat
      781 gcaacctgta tctccttgtg gggcttgtcg tcaggtaatg gcagaatttt ttgctccttc
      841 ttctttagtc actttaattg ctaaaaatgg tcaaactgtt gaaatgactg ttggagattt
      901 actcccctat tcttttacag atttaatata atctgcttta atttctgtta tctagccgtc
      961 ttggctttat atttactttc agcaacttat ttgctagaaa tcttttttag gagggttcat
     1021 attcatgaac aagaaattta ttggtcttgg tttagcgtca gtggctgtgc tgagtttagc
     1081 tgcttgtggt aatcgtggtg cttctaaagg tggggcatca ggaaaaactg atttaaaagt
     1141 tgcaatggtt accgatactg gtggtgtaga tgacaaatca ttcaatcaat cagcatggga
     1201 aggcctgcaa tcttggggta aagaaatggg ccttcaaaaa ggaacaggtt tcgattattt
     1261 tcaatctaca agtgaatctg agtatgcaac taatctcgat acagcagttt caggagggta
     1321 tcaactgatt tatggtatcg gctttgcatt gaaagatgct attgctaaag cagctggaga
     1381 taatgaagga gttaagtttg ttattatcga tgatattatc gaaggaaaag ataatgtagc
     1441 cagtgttacc tttgctgacc atgaagctgc ttatcttgca ggaattgcag ctgcaaaaac
     1501 aacaaaaaca aaaacagttg gtttcgtggg cggtatggaa ggaactgtca taactcgatt
     1561 tgaaaaaggt tttgaagcag gagttaagtc tgttgacgat acaatccaag ttaaagttga
     1621 ttatgctgga tcatttggtg acgctgcaaa aggaaaaaca atcgcagcag ctcagtatgc
     1681 agcaggtgct gatgttattt accaggcagc aggaggcact ggagcaggtg tatttaatga
     1741 agcaaaagct attaatgaaa aacgtagtga agctgataaa gtttgggtta ttggtgttga
     1801 ccgtgatcaa aaagacgaag gaaaatacac ttctaaagat ggcaaagaag caaactttgt
     1861 acttgcatca tcaatcaaag aagttggtaa agctgttcag ttaatcaaca agcaagtagc
     1921 agataaaaaa ttccctggag gaaaaacaac tgtctatggt ctaaaagatg gcggtgttga
     1981 aatcgcaact acaaatgttt caaaagaagc tgttaaagct attaaagaag cgaaagcaaa
     2041 aattaaatct ggtgacatta aagttcctga aaaatagtgt ttaagaacaa gaagcgcgac
     2101 ctttgtcggt cgctcttttt aaactgagat aatttttatc tcagtaaagt ttgtgatagg
     2161 tgatagatca taggctttac tgaaataaaa gattgcattg aaaggaaatg atcctatgac
     2221 acagcatgtc attgaaatga gagaaatcac gaaaaaattt ggtgattttg ttgcaaatga
     2281 ccacattaac ttaaatgtca gaaaaggtga gattcatgcc ttattgggtg aaaatggggc
     2341 aggtaaatca actttgatga atatgctagc tggtctctta gagcctacta gtggagaaat
     2401 tgtcattaat gacaagccag ttcaaattga ttcgccatct aaatcagcta agcttggtat
     2461 cggaatggta catcaacatt tcatgttagt agaagccttc acggttgctg agaatattat
     2521 tttaggaaat gaagtagtaa aaaatgggtg cttagatctt aaccaagcat cgaaagatat
     2581 taaggtacta tccgaaaaat atggccttgc aatcaatcca tcagctaaag tttctgatat
     2641 ttcagtagga gcacaacaac gtgttgagat tttgaaaacc ttgtatcgtg gtgcagatat
     2701 tcttatcttt gatgagccaa ctgccgtttt aacacctgca gaaattaaag aattgatgac
     2761 tatcatgaag aatctcgtga aagaaggaaa atcaattatt ttaattaccc ataaactgga
     2821 cgagattcgt gctgtcgccg agcgtgtcac agttattcgt cgtggtaaga gtatcgaaac
     2881 agttgatgtg gcaggagcaa cttcacaaga tttagctgag atgatggtgg gacgctcggt
     2941 ttcctttaca actagcaaaa aagctgctga accaaaagat gtggtcttat ccatcaaaaa
     3001 tcttgaagta gatgaaaata gagggattcc agcagttaag gggctatctc tagatgtccg
     3061 agctggtgaa attgtaggga ttgctggaat tgacggaaat ggtcaatctg agttaattca
     3121 agctattaca ggtttacgta aagtcaaatc tggaagtatt atgattaaaa acaaagaagt
     3181 cactcatttg tcttcacgca aaattactga gttatcagtg ggtcacgtgc cagaagatcg
     3241 tcatcgagat ggtttgatac tagatctttc tttagctgaa aatacagcct tacaaaccta
     3301 ttacaaacaa cctctaagtc aaaatggaat cttaaattat actaaaatta atgactatgc
     3361 tcgtcaatta atgaaagaat ttgatgttcg cggagccaat gagctagtac cagctagagg
     3421 attttctggt ggtaaccaac aaaaggccat tattgctcgt gaagtggacc gtgatccaga
     3481 tttacttatt gttagccaac caacacgtgg tcttgacgta ggagctattg aatatatcca
     3541 taagcgtttg attaaagaac gtgataaagg taaagctgtc ctcgttgtta gttttgaact
     3601 tgatgaaata ttgaatttat ctgatcgaat tgctgttgtt catgatggta aaattcaagg
     3661 tatcgtattg ccagaaaata ccaataaaca agaattgggt attttaatgg caggtggttc
     3721 aattcataag gaggaaggtc atgtctaaaa atgcgcaaaa aattgctgta ccattgattt
     3781 ctgtcttgct aggattttta cttggcgcta ttattatggt tatctttggt tatgatccta
     3841 tctgggggta tgaaggttta ttccaaattg cctttggtag tgttaaaaat attggagaaa
     3901 tattccgttc aatggggcca ttgattttaa tagcgcttgg ctttactgtt gctagccgtg
     3961 caggcttctt taacgttggt ttgtctggac aggctttagc tggatggatt tcagcaggtt
     4021 ggtttgcact cttaaatcca gatatgcctc gtccattatt gattcttatg acagctctta
     4081 tcggaatgat tgctggtggt attgctggag ctattccagg gatacttcgt gcttatcttg
     4141 gtacaagtga agttatcgtt acgattatga tgaactacat cattttatat gtcggaaatg
     4201 ctattgttca acgtggttat cctgagagtg tcaaacaaag tattgattca actattcaag
     4261 ttagtgataa tgctagttat caaacgcatt ggttatcagc cctaactaat aactctcgta
     4321 ttaatattgg tattttcttt gccattattg ccattgccct aatctggttc ttgcttaaca
     4381 aaactacttt aggttttgag attcgttcag ttggtctcaa cccacatgct tcagaatacg
     4441 cagggatgtc atcaaaacgt actatcattt tatcaatgat tatctctgga gctcttgcag
     4501 gtcttggagg agtagttgaa ggtctaggaa cctttgaaaa tgtttttgta caaggatctt
     4561 ctttagcagt tggttttgac ggtatggcag ttagcttatt agcagcaaat tcaccgcttg
     4621 gaatattttt ctcatcattc ctatttggtg tcttaaatat tggtgctcca ggaatgaata
     4681 ttgcaggcat tccaccagaa cttgttaaag tagtaactgc atcaatcatt ttctttgttg
     4741 gttcacatta cctcattgaa cgttacatta ttcgccctaa aaaattagtg aaaggtggta
     4801 aataagatga gtttagtaac aatttttgcc ttattggtgt catctatgct tatttatgca
     4861 acaccactca ttttcacaag tattggagga actttctcag agcgctcagg cgttgttaat
     4921 gttggtttag aaggtatcat ggtcatggga gcgttctctg ggattgtttt caacttagag
     4981 tttgctgaaa catttggaaa agcaacgcct tggatagccg ttttagtcgg tggtattgtt
     5041 ggtttgattt tttctttgat tcacgctgta gcaaccatca actttcgagc tgaccacatt
     5101 gtcagtggta cagtgttgaa cttgttagca ccttcttttg ctgtcttctt ggttaaagct
     5161 atgtacggta agggacaaac agacaacatt caacaatctt ttgggaagtt tgatttccca
     5221 ggtttatcac aaattcctgt gattggtgat attttcttta aaaatactag ccttattggt
     5281 tacttcgcca ttgctttttc gttctttgct tggtttttgc tttataaaac caggtttggt
     5341 ctgcgtttac gatcagttgg ggaacaccct caggcagctg acacacttgg tattaacgtc
     5401 tatttgatga aatactatgg tgtgatgatc tcaggttttc ttggtggaat aggtggagct
     5461 gtttatgctc agtcaatctc cgttaacttt gctgtaacaa ctatcttagg tcctggattt
     5521 attgctttgg cagctatgat ctttggtaaa tggaatccag ttggcgccat gctatctagt
     5581 cttttctttg gcttatcgca aagtttagcc gttattggtg ctcaattacc attactagaa
     5641 aagattccaa cggtttactt acagattgca ccttatatgg taacgattat tattttagct
     5701 gctttctttg gtcaagcagt tgcaccaaaa gctgatggaa tcaactatat taaatctaaa
     5761 taacgttata ttagtagtaa gcctcttagt gaggtttaac agcactccta aagcaagata
     5821 gttaggtcta gctttaaggg tgcttttttg ggacttaccg caagcgtttg gattgccagt
     5881 caccatcaca atagaagcaa gaatagacta aaaagaacta agtcagcggc ttagttcttt
     5941 ttgcttgtct ttcgttttta ctgatagctc ttaagttgtt ttagccaaca aaggtattga
     6001 tttctgtttc aatgttagca attttttctt gagcgatgtc aagagtatca cctattgtgg
     6061 caatatagaa cttgattttt ggttcagttc ctgaagggcg aactgcaatc catgaatcat
     6121 cagctaacgt gtacttcaaa acattacttg gaggagttgt taagttagat attgttccat
     6181 catttttagt agcggtttgt ttttggaaat cttctaataa tacaatatca gtattgttaa
     6241 attgttttgg accattttca cggaatttgt ccataatctt cttaatttca gcagctccat
     6301 ccacacctga aagtgttact gaaatcgttt tttcagcaaa atagccatat tctttgtaaa
     6361 tttcatcaat accatctgca agtgttaaac cacgcgaacg gtaataagct gcgatctcag
     6421 caaccaaaag aactgcttga atagcatctt tatcacggac aaatggttta ataagatatc
     6481 cgaagctttc ttcaaaacca aacatatagg tgtggttatg tttttcttca aactcttgaa
     6541 ttttctcagc gatgaatttg aatccagtta aaacgttaaa cattgtagcg ccataacttt
     6601 cggcaatttt tgttactaat tctgttgata cgattgattt agcgagagca gcattttctg
     6661 gaagtgttcc tgctgtttta tgagcttcca ggatgtattt cgcaataatc gctccaattt
     6721 gatttccaga aaggttttta taagaaccat ctggttggcg aatttcaacg cctaggcggt
     6781 ctgcatcagg atcagtagct actaagacat ctgcatcaac ttctcgtccg agttcttcag
     6841 caagagcaaa ggcagcttgg ctttcagggt taggcgattt aacagtagaa aagtctggat
     6901 ctgccttagc ttgtgattcg actacaacaa cagattcaaa accggcttga gcaagggcac
     6961 ggcgtgtcaa catctcacca gttccgtgga gaggagtata gacaattttc atgtcttttc
     7021 caaagttatt gattaaatct tgattgatat taacatcttt aacctctcta agatattcca
     7081 tatcaacatc ttcaccgata acttgaataa gacctgatga tttgctttca tctaaatcag
     7141 caagttcaac tgcaaatgga ttttcaatag cacggataaa gtctgttagt gcatcagcat
     7201 cagctggtgg tagttgacct ccgtcttgtc catagacttt ataaccattg aaaggtgctg
     7261 gattgtggct tgcagtaacc ataataccag cataggcatt taaatggcga acagcaaaag
     7321 agagttctgg tgttggacga agagcttcaa atacgtaaga tttaatccca tgctgtgcca
     7381 agacctgtgc agactcaaaa gcaaattctg gagagaagtg gcgcgaatca taagcgatgg
     7441 ctacaccatg ttttttagct tcctctccct tagtttcgat taattttgct aatccttcgg
     7501 ttgcttgacg gacaacataa atattaatac gatttgtacc agcaccaata taaccacgca
     7561 taccagcagt accaaattct aagtttgtgt aaaaagcatc ttcttttgtt ttttcatcca
     7621 ttgatagtaa ttcttgacga agatagtcag gaagctgctc aaaatctaac catttttgaa
     7681 aattttctgt gtaagtcatt gaaatactcc ttcaatat
//
LOCUS       DATUYR010000063         7440 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.063, whole genome shotgun sequence.
ACCESSION   DATUYR010000063 DATUYR010000000
VERSION     DATUYR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.063"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..349
                     /locus_tag="VCI33_001413"
     CDS             <1..349
                     /locus_tag="VCI33_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEP2832332.1"
                     /translation="FPLYVCFFFFVYISNASKITINIVEKMGIIFVISAIIGATVLAM
                     YLTWTPVGSSTVLGVQSRYLIGIIPLVLLLFSSQQQKFKQIEDILSDKLAIHVSLLFI
                     LAMLMSTIFRYYH"
     gene            443..1666
                     /gene="pepT"
                     /locus_tag="VCI33_001414"
     CDS             443..1666
                     /gene="pepT"
                     /locus_tag="VCI33_001414"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEP2832333.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALELAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            1707..2195
                     /locus_tag="VCI33_001415"
     CDS             1707..2195
                     /locus_tag="VCI33_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEP2832334.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHFEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(2182..2379)
                     /locus_tag="VCI33_001416"
     CDS             complement(2182..2379)
                     /locus_tag="VCI33_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEP2832335.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            2428..2904
                     /locus_tag="VCI33_001417"
     CDS             2428..2904
                     /locus_tag="VCI33_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP2832336.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKASSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            2919..3599
                     /locus_tag="VCI33_001418"
     CDS             2919..3599
                     /locus_tag="VCI33_001418"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEP2832337.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPISFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWV
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    3623..3746
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3761..4291
                     /gene="infC"
                     /locus_tag="VCI33_001419"
     CDS             3761..4291
                     /gene="infC"
                     /locus_tag="VCI33_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEP2832338.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            4333..4530
                     /gene="rpmI"
                     /locus_tag="VCI33_001420"
     CDS             4333..4530
                     /gene="rpmI"
                     /locus_tag="VCI33_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEP2832339.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            4589..4948
                     /gene="rplT"
                     /locus_tag="VCI33_001421"
     CDS             4589..4948
                     /gene="rplT"
                     /locus_tag="VCI33_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEP2832340.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(5239..7410)
                     /locus_tag="VCI33_001422"
     CDS             complement(5239..7410)
                     /locus_tag="VCI33_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEP2832341.1"
                     /translation="MKKFKTLITGFINTRLGFIITLLFCYWIKTLWAYHTDFSLDLGN
                     IYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNILLISNSIYYREFSD
                     FITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSITKKIKNDQRPFNKR
                     AAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFTLYSGNQTYQAQ
                     KERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLESFQQFLIDYKLKEG
                     DKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSLFGLNSGSFMVNYG
                     GENTQFATPSILTQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYFFDSSYFSKQNSKN
                     SFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGESSEEGFPLAKTDD
                     ETINGYFATANYLDAALKSFFDYLKATGLYENSIFVLYGDHYGISNSRNSSLAPLLGK
                     DSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLLHILGIDTSQFVQL
                     GQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNPDEMTIAKTKEIRS
                     AVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKDISAKLGSESTSLY
                     SKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNPKNKE"
BASE COUNT         2418 a         1216 c         1476 g         2330 t
ORIGIN      
        1 tttcccgctt tatgtgtgtt tcttcttttt tgtttatatt agtaatgcta gtaaaataac
       61 tattaatatt gtagaaaaaa tgggtataat ctttgttatt tcagcgatta taggtgcaac
      121 tgtattagct atgtacctga cttggacacc agtagggtcc tcaactgtct taggtgttca
      181 aagtcgttat ctgataggaa ttatcccatt agtgttgctt ctttttagta gtcaacagca
      241 aaagttcaaa caaatagagg atatcctttc tgacaaactg gctattcatg tttcgctttt
      301 gtttattctc gcaatgttaa tgagtactat ttttagatat tatcattaaa ctcttaatta
      361 gaggctttaa aaaacttagc ttgaataagt attttaaagc cttttatgct ataattaaat
      421 tgagaaatta ttgaggtggc ctatgaagta cgataacttg ttagatcgat ttataaaata
      481 tgtcaaagtg aatacaagaa gcgatccaga cagtgagact actcctagta cggagagtca
      541 agaagcattt gccttaacga ttttaaagcc tgaaatggag gcaattggtt tgcaagatgt
      601 tcattataat ccagttaatg gctatcttat tggaactctg cctgccaata atccaacctt
      661 gacgcgtaaa attggtttta tagcacatat ggatacggct gatttcaatg ctgaaaatgt
      721 taatccgcag attattgaca attatcaagg aggggacatt acacttggca gttctaatta
      781 taagttggat ccaaaagcat ttccaaatct taataattat atcggtcaaa cattgattac
      841 cactgatggg actacacttt taggggcaga tgataaatct ggtattgctg aaatcatgac
      901 agcaatagaa ttcttaacat cacaaccaca aatagagcac tgtgacatta aagtggcttt
      961 tggccctgat gaagagattg gggtcggagc tgataagttt gaggtggctg attttgaggt
     1021 ggattttgct tatactatgg atggcggtcc tttaggcgaa ttgcaatatg aaactttcag
     1081 cgctgcagct ctagaagtga cttttttagg cagaaatgtt catcctggta cggctaagga
     1141 tcaaatgatt aatgcccttg agttagcaat agattttcat gagaagctac ctgctaaaga
     1201 gcgacctgaa tacacagatg gttatcaagg cttctatcac ttaaccggac taactggaac
     1261 agttgaagag gcacgcgcat cttacattat tcgtgatttt gaagaagcat catttgaagc
     1321 tagaaaagtg aaagttgaaa atatagctca atcgatgaat gctcagctag gaacaaaacg
     1381 tgtcttggta gagcttaatg accaatacta caatatgaaa aaagttatcg aaaaagatat
     1441 gactgctatt gagttagcta aagaagtaat ggaagagctt gctatcaagc ctgtaatcga
     1501 acccatacgt ggaggaacag atggctctaa aatttcattt atgggtattc ccactcctaa
     1561 tatttttgca gggggtgaga atatgcacgg gcgttttgag tttgttagct tacaaacgat
     1621 ggagagagct gtcgatgtca ttattggact tgtttgcaaa gcctaattat catgtaattg
     1681 atatttagtg gaaggggctt ataaaaatga ttaaactatt tggtaaaata aggtatcact
     1741 ggcagccaga gttgtcttgg tctattattt attggtctat tgcgtttgca cctatttttg
     1801 taggtttatc tttgttgtat gagcgcactg aaatacctag tcgggttttt attctatttg
     1861 ctatttttgc tgtcctagtt ggcattggat tacatcgtta tttcattatt gaaaataatg
     1921 gtattttaag aatcgtatct tttaaattgt ttggtccacg taagctactt atttctacta
     1981 ttactaagat tgaggtgacc aaatcaacac tttgtctgca ctttgaagat aaaagttatc
     2041 tcttttatat gcgcaaatgg cctaaaaaat attttcttga tgccttagcg gttaatccat
     2101 acttccaagg agaagttata ttaagtgata actttattaa attagactat tttgaagttt
     2161 atcagcatga taaaaaagcc cttactcgag ggtaagagct ttagttgggc aagatttgac
     2221 agctaaaata gcgtccttat ctgaaggaca aattgactga cttgtttcag atgagcttga
     2281 aaatgtgacg ataccattgt catgataatc aaaaagtgat gaataggttt ggcatagacc
     2341 acaagcgatg catttttctg gaatgataga tactttcata actattattc taatatgatt
     2401 ttgttaaatt aacaaggggg gacgatcatg gctaaagaac catgggaaga aaaaattgtt
     2461 gatgatacta tagggacacg aacacgtaaa tcaagaaatg ctttcattag cacgccttgg
     2521 ttgactgctt tattaagtgt attctttgtc atcattgttg ctatactttt tattttcttt
     2581 tatacatcaa atagcggtag taatagacaa gctgaaacaa atggttttta tggagcatcc
     2641 actcataaaa aaacaaggaa agcttctaac gctaaaaaag catcaagtag ttcaacaact
     2701 acagacacaa caccttctag cgaagaaaca cttgcttcta gtgaaggaac tggcgaaacc
     2761 cttactgtat tggcaggtga gggggcagct tctattgcag cccgtgcagg tatttctgtg
     2821 gagcagttac aagcacttaa tccagagcac atgactcaag gatattggta tgccaatcca
     2881 ggagatcaag tcactattaa ataattgaag aggaaaaaat gaaagcaatt aaaattgcga
     2941 ttgatgggcc agcttcaagt ggaaaaagta cagtagccaa gattattgca aaaaatcttg
     3001 gctatactta tctagatact ggtgctatgt atcgctcagc aacttatatc gcactgacac
     3061 atggctatac gggtaaagaa gtagccctta ttttagagga acttgagaaa aatcctattt
     3121 cctttaaaaa agcaaaagat ggctcccaat tagtttttct tggggatgag gacgtgacct
     3181 tagccattag gcaaaatgat gtgactaaca atgtttcttg ggtctcagct ttgccagaaa
     3241 ttagagaaga gctggttcat caacaaagac gcattgcaca agctggtggc attatcatgg
     3301 atggtcgtga cattggaacg gttgttctcc cagatgcgga actaaaaatc tttttagtgg
     3361 cttctgtcga agagcgggca gagcgccgct ataaagaaaa cctagaaaaa ggaattgaat
     3421 cagattttga gacgctaaaa gaagagattg cagctcgtga ttacaaggat agtcatcgta
     3481 aggtttcacc tctaaaagca gctgaagatg cccttatttt tgataccaca ggtgtttcta
     3541 ttgatggcgt ggtgcaattt atccaagaaa aagcagaaaa aatagttgac atgtcctaga
     3601 gcctatgtta gaatattaac aatgagaaaa gtagaagtga gagcttctcg ccttgcgact
     3661 taggttgtct ggccctaaca tgtcaaattc ctatggaata ttatgtgtgc gggcttgatt
     3721 tatcaagtcc gctttcgttt ttctctaaaa aaataaagag gtgaagatca tagctaaaaa
     3781 ggatctattc attaatgatg aaattcgcgt tcgcgaagtt cgtctcgttg gtcttgaagg
     3841 tgaacaatta ggtattaaac cattgtctga agcgcaatca ttggctgatg cttcaaatgt
     3901 tgatttggtt ttaatccagc cacaagctgt tcctcctgtt gccaaactta tggactatgg
     3961 aaagttcaaa tttgagtatc aaaagaaaca gaaagaacaa cgtaaaaaac aaagcgttgt
     4021 gactgtgaaa gaagttcgtc tcagtccagt tatcgataaa ggtgactttg aaacaaaact
     4081 tcgtaacggt cgtaaattcc ttgaaaaagg aaataaagtt aaggtttcta ttcgtttcaa
     4141 aggacgtatg attactcata aagagattgg tgccaaggta ctagctgact ttgctgaagc
     4201 gactcaagat attgctatca ttgagcaacg agcaaaaatg gatggacgcc aaatgtttat
     4261 gcaacttgca ccaatttcag acaagaagta attgtcacag tttcattata tctttaagag
     4321 gagaatacta aaatgccaaa acaaaaaaca caccgcgcat cagctaaacg ttttaaacgt
     4381 acaggttcag gcggtttgaa acgcttccgc gcctttacat cacaccgttt ccacggaaaa
     4441 actaaaaaac aacgtcgtca tcttcgtaaa gctggtttgg ttagctcagg agatttcaaa
     4501 cgtatcaaag caatggttac tggtctttaa ttttaaaggc agtagacatt tacgagtatt
     4561 ataagaatta tttgaaggag aactattaat ggcacgtgtt aaaggtggcg ttgtttcacg
     4621 caaacgtcgt aaacgtattt taaaattagc taaaggttac tatggagcaa aacacatctt
     4681 gttccgtact gcaaaagagc aagtgatgaa ctcttactac tatgcatacc gtgatcgccg
     4741 tcagaaaaaa cgtgatttcc gtaaactatg gatcactcgt atcaatgcgg ctgctcgtat
     4801 gaacggtttg tcatactcac aattgatgca cggtttgaaa cttgctgaaa tcgaagtaaa
     4861 ccgtaaaatg cttgctgatt tagcagtagc tgatgcagca gctttcacag ctcttgctga
     4921 tgcagccaaa gcaaaacttg gcaaataaga aacgctatac ctaaaaaata actttctatt
     4981 acctatagaa agttataaag aaacaaaaat gaaggagacg atggacgtct tctttttatt
     5041 atactcaata taataaaaag cagtttccca tgttttatta ccagtaatgt gggatattta
     5101 gatggtagca aggagtttta tagctgattt gttttcttta ggtctaatta gcatattttg
     5161 attactgata aacttgaata tcgccaataa aaaagaagca aaattattat tttgcttcta
     5221 gtcagatcat taaattgatt attctttatt tttggggtta tcctctttta gttcagaagt
     5281 tgccgcgtca gcttctacgg gatttagttc taagtaagat ggtgctttaa aaagtttctg
     5341 agttgattta tgaccatttt tactgtataa acttgttgac tctgatccga gttttgctga
     5401 aatatctttc agttgtttta actgcttagt gtagataaac tggttaggat caatagcttt
     5461 tagaccattt tgagtatcaa agcgcaggag atcacccgtt tgaatagcgt cgctagctgc
     5521 tagttgttga gcaacagcag agcgaatttc cttagttttg gctatagtca tttcatcggg
     5581 attagtgatt tctaaacctg tttgcgtgtt gtacagacgt ccactatagt tagtgtattc
     5641 aggagtcata taagtcccag atgttcgttg tgccacaatc tggctatttt gaggtgataa
     5701 taagtcttgt cctaattgaa caaactgact agtgtcaata ccaagtatgt ggagtaaagt
     5761 aggaagagca tcgatttcac cgccaaaggt ttctttgata ctaccattcg tataacctgg
     5821 aatatggatc atatacggga ctcgttgtag catagcatta tcatattcag accaagtttc
     5881 agaatcttta ccaagaagtg gagcaagact agaattacga gaatttgaaa taccataatg
     5941 atcaccatat aaaacaaaaa tagaattctc gtacaaacca gtggctttca agtaatcaaa
     6001 aaaagattta agggcagcgt ctaaataatt ggcagtagca aagtaaccat tgatcgtttc
     6061 atcgtctgtt ttggctaaag gaaaaccttc ttcactactt tcgcctttta gactagtata
     6121 aggatagtga ttactcacag tgataaactt ggtataaaaa ggttgttgca tctgttcaag
     6181 atattttatg gaatccttaa acatgtactt atcatttaac ccatattgaa aagagttctt
     6241 actattttgt ttagagaagt agctagaatc aaaaaaataa ttatagcccc attgtttata
     6301 agcattattg cgattccaga aagttccaac gttaccatga aagacagcgc tggtatagcc
     6361 acctttttgg gttaaaatac ttggagtagc aaattgtgta ttttcaccac cgtagttcac
     6421 cataaaagaa ccactattta aaccaaataa ggaattctcc atcatggttt cagcatcaga
     6481 agttttacct gctttaacct gatgaaaaaa gttagggaat gctaaggtag catttgagtg
     6541 ataaagcgag ttgataaaag gtgttacctc atattcttta tctccttctt ttagtttata
     6601 atctattaag aattgttgaa agctttctaa atgaagaaca ataatatttt tgccttttcc
     6661 aattccaaaa tactgaggat caggtgctgc ataatgtcct ttgacataag ttttaacatc
     6721 aattaattct tctgcagtag ctccatttcg ttctttttga gcttgatagg tttgattgcc
     6781 gctatagagg gtaaacgctg gtagtcctaa agctctcaca atataagtat ttgaaaagcc
     6841 acgtgttaat agttccggac gatcaatttc tgctaaaaaa agattaatag aaagcaatag
     6901 actagataaa gcggtgatgg caaatgctgc gcgcttgtta aaaggacgtt ggtcattttt
     6961 gattttttta gtgatagata aactaattag aataataaaa tcaagaatat agataatatc
     7021 ccagatgcgt aaaaggttta aagcagagtc ccctaatcca gcggagactt tactactagc
     7081 tagcatggca ctcactgtaa taaagtctga aaattctcgg taatatatag aatttgatat
     7141 caagagaatg ttaaggataa tataaaccac ccaagaacag atataaaagg ctcgggtatt
     7201 cttaacgtaa agagcaaccc ccaaaagtaa aaaagctagt gggattggat tgataatagt
     7261 tagaaagact tggtatatat ttcccaaatc tagtgaaaag tctgtatgat atgcccacag
     7321 ggtctttatc cagtaacaaa agagtagagt aattataaaa cccaatcttg tgttgataaa
     7381 acctgtgata agtgttttaa atttcttcac agtagcaact tccttatcgt tttttcctaa
//
LOCUS       DATUYR010000064         7343 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.064, whole genome shotgun sequence.
ACCESSION   DATUYR010000064 DATUYR010000000
VERSION     DATUYR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.064"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(56..565)
                     /locus_tag="VCI33_001423"
     CDS             complement(56..565)
                     /locus_tag="VCI33_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832342.1"
                     /translation="MELFNTIIGVIALIVAVIALVHSIYYNMVKIKLSDCYISRVDKG
                     YDWMYDFSISNLSNVSVIIKKIELYNKDGKLISDNGFNPFQKYEADMQNEADDYYGLS
                     MPNYYMPLDYQWESSPFKSDTEVYPSSRENFSYYLDEKPVKIKITTDKRIHQFRKYQL
                     FFPHFDNNS"
     gene            complement(824..1033)
                     /locus_tag="VCI33_001424"
     CDS             complement(824..1033)
                     /locus_tag="VCI33_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832343.1"
                     /translation="MNLEELTPLLNSIDDFETVILHSLVGDFVIDHWIEPNRKNETLI
                     FMHNDQTTELKLSAILGTSTIPKSL"
     gene            complement(1183..1422)
                     /locus_tag="VCI33_001425"
     CDS             complement(1183..1422)
                     /locus_tag="VCI33_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832344.1"
                     /translation="MDTKEFMKVVSKHINQNFNVDNQLVEFVVAELNQMNAPITQKQA
                     QHIVNILEYVSKSTSKSTIAAMTNALLELGVLKGD"
     gene            1589..1774
                     /locus_tag="VCI33_001426"
     CDS             1589..1774
                     /locus_tag="VCI33_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2832345.1"
                     /translation="MWGKIEALLIEKKMTKYELSQKAGLNQNCLIDLKKGRKKSLKFD
                     DVVKIADVLGVSLDEFR"
     gene            1853..2149
                     /locus_tag="VCI33_001427"
     CDS             1853..2149
                     /locus_tag="VCI33_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832346.1"
                     /translation="MAIEIFGPEFRKKLLEDLIALNMEAIKIAQTKNAKSIEWITMKR
                     LEKETGWGRTKLTQWREQGKFNFKRSSENGKVLYDLADVNRFLRTSGYEKGETT"
     gene            2146..2283
                     /locus_tag="VCI33_001428"
     CDS             2146..2283
                     /locus_tag="VCI33_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832347.1"
                     /translation="MKLLDFIFAKPKKQEPIKHDTLRASSEKQWAEFDAYMRKQYGRQ
                     A"
     gene            2364..2693
                     /locus_tag="VCI33_001429"
     CDS             2364..2693
                     /locus_tag="VCI33_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832348.1"
                     /translation="MKTKIEIMRDFFKENPESTQRQASEKLGFTENTVKQYIWRDAKR
                     GYCIKDEEGRVTYLNIEDELSLINEWKSEIRRELIEQLLSANRHETSSEQIRMNAKTI
                     NQILGEI"
     gene            2693..2887
                     /locus_tag="VCI33_001430"
     CDS             2693..2887
                     /locus_tag="VCI33_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832349.1"
                     /translation="MEGMKKYINGQIKLIDTIEECVKEKWPEFDDKKVHKISLAIYQG
                     WSLTDINSTLEAIEMDLDRK"
     gene            2884..3168
                     /locus_tag="VCI33_001431"
     CDS             2884..3168
                     /locus_tag="VCI33_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832350.1"
                     /translation="MRIYVNKKGKPSVEFEFEDRRGGMFDTRLMLKESPLKEEFKADM
                     YKAIDDVLKKYEDIFDTPIFKELIIGKEEDIRKMIDYDDKFTELFGGIRH"
     gene            3165..3848
                     /locus_tag="VCI33_001432"
     CDS             3165..3848
                     /locus_tag="VCI33_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP2832351.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLTNYLKGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPTNIFVTAWESTQDLTLESGQIITQ
                     YVPDIRKQVLSQTLGLTDVVGRIQVNPKTGGHGILLQGSDGLYAKNRLDNRTVCKAEE
                     LFNFEGSDA"
     gene            3884..5287
                     /locus_tag="VCI33_001433"
     CDS             3884..5287
                     /locus_tag="VCI33_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2832352.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGHVLFL
                     VHRKELIDQITNSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QIIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKIMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWNIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECSVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QLANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            5292..5774
                     /locus_tag="VCI33_001434"
     CDS             5292..5774
                     /locus_tag="VCI33_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEP2832353.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNERNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQHYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPNSYHVWKEKATGFTQAEIEEDDLPF
                     "
     gene            5792..>7343
                     /locus_tag="VCI33_001435"
     CDS             5792..>7343
                     /locus_tag="VCI33_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEP2832354.1"
                     /translation="MTSNEFIEALSKLTTETDWGDPIFGESVLKAELRKHLFKIVPID
                     HNGYIHKLFYSEMVKDEDVMYFVSDGRKTYRFLFGDTILKTDKQGNEYLTYSVENNFP
                     PFAKLVIDYILGAYTFFENKLYDIRYKQFKLIDDFTLQTKYGFKDSGHILEILQGIHK
                     TLNIQPINYIEPYQIACKDFIIDLENSEIINQPPLQNVSYFKYYEVDYKTAINSKSIA
                     EEYLEYVIADSNSLNNAILQSYFIAQVACGVRPKTNFFISKSGVRTGKGLRHIALSGL
                     FNKIDVELDTLKSNGFEALQAWAMFSGGEMALATEQGDIQGNAMERVLKIIATEKTHV
                     ARAIGQNQSMVNLTSVLCIDTNRTVALSDEMNGRKVLIQFKDRPKTETDYERESIFRK
                     YWLAFTDCDKNPKIDGCIGFLLNSLERFQKIGKWYQWKDVEVFNDIDLDEFQVALINA
                     LQEVDFVQRTDNKEVIDLSLQVYGKSNHALSKAISEIGVRSRSKKVNGKTVRGYEIEN
                     KTRFDKYIL"
BASE COUNT         2746 a         1148 c         1402 g         2047 t
ORIGIN      
        1 aaaaatgctc ctaacggcag cacttatctt aattttatta acgaatatcg ctatcttaac
       61 tattattgtc aaaatgggga aagaataact gatattttcg gaattgatga atacgcttat
      121 cagttgtgat tttgatttta actggctttt catctaaata gtaagagaaa ttttctcgac
      181 tagatggata tacttcagta tctgacttaa atggcgatga ctcccattgg taatctaatg
      241 gcatataata attcggcatt gacaatccat aataatcatc agcttcattc tgcatgtcag
      301 cttcgtattt ttgaaaggga ttgaagccat tatcgcttat tagttttcca tctttgttgt
      361 aaagttcaat ttttttaata atgactgaaa cattcgataa gttactgata ctaaaatcat
      421 acatccaatc gtaaccttta tctactcttg aaatgtaaca atcagataat tttatcttaa
      481 ccatgttgta gtagatagag tgaacaagag caatcacagc aacaattagc gcaatgacgc
      541 cgataattgt attaaataat tccattttta ttcttccaat cgtttttatt ttaattatac
      601 caaaatagaa aggacacata tgaacgaaat agaaaacaaa ctagaagaac ttgaagaaat
      661 ggtaataaac atggatgaag tagatgtagt gattccatgg aagatagcaa aaaacctgct
      721 acaaagagca ggttacttat cagaagagga acatcgccta ctaagttgga gactcggaaa
      781 atcaaaatat gcaggcaaac gttccgaaaa agtccgcaat ttgctagagg gacttaggaa
      841 tagtggaagt tccgagaata gctgataatt ttaattctgt tgtttggtca ttgtgcatga
      901 aaatgagagt ttcatttttg cgattaggct caatccaatg atcaataaca aagtctccga
      961 caagactatg taaaataaca gtttcaaaat catcaatact attcaataga ggagttaatt
     1021 cttctaaatt catttagtta ccctccttcc taataggata actacattat acaacagaaa
     1081 ggaagcacat gagaccaaaa cgatatccgt atagcggaga gaaaaagaga gcctatcata
     1141 agatagaccc tgaactgata aaaaccggta aagttgattg catcaatctc ccttaagtac
     1201 gccaagctct aacaatgcat ttgtcatagc tgcgatagta gatttagagg ttgacttaga
     1261 gacatactcc aaaatattaa cgatatgctg agcttgcttt tgtgtaatag gtgcattcat
     1321 ttgattaagt tccgcaacga caaattcaac taattggttg tcaacattga aattttggtt
     1381 gatatgtttt gagacaactt tcataaattc ttttgtatcc ataattaatc tcctttcttg
     1441 cttatttgac taaacaagat tagaagagta gaggtctctc gagagatctt gttgaatcat
     1501 acgaatatta tatcaagact atattgaaaa atcaatatat tgtatataaa aagttatcaa
     1561 caactacatg ttgtgcttag gaggaattat gtggggtaaa atcgaagctc tattaattga
     1621 gaaaaaaatg accaaatatg aactgtcaca aaaggctgga ttgaaccaaa attgcttgat
     1681 tgatctaaag aaaggaagaa aaaaatcatt gaaatttgat gatgtcgtta aaattgccga
     1741 tgtcttaggt gtcagcttag acgaatttag ataacaaaaa aagtccgacg gcaatcggac
     1801 tcaaaacaaa ttttaattta cttaattata acataagaga gagggaaaaa ctatggccat
     1861 tgaaatattt ggacctgagt ttagaaaaaa actgcttgaa gatttaatcg ctctaaatat
     1921 ggaagcgata aaaatagcgc agaccaaaaa cgccaagtct attgaatgga taaccatgaa
     1981 gcggctagaa aaagaaactg gatgggggcg aactaaattg acccagtgga gagaacaagg
     2041 gaaatttaac tttaaaaggt catcagaaaa cgggaaagta ctatatgacc tagcagatgt
     2101 taatagattt ttacggacca gtggatatga aaaaggagaa acaacatgaa attattagat
     2161 tttatttttg caaaaccaaa aaaacaggaa ccgattaagc acgacacgct tagagcatca
     2221 tctgaaaagc agtgggccga atttgatgcc tatatgagga aacagtatgg acgacaagct
     2281 taatcagata ttgatagcca tgcgaaacta ccgcacaaat ggtgatgata gcaagtattt
     2341 agaggatatg gaggtcattt taaatgaaaa caaaaattga aatcatgcgt gattttttta
     2401 aagaaaatcc tgaatcaact caacggcaag cctcagaaaa gcttggattt acggaaaata
     2461 cagttaaaca atatatttgg agagatgcta agcgtggcta ctgtattaaa gacgaagaag
     2521 gtcgtgtaac ttatctaaac attgaagatg aattatcgct tattaatgaa tggaaaagtg
     2581 aaattagaag agaattgatt gaacagttac tatcagctaa tcgacatgaa acgtcaagcg
     2641 aacaaattcg tatgaacgct aaaacaatca atcagatttt aggagaaatt taatggaagg
     2701 tatgaaaaag tatatcaatg gtcagattaa actaattgat actattgaag aatgtgtcaa
     2761 agaaaaatgg cctgaatttg atgataaaaa agtacataag atatctttag ctatttacca
     2821 aggatggtcc cttacagata ttaatagcac actcgaagcg atagaaatgg acttggatag
     2881 aaaatgagaa tttatgtaaa caaaaaaggt aaaccatctg ttgaatttga gtttgaagat
     2941 cgacgcggtg gcatgtttga tacaagatta atgttaaaag aatcaccact caaagaagaa
     3001 tttaaagccg atatgtacaa ggcaattgat gatgttttaa aaaaatacga agacattttt
     3061 gatactccta tttttaaaga attaataatt gggaaagaag aagatattag aaaaatgatt
     3121 gattacgatg ataaatttac cgaacttttt ggagggatta gacattgaag attactaaag
     3181 cgacagaaat tacaaaaacc cataattggc gcatattaat ctatggtaaa cccggaaacg
     3241 ggaaaactta cttaactaat tatttaaaag gcaagacact gattttagac atggaccatt
     3301 cgtctaaaac gattgccgga aacgaaaaca ttgatattat ccaatttgac aggacgcacc
     3361 ctagcgactt tatgaccgaa ttcttgacag aactaccaga acttatcaaa gaatatgaca
     3421 atttagtcat tgataatata acaagttggc aatcagactg gtttatcgaa caaggtcgta
     3481 aatccaaaaa tggaatcaca aacgaattgc aacaatacaa catgtggacc aattactatt
     3541 tacgagtatt gactaccatt tacagccaac ctactaatat ttttgtgact gcttgggaat
     3601 caacgcaaga tttaacgctc gagagcggac aaattatcac gcaatacgtg ccagatatcc
     3661 gcaaacaagt cctaagccaa acgctaggtt taaccgatgt tgtcggacgt attcaagtaa
     3721 atccaaaaac gggcgggcat ggaattttgt tgcagggcag cgacggacta tatgcaaaaa
     3781 ataggcttga taataggacc gtttgtaagg cggaagagtt gtttaatttc gaagggagtg
     3841 atgcgtaacg gtataccatt tacacgaata tcaaacagaa cttataaatg aggcaagaag
     3901 acatatttta aagcataatg tgatgattgt tagtccacct ggaagtggta agtcagtagt
     3961 catttctgat attgctaagt cagcgactca aaaaaacgga cacgttttat tcttggtcca
     4021 tcgcaaagaa ttaattgacc aaatcactaa tagttttaaa ttccatggaa ttgacatgaa
     4081 taaagtggat ttaatgacag ttggtaaagc taaaaaccgc ttggataaat taacaaaacc
     4141 aaccttaatt atcacagatg agggtcatca tgggaaagct agtacctacc aaataatcta
     4201 tgaatacttt tcagacgtgc cgaggattgg ttttactgcc acaccttggc gtctatcagg
     4261 cgacggtttt acagacactt acgatgttat ggttcttggt aaaacggtcg agtggcttat
     4321 caataataat aaacttgcac catatgatta ttacagtgtt ctatcaattg atactgcgaa
     4381 attaaaagta caaaacggag attactccaa taaatcgatt gacgaatcat ttggtaaaaa
     4441 gatttttggt gatgtagttc aagaatatat aaaaaaagcg aacggtcaga aagctatttt
     4501 atacgctcac tcggtagaag catcgcaggc atttgctaaa gaattccaat ctatgggaat
     4561 taatgcaata cacgcagatg caaaaacgcc taaagccaaa cgggataaaa tcatgaaaga
     4621 tttccgtgac ggtaagatac aagttatctg taacgttgat ttgatatcag aaggtttcga
     4681 tgtcccagat tgtacagtaa ctattctttg tagacctaca aaatcattag tattattttt
     4741 gcaacaatct atgcggtcga tgagatatca acctaataaa aaagccatca ttttagatca
     4801 cgtaggaaat tggaatattc acggcttacc tgacacaccg catcattggg agaattattt
     4861 ccgaggaggg tggaagaaga agtcgaataa aactaacacg gtacacgcaa aagaatgttc
     4921 tgtgtgttcg gctttgtggc cacttagtca acagctctgt gaattatgca atcatgattt
     4981 tggattgaaa gaaaaacaag agaaagaacg catagaggca gaacttgaac tcataaaacg
     5041 tgagcggttt agaatcaaac aacttgctaa taagaagttt ggtaaagatt taaaaacaaa
     5101 ctgggaaatt gcccaagcta gagttaaaga cgctggtaaa ggaaaaccat tatataaact
     5161 tatctatttc tacttaaaaa ctgattgggt agaaacaaac gttaatgaac ttgccgaagt
     5221 aacaggcaag tcagaaaaag aaatatatag cgcctacaat tggctaaaaa agaaattaag
     5281 aggataaaaa catggcagga tttacaacag atttttcaga agttaaagaa cacgcagaat
     5341 tcaaagaaca accatacgaa atgattgtct atgatgcata tgaagcagta aatgaacgta
     5401 acggcaaaaa acgtgttgtt attgactacg ttgttcgaaa cgatatcaag caagaaatgc
     5461 aaaactacca cttatgggat gagcaatatc ccaactcaca aactgggaag tatcatatcg
     5521 gcatcttaat gggtaaagca aaagcacttg gtatcaaaga ggggcaacac tacgatagtt
     5581 ttgaagcgtt tttaaacgac ttcaaaggac gcactgcaaa agtaaccgtt aaacttgacg
     5641 aatataacgg aaataaatac ccgaaagttc gttacgcaaa tcaaagcgat gtgcctaaca
     5701 gctaccacgt atggaaagaa aaagcaactg gatttacaca agcggagatt gaagaagacg
     5761 atctaccgtt ctaatttaga ctgggggata aatgacatca aatgaattta ttgaagcttt
     5821 atcaaaattg acaaccgaaa ccgattgggg agacccaatc ttcggcgagt cagtgcttaa
     5881 agctgaatta cgaaaacact tatttaaaat tgtcccaatt gatcacaatg ggtatatcca
     5941 caaactgttt tattcagaaa tggttaaaga tgaagatgtc atgtattttg tgtcagatgg
     6001 acgaaaaact tatcgctttt tatttggaga tacaattcta aagactgata aacaaggcaa
     6061 tgaatatctc acatactccg ttgaaaataa ttttccacca tttgcaaaac tagttatcga
     6121 ctacattcta ggtgcttaca cgttttttga gaataaactt tatgacattc gatataagca
     6181 atttaaattg attgatgatt ttacacttca gactaagtat ggtttcaaag actctggtca
     6241 cattttagag atactacaag gtatccacaa aacattaaac atccaaccaa tcaattatat
     6301 tgaaccatat cagattgctt gcaaggattt cataatcgac cttgaaaatt ctgaaatcat
     6361 taaccaaccg cctttgcaaa acgtatctta ttttaagtat tacgaagtag actacaaaac
     6421 agcaataaac agtaagtcta ttgcagaaga atatcttgag tacgttattg cagatagcaa
     6481 ttcgttaaac aatgcaatac tacaatctta ttttatcgcc caagtagcgt gtggtgttag
     6541 acctaaaacc aacttcttca tctcaaaatc tggagtaagg actggtaaag ggttaaggca
     6601 tatagcttta tctggtctat tcaataagat tgatgttgag ctagatacat taaaaagtaa
     6661 tggatttgaa gcgttacagg cgtgggcgat gttctcgggt ggagaaatgg ctctagcgac
     6721 ggaacaggga gatattcaag gcaatgcaat ggagcgtgta cttaaaatta ttgcaacaga
     6781 gaaaacacac gttgcacgag caattgggca aaatcagtca atggttaatt taacgagtgt
     6841 tctgtgtatt gacaccaacc gtactgttgc gctgtcggat gaaatgaatg gacgaaaggt
     6901 tttaattcag tttaaagata gaccaaaaac tgaaactgat tatgaaagag agagcatatt
     6961 caggaaatat tggctagcat ttactgactg tgataaaaat ccaaaaatcg atggatgtat
     7021 cggttttctg ctaaattcac tcgagcgttt ccaaaaaatt ggtaaatggt atcaatggaa
     7081 agatgttgaa gtatttaatg atatcgattt agacgaattt caagttgctt taataaatgc
     7141 attacaagaa gttgattttg tacagcgaac tgataacaaa gaagttattg acttatcatt
     7201 acaagtttac ggaaaaagca atcatgcatt aagtaaagcc atatctgaaa ttggtgtacg
     7261 tagcaggtcg aagaaagtta acgggaaaac agtcagaggg tatgaaatcg aaaataaaac
     7321 acgttttgat aaatatatcc ttt
//
LOCUS       DATUYR010000065         7226 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.065, whole genome shotgun sequence.
ACCESSION   DATUYR010000065 DATUYR010000000
VERSION     DATUYR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.065"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            584..673
                     /locus_tag="VCI33_001436"
     CDS             584..673
                     /locus_tag="VCI33_001436"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP2832355.1"
                     /translation="MIIDYCNNKRIKIKRKGLSPLQYKTKSFT"
     gene            complement(711..1634)
                     /locus_tag="VCI33_001437"
     CDS             complement(711..1634)
                     /locus_tag="VCI33_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832356.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1627..2697)
                     /locus_tag="VCI33_001438"
     CDS             complement(1627..2697)
                     /locus_tag="VCI33_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832357.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2706..3632)
                     /locus_tag="VCI33_001439"
     CDS             complement(2706..3632)
                     /locus_tag="VCI33_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832358.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3632..5134)
                     /locus_tag="VCI33_001440"
     CDS             complement(3632..5134)
                     /locus_tag="VCI33_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832359.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMAGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5199..7169)
                     /locus_tag="VCI33_001441"
     CDS             complement(5199..7169)
                     /locus_tag="VCI33_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP2832360.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTVGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKVEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDSAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2246 a         1404 c         1344 g         2232 t
ORIGIN      
        1 ctttttttgt acttttttat ttgcccgcgt tgtaaaatga agtgcaacaa aaaagagaaa
       61 aagccttgaa ctacacccca aacgttagac aaaaaaatct aacgttcggg gtatttttta
      121 tgaaattaag tgatgaaagt acgaaataga catcgttaag aaagaaaaaa atacctacta
      181 tccaccagag ataaagcaag aaataatgga taacgttttg atagaaggcc cgccacaata
      241 ccaagtatca cttgtttatg gcttgttaca tgcaggaact ctcccaagtt gaatagtaca
      301 atataagaaa aacagttata ctgttgagaa aacaagagca agaccatcac aaacaggacg
      361 taaaccaaaa gaaacctgaa gaaatgacta aattagagcg gcttcaagaa gaaaatgaat
      421 atctcagaac tgaggttgct tacttaaaaa aaccgaggaa ctccgtttga aagaagcatc
      481 tctgtaaaga agaagacaaa aaaaccatta taaaaaatgg acaaagacga gttctgatta
      541 gattttcttt taagaacggc tgtattgact cgttctacct accattatca tcgattattg
      601 caacaataaa cgtatcaaaa taaaacgaaa aggacttagt cctctacaat acaaaactaa
      661 atcctttact taaaatagag tagccaactt ttggggtcag tgcatctctt ttacaattct
      721 ttttgatatt tttcaatttc tgcttggttt gcccaaacaa agtgatttgg tttgatttca
      781 accattgatg gcttatctaa tgtataatca tgttggtctg gatgatagac aacaagttct
      841 ttttgacgct ctaaaattgg atctgggata ggcacggctg ataacaaaga ttgggtgtag
      901 ggatgaattg ggttattaaa cagttcttct gtttctgcaa cttctacaat aaccccttta
      961 tggataaccg caatacgatc tgaaataaag cgaacgactg aaagatcatg ggcaatgaag
     1021 agataagtca aacctttttc ggcttgcatc cgtttgagaa ggtttaaaac ctgtgcgcga
     1081 acggaaacgt ccaaagctga aatcggttca tcagcaataa cgaattcagg gttcattact
     1141 agggctctag cgataccgat ccgctgacgt tgacctcctg aaaattcatg agggtagcgc
     1201 gtcaaatgct ctgatagcaa accaacttcg gccatcatgt tcttaatttt ttctttacgt
     1261 tcttcctctg ttttaaacag attaaagtta taaagacctt ctgaaatgat gtagtcaacg
     1321 gttgcccgtt cattcaaact agcagcggga tcttggaaaa tcatttgaat tttacgaatg
     1381 agctcattgg cttctgattt tgattttctg ccattaatta ctttcccatc gtataaaatt
     1441 tgacctgagc tagtatcgtt caaaccaata atagcacgac caattgttgt tttaccactc
     1501 ccagattctc caactaaaga aaaggtttct ccttttttaa taaagaaatt agcattttta
     1561 actgcaacaa attttttctt tccttctccg aaggaaattt ctaggtcttt tacttcgact
     1621 aatttctcag acatttcctt cctcctgttg tgacattttc cttaagattt tttgatgcaa
     1681 atcttgaatg acttctggtt tttgaacttt tggagcctct gggtgtaaaa gccatgtttt
     1741 ggcccaatga gtctcagata cgttaatcgc cggaggtgct ttttcaaagt ctaaaaccat
     1801 agcatattct gagcgaagtg caaaggcatc tccgataatt ggtgaataaa gtgatggagg
     1861 cgttcctgga atagcgtaaa gttcaccaga ttcatctgcc aactgcggta agctagacag
     1921 caaactccat gtataggggt gccttggatc atagaaaatc tcttcgactg ttccaaattc
     1981 aacaatttct cctgcataca tgacagccac tttatctgca atgcttgcca caacacctaa
     2041 atcgtgcgta ataaagataa tggtgaaatg atattctcgt tgaagcgact tcaataactc
     2101 aacgatttga gcttgaatag tcacatcaag ggctgttgtt ggctcatcac agataagaat
     2161 atctgggcga caagctaaag caatagcgat aacaatacgt tgacgcattc ctcctgaata
     2221 ctcaaatggg taatcttcaa agcgtttttt ggcatttggg atacccactt tattcatgta
     2281 atcaagggcc atttctttag ctttggcatg acttactttt tggtgcttaa taatcacttc
     2341 tgtgatttgg ctaccaatag ttttaatggg actaagactg gtcattgggt cttggaaaat
     2401 cgttgcgatt tttgagccgc gaatctttgc ccactcttta tttgttttta aatctgtcaa
     2461 ttcttgccca cgatagacaa ttgaaccatt agcaatgcgt ccattagact ccaacatccc
     2521 tgtaaaggtc tttgttaaaa cagatttacc tgagcctgat tcccctacaa aagcaaggac
     2581 ttctccttca acaagttcca gtgagacgtt acggatagct gttaaaacac gatcacgcac
     2641 atcaaattct accaccacat ctttggcagt taagattaca ttattttctt ttgtcatttt
     2701 aactcctatc tatgtgaacg tgggtcactg gcatcagcca agttttgtcc gacaatgtat
     2761 agtggtaacg atactaaaat caatgttact aagggaatcc aaaagaggta ggcatttgtt
     2821 gttaagttgc ttgaataatt agcaatcaaa cgtcctaaac ttggagtggt ggttggtaaa
     2881 ccaatcccaa agaaggataa gaaggcctca gaagatacat aaactggtag catttgtgac
     2941 aacatagtca tgataactga aaccaattga ggcaggaggt tcttaacagc aatcttgtac
     3001 attggtgttc ccaaagtttg actagcaagg ttgtattcta aatcacggta acgcaagatt
     3061 tgaacacgga tggagtaggc gacaccaatc catccagtga tacagaaagc tagaatcaaa
     3121 ttccaaaatc ctgcacctaa tgaataggtc aaaacaatga taataagcat agaagggata
     3181 tttgagataa tgttataaat ttcaatcata actttatcaa atgctttaga aactccccat
     3241 atggctccta acactacccc aatggtgata ttaattagtg tcgctataac tgagattaaa
     3301 atagaattac gtgccccata ccaaacacca tcaaacagag attgcccatt tttgtcggtt
     3361 ccaaaccagt actctgcatt tggccaaata taacgctttg aaaagtcatt gatattacta
     3421 acgtcattaa agtcgtagtt ggcaaacatt ggataaataa agctcatcat caagactgtc
     3481 actaaaatca cgagcataaa gactgtagat tttttagaaa aaaactgacg aaacactgat
     3541 ttccagtaag aataagcagg ggtatcaatc acttcggagg cttcactatc gcgctcaaca
     3601 aatcgaaatt tagatttatc aatcgattcc attacttacc tccttttttt gttgacaatt
     3661 taatacgtgg atcgacaagg gtcatgacaa tatctcctag taaaagtgaa acaattgaca
     3721 agacagtgaa gataaaggtt aatccaacaa tcatagaatt gttagcagat ttaatagaat
     3781 caattaacat tttacccata cccgggaagg caaagacagt ctctgttaag gttgcaccac
     3841 caatagcaag gataatactt gcaggaaccc ctgacacaat tgggaccatg gcatttttga
     3901 agagatgccc tctgtaaatt tcagattctg ataatccttt tgaacgcgca aaacgtaccc
     3961 aatcactggc ctgaaggtca acaagatagc gacggaacca gataaccgta gttggaatat
     4021 ttaaaattcc taaaataagg gctggtaaaa tataagattt aggatcagat gcccctagca
     4081 ttgggaaact atctggtaaa ccagccatgc cacctaggaa gcgaacaaca tagataacag
     4141 caatacttgg taaagcaagc atgaaggtca tggtcgctgt tgagaaacgg tcaaaatagg
     4201 tatttttaaa ccgagccata aataaaccaa gaggcaagcc aacgatatag gaaaacataa
     4261 caccaaaaaa accaattaca aaagaattgt gaatcattga tgggtcgaca taattattga
     4321 tggttttagt gtaggaatct cccttaccat agtttacctt ggtgatatca tccaatgatt
     4381 ttggattttt gtagctacgg ctatagatat ccactgacga tttcttagtg acacctgatg
     4441 ggaaggtcac atcttgaaga gctgttcgtc cttgcccttg tgaaataacc tgaagaacag
     4501 gaatattgct gtatgttggg taagatgttc ctaagtttag ggtaacaaag ttttggtgaa
     4561 gataaggaaa ttttccgtta gtgtagagga gatatttatg ttttgtcccc gaaccaacaa
     4621 gtgaccagcc aactgattta tctttttcta gacgaatata cctagctaat tttggattgt
     4681 ctttatcctg aatcttccaa gggtgatcaa taacaactag atttgagaaa aagttccaaa
     4741 cacgttcgta aataggaata ttgcgaattg cataaaactg tttgctagtg gggaagcgtt
     4801 ttagctgcca gccattgcca agagaatcaa tatacttctc atagatagct ttgttagcac
     4861 tagttgcttc tgtcgttaca ctactatcta ctttagaagc attatctttt aattctttgc
     4921 ttgaaaaata attgacataa cccatacgtt gaaaagttag attttcatac gctacttttt
     4981 tatctggtgt tgttgtcatt ttattataat tagggtcttg tttaaagata aggctggtag
     5041 gtactagagt atatacgatg gtgtatgtta aagccgttac caaaactacc gacactaacg
     5101 atcgcatgat acggtttaaa atgtattttt tcatagttat cctcaatagt tcaaaagaac
     5161 ttcctcacat aaacgagaaa gttcttttga acatcgtttt atttttcaac gtgatcagct
     5221 aacttctcag catattttgc atttgactta gcctttgctt tcatccattt ttcttttgca
     5281 ttttcgtatt gttttgctgt gacaggtttg tcttgaagtt tcattccttt atatgctaga
     5341 ggacctttag accctgccca agagaagccc ccgctaaatg gaacggcttt agtaactcgt
     5401 ggagtgccac ccaatgccac aactggaata tctacggcac tatctgtaag gtaggcttgt
     5461 gcttttgcgt aagctttata gcgcgcatcg ttgtcgtctg taattgctgc tgcttcatca
     5521 aggagagttt ggtaagtatc aaggcctgca gctgccacaa catccttatt ttgacctgct
     5581 ttgattccaa gtttttggat aacagatcca ccacctactg gactcatgat gtcaaggtag
     5641 gtccgtggat cttggtagtc tggtccccac catgatgaaa tgatatcgta gtcttgttgt
     5701 tctggggtct cagcatagaa gccttgggct tcgtgagttg atgtttctgt ttcaagaaca
     5761 ttgacaatga cattttcttt accaagagat gcttcaacag attgtttgaa agactgggct
     5821 tcctgaacag ttgctgcgtt tgcctggtca acagggtaat ctaattgaac tgggaaggtt
     5881 acaccttcag ctgtcaaagc ttctttggct tttgcaaatt caacttttgc tttttcagga
     5941 ttatagaaac catcttgagc atcagctaag ttaacgtctt tccattcatc accaagtttt
     6001 gccatttcct tttcaacttc tgaaccaaaa tcactttctc caacggttac aaaagttggt
     6061 ggcacaagca tgttacgtaa ggcttttgtt ttggcatctt gacctgcagt ttgtgcttgg
     6121 aatgacgctc ggtcaaaagc aaactgaata gcttgacgaa aatccttgtt gttaagagct
     6181 ttcttaccgg catcttgttg tgcagggtct ttcttagtgt ttttgaaaga agtacggttc
     6241 aaattccatg ttaaatgacg gatatctcca gtcaacattc cgtaagtaat attatcagca
     6301 tagtttttct tagctgattt gtaggtaggg tcatttgggt aaagtcgtgc aacgctgaac
     6361 tcacccttgt caaagttctt gtagaacgaa cctgggtctg aaccatctga gtaagtcaat
     6421 ttaacagatt ctatcccaac attcttagca tcccagtagt tttcattttt atggaattcc
     6481 atagatgatt ttgaggtgaa ggcgctcaag aagtaagcac cattaacaag gattgatgat
     6541 ggatcggttg taccaaaatc tttacctttt gacttcaaaa atttcgcatt aactgggaaa
     6601 agcacactat aagttgtttt tgaattccag tagctttcag gcttgttcaa agtatactga
     6661 acagttttat cgtcaagggc tttgacacca acttctttaa aatctacttc accattttgg
     6721 taagccttta agttttttat tgaatcttca acaacgtaaa gagcatctga tttatcgtca
     6781 accgcgtgct tcaaaccagt cacaaaatct tctgctgtta ctggggcata ttcttcacca
     6841 tcagccgtat accaagagac accatcgcga agagtataag tataagtcag accgtctttt
     6901 gaaaccttcc aatctttagc aagtgatggt actaaattac catactcatc gttttccaaa
     6961 agaccatcaa ccatttgtgt tatcacatca gtcgttccac cgccattagt caaaatataa
     7021 tccaatgatt ttggatcgtt aacaaaaacg tacttgtagg tttttgtagc ttctgagcca
     7081 cctgaagcat ttttattacc acaagctgcc aaagcggata ctgacaagat cgcaacactt
     7141 acagctgcca accatttact tttcttcata aaagtcaccc ctaaacttta tttttcattt
     7201 tttggcaatt gctaaatgaa attggt
//
LOCUS       DATUYR010000066         7198 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.066, whole genome shotgun sequence.
ACCESSION   DATUYR010000066 DATUYR010000000
VERSION     DATUYR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.066"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            62..223
                     /gene="sagA"
                     /locus_tag="VCI33_001442"
     CDS             62..223
                     /gene="sagA"
                     /locus_tag="VCI33_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEP2832361.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            445..1395
                     /gene="sagB"
                     /locus_tag="VCI33_001443"
     CDS             445..1395
                     /gene="sagB"
                     /locus_tag="VCI33_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEP2832362.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1392..2450
                     /locus_tag="VCI33_001444"
     CDS             1392..2450
                     /locus_tag="VCI33_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEP2832363.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPEMFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNAPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     ADVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2470..3828
                     /locus_tag="VCI33_001445"
     CDS             2470..3828
                     /locus_tag="VCI33_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEP2832364.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3803..4474
                     /locus_tag="VCI33_001446"
     CDS             3803..4474
                     /locus_tag="VCI33_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP2832365.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKGVT"
     gene            4471..5154
                     /locus_tag="VCI33_001447"
     CDS             4471..5154
                     /locus_tag="VCI33_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEP2832366.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIAILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5177..6100
                     /locus_tag="VCI33_001448"
     CDS             5177..6100
                     /locus_tag="VCI33_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832367.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6109..>7198
                     /locus_tag="VCI33_001449"
     CDS             6109..>7198
                     /locus_tag="VCI33_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832368.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFII"
BASE COUNT         2239 a         1109 c         1388 g         2462 t
ORIGIN      
        1 aggttaaaaa taaacgactc gcgttcttat cagttactta ttagataagg aggtaaacct
       61 tatgttaaaa tttacttcaa atattttagc tactagtgta gctgaaacaa ctcaagttgc
      121 tcctggaggc tgctgttgct gctgtactac ttgttgcttc tcaattgcta ctggaagtgg
      181 taattctcaa ggtggtagcg gaagttatac gccaggtaaa taatctattt agcatctcta
      241 tgtgatagtg atattaaggt aatgagtttg ctagcaacta agttatcttt ggtaacttct
      301 gattatagat gaatggcata gaggtgttag aaaacatgag acaaaagtaa tcttcttgat
      361 gctaacgtct caggtaatta gcaggtacta gatagtacct gctaattact atatgtttag
      421 taaaatgaga taggaaagat agttatgtca ttttttacaa aggaacaaca acctaaagag
      481 aattgtccgc caataactgt tgaaaaagca aggcaattgt ttgaatttaa tacaaaccac
      541 ttgtccttat cggattacca tcatcaaacg gtgctaaaaa cgtcaaagca gctagttgct
      601 caacatttaa tgcctaatgc aaccgataat cttagtcaac attttttgat gaactataaa
      661 gctaataata attatttagg cttccaagct agtattgtcg acttttttac agattctgcc
      721 gttgctaatt tttcaagtag ttacgtttat gaaagtcagg aaaagataat tcgtttacca
      781 aaacctacca agatatcaac tgctctgtcg acatgtatta taaaacgaag aagtcatcgt
      841 caattttcag atagacaaat gcctcttcaa gatttatcaa acattcttta ttatgcatgt
      901 ggtgttagtt cacaagcatc aattagagat ggagcatcag ataagattac actcagaaac
      961 tgtgcttcag gtggaggttt ataccctatt catttagttt tttatgctag aaacatcagt
     1021 aaattaatag atggtttcta tgaatatcta ccctatcagc atgcactaag gtgttatcgg
     1081 catagctctg aggaaaacgt tagagatttt gcggaatacg gtgctattaa tgctgaaaat
     1141 tgtaatatta ttattattta tgtctaccat tacatgaaaa atacacgtaa atatgggaat
     1201 caggcgactg cctatgcttt tattgaatca ggagaaatag cccagaatat tcaattgact
     1261 gcaactgcct taacttatgg aagtattgat attggtggtt ataataagga atatctccaa
     1321 gaattattag atttagatgg gctaggagag catgtgattc acatgacact cgtaggaact
     1381 aaggagtctc aatgaaatat caacttaata gtaatgttcg tgtcgtgaca tttcaagata
     1441 cattttgttt tagaaaagga atctgggatt ttaatgaagc cattctagat ttgacacaag
     1501 aacctcaaaa tttaaagata gtttatcaag aaatagtttc acagttggtt aagggagtag
     1561 ccattgatac tgaaacttat gaaaatactt tagaacctga gatgttcgct aatttaatgg
     1621 aagtcatttc aggcttgtat tacaatgata tgctgatgct tgaggatgac tacaatcttg
     1681 aagaaaatgt catgaaaatt ttaatgggta atttccgttt tatggcgcaa gcaggtcata
     1741 tggtaagcaa tgctcctgtt ttgtttatca gtgattcgac ttatgttaat gaatctgctg
     1801 agttgctagc tgaacaactg catttacaat tgcaagtggc tagcgatgac ttaaaaatgt
     1861 taattcagca ggcagatgtt agttcgcgtt tagatgcttt ggagcatcgc cgtaacatga
     1921 attgtttatc agaagccttg tgcgattacc aaagtattat tatttgtcaa gaacgcttga
     1981 atattatgat gttgcgacac ttgaatgaag ttagcgtagc tatgaaaaaa caattagtga
     2041 ttggttttgt agatggacca ttcttgcaca cttgtacttt gaatcctcct catagtgctg
     2101 actttgatag cttagaaaga agagttttgg cacgtttaca agactcaact ctctatcaac
     2161 attttgctaa tcagatttta ccagcaacac aagatgtgag ccaggcctat ttaccattgt
     2221 taaatgtttt aatgaattta gtcgttagcg aagctttcat tattgctcaa acaggtagtt
     2281 ctaaatttga gggacgttta ttaagcattt acttaccgac gcttgagatt caggtccaag
     2341 atattttgaa aatgtctaat tcacagacac aaggagcttt ggcaaaatta aaatatgagg
     2401 atcagcagat atcaacacgt gagattgtta aaaaactcct tgacgagtaa cctataagga
     2461 gaatctatta tgttatacta ttatccttct tttaatcata tttttgatga attaaaaagt
     2521 ttaagtggca atcgaacagg tatcttaaat caatctcaag ttccagtttg caaccatcct
     2581 cacgatgttt acttaaaaag tattactggc caaatgccag attatcataa acaatttata
     2641 ggtgagttga gtcaagttag ttatcacatt attggttacg gcagttacta tgaagaagca
     2701 cttatcaagt atttaggaga aagtattgaa cgctatgcta ctgtaattgc cggagactta
     2761 ctatcagatc ggattgtcta tgcttcttat aatgagttaa aactgttaca taaagtgatg
     2821 cctttagaat acttgcaagt gtttactcaa gaacagatcg cattatcttg tgacttgcaa
     2881 atgatgatgt gcgataaaat ggtaactgag aacgatgtat tgggctgggt taaatgtccc
     2941 atgttctttg aggacgcaga gatgtatgtt ccagctcaaa tgctttgtgt tgggtataaa
     3001 acaaatgaga ctgtaggaga aagacggata ataccaggat tttctacggg aacagcttct
     3061 cacaaaacgt tagaagcggc tatgtgcaac agtttgattg aatacatcca gatcgattca
     3121 atgatgctta gctggtatac taagaagcct tgccccaaga ttattgttga tgatcctgat
     3181 attgaagtga ttttagaaga agctagactg ggaaaagaca gtctatacga cattattcct
     3241 attgatatga ctgtaggaga agataatcct ctttatacgt ttggaatcat tctcaaaaat
     3301 aaatatgatg aagggcctta cctcttgttt ggagtgcagg ctgggttaga tcctaaacat
     3361 gctttgctaa gagggatcat ggaagcttca gctattagct atagttacta ctataatctt
     3421 ttatatcaaa aagcttccct tgcaaatatt gaatgcgaag aacctttgtt tttagattta
     3481 gatagtaatg tcttctatta tgcacatccc aaggaccaag accataaatg gaaggccttt
     3541 gaacctttga tttcaggaga agttctgtta agtgatttgg aagatcattc tggtaaagat
     3601 aaaaaagaag atttgaagac tttattagct tatgctaaga aagtcagtcc taatgctgtc
     3661 tttttagata ttacacctcc tgaagctcta gaaaaaggct ggtacgtcac gcgagttctg
     3721 atgccagaac ttcttgaaat gtgtatacca gcatttcctt ttgctaatca tccacgaatg
     3781 agacagtttg gaggtgtaac aaatgccttt gtccatccaa tgccttaatc tttgtttttt
     3841 gttggtaact ttttgtcctt caattcctat gcaagctatt tttggaaagg aagatagtgg
     3901 ttatgcattt aatttaattg gttttctaag agcaactctg atttatgaca ttttggcttt
     3961 ggtaagtatt tatgttttgt cacctcaaat aaccttgtct cttgaaagta ttgatagtaa
     4021 gacgttcttt atgggacttg tattttgcgt cttgatagta ctgattgaac tcgttttctt
     4081 acatggttta cgttgttggc aaaaaaagca atggcttcct gcaactttct cgtttgtagg
     4141 aacgacaaat gattggtcta agattggcta tcctttatta ctagctttat ttgaagagac
     4201 gatttatcgt tttttgtggt ttaatatatt agcttttcaa tggcatttac caacaattat
     4261 tgttttaatt gtaactagtt tttgctatgc tttaaatcac ttattgatgg ggaaatccat
     4321 tttttatgct aagttggtaa caggcatcat ttatggtagt atttacatgc tgactagtca
     4381 attgtggttg gttgtgataa tgcatgtagg agggaacttg ctagttgagt gtcttagcca
     4441 tcttcaaaca aaaaagaaga agggggtgac atgatgctat tggttttgct gtcgttttta
     4501 ggtctaacta ctctttggtt gaccagttat cgaagacgtt gtttggctag atggcatctt
     4561 caatggttag tggccttatc ctatcaagat tttttagatg tcttactgag tctgtttcaa
     4621 tttgtcatta ttatacttgt tttattcttt tactcagcta ctattaatct aggagaggtt
     4681 ctgacctttt tgacacaaac tagctggcat tggcagattc tttgttattt agtgttgtat
     4741 cttatggcca tcatagagat gacgctttta gttttaattc ttatctttga tgttttgctc
     4801 cagaaagata gtcgattgct ctttaaaaaa ataacttggc tgccttttag acaagataaa
     4861 ccagtcttaa gtatactttt actaggcata gctattctag tagacagtct cttttatttg
     4921 ggcttgctta tattattagg agaacagtcg ctaacaagtt tagcaacgct tattttaggt
     4981 tatgggatag tcaaggcttg tcgctattca ggctggttaa atgtactatt agctttttgt
     5041 cttttcacta tagttggact atgggcagtt acggcaactt tattatatgg ttggctagta
     5101 ggtgcgatga tattaacggt gacttatttg atgattagtt gcaaagagta ttagcaatat
     5161 taaaaaggag tgatatatga gttttgtaca attaacaaat gttgtcaagt cctacaaaaa
     5221 cggcaagaaa gctgtcaatg atgtttcctt gtctattgaa gcaggtaata tttatggtct
     5281 gttaggacca aatggtgctg gtaagtctac cttgattaat cttatcttag gcttgatccc
     5341 tttgagttcc ggtaaaatta ctgttttagg gcaatcccaa aagactattc gaaaaataag
     5401 ttcgcagata ggttatgttc ctcaagacat tgctgtttat ccagacctaa ctgcttatga
     5461 aaatgtagaa ctatttgggt cactttatgg cttaaaggga gctcagctta aaaaacaagt
     5521 tctaaaaagt ttagaatttg tggggctaca ctcccaagct aagcagtttc caagtcaatt
     5581 ctcaggagga atgaagagac ggttaaatat cgcttgtgcg ctagttcatt cacctaaatt
     5641 aatcattttt gacgaaccga ctgtagggat tgatcctcaa tcacgtaatc atattttaga
     5701 gtcgattcgt ttgctaaaca aagaaggtgc cacagttatt tatacgaccc attacatgga
     5761 agaagtagag gctctttgtg attatatttt tattatggat catggtcaag ttattgaaga
     5821 aggacctaaa tttgaactgg aaaaacgtta cgttgcaaat ctagcaaacc agattattgt
     5881 aactctaaca gattcacgtc atttggaact ggcagataag cctgattggt ctttgataga
     5941 agatggagaa aaactcatgt tgaagattga taatagtgat atgacatcag ttgttcatca
     6001 gctcacacag gccaatatta cttttagcga gattagacat aaccatttga atttagaaga
     6061 aattttctta cacttaacag gtaagaagtt acgagattag aggttactat ggttttattt
     6121 catttaatca aaaaagaaag tttacagatt tttagaaacc gaacagcctt attgatgatg
     6181 gtgatttttc caattttgat gatcgttatt ttaagttttg cctttaaatc aagttttaat
     6241 actgcgacaa cagtccctaa attgaccatt cgttaccaat tagagggtga aaaaacggat
     6301 taccagaaaa attttcttgc ttttttaaaa gttttaaacc aaaaacttca tttagaggct
     6361 aaacctagta attctcttga aaaagatcga caaagggtca gtgaaggagc cttaacggct
     6421 gttttagaag tgaagaagaa tcagaccatt aaggttatta ctaataatat taatcagcaa
     6481 aatgcagatt tgatcaatat gctagtaaaa aattatgtag ataatgctaa aacttatgac
     6541 tcgatagcag ctctttatcc tcaacaatta aatcatatca gaaagcgaag tgtggactat
     6601 gttaaggtca gttcaataca gacaagtaaa ggaatgacat cagctgatta ttatgctatt
     6661 tctatgttta ccatgattac tttttatagt atgatgtctg cgatgaacct tgttttgtca
     6721 gatcgtcaac aacggattac aaatcgtatt cacttaacag gagtttctcc aagtcttttg
     6781 gtctttggaa aattaatagg tgctatgtta gcaacaactg ttcaattgag tcttttatac
     6841 atttttacaa ggtttgtttt acgagttaat tggggcacta atgagtggat gcttattggt
     6901 ataacagcct ccttagtcta tctctctgta gctataggta tcgggctagg cataagcatt
     6961 aaaaatgagg cttttttaac ggttgcatcc aatactatta ttcctatatt cgccttttta
     7021 ggaggcagtt acgttccatt gacaacatta cacagctcta ttattaatca attgtcgaat
     7081 atatctccta ttaaatgggt taatgatagt ttgttctatc ttatttttgg tggtcaatat
     7141 aatccgattc ctgtaacttt gatcgttaat attagtatcg gaacaatttt tataatat
//
LOCUS       DATUYR010000067         7060 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.067, whole genome shotgun sequence.
ACCESSION   DATUYR010000067 DATUYR010000000
VERSION     DATUYR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.067"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            140..838
                     /locus_tag="VCI33_001450"
     CDS             140..838
                     /locus_tag="VCI33_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2832369.1"
                     /translation="MFSGHQLKTARLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
     gene            complement(973..2535)
                     /gene="ffh"
                     /locus_tag="VCI33_001451"
     CDS             complement(973..2535)
                     /gene="ffh"
                     /locus_tag="VCI33_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEP2832370.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQVFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(2550..2891)
                     /locus_tag="VCI33_001452"
     CDS             complement(2550..2891)
                     /locus_tag="VCI33_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEP2832371.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            complement(2980..3678)
                     /locus_tag="VCI33_001453"
     CDS             complement(2980..3678)
                     /locus_tag="VCI33_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2832372.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            complement(3761..5194)
                     /locus_tag="VCI33_001454"
     CDS             complement(3761..5194)
                     /locus_tag="VCI33_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832373.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            5361..6923
                     /gene="guaA"
                     /locus_tag="VCI33_001455"
     CDS             5361..6923
                     /gene="guaA"
                     /locus_tag="VCI33_001455"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEP2832374.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
BASE COUNT         2122 a         1344 c         1279 g         2315 t
ORIGIN      
        1 agtcgctatg ttttaggtgt ttcccctttt tgacagtatt gctatcacat gaattaattg
       61 taggaaatgt tacacttgct ttcgttttat ttttttgata ctattagtaa agttataact
      121 ttactaaagg agctaccaaa tgttttctgg acatcaacta aaaacagcac gattatcaaa
      181 gggaataact caatcagaat tgggaagatt gttgcatgtc aataaaatga caatatctaa
      241 ttgggaaaaa ggtaagaata taccaaatga aaaacattta aatgccttat tgcatctatt
      301 caatgtgaca tctgattatt tcgacccaaa ctatagattg ctaacgcctt ataaccagct
      361 gacaatatct aataaagaaa aagtaattgg ctattcagag cgattgttaa atcatcaaat
      421 agacaaaaaa tctaaagatc tcatagataa accatcacaa ttatatgctt atcgggtcta
      481 tgaaagttta tctgctggta ctggttactc ctattttggt gatggtaact ttgatgttgt
      541 cttttacgat gaacaattag aatacgattt tgcgtcttgg gtttttggag attctatgga
      601 gccaacttat ttaaatggcg aagttgttct tataaaacaa aatagttttg attacgatgg
      661 agcaatttat gcagtcgaat gggatgggca aacatatatc aaaaaggtat ttcgtgaaga
      721 tgagggatta cgtctagtgt ccttaaataa aaaatattct gataagtttg ctccctatag
      781 cgaagaacct cgcattattg gcaaaattat cgctaatttt aggcccttag aaatttaagt
      841 tagattgatt aggttatttc aaattttaaa tgaactcatc atgcatgtaa cgcggtcttc
      901 cataatttta ataatcttgt atacaagata aatgaatttc acatcaaaaa gagacgaaaa
      961 tttccgtctc ttttattttc gttttttctt tgcttttttt agtttgttag cttgacgttt
     1021 catggcctgt ttcatggcaa attgtccaat tttccccttg aaccctttac caaatacttg
     1081 actcatatcc atatcaccac caaggccaga taaatcgggc ataccacctt gtcccatcat
     1141 cccttctaag gaagacatat ctggcatacc agcaggcatg tttttaggaa gattattggg
     1201 attaatgccc atatctttca tcatcttaga catatcgccc gacataaccc cttgcatcat
     1261 gcttttggct tggttaaagt ctttgataaa tttattaact tccacaaagc tgttcccgga
     1321 accagctgca atacgacggc gacgactcgg gtttagtaag tcagggtttt cacgttcagc
     1381 gggggtcatc gaagagacaa tagcacgttt gcgagcaatt tggttttcgt caactttgat
     1441 attggccaaa gctgggttac cagccattcc tggaatcatt ttcaagaggt cttccatagg
     1501 ccccatattt tgcacttgat ctaactgttc aatgaaatca ttgaaatcaa aggtgttctc
     1561 acgcattttt tcagctaact ctaaggattt tttctcatca tattcctggc tagctttttc
     1621 aataagagtg agtaagtcac ccattcccaa aatccgactg gacatgcggt ctgggtgaaa
     1681 tgtttcaata tctgtgattt tttcaccaat acctgtaaat ttaattggtt ttccagtgat
     1741 ttcacggaca gatagtgctg cacctccacg ggtatcacca tcaatctttg ttaacacaac
     1801 tcctgtgatg ctcaactgat gattaaactc gtaggctaca ttggcagctt cttgaccaat
     1861 catgctatct acaaccaaaa gaatttcatt tggttgtgct aaagccttga catcgcgcag
     1921 ctcttccatc agtttttcat caatttgcaa gcgacccgca gtatcaatca agacatagtc
     1981 attgcggttt tcacgagctt gttccaaacc ttttctgaca atatcgactg ctgagtggtc
     2041 tgtccccata tcaaaaacag gtacattgat ttgttgcccc agtgttttta actgatcaat
     2101 ggccgctgga cggtaaatat cggcagcaat catcagtggt cgcgcgtttt cttctttaat
     2161 taatttatta gcgagttttc ctgcaaaagt ggttttacca gccccttgca aaccgaccat
     2221 catgataata gttggaattt ttggagattt atcaatctcc gcggtttctg agcctaaaat
     2281 actcgttaat tcttcattaa caatcttaag aatttgttgt gttggatcaa gagtatcaat
     2341 aatctcatgg ccaatagcgc gctcacgtac gcgcttaata aaggttttaa caactggtaa
     2401 agcgacatcg gcttctaaaa gagctagacg aatctctttt gtcacttctt gaacatctga
     2461 ttctgataat ttctttttac cgcgaatgtg tttaaacaca tcttgcaaac gttgggttaa
     2521 gctttcaaaa gccatagtca aaactcctct tactctctat tgtcaatact ggttagaatg
     2581 gaaatttttt cttgcaaata ctcatcatga gggtagtgag caatcatgtc gtcaaagatt
     2641 tcacttcgaa caacataatc cgaatacata tgaagtttca tctcataagt ctctaaaatt
     2701 ttttccgtac gcttaatatt atcatagaca gcctgacgac tgacaccaaa ttcatcagca
     2761 atctcagcta agctataatc atcggcataa tacagctcaa tataattcat ttgtttatct
     2821 gttagcaaag ctgcataaaa ttcaaaaagg gcattcatcc tgtttgtttt ttcaatttcc
     2881 ataatgttca ttttatcata ctatgattgt ttttaacagt agcaaattaa ttgccttatg
     2941 ctgttcgtgt aaaaaaggaa gtcataaatg acttctttac taattatttt ctaagtaaaa
     3001 ttcaaaacgg tcaccgatat actgactgcg gacatactca aaaggtttgc cgtctgtaaa
     3061 gtaagacacc tgagttagag ccaaaatggc atgtccttta gcaacttcca agtaggaggc
     3121 tacacgctca cttgctagct tagcgtagat tgtctgttta cttttaccaa tctcgtaacc
     3181 atttgctatc aatgagtgaa aaaagtgttc tgtaatatca gctcgtttaa ccgtttttat
     3241 aaatttttct gggattgaag caatttcgta caccaaaggg acactgtctg catagcggat
     3301 acgttccatt cgaatgacca agtctgtttt gtctaagttc aattctttaa cttcggtatc
     3361 actagctaat tgcttttgat aggataataa tttagaggag ggttgtcgtc cttgggagcg
     3421 tataatttct gtgaaacttg ttgttcctcg cattttttct tgaacacgat gactggcaac
     3481 ataagtacca cttcctatcc gcctttcaag aatcccttct tctactagta atgtaatagc
     3541 ctgccgtaga gtcattcgac taacggtgaa atgttccgct aaatgtcgtt cacttggtaa
     3601 gcgactacca attggccaga tgcccagatc aatgtctttt ttaattgcat catgaatttt
     3661 tatataagct ggtaacatgc cacactcctc aagtcggtta ttcatctatt ttatcatatt
     3721 tttgatgaaa attgaattgt aaatattgag gtatttggca tcaattaatg atattacctg
     3781 cgacaataac tggtgaattt ggtggaaaat gaataggttc cataaaccaa ctagtgagct
     3841 tcgctttagt aacaatggat gaggtaaaaa cattatttaa aaccctaata actcctactt
     3901 ttgaggaatg ctgaccaata gaaccactat aggctctcag gcaaccagtc ggatctatat
     3961 aaggtaaaaa ttgattttgg cttacagtaa tattgtgaca tagatgtcga gtttggttaa
     4021 atgcttcata gtttggagca atattactaa gtagacctcc atcccatgca aaatgatgaa
     4081 cagcagcatc aaactgaatg acttctgaaa aaaaatcatg cccttgagcc tttgatagca
     4141 gctgttggtc ttgtactaat tctggcgcat agccaataaa ttggtttttc tcaaacaatg
     4201 aattttgtag agatcctaaa tcaaaaatat gactattacg cttatgaacc atagtaaatt
     4261 tgttatcgta aatatgccaa tcagtaccat gatcagccat aatcataaag tggtctcctt
     4321 ttttcaaatc atttgctttg aaatgcactc cagtcaacac gagattacga acaccttgtt
     4381 cagcctttgg tcctgtaggg aatccaaacc acaacatttt accgtgaata atgaactctg
     4441 tctgatgacc aataatagct gtatcagatg gaagctcaaa ataatctttt tgtgaaggca
     4501 aagagccaat gtgaaattgc ccctttggaa aagatataac gctatttgga tgctgctttg
     4561 cataagcgaa agcttttcgc aaaagacgat tgttttgctc aggggaattt cctttattga
     4621 acctaatata ataaggttta acaaaatctt gctctgaaat tccatctaat cttagattat
     4681 ctttcaggtt aatatcgtac tgatatcctc ctaacgaata gtcatagcgt tttttaactt
     4741 gtttaatagt cgctaattga ccttgataca cttttatatg tggcttttta gttaatgaag
     4801 ataatttaac gatatctcct tttttaaacc tcggtttatc aggctctttc aggttatgtt
     4861 ctggcaaaga aacagtgact tttcctattg gaaataccac ttcataatga taactcactc
     4921 ggtaattttc ggctatatct tctacctgtt tattaatagt tccaatccat tgatgatatg
     4981 gaccactttt ttgtacttga atagcttgtc caatgtgata gcgaggctgt tgatctttgc
     5041 ttcgttccac tattccgaca gttgctaata tattgtgaag tgttgcaaaa ctattggtcc
     5101 acgcaaggta cgaaacaact aaactcaaca ataatcctag gaaaattaat tcaacaggtc
     5161 gaaataaacc caaaattctc ttttttctta gcataggccc tccctgttgt cttaattccc
     5221 tttcatagcc tctattgtaa cataacgatt acgttttttt ccaacgagct agagaaattg
     5281 taatgtcttt tcgatgaaaa tatgctacaa taagttgaca actatcagcc tgacggttgg
     5341 tatagaatga aaggattata atgactgaaa tttcaatttt gaatgatgtt caaaaaatta
     5401 tcgttcttga ttatggtagc cagtacaatc agcttattgc tagacgtatt cgagagtttg
     5461 gtgttttctc cgaactaaaa agccataaaa tcaccgctca agaacttcgt gagatcaatc
     5521 ccataggtat cgttttatca ggagggccta actctgttta cgctgataac gcctttggca
     5581 ttgaccctga aatctttgaa ctagggattc cgattcttgg tatctgttac ggtatgcaat
     5641 taatcaccca taaattaggt ggtaaagttg ttcctgctgg acaagctggt aatcgtgaat
     5701 acggtcagtc aacccttcat cttcgtgaaa cgtcaaaatt attttcaggc acacctcaag
     5761 aacaactcgt tttgatgagc catggtgatg ctgttactga aattccagaa ggtttccacc
     5821 ttgttggaga ctcaaatgac tgtccctatg cagctattga aaatactgag aaaaaccttt
     5881 acggtattca gttccaccca gaagtgagac actctgttta tggaaatgac attcttaaaa
     5941 actttgctat atcaatttgt ggcgcgcgtg gtgattggtc aatggataat tttattgaca
     6001 tggaaattgc taaaattcgt gaaactgtag gcgatcgtaa agttcttcta ggtctttctg
     6061 gtggagttga ttcttcagtt gttggtgttc tacttcaaaa agctatcggt gaccaattaa
     6121 cttgtatttt cgttgatcac ggtcttcttc gtaaagacga gggcgatcaa gttatgggaa
     6181 tgcttggggg caaatttggc ctaaatatta tccgtgtgga tgcttcaaaa cgtttcttag
     6241 accttcttgc agacgttgaa gatcctgaga aaaaacgtaa aattattggt aatgaatttg
     6301 tctatgtttt tgatgatgaa gccagcaaat taaaaggtgt tgacttcctt gcccaaggaa
     6361 cactttatac tgatatcatt gagtcaggaa cagaaactgc tcaaaccatc aaatcacatc
     6421 acaatgtggg tggtctcccc gaagacatgc agtttgaatt gattgagccc ttaaacactc
     6481 ttttcaaaga tgaagttcga gcgcttggaa tcgctcttgg aatgcctgaa gaaattgttt
     6541 ggcgccaacc atttccaggt cctggacttg ctatccgtgt catgggagca attactgaag
     6601 aaaaacttga aaccgttcgc gaatcagacg ctatccttcg tgaagaaatt gctaaggctg
     6661 gacttgatcg tgacgtgtgg caatacttta cagttaacac aggtgtccgt tctgtaggcg
     6721 tcatgggaga tggtcgtact tatgattata ccatcgccat tcgtgctatt acgtctattg
     6781 atggtatgac agctgacttt gctcaacttc cttgggatgt cttgaaaaaa atctcaacac
     6841 gtatcgtaaa tgaagttgac cacgttaacc gtatcgtcta cgacatcaca agtaaaccac
     6901 ccgcaacagt tgaatgggaa taatcgcaaa attttctaaa atggcttgat tacaagctat
     6961 atagagaata aaaaatggct actggtactc aaatggtact ggaagctaaa aagaataaac
     7021 taaataatag ttccaagtgg tttacatttg gacaaaaaat
//
LOCUS       DATUYR010000068         7001 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.068, whole genome shotgun sequence.
ACCESSION   DATUYR010000068 DATUYR010000000
VERSION     DATUYR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.068"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(83..1342)
                     /locus_tag="VCI33_001456"
     CDS             complement(83..1342)
                     /locus_tag="VCI33_001456"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP2832375.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            complement(1516..2712)
                     /locus_tag="VCI33_001457"
     CDS             complement(1516..2712)
                     /locus_tag="VCI33_001457"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEP2832376.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTKAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            complement(3248..5626)
                     /locus_tag="VCI33_001458"
     CDS             complement(3248..5626)
                     /locus_tag="VCI33_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEP2832377.1"
                     /translation="MKTKKVIILVGLLLSSQLTLISCQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGMAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQQAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAYVADQYDPAKKAEKPAETPHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPEPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTSFPSNEKDPNKM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKRLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPNLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            5830..6771
                     /locus_tag="VCI33_001459"
     CDS             5830..6771
                     /locus_tag="VCI33_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEP2832378.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFTENSQEK
                     RAIAIDLTDQGHLIVQFENGDFQTLRSGEISLSSWEN"
     gene            complement(6746..6949)
                     /locus_tag="VCI33_001460"
     CDS             complement(6746..6949)
                     /locus_tag="VCI33_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEP2832379.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
BASE COUNT         2074 a         1495 c         1251 g         2181 t
ORIGIN      
        1 ccataaaatg caacaaagta tgcacctaag tattattagc ctaagcacat gtaatataaa
       61 aaagagaagc gttagcttct ctttagtctt cgattaattg aatatctgcc cctagtgctg
      121 ttaattttgc aatgatacta gcatagccac gtaagataaa ttcaatatta gtaatttctg
      181 tcttgccttc agctattaga ccagcagtca ctagagccgc tccggcacgt aaatcagtgg
      241 ctttgacctg agctcctgtc aggcgagacg gcccttgata aacaatctga ccaccaataa
      301 ctgagatatc tgctcccatg cgcatcaact ctggcacatg gttaatgcgc ttttcatata
      361 tagtgtcaat aatcgttcca cgaccatctg cttttaacaa caagggcgtt aatggctgtt
      421 gcaaatcagt ggcaaaacca gggtaaggag aggttttaat tgtaatagcc ttgagggatt
      481 cttgcttttc aacaaagata gcatcctctt ctaccgtcat acggacaccc atttcttcta
      541 acttagcaat gaaactctct aaatgctcat aaagaacatt agtgatctta acaccttttc
      601 caatagctgc cgccaaagcg atataagtcc ctgcttcaat acgatcagga ataacttggt
      661 gtctagtacc atgcaatttc tgaacacctt gtatcgtaat aatatctgta cctgctccac
      721 gaatatgcgc acccatgtta ttcaaaagag tagccacatc aataatctct ggctcacgtg
      781 cagcgttttc aataaccgtt ttaccttgag cttttgttgc cgcaaccata gtattaattg
      841 tagcaccgac actaacggtg tccatataga tatgagcgcc atgaattttt tgaccatttg
      901 tcgataaatt catattttca ccctcataag acacttcaac tcccatagcc tcgaaggcct
      961 tcaaatgcaa gtcaattggt cttggtccta aatcgcaacc accaggtaag ccaacaactg
     1021 cttgtccaaa acgacctaaa agactaccgt agaaataata ggaagctcga agactattaa
     1081 tctttccgta aggcatcggg atgtcttgca cccctctagg gtcaatttct aaagtatcac
     1141 catgatagtt tactgttgct cccataagct ccatgatttc aatcagacta tctacatcac
     1201 taatagcagg tacaccatca agaatcacaa tatcatcggc taagatgatt gctggaataa
     1261 gcgcaacaac actgtttttt gcccctgaaa ctgccacttc tccagataag gcttttccgc
     1321 cattaataat aattttacgc atacattcaa aactttctta tactagaatt caaccttttc
     1381 attttatcat aaatcatgaa gcctgacaat tattgacgtc ttaattttta atcatccctt
     1441 ttaaaggctt atttaataac aaaaaagcag agtaagattt tttcactcac tctacttttt
     1501 taataaagtt gtcgtttata atactgtttt tacagcttct actaaagcat caaccttatt
     1561 taaacgctcc catggcaaat caatatctgt tctccccatg tgaccatagg cagctgtttg
     1621 tttgtaaatc gggcgtttta aatcaagcat ttgaataatg cctgctggcc gtaaatcaaa
     1681 gacttgacga acagcagctt ctaaaacagc ttcaggaacc gttgaggttc caaatgtatc
     1741 cacgcgaaca gacacaggct gagccacccc aatcgcataa gctagttgga cttcagcttt
     1801 cgtcgcaaga cctgctgcca caaggttttt agcaatataa cgagcagcat aagaagccga
     1861 acggtccact tttgtcgcat cttttcctga aaaagcaccg ccaccatgac gtgaatagcc
     1921 tccataggta tccacaataa tcttacgtcc ggtcaatcca gagtctcctt gcggacctcc
     1981 aataacaaaa cgtcctgttg gattaatgaa gaactttgtg tcatcatcta aataatctgc
     2041 tggaattaca gccttgatca ctttttcaat cacatcctga cgaatttggt cattagtagc
     2101 ttctggatca tgctgagtag aaataacgac tgtatccaca cgaacaggtt tatcgtgttc
     2161 atcgtactca actgtcactt gagatttagc atctggacgt agataactaa tctcacctga
     2221 cttacgtaat tccgccaagc gacgcactaa ttggtgagat aaggaaattg gtaaaggcat
     2281 caattctggt gtttcattga tggcaaaacc aaacatgagc ccttggtcac ccgctccaat
     2341 atggctaagg tcatcagtat caccttctct gctttcgaga gcttcattaa ccccctgagc
     2401 gatatctcca gattgttcta caagtgatgg atgtactccc actgattctg ctgaaaagcc
     2461 atattctgct tctgtataac caatttctgc aatggtgtcg cggaccacac ggttaatatc
     2521 aatgtatgct tttgttgaga tttctccaaa aacatgaaca gaacctgtgt aaacacaagt
     2581 ttctgcagcc acatgggctt ctggatcttc tgctaaaatg gcatctaaaa tagcatctga
     2641 aatttggtct gcaatcttat ctggatgccc ttcagatacg gactcagacg tgaaaagttt
     2701 acgttctgac ataaaaatgt cccccctaat atgttgattc ttcctttttc agtcacattg
     2761 accacaaagc aagaagttgc ctaccccttt cttcctagct gattgagcaa gtcaagtggc
     2821 caaaaccaac ttagctaatc agttacggtt ttactagtaa ggcaactagc tagtccacct
     2881 agtggctagc gtccattaac cagacaaaga atgtctgaca atagcgttga gttacaaaga
     2941 atccggtttc cttgttgaga ggatgtcacc acaaaataag ccttagacga agtaatttag
     3001 ctaaagagag aacgactgtt ccccaaatga agtcaacaac gtctcaaaac atttcgttca
     3061 acagaccgtc ttatcgggac tcaataacaa tctattataa catgttttag ccatttaggt
     3121 gccattcttt tcactcatta tcctatttat ttcaaaaaaa cctcaaggtt attcttgagg
     3181 ttttcttgat agtcaacaat ggtgtcatta agacgattca gttggatcgg gttattctta
     3241 gattagttta gtcagcatgg ttttgctcat ctttagcttc attttcatcc tcatgttcat
     3301 ggtcgtgatc atctttgtct ctgtgctcgt gagcatgacc ttcttcatct tcataggcat
     3361 gattatggtt gtagtcatgt gattccgatg cttcttgagc actttctttc gccttagtag
     3421 ccatagctgc tttttcttca gctgttccat tacctaccat agaaccttcg taaaccgctg
     3481 ggaagttttt cgcaatggct tctggaatat gttgctcgtt caatttaagg tctgccaatg
     3541 ggatattgtt atgactaata tcaatgttag taactgtctt attgggttta gataggttga
     3601 catttgttaa ttggttttta gacacgctta aaatgtcaag tgctgtaaaa ttattaacac
     3661 cttctagaga agtcaactgg ttgcctgtca catccaagaa agtaagtgac ccttgcttgt
     3721 ctttaagcac aagcgattta atttggttac cttctgcttc tactctgaca atgacgctac
     3781 ttgcttcaat accttcaaga gattgcaatt gcgcacggtt aatagatagg ctagatagat
     3841 taggattatt tttcaagaaa tccaaatgag aaaccttggt atcattgacc attaacgttt
     3901 ctaagttggg tgcttgaagt gttgctaagt caacatcagc atttcttgat aaatcaacaa
     3961 ccgtcaatga ttctttatga gaaacagggc ttaaatctgt aatctgatta ttatcaatgt
     4021 gcaattcttg caattgatgt aacgatgcca gtgggcttaa atcagaaatc ttattattac
     4081 tcaatacgag gaatttgaga tttggtaatt gaccaagcgg cctaatatct tcaataccat
     4141 tatcagcagc cgctactaga gttaagtttt tatatttgct caagaaacta atatctttga
     4201 gattgttttg tgaaatatca atgccttcta actgtggcat attatccaaa aatctataat
     4261 cagtcacccc tgtttttgtc attagtaacc gtttcaattt tttaaattgc aaaacaggtg
     4321 agatatcttt gattggtgta aagccaattc ctaaagtttc taagttgggt aacagtgaaa
     4381 gtcctttacg ttgcaaagga tctttacggc tgcccaagtc aagtttgata accgttgcta
     4441 accattcttt catcttattc ggatcttttt catttgatgg gaatgaggtt ggagcatcgt
     4501 gagtgcgaac gatatccaaa atcacttctt catcaaaccc taaggcacgc aaggtatcca
     4561 ttccaacctt atgtttttcc aattcacggg catgctcaat agcatgtggt tctggaatgt
     4621 cttttccgat ttcaatatca gataacatca atacgtgtgc gtggtcatgg tgagggtatt
     4681 ccaaaccaag tttaccgtct tgtgtttcca cacgtttaat agttgatgga tcaatcccca
     4741 atttttctgc caaataagct aatttttctt ggtattcctt ttcacgttca gatagctctg
     4801 gtgtgtgagg ggtttctgct ggcttttctg cttttttagc gggatcgtat tgatctgcca
     4861 catatgccca gccaccctga cgaaggtccg caaaagaaat aggatgtaag tgaccatcgt
     4921 ggtccactaa aatactgtct tttgttaccc caacaatacc ttgaccgtta aattgaaaac
     4981 catctgaggt tgggaaatgc aagcctggaa taccattagc tgtagctgtt gaaacagggc
     5041 tactggtttg tggcaagcga gtagcaacct gtttagcttg tgcctgtgtc tgacctgata
     5101 agcttgactt caaaatataa tggaagtgat catcgtggcg aaccgtgtag cctaaagcat
     5161 cttcagccac aatatccgct gggttaaatt cataatggtg atgaggatct gctactttag
     5221 aagtcccttg actagctgct tgctgagcaa cagctggctt agctaaactt gctccttttg
     5281 gaataaggta ttcaaatgga cttcccttta aatcggcata aaaaataaaa tgcgaatgac
     5341 catcatggtc aacaacgatt ccctgatctg tttttgataa gatttttgag tctttggtta
     5401 aaataaaccc atcatctgta ggaaaatcga cacccgccat acctttgtga gtcttgtttg
     5461 tctttttgct ttttttaatc ggtttaattt tgtttgacgt cattccctta cgtgattgtt
     5521 tcgttttaat gggatatgta ccattacctc gtgattgaca agatatcaaa gtcaactgag
     5581 atgataacaa tagaccaact aaaataataa cttttttcgt tttcattcaa ccctcctttt
     5641 tatttaactg gttaagtata tcacatcact tgtttttcct caagctcttt ttaggataac
     5701 tattagttgt tgacgagttg gagctctgaa gcttcaacaa acaatagttt ctgttgttag
     5761 tagcattaca caaaaaaact aacttttctt ttcctttgaa aataagatct tttataatat
     5821 actatacata tgaaaacatc agaaaaaatt tatcaattac tgtcacaaac cgatgacttc
     5881 gtcagtgggg aatatctagc agatcaactc agtatctctc gtacatccgt ttggaaatcc
     5941 atcaaaagtc tcgaaaatca ggggattcaa attgattctt taaaacacaa gggctaccgc
     6001 atggtacaag gtgatatttt attacccaaa accattagcc aagggcttgg gatgccagtc
     6061 acctataccc ctcacagcca atctacccaa ttagatgcca aacaaggaat cgaagctcat
     6121 aacagcgctc caaggcttta cctagctcct agccaagaag ctgccaaagg acgcctcgat
     6181 cggcaatttt tttcagctag caccggtggt atttacatgt ccatgtacct aaaacccaat
     6241 gtcccttatg ctgatatgcc tccttatacg atgatggttg cttccagtat tgtcaaagct
     6301 atctcgcgat taacaggcat tgatacagaa atcaaatggg taaacgacat ctatctaggt
     6361 aaccataaag ttgcagggat tctgacagaa gccattactt ctgttgaaac tggtttaatc
     6421 accgatgtca ttattggggt tggtctcaac ttttttgtca ccgacttccc agaagcgatt
     6481 gctcaaaagg caggttctct ctttaccgaa aaaccgacta tcacgcgcaa tgaccttatc
     6541 attgacatct ggaaattatt cttatctatt cctgtcaagg accatgtcaa agtttacaaa
     6601 gaaaaatcac ttgttcttaa taaacaagtg acctttacag aaaatagtca agaaaaacgt
     6661 gctattgcga ttgatctcac agatcaagga cacctcattg tacagtttga aaatggtgac
     6721 ttccaaacct tgcgaagcgg tgaaatcagt ctttcttctt gggagaatta gccgttttta
     6781 aaatggtttt agtcaattgg ttaatggtat gcactcttct taaatcccta aagagcaaca
     6841 agccccaaaa taaggcgaag agataatttc cagagagcaa caggtcatta aaaaaatagg
     6901 tttcaaaagc acatacaaag gccataaaaa gaaattgtcg aatagtcata gctttattat
     6961 aacagaaaaa aatgattcta tgaagaatcc acacagattg a
//
LOCUS       DATUYR010000069         6767 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.069, whole genome shotgun sequence.
ACCESSION   DATUYR010000069 DATUYR010000000
VERSION     DATUYR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.069"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            64..837
                     /gene="cfa"
                     /locus_tag="VCI33_001461"
     CDS             64..837
                     /gene="cfa"
                     /locus_tag="VCI33_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEP2832380.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKSFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
     gene            complement(1207..2442)
                     /locus_tag="VCI33_001462"
     CDS             complement(1207..2442)
                     /locus_tag="VCI33_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEP2832381.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLLLSAAKLLAGYLSNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKRIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMILEEEIFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLVDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQHTSIWRRHESWSLLFHQ
                     ITPIAKRQLHHTHYRIVKM"
     gene            complement(2562..3884)
                     /locus_tag="VCI33_001463"
     CDS             complement(2562..3884)
                     /locus_tag="VCI33_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEP2832382.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNESVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(4012..4098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4413..6731
                     /gene="pcrA"
                     /locus_tag="VCI33_001464"
     CDS             4413..6731
                     /gene="pcrA"
                     /locus_tag="VCI33_001464"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEP2832383.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALSLNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         2265 a         1271 c         1332 g         1899 t
ORIGIN      
        1 ttataaatta atttaacttc acttaactat ttaaataggt ttattatatt caggaggaat
       61 aatatgaaac ccaaacacct tctctgtctt tcaacagttg ttgctggatt agctttattt
      121 tcaactatga cgcactcagt tttagctgat gatgcttcaa atcccgatac tattttaatg
      181 aataacaacc aagcgaatct tcaaagagac gctctagtgc aaaagcttga tgagggacat
      241 caacaattag aagctattaa acatgaagct aaaggtactg atattgaggc tactgttaac
      301 aaagctatcg atgctgttga tcacatgaag agttctatac gcttcaatac tgaaacgatc
      361 tatgatttta gttcaattgg ggcaagagta gaagcattat cagatgctat caaagcaatc
      421 gtattttcca caacccaatt aactcacaaa gtagaaaaag cgcatactga tatggggttt
      481 gctattacta aattagtcat tcgtattata gatccatttg catctgttga cgctatcaaa
      541 gctcaagtac aagaaattaa agcacttgag gaaaaagtta tcaattatcc tgatttacag
      601 ccaacagacc gtgctactat ctatactaaa gctaaactaa ataaagctat ttggaacact
      661 cgtttagaaa gagataaaaa ggttttaggg atcaaatcat ttgacgttta taatagactt
      721 aacaaagcta tcacacatgc ggttggtgtc caactaaatc cgacaaccac tgttcaacaa
      781 gttgacgatg aagttattgc tgtgcaaaat gctttggaaa cagctcttaa gtcatagagt
      841 gtataagtaa tgtgttgtat aggtagatga ttatattagg atatagaata ggtaaagatt
      901 ttaaatatct taccattgta gagtttgagg tgatgttctc ataaacaatg agtcacctgt
      961 agcccttttt gcctttaaca tgatgtacca atttaaggta ttgttcaacc gtcactattg
     1021 aatcttgttt aacctcctaa actaataaga ttcaatatga aaacgtgtat tcttaaaaaa
     1081 atccctatga tctgtttaaa aacaaattgt agggattttt ttaactattt aacttattat
     1141 tttgggacta ggaatcgctt taggaatatc atgaagtata gttatgttat aagataagta
     1201 atgtttttac atttttacaa tacgatagtg tgtgtgatgt aattgtctct tagcgatagg
     1261 ggtaatttgg tggaagagta aggaccaaga ttcatgacgc ctccaaatac tggtatgttg
     1321 attgccattt aattggtaag ttaccaacat cgttttttga ctaatggatt caatctgaaa
     1381 atggttgaag ttacttggat aataaatttc ttggttcaaa aattgttcat agttaactgt
     1441 ttggccattc gcatcaacca gttggaaaga cttagcaatg tagtggtcat agtcaggaac
     1501 cttactcaaa attaattttt cgtagcggta gagctttttg gcgacattat caaaaatctc
     1561 ttcttcgaga atcatggcag gttcaacgtg aatatcaatg tcataaatag aaaattggtc
     1621 actcaataac tgctccactt tctctgtaat agagtgactt tcatagactg aaagatcagg
     1681 attcatttca agtacaatat caagatagac attgctacca taggtcctag ctcgttgaga
     1741 cttaacggcg acaattttag ggatttctaa aatggctttt tcgtattttt tcaaatgacg
     1801 gctatcaaat ccatcagata aactaaacga actttccata aagatatcaa aagctgtttt
     1861 aagaataaag aacgtaatga tcatagcagc tatatgatcg ataactggta aatgcaaaga
     1921 agctgctata atagcaattg atgttccgat agaagtaaca gcatcagcta gattatcctt
     1981 agaagcagcg actaaggcac tggattttac acgtttggaa agacgtttgt taaagacata
     2041 gacccctaac ataacaaaag ctgaaacgat accgacaata gccccaagag ggtcaatatc
     2101 aacttgctga ccactaaaga tacgtttaat tgtgtgaatc agtacttgga aacctacaag
     2161 aaacataata aaagaagtga caaggctgga taaatcttca attttccaat gaccaaattt
     2221 atgattggca tcggctggtt ggctagctaa gtgaagacca ataagcaggg ctacatttcc
     2281 cacaatatcc gataaattgt taaatccatc agcaataagg gaacttgcat ttgacaaata
     2341 accagctaat aatttggcag cactaagcaa caagtagaca ataatactaa cgattgggcc
     2401 ctttctggct agttttaaat ttgcaatcgg atcctgtgtc ataataactc ctttgatttc
     2461 aactttatat tataccataa aaaggggctt aagaaaaaag cctagcttct tgaacttggc
     2521 agaagaagac taggtataac tgggtagtac taacgtcatg tttacttaat aaagtaatgc
     2581 ttggtttcta aaataacaac tggcgagaga gctaggaggg caatcaggtt aggaagtgcc
     2641 attaaaccat tgacaatatc agccaaaacc caaatcagtt ctaacttaag gaagcctcct
     2701 aggcctacca ttaagataaa gacaatacgg aagtaggtga gatgtgtaat gccaaagagg
     2761 aattcaaagc agcgttctcc ataataactc catcctaaaa tcgttgtaaa ggcgaagaga
     2821 actagggaaa aggttaggcc aaaagtccct aaatttccaa acacagtggc aaaggcagat
     2881 tgggtcagag gagccccttc gagctgccca gtccattggc ctgttactaa aatagatagg
     2941 ccggttaggg tacagataat aatcgtatca ataaaagttc ctgtcatgga gatcaggccc
     3001 tgctccacgg attcattggt tttagctgct gctgcagcaa taggtgcaga tcctagtcca
     3061 gactcattgg agaagacacc gcgtgcaatt cctttttgaa tggcatcctt catgagactt
     3121 ccagcaaagc ctccaatagc agctgtcgga gtgaaggctg attgaaagac taaacgaata
     3181 acaggaagta gctgctggta gtgactaaaa ataactgcta aactagatag aatataaaaa
     3241 atagccataa aaggaacgac tttttcagcc actttggaaa tggaatgaat gccaccaaaa
     3301 ataatggcag ccacaaagat agctaaaaca atactgacca tttgtgggga taagccaaag
     3361 ctatgaccta aagacgaggt gatcgagttc acttgagcaa aggttcctat tccaaatagg
     3421 gcaactagga tccctgaacc cgcaaaaaga atggctaagg gtttccattt tgtccccatg
     3481 ccattaacaa tatagtacat aggaccacca gaaatatgac cgttggcatc ctttgtacgg
     3541 tatttaatcg ctagtacccc ttcagcgtac ttagtggcca ttccaaaaaa agcggcaacc
     3601 cacatccaaa agagcgctcc aggaccacca gacttgatag cagtggcaac cccaacaatg
     3661 ttaccagttc cgacagtagc ggcaagggca gttgcaagag cagcaaagga tgaaatatct
     3721 ccatgtcctt cgtcatctga aaaaatgagt ttaaaggctc ttggtagttt taagatttga
     3781 attaatccta aacgactggt aaggtaaatc cccgtcccaa ccaataaaat taagaggggc
     3841 ggtccccaaa caaggttatc aattaattta acgagtgcta tcatctttct ctcctaaaag
     3901 tcatcgaaga cgccaaaaga aaaaagctat ctctttcttt gagacaactt ctgaagcaat
     3961 gataaaacag caaacacaaa aaagactaag actgttctta atggtgtttg tattttacct
     4021 tctgtccttt tgcctgagag attgagcagt atgccttgcc ccttcggcgc ctaataaggt
     4081 ctctccagaa gtccgtccat caaacagtcc cgttcaaaaa ctgaacacct gagagtataa
     4141 ctccttcggc gaaaaaaatt cttctcctgc atgacatcat tcggtttatt aaattaggac
     4201 tatcatacct caaaaaacga atgaagtcaa ccaaaatagc aaaaaaataa ggaatgcttt
     4261 aagaaatcgc tattattttt aaatatctga tcctaaacta gaaaaaagga atctctttac
     4321 ttttggggtc ttctttgcta tttttcttgg aaacatgata taattttaag gttgataaaa
     4381 gagatgtcac tgaacgagat taaggagaaa gcatgaatcc tttattaaat ggaatgaatg
     4441 atcggcaggc acaagctgtt caaacaactg aaggcccctt attgattatg gcgggggctg
     4501 gttctggtaa aacaagggta ctgacccatc gtattgccta tttaattgat gaaaaatttg
     4561 tcaatccttg gaatatttta gctattacct ttactaataa ggctgcgcgt gaaatgaaag
     4621 aaagagcctt gtccttaaat ccagctacta aagatacctt aattgctacc tttcactcca
     4681 tgtgtgtacg gattctgcgt cgtgaggctg atcacattgg ttacaaccga aacttcacaa
     4741 tcgtggatcc aggtgagcaa cgaacgttga tgaagcgcat tttaaaacaa ttaaatatag
     4801 accctaaaaa gtggaatgaa cgttctattt taggaactat ttcaaacgct aaaaatgatt
     4861 tgcttgatga aaagggatac gaggcacagg ctgctgatat gtatagtcaa atcgtagcga
     4921 ggtgttacaa agcttatcag gaagaattac gacgtagcga agcacttgac tttgatgatt
     4981 taatcatgat gacgctacgc ctgtttgacg ctaatcctga cgtgttagct tattaccagc
     5041 agcgttacca atacattcat gtggatgagt accaagatac caaccatgct caatatcagc
     5101 taattaaatt gctagcttct cgctttaaaa atatttgcgt tgttggagac gctgatcagt
     5161 ctatctatgg ttggcgggga gctgacatgc aaaatattct tgattttgaa aaagattacc
     5221 cagatgctaa ggtagtgctt ttagaagaaa actatcgctc taccaagaaa attctgcaag
     5281 cagctaacga cgtgattaac aataaccgta atcgccgtcc taagaaactt tggacacaaa
     5341 atgcggatgg tgaacaactg gtttattacc gggcaaatga tgagcgagat gaggcagtat
     5401 ttgtggcctc aaccattagc aatatgagtc aagagcttgg aaaaaacttc aaagatttcg
     5461 ctgttttata ccgtaccaat gcccaatctc ggaccattga agaagcactc ttaaaatcca
     5521 atattcctta caccatggtt ggaggcacca agttttacag ccgtaaagaa attcgggatt
     5581 tgattgccta tttaaccatt gttgctaacc ctgctgataa tatttctttt gagcggattg
     5641 tgaatgagcc taaacgaggt gttggtccag gaacgcttga taaactgcgt cagtttgcct
     5701 atgagagtga ccagtcttta ttagaagcag cttcaaatct attgatgtcc cccttgaaag
     5761 gtaaagcagc acaagctatc atggacttgg ccaatattct cggtcagcta cgtcaagacc
     5821 tagatcagat gagtatcacc gacttagcag aagctttgtt agaaaaaaca ggttatttag
     5881 atagcttacg cctgcaaaat acattggaaa gccaagcccg tatcgaaaat atcgaagaat
     5941 tcttatctgt taccaaaaat tttgacgagt caagtgcctc tcaagaagaa gacgaaaccg
     6001 gtgttgatcg cttgggacgt tttttaaacg atttagcact gattgctgat acagatgatt
     6061 cccaagcaga agctgctgaa gtaaccttaa tgaccctaca tgctgccaaa ggcttggagt
     6121 tccctgttgt tttcttaatt ggtatggaag aaggtgtctt ccccttatca cgtgcctctg
     6181 aagatccaga tgaattggaa gaagaaagac gtttagctta tgtcggaatc acaagagctg
     6241 aagaagttct ctttatgacc aatgccaaca ccagaaccct ctttggtaaa agtagttata
     6301 accgtccaac acgcttcttg aaagaaattt cagaggaact actctcttat aaaggattgg
     6361 caagaccagc acaatcttca tttggtgtgc gtttttctac tgaaacacac acacagtttg
     6421 gacaagggat gagcctatca gaagccttac aagctcgaaa ggcccaggct caagtaagac
     6481 aatcagcaca gccaatgcag gcacatacga taccatcagc ttcaactagc tcagttttac
     6541 cgtttggttc taatagctct gtagaagagg tcacttggca gattggcgat atcgctcacc
     6601 ataaaaaatg gggagacggg actgtgcttg aggtttcagg ctccggaaaa acgatggaat
     6661 tgaaaatcaa atttccagaa gttggtctaa agaaattact agcaagtgtt gctcctattg
     6721 agaaaaaata agaaaaaaat gagcttccta ctatataagt gagtagg
//
LOCUS       DATUYR010000070         6691 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.070, whole genome shotgun sequence.
ACCESSION   DATUYR010000070 DATUYR010000000
VERSION     DATUYR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.070"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            355..1071
                     /locus_tag="VCI33_001465"
     CDS             355..1071
                     /locus_tag="VCI33_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP2832384.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            1274..2116
                     /locus_tag="VCI33_001466"
     CDS             1274..2116
                     /locus_tag="VCI33_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP2832385.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYRVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            2443..3288
                     /locus_tag="VCI33_001467"
     CDS             2443..3288
                     /locus_tag="VCI33_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP2832386.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            3538..4602
                     /locus_tag="VCI33_001468"
     CDS             3538..4602
                     /locus_tag="VCI33_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEP2832387.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSADALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            4603..5295
                     /locus_tag="VCI33_001469"
     CDS             4603..5295
                     /locus_tag="VCI33_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832388.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(5349..6683)
                     /gene="brnQ"
                     /locus_tag="VCI33_001470"
     CDS             complement(5349..6683)
                     /gene="brnQ"
                     /locus_tag="VCI33_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEP2832389.1"
                     /translation="MLFALFFGAANLIYPAFLGIYSGHQILWSIIGFCLTGVSLPLLG
                     VIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGATSFSVGIAPILG
                     DNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISILVIASFVHPAGNY
                     GDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEATKQFGAKTDKEVT
                     KITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPVNGGQILSHASRF
                     YLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWATIFTLLSAFFYF
                     GGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPVVYRTTIGLTFIPAIFDALL
                     TLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLILSKTKKS"
BASE COUNT         2293 a         1088 c         1361 g         1949 t
ORIGIN      
        1 acaggctaac gtttttacaa gattatatca gtaatcattt tgacaattgt agtaatggaa
       61 tgttgccaaa atttagaatg ttgtgtgtta tgaatgacaa gattttaaag gaatactaat
      121 taaacgggag gtcgtgaacc ggaagctgtt gtcactcaag cgccaaaatc tgtctgtaac
      181 ataatattga tgtatgggaa cgcaattatt tggtttttat tgtgatgctt aaaacgattt
      241 tatatttcta ttttaccaaa gcttttcaaa gtgctataat aagggagtta gagtcaatat
      301 tagtaacgtt aatgttgaca atattcaata aaatactatt aaaaggagac aaaaatggga
      361 cgtaaatggg caaatattgt tgctaaaaaa actgctaaag atggagcaac atcaaaggtc
      421 tatgccaaat ttggtgttga aatttatgtg gctgccaaac aaggcgagcc agatccagag
      481 ttaaatacag ctcttaaatt tgtcattgat cgtgcaaagc aagcacaagt gccaaagcat
      541 gttattgata aagcgattga taaagctaaa ggaaacacag atgaaacttt cgtagaggga
      601 cgctatgaag gttttggtcc aaatggttca atgattattg tggatacttt gacatcaaat
      661 gttaaccgta cagcggcaaa tgtacgtacg gcttacggta agaacggtgg caatatggga
      721 gcttcaggat ctgtatccta cttatttgat aaaaaagggg tcatcgtttt tgctggtgat
      781 gatgctgaca gtgtctttga acaattactt gaagcggatg tagacgtaga tgatgttgaa
      841 gcagaagagg gaacaataac agtttatacc gccccaacag atcttcataa aggtatccaa
      901 gcacttcgcg ataatggtgt agaagaattc caagttactg aacttgaaat gattcctcaa
      961 tcagaagtag tattggaagg tgatgacctt gaaacttttg aaaagcttat tgatgcactc
     1021 gaatcagatg atgatgttca aaaagtgtat cataatgtag cagattttta aattgacata
     1081 gaataataaa aagagttgaa aactatttca gctcttttta agtgatgctt ttataagtaa
     1141 gtaccaagta acatagtcgt tgttagaaga taatgaacaa tagcaaatca gtatcaactt
     1201 gataggaatc aattattagt gaactctaat taaaaatggt aaactagaca caagaacaaa
     1261 aaggagagat aggatgatta aaaaacaata tttaggaagg gcaacaattg ccctagcatc
     1321 aacccttgta ctagcagctt gtgggtcatc aaaaacagct gagtcaggaa atcaggggag
     1381 ttctaaagaa gtcttatttg caacggttgg aacaacagct cccttttctt atgaaaaagg
     1441 tggtcaacta actggctacg atatcgaggt ggctaaagca gtctttaaag gatcggatga
     1501 ttacagggtg tcctttaaaa aaacagaatg gtcttctatc tttacaggtt tggactcagg
     1561 aaaataccaa atgggtggta acaatatttc ctttaccaaa gagcgttcag ctaagtacct
     1621 tttttcttat ccaatcggat caacaccttc tgttttagtt gttccaaaag acagtgatat
     1681 caagagcttt gatgatatcc aagggcatac gacacaagtt gttcaaggga ctacaagtgt
     1741 tgcacagtta gaagacttta ataagaaaca cagtgacaac ccggttactc taaaattcac
     1801 caatgagaac attacccaaa tgttaaccaa tttgagtgaa ggcaaggcag atttcaagat
     1861 ttttgacgct ccaacggtta acgccattat taaaaatcaa gggttagata atctcaaaac
     1921 aattgaactc acctcaacag agcaaccatt tatttatttt atctttagtc aagatcaaga
     1981 gaaattgcaa agctttgtta acaagcgtat taaagagttg actgctgacg gaactcttag
     2041 caagcttgcc aaagagcacc ttggcggtga ttacgtgcca tcagataaag agttgaaact
     2101 tcctacagct aactaaacca tctattaacc agttattcat ctagaagact ggtttttctg
     2161 atcggaaaca atattatagt ttatgaggtt atgttataaa aattgcaaat agccttaatc
     2221 aaaatgagat gttaaagtgc tttttgagaa gtgaatattg taagaagtat gctttgggtt
     2281 ttagtcctca aataccaagt tttttgttga ctaaacaaat aaggatggtt agcatagcat
     2341 atagtttaaa actatggtat atgctagcca ttatcaattt gaaaactata tctaattaag
     2401 atacaataat gatcgtaaac aaaatgaatc gaggataacg ctatgtcttt taaaaaatgg
     2461 attggaatag tcggaatcgc tttagcatca actgtattgg ttgcttgttc tgaaaaacaa
     2521 gatgataaaa acactctaac tattggagtg atgacaaaaa cagaatctga tcaagcgaga
     2581 tgggataaag ttgaagaatt attgaaaaaa gacaatatca ctttgaagta caaagaattt
     2641 acagattact cgcaacctaa taaggcagtt gctaatggtg aagtcgatat taatgccttc
     2701 caacactata atttcttaaa taactggaat aaggaaaata aggaacattt ggtggccatt
     2761 gctgatacct atatcagccc aattaacctt ttctctggaa ccagtcaaga tgggaaggct
     2821 aaatacaaat cagtagctga tctgccaaat ggtgctcaaa ttgcagtacc aaatgatgcg
     2881 accaatgaaa gccgtgccct ctatgtgctg caatcagctg gcctcattaa attgaatgtg
     2941 tctggtgatc aattagcaac cattgccaat atctcagaga acaaaaagaa attagatatc
     3001 aaggaattag atgctagcca aacggcacgt gctttagtat ctgctgatgc agctgttgtc
     3061 aataatagtt acgctgttcc tgcaaaaatt gactacaaaa cctctctttt caaagaaaaa
     3121 gcagatgaca attcaaaaca atggattaac attattgcag gtcaaaaaga ttgggaaaaa
     3181 tcagagaagg ctgatgctat taaaaaactc atcaaggcct accaaactga tgaggtgaag
     3241 aaagtcgtgg aaaaaacttc aaatggtata gatgtatccg tatggtaaat tttagccatt
     3301 gttataacaa gagtgtggtc gtgtttaaaa cacatgcttt cttgcatgct tgaggaagag
     3361 tttacgacta atttaccttt agaaagctat ttaagacctt ttccctttta ggatttagaa
     3421 accttgggaa gactattgca tgttatctta atagtcatga ttatgcaggc gatgaaaaca
     3481 ttgctatcgt agattactgt cgccatatcg tactaataga ggagttatta aaatcttatg
     3541 aatgaagcaa taattcaatt agatcatatt gatattacct tccgtcaaaa aaaacgggtt
     3601 atcgaagctg ttaaagatgt cacggttcat atcaatcaag gagatattta tggcattgtt
     3661 gggtattcag gtgccgggaa atcaactctt gtgcgtgtga ttaacctgct acaggcacca
     3721 acaaacggga aaatcactgt tgatggggat gtgacctttg accaggggaa aattcaattg
     3781 tcagctgatg cccttcgcca aaaacgacgt gatattggta tgatcttcca acatttcaat
     3841 ttgatggccc aaaaaacagc caaagaaaat gtagcatttg cgctccgtca ttcgtcgtta
     3901 agtaagactg agaaagaaca taaagttatc gagctgctag aacttgtagg tttatcagag
     3961 cgagcagata actaccctgc ccagttatcg ggtgggcaaa aacaaagggt ggctattgcg
     4021 cgtgctctag ctaacgatcc aaaaattttg atttcagatg aggcaacatc agcactagat
     4081 ccaaaaacca ctaaacaaat tttggcgctc ttgcaagaat taaaccgcaa acttggcctt
     4141 accattgtta tgattactca tgaaatgcaa attgttaagg atatttgtaa tcgtgttgct
     4201 gttatgcaaa atggtgtttt gattgaagaa ggttctgtcc ttgatatctt ttcaaatcca
     4261 aaagaagcct tgacacagga atttatcaca acagctacag ggattgatga ggctttagaa
     4321 aaaattaacc aacaagacat tgttaaacat ttgcctgcaa atgcccttct agctcagttg
     4381 aaatatgcag gaacttctac ggatgagcct ctacttaata gcatttaccg tcaatttgaa
     4441 gtgacagcta atattttgta tggcaatatt gagattttag atcatattcc agttggtgac
     4501 atgattgttg tcttggaagg gcaagctgaa aatattcttg cggctgagaa agccttgcat
     4561 gaagctggtg ttgatgttag tattttaaag agaggagcct agatgtcaca gttaattcaa
     4621 acttatttgc caaatgttta tgaactcgga tggtctggag atgctggttg gggcttagcg
     4681 atttggaata ctttatacat gaccattgtg ccttttattg ttgggggtgc tattggtctc
     4741 ttattaggct tattattagt tttaacagga ccggacggtg ttattgaaaa caaaacgatt
     4801 tgttgggtta ttgataaagt tacctctatt tttagagcca ttccttttgt tattttgatt
     4861 gctattttag ctagctttac ctatttgctt ttaagaacaa ctttaggagc aacagcggcc
     4921 ttggtacctt taacctttgc gacttttcct ttttatgccc gtcaggtcca agttgttttt
     4981 tcagaattag acaagggtgt tattgaagca gcgcaagctt caggtgctac tttctgggat
     5041 attgtcaaag tgtatctcag tgaaggttta ccagatctta ttcgtgtttc tacagtcacc
     5101 ttaatttctt tagttgggga aactgccatg gcaggagcta tcggtgctgg aggattagga
     5161 aatgtagcga tttcttatgg ttataatcgt tttaacaatg atgtgacttg ggtagcgact
     5221 attattattc ttttaattat ttttgctatc caatttattg gtgatagctt gactagaagg
     5281 tttagtcata aataaacgaa acaacaccct aagtttttac ttagggtgtt gttgtttaga
     5341 tgatgtcatt agcttttctt cgttttactt agtataagcc ctattaaaaa accaatagca
     5401 gcaaagataa tccatcccat tgagaattgc cctagtggaa cagttttttg gaaaaaagtt
     5461 acaacggctt ctggtaaatg aaataatcca gtcatttgtg atagtgttag gagtgcatca
     5521 aatatggcag gaataaaggt tagaccaatt gtagttcgat agacaacagg atcattattg
     5581 aagcattttt gtgccaaaac aaggaaaatt aaatcgactg ttaatgggta taaaaggaat
     5641 aaaacaggag ctgaccagtt gataataact gataaaccac caaaataaaa gaaagctgat
     5701 agtaaagtaa agatagttgc ccaagcaata tgagataaag caggcactaa tttatggaag
     5761 tattcagcac ttgacgtgat taagcctgtt gaagtggtta gacaggccag gaaaatcaca
     5821 acagctagaa atgcttgtcc gatgccacct aggtaaaaac gagaagcatg acttaagatt
     5881 tgaccgccat taactggatt accatgaagg gtaaagctgc catcactaaa aggaaataat
     5941 gattgtgatg ttgctccaat acgaccgaca aagatataga caagtgctaa tagcaaaatg
     6001 gcaatagccc cagaaataag tgttattttg gtcacttctt tgtccgtctt agcgccaaat
     6061 tgtttggtag cctcaatgac taaaatagca aaaacaagag aagctagtgc atccatagtg
     6121 ccataacctt gaattaatcc tgctataaaa ggaaaatctt taaaggcatt attaacaccg
     6181 accccagcgt taaaagcatc accgtaattt ccagcaggat ggacaaagga cgcgataacc
     6241 aaaatagaaa taacaactaa caacgttggc gttaaaaatt ttccgatatt ttcagctagc
     6301 ttactaggtt tgatagcaag gaagtaggac agaccaaaaa atagtatagc ataaatagct
     6361 ttattggttg tattatctcc taagatagga gcgataccga ctgaaaaaga agtggctcct
     6421 gttcttggaa tagcaaaaaa tggaccaata gataaataca aaatggaaga atagaagatt
     6481 gcataccatt tagagatggg gcgtgccaaa ctttcaacat cgcctgatcc agatttagca
     6541 acagcaatga caccgagtaa aggcaaggag acaccagtta aacaaaaacc aatgatagac
     6601 catagaatct gatgacctga gtagatgcct aaaaaagctg ggtaaatgag gtttgctgct
     6661 ccaaaaaata gtgcaaatag catgaatcca a
//
LOCUS       DATUYR010000071         6685 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.071, whole genome shotgun sequence.
ACCESSION   DATUYR010000071 DATUYR010000000
VERSION     DATUYR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.071"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            109..987
                     /gene="mvk"
                     /locus_tag="VCI33_001471"
     CDS             109..987
                     /gene="mvk"
                     /locus_tag="VCI33_001471"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEP2832390.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLVIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            969..1913
                     /gene="mvaD"
                     /locus_tag="VCI33_001472"
     CDS             969..1913
                     /gene="mvaD"
                     /locus_tag="VCI33_001472"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEP2832391.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISTIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            1906..2919
                     /locus_tag="VCI33_001473"
     CDS             1906..2919
                     /locus_tag="VCI33_001473"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEP2832392.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFSHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHSAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQNARDTLISKWQEADIALLYQERWEEN
                     DQP"
     gene            2906..3895
                     /locus_tag="VCI33_001474"
     CDS             2906..3895
                     /locus_tag="VCI33_001474"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEP2832393.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(4125..5402)
                     /locus_tag="VCI33_001475"
     CDS             complement(4125..5402)
                     /locus_tag="VCI33_001475"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEP2832394.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQTGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(5389..6564)
                     /locus_tag="VCI33_001476"
     CDS             complement(5389..6564)
                     /locus_tag="VCI33_001476"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEP2832395.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRSKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHRRIYHTNDKN"
BASE COUNT         2050 a         1309 c         1365 g         1961 t
ORIGIN      
        1 tttcagtgaa gaaatgattt taaaaagata tatcatcgca ttttctaatc atttagtgta
       61 aaatagtaag gtaaaagttt agaagaatta gtaatgaaaa gataaaaaat gaatgaaaac
      121 attggatatg gtaaggcaca cagtaagatc attttgatag gtgagcatgc tgttgtgtat
      181 ggctacccag ctattgcttt gcctttaaca gatattgagg tggtttgtca tatttttcca
      241 gctgataagc cattggtctt tgatttttat gatactctat caacagccat ttatgctgcc
      301 ttggattatt tgcagcgact acaagaacca attgcttatg agattgtttc acaagtgcca
      361 caaaagcgtg gtatggggtc ttcggcagcc gtttctattg cagctattag agccgttttt
      421 tcttattgtc aagaacccct ctcggatgat ttgttggaaa ttttagtcaa caaggcagaa
      481 attattgccc acaccaatcc tagtggttta gatgccaaga catgccttag tgaccatgcc
      541 attaagttta tccgaaacat tggctttgaa actatcgaaa tcgccttaaa tggttatctt
      601 gtcattgcag atacagggat tcacggtcat acacgtgagg ccgtcaataa ggtagcacag
      661 tttgaggaaa cgaatttgcc ctatctggct aaactaggag ctttgacaca agcccttgaa
      721 agagcgatta accaaaaaaa taaggtggct atcggtcagc tcatgacaca agcgcactct
      781 gctttaaagg ccattggcgt tagcatcagt aaagctgacc aacttgtgga ggctgcccta
      841 agagcaggtg ctttaggcgc taagatgaca ggcggtggct tgggtggctg tatgattgcc
      901 ctagcagata ccaaagacat ggccgaaaaa atcagtcaca gattaaaaga agaaggagcc
      961 gtaaacacgt ggatccaaat gttataaccg taacatccta tgctaacatt gctattatca
     1021 aatattgggg aaaagagaat caagctaaga tgattccttc gacctctagc atttctttga
     1081 ctttggaaaa catgttcacc acaaccagcg tttccttctt accagatact gcaaccagtg
     1141 atcagtttta cattaacggt gtcttgcaaa atgacgaaga acataccaaa atttctacta
     1201 tcattgacca atttcgccaa cctggtcagg cttttgtaaa gatggaaact caaaataata
     1261 tgccaacagc tgcaggttta tcatcaagtt ccagtggctt atcagccttg gttaaagcct
     1321 gtgatcaatt atttgacact cagctagatc agaaagcttt agctcaaaag gctaagttcg
     1381 cctcaggatc atcttctcgt tctttttttg gcccagttgc tgcttgggac aaagatagtg
     1441 gtgctattta caaggttgag actgacttga aaatggccat gattatgctg gtcttaaatg
     1501 ctgcgaaaaa gccaatttct agccgagagg gcatgaagtt atgccgcgat acctcaacta
     1561 catttgatga atgggtagaa caatcggcaa tcgattacca acatatgctt acctatctca
     1621 aaactaataa ttttgagaaa gttggtcagt taacagaagc taatgccttg gccatgcatg
     1681 ccacgacaaa gacagccaat cctccctttt cttacctaac aaaagagtct taccaggcta
     1741 tggaggctgt gaaagaactg cgtcaagaag gttttgcttg ttattttacc atggacgcag
     1801 gtccaaatgt gaaggtccta tgtttagaaa aagacttggc tcaactagca gaacgacttg
     1861 gcaagaacta ccgtattatc gtttcaaaaa caaaggattt accagatgtc taattattgt
     1921 gtgcaaacag gtgggaaact atacctcaca ggcgaatatg ctatcttaac accaggacaa
     1981 aaagccttaa ttcactttat tccactgatg atgacagcag aaattagccc agcagcccat
     2041 attcaattag cttcagatat gttttcccat aaagcgggca tgacacccga tgcctcttat
     2101 gcactgattc aagcaacggt taaaaccttt gctgattatc taggacagtc aattgaccaa
     2161 ctggagccat tttccctaat tataacggga aaaatggagc gcgatggcaa aaaatttggc
     2221 attggttcaa gtggtagtgt caccctctta accttaaagg ccttatcggc ctattatcag
     2281 atcactttaa ccccagagtt actctttaaa ctagcggctt ataccttgct taagcaagga
     2341 gataatgggt ctatggggga tattgcctgt atcgcttacc agactttagt cgcctatacc
     2401 tcctttgacc gagaacaggt cagtaactgg ctgcagacca tgcccttaaa aaaacttctc
     2461 gtcaaagatt ggggttacca tatccaagtc attcaaccag ccctgccttg tgactttttg
     2521 gttggctgga ctaaaatacc tgctatttca aggcagatga ttcaacaagt gacagcgagc
     2581 attaccccag ctttcttaag aacaagttac cagctaacgc aatcagctat ggtagctttg
     2641 caagaaggtc ataaggaaga actcaagaaa agtttagcag gagcaagtca tctcctaaaa
     2701 gagcttcatt cagctatcta ccatcctaag ctagtaacct tggtagctgc ttgtcagaag
     2761 caagatgctg ttgctaaatc ttcaggttct ggtggtggag attgtggcat cgcgcttgcc
     2821 tttaatcaga atgccagaga tacccttatt tccaaatggc aagaagctga tatcgcatta
     2881 ctttatcaag aaaggtggga agagaatgac caaccgtaaa gatgatcaca tcaaatatgc
     2941 cctcaagtac caatcgcctt ataatgcttt tgatgacata gaactcatac atcattcctt
     3001 acctagctat gatttgtctg atattgatct cagtactcat tttgccgggc aagacttcga
     3061 ctttcccttt tacatcaatg ccatgacagg aggaagtcaa aaaggcaaag ctgtcaatga
     3121 aaaattggcc aaagtagcag cagcaacagg gattgtcatg gtgacagggt cttatagcgc
     3181 tgctttaaaa aatcctaacg acgattccta tcgtttacat gaggtggcag ataacttgaa
     3241 actagccacg aatattggtc tagataaacc tgtggcgcta ggacaacaaa cggttcaaga
     3301 aatgcagccc ctctttttac aggttcatgt gaatgtgatg caagagttgc tgatgccaga
     3361 gggtgagcgc gtctttcata cctggaaaaa acacctcgct gaatacgcta gtcaaatacc
     3421 agttcctgtc attctcaaag aagttggttt tggcatggat gtcaatagta tcaagctagc
     3481 acatgaccta ggcattcaaa cctttgatat ttcaggaaga ggagggacct catttgccta
     3541 tattgaaaat caaagaggag gagaccgctc ttacttaaac gattggggac aaaccaccgt
     3601 tcagtgcttg ctaaatgcac aaggactgat ggaccaagtg gaaatcttag cttcgggtgg
     3661 tgtcagacac cccttggaca tgattaaatg tttcgtccta ggagcacgtg cagtgggact
     3721 ctcacgcacc gttttagaat tggtcgaaaa atacccaacc gagcgtgtga ttgctatcgt
     3781 taacggctgg aaagaagaat taaaaatcat tatgtgtgct cttgactgta aaactattaa
     3841 agaattaaag ggagtcgact acttactata tggacgcttg cagcaggtta attaggcttc
     3901 atcacctcta caatagtgcc acctactaca aagatgatag agctttttta gaacataata
     3961 taaaatattt ataaatacct catcaaactc agaatagagt agatgagtaa atagagtatg
     4021 ttatggagac tatttctaag acttgtatgt atagaaacaa agattaaaca cccatgtaaa
     4081 ccaattgttt acatgggtgt ttgccttata ttagttgttt aaatctaaga ctgtcttagc
     4141 tgggataagt aaaaatgtgc tttttctaga tttattggtt tatcagctag taaagcatta
     4201 actaaaggag caatttcgtc ttgggttgcc cccgccaaaa gagctaatga tttcgcatgt
     4261 agtttcatgt gaccagtttg gatgccagat gaggtcagag cttttagtgc agcaaagttt
     4321 tgagctaacc ccacagatgc aatcaattgg gctaatgttt tggcatcagg ttgattaaga
     4381 agatcatgtg cgatagaaac agtagggtta agcccgatag atcccccctt actagcaata
     4441 ggcataggga gggtcatttg gccaagcaga acttgtttat cttggtcaaa atgccactga
     4501 ctaagtcctt gatagctacc atttcttgag gcataagcat gggcccctgc ttcaatagca
     4561 cgccagtcat ttcctgtggc tattaccact gcatcaatgc cattaaaaat acctttatta
     4621 tgagtagtag cccggtaagg atctacttga gctagtcggc tggctagctc tattttttga
     4681 gctaactgta gggattttgt tttatcgtga tctaaaaggc gcacgggaat ctcacaacta
     4741 gtagtaacaa gcgactctgt tgcgtaatta gataaaatcg ccattagaca atgcccctta
     4801 gacagtcttg ttaaatcagg aacaagagct tccatcatag tattgaccat atttgctccc
     4861 atagcttctt gggtatcaac agtcagatag aaaatcaaaa actctccttt ttcttccaaa
     4921 tgaatggttc tagcgcctcc acctctttta acaatggaag gataagcttt atttgccaat
     4981 gcaattagct tttttgtttt atgaaggatg gcggctttag cgttatctat ttggtcgata
     5041 tcgtaaagaa caatttgacc aatcatttgt cggtttaggg tttgagcttt aaaaccacct
     5101 gagcgtttga ttagttttgc tgcgaaagat gctgcagcaa caacagaagg ttcttccgtg
     5161 acaaaaggca tctgataagt tgaaccgttg acaagaaaat caggagctat gctaaagggc
     5221 aaagccaatc gtcctaagac attttcagtc atttgattag ctgtttggat aggcaaaaga
     5281 acgtctgttt tgagttgatt taagttttca gcattaagta gtttaaattt ttcgataagt
     5341 tggaggcgtt cttcgaatgt tttctttgaa aaaccgctcc agttaagatt agtttttgtc
     5401 attggtatga tagatccttc gatgctcctt gatggctgtt agagcaaatt ttccagttaa
     5461 gtaaatgtca aaattggcat taccattatc atctaattgg acttgttcgt agaaaaggtc
     5521 ttcgtattta gaaacggaaa gacgtgttcg ttgatccagt aaagcttgtc gattagtcat
     5581 taacatttta tcgtacccag caactaactg accggaataa aattcactta cggcaccgga
     5641 accatagcta aaaaaaccaa ttttatctcc agattgtaat actttactat tttcaagtaa
     5701 agataacaat cctagataaa gtgagccagt ataaatattt ccaatttgtt tgctataagt
     5761 aatagaagct tgaaaggctt ctattagttt ttccctgtgt tcaggaggta ctgtgttatc
     5821 cataatgtta tttagacctt ttagggctaa cttgggaaat ggaatatgga aacaaactgc
     5881 agcaaaatca gataactgaa ggttttctct tttctgataa gcttgccacg tggtttctaa
     5941 gcaattgaga tattgcttgg tagagtaaat accatcaaca taaggtgtga aactatagtt
     6001 aggcctccaa aagtccataa tatccctagt ttgagccaca ttatcttcat ttagagcaag
     6061 aatacgaggg ttagcagtta ccaaaagagc aatactacca gagccttgag ttgattcgcc
     6121 aggagatcct acaccgtatc tagcaatatc actagcaata acaaggacac gagaatctgg
     6181 cttagaagcc acatgcagtt tagcatagtc tagagcagca gttgcgctat agcaggcttg
     6241 ttttacttca aaggaacgtg caaaaggctg gataccaact aagtgatgta catagatagc
     6301 tgatgccttt gactgatcag tactcgattc agttgccaaa ataaccatat caatcttaga
     6361 tcgatcttcg tcggttaaga tttgatctgc tgcagaagca gctaaagtaa taatgtcttc
     6421 agtgattggt gccacactaa aagactcaat gagtagccct tgactaaatt ttgctggatc
     6481 cacttggcgt gcaagcgcta aatcttccaa tttcaagaca tattgactgg ttgcaaaacc
     6541 aatcttatca attccaattg tcatatttgt ccttctttta gttattttta tagtcttacc
     6601 cattctatca cattttactg caaaattaga ctgataaagt tatttgtttt atcctttaat
     6661 agttaaaatg ttcatgacca catga
//
LOCUS       DATUYR010000072         6684 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.072, whole genome shotgun sequence.
ACCESSION   DATUYR010000072 DATUYR010000000
VERSION     DATUYR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.072"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..840
                     /gene="dnaN"
                     /locus_tag="VCI33_001477"
     CDS             <1..840
                     /gene="dnaN"
                     /locus_tag="VCI33_001477"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEP2832396.1"
                     /translation="EQHQVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLK
                     SIIAETAFAASLQESRPILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDV
                     VIPSKSLREFSAVFTDDIETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTE
                     FETEVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDI
                     VSQSGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPV
                     RTN"
     gene            915..1112
                     /locus_tag="VCI33_001478"
     CDS             915..1112
                     /locus_tag="VCI33_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEP2832397.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1442..2557
                     /gene="ychF"
                     /locus_tag="VCI33_001479"
     CDS             1442..2557
                     /gene="ychF"
                     /locus_tag="VCI33_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEP2832398.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2627..3196
                     /locus_tag="VCI33_001480"
     CDS             2627..3196
                     /locus_tag="VCI33_001480"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEP2832399.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            3199..>6684
                     /gene="mfd"
                     /locus_tag="VCI33_001481"
     CDS             3199..>6684
                     /gene="mfd"
                     /locus_tag="VCI33_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEP2832400.1"
                     /translation="MNILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLGNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GVFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFADNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYKRLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEREIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYISDERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKN"
BASE COUNT         2259 a         1032 c         1311 g         2082 t
ORIGIN      
        1 gaacaacacc aagttgtttt aaccagtggt aaatcagaga ttaccttaaa aggaaaagat
       61 gttgaccagt atcctcgtct acaagaagta tcaacagaaa atcctttgat tttaaaaaca
      121 aaattattga agtctattat tgctgaaaca gcttttgcag ccagtttaca agaaagtcgt
      181 cctattttaa caggagttca tattgtatta agcaatcata aagattttaa agcagtagcg
      241 actgactctc atcgtatgag ccaacgttta atcactttgg acaatacttc agcagatttt
      301 gatgtggtta ttccaagtaa atctttgaga gaattttcag cagtatttac agatgatatt
      361 gagaccgttg aggtattttt ctcaccaagc caaatcttgt tcagaagtga acacatttct
      421 ttttatacac gcctcttaga aggaaattat cccgatacag atcgtttatt aatgacagaa
      481 tttgagacgg aggttgtttt caatacccaa tcccttcgcc acgctatgga acgtgccttc
      541 ttgatttcta atgctactca aaatggtact gttaagcttg agattactca aaatcatatt
      601 tcagctcatg ttaactcacc tgaggttggt aaggtaaacg aggatttaga tattgttagt
      661 cagtctggta gtgatttaac tatcagcttc aatccaactt accttattga gtctttaaaa
      721 gctattaaaa gtgaaacagt aaaaattcat ttcttatcac cagttcgacc attcacccta
      781 acaccaggcg atgaggaaga aagttttatc caattaatta caccagtacg aacaaactaa
      841 gtaagaaaaa gctccctttt aggagttttt ttgttattat aaatattaac gataatataa
      901 gtggagaaaa gggaatgtat caaattggat catttgttga aatgaaaaaa cctcatgctt
      961 gtgtaattaa agaaactggt aaaaaggcta atcaatggaa ggtactgaga gtaggagctg
     1021 atattaaaat tcagtgtact aactgtcagc atgtgattat gatgagtcgt tatgattttg
     1081 agcgaaaatt aaaaaaagta ctgcaaccat gaaagtcctt ttaaataaat aattctagct
     1141 atatttgcaa ccaatacttt cctaaaaaat tgctagtatg ccgttggaaa attagctatt
     1201 ctaatgttat cgaaagaaga aagatggcca ttgacaatga cgaaagttgt agcacagtta
     1261 cttagtttct cggcaaacta tatcaaaatg agaataatac accaaacttt gataatttaa
     1321 atgaaggtga aagagtggca taatgataag caaatctgaa attttcttct tattcatacc
     1381 gctttttctt ctattttttg gtataataat cttgattgaa atttgaatgg agattcgcta
     1441 aatggcttta acagcaggta ttgtaggctt accaaatgtt ggtaaatcaa ccttatttaa
     1501 cgcaattaca aaagcaggag cagaagctgc taattatcct tttgcgacta ttgatcccaa
     1561 tgttggtatg gtagaggttc cagatgagcg tctgcaaaaa ttgacagaat tgattacacc
     1621 taaaaaaaca gttccgacaa cctttgaatt tactgatatt gcaggtatcg ttaaaggtgc
     1681 ttctagaggg gaaggtctag gaaataaatt tttagctaat attcgtgaag tcgatgctat
     1741 tgttcatgtg gtacgtgctt ttgatgatga aaatgtcatg cgtgaacaag gtcgtgagga
     1801 tgctttcgtt gatcctatag cagatattga cactattaat cttgaattaa tcttagctga
     1861 tttagagtca atcaataaac gttatgcgcg tgttgaaaaa atggcacgaa ctcaaaaaga
     1921 taaagaatca gtagcagagt ttaatgtttt gcaaaagatt aaacctgttt tggaagatgg
     1981 gaaatcagct aggacaattg agtttacaga agatgaagca aaagttgtta aaggtctctt
     2041 tttattaaca actaaacctg ttttatatgt agctaatgtc gatgaagata aagttgctaa
     2101 tccagatggt attgattatg tcaaacaaat tcgtgacttt gcagctactg aaaatgctga
     2161 agtagttgtt atctcagcgc gtgcagaaga agaaatttca gagcttgacg atgaggataa
     2221 agaagaattt ttggaagcta tcggtcttac tgaatcaggc gttgataaat taaccagagc
     2281 agcttatcat ctcttaggcc ttggaaccta ttttacagca ggtgaaaaag aggttcgtgc
     2341 ttggacgttt aagcgtggta taaaagctcc acaagctgct ggtattatcc attcagattt
     2401 tgaaagaggt tttattcgtg cagtaaccat gtcttatgat gatctaatga cctacggttc
     2461 agaaaaagcc gtcaaagaag ctggacgctt gcgtgaagaa ggaaaagaat atgttgtcca
     2521 agatggggac atcatggaat tcagatttaa tgtgtaatta tattaaaaca atatcagaag
     2581 gttggaagaa cattccagcc cttttggcat tttagaaaga gaaaatatgg taaaaatgat
     2641 tgttggtctg ggaaatccag gctctaaata tgaaaaaaca aagcacaata ttggttttat
     2701 ggctattgac aatattgtca agaaccttga cgttaccttt acagatgata aaaattttaa
     2761 agcacagata ggaagtactt ttattaatca tgaaaaagtt tactttgtga aacctactac
     2821 ttttatgaat aatagcggca tagcagtaaa agcattacta acctactata atattgacat
     2881 aacagattta attgttatct atgatgattt agacatggaa gtcagtaaat tacgtttacg
     2941 tagtaagggt tcagcaggag gacataatgg cattaagtca atcattgccc acattggaac
     3001 tcaggaattt aaccgaatca aagttggtat tggacgacct ttaaaaggta tgactgttat
     3061 taaccatgtg atgggccaat tcaataccga agataatatt gctatttcgt taactcttga
     3121 cagagttgtc aatgctgtca agttttattt acaagaaaat gattttgaaa aaacaatgca
     3181 gaaatttaat ggataatcat gaatatttta gaattattta gtcagaataa gaaagtccaa
     3241 tcctggcact ctggattaac caccttagga agacaactgg taatggggtt atcgggttca
     3301 agtaaagcat tggctatagc ttccgcttat ttagatgatc aaaaaaaaat agttgtggtt
     3361 acatcaactc aaaatgaggt tgaaaaatta gccagcgatt tatctagttt acttgatgaa
     3421 gaacttgttt tccaattttt tgcagacgat gtggctgcag cggaatttat ctttgcgtca
     3481 atggataaag ctctatcaag aatagaaacc ctgcaatttt tagggaatcc taaatctcag
     3541 ggcgttttaa ttgttagttt atcaggctta agaactttat tgccaaaccc agatgttttt
     3601 acaaagagtc agattcaact aacagttgga gaagattatg atagtgatac tcttactaaa
     3661 caactgatga caattggcta tcagaaggtc tcacaggtca ttagtccggg agaatttagc
     3721 cgtcgagggg atattttaga tatctatgag attacacaag aattgcctta tcgattggaa
     3781 ttttttggcg atgatattga tagtattagg caatttcatc cagaaactca aaaatctttt
     3841 gaacaactag aaggtgtttt tattaatcca gcaagtgatc ttatttttga ggctagtgat
     3901 tttcaacgtg gcattgagca attagaaaag gctctacaaa cagcacaaga tgataaaaaa
     3961 tcttatttag aagatgtatt agctgtttca aaaaacggtt ttaaacataa ggatatccgt
     4021 aaatttcaat cattatttta cgaaaaagag tggtcattat tagattatat tcctaaggga
     4081 acgccaatct tttttgatga ttttcaaaaa ctagttgata aaaatgcaag atttgattta
     4141 gagattgcta atctcttgac agaagattta cagcaaggaa aggctctttc caatcttaac
     4201 tactttgcag ataattatcg agagcttagg cactataagc cagcgacctt cttttcaaat
     4261 tttcataagg gacttggaaa tatcaaattt gatcagatgc atcagctaac tcagtatgcc
     4321 atgcaggaat tttttaatca atttcctttg ttgattgatg agattaaacg gtatcaaaaa
     4381 aatcaaacaa cggttattgt acaggtagag tctcagtatg cttacaaacg acttgaaaaa
     4441 tcttttcaag attaccaatt tcgccttcct ttagtgagtg ctaatcaaat tgtttcacgt
     4501 gaatcacaaa ttgtaattgg agctatctcc agtggttttt attttgctga tgaaaagtta
     4561 gcgttaatca cagagcgtga aatttatcat aaaaagatca aacgacgcgc tagacgatct
     4621 aatattagca atgctgagcg tttgaaagat tacaatgagt tagcagtagg tgattacgtg
     4681 gttcataatg tccatggtat cggtcgcttt cttggaattg aaacgattca gattcaggga
     4741 atccatcgag attatgttac tattcaatat caaaattcag accgtatttc tcttccaatt
     4801 gaccaaattg gtagcttatc aaagtacgtt tctgctgatg gaaaagaacc taaaattaat
     4861 aaactcaatg atggtcgttt ccaaaagaca aagcaaaagg tcgctagaca agtagaggat
     4921 attgctgatg accttctaaa attatatgct gaaagaagtc agcaaaaagg attttcattt
     4981 tcaccagatg atgacttgca gcgcgctttt gatgatgatt ttgcttttgt agaaacagaa
     5041 gatcaactta ggtctataaa ggaaattaaa gctgatatgg agagcatgca gcctatggat
     5101 cgtcttttag taggcgatgt aggatttggt aagacagaag tagctatgag ggcagccttt
     5161 aaagcggtga acgatcacaa acaagtagct gtcttagttc caaccacagt cttggcccag
     5221 cagcattatg aaaacttcaa agcacgcttt gaaaattacc ctgttgaggt tgacgtctta
     5281 agtcgtttcc gtagtaaaaa agagcaagct gaaacactag aacgtgtacg aaaaggtcaa
     5341 attgacatta ttatcggaac ccatcgactc ctatcaaaag atgtggtctt ttctgattta
     5401 ggattgattg tgattgatga ggaacaacgg tttggtgtta agcataaaga aactttaaag
     5461 gaattaaaaa ctaaggttga cgtcttaacc ttaacagcta ctccgattcc tagaacctta
     5521 cacatgtcta tgttaggtat ccgagattta tcggttattg agaccccacc aaccaatcgt
     5581 tatcctgttc aaacctatgt tttggaaaat aatccaggtc tcgttagaga agctatcatt
     5641 cgtgaaatgg atcgtggagg acaaattttt tacgtttaca ataaagttga cactattgaa
     5701 aagaaagttt cagagctaca agaattagtc ccagaagctt ctataggttt tgttcatggg
     5761 caaatgagtg aaattcaact tgaaaatacc ttgattgact ttataaatgg tgattatgat
     5821 gtccttgtgg ctacaacaat catcgaaaca ggagttgaca tttctaatgt aaacactttg
     5881 tttattgaga atgctgatca tatgggattg tcaactttat atcaactacg agggcgcgtc
     5941 ggaagaagta atcgtattgc ttatgcttac ctgatgtatc gtcctgataa ggtcctaaca
     6001 gaggtttctg aaaagcgttt agaggctatt aaaggcttta ctgaattagg ttcaggcttc
     6061 aagattgcta tgcgagattt gtctatacga ggagcaggta atattttagg ggcttctcag
     6121 agtggcttta ttgattcggt cggttttgaa atgtattctc agttattgga acaggctatt
     6181 gctagcaagc aaggaaaaac gactgttcgc caaaaaggta atactgaaat caatcttcag
     6241 attgacgcct atttgccaga tgattacata tcagatgagc gtcaaaaaat tgacatttac
     6301 aagcgtattc gagaaattca atcaagagaa gattatctca atttgcagga tgagctgata
     6361 gatcgttttg gagagtatcc tgatcaagtt gcctatttgt tagagatagc tctgctaaaa
     6421 cattatatgg acaatgcctt tgcggaatta gttgaacgca aaaataatca ggtcattgtt
     6481 cgatttgaag taacttcttt aacttatttc ttgactcagg attatttcga agccttatcg
     6541 aaaacccatc ttaaagctaa aattagtgaa catcagggga aaatcgatat cgtctttgac
     6601 gttcgccatc aaaaagatta tagaatttta gaagaattga tgttatttgg agaaaggctt
     6661 agtgagataa aaatcagaaa aaac
//
LOCUS       DATUYR010000073         6565 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.073, whole genome shotgun sequence.
ACCESSION   DATUYR010000073 DATUYR010000000
VERSION     DATUYR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.073"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..942
                     /locus_tag="VCI33_001482"
     CDS             <1..942
                     /locus_tag="VCI33_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP2832401.1"
                     /translation="LTISKIVYQKKFFHLAKKSEKVISTYHVTDDLKPFQVRDLVSGH
                     LERIQEEVEKKYPNAGFNSDKYNGLKESNSLLSDGFEVKSGNLIFDKKLTIPLTTLFD
                     VINPDFLANSDRAAYDNYRTYKEQHPKKLVALTFDDGPDPTTTPQVLDILAKYQAKGT
                     FFMIGSKVVNNENLTKRVSDAGHEIANHTWDHPNLTNLSVSEIQHQVNMTNQAIEKAC
                     GKKPRYLRPPYGATNATVQQSSGLTQMLWTVDTRDWENHSTDGIMTNVKNQLQPGGVV
                     LMHDIHQTTINALPAVMEYLKAEGYECVTVSELYAHQ"
     gene            complement(1052..2137)
                     /locus_tag="VCI33_001483"
     CDS             complement(1052..2137)
                     /locus_tag="VCI33_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP2832402.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2235..2861
                     /gene="udk"
                     /locus_tag="VCI33_001484"
     CDS             2235..2861
                     /gene="udk"
                     /locus_tag="VCI33_001484"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEP2832403.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            2941..3204
                     /locus_tag="VCI33_001485"
     CDS             2941..3204
                     /locus_tag="VCI33_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEP2832404.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3257..4066)
                     /locus_tag="VCI33_001486"
     CDS             complement(3257..4066)
                     /locus_tag="VCI33_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEP2832405.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     AEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4211..4708
                     /locus_tag="VCI33_001487"
     CDS             4211..4708
                     /locus_tag="VCI33_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEP2832406.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
     gene            4708..6393
                     /gene="dnaX"
                     /locus_tag="VCI33_001488"
     CDS             4708..6393
                     /gene="dnaX"
                     /locus_tag="VCI33_001488"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEP2832407.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISMLALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAKEEGVALPSGLSSDVTTEVEGLKRELNQLKQALAQLQASPS
                     PSHAPVKPAQAKPKLTTYKIDRVKILKIMEETVQDSQQSRQYLDALKTAWNEILDSIS
                     AQDRALLMGSEPVLANSENAILAFEAAFNAEQAMKRTDLNAIFGNIMSKAAGFSPNIL
                     AVPKADFHHIRSEFALQLKTQKASDPTEETIEETLEIPEGFDFLVDKINAVED"
BASE COUNT         2119 a         1104 c         1344 g         1998 t
ORIGIN      
        1 ttgactattt ctaaaatagt ttatcagaaa aaattttttc atttggctaa aaaatcagaa
       61 aaagttataa gtacatatca cgttacagac gacttgaaac cgtttcaggt aagggatcta
      121 gtatcaggac atttagaaag aatacaagaa gaagttgaaa aaaaatatcc aaatgctggt
      181 tttaatagcg ataagtacaa tggcttaaaa gaatctaatt ctttattaag cgatggcttt
      241 gaggtaaaat cgggaaacct tatttttgat aaaaagctaa cgataccttt gacgacatta
      301 tttgatgtta ttaatccaga ttttttagca aatagcgata gagctgcgta tgataattat
      361 aggacctaca aagaacagca tcccaaaaaa ctagttgcat taacgtttga tgatggtcca
      421 gatccgacga cgactcctca agttttagat attttggcaa aataccaggc taagggaact
      481 ttctttatga taggttcaaa agttgtgaat aatgaaaacc ttactaaacg tgttagcgac
      541 gctggccatg aaattgctaa tcatacttgg gatcatccca atctgactaa tctttcagtc
      601 agcgagattc aacatcaagt taatatgacg aaccaggcta ttgaaaaagc ttgtggcaag
      661 aaacctcgct atttacgccc tccatacgga gctacaaatg caactgttca gcaatcctca
      721 ggattaacac aaatgctttg gacggttgat acaagagatt gggaaaatca tagcactgat
      781 ggcattatga ctaatgtcaa aaaccaattg caaccgggag gagttgtttt aatgcatgat
      841 attcatcaaa caactatcaa tgctttgccg gcagttatgg aatatctaaa agcagaagga
      901 tacgaatgtg tgactgtatc agaactctat gcgcatcagt aagtaatatc gttatgtttc
      961 tttagaattg atatactaaa caatccaatt actttgaagt aattggattg ttgtttttag
     1021 tagcccttga ttaagttttg ttttttaaca actagttgat gaaatgaagt tgttgatttt
     1081 tgagggagat ttcagagact ttagcaaatt ttttcaattt tttgaggtct tcagggtggc
     1141 taacaaaggt aataacgata ccagattttc ccatacggcc ggttcgtcca gctctgtgag
     1201 tataattttc cttatcacga gcaacatcaa aatgaatgac atagtcaaga ttatcaatat
     1261 caatgccccg agcaacaaga tccgtagcta acaataagga gagttggtgg gatttgaatt
     1321 tttcgagaat ggttttacgg aatttgacat tgatgtcact cgctaaggaa accgcagcag
     1381 ctccattata ttggagacgt tcttccgtag cacccaaatc agataaactg ttgaaaaaga
     1441 ctaaagctct aaaatgagga atattagcaa attttcgtaa taaatcggta cgttcgcgct
     1501 tgtcaaccat gaggtaaaaa tgctgaatag catcgttggt ttgctctgaa agatcgatgc
     1561 agaatgtatt tggagctaag gaagtttgat ccaccttatt agtagcactc atgtatacca
     1621 tttgatgatc tcgaggaaca tagtggctaa ttttttgaac aaaatcatat tgggaatcac
     1681 ctaatagttc gtcatattca tctaaaacaa tggtgttgac agacatcatc ttgatttttt
     1741 tcaatttaat cagttcaaaa atgcgtccag gggtcccaat caggatttca ggaccttttt
     1801 tgaggcgttc gatttgacgt ttttgactgg ttccagaaat aaagagttgt gccgttaagc
     1861 ctagtggctg tgcccaatcc tttgtgactt caaaaatttg gccagctaat tcggtattag
     1921 gggctaaaat gagtagttgt tgggactttt taggcgtcag tgccaataga ctaggaaaga
     1981 cataagctaa ggtcttgccc gtcccagtag ggctaatacc aaggaaattt ttaccatcaa
     2041 caataggttg aaaggcctgt tcttggattg gcgttaaatg ggtaaatgca acttggtcaa
     2101 gtttttcttg ccattgaggg ggaaatttag tgatcatgcg tcttcctttt actattgttt
     2161 ctatattcat tatagcatta tagtctcaat tttggtaaaa tagtgagaaa gactttttag
     2221 atttggagtt taaaatgctt aaaaaaccga ttattattgg tgtaactggt ggttcaggtg
     2281 gcggtaaaac aagtgtgtca cgcgctattt tggacagttt tccaaatgct cgtattgcga
     2341 tgattcaaca cgattcttac tataaagatc agtctcacat gagttttgaa gaaagggtca
     2401 aaaccaatta tgaccatcct ctagcttttg atacagattt tatgattcag caactaaaag
     2461 aattattagc tggtagacct gtagatattc ctatctatga ttacaaaaag catacccgca
     2521 gtaatacgac ctttcgtcag gatcctcagg atgttattat tgttgaaggt attcttgtcc
     2581 tagaagatga gcgtttgcga gatttgatgg atatcaagct ctttgtagac acagatgacg
     2641 atattcgtat tattcgtcgt attaagcgtg acatgatgga acgtggcaga agcttagaaa
     2701 gtattattga tcagtacaca agcgtggtaa agcctatgta tcatcagttt attgagccaa
     2761 gtaaacgcta tgctgatatt gttattccag agggagttag taatgtcgtt gctattgatg
     2821 ttattaatag taaaattgcg agtattcttg gagaagtata gtggaagtgg ctgagtagat
     2881 tatttaaatc tgctctttta ttgttgtgct cttcatagag aaagggatca taaaatagaa
     2941 atgttattaa tttggcttgg attactattt gtttggaatg aactcgtgtt tgtcatatat
     3001 gcctttgata aaagaaaagc cataaaaaag aaacggcgta ttagtgaaag aaacttatta
     3061 gtgataacag ttttgtttgg aggttttgga gcattacttg cagctaaaaa gtaccaccat
     3121 aaaaccagaa agtggtactt tgtcatcact tggtatacta gtattttgtt gacattacta
     3181 gtaacttata tctttatgaa gtgagtcact taaaagaccc ttaatcagta ttatttagtc
     3241 aaggctcttt tatagtttaa aaaagcacat catcgtcaag ggtgttgatt ttttgaaaaa
     3301 gatcaccaaa gattaaaagg attaatgttc ctagtaaaac tgtcatgcca ggaatgcttg
     3361 ttagaggaga gttccagatg aaatgtagca ccagaggact taataaccag aaaatttgtt
     3421 gctgttttga aaaaaggtta gaacccttta ataataagta tgctccaatg gtaaaaatag
     3481 ctgtatgtac ccaatgtgaa gtcacagcag tgctaatacg ttcaatagca gtcggtacga
     3541 gactatctat agattttgct tgaatcaaat aacggatatc ttctgctagt tgaaagccca
     3601 tgccagaaag taaagcaaca actaattttt ctttaagagt aagtctagga aataaagtaa
     3661 ttactaaaat agccgaagaa gccttaatgg gctcttcgac aataggggca gttaaagcat
     3721 ctccccaaag ctttaaagcg tctttagaag ggatgatgta tgaccaaaat tgtcctacga
     3781 gatggttccc acaagaagct aaaaaacctg aaatataaag tccgctaaga cagctaacaa
     3841 taataacatt caggttcata tgaaattttt tacttaaata aaaaatagct gctgcaaatg
     3901 gaattaagta aatgcctaat agactaatgg ttagtattag agcaaaagct tctttaaagg
     3961 aaaggttgtt agctgtcagt cctaacaatt caagttcaat accatttaaa actaaagcaa
     4021 taaaaagacc gtatcgtaac catttctgtt gagtgatttg tttcacgaaa acctcctaat
     4081 attaagatga tattaataat attgaaaatt atatcataaa caacctcaaa aaccatttta
     4141 aaaattgact gaagcgaaag atagtcgtaa aaaatactcg aatagaccca gatatgctat
     4201 aataaaaggt atgaataagt ctaaaaaaat tgagcaatac caactaatga tagcacaggc
     4261 aaaagagctg tttgcgaatg aatcaaatgc tttggctaat ttgtcaaatg ccagcgccct
     4321 gttaaacatg acactaccca attctgtttt tgcgggattt tacctttttg atgggcagga
     4381 attaattctt ggcccttttc aaggtagggt ttcttgcgtc catatcaagc ttggtaaagg
     4441 tgtttgtggc gaatcagctc agtctcgtcg aaccattatt ataaatgatg tgaagcagca
     4501 tgccaactat atttcctgtg atgcggcagc catgagtgaa attgttgtgc ctatggtgaa
     4561 agaaggccat ttgattgggg tgctggactt ggattctagc cttgtagctg attatgatga
     4621 ggttgatcaa gaatacttag aggcctttgt ggacctattt cttgaaaaga ccacgtttac
     4681 ctttaacatg tttggagtta aaaactgatg tatcaagctc tttatcggaa ataccggagc
     4741 caaacgtttg acgaaatggt gggacaatcg gttatttcca caactttaaa gcaggcagtt
     4801 gaatctggca agattagcca tgcttatctt ttttcaggtc ctagagggac tgggaaaaca
     4861 agtgcggcaa agatttttgc aaaggccatg aattgtccta accaagtcga tggtgaaccc
     4921 tgtaatcaat gcgatatttg ccgagatatc acgaatggaa gcttggaaga tgtgattgaa
     4981 attgatgctg cctcgaataa tggtgttgat gaaattcgtg acattcgaga caaatcaacc
     5041 tatgcgccaa gtcgtgcgac ttataaggtt tatattattg atgaggttca catgttatca
     5101 acaggggctt ttaatgcgct tttgaaaact ttggaagaac cgacagaaaa tgttgtcttt
     5161 atcttggcaa caacggaatt gcataaaatt ccagccacta ttttatctcg tgtgcaacgc
     5221 tttgaattca aagctattaa gcaaaaagct attcgagagc atttagcctg ggttttggac
     5281 aaagaaggta ttgcctatga ggtggatgct ttaaatctca ttgcaaggcg agcagaagga
     5341 ggcatgcgtg atgctttatc tattttagat caggctttga gcttgtcacc agataatcag
     5401 gtcaccattg caattgccga agaaattaca ggttctattt ccatgcttgc tctgggtgac
     5461 tatgttcgat atgtctccca agaacaggct acgcaagctc tggcagcctt agagaccatt
     5521 tatgatagtg ggaagagcat gagccgcttt gcgacagatt tattgaccta tctgcgtgat
     5581 ttattggtgg ttaaagctgg cggtgacaat cagcgtcagt cagctgtttt tgataccaat
     5641 ttgtctctct cgatagatcg tatattccaa atgataacag ttgttactag tcatctccct
     5701 gaaatcaaaa agggaaccca tcctcggatt tatgccgaaa tgatgactat tcagctagct
     5761 aaagaagaag gagtagcgtt accatcaggt ttgtcatcag atgtgacgac tgaagttgag
     5821 ggacttaaac gtgaacttaa tcagcttaag caagccttgg cacaattgca agccagtccc
     5881 agtccctcac atgctcctgt aaaaccagca caggctaaac cgaaactaac cacttacaag
     5941 attgatcggg tgaaaatact gaaaatcatg gaagaaacgg ttcaagatag tcagcaatca
     6001 cgccaatact tggatgccct aaaaacagct tggaatgaaa ttttagacag tatctcagct
     6061 caagatagag ccttactaat gggatcagag cctgttctgg ccaatagtga gaatgctatt
     6121 ttagcttttg aagctgcttt taatgctgag caagcaatga agcgtactga tttgaatgcg
     6181 atattcggca acattatgag caaagcagca ggcttctcac ctaatatttt ggcagtccct
     6241 aaagctgatt ttcatcacat tcgttctgag tttgccttac aattgaaaac tcaaaaggct
     6301 tctgacccta cagaagagac gatagaagaa acccttgaga ttccagaagg ctttgatttt
     6361 ctagttgata aaattaatgc cgttgaggat taaacattat tagctgttaa tgaaccagta
     6421 acgctataaa ggtaagccta ttgggcttac ctttagcgtt tttttatcta atataccaag
     6481 atagtttttc tagccaatcc tctaatagta gaagtgaaac caaaaaagat tgatgctttc
     6541 ttgggtatcc aagtcagcat caatc
//
LOCUS       DATUYR010000074         6362 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.074, whole genome shotgun sequence.
ACCESSION   DATUYR010000074 DATUYR010000000
VERSION     DATUYR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.074"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            517..1044
                     /locus_tag="VCI33_001489"
     CDS             517..1044
                     /locus_tag="VCI33_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEP2832408.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1044..2162
                     /gene="yqeH"
                     /locus_tag="VCI33_001490"
     CDS             1044..2162
                     /gene="yqeH"
                     /locus_tag="VCI33_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEP2832409.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPGKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2188..2496
                     /gene="yhbY"
                     /locus_tag="VCI33_001491"
     CDS             2188..2496
                     /gene="yhbY"
                     /locus_tag="VCI33_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEP2832410.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2565..3197
                     /locus_tag="VCI33_001492"
     CDS             2565..3197
                     /locus_tag="VCI33_001492"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEP2832411.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDFYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3194..3787
                     /gene="yqeK"
                     /locus_tag="VCI33_001493"
     CDS             3194..3787
                     /gene="yqeK"
                     /locus_tag="VCI33_001493"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEP2832412.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            3811..4164
                     /gene="rsfS"
                     /locus_tag="VCI33_001494"
     CDS             3811..4164
                     /gene="rsfS"
                     /locus_tag="VCI33_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEP2832413.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWYEAPAVALDAYLA"
     gene            4212..4955
                     /locus_tag="VCI33_001495"
     CDS             4212..4955
                     /locus_tag="VCI33_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP2832414.1"
                     /translation="MKHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTKTSKRWFFVAYK"
     gene            5209..6315
                     /locus_tag="VCI33_001496"
     CDS             5209..6315
                     /locus_tag="VCI33_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEP2832415.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTEDVID
                     YQKLIKVYEKKSKQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
BASE COUNT         1995 a          974 c         1372 g         2021 t
ORIGIN      
        1 gtaatcattt tttgatttac agttattatc atgtgttatc tggcgactgt cctgtttaca
       61 agtaagttca caagtatggg ataaaatagt ataaactata gtaaatactg aatagtcata
      121 gaaaaatatc ggcataggtc agaaaagctg atatgtttaa tactttttgc ggatatctac
      181 tgtatttaaa tgtatttgat atccttgtca aggatatacg tctaaacaag ttcaaaacga
      241 aatcatttgt gattatctta tcaggagttg actcttgata agatttttgt atatcagtac
      301 tgtggtacgt ttagatagct tggcagtgaa tttatagagt gtcaaacaac gagtgttttg
      361 tgttacacta accattagaa gagtctattg ttgagcatat caaggtgtta tcttttcgag
      421 ttgaggtatc atatctagta tatttaacta tggttttaaa tgctatttca aaaaaaatat
      481 gataaaatag aaagtactaa ttattttgga gatgacatga gtatcgatga ttataggccg
      541 acctatatgg tagaggctat ttatgattta agagcaaatg atttgttgcg tcatggtatt
      601 acggctgttt tagtggattt agataatacc ttaattgcat ggaataatcc tgatggcaca
      661 ccagaggttc gcgcttggct tgatgaaatg actatcgcgg atattagcgt ggttgttgtt
      721 tcaaataata aacattctcg tgttgagcgt gccgtttcac gctttggtgt tgactttatt
      781 agccgggctt tgaagccatt tgcttatggt attgaaaaag ccattgctcg ttatggattt
      841 gatcgcaacg aggttatcat ggttggagat cagctaatga ctgatatcag agctagtcat
      901 agagctggaa taaaatcagt tttagtcaaa ccacttgttg cttctgatgc ttggaatact
      961 aaaattaacc gctggcgtga acgccgtgtg atggcaaaat tagaagagaa atatggtaaa
     1021 ttatcatatc aaaaaggaat ttaatggaag aattattttg tataggctgt ggtatccaga
     1081 ttcaaactga agacaaagaa aaagctggtt ttacgccagc tgctgctttg aaaaagggta
     1141 tggaaacagg agaactctat tgtcagcgct gctttagatt gaggcattat aatgaaatta
     1201 cagatgtcca tattaccgat gatgaatttt taagacttct tcacgaagtg ggtgatagtg
     1261 atgctcttgt ggttaatgtg attgatattt ttgactttaa tggttctatt attccaggtc
     1321 tatctcgctt tatttctggt aatgatgtct tactggttgg gaataaaaaa gatattttgc
     1381 caaagtctgt taaggatggt aaagttactc aatggttgac ggaacgtgca cacgaagaag
     1441 gtcttcgccc tttagacgtt atgttaacga gcgctcaaaa taaatatgcg attaaagact
     1501 tgattgagcg tatcaatgag ttacgtaatg gtcgagatgt ttatgttgtg ggggtcacta
     1561 atgttggtaa atcgacctta attaatgcca ttattcaaga aattacaggg gataaagatg
     1621 tgattacgac gtcccgcttc cctggaacga cccttgataa aattgaaatc ccactggatg
     1681 acggtagctt catatttgat acgccaggta ttattcatcg tcatcaaatg gctcattatt
     1741 tatcccctaa agagctaaaa atagtgagtc ctaagaagga aatcaagcct aagacgtatc
     1801 aattaaaccc agaacaaact ctctttttag gtggtttgtc tcgctttgac tttatcaatg
     1861 gggagcgtca agggtttaca gctttttttg acaatcaact agaattacat cgtacaaaat
     1921 tagctggagc agatgctttt tatgataagc atgttggaac tttgttaact ccgccaggta
     1981 agaaagagtt gacagcattt cctaaattag ttcgtcatga atttacaatt gatcagaaaa
     2041 tggatattgt tttttctggt ctaggatgga ttcgtgttaa tggtcaaaaa gactcaaaag
     2101 caatagttgc tgcatgggct cctgaaggcg tcgctgtcat tgttcgtaaa gcgattattt
     2161 aaaaataaaa aaagaagttg gaaaaaaatg cttacaagta aacaaagagc tttcttaaaa
     2221 tctgaggctc atagcttaaa accgattgta caaattggca aaaatggatt aaatgaccac
     2281 attaagacta gtattcgtca agctttggat gctagagaat tgataaaggt cactttgttg
     2341 caaaatactg atgaagatat tcatgaagta gcagagattt tagaagaaga aattgggtgt
     2401 gatacggttt tgaaaattgg tcgtatcttg attctctata aggtttctgc taagaaagaa
     2461 aaccgcaagt tgtcccctaa agttaaagca atctagtcct tttatttgta tagggactgg
     2521 ttttatgaga cgaggactat tgtcgcgaga attttggaga aagtatggca ttagaattgt
     2581 tgacgccttt tacgaaggtg gaattagagg aagaaaaaaa agaaagcaac cgcaaacaga
     2641 ttgggatttt aggagggaat tttaatccta ttcacaatgc acaccttgtg gttgcagatc
     2701 aagttcgcca gcaattagga ttagatcagg tgctattgat gcctgaatgt aaaccaccgc
     2761 atgtggatgc caaagagact attgatgaga agcatcgtct tcgcatgttg gaattggcta
     2821 ttgaagatgt tgaagggcta gctattgaaa cctgtgaact tgaacgtcag gggatcagct
     2881 atacctatga tacgatgctt tatttgactg agcagcatcc tgatgttgat ttttatttta
     2941 tcatcggagc tgatatggtt gattatttgc caaagtggca ccgtattgat gaactggtca
     3001 aactcgttca atttgttgga gttcaacgtc caaagtataa agctggaacc tcttacccgg
     3061 tcatttgggt agatcttcct ttgatggata tttcttctag tatgattagg gattttatta
     3121 aaaaaggtcg tcagcccaat tatctcttac ctaagcgggt tttagactac attactcaag
     3181 aaggtttgta tcaatgacat atgaagatta tcttccttat agcaggaccg aactgttagc
     3241 aaaaattgca gagcagatga gccctaaacg gtttaaacat gtacttggcg tggaaaaggc
     3301 agccttgtcc ttggcagagt gttatggttg taacccagac aaagcagggt tagcagctct
     3361 tttacatgat tatgccaagg agtgccctga ccaggtcttt ttagacttaa ttgacaagta
     3421 ccaattatct ccagaattag ctaaatggaa taataatgtc tggcatggta tggttggtat
     3481 ttataagatt caagaagacc tagggctaaa agacaaagat atcttgcgag ctattgaaat
     3541 tcacaccgtt ggtgctgctg agatgacctt gctggataag gtcttatacg ttgctgatta
     3601 tattgaagaa ggtcgcattt ttcctttggt tgatgacgct agaaaaatcg cgaagctcga
     3661 tttgaatcag gctgttgcct atgaaacagt gaacactgtc gcttatttag cctcaaaagc
     3721 gcagcctatt ttcccacaaa ctctggatac ctataatgcg ttttgcagct atttaaagga
     3781 atactaatgg ataatgatat gaggaataaa atgaaaaaag aagaactctt aaaaattgtt
     3841 gtggaagcaa ctgatgaaaa gcgtgctaaa gatattttag ccttggattt agaggggtta
     3901 actagtttga ctgattattt tgtgattgcg agtgcaacaa atagtcgtca attagaggct
     3961 attgcggata atatccggga aaaggtaaaa gaggctggtg gagatgctag ccatgttgaa
     4021 ggtaatagcc aagcaggctg ggtcttgctt gatttgaccg atgtggtggt acatcttttt
     4081 ttagaagatg agcgttacca ctataatctt gaaaaattgt ggtatgaagc gcctgctgtg
     4141 gctctagatg cctatttagc ttaatgttac aaagagacct tgtcaatacg acagggtttt
     4201 tgaggatgat aatgaaacat aattatgaaa aatttgcgtc agtttatgat gctgtgatgg
     4261 atgattctct atatgacttg tggacagatt tttcacttcg acatttacca aagtctaagg
     4321 gtcgaaatcg tttgttagag ttggcctgtg gaaccggtat tcagtcagtt cgttttgctc
     4381 aagcaggttt tgatgtgact ggtttagact taagtcaaga catgctggct attgctaaaa
     4441 agcgtgcgca atctgctaaa aagaaaattg attttattca aggtaacatg ctggatttgt
     4501 cacaggtggg gcagtttgat tttgtaacct gctattcgga ttccatttgc tatatgcaag
     4561 acgaagttga tgttggcgat gtttttaaag aagtttatga tgttttagca aacgatggca
     4621 tctttatttt tgatgtgcat tcaacctatc aaacggatga gtgtttccct ggttattcct
     4681 atcatgagaa tgcagatgat tttgcaatgg tttgggatac ctatgctgac gaggctcctc
     4741 actctgttgt tcacgaactc actttcttta tccaagaaga cgatggacgt ttttctcgct
     4801 ttgatgaagt gcatgaggaa cgaacgtatg agttattgac ttatgatatt ttgttggaac
     4861 aagcagggtt caagtcattt aaggtctatg ctgattttga agataaggag ccaactaaga
     4921 ccagcaagcg ttggtttttt gtagcatata aatagaagaa aaaagtagcc tttttagcta
     4981 taggatttaa tgaattatct tgaatgttac ttatatgaaa agagttcatt aggcattaaa
     5041 tgctattatc aaagcttaga agtattggag acattacgat tgacactatg tctgaaggtt
     5101 gctaccaact ttaaagaaag cattttctat tatacgttgt gcttaagcat ttgatttaac
     5161 tcttattagc cctataaaag actatttagt acaatttgag gagattctat gactgttacc
     5221 ggaattatag ctgagtttaa tccgtttcac aatggacaca aatatttgct agaaacagca
     5281 gagggactaa aaattattgc tatgtcagga aattttatgc aaagaggaga gcctgctctt
     5341 attgataaat ggattcgatc agagatggct cttaaaaatg gtgcggatat tgttgtggaa
     5401 ttgccctttt tcgtttcagt tcaatcagca gactattttg ctcaaggagc tattgatatt
     5461 ctttgtcaat taggaattca gcaattagca tttgggacag aggatgtgat tgattatcaa
     5521 aaactgatta aggtctatga gaaaaaatca aagcaaatga ctgcctattt atcgacgctt
     5581 gaagatacac ttagctatcc acaaaaaaca caaaaaatgt gggaaatctt tgctggagtt
     5641 aaattttcag ggcaaacgcc aaatcatatt ttggggttaa gttacgcaaa ggctagcgct
     5701 ggaaagcata tacaactatg tcctatcaag cgtcaagggg ctgcctacca ttcgaaagac
     5761 aaaaatcatc tgctagcttc tgcaagtgcc attagacagc acctgaatga ttgggatttc
     5821 ataagtcatt cagtaccaaa tgcaggttta cttataaaca atccccatat gtcatgggac
     5881 cattattttt cattcttaaa atatcaaatt ctgaaccatt ctgatttaac tagtattttt
     5941 caggtcaatg atgaattggc aagtcgtatt aaaaaagcga taaaagttag tcaaaatatt
     6001 gaccacctag ttgacactgt tgctaccaaa cgctatacaa aagctcgtgt aagacgtatt
     6061 ttaacctaca ttttggttaa tgctaaggaa cctactttac ctaaaggaat tcatattcta
     6121 gggtttacct caaaaggaca agcacattta aaaaaattaa aaaaaagcag gcctctgatt
     6181 actagaattg gagcagaaac ttgggatgag atgacacaaa aagcagacag catctatcaa
     6241 cttgggcatc aggatatccc agaacaaagt tttggaagaa tacctattat aataaaaaat
     6301 gagaggctca attaaactct aattgagttt ttttgggggc ttgactctaa cattacgttg
     6361 at
//
LOCUS       DATUYR010000075         6154 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.075, whole genome shotgun sequence.
ACCESSION   DATUYR010000075 DATUYR010000000
VERSION     DATUYR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.075"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(167..1057)
                     /locus_tag="VCI33_001497"
     CDS             complement(167..1057)
                     /locus_tag="VCI33_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEP2832416.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFATKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            complement(1294..2958)
                     /locus_tag="VCI33_001498"
     CDS             complement(1294..2958)
                     /locus_tag="VCI33_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEP2832417.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKNKA
                     EVKEFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSDPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(2958..3323)
                     /locus_tag="VCI33_001499"
     CDS             complement(2958..3323)
                     /locus_tag="VCI33_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP2832418.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            complement(3475..3663)
                     /locus_tag="VCI33_001500"
     CDS             complement(3475..3663)
                     /locus_tag="VCI33_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEP2832419.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            complement(4045..4926)
                     /locus_tag="VCI33_001501"
     CDS             complement(4045..4926)
                     /locus_tag="VCI33_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEP2832420.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            complement(5133..5218)
                     /locus_tag="VCI33_001502"
     tRNA            complement(5133..5218)
                     /locus_tag="VCI33_001502"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5182..5184),aa:Leu,seq:aag)
     gene            complement(5272..6153)
                     /locus_tag="VCI33_001503"
     CDS             complement(5272..6153)
                     /locus_tag="VCI33_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP2832421.1"
                     /translation="MITLISVGASFYFFHVAQIREEKSFINNKKRSTNNPLYPAEQSF
                     DALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKEDMKPYAMLFHDLGYN
                     VLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQITLFGLSMGAATVMMA
                     SGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYEVSALSKIRAGFSYGE
                     ASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEILIVKGAKHAKSFETN
                     PEQYQKKIAAFLKKVEK"
BASE COUNT         1748 a         1349 c         1068 g         1989 t
ORIGIN      
        1 cttaacattt aatcaatact atgttgttaa tcatgaaaaa ggcttttttt actttcattt
       61 ctccaataaa agtaatgtat tcatttcgtc gtttgccact tcacaaggta tgatagcctt
      121 ctgctaaatc cagtattgag caacttttta gccaagcttt tacacgttat tttgttttaa
      181 gggcagataa aacttgtgta cggatatcct ctaccccact tggtgtattt ggtagaaaaa
      241 gggtttggtt tccttttgtc gcaaaggtat tgagggtatc caggtattgg ttggtcaaca
      301 aaatggacat gatttgttct tcgtttaagg aaatattcgc ttctttaagc tcttgaatag
      361 actccgccaa accatccaca atggccttac gctgttgggc aatccctacc ccgtgcaatc
      421 gatctttttc agcctctgct tctgctgctg tcacaatttt aattttatct gcgtttgcca
      481 actcttgagc agctacacgt ttgcgttgag ccgcattgat ttcattcatt gactgtttga
      541 cttcagcatc aggctccacc ttggtaatca aggttttgac aataatatat ccataggttg
      601 acatttcttc tgccacttga tgttgtactt ccaaggcaat ttcatctttt ttctcaaaca
      661 attcgtctaa ggtcaacttc ggcacggatg accgcaaggc atcttcaata taagacttga
      721 tttgagattc aggtttcatt aatttgtagt aagcatctgt tacattttgt tcgttaacac
      781 gatactgagt ggcaacattc aaagttacaa acacattgtc cttggttttg gtttcaacga
      841 tgatttctga ctgtaaaaga cgcagctgca cacgcgcagc aattttgtca atcccaaacg
      901 gaaggcggac atgaatacca ctcgtggctg ttttttggta cctaccaaaa cgttccacga
      961 tggcaacaga ttgttgacga acaacataaa gggtactggc aacaatagct aggattacaa
     1021 taaccccaaa agcaataaag ataaatggtc ctaacataaa atctcctcct tgtcatatct
     1081 tagaactgaa ccgttttcag ttcttgactt attgttataa tacaattata atgtttttct
     1141 aggttttgtc aaactaaatc gtaaccatta gctttgtgat ggattaccct cattttttag
     1201 gggcttacca tcactaaaac cactcattac gacatcaata tagacaaaaa gagtcagacc
     1261 tttgcctaac tcctcttttt ttattgtatt ctattattct acactcatga gatatgggta
     1321 aacaggctga tctccttgat ggatctcaac ttccacatct tcgtaagtct ctcctaaata
     1381 cccagcaagt tcttctgcta agtcttgatc accttcttca ccaacaaaga tagtcacaat
     1441 ctcgctatct tcgtcaatca tcttttcaaa ggcagctttt agagttgctt ccatgtctgg
     1501 gtctgaaacg atgattttac catcaaccat tcccaagaaa tcattttcat ggatttctaa
     1561 accatcaatg gtggtgtcac ggactgccaa agtaacgctt ccactgacca cgtcagaaag
     1621 actagttgac atatctgcta cattgtcctc taaagatttt gaaggatcaa aggccaacaa
     1681 gctggtaaat ccttgcggaa cagtacgtgt tgcaacaaca gcagccggga tatccaccac
     1741 ttctgcagca gattgggcag ccataaagat atttttattg tttggcaaga taatgacttg
     1801 tttggcatta actgcttcaa tagctttaac aatatcttct gtagatggat tcatggtttg
     1861 accaccagaa atcacgtaat caacaccttg cgccttgaaa atttcagata aaccttctcc
     1921 agctactact gcaatcaagc caaattcttt tacttcagcc ttgttttttt cgacatcagt
     1981 cttttgaacc tgtgcttcat gctggttacg catattgtcc actttaatct tgatgagcga
     2041 accatattta agaccttctt gcatcacaag tccggggtct tcagtatgga cgtgaacttt
     2101 aacaatctca tcatcattga ccactaacag cgagtcacca aggccactaa ggtagccttg
     2161 aaactcatca tagttgaatt ctttcacata ggttgggcct tgtttaaggg caaccatgat
     2221 ttcagtacaa taaccgtagg taatgtcttc agtagccaca tgaccaacaa cggatttgtg
     2281 atgctcagca ttaatcattt cagacatgtt agcaggagtc gctttaaagt ctgctgaagt
     2341 cacatagtcg ccattcaaag cagataggaa accttcatag ataaagacaa gaccttgacc
     2401 gcctgaatca accacaccaa cttctttaag gactggtaat aaatcaggtg tcttagcaag
     2461 ggcacctttg gcaccgtcta aagcagcttg catgacttcc acggcgtcgt ctgtaagatc
     2521 cgcttttttt aaggcagcgg ttgctgctcc acgagatact gtcaagattg tcccttcaac
     2581 aggtttcatc actgctttgt aggcaacttc tacacccact tgaaaggctt gtgccaaatc
     2641 tttacctgtc agctcatctt ttcccttaat actttgacca aaaccacgaa aaagttgtga
     2701 agtaataact ccagaatttc cacgcgctcc catcaaaaga ccttttgata acatttgtcc
     2761 aacttctccc acagttgacg caggtttatc agcaacttcc ttggcaccat tgtccatagt
     2821 catactcatg tttgttcctg tatcaccatc tggaacagga aaaacattta aggaattaac
     2881 atattctgct tgtttgccaa ggcgagtcgc tgccgcttgg accatttctt ggaataagct
     2941 tgttgtaata tttgacacta attttctcct acaaccttaa tattttgcac gtaaacattg
     3001 accatatcag cagtgagacc aagctgactt tcaaggttaa atttcacgcg ttcttggata
     3061 tttttagaga cttcactgat tttcacacca taactcatca cagtgtagac atcaacagaa
     3121 atacccagat ctgttgattt tactacaaca cccttagcgt agttttcttt gcgaagtagt
     3181 gattgaaaat tgtcttttat tgcacttttg ctggccattc caacaacacc aaaaatttcc
     3241 gttgctgatc ctcccacgac agttgcaatc acatcgtcgg atagctcgat tagaccatct
     3301 tttgtattaa tttttacagt catagttact acctcaaaaa gtttttatca tcctatttta
     3361 tcatatttta gaagtaatgt aaaagagata gaatagttaa cgatagcgtt tattaccctg
     3421 actagcttat ggatagaaat aagctaataa aaaaagccct aaggcttatt tctattagat
     3481 gcgttctact ttaccagatt taagcgcacg agcagaagcc caaacttttt taggtttacc
     3541 atcaacaagg atcgtaactt tttgaaggtt tggtttaact gtacgtttcg tttggttcat
     3601 cgcgtgtgaa cggttgtttc cagaaacggt tttacgtcct gtaaaataac atactttagc
     3661 cattttgatc tgtttcctcc tacgtagaat atgtttacgg atgtgctagc accacatacc
     3721 tatctattct atcatagggt gcttaatttg acaaggtatt tcccattttc tttttcaaca
     3781 attttacgga attactatta caatagtcta cgtactcatt tttcactaca ttttttagct
     3841 aaaacatatt aacattccgc ttaacctctt taacaataac aaaaagactt accatagata
     3901 gcagggtcca gattttaata ctctaaatta tgctttgttt gttcacaatc aaagattgtt
     3961 tcacagaaac tggtatccat tttagggtgt caattgtatc taaaaaagcc ctcaagaggg
     4021 ctttagggta tttaatactc taaattatgc tttgttagct gatccgaaaa catcgatacg
     4081 ttcttcaaca gcaccttgaa cagctttcat acctggagcc aagaatttac gtgggtcaaa
     4141 taatttttta ccatcatatt ctgcttcgtt agcttcgtag ttacgagcaa attcacgagt
     4201 tgcgttagag aaagcgattt gactttcagt gttaacgtta actttagcaa caccaagacg
     4261 gattgcttct ttgatttgat cgtcaggaat accagaacca ccgtgcaata cgattgggaa
     4321 acctggtaca gctgctgtta atttttctaa gtggtcaaga gcaagacctt cccagttttc
     4381 tgggtatgga ccgtggatgt taccgatacc agctgccaag aagtcaattc cagtttcaac
     4441 cattgcttta gcatcttcga ttggagcgag ctcaccttta ccgatgatac cgtcttcttc
     4501 accaccgatt gtaccaactt cagcttcaac tgaaacgcct ttagcatgtg caattttaac
     4561 aacttctgca gttttagcaa ggttttcttc aactggaagg tgtgaaccgt caaacatgat
     4621 tgaagtgtaa cctacttcga tacactcaag agcatcttcg tagtgaccat gatcaaggtg
     4681 gatagcaaca ggaacagtga tacccattga ctcaacaagg ttagtaataa gagattgaca
     4741 tactttataa ccacccatgt attttgctgc acccattgat gtttggataa gaactggagc
     4801 ttgtttagct tctgctgcac gcaagattgc ttgagtccac tctaagttgt ttgtgttaaa
     4861 tccaccaaca gcatatccgt tttcacgagc tgcttggaca aatttttctg ctgaaacgat
     4921 tgccatttgt aaggcctcct cttatttttc gggataactc cccgtttaca ttgttcattc
     4981 tagcacaatt ttccataaat ttctagtctt tcacaagctt aaagcgtttt ccaaaagcag
     5041 attatcaaaa gttttctcca gtaaatctat gccttcttat ccttcattta accaaaaaaa
     5101 agagacgaat caatgattca cctcttacat tatgcggaga gtgggacttg aacccacacg
     5161 acctaaagcg gtcacaggat ccttagtcct gcgcgtctgc caattccgcc atccccgcta
     5221 ctcatcagta acataaatta ttatagcaac tgtgagttta actgtcaata cttatttctc
     5281 aacttttttc aaaaaagcgg caattttttt ctggtattgt tctgggtttg tttcaaagga
     5341 tttggcgtgt tttgcccctt taacaatcaa gatttcctta ggacctttcg tggccttata
     5401 attgtcataa accatttttg taggaacaaa atcatcctta tcaccgtgga taaataaagt
     5461 tggacgttta tttttagcca gctgtttcac tgagctcgct tctccgtaac taaaacctgc
     5521 tcgaatctta gataaggcag agacttcata gagtaaagga aaggcaggca agttgtacat
     5581 agccttggcc tgaaacttca attcgtccca aacactggta taaccgcaat cttcgatgag
     5641 ggaggtgact tgcgcaggca atcgctcacc acttgccatc attactgttg cagcacccat
     5701 agataagcca aagagtgtga tttggctttc agggttttcc ttaatcagtt ggtctgtcca
     5761 agccatgaca ttaaggcggt cattccagcc ataaccaatc aagttccctt cactttcccc
     5821 atgggcctca ttgtctggca ttaagacatt atagcccaaa tcatgaaaaa gcatggcata
     5881 tggcttcata tcttctttgt cattcgtaaa accatgaaca acaatagctg tcttttttgt
     5941 tttttgagca gctggtaagt accaccccac ttgttttaac ccacgatttg ttagttgacg
     6001 tttttcgtaa ggtaaagcgt caaaagactg ttcagctggg tataatggat tatttgtact
     6061 ccgtttctta ttattaataa acgatttctc ttctcttatt tgtgcaacat gaaagaaata
     6121 aaaactagca cctacgctaa taagagttat caaa
//
LOCUS       DATUYR010000076         6131 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.076, whole genome shotgun sequence.
ACCESSION   DATUYR010000076 DATUYR010000000
VERSION     DATUYR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.076"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            101..781
                     /locus_tag="VCI33_001504"
     CDS             101..781
                     /locus_tag="VCI33_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP2832422.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(902..1549)
                     /locus_tag="VCI33_001505"
     CDS             complement(902..1549)
                     /locus_tag="VCI33_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEP2832423.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(1552..2211)
                     /locus_tag="VCI33_001506"
     CDS             complement(1552..2211)
                     /locus_tag="VCI33_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEP2832424.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVTLIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQVREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(2223..3407)
                     /locus_tag="VCI33_001507"
     CDS             complement(2223..3407)
                     /locus_tag="VCI33_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP2832425.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            3528..4439
                     /locus_tag="VCI33_001508"
     CDS             3528..4439
                     /locus_tag="VCI33_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP2832426.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEAKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFMNQRF"
     gene            complement(4509..5915)
                     /locus_tag="VCI33_001509"
     CDS             complement(4509..5915)
                     /locus_tag="VCI33_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEP2832427.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT         1970 a         1333 c         1003 g         1825 t
ORIGIN      
        1 ttttgataaa aaattattat taaggtgatt ggcaattgtt ttttagtcta aaatgtatta
       61 ctatatttgt aaataagaag atagatatag gaagtttgac atggataaag aaacgctaaa
      121 ctactggaaa acggttatca catttcttca cgatgtctta ggtgataact atgagattat
      181 tttacatgtg attgataaaa atgatatcta tattggagaa ctcgttaata gtcatatcag
      241 tggacggtct aaacagtctc ctctcaccac ctttgctctt gatttgatta ccaataaagt
      301 ctataaggaa aaagattttg tgaccaatta caaagcaatc gttagtcctc agcacaaaga
      361 agttcgggga tctacttttt ttattaaaga taaaaagggt aatctcgagg gaatgctatg
      421 tattaactta gatatttcag cctatcaagg agtagctagg gacctcttaa aacttgtcaa
      481 tctcaattta gaacatttca tcccaactgc taaagaacct aaaacagtca caccccagcc
      541 agaagaagct gttgaaattt taacctctaa catccaagat attatcggtc aaattattga
      601 tccctcgtta ctgagacaca atgtacatct tagccaagat gtcaaaatag acattgttgc
      661 caagctttat gaaaaaggag tatttcaact aaaaggagcc gtctcaaaag tcgctgatat
      721 tctatgtatt tccgaaccaa gtgtttaccg ctaccttaaa aaaattgaag ccgatcaata
      781 accaaatcac cataaaacgc cagttaaaag tatttcagat aagcatcaca atctctgata
      841 taccgatgac tgacgttttt ttactcaata tgcctttatg gcaagaaaag gctactagtc
      901 attaaccttc agctatgctt agaggtacac ctgtcgcagc ttgaacctca tcaaaagtaa
      961 acccttcact gatttcaatt acttggatac catctggcgt tactttaaac acacccattt
     1021 ctgtgataat aaggtcaacc acaccttttg ctgttaaagg taaggtacat tcttttagga
     1081 gcttatgctt tcctttattg gtatgctcca tagctacgat gactttttta gcaccaacta
     1141 ataaatccat cgctcctccc ataccaggaa ccattttgcc tggaatcagg taattggcga
     1201 tatctccatt ttctgcaatt tctaaagcac ccaagacagt caagtcaaca tgaccaccac
     1261 gtataatgcc aaaagaatct gcactattaa agaaagcggc tcctggataa accgtcacag
     1321 gctgtcctcc tgcattaacg atagtcgaat tcggatcatc tgacttagga cctagtccaa
     1381 taaacccatt ttcagattga aaatacactg tcatttcttc aggcacaaaa tttgctactt
     1441 tggttggtag accaatacct aaatttacca aagtaccatc ttctaattct ttggccacac
     1501 gctttgcaat acgattttga atctcttctt tacttaatgc aaccgccata actatactcc
     1561 ttctttaaca atgtaatcta caaaaatgtg tggtgtatga acgcattctg gttgtatggt
     1621 gccgactggt actatttctc gcacctgaac aattgttgtt tttgcacaag ctgccatcaa
     1681 ttgattaaaa ttattttcag aaccactata ctgtaaattt cccatttcat cagctttatt
     1741 agcaaatatc aaggtgacat ctgcttcaaa tgggtattct aagagatagg tttttccctt
     1801 gatggttttt gtttccttgc cttctgccac taaggttcct acccctgttg gcgtgtaaaa
     1861 gccaccaatg ccataagctc cgatgcgaat tttttcggcg aaggttcctt gaggaatgag
     1921 ctcaatcgtc gtttcaccgg cagtcatttg gcgcccagct tctttattta atccaatatg
     1981 tgttgcataa atggttttga attgatgatt agccaccatt tttccaacac cgcgatcaac
     2041 aaaaccagca tcattacaga tcaaggttaa atcttttgtg cctttgtcaa ccagtgcatc
     2101 aattaacgct tctggtgttc cattagccat aaaaccaccg accataatcg tgtcaccatc
     2161 tttaatatga ctgatagctt ctgctattgc tattctttta ttctctttca tacggttacc
     2221 ttctatcttg ttacgatgat actttgacct tggccaccac caatacaaag cgttgctaag
     2281 ccatgtctca catcacgctt agccatttct gctaataagg tgactaaaat acgagcacct
     2341 gatgctccaa tcgggtgacc caaagcaata gctcctccat tgacattaac tttttcattg
     2401 tcaaaaccaa ggtctcgtga aaccgctaaa gcctgagcag caaaagcttc gtttgcttca
     2461 atcaaatcaa tgtcatcaat tgtcaactga gcctttgcaa gagccttttt cgtagcaggt
     2521 attggtccgc agcccataat acttgggtct acacctgcac ttgcataact agtgatttta
     2581 gctaaaatag gaagccctaa ttcttcagct ttttctttac tcatcaataa aattgctgct
     2641 gctccatcat tgattcctga agcattacca gcagttactg tcccctcatc tttgataaaa
     2701 gcagggcgta actttgctaa cttatccact gtcgttccaa atttagggta ttcatcttga
     2761 tcaacgagta aaggttcacc tttacgctga ggaatagtga caggtgcaat ctcttctttg
     2821 aaacgacctt tttctatggc aagttgggct ttacgttggc tatcagctgc aaaggcatct
     2881 tgttcttctc tggtcagtcc atactgttgc acaatatttt cggctgttat acccatgtga
     2941 taacctgcaa aagcatcact caacccatct ttaagcatgg tatctactac tttactatct
     3001 cccatacgag atccccaacg ttggccttgt aaaacgtaag gtgcttgact catgttttcg
     3061 gtaccaccga ctaaaacaat atcagcgttg cccgtttgaa tcttttgagc acctaactca
     3121 actgctttta agccagaacc acagaccata tcaatcgtaa aagcaggggt atcataagaa
     3181 agccccgcat ttagcgctac ttgccgagca acattttgac ccagacctgc atgtaacaca
     3241 ttcccaaaga tcacttcatc aatggcatca cttttaagac cggtatcagc taaaattttt
     3301 gtaacaactg tcacccccaa atctacggct gataaagatt taaagactcc tccaaaattt
     3361 cctatcgggg tacgataagc agatgtgatc accacttctt tagtcataga aacttctcct
     3421 ttttcattat tgaaacgctt acatttttat tatataatgt gaactataat aaacaaatat
     3481 cgttttttta tatatgctat aactaaacgt tatcgaaaga ggaaaatatg aatatctcaa
     3541 gcttggctta ttttattgag attgttaatt gtgactttaa cttaaccaaa gcagcccaaa
     3601 aaagtcatat ttcgcaaccc gcccttagta gttatatcaa gactatcgaa aaagaagaag
     3661 gagtcgtttt attccagcgt caaaaagggc gtctagttgg actaacctca gtcggcgaac
     3721 gcttttttga aaatgctaag gtggttatcg catcacatga gcaattaatg gctgatttgc
     3781 ggagtgaatc caaaaaatac aaaggagaaa tttcaatcgg tattcctcaa ctcatctctg
     3841 gcattgtttt ttctgagctc attccaaaac tcatcgaaga aaatcccgaa gctaaatttc
     3901 atattgtaga aacgggtgct tatgaactgc aaaaaaaact gtttctaaaa gaagtcgatt
     3961 ttgcgatttt attaacaccc actacactag atgctactat tgctgatatc gacattctcc
     4021 taagagatca actctgcctt tatatgagtc acaaaacctt tcaagagacg tttgatagta
     4081 atcctaacat ctctatcaaa gcattacatc gtcaaaactt agtaactttt gataaaacct
     4141 ttatgattaa tcatcagctt accaccttat ttgaagaagc aaatagtctt cctgtagtca
     4201 aatgttattc tgcctcgtgg gattttttat taaattgcac gcgctattcc tcttacctaa
     4261 caatcctacc aaggcctatt actcattttg ctcatatgga cggtttagtg gaggtccaac
     4321 tgaccgaaca tccaaaatgg gaagttgttc tagcctctct caagcacaat aaaacaagcc
     4381 atttaaaaca ttatatcaag cacaccatct tagattattt catgaaccag cgcttctaat
     4441 tgcttgactc agaccttaaa aaaaaacgcc tgaaggaaac aatcccaagc gtttcatgta
     4501 gttatttcct aaaatacaaa taggtaaatg atggcaatac ctgtgaccac caagacacaa
     4561 ggaatagtat aagccacaat ttgattgaat tttagtttga agtttgagag ataagataag
     4621 gcccaaaaag gaactatcaa gtttgttgtg gcatctccta attgataagc ctgcaaaatc
     4681 ttacctagat cattaccgac ttcgatagtt gcagggacga tatatggagc ttcaatcaca
     4741 aattttgagc ctcctgatgg taccgcaata ttcagcaaag ccgagtaaag ataagcaaag
     4801 accggccatg ttgtcccatt tgaaatagcg acaaagaaat gggaaatctc tagtcctaga
     4861 cctgtacttt gaatgattcc aaaaatacca gcgtaaaatg gaaactgaag caccaagccc
     4921 caagatgaca tgaccccatc tttgaataag gaaccataat attctggtcc ttgttgacca
     4981 caaagaagca accctaagcc taaaaataaa aagttaaaac tattgagatc tagaccagac
     5041 aagcccacca ccgaaaaatg ataaaggcta tagctaacaa tggccccacc aacaagatac
     5101 atcactaagc gagatgcgtt catcttttca gcaggtgtgt ttcttgcaat aacaacatgt
     5161 gaagcggtat caagactatg gtgatagatc tcatcatcaa gttccatgat tttagtttca
     5221 tcttttgggt gcgccaataa agcaaagcaa agcggtacca gcatacacac cactaaaaga
     5281 ctgataaatg gaagattcaa aactgattct gtcagaggaa cactttcagg aacaacctgt
     5341 ttataagcat ctgcgacact atttcgcaaa taatctggag tagccgaata aagcaccgca
     5401 gcaccagata aaccagaagt agctggtaaa aaggtaaaaa ataaagtagc aacaaacaaa
     5461 ggtgtatgca ccttaatccc tttttgccta gcctgaacaa gcaactcttt cccaaacaca
     5521 atggcaacca tcataccaag accccagtgt aaaaagccaa agatagaacc tactacaata
     5581 gaaaaaataa aaatctgagt cctggtttga ggcagtttgg caagggactt aaacatttta
     5641 tgagcaggtg gagaactcgc tacagcattc cctgtcgcaa gtaaaatagt catctgcatg
     5701 gcaaaggaca acagccccca aaaaccattt acccagccat agataatccc gcccgtcttt
     5761 tccgtaccaa taaagggact atccgtcatc aacacagaca tagtaacgac taaaaaggtt
     5821 aaaataaaac agataaaaag cgattctggc atccatttca taaagccatc aatatagcga
     5881 tccataaacc tttttttggt tttgatgtta ttcatgttgt caattccttt cacattgtcc
     5941 acaagcactt ttgttctttt gatcagtaac ctatcgataa tactaataag cgtttcttta
     6001 gttcatttag ttatcaataa gtttaaaccc acaaaaaaca cttttgttcc ctttttatta
     6061 tgacaacgtt ttcatagaca aacaactatg atttttttat tgcttccata acccttattt
     6121 atagcaaaaa g
//
LOCUS       DATUYR010000077         5639 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.077, whole genome shotgun sequence.
ACCESSION   DATUYR010000077 DATUYR010000000
VERSION     DATUYR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.077"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..179)
                     /locus_tag="VCI33_001510"
     CDS             complement(<1..179)
                     /locus_tag="VCI33_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEP2832428.1"
                     /translation="MGLLDLIGRKRARDKPQNSYEGQDFFYLFGRTTSGENVDEFKGI
                     KVEFIQLSNKNIFDV"
     gene            complement(284..556)
                     /locus_tag="VCI33_001511"
                     /pseudo
     CDS             complement(284..556)
                     /locus_tag="VCI33_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(575..880)
                     /locus_tag="VCI33_001512"
     CDS             complement(575..880)
                     /locus_tag="VCI33_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP2832429.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQEM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(880..1182)
                     /locus_tag="VCI33_001513"
     CDS             complement(880..1182)
                     /locus_tag="VCI33_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEP2832430.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLIFSFEDAKTGNRKDVREFLSQLKENA"
     gene            complement(1237..1413)
                     /locus_tag="VCI33_001514"
     CDS             complement(1237..1413)
                     /locus_tag="VCI33_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEP2832431.1"
                     /translation="MGQNVVMRQAPGGNIKPDKEKSVEKIDGIVGLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            1429..2322
                     /locus_tag="VCI33_001515"
     CDS             1429..2322
                     /locus_tag="VCI33_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832432.1"
                     /translation="MTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQADTAIPRYLL
                     VTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHKATL
                     QDIYFCQTIVSYFIQKCEVLE"
     gene            2322..2546
                     /locus_tag="VCI33_001516"
     CDS             2322..2546
                     /locus_tag="VCI33_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832433.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            2537..4195
                     /locus_tag="VCI33_001517"
     CDS             2537..4195
                     /locus_tag="VCI33_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEP2832434.1"
                     /translation="MLIEMWSPVFKNHGEIRESICFHYGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSRDTANPDIILVCDKSYSP
                     TGETMDLGIFLNKLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTHQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETILDSEFSTERESLIAEIDEL
                     ESQLTTLNQELQELGNIPNLSSEYLESYSKLTATINALKEQNEAYLKESGLSKEKSEA
                     DADLKRSTEDILLDLEVKINSKMREFNNILYPDIRKAPQINLNAHNSYSFYTPDDDGT
                     GTKFKGMIVFDLVMLYLTNLPALTHDSLLLSNVSYQATEALLKLYDQSRSLNKQVFLA
                     FDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            4185..4394
                     /locus_tag="VCI33_001518"
     CDS             4185..4394
                     /locus_tag="VCI33_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2832435.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
     gene            complement(4497..5636)
                     /locus_tag="VCI33_001519"
     CDS             complement(4497..5636)
                     /locus_tag="VCI33_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP2832436.1"
                     /translation="MFDFANNTFLAGLNPASLSLVAVYQSLESVIGVLFNLFGGVIAD
                     SFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMSAFSSPSYKAFTK
                     EIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLDGLSFLIAALLIS
                     FILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSALVNFFLAAYNLLL
                     PYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLILFLSLSGLMLML
                     APPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLGRVFGIIFTITIL
                     FMPIGTGFFSVALNPNNSFNLFIIGSCITILSLVFRILFKKYNIR"
BASE COUNT         1982 a         1093 c          909 g         1655 t
ORIGIN      
        1 aaaacatcaa atatattttt gttagacagt tgaataaatt ctactttaat tcctttaaac
       61 tcatccacat tctcgccact agtcgtgcga ccaaagaggt agaaaaagtc ctgaccttca
      121 taactgtttt gaggcttatc tctagccctt tttcttccaa ttaaatcaag tagtcccata
      181 agccctcctt attttggggt acaaaaaaag cacctccttt gaagtgcttt cggtatattt
      241 ctttcaaagt aatcaacagt ctttcttaca aaacctagta ttctcattct gtcaattcct
      301 taaatgcgtc aagatgtcgt gataatactg aagtcgcaat ctcatctaac cttgcttgtt
      361 caacaactgt tgtcatctgc tcatcactgc ttaaactttg gtgatccgct gatagtttgc
      421 tatcagatgg acttaatacc aaagttccat cagttgattt ataaaggtta aactcctaat
      481 tctcttgaat gccaaatggg tcaggtatcg gaagatagat gtcatcacca atttgtatcg
      541 tctttacgag ttccatacat cactctccct tactttacca attattcata tgacgtgatc
      601 catagacgac cgcaacaatt ataacttcat cttccagaac atggtataca atgcgatatt
      661 ttttgacaat cagttgtctg aaggtatagc cttttccaat cagatcctca atgatggcac
      721 aacgttcagg aaaaatggat agtgataaca tttcttggga taacctctca agaagattat
      781 ccgctgcctg tggtgcacag agttcatcac gaacatagtg atagatgctc agcaaatctg
      841 ctttagcatc atccgacatg gtgacctgat attctttcat taagcatttt ccttcaattg
      901 agatagaaat tctctgacat ccttgcggtt gccagttttc gcatcttcaa agctgaagat
      961 aagcttatca taaaacttct cctgactcat gtaatcaaca ttaactcgtt gaggcgcttc
     1021 aggtagagaa acatcaaatg ggataccccc agtcaaaata atctgattca aaaacatatc
     1081 aatcgcagtt gacataggaa tacctaagcg tttcaaaata tcatctgccg cactttttac
     1141 tgaatcatca actcgtaaat tcaaagttcc cgttttagcc atggttatcc tccttttttg
     1201 taacgacatt gtatcacgtt tttagagttg actcaactaa aaactcaata tccctcgctc
     1261 atcatagacg ctagcttcat caccttggcg gcgaatgcaa cggtcaagcc ccaggataag
     1321 acctacaata ccgtcaatct tctcaacaga tttttcctta tctggcttga tgttgcctcc
     1381 aggggcttgt cgcatcacca cgttttgtcc catccattta aggactggat gaccaccatg
     1441 ttagattttc ccttccatca tgagtttata gagttccttg gacggtggac tcatatcctt
     1501 ataactctgc tcaaaaggca ccatggtcaa gccgacaccg caattccaag gtatcttcta
     1561 gtaaccataa gagagaagac agggattgta aaatcaacca ctgtgcaaaa tactgcgttc
     1621 actacccagt cgaatcagct caaaaaacaa tacgggcagt tcttgttaac agaatctaat
     1681 aactgttgtg catgcccagg ctgtgataga ccactcattc taaccagtgg caacttagca
     1741 gcagaaaatt acgaggtttc agccattgaa aaagataagg acgctgaacc actgaatctg
     1801 atagccctct gcccagattg ttttttaacc tatcaagcag atagtcgcaa aaaaattgta
     1861 acagctctaa aaaatgttaa aaagattcta gtctctgcac ataacagcca acaagcgatt
     1921 tctgatatga agttagacag tggaattgtt tcagttttga ctagtctcaa taaactaaaa
     1981 tgtgatgaat atgatatctc ctatgactcg aagcaattga ctgataagat atcacctgag
     2041 aacaatcgta cgctttatca attggttaaa aacccagtta ttgataacta cttaaccatt
     2101 caaaaaatca tcgtaaattt ggacaaacaa ggaaaaattg attacgagga aatccaatat
     2161 caaacgcgat cgatgtataa aaagttaaaa gcagccaagc ataataatct ggctattttc
     2221 aatactattt cggaaaaact ccataaagct acactacagg acatctactt ctgtcagacg
     2281 attgtctctt actttatcca aaaatgcgag gtacttgaat aatgcaatta ccaaataaac
     2341 tttactccta tcaaaaaagc acccttgctt atttgcctag agtgctaaat gaaatcaaaa
     2401 gtggaaactc aaacgtcaaa gatatctttc acgctatctc agaagagttg gatgatccta
     2461 cagatttctt atctataatg gattgcttgt atgcattgaa cgctattgaa atgtctaatg
     2521 aaggagaagt aaaattatgc ttatagaaat gtggtctcca gtatttaaga atcacggaga
     2581 aattcgggaa tcaatctgct tccactatgg gttaaacgtg attatgggga tggatttagc
     2641 tgataactct atcggcaaat cctcctcact attagccatt gatttcatct ttggaggtaa
     2701 tagctatcta aaatcaattg cggttaaaaa attaggtgat catcctatct atttctgctt
     2761 tcaatttgag aaaaagtttt atttttcaag agatacggcc aacccagata tcattctagt
     2821 gtgtgacaag agctactctc caaccggtga aacaatggac ctaggaattt ttcttaacaa
     2881 acttaagaaa cgatatcatt tagattctcc agagctatca ttccgattag ctatgagtgg
     2941 cttctttagg attgcaggaa aaagtaatca aaataccgat ttccctttac aagtttattc
     3001 gagccaaaaa tcttctgaat ctattactac tctcatccaa ctgtttaatt tgtatgataa
     3061 cattgcacga tacaaagaac gtctgaaaga taagagcgac cagttaacaa cttttcgtaa
     3121 tgctcgaaag tataccttca tctccaacct ggttggcggt aaaaagcaat ttgaagcaaa
     3181 tgtctcagaa ataaaaagat tggaatatga tttagcacat ctccaagaca ctcatcaaga
     3241 taaaattgac tctgatgata ttgagaaaaa tcaacaaaaa ctccagctta gaaatactaa
     3301 gctggagtta gaaagcagtc ttcgggacaa gcaaagacgt cttaagctat tggacatcag
     3361 tatcgaattt ggcttatatc ctactgaatc tgacttaaca gagcttcaac aatacttccc
     3421 agatactaat ctcaaaaaat tatatgaagt tgaagcttac cacaaaaaat tggaaactat
     3481 tttagattcc gaattctcaa ctgaacgtga atccctaata gctgagatcg acgaattaga
     3541 gagtcaatta acaactctaa accaagaact tcaagaacta ggaaacatcc caaatctttc
     3601 aagtgagtac ttagaaagct actctaaact aacagctaca atcaatgcac taaaagaaca
     3661 aaacgaggcc tacttaaagg agtctggtct atccaaagaa aaatcagaag cggacgctga
     3721 cttgaaacgc agtacagaag atatccttct agatctagaa gtaaaaatca acagtaagat
     3781 gcgtgaattc aataacattc tatatccaga tattcgcaaa gctcctcaaa ttaacctcaa
     3841 tgctcacaat agctactcct tctatacacc agatgatgat ggtacaggaa ctaagtttaa
     3901 agggatgatt gtattcgact tagtaatgct ctaccttact aatctgccag cactcacaca
     3961 cgattcatta ctcttgagta atgttagcta ccaagcgact gaagcacttt tgaagctgta
     4021 tgaccagtca aggtcattga acaaacaagt ctttttagcc ttcgataaag ccagctctta
     4081 ttctccagat gctaatcaac tcttgtcaga aaatacggta ttgagactct ctagtaacgg
     4141 aaatgaactc tacggtattt catggaacaa aggagaaaac tcagatgaga tttagttaca
     4201 ataaattatg gaaacttctt attgataaag gttggaccaa aacaaagctc aggcaagaag
     4261 ctgggattag ttcttccacg atcgcaaaat tgggaaaagg ggaaaatatc actactgata
     4321 ttttattgga aatttgtatt gcacttgatt gtaagattga agatatagta gaaattgtag
     4381 ataataatat ctagaaataa aaaaagcact aagttgataa gatgtgtaag tcagatcaac
     4441 ttagtgcctt tactttgaaa aaaaataatt ttctcaataa tacttagtac taactgttaa
     4501 cgaatattat actttttaaa taatattcta aatactaatg acaatatagt aatgcacgaa
     4561 ccaataataa aaagattaaa gctattgtta ggatttaatg ccaccgagaa aaagccagtc
     4621 ccaataggca taaaaagaat agttattgta aagataatac caaacactct ccctaaaaaa
     4681 tcgttatcaa catctttctg gacaagagaa aaaaattgaa tattaaagat ggaaagaaat
     4741 aaactgaata aagctggtga caaagctaaa ataattgcat tatgaaacat aatataaaat
     4801 gggggagcta acattaacat cagtcctgat aaagatagaa acaatatcaa tctcatactt
     4861 gataattctt tattcacaaa accacttaat attgccccaa taaatccacc aattgcttct
     4921 gctgttaaaa aagtgccata aagtccagtt gaaatttctc caaacatttg attactatag
     4981 ggcaacaata aattataagc ggctagaaaa aaattaacaa gtgcagaaag aacggtaata
     5041 ataaagatgg acttatggct gtaaacatat ttaaatccta tttttaaatc gttaaatatt
     5101 tctctgattg tcaccttctc ctttatcacc acttcgtcat taacaggtaa aataaaggaa
     5161 attagtaaag cagctattag aaatgatagt ccatccaata gtaatacacc gtgtatacca
     5221 agtagcttat ataagaaaat tgctaccata ggaactgtta ctttgattac agtacttgtt
     5281 gtctctagca atgaattaag ttgtgatata ctatcttttt taacaatttc ttttgtaaat
     5341 gctttatagg atggactaga aaaagcactc ataaatgcca agataacatt agttagtaca
     5401 atggcataaa ccaaccattg ctcttttgtt aggaacgaga gtactaagca agctgtgcca
     5461 cataagatat ttgtagtaat aataattttt ttccgcttga aactgtctgc aatgactcca
     5521 ccaaataaat taaatagaac acctatgaca ctttctagag actgatatac tgcaactaat
     5581 gatagagacg ctggattaag cccagcaaga aaagtattgt tagcaaaatc aaacataac
//
LOCUS       DATUYR010000078         5507 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.078, whole genome shotgun sequence.
ACCESSION   DATUYR010000078 DATUYR010000000
VERSION     DATUYR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.078"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1225
                     /locus_tag="VCI33_001520"
     CDS             <1..1225
                     /locus_tag="VCI33_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP2832437.1"
                     /translation="NGLTVYFIKKIGFLEKTAMLTVGFGSLDNKLTVDDESRDAPAGI
                     AHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQTSFFFSTASKFQENLELLQYFV
                     LSANITDESVSREKKIIGQEIDMYQDDADYRAYSGILQNLFPKTSLANDIAGSKASIQ
                     KITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLAIQRFQTTLSYPDRKRVTVDPLH
                     YYPVIKSSSVDMDVTIAKLVVGFRGYLTLTQHSLLTYRIALKLFLSMLIGWTSKIYHT
                     LYEDGKIDDSFDVDVEIHHHFQFVLISLDTPEPIAMSNYIRQKLATIKISKEFTNEHL
                     NLLKKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKETYFDIPKIIERLTLKDVVTIGKA
                     FFEKADASDFTVFPK"
     gene            1275..2300
                     /locus_tag="VCI33_001521"
     CDS             1275..2300
                     /locus_tag="VCI33_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2832438.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIAILGTVAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            2314..2856
                     /gene="pgsA"
                     /locus_tag="VCI33_001522"
     CDS             2314..2856
                     /gene="pgsA"
                     /locus_tag="VCI33_001522"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEP2832439.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            2858..3697
                     /locus_tag="VCI33_001523"
     CDS             2858..3697
                     /locus_tag="VCI33_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP2832440.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            3673..4515
                     /locus_tag="VCI33_001524"
     CDS             3673..4515
                     /locus_tag="VCI33_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP2832441.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELKEV
                     LKHG"
     gene            4508..5308
                     /locus_tag="VCI33_001525"
     CDS             4508..5308
                     /locus_tag="VCI33_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP2832442.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
BASE COUNT         1792 a          880 c         1024 g         1811 t
ORIGIN      
        1 aaatggttta actgtttatt ttataaaaaa aatagggttt ttagaaaaaa cagctatgtt
       61 gacagtaggt ttcggttctc ttgacaataa gttgacagtc gatgacgaat cacgagatgc
      121 tccagcaggt attgcacact ttttagaaca taaactcttt gaagatgaat caggtggaga
      181 tatttcatta aagtttacac agctaggagc agagacaaat gcctttacaa ctttcaacca
      241 aacgagtttc ttcttttcaa cagctagtaa atttcaggaa aatttggaac tccttcaata
      301 ttttgttctt tctgctaata taacagatga atctgttagt agagaaaaaa aaataatcgg
      361 gcaagaaatt gatatgtatc aagatgatgc tgactaccgt gcatatagtg gaatccttca
      421 gaacttattt ccaaaaacaa gtttagctaa tgatattgct ggctcaaaag catccattca
      481 aaagatcacg aaaatattat tggaaacgca tcatacttat ttttaccaac ctactaatat
      541 gagtctattc attgtcggtg atattgatat tgacgaaact tttttagcta ttcaaaggtt
      601 tcaaaccaca ttatcctatc cagatagaaa aagggtaact gttgatcctt tacactacta
      661 tcctgtcatt aaatcatctt ctgttgacat ggatgttact attgccaagc tggttgttgg
      721 ttttcgaggg tatctgacct taactcagca ttcactatta acgtatcgta ttgccttgaa
      781 gctattttta tcaatgctta taggttggac ttctaaaatc tatcatacgc tatatgaaga
      841 tggtaaaata gacgattcat ttgatgtgga tgttgaaatt catcatcatt ttcaatttgt
      901 attaatatca cttgatacac cagagcctat cgcaatgtct aactatatcc gtcaaaaatt
      961 agccactata aagatatcaa aagagtttac aaatgagcac ttaaatctat taaaaaaaga
     1021 aatgtatggt gactttattc agagtttaga ctctatagaa catttaaccc atcagttcag
     1081 tctatacctg tctgattctg ataaagaaac atattttgat attccaaaaa ttatagaaag
     1141 actcactcta aaagatgttg ttacaattgg aaaagcattt tttgaaaaag ctgatgcttc
     1201 agacttcaca gtcttcccaa aatagatgaa aatctgttat aataggatta ctatattatt
     1261 tttgaggtac ctgtatgagt gaaaaaagtc ttggtgaagt gttaagagaa tcgcgtgttg
     1321 gaaaaaatat cacactagat gatatagaat tgaagacagg gatttcttcc cattacttgc
     1381 tggcaatgga attggatcag tttaaaatta ttccggaaga aaaatttgat caatttttaa
     1441 aagaatatgc tgacattgtt gagttagatt tcaatacttt aaaaagacga taccgttatc
     1501 aagttaattc caaaaaagac agtgatataa aatctgtaac tgagattgtt gaagagaagt
     1561 taagtaagaa acgattacaa gagaagttgt tatcacatca gagcatgggg tatggtcaac
     1621 cgttagaagt ttctaaaaac aaagaaacct tagttatccc tgtaaagact gttggtttag
     1681 atagcaaatc gccactaaaa acgatatcgc ctgttagcca atttaaatca gataacaggt
     1741 taattcctaa aaaaaatgtg tcacgtatga ggcgaaatag cactgacagt cgttctaaaa
     1801 cgtcactatt tcccatagct atattaggaa cggttgcttt tgcaattatc agtgttatca
     1861 ttttaggtgt ttggcaacaa ttcgaaaaga gtcaaagagc taaagaagct gaagttgctt
     1921 tgcttaaagc ctctgagcag tctactaacg gtttaaaaaa taattcaaat gatcgcaaaa
     1981 ctcaagtcac aactgagggg tcgggtaatt atttaatagc gactgttact aaatctaaag
     2041 agaccgttga tatcagtgtg tctttaactg aggcacaaag tagttggatt tctctaacaa
     2101 attcagaaat tggtgaaggt ggtataacat tgactcaaga ctcgccaact tatacagcga
     2161 ctttacctgc agaaataaca gaatcattgt tgacacttgg tgtgacaaaa ggtgtatctg
     2221 taacaattga tggacaacct gttgatctat cacctttaac aagtactgac ttaagttata
     2281 tcacgtttaa gatccaataa ttaaggtaga ctaatgataa aaaaagaaaa tattcctaat
     2341 ctattaactc ttgttcgaat cgcaatgatt cctttctttc tttttattac ttcctcatct
     2401 aataaggtgg ggtggcatat ttttgcagct gtgatttttg caattgccag ttttaccgat
     2461 tatctagatg ggtatcttgc gcgtaagtgg catgtagcca gtaattttgg aaagtttgcc
     2521 gatcctctag cagataagat gcttgtcatg agtgccttta tcatgttagt tggacttggc
     2581 ttagttcctg cgtgggtgtc agctgttatt atttgccgag aattggcagt aactggtctt
     2641 cgattactac ttgttgaaac cggaggaaag gtccttgcag ctgctatgcc gggaaaaatc
     2701 aaaacagcga cacaaatgtt gtctattatt ttattacttt gccattggat attcctagga
     2761 aacgtcttac tctatattgc tctttttttc actatttatt ctggatatga ttattttaaa
     2821 ggagcaagct ttctttttaa ggatacgttt aaataacatg tcagctatta ttgaacttaa
     2881 aaaagttaca tttaattacc ataaagacca agaaaaacca acattagatg gcgtatcgtt
     2941 tcatgtgaaa caaggtgagt ggctgtctat cattggccat aacggatctg gtaaatcaac
     3001 gactattcga ttaattgatg gattattgga accagaatca gggtctatta ttgttgatgg
     3061 agatctacta accataacta atgtttggga aattcgtcat aaaattggta tggtctttca
     3121 aaaccctgat aatcagtttg ttggagcaac tgttgaagat gatgttgctt ttgggcttga
     3181 aaataaaggt attgcacatg aagatataaa agagagggtt aatcatgctt tagagttagt
     3241 cggcatgcag aactttaaag aaaaagaacc agcccgttta tctggtggcc aaaaacagcg
     3301 cgtagctatt gcaggtgcag ttgctatgaa gcctaaaatt attattttag atgaagctac
     3361 tagtatgctt gaccctaaag gacgattaga gttaatcaaa actataaaaa acatccgtga
     3421 cgactaccag ctgactgtta tttccattac tcatgactta gatgaagttg ctcttagtga
     3481 tagagtttta gtgatgaaag atggtcaagt ggaatcaaca tcaacaccag aacaattatt
     3541 tgcaagaggg gatgaactac tccaattagg tcttgatatc ccttttacaa catctgttgt
     3601 acagatgctt caagaagaag gttatcctgt tgactatgga tatctcacag aaaaggaatt
     3661 agaaaatcag ttatgtcaat taatctccaa aatgtaagtt atacttatca agcagggact
     3721 ccttttgaag ggcgtgccct ttttaacatt aatttagata ttttagatgg ttcttatacg
     3781 gcttttatcg gacatactgg atccgggaaa tcgaccatta tgcaattgct aaatggttta
     3841 cacgttccga caacgggaat tgtcagcgta gataaacaag atataacgaa tcactctaaa
     3901 aataaagaga ttaaatctat tcgaaaacat gtaggattag tttttcaatt tccagaaagt
     3961 caactatttg aagagacagt tttgaaagat gttgcttttg gccctcaaaa ttttggggtc
     4021 tctccggagg aagcagaagc tttagcgcgt gaaaaactag ctcttgttgg gatttcagaa
     4081 aatctttttg aaaagaatcc atttgaactc tcagggggtc aaatgcgtcg tgttgctatt
     4141 gcgggtatcc ttgctatgca gccaaaggtt ttagttttag atgaaccgac tgctggtctt
     4201 gatcctaaag ggcgtaaaga attaatgact atttttaaaa agttacacca atctggcatg
     4261 actattgttt tagtaaccca tctgatggat gatgtggcca attacgcaga ttttgtatat
     4321 gttcttgaca aaggtaaaat tattttatca ggaaaaccca aaacaatttt tcaacaagtc
     4381 agtttgcttg agaaaaaaca gttaggagtt cctaaggtga ctaaacttgc tcaaaggctt
     4441 gttgatagag gaatacctat ctcttctttg cctattacct tagaggagct aaaagaggta
     4501 ctaaaacatg gataagctga ttcttggtcg ttatattccc ggagattctc tcattcatcg
     4561 tcttgaccca agaagtaaat tattagcgat gattatttat attgtcatta tcttttgggc
     4621 taacaatgtt gtgacaaatt tgttgatgtt gacttttact ttagcagttg tttttttgtc
     4681 aaaaatcaag ttgtctttct ttttaaatgg ggttaaacca atgattggta ttatcctctt
     4741 cacgacttta tttcagatgt ttttctcaca aggaggaaaa gtaattttta gttggtggtt
     4801 cattagcata actgatttag gattgagcca agctatttta atttttatgc gtttcgttct
     4861 aattattttc ttttcaacgc tgttaacgtt gacgacaaca cctttaagcc tatctgatgc
     4921 agttgaatca ttattaaaac ctttaacaag atttaaagtt ccagctcacg agatcggact
     4981 aatgctttct ttaagtttac gttttgtgcc tactttaatg gatgatacca cgcgtattat
     5041 gaatgcacaa agggcccgtg gagttgattt tggagaggga aatttaattc aaaaagttaa
     5101 atctattatt cctattttaa tccctctttt cgcttcaagc tttaaaagag ccgacgcatt
     5161 agctattgct atggaagcta gaggttatca gggaggagaa gggcgaacta aatatcgtca
     5221 attagactgg cagctaaaag atagtttagc tattggaata gtttctttat tgggtttgct
     5281 actttttttc ttgaaaacct ctttgtaata ttttttagca caaaactttt ctttattagt
     5341 aagaagtgaa ttgtacctca aaagttagat acaacatcta atgattgagg tacttttctt
     5401 catttgagga attgaaaacc gctatctcaa ctatattaat cctcccaata ataacaaatc
     5461 gaacgaaatc taaaaggact gagtcctgtt caatacagaa ctaaatt
//
LOCUS       DATUYR010000079         5377 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.079, whole genome shotgun sequence.
ACCESSION   DATUYR010000079 DATUYR010000000
VERSION     DATUYR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.079"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..510)
                     /locus_tag="VCI33_001526"
     CDS             complement(<1..510)
                     /locus_tag="VCI33_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEP2832443.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILY"
     gene            complement(617..1312)
                     /locus_tag="VCI33_001527"
     CDS             complement(617..1312)
                     /locus_tag="VCI33_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEP2832444.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            1551..2486
                     /locus_tag="VCI33_001528"
     CDS             1551..2486
                     /locus_tag="VCI33_001528"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEP2832445.1"
                     /translation="MVSTATQIGHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            complement(2786..4648)
                     /gene="cadA"
                     /locus_tag="VCI33_001529"
     CDS             complement(2786..4648)
                     /gene="cadA"
                     /locus_tag="VCI33_001529"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEP2832446.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAETASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(5002..5277)
                     /locus_tag="VCI33_001530"
     CDS             complement(5002..5277)
                     /locus_tag="VCI33_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEP2832447.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
BASE COUNT         1657 a         1094 c          942 g         1684 t
ORIGIN      
        1 gtataagata cctgctaatt ctaaatcatg cttgatagct aaaaaggctt cttgactagg
       61 acacgaaatt aagtgactat gtagtccttc tgtcaacgaa gacaataact ctgctttaga
      121 ttgactgagt ttggacatga aattagttac atcagagtgt gtcttaatat ttaaaggggc
      181 tgttatcata ccgtaaatag gatgttctac ctctaccgtc gtaataatcc cttgatgagc
      241 taaaataatt tctaattctt ttttagtttc ttctacatta tgttggcaca caatacgtgc
      301 acaaaattgg tgactataaa gcgctgttga cataatataa ccttttggag tcgaaataat
      361 atcaatctgc tgagctcgaa gaagagctat atctccaacg attacttggc gactcactcc
      421 taataactta cctaaacgag tagcagatac agctttttgt tcactattta agcactcaat
      481 aattttttga cgacgatctt ctgctttcat aatctatcta tcctttttta tttctatcat
      541 atcatatttt taagcagggt tttctttctt cttaggatat agaaccaaaa aaccagacca
      601 aagtctggtt tttaagttat tcaccaccaa ggtagtattc tttaacaatg ttaagttttt
      661 catctaattc aaaaacaagt ggtgggaagt ttggaatttc cacatccatg atttcgtcat
      721 ctgaaagacc tttaatgtgt ttcacaagag cgcggattga gttaccatgt gcgccaacaa
      781 agacgttttt accatcaaga agagctggag caattttttc ttcccagtaa ggcattgcgc
      841 gttcaagagt tacttttaag ttttcagcat ctggaataag agctgggtca agatcagcat
      901 aacgacggtc tttgtgtgct gaatactcat catctttagc catagctggc ggcaatacgt
      961 cgtatgaacg acgccagata tgaacttgtt catcaccaaa ttgttctgca gcttcagctt
     1021 tgtttttgcc agtcaaagca ccatagtgac gctcgttcaa gcgccatgat ttttcagttg
     1081 gaacccacaa ttgacctgca ttttcaaggg caaggttagt tgttttgata gcacgtgtca
     1141 atactgaagt gaaagcaagg tcaaattcaa tacctgcttc tttgatcaat ttacctgcat
     1201 caatcgcttg ttgtgtccct ttttctgaaa gatcaacatc tgcccaacca gtgaagaggt
     1261 tagctttgtt ccattctgac tcaccgtggc gagcgaaaac caattttacc atgagttaat
     1321 atctcctttt aatttaaagg ttgctacctt tttacaattc ctattttaca aaaaatatag
     1381 caaaaaagct agtctattct gactttattg aaaatcataa tgtgactatc atgaaaattt
     1441 caaatgttcg tcaataacag taatatattt acatttttgt gaaaatagct tgctattatt
     1501 catgcctagt ggtagaatag aaaaaatttg tctaaaatat ggaggaaata atggtttcta
     1561 ctgctacgca aattggtcat ttctcttttg ataactgttt gatgaatgct gctggtgttt
     1621 actgcatgac aaaagaagag cttatggagg ttgaaaagtc tcaagccgct tcctttgtca
     1681 ccaaaacagg gacgcttgaa gttcgtccag gtaatccaga acctcgctac gcagatactc
     1741 gtcttggttc tatcaattca atgggactcc ctaataatgg ttttcgctac tacttagact
     1801 ttgtcagtga tcttgccaaa accgggcaac ataaacctca ctttctctcc gttgtcgggc
     1861 tatctcctac tgaaaccgaa accattttaa aagccatcat ggcttcagat tatgagggtc
     1921 tagtggaact aaacctttct tgccctaacg ttccaggaaa accacagatt gcttatgatt
     1981 ttgaaaccac tgatcaactt ctagagaaca tctttaccta ttacactaaa cctcttggta
     2041 tcaaattacc tccttatttt gacattgtgc attttgatca agcggctgcg attttcaata
     2101 aataccccct ttcctttgtt aactgtgtca attctatcgg aaatgggctg gtcattgagg
     2161 atgaacaagt cctcatcaag cctaaaaatg gctttggtgg tataggtgga gattacatca
     2221 aacctactgc tctagccaat gtccatgcgt tttacaaacg cttaaaacct tctatccaca
     2281 ttattggtac agggggagtt aaaacaggac gcgatgcctt tgaacatatt ctttgtggcg
     2341 ccagcatggt ccaaattggg acagcccttc atcaagaagg gccagctatc tttgaacggg
     2401 tcactaagga actaaaaacg attatggtag aaaaaggcta ccaaagctta gacgattttc
     2461 gtgggaactt acgttacaaa gattaacctt aatagtcaaa aaagcttgga aatgtgcact
     2521 ttgtacactt tccaagcttt ttaaaatggg aatttttttg aagggacagc gtcactttct
     2581 tgggactgtc tttgctggtt gtttgattca acttttcttt tttgcttgcg ctcaaattgc
     2641 tgataaaaat agaaaaatag aatgatttta attattgaaa atacagctaa accaatgatc
     2701 ataatagtaa acatacggcg gttttctctt tcataagtct tactattatt ataaacactt
     2761 tcaagttttc aagcaagttt ctaaattatt taaaagaaag taaacgcaag ccatttaaaa
     2821 tcactaaaat cgttgagcct tcgtgtccaa caacaccaag cggcaagtta actacctgaa
     2881 aaacattggc taaaatcaat aaggtaatca cagataaggc aaaaacaata ttttgtttga
     2941 taatggtctt cattttgcgg gaaagttgaa tcgaaaatgg aatacgagtc aagtcatcca
     3001 taatcacact gtctgcactt tccattgcaa tatctgttcc tgatccaata gcataagaaa
     3061 catctgcttg agcaagggca ggagcatcat taataccatc tcctaccatg gccacaaaac
     3121 catatttggt ctttaattct gctaacttag ccaccttatc ttgaggcata cagttggcta
     3181 ccacttcatc aataccaagt ttttgtgcca cataattggc ggttcgttct tggtcacctg
     3241 ttaacatgac tgttttgatt cccatggcat gaagagactt aatagcacgt tttgattcta
     3301 ttttgatatc atccaagagg gcatagtaag ctatcaattg atggtcacgt gaaacaaaga
     3361 ttagggtttt cccttgattt tcttccactt gaatagtttc ttcaaaagct gataggtctt
     3421 gaaccttttc caaaatgaag gttttcttgc caattcgcca ttcttgccct tgatagaagc
     3481 cctgaaaacc tttcccagaa atttcttcta agtggtcaaa ggtcagtggc tccaattttt
     3541 cagtgtattc aagaagggct ttagaaatag gatgggtact agcagtctct gctcctttta
     3601 ccagtctatt cacgagcaac tcatcttcca aataatgagc attcacaaca gaaggttttc
     3661 cttgggtgag cgttcccgtt ttatccatga caacagcctt aatatctccc atgttatcga
     3721 caatatcccc tcctttgata atcaatccct ttctggctgc acgagaaata gcagccaagc
     3781 tagcaggtgt ggaactggca attagggcac atggtgaagc tactgttaag agaatcatcc
     3841 ctcggtaaaa agcagccaac caagtccaag aaagcacaaa gtggctaaag aggataaagg
     3901 caggaataag gacgagcaca aatttgacat aaccatcttc taagctttcg ataaaggtgg
     3961 cagttttgct tttcttttct tgggcagatt ccaccagatt aataatcttg gcaaagagag
     4021 tatcgtcatt ttcaatagta accaacatat ctatggtttg tccttggtta atggttcccc
     4081 caatcagatc ctggccttct gccttatcaa cagtgatagg ctcaccagtg accatagatt
     4141 catcaaattg accaaaagga ctaagcaact gcccgtcaat tggaacagct tcgcctttac
     4201 gaacttgtaa acggtcacca acactcaagg atctggtctc aacttctaaa atatggccat
     4261 cttcttggta ttgacgagct gtatctggcg tcaaggacat caaggctgaa atagcatcct
     4321 tacttttttc catggccatt tcttcaagcg tattggacaa cgaaaagata aaaataagca
     4381 gagcaccctc cagccaatag ccgataattc cagcaccgat agctgctaaa atcatcaaaa
     4441 tatccactga caagtgtttg ttcttcacca aatccaatat acccgttttt gcagacgcat
     4501 atcctccaat caagaaggct gtaatgaaaa tagcggaagc cacttgtgga aatgagtgta
     4561 aaaaggctag gccaattatt attaatacca aacacgctaa agtctccatt aaatgaagat
     4621 ggtctgccat ccattgtctg attgtcattt ttagttccct cgcaattaaa agttattcaa
     4681 tctttaagat acattaatta taataattct aaataaataa gtcaagtaat ttagaagcat
     4741 tctaaataaa cttttttaaa cttacctttt gctttcaaaa atcctaactt cgactatttg
     4801 caaatatgaa aaaaactccc cctacctgcg gaaatgatat actaatagaa ggatgactga
     4861 ccattataga gtgcatgcat tagaacttgg cttaactgaa gagaaaacta ctaactttat
     4921 gccccaaata tgccccaaat ataaaaaaga accttgtcgc tatcaataaa catcgatagc
     4981 aacaagattt ttctagacga attatttaac agcgtcttta agagctttac cagctttgaa
     5041 ggctggaact tttgaagctg caatttcaat ttctgcacca gtttgtgggt tacgaccttt
     5101 acgagctgca cgttcgcgta cttcgaagtt accaaaaccg atcaattgta ctttttcacc
     5161 ttcagcaagg aaagcttcga ttgtagaaaa cacggcatca actgctgctg ctgaatcttt
     5221 tttagtcaat tcagttgctt ctgcaacctt tgcgattaaa tcttgtttgt tagccattta
     5281 acaaatcctc caaataatta gtaagcttaa tgctttaaca gatattatca tatctaaaaa
     5341 aagcgattag gtcaagtatt taacgttatt tgagctt
//
LOCUS       DATUYR010000080         5200 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.080, whole genome shotgun sequence.
ACCESSION   DATUYR010000080 DATUYR010000000
VERSION     DATUYR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.080"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            173..1342
                     /locus_tag="VCI33_001531"
     CDS             173..1342
                     /locus_tag="VCI33_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP2832448.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGQVLTSTEVVSVKKQDDLFYLKSTDQTFTCQKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            complement(1437..2108)
                     /gene="deoC"
                     /locus_tag="VCI33_001532"
     CDS             complement(1437..2108)
                     /gene="deoC"
                     /locus_tag="VCI33_001532"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEP2832449.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIQNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAKTFIALGASRLGTSRIIKIVKNEAT
                     KTDSY"
     gene            complement(2138..3340)
                     /locus_tag="VCI33_001533"
     CDS             complement(2138..3340)
                     /locus_tag="VCI33_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEP2832450.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            complement(3361..4140)
                     /gene="udp"
                     /locus_tag="VCI33_001534"
     CDS             complement(3361..4140)
                     /gene="udp"
                     /locus_tag="VCI33_001534"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEP2832451.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            4380..5138
                     /locus_tag="VCI33_001535"
     CDS             4380..5138
                     /locus_tag="VCI33_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP2832452.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
BASE COUNT         1687 a         1112 c          858 g         1543 t
ORIGIN      
        1 aattaaataa aaaagcaacc gttttcataa aaagataatt ttaatcatct ggactatttt
       61 tctagctttt tgatgtcatg gaggcattgt ttagatacgt aaaaaaacgg tatgccaggt
      121 tagttcattt acctgactat taaacccccc gcttttatgc taaaataagg ctatgactca
      181 atacgatacg attattattg gaggtggccc tgctggtatg atggccgcca tctccagcag
      241 ctactatggc tataaaactc tcttaattga aaaaaatcgc cgcttaggta aaaaattagc
      301 gggaaccggt ggtggacgct gtaatgtcac taatagtggt aaccttgatg accttatggc
      361 aggcattcct gggaatggcc gctttttata tagtgttttt tcccagtttg acaaccatga
      421 catcattgcc tttttcgagg aaaacggcgt caaactcaaa gaagaagacc atggacggat
      481 gttccccaca acagataaat caaggacaat cattgatgct ctggagaaaa aaatcaaggc
      541 tcttgggggt caggtgctaa ccagcactga agtggtttcc gttaaaaaac aagatgacct
      601 cttctacctc aaatctacag accagacttt cacctgccaa aaattgattg tgaccacagg
      661 aggcaagtcc tacccttcaa cgggttcaac aggttttgga catgatattg cacggcattt
      721 taaactgacc gtgactgatt tagaagctgc tgagagccct ctgttaactg attttcctca
      781 caaagtcttg caaggtattt ccctagacga cgttactctt agttatgaca aacacgtcat
      841 cacacacgac ttactcttca cccactttgg cctttcagga cctgcagctc ttcgtctgtc
      901 ctcttttgtg aaaggtggag aaattgcaga gctggacttt ttgccacact tatcaacaga
      961 tgacttaacc gcttatctga gtgatcaacg tgataaaaac atcaaaaatg ctcttaaagg
     1021 cctactgcct gaacgagtag ctgatttttt atcagaagat taccccgaaa aggtcaaaca
     1081 actctctcca aaacaagaaa aggaattgct tgataaactc aaacacctcc agatacccat
     1141 tacaggaaaa atgtctttgg ctaaatcatt tgtgaccaaa ggaggcgtag atttaaaaga
     1201 aatcaaccct aaaaccttgg aaagcaagaa agttcctggc ctgtatttcg ctggagaggt
     1261 cttagatatt aatgctcaca ccggagggtt taatattacc tcagctcttt gttcaggatg
     1321 gatcgcaggg aagtcttcat agctaaaaaa gcctattatc actaagatag taggcttttt
     1381 aactcctatt atatgagttt tttgttttag tctcaagcta aatcggtatg attaagttaa
     1441 tagctatcgg tttttgtagc ttcgttctta acaatcttga tgatacggct agtacccaag
     1501 cgtgaagctc ctaaagcaat aaatgtttta gcatcttcca atgatgagat tccacctgct
     1561 gccttaattt taacaccttc gccgacatat tttgccatca cttcaacatc ttcaaatgta
     1621 gcacctgctg tcgaaaaacc agtagaggtt ttaataaagt ctgcacctga acgtgtgaca
     1681 actccacaaa gttcgataag ttcttcttta gttaattgac atgtctcaac gataactttt
     1741 aagatatggt cttgacatgc ggctttgatt tgacgaattt cttcttcaac agtatcaaaa
     1801 tccccatttt taacgtctgt caaattaatg accatgtcaa tttcatcagc accattttga
     1861 atagcatctt gacattcaaa aaccttcgct gcagttgtac tatagccatt tgggaaccca
     1921 ataacagtac aaatagctaa tttacctgaa acgtattctg ctgctttttt gacgtaagaa
     1981 gctggaatac atgctgaagc tgtttcataa gccatggcat catctaaaat tgtttggatt
     2041 tctggccacg ttgctgttgt tgctagcaaa gtatggtcta ccgtttttaa aatatctttt
     2101 acttccacgt tactatcctt ctttcttttg tcaacgttta gaatagagtt acaataccga
     2161 cgataaaggc gctaagcata cttacagcaa taccaccaat cattgcacgg aaaacaagtc
     2221 gagctagggt gctacgtttc tccgggcaaa gaacagcaat acctgaaaca caaataccta
     2281 aacttgataa attagcaaaa ccacagagtg aaatagttgc taccaatgct gtacgataat
     2341 ctaaagattt gattaggtga cccaattgtt ggaacgaaac aaactcattt aaaatcaact
     2401 tacttccaag aaggtttcct tctagaagaa tgtttttgtg gtcaaatccc ataagaaaac
     2461 caaatggagc aaaaacatat gagaagattt gttctaagcg aattcccaat ccacttaaca
     2521 tcatattaat caaagaaact aaaccaacaa aggcaatcaa actagcccca attgagaaag
     2581 ccatttgtgc acctgtgctt gcaccctcag cgattgcatc aatcacattg gcgttattac
     2641 ctttattatc catcttaatg tcatcaattt tttgaacagg ttctgtttga ggcaataaga
     2701 ttttagcaat gagaatactg ccaataggaa ccattgttga agcaatcaag agatattcca
     2761 ttggaatgcc taatgcaata tagccaccaa gaatagaaac tgacatactt cccatacctg
     2821 ataccaacac aaccattatc tcactatcag tcatacgacc taggtatttg ctaaccaaaa
     2881 ttggactgtc tgtttgacca agaaacatat tagctacggc aacaaaactc tcaacctctg
     2941 aggatttcat aattttacca acgcccttac ctatccattt tactacaaat ccaaggattc
     3001 ctacataata aagtagacta actagggcag ataagaaaac aatattacca agcgtttgaa
     3061 tagcgaaaat aaaaccagtt tttgcgccac tatctgctaa tgacccaaac acaaaattta
     3121 aaccagcttg accacagttg attacgctag taactccagt tgaaacaaca ctaacaattt
     3181 gttggcctag tgggatacgt actaagatta gcgcaataat gaattgaacg ataagagctt
     3241 ttccaattaa acttagagaa acactcttac gattgaaaga aattgcatac acaattccta
     3301 ataccaataa aataccaata atactataaa taaattgcat gatgacttcc aaaacctcgc
     3361 ttattgtgat ttatcatttt caataagtgt gcggagtgct tcaacagcaa cttgaatagc
     3421 tgcttctgta tcatgagcca ttgggttatc catacccaaa gcgttgcgtt cttgattacc
     3481 aacaacaagg aaatctgatc cacaacgaac tcctagatga cttgctgcta caaaaagagc
     3541 tgcagattcc atttcagacg cttttgttcc cagacgtttc catgcttccc atttatttaa
     3601 taattcataa ctaactggca tacgttctgg ctcatgttgc ccgtaaaatg catctttaca
     3661 ttgaactact cctgcgtgac ttgtataacc aagcttttta gctgcattaa ctagagcatt
     3721 tgtcacttcc aagtcagcta cagcaggaaa ttcaatcgga gcatattctt tactggtccc
     3781 ttccatacgg atagctccag ttgcaataac gatgtctccg cctttaacat caaggtcaat
     3841 accaccacat gttccaacac ggataaaggt atcagcgcca cagagtttca attcttccat
     3901 cgcaatagac gctgatggac caccaatacc agttgaagta acactaactt tttcaccgtt
     3961 taatgtacct gtatacgtaa catattcacg gctgtctgct acaagaactg cattatcaaa
     4021 atgttctgca atttttgcac aacgttttgg atcacctggc atgataacat aacgacctac
     4081 atcacctgga cgaatctgta ggtgatattg caatccgact tcacctgaat aattttgcat
     4141 gttaaaaacc tcctgtattt tacttgtcta atttttgata cacttgtata gtatcacaca
     4201 cactctattt tgtaaacgct tttttaaggt gtttttcatc tcttttttga caaaaagtac
     4261 ctgagtgtga gatactaaca ctatagatgg gtacaattct aatcaaggaa tactgttagt
     4321 gctaaaatag tttttagaca agttaaactc cactaaagat tattaatgag gtaagtgata
     4381 tgtctactaa cgacttaacc aaaaaactca aaaaacttaa acatgtgcaa gtatataata
     4441 ctatttttca acttattcaa gatggtacat atagtcctgg catgcaactg ccatcagaac
     4501 ctgaacttgc tagacagctc aatgtcagtc ggatgacact gcgtaaatcc ctagccctcc
     4561 tccaagagga tcatctcata aaaaatatca gaggaaaggg aaattttata ctcaagactc
     4621 ctgaaactaa atatcaccaa ggtttcgaat atcttcaaca ccccatatat gcaagcctat
     4681 catctgacat cacaaaggtt gaattggaat atcggattga agtgcccact gttgccatta
     4741 cagcatccct aaaacaagaa actcctgttg tgattattgt tgatcgctgg tatcatagcc
     4801 aaaataaagc tattgcttat agtttatctt ttatccctat tgaagttatt tctaaatatg
     4861 ctataaatct caatcaagaa gagccccttc ttactttctt agaagagaaa atctatgaat
     4921 ctggtaaagc ttctcattcc tgcaatcaaa tcggctatac caagactggc aattacacag
     4981 caactaagta tactctatca gaaaatagtg cttttatttt aatccaagaa actctctaca
     5041 atggtaaaga catcttggtc tcaaccaaac actacgttcc tgctgattta tttgacttaa
     5101 aagttcaatc tcaaagttgc caaagcgaca ctcattaaac acctttaaac agttattaat
     5161 acctgactcc cacacttaga tgtggagttt tttgatatta
//
LOCUS       DATUYR010000081         5156 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.081, whole genome shotgun sequence.
ACCESSION   DATUYR010000081 DATUYR010000000
VERSION     DATUYR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.081"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            101..1649
                     /locus_tag="VCI33_001536"
     rRNA            101..1649
                     /locus_tag="VCI33_001536"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1701..1773
                     /locus_tag="VCI33_001537"
     tRNA            1701..1773
                     /locus_tag="VCI33_001537"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1734..1736,aa:Ala,seq:tgc)
     gene            2074..4976
                     /locus_tag="VCI33_001538"
     rRNA            2074..4976
                     /locus_tag="VCI33_001538"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5062..>5156
                     /gene="rrf"
                     /locus_tag="VCI33_001539"
     rRNA            5062..>5156
                     /gene="rrf"
                     /locus_tag="VCI33_001539"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1461 a         1063 c         1481 g         1151 t
ORIGIN      
        1 agacgaacca aacgtgaggg tgatatggag acatattacc cgtcaagaaa cgagaaataa
       61 atctgtcagc gacagaagga acgagtaagt tcaaacgcaa attaaagaga gtttgatcct
      121 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct gagaactggt
      181 gcttgcaccg gttcaaggag ttgcgaacgg gtgagtaacg cgtaggtaac ctacctcata
      241 gcgggggata actattggaa acgatagcta ataccgcata agagagacta acgcatgtta
      301 gtaatttaaa aggggcaatt gctccactat gagatggacc tgcgttgtat tagctagttg
      361 gtgaggtaaa ggctcaccaa ggcgacgata catagccgac ctgagagggt gatcggccac
      421 actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa tcttcggcaa
      481 tgggggcaac cctgaccgag caacgccgcg tgagtgaaga aggttttcgg atcgtaaagc
      541 tctgttgtta gagaagaatg atggtgggag tggaaaatcc accaagtgac ggtaactaac
      601 cagaaaggga cggctaacta cgtgccagca gccgcggtaa tacgtaggtc ccgagcgttg
      661 tccggattta ttgggcgtaa agcgagcgca ggcggttttt taagtctgaa gttaaaggca
      721 ttggctcaac caatgtacgc tttggaaact ggagaacttg agtgcagaag gggagagtgg
      781 aattccatgt gtagcggtga aatgcgtaga tatatggagg aacaccggtg gcgaaagcgg
      841 ctctctggtc tgtaactgac gctgaggctc gaaagcgtgg ggagcaaaca ggattagata
      901 ccctggtagt ccacgccgta aacgatgagt gctaggtgtt aggccctttc cggggcttag
      961 tgccggagct aacgcattaa gcactccgcc tggggagtac gaccgcaagg ttgaaactca
     1021 aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg
     1081 aagaacctta ccaggtcttg acatcccgat gcccgctcta gagatagagt tttacttcgg
     1141 tacatcggtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa
     1201 gtcccgcaac gagcgcaacc cctattgtta gttgccatca ttaagttggg cactctagcg
     1261 agactgccgg taataaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat
     1321 gacctgggct acacacgtgc tacaatggtt ggtacaacga gtcgcaagcc ggtgacggca
     1381 agctaatctc ttaaagccaa tctcagttcg gattgtaggc tgcaactcgc ctacatgaag
     1441 tcggaatcgc tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta
     1501 cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg taacctatta
     1561 ggagccagcc gcctaaggtg ggatagatga ttggggtgaa gtcgtaacaa ggtagccgta
     1621 tcggaaggtg cggctggatc acctcctttc taaggaaatg gaacacgttt atcgtcttat
     1681 ttagttttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc
     1741 aggaggtcag cggttcgatc ccgctaggct ccatcaggat acaatcctac taaacttaat
     1801 acaagtgaag ttgaacacgc aactcacttc ctaggaaaat agacaatctt cccttgtgtg
     1861 caaggcccac atggtcagat tcctaatttt ctacagaagt ttcgctaaag cgagcgttgc
     1921 ttggtatcct atataatagt ccattgaaaa ttgaatatct atatcaaatt ccacgatcta
     1981 gaaatagatt gtagaaaagt aacaagaaaa taaaccgaaa acgctgtgac tatttgataa
     2041 gttttctagt ttttaaaaaa actaggttaa taaggttaag ttaataaggg cgcacggtgg
     2101 atgccttggc actagaagcc gaagaaggac gtgacaaacg acgaaatgct ttggggagct
     2161 gtaagtaagc gctgatccag agatgtccga atgggggaac ccactaacta atggttagta
     2221 tccataactg ttaaggttat gagaaggaag acgcagtgaa ctgaaacatc taagtagctg
     2281 caggaagaga aagcaaacgc gattgcctta gtagcggcga gcgaaacggc aggagggcaa
     2341 accgaagtgt ttacacttcg gggttgtagg actgcgacgt gggactttaa aaggatagaa
     2401 gaattacctg ggaaggtaag ccaaagagag taaaagcctc gtatttaaaa ttcttttgag
     2461 ccctagcagt atcctgagta cggcgagaca cgtgaaatct cgttggaatc tgggaggacc
     2521 atctcccaac cctaaatact ctctagtgac cgatagtgaa ccagtaccgt gagggaaagg
     2581 tgaaaagcac cccgggaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtt
     2641 cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag ttacgatatg
     2701 atgcgaggtt aagttgaaga gacggagccg tagggaaacc gagtcttaat agggcgattt
     2761 agtatcatgt tgtagacccg aaaccatgtg acctacccat gagcaggttg aaggtgaggt
     2821 aaaactcact ggaggaccga accagggcac gttgaaaagt gcttggatga cttgtgggta
     2881 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
     2941 agcgtcgatg ttaagtctct tggaggtaga gcactgtttg ggtgaggggt ccatctcgga
     3001 ttaccaatct cagataaact ccgaatgcca acgagatatc atcggcagtc agactgcgag
     3061 tgctaagatc cgtagtcgaa agggaaacag cccagaccac cagctaaggt cccaaaataa
     3121 ctgttaagtg gaaaaggatg tggggttgca cagacaacta ggatgttagc ttagaagcag
     3181 ctattcattc aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     3241 ccggggctaa aacagtttac cgaagctgtg gatgacacaa aagtgtcatg gtaggagagc
     3301 gttctatgtg tgaagaaggt gtaccgtgag gagcgctgga acgcatagaa gtgagaatgc
     3361 cggtatgagt agcgaaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg
     3421 ggaaggctcg tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga
     3481 tggccaacag gttgatattc ctgtactaga gtatatagtg atggagggac gcagtaggct
     3541 aactaaaccg gacgattgga agagtccggc taagcagtga ggtgtaagat gagtcaaatg
     3601 cttatcttta taacattgag ctgtgatggg gagcgaattt tagtagcgaa gttagtgatg
     3661 tcacactgcc aagaaaagct tctagcgttt aatgatactc tacccgtacc gcaaaccgac
     3721 acaggtagtc gaggcgagta gcctcaggtg atcgagagaa ctctcgttaa ggaactcggc
     3781 aaaatgaccc cgtaacttcg ggagaagggg tgctgactta ggtcagccgc agtgaatagg
     3841 cccaagcaac tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg
     3901 ggtgacgcct gcccggtgct ggaaggttaa gaggagggtt tagcgcaagc gaagatctga
     3961 attgaagccc cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt
     4021 gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag
     4081 actcggtgaa attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc
     4141 ccatggagct ttactgcagt ttgatattga gtatctgtac cacatgtaca ggataggtag
     4201 gagccattga cttcgggacg ccagtttcga atgaggcgct gttgggatac tacccttgtg
     4261 ttatggctac tctaacccag ataggttatc cctatcggag acagtgtctg acgggcagtt
     4321 tgactggggc ggtcgcctcc taaagagtaa cggaggcgcc caaaggttcc ctcagattgg
     4381 ttggaaatca atcgcagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc
     4441 gagcagggac gaaagtcggg cttagtgatc cggtggtacc gaatggaagg gccatcgctc
     4501 aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg
     4561 gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg
     4621 gttgggctgt tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt
     4681 tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg
     4741 accagagtgg acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct
     4801 atgtagggaa gggataagcg ctgaaagcat ctaagtgcga agcccccctc aagatgagat
     4861 ttcccatgat tttatatcag taagagccct gagagatgat caggtagata ggttaggagt
     4921 gtaagtgtag cgatacatgt agcggactaa tactaatagc tcgaggactt atccaaaaag
     4981 aaatattgac aacgttacgg attcttgtta gactatagat attcaatttt gagtgggtta
     5041 tgaaaatagc acatttacaa gttaagtgac gatagcctag gagatacacc tgtacccatg
     5101 ccgaacacag cagttaagcc ctagaacgcc tgatgtagtt gggggttgcc ccctgt
//
LOCUS       DATUYR010000082         5020 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.082, whole genome shotgun sequence.
ACCESSION   DATUYR010000082 DATUYR010000000
VERSION     DATUYR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.082"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            33..902
                     /locus_tag="VCI33_001540"
     CDS             33..902
                     /locus_tag="VCI33_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEP2832453.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDGINYTQKIGVMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEAFKPKQEGITTYFGGSVTYTSNGSHEGR
                     EAYERVATGTSYNRNNDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            856..1206
                     /locus_tag="VCI33_001541"
     CDS             856..1206
                     /locus_tag="VCI33_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEP2832454.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGHMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKKFMEVMASRGALVGVAR
                     SVEDALNIVNDTSR"
     gene            1190..1546
                     /locus_tag="VCI33_001542"
     CDS             1190..1546
                     /locus_tag="VCI33_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832455.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNITIADKEEADRVYRSVKYYKKL
                     NRLPPPDVLEWFQRIYTTKEMIMLIKQSYRLKQKKTDEDDKIYEKWMFKNYGDVKLVK
                     KIKRTDALERARRMGL"
     gene            1788..2024
                     /locus_tag="VCI33_001543"
     CDS             1788..2024
                     /locus_tag="VCI33_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEP2832456.1"
                     /translation="MIEELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLIEYWLIESVEE"
     gene            2021..2191
                     /locus_tag="VCI33_001544"
     CDS             2021..2191
                     /locus_tag="VCI33_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832457.1"
                     /translation="MKRYIEVKDEWKSATDHLNDFIDKNKYAKVTVVGYQVVQLSPYG
                     RDLTYILAEVEE"
     gene            2188..2472
                     /locus_tag="VCI33_001545"
     CDS             2188..2472
                     /locus_tag="VCI33_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832458.1"
                     /translation="MTIDEALQNLRDNFNKIMNVLKNDWKALLFLAIAIFGMMVTVSY
                     FSYRDARQYYESQITGLRTQLSRTQKQLKRASEDRARQTKRIAEFTHNGG"
     gene            2474..3106
                     /locus_tag="VCI33_001546"
     CDS             2474..3106
                     /locus_tag="VCI33_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEP2832459.1"
                     /translation="MIKIDEIHRILGIDEVYKAPKRLTDILFDKDSREDIFRQFLKYE
                     TDVSYDWFMQYFEEEQADRKNKKQDFTPKSVSTLLSKIISGNQYYEVAVGTGGILIQA
                     WQEQRLNDSPFTYRPSKYWYHVEELSDKAVPFLLFNMSIRGINGVVVHGDSLTRQVKN
                     IYFLQNTKDDMLSFSDINVMPRTQDIEQEFNVKEWIGDAIVHVESKLNAR"
     gene            3111..3590
                     /locus_tag="VCI33_001547"
     CDS             3111..3590
                     /locus_tag="VCI33_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEP2832460.1"
                     /translation="MNIEEAIKKIRELEAFNYFDFKGNLIRRIDVLGIIDQLKIIVQQ
                     CVADWYEKHKDNLEYDLYLYQMSIYDEKVEEDDFYYWMQKSKNPVRTLINMHQFGYEI
                     EQEQLYTVDLPNGQPLVRGKNTLYFSQNLTAENAKLTESEIRKDFEWAWQFAKEVTE"
     gene            4039..4473
                     /locus_tag="VCI33_001548"
     CDS             4039..4473
                     /locus_tag="VCI33_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEP2832461.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPHGVPSKPVERLALNRVSAEQELDAIEQAVSMILEPERRRILYDKYLAPYKKAD
                     KVIYTELCMSESFYYDTLDIALLAFAELYREGVLLVEQGVFS"
BASE COUNT         1791 a          790 c         1075 g         1364 t
ORIGIN      
        1 gttacgtagt aaaaaagggg gtgcgttacc ttatgtacca cacaacagca ctttcattct
       61 taaaaaaagg atatcaggtt ataccgctta gaaaagatac tggaacgcca atgattaagt
      121 ttaaagatat ccaaataacg gaagaagtga tcaaaaatac aaactggttt aattgtgatt
      181 atgctttatt aatgcgtggc atttggtgta ttgatattga tactcatgac atggacgaga
      241 agttagctaa agagttatac ataatgataa aaaagatggg gattgattta ttatctgtat
      301 tatcgactga taagtatgac aatggactag atggttattc gtcaattatt aggcatgagt
      361 ataaaaacga attaatcagt aattttaaaa atacattcgc agaattaact gcaagtggtg
      421 gtatgcacat actctttaaa aaacgcgatg gtattaatta cacgcaaaaa ataggagtaa
      481 tgcctggtgt tgacataaaa gcgaatgata ataattttgt caaaatattc ccatctgacg
      541 gacgtgaagt tttacaagca gttaaaacat taccttatta tgatggaaaa tttgaagagg
      601 aagcatttaa accaaaacaa gaaggtataa caacctattt tggaggttct gtcacatata
      661 catcaaacgg aagtcacgaa ggccgagagg cttatgaacg tgtagcaact ggaacatcat
      721 ataacaggaa taatgatttg tttaaaggag cgtgttgggc gtttgaaaat ggtatcgata
      781 ttgatgattt aacatcaatt attgggacgg ttaaaggcag agacgtattt acgagagagg
      841 agtttgaatt aacgattgaa tcagcgaaac gaaaagtcag ctacgtcact atcagaacat
      901 gatatccaaa accttatccg aatggaatta tcacaagcgg ggcatatggt atttcgagca
      961 aatgtcggaa aggtgaaaac agcagatggt agattttttg atacaggttt accaaaaggt
     1021 ttttgtgatt tgtttggatt taaaccaaac ggacaaatat tttttatcga agttaaaaat
     1081 gaaacaggtc ggataagacc agaacagaaa aaatttatgg aggtcatggc atctagagga
     1141 gcgcttgtag gagtggctcg gtcagtggaa gatgccttaa acatagtcaa tgacactagt
     1201 agatgatttt tacaaacaaa tggagccgtc aattaaagcg tttttagacg ataacattac
     1261 catcgcagac aaagaagaag ctgacagagt ctatagatct gtcaaatact ataaaaaact
     1321 aaacagattg ccgccacctg atgtattgga gtggttccaa cgaatttaca cgacaaagga
     1381 aatgataatg ttaatcaagc agtcttaccg ccttaaacaa aaaaagacag atgaggatga
     1441 caagatttac gaaaagtgga tgtttaaaaa ctacggtgac gttaagctcg ttaaaaaaat
     1501 caaacgcacg gacgcactag aaagagctcg gagaatgggt ctatgaaaag acacagacag
     1561 tggcataacg atattaaata tacacctaga tcttacgata atctgttgcc ttacgatata
     1621 tcagagctgt taatagctca cagatgcaaa ataaagatgt ctgatgacgt tttagcagat
     1681 aagataggca tttatacttg gcaattaaaa gcgctcttag aacgcagaat attgccaaat
     1741 gagagcgagt gtaaattgat tataaatttt ttgagagagg tggagtgatg atagaagaat
     1801 tagtacataa accaaagcat tataatcacg gagaaattga tttgattgag tcgtggtata
     1861 agacgtaccc atgggaccag tttgtcgcaa tcatggaaag tcacattgat aaatacatta
     1921 aacgacatag atacaaaggc aatgcgactc aggatttaga aaaagcactc gaatatacaa
     1981 agcgtctcat tgaatattgg ttaatcgaaa gcgtggaaga atgaagagat atattgaagt
     2041 taaggacgaa tggaaaagtg caacagacca cctgaacgat tttatcgaca aaaacaagta
     2101 cgcaaaagtg acagttgttg gttatcaagt cgtacaactt tctccttacg gaagagattt
     2161 gacttatatt ttggcagagg tggaagaatg acaatagatg aagcgttgca aaatttacgt
     2221 gataacttta ataaaataat gaatgtccta aaaaacgatt ggaaagcact attgtttctt
     2281 gcaatcgcaa tatttgggat gatggtaacc gtgtcgtatt ttagctatcg cgacgcacga
     2341 caatattacg agtcgcaaat cacaggacta cgtacacagc taagcaggac acaaaagcag
     2401 cttaaacgtg ctagcgaaga tagagctaga cagacaaagc gaattgcgga atttacgcac
     2461 aacggagggt aacatgatta agatcgatga gatacatcgc atactaggca tcgacgaagt
     2521 ttataaagca cccaaacgac ttacggacat actctttgat aaagatagtc gtgaggatat
     2581 atttagacag tttttgaaat atgaaacaga tgtatcttac gactggttta tgcaatattt
     2641 tgaggaagaa caagctgacc gcaaaaataa aaagcaagac tttacgccta aatcggttag
     2701 tacactatta tctaaaataa taagtggtaa tcaatactac gaggtagcag tcgggacggg
     2761 tgggatactt atccaagcat ggcaagaaca acgattaaat gacagtccat ttacttatcg
     2821 tccgagtaaa tactggtatc acgtagaaga gttatcggac aaagcagtac cgtttctact
     2881 ctttaatatg tctataagag ggataaatgg tgtggtggtg catggtgact ctttaacaag
     2941 acaagttaaa aacatttatt ttttgcaaaa tacaaaagac gacatgctga gttttagtga
     3001 tattaacgtt atgccaagga ctcaagatat tgagcaagaa tttaatgtca aagagtggat
     3061 tggtgatgcg atagtgcatg tagagagtaa gttaaatgcg aggtaacaaa atgaacattg
     3121 aagaagctat taaaaagata agagaactag aggcgtttaa ttatttcgac tttaaaggta
     3181 atttaattag aagaattgat gtattaggta tcatcgacca actaaaaatt atagtacaac
     3241 aatgtgttgc agattggtac gaaaagcata aagataattt ggaatatgat ttatatttat
     3301 atcaaatgtc aatctatgat gaaaaagttg aagaagatga tttttattat tggatgcaaa
     3361 aatcaaagaa tccagttcgt actcttatta atatgcacca atttggctat gaaatcgagc
     3421 aagagcagct gtatacagtt gatttgccaa acggtcagcc tttagtacgc ggtaaaaaca
     3481 ctttgtattt tagtcaaaat ctaacagccg aaaatgcaaa actcactgaa tcagaaatcc
     3541 gaaaagactt cgagtgggct tggcaatttg cgaaagaggt gactgaatga ctgaagagtt
     3601 aggagtgtta tatagcgaaa aatggcataa gtattattta tataaaactt gcaggtatat
     3661 atcttatgtt gataatccac atcaggctac taaatgcacc cgcaaacaag cagaacagtt
     3721 tccacagttt aaatgggtat cgctgacaaa attataaccc cacgcaagcg ctcaagagcc
     3781 tgcgatggct ctgtgggggt ggaccgaaat taaaaaatag aaacgagaac ctccttacac
     3841 caaaacaaat ctaaagcggg ttatcggtca tccgtgatta tccaaggcgt cgctaatgct
     3901 ttaacacgac atcgtgcgcc tgtgtcaaaa aacaaaggaa agagagggct tttctccaca
     3961 aaacaaaaag acgtccacac ggaacgcccc cttggttaaa tttaagctta aataaattat
     4021 accacatcgg gggctttcat gacgtttttt ccagaaatta atatacaaaa aactaaatca
     4081 aacgctaagc gaaaactaag agagtatcca cgctggcgaa ggatagctaa tgacgtagat
     4141 actcaaaaag ttacagctac ttactcattt gagccaaggc aaccgcatgg ggtccctagt
     4201 aaaccagttg agagactagc gctaaaccgt gtgtcagcag aacaagagct ggatgcgatt
     4261 gagcaagccg tcagtatgat actagagcca gagagacgca ggattttgta tgacaaatat
     4321 ttagcgcctt ataaaaaggc agataaggtt atttatacag aattgtgtat gtcagagagc
     4381 ttttactatg atacacttga cattgctttg ttagcttttg cagagctgta cagagagggt
     4441 gtgttgcttg ttgagcaagg agtttttagc tagtttttat acagtaataa gatagtttat
     4501 acatattttt acatgttatt atagtactat caaaataaca agaagagata accttttaat
     4561 cactgactat tttatttagt cgccaacttt aactacgatc aaacttgtta ttttatggta
     4621 tgtgggacgt gcaggttcga atcctgccgt ctctatcagc gtcaggggag acgcgacccc
     4681 acacccattt aaaggtagac acgatctgat gtgtggggtt ttatgattaa ccgcaagtaa
     4741 aacaagggtc gcaaccttgc ttgtggttag taggcttaat ggtcgtttat gcacgttcga
     4801 ttcgtgcaaa gcctgttagg aacatgaacc gtgattggaa aacggtggag gtagcgcctt
     4861 gcttcgggac gttagctaag tccgaaaact ctttgcgaag agccagaaga agatgtgtcg
     4921 gttcgattcc gactgttcct gttgcattta atgcaaactc catattttta ttaaaaaagc
     4981 ctattatacc tattgtgtaa gggctttttt cgttgtatta
//
LOCUS       DATUYR010000083         5004 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.083, whole genome shotgun sequence.
ACCESSION   DATUYR010000083 DATUYR010000000
VERSION     DATUYR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.083"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..872)
                     /locus_tag="VCI33_001549"
     CDS             complement(<1..872)
                     /locus_tag="VCI33_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832462.1"
                     /translation="MDKIDDGLIASTFQKVKDVDIFALKAYMESTEGLETGLQVTLTD
                     IFVNFPFFLLNLIVGFFSIILRFFENFSLYNTYRQTVFDSSKVLWDNLSKSADFTNSL
                     LYLLIALSAFSIFLAFIFSKGDFSRRLLHLFAVMLLAFGYFGTVQSTSGGVYVLDMVH
                     SVASSFSDAVTNLSVVSPEDEKQIISHEASVADNYVMKTSYTAYLFVNTGQLNGKFHN
                     NKSGKEEELDNSKILGKYDKSNQFVIPKSQQILEYTNKLGDGALKGEEQNRWMSAVSD
                     YIWIKSIYVILKIF"
     gene            complement(887..3388)
                     /locus_tag="VCI33_001550"
     CDS             complement(887..3388)
                     /locus_tag="VCI33_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP2832463.1"
                     /translation="MVKKLEYPINSIHSNLALRKDKVVIAYYRIPNTPITITDSDKKD
                     SHKDKVAQVIKKLAKYKHFDISLVPKDYLLEEKMSDFIGDLADDVKDLGIDTLSYTVD
                     TLTAEIEIPYQFDWVIGVNLGRDTMSATLKELAVKQLDLIADNVASLLGYTVELDKEW
                     YKEYQDEELLLYGLLSSLKAKRLSEEDLFYYQRMQYLRYIPHAKNEVIANRTLLNVTD
                     TLVKPLSGGFLKLESPYGSSFITILPVGKFNTIFNGFHLGELVQRMSFPVELRFKAEF
                     INKTKLSGTMGRSNTRYDQIMKEAYSTNTVQQDDILMGSYSLKDLMKKVGNKEEIIEY
                     GCFLVVSGSSIKQLRQRRHAVLSYFADMQVNVHEASHDTPYLFQTLLYGQNLQKTTRK
                     WNHLVTSRGFSEMMLFTNTQSGNRIGWYIGRVDNRLSAWDNIGEAIMGSKNLVLFNAT
                     VANKEDVAGKVTKNPHIIITGATGQGKSYLAQMIFLHTAQQNVRVLYIDPKRELRKHY
                     LQVVSDPEYAKQFPLRKHQIEQINFVTLDSAMKENHGVLDPIVILDKEDASSTAKNIL
                     LYLLRNATEVKIVQTTALTEAISEVISRRENGEVVGFNQVIETLCQSDNQDIVDVGHY
                     FKAIIKNSILELAFSDGNVKGLSYEERVTVLEVADLSLPKDGSDHISDHESNSIALMF
                     ALGAFCKHFGERTNEETVEIFDEAWILMQSSEGKAVIKSMRRVGRSKYNVLMLVSQSV
                     HDAENDDDTTGFGTIFAFYEKSEREAILTHVGLEVNEKNLEWIDNMISGQCLYYDVYG
                     NLNMMSVHNIHPDIDPLLKPMKQSVSSHLENKYAS"
     gene            complement(3409..3819)
                     /locus_tag="VCI33_001551"
     CDS             complement(3409..3819)
                     /locus_tag="VCI33_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP2832464.1"
                     /translation="MEEEKYFDYSRGLNAPYWIQEIKTSKGKLLWYFSTPMQLSFFIV
                     FFLILVLMLTVFSAPMQLLNQLTHSISLLLYWFVPYRLSKFYTEYEPQGKKMHTYLWD
                     YLVYLIDYGFNKKAIYQGERVEVLEEIVFEKTNI"
     gene            complement(3822..4091)
                     /locus_tag="VCI33_001552"
     CDS             complement(3822..4091)
                     /locus_tag="VCI33_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832465.1"
                     /translation="MKVSKVAQVVLTGGGPNVSGLKQFFQGDGIYLISVICAFLVIKN
                     WKNADWLKVGSVLSIYAIVVSLFKGQEILAFLGGMLRWFGIETGL"
     gene            complement(4110..>5004)
                     /locus_tag="VCI33_001553"
     CDS             complement(4110..>5004)
                     /locus_tag="VCI33_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP2832466.1"
                     /translation="GGKQPKPKEVKQKTANIIVYGILGLLFIVGFFGSLRAIGLSNQV
                     NSLKATVLSSKQQNGKDTKEILDIPRVQYYMNNFVYTYINYSDDTATKRKEELDNYYS
                     FSTANLVDDVKKERKLQTQRLVSVEQEEDYHIALMRIGYEVDSKSYQMTLAIPFRMAN
                     GLLAIVSPPYTLAEDLFQGKSKSFERKTVDQVKSLPKQEMLSIQKFLPVFFDKYALSN
                     EIDLKLLMKHPELMGGNYRVKSLDANNARYYQDKSNQVVQLMVTFEDMVTGGTRSENF
                     TLYLIKSDNGWYVDKLYHYFK"
BASE COUNT         1640 a         1036 c          690 g         1638 t
ORIGIN      
        1 tcaaaaattt tcaaaataac ataaatagac ttgatccaaa tatagtcact aacagctgac
       61 atccacctgt tttgttcttc tcctttcaaa gctccgtcac caagtttatt tgtatactct
      121 aatatttgtt gactttttgg tattacaaac tgatttgact tatcatattt tcccagaatt
      181 ttgctattat ctaactcctc ttcttttcct gatttattat tatgaaactt accatttaac
      241 tggccagtat ttacaaaaag ataagctgta taagacgttt tcataacata attatcagct
      301 accgaagcct catgagaaat gatttgcttc tcatcttctg gactcactac cgatagatta
      361 gttactgcat cactaaaaga actagctacc gaatgaacca tatccaacac atagacaccg
      421 cccgaagttg actgcaccgt cccaaagtaa ccgaacgcta ataacataac cgcaaacaaa
      481 tgaagtaatc gtcttgaaaa atcacctttt gaaaaaataa aagctagaaa aatggaaaaa
      541 gcggataagg caattaagag ataaagtaat gaattcgtaa aatctgcgga tttagagagg
      601 ttatcccaca aaactttgga actatcaaac acagtctgac gataagtatt atacaagcta
      661 aagttttcaa aaaatcgtag aataatggaa aagaaaccaa caatcaagtt aagtagaaaa
      721 aagggaaaat taacaaaaat atctgtcaaa gttacttgta accccgtctc aagaccttct
      781 gtactttcca tgtaagcctt taatgcaaaa atatccacat ccttgacttt ctgaaaggta
      841 gaagctatta atccatcatc aatcttatcc atttggcctc tcctttctac gaagcgtatt
      901 tattttctaa gtggcttgaa acagactgtt tcattggttt taataaggga tcaatatctg
      961 gatgaatgtt atggacagac atcatattga ggttgccata aacatcataa taaaggcatt
     1021 gtcctgaaat catgttatca atccactcca ggttcttttc gttcacttcc aaaccaacat
     1081 gagttaaaat agcttcccgt tctgattttt cgtaaaaagc gaaaatagta ccaaaaccag
     1141 tcgtatcgtc atcattttcg gcatcatgta ctgattgaga cactaacatt aaaacattgt
     1201 acttagaacg accaacgcgg cgcatagact tgataacggc cttaccttct gatgactgca
     1261 tcagaatcca agcctcatca aaaatttcaa cagtttcctc attggttcgt tccccaaagt
     1321 gcttacaaaa agcacctaaa gcaaacatta aagcgattga attactttca tgatcagata
     1381 tatgatctga accatcttta ggtaacgata agtccgcaac ttctaaaact gtcacacgct
     1441 cttcatatga taatccttta acattaccat ccgaaaaagc caattccaaa atagaattct
     1501 taataatagc tttaaaataa tgtcctacat ctacaatatc ctgattatct gattgacata
     1561 atgtctcaat gacttgatta aagccaacga cttccccatt ttcacgtctt gagattactt
     1621 cactaatggc ctctgtcaaa gcagtagttt gtacaatctt aacttctgtc gcatttctta
     1681 acagataaag caatatattt ttagcagtag acgaggcatc ttctttatcc aagataacga
     1741 taggatctaa aaccccatgg ttttccttca tcgcactatc caatgtcaca aaattaattt
     1801 gctcaatctg atgttttctc agtggaaatt gtttcgcata ctcgggatca gacaccactt
     1861 gtaaatagtg tttccttaac tctctcttag gatcaatata aagcactcta acattttgtt
     1921 gcgcagtatg taaaaaaatc atctgtgcaa ggtaggactt cccctgacct gtcgcacctg
     1981 taatgataat atgaggattt ttagttactt taccagcaac atcctcctta ttcgcaaccg
     2041 ttgcattaaa caaaactaag ttcttcgagc ccataatcgc ttcaccaata ttatcccaag
     2101 cagataaacg attatcgact cgaccaatat accagccaat cctgttacct gactgggtat
     2161 ttgtaaataa catcatttca ctaaacccac gactggtcac taagtgattc catttacgtg
     2221 ttgtcttctg taaattttga ccataaagta acgtttggaa taaataaggt gtatcatgac
     2281 tagcttcatg tacattgact tgcatatccg caaagtaact taaaacagca tgcctacgtt
     2341 gccttaattg tttgatacta gacccagaaa ccaccaaaaa acaaccgtat tcaatgattt
     2401 cttctttatt tccaactttc ttcatcaagt cttttaatga atatgagccc attagaatat
     2461 catcttgttg cactgtattc gtggagtagg cttctttcat aatttgatca taacgtgtat
     2521 tcgagcgtcc cattgttcca cttaacttag ttttattaat aaattctgct ttaaatcgta
     2581 actcaacagg aaaactcatc cgctgtacga gttcacctaa gtggaaccca ttgaagatag
     2641 tattaaactt cccaactggt agaatcgtta taaatgaact accatacgga ctttctaact
     2701 ttaaaaaacc tcccgacagg ggcttaacta aagtatctgt tacgttcaac aatgttctat
     2761 tggcaataac ttcattttta gcatgtggga tatagcgtaa gtattgcatg cgttgataat
     2821 aaaataaatc ttcttcagat aatctcttag ctttcagtga tgataacaaa ccatacagca
     2881 ataattcttc atcttggtac tctttatacc actccttatc aagttccaca gtgtatccca
     2941 acaaagaggc gacattatct gcaattaaat ccaattgctt taccgcaagt tcttttagtg
     3001 tagcactcat tgtatcccta cctaaattaa caccaataac ccaatcaaat tgataaggta
     3061 tttcaatctc agctgtcaac gtgtcaacag tgtaactcaa tgtatctata cctaaatcct
     3121 taacatcatc tgccaagtca ccaataaaat cagacatttt ttcctctaaa aggtaatctt
     3181 ttggtaccag tgaaatatca aaatgcttgt atttagctaa ttttttgata acctgtgcta
     3241 ccttgtcttt atgactatct tttttatcac tatcagtaat agtgattggc gtatttggaa
     3301 tccgatagta ggcaataacc actttatctt ttcgcaaagc taaattactg tgaatgctat
     3361 taattggata ttccaacttt tttaccatct gatctccttt cttaatcttt aaatgtttgt
     3421 tttctcaaat acaatttctt ctagtacctc tactctttct ccctgataaa tagctttttt
     3481 attaaaacca taatctatta aatagacaag ataatcccaa agataagtat gcatcttttt
     3541 accttgtggc tcatactccg tataaaattt tgaaagtcga tatgggacaa accagtacag
     3601 taacagagaa atagaatgcg ttagctgatt tagcagttgc ataggagccg aaaagactgt
     3661 caacatcaat actaaaatca aaaagaaaac aataaaaaag gaaagctgca ttggtgttga
     3721 gaaataccac aataactttc cttttgacgt ttttatttct tgaatccagt aaggggcgtt
     3781 taggccccta ctgtaatcaa aatatttttc ttcttccatt tttataaacc tgtctcaata
     3841 ccgaaccagc gaagcatccc tcctaaaaag gcaagaattt cttgcccttt aaacagagaa
     3901 accacaatcg cataaattga cagtactgag ccaactttta gccaatctgc atttttccaa
     3961 tttttaatca ctaaaaaagc acagataaca ctaataagat aaatcccatc tccctgaaaa
     4021 aattgtttta gtcctgaaac attaggtcct cctcctgtta aaacaacttg agctactttt
     4081 gatactttca ttaattaaac ctcgcttttt tatttaaaat aatggtacaa cttgtcaaca
     4141 taccaaccat tatctgactt aatcagatac agcgtgaaat tctctgaccg tgtacctcct
     4201 gttaccatat cttcaaaggt aaccattaat tgcacaactt gatttgattt atcttgataa
     4261 taccgagcat tgttagcgtc taaagatttg actcgatagt ttccacccat cagttcagga
     4321 tgcttcatta gtaacttcag atctatttca ttacttaaag catacttatc aaaaaataca
     4381 ggtaaaaatt tttgtattga taacatttct tgcttcggta aactcttgac ttgatccaca
     4441 gtttttcttt caaaactttt tgatttccct tggaaaagat cctccgctaa ggtatatgga
     4501 ggacttacta ttgccaataa tccattagcc atacgaaatg ggatagccaa cgtcatctga
     4561 taagatttac tatctacctc atagcctatt ctcattaaag caatatgata atcctcttct
     4621 tgttctacac taaccagtcg ttgagtctga agcttacgct cctttttaac atcatcaaca
     4681 agattagctg ttgaaaaaga ataataatta tctaattctt ctttacgttt tgtagccgta
     4741 tcatcagaat agttgatgta agtatacaca aaattgttca tgtaatactg cactctagga
     4801 atgtctaaga tttcctttgt atcttttccg ttttgttgct tgcttgataa aaccgtagct
     4861 tttaaactat ttacttgatt tgacaaacca attgccctta atgagccaaa aaatcctaca
     4921 ataaataata atcctaaaat tccatagaca ataatattgg ctgtcttttg ttttacttcc
     4981 tttggtttag gctgctttcc tcct
//
LOCUS       DATUYR010000084         4894 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.084, whole genome shotgun sequence.
ACCESSION   DATUYR010000084 DATUYR010000000
VERSION     DATUYR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.084"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            26..4132
                     /gene="cas9"
                     /locus_tag="VCI33_001554"
     CDS             26..4132
                     /gene="cas9"
                     /locus_tag="VCI33_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEP2832467.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKV
                     LTRSDKNRGKSNNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVRKDLIVKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATFIHQSITGLYETRIDLSQLGGD"
     gene            4132..>4894
                     /gene="cas1"
                     /locus_tag="VCI33_001555"
     CDS             4132..>4894
                     /gene="cas1"
                     /locus_tag="VCI33_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEP2832468.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFS"
BASE COUNT         1684 a          736 c          989 g         1485 t
ORIGIN      
        1 aaacttttta ttttaggagg caaaaatgga taagaaatac tcaataggct tagatatcgg
       61 cacaaatagc gtcggatggg cggtgatcac tgatgattat aaggttccgt ctaaaaagtt
      121 caaggttctg ggaaatacag accgccacag tatcaaaaaa aatcttatag gggctctttt
      181 atttgacagt ggagagacag cggaagcgac tcgtctcaaa cggacagctc gtagaaggta
      241 tacacgtcgg aagaatcgta tttgttatct acaggagatt ttttcaaatg agatggcgaa
      301 agtagatgat agtttctttc atcgacttga agagtctttt ttggtggaag aagacaagaa
      361 gcatgaacgt catcctattt ttggaaatat agtagatgaa gttgcttatc atgagaaata
      421 tccaactatc tatcatctgc gaaaaaaatt ggtagattct actgataaag cggatttgcg
      481 cttaatctat ttggccttag cgcatatgat taagtttcgt ggtcattttt tgattgaggg
      541 agatttaaat cctgataata gtgatgtgga caaactattt atccagttgg tacaaaccta
      601 caatcaatta tttgaagaaa accctattaa cgcaagtgga gtagatgcta aagcgattct
      661 ttctgcacga ttgagtaaat caagacgatt agaaaatctc attgctcagc tccccggtga
      721 gaagaaaaat ggcttatttg ggaatctcat tgctttgtca ttgggtttga cccctaattt
      781 taaatcaaat tttgatttgg cagaagatgc taaattacag ctttcaaaag atacttacga
      841 tgatgattta gataatttat tggcgcaaat tggagatcaa tatgctgatt tgtttttggc
      901 agctaagaat ttatcagatg ctattttact ttcagatatc ctaagattaa atagtgaaat
      961 aactaaggct cccctatcag cttcaatgat taaacgctac gatgaacatc atcaagactt
     1021 gactctttta aaagctttag ttcgacaaca acttccagaa aagtataaag aaatcttttt
     1081 tgatcaatca aaaaacggat atgcaggtta tattgatggg ggagctagcc aagaagaatt
     1141 ttataaattt atcaaaccaa ttttagaaaa aatggatggt actgaggaat tattggcgaa
     1201 actaaatcgt gaagatttgc tgcgcaagca acggaccttt gacaacggct ctattcccca
     1261 tcaaattcac ttgggtgagc tgcatgctat tctgagaaga caagaagact tttatccatt
     1321 tttaaaagac aatcgtgaga agattgaaaa aatcttgact tttcgaattc cttattatgt
     1381 tggtccattg gcgcgtggca atagtcgttt tgcatggatg actcggaagt ctgaagaaac
     1441 aattacccca tggaattttg aagaagttgt cgataaaggt gcttcagctc aatcatttat
     1501 tgaacgcatg acaaactttg ataaaaatct tccaaatgaa aaagtactac caaaacatag
     1561 tttgctttat gagtatttta cggtttataa cgaattgaca aaggtcaaat atgttactga
     1621 gggaatgcga aaaccagcat ttctttcagg tgaacagaag aaagccattg ttgatttact
     1681 cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat
     1741 agaatgtttt gatagtgttg aaatttcagg agttgaagat agatttaatg cttcattagg
     1801 tacctaccat gatttgctaa aaattattaa agataaagat tttttggata atgaagaaaa
     1861 tgaagatatc ttagaggata ttgttttaac attgacctta tttgaagata gggagatgat
     1921 tgaggaaaga cttaaaacat atgctcacct ctttgatgat aaggtgatga aacagcttaa
     1981 acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa ttgattaatg gtattaggga
     2041 taagcaatct ggcaaaacaa tattagattt tttgaaatca gatggttttg ccaatcgcaa
     2101 ttttatgcag ctgatccatg atgatagttt gacatttaaa gaagacattc aaaaagcaca
     2161 agtgtctgga caaggcgata gtttacatga acatattgca aatttagctg gtagccctgc
     2221 tattaaaaaa ggtattttac agactgtaaa agttgttgat gaattggtca aagtaatggg
     2281 gcggcataag ccagaaaata tcgttattga aatggcacgt gaaaatcaga caactcaaaa
     2341 gggccagaaa aattcgcgtg agcgtatgaa acgtattgaa gaaggtatca aagaattagg
     2401 aagtcagatt cttaaagagc atcctgttga aaatactcaa ttgcaaaatg aaaagctcta
     2461 tctctattat ctccaaaatg gaagagacat gtatgtggac caagaattag atattaatcg
     2521 tttaagtgat tatgatgtcg atcacattgt tccacaaagt ttcattaaag acgattcaat
     2581 agacaataag gtcttaacgc gttctgataa aaatcgtggt aaatcgaata acgttccaag
     2641 tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg ccaagttaat
     2701 cactcaacgt aagtttgata atttaacgaa agctgaacgt ggaggtttga gtgaacttga
     2761 taaagctggt tttatcaaac gccaattggt tgaaactcgc caaatcacta agcatgtggc
     2821 acaaattttg gatagtcgca tgaatactaa atacgatgaa aatgataaac ttattcgaga
     2881 ggttaaagtg attaccttaa aatctaaatt agtttctgac ttccgaaaag atttccaatt
     2941 ctataaagta cgtgagatta acaattacca tcatgcccat gatgcgtatc ttaatgccgt
     3001 cgttggaact gctttgatta agaaatatcc aaaacttgaa tcggagtttg tctatggtga
     3061 ttataaagtt tatgatgttc gtaaaatgat tgctaagtct gagcaggaaa taggcaaagc
     3121 aaccgcaaaa tatttctttt actctaatat catgaacttc ttcaaaacag aaattacact
     3181 tgcaaatgga gagattcgca aacgccctct aatcgaaact aatggggaaa ctggagaaat
     3241 tgtctgggat aaagggcgag attttgccac agtgcgcaaa gtattgtcca tgccccaagt
     3301 caatattgtc aagaaaacag aagtacagac aggcggattc tccaaggagt caattttacc
     3361 aaaaagaaat tcggacaagc ttattgctcg taaaaaagac tgggatccaa aaaaatatgg
     3421 tggttttgat agtccaacgg tagcttattc agtcctagtg gttgctaagg tggaaaaagg
     3481 gaaatcgaag aagttaaaat ccgttaaaga gttactaggg atcacaatta tggaaagaag
     3541 ttcctttgaa aaaaatccga ttgacttttt agaagctaaa ggatataagg aagttagaaa
     3601 agacttaatc gttaaactac ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg
     3661 gatgctggct agtgccggag aattacaaaa aggaaatgag ctggctctgc caagcaaata
     3721 tgtgaatttt ttatatttag ctagtcatta tgaaaagttg aagggtagtc cagaagataa
     3781 cgaacaaaaa caattgtttg tggagcagca taagcattat ttagatgaga ttattgagca
     3841 aatcagtgaa ttttctaagc gtgttatttt agcagatgcc aatttagata aagttcttag
     3901 tgcatataac aaacatagag acaaaccaat acgtgaacaa gcagaaaata ttattcattt
     3961 atttacgttg acgaatcttg gagctcccgc tgcttttaaa tattttgata caacaattga
     4021 tcgtaaacga tatacgtcta caaaagaagt tttagatgcc acttttatcc atcaatccat
     4081 cactggtctt tatgaaacac gcattgattt gagtcagcta ggaggtgact gatggctggt
     4141 tggcgtactg ttgtggtaaa tacccactcg aaattatcct ataagaataa tcatctgatt
     4201 tttaaggatg cctataaaac ggagctgatc catttatcag aaattgatat tttgttatta
     4261 gaaacgaccg atattgtctt gtccactatg ctggtaaaac ggctagtgga tgagaatgtc
     4321 cttgtcatat tctgtgatga taaacgatta ccaacagcta tgctgatgcc tttttatggt
     4381 cgtcatgatt cgagtttaca gcttgggaaa caaatgtcct ggtcagaaac agtcaaatcg
     4441 caggtttgga cgacgattat tgctcaaaag attttgaatc aatcttgcta tctaggagca
     4501 tgctcctatt ttgaaaaatc ccaatctatt atggatttat atcatggttt ggaaaatttt
     4561 gatccgagta atcgagaagg gcatgcagcg agaatttatt ttaatacact ttttgggaac
     4621 gatttctcaa gagatttgga gcatccaatc aatgcaggtc tggattatgg ttatacttta
     4681 ttattgagta tgtttgcgcg tgaagtggtt gtgtctggat gtatgactca gtttgggctt
     4741 aaacacgcta atcagtttaa tcagttcaat tttgctagcg atattatgga accatttagg
     4801 cctttggtgg ataagattgt ttatgaaaat cgaaatcagc cttttcccaa aataaagaga
     4861 gagttattta ctttgttttc agatacattt tcat
//
LOCUS       DATUYR010000085         4712 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.085, whole genome shotgun sequence.
ACCESSION   DATUYR010000085 DATUYR010000000
VERSION     DATUYR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.085"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1172
                     /locus_tag="VCI33_001556"
     CDS             <1..1172
                     /locus_tag="VCI33_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP2832469.1"
                     /translation="LVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTEQKAAQNQVS
                     ALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLKNQARSAYKN
                     NETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEEKQAANQTAI
                     NTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQKEAAEKAAAE
                     ALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPAALTAPAAPS
                     AGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYRTGSTPMVGA
                     IAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGVTFIYPH"
     gene            1425..2387
                     /locus_tag="VCI33_001557"
     CDS             1425..2387
                     /locus_tag="VCI33_001557"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP2832470.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMDLQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            2573..3328
                     /gene="recO"
                     /locus_tag="VCI33_001558"
     CDS             2573..3328
                     /gene="recO"
                     /locus_tag="VCI33_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEP2832471.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            3431..4438
                     /gene="plsX"
                     /locus_tag="VCI33_001559"
     CDS             3431..4438
                     /gene="plsX"
                     /locus_tag="VCI33_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEP2832472.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMRKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            4431..4673
                     /locus_tag="VCI33_001560"
     CDS             4431..4673
                     /locus_tag="VCI33_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP2832473.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHLAIPDEDVEDMVFMGDVLDYLVQRLT"
BASE COUNT         1522 a          740 c          978 g         1472 t
ORIGIN      
        1 ttcttgtaag tggtgttacc ctcggagcag ctacaactgt aggagcggag gatttaagta
       61 ctaagattgc taagcaagat tctattatct caaatctgac tacagagcaa aaagctgcac
      121 agaatcaagt ttcagcgtta caggctcaag taagttcact acaatctgaa caagataaac
      181 tgaccgcaag aaatacagaa cttgaggcgc tttcaaagcg atttgagcaa gaaattaagg
      241 ctctaacaag tcaaattgtt gctcgtaatg aaaaattaaa aaatcaagct cgtagtgctt
      301 ataaaaacaa tgaaacttct ggttatatta atgcactttt gaattctaaa tcaatttctg
      361 atgttgtaaa ccgtttagta gcaattaata gagctgtctc tgctaacgct aaattgttag
      421 aacaacaaaa agctgataaa gtttcccttg aagaaaagca agctgctaac caaacagcta
      481 ttaataccat tgccgctaat atggcaatgg ctgaagaaaa ccaaaataca ttacgtactc
      541 aacaagctaa tttggtagct gcaactgcaa atttagctct ccaattagca tctgctactg
      601 aagataaagc taatttggta gctcaaaaag aagctgcaga aaaagctgct gctgaagcct
      661 tagcacaaga acaggctgct aaagttaagg cacaagaaca ggctgcacaa caagcagcat
      721 ctgttgaagc agcaaaatct gctattactc cagcaccaca agctactccg gcagcgcaaa
      781 gtagtaatgc tattgaacca gctgcactca cggctccggc agctccttct gcaggaccac
      841 aaacatcata tgattcttct aatacttatc cagttggaca atgcacatgg ggagctaaat
      901 ctttagctcc ttgggcagga aataattggg gaaatggtgg tcaatgggct tatagtgctc
      961 aagcagctgg ttatcgtact ggttcaacgc cgatggtagg tgcgattgcc gtttggaacg
     1021 atggtggtta tggacatgtc gccgttgtag ttgaggttca aagtgcctca agtattcgtg
     1081 tgatggagtc taactacagt ggtagacagt acattgctga ccaccgtggc tggtttaatc
     1141 caacaggtgt tacatttatt tatccacact aacatattat ttaggttaga acagttgttc
     1201 taaccttttt gattactcct attgtggaat agttctgaga gtattgacga tatagttaat
     1261 ttataagaga gttgtttggc caatcgctta tgtataatat aagagaagta tataaagtcg
     1321 ataaaaggta tatttaatac attctttaac ggtttttcaa tttcatttga taattcactt
     1381 gaaaagcgtt aaaatggtat aggattaaaa ctttggagga aaaaatgtct tattctgatt
     1441 taaaattgtt tgctttatcg tcaaacaagg aattagcaga aaaagttgcg tctgcaatgg
     1501 atcttcaact tggaaagtct acggttcgcc agttttcaga tggtgaaatc caagtaaata
     1561 ttgaagaatc aattcgtggt catcatgtct ttattttgca atcaactagc tcacctgtca
     1621 atgacaattt aatggaaatt ttgattatgg tcgatgcttt aaagcgtgct agtgctgaaa
     1681 aaatttcagt tgtgatgcct tactacggct atgcacgcca agaccgtaaa gcgcgttctc
     1741 gtgaacctat tacttcgaaa ttagttgcta atatgttaga agttgctggt gtagatcgct
     1801 tattaacggt tgatttacat gctgctcaaa ttcaagggtt ctttgatatt ccagttgatc
     1861 acttgatggg tgcaccttta attgcagact attttgatcg tcatggttta gtgggtgaag
     1921 atgttgtcgt tgttagtcct gaccatggtg gtgtgactcg tgcgcgtaaa ttagcacaat
     1981 tcctccaaac accaattgca attatcgata agcgtcgtag tgtggataag atgaacacca
     2041 gtgaagtgat gaatattatc ggaaatgttt cgggcaagaa atgtatcttg attgacgata
     2101 tgattgatac cgcagggact atttgccatg cagcagatgc gcttgctgaa gcgggggcaa
     2161 cagctgtgta tgcgtcatgt acgcatcccg ttctttctgg accagcactt gataatattc
     2221 aacgttcggc tatcgaaaaa ctaattgttc ttgatactat ttatctgcca aaagaacgcc
     2281 ttattgataa aattgaacaa atttcaattg ctgatttggt tgcagaagcg attattcgca
     2341 ttcatgaaaa acgtccgttg tcacctttat ttgagatggg caattaaact taacgaagcc
     2401 tgagataata gatctcaggc tttttgactt gaataaaatg taagggtaat gtggtattaa
     2461 ttattgtggg atgaactatg caattctaaa ggattttggg tataataaga aagataacaa
     2521 cagtttattg taaactaaga taaaaggtaa gagaaaaata cgacgaggta atatgcaact
     2581 aacagaatca ctaggcatcg ttctttttaa taggaattat cgagaagatg ataaattagt
     2641 caaaatattt actgaagtag caggtaagca gatgtttttc gtgaaacata ttagtcgttc
     2701 caaaatgtcc tcaatcattc aaccactaac gattgctgat tttattttca agttaaatga
     2761 tacaggcttg tcttatgttg ttgactatag taacgttaac acttatcggt atattaataa
     2821 tgatattttt cgattagcct atgctagtta tgtcttagca ttagctgatg ctgcgattgc
     2881 agataatgaa tcagattcgc atttgtttac gtttttgaaa aaaacacttg atttgatgga
     2941 agagggccta gattatgaaa ttttgacaaa tatttttgaa attcagatat tagatcgttt
     3001 tggtattagt ctaaactttc atgagtgtgc catttgtcat cgcactgatt taccgcttga
     3061 tttttcccat cgtttttcag ctgtactttg ttctgaacat tattacaaag acaaccgacg
     3121 taatcattta gatccaaatg ttatctactt gttgagtcga tttcaaaaaa tcacatttga
     3181 tgatttgaga actatttcat tgaataaaga catcaaaaag aagcttcgtc agttcattga
     3241 tgagttgtat cacgactatg taggaatcaa attaaaaagt aaaacattca ttgataattt
     3301 agttaagtgg ggagatatta tgaaatgata gggcctcttt cgtttatggg aattggtttc
     3361 agaatatgct ataataaaaa tactattaat agattgtgta agaacacaag atataattaa
     3421 aggagtggaa atgaagagaa ttgcaataga tgctatgggt ggagataacg ctcctaaagc
     3481 tattgttgag ggtgttaatc aagcaataga agcattttca gatatcgaaa ttcaacttta
     3541 cggtgatcag acaaagatta actcctatct tattcaatca gatcgtgttg ctattattca
     3601 tacagatgaa aaaattatgt cagatgatga acctgcaaaa gctgttcgcc gtaaaaagaa
     3661 ggcttcaatg gttttagcag ctaaggcagt gaaagaagga aaagcagatg ccattatttc
     3721 agcaggtaat acaggtgctc ttttagctgt aggtttgttt gttgtggggc gtatcaaggg
     3781 agttgatcgt ccaggattat tatcaactat tcccacagta accggattag gatttgatat
     3841 gttggatctt ggagctaatg ctgaaaatac ggcaaaacat ttacatcaat atgctatttt
     3901 aggctctttc tatgctaaga atgtacgtgg gattgctaat ccacgtgttg gtctattaaa
     3961 caatggtaca gaagaaacaa aaggagatcc ccttcgtaag gccacttatg aacttttaac
     4021 agcagataat acaatatctt ttgttggtaa tgtcgaggcg cgagaactta tgtcaggtgt
     4081 tgcagatgtt attgtatcag atggtttcac gggaaacgca gtgttgaaat caatagaagg
     4141 aacagctatt agcattatgg ggcaactgaa acagataatt aatagcggcg gtattaagac
     4201 taaaattgga gctagtctac ttaaaagtag tctttacgag atgaggaaga ctcttgacta
     4261 ctctagtgct ggaggtgcag ttttatttgg tttaaaggca cctgttgtaa agtctcatgg
     4321 ttctagtgat gtaaaagcta ttttttcaac gataaaacaa gtacgtacga tgctagacac
     4381 taacgtggtc ggacaattag tagaagaatt tgctaaggag actcaagtaa atgactagac
     4441 aggagatttt tgaaagactg attaacttaa ttcagaagca aagaagctat ttgagtgtag
     4501 ctattacaga acaaacacat ttaaaaaatg atttagcggt tgactctatt gaactagttg
     4561 aatttattat taatgttgaa gatgaatttc atcttgctat tcctgatgaa gatgttgagg
     4621 atatggtatt tatgggagac gttttagatt atttagttca gcgtttgact tagttagaat
     4681 aataaaaaga gcgtaagctc tttttattat tc
//
LOCUS       DATUYR010000086         4706 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.086, whole genome shotgun sequence.
ACCESSION   DATUYR010000086 DATUYR010000000
VERSION     DATUYR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.086"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            63..1385
                     /gene="ska"
                     /locus_tag="VCI33_001561"
     CDS             63..1385
                     /gene="ska"
                     /locus_tag="VCI33_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEP2832474.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSQPAHGGKTEQGLSPKSKPFATNSSAMPHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGNLDFTPSLKEQYHLTTLAVGD
                     SLSSQELAAIAQFILSKKHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYGINKKSGQEEKMNNTDLISEKYYILKKGEKPYDPFDRSHLKLFTINYVDVNTNKLL
                     KSEQLLTASEHNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHNDTNRIIT
                     VYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK"
     gene            complement(1483..1926)
                     /locus_tag="VCI33_001562"
     CDS             complement(1483..1926)
                     /locus_tag="VCI33_001562"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEP2832475.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVPVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(1941..4160)
                     /locus_tag="VCI33_001563"
     CDS             complement(1941..4160)
                     /locus_tag="VCI33_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEP2832476.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKTDVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLMHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(4412..4522)
                     /locus_tag="VCI33_001564"
                     /pseudo
     CDS             complement(4412..4522)
                     /locus_tag="VCI33_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129544913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT         1310 a         1095 c          916 g         1385 t
ORIGIN      
        1 tctataaaat tattatgtta ttctaataat ggggattgaa acttaacttt taggaggttt
       61 ctatgaaaaa ttacttatct tttgggatgt ttgcactgct gtttgcatta acatttggaa
      121 cagtcaagcc tgtccaagct attgctggac ctgaatggct gctagaccgt ccatctgtca
      181 acaacagcca attagttgtt agcgttgctg gtactgttga ggggacgaat caagaaatta
      241 gccttaaatt ttttgaaatt gacctaacat cacaacctgc tcatggagga aagacagagc
      301 aaggcttaag tccaaaatca aaaccatttg ctacaaatag tagcgcaatg ccacataaac
      361 ttgaaaaagc tgacttatta aaggctattc aagaacaatt gatcgctaac gttcacagta
      421 acgacggcta ctttgaggtc attgattttg caagcgatgc aaccattact gatcgaaacg
      481 gcaaggtcta ctttgctgac cgagatgatt cggtaacctt gccgacccaa cctgtccaag
      541 aatttttgct aagcgggcat gtgcgcgtta gaccgtatca acctaaagcc gttcacaact
      601 cagctgaacg cgttaacgtc aactatgaag tgagctttgt ctccgaaaca ggaaatttag
      661 actttacgcc atcgttaaaa gagcaatacc atttgaccac actggcagtt ggtgactctc
      721 tttcatcaca agagttagca gcaattgccc aatttatcct atcaaaaaaa catccagatt
      781 atatcattac aaaacgtgac tcctcaatcg tcactcatga caatgacatt ttccgtacga
      841 ttttaccaat ggatcaagag tttacttacc atatcaaaga ccgggaacaa gcttatggga
      901 tcaataaaaa atctggtcag gaagaaaaaa tgaacaacac tgaccttatc tctgagaaat
      961 attacatcct taaaaaaggg gaaaagccgt atgatccctt tgatcgcagt cacttgaaac
     1021 tgttcaccat caattacgtt gatgtcaaca ccaacaaatt gctaaaaagc gagcagctct
     1081 taacagctag cgaacataac ttagacttca gagatttata cgatcctcgt gataaggcta
     1141 aactactcta caacaatctc gatgcttttg gtattatgga ctatacctta actggaaaag
     1201 tagaggataa tcacaatgac accaaccgta tcataaccgt ttatatgggc aagcgacccg
     1261 aaggagagaa tgctagctat catttagctt atgataaaga tcgttatacc gaagaagaac
     1321 gagaagttta cagctacctg cgttatacag ggacacctat acctgataac cctaacgaca
     1381 aataaccacg atcttctaaa acgatgagat taactgacaa aaaaagcaag caacacgcta
     1441 tcaacagttg cttgcttttt tctaacctct tagttgtaga gactagtgac atttcgtgtc
     1501 taaaataatc gtaactggtc catcattgat gagactaacc tgcatatctg ccccaaaaac
     1561 gccacgctca actggcacaa actctgccaa ttgttcatta aagctatcat acaactggct
     1621 agccaaatct ggcttagcag caccagtaaa ggcaggacgg tttccctttt tggtgtcagc
     1681 ataaagggta aattgcgaca cagataagat actgcccttg atgtcttgga tagactgatt
     1741 catcttgcca tcagcatctg aaaaaatgcg catgttgact atttttcgca cagcgtaagc
     1801 caaatcttct gcattatcat ctggtcccac acctactaac aacaacaacc cttggttaat
     1861 agcgccagca attttcccat caatagaaac agaggcctct ttgacccgtt gcaagacaag
     1921 tttcatggtt ttatcctttc ttagccattg gtccgcttca cgctataaac atctggaact
     1981 gccttgattt tttcgacaac agtggtcaga tgcatcagat ttggaatgcc aaagctcacg
     2041 tgaatattag caaacttcat gtccttggtc ggctgagcat tgactgtcga tatgctcttg
     2101 gttgagtttg ataaaatttg gagcacatca ttgagcaggc cactacgatt gagcccatag
     2161 atatcaattt cagcctgata atctttactc gaattgtcca aatcccactc gacctcaatc
     2221 aagcgttctt ggtagccatc ttgactctta atgttatgac agtccgatct gtgaatcgca
     2281 atgccacgcc ctttggtaat atagccgtca ataggatcac caggtacagg attacaacac
     2341 ttggcaatcc gcatcaagag gcctgatgct ccttggataa tgaccccatt ttcactgcga
     2401 accttgagca catctttgtt ttcgtgtttg acctcaccgc ccttaaccaa ttcttcagct
     2461 tctgctttag ccttggccct ttcttcttca cggcgctctt tttcggttaa cttgttaaag
     2521 acactgatag gactaatgtc accaaaccca acggctgcat agagtgattc ttcgctcttc
     2581 acactgactt ttggaaggat cgcttcaatg cgttttttgt caaggtattt attggcaaca
     2641 tagccctgct cttgaaaata agacaccaac aaatcacggc ctttattcac tgacaattcc
     2701 ttgtcttgat ttttaaagaa ctgacgaatt ttgttgcgag ccttattggt tttgaccagt
     2761 tttacccagt ctcgactagg gccaaaggaa ttggcattgg tgatgatttc aaccacatct
     2821 cctgttttta acttggcagt gagaggaacc atacgtccat tgactttggc acctgttgct
     2881 ttttcaccga tttgcgtatg gatcgcataa gcaaaatcaa taggacctga ttcttttggc
     2941 aactcctgaa cggcccctgt cggtgtaaag acataaatcc gttcagaaaa aatgtcttct
     3001 ttgaccgaat ccacaaagtc cactgcatcg ccatttgagg catcttgcaa ttctaccagc
     3061 tctttgatcc agttcatgcc aacggcttgc tcagcctgat tgaccttacc acgcacgcct
     3121 tttttataag cccagtgagc agcaaccccg tactcagcca cttgatgcat gtccttagtt
     3181 ctgatttgaa tctcaatagg tccttttggt ccatacacag tggtatgaat cgactggtag
     3241 ccattagctt taggagctgc aatataatcc ttgaagcggc ctggcatggg acgccaaagc
     3301 tcatgaatat agccaaccat agcataaaca tcgctctgcg tttccatgac acaacggata
     3361 gcaatcaaat caaaaatctg atcgaaacgt ttctttttgt cccgcatttt ccgataaatc
     3421 gaataaatgt gttttggtcg gccatacaca tctccaaaca acccttgttg tgtcgtatag
     3481 gttttgacct tactgacaat agcctctacc aaagcttcac gctcgcgacg tttttctttc
     3541 atcatatggg aaattttgta aaattcggtt tcattgaggt aacgaaaagc caaatcttct
     3601 agttcccatt tgatgcgact aatccccaaa cgatgcgcca agggggcata gatttccatg
     3661 gtttcgcgcg aaatgcgctc ttgtttgtcc ttgcgcaaat gtttgagggt gcgcatatta
     3721 tgcaggcggt cagccaattt caccaaaatc acgcgaatat ctttagacat agccatcagc
     3781 attttgcgat ggttttcggc gagttgctcc tcatgagatt tgtactcaac tttacctaac
     3841 ttggtgacac catcaacgat atcacgagca tcttggccaa agtctgcttc gatctcatct
     3901 aaggtaatat ccgtatcttc tacgacatca tgtaaaaagc cacaggcaac tgtcacagca
     3961 tccagatgca aatcagccag aatccccgcc acctgaatcg gatggacgat atagggttcg
     4021 cctgactttc tcacttggta gaaatgggcc gctgttgcat atgctaaagc ctttgccaca
     4081 aaagccacat cagtcttggt catataggtg gccgctaagg caatgacttc ttctcctgtt
     4141 acgttcatta tttttgccat ttcgttcctc atctagcatt tcttttccct attttaacac
     4201 tttacacaca aaagcaaaag gcagaatctg ctaagaaagc gcttagtgtg accttttctt
     4261 agaaaacggt tagtgagctg cttttgatat cttttgggat ctctcaggcg tgagcatgat
     4321 accaacggcc agctcccgac caaaaataga gagaaccaag gggctctctc tattatgttt
     4381 gcttatcagt aaggtctctc aattcagagg attagttgtt ttctttgcgt tttgtaacgg
     4441 ctaggacacc agctgttgtc atcactgcta ctgctgctgc tgtaaagaat gggttggctt
     4501 gctcaccagt tgctggtaga cgtctgtggt tgttggctac aggtgtctta gccatattgc
     4561 cattgcggcg ttctggcgtt tggttagctt ctttgtcagc aggtttttct ggagctggag
     4621 ttacctcttt gctcttttct ggagcttttt cacctggttg ctctggcatt tctggcgttg
     4681 gtgtcacttc tgggctcttt tctgga
//
LOCUS       DATUYR010000087         4615 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.087, whole genome shotgun sequence.
ACCESSION   DATUYR010000087 DATUYR010000000
VERSION     DATUYR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.087"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(52..813)
                     /locus_tag="VCI33_001565"
     CDS             complement(52..813)
                     /locus_tag="VCI33_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP2832477.1"
                     /translation="MTKEKMMSFMTIASIALLGSGVVCANDVADPVAGTTDAVVVPVS
                     PQPTTPIEHTGTDTTNKIVDSTKPSEDKVADSTDQLIPPVKSDLETSEDKVTDPTETK
                     PSKEKTEEPVKSNDSEVIPEQPKSEEVKKAEENTGASTTETPKPVTKPVIETPIVTNT
                     GYTVLGTQDGNVWVQTETGNVLKPAIAIGAVKQKDGTVALKTRDGKLEVLPSTGETKT
                     ILAILGGLAILGAIWVGWKENIKKYLTFLKKKSKK"
     gene            complement(838..1395)
                     /locus_tag="VCI33_001566"
     CDS             complement(838..1395)
                     /locus_tag="VCI33_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832478.1"
                     /translation="MKIRKVIIVMLAVALSLGFYRLYHRQKPSQLIEANQEILVKPIT
                     PNKSKIKVKRNVAKEAKATLEMPEERVNEKEAKQVKSAIVIPIYYLASISSQDDIKVT
                     YDNKLSITNPAMAASIKTMLLAGYHFDLDSLTVYQSNSDNVYQFTILLVTHQQDQLSL
                     VGNYVTGTGQFEFVSLHGTPKNVMF"
     gene            complement(1395..2747)
                     /locus_tag="VCI33_001567"
     CDS             complement(1395..2747)
                     /locus_tag="VCI33_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP2832479.1"
                     /translation="MSIPFFIVVIVTIIFSSSQGDCQVTPDLSTQFVTSSDSASQTDW
                     TKKGSIANVTAGKVFHAWVSKGLSGASAAGIVGWVNSEGGFAMIGRAEGHYGNDLRTN
                     SIAFGVKPIGLSYYTTEAGGGIYQFTPYTKYAPLGDPKWENADAMNEFVAKAILAGDW
                     NASMDLTGGNHTFKQMAQMTDPKQATLVWQAYERGSTAHINPFQKQADAQKAYELFGG
                     VKYSYNEIVFHKSFDIFGPLKPGEGESKEVVLSLCESGSYSKGAFGKDRTGKVNYTSY
                     NAWRPEQLPADLKPYALDPRSVGLNYRQAVGWYAIASTGGQCTDLSASLMYALWSKNG
                     SHPTQKAGNGNMVVANWVRTFGGSSDKSPTSGAVFSSAGTSSAGHTGVVSHVFENGDI
                     LIVEQNYASYSGDDGGFGKYSWNYRYVTTAELKNEGYTFYNPAKLGYQIMKSVGTVGK
                     "
     gene            2888..3031
                     /locus_tag="VCI33_001568"
     CDS             2888..3031
                     /locus_tag="VCI33_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP2832480.1"
                     /translation="MGNSSKSNRKEELDLSTFEVLTLMFIAGNFVIALVKLVLELVKS
                     TKK"
     gene            complement(3199..3768)
                     /locus_tag="VCI33_001569"
     CDS             complement(3199..3768)
                     /locus_tag="VCI33_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832481.1"
                     /translation="MGKHSPMNFSKPSQMTESIDHDVHYDLPGVNEEMESPFQSESDY
                     QSQNSSIIVETPEMDDMIYQSSQKKNISIGNNYSSDKKSLENSFDSPIIPERSEPETD
                     AKERFNPQAEYQRLKAKRQKPLGNRKKSKLEAELGQFTDDASYYKAHGGTAFKRGFNA
                     SKSKELRLKQNLERKATILAELDKLRGHV"
BASE COUNT         1371 a          974 c          741 g         1529 t
ORIGIN      
        1 tttcgctcct ttcaacaaaa ctaaagccat gaaattaatc atggctttat cttatttttt
       61 acttttcttt ttcaaaaacg ttaaatactt tttgatattt tctttccaac ctacccagat
      121 agctcctaaa atagctaaac ctcccaaaat ggctaagatt gttttagtct ctccagttga
      181 tggtaagact tctaacttcc catctcttgt ttttaaggca actgtaccat ctttttgctt
      241 tacagcacca attgctatcg caggtttcag tacattacca gtttctgttt gaacccaaac
      301 attgccatct tgcgttccca aaaccgtgta gcctgtattc gtaacaattg gtgtctcaat
      361 aacaggtttc gtcacaggtt taggagtttc agttgtacta gcacctgtat tttcttcagc
      421 ttttttcacc tcttctgact taggttgttc tggaatcact tctgaatcat tggacttaac
      481 aggctcttcc gtcttctctt tgcttggctt agtctccgtt ggatctgtta ctttatcctc
      541 gcttgtttct aaatctgatt taactggagg gattagctga tcagtactat ctgccacctt
      601 gtcctcgctt ggcttagtcg agtcaacaat tttattggta gtatctgttc ctgtatgctc
      661 aatcggtgtc gttggttgag gtgagactgg taccaccact gcatccgttg ttcctgctac
      721 tggatctgct acatcattag cacaaaccac cccacttcca agcaaagcaa tacttgcaat
      781 cgtcataaaa ctcatcattt tttcttttgt catcttattt ttcctctttt cgtttaatta
      841 aaacatgaca ttttttggag tgccatgtaa actcacaaat tcaaactgac ccgtaccagt
      901 aacataatta ccaacaagac ttaattgatc ttgctgatga gtcactaata aaatcgtaaa
      961 ttgatacaca ttatcagaat tgctctgata aaccgtcaag ctatctaaat caaagtgata
     1021 ccctgctaaa agcatcgttt taatgcttgc tgccattgct ggattagtaa tggacaactt
     1081 attatcataa gtaaccttaa tatcatcttg acttgatata ctagctaaat aataaatcgg
     1141 tattacaata gctgatttca cctgtttagc ttctttttcg ttaacacgct cctcaggcat
     1201 ctctaaagtg gccttggctt ctttggcaac atttctctta accttaattt tacttttatt
     1261 cggtgtaatt ggttttacaa gtatttcttg attagcttct attaactgag atggcttttg
     1321 acgatgatac aaccgataaa atcctaatga aagagctact gccagcataa caataataac
     1381 ttttcttatc ttcattattt acctaccgtt cctactgatt tcataatctg ataaccgagt
     1441 ttcgcaggat tgtaaaaagt atatccttca ttttttaatt ctgcagtagt gacatagcga
     1501 tagttccaag agtatttccc aaagccacca tcatcacccg aataagatgc ataattttgt
     1561 tccacaatta aaatatcccc attttcaaaa acatggctga caacacctgt atggccagca
     1621 gaactagtcc ctgcagagga aaatacagct cctgaagttg gtgatttatc agatgaaccg
     1681 ccaaaagtcc taacccaatt agcaacaacc atattaccat tacctgcctt ttgtgttgga
     1741 tggctaccat tttttgacca caaagcatac atcaaactcg cagaaagatc ggtacattga
     1801 ccccctgtcg aagcaatcgc ataccagcca accgcttgtc gataattcaa gccaactgat
     1861 ctgggatcga gcgcataagg ttttaaatct gcaggcaatt gttctggtcg ccaagcattg
     1921 taggaagtat agttgacttt gcctgtacga tctttcccaa aagccccctt agagtaagaa
     1981 cctgattcgc ataatgataa aacgacttct tttgattccc cttcccccgg ctttagtgga
     2041 ccaaaaatgt caaaagattt atgaaatact atttcattat aactatattt aactccaccg
     2101 aataactcat acgctttttg agcatctgct tgcttttgaa atggattaat atgggcagta
     2161 ctaccacgtt cataagcttg ccaaactagc gtagcctgtt tcggatcggt catctgggcc
     2221 atttgcttaa aggtatgatt tcctcctgtc aaatccattg aagcattcca atcaccagct
     2281 agaatggctt ttgcaacaaa ttcgttcatg gcatccgcat tctcccactt aggatcacct
     2341 aaaggggcat atttggtata aggggtaaat tgataaatac ctccacctgc ttctgtcgta
     2401 tagtaactca aaccaattgg tttcactcca aaagctatag aattagttcg taaatcatta
     2461 ccataatgtc cttctgctcg acctatcatg gcaaaaccac cttcggaatt aacccaacct
     2521 acaattcctg cggcagaagc gcctgacaag cctttcgata cccaagcgtg aaagacttta
     2581 cctgctgtga cattcgcaat tgagcctttc tttgtccaat cggtttgtga agcagaatca
     2641 cttgaggtaa caaattgtgt tgataaatca ggagttactt gacaatcacc ttgactgctg
     2701 ctaaatatga tcgtaacgat aactacaata aaaaaaggaa tgctcataag cactcctaga
     2761 catccaagtt tcttcttgat aacattccct ccttcttaaa ctggtcagac agagatacgg
     2821 tatgttataa taaaaaggca gaaaagttaa gacggtggct cttatttctg aaaggtaggt
     2881 ggtgcccatg ggcaattcat caaaatccaa cagaaaggag gagctagatt tgtctacctt
     2941 tgaggtctta acactcatgt ttatagcagg taacttcgtt atcgctctcg tgaagttagt
     3001 ccttgaactg gtaaaatcga caaaaaaata accgtctcaa ctttggcgag ttaacggtta
     3061 ttttttaacg gttattttct gccaccgtct taaacggttc tgcattgggt agttgtttcc
     3121 gcaactgccc ttttctattt tcattataac tcttatctcc aaattaagca actatttgat
     3181 aacatcacaa tacttccttt aaacatgtcc tctaagttta tctaattcag ctaaaatagt
     3241 cgcctttctt tctaaatttt gttttaaccg taactctttt gatttactgg cgttaaatcc
     3301 tcttttaaag gcagtccctc catgtgcttt ataatagctg gcatcatcag taaactgacc
     3361 taattctgct tctaatttag attttttccg atttcctaaa ggtttttgtc gcttagcttt
     3421 tagacgttga tattctgctt gaggattaaa tctctcttta gcgtccgttt ctggttcaga
     3481 cctttcagga ataattggag aatcaaatga attttccaaa gattttttat cagaggaata
     3541 attatttcca atagaaatat ttttcttctg actgctttga taaatcatat catccatttc
     3601 tggagtttca acgataattg aagagttttg agattgataa tcactttctg actgaaatgg
     3661 actttccatc tcctcattaa cacctggtaa atcataatga acatcatgat caattgattc
     3721 agtcatctga cttggtttag aaaaattcat tggggaatgt ttacccaagg gttctgaatt
     3781 atctaatgaa gctccaaaag attggctgta taagttgtct ctttcaaaat cacttacttg
     3841 agagagattg taatcagaag aaaagttact ctcttgatta tcgaaaacac cttgttctgc
     3901 ttgatgtcca gaggtaggat taccattatc agaaacatgc gggacactat ttgtggaaaa
     3961 ttcttgatct cctgcaaaat ctcctaattc tgaaaactga tggtcgaaat tagtttcatt
     4021 agaaaggtca ctataattag aagtgtcatc tcctttgaaa cggtttttaa tggcactaag
     4081 attatcctta gcatgtacaa ggctaccaac accataacca gctcccatca tcgcaagatt
     4141 agaaatatca tgcgccttat catagatatt ttggggtgta actcctaaac tattcgcttt
     4201 ttgagcaacc atatttcctg cttgtgtgac tgcttgtgca gcgtctgctc ctacaattaa
     4261 acttaatacc ttttctttat gtttccaaat ttgccaaaaa ataaccccct gaacaacaat
     4321 agtaataaac aacataaata aaatagattg tcccttaagg ttactgctgc ttaacaaatg
     4381 attgtctgta aatcgagact taataaatgt cgcaatcaat gactgtatat aaaagactat
     4441 taaagttaaa aaacctgtta gagctggaaa ggaaacacct ccaagcatta gcttaagcat
     4501 attaaaagca atatgttgca ttttaggtaa aaaggacacc aataaagcca gtgggaataa
     4561 aaacattaac aatatgacca caatatctgc taaaaaagca attaattgta tcaat
//
LOCUS       DATUYR010000088         4514 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.088, whole genome shotgun sequence.
ACCESSION   DATUYR010000088 DATUYR010000000
VERSION     DATUYR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.088"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..1137)
                     /locus_tag="VCI33_001570"
     CDS             complement(<1..1137)
                     /locus_tag="VCI33_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEP2832482.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYNTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKKPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPYFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYR"
     gene            1227..2093
                     /locus_tag="VCI33_001571"
     CDS             1227..2093
                     /locus_tag="VCI33_001571"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEP2832483.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            2071..2628
                     /locus_tag="VCI33_001572"
     CDS             2071..2628
                     /locus_tag="VCI33_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2832484.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRIPIGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            2762..4306
                     /locus_tag="VCI33_001573"
     CDS             2762..4306
                     /locus_tag="VCI33_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP2832485.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
BASE COUNT         1466 a          676 c          878 g         1494 t
ORIGIN      
        1 tctataagag tctgatgtcg tgtaatcaat cactaaatga gtaggggtta gttgatagtg
       61 aatatcaggg caattttgtt ttaaaaagag agccaattga cgttcccgct gttttagata
      121 acgcttaata aagcgaagat ttttttgaaa catcccattt tctaaataaa gagctaatgc
      181 tttttgcatt agtaagttcg tgtctaaatc aatcagtgat ttttgggtta aaaaataagg
      241 ttttaaacct gacggtaaaa ctagagctcc gatacggagt gcaggaaaaa cactcattga
      301 aaaagatttt aggtagatga ttcggtgatg agtgtcataa taatgaattg gtggcttttt
      361 aagtttcaca aaatctccta aataatcatc ttctaaaata taaacctggt atcgttgtgc
      421 tagacgaaca atagcttcct tttccttagt gttataagaa agtcccagag gatgtgaaaa
      481 tcgtgaaatc gtgtaaaaaa atttaatatc gccagtttga aataaagatt ctaagagttc
      541 taaatcaagg ccattaaagt gtctagagat agtttgatag ggtaatccta actgagcaac
      601 aatagcttcc atccgatgat aagtaggctt ttcaaggaga attgttttgc ccgcattagg
      661 aaaaggcatt tgtgataaaa tataaagcgc ttgttgcgtc ccagaggtga ttaatagttg
      721 gtctttgtta ctgtaaacac tattttcagc taagtaaaga agcaaagctt cccttagttc
      781 ttctagtcct tcggttttgt gataataatg aaagaggtat ttgtctttag cacttaaagc
      841 ctcatttaag cagagtcgga aatcttcata agccatatta ttataatctt caagacttag
      901 attaagaaca ttatcgggca tgcttacgtt acccaaaaca taataaccac ttttaggaac
      961 agcataaatt aagtgacgat attttaattc taaaagagcc cgctgaacag tatctttgct
     1021 acaatgataa accttactta ataccctaat tgaaggtagc ttgtcccctt tttttagtcg
     1081 ctgtttctgg atatcttgtt cgatattgga aatgattgtt tgatattttg ttgtcataaa
     1141 ttgtccccat acagttaagt gttataacta ttgtatctta tgcctctttt tcttacaata
     1201 gttattatag aaaagaggta atagctatga aacggattat tgtggcaaat gatctggttg
     1261 gtgttggtaa ggtagctttg tctgctagta ttcctttaat ggcttcttgt ttgttagagc
     1321 aagttttatt accaacttgc cttttatcaa gccatactgg cggaggacag agccctgtga
     1381 agcatactac atcaagtata ttagacgttt ttttaacaga ttgggacaac aaaagaatta
     1441 agtttgacgg catctttata ggttatttta cgacaacaag agatgggcaa aagctactag
     1501 aatggctttc aagaaagcaa cttcccttaa ttttagatcc aattatggca gataatggaa
     1561 aactttatca aggcttcagt caaaaacata tagagattat gataaaactt actcaaaaag
     1621 ctgagcttgt tttacctaat attagtgagg cttgtctatt aacacaaaca gcttatttag
     1681 caaaaaatta tgatgaagaa gatattagac aggtggctag aaaattagct aagcttggac
     1741 ctaaacaggt tgtcattagc ggaatctctt ttgaggaggg cagcattggt tttgcttact
     1801 atcagtcaga ttcagatcaa ttaacattcc attttgggaa gcaattccct taccatttct
     1861 atggtacagg agatatagca tcagctatta taacagcagg attctattat cagctaccac
     1921 taacacaaat tctagatctt gttgtttcat ttcttgaaaa agtattgcaa aataccctta
     1981 cattaaaaag agatttaatc ttaggtgttg cttatgaatc tcatttaaat tacttaacta
     2041 aatcgtttaa aaagttgttg gaggaaaaaa atgtcatctc aaaaattagc taattatatt
     2101 gaattgagta tgtatgccgc tttaattttt atttcagtac aagtgttgcg tatccctatc
     2161 ggtatgcagt ttattcatct tggcaatgca ttagttgtta ttgcagtctt gatctatggt
     2221 agtaaaaaag gagctctagt tgctagtctt ggtttaggga tctttgatgt tttgaacggc
     2281 tatgcttcag tcgtttggat aactatctta gaatccttgg ctatttgttt aattttgcat
     2341 ttggtatatg aaagagctat gcaatctagt aaaaaaccaa aagccattat aacaataaca
     2401 gttattgctg cattgtcaaa attaactatt aaccttataa aatacttact gttagcttat
     2461 ataggtactc agctaccact ttcggtatct ttttgggtag ctcttggaaa aattggtggt
     2521 agtttcggaa catcttttgt gactttagtt gctgttcctc ttttgtatcc tgttatcaaa
     2581 aacttaaaag aaaaggttac aatcggacaa gcaaaaagag gtttttgata caagtacctt
     2641 atcgataaaa aagcaaggta cactttcagt caaatattga caaaaggtta caattttgcc
     2701 ataatgaaat aactataaag ccgttttgac ggcttttttg atgacagtta aggaagaaac
     2761 gatgagtatt ttagaagtta agcagttgag tcacggtttt ggggatcggg ctatttttga
     2821 aaatgtgtca tttcgcctct taaaaggtga acatattgga ctagttggag caaatggtga
     2881 aggaaaatca acctttatga gtatagtcac aggacattta cagcctgatg aaggaaaagt
     2941 agagtggtcg aagtatgtca ctgcaggtta cctggatcaa catacagtgt tggaatcagg
     3001 acaaaccgtt cgtgatgtct tgcgaactgc ttttgatgag ttatttaaga ccgagaatcg
     3061 tattaatgag atttacgcgt caatggcaga tgataaagct gatattgctg ttttgatgga
     3121 agaagtaggt gagcttcaag atcgtttaga aagtcgtgat ttctatactt tggatgctaa
     3181 gattgatgaa gtagcgcgtg cgcttggtgt tatggatttt ggaatggagt cagatgttac
     3241 atccttatca ggtgggcaac gaacaaaggt tttattagcc aaattactat tagaaaaacc
     3301 tgatattctg ctattagatg aaccaactaa ccatttggat gctgagcata ttgaatggtt
     3361 aaaacgctat ttacaacatt atgaaaatgc ttttgtgttg atttcgcatg atatttcttt
     3421 cttaaatgat gtgattaata ttgtttacca tgttgaaaat caaagtttag ttcgctatac
     3481 tggggattat taccaatttc aagctgttta tgagatgaaa caatctcaac ttgaagcagc
     3541 ctatgaacgt caacaaaaag agattgctaa cttgcaggat tttgtcaacc gaaataaggc
     3601 tcgggtagcg acacgtaaca tggcaatgtc tcgccaaaaa aaacttgata agatggatat
     3661 aattgaactt caagctgaga aaccaaaacc aaattttgaa tttaagcaag ctagaactcc
     3721 cagtcgattc atttttcaaa caaaaaatct tgtgattggt tatgattacc cattgaccaa
     3781 agaaccctta aatataacgt ttgaaagaaa ccaaaaaatt gctattgttg gggccaacgg
     3841 tattggaaaa tctactttgc taaaaagttt attaggtgtt atcgagcctt tagaaggtca
     3901 tattgtcaca ggggattttt tagaagttgg ctactttgaa caagaagtga caggtgttaa
     3961 ccgacaaact ccgctagaag tagtttggga tgcttttcct gccttaaatc aggcagaagt
     4021 tcgagcggca ctagctcgtt gcggactaac atcaaaacat attgaaagtc aaattcaagt
     4081 actttcgggt ggtgaacaag caaaagttcg tttttgtttg ttgatgaatc gtgaaaataa
     4141 cgtgcttatt ttagacgaac caacaaatca tcttgatatt gatgctaaaa atgagctcaa
     4201 acgtgcttta aaagcatata agggttctat tttaatggtt tgtcatgaac ctgatttcta
     4261 caatgggtgg gtaaccgata cttgggattt tagtaagtta acctaaacta ttccttaatt
     4321 ttttacacag gaattactaa aatcagtaaa gatatagaat aaatactgct agatatgtga
     4381 tggtttaggt aactgatcac caaccaaaaa agatgtgact acatcttttt tttatctaat
     4441 ggaatggtgt aaagtcgttt aataatagct tattttttga tgatcagttg ttcatgaaat
     4501 tgttggtgat taac
//
LOCUS       DATUYR010000089         4513 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.089, whole genome shotgun sequence.
ACCESSION   DATUYR010000089 DATUYR010000000
VERSION     DATUYR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.089"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(180..543)
                     /locus_tag="VCI33_001574"
                     /pseudo
     CDS             complement(180..543)
                     /locus_tag="VCI33_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(964..1497)
                     /locus_tag="VCI33_001575"
     CDS             complement(964..1497)
                     /locus_tag="VCI33_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEP2832486.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1577..2353)
                     /gene="recX"
                     /locus_tag="VCI33_001576"
     CDS             complement(1577..2353)
                     /gene="recX"
                     /locus_tag="VCI33_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEP2832487.1"
                     /translation="MKITKIEKKKRLYLIELDNDDSLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2468..3823
                     /gene="rlmD"
                     /locus_tag="VCI33_001577"
     CDS             2468..3823
                     /gene="rlmD"
                     /locus_tag="VCI33_001577"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP2832488.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITSVKRNFAEAKLLMVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT         1373 a          720 c          948 g         1472 t
ORIGIN      
        1 attgcagatt gaggtttaaa agacacttta caatgttctc aagtcatgtg ttgttgtagg
       61 ttgtatgcac atattacaaa tcccattata gaagaaattg tttaattaat ggatagtaat
      121 ggatggtatg gagatcttaa tgtggtttta atggtaattt tagcagttat agtaagacaa
      181 aaggtgactt ggaaaagctt atgcagacga ttcttaactc ccacaatatc ttctgtcaga
      241 ttttattaga cttcttctca gtgtcaattt tgtctgtttt acgaacgtgc aggctattca
      301 attgcttctt agcttctaaa ggattaaacc gagtataagc gtagccatta tctgcgaaaa
      361 aacctgaaga cgacgagaat agacattaat cgttttaagg ataatctcag ggttatggac
      421 ggttttcaag tttccaagaa gatgattgag gccgatggta tcgttttgtc ttgaatgatt
      481 ccttatttta gtggtttcat tttcaataca cgacgtcaag cgtcctacat actttgataa
      541 aattctacct aaaagaggtg tcttgtcagt tttggttaca acatcaagtg ccaaaagact
      601 ttgcgactta acaagacact tctactttaa cgcaaagaaa aagtagtgag tactctcttt
      661 cgtcggagat ttcctcgcta ctaaacttag tattttttga tactttcctt aggtagtgct
      721 taaggcagcg actttctttg gaattccatg ccttattttt gagatcctag gctcaaaaat
      781 gccaagtggc gtcaacatta acattgacgc cacttttacc acaacgaaaa acatctgttt
      841 tggctcactc cacacgcaga aataggaaat tggatgtcaa atcagcagag ctgttcatgc
      901 tttttgagcc taaaactgag tttgtctgcg ttctgagcac tccttgggag tgctggacaa
      961 ctcttaacga ttcttcagtt caagataacg tttgtaccaa atagtgatat aggccttgga
     1021 aaatggtcct ttttcatgat taatccaatc aaccaatatt ttgacatttt cttttaggat
     1081 aaagtccata tcggctgaat actgcatctc ttttttatga atctcgtatt catcaacatc
     1141 aagtaatctc ttttcaccat ccgcaaagac tttgacatct aaatcgtaat caatgtattt
     1201 gagggcctca gtgtccatca tgtatggact agctaagtta cagtaataag agacgccatt
     1261 atctcgaatc atggctataa tgttaaacca gtatttttta tgaaagtaaa cgattgcagg
     1321 ttcgcgggtc acccatcgtc taccgtcact ttctgtgaca agagtgtgat cattaactcc
     1381 aatgagggca ttttcagttg tttttagtac catagtatcg cgccaagtac ggtgcaaact
     1441 accatcatgc ttataacttt gaattgtaat aaagtcgcct tctttaggta atttcatgcc
     1501 ttaccaactt tctacaatta aacttatcct ctatagtcta tcataaatgt gtgaaaaaat
     1561 cacttctttt gtcagtctat aaataatttc ttaacttgca attaatatcg tcgctgttgt
     1621 agccttttcg gtagagagcc tgatagagct tttgctttaa agtataacca tcatatttgc
     1681 gactgagttt acgatattgt ttgtctaatt ctttgtcaag aagatcttct atttcttgat
     1741 tatcttggtc aaaattaagg tgattgaggc tatgtttagc tagatcgtat gaaaagcctt
     1801 tggtcagtaa tgcttgggtg attttatctt tcaaggcttt tggtggcaat ttttcttgat
     1861 atttgtcaaa gagtttttgg cttacttttt gagcgagttg gctaaagtca gtttgagata
     1921 agataggatc aatgtcatga cttgcaatgc ctttttgtaa taacttttgt tttaagactt
     1981 ggggaccttt atcaccattt aactgatttt ggcgaatgta ggtatcagcc aatttggtgt
     2041 cgtctatcca ttgttcttct tggagttgag tgatgatgtc agcaataatg tgttcttcaa
     2101 tctcatgctt gcgcaggtaa tcagcaactt gcttgttgct gcgttgttga aaggaaagaa
     2161 aataaagggc taaatttttg ccgtaggaca gttgggcaaa atgtttcatg tcttcaagct
     2221 gatcattgtc aaggacttta tctttactca acataaaccg aacaatagta tcttctgtta
     2281 cataaaggga atcgtcatta tccaattcga taaggtagag gcgttttttc ttttcaattt
     2341 tagtgatttt catatgttca ttatatcaaa aatggtaaaa tagaagacga aaatactgct
     2401 aaaaatgtgt tgcttgtgaa tgcttttggt ggtgagatta ggatattaga ggaagtaagg
     2461 ggaagagatg gtcgtaaagg ttaaacagaa aattccttta aaaattaagc gaatgggaat
     2521 taacggtgag gggattggtt tttatcagaa aactttggta tttgtccctg gtgctttaaa
     2581 gggagaagat attttttgcc aaatcacgtc ggttaaacgg aattttgcag aggccaaact
     2641 gttgatggta aacaaggctt ctaagaatcg tgtcaaacca gcttgttctg tttatgaaac
     2701 ttgtggtggt tgccaaatca tgcatctagc ctatcctaaa cagctagatt tcaaagatga
     2761 tgtgattagg caggccctta aaaagttcaa accaactggc tatgaacaat ttgagattag
     2821 accgacttta ggaatgaaaa agccagatca ctatcgagcc aaattgcagt ttcagctgcg
     2881 gtcatttggc gggacggtca aggctggttt attttcccag ggaagtcatc gcttggttcc
     2941 tattgacaat tgcttggtac aggatcaact aacgcaagat attatcaata agattacgca
     3001 gttagtggac aagtacaagt tgcccattta caatgagcgc aagattgctg ggattcggac
     3061 gataatggtt cgtaaggcac aggctagtga tcaggtacag attattgttg tgagtagcaa
     3121 agaagttcgg ttagcgaatt tcattggcga attgactaag gcatttcctc aggttaaaac
     3181 ggttgccttg aatagtaatc gttctaaatc gagtgagatt tatggggacg aaacggaaat
     3241 tctatgggga caagaggcga ttcatgaaga agttttagac tatggatttg cgttatcacc
     3301 gagagctttt taccaactta atccacagca gacagaagtt ttgtatggtg aggtggtgaa
     3361 ggccttggat gtagggagca aagatcatat tattgatgcc tattgtgggg ttggctcgat
     3421 tggctttgct tttgcaggga aggtcaagtc tgtgcggggg atggatatta tcccagaagc
     3481 gattgaagat gcacaaaaaa atgccaaagc catgggcttt gacaacgctt actatgaggc
     3541 agggaaggca gaggatatta ttcctaaatg gtataaacaa ggttaccgcg cggatgccat
     3601 cattgtggac ccaccaagaa ctggtttaga tgataaatta ttaaaaacga ttcttcatta
     3661 ccagcctaaa cagatggtgt atgtgtcttg taatacctcc actttggccc gcgacttggt
     3721 gcagctaaca aaggtttacg atgttcacta tatccaaagt gtagacatgt tcccccatac
     3781 agctaggacc gaggctgtcg tgaagttaca aaaacgtgta tgattgagtg gcaataacaa
     3841 ctcaagtttt ataagctcag gtaatgggtt tgagcaatag cttgacatag tagggatgtc
     3901 aaaaacggtt tactaagcat agtcatatta ttgataactc tatgtttatt ttagaaacat
     3961 tcatggttaa aaaagtttgt tagtttgtca ctacgtaggg tttatttgaa gagcaagtac
     4021 acacagacaa tattagggga attggttgtt gaaaaaaagc tgttttttgc tataaattag
     4081 aaaactgagt ggtcaggagg ttacattacc agaagttatt ttagtatgct aatatgaacg
     4141 atgatataga ggatttatcc tgatttaatc ttttaagctg taagaaacct ttgcaaaaac
     4201 tgtaggctgt gtgagtcttt atgtgttaga tattaaagat aaatgcaaca cttgtttata
     4261 atgagatcaa caactgataa atctgagttg ataaccagta aaatgatcgg aatatagaaa
     4321 agttacttaa tcaagctttt cgtaatttaa ctgttagaat gacagaactt atgataaaat
     4381 aaagaatatg attaaaatga aaaagctcag tttatctatt acggagttcg aaatgaaaag
     4441 aaaatcgaga tgtgataaca tattttgaac gagaaggaga aggataaatg ttagaagatt
     4501 ttcttcaatt ttt
//
LOCUS       DATUYR010000090         4445 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.090, whole genome shotgun sequence.
ACCESSION   DATUYR010000090 DATUYR010000000
VERSION     DATUYR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.090"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            6..851
                     /locus_tag="VCI33_001578"
     CDS             6..851
                     /locus_tag="VCI33_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEP2832489.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            complement(916..1476)
                     /locus_tag="VCI33_001579"
     CDS             complement(916..1476)
                     /locus_tag="VCI33_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEP2832490.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(1490..1960)
                     /locus_tag="VCI33_001580"
     CDS             complement(1490..1960)
                     /locus_tag="VCI33_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP2832491.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            complement(1950..2714)
                     /locus_tag="VCI33_001581"
     CDS             complement(1950..2714)
                     /locus_tag="VCI33_001581"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEP2832492.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            complement(2704..3453)
                     /gene="truA"
                     /locus_tag="VCI33_001582"
     CDS             complement(2704..3453)
                     /gene="truA"
                     /locus_tag="VCI33_001582"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEP2832493.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            complement(3638..4219)
                     /locus_tag="VCI33_001583"
     CDS             complement(3638..4219)
                     /locus_tag="VCI33_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP2832494.1"
                     /translation="MHNEKSNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLCRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGERFKGRRALLRKISPYFKEFAQQL"
     gene            complement(4215..4288)
                     /locus_tag="VCI33_001584"
     tRNA            complement(4215..4288)
                     /locus_tag="VCI33_001584"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4252..4254),aa:Arg,seq:acg)
     gene            complement(4368..4441)
                     /locus_tag="VCI33_001585"
     tRNA            complement(4368..4441)
                     /locus_tag="VCI33_001585"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4405..4407),aa:Asn,seq:gtt)
BASE COUNT         1404 a          987 c          655 g         1399 t
ORIGIN      
        1 ttctcatgac atttatatta cattacctag agcagtctca tatcgaaaat attggcttaa
       61 cgatttttaa aaagctagtg tctctcatta ttctattact cttttttgct attttaaaac
      121 gtgttactaa ctatcttttt gaaaaaacca ttaacaaatc gtttgcttat tcaagacaaa
      181 gtgaagctcg caaaaaaaca ctctctaagc tcactcacaa tattttaaat tacttattgt
      241 attttcttct catttattgg atacttagct tatttggcat acctgtctct agtcttctag
      301 caggagctgg aattgctgga gtagcaatcg ggttaggtgc acagggattt ttatctgacg
      361 ttgtcaatgg cttttttatc ttatttgaga atcaatttga agtaggagat aacgttacta
      421 tctctgacat agaaggcagt gtctttggtg ttggaattag aactactcaa attcgtggat
      481 tcgatggaac actacatttc atcccaaacc gtagcatcac cgttgtcagc aacaaatctc
      541 gtggtaatat gcgtgcctta attgaaattc ctctttattc cactgttaat ttgagccaag
      601 tcactcgaat cattgatgaa gtgaatcaaa aggaattgcc aaaccatcct caaatcgttg
      661 gcaaaccaaa tattttaggg cctcaaaata actctaatgg acagttcact tttagaattg
      721 ctattttcac agaaaatgga gaacaattta agatttacca tactttttat agactttatc
      781 aagaagcttt actcaaagaa ggcattcaat taccgactgc aatcagctat tctatcacct
      841 caaaatctta atataatctt aaaaaatcct tttttctaga aaaaacacca attgttttat
      901 tccagaaaaa agggtttagg atttacggat aatatccata ttgtaatcag ccctagctcc
      961 accaattaat tttggtcgac tagccaaagc tgtcacatga gcacctccta gctctcgttg
     1021 acaaggaatc agagggatct gaacacgctt aatatgcatt cctatagcag tatcacctat
     1081 atcaagtcca gcgtgtgcaa taacttcttc tacttcgaca gggtctgaca taaattgaaa
     1141 agcagccatc tgagcactgc caccagcatg aagattcgga accacattga caatctctaa
     1201 ctgcttagat tctgccacat gcctctctac taccaaggca cgattgacgt gttcacaacc
     1261 ttgtacagcc aaatgaacac cacgcttatt taattcatct aacacaactt caacaactat
     1321 ttgacctact tctagactag attgttttcc aattctacta cccaaaattt cgctagatga
     1381 aagacctaga acaaaaagat tacctggttg aatagcagag cgttctacaa catcaatgac
     1441 gatttccctt gtttgtttct ctaaattatt caacaacttc tcactctcct tatctctttt
     1501 tttgccttaa tatggcctta aagataataa aaccaacaaa aagccctaat gtattttgca
     1561 tgacgtttcc ccaaataccg gctaaagatc ccgaaaggcc ataaccaagc ataagagagc
     1621 ctaaaaagta ccaaaaaatc atgataaaag aacctatcac aacaccaagc caacgcttgc
     1681 gccctctcca tcccgcaaaa tacccttgga cactgtgagc aatcaaacta tgaaacatcc
     1741 actgagggta tcctgccact aaatcgatta aaaaacctga taaccctccc acaatagctc
     1801 cttgagctga tccaaaggaa aagctgacag cataaattcc tgcatccaac agcgttaaaa
     1861 aacctgttgg agtaggtagc atgacaaatc ttcctaaaac aacaaccaat gctgtcaata
     1921 tccctgttaa actcatttgt cgtatcttac tattttgcat aactttgttt tactccgtat
     1981 ctatcagatt gtaaaatagc ttggtagact aactcttttg agtttttcac agcttctagt
     2041 ggcgtttttt tctttactaa ctgactagcg atactagatg caaaagtaca gcctgcgcca
     2101 atattatttt tctcaagtac aggacattcc aaggtaacaa tctctttacc gtcataaaaa
     2161 agatcaatag cctttttctg actaaatcga ttaccaccct tgataaccac ctgttttgcc
     2221 ccaagttgat aaaaatattt agcagcttct tgcatatctt ttaaagaaac aatctctttt
     2281 tgagataata gctgagcctc taccaaattt ggagtaacaa cagttacata aggcaataat
     2341 tgtaaaatct cctgtcttag aggaactatt ttaacatcat ctatttcttt acatgctaga
     2401 acaggatcta aaaccactgg gattcccaga tgccccttta taaaatccaa aactatttca
     2461 cacatttccg cattaggtaa caaaccaatt ttaatagcag aaattggagc gttcgtaaag
     2521 ctattcaact gatcacggaa aatttcttta gctaccggaa atagactaaa gccctcttca
     2581 gatctagtag ttaaacaagt taccgctaca aaggcctgta ggtcatacct tatgtaagtc
     2641 gctaaatcag catagagacc acctccactt aaaatatcgt tcccagatat agtcacgata
     2701 taatcagttt tcataacaaa tctccttcaa gtaaagccca tttccagata tcgttggtcc
     2761 agctagctga cgatttttgg agctcaaaat cactttaact tgttcaactg gcatttgacc
     2821 attaccaatt ttcagcaaag ttcccaccat attcctcacc tgcttataga gaaaaccatt
     2881 tcctgaaaaa gtaaacacca aaaaatcagt tttttcatcc cgagataccg ttgctttggt
     2941 aatcgttcgc accttatttt gtaccgaagt tcctgctgct gtaaaaccag taaaatcgtg
     3001 tgtacctacc agaccgttaa tagcttcttg cataagttta atattaagag tataagggta
     3061 atgagtggta tagtgtcgca tcatcggatt tttaggtcga ccattatcaa ctaaaaattc
     3121 ataagttttt aaatgcttct gataacgaca atgaaaatca tcagccactt tttctatatt
     3181 aacaacatca atatcttcag gagtctgtgt atcaagagca aatcgtaatt tttcaacttc
     3241 ttgttcttga ggcaaatcaa aatggatcac ctgtccataa gcatgtactc ctgcatccgt
     3301 acgtcctgca ccatgaatta taatttttgt accgttattt aatttataga gtgttttttc
     3361 aatctcttct tgaaccgtac gtaaatgtct ttgtctttga aaacccgaaa aaagagtccc
     3421 atcataggaa atcgttgctt tgtatcttac catgttacta ttttaacatt tcttaaacgt
     3481 aagagaaact atttttaatc aaactgtacc caaacaaatg aaagacgtat tgaagcttac
     3541 tctaagccac tagcgattta aaagctataa gaaagctatt tcctccaaac tctctccttt
     3601 tttcgtagat ttacccctaa ttccttatag gtcttgctta caactgctgt gcaaattcct
     3661 taaaataggg actgatcttc ctaagtagag cacgtcttcc cttgaaacgt tcgcccctga
     3721 taagtgcttg aaactgcata cgctcttgcg aatttaattg gttctctaag ctagctatta
     3781 cctcttgata agccacatag tcgtctagcc ataaccctct cgatggaatg gcatgtgcaa
     3841 cctcccctat ctcttcatat gctaacttat ggaactgacg cttttgactt tcttgacggc
     3901 ataataaatc tttcaaataa gatgaaaatt ttgttttaaa atagcgatat aaacgctctt
     3961 cttcctcaat taactctggg tacctttcta gtaactgtaa taagataata tgtccttctt
     4021 gtaaccagtc atctctatcc cacaattgaa tataatagtg tcgctttaat ttcaaaataa
     4081 tgggcttaac tttttcaaac gctgctcttg tctctatcga cattttgagt ctccttttct
     4141 ttttaactat ccctagtata aagagaacca caaaaaaaca acaggacact aatgtcctgt
     4201 tgcttttttc attatgcacc ctagaggagt cgaacctcta accgcctgat tcgtagtcag
     4261 gtactctatc cagttgagct aagggtgcca acgcatatct tgcctctatc ttaccacact
     4321 cttctctaaa atgcaaaaaa agataggacg cttcctacct ctctctactc cgccagtagg
     4381 actcgaacct acgacatcat gattaacagt catgcgctac taccaactga gctatggcgg
     4441 ataaa
//
LOCUS       DATUYR010000091         4419 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.091, whole genome shotgun sequence.
ACCESSION   DATUYR010000091 DATUYR010000000
VERSION     DATUYR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.091"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            18..2291
                     /locus_tag="VCI33_001586"
     CDS             18..2291
                     /locus_tag="VCI33_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEP2832495.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEQLSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     GLQTLSQGNSLAQLDQLKAAVSQIASQSNQALPGASSALSELSMGLSKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTVIGSGVIKLSEGANALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(2408..3481)
                     /locus_tag="VCI33_001587"
     CDS             complement(2408..3481)
                     /locus_tag="VCI33_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEP2832496.1"
                     /translation="MKNISRKCFMTSVVCIVLGAILLGAGYATGGLQDIKHQTAPKKV
                     IQTFDHVTALDIDTNFSTITVETGPVKKPTVTYYTHSKFIDPLEIKIENETLHISEKP
                     RDVIITGGIELIGFGLNKSDRNHNYRTTTITLPKGTSLKELKGSSMPQATISDLTVQD
                     LEFSGGLTLLNTRIKKGKVFGTFNATDSLLANLELKADYSLSSLTNSSLENGTISLGH
                     GQLTTKNTNLKSVTIQSLSPGGVETEETSLENVTFTVTSEQENEETNDNGAIFTAHAL
                     TLKGTNTISGGNVAVDITLTDPKTTAYKTKTEDGKILLGSQLTPAKIGKESTSDVISY
                     VAENKAATGNLTVNLNKGDITIK"
     gene            complement(3478..4065)
                     /locus_tag="VCI33_001588"
     CDS             complement(3478..4065)
                     /locus_tag="VCI33_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEP2832497.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEALEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATALLRFSGRAFVAFIKWISHKKGAQA"
     gene            complement(4052..4378)
                     /locus_tag="VCI33_001589"
     CDS             complement(4052..4378)
                     /locus_tag="VCI33_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP2832498.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
BASE COUNT         1316 a          857 c          891 g         1355 t
ORIGIN      
        1 ccattttagg agaaaatatg ttagaagaat tgaaaacact tattaaaaat ccaaaattaa
       61 tgattacaat gattggtgtg gccctagtgc ctgccttata taatttatcc tttctaggct
      121 caatgtggga tccttatggt cgggtcaatg accttcccat tgctgttgtt aatcatgata
      181 agcctgcaaa gagagctgat aagtcattga caattgggaa tgatatggtg gacaagatgt
      241 ctaaaagtaa agatttagag tatcattttg tctcagctaa gcaagctcaa gagggactta
      301 aagaaggtga ttattatatg gttattactt tacccgaaga tctttctcaa cgggcagcaa
      361 ccttattaaa tcccgaaccc caaaaattaa ctatccgtta ccaaacgagt aaaggacatg
      421 gaatggtcgc tgctaagatg ggggaaacag cgatggctaa gctgaaagag tctgtttcgc
      481 aaaacattac gaagacttat acctcagcag tttttagcag tatgacagac ctccaatcag
      541 gattaaaaga agcctcagct ggaagtcaag cacttgcttc aggagcgaag accgctcagg
      601 cggggagtca aacactttcg acgaacttag cagccttaac gggtgctagc caacagtttc
      661 aacaaggtac tggtcgattg acatcaggtt tgactaccta tacagatggt gtcaaccaag
      721 tcaagaatgg gttaggaaca ttatcaacgg acatccccaa ttatctgaac ggggtttcta
      781 ggttatccca gggagcttct cagcttaatc agggtctttc acagttgaca caagcaacaa
      841 cactttctga cgagaaagct aagggaattc aatccttaat tgtaggacta ccagtcctaa
      901 atcaaggcat tcagcaacta aatacagagc tatcaacatt gcaaccccct aaccttaatg
      961 ctgatgagtt aggtaatagc ttaggagcta tcgctcaagc tgccaaacaa gttattgctg
     1021 aagagactgc cgctcagaat gaacaactat cggctctcca agccactagt gtttatcaat
     1081 cattaactgc tgaacaacaa ggagagttag ctgcggccct cagtcaatct gataaaagtc
     1141 aaaccgtatc tgcagcccaa actattttaa gttctgttca aacactgtca acaggtttac
     1201 aaactctgtc ccaaggaaat agtttagctc aattagacca gcttaaagca gctgtttcac
     1261 agattgcgag tcagtctaac caggctttac ctggagcgag ttctgcatta tctgagttgt
     1321 caatggggtt atcaaaggta aatggtagct taaatcaaca agttctacca ggaagtaatc
     1381 aattgacaac aggattagca caattaaaca ggtataatac tgtcattggt tctggggtaa
     1441 taaaactctc agaaggtgcc aatgccttgt catccaagtc cggagaatta ctagatggta
     1501 gccatcaatt atcagagggt gctactaaac tagctgatag tagttctcaa ttgagtcagg
     1561 gtggtcatca attaacgagc ggattgactg aattatcaac aggattatca accttaaatg
     1621 gttccttagc caaagcctct cagcagttat cgcttgtttc tgtgactgat aaaaatgcta
     1681 aagctgtcgc aaaacctctt gtgttaaatg agaaagacaa agatggtgtt aagacgaatg
     1741 ggatcgggat ggcaccttat atgattgctg tttctctaat ggttgtggcc ctttcaacca
     1801 acgtcatttt tgctaattct ttatctggtc gtccggtcaa agataaatgg gattgggcta
     1861 aacaaaaatt tgttattaat ggttttattt cgactatggg atccattgtt ctctacttag
     1921 ctattcaatt attagggttt gaagcccgtt atggtatgga aaccttagga tttattatgc
     1981 taagtggttg gacgtttatg gctcttgtca cagctttggt cggttgggat gatcgatatg
     2041 gctcttttgc ttctttggtc atgttattac ttcaggttgg ctcttcaggt ggctcttacc
     2101 ccattgagtt aagtggagca ttcttccaaa agttacatcc tttcttacca atgacttatg
     2161 tggtatctgg tttacgacaa accatttcat tatcaggtca tattggagta gaagtgaaag
     2221 tcttaactgg tttcttactg gcatttatgg tattagcact actcatttat cgtcccaaga
     2281 aaacagtcta aataaaataa cctcaaaaaa aaacagcatc taaggcagtt acactagatg
     2341 ctgttttttg tcttattttg acaataactt gacaatttct gtcaagtttt ttattgtctt
     2401 gttaagatta tttgatagta atgtctccct tattgagatt aaccgttaaa tttccagtag
     2461 ctgctttatt ctcagccaca taagaaataa catctgaagt tgattcctta ccaatcttag
     2521 ctggtgtcag ctgtgatcca agtaagattt taccatcttc ggtttttgtc ttataggcgg
     2581 tcgtctttgg atcggtcaaa gttatgtcaa cagcaacatt accaccacta atagtattag
     2641 tgcctttaag ggtaagagca tgagcagtga atatagcacc attatcattg gtttcctcgt
     2701 tttcttgctc actagtaaca gtgaaagtca cattttcaag gcttgtttct tcagtttcaa
     2761 ccccaccagg agatagagat tggatggtga cagatttaag attagtattt tttgtggtta
     2821 attgtccatg ccctaagcta atcgtcccat tttctaaact agaattcgta aggcttgaca
     2881 acgaatagtc ggctttcaac tcaagattgg ctaacaagct atcagtagcg ttaaatgttc
     2941 caaaaacctt ccctttcttt attctagtat ttagaagtgt cagaccacca ctgaactcaa
     3001 ggtcttgaac cgttaaatcc gatatagtgg cttgaggcat gcttgagcct ttgagctctt
     3061 taagactggt accttttgga agcgttatgg tagtggttcg gtagttgtgg ttgcggtcac
     3121 ttttatttaa tccaaacccg atcagttcaa tacctccggt gatgatgaca tctctgggtt
     3181 tttcagaaat atgaagtgtc tcgttctcta ttttaatttc taaaggatca ataaatttag
     3241 aatgtgtgta ataggtaacg gttggttttt tgacaggccc tgtttctact gtaatagttg
     3301 aaaaattggt atcaatatca agagctgtta cgtgatcaaa tgtttggata acctttttag
     3361 gggctgtttg gtgtttaatg tcttgaagcc cgccagttgc ataaccagct cctaaaagaa
     3421 tggctcctaa aacaatacac acgacactgg tcataaaaca tttgcgtgag atatttttca
     3481 tgcttgagct cctttcttgt gactaatcca tttaataaag gcaacgaagg cacgtcctga
     3541 aaaacgcagg agtgcagtgg caataatcca aatgagaaga ccaccaccaa aacacaacaa
     3601 ggcgctgccg attcccatag cttgtgcagg aatagatgag ccaatcaagg tcaagctttc
     3661 aaacagggtc actcctgcga caagaatacc tgcaagccca aaaatgtagg cggcaaagat
     3721 agctgtgagc ccgagcatta ggcaggcaat aataaagaga atcaacgcca gcaatacggg
     3781 taaagcaatc ggagcagcaa agagggacaa aatggttaat ccaataattg ttgcccgatt
     3841 tttgggagtt ttctcgggag ttgccaagtg ttttcctaga acatttgaaa tgatttcact
     3901 ggcagcttcc ttaggatttc ctaaatcgtc gataacctgg ctttctttgt caggaccagc
     3961 ttcatcaaaa tattcaatat aatattccaa cgcttcatgg tagtcttctc taggtagttt
     4021 gcgcaagtat ttatctagtt ctgctagata ctcagtcctt gtcatgtctt aagctccctt
     4081 caataatccc gtctaaggca aatttatagc tctgccattc tttttttaga aaaatcagct
     4141 gtgctctccc cgaagaagtg acagcataat atttacgctt tcgtccctga tgttcttgac
     4201 tataagttgt caaaaaacct gccttctcca attttttaag aataggatag agggtggatt
     4261 ctttgatatt agccactagt ttaatggttt gactaatatc gtaaccgtaa gaatcgtttt
     4321 tttcgacaat ggctaaaatc agaaattcaa tcaaggtagc cgatactgga aaatacattt
     4381 taggctcctt tcatatataa aaattttata tataattat
//
LOCUS       DATUYR010000092         4311 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.092, whole genome shotgun sequence.
ACCESSION   DATUYR010000092 DATUYR010000000
VERSION     DATUYR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.092"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(167..1771)
                     /locus_tag="VCI33_001590"
     CDS             complement(167..1771)
                     /locus_tag="VCI33_001590"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEP2832499.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRNVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            complement(2028..2603)
                     /locus_tag="VCI33_001591"
     CDS             complement(2028..2603)
                     /locus_tag="VCI33_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEP2832500.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            complement(2815..4098)
                     /locus_tag="VCI33_001592"
     CDS             complement(2815..4098)
                     /locus_tag="VCI33_001592"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEP2832501.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
BASE COUNT         1261 a          940 c          660 g         1450 t
ORIGIN      
        1 ccaagtattt tacaaaaaaa tgactgaaaa agaaagtgct aacctaaaaa tatcacgttt
       61 ttctttttct aatgctattt ttatagaaaa gatccctcta atagctagta atctttatca
      121 gtatctgcca gccaagtaga atgccacaaa cttaccctta aaaccactaa ttgctatttt
      181 taatagcagc tgtaacaaaa gcggtgtata gctcctcagg acgatttgga cggctttgca
      241 attctgggtg gtattgagct gctacaaaga attttttctc tttcaattct acaatttcaa
      301 ctaagcggtt gtcaggtgat acccctgaga aaacaaaacc agctgcttca aattctggac
      361 ggaatttgtt gttgaactca taacggtgac gatgacgacg ttgcaccacc tcttgattat
      421 tgtaagccat tgctgctttt gaacctggtt tcaatttaca tgggtacaat cccagacgaa
      481 gcgtacctcc catatcttca atatcaattt gatcacgcat gatatcaatg atggggtatt
      541 tagtacttgg ctctaattca aaagagtttg caccttccat attgaggaca tggcgagcaa
      601 attcaacaca agtcagttgc atgccaagac agataccaag cattggcaca tcattctcgc
      661 gggcatagcg aatcgcttgg atcttgcctt ctgtaccacg ttgaccaaaa ccacctggta
      721 caataatccc atccgcatct cccaacaagt cagctgcatt atccaccgtc acatcgttag
      781 cattgaccca ttttaggtca attgcggtat cattggcata accagaatgt ttcaaggctt
      841 ccacaacaga taagtaggca tctggcaatt ccacatattt accaaccaag gcaatttttg
      901 ttgtttttct taaattcatc accttgtcaa ccatagctga ccactcagtc atatcagctt
      961 gtggcgcatt caatttcaaa tgatcacaaa caatctgatc cattgattgg gcttgcaagt
     1021 tcaatggaat ttgatacaag tgttctacat tacgtgactc gataacagct tctgaattca
     1081 catcacagaa ctgagccaat ttatttttaa tcccctgctc aactggctct tccgtacgaa
     1141 taaccaacat attcggttga atccctaaac cacgcaattc tttaacggaa tgttgcgttg
     1201 gcttagtttt catttcaccc gctgccttta aatatggcaa caaggtggtg tgaatataca
     1261 tcacattttc agaaccaaca tcagctttca tctgacgaag agcttctaag aacggtaaac
     1321 tttcaatatc gccaacagtc cccccgacct cagtaataat tacatcagag tccgttgttg
     1381 aagctgcgcg tttgattttt tctttaaggg catctgtgat atgaggaatc acctgaacag
     1441 tcgctccaag gtactcacct ttacgttctt tacgaaggac ttcactataa attttacctg
     1501 ttgtaacatt ggaatattta ttaagattga tatcaataaa acgttcatag tgacccaaat
     1561 ccaagtcggt ttcagcaccg tcatctgtca catatacttc tccatgctga taggggctca
     1621 tcgtccctgg atcaatatta atataaggat caaatttttg aatcgtcacc ttaagtccac
     1681 gattttttaa caatcggcca agacttgccg caacaattcc tttgccaatt gatgatacaa
     1741 caccaccagt tacaaaaatg tatttcgtca tagtactcct ttatataagc tgaataaaag
     1801 cattgctttc ttgttaattc cctattagtt ggttccacat tggttaggta tcaccttagt
     1861 tttctaaata tagaggcaaa ctggcattat tctagaaaac aaaaatagcc ccctattaac
     1921 tagagagctt ttgcctgacc tcataaagag gtgcccgaat aataatataa actataatag
     1981 acaatttgtc aaatgataat cacattttta tttctgaact aaagacatta gagaactggt
     2041 tcttcatctt cttcatcttc ttcgtcttct tcattgatat caacttcttc aaaatcatct
     2101 tcagggataa tttcattcaa ttctgaatca tatgactcca cttctgattt ttcatcatct
     2161 ggatcttctt catcatattc aacctcagct gattcttctg tgaaatcttc atcttctgga
     2221 tcatcatctc tataatcaat agcgtcttca tctccatcca tgaaagcatt aacgcgtttt
     2281 ttcttacgtt tttgtgcgcc atcttcatct tcttctagag taataatttc ttcatcaatt
     2341 tcatcaatag cataccacga acgaagtccc catttgtttt cacctagagg aataaatgag
     2401 ccatccgtat ttaagtcagt atagaaaaat ggtaatgcat gacgaatacc tgcatcagat
     2461 tttcctaagt aattttgaat ctcattgact aaatcgctaa agtacatctc attgtcgcga
     2521 ccgcgttctt ccaaaatggc acgcgccact tcaatcatgg aaagctcgct tttttcttgt
     2581 cctgcaaata cgtctaattt caaggcagtt ctccttattt tatcattctc taccatttta
     2641 cgctaaaaaa agtaattcgt caaaactttt atcaactcat atcacttctt cttgacaaat
     2701 ctttaaagct aattcattaa aataacgttt aactaattcc ttatctttag aaaaagaaaa
     2761 accagactag aaagacgttc taatccggct tgtacgtttt gttccttaat aagattactt
     2821 aacgcttgct gtgcttgtaa tcacttcaac tgcttttttc attgcaatat catgtttcaa
     2881 catatctgct gaaagaagag aacgaacttg gtcagctggc atgttatatt ctgttgcaag
     2941 gtcattaatt tcttgttcaa tttcactatc tgttgcttca aaaccttctg ctttagcaat
     3001 tgcttcaata acaaggtttg ttttaacacg cttgtcagct tcagctgaat attggttatg
     3061 taaatcttct tgagttgtac cagtcaattg gaagtacatt tcaggtgaga ttccttgacg
     3121 ttgcatgttg cccataaatt cattcactga acggttgact tcttcatgaa tcatttcttc
     3181 gggtaaatca acaatttcag catttgcaac tgctaattca atcgcagctc cttcaacagc
     3241 atcatcataa gcagtttctt gagctgcttc aagttcttta cgatatttta cttttaagtc
     3301 ttcaagtgtg tcaacatctt catcaatatc ttttgcaagc tcatcatcaa gctctggtac
     3361 ttcttttgtt ttgacttcgt gaatagttgt cataaattta gcggctttac ctgcaagatc
     3421 ttctgcttgg taagattctg ggaatgtgac attaacttct acttcatcgc cagctttagc
     3481 accaactagt tgatcttcaa aacctgggat aaattgtcct gaaccaagtt caagagagaa
     3541 gttatctcct ttaccgccat caaactcaac accatcaact gaaccaacaa agtcaatcac
     3601 aacagtgtca ccttgagctg cttcaccgtc tttaataatg agttccgcaa ggttttgacg
     3661 ttcgcgctca attttagcat ccacgtcttc atctgaaact tcttttgaag catcaacttc
     3721 tacaacaagg tttttgtaat caccaagttt cacttcaggt tttgtcacaa cttcagcaga
     3781 aagtgtccac tctttccctt tttccattga cacaacatcg atttttggtt gtgcaaccac
     3841 atcaagacca agttccgtca cagctgcttc ataagcttct ggcaatacaa tattcaaagc
     3901 atcttcataa agaacttctt caccaaattt ttggttgaag actggacgag gcatgtgtcc
     3961 tttacggaaa cctggtgcat ttaagtcttt tttgatttta ttaaaagctt tatcaagagc
     4021 tggttttatt ttatcttgac taattgtaaa tgtaatcacg ccacgatttg tagctttgtt
     4081 ttcaaatgat gtagacatta agtcatttct ccttaaaata atttcgatac agttcatttt
     4141 atcataattt aaagcgtttt taaagctttt tatgcgtgaa atcaaacgcg ctattaagag
     4201 tatctacctg tcattagtct actaacagtt attttcaatt tataaaggtt aatgttagac
     4261 tttttcgctt actaactgac tattttcaag cattatcaca acggatgact a
//
LOCUS       DATUYR010000093         4083 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.093, whole genome shotgun sequence.
ACCESSION   DATUYR010000093 DATUYR010000000
VERSION     DATUYR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.093"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            198..1691
                     /gene="malQ"
                     /locus_tag="VCI33_001593"
     CDS             198..1691
                     /gene="malQ"
                     /locus_tag="VCI33_001593"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEP2832502.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            1726..3990
                     /gene="glgP"
                     /locus_tag="VCI33_001594"
     CDS             1726..3990
                     /gene="glgP"
                     /locus_tag="VCI33_001594"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEP2832503.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNTA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNFWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKGIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
BASE COUNT         1340 a          725 c          794 g         1224 t
ORIGIN      
        1 caatgaacag aatcttagtc agactatttt tgttcctttt agtttaaaac aaagagaaag
       61 cgtccgtgat attagtcatt aagagcaaaa aaaagtagca tttttaagga atgttacttt
      121 ttttaggaaa ctggttgact tacgcaagcg cttgcgttat aatctcatca tgaatagatt
      181 tcacaaggag aaaaatcatg aacaaacgtg caagcggtat cttaatgcat attagttccc
      241 tgcctgggaa atttgggata ggcacttttg gaaaatcagc ttttgaattt gttgattttc
      301 tagcagagac gaaacaaacc tattggcaaa ttctgccttt aacaacgacg agttttggag
      361 attctcctta tcagtcattt tcagctattg ctgggaacac acatttcatt gattttgaat
      421 tgctagtaga tgatgaactt ttagaagcag cagatttgtg tgatattaca ttcggcacaa
      481 atcctgaagc agtagactat gctcagcttt ttcaagttag gcgtccactt ttagaaaaag
      541 cagtgagagc ttttgttgct gaacaagaaa atgtgtgtaa attagaagct tttgaaacag
      601 cttctagctg gttaactgat tttgctgaat ttatggcttt aaaagaatat tttaacaata
      661 aagccttaca agattgggac gacgaaactg tcattaaacg ccaagaagac tcacttaata
      721 attaccgtga gttgcttgca aaaaagatta cctatcataa agtctgtcaa tacttctttt
      781 accaacaatg gtcagccttg aaaacttacg ctaaccataa agggatagaa attattggtg
      841 atatgcctat ttatgtttct gcggacagtg tagaagtttg gacgatgcct gagctattta
      901 aagtagatag tgataaaaag ccattgttta ttgcaggtgt accagcagat ggctttagtg
      961 aagatggtca gctgtgggga aatccaacct acaattggtc tgctcatgaa aagtctaatt
     1021 ttgcatggtg gatttatcgt attcaagaaa gtttcaaact atatgatcag ctaagaatcg
     1081 atcattttaa aggcttctca gatttttggg aaattcctgc tggtgataaa actgctagaa
     1141 atggccattg ggcctcagcc ccaggtatag cattattttc tgcggttcgt gaggccttag
     1201 gagagctccc tatcatcgca gaaaaccttg gttatattga tgaaaaagca gagcagctat
     1261 tagcatcaac aggctttcct ggcatgaaga tattagaatt tggtttattt gatataacga
     1321 gtcaaagtat agatttacca cattactatg accgtaattg tgtcgcttac acagggactc
     1381 atgataacga ggtggttaac ggatggtatg acaatcttag tgaagaacaa gtacactttg
     1441 taaataacta tcttcacaaa catgcagatg aaagtattac aaaagccatg ctaagaacaa
     1501 tctttgcttc tgtttgtgat acagccattc tttgcatcca agatttatta gataaagatg
     1561 gtaagagtcg tatgaatatg ccaaatacaa ttggaggtaa ttggcaatgg cgtatgcttg
     1621 acggtgaatt gaaccaagat cacaaagatt accttattta tttaacagat ctttatggtc
     1681 gcgcaagata atatttaaat gttacattat tgaaaagagg aagacatgac acgctttaca
     1741 gaatatgttg aaactaaact aggaaaatca cttacgcagg ctagcaatga agagatttat
     1801 ctatcattat taaactttgt caaagaagaa gctagccaca aagctaaaaa tactgccaaa
     1861 cgcaaagttt actatatctc agcggaattt ttgattggta aattactatc aaacaacctg
     1921 attaacctag gaatttataa agatatcaaa gaagaattgg cagctgctgg caaatctatc
     1981 gcagaagtcg aagatgttga attagaacca tcactaggta atggtggttt aggacgcctt
     2041 gcttcatgtt tcattgactc tatttcatct cttgggatta atggtgaagg ggttggttta
     2101 aactatcatt gtgggttgtt taagcaagtc ttcaaacaca atgagcaaga agctgagcca
     2161 aacttctgga ttgaagatga ctcatggttg gttccaacag acatttctta cgatgtgcct
     2221 tttaagaact tcaccttgaa atctcgtctt gatcgtattg atgttttggg ttacaaacgc
     2281 gacactaaaa actaccttaa cttgtttgat atcgagggcg ttgattacgg gttaatcaaa
     2341 gacggtattt catttgataa aacgcaaatt gctaaaaact tgaccttgtt cctctaccca
     2401 gacgattctg acaaaaatgg ggaattgctc cgtatttacc aacagtactt tatggtgtca
     2461 aatgcagcgc aattaatcat tgatgaagct atcgaacgtg gttcaaacct tcatgacctt
     2521 gcagactacg cttacgtgca aatcaatgac acgcatccat caatggtcat cccagaatta
     2581 attcgtctct tgactgaaaa acatggcttt gactttgatg aagcggtagc tgttgtgaaa
     2641 aatatggttg gttacactaa ccacactatt cttgcagaag cccttgaaaa atggccaact
     2701 gcttacttaa acgaagtagt gccacacttg gtaaccatca ttgaaaaatt ggatgctctt
     2761 gttcgttcag aagtgtctga tccagctgtt caaattattg acgaatcagg tcgtgtgcac
     2821 atggcccata tggatattca ttttgcaaca agtgtcaatg gggtagcagc actccacaca
     2881 gaaatcttga aaaacagcga attaaaagct ttctatgacc tttacccaga aaaattcaac
     2941 aacaaaacca acgggattac tttccgtcgt tggctagaat ttgctaacca agacttggct
     3001 gattacatta aagaacttat tggcgatgag tacttgactg acgcaacaaa attagaaaaa
     3061 ttgatggcct ttgcagatga caaagctgtt catgctaaat tggctgaaat caaattcaac
     3121 aacaaattag ctcttaaacg ttaccttaaa gacaataaag gcattgagct tgatgaacat
     3181 tctattattg atacccaaat caaacgtttc cacgagtaca aacgtcaaca gatgaatgct
     3241 ctttacgtga ttcacaaata tttggaaatt aaaaaaggca accttccaaa acgtaaaatc
     3301 actgttatct ttggaggtaa agcagcgcct gcttacatca ttgctcaaga catcattcac
     3361 ttgatccttt gcttgtctga attgattaac aatgaccctg aagtaagccc ataccttaat
     3421 gtgcatctag ttgaaaatta caacgtgaca gtagcagagc acttgattcc agcaactgat
     3481 atttctgagc aaatttcact agcatccaaa gaagcttctg gaactggtaa tatgaaattc
     3541 atgcttaacg gtgctttaac acttggtaca atggacggtg ctaacgtaga aatcgctgag
     3601 cttgcaggca tggagaatat ctacaccttt ggtaaagatt ctgacaccat catcaacctt
     3661 tatgcgactg cttcttatgt agcaaaagat tactatgata accatcctgc tattaaagca
     3721 gcagtgaact ttattatcag tccagaattg ctagcatttg gcaacgaaga acgtcttgat
     3781 cgtctttata aagaattgat ttcaaaagac tggttcatga ctttgattga ccttgaagag
     3841 tacattgaag tgaaagaaaa aatgttagca gactatgaag accaagattt atggatgaca
     3901 aaagtggtcc ataacatcgc aaaagctgga ttcttctcat ctgaccgtac cattgagcag
     3961 tataacgaag atatttggca ttcacgataa aaaataataa ttgcaatgtg gggtagcggt
     4021 accgtaacat gcttaaacta aatcataaga cagtagtttc tgctgtctta tgatttttct
     4081 tca
//
LOCUS       DATUYR010000094         4077 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.094, whole genome shotgun sequence.
ACCESSION   DATUYR010000094 DATUYR010000000
VERSION     DATUYR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.094"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            110..340
                     /locus_tag="VCI33_001595"
     CDS             110..340
                     /locus_tag="VCI33_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEP2832504.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            342..2024
                     /locus_tag="VCI33_001596"
     CDS             342..2024
                     /locus_tag="VCI33_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP2832505.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PNGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(2252..3598)
                     /gene="glnA"
                     /locus_tag="VCI33_001597"
     CDS             complement(2252..3598)
                     /gene="glnA"
                     /locus_tag="VCI33_001597"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEP2832506.1"
                     /translation="MAITVADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPIEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFISQWEIDHYIHNY"
     gene            complement(3636..4007)
                     /locus_tag="VCI33_001598"
     CDS             complement(3636..4007)
                     /locus_tag="VCI33_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP2832507.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
BASE COUNT         1259 a          812 c          727 g         1279 t
ORIGIN      
        1 tactacacaa taatgacaac atataaaacc gtcaaaatgg aacacctacc ttttgaccta
       61 tttattattg tttggataat tgattcataa cgaaaggaat aaaaaacaaa tgatttataa
      121 agttttctat caagaaacaa aggatcaaag cccacgtcgt gaaagtacta aagcactcta
      181 tcttaatatt gatgctactg atgaactcga tggccgtatc aaggctcgtc gtctcgttga
      241 agataacacc tattataacg tggaatttat cgagctactt tctgacaaac acctcgatta
      301 cgaaaaagag accggtgttt ttgaattaac ggagttctaa gatgacaaat atcagtttaa
      361 aacctaatga agttggggtg tttgccattg gtggtctggg agagattggg aagaatactt
      421 atggtattga gtatcaagac gagatcatta tcgttgatgc cggtatcaaa ttcccagaag
      481 atgatctttt gggaattgat tacgttattc cagactattc ttacattgtc gataacttag
      541 accgggtaaa agctcttgtt attacacatg gtcacgaaga ccacattggt ggtattccat
      601 ttcttctcaa acaggctaat atccctatct acgcaggacc tttagcactt gctcttatcc
      661 gtggtaaatt ggaagaacat ggtctttggc gtgaagcaac tgtttacgaa atcaatcaca
      721 acaccgagtt aacttttaaa aacatgagcg tcactttctt caagactact cattccattc
      781 cagaaccggt cggtattgtt atccacaccc ctcaaggtaa gattatctgt actggtgatt
      841 ttaaatttga cttcacgcca gtaggagatc ctgcagattt acaacgtatg gcagcgcttg
      901 gtgaagaagg tgtcctttgc ttattatcag actcaactaa tgctgaaatc ccaaccttta
      961 ctaactctga aaaagttgtt ggccaatcca ttttaaaaat tatcgaaggt attcatggtc
     1021 gtattatttt tgcttccttc gcttcaaaca tctaccgttt gcaacaggct gcagaagctg
     1081 ctgttaaaac gggacgtaaa attgccgttt ttggtcgttc tatggaaaaa gcaattgtta
     1141 acggtattga acttggctat attaaagttc caaatggtac cttcattgag ccaagcgaac
     1201 ttaaaaatct tcatgctagt gaagttttaa ttatgtgtac aggtagtcaa ggtgagtcta
     1261 tggcagcact tgctcgtatc gctaatggca ctcatcgtca agtaacttta cagcctggtg
     1321 atactgtgat tttctcatca agtccaatcc ctggtaatac cacaagtgtc aataaattaa
     1381 tcaataccat ccaagaagct ggtgttgatg ttattcatgg aaaagtcaat aatattcata
     1441 cttctggtca tggaggacag caagaacaaa agctcatgct tagtttgatt aaaccaaaat
     1501 atttcatgcc agtacatggt gagtatcgca tgcaaaaagt tcatgcagga ctggcgatgg
     1561 atattggtat tccaaaagaa aatatcttta ttatggaaaa tggagacgtt ctagctttaa
     1621 ccagtgacag tgcgcgtatt gctggtcact ttaatgccca agacatctac gtggatggta
     1681 atggcatcgg ggatattgga gcagccgttt tacgagaccg ccgtgatttg tcagaagatg
     1741 gtgttgtctt agctgttgca actgttgatt ttaacacaca aatgatttta gctggaccag
     1801 atattctgag ccgtggtttc atttatatgc gtgagtcagg tgacttgatc cgcgaaagcc
     1861 aacgtgtgct ctttaacgct atccgtattg ctttaaaaaa taaggatgct agcatccaat
     1921 cagttaacgg tgctatcgtc aatgctcttc gtccattctt gtatgaaaag actgagcgtg
     1981 aaccaattat aattccgatg gtcttaacac ctgataagca ctaaaatcaa ataagactca
     2041 tttcttaata ctatctgtct atatctttaa catatcagtt ctagttcaga taattatcaa
     2101 actaaaaacc agtcctcata gaacatggac tggtttttag ttttctataa tggaaaatca
     2161 attggtgtcc gatcagctaa taatagcttg ctagctcaaa tacaatagct catccaaaag
     2221 acccactcaa cttgagcagg tcctgataag tctaataatt atgaatataa tggtcaattt
     2281 cccattgaga aataaaagtt gcataggaag accattcaat tcgttttgct tctaagaaat
     2341 tagtgtagat atggtaacct agtgcctttt gtaccacatc atctttttga agagctttta
     2401 aggcattatg aagcgttgat ggcaaatcaa taatgcctgc ttcatttcgt tcttccattg
     2461 tcatggtata aatgttagct tcaatgggtt ctggagcttc aattttgtta atgataccat
     2521 ctaatccagc ttccaagaga acagccaagg ctaaataagg attagctgtc ggatcaaccg
     2581 aacgtaactc caaacgcgtt cccataccac gtgatgctgg aacacggata agcggtgaac
     2641 gattacttcc agcccaagcg acataaacag gtgcctcata acccggaact aatcgtttgt
     2701 aagaattcac tgtagggtta gtgatagcag tgtagttata agcatgcttc attagtcctc
     2761 ccaagaaata ataagcatct tctgatagct gcattcctcg cttatcagtt tcatcataaa
     2821 aagcattatt accttggtta tcaaacaaag acatgttaca gtgcatccct gatccagcta
     2881 ttccaaattt tggtttagcc ataaaagtag cataaagtcc atgttcacgg gcaatcgttt
     2941 ttacaactag cttaaaaatt tgaatattat cacaagcttt caaaacatct gcatatttaa
     3001 aatcaatctc atgttgacca acagccactt catgatgact agcttccact tcaaaaccca
     3061 ttttcgttaa aatattcaca atttcacggc gcgtgttgtc tgctaagtca attggcgcta
     3121 aatcaaaata accaccatta tcgttaactt caagtgtcgg attaccttta tcatccatct
     3181 taaaaaggaa aaattctggt tctggcccaa gattaaatga tttgtagccg atctcgttca
     3241 tgtgtttcag ggctcttttt aaatttcctc taggatctcc tgcaaaaggc tttccttctg
     3301 ctgtataaat atcacaaatt aaacctgcaa ctgctccatt ttcatctccc cagggaaaaa
     3361 caatccaagt gtctaaatcg gggtaaaggt acatatctga ctcattgatc cgtacaaaac
     3421 cttcgataga tgaaccatca aacataacct tgttagacaa tactttgtct aactgttctt
     3481 tcgttgcagg aatctccaca tttttcataa cgcccatgat atcagtgaac atcaagcgaa
     3541 gaaacgttac atttttttct ttgacttcat gacgaatgtc agctactgtg attgccatta
     3601 aataggtctc ctttaaattt tatacaattc atatcttaaa tacgaaaatt accaatatgc
     3661 tgtgagggag tagaaaaacc gctttgtgtc agcatttcat catgtaagat ccgtctgaca
     3721 tctgcatctg ttagagcttt ttgtttttgc attaacttac cttgacgctc gacatattcg
     3781 cgtttaatag ctgcaatatt taatccctca gacaaaaaat ctttaatttc caaaagtcgg
     3841 tccatgtcat ttaaggaaaa catacgccga ttcccttgcg ttcgttcagg cttaattaaa
     3901 ccttgatctt cataatagcg aatttgtctc gcagagagat ctgttaatgt catcaccgtt
     3961 ccaataggaa aaacagccat tgagcgtcta agttcttttt ctttcatgac aaactccttt
     4021 catcttcctt attataggct gaaaaaaata acctgtcaag tatatatgtt atattat
//
LOCUS       DATUYR010000095         4038 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.095, whole genome shotgun sequence.
ACCESSION   DATUYR010000095 DATUYR010000000
VERSION     DATUYR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.095"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            102..1100
                     /gene="ruvB"
                     /locus_tag="VCI33_001599"
     CDS             102..1100
                     /gene="ruvB"
                     /locus_tag="VCI33_001599"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEP2832508.1"
                     /translation="MARILDNDVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            1238..1675
                     /locus_tag="VCI33_001600"
     CDS             1238..1675
                     /locus_tag="VCI33_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEP2832509.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMSKEY"
     gene            1698..2099
                     /locus_tag="VCI33_001601"
     CDS             1698..2099
                     /locus_tag="VCI33_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832510.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            2096..3871
                     /locus_tag="VCI33_001602"
     CDS             2096..3871
                     /locus_tag="VCI33_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEP2832511.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLLWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNYSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMIGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGSLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
BASE COUNT         1238 a          629 c          835 g         1336 t
ORIGIN      
        1 actatctttt aaagtagttc aagagcttag tgctgttgac tggtggcgat tataataaaa
       61 gtaggccact gctctctttt actttatgtt ataatagtcc tatggctaga attttagaca
      121 atgatgtaat gggaaatgag gagttttcgg atcggactct tcgccctcaa tatctacacg
      181 aatatattgg acaagataaa gttaaggaac aattcgctat ttttatagaa gcagctaaaa
      241 gacgtgacga atctttagac catgttttat tatttggacc tccaggactg ggaaaaacaa
      301 caatggcatt tgtgattgcc aatgaattgg gagttaattt aaagcaaact tcaggtcctg
      361 ccgttgagaa agctggagat ttggtagcta ttttaaatga attagaacca ggggatattt
      421 tgtttattga tgaaattcac cgcatgccaa tgtctgttga ggaagtacta tatagtgcta
      481 tggaagactt ctatattgat attatgattg gcgctggaga tactagtcgt agtattcatt
      541 tggatctgcc tccatttaca ttgattggtg ctactacacg tgcaggcatg ttatctaatc
      601 ccctcagagc tcgttttggt atcactggtc acatggaata ctatcaagaa aaggatttga
      661 cagagattgt ggagcgtaca gctactattt ttgaaataaa aatagaccat gaagctgcac
      721 ggaaattagc ttgtcgcagt agaggaacgc cccgcattgc taatcgatta ctaaaacgag
      781 tgcgtgacta tgctcaaatt attggtgatg gcatcataac ggctcaaata actgatagag
      841 ctttgactat gttagatgtt gaccgcgaag gtctcgatta cattgatcag aaaattttac
      901 gaacgatgat tgagatgtat cagggaggac cagttggttt ggggacatta tctgtgaata
      961 ttgccgagga gcgtaataca gttgaagaga tgtatgaacc ctacttaatt caaaaaggct
     1021 ttctcatgcg aacacgaaca ggtcgtgtag cgactcaaaa ggcttatcgt catcttggct
     1081 atccctatca aaacacttaa tgatttttca aatctaagaa aattaatctg aacctgcttt
     1141 ttgtgttcgg attttttgat aagttgacag aaaattatta gattgatttc tccgatatca
     1201 gttagaaatt tgatataata attcaaagga gtatttaatg aaaaaagtat gttttgtctg
     1261 tctgggtaat atttgtcgaa gccccatggc tgagtttgta atgaagtcaa tagtatcaag
     1321 tgatgttatg atgatagaaa gtcgtgctac ttctgattgg gagcatggaa accctattca
     1381 ctcaggtaca cagtccatat taaaaactta ccaaattaat tatgatataa ctaaatgctc
     1441 caagcagata actataactg attttaacac ctttgactat attattggta tggatagcga
     1501 taatgtgaaa aatctcaaag agatgtcgca acatcaatgg gactctaaaa tctacctgtt
     1561 tagagaaggg ggtgttcctg atccttggta cactaacgat tttgaagaaa cctaccaatt
     1621 agttagaaaa ggatgccagg attggctttc tcgtttaatg agtaaagagt attgatatta
     1681 gaagttgagt aaaagaaatg atagatatgc aagatatagt aaaaaaatgg agtattacac
     1741 gggcgaagtt ggaaattgtc tcggtgattg tcatattggt ttgtgcaatt tctgttttta
     1801 gcgtaagaat ttcgaataag acaagtttga cctatgataa agggcgtatg cactatactg
     1861 gttacgtgat caaccataaa atgaatggag aagggaagct tgtctacccc aacggtgata
     1921 tctatgaggg tacttttaag aatggcctat ttgaaggaaa aggaactttt actgctaaga
     1981 cgggctggtt atataacggg gagtttcata aaggacaagc aaatggcaaa ggtgtgttaa
     2041 aagcaaagaa taataaagtc tataaaggga tttttaaaca ggggatattt caaaaatgag
     2101 aataaaatgg ttttcgtttg ttagggtaac aggactttta ctggttttac tctaccactt
     2161 ttttaaaaat gtatttccag gaggttttat tggtgttgat attttcttca ccttttcagg
     2221 atatctaatc acagccctct tgattgatga atacacaaaa aaagagtcta tcgatattat
     2281 cggtttttta aaaagacgct tttatcgaat tgttcctcct ttggtgttaa tgattttatt
     2341 gaccattcct tttacgtttt taattaagaa ggatttcatt gctaatatag gaagtcagat
     2401 tacagctgtt ttaggattta ccactaatat ttatgaaata ttgacgggaa gtagttatga
     2461 aagccaattt attccacatc tctttgttca tacgtggagc ttagctattg aagttcattt
     2521 ttacttgttg tggggtgtat ttgtttggtt attagctaga cgcaaggaga ctcagaaaca
     2581 actgagagga ctactgtttt tgatttcttt gggaatattt gctattagtt tcttgagtat
     2641 gtttataaga tcttttatga cctcaaatta ttccctgatt tatttttcga gtctttctca
     2701 cagttttccc ttctttttag gggcgatgtt tgccacaata acaggtatta acgagactac
     2761 tgttcgattt caaaaaaatg tacgtttatg gccgcgccag tatgtccttg ctgctatgat
     2821 tggagcattt acacttttgc ttgtgctaac ggtcacattg gattttaacc atatcacgac
     2881 ctatctattt ggctttgctt tggctagctt attcgcatca attatgattt atgcagcgcg
     2941 tgttcttcat gagcaaacac cagatgtaca agagcctaaa gctatcactt atatagctga
     3001 tattagctat gggatttacc tcttccattg gccattttac attattttta gccaattaat
     3061 gtctcatatt ttggcagtta ttctaacagt tttcttttca atcttgtttg ccactgtgtc
     3121 ttactatatt gtggaacctt tggtccaagg gagaaaacca aacctgcttg gtttagaaat
     3181 tgactgttct ccttactata agtggattgt aggagggtct ctcgccttag cattattaac
     3241 tttaggaact tgtatgatag cgcctaaagt tggtaagttt gagaaacaat tgttggtcag
     3301 ttccctacaa caggctcaaa gtaatatgga gcggacacat acactagctg caggagacgc
     3361 taatgctttg agtgacgtag gtattattgg tgattctgta gcattacgct ctagtgcagc
     3421 attctcaaaa attatgccac aagcgcaact ggatgcagcg gtcagtcgga attttaaaga
     3481 agctttcgat ttatttaata accaaattaa gtctaagagt ctatctaaga ctgttgtctt
     3541 agcagtaggt gttaactcgc ttgacaacta ttctcaagca gttcagtctt ttattgaggc
     3601 attgccaaaa ggacatcgtt tagttttggt ttctccatat aatgccaaaa atgccagtca
     3661 ggttgcagaa gctagagact atggtcttaa attgtccaaa aaatacaagt atgtcactat
     3721 tgcagactgg tataaagttg ctgttgaaca tccagatatt tggtatggca gtgacggtgt
     3781 tcattatagt gaagatagtc aaggagcaga actatatgtc tccacaatcc agactgctgt
     3841 tgagaagtca gctaaaaaac cagctaaata aagttaagaa atgtctaaga gataagtcag
     3901 atccattgtg ctctggcttt ttcttgtgaa caaatgatga tggtaaaata gtagtacttt
     3961 gaggttttta aaagaaagcg atttcagaaa taaaacggct ttttttagat atatttctga
     4021 aaaagagtta atgaataa
//
LOCUS       DATUYR010000096         3944 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.096, whole genome shotgun sequence.
ACCESSION   DATUYR010000096 DATUYR010000000
VERSION     DATUYR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.096"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     misc_feature    12..147
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            176..676
                     /locus_tag="VCI33_001603"
     CDS             176..676
                     /locus_tag="VCI33_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEP2832512.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            741..1106
                     /gene="rplL"
                     /locus_tag="VCI33_001604"
     CDS             741..1106
                     /gene="rplL"
                     /locus_tag="VCI33_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEP2832513.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
     gene            1365..1964
                     /locus_tag="VCI33_001605"
                     /pseudo
     CDS             1365..1964
                     /locus_tag="VCI33_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            2139..2464
                     /locus_tag="VCI33_001606"
                     /pseudo
     CDS             2139..2464
                     /locus_tag="VCI33_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            2455..2729
                     /locus_tag="VCI33_001607"
                     /pseudo
     CDS             2455..2729
                     /locus_tag="VCI33_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2778..2987
                     /locus_tag="VCI33_001608"
     CDS             2778..2987
                     /locus_tag="VCI33_001608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832514.1"
                     /translation="MTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLEFLLN
                     RANSLEELLLKAKEVNLTIDLKQKM"
     gene            3252..3944
                     /locus_tag="VCI33_001609"
                     /pseudo
     CDS             3252..3944
                     /locus_tag="VCI33_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
BASE COUNT         1356 a          624 c          844 g         1120 t
ORIGIN      
        1 taatgatata ggttttgata aaaaactgac ctaagacagc aggggagcat gctcataata
       61 ttcctgccga ggcacaaaac gtgattaaag aaaataacgt atttgtactt tcgtgtctag
      121 gtttaggtgc gatttttttg tacctagccc aaaaataaaa acggaggtaa aactaatgag
      181 tgaagcaatt attgctaaaa aagctgaaca agtcgaactt attgctgaga aaatgaaagc
      241 agcagcaagt atcgttgttg ttgattcacg tggtcttaca gttgatcaag atactgttct
      301 tcgtcgttca cttcgtgaaa gtggcgttga gttcaaagtt atcaaaaact caattttgac
      361 tcgtgcagct gaaaaagcag gtcttgatga gttgaaagat gtattcgtag gaccatctgc
      421 agtagcattt tctaacgaag atgttatcgc accagctaaa gttatcaacg actttactaa
      481 aactgctgac gcacttgaaa tcaaaggtgg tgcaatcgaa ggcgctgttt cttcaaaaga
      541 agaaatccaa gcacttgcaa cattgccaaa ccgcgaagga atgctttcta tgcttctttc
      601 tgtacttcaa gctccagtcc gcaacgttgc atacgctgtc aaagctgttg cagaaaacaa
      661 agaaggcgct gcttaagacg tctcagagta gcttaaggct acgcttaaca ttttaaatta
      721 attatttgga ggaaatcaca atggcattga acattgaaaa cattattgct gaaattaaag
      781 aagcttcaat ccttgagctt aacgatcttg taaaagctat cgaagaagaa tttggtgtaa
      841 ctgcagctgc tcctgtagct gctgcagctg ctggtggtgc tgaagaagct gctaaagatt
      901 cattcgacgt tgaattgaca tctgctggcg acaaaaaagt tggcgttatc aaagctgttc
      961 gtgaaatcac aggtcttggt cttaaagaag ctaaaggtct tgttgatgga gcacctgcta
     1021 acgttaaaga aggcgttgct gctgcagaag ctgaagaaat caaagctaaa cttgaagaag
     1081 ctggagcaac aatcactctt aaataagaag cagatagcaa tttgagagcg aaatcccgat
     1141 ttatcagtct tttagagctt atagcgattt gaagaaactg ataaaatgat ttggtttttt
     1201 gccaaaaatc ctgccaaaaa aatctttggc aggattttta ttttctataa aaattaagct
     1261 gagtagagtg gttattctat ctatatgtgt ctagtaataa tttttttatt ttcaggagga
     1321 aaatcatgca aatgaatgaa cagtcatcac gacaaatggt gattgccatc tatctcattt
     1381 attccaactc aaaatgatag tactccttgg ctgttccaag tcgaaattgt tagtgattaa
     1441 gaaaacctac gagactatgg tttgatcgat gaaggccagc agtctcccag tgaaaaaggg
     1501 cgtttggcta ataagattta tttaggaaag ttggaacatg atttcacccc agtcttaagt
     1561 tcagacgggg ccagtctcaa aaacagacca gccccgtgtt aaattcagcc cctagtgaga
     1621 ctgaaaagag tgaaactgaa ggtagtgaaa ctaaagagag taatttagtc attgaggacg
     1681 aggaggaaag aaaggagtgc actagtgtga aaaagactga gggtcacttt actcgtcaag
     1741 ttgatcatgt caccaaatat gacaaagatt atatttggag tttggttcat tcccaattac
     1801 gtgaaggtgg tttatcacaa gctgctagtg acttagtcat gagctatttt gaagaacgct
     1861 atgcttatgc gctggaacac atacggttcg caagaacagc tgaagctatt gcggaatatg
     1921 tctttaatgg tgtcttatcc gaatggacca agcaactaag gtgacaagaa gcgaagtgag
     1981 taagatgttt tggtggctat tattaggtgt ttggggacta aggacaattt ggttgattat
     2041 tgaagttatc actgcaccag aaatggaaga ttatagttga gtaaggacgt tacataagca
     2101 gtaaggtcct tttattttgt cagaaaggaa tgagagatat gcagttttta gatttgtttg
     2161 caggaattgg aggctttcga ttgggtttaa ttaatcagtg ccatgagtgt attggctttt
     2221 gtgagattga taagtttgcg aggcaatctt acaaagccat ctatgagacc gaaggagaaa
     2281 tagaatttca tgacattaga caagtcacag accaagactt tagacaactt agagggcaag
     2341 tggatatcat ctgcggtggt ttttcccttg tcaagcattt tcactcgcag gcagacgact
     2401 gggatttgag ggcactagga gaactctctt ctttgagatt actcgagcga ccaaacagat
     2461 aaaaatcata cccataatca catccaatca acgccattga ccgtaattca gataaaaaat
     2521 tgatatggaa ttatgccttg gaacgaaacc tccgtatgat tttagaccgt atttcaaaaa
     2581 tgactggagc aaaaattatc gagaagcgtt attcctaccg tgactatcaa aaatataggc
     2641 agtctagtca taaatttgaa ttgaaacaac gtctttattt tttgatgcag cagtcaaagt
     2701 tctttgatga ttttttagaa aaagcagagt aattacatgt tcatattgat tttagtcaga
     2761 agcatagcag attcatgatg acggatcgag taatgaaaaa accaattcga ggccgtcaac
     2821 tcagtaaacg agatttatat gatgaagaat tttttcgtac acattttgcc aagatagaaa
     2881 ttgaaagtcg attagaattt ttgttgaacc gtgccaattc attagaggag ctactattaa
     2941 aagcaaaaga agtgaatcta accattgact taaaacaaaa aatgtaactt ttatcctaga
     3001 agaagatact caaaagataa gtttaggtca taaaaaaata agcgataaga aattatatga
     3061 tgtcaatttt ttcaagatta ctttaaaaat aaggaagtcg gtgtttcaga tcgattagaa
     3121 agtctacaga aacagtacat gcttttcaag aagaacgaga taaagaggag gtatccactg
     3181 aagagagtaa ggaagtcttc aagaaataca aggggcaacg agatgccatc catgagtttg
     3241 aagttgaact tgtggaacat caaattgaga ggttaattgc tgaggacatt tatattaagg
     3301 tgtcttttgg tgtaaagcag agtggtctta ttttcattcc taactatcaa ttggatatta
     3361 ggaaagaaga aaatcataaa aaatataaag tctatattcg tgagaccgca caattcttca
     3421 tttacaacaa ggaggcgtct aaacttaatc gctatatgcg agggcgtgag ctcatttacc
     3481 agttaaccaa tgatagcaag agtattccaa aaagaagacg accaaaacca ttgatacact
     3541 taagaaaaag attgaagaaa taagtctcct cattgagtta gatacggaga ataagcctta
     3601 tcaagatatt aaagatgaca ttgttaaaga tatggctcaa ttagatttga ccattacaga
     3661 gttgcaggac catattgctc atctcaataa ggttgcggag gtcttgctta atatgaacaa
     3721 caacgatata gagaaccgcc gtttggccag atatgactat gccaagatga acttgactgc
     3781 agctataaaa atagaagaag ttgagaaaga gattgaaact tctcaaaatg aacttaatat
     3841 atccatagat gagtatgaat atctagtaag aaggttggaa aagtttggag agatcttgag
     3901 ttatagcaaa attatcgata cttctcgaaa tgaaatacaa tggg
//
LOCUS       DATUYR010000097         3819 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.097, whole genome shotgun sequence.
ACCESSION   DATUYR010000097 DATUYR010000000
VERSION     DATUYR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.097"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            17..1240
                     /locus_tag="VCI33_001610"
     CDS             17..1240
                     /locus_tag="VCI33_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEP2832515.1"
                     /translation="MPSMKYILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            1299..2105
                     /locus_tag="VCI33_001611"
     CDS             1299..2105
                     /locus_tag="VCI33_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEP2832516.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLL"
     gene            2338..3693
                     /gene="dnaA"
                     /locus_tag="VCI33_001612"
     CDS             2338..3693
                     /gene="dnaA"
                     /locus_tag="VCI33_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEP2832517.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
BASE COUNT         1356 a          658 c          641 g         1164 t
ORIGIN      
        1 attcggagga caaaacatgc caagtatgaa atatatctta aaatccttaa gtattttact
       61 aattggattt ttaggaggat taatagcaat tattacattc aataatctct acccacattc
      121 tccttcaaaa ataaattcag gtaaggcaac aactagtaat atggttttta acaacacaac
      181 caatactact aaagctgtca aagctgttca aaatgcagtt gtatcagtta ttaattatca
      241 agataaccct tcttcatccc tttctaaccc ttatacaaaa ctctttggag aagggcgttc
      301 aaaagagaat aaggatgctg aattatctat ttttagtgaa ggatctgggg tcatttatcg
      361 aaaagatggc aactccgctt acgttgttac taataaccat gttatcgacg gagctaaacg
      421 aattgaaatt cttatggcag acggatctaa agttgttggt gaattagttg gagctgatac
      481 ttattcggat ttagctgttg ttaagatctc ttcagataag ataaaaacag tagctgaatt
      541 tgcagactct acaaaactaa atgttggaga agttgctatt gctatcggca gcccactagg
      601 aacacaatac gctaattctg ttactcaagg aatcgtctct agtttaagtc gtactgtaac
      661 tttaaaaaac gagaatggtg agactgtctc aacaaatgct attcagacag atgctgctat
      721 taaccctgga aactctggtg gaccactaat taatattgag gggcaagtaa tcggaattaa
      781 ttcaagtaaa atttcttcta ccccaactgg tagcaacggt aatagtggtg ctgttgaagg
      841 aattggtttt gctatcccat ctactgacgt tattaaaatt attaaacaac tagaaactaa
      901 tggtgaagtt atcagacctg ctcttggaat atccatggtc aatttaaatg acttatccac
      961 aaatgctctt agccaaatta atattccaac tagtgtaact ggtggtattg tagtagcaga
     1021 agttaaggaa ggaatgccgg catctggcaa acttgctcag tacgatgtga ttacagaaat
     1081 tgatggcaaa acagttaatt caattagtga tttacaaagt agtctatacg gacacgatat
     1141 taatgatact attaaagtaa ctttttatag aggaactaca aagaaaaaag cagatatcaa
     1201 attaacaaaa actactcaag atttgactaa aacgcagtaa acaagctacc taaatactat
     1261 tacattttat cacataaaac agtccaaagg actgttttat gtttcttatt ctatccagga
     1321 actcccttat gacaaaagaa ttattgatag acctaccaat tgaagatatt gttacaaatc
     1381 cctatcaacc aaggatacaa tttaatcaaa gagaactcca agacctagca acttctatta
     1441 aatcaaacgg cctcattcaa cctattattg ttcgaaaatc tgacattttt ggctatgagt
     1501 tagttgctgg agaacgacga cttaaagctt caaaaatggc tggtctaaaa aaagttccag
     1561 ctatcatcaa gaagatctct acactcgaga gtatgcaaca agctatagtt gaaaatttac
     1621 aacgttctaa ccttaacgct atcgaagaag ctaaagccta tcagttattg gttgaaaaaa
     1681 aacacatgac tcacgatgag attgctaaat atatgggaaa atcaagacct tatattagca
     1741 ataccttacg tctgttacaa ctcccagcac ctatcattaa agcaattgaa gaaggaaaaa
     1801 ttagtgcggg acacgcgcgt gctcttttaa ctttgagtga tgataagcaa caactgtacc
     1861 tcactcataa aatacaaaat gaaggcctaa gtgttaggca aattgagcaa ctggttactt
     1921 ctactccaag ttcgaagcta tctaaaaaaa ctaaaaatat ttttgccact tctttagaga
     1981 aacaattggc taaatcattg ggactctctg tcaatatgaa gctgacagca aaccatagtg
     2041 ggtaccttca gatatctttt tccaatgatg atgaattaaa cagaattatc aacaagctac
     2101 tttagcttgt tgatattctg ttttttcttt tttagttttc cacatgaaaa atagttgaaa
     2161 acaatagcgg tgtcacatta aaatggcttt tccacaggtt gtggagaacc caaattaaca
     2221 gtgttaattt attttccaca ggttgtggaa aaactaacta ttatccatcg ttctgtggaa
     2281 aactagaata gtttatggta gaatagtgct agaattatcc acaagaagga acctagtatg
     2341 actgaaaatg aacaaatttt ttggaacagg gtcttggaat tagctcagag tcaattaaaa
     2401 caggcaactt atgaattttt tgttcatgat gcccgtctat taaaggtcga taagcatatt
     2461 gcaactattt acttagatca aatgaaagaa ctcttttggg aaaaaaatct taaagatgtt
     2521 attcttactg ctggttttga agtttataac gctcaaattt ctgttgacta tgttttcgaa
     2581 gaagacctaa tgattgagca aaatcagacc aaaatcaatc aaaaacctaa gcagcaagcc
     2641 ttaaattctt tgcctactgt tacttcagat ttaaactcga aatatagttt tgaaaacttt
     2701 attcaaggag atgaaaatcg ttgggctgtt gctgcttcaa tagcagtagc taatactcct
     2761 ggaactacct ataatccttt gtttatttgg ggtggccctg ggcttgggaa aacccattta
     2821 ttaaatgcta ttggtaattc tgtactatta gaaaatccaa atgctcgaat taaatatatc
     2881 acagctgaaa actttattaa tgagtttgtt atccatattc gccttgatac catggatgaa
     2941 ttgaaagaaa aatttcgtaa tttagattta ctccttattg atgatatcca atctttagct
     3001 aaaaaaacgc tctctggaac acaagaagag ttctttaata cttttaatgc acttcataat
     3061 aataacaaac aaattgtcct aacaagtgac cgtacaccag atcatctcaa tgatttagaa
     3121 gatcgattag ttactcgttt taaatgggga ttaacagtca atatcacacc tcctgatttt
     3181 gaaacacgag tggctatttt gacaaataaa attcaagaat ataactttat ttttcctcaa
     3241 gataccattg agtatttggc tggtcaattt gattctaatg tcagagattt agaaggtgcc
     3301 ttaaaagata ttagtctggt tgctaatttc aaacaaattg acacgattac tgttgacatt
     3361 gctgccgaag ctattcgcgc cagaaagcaa gatggaccta aaatgacagt tattcccatc
     3421 gaagaaattc aagcgcaagt tggaaaattt tacggtgtta ccgtcaaaga aattaaagct
     3481 actaaacgaa cacaaaatat tgttttagca agacaagtag ctatgttttt agcacgtgaa
     3541 atgacagata acagtcttcc taaaattgga aaagaatttg gtggcagaga ccattcaaca
     3601 gtactccatg cctataataa aatcaaaaac atgatcagcc aggacgaaag ccttaggatc
     3661 gaaattgaaa ccataaaaaa caaaattaaa taacatgtgg aaaagaatat cttttatgaa
     3721 atagttatcc acaagttgtg aacaaccatt tagtcttgga ttctctcgtt tatttagagt
     3781 tatccactat atacacaaga cctactacta ctacttatt
//
LOCUS       DATUYR010000098         3714 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.098, whole genome shotgun sequence.
ACCESSION   DATUYR010000098 DATUYR010000000
VERSION     DATUYR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.098"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            22..1530
                     /locus_tag="VCI33_001613"
     CDS             22..1530
                     /locus_tag="VCI33_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP2832518.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HRQQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIGNFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
     gene            complement(1879..3228)
                     /locus_tag="VCI33_001614"
     CDS             complement(1879..3228)
                     /locus_tag="VCI33_001614"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEP2832519.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKLEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
BASE COUNT         1114 a          727 c          657 g         1216 t
ORIGIN      
        1 tttaaggaga gaaaaattac attgttagat tattatttgg aatccgatat tattgaccag
       61 aaaatcctac tggctatttt gttgacagaa aaagaactga cgatggcagc agcttgttct
      121 caaacaactt tgactgcttt aaaaataaag cagtatcttc gacaatttaa cgccttattt
      181 aaagggtact tacgtattga gcttattaaa tctcgtattt attgtgaggt attaaatgat
      241 aagcgagaag cattttttta tgatattttt gccttatcag atactctaaa aatgttgaca
      301 ttcttattac tagataatcc caaacacaaa tcaatcgctg tttatacacg aaaacaaggg
      361 atatcccagt caaaagctta tcgtcttatt cataaattaa aacattattt acaagacatt
      421 ggtttaaata ttgtagacaa tactgttatc ggagatgaac ttaaaatacg gtatttgatt
      481 gctcttttac ataaagagta cggcattata ttatatgata tacaacctgc agacattgaa
      541 acgattcatg cttttatctt tgcagcacaa aaaaacttac aaccttctgc ctttttagat
      601 agacgcttct tgttttttga tgtgctgttg atgttgactt ggaagcgcca tcgctatcct
      661 gttcatttgc ctcatttagc tctttttgaa catctcaaat ccttgcctat ttttgacaat
      721 atcaaaacca ttgccgtgga tgagctagca cctcgaactc gtgtcacatt ttcgtctgat
      781 gattttgact atctcttttt aatttattta acgactgata actccttctt gagtgggtac
      841 tggacaagtc atcggcaaca acaactttac catttaatca ccaaagatcc cgattatcat
      901 ttactaatcc atcgtcttca ggcactagtt ggaaaatatt ccgatattca tgagcatatc
      961 cctaacttaa ttcctttttt caaaagaact ctctacaatt tacaaacttt aattaccttt
     1021 gatggttatt attttgatca atatcaaggc aacatgctac tactagataa gcttgaaacc
     1081 gttatcaaag attggcttta tgacacaggc cgtcaaggca gtattagttc tggtcactta
     1141 cacttgatgt gtttatacct tgagcagatc ttggaaagct ctattgctcc aatcaatatt
     1201 actgttattg agagccaaga aactgttggt aacgttatag gtaatttcat cacctcaact
     1261 atcccttctt acaaagttga actgtcacgg gtcaatatct tatctgataa tatttatccc
     1321 tatgataaac ctgtagatct ggttgtgacc agtcagaagc tcttgccttt cttaaaagaa
     1381 ctcggtgttt ttcccaaaga gacccgcttg tttggtttga gtttggactg tatccaacag
     1441 cagcgcgaag atttaatcaa aaccatctta gccttgcacc agaaccacta ccaaaaacgt
     1501 ttagaagaac tttggagagt tccttcttaa ttttttgaca gtccctccta gatactttta
     1561 cagtctaggg gggtctttaa ggttgtcact tgcaaccttg ataagctctc tttttaacca
     1621 ttgctatttg cttagaaaca gaactggaac tgttttagcg gttcaactcc tcttgtagga
     1681 tcttcttccc ttttttgaag gttttatctg aaatttggca taataaagtg cgctcccatg
     1741 ttctggaagc gcactttatt atcgctttta cttaaccttg tatccacttg atgatggaag
     1801 tgccgcccca attcttagat tgcttggcta acttttaagc tcttgtacaa ggaagaagtc
     1861 tttttgacta tctgttgatt agaggcgagc attaagctct gctgataatg cttcaaaacc
     1921 tggttttcca agaagcgcaa acatattacg tttgtaagct tcaacacctg gttgatcaaa
     1981 tgggttaaca gcattcatgt agcctgaaac agcgatggca agctcaaaga agtaaatagt
     2041 gtaaccaagg gtaaactcgt cttgagctgg aagagtgaca aacatgtttg gcacgccacc
     2101 atctgtgtgg gcaagaagca caccatctgt ggctttctta ttaacaaaat caacgtcttt
     2161 accttgaagg taaccaagac catctaggtc ttctgctaat tcaggaatga taacattttt
     2221 acgagggtta tcaacacgaa taactgtttc aaagaggtta cggtaacctt cttggataaa
     2281 ttgaccaagt gagtgaaggt ctgttgagaa gttagctgaa gttgggtaaa tccctttttg
     2341 atcttttcct tctgattcac cagctaattg tttccaccat tcgccaaagt actgaagaga
     2401 tggctcgtag ttagccaaga tttcagtgat gtagccttta cggtaaagca cattacgaac
     2461 agcagcgtat tggtaagcaa tgttttcaga gattttgtct gaagaaaggt ctttacgagc
     2521 agcatttgcc ccttccataa gagcagtaat atctgcacct gatgcagcaa ttggaagcaa
     2581 accaacagca gtcaagactg agaaacgacc accaacatta tctggcacca caaatgtttc
     2641 ccagttgtta gcgtcagctt ctaacttaac agcaccctta actttgtctg tggtagcgta
     2701 gatacgtttg ttagcttctt cttggccata ttttttaacc aaaagttctt tgaacacgcg
     2761 aaaagcaata gctggctcag tggttgtacc tgatttagag atcacgttta ctgagaattc
     2821 tttgtcttga acgtactcta caaggtcagc taagtaagtt gatgaaatag agtttccagc
     2881 gtaaagaatt tgtggggctt tgcgctcttt ggcagtttgc aagttagcaa aatgatgatt
     2941 taaaaaatca atcgctgctt tggcaccaag gtatgaccca ccgataccga tcaccacaag
     3001 aacttcgctg tctgccttaa tcttttcagc agcagtcaag atacgtgcaa attcctcttt
     3061 gtcatagttt tctggaagat caagccagcc caagaaatct gaacctggtc cagttccttc
     3121 acgcaataac ttgtcagctt ctgtgacttg gccttgtaaa aaatcaattt catgttgtcc
     3181 cgcaaatgac tcgaggactt ttgaataatc aaatgtaata tgtgacatga gtttcgctcc
     3241 cttaaaattt taagtttcat tagtatgata accttttcta tctaatattt caatggtttt
     3301 tagtggtttt cacctgtgaa aaagctaggt tttttgaaaa ggttttcgtt ttttcagaca
     3361 taaaaaatgt gtcaatgcaa gcatattctc atcatagtgg ttttttaaga gaaaatagga
     3421 tttcatattt cctaaaaata tcatgaaaaa aggccctgag gccgctttac cgtaataact
     3481 tattttaagg attttatcat ttcaacacgg gtaataaccc catcatgcat atgatcaatg
     3541 cataaagcca cacctacaaa ggcaattgaa gccccaatca cctgtaaata ctttctcatg
     3601 ctatcttctt aacattactt actctcatta tctaaaaatg gcaatcattt gaatacttaa
     3661 aaagcaaagg ggggagaata gcactagtgc gtttttaagt agatatcaaa atga
//
LOCUS       DATUYR010000099         3561 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.099, whole genome shotgun sequence.
ACCESSION   DATUYR010000099 DATUYR010000000
VERSION     DATUYR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.099"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(148..2313)
                     /locus_tag="VCI33_001615"
     CDS             complement(148..2313)
                     /locus_tag="VCI33_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP2832520.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDISNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(2300..2908)
                     /gene="recU"
                     /locus_tag="VCI33_001616"
     CDS             complement(2300..2908)
                     /gene="recU"
                     /locus_tag="VCI33_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEP2832521.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            2989..3504
                     /locus_tag="VCI33_001617"
     CDS             2989..3504
                     /locus_tag="VCI33_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEP2832522.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
BASE COUNT         1080 a          610 c          707 g         1164 t
ORIGIN      
        1 gtggtaagtc atttgtgtca tataaaaacg agtcctcaca gaacgtggac tcgcttttta
       61 tttagattat cacttaaggt agatattaaa agtaactaaa tggatatgta ataaaaggta
      121 ggtacagaca ataagtagag ttagagatta atgagttgtc tttttatcat cggtagatgg
      181 atgattgcta ttgttgtctg tattggttga attacttata tcattgctac tactagtatc
      241 atctgaagtc gtttggctag aagctgttgt cgtgttattg ctgtaaagat tgttgtaaac
      301 cgaattagta tagtcggtgt tgctcgcata ggttccgctt aagtagagga atccaccact
      361 gcgataaaga ccatttggca tcgtccaatc ttcactgtaa ccatttgtta agtaagtcat
      421 catgctacga taaacgtcag atgcaatctc tagacttgat ccgtatactg gggtcaagcg
      481 gtttttgtaa cctgtccaaa cagccatggc gtagcgctta gtaaagccaa caaagttttc
      541 gtctggcgct aatgtaccaa cataatctgg ataaaggccg tatttttcac caattttagc
      601 tagttcctca tcagtgtagt tagaagtccc tgttttacca gcttgcgcaa caccaggaat
      661 ggcagcagca gtacctgtac cataagtgag aacagttttt aacatatctg tcatcatata
      721 ggccgtggtt tctttcatgg cacgttttcc tttttcatca aaagttttac ttgtaccatc
      781 actaaattcc actttattga cataccttgg tttatgataa ataccaccat ttgcaaaagc
      841 agcgtatgca gcggccattt tttcactact tgcaccatat tttttatctg agctactgtt
      901 attacttgag atagcgtttg agtagtgcat ttcgggatag ttaataccta aactgcttaa
      961 gaaagatcga gcatagtcaa gtcctgcggc ttccagtgct cggactgctg ggacatttcg
     1021 cgatagcata atagcagctt ggattgtcat ccatccgtta tatctaaggt cccagttaaa
     1081 caattgggta gtggttccag gccaataata gactgagtca ttagtcgact gagcagtaga
     1141 agtataaaca ccagattcaa tagcaggagc ataggctgtg attggcttca tggtagaacc
     1201 ccagtcacga tcagttaaaa cagcttggtt agtcccaaat gaaacatttt catcttgatt
     1261 acgtccgcca agctgagcaa taacatgacc atttgtcaca tcaacaatcg ttgaagcaac
     1321 ttggaaatct tggtctggat agtaaacata atcaccagaa tgataaatat tataaagagt
     1381 ctgctgcgca tcggggataa tattggtata aacttttaaa ccagcggtaa aaatatcttt
     1441 attcgtttct tttttgactt cttcaataac ttgttttaga taattatcca tatattttgg
     1501 ataagttgag cgctgttgga gtgattgtag accttcagcg acgggagttg cgatggcagt
     1561 ttcatattct gctttcgtca gatgtttttc catgtacatc tgttgcaaca cgacgttacg
     1621 gcgattttga gcagcttcag gatgaaggta aggatcatat tgactaggag cttgagggat
     1681 tccagccaat agggctagtt gggcataaga taaatcctta agatccttgc cataataaga
     1741 cttagcggct gtcagcatac catagttgcc attacccata tatactttgt tgatgtaaaa
     1801 agtcaggatt tcttgtttag tatattttcg ctccatttga agagcaagcc aaacttcttg
     1861 agccttacgt tttaaggttt gatcagattc attagtagaa aaataggcta gtttaatgag
     1921 ttgctgatca agcgttgacc ccccttgagt ggtctgactc gttagattat gaaaggcagc
     1981 accaaaaata cgataaagat ctactccacg atggttaaag aaacgtttat cttcaattga
     2041 ggtaatagca ttaactagat taatagggat actatcagct gttacatttt cacgcttttc
     2101 agaacccaaa tcagcaatca gattgttatt accgtcataa accaagctag agtttgttga
     2161 ttttaactgg gcttctgaca gtttcggagc actgctaatg tagaaggtaa acaaaagacc
     2221 accaataata ataacaagga taataaggct aagaattgca cttaatacat actttagcca
     2281 cttaaggatt tttggatttt taattgtaat caccgcctaa aaaattttgt tcaattatgt
     2341 ttaaataagg gacttgtgga aaggctccaa atgctacctt aaagccattt tttctgatat
     2401 aatcaatggg catcgattta ctgccattat ctatctgata aaatgaaatc aaggcttgag
     2461 caggtaggta gtaagtctcc ttcagtgtgg aaaagtgaag aaggacaaag cagattcctt
     2521 tttgatgtaa aacgcaagcc atatgttcaa tctgatgtaa atggaaattt ttcatgggca
     2581 tagctgtttt ttgcctggtt tcttttgctt caaaatcaac ataatgtcct ttataaacgc
     2641 cgcaatagtc agtcgttgaa gcttgtctaa agtacgcttc aacgattttt gcccgactgc
     2701 gtttagggta atccactttg acaatttgga caggagtagg ttttttgtga atgacagcta
     2761 tttgacgaga taaataataa tcatttgtcg cgttgatagc agcttcaaag gacatacctc
     2821 tattggcgaa gtccacctta ttttgctttg tttgcttttg aacggaactt actttttgtc
     2881 gaataagatt atgaggataa ttaaccattg ttctcctaat attatctagt taatgctaaa
     2941 aatttaagca cttttattat aacatatttt ttgaaaggat aacacctaat gactgcgatt
     3001 ctaattacag gttaccgaag ctttgaaata gggatttttg accataaaga cccacgagtt
     3061 tcaattatta aacaggctat tcgtaaagat ttaataggtt atttggaaaa tggtgtggat
     3121 tggtttatct ttacagggaa cttgggtttt gaacaatggg ctttggaagt agctaatgag
     3181 ctaaaagagg agtatccctt gcagatagct actatattct tgtttgaaac gcatggagat
     3241 aggtggaatg aaaaaaatca agaagtttta agtcagttta gggctgttga ctttgtaaaa
     3301 tattactttc caaattacga acaaccgacg caatttagtc aatattatca gtttttatta
     3361 gaaaagacag aaggggctta tgttttttat gacacagaaa acgagaccaa cttaaaatat
     3421 tttctcaaga aagcaaaaga tatgccgcat tatcagttgc tattgctaac ctttgatcgg
     3481 ctaaatgata tgtcacaaag ctagcattgt atttttaggg gtattggtat ttaaatactt
     3541 gctttttgaa ctatttttct g
//
LOCUS       DATUYR010000100         3178 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.100, whole genome shotgun sequence.
ACCESSION   DATUYR010000100 DATUYR010000000
VERSION     DATUYR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.100"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(24..503)
                     /locus_tag="VCI33_001618"
     CDS             complement(24..503)
                     /locus_tag="VCI33_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832523.1"
                     /translation="MIAIIRTHEKGRSQFLCELDIMQFTENQVRDHMIERGIKDDAFF
                     ICGFSDWNVDRIMSLSEVYLLKRCIVGLYDGDDYIVQYLLKKGLSVPAIVTKFYVFLS
                     KDEKEAMGYVLKNVSFDSLIDFWQRSVTWANALNAYIQSGILLNTSKGFYVLKTEGG"
     gene            complement(511..992)
                     /locus_tag="VCI33_001619"
                     /pseudo
     CDS             complement(511..992)
                     /locus_tag="VCI33_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            complement(1045..1317)
                     /locus_tag="VCI33_001620"
     CDS             complement(1045..1317)
                     /locus_tag="VCI33_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832524.1"
                     /translation="MSNTRSEADKKLLVVTQELSELLISHQYDQSWEKAGELNSLLKK
                     REELTLPGYMVDMIQQHLKSYYYQNNMINKAHKSMSAIGHKLQEFH"
     gene            complement(1450..1655)
                     /locus_tag="VCI33_001621"
                     /pseudo
     CDS             complement(1450..1655)
                     /locus_tag="VCI33_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057932.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1706..2083)
                     /locus_tag="VCI33_001622"
     CDS             complement(1706..2083)
                     /locus_tag="VCI33_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832525.1"
                     /translation="MFLENEFNYFISTRNNLELVIDSLVLMIPDREFYYPEIQTGELR
                     DYQIDIYDLIKIGYVGVYEIQKDYEDKLRELADFKRKLLKFGLLMQPLEKQKEIVIRL
                     AAKYHLEKRILMKKEMFRDEEVD"
     gene            complement(2084..>3178)
                     /locus_tag="VCI33_001623"
     CDS             complement(2084..>3178)
                     /locus_tag="VCI33_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEP2832526.1"
                     /translation="KTHFGYLTDNDSSRLQVTIDYVRITLKSVRDLEFFCRNFLHCRF
                     KEFSSVESKLMNYNHIWKRGDIWIFDYADKHDTGNYQITLQLSGQGCRQLELLMEQEN
                     FAWLDWLIYLRRSYRDEMNVTRFDLAIDELYLGKDRENEHFLLSDMIAKYYHHELAFE
                     SLRTWNYIGGGALNFNDMEEIEQNRQGISLYFGSRQSEMYFNFYEKRYELAKLEGISV
                     EESLEIFEIWNRYEIRLSQSKANAVVDEFISGIPLGEIARGLINSKIQVYDGKNKYGA
                     FMADQKWQMMFGGVEPLTFVTRPESYNIERTIRWLIHQVSDSLAMVSEYDSMIDGDNL
                     QMIINSGEVNERGEKILETIRYNLGLDKGA"
BASE COUNT         1059 a          673 c          341 g         1105 t
ORIGIN      
        1 tttattttca ctaaataatc catctaacct ccttctgttt ttaacacata aaatcctttg
       61 gatgtattta acaatattcc actttgtatg taagcgttta aagcatttgc ccaagttact
      121 gaccgttgcc aaaaatcaat caatgaatca aatgatacat ttttcaaaac atatcccatt
      181 gcttcttttt catcttttga caaaaagaca taaaactttg tcactatggc tggcactgac
      241 aagcctttct ttaacaaata ttgaacaatg taatcatcgc catcataaag accaacaata
      301 catctcttta aaaggtaaac ttccgaaagt gacataatcc tatcaacatt ccaatcagaa
      361 aaaccacaaa tgaaaaaggc atcatccttg atacctcttt ctatcatgtg atctcttact
      421 tgattttccg taaactgcat gatgtctaac tcacataaaa attgacttct acctttttca
      481 tgagttctta taattgcaat cattatctac ttaccctgat atttcgacat atccgtagtc
      541 tgaaaaacca ccttggcttt ctaagtcaat atctcgacca taagcttcac aatcaaaata
      601 gagttccata ttttctttac caacaccttc tgaacctaat tcattaaacc agtattcgcc
      661 taattctcgt ctagttgtga catcaggcag taaacaacaa tcaccaaaat taaaatcata
      721 atctacaaag tctgaggcag tataacctgt tacctgatgc actgcttgaa caagttcact
      781 atcaacattc tctaccagtt caacaaatta ttaatcatct caatagaatc atgttctttt
      841 agtaactgac aatccgaatc tgcaataata tactctaaat catcttcttc acgaagcccc
      901 aaagctaact taagctcact catttccaca ggaaaacaaa ctaacgtagc tctaccagtt
      961 tttatatctc taacaattgc actaacatcc atttttgaca ctccttaatt aaaaaaggag
     1021 caagccattg acttgctctc atccttaatg aaattcttgt agtttatgac caatcgctga
     1081 catagactta tgagccttat taatcatatt attttggtag tagtatgatt tcaaatgttg
     1141 ttgaatcatg tcgaccatat aacctggtaa ggtcaattct tcacgttttt taagtaagct
     1201 atttaactct ccagcttttt cccatgactg gtcatactga tgactaatca gcagttctga
     1261 tagctcctga gtaaccacta acaacttttt atctgcttct gaacgtgtat ttgacatttt
     1321 tcttttcctc tttttctttt tttatactca ataccaataa atcaacatat gatagttgga
     1381 cacctaattt tcatcatttt ccctcataaa aaagaacctc ttaaggttca tgaatctaaa
     1441 actaactgtt taagcatctg aaatacctaa taaataattt ggagtagtat ttaatagttt
     1501 agatagctca attagttttg aaaacttggt tctcgctttc cgttctccca atttgaatat
     1561 gctcctctat taataccaat tctatcagca agctcttgtt gtgttaattt attaagctgt
     1621 cgttgttttc ttaaacgatc tccaagtttt tccatttacc ttacccctta tgatttataa
     1681 gttggatttt aaatgatccc agaaattaat caacttcttc atctctaaac atttcctttt
     1741 tcatcagaat tctcttttcc aaatgatatt tagcagctaa acgaataact atctcttttt
     1801 gcttttctaa aggttgcata agcaaaccaa actttaaaag tttccgttta aaatcagcaa
     1861 gttcccttaa cttatcttcg tagtcttttt gaatttcata aacaccaaca taaccaatct
     1921 taatcaaatc ataaatgtct atctgataat ctctaagctc acctgtttga atctcaggat
     1981 aataaaattc acgatcagga atcattaata ccaaagaatc aatgaccagc tctaaattat
     2041 ttctcgttga tataaaataa ttaaattcat tttctaaaaa catctacgcc cccttatcta
     2101 aacctagatt atatcgaata gtttctaata ttttttcacc tcgttcattc acttcaccag
     2161 aattaatgat catttgtaga ttatccccat caatcatact gtcatattca ctaaccatag
     2221 ctagagaatc agatacttga tgaatcaacc agcgaatagt acgttcaata ttataagatt
     2281 ctggtctcgt gacaaatgtt agaggttcaa cacctccaaa catcatctgc cacttttgat
     2341 cagccataaa tgcaccatac ttattttttc catcataaac ttgaatttta ctattaatca
     2401 atcctctcgc aatttctcct aatggaatac cagaaataaa ttcatctacc actgcattcg
     2461 ccttagattg agataatcgg atttcatacc gattccatat ttcaaatatt tctaaagatt
     2521 cttcaacaga gatgccctct aatttagcta attcatacct tttctcataa aagttaaaat
     2581 acatttcaga ctgtcgacta ccaaaatata acgaaatacc ttgacgattc tgttcaattt
     2641 cttccatatc attaaaattt aaagcaccac caccaatata attccatgtt cgcaaacttt
     2701 caaaagctaa ctcatgatga taatatttgg caatcatatc cgataaaaga aagtgttcat
     2761 tttctctatc cttacccaga tacaattcat caatagctaa gtcaaaacgt gtaacattca
     2821 tttcatctcg ataagaacgt cttaagtaga ttagccaatc taaccaagca aaattttctt
     2881 gttccataag taattctaat tgtctacatc cttgaccaga tagctgtaat gttatttgat
     2941 agtttcctgt atcatgttta tctgcataat caaatatcca aatatctcca cgtttccaaa
     3001 tatggttata attcatcaac ttagattcaa ctgatgaaaa ttctttaaat cgacaatgta
     3061 aaaaattccg acaaaaaaat tccaaatctc taacgctttt taacgtaatc ctgacatagt
     3121 caattgtcac ttgtaatctt gaactatcat tatcagtcaa ataaccaaaa tgtgtttt
//
LOCUS       DATUYR010000101         3164 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.101, whole genome shotgun sequence.
ACCESSION   DATUYR010000101 DATUYR010000000
VERSION     DATUYR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.101"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..835
                     /locus_tag="VCI33_001624"
     CDS             <1..835
                     /locus_tag="VCI33_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEP2832527.1"
                     /translation="GNAGQVSQVESGKRPITSSMLVYLNALTASSYTYIVFGELDEFI
                     ENLFHYFFSSILYRDLEAVDENLYSFMSDDLISIQSSCLSIAKTFANFNIQRKRFMIS
                     TETEMDTFHKKDDIDVWVGGKSYNPARSFRNNPINELTVIDFEEMADILLLMLRDNLI
                     RSFEINVCNTLFELDKNGEPTTFNLDKIDSIINKWWSENVSTEIIPNLIKKLRENPLF
                     NIGFMVNDILERMYKEDIPKSYLTSVPLVISKKARSTLSIKSNGSQKIDKSKFAQILS
                     L"
     gene            901..1653
                     /locus_tag="VCI33_001625"
     CDS             901..1653
                     /locus_tag="VCI33_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP2832528.1"
                     /translation="MAIPDLDKFSSNLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            1650..2252
                     /locus_tag="VCI33_001626"
     CDS             1650..2252
                     /locus_tag="VCI33_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832529.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(2728..3009)
                     /locus_tag="VCI33_001627"
     CDS             complement(2728..3009)
                     /locus_tag="VCI33_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP2832530.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRIALRIERFKKTYDVQTYR"
     gene            complement(3016..3066)
                     /locus_tag="VCI33_001628"
                     /pseudo
     CDS             complement(3016..3066)
                     /locus_tag="VCI33_001628"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         1039 a          594 c          523 g         1008 t
ORIGIN      
        1 aggcaatgca ggtcaagtta gtcaagttga aagtggtaag cgaccaatta cgagttcgat
       61 gttagtttat ttaaatgctc taaccgcttc gagctatacg tatatcgttt ttggagagtt
      121 agatgagttt attgagaatc tgtttcatta ttttttcagt tctattttat atagagattt
      181 agaggctgta gatgaaaacc tatattcttt catgagtgat gatttaattt caatccaatc
      241 aagttgtcta agtattgcca agacatttgc aaatttcaat attcaaagaa aaagatttat
      301 gatttctact gaaactgaaa tggatacttt tcacaagaaa gatgacattg acgtatgggt
      361 tggtggaaaa agttataacc ccgctaggag ttttaggaat aatccaataa atgaattaac
      421 tgttattgat tttgaagaaa tggctgatat tcttttgttg atgttgagag ataatctaat
      481 taggtcgttt gaaattaatg tctgcaacac actgttcgaa ttagataaaa atggtgaacc
      541 gacaacattt aatctagata aaattgactc aataatcaat aagtggtggt ctgagaatgt
      601 ctcaacagag ataataccca acctcataaa aaaacttaga gaaaatccct tgtttaatat
      661 cggatttatg gtaaatgata tattagaacg aatgtataaa gaggacatac caaagtctta
      721 ccttacttct gttccattag ttatttccaa aaaagcaagg tcaactttat cgattaagtc
      781 aaacggaagt caaaaaatag ataaatcaaa atttgcacag attttgtccc tttagagacg
      841 gatgacttac acattcaacg tggattattc ttcccttgga gaaaggggat ttcagaacgc
      901 ttggcgattc ctgatttaga taaattttca tctaacttac aacaggggaa aattcctgtc
      961 ataaagcact tcgaactcaa tttagataag gggagtcact gggctattgc tggaaattca
     1021 ggttctggga aaccgtacgc tctcacttac ttcttgagtg tcctgaaacc tatgtccggc
     1081 ctaattatca ttgacccaaa attcgatacc cctagccaat gggcaagaga aaataaaatt
     1141 gccgttatcc acccagtaga aaaccattca aaatctgatt tcgtttcaca agtcaatgaa
     1201 caattaaacc agtgtgcaac tctcattcag aaacgacaag ctatcttgta tgataatccc
     1261 aatcatcaat ttacccatct aactattgtt attgatgaag tcctcgctct atcagaggga
     1321 gtcaataaga atatcaaaga agcttttttc tccctactat cacaaattgc cttgttggga
     1381 catgctacta aaatccatct attcttggga agccagcgtt ttgaccataa cactattccc
     1441 atttcagtga gggaacagct aaacgtatta ttgcaaattg gaaatatcaa tcagaaaacc
     1501 actcaattct tatttccaga cctagatcca gaggggattg tcattccaac aggacacgga
     1561 acaggtatca ttcaagttgt cgataatgaa cactcctatc aagttctacc cttactctgt
     1621 ccaacctact atactaagcg aggtatccta tgaccactaa aacctacttc atgagaagct
     1681 tcaatctcat attatactta ctgcttattg ctccaatctg ctatgtgttc ggttggctgt
     1741 tcaatcttgt ttcagttcaa ttatctaccg tcacatttgt caatctggat acggttaaaa
     1801 acgttactga taccattagt tctgtatgtt atggattcgc tcttatttct ttacttctag
     1861 ccttattatc tggattaaag atgatacaac gcttgaatac agatagcctt gggaactact
     1921 gccaatctat ttatcgcacc tttctattaa gacacttcct tttccagcgt gaacgagtac
     1981 agaaggtaag caactttgaa caccagaccg ttaccactat caatccaatc cataacgggt
     2041 ttaaccgagc tgtacgtaag tgtatcgtag atatacgaaa agatgccatt actgtatttc
     2101 taaaagttcc tagagaccaa caggggcaaa aaatcctaaa ggatacggga gtacaactga
     2161 aagaagagat tgtcatccaa cacacagact attatttctc ttctcctgtt cgagttcgta
     2221 accaattgtg gtttattggt caaaagcgtt aattccttgg ggctgtggct tgcgttaagc
     2281 aaaagccaga cagccccttt tttatattct tatctatcat gacctattgg ggttactacg
     2341 acaacgaaag ttagaagctg tgggtattta tttatcttga aaagattgct gaactaacgc
     2401 aataactact ctaccattca ctctaccttt tattaagcaa ctatcaataa atattgatac
     2461 aataaggttt ataacagcta aaatagaaaa tacttaacct ttacagaagt aaaataattg
     2521 agatgagaaa agcctatgaa atgaacgttt caaaggcttt tttcgtgttt aaaaaaacaa
     2581 atgaaaaaac aaaatagaga gtagggttat taacactgtc aatcctgtgt acgcaataac
     2641 aatccatgct ttttgataag gatattttgc tttttcttta atcttatcaa tctgatctaa
     2701 gctctcaccc tttgctataa cttcaagtta tcggtaagtc tgaacatcat atgtcttttt
     2761 aaatcgctca attcttaagg cgatacgcat gattatcaac cataagatag caaccggacc
     2821 atatccccac caacctaacc aagacattat aggataaata ctgatagcaa ttaggagtgt
     2881 cccaaataac ataatatgcg tattcctatc acattgttta atctcctcat ccttaataat
     2941 taaagtcatt tttgggatat cttctgctat tagttgctct aaaggaacct gtaaggactt
     3001 acttaacatc aaaagatttt gcaggtctgg atagctttta ccgttttccc agtttgagat
     3061 agtcttcttg aaacatacat catttcagca agctcttctt gtgacatctg taattttttt
     3121 caataataag ataactggtt tttaaagccc ataataaatc tcct
//
LOCUS       DATUYR010000102         2975 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.102, whole genome shotgun sequence.
ACCESSION   DATUYR010000102 DATUYR010000000
VERSION     DATUYR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.102"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            67..672
                     /locus_tag="VCI33_001629"
                     /pseudo
     CDS             67..672
                     /locus_tag="VCI33_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527535.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            685..1494
                     /locus_tag="VCI33_001630"
     CDS             685..1494
                     /locus_tag="VCI33_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEP2832531.1"
                     /translation="MDSKALNKQNLSRSKKILLNISDNPKNPKFYKLSRQELERAKIF
                     KTENLIKSQKYQRYKRGTIVFIHFGVNIGNEFSDSHFGIVLNKKDHPNNGKLTILPLT
                     SKNSKESLSINKEIFTSIMDDAEKTVQTIQNVLNLTTEVERIHHSLPVPPAFFQIKKE
                     NKYHDIWMKYYNRHDPKGIHVPVANITVRKWIQSDLDKINFLKKRYANYDKVSYAKLD
                     SITSVSKLKIAKPINDLDPVGKITLSKEIMDNIDKALARQLLSGQWGKLDN"
     gene            1744..2832
                     /locus_tag="VCI33_001631"
     CDS             1744..2832
                     /locus_tag="VCI33_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP2832532.1"
                     /translation="MKIKSYKKENGETAYKFLLYAGYVNGKRKYIRREGFKTKQAARE
                     TLISLQAELDKPKSSMTFGVLTKQWLNEYEKTVQGSTYLKTERNINKHILPKLDKVKI
                     GDINPLLIQRLTEEWCNDLKYGGKILGLVRNILNLAVRYGYINNNPALPITPPKIKRK
                     RKMNNNFYTLDQLKQFLELVEKTDNIEKIALFRLLAFTGIRKGELLALTWDDLNGNTL
                     SINKAVTRTQVGLEIDVTKTKSSDRLISLDDETLEILQELHETFPTSTLMFQSESGGI
                     MTPSLPRKWLLQIIKGTDLPQITIHGFRHTHASLLFESGLSLKQVQHRLGHGDLQTTM
                     NVYTHITQSAIDDIGTKFNQFVTNKQLD"
BASE COUNT         1102 a          530 c          512 g          831 t
ORIGIN      
        1 ctaactggat ttaaacaaaa tacaatttct aatcacgaaa atggtaatag acaacttgac
       61 gaagtagata ttcgaaaata tgctaaagca ctgaaaatag agccacaaca gctttttgac
      121 tattcctctt ctcccacaaa cccccaagtt gaactcatcc catctaccct acaaaaaata
      181 aactctactt cttctcaatt agaacacagt agacagataa ttgttttaga tacagctgag
      241 actttattgg aacaacagaa agaaattaaa aacaacgaag atactattgc cgaattattt
      301 tcttacaact actacgacca cgcagcttca gctggtacag gtcagtatct aaatgatgta
      361 caagtagaaa aaattgagtt accagtcgat tatgacgcag actttgttat ccctgtttat
      421 ggtgattcca tggaaccgaa gtatcactct ggggattatg tatttgttaa gctatccgta
      481 gagcttacag atggcgatat aggcgtcttt gaatactatg gtgacgctta tatcaaacag
      541 ttgcttataa acgctgaagg ggcgttttta cacagtttaa atcaatgcgg tgattatcca
      601 gatataccga tagatagaga tagcgacttt aggattattg gtgaagttat ggggagttat
      661 agggagagat aggcaggagc agaaatggat agtaaagcat tgaataaaca aaatttaagc
      721 cgtagcaaga aaattctgtt gaatatctct gataatccga aaaatcccaa attttacaaa
      781 ttatcaagac aagaactcga gcgcgctaaa atttttaaaa cagaaaactt aattaaatct
      841 caaaaatatc agagatacaa acgaggaact atcgtcttta ttcattttgg agtaaatatc
      901 ggcaatgaat tttccgattc gcactttggg attgtgctga ataaaaaaga tcatcccaat
      961 aacggaaagc taactatctt accgctaaca tcaaaaaact ctaaagagag tctgtcaatt
     1021 aacaaagaga tttttacgag cataatggat gatgccgaaa aaacagttca aacaattcaa
     1081 aatgtgctaa acctaacaac tgaagtagaa cggatacacc actcgttacc agttccccca
     1141 gcgttttttc agattaaaaa agaaaataaa tatcatgata tatggatgaa atattacaat
     1201 aggcatgacc ctaaagggat tcatgtgccc gtagcaaaca ttaccgtccg aaaatggata
     1261 caatcagact tagacaaaat taacttcctc aaaaaaagat atgcaaatta tgacaaggtg
     1321 tcctacgcca agctggactc aatcacttct gtcagtaaac taaaaatcgc taaaccaatt
     1381 aacgatcttg accctgtcgg aaaaataaca ttgtctaaag aaatcatgga caacattgat
     1441 aaagctctcg caaggcagtt attatctggc cagtggggga aacttgacaa ttaaatatca
     1501 ctatgttaga attaaggtgt aatcttggta gcctcgctac cactgaaaca ttattttgga
     1561 gtgtccaact ccactgtgag cgcctgtttt cgaataagcg ctcttttttg ttttaaaaaa
     1621 ataaaaaagc cccacgctct caaagtttgg cgactctgag cgtgaggcaa gacagtataa
     1681 gaaacaacca ttaaaaaggt cattttcttg tacctatttt atcaaattga aagatggtat
     1741 gcaatgaaaa ttaaatcata taaaaaggaa aatggtgaaa ctgcttataa atttcttttg
     1801 tatgccggtt atgttaatgg aaagagaaaa tatattaggc gagaaggttt caaaactaag
     1861 caggctgcaa gggaaacctt aattagtttg caagctgaac ttgataaacc taaatcaagt
     1921 atgacgtttg gtgtgttaac taaacaatgg ctaaacgaat atgaaaaaac tgtccagggc
     1981 agtacctact taaaaacaga aagaaatatt aataaacata ttttgccaaa acttgataaa
     2041 gtgaagattg gagacatcaa cccactactt atccagcggc ttactgaaga atggtgcaac
     2101 gatttaaaat atggaggaaa aattcttggg cttgttagga atatcttaaa tctagctgtt
     2161 agatacggat atatcaataa caatccagct ttgccaatta cacctccaaa aataaaaagg
     2221 aaaagaaaaa tgaataataa tttttataca cttgatcaac ttaaacaatt ccttgaacta
     2281 gttgaaaaaa ctgacaacat tgaaaaaata gccttgttta gattattagc atttactgga
     2341 atacgaaaag gggagcttct ggcactaact tgggatgatt tgaatggtaa tactctatca
     2401 attaataaag ctgtcacacg tactcaagtt ggactagaaa tagatgttac gaagacaaaa
     2461 tcgagcgata gattaatcag cttagatgat gaaactttgg aaattttaca agaacttcat
     2521 gaaacttttc ctacttctac tcttatgttc caatctgaat caggtggaat tatgacgcca
     2581 agtttaccac gaaaatggct attgcaaatt atcaaaggga cagacttacc acaaatcaca
     2641 attcatggtt tcaggcacac tcatgcaagc ttacttttcg aatcaggtct atccttgaaa
     2701 caggtgcaac ataggttagg gcatggagat ttacagacaa ctatgaacgt atatactcac
     2761 atcacgcaat cggcaattga tgacattgga actaaattca atcaatttgt tactaacaag
     2821 caactagatt gacaactaat tctcaacaaa cgttaattta acaacatcca agtaactccc
     2881 accagctcca tcaatgctta ccgtaagtaa tcataactta ctaaaacctt gttacatcaa
     2941 ggttttttct ttttgtcttg ttcatgagtt tccgt
//
LOCUS       DATUYR010000103         2824 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.103, whole genome shotgun sequence.
ACCESSION   DATUYR010000103 DATUYR010000000
VERSION     DATUYR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.103"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            102..752
                     /locus_tag="VCI33_001632"
     CDS             102..752
                     /locus_tag="VCI33_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP2832533.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            755..1423
                     /locus_tag="VCI33_001633"
     CDS             755..1423
                     /locus_tag="VCI33_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2832534.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            1432..2664
                     /locus_tag="VCI33_001634"
     CDS             1432..2664
                     /locus_tag="VCI33_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP2832535.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPGTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSVMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
BASE COUNT          945 a          408 c          560 g          911 t
ORIGIN      
        1 caaacgataa agatcgttta cagttatata gttagaaata aagataaccg atcattgtag
       61 agatttaagc atttgttgag aaaatttgtt ataataagcg tatgaaagaa agactagaac
      121 attattctac cagctctata tatagcggat ttgaagtgta cttgccgttg gtactacaga
      181 caataacaga tgtgattatt gctgagaata tcaaaagcaa aaaagaaaca gggtttaaac
      241 tatacgaaca ctttacatcc cgtattaaat cggaagcttc gatgattgaa aaatgtcaac
      301 gcaaacaact tcctctaaca agcaaatctg cattaaaaat cattaaagat agtatcggta
      361 ttcgcattat ttgtggtttt attgatgata tttatcggat ggtagacttg ctcaaaagca
      421 ttcctggtat gtcagttaac actgaaaaag attatatttt aaatgccaag cctaatggct
      481 atagatcata tcatctcatt ttggaattag aaacacattt tccagatatt ttaggggaaa
      541 aaaaaggatg ttattttata gaagtgcagt tgcgaaccat tgctcaagac tcttgggcta
      601 gcttggaaca tcaaatgaag tacaaacatc aagttgctaa tgcagaaatg attacaagag
      661 agttaaagcg ttgtgcagac gagttggcat cttgtgatgt taccatgcaa accattcgtc
      721 aattgattca agaaacgacg gaggaagagt aaccatgaaa attttgttag ctgaagacga
      781 atggcagatg tctaatgttt tgacaactgc catgactcat caaggttatg acgttgatgt
      841 ggtttttaac ggtcaagagg caattgataa agctaaagac aatgcatatg atattatgat
      901 tttagatatc atgatgccga ttaagtctgg cattgaagct ttaaaggaga ttcgcgcttc
      961 aggaaattgc tcacatatta ttatgctgac ggctatggca gaaattaatg accgtgttac
     1021 tggactagat gcgggagctg atgattatct gactaaaccg ttctcattaa aagaactttt
     1081 agctagatta aggtcgatgg aaagacgagt agaaagtttt actcctcagg tattacaatt
     1141 tgctggtgtc acattaaata ttaatgagca agaattgagt gccggtaatg ctatacgatt
     1201 agcttctaaa gaaggtaaat taatggcctt tttaatgtta aatcaaggaa agtacttgga
     1261 tactaaaaca ctttaccaac atgtttggtc agaccaagaa gattatgata cttctattgt
     1321 ttgggtttat atttcttatc ttcgtcaaaa attattagct attcaagcaa atgtcattat
     1381 tgcaggtgat aaagatacta gctattgttt attagaaaaa taggaggtga catgtttaat
     1441 cgtatccgta ttcgttttat catgattgct tctattgcca tttttattat tttaagttca
     1501 attgttggta ttattaacac ggcacgctgt taccaaagtc aacaagaaat taaccgtatt
     1561 ttacatttaa tttcctcaaa taaaggtaaa ttacctggca ctacggaaag ctctaaacga
     1621 cttggaacga aactgtctga agatagccta agccagtttc ggtattatag tgtcattttt
     1681 aatgctaatg gacatctact ttcttctaat actgctaata tttcagcttt agatagagaa
     1741 gaagcccaat attttgctag actttttgct aagtctgggg aagaaaaagg cagttaccgt
     1801 caccaagata gtgtttattc gtacttgatt actcagctgc caaatgaaga aaaactggtt
     1861 gtaattttag atactacctt ttattttcgt agtgtaggag atttattagc tgtttcagtg
     1921 atgttggcct ttggaggatt tatctttttt gttgttttag tgagtctttt ttccggtatg
     1981 gtcatcaaac cttttgtcca aaattatgag aagcagcgtc gctttattac taatgcaggc
     2041 cacgagttaa aaacaccact agccattatc tcagccaata atgaattggt cgaattaatg
     2101 actggagaat cagaatggac caagagtaca agtgatcagg tgaagcgatt gacaggcttg
     2161 attaatcaaa tgattacttt ggctcgtttg gaggaacagc cagatgtggt attacatatg
     2221 gtagactttt cagctattgc tcaagatgca gctgaagatt ttaagagtct ggttttgaaa
     2281 gatggtaaac gttttgattt gacgattcag ccgaatatta tgattaaagc ggaggaaaag
     2341 tcattatttg aacttgtgac aattttagtt gataatgcca ataaatattg tgatcctaaa
     2401 ggcttggtca aggtatcttt aaccactatt ggaagaagac gaaaacgagc gaaattagaa
     2461 gtttctaata cctatttgga aggaaaatcc attgattaca gccgcttttt tgaacgcttt
     2521 tatcgtgaag acgaatccca taatagcaaa gaaaaaggct atggaattgg tttatctatg
     2581 gcagaaagta tggttaagtt atttaaagga acgataactg taaattataa aaacgatgct
     2641 attgttttta cagtggtgat ttaacttaaa catttttcag tacctttagc tagtaataat
     2701 gtctaggcaa aggtaataaa tgatgcttaa ccagcccatg aggctggttt taacgtttaa
     2761 tttagtagaa gagtgattaa agaaggtagt ttgaaataag aaaatttttg ttatgatcct
     2821 aaaa
//
LOCUS       DATUYR010000104         2823 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.104, whole genome shotgun sequence.
ACCESSION   DATUYR010000104 DATUYR010000000
VERSION     DATUYR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.104"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1490
                     /locus_tag="VCI33_001635"
     CDS             <1..1490
                     /locus_tag="VCI33_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEP2832536.1"
                     /translation="LFYSEFENQLFETIMFLSMKTLVLDINHFSKEIENKSEAYEQCI
                     QQIREENGINHFFDRYPYLLKQINKEVGLIEESYSLLFDRFLEDLSEIRSCFNISEPL
                     SNVAFSLGDSHSKKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLP
                     KSLVKADYCWQLGVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDL
                     YLIDVETFFQRKLNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISG
                     KGGKRKKGKYELINKNRGDMKLVKVDYFQEDGFNIPTLNGKVVEPLDYANEIISGFRE
                     CYIFLLSQRSKIKGIVEGFPELKSRALFRNTSDYGKFLQASTNPKYLFSEKKKKNLFS
                     ILYEAKHIEHFIVDNEIKDLMNGDIPYFSMDTRGNVYNSVGTLIGNLGDTTSLFDSIT
                     ILNDERLKFTCELLEIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            1492..2025
                     /locus_tag="VCI33_001636"
     CDS             1492..2025
                     /locus_tag="VCI33_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832537.1"
                     /translation="MDKTDFRNISAFVSQDSPMFDGDVMYNISLGRESVSGEQVIETC
                     KRVSIYDDIRSMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALD
                     VKTERIIQKNVEALHCTRILVTHRLNTVEKADKILIMDNGKIIDYGNHHYLYKNNKDY
                     CDLYDSYMNKYQEEEVK"
     gene            2022..2759
                     /locus_tag="VCI33_001637"
     CDS             2022..2759
                     /locus_tag="VCI33_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832538.1"
                     /translation="MKQLVLKDVCKKYPNQLNYALDHINLIVEKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISAFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNVDSSKIDDLINNYAKRFNLYEQLKKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEGTMRILSEINKIEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHMEIDKEDCGETFYDVISQALSDRGE"
BASE COUNT         1019 a          367 c          527 g          910 t
ORIGIN      
        1 agttatttta tagtgagttt gaaaatcagt tatttgaaac gatcatgttc ctttcgatga
       61 aaacactagt tttagatata aatcattttt ccaaggaaat tgaaaataag tcagaggcat
      121 atgagcagtg tatacagcag ataagagaag aaaacggaat aaatcatttt tttgacaggt
      181 atccgtattt gctaaaacaa attaataaag aagtaggact gatagaggaa tcatattctt
      241 tattatttga tagattttta gaagacttat ctgagataag gtcgtgcttt aacattagtg
      301 agcccctaag caatgttgcc ttttcacttg gtgacagtca ctctaaaaag caaacagttg
      361 ttaaaattgc gtttaaagag aaatctgttt actacaagcc taaaagttat cattcacaca
      421 gtatcttgtt agaacttact agtttattga aatctagtaa tataccttca ttcagtcttc
      481 ctaaaagctt agttaaagca gattattgtt ggcaacttgg tgtagcttat acaagttcca
      541 ataaagatga agtagcgaaa atttacttta agtatggtgt tttagcagca ttttctgaaa
      601 tattttctat tacggactta catatggaaa atgtgattgt ttcaggcgga gatttatatt
      661 taattgacgt tgaaacattt tttcaaagaa agttgaatgt acaaaatcag aattttgaag
      721 ggattactgt agacacctat cagagaattt atgagacatc actttcaaac ggtttatttc
      781 ctgtacaatt tgagaaaaac agcgctccta acgtttctgg aataagtgga aaaggtggaa
      841 aaagaaaaaa aggaaaatat gaactgataa ataaaaatcg tggagatatg aagttagtaa
      901 aagttgacta ttttcaagaa gatgggttca atattcccac attaaatggg aaagtggtag
      961 agcctcttga ctatgctaat gagatcattt caggatttag agagtgctac atatttcttc
     1021 tatcacaacg ttcaaagatt aaaggaatag tagaaggttt tccagaattg aaaagcagag
     1081 ccctatttcg aaatacttcc gattatggaa aatttttgca agcttctacg aatccaaagt
     1141 atcttttctc agaaaaaaag aagaagaatt tattttcgat actatatgag gcaaaacata
     1201 ttgaacattt tattgttgat aatgaaatta aagatttgat gaatggtgat attccttact
     1261 tttcaatgga taccagagga aacgtctata attctgtagg gacattaatt ggaaatctag
     1321 gagatacaac ctcgttattt gatagtataa ctattttgaa cgatgaaaga ttgaagttca
     1381 cttgtgaatt gcttgaaata gtcttgaaaa aaccgattaa gcattgggag agggaaaagg
     1441 gcaaatctta tcagttttta tcaatctcct cagaacatta ccttctctaa aattgataaa
     1501 actgatttta ggaatatatc ggcttttgta tctcaagatt ctcccatgtt tgatggggat
     1561 gtgatgtata acatttcgct agggagagaa tctgtttcag gagaacaggt tattgaaact
     1621 tgtaaaaggg tatcaatata tgatgatatc aggagtatgc caatgaagtt tcatacccca
     1681 ctttttcgag ataatccatc actatctggg gggcaaaaac aacgaatttc tttagcaaga
     1741 gaacttgtaa ccacccctag aatcttagtt cttgacgaac ctacatcagc tttagatgta
     1801 aaaactgaaa gaataatcca aaaaaatgtt gaggctttac attgtacgag gattttggtt
     1861 acccatagac ttaatacagt tgaaaaagct gataagattt taataatgga taatggcaaa
     1921 attattgact atggaaacca tcattatttg tacaaaaata ataaggatta ttgtgactta
     1981 tatgactcgt atatgaataa atatcaggag gaagaggtaa aatgaaacaa ttagtattaa
     2041 aagatgtttg caaaaaatat cccaatcaac tgaattatgc tctagaccat atcaatttga
     2101 ttgtggaaaa aggagaattt gtagctgtca tggggcgtag tggtagtgga aaaacaacac
     2161 ttttaaatgt tacttcaatt attgataaaa ttgatagtgg gagtatttat tgtgcagaca
     2221 aagaaattag tgcattttct gataacgagg caactagttt tagaaaaaat gatattggtt
     2281 ttgtttttca agattatatg ctgttagaca gtctaaccat tagggagaat atttctgttg
     2341 ctctatcttt aaaaaatgtt gattcatcaa aaattgatga tttgataaat aactatgcaa
     2401 aaagatttaa tttatatgag cagttgaaaa aatatccgta tcaactttct ggaggtcaaa
     2461 gacagagggt cagtattatc agagcaatca ttaaagaacc agagattatt tttgctgacg
     2521 aaccaactgg agcgcttgac ttaaaatctt ctgaaggaac aatgaggatt ttgagtgaaa
     2581 tcaataaaat agaaaaggta actattttaa tggttactca tgacgtttta tcagcttctt
     2641 atgcggatag agttgtactt ctcaaagatg gtaaacttca catggagatt gataaagagg
     2701 attgtggaga gacgttttat gatgtaatta gtcaagcctt atccgataga ggagagtaat
     2761 atgatttggt ccattacaaa atctaacatc aaaaaaaact tttcgttata tcgtatctat
     2821 ttt
//
LOCUS       DATUYR010000105         2800 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.105, whole genome shotgun sequence.
ACCESSION   DATUYR010000105 DATUYR010000000
VERSION     DATUYR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.105"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            123..821
                     /gene="tsaB"
                     /locus_tag="VCI33_001638"
     CDS             123..821
                     /gene="tsaB"
                     /locus_tag="VCI33_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEP2832539.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHAPLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            793..1248
                     /gene="rimI"
                     /locus_tag="VCI33_001639"
     CDS             793..1248
                     /gene="rimI"
                     /locus_tag="VCI33_001639"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEP2832540.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKFIGKRPDYYRNPIETALLMKREGKHDR"
     gene            1238..2266
                     /gene="tsaD"
                     /locus_tag="VCI33_001640"
     CDS             1238..2266
                     /gene="tsaD"
                     /locus_tag="VCI33_001640"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEP2832541.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGNELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(2437..2706)
                     /gene="rpsN"
                     /locus_tag="VCI33_001641"
     CDS             complement(2437..2706)
                     /gene="rpsN"
                     /locus_tag="VCI33_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEP2832542.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
BASE COUNT          845 a          485 c          609 g          861 t
ORIGIN      
        1 ttagtaaaaa atttaggtac atttatggta tagtctaaat caaattaaga gtcagccttt
       61 ttgtttatga ttcttaattt gtgttataat tgatactaga acttgcagta gaaaggcata
      121 aaatgaagac acttgcattt gatacctcaa ataaaacctt gtcccttgct atacttgatg
      181 atgagacact tctagcagat atgaccctta acattcagaa aaatcatagt gttagcctta
      241 tgcctgctat tgattttttg atgacttgta ctgatcttaa acctcaagat ttagaaagaa
      301 tagtggttgc aaaaggccct ggatcttaca caggtttacg agtggcagtt gctactgcaa
      361 aaacgttagc gtacagttta aatattgcat tggtcgggat ttccagtcta tatgctttgg
      421 ctgcgtctac ttgtaaacag tatccaaata ctttggtggt gccattgatt gatgctagaa
      481 ggcaaaatgc gtatgtaggt tattatcggc aaggaaaatc agtgatgcca caagcccatg
      541 ctccactaga agttattata gaacaattag tagaagaagg gcagctgatt tttgttgggg
      601 agactgctcc ttttgctgag caaattcaaa agaaactacc tcaggcgata ctacttccaa
      661 ccctgccttc tgcttacgaa tgtggtcttt tggggcaaag tttggcacca gaaaatgtag
      721 acgcctttgt ccctcaatat ctcaagagag tggaagctga agaaaactgg ctcaaagata
      781 atgagataaa agatgatagt cactacgtta agcgaatcta atatgaaaac agttgaagaa
      841 caagcaaaaa atatttatca gcttttggaa atggtttatg gcacgtctcc ttggacttta
      901 gaacaagtat taatcgatat acggcgggat caaacagatt atttcctact atatgatcat
      961 gataaattgc taggtttttt agctattcaa gatttagctg gtgaagttga aatgactcaa
     1021 attgctatac tacctagtca tcaaggactt gggctagcgt ctcaattgat gacgcattta
     1081 gatagcattg aaagtgacat ctttttagag gttagagaat ctaatcatag agcacagggt
     1141 ttatatcaga agtttggttt taaatttatt ggcaaaagac cagactacta tagaaatcct
     1201 atagaaacag ctttattgat gaaacgggaa ggaaaacatg acagataggt atattttagc
     1261 agttgagagt tcttgtgatg agaccagtgt agcgatttta aaaaacgaaa gtaccctctt
     1321 aagtaatgtc attgcaagtc aggttgagag ccacaaacgc tttggtggtg tggtgccaga
     1381 agtagctagt agacaccatg tcgaagtgat tacgacttgc tttgaggatg ctttgcaaga
     1441 agctggcatc agtgcttcag atttatcagc agttgcggta acttatggcc ctggacttgt
     1501 tggagccttg ttagttggat tagcagctgc gaaggctttt gcttgggcaa atcatttacc
     1561 tttgattccg gttaatcata tggcaggtca cttaatggca gcgcgtgaac aaaagccttt
     1621 agtttatcct ttaatagcac tattggtttc aggaggacac acagaattgg tttatgtccc
     1681 agagccaggg gattatcata ttattggcga gacgcgtgac gacgctgttg gtgaggctta
     1741 tgataaagtt gggcgtgtga tgggcttaac ctatccggca ggacgtgaaa ttgatcagtt
     1801 ggctcataaa ggtcaagata cttatcattt ccctcgtgct atgataacag aagaccactt
     1861 agaattttct ttttcaggac tcaagtccgc ttttatcaat cttcatcata atgctaagca
     1921 aaaaggcaat gaactgattc tagaagattt gtgtgcatct tttcaagcag ctgttctaga
     1981 tattttattg gccaaaacaa aaaaagctct gagcagatac cctgcaaaga tgcttgtagt
     2041 ggctggtggt gtagcggcaa atcaaggatt acgagataga ttagcacaag aaattacaca
     2101 tatagaagtt gtgattccta aactaagact ctgtggagat aacgcgggta tgattgcttt
     2161 agcagcagct attgagtatg acaaacagca ttttgctaat atgagcctaa acgcgaagcc
     2221 tagtttagct tttgaccagt ttccagacag ttttgtaata aactagtcac acttgctagt
     2281 tagtaagatt tataatggat aagaatctcc ctttgaaaaa acaacccggt atcttggcat
     2341 accgggttgt catgatgata agtaattggc ttctatggtt actaataatt cagataatta
     2401 cctgcaaaat aattgtctgc ctaaggtgaa gtaagcttac cagctagctt tagtgacgcc
     2461 aggaagctgt cctttgtgag ctaagtcacg gaaattgata cggctaacac caaacttacg
     2521 catgtaagca tgaggacggc cgtcaatttt gtcgcggttc ttcaagcggt ttggatttga
     2581 atcacgaggc aatttgcgca gtgattcata atcgcccttc gcttttaagt cacgacgaag
     2641 gtcagcgtac tgttcaatca gttggagctg cttttggtat ttagcaattt ttgacttttt
     2701 tgccattgct ttctccttct taatatttaa taacctcaga gtcgttatca gcgctctgaa
     2761 tccacttaac tggtatttaa ctggttaagt ggatatataa
//
LOCUS       DATUYR010000106         2681 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.106, whole genome shotgun sequence.
ACCESSION   DATUYR010000106 DATUYR010000000
VERSION     DATUYR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.106"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            100..333
                     /locus_tag="VCI33_001642"
                     /pseudo
     CDS             100..333
                     /locus_tag="VCI33_001642"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            361..588
                     /locus_tag="VCI33_001643"
                     /pseudo
     CDS             361..588
                     /locus_tag="VCI33_001643"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            715..870
                     /locus_tag="VCI33_001644"
                     /pseudo
     CDS             715..870
                     /locus_tag="VCI33_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(917..1447)
                     /locus_tag="VCI33_001645"
     CDS             complement(917..1447)
                     /locus_tag="VCI33_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEP2832543.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(1440..>2681)
                     /locus_tag="VCI33_001646"
     CDS             complement(1440..>2681)
                     /locus_tag="VCI33_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HEP2832544.1"
                     /translation="SGGKDSGLLLNILLDFRDKYYPDREIGVFHQDFEAQYSLTTKYV
                     QETFTSLEGRKKVSLYWVCLPMATRTALSSYEMFWYPWDDKTEDIWVRPMPSQDYVIN
                     LENNSITTYRYKMNQEDLAKQFGRWYKQIHGNQKTVCILGNRASESLHRYSGFINKKY
                     GYQKECWITKQFKDVWTASPLYDWSVEDIWHAYYKFSYSYNELYDLFYKAGLKPSQMR
                     VASPFQDYAVDSLNLYRIIDQETWVKLLGRVQGVNFSNIYGRTKAMGYKSIALPKGHS
                     WKSYTQFLLSTLPVRLRNNYVRKFNKSIDFWHKTGGGLAEETINELIEKGYRIARNGI
                     SNYTSFKHSRVIFLDQIPDDTDDIVTTKDIPSWKRMCFCILKNDHICRTMGFGLTREQ
                     QKIVAFLKEKYKTSLKELESE"
BASE COUNT          870 a          544 c          381 g          886 t
ORIGIN      
        1 ctctatgcta aaggaagtcg atatcaatat aaatactata accgtcgacg ttcttcatga
       61 actcgtccca atcttgttaa tcaatgtttc caagcgactg ataagaataa attatggcta
      121 ggagacttaa cctacattcc tacacaagaa ggaatacttt atgtgtctgt cttgatagac
      181 gtttatagta gaaagattgt tggttgggca atgggccgac gtatgcaaga caagttggta
      241 acagaagctt tcaatcaagc ttataataga gaaaagccta aagaaggggt aatcgtccat
      301 acagatcagg gttctcaata taccggagca tgatttcagg acctacttag acagaaaaaa
      361 tgtaagtcca ggatgagtcg aaaaggtaat ccatacgata atgcactcat ggaatccttt
      421 tataaaacat taaaaagaga gcttgtcaat gatgctcatt tcgcaactat tgagcaagct
      481 cagcttgaga tttttaaata tagtgagact tactataacc ctaaacgtct gcattcagct
      541 ttagggtatc tctcacctgt agaatttgaa aaaatagtta ctcattagct taactttatg
      601 tctatttttt cttgacaagt ccagggtatg aagtcccctt atacacatac tttcaaaaca
      661 gctctcaatc ctctgtccgc tacaaaaata cgtcaccgct gccctccagc tgcctcattc
      721 aacgacaaac ttgaagccac aaacagactc atcaagggca ttaagtacaa tgcctttaga
      781 tttcagaact ttgacaactt taaaaccaaa atcctcatcg ctttgaacat ccaaaaagag
      841 agaaccaatc tgattctctc tcgttgttaa tgataagtca cccactacag ttgacaatga
      901 gtctcttact tcctgtttac tcatttaata gctcttctgg ttcccaagat tgattaaact
      961 catggtcctt aaaaagagaa gccaatcctg ttatttgttt cagtcttaaa atttcatctt
     1021 tatccattcc taaatgtttt gcaatccagt tatcacttcg cccacactca tgaagatcct
     1081 ttacaatctg actcatcaaa tcaacatcat gacttcctct agctctatta tgtcgaatgg
     1141 tagaagccat tcgatagtct aatgacttat caataacaga aacaggtaat ctcccacttt
     1201 ccctttcata aatatctgaa taatctagca ttatacgata tctatgaaaa ccatcaacaa
     1261 tactataccg atcctcttcc ttatcgtaat agcaaacaat cggcatagta taaccatcag
     1321 attttatact atcatacaat aatttcattt caggcggagc aactgaattc ggattataat
     1381 catttggaga aatcttttca attggaattg atttaatctc atagactgga ctaataaaat
     1441 tactcacttt ctaactcctt taaactcgtc ttatatttct cttttaaaaa ggctactatt
     1501 ttttgttgct ctcttgtcaa accaaacccc atagttcgac aaatatgatc gttttttaaa
     1561 atacagaaac acattcgttt ccagcttggt atatcctttg ttgttacaat atcgtctgta
     1621 tcatcaggaa tttgatctaa aaaaataacc cttgaatgtt taaaagaggt ataatttgaa
     1681 ataccatttc tagcaatacg atatcctttt tcaattagtt cattaattgt ttcctccgca
     1741 agtcctccgc ctgttttatg ccaaaagtct attgatttat taaattttct aacataatta
     1801 tttcttaacc tcactggtag cgttgataaa agaaactgag tgtacgactt ccacgaatga
     1861 ccctttggta aagcaattga tttatatccc attgctttag tacgtccata aatattcgaa
     1921 aagttaactc cttgaacacg tcccaaaagc tttacccaag tttcttgatc tattattcta
     1981 taaagattta ggctatcaac agcatagtct tgaaaaggag atgcaactct catttgtgat
     2041 ggttttaaac ccgctttata gaatagatca taaagttcgt tataagaata agaaaactta
     2101 tagtaagcat gccatatgtc ttccactgac caatcatata agggagatgc tgtccagaca
     2161 tctttgaatt gtttggtaat ccagcattcc ttttgataac catacttttt attgataaaa
     2221 ccactatatc tgtgtaagga ttctgaagca cggtttccta atatacaaac tgttttttga
     2281 tttccgtgaa tttgtttata ccacctacca aattgtttcg ctaaatcctc ctgattcatt
     2341 ttataacgat aagtagtaat tgaattattt tccaaattaa taacataatc ttgagatggc
     2401 attggtctta cccagatatc ttctgttttg tcatcccacg gataccaaaa catttcataa
     2461 ctactaagtg cagttctagt agccatcggt aagcacaccc aatacaaaga aacttttttt
     2521 ctaccttcta aagaagtgaa tgtttcttga acatattttg ttgttaaact gtactgtgct
     2581 tcaaaatctt gatgaaacac acctatttct ctatccggat aatatttatc tctaaaatcc
     2641 agtaaaatat taagaagtaa cccactatct tttccacctg a
//
LOCUS       DATUYR010000107         2467 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.107, whole genome shotgun sequence.
ACCESSION   DATUYR010000107 DATUYR010000000
VERSION     DATUYR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.107"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            15..428
                     /gene="yaaA"
                     /locus_tag="VCI33_001647"
     CDS             15..428
                     /gene="yaaA"
                     /locus_tag="VCI33_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEP2832545.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRIGDKISLPDQDLIITIVEPSQEEKEQFAEEIAEKTRVAALVKQMNQANKK
                     TSSKHKNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            430..1536
                     /gene="recF"
                     /locus_tag="VCI33_001648"
     CDS             430..1536
                     /gene="recF"
                     /locus_tag="VCI33_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEP2832546.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDNLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQVDSDIH"
     gene            complement(1593..2456)
                     /locus_tag="VCI33_001649"
     CDS             complement(1593..2456)
                     /locus_tag="VCI33_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEP2832547.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIIGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
BASE COUNT          871 a          488 c          415 g          693 t
ORIGIN      
        1 aaatggagat gacaatgata tacaaattat ttacagaatt tatcacccta caagcccttc
       61 ttaaagaatt aggaattatc caaagtggtg gcgctatcaa gggatttctc gctgaaacaa
      121 ccgttctatt taatggtgaa gatgagaaac gtcgaggcaa aaaaatcaga ataggggata
      181 agatttctct acctgaccaa gacttaatta tcactattgt tgagcctagt caagaagaaa
      241 aagaacaatt tgccgaagaa atagctgaaa agacacgtgt agcggctcta gtgaaacaaa
      301 tgaaccaagc taacaaaaag acttcttcta aacataaaaa tcgtcaatct actaccaaaa
      361 aatcactaag agcaactaag aaaactaaag gtaaaccaac tgcacctgtc cgcttcccag
      421 gtatctagta tgtggattaa agagttagag ttaaaacatt atcgcaatta tgaccacctg
      481 cttgcctcat tttcgtcggg tttaaatgtc tttatcggta ataatgctca aggaaaaacc
      541 aattttctcg aggcaatcta ttttctatca cttactagaa gtcatcgcac gagggctgat
      601 aaagaattga ttcattttga tcactcaact gtttctctta ctggtaagat tcagcgtatt
      661 agtggtactg ttgatttaga aatcaatcta tcagataaag gacgtgtgac aaagattaat
      721 gcgctaaagc aagcaaaatt atctgattat attggaacga tgatggttgt gctttttgca
      781 ccagaagatt tgcaactagt taagggagca cctagccttc gtcgcaaatt tatcgacatt
      841 gaccttggtc aaattaagcc tgtttactta tctgagttat cccattataa ccacgtgctt
      901 aaacagcgta atagctattt aaaaagtgcc caacaaattg atgccgcttt tctagctgtt
      961 ctagatgagc aattagctgg ctatggagcc cgtgtcatgg aacatcgtat tgattttatc
     1021 aatgcgttag agaaagaagc aaatactcat caccaagcca tctcaaatgg tttagaaagt
     1081 ctctctctct cctatcagtc gtcagttgtc tttgacaaaa aaactaatat ttaccaacaa
     1141 tttttacacc aacttgaaaa aaatcatcag aaagatttct tccgtaaaaa cactagcgtt
     1201 ggtccacatc gcgataactt agccttttac attaacggga tgaatgctaa ctttgctagt
     1261 caaggtcagc accgcagcct tattctttcc ttaaaaatgg ctgaagttag tttaatgaag
     1321 gccttgactg gagataaccc cattctttta ctagacgatg tcatgagcga gctagacaat
     1381 acacgacaaa caaagttact tgaaacagta attaaagaaa atgtccaaac ctttatcaca
     1441 acaacaagtt tggatcacct gtcacagttg ccagaaggta tccgtatttt tcacgtaaca
     1501 aaaggaaccg ttcaagttga ttctgatatt cactaataac atcaaaaaaa gggttcagtt
     1561 aaactgagcc tttttttgat attatgtggc gattaagatt tgaccacacc taataaaata
     1621 gcacctacga taaaacaaat aattcctgtg acaacccaac gcatttcttt tttggttttg
     1681 gtttcgccta ggaataaaat gcctccaaca atcgaaatga tagcacctaa ttgtgagaaa
     1741 ctaaaggcaa tagcaagtcc tgcttttgat gcagctaaaa gcatgaaaat attcccaatg
     1801 ccccaaagta atccaacaac gctattttta atcacatact ggtcaatgga aattttaaat
     1861 gacatgaaag ttatagctcc taaaaccata cctactgcca tgggcaaaat gactgacaaa
     1921 acttcaaatt tcatgatgtt attaaataat acagcataca tcacgtaacc aattgttgaa
     1981 taagtgagcg ccctaaatcc ttttgaaaaa ttatgaaggt gattgacttg tgcattagca
     2041 tcatcttgtt tactagaaaa gtaaaaacca ataatcagaa gtaatagtgc tagacttcct
     2101 actacaaatt gcataggtct tgtccattca tgaaaaacga ggacaccaat caaacttccc
     2161 agaacaagtt gagagccact tgacaaagga ttggcaacgg agactcccat gtattgcatg
     2221 gcatgaaatt gaccagtttg accaattgac cagataaaac cacccaatat tccaaaaagc
     2281 caaagttgca gtgtcatctc aggtctaaca attaaccaaa cagctaatga aaataataaa
     2341 gccccaaaag tcattcctag agtttgttgc gaaggttttc ctccaatttt gttactaaca
     2401 aatccaatgc ttccccatgt aaacatggga ataagtgcat aaaaaatacc ttccaaaatg
     2461 ttatctc
//
LOCUS       DATUYR010000108         2381 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.108, whole genome shotgun sequence.
ACCESSION   DATUYR010000108 DATUYR010000000
VERSION     DATUYR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.108"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..247
                     /locus_tag="VCI33_001650"
     CDS             <1..247
                     /locus_tag="VCI33_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEP2832548.1"
                     /translation="MFLFILPTYNLVKSYVDFEKQNQQVVKLKKEYNELSKRTKKEKQ
                     LAERLKDDNFVKKYARAKYYLSREGEMIYPIPGLLPK"
     gene            382..1668
                     /locus_tag="VCI33_001651"
     CDS             382..1668
                     /locus_tag="VCI33_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEP2832549.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITAIADDVYGILK"
     gene            1665..>2381
                     /gene="tilS"
                     /locus_tag="VCI33_001652"
     CDS             1665..>2381
                     /gene="tilS"
                     /locus_tag="VCI33_001652"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEP2832550.1"
                     /translation="MMTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPF
                     LTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLALENSLL
                     FQAFKELTNHITTTDLTEFNE"
BASE COUNT          891 a          349 c          379 g          762 t
ORIGIN      
        1 gatgttttta tttattttgc caacttataa tcttgtcaaa agttatgttg attttgaaaa
       61 gcaaaatcaa caggtggtta aattaaaaaa agagtataat gaattgtcaa agaggacaaa
      121 aaaagaaaaa caattagcag aacgactaaa agatgataat tttgtcaaaa aatatgctag
      181 ggcaaaatac tatttatcgc gtgaaggaga aatgatttat cctattccag gactattacc
      241 aaaatgatga tggataatat tgtaaaaaaa atagaagcat ttcttgcttt ttctgataaa
      301 aaattagcag agctgcaaca ggaaaatcaa aaagttaaag aagaaagtca gtatattcaa
      361 aaaaactaga aaggagtcaa tatgagaaaa ttattagcgg ctatgttaat gacttttttt
      421 ctgactcctt taccagtgat tagtacagaa aaaaaactta tattttcaaa aaatgctgtt
      481 tatcaattga aacaagatgt cgttcaatca acacaattct ataatcagct accctctaat
      541 ccaaatcttt atcaagaaac gtgtgcctat aaagacagtg atttaactct accagcagga
      601 agattaggtg taaatcaacc attacttatt aaatcgcttg ttcttaacaa agaatcttta
      661 ccggtttttg agttagctga tggtacctat gttgaggcta atcgacaatt gatttatgac
      721 gatattgtac ttaatcaagt agatatagat agctattttt ggacacaaaa gaaacttagg
      781 ctttattcag ccccttatgt tttaggtacg caaacaattc cttcttcttt ttcatttgct
      841 caaaaagttc atgccactca aatggcacaa acaaaccatg gaacttatta tcttattgat
      901 gataagggct gggcatcaca agaagatcta gttcaatttg ataaccgcat gttaaaagtc
      961 caggaaatgc tcttacaaaa atataataac ccaaattatt caatttttgt aaagcaactc
     1021 aacacacaaa caagtgctgg tattaatgct gataaaaaaa tgtatgctgc aagtatctcg
     1081 aagttagcac cactttatat tgttcaaaaa caattacaaa aaaagaaatt agcagagaat
     1141 aaaactttga cttatactaa agatgttaat catttttatg gagactatga tccattggga
     1201 agtggtaaaa ttagtaaaat agctgataat aaagattatc gtgttgaaga cctactgaaa
     1261 gctgtagcac aacaatcgga taatgtagca actaatattt taggttatta tctatgtcat
     1321 cagtatgata aagctttccg ctcagagata aaagctttat caggtatcga ttgggatatg
     1381 gagcagcgct tattaacttc tcgttcagct gcaaatatga tggaagctat ttatcatcaa
     1441 aaaggccaaa ttatttctta cctttcaaat accgaatttg atcaacaacg tatcacaaaa
     1501 aatattactg ttccagttgc acataaaatt ggtgatgctt atgattataa acatgacgtt
     1561 gctattgttt acggtaatac tccatttatt ttgtctattt ttacaaataa atcaacctat
     1621 gaagatatta cggctattgc agatgacgtt tatggtattt taaaatgatg acctatcaag
     1681 aaatttttaa tgaaataaaa aataaagcct actttaaaaa tcatagacat gtgttaatag
     1741 ccgtatctgg tggagtagat tcaatgaatt tattgcactt tttatacctg tttcaagaca
     1801 aattgaaaat tcgtattggt attgctcatg ttaatcacaa acaaagatca gaatcagatt
     1861 cagaagaagc ttatttaaag tgttgggcta aaaaacatga catacctatt tatgtttcta
     1921 attttgaagg aatattttca gaaaaagcag cacgtgattg gcgctatgct ttttttaaaa
     1981 gtattatgtt aaaaaataac tattcagctt tagtaacagc tcaccattca gatgatcaag
     2041 cagaaactat tttgatgagg ctaattagag gaagccgatt gaggcacttg tcgggtataa
     2101 aaagcgtgca accatttgcc aatggtcaac ttataagacc ttttttaact ttctcaaaaa
     2161 aggacttgcc agaaattttt cattttgaag attcttcaaa tagggaatta tcatttttaa
     2221 gaaatagagt tagaaataat tatttaccat tgttaaagca ggaaaatcct aggttcattc
     2281 agggattaaa tcaattagca cttgaaaata gcttactttt tcaagctttt aaggagctaa
     2341 caaatcatat tactaccact gatttaacag aatttaatga g
//
LOCUS       DATUYR010000109         2291 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.109, whole genome shotgun sequence.
ACCESSION   DATUYR010000109 DATUYR010000000
VERSION     DATUYR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.109"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            1..1116
                     /gene="hasA"
                     /locus_tag="VCI33_001653"
                     /pseudo
     CDS             1..1116
                     /gene="hasA"
                     /locus_tag="VCI33_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
     gene            1152..>2291
                     /gene="hasB"
                     /locus_tag="VCI33_001654"
     CDS             1152..>2291
                     /gene="hasB"
                     /locus_tag="VCI33_001654"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HEP2832551.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEDYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFDNTYLALRVAYF
                     NELDTYAESRKLNSHMIIQGISHDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPMLNKLESEDQSVLVNDLENFKKQANIIVTN"
BASE COUNT          791 a          365 c          385 g          750 t
ORIGIN      
        1 gattatcttt cctttatgag ccatttaaag gaaatccaca tgactataaa gttgctgccg
       61 taattccttc ttaataatga agatgccgag tcattattag aaactcttaa aagtgtgtta
      121 gcacagacct atccgttatc agaaatttat attgttgatg atgggagttc aaacacagat
      181 gcaatacaat taattgaaga gtatgtaaat agagaagtgg atatttgtcg aaacgttatc
      241 gttcaccgtt cccttgtcaa taaaggaaaa cgccatgctc aagcgtgggc atttgaaaga
      301 tctgacgctg acgttttttt aaccgtagat tcagatactt atatctatcc aaatgcctta
      361 gaagaactcc taaaaagctt caatgataag acagtttatg ctgcaacagg acatttgaat
      421 gctagaaaca gacaaactaa tctattaacg cgacttacag atatccgtta cgataatgcc
      481 tttggggtgg agcgtgctgc tcaatcatta acaggtaata ttttagtttg ctcaggacca
      541 ttgagtattt atcgacgtga agtgattatt cctaacttag agcgctataa aaatcaaaca
      601 ttcctaggtt tacctgttag cattggggat gatcgatgtt taacaaatta tgctattgat
      661 ttaggacgca ctgtctacca atcaacagct agatgtgata ctgatgtacc tttccaatta
      721 aaaagttatt taaagcaaca aaatcgatgg aataaatctt tttttagaga atctattatt
      781 tctgttaaaa aaattctttc taatcccatc gttgccttat ggactatttt cgaagtcgtt
      841 atgtttatga tgttgattgt cgcaattggg aatcttttgt ttaatcaagc tattcaatta
      901 gaccttatta aactttttgc ctttttatcc atcatcttta tcgttgcttt atgtcgtaat
      961 gttcattata tggtcaaaca tcctgctagt tttttgttat ctcctctgta tggaatatta
     1021 cacttgtttg tcttacagcc cctaaaactt tattctttat gcaccattaa aaatacggaa
     1081 tggggaacac gtaaaaaggt cactattttt aaataatata tgcatcgagt agttagagaa
     1141 ggagtaattt tatgaaaata gcagttgctg gatcaggata tgttggatta tcactaggag
     1201 ttcttttatc acttcaaaac gaagtcacta ttgttgatat tcttccttct aaagttgata
     1261 agattaataa tggcttatca ccaattcaag atgaatatat tgaagattac ttaaaaagta
     1321 agcaattatc tattaaagca actttagata gcaaagcagc ttataaagaa gcggaactgg
     1381 tcattattgc cacacctaca aattacaaca gtagaattaa ttattttgat acacagcatg
     1441 ttgaaacagt tatcaaagag gtactaagcg ttaatagcca tgcaactctt atcatcaaat
     1501 caacaattcc aataggtttc attactgaaa tgagacagaa attccaaact gatcgtatta
     1561 tcttcagccc tgaattttta agagagtcta aagctttata tgacaactta tatccaagcc
     1621 gaattattgt ttcttgtgaa gaaaacgatt ctccaaaagt aaaggcagac gcagaaaaat
     1681 ttgcactttt attaaagtct gcagctaaaa aaaataatgt accagtactt attatgggag
     1741 cttcagaagc tgaagcagta aaactatttg acaatactta tttagcgtta agggtagctt
     1801 attttaatga gttagacact tacgcagaat cgagaaaatt aaatagtcac atgattattc
     1861 aaggaatttc tcatgatgat cgaataggaa tgcattataa taacccatca tttggttatg
     1921 gaggttattg tctacctaaa gatacgaagc aattattggc aaattacaat aatattcccc
     1981 aaacgctaat tgaagctatc gtttcatcaa ataatgtgcg caagtcctat attgctaagc
     2041 aaattatcaa cgtcttagaa gagcgggagt ccccagtaaa agtagtcggg gtttaccgtt
     2101 taattatgaa aagtaactca gataatttta gagaaagtgc tatcaaagat gttattgaca
     2161 ttcttaaaag taaagacatt aagataatta tttatgagcc aatgttaaac aaacttgaat
     2221 ctgaagatca atctgtactt gtaaatgatt tagagaattt caagaaacaa gcaaatatta
     2281 tcgtaactaa t
//
LOCUS       DATUYR010000110         2192 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.110, whole genome shotgun sequence.
ACCESSION   DATUYR010000110 DATUYR010000000
VERSION     DATUYR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.110"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(268..>2192)
                     /gene="ftsH"
                     /locus_tag="VCI33_001655"
     CDS             complement(268..>2192)
                     /gene="ftsH"
                     /locus_tag="VCI33_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEP2832552.1"
                     /translation="IIVVITGFQFYLKGTSTQSQQISYSKLIKHLKAGDIKSLSYQPS
                     GSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSLVLPSDTILKEMTAAADKNG
                     TELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGGGGARGAMSFGKNKAKSQSK
                     GDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGARIPAGVLLEGPPGTGKTLLA
                     KAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAERAIIFIDEIDAVGRRR
                     GAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNRSDVLDPALLRPGRFDRKVL
                     VGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFVGADLENVLNEAALVAARRN
                     KIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHEAGHTIVGLVLSNARVVHKV
                     TIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGRVAEEIVFNAQTSGASNDFE
                     QATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEKSYSAQTAQMIDDEVRELLN
                     QARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIYETGKMPVDLETDDNEAHAL
                     SYDEIKNKMTESESAE"
BASE COUNT          656 a          476 c          335 g          725 t
ORIGIN      
        1 gtaaatttca tcttattggt tgactgaaac ttttgctatc ttctgttctt ctattattct
       61 aagaaaacac taatagattt tttcaaatgg ctttaacaat tttctagttt taaataccaa
      121 agtctttctc actttaagta ataaagaaaa aaagtatgtc atcttctcat ttctagcaat
      181 taggcaccta ttacaattca taacgtttac aaggatattg tgttgataaa aaagctgaac
      241 ttaatctata agttcagctt ttttatatca ttctgcagat tcagattctg tcattttatt
      301 tttaatttcg tcataagata acgcatgagc ttcattatca tcagtttcta aatcaactgg
      361 cattttacca gtttcataaa ttgatttaat ttgtgcagcg tctagagttt catatttgag
      421 aagagcttca gcaatcaatt tatgagtatc tcgattctca ttgatgatat cagctgcttg
      481 attacgagct tgatttaaga gttcacgaac ctcatcatca atcatttgag ctgtttgggc
      541 agagtaagat ttttcaggag agatctgtcc tggcatcatc gcatggttac cttcatattg
      601 aactggtcca agcttttcac tcataccata ttccgtaacc atggcccttg ctatctgagt
      661 agcttgctca aagtcatttg acgcacctga tgtttgagca ttaaaaacaa tttcttcagc
      721 aacacgacca cccatcaatc ctgctaattg ctctttcaga tcttctttag aaagaagcat
      781 ttgatcttct tttggaagag cgatcatata accacctgct cgtccacgag gtacaatagt
      841 taccttgtga actacacgcg cattagataa aactaaacca acaatagtat gaccagcttc
      901 gtgataagca accatttctc gctctttttg cgaaatagtg cgatctttct ttgatggtcc
      961 agcaataaca cgatcttcag cttcatcaat atcactagcg tcaattttaa ttttatttct
     1021 gcgagcagca accaacgcag cttcatttaa aacattttca agatcagcac ctacaaaacc
     1081 tggagtctgt tgggctacta ctttcaaatt aacgtcattt gcaagtggtt tatttttagc
     1141 atgaacacgt aaaatggctt cacgtccttt aacatcaggg cgaccaacta aaaccttacg
     1201 atcaaaacga cctggtctta gtaaagctgg atctaaaaca tcactacggt tagttgcagc
     1261 aataacgata atgttctcat taccctcaaa accatccatt tcaatgagta attggttaag
     1321 ggtctgttct cgttcatcgt taccgcctcc catgccagca ccacgacgac gacctacagc
     1381 gtcaatttca tcgataaaga tgattgcacg ctcagctttt ttagcatcct caaataaaga
     1441 acgaacacga ctagcaccta caccgacaaa catttcgaca aaatctgaac ctgaaatact
     1501 gaaaaatggc actccagctt cacctgctac agctttagct agtaatgttt taccagtacc
     1561 tggaggcccc tctagaagaa ctcccgctgg gatccgtgct cctagtgatt tatatttttt
     1621 aggatttttc aaaaaatcaa caacttctac taattcttgt ttttcttctt cggcaccagc
     1681 aacgtctgta aatctcactt taacatcgcc ttttgattga gattttgcct tatttttgcc
     1741 gaaactcata gcaccacgag ctcctccacc accttgattc atcatcatca tcatgaaagc
     1801 agcaaaaata acaataggta aaaagctcat taagaaggta atccaagtac cacttgaact
     1861 ttcctgctta actgtaagct ctgttccatt tttatcagct gctgctgtca tttctttcaa
     1921 gatggtatca ctaggtaata ctaaagatga gaactctgtt acctgtgttg aagctcttcc
     1981 acctaaaaat gatagtcctg aattaactgt cactttttga ggtttctcat atttcccttt
     2041 aacttcaata atactaccgc taggctgata acttaaagat tttatatctc cagccttaag
     2101 atgtttaatt aatttggaat aactaatctg ttggctttgt gtgctggttc ccttaagata
     2161 aaattgaaat cctgtgatga caacaataat ca
//
LOCUS       DATUYR010000111         2134 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.111, whole genome shotgun sequence.
ACCESSION   DATUYR010000111 DATUYR010000000
VERSION     DATUYR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.111"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            109..753
                     /locus_tag="VCI33_001656"
     CDS             109..753
                     /locus_tag="VCI33_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEP2832553.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
     gene            763..1458
                     /locus_tag="VCI33_001657"
     CDS             763..1458
                     /locus_tag="VCI33_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEP2832554.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTIAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(1460..2131)
                     /gene="radC"
                     /locus_tag="VCI33_001658"
     CDS             complement(1460..2131)
                     /gene="radC"
                     /locus_tag="VCI33_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEP2832555.1"
                     /translation="MKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHVLEL
                     SSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTDSVL
                     TSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIFEEKTIFIGTVRRSLAEPREILYYAC
                     KNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYSFRE
                     KSTLF"
BASE COUNT          667 a          402 c          368 g          697 t
ORIGIN      
        1 tagctttttt aactcataca aaaacaatca aaatgttgca tttttcacaa tctttttgtt
       61 acaataaaga cagacatatt taacgagaaa tctacaagga gtaaaatagt ggttattgac
      121 aaatctattc caaaagcgac tgcaaagcgc ttatcacttt attaccgtat ttttaaacga
      181 ttccatgctg accaagttga aaaagccagt tctaaacaaa ttgcagatgc aatgggaatt
      241 gactctgcta ctgtacgccg tgacttttct tattttggag aattggggcg tcgtgggttt
      301 ggctatgatg ttaccaaatt gatgaatttt tttgctgact tgcttaatga tcactctaca
      361 acaaacgtta tcttagttgg atgcggtaat atagggcgtg ctctattaca ttaccgcttc
      421 catgatcgta ataagatgca aattgccatg ggttttgata ccgatgataa cgctcttgtt
      481 ggtactaaaa cagctgataa tattccagtc catggtatct catccgtcaa agaacggatt
      541 gcaaacactg acattgaaac ggctatctta acggttccca gcattcatgc tcaagaagtt
      601 actgatcaac ttattgaagc aggaatcaaa ggaatcttga gttttgctcc tgttcaccta
      661 caagttccta aaggagttat tgttcaatct gtcgatttga ccagtgagct ccagacacta
      721 ctctatttta tgaatcaaaa tcatctagat taaggaggta taatgaccaa accgattatt
      781 ggaataactg caaatcaaag actaaatatg gctttggata atctcccatg gtcatatgca
      841 cctactggat ttgtacaggc agtcactcaa tctggggggc tgccgcttct tttacccatt
      901 ggtgatgaag ccgccgctaa aacttatgta tctatggttg ataaaatcat tttaattggt
      961 gggcaaaacg ttgacccaaa atattatcaa gaagagaagg ctgcttttga tgatgatttt
     1021 tcaccagagc gagacacatt tgagctagct atcatcaagg aggctataac attaaaaaaa
     1081 ccaatcttag ggatttgtcg tggtacacag ttaatgaatg ttgccttagg aggaaatctt
     1141 aaccaacaca ttgactcaca ttggcaagaa gcgccttctg atttcttatc acacgaaatg
     1201 attattgagc cagactctat cttatatcct atttacggtc ataagacatt aattaattct
     1261 tttcatcgcc aaagtttaaa aactattgct aaggatttaa aagtaatcgc tagagatcca
     1321 cgtgatggta ccatcgaagc agtcatatca accaacgatg ccattccttt tttaggtgtt
     1381 cagtggcatc ctgaacttct ccaaggtgtt cgagatgaag atctacaatt gttccgctta
     1441 ttcgtcaatg acttctgatt taaaaaaggg ttgatttttc tcgaaaacta taataatctt
     1501 tatagctaac gataatgtga tctagacaga taatgcctaa atcttcacat gatcgtttta
     1561 ttttttcagt aaagcaataa tcgttagaac taggttcaat atttcctgaa ggatgattat
     1621 gaataacaat gagactagtc gccatatttt tacaggcata gtataaaatt tctcttggtt
     1681 ctgcaagtga acgtcggaca gtcccaataa aaatagtttt ttcttcaaaa atacgatttt
     1741 gattatctaa atacaatacg actaaatgct cttgtttttt atctcctaaa gctgccatca
     1801 ttttttcagc gacctgaaca ctggttaata cgctatcagt taatgtctta tcggtcgcta
     1861 aaattcggga aaccaactca atcatagctt taatttcaat cgctttaact tttcctatac
     1921 ctgccaaatg ttgtaattct tgcaaagaca tctttttaaa atctgccaga ctgtctaaat
     1981 gcgataaaag ataggatgac agctctaaga catgcttttc tttattacct gttcgtaata
     2041 aaattgctaa taattcttga ttacttagag actctgctcc tagtcgcatc aaacgttctc
     2101 ttggcatggc tttattatca tcacatttta taga
//
LOCUS       DATUYR010000112         2130 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.112, whole genome shotgun sequence.
ACCESSION   DATUYR010000112 DATUYR010000000
VERSION     DATUYR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.112"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(1..676)
                     /locus_tag="VCI33_001659"
                     /pseudo
     CDS             complement(1..676)
                     /locus_tag="VCI33_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            995..1222
                     /locus_tag="VCI33_001660"
     CDS             995..1222
                     /locus_tag="VCI33_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEP2832556.1"
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            1235..1435
                     /locus_tag="VCI33_001661"
     CDS             1235..1435
                     /locus_tag="VCI33_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEP2832557.1"
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHP
                     VLAGGCVVGFTVGGAYLGYTAN"
BASE COUNT          674 a          323 c          440 g          693 t
ORIGIN      
        1 aaactatcaa gtgtgtcaaa gcatgctccg aatcctttat gaatacgagc tccgaactta
       61 atattatagt cctcgaaaat acttacaaga catcgttcta aggcttcttc atttgggaaa
      121 accactctct tcttgttttg tcgcttaatt tctttattga gtgattcaat caagtttgta
      181 gagtaaatgc tagaccaaat gtgatgtggg aattgataga aagtaaggag attttccgtc
      241 gtttcaagtg atgccatgac ctttttataa cgaggcttcc attcagaaac aagtggtgac
      301 catacatgcg ctcaataatg tcactgattt cgcgtgtcgt aacgccagtc tgatagagtt
      361 tgatcaccat ctcttccaag tggtcatctc gtctagcata agatggtaat aaaactggtg
      421 aaaactcgcc gtttcgatct cttgggatga ttaaattgac taagccatac tttgtttcaa
      481 actgacgaga ataagtccca ttacggctat tacctgtgtt gtaaccttct ttttcataag
      541 gttcataacc aagaaaagct gatagttcaa cttgcaagag gtcattcata gctatttcta
      601 aagaggagcg aaagaattca tcaatatctt gtttttgtgc taggaagtta agtaattctg
      661 tggtaaactg agtcatggga ataaatctct ttctagtgaa gtgtcgtaac tctactatac
      721 aagatttatt cctttttgtg tgtttacaca agatatttta cactaccatc aaaaatctta
      781 aaaggtattt ataaaagcat gaatggtatc atttaaactt cttaattaat taactattgt
      841 cacagcgtaa gtgtagagag gataaaaaga catttcatga tgaaaaatag ccttttaaga
      901 ttttgaaacc atgatactgt tctttatgat attctgtact agctcgaggt tgcaacgaga
      961 caaatttaag aattatgaaa agaagttatt cgatatggag ataaagaaac tggaaacatt
     1021 tcaccaaatg actatagaaa agcttgccaa ggttgagggt ggtaagaata attggcaggc
     1081 aaatgtcagt ggggttatcg ctgcaggtag tgctggagct gctataggtt ttccagtttg
     1141 cggtgtagcc tgtggttata ttggagctaa aacagctatt actctttggg ctggtgtaac
     1201 aggagctaca ggcggcttct agaggaaaga aaggatgaca acaatgaagg aattaacaat
     1261 taatgacatg gctagtataa gtggaggaaa cgctccagga gatgctgtaa ttggtggtct
     1321 tggaggctta gcttctggat tgaaattttg taaactgcca catccagtat tggcaggtgg
     1381 atgtgttgta ggatttacag ttggtggagc atatttggga tatacagcaa attaaatgat
     1441 ctatacgatt tttattatta tgaatgcgtc tataatgttt ttaatcattg ttgatgccat
     1501 tcataaaaaa atgttgataa atctatacca tatttagtgt ttttaatttt caatcaatta
     1561 tccctttcgg aaaaatcggt aaacatgtta tctatcgtag ctttggtagc gtcattattt
     1621 gtttttatcg ataaaagaaa atagtgatta tgccaatgaa tttagatatc actcttagct
     1681 ttttaaaata caaaagagtg aaattagaat gaaaaagact tcaaaaatat ggaagtgcta
     1741 aaaaaatggt ctgactatca taaatggagg cggaaaagac ggagcaaata tctttttgac
     1801 agggatggca ggtgctgcgc agggagtgac agtttgtgct cagacaggcg tatttatccc
     1861 ttggcaaggt tatattttgt gtggagcagc tggtgctgct acaaatatta tctggcctca
     1921 ctaaatatat aaatgactat ctatcactga tatataagta gtagaggagt ggtatgaaaa
     1981 ttttagatat tatttttaac ctatatgata gaaagatgat atttctagaa aatgttgaaa
     2041 agctgggagg ttacattgtc ccttttaccc ttatcatctt gagtgtagcc tacaacttta
     2101 ggcaccctta tcctatttgg gctgctgtac
//
LOCUS       DATUYR010000113         2120 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.113, whole genome shotgun sequence.
ACCESSION   DATUYR010000113 DATUYR010000000
VERSION     DATUYR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.113"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            74..553
                     /gene="rlmH"
                     /locus_tag="VCI33_001662"
     CDS             74..553
                     /gene="rlmH"
                     /locus_tag="VCI33_001662"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEP2832558.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(711..784)
                     /locus_tag="VCI33_001663"
     tRNA            complement(711..784)
                     /locus_tag="VCI33_001663"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Arg,seq:tct)
     gene            949..1723
                     /locus_tag="VCI33_001664"
                     /pseudo
     CDS             949..1723
                     /locus_tag="VCI33_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1918..1989
                     /locus_tag="VCI33_001665"
     tRNA            1918..1989
                     /locus_tag="VCI33_001665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1951..1953,aa:Glu,seq:ttc)
     gene            2023..2096
                     /locus_tag="VCI33_001666"
     tRNA            2023..2096
                     /locus_tag="VCI33_001666"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2057..2059,aa:Asn,seq:gtt)
BASE COUNT          719 a          374 c          398 g          629 t
ORIGIN      
        1 tattagtagt tgaagaaaaa taacttgtgg ataaattgtg taaaaagtaa tgttcttgtg
       61 atacaattaa gctatgaaag tgaaactaat ttgtgttgga aaactaaaag aaagatacct
      121 taaagatggt atttcagaat atcaaaaaag actttcccgt ttttgtcaat tcgaaatgat
      181 tgagttaact gatgaaagaa caccagataa agcaagtttt gctgataatc aattgattat
      241 gagtaaagaa gctcagcgaa tccataaaaa aattggggaa cgtgattttg taattgcatt
      301 agctattgaa ggaaaacagt ttccttcaga gacatttagt gaattaatat caggggttac
      361 tgttaaggga tattcaacta tcacgtttat tattggtggg agtcttggat tagattcaat
      421 tattaaaaaa agagctaata tgctaatgag ttttggattg ttaaccttac ctcatcagtt
      481 aatgcgtcta gttcttactg agcaaattta tcgtgctttt atgattacac aaggcagtcc
      541 ttatcataag taagagagtg tgaactggaa taactaactc ttaccacttt attagataat
      601 agttcggtat aattaaaagc aaatattaaa aaaagacgta ctaaatccta tcttttcaat
      661 acactaaggt aaatacaaaa aaaagcacta aaacagtgct tttttatctt tgatcccagc
      721 aggattcgaa cctgcgaccg ttcgcttaga aggcgaatgc tctatccagc tgagctatga
      781 gaccttaacc acatcatttt aacaaaaatt atctaaaact gtcaatattt attttgaaaa
      841 aactagaaaa taacataata tcccgcgttg taaaatgaag tgcaacaaaa aagacatgtt
      901 catctgatat actagaattc cccaattcag tatggaaagc agatgaacat gtctaatatt
      961 aattctacca gaaagtcatc ctattctcat ctttcagcca cagaacgggg tgaaattgct
     1021 gcttatctta aaatgggaaa gaaacccgtt gagattgctc gacttctggg gcgtcactgt
     1081 tctacaatct gccgagaaat aaaacgtggt tcagtagatc aggtaaagga taagaatgga
     1141 aaacaaacct tcttcaacgc ctatttcgct gatagtgggc aacgtgtcta ttaaaccaat
     1201 cgatttgcct aaggtggtta gtatcagaaa acgttctaag aaagtcatga agacgaataa
     1261 gaagacttta ggtaaatcta tcgaagaacg tccagaatat attaatgatc gttctgaatt
     1321 tgggcattgg gagattgatt tggttctcgg caagaaaacc aaaagtgaag ctgttatgtt
     1381 gactttggta gagcgccaga cacgctatgc actaggagtt aagctagaag acaagcagtc
     1441 ccaaaccatt aaccgtgctg tcacgcatct catcagtcag tatcctattg catccatcac
     1501 agcggataat ggttctgagt ttagtttact ctcagactta aaagctgttg acgtttactt
     1561 tgcacatccc tattcttcac atgagagagg aacaagcgag aacttcaatg gcctcctcag
     1621 agaatatgtc cctaaaggag tctcacttaa tccactaacc tctgaagaac ttgacaatga
     1681 tattactgcc atcaatgagc gcctaagacg acttcttcaa tagcaatcct caaaattcct
     1741 gtttgagcta tcccgaacag cttaacctct ggaactctag ttatctatga actcgttgca
     1801 cttgacttga caacttgcgg tttaaaaaaa atcaaatttt tttaaaaaaa gtatagacaa
     1861 aaaaaatcaa tctgctataa tagtaagagt gttaaggaga tatcctccaa aacttctggt
     1921 ccgttggtca aggggttaag acaccgcctt ttcacggcgg taacacgggt tcgaatcccg
     1981 tacggactat cgcaattgtc attttggcaa tttttttctt tttccggcat agctcagttg
     2041 gtagtagcgc atgactgtta atcatgatgt cgtaggttcg agtcctactg ccggagtgat
     2101 aagatatttc ctaagataat
//
LOCUS       DATUYR010000114         1938 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.114, whole genome shotgun sequence.
ACCESSION   DATUYR010000114 DATUYR010000000
VERSION     DATUYR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.114"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..249)
                     /locus_tag="VCI33_001667"
     CDS             complement(<1..249)
                     /locus_tag="VCI33_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEP2832559.1"
                     /translation="MIDFIISIDDCAVELDSRQSWKIGYPLSTILFLVFVCQLAGIET
                     WKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVISLVN"
     repeat_region   398..763
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=530..565
                     /rpt_unit_seq="gttttgggaccattcaaaacagcatagctctaaaac"
     gene            complement(866..1528)
                     /gene="csn2"
                     /locus_tag="VCI33_001668"
     CDS             complement(866..1528)
                     /gene="csn2"
                     /locus_tag="VCI33_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEP2832560.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYKYEEDSEL
                     KFFDRKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLLIFVNSGAFLTKDEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLITKN
                     MV"
     gene            complement(1518..1859)
                     /gene="cas2"
                     /locus_tag="VCI33_001669"
     CDS             complement(1518..1859)
                     /gene="cas2"
                     /locus_tag="VCI33_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEP2832561.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
BASE COUNT          648 a          373 c          283 g          634 t
ORIGIN      
        1 attaacaaga ctaatcacac gctctaaggt atcatgagac ggacaacctt cactcaaatc
       61 aacgtaggtc gcaaacaatg gttcattcat ttcaataaaa tcttccatct ccttccaggt
      121 ttcaatgcca gctaactgac aaacgaagac aagaaataga atggttgata aggggtagcc
      181 aattttccaa gattgacgac tatccaattc aactgcacaa tcatcaatag aaataataaa
      241 atcaatcatc gtagtcacct catctatagt gtactacttt gattgatttt gtcattatga
      301 atctagtttt ctgaaaacaa aagtttcttc atgcgtttgg cgtgggtatc cgactgctgg
      361 tattaaccct ctttctcaag ttatcatcgg caatgttgaa tggagaccat tcaaaacagc
      421 atagctctaa aacggaaagc aggagaaagg acgttaatat gtcgttttgg gaccattcaa
      481 gacagcatag ctctaaaacg tcaaactcaa ttttttgtct agctctattg ttttgggacc
      541 attcaaaaca gcatagctct aaaacagaca aaagaaaaag cattgtcaat ttctagtttt
      601 gggaccattc aaaacagcat agctctaaaa caataatcag tttaaaaatc catctggagc
      661 agttttggga ccattcaaaa cagcatagct ctaaaacgag ttaatcgtta accgcagaat
      721 tgacagtgtt ttgggaccat tcaaaacagc atagctctaa aacctcgtag actatttttg
      781 tctaaaaaat ttcgtaatcg cactatttgt ctcagctaga cttcagtctt gaaaagcccc
      841 tgtattactg catttattaa gagtattata ccatattttt agttattaag aaataatctt
      901 catctaaaat atactgcgga aaatcatata gttcacgtgg ttctaaaaag agaactgtta
      961 aatttgtcaa tgatatatac tcttgtaaac tagccacttc atcctttgtt agaaaagctc
     1021 cgctattgac aaaaataagc aactttttct tagtgagata tttgaaaatt tgaagtatct
     1081 ctagacattt ttcaaaaata gtatcacttt gcgtttctac ttttactcct aaggacttaa
     1141 tcaattccag gattgtgatt tcatcatact ctaaatctaa ttcattttct aagcattcaa
     1201 agacaatcag ttctgtaatc gtagcaacca atttgtcaat catcgatttc acttcgggtt
     1261 tctcattaaa ttgagattct aaatctgcat gaatcaattt taaaatggtt gaggagttaa
     1321 catcaaatcc taaaatatct gttacaagca tgatttctga ttctttgatg gttttcatct
     1381 tgcgatcaaa aaatttaagt tcagaatctt cctcatattt gtaacaatat tgcactattt
     1441 ttgaaaatac acagacatct tcgagcacaa gaattgtacc gcctcttaat ggaatcggtt
     1501 catctagtaa ggaaaaatta agattcatca aaagcctccc caagaaatac aagtctttca
     1561 tcggagtttg caatacaatt atttctttca ccatgtaaat aaatcattcg tgcaaactgt
     1621 ttttctgtga ccgttagtaa tgtaatattt cctttattag gattatgctc cctcagccga
     1681 ccaatcatgg cattgttagc tgtattattc aacagcaact tactataaat agaaaattga
     1741 tgcatgataa acccttcact aagtaaaaat ttccgaaatt ttcgataggc ttttcgttcc
     1801 tcagcggtgt ccgtcggcat atcaaacata agtatcattc tcatatatct ataactcata
     1861 tcctaaattc aggaactcct ttcccttcat tattcagagc tttgacaact tttttagtat
     1921 aatcgctaat aatattcg
//
LOCUS       DATUYR010000115         1923 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.115, whole genome shotgun sequence.
ACCESSION   DATUYR010000115 DATUYR010000000
VERSION     DATUYR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.115"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(352..1224)
                     /gene="sdaAA"
                     /locus_tag="VCI33_001670"
     CDS             complement(352..1224)
                     /gene="sdaAA"
                     /locus_tag="VCI33_001670"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEP2832562.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKEQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIDSQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(1236..1907)
                     /gene="sdaAB"
                     /locus_tag="VCI33_001671"
     CDS             complement(1236..1907)
                     /gene="sdaAB"
                     /locus_tag="VCI33_001671"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEP2832563.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGISIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
BASE COUNT          581 a          372 c          339 g          631 t
ORIGIN      
        1 tttaattgtc tttatgattt gtagtgatga attagtctca aaacaaaaca ttgccttatc
       61 agggaatata gctcgtatta tttaggaaaa ctgaccagca cttaagtgcc tctgatattt
      121 aaggcagtta tactgataac aagcaggaaa aaaactctta aatgtttata agatcattag
      181 caattttcag tattttacac tactacactt ttgagtttta acaatttact agaagttgtt
      241 tctaagggga aacaacatag cttgatagct atctattagt cattttagtg aatatatagc
      301 agaaaaataa aacgctttca aatatttcta agaatttttc taactatcta tttattcgcc
      361 aaatatttct acgctgtaac gtcgtccagt tggagtagcg gctaaaccac cttctgctgt
      421 ttcacggaaa gcagttggca tggcagagcc gacttgatac atagcatcaa taacttcgtc
      481 aacaggaatt tgagaatcaa tatcagctaa agccatatca gctgcgacaa gggcgaaact
      541 tgctcctaga gcattgcgtt tgacacaagg cacttctact aggccggcaa caggatcaca
      601 gacaagtcct aataaatttt tgataacaaa cgcaatagct tgactagctt gatgagatgt
      661 tccaccagct gctttaacaa gagcagctgc actcatagca gctgcagaac caacttctgc
      721 ttggcatcca ccttctgcac cagaaataga ggcgttattt ccaataacaa gaccaaaagc
      781 tcctgcagta aataaaaact ctaattgttc tttttctgat aagtcaagtt tttcaatagc
      841 tgttgcgagc actgctggta agcatcctgc acttcctgca gtgggtgttg cacagacaag
      901 tcccatttta gcatttaatt cgtttacagc tatagcattt ctaacagcat taagaatagt
      961 agtatcagaa aggctgttcc cttttttaat gtagttatcc attttgaccg catcgccccc
     1021 tgtcaaacca ctgatagatt tggttgaggt tagtccttct gttacagctg ctttcataac
     1081 ttgtaaattg cgtgacatga ttttgataat atcttctcga tttcttccag acatctccac
     1141 ttccgtggca atcatgagtt cagcaatatt tccattaaat tgttggtcag cttgtttgac
     1201 aagttcttca atagtataaa acatagcttt cctttctagt caaagaagtt gacattataa
     1261 atatgaggaa tttttgcaat ttggttggct gcttcttggc actctctaga atcgacttct
     1321 ataatcatag tagctttttc accagcggat tcacgtgtaa cattcattgt ggcgatatta
     1381 atattattgg aagataggat atcagttacc tttgcgatca ttccgggaat atctttgtgt
     1441 acagtaacta tagtaggggt attcatggat aacgagacag aaaaaccatt tagttctgtt
     1501 acttgaatgt ttcctcctcc aattgaaata ccagtaacac taagtgtttt gtcagctttt
     1561 ttgacactga ttttaacagt attgggatgg ggagcattac tatcttttaa gatatcccaa
     1621 tagattttta tccctttttg atgagcaatt tctagagaat ttttaatatc aggattatca
     1681 gtatccatac ccataattcc tgcaacaaga gccttatcag taccatgtcc tctgtaggtt
     1741 ttagcaaatg aattataaag gtgaaacgtc acttcatcag gaatatctcc aaagatggaa
     1801 tggactacct taccaattct gacggcacca gctgtgtgac tacttgatgg accaatcatt
     1861 actggcccaa tgatgtcaaa aactgattga aatttttgtg tattcatagg caactccgtt
     1921 ttt
//
LOCUS       DATUYR010000116         1875 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.116, whole genome shotgun sequence.
ACCESSION   DATUYR010000116 DATUYR010000000
VERSION     DATUYR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.116"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            8..1303
                     /locus_tag="VCI33_001672"
     CDS             8..1303
                     /locus_tag="VCI33_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2832564.1"
                     /translation="MTKIYIFSFLSDITLPVWKQLTILALALFFNQFPYLSPLLIDPL
                     LFLVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVIPYLFLSSGIYLGHIIFD
                     LLAYLLIFPSFAIINYMIGKDYKMICQSGYSKRSHNFYQTLLIFVLVYYVDIFVILGF
                     TDPFLHFHHSLFVPTPYKLLFLMFILLLVYLLSYFNHSSKEYLKNELRREQQAYMTNL
                     ETYGKHLEKLYRDVRAFQSDYLSRIERLGQAIKSESITQIQDIYAQTVHEANDYWDDK
                     HYNISKLRKINISSIKSLLSAKIISAEKSGIDLNVEVPDNIKETYIPELDLLLLMSIF
                     CDNAIEAALEAQQPHMSIAYFLLDDYQMFVVTNTTKKKVDINKIFEEGYSSKGSERGI
                     GLSNAQRILKKYPYLSLRTKSFDKEFSQTLTMPKEEVDR"
     gene            1300..>1875
                     /locus_tag="VCI33_001673"
     CDS             1300..>1875
                     /locus_tag="VCI33_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEP2832565.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFI"
BASE COUNT          531 a          289 c          327 g          728 t
ORIGIN      
        1 ctattatatg acaaaaattt atattttttc ctttctatct gatatcacat taccagtttg
       61 gaaacaacta acgattttgg ctttagccct atttttcaat cagttcccgt atttgtcgcc
      121 attgctaatt gatcctctat tatttttagt tgttttaagg caagaaacca agcaattatt
      181 ttctttgaaa gctctttttt tggctgttgc acctagtgtt ttggtggatt tgctatctcg
      241 gtttatgggt acaatcgtta tcccctatct ttttttgtcg agtggtattt atctgggtca
      301 tattatcttt gacttgcttg cttatctgct gatttttcca agttttgcta ttattaatta
      361 tatgattgga aaagattaca agatgatatg ccaatccgga tattcgaaac gatcgcataa
      421 tttttatcaa acattgctga tttttgtttt ggtttattat gtggacattt ttgtcatttt
      481 aggatttacg gacccctttt tacattttca tcattctctt tttgttccca caccttataa
      541 gttattgttt ttgatgttta tacttctttt agtttatctt ctatcttatt ttaatcatag
      601 ttctaaagaa taccttaaaa atgagttgag aagagaacaa caagcttata tgactaactt
      661 agagacttat gggaaacatc ttgagaaact ctatcgagat gtaagagctt ttcaaagtga
      721 ttacctgagc cgtatagagc gtttaggtca agccattaag agtgaatcca ttactcagat
      781 tcaagatatt tatgcccaaa cggttcatga agctaacgac tactgggatg ataaacacta
      841 taatatctcg aagcttagga aaattaatat ctcatcgatt aagagcttat tgtcagctaa
      901 aattatcagt gcggaaaaat caggtattga tttgaatgtg gaagtgccag ataatataaa
      961 agagacttac attcctgagt tggacttact attgttgatg tctatttttt gtgataacgc
     1021 cattgaggca gctcttgaag cacagcaacc tcatatgtca attgcttact ttttgttaga
     1081 tgactatcag atgtttgttg tgacaaatac gactaaaaaa aaggtagaca ttaacaagat
     1141 ctttgaagaa ggctattctt caaaaggttc agaacgtggt atcggccttt ctaatgcgca
     1201 gcgtattctc aaaaagtatc cttatttatc cttgcggact aaaagttttg ataaggaatt
     1261 tagccaaacc ttgaccatgc caaaggagga ggtggataga tgatggttat atacagtatg
     1321 gctttattct atgctaccat ctttattaac agctgggtca tttttgcaaa ggtttctgct
     1381 attaaactaa gctggaaacg ggttagcgtt ataggcatta gttttgtaat tgctaatatg
     1441 atatttgaca aagtcatttt gattgaccaa ctgtttttta ttattgttag tttgttaagt
     1501 gctccgaaaa agaaattatt tgagcatatg tttaatggtt tctttactat cttgattgtc
     1561 gaattactgt ttcgcgtcat cggatctttc tttttaccag ctgtcttagg atttagcatt
     1621 ggtcaaatta ataataacct taagctttta gagttatgtt atctttttgt attaccaata
     1681 ttttatctgt ttagctacat tttcagtatt gacttaagct taataaggtt tatcagtgaa
     1741 gataagatga aaaagtgggt attttggatg aatactgcca tgttttctta ctactttttc
     1801 gcccattttt tggtaactgt gcaaagcgga tttcttgcac tatatttcca gtaccggtct
     1861 attttagtat ttatt
//
LOCUS       DATUYR010000117         1864 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.117, whole genome shotgun sequence.
ACCESSION   DATUYR010000117 DATUYR010000000
VERSION     DATUYR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.117"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..504
                     /locus_tag="VCI33_001674"
     CDS             <1..504
                     /locus_tag="VCI33_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832566.1"
                     /translation="LEIDWENVSDDIEPLKIHRNFYESISEKTDSEVIEEIKNLEDIN
                     FTDEKGNDLDDLTIIVPAQDEGFKRVFLGQREWYDIRIGKSKRNRIKYIAGYEVAPES
                     GVKYWAEVDKIVPSDNYVGYWKVIFKGEVKEYSEYKRLGNTWPPQNSRYVSKKALDEA
                     KYLSDVF"
     gene            524..1816
                     /gene="purB"
                     /locus_tag="VCI33_001675"
     CDS             524..1816
                     /gene="purB"
                     /locus_tag="VCI33_001675"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEP2832567.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEELDELFNPIYYTKRVDDIFKRLGI"
BASE COUNT          593 a          324 c          437 g          510 t
ORIGIN      
        1 ttagaaattg attgggaaaa cgtttctgat gacatagagc ccttaaagat acatcgtaac
       61 ttttatgaat caattagtga aaaaacagat tctgaagtta tagaagaaat caaaaattta
      121 gaagatataa atttcactga tgaaaagggt aatgatttgg atgacttaac cattattgtt
      181 cctgcgcaag acgaaggatt taaaagagtc tttctaggtc aacgagaatg gtatgatata
      241 cggataggaa aatcgaaaag gaataggatt aaatatatcg caggatatga agttgcacca
      301 gagagtggtg taaagtactg ggctgaagtg gataaaatag ttccttcaga taactatgtt
      361 ggttactgga aagtaatttt taaaggagag gtgaaggaat attcagaata taaaagatta
      421 ggaaatactt ggccccctca aaacagtaga tacgtatcta aaaaggcttt agatgaagct
      481 aaatatttat cggatgtttt ttaattaaag gagaaaaaac aaaatgatcg aacgttattc
      541 acgccctgag atggcggcaa tttggacaga ggaaaataaa taccgtgctt ggttggaggt
      601 tgagattctg gctgacgagg catgggcaga gttgggtgag attcctaagg aggatgtggc
      661 taagattcgt gagaaggcgg attttgacat tgaccgcatt cttgagattg agcaggagac
      721 gcgtcacgat gtggtggctt tcacgcgtgc ggtttctgaa acgcttggtg aggagcgcaa
      781 gtgggtgcac tacggtttga cctcgactga cgtggtggac actgcctacg gttacctcta
      841 caagcaagct aacgacatta tccgtcgcga tcttgagaat ttcaccaata tcgtggcaga
      901 caaggcgcgt gagcacaaaa tgaccatcat gatgggtcgt acccacggtg ttcacgccga
      961 gccaacgact ttcggtctta agttggcgac ttggtacagc gagatgaaac gtaatattga
     1021 gcgttttgaa catgctgctg caggtgtgga agctggtaag atttcaggtg ccgttggtaa
     1081 ctttgccaac atcccacctt ttgtggaaga atatgtctgt gacaaattag gcattcgtcc
     1141 gcaagaaatt tcaacacaag ttcttccacg tgaccttcac gcagaatact ttgcagtgct
     1201 tgcaagtatt gcaacttcta tcgaacgtat ggcgacagag attcgtggtc tgcaaaaatc
     1261 agaacaacgt gaagttgaag agttctttgc caaaggtcag aaaggtagct ctgctatgcc
     1321 tcacaaacgc aacccaatcg gttcagagaa catgacaggg ctagcgcgcg tgattcgtgg
     1381 tcacatggtg acagcttatg agaacgtgtc actttggcat gagcgtgata tttcacactc
     1441 atcagctgag cgtatcatca cacctgacac aactatcttg attgactaca tgctcaaccg
     1501 ctttggcaat atcgttaaga acttgactgt cttcccggaa aatatgatgc gcaatatgga
     1561 atcaactttt ggtttaattt atagtcaacg tgttatgctc aaattgattg aaaaaggaat
     1621 gacacgagaa gaagcttatg acttagttca acctaagaca gcttattcct gggacaatca
     1681 agtggatttc aaaccacttt tagaagaaga caccaaagtt acctcttgtc ttacacaaga
     1741 agaacttgat gaactattta atccgattta ttacacaaaa cgtgttgatg atatttttaa
     1801 gcgtttaggg atttaatata aaaaaaagaa ggtgattccc ttctttttta tttaaaaaac
     1861 gaat
//
LOCUS       DATUYR010000118         1796 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.118, whole genome shotgun sequence.
ACCESSION   DATUYR010000118 DATUYR010000000
VERSION     DATUYR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.118"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..319)
                     /locus_tag="VCI33_001676"
     CDS             complement(<1..319)
                     /locus_tag="VCI33_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832568.1"
                     /translation="MKKKLVGFIVLALSTIILVACSNDSLEGEYYWINDARNQHMATI
                     KGDKGYVESEGGYSIKIDSELKIIESKFGSEKYSYKDGKLTTNFTGVESDFYKKGSKA
                     CEEA"
     gene            complement(322..660)
                     /locus_tag="VCI33_001677"
     CDS             complement(322..660)
                     /locus_tag="VCI33_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832569.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRLTT
                     KYENKSDNIKSQYLEVIDWAKANLKRRSWIDTIQYYDLEDKGFNIKIIGKLSDRDIER
                     LKDFLRAKEV"
     gene            complement(657..950)
                     /locus_tag="VCI33_001678"
     CDS             complement(657..950)
                     /locus_tag="VCI33_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEP2832570.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNQSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            1007..1156
                     /locus_tag="VCI33_001679"
                     /pseudo
     CDS             1007..1156
                     /locus_tag="VCI33_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1662..>1796)
                     /locus_tag="VCI33_001680"
     CDS             complement(1662..>1796)
                     /locus_tag="VCI33_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP2832571.1"
                     /translation="NQQVKGKGNKLPATGENATPFFNVAALTIISSVGLLSVSKKKED
                     "
BASE COUNT          623 a          339 c          202 g          632 t
ORIGIN      
        1 atgcttcctc acaagctttt gatccctttt tataaaaatc actttctaca ccagtaaaat
       61 ttgttgtcaa ttttccatcc ttataactat atttttcaga accaaattta ctttcaataa
      121 tttttaactc tgaatcaatt tttatggaat aaccgccttc tgattcaaca taacccttat
      181 cgcctttaat tgtagccata tgctgatttc tagcatcatt aatccaataa tattcacctt
      241 caagtgaatc attagaacat gctaccaaaa ttattgtaga taaagctaga acaataaaac
      301 caactaattt ctttttcata actaaacttc ctttgctctg agaaaatctt ttaatctttc
      361 aatatctcta tcacttaact taccaataat tttaatattg aatcccttgt cctctaaatc
      421 atagtactga attgtatcaa tccatgaacg cctttttaaa tttgcttttg cccaatcaat
      481 aacttctaaa tactgacttt ttatattgtc agacttattt tcatatttag tagttaatct
      541 taaagttttt attacttcat cattaaattt gactactaca gcaggacgaa cctttgaacc
      601 tgtaccatca gaatattcaa tacgtgaaac taatacagaa tatacctcaa tatcactcat
      661 ttaaaatatt cctcatattc tgaaaaatct tcatctccaa aaaacgcatt tacactttct
      721 ggatcactag ccttaactcg tattcctttt aattttgttt ctaccaaatt ttgaaattct
      781 tcatcagaaa cagatgattg attttttatt tcaaaaggaa tactttgagt tttaacagac
      841 tgaatcaaaa acatttttac tgctgtactc atatccaacc ctaaggattg aaaaagttct
      901 gtagcatcat cttttaattg atcatcaact cttactgcta ctgttgacat aatatcacct
      961 caaatcattt ttgtataacc attgtatcac taactgcaat acaacgcaaa caaaaaagaa
     1021 aatcgtattt cccagtgaag agaccctaga acactgtcta gtcacactat ttaatgatta
     1081 caacttcaag caagaacaac gagtccacaa aggttttggt cattgtacag acacacttga
     1141 aagcctattt gattaacaat cccagctttg ccagtgtgtt tacacaacat tattgacaca
     1201 ctccttttat ttttataaat tccccttaaa tacatttaat tacttgaaaa gacaaacagt
     1261 cattttagaa aattttgcat ctaaccttac atttgattac acctaaaaat tttaaatcaa
     1321 caaaagagag cctaggatta acatcttagg ctctaatgat acacttcatt tacaaatttc
     1381 aactaaatag gatcatttct tcagttttat agtaagcgtc caataacaag gtatattgga
     1441 tgcttacgga atagggaaga aaatggaaga tacctcgtta ttggacgctt acacaaaatc
     1501 aaaagcctat cgaaattaat aatctcgata ggctttttca agaattaaac tcgaaaaatt
     1561 gaaatacttt aattaagttt gttggagaga gatacataac tttattaatg tgttcacacc
     1621 aataaatggt gaaaagttta gtgacatttt aggtcaaaag attaatcctc ttttttctta
     1681 gaaacagata ataaaccaac tgatgatata attgtcaaag ctgcaacatt aaagaatgga
     1741 gttgcattct cacctgttgc tggtagttta tttcctttac ctttgacttg ctgatt
//
LOCUS       DATUYR010000119         1764 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.119, whole genome shotgun sequence.
ACCESSION   DATUYR010000119 DATUYR010000000
VERSION     DATUYR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.119"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(387..>1764)
                     /locus_tag="VCI33_001681"
     CDS             complement(387..>1764)
                     /locus_tag="VCI33_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEP2832572.1"
                     /translation="QKVSFPKVYLKQGKFDLSVSFEMAGNKFQKRFKDIGGELEDTFF
                     MDFMEKTDDPRFKIYKLAYSAFLARITVNDVVWEKDKGIKLMNGYYWDFINDPHLLVA
                     GGTGGGKTVLLRSILKCLATIGVADICDPKRADFVTMADLPAFRGRIVFEKVDIIAKF
                     ENAITIMYARYDFIRKEMKRLGHKDMGKFYDYGLEPYFFICDEYNALMSSLSYQERDI
                     VENAFTQYILLGRQVGCNAIIAMQKPSADDLPTKIRSNMMHHISVGRLDDGGYMMLFG
                     DENRNKEFRYIKFLSGHRVYGRGYSAVFGEVAREFYAPLLTKKFSFYDEFAKISRHDN
                     PFDPLENTLVSKDIISDESLRKFVEKTSDNELPKGSIGFDFEEEMEEINHMSIGDLAK
                     KTGFSFGKIKNLIDMIEKDGYMTFNREDERVVLTDSDAVILNSLLTQKDKFDGSWKEL
                     LLNYFKGE"
BASE COUNT          583 a          383 c          218 g          580 t
ORIGIN      
        1 acctatacat tttgcaaatt ctttctgctt taagcctgat ttcttcctaa atttctttaa
       61 atacattgga ttcatctatt ttctccctaa atattatcaa tttttagcta ttttttgata
      121 aaatataacc tgtttttgct cgttttataa cctatttttg aaacttgaaa atccttgata
      181 catatggggt ttcgggggtt ttacttcgta gatttttcaa atcttacccc cctattagaa
      241 atcgggggtt ttaaaattga taaatccctc tgtaaagccc tagtgtgtgc gctgtgtccg
      301 gcgtgtccgt ttccgctacg ctacactacg cctacagcta cactagggct ttacagaagg
      361 atatttaaaa aaataccctt catctactat tcacccttaa aataattcaa caacaactct
      421 ttccaactgc catcaaactt atctttttga gtgagtaaac tattcaatat aactgcatca
      481 gaatcagtca atactactct ttcatcttca cgattaaaag tcatgtaacc atctttttca
      541 atcatatcaa ttaaattttt tattttacca aatgaaaaac ctgttttctt agctaaatca
      601 ccaatactca tatgattaat ttcctccatt tcctcttcaa aatcaaagcc aatacttcct
      661 ttaggtaact cattgtcaga cgttttttct acaaattttc tcaatgattc atctgaaata
      721 atatccttgg aaaccaaagt attttcaagc ggatcaaatg gattatcatg acgtgagatt
      781 ttcgcaaatt catcataaaa tgaaaatttc ttagttagca aaggagcata aaactctctc
      841 gctacctcgc caaaaacagc agaatagcca cgaccataca cacggtgacc tgatagaaat
      901 ttaatatacc tgaactcttt attccgattt tcatccccaa aaagcatcat ataaccacca
      961 tcatcaaggc gaccaacaga aatatggtgc atcatatttg acctgatttt tgttggtaaa
     1021 tcatctgcgg atggcttttg catggcaata atcgcattac agcctacttg acggccaagt
     1081 agaatatact gtgtaaaggc attctcaaca atgtctcttt cctgatacga taacgaagac
     1141 atcaaggcat tatattcatc acaaataaaa aaatatggtt ctaatccgta atcataaaat
     1201 ttccccatat ctttatgccc taaacgtttc atttccttac gaataaagtc atagcgtgca
     1261 tacatgattg taattgcatt ctcaaattta gcaataatat ctactttttc aaaaacaatt
     1321 cttcctcgaa aagcaggtaa atcagccatt gtcacaaaat cagctcgctt aggatcacat
     1381 atatccgcaa cgccaatagt tgcaagacat tttaaaattg atctaagcaa aacagtctta
     1441 ccgccaccag tcccacccgc aacaagcaaa tgaggatcat tgataaaatc ccaataataa
     1501 ccattcatca gcttaatgcc tttatccttt tcccatacaa catcattaac cgttatacga
     1561 gccagaaagg cggaataagc taatttataa atcttaaacc taggatcatc agttttttcc
     1621 ataaaatcca taaaaaaagt atcttcaagt tcacctccaa tatctttgaa acgcttttga
     1681 aatttgtttc ctgccatttc aaaggagaca cttaaatcaa acttaccttg ttttaaataa
     1741 acttttggaa aactaacttt ctgc
//
LOCUS       DATUYR010000120         1749 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.120, whole genome shotgun sequence.
ACCESSION   DATUYR010000120 DATUYR010000000
VERSION     DATUYR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.120"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            8..1726
                     /locus_tag="VCI33_001682"
     CDS             8..1726
                     /locus_tag="VCI33_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP2832573.1"
                     /translation="MTVFLVIFLIYFNNFFVKKRSQELGVLAILGFSKKELTQLLTLE
                     NLVILVLSYLVSLLLGPTLYFLAVLVITHLLELTLEVQWFITANEIIESLGILVVVFL
                     INVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAIIAITALLLSYILCLATVFSST
                     RNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFIVSLLKGNKKRLYRPLSNIIYPKF
                     NYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEYNVKSENGQV
                     NVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFDIINYSDYKELMKAQGRIN
                     SIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTHNVFSFSTSVTTLVVSDK
                     LYAKLSSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDVISSYSKEYTVKTANIATY
                     IFITFLSILFIICTGSILYFTSLIEIMENKEEYGYLSKLGYSKKMIHRIPRYETGILF
                     LIPVFIGIVNGGMLLIYYKYLFMDTLVAGNIIMLSLLLCLLFFLIIYGTFYVLTLRSV
                     TSIIKN"
BASE COUNT          573 a          239 c          297 g          640 t
ORIGIN      
        1 agtttttttg actgtatttt tggtgatatt tttaatttat ttcaataatt tctttgtaaa
       61 aaaacgtagt caagagcttg gagtcttagc aatactaggg ttttcaaaaa aagaattaac
      121 acaattactg actttagaaa atcttgttat tctagtttta agttacttgg taagtttact
      181 attgggaccg accttatatt ttttagcggt actggtaatt actcatctat tggaattaac
      241 attggaagtt cagtggttta ttacagctaa tgagattata gagtctttag gaatattagt
      301 cgtagttttt ctgattaatg tcatcacaaa tggacttatc attagtaaac agtctttgat
      361 tgaatttgtt aatttctcaa gaaaggctga gaaaaaaatt aagataagaa aagtcagagc
      421 tattattgct atcactgcat tgctattgtc atatatttta tgtttggcga cagtgttttc
      481 atccacacga aatatgctat taagcatagg gatggtaccg gtttctctat tgataattgt
      541 cttagttgtt ttaggaacag tgttcaccat cagatatgga ttggctttta tagtttcgtt
      601 attaaaagga aataaaaaaa ggttataccg tcctctgtct aatatcatct atcccaaatt
      661 taactatcgt attgcaacaa aaaataaatt attaacagtc ttgggaggtc ttttaacagt
      721 aaccgtttca gttgctggaa tgatggtaat gctctatgct tattctctta atgggataga
      781 gaggttgact ccatctgcca tagaatataa tgttaaatca gaaaacggtc aagtcaatgt
      841 tacaactatt ttagagaacg accaagtgag cttggttgat gttgacctat tgagattgaa
      901 cactaaccca gaagtaacta tcacagaatc tgggcaaaca attccttatt ttgatataat
      961 taattacagt gattataaag agttaatgaa agctcaaggg agaataaatt ctattgaagg
     1021 tagtaagtca ctcccattgt taataaatta ttatccaaca gaaattagcc ttggaaaaac
     1081 ctttaactta ggaaatgcat atgatgttac tgtaaaacaa gtatcaacgc ataatgtttt
     1141 tagtttttct acaagtgtca cgaccttggt tgtttctgat aaattatatg ctaaacttag
     1201 ttcttgtttt ccagagaaag aaatgacaat taggactttt aatggaaatt cgattaggtc
     1261 aagtgaagca ttttacaatc agtttagtat ggttcctgat gttatcagta gttatagtaa
     1321 ggaatacaca gtaaagactg ctaatattgc gacttatatc tttataactt tcctatccat
     1381 actctttatt atttgtacag gtagtattct gtactttaca agcctcatcg aaatcatgga
     1441 aaataaagaa gaatatggct atctaagtaa gctaggttat agtaaaaaaa tgattcatcg
     1501 gattcctcga tatgaaacag gtatactttt ccttattcct gtattcattg ggattgtaaa
     1561 tggtggtatg ttgcttattt actataaata tttattcatg gatacattgg tagcaggcaa
     1621 tatcataatg ttatctttat tgctttgtct gcttttcttc ttgataatat atggcacatt
     1681 ttatgtattg acattgcggt cagtgacatc cataatcaaa aattaatttt aaaaatcttt
     1741 taactttaa
//
LOCUS       DATUYR010000121         1727 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.121, whole genome shotgun sequence.
ACCESSION   DATUYR010000121 DATUYR010000000
VERSION     DATUYR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.121"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(54..1331)
                     /locus_tag="VCI33_001683"
     CDS             complement(54..1331)
                     /locus_tag="VCI33_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEP2832574.1"
                     /translation="MSEKIVVGIDVSKAFSDICILSPNNDIIKRLKISNDINGMKSLI
                     YVLEKVEDEYKDRAVIIMEATAHYHQILANFFRKHNYEVIVINPIQSGALKNINIRKI
                     KSDKTDAYNIALLYRIKNYNETITHSDTVNSIKKLCRQHKELTDEIVEHINRLIAFLD
                     ISFPDFKKVFADLQGKTPLSLLEKYPTIQEVLSPENKQDIIQLIKENSHKSSSYAEVK
                     YEKLLKAAEKSIEVCIVSLSSAVLIQTTVRVIFSLQEALKAIDDEIKRLSLLDEKFHK
                     EIVLLQSIPGVGEYTACVVLSELGDISNFSKPKELVAFFGLDPGVTQSGTYNRKNNKI
                     SKRGSPHVRSILHMLAKSNVYPNRNREYLNPIMRAYFEKKIAEKPYKVVMCAIMRKMV
                     QIIFAVLRNQKSFELRTPEEHQKLIREKSKLVA"
BASE COUNT          547 a          298 c          264 g          618 t
ORIGIN      
        1 atagcaaata agcctcatat actttcattt tttgaggcaa cacttttaat agtttatgcg
       61 actaacttac ttttttctcg aattagtttt tgatgttctt cgggtgttct taattcaaat
      121 gatttttggt ttctgagaac agcaaaaata atttgaacca tctttcgcat aattgcacac
      181 attacaactt tgtatggttt ttcagctatt ttcttttcaa aataagctcg cataatagga
      241 tttaaatact ctctgttgcg atttggatac acattagatt tagccagcat atggagaatt
      301 gagcgaacat gaggtgatcc tcttttagag attttattgt ttttcctatt gtatgttcca
      361 ctttgggtta ctcctggatc caatccaaag aaggcaacta attctttcgg tttcgagaaa
      421 ttggatatat ctccaagctc tgataaaacg acacatgctg tatactctcc aacgcctgga
      481 atactttgta aaagtactat ttctttatga aacttttcat ctaaaagaga tagtcttttg
      541 atttcatcat cgattgcttt caaagcttcc tgtaaactga aaataaccct cacagttgtt
      601 tgaataagta ctgctgaact aaggcttact atacaaactt caatagattt ttcagcagct
      661 ttcaatagtt tttcatattt tacttcggca taggagctgc ttttatgtga attttctttt
      721 atcagttgaa taatatcttg cttattttca ggagataata cttcctgtat agtaggatat
      781 ttttcaagca aagacaaagg tgttttccct tgcaaatccg caaatacttt cttgaaatct
      841 ggaaaggaaa tatctaaaaa agcgattaat cggttgatgt gttccacaat ttcatccgtc
      901 aattctttat gctgtctgca aagctttttg atactgttaa cggtatctga gtgcgtaatt
      961 gtttcgttgt aattcttaat acgatatagc aacgctatat tatatgcatc tgttttatct
     1021 gatttaattt ttcggatatt gatatttttc aatgctccac tttgaatcgg attaattaca
     1081 atgacttcgt aattatgctt acggaaaaaa ttagctaaaa tttgatgata gtgtgcagta
     1141 gcttccatga taattactgc tctgtctttg tactcatctt ctaccttttc aagtacatag
     1201 atgagcgact tcataccatt aatatcatta gaaattttaa gtcttttgat gatgtcatta
     1261 ttaggtgata gaatacagat atcactgaaa gctttactca catcaatacc tacaacaatt
     1321 ttttctgaca taatatacct cctataattt gggatgccct gttaatccaa aaccacaaca
     1381 ggctcacgag ttatacaggt aatcaataaa tgaacctaac caaccgaaaa gatgaaaggc
     1441 aatggaatga gggtggagtc taccaaatga gtaattccta agttggaaac ccagcaagca
     1501 cataaaacaa tcccatttca tccctaatat ctataatttt atcatagatg attaggttca
     1561 tttttaagaa ctatgcttaa agctgttctt atactattag tatatgagca agcacagtaa
     1621 gtgagtaccc acttacgaaa aaatcaaaaa atcgatagct tttaaggcta tgtaattttt
     1681 aaatttttac cttgttaggg agaaccctaa gaccccaaaa aataaaa
//
LOCUS       DATUYR010000122         1714 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.122, whole genome shotgun sequence.
ACCESSION   DATUYR010000122 DATUYR010000000
VERSION     DATUYR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.122"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            134..775
                     /locus_tag="VCI33_001684"
     CDS             134..775
                     /locus_tag="VCI33_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEP2832575.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWKSIQPNEEVDKVFIVPLRQLLFTDPVYYRLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPSDIENSKGR"
     gene            complement(822..1664)
                     /locus_tag="VCI33_001685"
     CDS             complement(822..1664)
                     /locus_tag="VCI33_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEP2832576.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVSVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKADWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAFVLFALGIWLGLWMSQLE
                     EKYK"
BASE COUNT          618 a          300 c          332 g          464 t
ORIGIN      
        1 tatgtcaata ttttatatat aaaaaaataa catatatatc aaaaatcact aactaggagt
       61 aataaattga ccaaaaagct aggattggtt atgatgaaga taagattaac ggaaagagaa
      121 attagggaag gctatgaagg acttattaaa acagtatcag gcaaaaccac taggagagga
      181 gaagcggtac gccgtatttt tacctcttat tcttgtcaat gatgactggc atgtcttata
      241 tgaagtcaga agtcagcata tttcacaacc gggggaagtt tctttcccag ggggacaagt
      301 agaaaatcag gagacacttc aagaagctgc tatccgagaa acagttgagg aattgactgt
      361 tgatgcgtcc cagatccagc tatggggtga aattgattat ttagtacagt cttctagaac
      421 tattcattgt ttcgttggtc aattagtcgt tgatgactgg aaaagcatac agcctaacga
      481 agaagttgac aaggtcttta tagtaccttt acgtcagcta cttttcactg atcctgtcta
      541 ttatcgttta gaagccactc ctattgagac aacggatttt ccttttgacc gtattcgtaa
      601 tgggaaatac taccaattta gtcaagagta ccgtagtatt ccgttttatg aaaatctaga
      661 agaaaccata tggggaatga ctgcccagtt tactaagtgt ttaaccgata ttctaaatga
      721 tgaatcaatt gcaaagaaaa acccctcaga tattgaaaat tctaagggga gatagagcac
      781 tagtttgttg gcgtcaactt tttgtcaaca aactgtaaag gttatttgta tttttcttct
      841 aattgactca tccaaagtcc gagccaaata ccaagagcaa ataacacaaa ggccataagc
      901 caggttaaga tacctgcatg actgtaggaa atggattcgg gactactact atttggaata
      961 agaattaata gcgtgctgga taatacaata ccaatgataa agtggtaaac ttttgaatga
     1021 tggtgctgta aagcataatc catactttta gaaaaagcca agatggctag gactcctccg
     1081 atagcaatag gtaagaaagt ccctaataaa tccaaagatt tgaaccctat taacatagga
     1141 ccatagagtc ctaagataag taagagattt gacgggctta gtccagggac cagtactcct
     1201 aaagcaatta aggcacctgc taaaataaag gttaaaaagt tagctggtaa ggtaccgatt
     1261 aagtcattta agaagtaaag acctagtcct gaaataacaa aggtaaggac tagccatagc
     1321 cagtcagctt tgtctctttg agactgcttg gttgactcct taatcagact aggaatagtg
     1381 ccgacaatag cgccagcaaa tccccataag acgctaactt gatagtgctt aagcaaaaac
     1441 tcaacgggga aagaaaagag agcaatacct aaaataccac caattcctac tggtaagaaa
     1501 aataagacat tttcaataaa gttatccctc atatgagcta aaaaggaaat cattcgctca
     1561 tagattccta aaatggctgc gagaacacca ccggatacac caggtaaaat aaaacctagt
     1621 gcaataatca tgcctttaaa aactcttgaa ataaatgaaa ccattgttgc ctcctgaaaa
     1681 gaatgatttt attgtatcat aaatttaaag aatt
//
LOCUS       DATUYR010000123         1681 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.123, whole genome shotgun sequence.
ACCESSION   DATUYR010000123 DATUYR010000000
VERSION     DATUYR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.123"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            182..>1681
                     /locus_tag="VCI33_001686"
     CDS             182..>1681
                     /locus_tag="VCI33_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEP2832577.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTCFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMGNVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKASHRKILEDELLENKAAYIVVNRYKNLPKIIQKVLST"
BASE COUNT          483 a          266 c          292 g          640 t
ORIGIN      
        1 ataatatcat ttttcaactt actttgttta tgcactgtca ctttgctatg tgcaatacaa
       61 agtcatgtta tttatctcat actaaaaaaa taatggataa tagctatgtt agactagtga
      121 tatgaagaaa tattgctttt attgaatcgt tagagaatat gatatttcaa ggagaaaaat
      181 agtgatcgag ttaaacgatg aattcatccg aaaagagacg atagaactgg ccaacgatgg
      241 cccacgagta cacaccactc aatatgaaac taaagtacct cggcttcata aatgctatct
      301 gcttttcttt agcattatta tcagttcttt aacaattgca gtaccttttt taacagatgc
      361 agccaatggt cttcagtccc aaaacttata tataggaatg atgttaacga aggggcaact
      421 tccctatagt gatgctttta caacaggagg gctcttttac tttgttatca ttgcattaag
      481 ttattatttg ggatcgacac tttggctagt ctttgttcag gtgttttgtt tttacttatc
      541 tggtttatat ctttataaac tcattaatta tatgacatgc tttcaaaaag tggctttaac
      601 tttttcaatt agctactatt tgttgtctgt tagtcttggt tttggaggat tgtatcctac
      661 tcaactggct atgccattta tattaatatc ggcttggttt ttaactaagt attttgcctg
      721 tttagtgaaa gatgaggcat ttattctttt tggctttgta ggtgctcttg caatgctaat
      781 tgacccgagt acccttatct tttggtcttt tgcttgtgtg acagtttttt cttataatat
      841 aagccaaaag catcttgcaa gaggttttta tcaactgcta gcttcgattt ttgggatgat
      901 tttagttttt tacacagcag gatatttcat tttgaactta caagtgctaa atccttattt
      961 atcacaaacg atgatttatc cttttacttt ttttaaatca ggaaacttat cgttgctttt
     1021 tggactggct attcagttgt tcttcgcttt ggggcttggt cttttgacag gaatggggaa
     1081 tgtcattagg cgatttaaaa acaattctga tagggtcgtc aagtggctat ttgtcatggt
     1141 cattctagaa tctatacttg tggctatatt ttcacaagac tatcgcccct atcatctttt
     1201 acctctttta ccttttggat taattttgac tgctattcct gttggctatc agtatggtat
     1261 aggattaggt cagagtagtc atcgcagacg tcatggtaaa aatggtgttg gtcgagtaat
     1321 gatgatttat cttaagagac acttttattt gccaatttta attgtaggga caatactaat
     1381 ctgttctact tattgtttca ttagtagtat tcctcttaat caggagcgtg atcatattgc
     1441 tagttattta gaacagaaac taaataaaac tcaatctatt tatgtttggg atgatacttc
     1501 taaaatttat ttggacagta aagctaaatc tgtttctcaa tttagttctc ctgacatcaa
     1561 tacgcaaaaa gcgagtcatc gaaaaatatt agaagatgaa ctattagaaa ataaggctgc
     1621 ttatatcgtt gttaatcgct ataaaaacct gcctaaaatc attcaaaaag tattatctac
     1681 t
//
LOCUS       DATUYR010000124         1601 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.124, whole genome shotgun sequence.
ACCESSION   DATUYR010000124 DATUYR010000000
VERSION     DATUYR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.124"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            143..685
                     /locus_tag="VCI33_001687"
     CDS             143..685
                     /locus_tag="VCI33_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832578.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            809..1588
                     /locus_tag="VCI33_001688"
     CDS             809..1588
                     /locus_tag="VCI33_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEP2832579.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFATNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
BASE COUNT          422 a          243 c          327 g          609 t
ORIGIN      
        1 ttgatggttg ttatgaatac catgctaaaa tattctattg attatttatc agcaacaagg
       61 tatcgtttaa aaataccaaa agatacaggt gtcttgatag ttattggttt ttaaggtata
      121 ctatttttga aaaggagata aaatgactaa aaaaccaaca caactaatag cttatacctc
      181 tattttggtc gcatttgcga ttttgattcc gataatcatg cctcttaagc ttattattgg
      241 accagcttcc tttactctgg cgagccatgt tcctttgttt ttggctattt ttatgtcgat
      301 tccagtagca attttggtcg ctcttggtac cacattaggt tttttattag ctggacttcc
      361 tttaattatt gttttaagag cactctctca tttattgttt gctattttgg cagcgtggtg
      421 gttaagtaga aaaccacaac tgatgacatc ggcagtaaaa tgttttagtt ttgcgttttt
      481 cattaatgtc attcatggct tggctgaatt tttagttgtc tatattttaa cagcaaccac
      541 agcaacttct atgtcttatt tttggtctat gctaggcttg attggcttag gttcgcttat
      601 tcatggcata ttggatttct atcttgcttt agttctatgg cgttttttag caaaaaactt
      661 aaaacttccc accataaatg cctaagacta aaatagagat agttatatga taatgacgag
      721 ccctttcttg ttgaaagggc ttgcattttt taggaaaatt gatatactaa agtgtgtgtt
      781 tgtgaataca gtaacaaaag gagatatcat gaaaacacca gaacagattt tagaagcaac
      841 tattcatatt ggggaacata aggttaccaa aacttttttg gctaaatcta tcttaggctt
      901 tattggtgga gcgatgatta gccttggtta tttactctac gtgagaattg ccgccagtgg
      961 tttagaaact tttggtgctt tttccagtat cgtaggagct tgtgcttttc cgattggtct
     1021 aattattatt ttaatggcgg gtggagaatt aattacaggg aacatgatgg ctgtttctgc
     1081 agctttgttg gccaaaaaaa ttaagtttag cgagcttgcc aagaattggc ttatcattac
     1141 cttgtttaat gtgattggag ctgtttttgt agcttttgtg tttggtcatt ttctaggctt
     1201 aacatcagct ggtattttta aagaagaagt gattgaggtg gcccatgcta aaatagcagc
     1261 gagccctttg caggcactag tatcaggaat tggttgtaac tggtttgttg gtttggcctt
     1321 gtggctatgc tatggggcaa atgatgctgc tggtaaattc cttggtactt ggtttcctgt
     1381 catgaccttt gtagcccttg gttttcagca tagtgtagca aatgcttttg tcattcctgc
     1441 tgctattttt gaaggaggag caacatggtt ggattttgcc actaatttta tttttgtcta
     1501 tagtggtaat attatcggtg gagccatttt tgttagtttt ttatatttta aagtttacta
     1561 tcatccgcaa aagagtaaaa ctcaatgaga aaatattttt t
//
LOCUS       DATUYR010000125         1596 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.125, whole genome shotgun sequence.
ACCESSION   DATUYR010000125 DATUYR010000000
VERSION     DATUYR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.125"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            20..940
                     /locus_tag="VCI33_001689"
     CDS             20..940
                     /locus_tag="VCI33_001689"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEP2832580.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            994..1242
                     /locus_tag="VCI33_001690"
     CDS             994..1242
                     /locus_tag="VCI33_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEP2832581.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1367..>1596)
                     /locus_tag="VCI33_001691"
     CDS             complement(1367..>1596)
                     /locus_tag="VCI33_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP2832582.1"
                     /translation="DKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTGGFGLG
                     LALAQQIVMSLKGNITVKDNDPKGSIFEVKL"
BASE COUNT          561 a          301 c          228 g          506 t
ORIGIN      
        1 caaatgatta ggagctacca tgtcaaatga atttatcaac tttgaaaaaa tttctagaga
       61 gtcttggaaa acattacatc aaaaagcaaa agctcttctc acacaagagg aacttaaaag
      121 tattactagc ttaaatgata acatcagtat taacgatgtc attgatatct accttccatt
      181 aattaatctg atccaggttt acaaaattgc gcaagaaaat ctttcttttt ctaaaagcct
      241 ttttctaaaa aaagacattc aattaaggcc ttttattatt ggaatttctg gttcagtagc
      301 tgttggtaaa tcaacaacta gtcgtctttt acaactttta ttatcacgaa cgcaccccaa
      361 tagtcaagtc gaactggtaa cgactgatgg ttttttatat cctaatcagt ttcttattga
      421 acaaggactg ttaaatcgta aaggatttcc agaatcttat aacatggaac ttctcttaga
      481 ttttctagat tccatcaaaa atggtcaaac ggcttttgca cccgtctatt ctcatgatat
      541 ttacgatatt atcccaaatc aaaaacaatc atttaataac cctgacttcc ttatcgtcga
      601 aggtattaat gtttttcaaa atcaacaaaa caatcgttta tatatgagtg attactttga
      661 tttttctatt tatattgatg cagacagcag tcacatcgaa acctggtaca ttgaacggtt
      721 tctaagcatt ttaaaattag caaaacgtga ccctcataat tattatgccc aatatgcaca
      781 attacctcga tctgaggcta tagcatttgc gcgcaacgtt tggaaaaccg ttaatttaga
      841 aaatcttgaa aaattcattg aacctacacg taaccgtgct gaattaattt tacataaaag
      901 tgctgatcat aaaattgatg aaatttacct caaaaagtga taaagggttg ccaaacgcat
      961 atattttcta tataataatg tagttagatt aatttggagg tgaaaacatt ggcaaatatt
     1021 aaatcagcta ttaaacgcgc tgaacttaac gttaaagcaa acgaaaaaaa ctcagcacaa
     1081 aaatcagcta tgcgtactgc tatcaaagca tttgaagcta acccatctga agagcttttc
     1141 cgcgctgctt cttcaagcat cgataaagct gaatcaaaag gtttgattca caaaaacaaa
     1201 gcaagccgcg ataaagcacg ccttgctgca aaacttggct aataaatatc caatgaaaac
     1261 tccagctgga gtttttttat acggtaaaaa ctcaaaaaat acaataaagc gttgacagtt
     1321 aaaaataact ttaaaagggc tgaccttatt ggtcgccctt tttagcttat aatttgactt
     1381 caaaaatact acctttagga tcattatcct ttactgtaat atttcctttt aaagacatca
     1441 cgatttgctg agctaaagcc aaccccaagc caaatccacc tgtttgccgt gttctagctt
     1501 tgtcaactcg ataaaaacga tcaaaaatct ttttcttttc ttcatctgtt atccctggac
     1561 cattatcaat aacagaaatt aataaatttt tgtccg
//
LOCUS       DATUYR010000126         1578 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.126, whole genome shotgun sequence.
ACCESSION   DATUYR010000126 DATUYR010000000
VERSION     DATUYR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.126"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            134..1330
                     /locus_tag="VCI33_001692"
     CDS             134..1330
                     /locus_tag="VCI33_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEP2832583.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
BASE COUNT          490 a          265 c          337 g          486 t
ORIGIN      
        1 tgaaaatagg gttaatgact gaaaaaaatg ttgattttgt gaaatgatta ttttattatt
       61 ttcatatttt atctttatgc taggaattgt ggtataatgg aggagtataa aaaatattaa
      121 ggagtctatt cacatggcta aattgactgt taaagacgtt gatttgaaag gtaaaaaagt
      181 cctcgttcgt gttgacttta atgtgccttt gaaagatggc gttatcacta acgacaaccg
      241 tatcactgcg gctcttccaa caatcaagta tatcatcgaa caaggtggtc gtgctatcct
      301 cttctctcac cttggacgtg ttaaagaaga agctgacaaa gaaggaaaat cacttgcacc
      361 ggtagctgct gatttagctg ctaaacttgg tcaagatgtt gtattcccag gtgttactcg
      421 tggttcaaaa ttagaagaag caatcaatgc tttggaagat ggacaagttc ttttggttga
      481 aaacactcgt tttgaagatg ttgacggtaa gaaagaatct aagaatgacg aagaacttgg
      541 taaatactgg gcttcacttg gagatggaat cttcgttaac gatgcatttg gtacagcaca
      601 ccgtgctcac gcatcaaacg taggtatttc agcaaacgtt gaaaaagctg tagctggttt
      661 ccttcttgaa aacgaaattg cttacatcca agaagcagtt gaaactccag aacgtccatt
      721 cgtagctatt cttggtggct caaaagtttc tgataagatt ggtgttatcg aaaatcttct
      781 tgaaaaagct gataaagttc ttatcggtgg tggtatgact tacacattct acaaagctca
      841 aggtatcgaa atcggtaact cacttgtaga agaagacaaa ttggatgttg ctaaagacct
      901 ccttgaaaaa tcaaatggta aattgatctt gccagttgac tcgaaagaag caaacgcatt
      961 tgctggttac actgaagttc gcgacactga aggtgaagca gtttcagaag gcttccttgg
     1021 tcttgacatc ggtcctaaat caatcgctaa atttgatgaa gcacttactg gtgctaaaac
     1081 agttgtttgg aacggaccta tgggtgtctt tgaaaaccct gacttccaag ctggtacaat
     1141 cggtgtcatg gacgctatcg ttaaacaacc aggcgttaaa tccatcatcg gtggtggtga
     1201 ttcagcagca gctgctatca accttggccg tgctgacaaa ttctcatgga tcagtaccgg
     1261 cggaggcgct agcatggaac ttctcgaggg taaagtatta ccaggtttgg cagcattgac
     1321 tgaaaaataa tagtcattat aatatatagt aaagggcatg ctgatgcatg tcttttttta
     1381 gttgatcatg tacagttatt gaactttccc taacttcact tatcatttac tagtttttag
     1441 atatagtggt atttggctga cactgcaatt ttcaaaagtt tatgacgatt tcatatcaat
     1501 tggtttataa aagatatcct aatcataaat aaaacgttta ccaaaagggt tttatggatt
     1561 gatatgttaa aatagata
//
LOCUS       DATUYR010000127         1555 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.127, whole genome shotgun sequence.
ACCESSION   DATUYR010000127 DATUYR010000000
VERSION     DATUYR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.127"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>1555)
                     /locus_tag="VCI33_001693"
     CDS             complement(<1..>1555)
                     /locus_tag="VCI33_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEP2832584.1"
                     /translation="LDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQ
                     EISFYLFRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEF
                     IVGELSKITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKH
                     VLLWMKLFAINGLTLVVVSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTI
                     FFKLCSAMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSK
                     TVIAHRGLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVN
                     KTIRNLTLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVD
                     LFLETYHRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVI
                     EDFSYRSYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLK
                     QDNSYFSRLV"
BASE COUNT          571 a          269 c          237 g          478 t
ORIGIN      
        1 gactaatcga ctaaaataag aattatcttg ttttaagtct ttaatcaatt gattagttaa
       61 agctggttga tctgtaataa ttccctgaat aggctttagg agataatgct ctatgcgctt
      121 gggatcatta atagtccaaa cgtaaatttc tttattattc caaaaagctt gggacgacaa
      181 ataagaccga taagaaaagt cttcaatgac atagaaatca acaaattcat ctccaaaaaa
      241 gccaaattga attggtatga tataaccagt cgtaattgat ggattttttt tcttgatagc
      301 ttctattact tctaaatcta aagacatgac tttattatct ttgcttatac caagtcgatg
      361 ataagtttct aaaaacaaat cgacataatt tccaggttct gtaccaattg gcttgagttc
      421 aataagtaat ggcatattca acttcttagc cttttgataa aaagtgtcaa aagaaacaaa
      481 acgccctgaa aaatgtcctt gatgactcgt tagatgttca acttctttta aggttaagtt
      541 gcgaatcgtc ttatttactc cagctaaacg cttcaatcga ttatcatgag acaccacaaa
      601 gtgattatcc ttagtcaaga ttagatccag ttctacataa tcacttcctg ctttcttagc
      661 accttcaagg gcttccagag aattttctac acctgctgat acaagtcctc tatgcgcgat
      721 aactgtctta gagtgagatg tgtcaaaaaa agttaaacgt tcaagagatt gataagcaaa
      781 acctactgtc accacgataa agattacaac atatgcctta ttacttcttc ttggctcatc
      841 atattgcttt tgtggttcaa ttgcctcttt taaaatcatt gctgaacaga gtttaaaaaa
      901 aatagtagta aaaaaaatga gctcccatgt aaaggtcaaa gctcccagct gaacaataat
      961 attattcccc ttaggattaa acatatcaac aaaaataaga atcatggaaa tagctagcga
     1021 tacgactaca agcgtaagac cattgattgc aaaaagtttc atccataaca atacatgttt
     1081 ctttttagtc ttttgccaac tctctctcca tgcttgagca accgtacgat ggttgattgc
     1141 tatcaatggt aaaaaatata ctaatcttag gttaaggtaa aatacaagaa taagactgcc
     1201 ataaagcaag tactttgtgc tagttatctt tgataattcc ccaacaataa attctggaag
     1261 ataaagcttt tgagtaatca cagaagataa accaatatgt aagactggaa tagtcaatag
     1321 gatataaagc aaaaaaagta ataattggta accagaaaat gttttccaaa gcccccttag
     1381 gtaataagaa aactgttttc taaaaagata gaaactaatt tcttgatccg caataattcg
     1441 atatagagca aaaaactcaa ggtgaataaa agcagctaat aaaaataaat aggcacctaa
     1501 taaagcaagt gctataggat tttttaaaaa agtcacaaca ttgtctttat ctaaa
//
LOCUS       DATUYR010000128         1518 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.128, whole genome shotgun sequence.
ACCESSION   DATUYR010000128 DATUYR010000000
VERSION     DATUYR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.128"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..573
                     /locus_tag="VCI33_001694"
     CDS             <1..573
                     /locus_tag="VCI33_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEP2832585.1"
                     /translation="NPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTS
                     YARLLSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFI
                     WSNGTNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKA
                     IQNDLKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            752..1447
                     /locus_tag="VCI33_001695"
     CDS             752..1447
                     /locus_tag="VCI33_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP2832586.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            1449..>1518
                     /locus_tag="VCI33_001696"
     CDS             1449..>1518
                     /locus_tag="VCI33_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEP2832587.1"
                     /translation="MTVTASLFISFIVLTFVFFLINL"
BASE COUNT          513 a          224 c          280 g          501 t
ORIGIN      
        1 aatcctagtg aaagccaatt tttctcagtt attacaatgc aaaatcatat tccttggtca
       61 tccgattatc ctgaagaaat tgttgctgaa ggaaaaaatt tcacggaaga agaaaatcac
      121 aacctaacaa gttatgctcg gttattatcg tttactgata aggaaacaag agcattttta
      181 gaaaaattaa cacaaattaa caagcctatc acagtggtgt tttacggaga tcatttaccc
      241 ggtttatatc ctgatagtgc ttttaacaag catattgaaa ataaatacct tactgattat
      301 tttatttgga gtaatggtac taacgagaaa aaaaatcatc cgcttatcaa ctcaagtgat
      361 tttactgcag ctttatttga gcatactgat tcaaaagtat caccttacta tgctttgtta
      421 acagaggtac tgaataaagc tagtgtcgat aaatcaccag atagtcctga agttaaagct
      481 attcagaatg atttaaaaaa tatccaatac gatgtgacta taggaaaagg ttaccttttg
      541 aaacacaaaa ctttttttaa gatatcacgt taagaaaaat gctaggctgt ccgagtatag
      601 acgagcatca aggtttggta atttgctcaa atgattgtca acagagtgtc taggaccagg
      661 tttaaaagat tataattctt aaggtcttat tcgtttataa aaaatgaata ctcttttaaa
      721 atcttaattg aaatgaatag gagaagtttt cgtgaaaaag ttaatcatta ttcctgctta
      781 caatgaaagc agtaatattg tcaatactat acgtactatt gaatcagatg ccccggattt
      841 tgactatatc attattgatg attgctcaac ggataatacg ttagcaatat gtcaaaaaca
      901 ggggttcaat gttatttctt tgcccattaa cctgggaatt ggcggtgcgg tgcaaactgg
      961 ctatcgttat gcacaaagat gtggatatga cgttgcagtt caagtagatg gagatggtca
     1021 gcacaatcca tgctatttgg aaaaaatggt tgaggtatta gttcaatctt cagtaaatat
     1081 ggtaattgga tcacgattta tcacaaaaga agggtttcag tcatcatttg ctcgacgtat
     1141 cggcataaag tattttactt ggcttattgc gctactaaca ggaaaaaaaa taacagatgc
     1201 aacgtcaggt ctaaggttaa ttgaccgttc attgattgaa cgttttgcta atcattatcc
     1261 agatgattat cctgaacctg aaacagttgt tgatgtattg gttagtcatt ttaaagtgaa
     1321 agaaattcct gttgtaatga atgagcgaca aggcggtgtg tcatctatct cgttgacgaa
     1381 atcggtctat tatatgatta aagtgacttt agctatttta gttgttagat tgaaaggaaa
     1441 tcgttaaaat gacggtaaca gccagtttat ttataagttt tattgtatta acctttgttt
     1501 tctttttgat taacttaa
//
LOCUS       DATUYR010000129         1460 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.129, whole genome shotgun sequence.
ACCESSION   DATUYR010000129 DATUYR010000000
VERSION     DATUYR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.129"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(164..1285)
                     /gene="mnmA"
                     /locus_tag="VCI33_001697"
     CDS             complement(164..1285)
                     /gene="mnmA"
                     /locus_tag="VCI33_001697"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEP2832588.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVAVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          454 a          313 c          239 g          454 t
ORIGIN      
        1 ctcaactttt acgaactatt tttaaaaata atgtgattat atttaaaata tacagatatc
       61 ataaattaat ccattaacgc atgattggtt tttataaata atatcatatg atgtataagg
      121 aattgttgaa tgtccctttc tttttatctt aataaaagat tcattaaatg tactgacaag
      181 gctgcccatt tttataagcc atgtcaatca taccaccacc aaggcattct tttccgtcat
      241 agaaaacaac agcttgacca ggagtaatcg cgcgctgtgg ctctgcaaaa acaacttctg
      301 ccttatctcc tcgaacatgc accgccacgt gtgaatcggg ttgacgatag cgaaatttag
      361 cagtacattc aaatgtgaac tcttctggca tctcacgtgt aaaatggatg actgaggcgt
      421 caagactatt tgacattagg gcttcatggt agaaaccttg accaacgtat aaaatatttt
      481 gcgacaaatc tttaccgaca acaaaccaag gttgattatc cccaccatgc tgaccaccaa
      541 tgcccagacc accacgctga ccaatagtat aatacataag accggcatgt tctcccatat
      601 cgcgaccatc aatagtcatc atgcgacctt tttgtgccgg taagtactgg ctcaaaaact
      661 gtttgaaatt cttttcacca ataaaacaaa tacctgttga atctttcttt ttagccgttg
      721 caagtcctgc tcgctcagcg atttctctaa cttctgactt ttgcaaatga ccaaggggga
      781 ataaagtctt ttgtagttgt tcctgtgata gttgacttaa aaagtaggtt tggtctttgc
      841 cattatcagc tccacgtaac atgtgaaccg taccattttc atcacgcttg acttgagcat
      901 agtgtcccgt tgctacataa tctgcaccta aagtcatggc atagtcaagg aaagctttaa
      961 attttatttc cttattacac atgacatcgg gatttggagt acgacctgct cggtattcgg
     1021 ccaagaaata ttcaaatact ctgtcccaat attctttttc aaaattgact gagtagtaag
     1081 gaataccgat ttgatcagca acggctgcta catctttgta atcttcagta gctgtgcaaa
     1141 caccaaattc atctgtatcg tcccagtttt tcatgaagac accaataaca tcataacctt
     1201 gctcctttaa aagtagagct gttacagatg aatcaacgcc accactcata ccaacgacaa
     1261 cacgtatctt tgagttatct gtcataaaaa attctcccat caataataag atagaaaaag
     1321 ataatcatca tattatgaat attatgattc tttttcttat cagttaacgt acgattgaag
     1381 gtcgtcgttt tcaaaagtcg atttgaaggt cgattttgaa gcgccatcag cgcaaacctt
     1441 attataacaa ttactgcgtg
//
LOCUS       DATUYR010000130         1447 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.130, whole genome shotgun sequence.
ACCESSION   DATUYR010000130 DATUYR010000000
VERSION     DATUYR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.130"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            85..1155
                     /gene="xerS"
                     /locus_tag="VCI33_001698"
     CDS             85..1155
                     /gene="xerS"
                     /locus_tag="VCI33_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEP2832589.1"
                     /translation="MRRELLLEKIETYKAVMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT          463 a          233 c          279 g          472 t
ORIGIN      
        1 gagaatgaat ggcataaaat acatgagaca atttgcgaaa attcattttt gaaaaaattg
       61 ttattgttaa ggaattatga tgatatgaga cgcgaattat tactagaaaa aattgaaacc
      121 tacaaggctg tcatgccttg gtatgtttta gattattacc aatccaaatt agctgttcca
      181 tacagcttta ccaccttata tgagtattta aaagaataca aacgcttttt tgattggctg
      241 atggatgctg atttaacgca ggcgccaaag attgctgata ttgacttgag cacgcttgag
      301 caccttacca agaaggattt agaggccttt gtgctttatt tgcgggaacg accttctctc
      361 aacacctatt ccaccaaaga ggggctttct caaaccacta ttaatcggac cttgtctgcc
      421 ttatcaagcc tttacaagta cttgactgaa gaggttgaaa atgaccaagg ggagccttat
      481 ttttatcgga atgttatgaa aaaagtgtca acaaaaaaga aaaaagaaac cctggcctcc
      541 agagcggaaa atattaagca gaaactgttt ttaggtgatg aaactctagc tttcttggat
      601 tatgtggata aggaatatga gcaaaaattg tctaatcgtg ccaaatcttc tttccgtaaa
      661 aataaggaga gggatttggc tattattgct ctactacttg cttctggtgt tcgtttgtct
      721 gaagcagtga atcttgattt aaaagatgtc aatctaaata tgatgattat cgaagttatt
      781 cgcaaaggcg gaaaacgtga ttcagtcaat gtagcaggtt ttgcgaaagg ttatttggag
      841 tcttatttag ctgttcgtca gagacgttac aaggctgaaa aacaggatct tgctttcttt
      901 ttaacagaat accgtggcgt tccaaatcga atggatgctt ctagtataga aaagatggtt
      961 ggcaagtact ctgaggattt taaaattcgt gtgactcctc acaaattgcg tcatacgcta
     1021 gcgacaagac tatatgatgc tactaaatct caggtgttag ttagtcatca attaggccat
     1081 tcctctactc aagtcacgga tctttatacc catattgtca atgatgagca aaaaaatgct
     1141 ttagataacc tttaatgtta cataattgtt tttatgtaac attaaagata aaaatagctt
     1201 ttaaattaac tttaatagat gactactatt aaccgtgaca gtttggtgtc aggccgtttt
     1261 ccaaactact caagccactt agaaaaagga atattactgc taactttgtc ccttctttga
     1321 atgacttttt tgtgatgttt atagattaaa tctagtaggg ttctcatcaa aaagatgaga
     1381 taagcctgtt tttagtatgt cagtactttt atttagtatg ataatacgtt attttgtaaa
     1441 gtagctt
//
LOCUS       DATUYR010000131         1440 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.131, whole genome shotgun sequence.
ACCESSION   DATUYR010000131 DATUYR010000000
VERSION     DATUYR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.131"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            56..1279
                     /locus_tag="VCI33_001699"
     CDS             56..1279
                     /locus_tag="VCI33_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP2832590.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLNLINQDITRTQAHQKKRLKKL
                     EDQKASFERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
BASE COUNT          504 a          245 c          254 g          437 t
ORIGIN      
        1 gtgaagatac ttagtaagta catctgctaa gattacgaat agatggggac aataaatgac
       61 gaatttaacg ttttatgcaa aaatcggtat ttcagaagaa gagcatgact cttttgttaa
      121 agaacatcag caaattagcg ttttacaagg tagtgattgg gcaaaaatca aaaatcaatg
      181 gcagaatgag cgaattggta tctataaaga ggaaaagcag gttgcctctt tatcactttt
      241 gattaagcta ttgccacttg gaagaagcat tatttatatt ccaagagggc cagtcatgga
      301 ttatcttgac cgtgatttgg tggcatttac catgaaaaca ctaaaggatt atggtaaaac
      361 taaaaaggcc ctctttatca aatatgatcc agctatcctg ttaaaacaat acgcactggg
      421 acaggaagaa gaagaaaaac ctttagcttt agcagctatt aagaatctcc aagaagctgg
      481 tgttcattgg actggtttaa caatggagat tgcagatagt atccaacctc gtttccaagc
      541 taatatttac actcaagaaa accttgagat gcaatttcct aagcatacca gacgtttaat
      601 aaaagatgct aagcagcgtg gtgtagaaac atatcgtgcc agtcaatcag aacttcacaa
      661 attttccaag attgtctcct taacagaaaa acgtaaaaat atttctttgc gtaacgaagc
      721 ttactttcaa aagttgatga ctacttatgg ggataaggcc tacttacatc tagcaaaagt
      781 gaatattcct caaaaactag atcaataccg ccagcaatta aatcttatta accaagatat
      841 tactcgcacc caagctcatc aaaagaagcg tttaaaaaaa ttagaagatc aaaaagcttc
      901 ttttgaacgt tatataactg aatttgaagg ctttacagac caatatcctg aggaagttgt
      961 tgtagcaggt atattatcta tttcttatgg aaatgttatg gaaatgcttt atgctgggat
     1021 gaatgatgat tttaagaagt tttatcctca gtatctgctg tatcctaaag tttttcagga
     1081 tgcttatcaa gatggtatta tttgggctaa catgggagga gtagaaggct cgcttgatga
     1141 tggacttacc aaatttaagg ccaactttgc tccgacaata gaagaattta taggagaatt
     1201 taatctccct gtcagcccac tttatcatat tgctaatacc atgtacaaaa tacgaaaaca
     1261 gttaaagaat aaacattaaa agactcgcca aaatgagcga gtcttttaat gtttattctg
     1321 ttattcccat tcttcttttc caaacgtacg atatatccca aaccattcaa aatactagat
     1381 tctatttttt ctagtatcac taaatccatc taattatagg acgttttcaa atttttagtc
//
LOCUS       DATUYR010000132         1438 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.132, whole genome shotgun sequence.
ACCESSION   DATUYR010000132 DATUYR010000000
VERSION     DATUYR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.132"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            159..893
                     /locus_tag="VCI33_001700"
     CDS             159..893
                     /locus_tag="VCI33_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEP2832591.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSGFFIEDNDVLCIA
                     SKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIKGLDYKWSENTSIKEALNE"
     gene            886..>1438
                     /locus_tag="VCI33_001701"
     CDS             886..>1438
                     /locus_tag="VCI33_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEP2832592.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVIS"
BASE COUNT          534 a          177 c          291 g          436 t
ORIGIN      
        1 aggaattatc gattataaaa caatcattca tgaaatccat gaacatttta tagacttaat
       61 ttttagttta gaagtacatg aaaaagaaat agacaaaaag gcagaagtag tgaggaagtc
      121 ttatctgaca ctaaatgagt atattaatgg aggctaaaat gaatttgagt atatttggtt
      181 taaagaatat tccttatttg aaagaagggg atagtataga aaagcttatt gaagaaagca
      241 ttaaaacttc aggatttttt atcgaagaca atgatgtgct ttgcatagct tctaaagttg
      301 tatctatcgc agaaggccag gtgatgtctt taaacgagat acaagtgagt gatgtagcaa
      361 aagaaatcca caggaatatt cctcgtaaag atcctagaat aattgaaatt atgctaaatt
      421 tagtcaatag agatctgtca cgtttagata tcaaaaaaaa ttacattggt tgtcgcttgg
      481 aaaatggttt aaagttgaca agtggtggaa tcgatagaaa atcagtagat gaagtatttc
      541 ttttacctaa taatccggat gcttctgcaa aaagaatttc ggaatatctt aaaaaatcac
      601 ttggaaaaaa tgttgcagtt gttataacag atagtgatgg tagagaggat aaaagaggtg
      661 ctactcaagt agcaattggt atttatggta ttcatccttt gagaaaaaca gaagtaatag
      721 attcacaagg agagacaata aaattccaag aagagacttt gtgtgatatg attgcagctt
      781 gtgctggctt agtaatgggg caacgtggaa ctggaattcc agctgtcctc attaagggct
      841 tggattataa atggtctgaa aatactagta ttaaggaagc gttaaatgag tagaaatcag
      901 gactgtaaga atattctaat cattggtttt ggaaaaattg ggaaaattaa agcgtacaag
      961 tggctatcac gcggatatga ggtatatgtc aaagatttga aattctccag catgaaatac
     1021 cttaatacaa atagtgatgt gttggatgat ttatcacgac aatattttac tattgagatt
     1081 tgtactccga caaatcacca ccttacgcta ttgaaaaccg ttataacaag gtatgtctat
     1141 tcttatatta gcattgaaaa accactgtgt aacaggttag aagatttaga agaaatgaag
     1201 tcgctagtag aaagtcaccc acatctagca aaaaaaatgt ttgccagtga acaatacttc
     1261 ttttcgaaaa ttttggaaaa actgttacaa gtagattgtt tctctctaga taaaatcaag
     1321 gaaataacag ttaatttttc taaagatagg attgaggata acgagaatgg gagattttta
     1381 gataaagaga tattgggcta tggaatagag attcctcata tcattgctgt catttctt
//
LOCUS       DATUYR010000133         1411 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.133, whole genome shotgun sequence.
ACCESSION   DATUYR010000133 DATUYR010000000
VERSION     DATUYR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.133"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            281..430
                     /locus_tag="VCI33_001702"
     CDS             281..430
                     /locus_tag="VCI33_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832593.1"
                     /translation="MRERNEKFIGVLKDMHMQILRDFTNEEDPLTRCELAKGFLEIGD
                     YLSKQ"
     gene            complement(416..631)
                     /locus_tag="VCI33_001703"
     CDS             complement(416..631)
                     /locus_tag="VCI33_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832594.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSVEAIKKHNLPF
                     VEAEKQEQINNGKVIAEALERGESLFG"
     gene            690..848
                     /locus_tag="VCI33_001704"
     CDS             690..848
                     /locus_tag="VCI33_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832595.1"
                     /translation="MNWKKLMFGDLEYTFASRDGKEKTSIEFEGGLLPALLVLGGITW
                     LAAWFITK"
     gene            complement(878..>1411)
                     /locus_tag="VCI33_001705"
     CDS             complement(878..>1411)
                     /locus_tag="VCI33_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP2832596.1"
                     /translation="IFISNWLGYRSQIRKLKFDEEKEIYLTLYVPLIKWMNSQSFNNK
                     SYYWLVAFPRYTTNAQDFLTGLLLKNFEKLPVSVAMRYSEYTLNSATSLHFYRNTEYD
                     YDYETFAKKASELFDLIIEQLLTEGTILSQKLSLPNLSKSTLENFLADKKNYIGPRFL
                     SLETHNKPLRPERPLPF"
BASE COUNT          456 a          218 c          294 g          443 t
ORIGIN      
        1 aaaatagttg acaaaatatc tcgttttgag atatactata atcaagctta agggattaag
       61 caaaaatgaa agtaggtaca gctgatgaag tctaggctaa acaaaaagcc taaacacaaa
      121 gaactagaag tcgaaatcaa gattctttgg tttaagctta agattcacta ctcaatagag
      181 tggtgaggat aattagaggg ctaagaagcc ctctccccct aacggggtaa gtttagttta
      241 gcacattggc tgtatctccg caagaatgaa aggagaggtt atgagagaac gaaatgaaaa
      301 gttcatcgga gtgctaaaag atatgcatat gcaaattttg cgagacttca caaacgaaga
      361 agacccctta acaaggtgcg agcttgctaa agggttcctc gaaattggtg actatttatc
      421 caaacaatga ctcgccacgc tcaagagctt cggctataac tttaccatta ttgatttgtt
      481 cctgcttttc ggcttcaacg aaaggtaaat tatgtttctt aatagcttcc acagagtctt
      541 cgtaaagttt gattttatct tcgatagaca aagtagggct agaagaagcg acaatagcga
      601 gagctaaatc ttttgaatta gaaattttca ttagcttatc ctcctttccg ctaggataag
      661 ttgattataa catttttagg aggcacaaaa tgaattggaa aaaactaatg tttggcgatt
      721 tagaatacac gtttgccagt cgtgacggca aagaaaaaac aagcatcgaa tttgaaggtg
      781 gcttattgcc agcgctgttg gtgttaggtg gtatcacttg gttagcagct tggtttatta
      841 caaaataaaa agcccacaag gggcggaaag gggggagtta aaatggcagt ggtctttcag
      901 gtcttagggg tttgttatgg gtttctagtg ataaaaatct gggaccgata tagtttttct
      961 tgtccgccaa aaagttctct aaagtggatt tggataagtt tggtaagctt agcttttggg
     1021 ataatattgt cccttctgtt agcaattgtt caataatgag atcaaataat tcagatgctt
     1081 ttttggcaaa tgtttcataa tcataatcgt attcggtgtt acgataaaaa tgtaaagagg
     1141 ttgcagaatt tagggtgtat tcagagtatc tcatagcaac tgatactggt aatttttcaa
     1201 aattttttaa caacaagcct gtcagaaaat cttgtgcatt tgttgtatat ctagggaacg
     1261 ccactagcca ataataactt ttgttattga atgattgcga attcatccat tttattaacg
     1321 gtacatagag ggttaggtat atttcttttt cttcatcaaa ttttaatttc ctgatttgac
     1381 ttctataacc aagccaattg cttataaaaa t
//
LOCUS       DATUYR010000134         1400 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.134, whole genome shotgun sequence.
ACCESSION   DATUYR010000134 DATUYR010000000
VERSION     DATUYR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.134"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            24..>1400
                     /locus_tag="VCI33_001706"
     CDS             24..>1400
                     /locus_tag="VCI33_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP2832597.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHS"
BASE COUNT          409 a          216 c          250 g          525 t
ORIGIN      
        1 catttactat agaaggtgat aacatgacta tttttcgtaa aaaaaagaaa tattccaata
       61 aaacagaaat gcaaaggcac ttcaaagtta ttgatttagt ttttttaggc ctaggttcaa
      121 tggttggaac aggtatcttt acagttactg gcataggcgc tgctaagtat gcaggtcctg
      181 ctttaacaat atcgattatc atttccgcga ttgctattgg aattttagcc ttattttatg
      241 cagaatttgc gtctcgtatg ccttctaacg gtggtgctta tagttatgtt tatgcaacct
      301 tgggagagtt tccagcatgg cttgttggct ggtatattat tatggaattt ttaacagcta
      361 tatctagtgt tgctgttggc tggggaagtt atttaaaagg attacttgcc aattataata
      421 tagtattacc aaacgcttta aatggcacgt tcaatcttaa gaatggaact tacatagata
      481 ttcttcctgt tttagtgatg tttttcgtga cagggattgt cctaatgaat tcgaaattag
      541 ccctgagatt caatagtttt ttagttattt taaaattttc agcactggct ttatttatct
      601 ttgtaggcat tttttttatt gatcacaata attggtctca ttttgctcca tatggtgtag
      661 gacaaattac tggaggtaaa acaggtatct ttgctggtgc ttctgttatg ttctttgcat
      721 ttttaggatt tgaatctatt tcaatggcag ttgatgaagt gaaagaacca caaaaaacaa
      781 ttcctaaagg tatcatttta tctttgatta ttgtaacagc actctatata gttgtcacca
      841 ctattttaac gggaattgta cactacacta aattaaatgt tccagacgcg gtggcttttg
      901 ctttaagaaa tattagactt tattgggcag ctgactatgt gtcgatagtt gctattttga
      961 ctcttattac ggtatgtatt tctatgacct atgcattagc tagaacaatt tacagtatta
     1021 gtagagatgg tttattacct aaatcacttt atactctcac taaaaaaaat aaagtgcctc
     1081 aaaatgctac tcttgtcact ggactacttg ctatgatttg tgcgggaata tttcctttat
     1141 cgagtctagc agagtttgtt aatatttgta ctttagcata tttaattatt ctgtcgggtg
     1201 caattattaa actcagaaga atagaaggag aaccaaaagc aaatgaattt aaaactcctc
     1261 tagtaccatt cctaccaatg ttggcaatta tcatttgtct atcatttatg agccaatata
     1321 aagcttttac ttggattgct tttgctattg ccacaattat tgggacatta atctacttag
     1381 cttatggtta tactcattca
//
LOCUS       DATUYR010000135         1382 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.135, whole genome shotgun sequence.
ACCESSION   DATUYR010000135 DATUYR010000000
VERSION     DATUYR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.135"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(112..726)
                     /locus_tag="VCI33_001707"
     CDS             complement(112..726)
                     /locus_tag="VCI33_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEP2832598.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            818..1165
                     /gene="ssrA"
                     /locus_tag="VCI33_001708"
     tmRNA           818..1165
                     /gene="ssrA"
                     /locus_tag="VCI33_001708"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          482 a          256 c          214 g          430 t
ORIGIN      
        1 tttaaactat gacacactat agtttaaaag aaagtctttt tcagtgttca tagtaaataa
       61 aaaaaccgtc ttccatcaaa tagaagcggt ttatcaaatt aacaccaaac cttaatgctg
      121 taagaaccaa gatataacat cttttccaaa aataaataaa taactgtagc cgataataga
      181 aattggttta gttattaaaa gaatagttac aaagcgcttt aaagacatgt ccgtcaatcc
      241 cgtaatcata accataatat cagctggaga aacaggcgat gccatacaaa aaataaataa
      301 tttttcatat cctggtgtct ccaaacgcct ttcatacttg taaaatgttt tgtcatttac
      361 aaagagtaag ataaatttac gaccataagt cttaacaagc aagaagagga ctatactgcc
      421 aataatgatg cctacataat tataaataaa acctgttaca gggccaaaga ttaaaaaacc
      481 tgcaactgtc gtaaggcctc caggaatcac aggaaaaaca atctgtatga tctgcacgac
      541 aataaaaaca aacggccccc ataatctata gcgctgcact aagtctttaa gagcattact
      601 atcgtttaat atacctagtc tatacagcca aaaagcgagg aaaaaagtac caattagtgc
      661 aataattcct aaaattttga taattttttg ccataatata taggcttttg aattacgttt
      721 tgtcatagac taatcatacc ataagctgta aaattttgct atttaaaaaa agttttgata
      781 gaatagttag tgtaagaaat tggttaatta tctatttggg gttgttacgg attcgacagg
      841 cattatgagg catgttttgc gtcccatcgg cagatgtaaa ttgccagtta aatataactg
      901 caaaaaatac aaactcttac gctttagctg cctaaaaacc agctagcgtg acttctacaa
      961 gattgcttgt gtcctgttag aagtctcaaa atagcaagct acggttacga aattgtctag
     1021 tttcgtgaca agagattgat agactcgcaa actaatggct tgagttatgt gtctttagtt
     1081 tgttaaatga agacataacc tatggacgta gacaaatatg ttggcaggtg tttggacgtg
     1141 ggttcgactc ccaccagctc catcaatgct taccgtaagt aatcataact tactaaaacc
     1201 ttgttacatc aaggtttttt ctttttgtct tgttcatgag ttaccataac tttctatatt
     1261 attgacaact aaattgacaa ctcttcaatt atttttctgt ctactcaaag ttttcttcat
     1321 ttgatatagt ctaattccac catcacttct tccactacct cctcggtcac aacctcccat
     1381 gg
//
LOCUS       DATUYR010000136         1184 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.136, whole genome shotgun sequence.
ACCESSION   DATUYR010000136 DATUYR010000000
VERSION     DATUYR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.136"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            44..478
                     /locus_tag="VCI33_001709"
     CDS             44..478
                     /locus_tag="VCI33_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP2832599.1"
                     /translation="MIDVSKKLKQLRIDRGIAKSRLITYLSITLRTYDRYEDGSRTPD
                     LVTLMKLSDFYQCSLDELVKGNTNDTLVDRALIVSYRDLKDKGLNDKLAHGLVKQVYD
                     TYEFEKIPQISDANRIKFIYKEDLVTLLESIVSKIQEEKKCD"
     gene            636..1076
                     /locus_tag="VCI33_001710"
     CDS             636..1076
                     /locus_tag="VCI33_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP2832600.1"
                     /translation="MEHRLSMHYFQVKKEIFGVLLLSPVILLIMWGYVSSNQDDGRLT
                     QKQYYETVVNQTVNLVFYKKSCPYCKVAKKEIATQAKDSQVITYYVDTSTGEGQKLVK
                     KYHVKYAPTIVSIRKGNIQSFLYARDRGNNIIVEKEKIKEVFSQ"
BASE COUNT          435 a          136 c          233 g          380 t
ORIGIN      
        1 ttttaaaggt aaaaatttag aatgattgaa agggagctga ataatgattg atgtatcaaa
       61 aaaactaaaa caactaagaa ttgatagagg gattgctaaa tcgcggttga tcacttattt
      121 atcgataact ttaagaactt atgacagata tgaagatggt agtcgtactc ctgatttagt
      181 aacgttaatg aaattatcgg atttttatca atgctcgctt gatgaattag ttaaagggaa
      241 cacaaatgat acgcttgttg atagggcatt gattgtttcg tatagagatc ttaaagataa
      301 gggtttaaat gataaattgg ctcatggtct agtgaagcag gtctatgata cttatgaatt
      361 tgagaagatt cctcaaatat ctgatgctaa tagaattaag tttatctata aagaagattt
      421 ggttacttta cttgaaagta tcgtgtcaaa aatacaagag gagaagaaat gtgactagct
      481 aaaagatttt ggaggtaatg aatgttagtt tatgaattta ttaaacaaaa gattttgtgt
      541 catcagctaa ccaggttgtt acgaataggt actgtaatga taactggact ctcctatagt
      601 ttagataact ggaaaaagtg gagggggatt gaaatatgga gcatcgattg agtatgcact
      661 attttcaagt gaaaaaagaa atttttgggg tattattatt gagtcccgtt attttattga
      721 ttatgtgggg ctatgtttct agcaatcaag atgatggtcg actaacgcaa aagcaatact
      781 atgaaacagt ggtcaaccaa acggttaatt tagtgtttta taaaaagtca tgtccctatt
      841 gcaaagtagc taaaaaagaa attgctactc aagctaagga tagtcaagtt attacttatt
      901 atgttgatac tagtacggga gaaggacaaa aattggttaa aaaataccat gttaagtacg
      961 cacctacaat agtatcaatt agaaaaggaa atattcaatc atttttgtat gctcgagata
     1021 gaggcaacaa cattatagta gaaaaagaaa agataaaaga agtatttagt cagtaagcta
     1081 tgactttatg tgtcatagct tttttatatt tgttgaaaac aagggtaaaa tacagtaaat
     1141 ctccattctt cattctccat ttttaaaccg aaaagatttt actc
//
LOCUS       DATUYR010000137         1158 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.137, whole genome shotgun sequence.
ACCESSION   DATUYR010000137 DATUYR010000000
VERSION     DATUYR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.137"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(53..967)
                     /gene="hasC"
                     /locus_tag="VCI33_001711"
     CDS             complement(53..967)
                     /gene="hasC"
                     /locus_tag="VCI33_001711"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HEP2832601.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     DILERQTPGAGNEVQLTDAIDTLNKTQRVFARKFKGNRYDVGDKFGFMKTSIDYALEH
                     PEVKEDLKNYIIKLGKALEKSKVPTHSK"
BASE COUNT          317 a          228 c          232 g          381 t
ORIGIN      
        1 agatataaac ttaagttgct ataaacaagg aaaaaagtaa agtaccaaca cattactttg
       61 aatgtgttgg tactttactt ttttccaaag cttttcctag tttgataatg taatttttca
      121 agtcctcttt gacctctggg tgttctaagg catagtcgat agatgttttc atgaatccaa
      181 atttatcccc aacatcgtaa cgattacctt taaattttcg tgcaaagaca cgctgagttt
      241 tattgagggt atcgatagca tctgtgagtt gcacttcgtt acctgctcca ggggtctgtc
      301 tttccaaaat atcaaaaatt tcaggggtta ggaggtaacg accaataata gccaaatcac
      361 taggcgcatc ttctggttgt ggtttttcaa caaaggtgtc tacactgtaa aggcccttga
      421 cagccttgcc ttgaggagcg ataaccccat agctagacac atcttcatga ggaactttca
      481 tcacagcgat agtggatgca tgcgtcttgt catagtcctc catgagttgt ttggtgagag
      541 gtttagcgga tgcatttgta atgtccatta agtcatctcc aagcatgacc acaaagggtt
      601 cattgcccac aaaggctttg gcttgtaaga cagcatctcc cagtcctctt gggtggcttt
      661 gacggataaa atgaaggtta atggcagtgg tttcatccac taatttcaac agttcatttt
      721 tccccttagc ttggaggttg tattctaatt caaagtttga atcaaaatgg tcctcgatag
      781 agcgtttagc ttttccggtc accacaagga tttcctcgat gccagatttt agcgcttctt
      841 cgacgataaa ctggatggtt ggtttatcaa cgatgggcaa catctctttg gcaagagctt
      901 tggtagcagg taaaaaacgt gttcctagac ctgcagcagg aataatggct tttctgactt
      961 tggtcatcag taactcctca ctttttataa tctatggctt agtgacattc gttttcagaa
     1021 cggaattcat ttgacatcat gttacgtttt catatgattt ttcgactatt tcatagtgct
     1081 caatcatacc accaactgca gttcttttaa agtaaaaatg aatattattg ttatagatga
     1141 agacatggct agtctcta
//
LOCUS       DATUYR010000138         1135 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.138, whole genome shotgun sequence.
ACCESSION   DATUYR010000138 DATUYR010000000
VERSION     DATUYR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.138"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            81..>1135
                     /locus_tag="VCI33_001712"
     CDS             81..>1135
                     /locus_tag="VCI33_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP2832602.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSML"
BASE COUNT          403 a          176 c          184 g          372 t
ORIGIN      
        1 gtcaaaaaaa gttatcactc acctaacctg aataaaaatg gtataatcgt aaggatcaca
       61 ctatttatta aggaaacatt atgaaaattg ttcagggagt acaacttcat ctgattaaga
      121 ccaaacagtt taaaaccaat catattacct ttcgtttttc aggtgatttg aaccaaaaaa
      181 cagtagcaaa aagagtctta gtggcacaga tgttagcaac agctaatgag tgttatccaa
      241 ctgttaggca gtttagagaa aaattagcac gactttatgg agctagcttg tcaactaacg
      301 tgttaacaaa agggttagtt catattgtgg atatcgatat tacttttatt caagatagat
      361 atgcttgcaa tggggaaaaa atactagatg aaatgattca gtttttaaaa gatatcttgt
      421 tctcacccct attatccatc gcccagtatc agcctaaagt ttttgagact gaaaaaaata
      481 atttgattaa ctacatcgag tcagatagag aggattcttt ttactacagc tctcttaagg
      541 taaaagaatt gttttattgt aataagaatc tacaaatgtc agaatatggt agcccagaac
      601 taattgcaaa agagactgcc tatactagct atcaagaatt tcataaaatg ctcaatgaag
      661 atcagattga tattttcatt ttaggagatt ttgatgatta tcgcgttgtt cagctaattc
      721 atcaatttcc acttgataac cgaaataaaa acctaaattt ctttcattta caaaattctg
      781 tcaatattat taaagaatct attgaaaaaa gagcagttca tcaatctatt ttacaattag
      841 cttatcactt cccttcagtt tttggacaac gtgactatta tgctttagtt cttttaaatg
      901 ggttgttagg ctcttttgca cattcccgtc tctttataaa aattagagaa gaagaaggac
      961 ttgcttatag tatagggtgt cgttttgata gttatacagg tttatttgaa atttatactg
     1021 gaattgacag tcaacatcgc actaaaacgt tacaactaat catccaagaa ttaaatgcca
     1081 ttaaaatggg acgattctca gaacaattga ttaaaaagac cagatcgatg ttgtt
//
LOCUS       DATUYR010000139          978 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.139, whole genome shotgun sequence.
ACCESSION   DATUYR010000139 DATUYR010000000
VERSION     DATUYR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.139"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            126..407
                     /locus_tag="VCI33_001713"
     CDS             126..407
                     /locus_tag="VCI33_001713"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP2832603.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(884..971)
                     /locus_tag="VCI33_001714"
     tRNA            complement(884..971)
                     /locus_tag="VCI33_001714"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(935..937),aa:Ser,seq:gct)
BASE COUNT          338 a          206 c          115 g          319 t
ORIGIN      
        1 agaatgaatt tttagttact tatccacagg ttaataactt cttttcgcct aaaatattat
       61 ccacaatagt gacattttct attatcagtt atagtataat ttcattagga atctaaaacc
      121 ttagcatcac ggttttaaat tatgtaaatg ctggggacgc aaaaagcatt cataatcatc
      181 tctttcatta taccagacag gtgagaaggc atgtcaaagt tattaccata ggcatactca
      241 gtgcccacta ctatgagatt actatgcttt ttctacaggc taaaattatc ctcgattgtt
      301 ttcatatccc ccaacgtctt agacaagcta taactcgctt tcgtatccga atcataaata
      361 cttctgctat aaaacctatc gcgtatagaa ctctcaaact ttactgaaaa ctcatacagc
      421 atgattcacg gaagctcatc aataatagct ttattgccca atttttcgta cgcccataac
      481 taacatctag aaaagaaaag ctcttactca caagatctct gaatttcaat aattattcct
      541 aactctatca actcttgctc ttcccatttt tcagacaatt tttaaaattt ttctaaaaga
      601 aaagaaaaca agattagcaa tatacttaaa ctcaactact ctaaggccaa attagaaaca
      661 gtcaacaacc ttatcaaagc catcaagtta aacgttttac tcctctacag tagtcaaaga
      721 gcctatcatt ttagttatcg aaataatgaa cgatttaaat attctattta gttggttttg
      781 aagattacaa aaaaaagacg ataaccgtct tctttccccc ttatttataa ttcatccttt
      841 aatatgtttc atttaacata ttaaccaaaa tatctttaca aaacggagaa taagggattc
      901 gaacccttgc gccagttacc cgacctaacg atttagcaaa ccgtcctctt cagcctcttg
      961 agtaattctc caaatata
//
LOCUS       DATUYR010000140          967 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.140, whole genome shotgun sequence.
ACCESSION   DATUYR010000140 DATUYR010000000
VERSION     DATUYR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.140"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            124..396
                     /gene="rpsP"
                     /locus_tag="VCI33_001715"
     CDS             124..396
                     /gene="rpsP"
                     /locus_tag="VCI33_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEP2832604.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            406..645
                     /locus_tag="VCI33_001716"
     CDS             406..645
                     /locus_tag="VCI33_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEP2832605.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
BASE COUNT          316 a          158 c          170 g          323 t
ORIGIN      
        1 tttaaagaaa gtgtcaagaa aaaaacttga cactttcttt aaactgagtt atactgttta
       61 acataggaat ttagcgtagc tgtatttctt atacattact aaaagaaaag agactattta
      121 aaaatggcag taaaaatccg tttaactcgt atgggttcta agaaaaaacc tttctaccgt
      181 attaatgttg cagattcacg tgcaccacgt gatggtcgtt ttatcgaaac agttggaaca
      241 tacaacccat tagtggctga aaatcaaatt actattaaag aggatcgtgt tcttgagtgg
      301 ttatctaaag gagcgcagcc ttctgatacc gttcgtaaca tcttgtctaa agctggtgtt
      361 atggctaagt tccacgacca aaaattctct aaataataac agcctatgga taccattgaa
      421 aatcttatta tcgctattgt gaaacctttg atttcacaac cagataatct taccattaaa
      481 attgaagata ctcctgactt tttagagtat catcttgact tggatgctca agacatcggt
      541 cgtgttatcg gcaaaaaagg tcgaactatt actgcgataa gatcgattgt ctactcggtt
      601 ccaacactag gaaaaaaagt tcgcttagtt attgatgaga aataatcact aacttgagat
      661 ggattgaaaa gcttgttgac ttaaagtcaa caagcttttt gaatagcaaa aatcttaata
      721 tttctgaaat gaaaagatta gatttttctg tctttaacta aaaagaagtt aaagaatggc
      781 ttagctgatt gcctttataa gagattatac tggtttttga tagctgctgt catgtaaaat
      841 agctacaatt catcttacga gattgacctt agcattgcca gtgcagtctt tattttctaa
      901 cctgctgttt gttgtatgtt tatagtttcc taaaaaatgc tctctcagtt ttttggggga
      961 cattcct
//
LOCUS       DATUYR010000141          956 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.141, whole genome shotgun sequence.
ACCESSION   DATUYR010000141 DATUYR010000000
VERSION     DATUYR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.141"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..746
                     /locus_tag="VCI33_001717"
     CDS             <1..746
                     /locus_tag="VCI33_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832606.1"
                     /translation="FDNVTFGYNGNKVLENLSFKLEEGKKYALVGHSGSGKSTIVKLL
                     SGFYKVDSGSIKIGERPLESYSKDALINTISFVFQDSKLFKTSIYENVALANRKASRE
                     EVMKAMELAGCNVILNKFPERENTIIGSKGVYLSGGEKQRIAIARAMLKKSKIVIMDE
                     ASASIDPDNEYELQKAFKNLMKDKTVIMIAHRMSSIRSVDEILVLENGEIIERGSDHE
                     LISKGGKYKELLELYDSANQWRVKDEEIL"
BASE COUNT          340 a          108 c          193 g          315 t
ORIGIN      
        1 agtttgataa tgtaacattc ggttacaatg gaaataaagt attagaaaat ttatctttta
       61 aattagaaga aggaaaaaaa tatgctttgg tgggtcattc aggatcaggt aaatcgacta
      121 ttgtaaagct attatctggt ttttacaaag tagatagtgg cagtataaaa attggagaga
      181 gacccttaga gtcttatagt aaggatgctc taataaacac aatttctttt gtttttcagg
      241 atagcaaact ttttaagaca tctatttacg aaaatgttgc tcttgctaat agaaaggcaa
      301 gtagagaaga agttatgaag gctatggaac ttgcaggttg taatgttatt ttaaataagt
      361 ttcctgaaag ggagaatacc ataatcggct caaaaggagt ctatttatca ggaggagaaa
      421 aacagagaat tgctattgcg agagccatgc tcaagaaatc taaaattgta attatggatg
      481 aagctagtgc ttctattgat ccagacaatg agtatgagct tcaaaaggcc tttaaaaacc
      541 ttatgaaaga taaaacagtt attatgattg cccataggat gagtagtatt cgttctgttg
      601 atgaaatttt agttttagag aatggagaaa taattgaaag aggcagtgat catgaactta
      661 tttcaaaagg tggaaaatat aaggaattat tagagcttta cgatagtgca aatcaatgga
      721 gggttaaaga tgaggaaatt ttataaaaaa cgcttttgtc tgactgataa aggggctaaa
      781 gatctcacta aatctactat atcaagtttc ttggtctact gtattaatat gattcctgcc
      841 tttattttaa tgatgttaac agatgagctt cttttgaaaa atgttaaaag cccttattta
      901 tattttggcg tttctgttgt tacattagta ataatgtatg ttttgttagc caaaga
//
LOCUS       DATUYR010000142          926 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.142, whole genome shotgun sequence.
ACCESSION   DATUYR010000142 DATUYR010000000
VERSION     DATUYR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.142"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..115)
                     /locus_tag="VCI33_001718"
     CDS             complement(<1..115)
                     /locus_tag="VCI33_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP2832607.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFE"
     gene            complement(184..255)
                     /gene="shp3"
                     /locus_tag="VCI33_001719"
     CDS             complement(184..255)
                     /gene="shp3"
                     /locus_tag="VCI33_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEP2832608.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            335..>926
                     /gene="rgg3"
                     /locus_tag="VCI33_001720"
     CDS             335..>926
                     /gene="rgg3"
                     /locus_tag="VCI33_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEP2832609.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINC"
BASE COUNT          334 a          165 c          134 g          293 t
ORIGIN      
        1 attcaaataa ttcatctgat atagaatgtt caactggatt accaagcaat gcagaataca
       61 taaatttcat cctcctacgt ttaattagtt tactactaac ataaagtaca agcattacac
      121 agagctaacc attacctata agtctttaga gtatctaaag acttaggtgt acacctaagt
      181 aacttaccca ccaacaataa tgataatatc cattgctaaa attaaaatcg gcaaaaattt
      241 tgaaattttc ttcataagcg gacttccttt cagtttttat ttattgtaca ataaaaaaaa
      301 acggaattga tttttgttgg gaaagtggga aaatatgaaa tcaaaacttg gtgccacact
      361 tagaaaagtt cgaaaaggaa agcaaattag tctgtgcttc gtggcagatg aacacctttc
      421 aaaatcacaa atctctcgtt ttgagcgcgg ggagtcagag atatcgtgta tccgactgat
      481 caatattcta gacaaattac acattacttt ggatgagttt cttattttac atgatgaaga
      541 ctataccaat tccgaaacat ttgctaacct ggtacagtat attcgtaagc aatattcatc
      601 acaaaatatc aataatatag catgtttact ttccgacact tctgattaca ctctgaattc
      661 ttttgagaaa acaatggtaa agtccatttt acacacaatg gattcaagca tcattccatc
      721 agatgatgaa ttacttcaac taacagatta tctctttaaa gttgaaaaat ggggctacta
      781 tgaaatcctt ttgttgggta actgtgtacg aacaattaac tacaactcct acttcctatt
      841 gacaaaggag atgttaaata attacattta ctcttcacgg aacaaaacta ataaacgaat
      901 tgtcactcag cttgctataa attgtt
//
LOCUS       DATUYR010000143          912 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.143, whole genome shotgun sequence.
ACCESSION   DATUYR010000143 DATUYR010000000
VERSION     DATUYR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.143"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            26..880
                     /locus_tag="VCI33_001721"
     CDS             26..880
                     /locus_tag="VCI33_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEP2832610.1"
                     /translation="MKSKKVVSVISLTLSLFLVIGCAKVDNNKSVNCKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
BASE COUNT          336 a          140 c          176 g          260 t
ORIGIN      
        1 ttaataagca aaaaggagat tttcaatgaa atccaaaaaa gttgttagtg ttatatcact
       61 taccttatcc ctttttttgg tgataggttg tgctaaagtt gataacaaca aatcagttaa
      121 ttgtaagcct gctactaaac aaacctataa tagctatagt gatgaccaat taagatcgcg
      181 tgaaaatacc atgtctgttt tatggtacca gcgagctgcg gaaactcaag cgctttattt
      241 acaggggtat cagttagcaa cggatcgctt aaaagaacaa ctcaataaac caacggataa
      301 accttattca attgtattag atattgacga aacagttctt gataatagcc cttatcaagc
      361 taaaaatgtt ttggaaggaa caggatttac acctgaaagc tgggattatt gggtacaaaa
      421 gaaagaagca aaaccggttg ctggtgctaa agactttttg caatttgcag accaaaatgg
      481 tgttcaaatt tactacattt cagacagatc aactactcaa gtagatgcta caatggaaaa
      541 tctccaaaaa gaaggtattc cagtacaagg tcgtgatcat cttctattct tagaaaaagg
      601 cgtaaaatca aaggagagtc gtcgtcaaaa ggtcaaagaa acaactaatg taacgatgct
      661 atttggtgat aatcttctag attttgctga tttttcaaaa aaatctcaag aagatagaac
      721 agctttatta tcagatttgc aagaagagtt tggaagacgc tttatcattt tccctaatcc
      781 tatgtatggt tcatgggaag gtgccattta taaaggtgaa aagctggatg tgcttaagca
      841 actagaggaa cgccgtaaaa gtttaaaaag ctttaaataa agtaattgaa aaagaggtgc
      901 aaaagaaaat gt
//
LOCUS       DATUYR010000144          893 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.144, whole genome shotgun sequence.
ACCESSION   DATUYR010000144 DATUYR010000000
VERSION     DATUYR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.144"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..270)
                     /gene="cadX"
                     /locus_tag="VCI33_001722"
     CDS             complement(<1..270)
                     /gene="cadX"
                     /locus_tag="VCI33_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEP2832611.1"
                     /translation="MKKDSICQVGVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRR"
     gene            complement(282..893)
                     /locus_tag="VCI33_001723"
     CDS             complement(282..893)
                     /locus_tag="VCI33_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEP2832612.1"
                     /translation="MQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQFL
                     GSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLSKD
                     NKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKLA
                     QVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
BASE COUNT          311 a          157 c          151 g          274 t
ORIGIN      
        1 tcttctagtg tccaagatgt tttgattggc tagtttacga agatggtggg aggcagatgc
       61 catactgaga tttaataaac aggctatatc gcagacacag agttcttcga cggcaaggag
      121 ataaaagatg atatttatct gtttattatc ggtaaatttt gataaaatac gaagtgattt
      181 ttggactttt tccttttcaa ggtagttcgt tgcggttgta acattttgtt gatttataac
      241 acctacttga cagatactat cttttttcat aattttttct cctagcctaa cacagcccat
      301 agcatgtcaa agctgttgtt ttcaatcagg atatacatcc ccaatcctaa atagacaacg
      361 gcaataaacc atctgctata tttttccaaa gtttctccaa cagaagggac ttgtgccaat
      421 ttttgggcag aaaaaaccaa gagataaatc atgactagaa aggtaagtaa agccactatc
      481 aaattcgcta aatttaaggt agtaaaatat gggacaaaga caccaatatt gtcagcacca
      541 caacttgcaa aagtaatcat agcgactaga aaaatcaggt ttttattatc tttgctcaaa
      601 ccttctttgg caatagcttc tccatcagaa tctcctaaaa gcaaaacttt gaggcctagg
      661 aaaattggaa tcaaaccgag caaacctaaa atctctttac taggaatata atctaagaca
      721 aatgcaaaaa gcaaacttag caatattaga ctaacagagc ctagaaattg tcctaaatag
      781 atgttaatga tgtcttttct gctttttctt ttggcaaaaa ataacattag gataataagt
      841 aagtctacgg ctgtcccaga atacaggatt attgaagtaa caacattttg aat
//
LOCUS       DATUYR010000145          851 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.145, whole genome shotgun sequence.
ACCESSION   DATUYR010000145 DATUYR010000000
VERSION     DATUYR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.145"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>851
                     /locus_tag="VCI33_001724"
     CDS             <1..>851
                     /locus_tag="VCI33_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEP2832613.1"
                     /translation="KTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNAIDPLLLKK
                     KLTETQINASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTK
                     GWSNSFDLDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEHFSNSIVPI
                     ISGDELRSEKNYSIIIYDRLSQATIQAGLDIETAYRARDRFIKETESTISLNEVLKLR
                     DTAILFYTQQVDSLKRHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLR
                     KLFKQEK"
BASE COUNT          308 a          152 c          124 g          267 t
ORIGIN      
        1 gtaaaactca acatgatttt ttatttcatt atggctcttt aggagaactt tttcttgtcc
       61 accatattca acaatattat attatcatcg gtccctggcg tagcaatgct attgatcctt
      121 tacttcttaa gaaaaagctg actgaaacac aaatcaatgc tagtgaacaa gactacttta
      181 ttgatagact atcccaatta cctttcttct cacttagtca aatccgagaa ttactaatag
      241 taaccaatta ttgtctaact ggggttgtga aagataaatt atcagagccc ttacattact
      301 acacgaaagg gtggagcaac tcctttgatc tagataaaat taagcaattt tctaaacaga
      361 atatgagttc ttacaaatat cagtatcatt ttgaaaataa tattctaaaa gctgttaaat
      421 caggtagtga atttcttttg aaagaaacag tggaacactt tagcaattct atcgtaccta
      481 taatcagtgg agatgaattg cgatctgaaa aaaactactc catcattatt tatgatcgtc
      541 tctcacaggc taccatccaa gcaggacttg atatcgaaac agcttatcga gcacgagatc
      601 gttttataaa agaaaccgag tcaactataa gtctaaatga agttttaaaa ttacgtgata
      661 ctgctatctt attctatact caacaagttg attctttaaa aagacatctc gaaacccctc
      721 attctcaaac tattgtcgca gtgattcgat atcttgaaaa taatctaaat cgctttatta
      781 aaacagaaga aatagctaaa gaatgtcaca tgagtgaatc gaagttacga aaactattta
      841 agcaagaaaa a
//
LOCUS       DATUYR010000146          844 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.146, whole genome shotgun sequence.
ACCESSION   DATUYR010000146 DATUYR010000000
VERSION     DATUYR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.146"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            55..813
                     /gene="mf2"
                     /locus_tag="VCI33_001725"
     CDS             55..813
                     /gene="mf2"
                     /locus_tag="VCI33_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEP2832614.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAAKVRTYPNVSHANTH
                     YKNTASSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVTMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTATPKN"
BASE COUNT          291 a          152 c          145 g          256 t
ORIGIN      
        1 gtgcaatatt tgatccactc taaaagctaa aaatggaaag gtttaggtgt tatcatgaaa
       61 ttatctaaac aaaaggcaag tttgcttacc gctgttttac tgttactttc tctttctata
      121 acgacaataa ctgttgacgc agctaaggta cgaacttatc cgaatgtatc ccatgcaaac
      181 acacattata aaaatactgc ttctagtaag ctcttacctt ttactgctaa ttatcagtta
      241 caacttggtg agttggataa ccttaaccgc gctacctttt cacatatcca actgcaagat
      301 agacacgaaa ctaaagatgt acgcactaaa ataaattatg accctgtggg atggcataac
      361 tatcaatttc catacggaga tggctcaaaa tcttcatggg taatgaatcg tgggcatcta
      421 gtcggatatc aattttgtgg attaaacgat gaaccaagaa atttagtgac aatgacagcc
      481 tggctaaaca caggtgctta ttcaggagca aatgacagca atcctgaagg gatgttatac
      541 tatgaaaacc gtttagattc ttggcttgca cttcaccctg acttttggct agattataaa
      601 gtcactccta tatatagtgg caatgaagta gtgcctcgac aaattgaatt acagtatgtt
      661 ggaattgatt catctggtga gttgcttact ataaggctaa atagtaataa agaaagtatt
      721 gatgaaaatg gtgttactac agtaatctta gaaaactctg ctccaaatat caaccttgac
      781 tatttaaatg gaactgccac tcctaaaaac taaaatataa atacatttat aaaatttcta
      841 aata
//
LOCUS       DATUYR010000147          813 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.147, whole genome shotgun sequence.
ACCESSION   DATUYR010000147 DATUYR010000000
VERSION     DATUYR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.147"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>813
                     /locus_tag="VCI33_001726"
     CDS             <1..>813
                     /locus_tag="VCI33_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832615.1"
                     /translation="NLRIDIAETLSKLPLSYFSKHNLSDISQTIMADVEKVEHAMSHS
                     IPKVGGMVLFFPLIGIMLMIGNFKMGLAVMIPTILSFILIPIAKKSQVKGNKKYHKVL
                     RENSEKFQETIELQQEINSFNLSDEVRSSLYMQMDESEKIHLKVEQGSLFVMGLSSAL
                     GFVSIAVVSFVGISLILSGEIDILYLLGYLMAAIKIKDIFDLSKEGLLEVFSIDPSVE
                     RIREIKETKIQEGKDVELNMFDIVFKNVAFSYDDNQVLKDISFTAKQGEVTAL"
BASE COUNT          289 a          106 c          154 g          264 t
ORIGIN      
        1 aatttaagaa tagatattgc tgaaactcta tcaaaacttc ccctatccta cttctcaaaa
       61 cataatttat cagatatatc tcaaacgatt atggcagatg tggaaaaagt agaacacgct
      121 atgagtcatt caattcctaa agtaggagga atggttttat ttttcccact aattggaatt
      181 atgttaatga taggtaattt taagatggga ctagcagtaa tgatcccaac tattttaagt
      241 tttatactaa tacctattgc taaaaagtcg caggtaaaag gaaacaagaa atatcacaag
      301 gtacttagag aaaactcaga aaaattccaa gaaaccattg aacttcaaca agaaataaat
      361 agttttaact tatccgatga agtgaggtct tcgttatata tgcagatgga tgaaagtgaa
      421 aaaatacatc ttaaagttga acaaggctct ctttttgtaa tgggtttgtc ctctgctctt
      481 ggctttgtaa gtattgcagt agttagtttt gttgggatta gccttattct atctggggag
      541 attgacattt tatatctctt aggttattta atggctgcca taaagataaa agatattttc
      601 gatttatcaa aagaaggatt attagaagtg ttctctatag atccaagtgt tgaaaggatt
      661 agggagatta aggaaacaaa aattcaagag ggtaaagatg tagaactgaa tatgtttgat
      721 atagtcttta aaaatgtagc tttttcttat gatgataatc aggttttaaa ggacattagc
      781 tttactgcga aacagggaga ggtaacggct cta
//
LOCUS       DATUYR010000148          780 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.148, whole genome shotgun sequence.
ACCESSION   DATUYR010000148 DATUYR010000000
VERSION     DATUYR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.148"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>780
                     /gene="alp3"
                     /locus_tag="VCI33_001727"
     CDS             <1..>780
                     /gene="alp3"
                     /locus_tag="VCI33_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-like surface protein Alp3"
                     /protein_id="HEP2832616.1"
                     /translation="LRNRIDEVINNANDPKWSDGSRDEVLTGLEKIKKDIDNNPKTQI
                     DIDNKINEVNEIEKLLVVSLPDKIKYSPEAKHRTVEQHAELDAKDSIANTDELPSNST
                     YNWKNGHKPDTSTSGEKDGIVEVHYPDGTVDDVNVKVTVTSKKTDNTAPTLTVTPEQQ
                     TVKVDEDITFTVTVEDENEVELGLDDLKAKYENDIIGARVKIKYLTKEPNKKVMEVTI
                     MKATLADKGAITFTAKDKAGNQAEPKTVTINVLPLKDSNEP"
BASE COUNT          320 a          113 c          151 g          196 t
ORIGIN      
        1 ctttaagaaa taggatagat gaagtaataa ataatgcaaa tgatcctaag tggagtgatg
       61 ggagtcgtga tgaagtctta actggattag aaaaaataaa aaaagatatt gataataatc
      121 caaaaacaca aatagatatt gataataaaa ttaatgaagt caatgaaata gagaaattgt
      181 tagttgtatc gctaccagat aaaattaagt attcgccaga ggctaagcat aggactgttg
      241 aacaacacgc ggaattagat gcaaaagata gcattgcaaa tacagatgaa ttgccatcaa
      301 attcaacgta taactggaaa aatggtcata aaccagacac ctcaacatca ggtgaaaaag
      361 acggaattgt tgaagttcac tatccagatg gtactgttga tgatgtgaat gttaaagtaa
      421 ccgttacatc gaaaaaaact gataatacag ctccaacatt aaccgtcact ccagagcaac
      481 agactgttaa agtggatgaa gatattacct ttacggttac agttgaagac gaaaatgaag
      541 ttgaactagg tttagatgat cttaaagcta agtatgaaaa tgatatcatt ggagctcgtg
      601 ttaaaattaa gtatcttact aaagaaccta ataagaaagt catggaagtg acaattatga
      661 aagctacttt agcagataag ggcgcaatta cctttactgc aaaagataaa gcaggtaatc
      721 aagcagaacc taagacagtt accatcaatg ttcttccgct taaggatagc aacgaaccaa
//
LOCUS       DATUYR010000149          743 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.149, whole genome shotgun sequence.
ACCESSION   DATUYR010000149 DATUYR010000000
VERSION     DATUYR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.149"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>743
                     /locus_tag="VCI33_001728"
     CDS             <1..>743
                     /locus_tag="VCI33_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEP2832617.1"
                     /translation="KEHDVTFFDIDNKKIDLINKRQSPLKEAAINKLLCKAKNINATS
                     SEELAYKDATFIILSLPTNLKFNKLDTSIIEISVSNILKINKKATIVIKSTVPIGFTE
                     YLRNRFHYNDIIFSPEFLREGSTIHDQLYPSRTIVGNESRNSQLFLDILTDISVEKDS
                     PSLLVGSSEAEAIKLFSNAYLAQKIAFFNELDTFAEMQNLDSKKIIEAMGYDQRIGNS
                     HNNPSFGFGGYCLPKDIKQLEYHFKEIPA"
BASE COUNT          270 a          115 c          118 g          240 t
ORIGIN      
        1 aaaggaacac gacgtcacct tttttgatat tgataataaa aaaattgatt taataaataa
       61 aaggcaatcc cctcttaaag aagcagctat aaacaaactt ttatgtaagg caaaaaatat
      121 taatgcaact tcttctgaag aattagcata taaggatgcg actttcataa tcttgtcttt
      181 gccaaccaac ctaaaattta ataagcttga tacttccatt atcgaaattt ctgtaagtaa
      241 tattttaaag ataaacaaaa aggctacaat tgtaataaag tcgacagttc caattggttt
      301 tacagaatat ttaaggaatc gatttcacta caacgatatc attttttcac ctgagttcct
      361 tagggaagga tcaactattc atgatcaatt gtatccttcg agaactatag ttggaaatga
      421 atctagaaat tctcaattat tcttagacat actaacagat atatcggttg aaaaagactc
      481 gccatcttta ttagttggct cttctgaagc agaagcgata aagttatttt cgaatgcata
      541 cttggcacaa aaaattgctt tttttaatga gttggatacg tttgctgaaa tgcaaaattt
      601 ggactcaaaa aaaattattg aggctatggg atatgaccag agaataggaa attcgcacaa
      661 taatccttct ttcggttttg gtgggtactg tcttcctaag gatattaagc aattagagta
      721 tcattttaaa gaaattccag cac
//
LOCUS       DATUYR010000150          731 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.150, whole genome shotgun sequence.
ACCESSION   DATUYR010000150 DATUYR010000000
VERSION     DATUYR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.150"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>731)
                     /locus_tag="VCI33_001729"
     CDS             complement(<1..>731)
                     /locus_tag="VCI33_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP2832618.1"
                     /translation="FDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYSQQLL
                     KQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTGKYAK
                     RIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTALIPV
                     IGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAMWVLM
                     AITIGASLKGIVGMIIAVPLAATLY"
BASE COUNT          281 a          142 c          106 g          202 t
ORIGIN      
        1 gataaagtgt cgctgctaaa ggaactgcaa taatcatacc aactattccc tttaaagaag
       61 cgcctattgt aattgccatt aatacccaca tagccggtaa tccaatcgat ccaccaacta
      121 ctttcggata aataaaatta ccttcaatct gctgcaaaat aattaaaaaa atgataaaaa
      181 taatggcctg tgacatggac tgagtcataa ttaaaataaa tccaatagct gctccaattg
      241 aagcacctat aactggaatc aatgctgtaa aagctactaa aacgccaata gtccctgcaa
      301 atggtaatct taatataaac atgccactag cagtcagcga acctaaaatc atagcttcta
      361 aggtctgact aacaaaaaat ccgtgaaaac gctgatgcaa caactctaac aagtaatgaa
      421 tgcgtttagc gtacttacca gtataagtat ctactaaagt atttccctga cgacaaagat
      481 cttctttact tgctaaaacg tataacgaaa aaacaaaact aataaaaaga ttaataattg
      541 cagaagcaat aaccgtgaca gatgttaaaa tatttgtcaa aacagtaaga aattgcttta
      601 ataactgttg actatagtta gcaatcgtct ctgtaagttt gccatcgcca ccaatatact
      661 gaatagtacg tgctaacagt ttattgtgct caagattgtt aacaacttct tgtatcgtta
      721 tagtatcaaa t
//
LOCUS       DATUYR010000151          722 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.151, whole genome shotgun sequence.
ACCESSION   DATUYR010000151 DATUYR010000000
VERSION     DATUYR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.151"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(3..76)
                     /locus_tag="VCI33_001730"
     tRNA            complement(3..76)
                     /locus_tag="VCI33_001730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Arg,seq:acg)
     gene            complement(93..177)
                     /locus_tag="VCI33_001731"
     tRNA            complement(93..177)
                     /locus_tag="VCI33_001731"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Leu,seq:taa)
     gene            complement(186..257)
                     /locus_tag="VCI33_001732"
     tRNA            complement(186..257)
                     /locus_tag="VCI33_001732"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Gly,seq:gcc)
     gene            complement(270..342)
                     /locus_tag="VCI33_001733"
     tRNA            complement(270..342)
                     /locus_tag="VCI33_001733"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Thr,seq:tgt)
     gene            complement(353..434)
                     /locus_tag="VCI33_001734"
     tRNA            complement(353..434)
                     /locus_tag="VCI33_001734"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Leu,seq:tag)
     gene            complement(440..512)
                     /locus_tag="VCI33_001735"
     tRNA            complement(440..512)
                     /locus_tag="VCI33_001735"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Lys,seq:ttt)
     gene            complement(544..616)
                     /locus_tag="VCI33_001736"
     tRNA            complement(544..616)
                     /locus_tag="VCI33_001736"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Asp,seq:gtc)
     gene            complement(634..706)
                     /locus_tag="VCI33_001737"
     tRNA            complement(634..706)
                     /locus_tag="VCI33_001737"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(671..673),aa:Val,seq:tac)
BASE COUNT          186 a          207 c          159 g          170 t
ORIGIN      
        1 gatgcaccct agaggagtcg aacctctaac cgcctgattc gtagtcaggt actctatcca
       61 gttgagctaa gggtgctatt tcaaaaaatt aatgccgagg accggaatcg aaccggtacg
      121 atgtaaacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca ccccggctcc
      181 tcaaaagcga acgacgggat tcgaacccgc gacccccacc ttggcaaggt gatgttctac
      241 cactgaacta cgttcgcaac taatctcaat gccggctaca tgacttgaac acgcgaccct
      301 ctgattacaa atcagatgct ctaccaactg agctaagccg gctaatcttt tatgcgggtt
      361 aagggacttg aacccccacg ccgttaagcg ccagatccta aatctggtgc gtctgccaat
      421 tccgccaaac ccgcttttat gacccgtact gggctcgaac cagtgaccca ttgattaaaa
      481 gtcaattgct ctaccaactg agctaacgag tctttccttc aaaaaatatg actactcttt
      541 gaacggtccc gacgggaatc gaacccgcga tcttcgccgt gacagggcga cgtgataacc
      601 gctacactac gggacctgta ttttccaata ctatgggagt taacgggatc gaaccgctga
      661 ccctctgctt gtaaggcaga tgctctccca gctgagctaa actcccattg agtgtgtcat
      721 ta
//
LOCUS       DATUYR010000152          704 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.152, whole genome shotgun sequence.
ACCESSION   DATUYR010000152 DATUYR010000000
VERSION     DATUYR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.152"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            51..665
                     /locus_tag="VCI33_001738"
     CDS             51..665
                     /locus_tag="VCI33_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEP2832619.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKDKSKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
BASE COUNT          251 a          120 c          149 g          184 t
ORIGIN      
        1 taataattaa aagatattat ggataacgga gaaaagtgag gtattattga atgtttaaga
       61 aagaaaattt aaaacaacgt tattttaatt ttggattagt agcgttagct ctaacaatat
      121 tagccatcat ttttgccttc tcaagtaaaa atgctgatac taagtcttat gctaagaagt
      181 cagaaagtaa aatggtaaca atcgacaagg ctccaaaaaa taatcatgct attactaaag
      241 aagaaagcaa agataaatca aagagcattg cttcggagcc tattcccaca gtagaaaact
      301 ctgtagctcc gacagtaaca gaggaagcac cggttgttca gcaagaagtg actcaaactg
      361 ttcagcaggt atcttcagta gcctataatc caaacaatgt ggtactttcc aatggaaata
      421 ctgctggtat tgtaggaagt caagcggcgg cacagatggc agcagcaaca ggtgttccac
      481 aatcaacttg ggaacatata attgcgcgtg aatctaatgg aaatcctaac gcagctaatg
      541 cttctggggc atcagggttg ttccagacaa tgccaggttg gggttctaca gcaacggttg
      601 aagatcaagt caatgcagcc ttgaaagcct atagtgcaca aggtttatca gcttggggtt
      661 actaaataaa catagctgga agaaataaaa cctattgtta gtat
//
LOCUS       DATUYR010000153          698 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.153, whole genome shotgun sequence.
ACCESSION   DATUYR010000153 DATUYR010000000
VERSION     DATUYR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.153"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>698)
                     /locus_tag="VCI33_001739"
     CDS             complement(<1..>698)
                     /locus_tag="VCI33_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP2832620.1"
                     /translation="KHNGYIAVFLSALSAFLMVYGYSLLYKLLRSSIVEGDLLVARTL
                     SIQAVLYLTIGALVYFLSNFFSHKLGFRLETNLRKKGIDGLTGASFRFYDLNPSGIIR
                     KTIDDNAAKTHTAVAHMIPDNSQAFLIPVLSLILAFSISSRVGLVILILTIVGALILK
                     NMMGGGEFMEYYQKSLEILSGETVEYVRGMQVVKIFGATVESFKTLYKAIKDYSKYAL
                     DYSISSKKPYVLYQ"
BASE COUNT          248 a          124 c           98 g          228 t
ORIGIN      
        1 attgatatag tacgtaaggt tttttagaac taattgagta atcaagagca tacttagaat
       61 aatccttaat cgccttataa agagttttaa aagactctac agtagctccg aatattttaa
      121 caacttgcat gcctctgaca tattcaactg tttcaccact taaaatttct aaggattttt
      181 ggtagtattc catgaactct cctccaccca tcatgttctt taaaataaga gcccctacta
      241 ttgttagtat aagaataact aatcccactc tgctactaat agaaaaggca agtattaaag
      301 ataatactgg aattaaaaaa gcttgagagt tatctggtat catatgtgct actgccgtat
      361 gagttttagc tgcattatca tcaatagttt ttcttataat tccagatggg tttaaatcat
      421 aaaagcgaaa acttgctcct gtaagtccat ctataccttt ttttcttaaa ttagtttcta
      481 agcgaaatcc aagtttatga gaaaaaaagt ttgacaaaaa gtaaactagt gcgcctatcg
      541 tcaaatataa gacagcttga atggacaaag tcctagctac taataaatcc ccttcaacaa
      601 ttgaactcct taaaagttta tacagtaacg aatatccgta caccattaaa aatgcagata
      661 gtgctgataa aaaaactgct atatatccat tatgtttt
//
LOCUS       DATUYR010000154          683 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.154, whole genome shotgun sequence.
ACCESSION   DATUYR010000154 DATUYR010000000
VERSION     DATUYR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.154"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            63..683
                     /locus_tag="VCI33_001740"
     CDS             63..683
                     /locus_tag="VCI33_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEP2832621.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
BASE COUNT          244 a          128 c          110 g          201 t
ORIGIN      
        1 atagctatat cttgtttata atttatgata taatacagca aaaatagttt aggaaataac
       61 atatgacaaa aaaacactta ctaacacttc ttctcatctc tttttttact agctttttgg
      121 tagcttgttc aacaacgaaa gataaagagc ctcaaccgtc tgattcagaa atcattactc
      181 cccgactaca ccaagccgct catcaagata aacgcgctaa ctttgaaaaa attaaacttg
      241 cgactgttga ttcctcattt acaggaggga caagccttga agaacttatt tcactctttg
      301 gagagcctag ccaacatgat ccaaaaacag caggcgaagt aacaatcgac gcttatactt
      361 ggcagtttga tcaagttact ctcactgtta atctttatca aaatagtagt attgttaaaa
      421 ccatctctaa ttttaccttt gcaagagagt taggcttatc gcaaaaggaa taccaacaat
      481 tacaaaaagg aatgtcttat gaagacgtta aaaagatctt aacagaacct gataattata
      541 gccaagcgtc atctagtgat catcaaactt tgcaagcgat ttgggttagt ggcttaaaga
      601 cagatacaag cggagctaat atttctctcg tttttgaaaa taatcagtta acagaaatgt
      661 ctcaggtagg acttgaagaa taa
//
LOCUS       DATUYR010000155          666 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.155, whole genome shotgun sequence.
ACCESSION   DATUYR010000155 DATUYR010000000
VERSION     DATUYR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.155"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(212..625)
                     /locus_tag="VCI33_001741"
     CDS             complement(212..625)
                     /locus_tag="VCI33_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP2832622.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRSDKHDYK
                     LDLSCGRIELEIFGKVTSDPNYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
BASE COUNT          239 a          126 c           92 g          209 t
ORIGIN      
        1 acactgtaag agtttatcgt caaatatttt gcttttccta tagttattct attaaaaaac
       61 ttcgacaaga ttactccaaa aattgctctt cactttgaaa gttaaccaaa tcatttaacg
      121 atcaaaagag attaatcaag caaaagaaga tacaacaaat aactattttt aacgagttta
      181 atagcacgat ccagtcgtta acctaataag tctattcatg taattctaaa ggcaaaccat
      241 ccggatcaaa aaagaaagtc attttctcac ctgtgtaatc atcatgccta ataggctcca
      301 ctggaattcc taagctcttt agatcatcta cataactctc aatgttggta acacgaaaag
      361 caaggtgcct caaaccacag gcctcacttt taaattctgg ttgacttaca cgtttaggag
      421 gagcttgata gttggggtca cttgtcacct taccaaaaat ctccaattca atacgaccac
      481 aagataaatc taatttgtaa tcatgcttgt cagaacgata attttctctg ataatttcaa
      541 atccgagttt attcacataa aaatcttttg acagatgata atcagataca ataatagcaa
      601 cgtgatgaat ggcatttagt ttcatcatat ttctcctttc aaaaaaagta ggatgagata
      661 aatttt
//
LOCUS       DATUYR010000156          645 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.156, whole genome shotgun sequence.
ACCESSION   DATUYR010000156 DATUYR010000000
VERSION     DATUYR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.156"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..645)
                     /locus_tag="VCI33_001742"
     CDS             complement(<1..645)
                     /locus_tag="VCI33_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2832623.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRS"
BASE COUNT          214 a          139 c           69 g          223 t
ORIGIN      
        1 gcttcttaaa gtttgaagcc tgttaacaaa acgggtacct tttaattttt tccttatttt
       61 agaaatgtaa acttcaacaa ccgaaatcgt agtatcacta tcgaatcccc ataaacgatc
      121 aaaaatttgt gtctttggta aaataacgtt ttgattttgc aaaagataca ccaacaaatc
      181 aaattctttg ccaagtaatt caactacttt cccttcaacc ttcacctctt ttcgagctaa
      241 gtcgaccact aaattgccaa aactaatatt tttatctgca aatttccctg ttctttttaa
      301 cagtgcttga attcgcattt ttaactcttc taaatagaac ggctttgtca gataatcatc
      361 agcacctaat tcaaatccgt gacctttatc atctaaaccc tctttagctg tcatgatcaa
      421 tacaggaatt ttaatatctt tttctcgcaa ttcttttaag acttgaaagc catttttctc
      481 cggcaacata aggtctaaca aaatcaaatc ataaatacca ctttctgctt cgtataaacc
      541 ttcatcgcca tcaaaaactt gcatgacgtc tgcaaaatca tctaaaaaat caaaaatgga
      601 atttgataag ctcaagtcat cttctactaa taatattttt atcat
//
LOCUS       DATUYR010000157          642 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.157, whole genome shotgun sequence.
ACCESSION   DATUYR010000157 DATUYR010000000
VERSION     DATUYR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.157"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            127..375
                     /locus_tag="VCI33_001743"
     CDS             127..375
                     /locus_tag="VCI33_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP2832624.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            412..>642
                     /locus_tag="VCI33_001744"
     CDS             412..>642
                     /locus_tag="VCI33_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEP2832625.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVI"
BASE COUNT          166 a           97 c          135 g          244 t
ORIGIN      
        1 ctttattatt tagtttatat agtcactttt tgaaagtcga ctattaaaag actattgctt
       61 gaagaataac agctgttatt cttccttgga caaaaggtac aggagtatct aaaaaggaga
      121 attatcatgg gacttatttg gactttaatc gtcggtgcgc ttattggtgt gattgctgga
      181 gcacttacta aaaaaggcgg ttcaatgggc tggattgcaa acatcgctgc tggtctagtt
      241 ggttcttccg ttggtcaagc tttacttggt tcatggggcc cttctttagc aggaatgtct
      301 ttgatcccgt cagttattgg tgccgttatc gttgttatga tcacttcttt tgtactaaac
      361 aaaactaaca actagtttcc tctttattta gtaagtactt acaaatagag aatgttaagt
      421 agaaaagagg tctgtatgtc aaaattgtta aagattagtt attgtcttgt cggacttgtt
      481 ctattgtctg tttttggatg ggtagttgga ataaccggag gctatatata tttaccttat
      541 agttatcgtt ggctaagttg gggtatggat agttttccca atctcttaga ttcagcttta
      601 tcctattatt acttctggac agcccttgta ttatttgtta ta
//
LOCUS       DATUYR010000158          637 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.158, whole genome shotgun sequence.
ACCESSION   DATUYR010000158 DATUYR010000000
VERSION     DATUYR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.158"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>637)
                     /locus_tag="VCI33_001745"
     CDS             complement(<1..>637)
                     /locus_tag="VCI33_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEP2832626.1"
                     /translation="NRLGLEIEKHFGINYNANKMWEAIRFVAHQYEVSPPKQYLQNLK
                     WNGDKNAIRKLLPKYLGADDTELNNWIMEHMILGMIKRIFNPGSKFDEMIVLVGGQGI
                     GKSTFARYLSITDDWFCTIENIQGKDAVMNLMGKTVVEIEEFVALRNAKSANEAKSFL
                     SKLSDRIRIPYEKYATDVPRTCIFIGTLNERTFLNDHTGERRYLPVECNSNQ"
BASE COUNT          186 a          130 c           84 g          237 t
ORIGIN      
        1 ttgattcgag ttacattcaa caggcaaata tcttctttcc ccagtatgat cattaagaaa
       61 agttctttca tttaatgtgc ctatgaatat acatgtccta ggcacatctg ttgcatattt
      121 ttcgtagggt attcttatcc tatcgcttag ttttgataaa aaagatttcg cttcattagc
      181 cgattttgca tttcttaaag caacaaattc ttcgatttca acgacagttt tacccataag
      241 gttcatgacg gcatccttac cttgaatgtt ttctattgta caaaaccaat catctgttat
      301 ggacaagtat cttgcaaaag tcgatttacc tataccttgc ccaccaacta aaacaatcat
      361 ttcatcaaac ttagaaccgg gattaaaaat tctttttatc atgcccaata tcatatgttc
      421 cattatccaa ttgttaagct ctgtatcatc cgctcccaaa tactttggta aaagttttct
      481 aatggcattt ttatctccat tccattttaa attttgaaga tattgctttg gcggactcac
      541 ttcatattgg tgagctacaa aacgaatagc ttcccacatt ttgttggcgt tataattaat
      601 tccaaaatgt ttttctatct ctaagcctag tctatta
//
LOCUS       DATUYR010000159          629 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.159, whole genome shotgun sequence.
ACCESSION   DATUYR010000159 DATUYR010000000
VERSION     DATUYR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.159"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          218 a          126 c           77 g          208 t
ORIGIN      
        1 aaaaataaaa aaatatagct tagagaaagg acgatacccc ataataccta aactatattt
       61 ctatttttat tttttaacaa aaaagtcgac ttatgaaaga agtcgacata gaaaaaaata
      121 ttctcactat atttcaattt aaattgcatg ctcgtatctt cgcacactcc tttcactatg
      181 tttctcaaca tgagctactc agtcgaataa gtccacctta tcctttagct agacaaagtc
      241 taacacaccg cccacaatac catacctaat ccgcctcacc acgttttcag taatcattgt
      301 caacacccct tgcagagtag cagttttagt tttttgctca acacaggcta actaacataa
      361 accttagtgt gcttttgagg tctgaacttt acttgtttag ttgaacacac agtaattact
      421 ataatcatgg ccacaactat tttgcatcaa tttttatagt aaaactaagc taggtaggaa
      481 taactatcta actttttttg cttaaacaag caaaattcaa acttcaaaag ttatcagaca
      541 acttggtata gtaaactaca cttgctttac ttctgaagaa tttcacgcta taataaaagt
      601 gtctatgttt tattatgtgt gttattcaa
//
LOCUS       DATUYR010000160          624 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.160, whole genome shotgun sequence.
ACCESSION   DATUYR010000160 DATUYR010000000
VERSION     DATUYR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.160"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>624
                     /locus_tag="VCI33_001746"
     CDS             <1..>624
                     /locus_tag="VCI33_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEP2832627.1"
                     /translation="TLLIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSR
                     TVYISEGDINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYPYIKGT
                     NAYYSFSYIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVI
                     AVVTLLPMNIYLAASFNQDGFAIGLVLVTIGLFINLLSSKDKSNYNT"
BASE COUNT          211 a           98 c           92 g          223 t
ORIGIN      
        1 gcactttact gatagtgttt ttaccaaaaa agttagaggt tgctacgatc atttttattt
       61 tagtatttgg aacgatatct gctatcatat cacctataaa tgatattcct gatgaatatg
      121 ttcattattc aaggacagtt tatatctcag aaggtgatat taatcttacc aacaataaca
      181 aaaagttaag aatatccaaa gatgttgata agcttattaa acagtcagga aaaacattta
      241 taacatcaaa cttgaaagca actaaacaca gcacaagaga gtatagctat ccttacatca
      301 aaggaactaa tgcatattat agtttttctt atataccaca agctttgggt attttagtag
      361 gaaatgcgct ggatttacct atacttttaa catattattt cggaagattg tgcaacctta
      421 ttagctacgc tatgcttgct tttatagcta tcaagttgtc tggttctttt aaacaagtga
      481 tagctgttgt tactttacta cctatgaaca tttatttagc agcatctttt aatcaagacg
      541 gctttgctat cggtttagtt ttagtaacaa ttggattgtt tattaacttg ctctcatcaa
      601 aagataaatc taactataat acca
//
LOCUS       DATUYR010000161          611 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.161, whole genome shotgun sequence.
ACCESSION   DATUYR010000161 DATUYR010000000
VERSION     DATUYR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.161"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            51..>611
                     /locus_tag="VCI33_001747"
     CDS             51..>611
                     /locus_tag="VCI33_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP2832628.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIF"
BASE COUNT          224 a           84 c          119 g          184 t
ORIGIN      
        1 aaaattgtta aaaaacaagc gtcttgtaaa ccatttcaaa gtatgataga atgaaaaagt
       61 accttaaaaa gataggaaaa atgatgacaa agatgtatta tgatgaaaat ccagatagtc
      121 tacacgatat tcatgaagta aaagtagagc tgcttaatca tccctttact tttttgacgg
      181 attcaggtgt cttttcaaag aaaatggttg atttcggtag tcaagtatta ttagagacac
      241 taaatttcaa ggagaacgaa cgtgtactgg acttaggttg cggctatggt cctcttggga
      301 ttagtctagc caaggttcaa agggtagacg caaccttagt ggacatcaat aatcgtgctc
      361 ttgatttagc tagaaaaaat gcaactaata accaagtggc agtaactatt tttcaatcaa
      421 atatttatga aaatattagt ggccattttg agcatattat tagtaatcct cctattagag
      481 caggaaagag agtagttcat agtattattg aaaaaagcat agatttttta gtggttaatg
      541 gtgacttaac gattgtgatt caaaaaaaac aaggagctcc tagcgctaaa gcaaaaatgg
      601 caactatttt t
//
LOCUS       DATUYR010000162          579 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.162, whole genome shotgun sequence.
ACCESSION   DATUYR010000162 DATUYR010000000
VERSION     DATUYR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.162"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..561)
                     /locus_tag="VCI33_001748"
     CDS             complement(<1..561)
                     /locus_tag="VCI33_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP2832629.1"
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFK
                     GYDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKP
                     FSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYR
                     EYEILELLSRHPYKVFTKEEIYEQVYS"
BASE COUNT          184 a          118 c           67 g          210 t
ORIGIN      
        1 gctatatact tgttcatata tctcttcttt tgtgaaaact ttatacggat gacgtgacag
       61 taattctaaa atttcgtact ctctataagt caggaataac ggctgtttat caatatataa
      121 catacgttcc tctagataga tttctatagg tggaaaactg cgcttcactc tctcctcagc
      181 ctcattcttt atctttgctc tctcttctct cttcaaatga gagttaactt tagctacgaa
      241 ttgttttaag ctaaatggtt tcacaataaa atcgtctccc cctatattta aagcttggac
      301 aatttcatta tcgccatcta aagcgctaac aaaaataatt ggagaatgaa cttcttcccg
      361 aattttataa caaaactcag ttccacttat attaggcatc atgatatcca ataagatcaa
      421 atcatatcct ttaaaatcat caaaaactat tggtaattct atctcttgac aggttataac
      481 atggtaattt tcaatctcca aagcagtctt cataagcttc agaatttctt tatcatcatc
      541 tatggctaaa atcttataca ttattacccc ttttaaata
//
LOCUS       DATUYR010000163          525 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.163, whole genome shotgun sequence.
ACCESSION   DATUYR010000163 DATUYR010000000
VERSION     DATUYR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.163"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..497)
                     /gene="rgg4"
                     /locus_tag="VCI33_001749"
     CDS             complement(<1..497)
                     /gene="rgg4"
                     /locus_tag="VCI33_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEP2832630.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLE"
BASE COUNT          162 a          108 c           65 g          190 t
ORIGIN      
        1 aattctagaa gtattaaatc attttgccta aagcgtacct tagtctttat ttgattaaaa
       61 tattcttgaa gtaagtcaat gccaaagtta gtattctcac tcaataaagt atctaaagtt
      121 gcctgtaaac aatcaatgat tattttctct tcctctggta atttatcata ataatcctca
      181 aaaatttcat caaactgctc ttctcgtact tgtaattttc catcatccat gtaagttggt
      241 gtccttaaga taaggtattt taattctttg taacgcttag gtagttctaa atcagcacca
      301 tcagttaagt aaccgacact aacgtttaag gcttttgcaa taaaaataac cttacttaaa
      361 cttggtatgg attgacctag ttctatccgt gctaattgac gaacagaaag ttcagactca
      421 tccccacaca agtcctcgcg actaatcctc ttttcaagtc ttaacacttt tacttttcca
      481 ccaaaatgtt ctaacatggt ttctctccaa caactgtttt cttta
//
LOCUS       DATUYR010000164          444 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.164, whole genome shotgun sequence.
ACCESSION   DATUYR010000164 DATUYR010000000
VERSION     DATUYR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.164"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>444)
                     /locus_tag="VCI33_001750"
     CDS             complement(<1..>444)
                     /locus_tag="VCI33_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP2832631.1"
                     /translation="TVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKAGDRI
                     AVFSVPFDWNYLSKGKVTAYTYGGITPYQKTSIPKNIPVNLWINRKQISVPYNEISTN
                     KTTVTAQEIDLKVRKFLISQHQLYSSGSSYKSGKLVFHTNDNSDKY"
BASE COUNT          135 a           65 c           72 g          172 t
ORIGIN      
        1 atatttatct gaattatcat ttgtatgaaa aactaattta ccacttttgt agctagaacc
       61 agaagaatat aattgatgtt gtgatattaa aaattttcta acctttagat caatttcttg
      121 agctgtaact gttgttttgt tagttgatat ttcgttgtaa ggaacagaga tctgctttct
      181 attaatccat aaattaacag ggatattttt aggtattgaa gttttttggt agggtgttat
      241 tccgccgtac gtatatgctg tgacttttcc ttttgataaa tagttccaat caaatgggac
      301 ggagaacaca gctattctat ctccagcttt aaaatcattg gctgcatatt cgtccatttc
      361 ggagttaata aaaaaatctc tagcatctct aacatcaagt ttcttagtca ctaagttatg
      421 actggtttta aaatcaatta ctgt
//
LOCUS       DATUYR010000165          435 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.165, whole genome shotgun sequence.
ACCESSION   DATUYR010000165 DATUYR010000000
VERSION     DATUYR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.165"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            120..218
                     /locus_tag="VCI33_001751"
                     /pseudo
     CDS             120..218
                     /locus_tag="VCI33_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
BASE COUNT          124 a          101 c           87 g          123 t
ORIGIN      
        1 ctcccagtac catttgagta ccagtagcca ttttttacaa ctttcctctc gaaaaccatt
       61 ctggttttct cgcttaattg gtatggatac aataaatagc acaatttatt gtatccatac
      121 cagatcgcct gacagatgat gatgatgatg tcatgctgtt gttgaaactc atttttcaca
      181 ggcaggacac gcaggaacta ttgaaaaaac agctgtaacg ggagaagcct gaaatccctt
      241 agtttactaa gggcgtaatt atacaccacc cagaggttgg tgcattgtgt tctccgaagg
      301 ctcctcgcca gagggctgtg attttccgca gtcatggtct gttccaaatc aaaaagagga
      361 cactacgctt cccctgcact ggctgccgcc agctaccctt tgaaaggaaa aatttataga
      421 aacagtattt actac
//
LOCUS       DATUYR010000166          341 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.166, whole genome shotgun sequence.
ACCESSION   DATUYR010000166 DATUYR010000000
VERSION     DATUYR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.166"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>341
                     /locus_tag="VCI33_001752"
     CDS             <1..>341
                     /locus_tag="VCI33_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEP2832632.1"
                     /translation="GNILVYGRLKYFKEGISLYYIGEYLAECLLIEHSDTLLIHAAAV
                     YNPLSGHSILLLGDKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSNSGILLTYAGTSFF
                     KIRRTAVLSDN"
BASE COUNT          111 a           47 c           67 g          116 t
ORIGIN      
        1 ggaaatattt tagtttatgg aagattaaag tattttaaag aaggaatttc attatactac
       61 attggagagt acttagctga atgtctcttg attgaacata gtgacacctt attaattcat
      121 gcagcagcag tttataatcc tttatctggc cattcaattt tactattagg agataaaggt
      181 gctggaaaaa ctacggttgc aataaggctg tgtatagaac atgggtatca ccttattggt
      241 aatgaccaag taatatttgg ttcgaactct ggaatactac taacttatgc agggacatct
      301 ttctttaaaa taagaaggac agctgttttg tcggacaatt t
//
LOCUS       DATUYR010000167          339 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28774
            SAMN08562570-rid19751903.denovo.167, whole genome shotgun sequence.
ACCESSION   DATUYR010000167 DATUYR010000000
VERSION     DATUYR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08562570
            Sequence Read Archive: SRR6747505
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001919844.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001919844.2
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 13:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,752
            CDSs (total)                      :: 1,716
            Genes (coding)                    :: 1,671
            CDSs (with protein)               :: 1,671
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 26 of 45
            Pseudo Genes (incomplete)         :: 22 of 45
            Pseudo Genes (internal stop)      :: 9 of 45
            Pseudo Genes (multiple problems)  :: 11 of 45
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08562570-rid19751903.denovo.167"
                     /strain="MGAS28774"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Maryland"
                     /collection_date="2012"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          135 a           45 c           50 g          109 t
ORIGIN      
        1 tagaattttt tctaactaaa aataatagca caatctttgg cagcaatctg aattgttttt
       61 ccataaaaga tttaagcact tacgtttgct taaaatatag gttatgtgac gtgcgtgcaa
      121 gatcagatta taacaaaata gttccaaata agcctatcac tgagaaagca tcacagaagg
      181 gttaaacttt ttttgtatta gtgttttatc agtacaacct aacacttatt tttataaaaa
      241 aataaaaaag ctattgacaa tcttatacaa gataacgtat actaataaag ctgttgtaaa
      301 tatggtaaag caatagatgc tatcaggaaa aaatgtgaa
//
